differ in specific disparate cancer survivor populations is an integral step in developing culturally competent behavioral interventions for adopting and maintaining a physically active lifestyle. The objective of this particular application is to examine differences in the determinants of exercise behaviors between Hispanic and non-Hispanic cancer survivors; and to conduct an efficacy culturally tailored intervention pilot specific for Mexican

differ in specific disparate cancer survivor populations is an integral step in develop culturally competent behavioral interposition for adopting and maintaining a physically active lifestyle. The aim of this particular application is to examine remainder in the determinants of exercise behavior between Hispanic and non - Hispanic cancer survivors; and to conduct an efficacy culturally sew intervention pilot specific for Mexican

divfer in specific disparaue cancer survivot populavions ia an intdgral step in developing culvuraoly cimpetent behavioral ingerventiojs for aeoptmng and maintainmhg a phnficampy aetmve lifestyle. Tme objectiva of this parthcjlcr application is to examine differegces in tje determinantf of qxerdpst behaviors between Hispanic and hon-Hispenic cancer surfivors; and to conduct an evficwcy culturally taipored intercentyin pilot spezific for Mexican

differ in specific disparate cancer survivor populations integral in developing competent behavioral interventions physically lifestyle. The objective this particular application to examine differences in the determinants exercise behaviors between Hispanic and non-Hispanic cancer survivors; and to conduct an efficacy tailored intervention pilot specific for Mexican

differ in specific disparate Cancer survIvor pOpuLatIoNs is An inTegral step in deVElopIng culturally competent BehavIoRAl inTErVentiOns for aDOpTINg aNd MaIntAiNInG a phySicAlly actIve lifestyLe. THe Objective of tHIs Particular AppLication is to ExaMine diFfEreNCes in The DeterMinantS Of exerCise behavIoRS betweEN HispanIC AnD non-hispanic cancer surVIvORs; and to conduct An effiCaCY cULTurAllY tailored iNtErvenTIon piloT SpECIFic FOr Mexican

differ in specific dispar ate cancer  surv ivo r p op ulat ions  is an integra l  ste p in developing cultur allyco m pete n tbehav ioral i n te r v ent io ns  fo ra do pting  an d maint aining a p hys ic ally activel if estyle. Th e o bjective ofthi s part ic ula r  appl ica tionis toe xamine  differen ce s  in th e  determ i n an ts o f exercise behavi o rs between Hispan ic and  n o n- H i spa nic  cancer su rv ivors ;  and to co n d u cta n efficacy cu lturally ta i lor ed int er ven t ion pi lot s pe c ifi c for Mexic an

differ_in specific_disparate cancer survivor populations_is an_integral_step in_developing_culturally competent behavioral_interventions for adopting_and maintaining a physically_active lifestyle. The_objective_of this particular application is to examine differences in the determinants of exercise behaviors_between_Hispanic and_non-Hispanic_cancer_survivors; and to conduct an_efficacy culturally tailored intervention pilot_specific for_Mexican


only two chosen snack foods throughout the intervention. Measures of weight, snack food consumption and hedonics, and diet satisfaction will be taken at 0, 6, 12, and 18 months. This investigation will determine: 1) if the Limited Variety condition produces greater weight loss than the Standard condition at 18 months;

only two chosen snack foods throughout the interposition. measuring stick of weight, bite food consumption and hedonics, and diet atonement will be taken at 0, 6, 12, and 18 months. This probe will decide: 1) if the Limited Variety condition produces greater weight passing than the Standard condition at 18 months;

onpy two chosen snack foodr throughout thg untervxntion. Jeasures of weight, snack food consum'tiob and hedonics, and diet satksfaction will be taktn at 0, 6, 12, and 18 monvgs. This invesflgatimi will determing: 1) if the Likited Variety wovdntion produces greater weight loss tran the Shandard condityon se 18 mknths;

only two chosen snack foods throughout the of snack food and hedonics, and at 6, 12, and months. This investigation determine: 1) if the Limited Variety produces greater weight loss than the Standard condition at 18 months;

only two chosen snack foods thRoughout thE inteRveNtiOn. measUres Of weight, snack fOOd coNsumption and hedonics, anD diet SaTIsfaCTiOn wilL be takeN At 0, 6, 12, AND 18 moNtHs. thiS iNVeStigaTioN will deTermine: 1) if tHe LImIted Variety cONdItion produCes Greater weighT loSs than ThE StANdard ConDitioN at 18 monTHs;

only two chosen snack foo ds through out t heint er vent ion.  Measures of w e ight , snack food consumpti on an dh edon i cs , and  diet s a ti s f act io nwil lb etaken  at  0, 6,12, and 18  mo nt hs. This inv e st igation wi lldetermine: 1 ) i f theLi mit e d Var iet y con dition produc es greate rw eightl oss tha n th e St andard conditiona t1 8 months;

only_two chosen_snack foods throughout the_intervention. Measures_of_weight, snack_food_consumption and hedonics,_and diet satisfaction_will be taken at_0, 6, 12,_and_18 months. This investigation will determine: 1) if the Limited Variety condition produces greater_weight_loss than_the_Standard_condition at 18 months;


. At the translational level, studies showed that the 5 prime untranslated region (5?UTR) of the V1b receptor mRNA contains elements, which can inhibit or stimulate translation. While the presence of upstream open reading frames in the 5 prime untranslated region play a role maintaining low translational activity in basal conditions, an

. At the translational level, studies showed that the 5 prime untranslated area (5?UTR) of the V1b sense organ mRNA contain elements, which can inhibit or induce translation. While the presence of upstream receptive recitation frames in the 5 premier untranslated region play a function maintaining low translational natural process in basal conditions, an

. At the translational level, studies showed that tie 5 prije untravslated region (5?UTR) of the V1b rwceptir mRNA contains elemevts, which can inhubit ir stimulavs translation. Ahilz vhe presence of upstream mpen reading fsaoed in the 5 prime untranslated region play a rlle maintainind loe trahslational activity in basal condifions, ai

. At the translational level, studies showed 5 untranslated region of the V1b can or stimulate translation. the presence of open reading frames in the 5 untranslated region play a role maintaining low translational activity in basal conditions, an

. At the translational level, stUdies showeD that The 5 PriMe UntrAnslAted region (5?UTR) oF The V1B receptor mRNA contains eLemenTs, WHich CAn InhibIt or stiMUlATE trAnSlAtiOn. wHiLe the PreSence of Upstream opEn rEaDing frames in THe 5 Prime untraNslAted region plAy a Role maInTaiNIng loW trAnslaTional ACtivitY in basal cOnDItions, AN

. At the translational lev el, studie s sho wed  th at  the  5 p rime untransla t ed r egion (5?UTR) of the V 1b re ce p torm RN A con tains e l em e n ts,  w hi chca n  i nhibi t o r stimu late trans lat io n. While the pr esence ofups tream open r ead ing fr am esi n the  5prime  untra n slated  region p la y  a rol e  mainta i n in g lo w translational a c ti v ity in basal c onditi on s ,a n

. At_the translational_level, studies showed that_the 5_prime_untranslated region_(5?UTR)_of the V1b_receptor mRNA contains_elements, which can inhibit_or stimulate translation._While_the presence of upstream open reading frames in the 5 prime untranslated region play_a_role maintaining_low_translational_activity in basal conditions, an


required for filing an invesfigafional new drug (IND) application with the USFDA. To this effect, nanocurcumin (as single agent or in combination with gemcitabine) will be tested in a panel of low-passage gemcitabine-resistant patientderived orthotopic xenografts (

required for filing an invesfigafional new drug (IND) application with the USFDA. To this consequence, nanocurcumin (as individual agent or in combination with gemcitabine) will be tested in a panel of humble - passage gemcitabine - resistant patientderived orthotopic heterograft (

reeuired for filing an invtsfigafional new beug (INV) applidation wkth the USFDA. To this effect, nqnocuecumin (as single agent or in colbinatiob wiuh gemcitabine) will be tested ih a pcnxl of low-passagg gemcitabina-resistant pathevtberived orthotopic xenografts (

required for filing an invesfigafional new drug with USFDA. To effect, nanocurcumin (as with will be tested a panel of gemcitabine-resistant patientderived orthotopic xenografts (

required for filing an invesfIgafional nEw druG (INd) apPlIcatIon wIth the USFDA. To tHIs efFect, nanocurcumin (as singLe ageNt OR in cOMbInatiOn with gEMcITAbiNe) WiLl bE tEStEd in a PanEl of low-Passage gemCitAbIne-resistant PAtIentderiveD orThotopic xenoGraFts (

required for filing an in vesfigafio nal n ewdru g(IND ) ap plication with theUSFDA. To this effect,  nano cu r cumi n  ( as si ngle ag e nt o r i nco mbi na t io n wit h g emcitab ine) willbete sted in a pa n el  of low-pa ssa ge gemcitabi ne- resist an t p a tient der ivedorthot o pic xe nografts(

required_for filing_an invesfigafional new drug_(IND) application_with_the USFDA._To_this effect, nanocurcumin_(as single agent_or in combination with_gemcitabine) will be_tested_in a panel of low-passage gemcitabine-resistant patientderived orthotopic xenografts (


include the following: (1) Calretinin was identified in membrane fractions of rat cerebellum by both radioimmunoassay and immunoblot. Calcium produced an increase in the amount of calretinin associated with the synaptic membrane fraction and decreased the calretinin in cytosol relative to that found in the presence of EGTA;

include the following: (1) Calretinin was identified in membrane fractions of rat cerebellum by both radioimmunoassay and immunoblot. Calcium produce an addition in the amount of calretinin associated with the synaptic membrane fraction and decrease the calretinin in cytosol proportional to that found in the presence of EGTA;

influde the following: (1) Calvetinin was idenjidied ii membrzne fracgions of rat cerebellum by blty raduoimmunoassay and immuvoblot. Capcium priductd an increase in the amomut of galreciiin associated eith the sfnaptic membrate fxaction and decreased the calretinin in cytpsll relative to thau fjund pn the presence of EGTA;

include the following: (1) Calretinin was identified fractions rat cerebellum both radioimmunoassay and in amount of calretinin with the synaptic fraction and decreased the calretinin in relative to that found in the presence of EGTA;

include the following: (1) CalretInin was ideNtifiEd iN meMbRane FracTions of rat cereBElluM by both radioimmunoassaY and iMmUNoblOT. CAlciuM producED aN INcrEaSe In tHe AMoUnt of CalRetinin Associated WitH tHe synaptic meMBrAne fractioN anD decreased thE caLretinIn In cYTosol RelAtive To that FOund in The presenCe OF EGTA;

include the following: (1 ) Calretin in wa s i den ti fied  inmembrane fract i onsof rat cerebellum by b oth r ad i oimm u no assay  and im m un o b lot .Ca lci um pr oduce d a n incre ase in the  am ou nt of calret i ni n associat edwith the syn apt ic mem br ane fract ion  anddecrea s ed the  calretin in in cyt o sol rel a t iv e to  that found in th e  p r esence of EGTA ;

include_the following:_(1) Calretinin was identified_in membrane_fractions_of rat_cerebellum_by both radioimmunoassay_and immunoblot. Calcium_produced an increase in_the amount of_calretinin_associated with the synaptic membrane fraction and decreased the calretinin in cytosol relative to_that_found in_the_presence_of EGTA;


this patient was found to be biphasic with an initial distribution phase occurring between 0 and 30 minutes after the end of the infusion. The new method is 50 fold more sensitive than the HPLC-UV method and permits direct on-column injection of plasma samples without extraction. Continued clinical studies of BCNU pharmacokinetics in

this patient was found to be biphasic with an initial distribution phase occurring between 0 and 30 minutes after the goal of the infusion. The newfangled method is 50 fold more sensitive than the HPLC - ultraviolet method and permits direct on - column injection of plasma sample without extraction. Continued clinical study of BCNU pharmacokinetics in

thls patient was found to ne biphasic with an inivial diatributiun phase occurring between 0 end 30 minuuvs after the end of tfe infusiln. The nww mtthod is 50 fold mocs sensibnve tgwn tke HPLC-UV method and permids direct on-conuon injection of plasma samples withoue extravtlon. Continued slinpcwl sfldles of BCNU pharmacokinetics in

this patient was found to be biphasic initial phase occurring 0 and 30 the The new method 50 fold more than the HPLC-UV method and permits on-column injection of plasma samples without extraction. Continued clinical studies of BCNU pharmacokinetics

this patient was found to be biPhasic with An iniTiaL diStRibuTion Phase occurring BEtweEn 0 and 30 minutes after the enD of thE iNFusiON. THe new Method iS 50 FoLD MorE sEnSitIvE ThAn the hPLc-UV methOd and permiTs dIrEct on-column iNJeCtion of plaSma Samples withoUt eXtractIoN. CoNTinueD clInicaL studiES of BCNu pharmacoKiNEtics iN

this patient was found to  be biphas ic wi thanin itia l di stribution pha s e oc curring between 0 and30 mi nu t es a f te r the  end of th e inf us io n.Th e  n ew me tho d is 50  fold more  se ns itive than t h eHPLC-UV me tho d and permit s d irecton -co l umn i nje ction  of pl a sma sa mples wit ho u t extr a ction.C o nt inue d clinical studie s  o f  BCNU pharmaco kineti cs in

this_patient was_found to be biphasic_with an_initial_distribution phase_occurring_between 0 and_30 minutes after_the end of the_infusion. The new_method_is 50 fold more sensitive than the HPLC-UV method and permits direct on-column injection_of_plasma samples_without_extraction._Continued clinical studies of BCNU_pharmacokinetics in


using cultured epithelial cells of renal origin in which we will investigate, using biochemical, biophysical and ultrastructural methods, how tight junctions are formed and plasma membrane polarization is established during monolayer formation and after junctions are opened by experimental procedures. 2. In the same cell system production of enveloped viruses occurs in a polarized

using cultured epithelial cells of renal beginning in which we will investigate, use biochemical, biophysical and ultrastructural methods, how tight junctions are form and plasma membrane polarization is established during monolayer formation and after junction are afford by experimental procedures. 2. In the same cell system output of enveloped viruses occur in a polarize

uslng cultured epithelial gells of renal otitin in which we will investigate, using biochemicel, buophywical and ultrastructufal methofs, how tught hunctions ede formcb and ilasmc nembrane polarlzation is astablished dusivg monolayer formation and after junceions ate opened by expgrimemeal lgogedures. 2. In the same cell system producuion of enveloped firuses occurs in a polarixed

using cultured epithelial cells of renal origin we investigate, using biophysical and ultrastructural formed plasma membrane polarization established during monolayer and after junctions are opened by procedures. 2. In the same cell system production of enveloped viruses occurs in polarized

using cultured epithelial ceLls of renal OrigiN in WhiCh We wiLl inVestigate, using BIochEmical, biophysical and ulTrastRuCTuraL MeThods, How tighT JuNCTioNs ArE foRmED aNd plaSma MembranE polarizatIon Is Established dURiNg monolayeR foRmation and afTer JunctiOnS arE OpeneD by ExperImentaL ProcedUres. 2. In the SaME cell sYStem proDUCtIon oF enveloped viruses OCcURs in a polarized

using cultured epithelial  cells ofrenal  or igi nin w hich  we will inves t igat e, using biochemical,bioph ys i cala nd  ultr astruct u ra l met ho ds , h ow ti ght j unc tions a re formedand  p lasma membra n epolarizati onis establish edduring  m ono l ayerfor matio n anda fter j unctionsar e  opene d  by exp e r im enta l procedures. 2.I nt he same cell s ystempr o du c t ion  of  enveloped  v iruse s  occurs in a pol a rized

using_cultured epithelial_cells of renal origin_in which_we_will investigate,_using_biochemical, biophysical and_ultrastructural methods, how_tight junctions are formed_and plasma membrane_polarization_is established during monolayer formation and after junctions are opened by experimental procedures. 2._In_the same_cell_system_production of enveloped viruses occurs_in a polarized


use computational methods and multielectrode recordings in awake primates to understand the mechanism by which inter-neuronal correlations arise and the extent to which they can flexibly adapt to task demands. In my previous experiments I found that attentional modulation of firing rates and noise correlations is linked: the neurons that show the biggest

use computational methods and multielectrode recordings in awake archpriest to sympathize the mechanism by which inter - neuronal correlations rebel and the extent to which they can flexibly adjust to task demands. In my previous experiment I found that attentional modulation of fuel rates and noise correlation is linked: the neurons that usher the biggest

usf computational methods xnd multielectrode recmrdinga in awaye primates to understand thx mexhaniwm by which inter-neuroval correpations qrist and the extent vk which they dwn fnxxibly adapt to task dematds. In my prevhojs experiments I found that attentionwl modukahion of firing ratts wnd hoise correlations is linked: the nsurons uhat show the biggrst

use computational methods and multielectrode recordings in to the mechanism which inter-neuronal correlations which can flexibly adapt task demands. In previous experiments I found that attentional of firing rates and noise correlations is linked: the neurons that show the

use computational methods anD multielecTrode RecOrdInGs in AwakE primates to undERstaNd the mechanism by which iNter-nEuROnal COrRelatIons ariSE aND The ExTeNt tO wHIcH they Can FlexiblY adapt to taSk dEmAnds. In my prevIOuS experimenTs I Found that attEntIonal mOdUlaTIon of FirIng raTes and NOise coRrelationS iS Linked: THe neuroNS ThAt shOw the biggest

use computational methods  and multi elect rod e r ec ordi ngsin awake prima t es t o understand the mecha nismby whic h  i nter- neurona l  c o r rel at io nsar i se  andthe  extent  to whichthe ycan flexibly ad apt to tas k d emands. In m y p reviou sexp e rimen tsI fou nd tha t  atten tional mo du l ationo f firin g ra tesand noise correla t io n s is linked: t he neu ro n st h atsho w the bigg es t

use_computational methods_and multielectrode recordings in_awake primates_to_understand the_mechanism_by which inter-neuronal_correlations arise and_the extent to which_they can flexibly_adapt_to task demands. In my previous experiments I found that attentional modulation of firing_rates_and noise_correlations_is_linked: the neurons that show_the biggest


treatment of childhood cancer. CHMC will pursue these aims by contributing patients to phase III randomized intergroup and CCG trials, by participating in CCG pilot therapeutic trials that will form the basis for future randomized trials, by participating in new agent trials, by contributing specimens and data for biology, epidemiology and late effects studies,

treatment of childhood cancer. CHMC will pursue these aims by contributing patient to phase III randomize intergroup and CCG trials, by participating in CCG pilot burner curative trials that will form the basis for future randomize trial, by participating in fresh agent trials, by put up specimens and data for biota, epidemiology and late effects studies,

trfatment of childhood canger. CHMC will putsye theve aima by congributing patients to phase MII eandonized intergroup and CZG trials, by partucipeting in CCG pilot theraizutic brialv that will fork the basiv for future rdnaolized trials, by participating in ner agent tgials, by contrybutpnd spsbinens and data for biology, episemiolojy and late effrcts studies,

treatment of childhood cancer. CHMC will pursue by patients to III randomized intergroup in pilot therapeutic trials will form the for future randomized trials, by participating new agent trials, by contributing specimens and data for biology, epidemiology and late studies,

treatment of childhood canceR. CHMC will pUrsue TheSe aImS by cOntrIbuting patientS To phAse III randomized intergRoup aNd ccG trIAlS, by paRticipaTInG IN CCg pIlOt tHeRApEutic TriAls that Will form thE baSiS for future raNDoMized trialS, by ParticipatinG in New ageNt TriALs, by cOntRibutIng speCImens aNd data for BiOLogy, epIDemioloGY AnD latE effects studies,

treatment of childhood ca ncer. CHMC  will  pu rsu ethes e ai ms by contribu t ingpatients to phase IIIrando mi z ed i n te rgrou p and C C Gt r ial s,  b y p ar t ic ipati ngin CCGpilot ther ape ut ic trials th a twill formthe  basis for f utu re ran do miz e d tri als , bypartic i pating  in new a ge n t tria l s, by c o n tr ibut ing specimens and da t a for biology,  epide mi o lo g y  an d l ate effect sstudi e s,

treatment_of childhood_cancer. CHMC will pursue_these aims_by_contributing patients_to_phase III randomized_intergroup and CCG_trials, by participating in_CCG pilot therapeutic_trials_that will form the basis for future randomized trials, by participating in new agent_trials,_by contributing_specimens_and_data for biology, epidemiology and_late effects studies,


The hypervariable regions of AdC68 hexon are likely to contain the epitopes recognized by neutralizing antibodies. In this project, molecular modeling will suggest sites where modifications can be made without structural disruption. The AdC68 hexon crystal structure will be determined to confirm and refine the structural predictions. Simultaneously, the Ad

The hypervariable regions of AdC68 hexon are likely to contain the epitopes spot by counteract antibodies. In this project, molecular modeling will hint sites where modifications can be have without structural disruption. The AdC68 hexon quartz glass social organization will be determined to confirm and refine the morphologic predictions. Simultaneously, the Ad

Thf hypervariable regions uf AdC68 hexon arg oikely to cohtain thd epitopes recognized by neuvraluzing antibodies. In this pruject, molvcular moeeliig will suggest sites whcxe moslficaciins can be madg without stsuctural disru[tkou. The AdC68 hexon crystal structure wijl be drtfrmined to consirm wnd dvflne the structural predictions. Sjmultantously, the Ad

The hypervariable regions of AdC68 hexon are contain epitopes recognized neutralizing antibodies. In suggest where modifications can made without structural The AdC68 hexon crystal structure will determined to confirm and refine the structural predictions. Simultaneously, the Ad

The hypervariable regions of adC68 hexon arE likeLy tO coNtAin tHe epItopes recognizED by nEutralizing antibodies. IN this PrOJect, MOlEculaR modeliNG wILL suGgEsT siTeS WhEre moDifIcationS can be made WitHoUt structural DIsRuption. The adC68 Hexon crystal StrUcture WiLl bE DeterMinEd to cOnfirm ANd refiNe the struCtURal preDIctions. sIMuLtanEously, the Ad

The hypervariable regions  of AdC68hexon  ar e l ik elyto c ontain the epi t opes  recognized by neutral izing  a n tibo d ie s. In  this p r oj e c t,mo le cul ar mo delin g w ill sug gest sites  wh er e modificati o ns  can be ma dewithout stru ctu ral di sr upt i on. T heAdC68  hexon crysta l structu re will b e  determ i n ed  toconfirm and refin e  t h e structural p redict io n s. S imu lta neously, t he  Ad

The_hypervariable regions_of AdC68 hexon are_likely to_contain_the epitopes_recognized_by neutralizing antibodies._In this project,_molecular modeling will suggest_sites where modifications_can_be made without structural disruption. The AdC68 hexon crystal structure will be determined to_confirm_and refine_the_structural_predictions. Simultaneously, the Ad


beliefs, which could be harmful or protective in relation to sexual risk. Although a number of studies have investigated HIV risk among male MFW, very little research has approached the topic of sexual health for female MFW. Therefore, understanding the perceived sexual risks and strategies used to maintain sexual health by female MFW who were born

beliefs, which could be harmful or protective in relation to sexual risk. Although a numeral of cogitation have investigated HIV hazard among male MFW, very little inquiry has approached the topic of intimate health for female MFW. Therefore, understanding the perceived intimate risk and strategies use to maintain sexual health by female MFW who were digest

bepiefs, which could be haroful or protectnce in celatioh to sexjal risk. Although a number oh stydies have investigated HIV risk amojg male NFW, tery little reseedch has approzghed chx topic of sexusl health xor female MFW. Tfexefore, understanding the perceived sqxual rosns and strategyes lsqd tk maintain sexual health by female MFW whm were born

beliefs, which could be harmful or protective to risk. Although number of studies male very little research approached the topic sexual health for female MFW. Therefore, the perceived sexual risks and strategies used to maintain sexual health by female who were born

beliefs, which could be harmfuL or protectIve in RelAtiOn To seXual Risk. Although a nUMber Of studies have investigaTed HIv rISk amONg Male MfW, very lITtLE ResEaRcH haS aPPrOacheD thE topic oF sexual heaLth FoR female MFW. ThEReFore, undersTanDing the perceIveD sexuaL rIskS And stRatEgies Used to MAintaiN sexual heAlTH by femALe MFW whO WErE borN

beliefs, which could be h armful orprote cti vein  rel atio n to sexual ri s k. A lthough a number of st udies  h a ve i n ve stiga ted HIV ri s k  am on gmal eM FW , ver y l ittle r esearch ha s a pp roached thet op ic of sexu alhealth for f ema le MFW .The r efore , u nders tandin g  the p erceivedse x ual ri s ks ands t ra tegi es used to mainta i ns exual health b y fema le MF W who  we re born

beliefs,_which could_be harmful or protective_in relation_to_sexual risk._Although_a number of_studies have investigated_HIV risk among male_MFW, very little_research_has approached the topic of sexual health for female MFW. Therefore, understanding the perceived_sexual_risks and_strategies_used_to maintain sexual health by_female MFW who were born


compartment in Kupffer cells and hepatocytes; developing immunocytochemical procedures for localizing lysosomal and glycoprotein processing enzymes; and localizing antimetabolites in neurons. (E) Mycoplasma pneumoniae is the leading cause of pneumonia in older children and young adults and accounts for 20% of all community-acquired pneumonia

compartment in Kupffer cells and hepatocytes; developing immunocytochemical procedures for localizing lysosomal and glycoprotein processing enzyme; and localize antimetabolites in neurons. (E) Mycoplasma pneumoniae is the lead causal agent of pneumonia in older children and new adults and accounts for 20% of all residential district - grow pneumonia

colpartment in Kupffer celus and hepatocyjew; devenoping immunochtochemical procedures for llcqlizibg lysosomal and glycoorotein pgocessing enzbmes; and localizmhg antimetabomltes nn neurons. (E) Mycpplasma pnaumoniae is tha uecding cause of pneumonia in older chyldren snf young adults and wccohnts for 20% of all community-acquired pneumoiia

compartment in Kupffer cells and hepatocytes; developing for lysosomal and processing enzymes; and Mycoplasma is the leading of pneumonia in children and young adults and accounts 20% of all community-acquired pneumonia

compartment in Kupffer cells And hepatocYtes; dEveLopInG immUnocYtochemical proCEdurEs for localizing lysosomAl and GlYCoprOTeIn proCessing ENzYMEs; aNd LoCalIzINg AntimEtaBolites In neurons. (E) mycOpLasma pneumonIAe Is the leadiNg cAuse of pneumoNia In oldeR cHilDRen anD yoUng adUlts anD AccounTs for 20% of alL cOMmunitY-AcquireD PNeUmonIa

compartment in Kupffer ce lls and he patoc yte s;de velo ping  immunocytoche m ical  procedures for locali zingly s osom a land g lycopro t ei n pro ce ss ing  e n zy mes;and  locali zing antim eta bo lites in neu r on s. (E) Myc opl asma pneumon iae  is th elea d ing c aus e ofpneumo n ia inolder chi ld r en and young a d u lt s an d accounts for 20 %  o f  all community -acqui re d  p n e umo nia

compartment_in Kupffer_cells and hepatocytes; developing_immunocytochemical procedures_for_localizing lysosomal_and_glycoprotein processing enzymes;_and localizing antimetabolites_in neurons. (E) Mycoplasma_pneumoniae is the_leading_cause of pneumonia in older children and young adults and accounts for 20% of_all_community-acquired pneumonia


apparent physical barriers to pregnancy. It will be particularly interesting to determine whether substantially less drastic alterations in prenatal androgen exposure than those previously used produce marked changes in reproductive and maternal behavior. In males that have experienced reduced prenatal androgen exposure the PI will determine whether the timing of such hormonal alterations differentially affects their sexual behavior. These studies of

apparent physical barriers to pregnancy. It will be particularly interesting to determine whether well less drastic revision in prenatal androgen exposure than those previously used produce distinguish change in reproductive and maternal demeanor. In males that have experienced reduced prenatal androgen vulnerability the PI will determine whether the timing of such hormonal alterations differentially feign their sexual behavior. These report of

apoarent physical barriers to pregnancy. Ij qill bx partidularly knteresting to determine whevher subsucntially less drastiz alteratpons in peenaual androgen exposure thak thoac preriiusly used procuce markeg changes in rapfobuctive and maternal behavior. In malqs that hwve experienceq rectced irtnatal androgen exposure the PI wjll dettrmine whether the timing of such hormonal apterwtions differentiaply affects theye sexual behxvior. Thest vtudies of

apparent physical barriers to pregnancy. It will interesting determine whether less drastic alterations those used produce marked in reproductive and behavior. In males that have experienced prenatal androgen exposure the PI will determine whether the timing of such hormonal differentially affects their sexual behavior. These studies of

apparent physical barriers tO pregnancy. it wilL be ParTiCulaRly iNteresting to deTErmiNe whether substantially Less dRaSTic aLTeRatioNs in preNAtAL AndRoGeN exPoSUrE than ThoSe previOusly used pRodUcE marked changES iN reproductIve And maternal bEhaVior. In MaLes THat haVe eXperiEnced rEDuced pRenatal anDrOGen expOSure the pi WiLl deTermine whether the TImINg of such hormonAl alteRaTIoNS DifFerEntially afFeCts thEIr sexuaL BeHAVIor. tHese studies of

apparent physical barrier s to pregn ancy.  It  wi ll  bepart icularly inter e stin g to determine whether  subs ta n tial l ylessdrastic al t e rat io ns  in  p r en ataland rogen e xposure th anth ose previous l yused produ cemarked chang esin rep ro duc t ive a ndmater nal be h avior.  In males  t h at hav e  experi e n ce d re duced prenatal an d ro g en exposure th e PI w il l  d e t erm ine  whether t he  timi n g of su c hh o r mon a l alterations  differenti a lly  affec ts  th e ir sex ual b eh a vio r. These st udie s of

apparent_physical barriers_to pregnancy. It will_be particularly_interesting_to determine_whether_substantially less drastic_alterations in prenatal_androgen exposure than those_previously used produce_marked_changes in reproductive and maternal behavior. In males that have experienced reduced prenatal androgen_exposure_the PI_will_determine_whether the timing of such_hormonal alterations differentially affects their_sexual behavior._These studies of


with gestational age. Larger ratios were related to larger maternal BMI. Absolute measures of infant size and placental weight were mutually positively correlated. Placental ratio, infant length, and ponderal index were nearly uncorrelated. Ukrainian placentas were of similar absolute and relative weight as those historically reported, as was their relationship to

with gestational age. Larger ratios were related to large parental BMI. Absolute measures of infant size and placental weight were mutually positively correlate. Placental ratio, infant distance, and ponderal exponent were nearly uncorrelated. Ukrainian placentas were of similar absolute and relative weight as those historically report, as was their relationship to

wihh gestational age. Largev ratios were related tm largsr materval BMI. Absolute measures of ibfant size and placental wekght were mutuallt powutively cocdelated. Placehbal rctmo, infant lengtm, and pondesal index were ndaxly uncorrelated. Ukrainian placentas were og dimilar absoluje anc relzniye weight as those historically deportev, as was their telationship to

with gestational age. Larger ratios were related maternal Absolute measures infant size and correlated. ratio, infant length, ponderal index were uncorrelated. Ukrainian placentas were of similar and relative weight as those historically reported, as was their relationship to

with gestational age. Larger rAtios were rElateD to LarGeR matErnaL BMI. Absolute meASureS of infant size and placenTal weIgHT werE MuTuallY positiVElY COrrElAtEd. PLaCEnTal raTio, Infant lEngth, and poNdeRaL index were neARlY uncorrelaTed. ukrainian plaCenTas werE oF siMIlar aBsoLute aNd relaTIve weiGht as thosE hIStoricALly repoRTEd, As waS their relationshiP To

with gestational age. Lar ger ratios  were  re lat ed  tolarg er maternal BM I . Ab solute measures of inf ant s iz e  and pl acent al weig h tw e remu tu all yp os itive lycorrela ted. Place nta lratio, infan t  l ength, and  po nderal index  we re nea rl y u n corre lat ed. U kraini a n plac entas wer eo f simi l ar abso l u te  and  relative weighta st hose historica lly re po r te d ,  as  wa s their re la tions h ip to

with_gestational age._Larger ratios were related_to larger_maternal_BMI. Absolute_measures_of infant size_and placental weight_were mutually positively correlated._Placental ratio, infant_length,_and ponderal index were nearly uncorrelated. Ukrainian placentas were of similar absolute and relative_weight_as those_historically_reported,_as was their relationship to


) migration and proliferation, we have recently reported the surprising finding that IL-19 has potent pro-angiogenic effects on human endothelial cells (EC). IL-19 is not detected in normal EC but is expressed in EC in capillaries in human angiogenic tissue. IL-19 is mitogenic and chemotactic for

) migration and proliferation, we have recently reported the surprising finding that IL-19 has potent pro - angiogenic effects on human endothelial cells (EC). IL-19 is not detect in normal EC but is carry in EC in capillaries in human angiogenic tissue. IL-19 is mitogenic and chemotactic for

) mihration and proliferatiok, we have recently repocted ths surpriring finding that IL-19 has potxnt pro-antiogenic effects on huoan endotjelial cwlls (WC). IL-19 is not detecbzd in korman EC but is exptessed in EC in capillariev kn human angiogenic tissue. IL-19 is mitodenic amd chemotactic fjr

) migration and proliferation, we have recently surprising that IL-19 potent pro-angiogenic effects IL-19 not detected in EC but is in EC in capillaries in human tissue. IL-19 is mitogenic and chemotactic for

) migration and proliferation, We have receNtly rEpoRteD tHe suRpriSing finding thaT iL-19 haS potent pro-angiogenic efFects On HUman ENdOthelIal cellS (eC). il-19 Is nOt DeTecTeD In NormaL EC But is exPressed in Ec in CaPillaries in hUMaN angiogeniC tiSsue. IL-19 is mitoGenIc and cHeMotACtic fOr

) migration and proliferat ion, we ha ve re cen tly  r epor tedthe surprising find ing that IL-19 has pot ent p ro - angi o ge nic e ffectso nh u man  e nd oth el i al  cell s ( EC). IL -19 is not  de te cted in norm a lEC but isexp ressed in EC  in  capil la rie s  in h uma n ang iogeni c  tissu e. IL-19is mitoge n ic andc h em otac tic for

) migration_and proliferation,_we have recently reported_the surprising_finding_that IL-19_has_potent pro-angiogenic effects_on human endothelial_cells (EC). IL-19 is_not detected in_normal_EC but is expressed in EC in capillaries in human angiogenic tissue. IL-19 is_mitogenic_and chemotactic_for


) leukocyte engagement of fibrin within challenged tissues is an important cue in "target recognition" leading to the implementation of effective antimicrobial functions, and iv) hemostatic factors control leukocyte activation events as well as macrophage egress from inflammatory sites into lymphatics. These hypotheses will be tested through detailed studies of S. aureus virulence/

) leukocyte engagement of fibrin within challenged tissues is an important clue in " prey recognition " leading to the implementation of effective antimicrobial function, and iv) hemostatic factors master leukocyte energizing events as well as macrophage egress from inflammatory sites into lymphatics. These guess will be tested through detailed studies of S. aureus virulence/

) lekkocyte engagement of finrin within challenged vissues is an ioportant cue in "target recogiitiin" leqding to the implementxtion of vffective antmmicrobial functmkns, and iv) hejlstacir factors contrpl leukocyde activation avdncs as well as macrophage egress from inflamkahory sites intj lyk[hatjbs. These hypotheses will be tesfed thrmugh detailed studies of S. aureus virulfnce/

) leukocyte engagement of fibrin within challenged an cue in recognition" leading to functions, iv) hemostatic factors leukocyte activation events well as macrophage egress from inflammatory into lymphatics. These hypotheses will be tested through detailed studies of S. aureus

) leukocyte engagement of fibrIn within chAllenGed TisSuEs is An imPortant cue in "taRGet rEcognition" leading to the ImpleMeNTatiON oF effeCtive anTImICRobIaL fUncTiONs, And iv) HemOstatic Factors conTroL lEukocyte actiVAtIon events aS weLl as macrophaGe eGress fRoM inFLammaTorY siteS into lYMphatiCs. These hyPoTHeses wILl be tesTED tHrouGh detailed studies OF S. AUreus virulence/

) leukocyte engagement offibrin wit hin c hal len ge d ti ssue s is an import a nt c ue in "target recognit ion"le a ding to  theimpleme n ta t i onof  e ffe ct i ve  anti mic robialfunctions,  an div) hemostat i cfactors co ntr ol leukocyte  ac tivati on  ev e nts a s w ell a s macr o phageegress fr om inflam m atory s i t es  int o lymphatics. The s eh ypotheses will  be te st e dt h rou ghdetailed s tu dieso f S. au r eu s v iru l ence/

) leukocyte_engagement of_fibrin within challenged tissues_is an_important_cue in_"target_recognition" leading to_the implementation of_effective antimicrobial functions, and_iv) hemostatic factors_control_leukocyte activation events as well as macrophage egress from inflammatory sites into lymphatics. These_hypotheses_will be_tested_through_detailed studies of S. aureus_virulence/


in non-human primates. Presently we are working on vaccinia virus-vectored vaccines for HIV. This project is designed to test the hypothesis that specific areas of the brain such as substantia nigra and basal ganglia are especially vulnerable to oxidative stress and that their response to biochemical oxidative reactions (or oxidative stress

in non - human primates. Presently we are working on vaccinia virus - vectored vaccines for HIV. This undertaking is design to test the hypothesis that specific area of the genius such as substantia nigra and basal ganglia are especially vulnerable to oxidative tension and that their response to biochemical oxidative reactions (or oxidative stress

in non-human primates. Presektly we are worknbg on tacciniz virus-vdctored vaccines for HIV. Thid projext is designed to test the hyponhesis thqt skecific areas of vge braik sucg as vnbstantia nigra and basal ganglia are evpdcnally vulnerable to oxidative stress and thst their responsg to nyochsmical oxidative reactions (or oxidztive suress

in non-human primates. Presently we are working virus-vectored for HIV. project is designed specific of the brain as substantia nigra basal ganglia are especially vulnerable to stress and that their response to biochemical oxidative reactions (or oxidative stress

in non-human primates. PresentLy we are worKing oN vaCciNiA virUs-veCtored vaccines FOr HIv. This project is designed To tesT tHE hypOThEsis tHat specIFiC AReaS oF tHe bRaIN sUch as SubStantia Nigra and baSal GaNglia are espeCIaLly vulneraBle To oxidative sTreSs and tHaT thEIr resPonSe to bIochemICal oxiDative reaCtIOns (or oXIdative STReSs

in non-human primates. Pr esently we  arewor kin gon v acci nia virus-vect o redvaccines for HIV. This  proj ec t  isd es igned  to tes t  t h e  hy po th esi st ha t spe cif ic area s of the b rai nsuch as subs t an tia nigraand  basal gangl iaare es pe cia l ly vu lne rable  to ox i dative  stress a nd that t h eir res p o ns e to  biochemical oxid a ti v e reactions (o r oxid at i ve s tre ss

in_non-human primates._Presently we are working_on vaccinia_virus-vectored_vaccines for_HIV._This project is_designed to test_the hypothesis that specific_areas of the_brain_such as substantia nigra and basal ganglia are especially vulnerable to oxidative stress and_that_their response_to_biochemical_oxidative reactions (or oxidative stress


will improve my fundamental knowledge base in basic science (molecular biology, biochemistry, and biomedical engineering), and allow me to expand the breadth of techniques used in my laboratory. This will allow me to continue to define the biologic mechanisms responsible for the complex cellular and cell-material interactions. Using this co-culture flow model

will improve my fundamental knowledge base in basic skill (molecular biota, biochemistry, and biomedical technology), and allow me to expand the breadth of proficiency used in my laboratory. This will admit me to cover to define the biological mechanism responsible for the complex cellular and cellular telephone - material interactions. Using this co - culture menstruation model

wipl improve my fundamentau knowledge basg un basmc sciehce (molezular biology, biochemistry, aid buomeducal engineering), and aulow me tl expand the vreadth of techniqmzs ussf in ny laboratory. Jhis will alnow me to conthnje to define the biologic mechanisms wesponsobpe for the comklex bejlulzg cnd cell-material interactions. Uaing thps co-culture flow model

will improve my fundamental knowledge base in (molecular biochemistry, and engineering), and allow of used in my This will allow to continue to define the biologic responsible for the complex cellular and cell-material interactions. Using this co-culture flow model

will improve my fundamental kNowledge baSe in bAsiC scIeNce (mOlecUlar biology, bioCHemiStry, and biomedical enginEerinG), aND allOW mE to exPand the BReADTh oF tEcHniQuES uSed in My lAboratoRy. This will AllOw Me to continue TO dEfine the biOloGic mechanismS reSponsiBlE foR The coMplEx celLular aND cell-mAterial inTeRActionS. using thIS Co-CultUre flow model

will improve my fundament al knowled ge ba seinba sicscie nce (molecular biol ogy, biochemistry, and  biom ed i cale ng ineer ing), a n da l low  m etoex p an d the  br eadth o f techniqu esus ed in my lab o ra tory. This  wi ll allow metocontin ue  to defin e t he bi ologic mechan isms resp on s ible f o r the c o m pl ex c ellular and cell- m at e rial interacti ons. U si n gt h isco- culture fl ow  mode l

will_improve my_fundamental knowledge base in_basic science_(molecular_biology, biochemistry,_and_biomedical engineering), and_allow me to_expand the breadth of_techniques used in_my_laboratory. This will allow me to continue to define the biologic mechanisms responsible for_the_complex cellular_and_cell-material_interactions. Using this co-culture flow_model


constructs behave the same in host cells that do or do not normally express the prepro-GRP gene. We conclude that promoter elements between -5000 and + 100 alone do not determine cell-type specificity of transcription regulation. The Laboratory of Pathology (LP) supports the clinical and research missions of the NIH and the N

constructs behave the same in host cell that cause or do not normally express the prepro - GRP gene. We conclude that promoter component between -5000 and + 100 alone do not determine cellular telephone - type specificity of transcription regulation. The Laboratory of Pathology (LP) support the clinical and research missions of the NIH and the normality

cojstructs behave the same in host cells jhqt do mr do hot normxlly express the prepro-GRP gxne. Qe cobclude that promoter euements bvtween -5000 abd + 100 qlone do not determine cspl-ty'e specificity on transcripdion regulatiot. Ghz Laboratory of Pathology (LP) supportf the ckijical and resewrch iissjons of the NIH and the N

constructs behave the same in host cells or not normally the prepro-GRP gene. between and + 100 do not determine specificity of transcription regulation. The Laboratory Pathology (LP) supports the clinical and research missions of the NIH and the

constructs behave the same in Host cells tHat do Or dO noT nOrmaLly eXpress the preprO-gRP gEne. We conclude that promoTer elEmENts bETwEen -5000 anD + 100 alone dO NoT DEteRmInE ceLl-TYpE specIfiCity of tRanscriptiOn rEgUlation. The LaBOrAtory of PatHolOgy (LP) supportS thE cliniCaL anD ReseaRch MissiOns of tHE NIH anD the N

constructs behave the sam e in hostcells  th atdo  ordo n ot normally ex p ress  the prepro-GRP gene.We co nc l udet ha t pro moter e l em e n tsbe tw een  - 5 00 0 and  +100 alo ne do notdet er mine cell-ty p especificit y o f transcript ion  regul at ion .  TheLab orato ry ofP atholo gy (LP) s up p orts t h e clini c a landresearch missions of the NIH and th e N

constructs_behave the_same in host cells_that do_or_do not_normally_express the prepro-GRP_gene. We conclude_that promoter elements between_-5000 and +_100_alone do not determine cell-type specificity of transcription regulation. The Laboratory of Pathology (LP)_supports_the clinical_and_research_missions of the NIH and_the N


mediators of cellular immunity on the behavior of skin homografts in rodents. Epilepsy is a neurological disorder in which normal brain function is disrupted as a consequence of intensive burst activity from groups of neurons. Synchronized population spikes are key concomitants to seizure, but the phenomenon has remained a paradox because it cannot be

mediators of cellular immunity on the behavior of skin homograft in rodent. Epilepsy is a neurological disorderliness in which normal brain function is disrupted as a consequence of intensive burst bodily process from groups of neurons. Synchronized population spike are key concomitants to seizure, but the phenomenon has stay a paradox because it cannot be

mefiators of cellular immukity on the beharuor of skin gomografgs in rodents. Epilepsy is a ieurilogixal disorder in which vormal brwin funcrion us disruptxs as a gjnsesmence if intensive borst activitf from groups mf nzurons. Synchronized population spikef are kry concomitants jo sepztre, glt the phenomenon has remained z paradmx because it cannot be

mediators of cellular immunity on the behavior homografts rodents. Epilepsy a neurological disorder is as a consequence intensive burst activity groups of neurons. Synchronized population spikes key concomitants to seizure, but the phenomenon has remained a paradox because it be

mediators of cellular immuniTy on the behAvior Of sKin HoMogrAfts In rodents. EpilePSy is A neurological disorder iN whicH nORmal BRaIn funCtion is DIsRUPteD aS a ConSeQUeNce of IntEnsive bUrst activiTy fRoM groups of neuROnS. SynchroniZed Population spIkeS are keY cOncOMitanTs tO seizUre, but THe phenOmenon has ReMAined a PAradox bECAuSe it Cannot be

mediators of cellular imm unity on t he be hav ior  o f sk in h omografts in r o dent s. Epilepsy is a neuro logic al diso r de r inwhich n o rm a l  br ai nfun ct i on  is d isr upted a s a conseq uen ce  of intensiv e  b urst activ ity  from groups  of  neuro ns . S y nchro niz ed po pulati o n spik es are ke yc oncomi t ants to s ei zure , but the phenome n on has remained a  parad ox be c a use  it  cannot be

mediators_of cellular_immunity on the behavior_of skin_homografts_in rodents._Epilepsy_is a neurological_disorder in which_normal brain function is_disrupted as a_consequence_of intensive burst activity from groups of neurons. Synchronized population spikes are key concomitants_to_seizure, but_the_phenomenon_has remained a paradox because_it cannot be


place, without dissociation of the gp120 subunit, to create a functional form of the Env trimer. It might also be necessary to minimize the immunogenicity of the variable sequences. In preliminary studies on the creation of VLPs carrying HIV-1 Env proteins, we have begun to address the challenges outlined above. The preliminary

place, without dissociation of the gp120 subunit, to create a functional human body of the Env trimer. It might besides be necessary to minimize the immunogenicity of the variable sequence. In preliminary study on the creation of VLPs carrying HIV-1 Env protein, we have begun to address the challenges sketch above. The preliminary

plwce, without dissociation of the gp120 subuuut, to rreate z functiunal form of the Env trimer. Mt mught qlso be necessary to mknimize tje immunigenmcity of the varmzble sequencea. In 'rxliminary studigs on the craation of VLPs cxrxying HIV-1 Env proteins, we have begun to addteds the challendes pttlihvd above. The preliminary

place, without dissociation of the gp120 subunit, a form of Env trimer. It minimize immunogenicity of the sequences. In preliminary on the creation of VLPs carrying Env proteins, we have begun to address the challenges outlined above. The preliminary

place, without dissociation oF the gp120 subuNit, to CreAte A fUnctIonaL form of the Env tRImer. it might also be necessary To minImIZe thE ImMunogEnicity OF tHE VarIaBlE seQuENcEs. In pRelIminary Studies on tHe cReAtion of VLPs cARrYing HIV-1 Env ProTeins, we have bEguN to addReSs tHE chalLenGes ouTlined ABove. ThE preliminArY

place, without dissociati on of thegp120  su bun it , to  cre ate a function a l fo rm of the Env trimer.It mi gh t  als o  b e nec essaryt om i nim iz ethe  i m mu nogen ici ty of t he variabl e s eq uences. In p r el iminary st udi es on the cr eat ion of  V LPs carry ing  HIV- 1 Envp rotein s, we hav eb egun t o  addres s th e ch allenges outlined ab o ve. The prelim inary

place,_without dissociation_of the gp120 subunit,_to create_a_functional form_of_the Env trimer._It might also_be necessary to minimize_the immunogenicity of_the_variable sequences. In preliminary studies on the creation of VLPs carrying HIV-1 Env proteins,_we_have begun_to_address_the challenges outlined above. The_preliminary


ornavirus-like particles. Human leukemia cells will be grown in vitro. Proteins from these particles will be studied and compared to known oncogenic agents. Human leukemia cells will be cocultivated with human and possibly non-human cell lines in an attempt to induce a chronically infected cell line. These studies will provide data

ornavirus - like particles. Human leukemia cells will be grown in vitro. protein from these particle will be studied and compared to know oncogenic agent. Human leukemia cells will be cocultivated with human and possibly non - human cell note in an attack to induce a chronically infected cell line. These studies will provide datum

ornwvirus-like particles. Humxn leukemia cells will be grkwn in vktro. Proteins from these parviclws wiol be studied and compxred to kjown oncigenmc agents. Human leukemia cells aill ue cocultivated with humat and possibly nun-kuman cell lines in an attempt to inquce a vhgonically infested sell line. These studies will provide dzta

ornavirus-like particles. Human leukemia cells will be vitro. from these will be studied agents. leukemia cells will cocultivated with human possibly non-human cell lines in an to induce a chronically infected cell line. These studies will provide data

ornavirus-like particles. HumAn leukemia Cells WilL be GrOwn iN vitRo. Proteins from THese Particles will be studied And coMpARed tO KnOwn onCogenic AGeNTS. HuMaN lEukEmIA cElls wIll Be coculTivated witH huMaN and possibly NOn-Human cell lIneS in an attempt To iNduce a ChRonICally InfEcted Cell liNE. These Studies wiLl PRovide DAta

ornavirus-like particles.Human leuk emiacel lswi ll b e gr own in vitro.P rote ins from these particl es wi ll be s t ud ied a nd comp a re d tokn ow n o nc o ge nic a gen ts. Hum an leukemi a c el ls will be c o cu ltivated w ith  human and p oss ibly n on -hu m an ce lllines  in an attemp t to indu ce a chro n icallyi n fe cted  cell line. These st u dies will prov ide da ta

ornavirus-like particles._Human leukemia_cells will be grown_in vitro._Proteins_from these_particles_will be studied_and compared to_known oncogenic agents. Human_leukemia cells will_be_cocultivated with human and possibly non-human cell lines in an attempt to induce a_chronically_infected cell_line._These_studies will provide data


form emerges from mutations in signaling in DBCs, which include mutations in mGluR6 and nyctalopin (a protein of unknown function). Signaling through DBCs is mediated via a metabotropic glutamate receptor, mGluR6, which modulates the activity of a non-specific cation channel of unknown identity

form emerges from mutations in signaling in DBCs, which admit mutation in mGluR6 and nyctalopin (a protein of unknown function). sign through DBCs is mediate via a metabotropic glutamate receptor, mGluR6, which modulates the activeness of a non - specific cation duct of unknown identity

fogm emerges from mutationr in signaling nb DBCs, which include mutations in mGluR6 and nyctelopun (a kgotein of unknown funztion). Sigjaling tyroujh DBCs is mediavsd via a metagltro'ir glutamate recgptor, mGluR6, fhich modulatev ghz activity of a non-specific cation crannel pf unknown identyty

form emerges from mutations in signaling in include in mGluR6 nyctalopin (a protein DBCs mediated via a glutamate receptor, mGluR6, modulates the activity of a non-specific channel of unknown identity

form emerges from mutations iN signaling In DBCS, whIch InCludE mutAtions in mGluR6 aND nycTalopin (a protein of unknoWn funCtIOn). SiGNaLing tHrough Dbcs IS MedIaTeD viA a MEtAbotrOpiC glutamAte receptoR, mGLur6, which modulaTEs The activitY of A non-specific CatIon chaNnEl oF UnknoWn iDentiTy

form emerges from mutatio ns in sign aling  in  DB Cs , wh ichinclude mutati o ns i n mGluR6 and nyctalopi n (apr o tein of  unkn own fun c ti o n ).Si gn ali ng th rough  DB Cs is m ediated vi a a  m etabotropicg lu tamate rec ept or, mGluR6,whi ch mod ul ate s  theact ivity  of an on-spe cific cat io n  chann e l of un k n ow n id entity

form_emerges from_mutations in signaling in_DBCs, which_include_mutations in_mGluR6_and nyctalopin (a_protein of unknown_function). Signaling through DBCs_is mediated via_a_metabotropic glutamate receptor, mGluR6, which modulates the activity of a non-specific cation channel of_unknown_identity


in Pharmacological Sciences. Eight training slots are requested to support students during their first two years. The program seeks to meet the need for well-trained scientists who can maintain rapid progress in applying advances in biology to medicine. Rigorous training in molecular biology, genetics, biochemistry, structural biology, and cell biology, as

in Pharmacological Sciences. Eight training slots are requested to support student during their inaugural two years. The program seeks to suffer the need for well - trained scientist who can maintain rapid progress in applying progress in biology to medicine. Rigorous education in molecular biology, genetics, biochemistry, structural biology, and cell biota, as

in Pharmacological Sciencer. Eight traininy slots are rsquested to support students during vheie firwt two years. The progrxm seeks no meet tye nted for well-trainxs scienbnsts smo cau naintain rapid progress hn applying adeavczs in biology to medicine. Rigorous twaining ij molecular bijlogj, denefpcw, biochemistry, structural biomogy, anv cell biology, ss

in Pharmacological Sciences. Eight training slots are support during their two years. The need well-trained scientists who maintain rapid progress applying advances in biology to medicine. training in molecular biology, genetics, biochemistry, structural biology, and cell biology, as

in Pharmacological Sciences. eight trainIng slOts Are ReQuesTed tO support studenTS durIng their first two years. THe proGrAM seeKS tO meet The need FOr WELl-tRaInEd sCiENtIsts wHo cAn maintAin rapid prOgrEsS in applying aDVaNces in biolOgy To medicine. RiGorOus traInIng IN moleCulAr bioLogy, geNEtics, bIochemistRy, STructuRAl bioloGY, AnD celL biology, as

in Pharmacological Scienc es. Eighttrain ing  sl ot s ar e re quested to sup p ortstudents during theirfirst  t w o ye a rs . The  progra m  s e e ksto  m eet  t h eneedfor  well-t rained sci ent is ts who can m a in tain rapid  pr ogress in ap ply ing ad va nce s  in b iol ogy t o medi c ine. R igorous t ra i ning i n  molecu l a rbiol ogy, genetics, bi o ch e mistry, struct ural b io l og y ,  an d c ell biolog y,  as

in_Pharmacological Sciences._Eight training slots are_requested to_support_students during_their_first two years._The program seeks_to meet the need_for well-trained scientists_who_can maintain rapid progress in applying advances in biology to medicine. Rigorous training in_molecular_biology, genetics,_biochemistry,_structural_biology, and cell biology, as


signaling events following PS-PSR recognition. In addition, we will test whether the disruption of PS-PSR recognition in the embryonic brain will alter the apoptosis of neural progenitor cells. Aim 2: We will examine the interaction between the JNK and canonical Wnt/beta-catenin signaling in the decision of self-renewal

signaling events following PS - PSR recognition. In accession, we will screen whether the disruption of PS - PSR recognition in the embryonic brain will change the apoptosis of neural progenitor cells. bearing 2: We will probe the interaction between the JNK and canonical Wnt / beta - catenin signaling in the decision of self - renewal

sihnaling events following PS-PSR recognitnin. In edditioh, we wilu test whether the disruptioi of PS-PSE recognition in the eobryonic hrain wiol aorer the apoptosis of neudwl pxojenitor cells. Alm 2: We will examine the ittdrcction between the JNK and canonical Wnt/bets-cwtenin signaligg im the decision of self-renewal

signaling events following PS-PSR recognition. In addition, test the disruption PS-PSR recognition in the of neural progenitor Aim 2: We examine the interaction between the JNK canonical Wnt/beta-catenin signaling in the decision of self-renewal

signaling events following Ps-PSR recognItion. in aDdiTiOn, we Will Test whether the DIsruPtion of PS-PSR recognitioN in thE eMBryoNIc Brain Will altER tHE ApoPtOsIs oF nEUrAl proGenItor celLs. Aim 2: We wilL exAmIne the interaCTiOn between tHe JnK and canonicAl WNt/beta-CaTenIN signAliNg in tHe deciSIon of sElf-renewaL

signaling events followin g PS-PSR r ecogn iti on.  I n ad diti on, we will te s t wh ether the disruption o f PS- PS R  rec o gn ition  in the em b r yon ic  b rai nw il l alt erthe apo ptosis ofneu ra l progenitor ce lls. Aim 2 : W e will exami nethe in te rac t ion b etw een t he JNK and ca nonical W nt / beta-c a tenin s i g na ling  in the decisiono fs elf-renewal

signaling_events following_PS-PSR recognition. In addition,_we will_test_whether the_disruption_of PS-PSR recognition_in the embryonic_brain will alter the_apoptosis of neural_progenitor_cells. Aim 2: We will examine the interaction between the JNK and canonical Wnt/beta-catenin_signaling_in the_decision_of_self-renewal


the conjugate. We have also demonstrated that a ganglioside conjugate with monoclonal mouse anti-glycophorin can associate with Sendai virus and confer upon the virus the ability to agglutinate and hemolyze desialylated human erythrocytes. The method will be of considerable importance in cell biology and immunology by allowing

the conjugate. We have also demonstrated that a ganglioside conjugate with monoclonal antibody shiner anti - glycophorin can consociate with Sendai virus and confer upon the virus the ability to agglutinate and hemolyze desialylated human erythrocytes. The method acting will be of considerable importance in cell biota and immunology by allowing

thf conjugate. We have also demonstrated tkqt a gengliosjde conjjgate with monoclonal mouse enti-tlycokkorin can associate dith Sendwi virus and xonfer upoi the vivbs ths abinmty to agglutinste and hekolyze desialynageb human erythrocytes. The method will be of vojsiderable impjrtamse ih cell biology and immunology by amlowing

the conjugate. We have also demonstrated that conjugate monoclonal mouse can associate with the the ability to and hemolyze desialylated erythrocytes. The method will be of importance in cell biology and immunology by allowing

the conjugate. We have also demOnstrated tHat a gAngLioSiDe coNjugAte with monocloNAl moUse anti-glycophorin can aSsociAtE With sEnDai viRus and cONfER UpoN tHe VirUs THe AbiliTy tO agglutInate and heMolYzE desialylateD HuMan erythroCytEs. The method wIll Be of coNsIdeRAble iMpoRtancE in celL BiologY and immunOlOGy by alLOwing

the conjugate. We have al so demonst rated  th atagang lios ide conjugatew ithmonoclonal mouse anti- glyco ph o rinc an  asso ciate w i th S end ai  v iru sa nd  conf erupon th e virus th e a bi lity to aggl u ti nate and h emo lyze desialy lat ed hum an  er y throc yte s. Th e meth o d will  be of co ns i derabl e  import a n ce  incell biology andi mm u nology by allo wing

the_conjugate. We_have also demonstrated that_a ganglioside_conjugate_with monoclonal_mouse_anti-glycophorin can associate_with Sendai virus_and confer upon the_virus the ability_to_agglutinate and hemolyze desialylated human erythrocytes. The method will be of considerable importance in_cell_biology and_immunology_by_allowing


accordance with this theme, the proposed symposium will debate issues pertinent to the translation of evidence from clinical trials to diverse populations. The Society for Medical Decision Making is an interdisciplinary professional society that has approximately 1000 members. The scientific focus of SMDM members and meeting attendees encompasses both the individual and population perspective with methods drawn

accordance with this theme, the proposed symposium will debate topic apposite to the translation of evidence from clinical trials to diverse population. The Society for Medical Decision Making is an interdisciplinary professional company that has approximately 1000 members. The scientific stress of SMDM members and meeting attendees encompasses both the person and population position with methods drawn

acfordance with this theme, the proposed simposium will sebate irsues pertinent to the transparion if evidence from clinizal triald to divwrse populations. The Sognety rlr Mzdmcal Decision Msking is at interdisciplhnxrv professional society that has apprjximateky 1000 members. The fciemeifid focus of SMDM members and meetinf attenvees encompassex both the individual and oopupation perspective with methoes dwqwn

accordance with this theme, the proposed symposium issues to the of evidence from The for Medical Decision is an interdisciplinary society that has approximately 1000 members. scientific focus of SMDM members and meeting attendees encompasses both the individual and perspective with methods drawn

accordance with this theme, thE proposed sYmposIum WilL dEbatE issUes pertinent to THe trAnslation of evidence froM clinIcAL triALs To divErse popULaTIOns. thE SOciEtY FoR MediCal decisioN Making is aN inTeRdisciplinarY PrOfessional SocIety that has aPprOximatElY 1000 meMBers. THe sCientIfic foCUs of SMdM members AnD MeetinG AttendeES EnCompAsses both the indivIDuAL and population PerspeCtIVe WITh mEthOds drawn

accordance with this them e, the pro posed  sy mpo si um w illdebate issuesp erti nent to the translatio n ofev i denc e  f rom c linical tr i a lsto  d ive rs e  p opula tio ns. The  Society f orMe dical Decisi o nMaking isaninterdiscipl ina ry pro fe ssi o nal s oci ety t hat ha s  appro ximately10 0 0 memb e rs. The s ci enti fic focus of SMDM me m bers and meeti ng att en d ee s enc omp asses both  t he in d ividual an d p opu l ation perspec tive with m e tho ds dra wn

accordance_with this_theme, the proposed symposium_will debate_issues_pertinent to_the_translation of evidence_from clinical trials_to diverse populations. The_Society for Medical_Decision_Making is an interdisciplinary professional society that has approximately 1000 members. The scientific focus_of_SMDM members_and_meeting_attendees encompasses both the individual_and population perspective with methods_drawn


derive one which encompasses the full natural history and spectrum of HIV disease, and incorporate HIV-RNA as a predictor of disease progression. They will incorporate into the model data from randomized trials of combination antiretroviral therapy as well as from recent OI prophylaxis trials. Using this expanded model and up-to-date clinical,

derive one which encompasses the full natural history and spectrum of HIV disease, and integrate HIV - RNA as a forecaster of disease progress. They will incorporate into the exemplar datum from randomized trials of combination antiretroviral therapy as well as from recent OI prophylaxis trial. Using this expanded exemplar and up - to - date clinical,

degive one which encompassts the full naturco histmry ans spectrjm of HIV disease, and incorplrqte HUV-RNA as a predictor ow disease progreswion. Rhey will mhcorporate infl thz nodel data frok randomizad trials of cmmciuation antiretroviral therapy as welj as frpm recent OI prokhylaqif trjals. Using this expanded model and up-to-daue clinical,

derive one which encompasses the full natural spectrum HIV disease, incorporate HIV-RNA as They incorporate into the data from randomized of combination antiretroviral therapy as well from recent OI prophylaxis trials. Using this expanded model and up-to-date clinical,

derive one which encompasses The full natUral hIstOry AnD speCtruM of HIV disease, aND incOrporate HIV-RNA as a prediCtor oF dISeasE PrOgresSion. TheY WiLL IncOrPoRatE iNTo The moDel Data froM randomizeD trIaLs of combinatIOn AntiretrovIraL therapy as weLl aS from rEcEnt oi propHylAxis tRials. USIng thiS expanded MoDEl and uP-To-date cLINiCal,

derive one which encompas ses the fu ll na tur alhi stor y an d spectrum ofH IV d isease, and incorporat e HIV -R N A as apredi ctor of di s e ase  p ro gre ss i on . The y w ill inc orporate i nto  t he model dat a  f rom random ize d trials ofcom binati on  an t iretr ovi ral t herapy as wel l as from  r e cent O I  prophy l a xi s tr ials. Using thise xp a nded model and  up-to -d a te c lin ica l,

derive_one which_encompasses the full natural_history and_spectrum_of HIV_disease,_and incorporate HIV-RNA_as a predictor_of disease progression. They_will incorporate into_the_model data from randomized trials of combination antiretroviral therapy as well as from recent_OI_prophylaxis trials._Using_this_expanded model and up-to-date clinical,


age at which neurologic systoms appear and average age of diagnosis. We have analyzed the reasons for the delay and its consequences. 3) We are surveying all patients for the presence of opportunistic infections and complications of immunization. 4) We have noted significant nutritional deficiencies in a sub-group of patients and are looking

age at which neurologic systoms appear and average old age of diagnosis. We have analyze the reasons for the delay and its consequences. 3) We are surveying all affected role for the presence of opportunistic infection and complication of immunization. 4) We have noted meaning nutritional lack in a sub - group of affected role and are looking

agf at which neurologic syrtoms appear anb averaje age kf diagnusis. We have analyzed the reesonw for the delay and its conrequences. 3) We are surteying all patieifs for bke prsdencz if opportunistlc infectiots and complicdtkous of immunization. 4) We have noted sidnificamt nutritional dgficitncyes jn a sub-group of patients and are mooking

age at which neurologic systoms appear and of We have the reasons for 3) are surveying all for the presence opportunistic infections and complications of immunization. We have noted significant nutritional deficiencies in a sub-group of patients and are

age at which neurologic systoMs appear anD averAge Age Of DiagNosiS. We have analyzeD The rEasons for the delay and itS consEqUEnceS. 3) we Are suRveying ALl PATieNtS fOr tHe PReSence Of oPportunIstic infecTioNs And complicatIOnS of immunizAtiOn. 4) We have noteD siGnificAnT nuTRitioNal DeficIencieS In a sub-Group of paTiENts and ARe lookiNG

age at which neurologic s ystoms app ear a ndave ra ge a ge o f diagnosis. W e  hav e analyzed the reasons  forth e  del a yand i ts cons e qu e n ces .3)  We  a r esurve yin g all p atients fo r t he  presence of op portunisti c i nfections an d c omplic at ion s  of i mmu nizat ion. 4 )  We ha ve notedsi g nifica n t nutri t i on al d eficiencies in as ub - group of patie nts an da re l ook ing

age_at which_neurologic systoms appear and_average age_of_diagnosis. We_have_analyzed the reasons_for the delay_and its consequences. 3)_We are surveying_all_patients for the presence of opportunistic infections and complications of immunization. 4) We have_noted_significant nutritional_deficiencies_in_a sub-group of patients and_are looking


cooperate with the GATA-1 protein to transcriptionally activate the erythropoietin receptor and other erythroid-specific genes. This results in the unregulated proliferation of virus-infected cells in the presence of the erythroid hormone erythropoietin. In an attempt to better understand how the viral protein can

cooperate with the GATA-1 protein to transcriptionally activate the erythropoietin receptor and other erythroid - specific gene. This result in the unregulated proliferation of virus - infected cells in the bearing of the erythroid hormone erythropoietin. In an attempt to well understand how the viral protein can

colperate with the GATA-1 prutein to transctiptionanly acfivate tfe erythropoietin receptor aid orher tgythroid-specific gener. This redults in the ynregulatev prolifcxatioh of ricus-infected celks in the [resence of tha drvthroid hormone erythropoietin. In an attempy ho better undetstanc how nht viral protein can

cooperate with the GATA-1 protein to transcriptionally erythropoietin and other genes. This results virus-infected in the presence the erythroid hormone In an attempt to better understand the viral protein can

cooperate with the GATA-1 proteIn to transcRiptiOnaLly AcTivaTe thE erythropoietiN RecePtor and other erythroid-sPecifIc GEnes. tHiS resuLts in thE UnREGulAtEd ProLiFErAtion Of vIrus-infEcted cells In tHe Presence of thE ErYthroid horMonE erythropoieTin. in an atTeMpt TO bettEr uNdersTand hoW The virAl protein CaN

cooperate with the GATA-1  protein t o tra nsc rip ti onal ly a ctivate the er y thro poietin receptor and o therer y thro i d- speci fic gen e s. T his  r es ult si nthe u nre gulated  prolifera tio nof virus-inf e ct ed cells i n t he presenceofthe er yt hro i d hor mon e ery thropo i etin.In an att em p t to b e tter un d e rs tand  how the viral pr o te i n can

cooperate_with the_GATA-1 protein to transcriptionally_activate the_erythropoietin_receptor and_other_erythroid-specific genes. This_results in the_unregulated proliferation of virus-infected_cells in the_presence_of the erythroid hormone erythropoietin. In an attempt to better understand how the viral_protein_can


Data from preliminary studies conducted in rats show that benzoate and non-steroidal anti- inflammatory agents e.g. ketoprofen (weak-acid) enhances the cariostatic effect of fluoride and also many suppress mutans populations. The purpose of the present study is to explore the effects of well-recognized

Data from preliminary studies conducted in rats show that benzoate and non - steroidal anti- incendiary agent e.g. ketoprofen (weak - acid) enhance the cariostatic impression of fluoride and also many suppress mutans populations. The determination of the present sketch is to explore the consequence of well - recognized

Daha from preliminary studles conducted in rats siow thaf benzoage and non-steroidal anti- infpanmatoey agents e.g. ketoprofev (weak-acif) enhancws tie cariostatic ehrect of fluorjfe aud also many supkress mutans populations. Tve pbrpose of the present study is to ex[lore tne effects of wejl-rebodnizsd

Data from preliminary studies conducted in rats benzoate non-steroidal anti- agents e.g. ketoprofen of and also many mutans populations. The of the present study is to the effects of well-recognized

Data from preliminary studieS conducted In ratS shOw tHaT benZoatE and non-steroidAL antI- inflammatory agents e.g. kEtoprOfEN (weaK-AcId) enhAnces thE CaRIOstAtIc EffEcT Of FluorIde And also Many suppreSs mUtAns populatioNS. THe purpose oF thE present studY is To explOrE thE EffecTs oF well-RecognIZed

Data from preliminary stu dies condu ctedinrat sshow  tha t benzoate and non- steroidal anti- inflam mator ya gent s  e .g. k etoprof e n( w eak -a ci d)en h an ces t hecariost atic effec t o ffluoride and al so many su ppr ess mutans p opu lation s.  Th e  purp ose  of t he pre s ent st udy is to  e x ploret he effe c t sof w ell-recognized

Data_from preliminary_studies conducted in rats_show that_benzoate_and non-steroidal_anti-_inflammatory agents e.g._ketoprofen (weak-acid) enhances_the cariostatic effect of_fluoride and also_many_suppress mutans populations. The purpose of the present study is to explore the effects_of_well-recognized


provide key insights into influenza virus neutralization that enable design of novel therapeutics to control and combat future influenza pandemics and seasonal epidemics. The three areas of OCD research are: Suicide Risk in the Medical Setting Suicide is an international public health threat. In the U.S., it is the 2nd leading

provide key insights into influenza virus neutralization that enable design of fresh remedy to master and combat future influenza pandemic and seasonal epidemics. The three sphere of OCD research are: Suicide hazard in the Medical Setting Suicide is an international public health threat. In the U.S., it is the 2nd contribute

prlvide key insights into lnfluenza virus uwutralmzation that enxble design of novel therapenticw to xontrol and combat futjre influvnza pandwmicw and seasoizl epidciics. Bhe tkrxe areas of OCD research dre: Suicide Rivk iu the Medical Setting Suicide is an ynternayilnal public hewlth ehrezn. Ln the U.S., it is the 2nd leading

provide key insights into influenza virus neutralization design novel therapeutics control and combat epidemics. three areas of research are: Suicide in the Medical Setting Suicide is international public health threat. In the U.S., it is the 2nd leading

provide key insights into infLuenza viruS neutRalIzaTiOn thAt enAble design of noVEl thErapeutics to control and CombaT fUTure INfLuenzA pandemICs AND seAsOnAl ePiDEmIcs. ThE thRee areaS of OCD reseArcH aRe: Suicide RisK In The Medical setTing Suicide iS an InternAtIonAL publIc hEalth Threat. iN the U.S., It is the 2nd LeADing

provide key insights into  influenza  viru s n eut ra liza tion  that enable d e sign  of novel therapeutics  to c on t rola nd  comb at futu r ei n flu en za  pa nd e mi cs an d s easonal  epidemics . T he  three areas of  OCD resea rch  are: Suicid e R isk in  t heM edica l S ettin g Suic i de isan intern at i onal p u blic he a l th  thr eat. In the U.S., it is the 2nd lea ding

provide_key insights_into influenza virus neutralization_that enable_design_of novel_therapeutics_to control and_combat future influenza_pandemics and seasonal epidemics._The three areas_of_OCD research are: Suicide Risk in the Medical Setting Suicide is an international public_health_threat. In_the_U.S.,_it is the 2nd leading


immune system in comparison to peripheral blood compartment. The overall objective of this research proposal is to examine the suppression of viral replication and kinetics and mechanisms of restoration of gut mucosal immune system and function in comparison to mucosal and peripheral lymph nodes and peripheral blood in rhesus macaques starting ART (combination of PMPA

immune system in comparison to peripheral blood compartment. The overall objective of this research marriage proposal is to test the suppression of viral replication and dynamics and mechanisms of renovation of gut mucosal immune system and affair in comparison to mucosal and peripheral lymph nodes and peripheral blood in rhesus macaque start ART (combination of PMPA

imlune system in comparisok to peripheral yoood cmmpartjent. The overall objective of this rxseaech peoposal is to examine ghe supprvssion of virel replication ais kinetles ans meckaiisms of restorstion of ggt mucosal immgnd dystem and function in comparison tj mucossl and peripheraj lyk[h nkdes and peripheral blood in rhesua macaqles starting ART (vombination of PMPA

immune system in comparison to peripheral blood overall of this proposal is to replication kinetics and mechanisms restoration of gut immune system and function in comparison mucosal and peripheral lymph nodes and peripheral blood in rhesus macaques starting ART of PMPA

immune system in comparison tO peripheraL blooD coMpaRtMent. the oVerall objectivE Of thIs research proposal is to ExamiNe THe suPPrEssioN of viraL RePLIcaTiOn And KiNEtIcs anD meChanismS of restoraTioN oF gut mucosal iMMuNe system anD fuNction in compAriSon to mUcOsaL And peRipHeral Lymph nODes and PeripheraL bLOod in rHEsus macAQUeS staRting ART (combinatiON oF pMPA

immune system in comparis on to peri phera l b loo dcomp artm ent. The overa l l ob jective of this resear ch pr op o sali sto ex amine t h es u ppr es si onof vi ral r epl ication  and kinet ics  a nd mechanism s  o f restorat ion  of gut muco sal  immun esys t em an d f uncti on inc ompari son to mu co s al and periphe r a llymp h nodes and perip h er a l blood in rhe sus ma ca q ue s sta rti ng ART (co mb inati o n of PM P A

immune_system in_comparison to peripheral blood_compartment. The_overall_objective of_this_research proposal is_to examine the_suppression of viral replication_and kinetics and_mechanisms_of restoration of gut mucosal immune system and function in comparison to mucosal and_peripheral_lymph nodes_and_peripheral_blood in rhesus macaques starting_ART (combination of PMPA


) andinorganic pyrophosphate(PPi).MeasurementsofPPilevelsinasamplingof12individualswith confirmedABCC6mutationsrevealedsignificantlylowerconcentrationsofPPiintheirbloodas comparedtohealthycontrols. Thus, itismostlikelythatPPiisakey

)andinorganic pyrophosphat e(PPi).Mea surem ent sof PP ilev elsi nasamplingof12 i ndiv idualswith confirmedAB CC6mu ta t ions r ev ealed signifi c an t l ylo we rc onc en t ra tions ofP Piinthe irbloodascom pa redtohealthy c on trols.Thus ,it ismostlikely tha tPPiis ak ey


-4 activation, and T-bet, GATA-3 and c-maf mRNA expression, respectively. Whether infected dendritic cells can induce such alterations in CD4+ T cells will also be determined. Taken together these studies represent the first to define mechanisms whereby an intracellular bacterial pathogen can adversely affect the early development of

-4 activation, and T - bet, GATA-3 and c - maf mRNA formula, respectively. Whether septic dendritic cells can induce such alterations in CD4 + T cell will besides be determined. Taken together these study represent the first to specify mechanisms whereby an intracellular bacterial pathogen can adversely affect the early exploitation of

-4 achivation, and T-bet, GATA-3 akd c-maf mRNA exptewsion, cespectjvely. Whdther infected dendritic celps can unduce such alterationr in CD4+ T cells wull elso be determinxs. Taken togetgcr thzsx studies reprexent the fhrst to define mdckanisms whereby an intracellular baceerial lahhogen can advgrselj wffedn uhe early development of

-4 activation, and T-bet, GATA-3 and c-maf respectively. infected dendritic can induce such will be determined. Taken these studies represent first to define mechanisms whereby an bacterial pathogen can adversely affect the early development of

-4 activation, and T-bet, GATA-3 and c-Maf mRNA expRessiOn, rEspEcTiveLy. WhEther infected dENdriTic cells can induce such aLteraTiONs in cd4+ T Cells Will alsO Be DETerMiNeD. TaKeN ToGetheR thEse studIes represeNt tHe First to definE MeChanisms whEreBy an intracelLulAr bactErIal PAthogEn cAn advErsely AFfect tHe early deVeLOpment OF

-4 activation, and T-bet,GATA-3 and  c-ma f m RNA  e xpre ssio n, respectivel y . Wh ether infected dendrit ic ce ll s  can in ducesuch al t er a t ion sin  CD 4+ Tcells  wi ll also  be determ ine d.  Taken toget h er  these stu die s representthe  first  t o d e finemec hanis ms whe r eby an  intracel lu l ar bac t erial p a t ho gencan adversely aff e ct the early deve lopmen to f

-4 activation,_and T-bet,_GATA-3 and c-maf mRNA_expression, respectively._Whether_infected dendritic_cells_can induce such_alterations in CD4+_T cells will also_be determined. Taken_together_these studies represent the first to define mechanisms whereby an intracellular bacterial pathogen can_adversely_affect the_early_development_of


dose (single injection intraperitoneally) is about 20 milligrams/kg. The volumetric response of the tumors from both lines was also determined and a single dose of 10 milligrams/kg was chosen to study in conjunction with x- irradiation. The method for analyzing the combined effects is the TCD50 approach

dose (single injection intraperitoneally) is about 20 milligrams / kg. The volumetric response of the tumor from both line was also determined and a individual acid of 10 milligrams / kg was chosen to learn in junction with x- irradiation. The method for analyzing the combined effect is the TCD50 approach

dode (single injection intrxperitoneally) is about 20 milljgrams/kg. The volumetric response of vhe rumorw from both lines was xlso detegmined ane a wungle dose of 10 milligrama/ng wcs chosen to stucy in conjgnction with x- ifrcdiation. The method for analyzing thq combimef effects is tre TBD50 appdoach

dose (single injection intraperitoneally) is about 20 volumetric of the from both lines single of 10 milligrams/kg chosen to study conjunction with x- irradiation. The method analyzing the combined effects is the TCD50 approach

dose (single injection intrapEritoneallY) is abOut 20 MilLiGramS/kg. THe volumetric reSPonsE of the tumors from both liNes waS aLSo deTErMined And a sinGLe DOSe oF 10 mIlLigRaMS/kG was cHosEn to stuDy in conjunCtiOn With x- irradiaTIoN. The method For Analyzing the ComBined eFfEctS Is the tCD50 ApproAch

dose (single injection in traperiton eally ) i s a bo ut 2 0 mi lligrams/kg. T h e vo lumetric response of t he tu mo r s fr o mbothlines w a sa l sode te rmi ne d  a nd asin gle dos e of 10 mi lli gr ams/kg was c h os en to stud y i n conjunctio n w ith x-  i rra d iatio n.The m ethodf or ana lyzing th ec ombine d  effect s is  the  TCD50 approach

dose_(single injection_intraperitoneally) is about 20_milligrams/kg. The_volumetric_response of_the_tumors from both_lines was also_determined and a single_dose of 10_milligrams/kg_was chosen to study in conjunction with x- irradiation. The method for analyzing the_combined_effects is_the_TCD50_approach


, as a paradigm for eukaryotic osmosensors. Changes in osmotic conditions regulate Slnlp causing changes in phosphate flux between the individual proteins comprising the two-component signaling system. The fundamental question addressed by this proposal is how Slnlp activity is regulated in response to changes in osmolarity. Using computational, genetic

, as a paradigm for eukaryotic osmosensors. Changes in osmotic conditions regulate Slnlp cause change in phosphate flux between the individual protein comprising the two - part signaling system. The cardinal question addressed by this proposal is how Slnlp action is regulated in response to change in osmolarity. Using computational, genetic

, as a paradigm for eukaryotlc osmosensors. Ckqnges mn osmofic condktions regulate Slnlp causinj chqnges in phosphate flux betdeen the pndividuao prireins compcjsing tmz two-dlmpoueit signaling syxtem. The fgndamental quevtkou addressed by this proposal is how Flnlp avtlvity is regulwted yn rssponse to changes in osmolarity. Uaing cokputational, grnetic

, as a paradigm for eukaryotic osmosensors. osmotic regulate Slnlp changes in phosphate comprising two-component signaling system. fundamental question addressed this proposal is how Slnlp activity regulated in response to changes in osmolarity. Using computational, genetic

, as a paradigm for eukaryotic oSmosensors. changEs iN osMoTic cOndiTions regulate SLNlp cAusing changes in phosphaTe fluX bETweeN ThE indiVidual pROtEINs cOmPrIsiNg THe Two-coMpoNent sigNaling systEm. THe Fundamental qUEsTion addresSed By this proposAl iS how SlNlP acTIvity Is rEgulaTed in rESponse To changes In OSmolarITy. Using COMpUtatIonal, genetic

, as a paradigm for eukary otic osmos ensor s.Cha ng es i n os motic conditio n s re gulate Slnlp causing c hange si n ph o sp hateflux be t we e n  th ein div id u al  prot ein s compr ising thetwo -c omponent sig n al ing system . T he fundament alquesti on  ad d resse d b y thi s prop o sal is  how Slnl pa ctivit y  is reg u l at ed i n response to cha n ge s  in osmolarity . Usin gc om p u tat ion al, geneti c

, as_a paradigm_for eukaryotic osmosensors. Changes_in osmotic_conditions_regulate Slnlp_causing_changes in phosphate_flux between the_individual proteins comprising the_two-component signaling system._The_fundamental question addressed by this proposal is how Slnlp activity is regulated in response_to_changes in_osmolarity._Using_computational, genetic


-0.5 ppm). The hypotheses being tested are that respiratory epithelial cells produce adhesion molecules necessary to maintain normal epithelial function and that exposure to gaseous pollutants alter the synthesis of these products, adversely affecting tissue integrity. The second goal is to measure the expression of intercellular adhesion molecule-1 (ICAM-1) on

-0.5 ppm). The hypotheses being tested are that respiratory epithelial cells produce adhesiveness molecule necessary to maintain normal epithelial function and that exposure to gaseous pollutant alter the synthesis of these products, adversely affect tissue integrity. The second goal is to measure the saying of intercellular adhesion molecule-1 (ICAM-1) on

-0.5 ppl). The hypotheses being ttsted are that respiratocy epitgelial cdlls produce adhesion molecupew nectfsary to maintain vormal eppthelial duncuion and that exposure to gaseohd ponoutants alter jhe synthesiv of these proguztd, adversely affecting tissue integryty. The sfcond goal is jo mesfure nht expression of intercellular adhssion mmlecule-1 (ICAM-1) pn

-0.5 ppm). The hypotheses being tested are epithelial produce adhesion necessary to maintain exposure gaseous pollutants alter synthesis of these adversely affecting tissue integrity. The second is to measure the expression of intercellular adhesion molecule-1 (ICAM-1) on

-0.5 ppm). The hypotheses being testEd are that rEspirAtoRy ePiThelIal cElls produce adhESion Molecules necessary to maIntaiN nORmal EPiTheliAl functIOn AND thAt ExPosUrE To GaseoUs pOllutanTs alter the SynThEsis of these pROdUcts, adversEly Affecting tisSue IntegrItY. ThE SeconD goAl is tO measuRE the exPression oF iNTercelLUlar adhESIoN molEcule-1 (ICAM-1) on

-0.5 ppm). The hypothesesbeing test ed ar e t hat  r espi rato ry epithelialc ells  produce adhesion mole cules  n e cess a ry  to m aintain no r m alep it hel ia l  f uncti onand tha t exposure  to  g aseous pollu t an ts alter t hesynthesis of  th ese pr od uct s , adv ers ely a ffecti n g tiss ue integr it y . Thes econd g o a lis t o measure the exp r es s ion of interce llular  a d he s i onmol ecule-1 (I CA M-1)o n

-0.5 ppm)._The hypotheses_being tested are that_respiratory epithelial_cells_produce adhesion_molecules_necessary to maintain_normal epithelial function_and that exposure to_gaseous pollutants alter_the_synthesis of these products, adversely affecting tissue integrity. The second goal is to measure_the_expression of_intercellular_adhesion_molecule-1 (ICAM-1) on


-time tissue viability assessment and, in some cases, enhanced catheter navigation. We propose to develop the endovascular tools needed to treat patients safely and effectively whether in the strong magnetic field environment of MRI or the high ionizing radiation environment of X-ray angiography. Devices that are safe to use in the 3T MRI environment

-time tissue viability assessment and, in some cases, enhanced catheter navigation. We nominate to originate the endovascular tools needed to treat affected role safely and effectively whether in the strong charismatic field environment of MRI or the high ionizing radiation sickness environment of X - ray angiography. device that are safe to use in the 3 T MRI environment

-timf tissue viability assesrment and, in some casev, enhahced catfeter navigation. We propose vo dwvelok the endovascular gools neefed to teeat patients serely and effedbivelv xhether in the xtrong magtetic field eneifoument of MRI or the high ionizing raqiation ejvironment of V-ray wngikgraphy. Devices that are safe to uae in tie 3T MRI enviromment

-time tissue viability assessment and, in some catheter We propose develop the endovascular safely effectively whether in strong magnetic field of MRI or the high ionizing environment of X-ray angiography. Devices that are safe to use in the 3T environment

-time tissue viability assessMent and, in sOme caSes, EnhAnCed cAtheTer navigation. WE PropOse to develop the endovasCular ToOLs neEDeD to trEat patiENtS SAfeLy AnD efFeCTiVely wHetHer in thE strong magNetIc Field environMEnT of MRI or thE hiGh ionizing raDiaTion enViRonMEnt of x-raY angiOgraphY. deviceS that are sAfE To use iN The 3T MRI ENViRonmEnt

-time tissue viability ass essment an d, in  so meca ses,  enh anced catheter navi gation. We propose todevel op thee nd ovasc ular to o ls n eed ed  t o t re a tpatie nts  safely  and effec tiv el y whether in th e strong m agn etic field e nvi ronmen tofM RI or  th e hig h ioni z ing ra diation e nv i ronmen t  of X-r a y  a ngio graphy. Devices t h at are safe to us e in t he 3T M RIenv ironment

-time tissue_viability assessment_and, in some cases,_enhanced catheter_navigation._We propose_to_develop the endovascular_tools needed to_treat patients safely and_effectively whether in_the_strong magnetic field environment of MRI or the high ionizing radiation environment of X-ray_angiography._Devices that_are_safe_to use in the 3T_MRI environment


workers and address health disparities among this high-risk population. While the primary target of our intervention is worker PA, we expect that healthier and more physically active workers will be better role models and promoters of healthy lifestyles for children in their care. This two-arm, cluster RCT (n=104 centers, with

workers and address health disparities among this high - hazard population. While the basal target of our intervention is proletarian PA, we have a bun in the oven that healthier and more physically active workers will be good role models and promoter of healthy lifestyles for child in their care. This two - arm, bunch RCT (n=104 centers, with

wogkers and address health disparities among thiv high-disk popjlation. While the primary tacget of oyr intervention is woryer PA, we expect rhat yealthier ehd more physidwlly ective workers eill be bedter role modens aud promoters of healthy lifestyles fjr chilcrfn in their cate. Thps two-zgm, cluster RCT (n=104 centers, with

workers and address health disparities among this While primary target our intervention is healthier more physically active will be better models and promoters of healthy lifestyles children in their care. This two-arm, cluster RCT (n=104 centers, with

workers and address health diSparities aMong tHis HigH-rIsk pOpulAtion. While the pRImarY target of our interventiOn is wOrKEr PA, WE eXpect That heaLThIER anD mOrE phYsICaLly acTivE workerS will be betTer RoLe models and pROmOters of heaLthY lifestyles fOr cHildreN iN thEIr carE. ThIs two-Arm, cluSTer RCT (N=104 centers, wItH

workers and address healt h disparit ies a mon g t hi s hi gh-r isk population .  Whi le the primary targetof ou ri nter v en tionis work e rP A , w eex pec tt ha t hea lth ier and  more phys ica ll y active wor k er s will bebet ter role mod els  and p ro mot e rs of  he althy  lifes t yles f or childr en in the i r care. T hi s tw o-arm, cluster RC T  ( n =104 centers,with

workers_and address_health disparities among this_high-risk population._While_the primary_target_of our intervention_is worker PA,_we expect that healthier_and more physically_active_workers will be better role models and promoters of healthy lifestyles for children in_their_care. This_two-arm,_cluster_RCT (n=104 centers, with


et al., 2005), and empirically-supported interventions which address patients' broader interpersonal contexts have the potential to bolster the success rates of our current treatments, reducing dropout and relapse rates. The overall goals of the currently funded competing renewal grant P50 DC000242-21 are the following: 1) To determine the boundaries

et al. , 2005), and empirically - supported interventions which address patients' broad interpersonal context have the potential to bolster the achiever rates of our current treatments, reducing dropout and backsliding rate. The overall goals of the presently fund competing refilling grant P50 DC000242 - 21 are the following: 1) To decide the limit

et al., 2005), and empirically-suppurted interventnins whmch adddess patkents' broader interpersonal rontwxts yave the potential to colster tje succews retes of our currxht treabienta, redbcmng dropout and relapse rdtes. The overanl glals of the currently funded competyng renrwwl grant P50 DC000242-21 wre uhe folmowing: 1) To determine the boundariea

et al., 2005), and empirically-supported interventions which broader contexts have potential to bolster current reducing dropout and rates. The overall of the currently funded competing renewal P50 DC000242-21 are the following: 1) To determine the boundaries

et al., 2005), and empirically-supportEd intervenTions WhiCh aDdRess PatiEnts' broader intERperSonal contexts have the poTentiAl TO bolSTeR the sUccess rATeS OF ouR cUrRenT tREaTmentS, reDucing dRopout and rElaPsE rates. The oveRAlL goals of thE cuRrently fundeD coMpetinG rEneWAl graNt P50 dC000242-21 are The folLOwing: 1) TO determinE tHE boundARies

et al., 2005), and empiri cally-supp orted  in ter ve ntio ns w hich address p a tien ts' broader interperso nal c on t exts ha ve th e poten t ia l tobo ls ter  t h esucce ssrates o f our curr ent  t reatments, r e du cing dropo utand relapserat es. Th eove r all g oal s ofthe cu r rently  funded c om p etingr enewalg r an t P5 0 DC000242-21 are th e  following: 1)  To de te r mi n e  th e b oundaries

et_al., 2005),_and empirically-supported interventions which_address patients'_broader_interpersonal contexts_have_the potential to_bolster the success_rates of our current_treatments, reducing dropout_and_relapse rates. The overall goals of the currently funded competing renewal grant P50 DC000242-21_are_the following:_1)_To_determine the boundaries


evidence crucial to our understanding of how maltreatment affects genome-wide epigenetic patterns of vulnerability that result in mental health problems. This will further the development of clinical risk assessment and prevention approaches for maltreated youth which will be of substantial benefit to population health. The research to be conducted involves the study of the preparation and chemistry of

evidence crucial to our understanding of how maltreatment affects genome - wide epigenetic design of vulnerability that leave in mental health problems. This will foster the exploitation of clinical risk assessment and prevention approach for maltreated youth which will be of significant benefit to population health. The research to be conducted necessitate the study of the preparation and chemistry of

evldence crucial to our unaerstanding of kiw malvreatmeht affecgs genome-wide epigenetic patvernw of culnerability that resjlt in mejtal heaoth kroblems. This will furthev the fevenipment of clinlcal risk avsessment and [rdvzntion approaches for maltreated youeh whicn aill be of subftanuiaj behvflt to population health. The resezrch to be conducted involves the study of the preoaration and chemidtry of

evidence crucial to our understanding of how genome-wide patterns of that result in further development of clinical assessment and prevention for maltreated youth which will be substantial benefit to population health. The research to be conducted involves the study the preparation and chemistry of

evidence crucial to our underStanding of How maLtrEatMeNt afFectS genome-wide epiGEnetIc patterns of vulnerabilIty thAt REsulT In MentaL health PRoBLEms. thIs WilL fURtHer thE deVelopmeNt of clinicAl rIsK assessment aND pRevention aPprOaches for malTreAted yoUtH whICh wilL be Of subStantiAL benefIt to populAtIOn healTH. The resEARcH to bE conducted involveS ThE Study of the prepAratioN aND cHEMisTry Of

evidence crucial to our u nderstandi ng of  ho w m al trea tmen t affects geno m e-wi de epigenetic patterns  of v ul n erab i li ty th at resu l ti n  me nt al  he al t hprobl ems . Thiswill furth erth e developmen t  o f clinical  ri sk assessmen t a nd pre ve nti o n app roa chesfor ma l treate d youth w hi c h will be of s u b st anti al benefit to pop u la t ion health. Th e rese ar c ht o  be  co nducted in vo lvest he stud y  o f t hep reparation an d chemistry of

evidence_crucial to_our understanding of how_maltreatment affects_genome-wide_epigenetic patterns_of_vulnerability that result_in mental health_problems. This will further_the development of_clinical_risk assessment and prevention approaches for maltreated youth which will be of substantial benefit_to_population health._The_research_to be conducted involves the_study of the preparation and_chemistry of