the purified NADH oxidoreductase and cytochrome will be investigated with the enzymes reconstituted in phospholipid vesicular membranes containing lecithin with varying proportions of lysolecithin, cholesterol, and ethanolamine plasmalogen. A search for the function of the electron transfer system in the chromaffin granule

the purified NADH oxidoreductase and cytochrome will be investigated with the enzymes reconstituted in phospholipid vesicular membrane control lecithin with varying proportions of lysolecithin, cholesterol, and ethanolamine plasmalogen. A search for the function of the electron transportation system in the chromaffin granule

thf purified NADH oxidoredmctase and cytockeome wmll be jnvestigxted with the enzymes reconsvitured ib phospholipid vesiculxr membrajes contqiniig lecithin with varying propodbions if lysolecithik, cholesterml, and ethanoldmknz plasmalogen. A search for the functyon of yhf electron tragsfeg fystsm in the chromaffin granule

the purified NADH oxidoreductase and cytochrome will with enzymes reconstituted phospholipid vesicular membranes of cholesterol, and ethanolamine A search for function of the electron transfer system the chromaffin granule

the purified NADH oxidoreducTase and cytOchroMe wIll Be InveStigAted with the enzYMes rEconstituted in phospholIpid vEsICulaR MeMbranEs contaINiNG LecItHiN wiTh VArYing pRopOrtions Of lysoleciThiN, cHolesterol, anD EtHanolamine PlaSmalogen. A seaRch For the FuNctIOn of tHe eLectrOn tranSFer sysTem in the cHrOMaffin GRanule

the purified NADH oxidore ductase an d cyt och rom ewill  beinvestigated w i th t he enzymes reconstitut ed in  p h osph o li pid v esicula r  m e m bra ne scon ta i ni ng le cit hin wit h varyingpro po rtions of ly s ol ecithin, c hol esterol, and  et hanola mi nep lasma log en. A  searc h  for t he functi on of the electro n tr ansf er system in thec hr o maffin granule

the_purified NADH_oxidoreductase and cytochrome will_be investigated_with_the enzymes_reconstituted_in phospholipid vesicular_membranes containing lecithin_with varying proportions of_lysolecithin, cholesterol, and_ethanolamine_plasmalogen. A search for the function of the electron transfer system in the chromaffin_granule


Angela Koehler. These Programs function to stimulate new research initiatives by their Members as well as to foster intra- and inter-programmatic collaborations. In aggregate the 56 Members of this Center have published 1006 cancer-related articles over the past grant period. Of those, nearly 18% have involved multiple Members.

Angela Koehler. These Programs function to stimulate new research enterprise by their extremity as well as to foster intra- and inter - programmatic collaborations. In aggregate the 56 member of this Center have published 1006 cancer - related article over the past concession period. Of those, nearly 18% have involved multiple Members.

Anhela Koehler. These Progrxms function to stimulete new researcf initiatives by their Membecs aw welo as to foster intra- avd inter-pgogrammatuc ciolaboratioia. In agnxegats the 56 Members of thls Center hdve published 1006 cxneer-related articles over the past grwnt peroof. Of those, neatly 18% nwve jnvolved multiple Members.

Angela Koehler. These Programs function to stimulate initiatives their Members well as to In the 56 Members this Center have 1006 cancer-related articles over the past period. Of those, nearly 18% have involved multiple Members.

Angela Koehler. These ProgramS function tO stimUlaTe nEw ReseArch Initiatives by tHEir MEmbers as well as to foster Intra- AnD InteR-PrOgramMatic coLLaBORatIoNs. in aGgREgAte thE 56 MeMbers of This Center HavE pUblished 1006 cancER-rElated artiCleS over the past GraNt periOd. of tHOse, neArlY 18% have InvolvED multiPle MemberS.

Angela Koehler. These Pro grams func tiontosti mu late  new  research init i ativ es by their Members as  well  a s  tof os ter i ntra- a n di n ter -p ro gra mm a ti c col lab oration s. In aggr ega te  the 56 Memb e rs  of this C ent er have publ ish ed 100 6can c er-re lat ed ar ticles over t he past g ra n t peri o d. Of t h o se , ne arly 18% have inv o lv e d multiple Mem bers.

Angela_Koehler. These_Programs function to stimulate_new research_initiatives_by their_Members_as well as_to foster intra-_and inter-programmatic collaborations. In_aggregate the 56_Members_of this Center have published 1006 cancer-related articles over the past grant period. Of_those,_nearly 18%_have_involved_multiple Members.


lebotomy with hepatic iron concentration. The primary efficacy endpoints are the response rate (based on loss of hepatitis C RNA in serum) at the end of a 6-12 month course of interferon, and the ability to sustain that response for 6 months after the end of therapy. This application seeks support for Pediatric Oncology

lebotomy with hepatic iron concentration. The primary efficacy endpoints are the response pace (free-base on loss of hepatitis C RNA in serum) at the goal of a 6 - 12 calendar month course of interferon, and the ability to sustain that response for 6 calendar month after the end of therapy. This application seek support for Pediatric Oncology

lebltomy with hepatic iron goncentration. Thg primarb efficzcy endpuints are the response rate (uasee on ooss of hepatitis C RNX in serul) at the end if a 6-12 monti course of infcrfermi, and the abilijy to sustait that responsa wox 6 months after the end of therapy. Tris appkifation seeks sopporu fjr Psdiatric Oncology

lebotomy with hepatic iron concentration. The primary are response rate on loss of at end of a month course of and the ability to sustain that for 6 months after the end of therapy. This application seeks support for Oncology

lebotomy with hepatic iron coNcentratioN. The pRimAry EfFicaCy enDpoints are the rESponSe rate (based on loss of hepAtitiS C rnA in SErUm) at tHe end of A 6-12 MoNTH coUrSe Of iNtERfEron, aNd tHe abiliTy to sustaiN thAt Response for 6 mONtHs after the End Of therapy. ThiS apPlicatIoN seEKs supPorT for PEdiatrIC OncolOgy

lebotomy with hepatic iron  concentra tion.  Th e p ri mary  eff icacy endpoint s  are  the response rate (ba sed o nl osso fhepat itis CR NA i n s er um ) a tt he  endofa 6-12month cour seof  interferon, an d the abil ity  to sustaintha t resp on sef or 6mon ths a fter t h e endof therap y. This a p plicati o n  s eeks  support for Pedi a tr i c Oncology

lebotomy with_hepatic iron_concentration. The primary efficacy_endpoints are_the_response rate_(based_on loss of_hepatitis C RNA_in serum) at the_end of a_6-12_month course of interferon, and the ability to sustain that response for 6 months_after_the end_of_therapy._This application seeks support for_Pediatric Oncology


-1 genes. We have made SHIV clones containing the envelope (env) gene of various HIV-1 subtype-B isolates, and have analyzed these recombinant viruses in vivo in juvenile and newborn macaques. Results A pathogenic SHIV (designated SHIV-33A), containing the env gene of HIV-

-1 genes. We have made SHIV clones containing the envelope (env) gene of diverse HIV-1 subtype - boron isolates, and have analyzed these recombinant viruses in vivo in juvenile and newborn macaque. Results A pathogenic SHIV (designated SHIV-33A), contain the env gene of HIV-

-1 gejes. We have made SHIV clunes containing the entelope (snv) gene of various HIV-1 subtype-B isopares, abd have analyzed these recombinwnt viruwes mn vivo in juvenmme and kzwborh maccqnes. Results A psthogenic VHIV (designateg RHNV-33A), containing the env gene of HIV-

-1 genes. We have made SHIV clones envelope gene of HIV-1 subtype-B isolates, viruses vivo in juvenile newborn macaques. Results pathogenic SHIV (designated SHIV-33A), containing the gene of HIV-

-1 genes. We have made SHIV clones Containing The enVelOpe (EnV) genE of vArious HIV-1 subtyPE-B isOlates, and have analyzed tHese rEcOMbinANt VirusEs in vivO In JUVenIlE aNd nEwBOrN macaQueS. ResultS A pathogenIc ShIv (designated ShiV-33a), containinG thE env gene of HIv-

-1 genes. We have made SHI V clones c ontai nin g t he  env elop e (env) gene o f  var ious HIV-1 subtype-B i solat es ,  and ha ve an alyzedt he s e  re co mb ina nt vi ruses  in  vivo i n juvenile  an dnewborn maca q ue s. Results  Apathogenic S HIV  (desi gn ate d  SHIV -33 A), c ontain i ng the  env gene  o f  HIV-

-1 genes._We have_made SHIV clones containing_the envelope_(env)_gene of_various_HIV-1 subtype-B isolates,_and have analyzed_these recombinant viruses in_vivo in juvenile_and_newborn macaques. Results A pathogenic SHIV (designated SHIV-33A), containing the env gene of HIV-


their relationships with their spouses, children, families, and friends, b) the women's responsiveness to their infants, and c) the infants' affective expression and emotional regulation; (3) to determine the rate of recurrence of depression in successfully treated women over an 18 month period following the end of treatment; and (4

their relationships with their spouses, children, families, and acquaintance, b) the charwoman's responsiveness to their infants, and c) the infants' affective formulation and emotional regulation; (3) to specify the rate of recurrence of depression in successfully treat women over an 18 month menstruation following the end of discussion; and (4

thfir relationships with tmeir spouses, children, femilies, and fridnds, b) the women's responsiveiess to tyeir infants, and c) the infants' wffectivw exkression and emotmknal renblatikk; (3) to vetermine the rste of recgrrence of depsersnon in successfully treated women ovqr an 18 kojth period foljowimd ths end of treatment; and (4

their relationships with their spouses, children, families, b) women's responsiveness their infants, and and regulation; (3) to the rate of of depression in successfully treated women an 18 month period following the end of treatment; and (4

their relationships with theIr spouses, cHildrEn, fAmiLiEs, anD friEnds, b) the women's REspoNsiveness to their infantS, and c) ThE InfaNTs' AffecTive expREsSIOn aNd EmOtiOnAL rEgulaTioN; (3) to deteRmine the raTe oF rEcurrence of dEPrEssion in suCceSsfully treatEd wOmen ovEr An 18 mONth peRioD follOwing tHE end of Treatment; AnD (4

their relationships withtheir spou ses,chi ldr en , fa mili es, and friend s , b)  the women's responsiv eness  t o  the i rinfan ts, and c) t hein fa nts 'a ff ectiv e e xpressi on and emo tio na l regulation ;  ( 3) to dete rmi ne the rateofrecurr en ceo f dep res sionin suc c essful ly treate dw omen o v er an 1 8 mo nthperiod followingt he end of treatme nt; an d( 4

their_relationships with_their spouses, children, families,_and friends,_b)_the women's_responsiveness_to their infants,_and c) the_infants' affective expression and_emotional regulation; (3)_to_determine the rate of recurrence of depression in successfully treated women over an 18_month_period following_the_end_of treatment; and (4


Bioinformatics Core will be available to provide the requisite expertise for data analysis, including more conventional statistical analysis of all types of data and analysis of data generated by microarray or proteomics techniques. Inflammation or peripheral nerve injury increases neuropeptide Y (NPY) expression in pain-relevant areas of the nervous system, such

Bioinformatics Core will be available to provide the requisite expertise for data psychoanalysis, include more conventional statistical analysis of all type of datum and analysis of data generated by microarray or proteomics technique. Inflammation or peripheral nerve wound increase neuropeptide Y (NPY) expression in annoyance - relevant areas of the nervous system, such

Bilinformatics Core will bt available to provide tie requjsite exoertise for data analysis, inrludung mire conventional statirtical anwlysis od alo types of vzta and analyals of vata generated ny microarrdy or proteomiws tzchniques. Inflammation or peripheral nerve onuury increases neugo[eptjde Y (NPY) expression in pain-relevaht areav of the nervpus system, such

Bioinformatics Core will be available to provide expertise data analysis, more conventional statistical data analysis of data by microarray or techniques. Inflammation or peripheral nerve injury neuropeptide Y (NPY) expression in pain-relevant areas of the nervous system, such

Bioinformatics Core will be aVailable to ProviDe tHe rEqUisiTe exPertise for data ANalySis, including more convenTionaL sTAtisTIcAl anaLysis of ALl TYPes Of DaTa aNd ANaLysis Of dAta geneRated by micRoaRrAy or proteomiCS tEchniques. INflAmmation or peRipHeral nErVe iNJury iNcrEases NeuropEPtide Y (nPY) expresSiON in paiN-RelevanT AReAs of The nervous system, sUCh

Bioinformatics Core willbe availab le to  pr ovi de  the  req uisite experti s e fo r data analysis, inclu dingmo r e co n ve ntion al stat i st i c alan al ysi so fall t ype s of da ta and ana lys is  of data gen e ra ted by mic roa rray or prot eom ics te ch niq u es. I nfl ammat ion or periph eral nerv ei njuryi ncrease s ne urop eptide Y (NPY) ex p re s sion in pain-r elevan ta re a s  of  th e nervoussy stem, such

Bioinformatics_Core will_be available to provide_the requisite_expertise_for data_analysis,_including more conventional_statistical analysis of_all types of data_and analysis of_data_generated by microarray or proteomics techniques. Inflammation or peripheral nerve injury increases neuropeptide Y_(NPY)_expression in_pain-relevant_areas_of the nervous system, such


, likely play an important role in longer trm outcome. In this competitive renewal, we will extend follow-up of our cohort to 6 years of age and focus on structure/function relationships and the predictive value of early brain structure for later childhood brain structure and cognitive function. MRIs, including structural and diffusion tensor imaging

, likely play an important role in long trm outcome. In this competitive reclamation, we will extend follow - up of our cohort to 6 long time of age and focus on social organization / function relationships and the predictive value of early brain social organization for belated childhood brain social organization and cognitive function. MRIs, including structural and dissemination tensor imaging

, linely play an important rule in longer ttm outcoke. In fhis comoetitive renewal, we will extxnd dolloq-up of our cohort to 6 hears of wge and docuw on structnde/functljn rspatimiships and the kredictive vdlue of early trxiu structure for later childhood braig strucyuge and cognitide flnstioh. MRIs, including structural and dirfusion tensor imagimg

, likely play an important role in outcome. this competitive we will extend 6 of age and on structure/function relationships the predictive value of early brain for later childhood brain structure and cognitive function. MRIs, including structural and diffusion imaging

, likely play an important role In longer trM outcOme. in tHiS comPetiTive renewal, we wILl exTend follow-up of our cohorT to 6 yeArS Of agE AnD focuS on struCTuRE/FunCtIoN reLaTIoNshipS anD the preDictive valUe oF eArly brain strUCtUre for lateR chIldhood brain StrUcture AnD coGNitivE fuNctioN. MRIs, iNCludinG structurAl ANd diffUSion tenSOR iMagiNg

, likely play an important  role in l onger  tr m o ut come . In  this competit i ve r enewal, we will extend  foll ow - up o f  o ur co hort to 6y e ars  o fage  a n dfocus  on  struct ure/functi onre lationshipsa nd  the predi cti ve value ofear ly bra in  st r uctur e f or la ter ch i ldhood  brain st ru c ture a n d cogni t i ve  fun ction. MRIs, incl u di n g structural a nd dif fu s io n ten sor  imaging

, likely_play an_important role in longer_trm outcome._In_this competitive_renewal,_we will extend_follow-up of our_cohort to 6 years_of age and_focus_on structure/function relationships and the predictive value of early brain structure for later childhood_brain_structure and_cognitive_function._MRIs, including structural and diffusion_tensor imaging


in clinical use. An attempt is now being made to develop a specific assay for the hybrid MB species. Approximately 10-20% of adults experience chronic abdominal symptoms (abdominal pain/discomfort and associated bowel changes [constipation and/or diarrhea] compatible with a diagnosis of irritable bowel syndrome (IBS).

in clinical use. An attempt is now being made to originate a specific assay for the hybrid megabyte species. Approximately 10 - 20% of adults experience chronic abdominal symptom (abdominal pain / discomfort and associated intestine changes [ constipation and/or diarrhea ] compatible with a diagnosis of irritable intestine syndrome (IBS).

in clinical use. An attempt is now being mcee to vevelop a speciwic assay for the hybrid MB dpwcies. Approximately 10-20% of aduuts experpence chrinic qbdominal symptoms (abdomjkal pcii/discomfort and associateg bowel changev [zoustipation and/or diarrhea] compatible with a dlagnosis of irtitabkq bosvl syndrome (IBS).

in clinical use. An attempt is now to a specific for the hybrid adults chronic abdominal symptoms pain/discomfort and associated changes [constipation and/or diarrhea] compatible with diagnosis of irritable bowel syndrome (IBS).

in clinical use. An attempt is nOw being madE to deVelOp a SpEcifIc asSay for the hybriD mB spEcies. Approximately 10-20% of adUlts eXpERienCE cHroniC abdomiNAl SYMptOmS (aBdoMiNAl Pain/dIscOmfort aNd associatEd bOwEl changes [conSTiPation and/oR diArrhea] compatIblE with a DiAgnOSis of IrrItablE bowel SYndromE (IBS).

in clinical use. An attem pt is nowbeing  ma deto  dev elop  a specific as s ay f or the hybrid MB speci es. A pp r oxim a te ly 10 -20% of ad u l tsex pe rie nc e  c hroni c a bdomina l symptoms  (a bd ominal pain/ d is comfort an d a ssociated bo wel  chang es  [c o nstip ati on an d/or d i arrhea ] compati bl e  witha  diagno s i sof i rritable bowel sy n dr o me (IBS).

in_clinical use._An attempt is now_being made_to_develop a_specific_assay for the_hybrid MB species._Approximately 10-20% of adults_experience chronic abdominal_symptoms_(abdominal pain/discomfort and associated bowel changes [constipation and/or diarrhea] compatible with a diagnosis of_irritable_bowel syndrome_(IBS).


for cytotoxicity and intra-cellular delivery of DTG. The ability of DTG-gel and CAP-NPs-DTG-Gel to offer protection from HIV-1 infection will be tested in TZM-bl cells, PBMCs, and MDMs. We will evaluate vaginal pharmacokinetics of D

for cytotoxicity and intra - cellular delivery of DTG. The ability of DTG - gel and CAP - NPs - DTG - Gel to offer protection from HIV-1 infection will be quiz in TZM - bl cell, PBMCs, and MDMs. We will evaluate vaginal pharmacokinetics of D

fog cytotoxicity and intra-gellular deliveri if DTG. The agility ow DTG-gel and CAP-NPs-DTG-Gel to odfer kgotection from HIV-1 inwection wpll be tewted un TZM-bl cxmls, PBMGf, ans MDMv. We will evaluste vaginan pharmacokinedizs of D

for cytotoxicity and intra-cellular delivery of DTG. of and CAP-NPs-DTG-Gel offer protection from in cells, PBMCs, and We will evaluate pharmacokinetics of D

for cytotoxicity and intra-ceLlular deliVery oF DTg. ThE aBiliTy of dTG-gel and CAP-NPS-dTG-GEl to offer protection froM HIV-1 iNfECtioN WiLl be tEsted in tzM-BL CelLs, pBmCs, AnD mDms. We wIll EvaluatE vaginal phArmAcOkinetics of D

for cytotoxicity and intr a-cellular  deli ver y o fDTG.  The  ability of DT G -gel  and CAP-NPs-DTG-Gel t o off er prot e ct ion f rom HIV - 1i n fec ti on  wi ll be  test edin TZM- bl cells,PBM Cs , and MDMs.W ewill evalu ate  vaginal pha rma cokine ti cso f D

for_cytotoxicity and_intra-cellular delivery of DTG._The ability_of_DTG-gel and_CAP-NPs-DTG-Gel_to offer protection_from HIV-1 infection_will be tested in_TZM-bl cells, PBMCs,_and_MDMs. We will evaluate vaginal pharmacokinetics of D


order to develop these approaches, a greater understanding of factors that control viral latency is needed. Efforts in this regard have been hampered by the lack of a suitable in vitro primary T cell model of latency. The studies proposed herein will utilize a new in vitro model to characterize multiple aspects of HIV latency including defining viral and host

order to develop these approaches, a greater reason of divisor that control viral latency is needed. effort in this regard have been hampered by the lack of a desirable in vitro primary T cell exemplar of latency. The studies nominate herein will utilize a new in vitro mannequin to qualify multiple aspects of HIV latency include defining viral and host

orfer to develop these appvoaches, a greatet yndersvanding of facturs that control viral latenry iw neeeed. Efforts in this reeard have been hanpertd by the lack of a suitanje ih vitxo primary T celk model of latency. The sduaizs proposed herein will utilize a ner in viyrl model to chatactegise mhltiple aspects of HIV latency incmuding vefining viral snd host

order to develop these approaches, a greater factors control viral is needed. Efforts hampered the lack of suitable in vitro T cell model of latency. The proposed herein will utilize a new in vitro model to characterize multiple aspects HIV latency including defining viral and host

order to develop these approaChes, a greatEr undErsTanDiNg of FactOrs that control VIral Latency is needed. Efforts In thiS rEGard HAvE been HampereD By THE laCk Of A suItABlE in viTro Primary t cell model Of lAtEncy. The studiES pRoposed herEin Will utilize a New In vitrO mOdeL To chaRacTerizE multiPLe aspeCts of HIV lAtENcy incLUding deFINiNg viRal and host

order to develop these ap proaches,a gre ate r u nd erst andi ng of factorst hatcontrol viral latencyis ne ed e d. E f fo rts i n thisr eg a r d h av ebee nh am pered  by  the la ck of a su ita bl e in vitro p r im ary T cell  mo del of laten cy.  The s tu die s  prop ose d her ein wi l l util ize a new  i n  vitro model t o ch arac terize multiple a s pe c ts of HIV late ncy in cl u di n g  de fin ing viralan d hos t

order_to develop_these approaches, a greater_understanding of_factors_that control_viral_latency is needed._Efforts in this_regard have been hampered_by the lack_of_a suitable in vitro primary T cell model of latency. The studies proposed herein_will_utilize a_new_in_vitro model to characterize multiple_aspects of HIV latency including_defining viral_and host


Aim 1, we will characterize the role of HVEM and nectin-1 in primary HSV-1 infection of the cornea by determining whether HVEM and/or nectin-1 are required for primary HSV-1 infection of the cornea. We will also evaluate the importance of input viral load on the development

Aim 1, we will characterize the role of HVEM and nectin-1 in primary HSV-1 infection of the cornea by determine whether HVEM and/or nectin-1 are command for primary HSV-1 infection of the cornea. We will also measure the importance of input viral load on the development

Ail 1, we will characterize uhe role of HVEM cbd necvin-1 in lrimary FSV-1 infection of the cornea uy dwtermuning whether HVEM and/ur nectin-1 are reqyirev for primary HST-1 infectljn or the rornea. We will slso evaludte the importdnze of input viral load on the developient

Aim 1, we will characterize the role and in primary infection of the and/or are required for HSV-1 infection of cornea. We will also evaluate the of input viral load on the development

Aim 1, we will characterize the rOle of HVEM aNd necTin-1 In pRiMary hSV-1 iNfection of the cORnea By determining whether HVeM and/Or NEctiN-1 ArE requIred for PRiMARy HsV-1 InFecTiON oF the cOrnEa. We wilL also evaluAte ThE importance oF InPut viral loAd oN the developmEnt

Aim 1, we will characteri ze the rol e ofHVE M a nd  nec tin- 1 in primary H S V-1infection of the corne a byde t ermi n in g whe ther HV E Ma n d/o rne cti n- 1  a re re qui red for  primary H SV- 1infection of th e cornea.Wewill also ev alu ate th eimp o rtanc e o f inp ut vir a l load  on the d ev e lopmen t

Aim_1, we_will characterize the role_of HVEM_and_nectin-1 in_primary_HSV-1 infection of_the cornea by_determining whether HVEM and/or_nectin-1 are required_for_primary HSV-1 infection of the cornea. We will also evaluate the importance of input_viral_load on_the_development


Pyk2/GPR30/Vav1 complex. Aim 2: To determine the mechanism by which estrogen-induced Pyk2 protein- protein interactions signal Id3 to block p21Cip1 expression. The long-term goal of this research project is to determine whether in vivo estrogen-induced Pyk

Pyk2 / GPR30 / Vav1 complex. Aim 2: To determine the mechanism by which estrogen - induced Pyk2 protein- protein interaction sign Id3 to block p21Cip1 expression. The long - terminus goal of this research undertaking is to determine whether in vivo estrogen - induced Pyk

Pyn2/GPR30/Vav1 complex. Aim 2: To aetermine the mgcyanism by whjch estrugen-induced Pyk2 protein- protxin unterqctions signal Id3 to buock p21Cip1 expressuon. Uhe long-term goal of this reseadgh prmoect is to detetmine whethes in vivo estrmgdn-nnduced Pyk

Pyk2/GPR30/Vav1 complex. Aim 2: To determine the which Pyk2 protein- interactions signal Id3 long-term of this research is to determine in vivo estrogen-induced Pyk

Pyk2/GPR30/Vav1 complex. Aim 2: To deteRmine the meChaniSm bY whIcH estRogeN-induced Pyk2 proTEin- pRotein interactions signAl Id3 tO bLOck p21cIp1 ExpreSsion. ThE LoNG-TerM gOaL of ThIS rEsearCh pRoject iS to determiNe wHeTher in vivo esTRoGen-induced pyk

Pyk2/GPR30/Vav1 complex.Aim 2: Todeter min e t he  mec hani sm by which es t roge n-induced Pyk2 protein - pro te i n in t er actio ns sign a lI d 3 t obl ock  p 2 1C ip1 e xpr ession.  The long- ter mgoal of this re search pro jec t is to dete rmi ne whe th eri n viv o e strog en-ind u ced Py k

Pyk2/GPR30/Vav1_complex. Aim_2: To determine the_mechanism by_which_estrogen-induced Pyk2_protein-_protein interactions signal_Id3 to block_p21Cip1 expression. The long-term_goal of this_research_project is to determine whether in vivo estrogen-induced Pyk


our training and graduate programs, including a summer program bringing faculty and undergraduate students from minority serving institutions to NC State during the summer to initiate collaborative work with training faculty. We have developed databases and software useful for comparative analysis of protein three-dimensional structure. These tools are distributed freely to biologists and developers of biotechnology software

our training and graduate programs, including a summer platform bring faculty and undergraduate students from minority serving institution to NC State during the summer to initiate collaborative work with education faculty. We have developed databases and software utilitarian for relative analysis of protein three - dimensional structure. These cock are distributed freely to biologists and developers of biotechnology software

oug training and graduate krograms, includiny a sumker prkgram brknging faculty and undergradnate studtuts from minority sefving insnitutions to IC State during vge summcx to jkitiace collaborative work with training facunth. Ce have developed databases and softrare usrfkl for comparajive sgalyaps of protein three-dimensional atructuge. These tools arr distributed freely to billoglsts and developerd of biotecynoljty software

our training and graduate programs, including a bringing and undergraduate from minority serving the to initiate collaborative with training faculty. have developed databases and software useful comparative analysis of protein three-dimensional structure. These tools are distributed freely to biologists developers of biotechnology software

our training and graduate proGrams, incluDing a SumMer PrOgraM briNging faculty anD UndeRgraduate students from mInoriTy SErviNG iNstitUtions tO nC sTAte DuRiNg tHe SUmMer to IniTiate coLlaborativE woRk With training FAcUlty. We have DevEloped databaSes And sofTwAre USeful For CompaRative ANalysiS of proteiN tHRee-dimENsional STRuCturE. These tools are disTRiBUted freely to biOlogisTs ANd DEVelOpeRs of biotecHnOlogy SOftware

our training and graduate  programs,  incl udi ngasumm er p rogram bringin g  fac ulty and undergraduate  stud en t s fr o mminor ity ser v in g ins ti tu tio ns to  NC S tat e durin g the summ erto  initiate co l la borative w ork  with traini ngfacult y.  We havedev elope d data b ases a nd softwa re useful for com p a ra tive  analysis of prot e in three-dimensio nal st ru c tu r e . T hes e tools ar edistr i buted f r ee l y tob iologists and  developers ofbiotec hn olo g y soft ware

our_training and_graduate programs, including a_summer program_bringing_faculty and_undergraduate_students from minority_serving institutions to_NC State during the_summer to initiate_collaborative_work with training faculty. We have developed databases and software useful for comparative analysis_of_protein three-dimensional_structure._These_tools are distributed freely to_biologists and developers of biotechnology_software


the terminal vasculature. The objective of this grant proposal is to map the frontal eye fields (FEF) in monkeys by microstimulation and to determine the neuronal connections of this cortical area by light microscopy. The FEF will be defined as the area which yields low threshold (20 - 50uamp) eye movement

the terminal vasculature. The objective of this grant proposal is to map the frontal center field (FEF) in monkeys by microstimulation and to determine the neural connections of this cortical sphere by light microscopy. The FEF will be defined as the sphere which yields low threshold (20 - 50uamp) center campaign

thf terminal vasculature. Tme objective of jhus grait propksal is go map the frontal eye fieldd (DEF) ib monkeys by microstimjlation ajd to derermmne the neuronal connectljns kn thiv cortical area by light kicroscopy. The FDF will be defined as the area which rields koa threshold (20 - 50oamp) tye movsment

the terminal vasculature. The objective of this is map the eye fields (FEF) to the neuronal connections this cortical area light microscopy. The FEF will be as the area which yields low threshold (20 - 50uamp) eye movement

the terminal vasculature. The Objective oF this GraNt pRoPosaL is tO map the frontal EYe fiElds (FEF) in monkeys by micrOstimUlATion ANd To detErmine tHE nEURonAl CoNneCtIOnS of thIs cOrtical Area by lighT miCrOscopy. The FEF WIlL be defined As tHe area which yIelDs low tHrEshOLd (20 - 50uamP) eyE moveMent

the terminal vasculature.  The objec tiveofthi sgran t pr oposal is to m a p th e frontal eye fields ( FEF)in monk e ys  by m icrosti m ul a t ion  a nd  to  d e te rmine  th e neuro nal connec tio ns  of this cor t ic al area by  li ght microsco py.  The F EF  wi l l bedef inedas the area w hich yiel ds low th r eshold( 2 0- 50 uamp) eye movemen t

the_terminal vasculature._The objective of this_grant proposal_is_to map_the_frontal eye fields_(FEF) in monkeys_by microstimulation and to_determine the neuronal_connections_of this cortical area by light microscopy. The FEF will be defined as the_area_which yields_low_threshold_(20 - 50uamp) eye movement


in changing children's knowledge, attitudes and intended behaviors toward including a greater variety of fruits/vegetables and low-fat foods in their diet, Aim 5) secure and implement distribution commitments from Philips for Phase III commercialization of the final CD-I. After interacting with Pyramid Adventures, children will select

in changing children's knowledge, attitudes and mean demeanor toward including a greater variety of yield / vegetables and low - fatty foods in their diet, Aim 5) secure and implement distribution committedness from Philips for Phase III commercialization of the final CD - I. After interacting with Pyramid Adventures, child will select

in changing children's knowuedge, attitudes and invended gehaviorr toward including a greater vqriett of fruits/vegetables xnd low-fan foods ib thtir diet, Aim 5) secnde and liplejcnt dnsvribution commijments from [hilips for Phdsd NII commercialization of the final CQ-I. Aftet lnteracting wijh Pygaiid Zdventures, children will select

in changing children's knowledge, attitudes and intended including greater variety fruits/vegetables and low-fat 5) and implement distribution from Philips for III commercialization of the final CD-I. interacting with Pyramid Adventures, children will select

in changing children's knowleDge, attitudEs and IntEndEd BehaViorS toward includiNG a grEater variety of fruits/veGetabLeS And lOW-fAt fooDs in theIR dIET, AiM 5) sEcUre AnD ImPlemeNt dIstribuTion commitMenTs From Philips fOR PHase III comMerCialization oF thE final cD-i. AfTEr intEraCting With PyRAmid AdVentures, cHiLDren wiLL select

in changing children's kn owledge, a ttitu des  an dinte nded  behaviors tow a rd i ncluding a greater var ietyof frui t s/ veget ables a n dl o w-f at  f ood si ntheir  di et, Aim  5) secure  an dimplement di s tr ibution co mmi tments fromPhi lips f or  Ph a se II I c ommer cializ a tion o f the fin al CD-I.A fter in t e ra ctin g with Pyramid Ad v en t ures, children  willse l ec t

in_changing children's_knowledge, attitudes and intended_behaviors toward_including_a greater_variety_of fruits/vegetables and_low-fat foods in_their diet, Aim 5)_secure and implement_distribution_commitments from Philips for Phase III commercialization of the final CD-I. After interacting with_Pyramid_Adventures, children_will_select


the S100 family of calcium binding proteins, the members of which may play a role in mediating the effects of calcium in a variety of other tissues. Because of its tissue-specific expression in the olfactory epithelium, the regulation of the ictacalcin gene is potentially interesting. In this application, Dr. Porta

the S100 family of calcium binding proteins, the members of which may toy a function in mediating the effects of calcium in a variety of early tissues. Because of its tissue - specific expression in the olfactory epithelium, the regulation of the ictacalcin gene is potentially interesting. In this lotion, Dr. Porta

thf S100 family of calcium bikding proteins, tkw membxrs of shich mah play a role in mediating tie edfectw of calcium in a varidty of otjer tissyes. Uecause of its tmasue-spegnfic swpresvmon in the olfagtory epithalium, the regunagiln of the ictacalcin gene is potentyally imtfresting. In thys akplycatjon, Dr. Porta

the S100 family of calcium binding proteins, of may play role in mediating a of other tissues. of its tissue-specific in the olfactory epithelium, the regulation the ictacalcin gene is potentially interesting. In this application, Dr. Porta

the S100 family of calcium bindinG proteins, tHe memBerS of WhIch mAy plAy a role in mediaTIng tHe effects of calcium in a vArietY oF OtheR TiSsues. because OF iTS TisSuE-sPecIfIC eXpresSioN in the oLfactory epIthElIum, the regulaTIoN of the ictaCalCin gene is potEntIally iNtEreSTing. IN thIs appLicatiON, Dr. PorTa

the S100 family of calciu m bindingprote ins , t he  mem bers  of which mayp laya role in mediating th e eff ec t s of ca lcium  in a v a ri e t y o fot her  t i ss ues.Bec ause of  its tissu e-s pe cific expres s io n in the o lfa ctory epithe liu m, the  r egu l ation  of  theictaca l cin ge ne is pot en t iallyi nterest i n g.  Inthis application, Dr .  Porta

the_S100 family_of calcium binding proteins,_the members_of_which may_play_a role in_mediating the effects_of calcium in a_variety of other_tissues._Because of its tissue-specific expression in the olfactory epithelium, the regulation of the ictacalcin_gene_is potentially_interesting._In_this application, Dr. Porta


, I varied visual attention, which improves perception of an attended location or feature. The amount of information encoded in a population of neurons is limited by both the variability of individual cortical neurons and by correlated variability that is shared across the population (noise correlations). I found that attentional changes in shared variability are likely the major contributor

, I varied visual attention, which improves percept of an attend location or feature. The amount of data encoded in a population of neurons is limited by both the unevenness of individual cortical nerve cell and by correlated variability that is shared across the population (noise correlations). I find that attentional changes in partake variability are likely the major contributor

, I garied visual attention, dhich improves keeceptimn of zn attenaed location or feature. The emoubt of information encoded iv a populwtion of neucons is limited ug both bke vadlabilntb of individual cortical teurons and by curxelated variability that is shared asross tne population (noyse bowrelzniins). I found that attentional dhanges in shared vatiability are likely the mwjor contributor

, I varied visual attention, which improves an location or The amount of of is limited by the variability of cortical neurons and by correlated variability is shared across the population (noise correlations). I found that attentional changes in variability are likely the major contributor

, I varied visual attention, whiCh improves PercePtiOn oF aN attEndeD location or feaTUre. THe amount of information eNcodeD iN A popULaTion oF neuronS Is LIMitEd By BotH tHE vAriabIliTy of indIvidual corTicAl Neurons and by COrRelated varIabIlity that is sHarEd acroSs The POpulaTioN (noisE correLAtions). i found thaT aTTentioNAl changES In SharEd variability are lIKeLY the major contrIbutor

, I varied visual attentio n, which i mprov esper ce ptio n of  an attended l o cati on or feature. The amo unt o fi nfor m at ion e ncodedi na pop ul at ion  o f  n euron s i s limit ed by both  th evariabilityo findividual  co rtical neuro nsand by  c orr e lated  va riabi lity t h at isshared ac ro s s thep opulati o n  ( nois e correlations).I  f o und that atten tional  c h an g e s i n s hared vari ab ility are lik e ly t h e m a jor contribut or

, I_varied visual_attention, which improves perception_of an_attended_location or_feature._The amount of_information encoded in_a population of neurons_is limited by_both_the variability of individual cortical neurons and by correlated variability that is shared across_the_population (noise_correlations)._I_found that attentional changes in_shared variability are likely the_major contributor


Further research and development of these Fc mutants should lead to therapeutic antibodies with increased capacity to trigger antibody dependent cytotoxic reactions against tumor cells. This proposal describes an experimental paradigm to identify Fc mutations, characterize the mutations in functional assays, and ultimately test therapeutic antibodies harboring the best Fc mutations in animal model studies. DESCRIPTION: Abstract Tra

Further research and development of these Fc mutants should lead to curative antibody with increase capacity to trip antibody dependent cytotoxic reaction against tumor cells. This marriage proposal describes an experimental paradigm to identify Fc mutant, characterize the mutations in running assays, and ultimately test therapeutic antibodies harbor the best Fc mutations in animal model studies. DESCRIPTION: Abstract Tra

Fugther research and develupment of these Fc mutents shkuld leaa to therapeutic antibodies xith incrtcsed capacity to trieger antihody depwndeit cytotoxic rearfions ancinst bumor rells. This proppsal descrhbes an experikevtcl paradigm to identify Fc mutations, characyegize the mutatyons yn fhnctional assays, and ultimately teat therepeutic antibodoes harboring the best Fc lutahions in animal mofel studies. DESSEIPTION: Abstfact Tra

Further research and development of these Fc lead therapeutic antibodies increased capacity to against cells. This proposal an experimental paradigm identify Fc mutations, characterize the mutations functional assays, and ultimately test therapeutic antibodies harboring the best Fc mutations in model studies. DESCRIPTION: Abstract Tra

Further research and developMent of thesE Fc muTanTs sHoUld lEad tO therapeutic anTIbodIes with increased capaciTy to tRiGGer aNTiBody dEpendenT CyTOToxIc ReActIoNS aGainsT tuMor cellS. This propoSal DeScribes an expERiMental paraDigM to identify FC muTationS, cHarACteriZe tHe mutAtions IN functIonal assaYs, ANd ultiMAtely teST ThErapEutic antibodies haRBoRIng the best Fc muTationS iN AnIMAl mOdeL studies. DEsCrIPTIon: AbstraCT TRA

Further research and deve lopment of  thes e F c m ut ants  sho uld lead to th e rape utic antibodies with i ncrea se d  cap a ci ty to  trigge r  a n t ibo dy  d epe nd e nt  cyto tox ic reac tions agai nst  t umor cells.T hi s proposal  de scribes an e xpe riment al  pa r adigm  to  iden tify F c  mutat ions, cha ra c terize the mut a t io ns i n functional assa y s, and ultimately  testth e ra p e uti c a ntibodiesha rbori n g the b e st F c  mu t ations in ani mal model s t udi es. DE SC RIP T ION: A bstra ct Tra

Further_research and_development of these Fc_mutants should_lead_to therapeutic_antibodies_with increased capacity_to trigger antibody_dependent cytotoxic reactions against_tumor cells. This_proposal_describes an experimental paradigm to identify Fc mutations, characterize the mutations in functional assays,_and_ultimately test_therapeutic_antibodies_harboring the best Fc mutations_in animal model studies. DESCRIPTION:_Abstract Tra


, light and ultrastructural microscopical analyses, and computational modeling to address these objectives through the use of physiologically identified nociceptors and anatomically and physiologically identified subsets of second-order spinal interneurons. Detailed knowledge of the cellular mechanisms underlying the normal balance between excitatory and inhibitory interconnections that

, light and ultrastructural microscopical analyses, and computational modeling to cover these aim through the use of physiologically identified nociceptors and anatomically and physiologically identify subset of second - order spinal interneurons. Detailed knowledge of the cellular mechanisms underlie the normal balance between excitatory and inhibitory interconnections that

, lihht and ultrastructural oicroscopical auqlyses, and ckmputatiunal modeling to address thede objextives through the use of physillogicaloy iventified nocice'fors and anatklicanoy and physiolpgically igentified subsatr lf second-order spinal interneurons. Qetailec nnowledge of tre ctlltlar mechanisms underlying the normal galance between exciyatory and inhibitory integconjections that

, light and ultrastructural microscopical analyses, and to these objectives the use of and identified subsets of spinal interneurons. Detailed of the cellular mechanisms underlying the balance between excitatory and inhibitory interconnections that

, light and ultrastructural miCroscopicaL analYseS, anD cOmpuTatiOnal modeling to ADdreSs these objectives throuGh the UsE Of phYSiOlogiCally idENtIFIed NoCiCepToRS aNd anaTomIcally aNd physioloGicAlLy identified SUbSets of secoNd-oRder spinal inTerNeuronS. DEtaILed knOwlEdge oF the ceLLular mEchanisms UnDErlyinG The normAL BaLancE between excitatorY AnD Inhibitory inteRconneCtIOnS THat

, light and ultrastructura l microsco pical  an aly se s, a nd c omputational m o deli ng to address these ob jecti ve s  thr o ug h the  use of ph y s iol og ic all yi de ntifi ednocicep tors and a nat om ically and p h ys iologicall y i dentified su bse ts ofse con d -orde r s pinal  inter n eurons . Detaile dk nowled g e of th e ce llul ar mechanisms und e rl y ing the normal  balan ce be t w een  ex citatory a nd  inhi b itory i n te r c o nne c tions that

, light_and ultrastructural_microscopical analyses, and computational_modeling to_address_these objectives_through_the use of_physiologically identified nociceptors_and anatomically and physiologically_identified subsets of_second-order_spinal interneurons. Detailed knowledge of the cellular mechanisms underlying the normal balance between excitatory_and_inhibitory interconnections_that


perform a series of studies of patients with coronary artery disease, comparing the results of our MRI methods with those of conventional methods used to assess the blood supply to the heart, including radionuclide imaging, CT angiography and conventional cardiac catheterization (including invasive measures of flow reserve). The significance of the proposed work is its potential

perform a series of studies of patients with coronary artery disease, compare the result of our MRI methods with those of conventional methods use to assess the blood provision to the heart, including radionuclide imaging, CT angiography and ceremonious cardiac catheterization (include invasive bill of flow reserve). The significance of the proposed work is its potential

pegform a series of studier of patients wnrh cormnary zrtery dksease, comparing the results od our MRI methods with thosd of convvntional nethies used to assess bke blkld sbp'ly to the hearj, including sadionuclide ikaeiug, CT angiography and conventional cwrdiac vahheterization (ynclldyng jnvasive measures of flow reserve). Fhe sigiificance of thr proposed work is its potfntiwl

perform a series of studies of patients artery comparing the of our MRI methods to assess the supply to the including radionuclide imaging, CT angiography and cardiac catheterization (including invasive measures of flow reserve). The significance of the proposed is its potential

perform a series of studies of Patients wiTh corOnaRy aRtEry dIseaSe, comparing the REsulTs of our MRI methods with tHose oF cONvenTIoNal meThods usED tO ASseSs ThE blOoD SuPply tO thE heart, iNcluding raDioNuClide imaging, ct aNgiography And Conventional CarDiac caThEteRIzatiOn (iNcludIng invASive meAsures of fLoW ReservE). the signIFIcAnce Of the proposed work IS iTS potential

perform a series of studi es of pati entswit h c or onar y ar tery disease,c ompa ring the results of ou r MRI  m e thod s  w ith t hose of co n v ent io na l m et h od s use d t o asses s the bloo d s up ply to the h e ar t, includi ngradionuclide  im aging,  C T a n giogr aph y and  conve n tional  cardiacca t heteri z ation ( i n cl udin g invasive measur e so f flow reserve ). The  s i gn i f ica nce  of the pr op osedw ork isi ts p o ten t ial

perform_a series_of studies of patients_with coronary_artery_disease, comparing_the_results of our_MRI methods with_those of conventional methods_used to assess_the_blood supply to the heart, including radionuclide imaging, CT angiography and conventional cardiac catheterization_(including_invasive measures_of_flow_reserve). The significance of the_proposed work is its potential


sequencing technology to show that the in vivo pattern of nucleosome positioning can be recapitulated in vitro across the S. cerevisiae genome by chromatin remodelers. Here we propose to develop a genome- wide immobilized template assay that will allow us to ask fundamental questions regarding nucleosome dynamics, transcription factor occupancy, and RNA transcription

sequencing technology to show that the in vivo pattern of nucleosome placement can be reprise in vitro across the S. cerevisiae genome by chromatin remodelers. Here we propose to develop a genome- broad trap template assay that will allow us to ask cardinal questions regarding nucleosome dynamics, arrangement factor occupancy, and RNA recording

seeuencing technology to smow that the in ruvo pavtern or nucleorome positioning can be reca'ituoated in vitro across the S. cerevisiwe genomw by xhromatin csmodelevf. Hedc we 'ripose to develpp a genoma- wide immobilhzdd template assay that will allow us eo ask gujdamental quesjions wegadding nucleosome dynamics, transcriltion fector occupancy, and RNA transcription

sequencing technology to show that the in of positioning can recapitulated in vitro by remodelers. Here we to develop a wide immobilized template assay that will us to ask fundamental questions regarding nucleosome dynamics, transcription factor occupancy, and RNA

sequencing technology to shoW that the in Vivo pAttErn Of NuclEosoMe positioning cAN be rEcapitulated in vitro acrOss thE S. CErevISiAe genOme by chROmATIn rEmOdEleRs. hErE we prOpoSe to devElop a genomE- wiDe Immobilized tEMpLate assay tHat Will allow us tO asK fundaMeNtaL QuestIonS regaRding nUCleosoMe dynamicS, tRAnscriPTion facTOR oCcupAncy, and RNA transcrIPtIOn

sequencing technology toshow thatthe i n v ivo  p atte rn o f nucleosome p o siti oning can be recapitul atedin vitr o  a cross  the S. ce r e vis ia egen om e  b y chr oma tin rem odelers. H ere  w e propose to de velop a ge nom e- wide immo bil ized t em pla t e ass aythatwill a l low us  to ask f un d amenta l  questi o n srega rding nucleosomed yn a mics, transcri ptionfa c to r occ upa ncy, and R NA  tran s criptio n

sequencing_technology to_show that the in_vivo pattern_of_nucleosome positioning_can_be recapitulated in_vitro across the_S. cerevisiae genome by_chromatin remodelers. Here_we_propose to develop a genome- wide immobilized template assay that will allow us to_ask_fundamental questions_regarding_nucleosome_dynamics, transcription factor occupancy, and_RNA transcription


of subverting host defense. The goal of this research is to develop a detailed mechanistic understanding of the role of host hemostatic factors in bacterial virulence and the inflammatory response as a step toward novel therapeutic interventions for life-threatening infectious disease. This project will examine how specific neuronal connections are established between vestibular sensory organs and

of subverting host defense. The goal of this inquiry is to originate a detailed mechanistic understanding of the role of server styptic factors in bacterial virulence and the inflammatory response as a tone toward novel therapeutic intervention for life - threatening infectious disease. This undertaking will examine how specific neuronal connections are established between vestibular centripetal organs and

of subverting host defense. The goal of thnw reseerch is to deveuop a detailed mechanistic uiderwtandung of the role of hosg hemostanic factoes ii bacterial virulence and the jkflamketory response ss a step doward novel tvefa'eutic interventions for life-threateging ingeftious disease. Thix pronvcu will examine how specific neurohal coniections are esyablished between vestibulwr sfnsory organs and

of subverting host defense. The goal of is develop a mechanistic understanding of factors bacterial virulence and inflammatory response as step toward novel therapeutic interventions for infectious disease. This project will examine how specific neuronal connections are established between sensory organs and

of subverting host defense. ThE goal of thiS reseArcH is To DeveLop a Detailed mechanIStic Understanding of the role Of hosT hEMostATiC factOrs in baCTeRIAl vIrUlEncE aND tHe infLamMatory rEsponse as a SteP tOward novel thERaPeutic inteRveNtions for lifE-thReatenInG inFEctioUs dIseasE. This pROject wIll examinE hOW speciFIc neuroNAL cOnneCtions are establisHEd BEtween vestibulAr sensOrY OrGANs aNd

of subverting host defens e. The goa l ofthi s r es earc h is  to develop ad etai led mechanistic unders tandi ng of t h eroleof host he m o sta ti cfac to r sin ba cte rial vi rulence an d t he  inflammator y  r esponse as  astep towardnov el the ra peu t ic in ter venti ons fo r  life- threateni ng infect i ous dis e a se . Th is project will e x am i ne how specifi c neur on a lc o nne cti ons are es ta blish e d betwe e nv e s tib u lar sensory o rgans and

of_subverting host_defense. The goal of_this research_is_to develop_a_detailed mechanistic understanding_of the role_of host hemostatic factors_in bacterial virulence_and_the inflammatory response as a step toward novel therapeutic interventions for life-threatening infectious disease._This_project will_examine_how_specific neuronal connections are established_between vestibular sensory organs and


aggregation follows a seeding-nucleation mechanism and involves several intermediates, including soluble oligomers and protofibrils. Recent evidence has shown that tau oligomers are circulating in biological fluids and these structures appear to be key for inducing brain degeneration in AD. Our working hypothesis is that detection of misfolded tau oligomers circulating

aggregation follows a seeding - nucleation mechanism and involves several intermediate, include soluble oligomers and protofibrils. Recent evidence has shown that tau oligomers are circulate in biological fluid and these structures appear to be cardinal for inducing brain degeneration in AD. Our ferment hypothesis is that detection of misfolded tau oligomers circulate

aghregation follows a seedlng-nucleation meeyanism and ihvolves reveral intermediates, includmng woluboe oligomers and protowibrils. Rvcent evieenct has shown that vzu oligomers zve cixcnlating in biolpgical fluhds and these vtfuetures appear to be key for inducing brain ceheneration in WD. Olr woripnn hypothesis is that detection or misfonded tau oligpmers circulating

aggregation follows a seeding-nucleation mechanism and involves including oligomers and Recent evidence has circulating biological fluids and structures appear to key for inducing brain degeneration in Our working hypothesis is that detection of misfolded tau oligomers circulating

aggregation follows a seedinG-nucleatioN mechAniSm aNd InvoLves Several intermeDIateS, including soluble oligoMers aNd PRotoFIbRils. REcent evIDeNCE haS sHoWn tHaT TaU oligOmeRs are ciRculating iN biOlOgical fluids ANd These strucTurEs appear to be Key For indUcIng BRain dEgeNeratIon in Ad. our worKing hypotHeSIs is thAT detectION oF misFolded tau oligomerS CiRCulating

aggregation follows a see ding-nucle ation  me cha ni sm a nd i nvolves severa l  int ermediates, includingsolub le olig o me rs an d proto f ib r i ls.  R ec ent  e v id encehas  shownthat tau o lig om ers are circ u la ting in bi olo gical fluids  an d thes estr u cture s a ppear  to be key fo r inducin gb rain d e generat i o nin A D. Our working hy p ot h esis is that d etecti on of m isf old ed tau oli go mersc irculat i ng

aggregation_follows a_seeding-nucleation mechanism and involves_several intermediates,_including_soluble oligomers_and_protofibrils. Recent evidence_has shown that_tau oligomers are circulating_in biological fluids_and_these structures appear to be key for inducing brain degeneration in AD. Our working_hypothesis_is that_detection_of_misfolded tau oligomers circulating


utilization, and metabolic health. The role of extragonadal estrogen production in obesity's effects will be dissected with the use of aromatase inhibitors. Diet-induced weight loss is common in women with weight control problems, but the body compensates with homeostatic adaptations that drive weight regain. The loss of ovarian function in weight

utilization, and metabolic health. The role of extragonadal estrogen production in obesity's effect will be dissect with the use of aromatase inhibitors. Diet - induced weight unit loss is common in womanhood with weight unit control problem, but the body compensates with homeostatic adaptation that drive weight regain. The personnel casualty of ovarian function in weight

utllization, and metabolic mealth. The role of extregonadam estrogdn production in obesity's efhectw wilo be dissected with thd use of wromatasw iniibitors. Diet-indnded weinkt load is rommon in women with weigvt control protldmd, but the body compensates with homqostativ wdaptations thwt dgide wspgmt regain. The loss of ovarian fuhction pn weight

utilization, and metabolic health. The role of production obesity's effects be dissected with Diet-induced loss is common women with weight problems, but the body compensates with adaptations that drive weight regain. The loss of ovarian function in weight

utilization, and metabolic heAlth. The rolE of exTraGonAdAl esTrogEn production in OBesiTy's effects will be dissecTed wiTh THe usE Of AromaTase inhIBiTORs. DIeT-iNduCeD WeIght lOss Is commoN in women wiTh wEiGht control prOBlEms, but the bOdy Compensates wIth HomeosTaTic ADaptaTioNs thaT drive WEight rEgain. The lOsS Of ovarIAn functION iN weiGht

utilization, and metaboli c health.The r ole  of  e xtra gona dal estrogen p r oduc tion in obesity's effe cts w il l  bed is secte d witht he u seof  a rom at a se  inhi bit ors. Di et-induced  we ig ht loss is c o mm on in wome n w ith weight c ont rol pr ob lem s , but  th e bod y comp e nsates  with hom eo s tatica daptati o n sthat  drive weight reg a in .  The loss of o varian  f u nc t i oninweight

utilization,_and metabolic_health. The role of_extragonadal estrogen_production_in obesity's_effects_will be dissected_with the use_of aromatase inhibitors. Diet-induced_weight loss is_common_in women with weight control problems, but the body compensates with homeostatic adaptations that_drive_weight regain._The_loss_of ovarian function in weight