ers of the coat protein, a feature common among the inner protein shells of dsRNA viruses, and probably related to their unique mode of transcription and replication. The two identical Gag molecules in each dimer are in non-equivalent environments, and show substantially different conformations in specific surface regions. This virus decaps cellular mRNA

ers of the coat protein, a feature common among the inside protein shell of dsRNA viruses, and credibly related to their singular mode of transcription and reproduction. The two identical Gag molecules in each dimer are in non - equivalent environments, and prove substantially different conformity in specific surface regions. This virus decaps cellular messenger rna

ers of the coat protein, a ftature common amout the mnner pdotein sfells of dsRNA viruses, and pcobavly rtjated to their unidue mode lf transxripuion and replicatmkn. The bco idsktican Gag molecules in each dhmer are in not-edunvalent environments, and show substagtially dlfferent confotmatipgs ih specific surface regions. This vidus deceps cellular mRMA

ers of the coat protein, a feature the protein shells dsRNA viruses, and mode transcription and replication. two identical Gag in each dimer are in non-equivalent and show substantially different conformations in specific surface regions. This virus decaps cellular

ers of the coat protein, a featuRe common amOng thE inNer PrOteiN sheLls of dsRNA viruSEs, anD probably related to theiR uniqUe MOde oF TrAnscrIption aND rEPLicAtIoN. ThE tWO iDentiCal gag moleCules in eacH diMeR are in non-equIVaLent enviroNmeNts, and show suBstAntialLy DifFErent ConFormaTions iN SpecifIc surface ReGIons. ThIS virus dECApS celLular mRNA

ers of the coat protein, a  feature c ommon  am ong  t he i nner  protein shell s  ofdsRNA viruses, and pro bably  r e late d  t o the ir uniq u em o deof  t ran sc r ip tionand  replic ation. The  tw oidentical Ga g  m olecules i n e ach dimer ar e i n non- eq uiv a lentenv ironm ents,a nd sho w substan ti a lly di f ferentc o nf orma tions in specific su r face regions.This v ir u sd e cap s c ellular mR NA

ers of_the coat_protein, a feature common_among the_inner_protein shells_of_dsRNA viruses, and_probably related to_their unique mode of_transcription and replication._The_two identical Gag molecules in each dimer are in non-equivalent environments, and show substantially_different_conformations in_specific_surface_regions. This virus decaps cellular_mRNA


global approach enabling study of many different aspects of action of antimicrobial peptides at once. In this approach altered susceptibilities to the peptide of thousands deletion mutants of yeast Saccharomyces cerevisiae were tested simultaneously. Among identified deletions conferring hypersensitivity to the peptide, many involved genes associated with the RIM101 signaling pathway, best known as

global approach enabling study of many unlike view of action of antimicrobial peptides at once. In this overture change susceptibilities to the peptide of thousands omission mutants of yeast Saccharomyces cerevisiae were tested simultaneously. Among identified omission conferring hypersensitivity to the peptide, many involved gene associated with the RIM101 signaling pathway, best known as

gllbal approach enabling suudy of many diffgrwnt as'ects or action of antimicrobial peptides av onxe. In this approach altered susceptihilities to uhe peptide of thousands deletikk mutcnvs of yeast Sacgharomyces werevisiae wera gedted simultaneously. Among identifieq deletoojs conferring rypegsqnsifpvlty to the peptide, many involved genes essociated with the RIM101 signaling pathway, besh known as

global approach enabling study of many different action antimicrobial peptides once. In this peptide thousands deletion mutants yeast Saccharomyces cerevisiae tested simultaneously. Among identified deletions conferring to the peptide, many involved genes associated with the RIM101 signaling pathway, best as

global approach enabling stuDy of many diFfereNt aSpeCtS of aCtioN of antimicrobiAL pepTides at once. In this approAch alTeREd suSCePtibiLities tO ThE PEptIdE oF thOuSAnDs delEtiOn mutanTs of yeast SAccHaRomyces cerevISiAe were testEd sImultaneouslY. AmOng ideNtIfiED deleTioNs conFerrinG HypersEnsitivitY tO The pepTIde, many INVoLved Genes associated wiTH tHE RIM101 signaling pAthway, BeST kNOWn aS

global approach enablingstudy of m any d iff ere nt  asp ects  of action ofa ntim icrobial peptides at o nce.In this ap proac h alter e ds u sce pt ib ili ti e sto th e p eptideof thousan dsde letion mutan t sof yeast S acc haromyces ce rev isiaewe ret ested  si multa neousl y . Amon g identif ie d  delet i ons con f e rr inghypersensitivityt ot he peptide, ma ny inv ol v ed g ene s a ssociatedwi th th e  RIM101 si g n a lin g  pathway, bes t known as

global_approach enabling_study of many different_aspects of_action_of antimicrobial_peptides_at once. In_this approach altered_susceptibilities to the peptide_of thousands deletion_mutants_of yeast Saccharomyces cerevisiae were tested simultaneously. Among identified deletions conferring hypersensitivity to the_peptide,_many involved_genes_associated_with the RIM101 signaling pathway,_best known as


specific tumors that exhibited high NCTX uptake as visualized by X-ray imaging were the most susceptible to treatment by SLD. There are currently 128 ongoing clinical trials in the US (www.ClinicalTrials.gov), of Stealth Liposomal Doxorubicin (Doxil(r) and various generic equivalence

specific tumors that exhibited high NCTX uptake as visualize by ten - ray imaging were the most susceptible to discussion by SLD. There are currently 128 ongoing clinical trials in the US (www.ClinicalTrials.gov), of Stealth Liposomal Doxorubicin (Doxil(r) and assorted generic equivalence

spfcific tumors that exhiblted high NCTX uktqke as visuamized by X-ray imaging were the most duwceptuble to treatment by SUD. There wre currwntlb 128 ongoing clinirzl trials in fme US (xww.ClinicalTriaks.gov), of Sdealth Liposomdl Dlxorubicin (Doxil(r) and various generyc equifapence

specific tumors that exhibited high NCTX uptake by imaging were most susceptible to currently ongoing clinical trials the US (www.ClinicalTrials.gov), Stealth Liposomal Doxorubicin (Doxil(r) and various equivalence

specific tumors that exhibitEd high NCTX UptakE as VisUaLizeD by X-Ray imaging were THe moSt susceptible to treatmeNt by SlD. tHere ARe CurreNtly 128 ongOInG CLinIcAl TriAlS In The US (Www.clinicaLTrials.gov), Of STeAlth LiposomaL doXorubicin (DOxiL(r) and various GenEric eqUiValENce

specific tumors that exhi bited high  NCTX  up tak eas v isua lized by X-ray imag ing were the most susc eptib le to t r ea tment  by SLD .  T h e rear ecur re n tl y 128  on going c linical tr ial sin the US (w w w. ClinicalTr ial s.gov), of S tea lth Li po som a l Dox oru bicin  (Doxi l (r) an d various  g e nerice quivale n c e

specific_tumors that_exhibited high NCTX uptake_as visualized_by_X-ray imaging_were_the most susceptible_to treatment by_SLD. There are currently_128 ongoing clinical_trials_in the US (www.ClinicalTrials.gov), of Stealth Liposomal Doxorubicin (Doxil(r) and various generic equivalence


the program address specific basic problems in biomedical sciences, many with direct applications to human disease. Flow Cytometry Project Summary/Abstract The primary purpose of the Flow Cytometry Module is to provide Principal Investigators with a centralized service for cell phenotyping, sorting (FACS) to obtain viable populations of cells according to specific cellular

the program address specific basic problems in biomedical sciences, many with lineal application to human disease. Flow Cytometry Project Summary / Abstract The primary function of the Flow Cytometry Module is to put up Principal Investigators with a centralized service for cell phenotyping, classify (FACS) to prevail viable population of cells according to specific cellular

thf program address speciflc basic problems in bimmediczl scienzes, many with direct applicavionw to yuman disease. Flow Cytumetry Prlject Sunmarb/Abstract The prmjary puv'ose kn the Hlow Cytometry Kodule is do provide Pritckpcl Investigators with a centralized fervice flr cell phenotiping, fortjng (FACS) to obtain viable populatikns of bells according tp specific cellular

the program address specific basic problems in many direct applications human disease. Flow purpose the Flow Cytometry is to provide Investigators with a centralized service for phenotyping, sorting (FACS) to obtain viable populations of cells according to specific cellular

the program address specific Basic problEms in BioMedIcAl scIencEs, many with direCT appLications to human diseasE. Flow cyTOmetRY PRojecT SummarY/abSTRacT THe PriMaRY pUrposE of The Flow cytometry MOduLe Is to provide PRInCipal InvesTigAtors with a ceNtrAlized SeRviCE for cEll PhenoTyping, SOrting (fACS) to obtAiN Viable POpulatiONS oF celLs according to specIFiC Cellular

the program address speci fic basicprobl ems  in  b iome dica l sciences, ma n y wi th direct applications  to h um a n di s ea se. F low Cyt o me t r y P ro je ctSu m ma ry/Ab str act The  primary p urp os e of the Flo w  C ytometry M odu le is to pro vid e Prin ci pal Inves tig ators  witha  centr alized se rv i ce for cell ph e n ot ypin g, sorting (FACS) to obtain viablepopula ti o ns o f c ell s accordin gto sp e cific c e ll u l a r

the_program address_specific basic problems in_biomedical sciences,_many_with direct_applications_to human disease._Flow Cytometry Project_Summary/Abstract The primary purpose_of the Flow_Cytometry_Module is to provide Principal Investigators with a centralized service for cell phenotyping, sorting_(FACS)_to obtain_viable_populations_of cells according to specific_cellular


informatics systems, research networking platforms and training programs to promote multi-disciplinary team science; and creation and leadership of a regional consortium of the three Chicago-area CTSAs to promote community engagement activities and practice-based research. In the present proposal, we describe our second generation institute, NUCATS 2.0

informatics systems, research networking platforms and education broadcast to promote multi - disciplinary team skill; and creation and leadership of a regional consortium of the three Chicago - sphere CTSAs to promote community employment activity and practice - establish research. In the present proposal, we identify our second generation institute, NUCATS 2.0

invormatics systems, researgh networking plcrforms and tdaining orograms to promote multi-disriplunary team science; and creagion and peadershup oh a regional consortium of the bhree Rhicago-area CTSSs to prommte community aneayement activities and practice-based wesearcn. Ln the present prokoswl, ws describe our second generation ihstitutt, NUCATS 2.0

informatics systems, research networking platforms and training promote team science; creation and leadership the Chicago-area CTSAs to community engagement activities practice-based research. In the present proposal, describe our second generation institute, NUCATS 2.0

informatics systems, researcH networkinG platForMs aNd TraiNing Programs to promOTe muLti-disciplinary team sciEnce; aNd CReatIOn And leAdershiP Of A REgiOnAl ConSoRTiUm of tHe tHree ChiCago-area CTsAs To Promote commuNItY engagemenT acTivities and pRacTice-baSeD reSEarch. in tHe preSent prOPosal, wE describe OuR Second GEneratiON InStitUte, NUCATS 2.0

informatics systems, rese arch netwo rking  pl atf or ms a nd t raining progra m s to  promote multi-discipl inary  t e am s c ie nce;and cre a ti o n  an dle ade rs h ip  of a  re gionalconsortium  of  t he three Chi c ag o-area CTS Asto promote c omm unityen gag e mentact iviti es and practi ce-basedre s earch. In thep r es entproposal, we desc r ib e  our second ge nerati on in s t itu te,  NUCATS 2. 0

informatics_systems, research_networking platforms and training_programs to_promote_multi-disciplinary team_science;_and creation and_leadership of a_regional consortium of the_three Chicago-area CTSAs_to_promote community engagement activities and practice-based research. In the present proposal, we describe our_second_generation institute,_NUCATS_2.0


students will also be required to attend a short course in the Responsible Conduct of Research. A plan for evaluation of program effectiveness is proposed that will assess career goals, perceived barriers, knowledge of academic research careers, and knowledge of higher education in research. The evaluation plan will be used as the basis of a report, after

students will also be required to attend a light path in the Responsible Conduct of Research. A plan for evaluation of program effectiveness is project that will assess career goals, perceive barrier, knowledge of academic research careers, and knowledge of high education in research. The evaluation design will be used as the basis of a report, after

stkdents will also be requlred to attend a short rourse jn the Rdsponsible Conduct of Researrh. A plan for evaluation of proeram effebtiveness is kroposed that will assess careed goanw, perceived batriers, knowladge of academhc rzsearch careers, and knowledge of higrer eduvahion in researsh. Tnq evzluation plan will be used as the gasis oh a report, aftet

students will also be required to attend course the Responsible of Research. A effectiveness proposed that will career goals, perceived knowledge of academic research careers, and of higher education in research. The evaluation plan will be used as the of a report, after

students will also be requireD to attend a Short CouRse In The REspoNsible Conduct oF reseArch. A plan for evaluation Of proGrAM effECtIveneSs is proPOsED ThaT wIlL asSeSS cAreer GoaLs, perceIved barrieRs, kNoWledge of acadEMiC research cAreErs, and knowleDge Of highEr EduCAtion In rEsearCh. The eVAluatiOn plan wilL bE Used as THe basis OF A rEporT, after

students will also be req uired to a ttend  asho rt  cou rsein the Respons i bleConduct of Research. A  plan  f o r ev a lu ation  of pro g ra m eff ec ti ven es s  i s pro pos ed that  will asse ssca reer goals,p er ceived bar rie rs, knowledg e o f acad em icr esear chcaree rs, an d  knowl edge of h ig h er edu c ation i n re sear ch. The evaluatio n  p l an will be use d as t he ba s i s o f a  report, a ft er

students_will also_be required to attend_a short_course_in the_Responsible_Conduct of Research._A plan for_evaluation of program effectiveness_is proposed that_will_assess career goals, perceived barriers, knowledge of academic research careers, and knowledge of higher_education_in research._The_evaluation_plan will be used as_the basis of a report,_after


, and 60 months of age. The Durham Child Health and Development Study has demographic, maternal report, behavioral, and physiological data on 199 infants and their families at seven time points (3, 6, 12, 18, 24, 30, and 36 months). At each visit, infant vagal tone and behavioral response was

, and 60 months of age. The Durham Child Health and Development Study has demographic, maternal reputation, behavioral, and physiologic data on 199 infants and their class at seven fourth dimension points (3, 6, 12, 18, 24, 30, and 36 months). At each sojourn, infant vagal tone and behavioral response was

, anf 60 months of age. The Durmam Child Health and Detelopmeht Study has demographic, maternal re'ort, behacioral, and physiologicxl data oj 199 infanrs aid their families at sevcu tims poiutw (3, 6, 12, 18, 24, 30, and 36 mpnths). At edch visit, infatt vcgal tone and behavioral response waf

, and 60 months of age. The Health Development Study demographic, maternal report, 199 and their families seven time points 6, 12, 18, 24, 30, and months). At each visit, infant vagal tone and behavioral response was

, and 60 months of age. The Durham ChIld Health aNd DevEloPmeNt studY has Demographic, matERnal Report, behavioral, and phySioloGiCAl daTA oN 199 infaNts and tHEiR FAmiLiEs At sEvEN tIme poIntS (3, 6, 12, 18, 24, 30, and 36 monThs). At each vIsiT, iNfant vagal toNE aNd behaviorAl rEsponse was

, and 60 months of age. Th e Durham C hildHea lth  a nd D evel opment Study h a s de mographic, maternal re port,  b e havi o ra l, an d physi o lo g i cal  d at a o n1 99  infa nts  and th eir famili esat  seven timep oi nts (3, 6,  12 , 18, 24, 30 , a nd 36mo nth s ). At  ea ch vi sit, i n fant v agal tone  a n d beha v ioral r e s po nsewas

, and_60 months_of age. The Durham_Child Health_and_Development Study_has_demographic, maternal report,_behavioral, and physiological_data on 199 infants_and their families_at_seven time points (3, 6, 12, 18, 24, 30, and 36 months). At each_visit,_infant vagal_tone_and_behavioral response was


interstitial cystitis, non-cardiac chest pain and fibromyalgia. PUBLIC HEALTH RELEVANCE: In the current proposal, we have developed the concept that chronic stress may induce activation of the immune system in the spinal cord, initiating a cascade of events that will affect the sensory system. This new approach has

interstitial cystitis, non - cardiac chest pain and fibromyalgia. PUBLIC HEALTH RELEVANCE: In the current proposal, we have developed the concept that chronic tension may induce energizing of the immune organization in the spinal cord, initiating a shower of events that will feign the sensory system. This fresh approach has

inherstitial cystitis, non-cxrdiac chest panb and hibromyzlgia. PUCLIC HEALTH RELEVANCE: In the cyrrenu proposal, we have aeveloped the conxept rhat chronmd stress may jkduce ectivation of tme immune sfstem in the s[ivap cord, initiating a cascade of evenes that wlll affect the senxjry ajsuem. This new approach has

interstitial cystitis, non-cardiac chest pain and fibromyalgia. RELEVANCE: the current we have developed may activation of the system in the cord, initiating a cascade of events will affect the sensory system. This new approach has

interstitial cystitis, non-caRdiac chest Pain aNd fIbrOmYalgIa. PUbLIC HEALTH RELEvaNCE: in the current proposal, we Have dEvELopeD ThE concEpt that CHrONIc sTrEsS maY iNDuCe actIvaTion of tHe immune sySteM iN the spinal coRD, iNitiating a CasCade of events ThaT will aFfEct THe senSorY systEm. This NEw apprOach has

interstitial cystitis, no n-cardiacchest  pa inan d fi brom yalgia. PUBLIC HEAL TH RELEVANCE: In the c urren tp ropo s al , wehave de v el o p edth econ ce p tthatchr onic st ress may i ndu ce  activationo fthe immune  sy stem in thespi nal co rd , i n itiat ing  a ca scadeo f even ts that w il l  affec t  the se n s or y sy stem. This new ap p ro a ch has

interstitial_cystitis, non-cardiac_chest pain and fibromyalgia._PUBLIC HEALTH_RELEVANCE:_In the_current_proposal, we have_developed the concept_that chronic stress may_induce activation of_the_immune system in the spinal cord, initiating a cascade of events that will affect_the_sensory system._This_new_approach has


, Curriculum Committee and Trainee Review Committees. Participating faculty are selected on the basis of research interest in Pharmacology and enthusiasm for participating in Program activities including advising Ph.D. students, participation on student committees, classroom teaching, student advising and survival skills mentoring. The training includes coursework in four

, Curriculum Committee and Trainee Review Committees. Participating faculty are selected on the basis of inquiry sake in Pharmacology and enthusiasm for participating in Program bodily process include advising Ph.D. scholar, engagement on student committees, classroom teaching, student advising and survival skills mentor. The training includes coursework in four

, Cugriculum Committee and Tvainee Review Committeev. Partjcipatine faculty are selected on thx bawis od research interest in Pharmacopogy and entiusiasm for partmdipatinn in Lvograk activities ingluding advhsing Ph.D. studangs, participation on student committeef, classtolm teaching, stodent wdviapnn and survival skills mentoring. Fhe trapning includes coirsework in four

, Curriculum Committee and Trainee Review Committees. are on the of research interest participating Program activities including Ph.D. students, participation student committees, classroom teaching, student advising survival skills mentoring. The training includes coursework in four

, Curriculum Committee and TraInee Review commiTteEs. PArTiciPatiNg faculty are seLEcteD on the basis of research iNtereSt IN PhaRMaCologY and entHUsIASm fOr PaRtiCiPAtIng in proGram actIvities incLudInG advising Ph.D. STuDents, partiCipAtion on studeNt cOmmittEeS, clASsrooM teAchinG, studeNT advisIng and surViVAl skilLS mentorING. THe trAining includes couRSeWOrk in four

, Curriculum Committee and  Trainee R eview  Co mmi tt ees.  Par ticipating fac u ltyare selected on the ba sis o fr esea r ch  inte rest in Ph a r mac ol og y a nd en thusi asm  for pa rticipatin g i nProgram acti v it ies includ ing  advising Ph .D.  stude nt s,p artic ipa tionon stu d ent co mmittees,  c l assroo m  teachi n g ,stud ent advising ands ur v ival skills me ntorin g. Th e tra ini ng include scours e work in fo u r

, Curriculum_Committee and_Trainee Review Committees. Participating_faculty are_selected_on the_basis_of research interest_in Pharmacology and_enthusiasm for participating in_Program activities including_advising_Ph.D. students, participation on student committees, classroom teaching, student advising and survival skills mentoring._The_training includes_coursework_in_four


iroheme is a cofactor in the synthesis of cysteine. Heme plays a role in both aerobic and anaerobic respiration, and it has been found that 02 may regulate the production of ALA. These considerations suggest that the control of this pathway requires interesting strategies which are worth investigating. The goal of this project is to

iroheme is a cofactor in the synthesis of cysteine. Heme plays a role in both aerobic and anaerobic respiration, and it has been find oneself that 02 may determine the production of ALA. These considerations suggest that the dominance of this pathway requires interesting strategy which are deserving investigating. The goal of this project is to

irojeme is a cofactor in tht synthesis of cysteine. Ieme plzys a roue in both aerobic and anaerlbuc rewpiration, and it has bden found that 02 mqy rtgulate the produrfion of ALA. Tgcse cmisiderations sungest that dhe control of tfid pathway requires interesting straeegies ehlch are worth ynvexeigafpnn. The goal of this project is to

iroheme is a cofactor in the synthesis Heme a role both aerobic and been that 02 may the production of These considerations suggest that the control this pathway requires interesting strategies which are worth investigating. The goal of this is to

iroheme is a cofactor in the syNthesis of cYsteiNe. HEme PlAys a Role In both aerobic aND anaErobic respiration, and it Has beEn FOund THaT 02 may rEgulate THe PROduCtIoN of aLa. thEse coNsiDeratioNs suggest tHat ThE control of thIS pAthway requIreS interesting StrAtegieS wHicH Are woRth InvesTigatiNG. The goAl of this pRoJEct is tO

iroheme is a cofactor in t he synthes is of  cy ste in e. H emeplays a role i n  bot h aerobic and anaerobi c res pi r atio n ,and i t has b e en f oun dth at02 ma y reg ula te theproduction  of  A LA. These co n si derationssug gest that th e c ontrol  o f t h is pa thw ay re quires intere sting str at e gies w h ich are w or th i nvestigating. The go a l of this proj ect is  t o

iroheme is_a cofactor_in the synthesis of_cysteine. Heme_plays_a role_in_both aerobic and_anaerobic respiration, and_it has been found_that 02 may_regulate_the production of ALA. These considerations suggest that the control of this pathway requires_interesting_strategies which_are_worth_investigating. The goal of this_project is to


provide technical support in the analysis of study drugs. Research: In the last 10 years, patients have markedly increased their use of dietary supplements to treat and prevent chronic medical conditions like atherosclerotic cardiovascular disease. This widespread use continues despite little scientific evidence of benefit from randomized controlled trials (RCTs). Current estimates suggest that 30 percent

provide technical support in the analysis of study drug. inquiry: In the concluding 10 years, patients have markedly increase their use of dietary supplements to process and prevent chronic medical conditions like atherosclerotic cardiovascular disease. This far-flung habit continues despite short scientific evidence of benefit from randomized controlled trial (RCTs). Current estimates indicate that 30 percent

prlvide technical support ln the analysis of studb drugs. Researcf: In the last 10 years, patientd yave narkedly increased thekr use of dietary supklements to treat and preyznt cgvonic nedical conditlons like adherosclerotic cxrbiovascular disease. This widespread tse conyijues despite lyttlt ssienfpflc evidence of benefit from randkmized bontrolled trials (RCTs). Current estimates suhgesh that 30 percent

provide technical support in the analysis of Research: the last years, patients have dietary to treat and chronic medical conditions atherosclerotic cardiovascular disease. This widespread use despite little scientific evidence of benefit from randomized controlled trials (RCTs). Current estimates that 30 percent

provide technical support in The analysiS of stUdy DruGs. reseArch: in the last 10 years, PAtieNts have markedly increasEd theIr USe of DIeTary sUpplemeNTs TO TreAt AnD prEvENt ChronIc mEdical cOnditions lIke AtHerosclerotiC CaRdiovasculAr dIsease. This wiDesPread uSe ConTInues DesPite lIttle sCIentifIc evidencE oF BenefiT From ranDOMiZed cOntrolled trials (RCtS). CURrent estimates SuggesT tHAt 30 PERceNt

provide technical support  in the an alysi s o f s tu dy d rugs . Research: In thelast 10 years, patient s hav em arke d ly  incr eased t h ei r use  o fdie ta r ysuppl eme nts totreat andpre ve nt chronic m e di cal condit ion s like ather osc leroti ccar d iovas cul ar di sease. This w idespread  u s e cont i nues de s p it e li ttle scientific e v id e nce of benefit  fromra n do m i zed  co ntrolled t ri als ( R CTs). C u rr e n t  es t imates sugges t that 30 p e rce nt

provide_technical support_in the analysis of_study drugs._Research:_In the_last_10 years, patients_have markedly increased_their use of dietary_supplements to treat_and_prevent chronic medical conditions like atherosclerotic cardiovascular disease. This widespread use continues despite little_scientific_evidence of_benefit_from_randomized controlled trials (RCTs). Current_estimates suggest that 30 percent


reflect changes in protein secretion and shedding from renal tubular cells and other sources. We predict that these changes will vary with onset, duration and severity of the obstruction. In our previous work, we have demonstrated our ability to use state-of-the-art mass spectrometric and proteomic approaches to identify proteins in the urinary

reflect changes in protein secretion and shedding from renal tubular cell and early sources. We predict that these change will deviate with onset, duration and severity of the obstacle. In our previous work, we have demonstrated our ability to practice department of state - of - the - art bulk spectrometric and proteomic approaches to identify protein in the urinary

revlect changes in protein secretion and sheddinj from denal tucular cells and other sourced. Qe prtbict that these chanees will nary with onstt, duration and sxberity of the lbstxurtion. In our prgvious work, fe have demonsdrxtzd our ability to use state-of-the-art iass sprchrometric and krotepiic zipvoaches to identify proteins in fhe uriiary

reflect changes in protein secretion and shedding tubular and other We predict that onset, and severity of obstruction. In our work, we have demonstrated our ability use state-of-the-art mass spectrometric and proteomic approaches to identify proteins in the urinary

reflect changes in protein seCretion and SheddIng FroM rEnal TubuLar cells and othER souRces. We predict that these ChangEs WIll vARy With oNset, durATiON And SeVeRitY oF ThE obstRucTion. In oUr previous WorK, wE have demonstRAtEd our abiliTy tO use state-of-tHe-aRt mass SpEctROmetrIc aNd proTeomic APproacHes to idenTiFY proteINs in the URInAry

reflect changes in protei n secretio n and  sh edd in g fr om r enal tubular c e llsand other sources. Wepredi ct that th ese c hangesw il l var ywi thon s et , dur ati on andseverity o f t he  obstruction .  I n our prev iou s work, we h ave  demon st rat e d our  ab ility  to us e  state -of-the-a rt mass s p ectrome t r ic  and  proteomic approa c he s  to identify p rotein si nt h e u rin ary

reflect_changes in_protein secretion and shedding_from renal_tubular_cells and_other_sources. We predict_that these changes_will vary with onset,_duration and severity_of_the obstruction. In our previous work, we have demonstrated our ability to use state-of-the-art_mass_spectrometric and_proteomic_approaches_to identify proteins in the_urinary


particularly as correlated with tumor responsiveness to antiestrogen therapy. The results should help improve the design of atiestrogen therapy alone or in combination with chemotherapy, should aid in the search for more effective antiestrogens, and should also contribute substantially to knowledge of normal estrogen action and the nature of hormone dependence in breast cancer.

particularly as correlated with tumor responsiveness to antiestrogen therapy. The results should help better the invention of atiestrogen therapy alone or in combination with chemotherapy, should aid in the search for more effective antiestrogens, and should also contribute well to knowledge of normal estrogen action and the nature of hormone dependence in breast cancer.

pagticularly as correlated with tumor reskobsivenxss to zntiestrugen therapy. The results shonld yelp umprove the design of xtiestrogvn therapt alibe or in combination witg chekitherapy, shoulc aid in tve search for kofe effective antiestrogens, and should also cpnhribute substagtiakjy tk knowledge of normal estrogen actjon and the nature og hormone dependence in brfast cancer.

particularly as correlated with tumor responsiveness to The should help the design of combination chemotherapy, should aid the search for effective antiestrogens, and should also contribute to knowledge of normal estrogen action and the nature of hormone dependence in cancer.

particularly as correlated wIth tumor reSponsIveNesS tO antIestRogen therapy. ThE ResuLts should help improve thE desiGn OF atiEStRogen Therapy ALoNE Or iN cOmBinAtIOn With cHemOtherapY, should aid In tHe Search for morE EfFective antIesTrogens, and shOulD also cOnTriBUte suBstAntiaLly to kNOwledgE of normal EsTRogen aCTion and THE nAturE of hormone dependeNCe IN breast cancer.

particularly as correlate d with tum or re spo nsi ve ness  toantiestrogen t h erap y. The results shouldhelpim p rove th e des ign ofa ti e s tro ge nthe ra p yalone  or  in com bination w ith  c hemotherapy, sh ould aid i n t he search fo r m ore ef fe cti v e ant ies troge ns, an d  shoul d also co nt r ibutes ubstant i a ll y to  knowledge of nor m al estrogen actio n andth e  n a t ure  of  hormone d ep enden c e in br e as t c anc e r.

particularly_as correlated_with tumor responsiveness to_antiestrogen therapy._The_results should_help_improve the design_of atiestrogen therapy_alone or in combination_with chemotherapy, should_aid_in the search for more effective antiestrogens, and should also contribute substantially to knowledge_of_normal estrogen_action_and_the nature of hormone dependence_in breast cancer.


I) now allow detection not only of infarcted brain but also of brain that is potentially salvageable if blood flow can be restored. MR DWI is the gold standard in medical imaging for identifying irreversibly infarcted brain (core infarct). Brain can progress from reversible but severe ischemia to irreversible infarction over the matter

I) now allow detection not only of infarcted mind but besides of brain that is potentially salvageable if blood menstruation can be regenerate. MR DWI is the gold standard in aesculapian imaging for identifying irreversibly infarcted brain (kernel infarct). Brain can progress from reversible but dangerous ischemia to irreversible infarction over the matter

I) nlw allow detection not okly of infarcted brain uut alsk of brakn that is potentially salvajeaboe if blood flow can be resgored. MR FWI is tye giod standarv in medleal ijwginy hor identifying irreversitly infarcted trxiu (core infarct). Brain can progress frjm revetslble but severg iscnqmia no irreversible infarction over the mauter

I) now allow detection not only of but of brain is potentially salvageable restored. DWI is the standard in medical for identifying irreversibly infarcted brain (core Brain can progress from reversible but severe ischemia to irreversible infarction over the

I) now allow detection not only Of infarcteD braiN buT alSo Of brAin tHat is potentialLY salVageable if blood flow can Be resToREd. MR dwI Is the Gold staNDaRD In mEdIcAl iMaGInG for iDenTifying IrreversibLy iNfArcted brain (cORe Infarct). BraIn cAn progress frOm rEversiBlE buT SeverE isChemiA to irrEVersibLe infarctIoN Over thE Matter

I) now allow detection not  only of i nfarc ted  br ai n bu t al so of brain th a t is  potentially salvageab le if  b l oodf lo w can  be res t or e d . M RDW I i st he  gold  st andardin medical  im ag ing for iden t if ying irrev ers ibly infarct edbrain(c ore infar ct) . Bra in can progre ss from r ev e rsible but sev e r eisch emia to irreversi b le infarction ove r thema t te r

I) now_allow detection_not only of infarcted_brain but_also_of brain_that_is potentially salvageable_if blood flow_can be restored. MR_DWI is the_gold_standard in medical imaging for identifying irreversibly infarcted brain (core infarct). Brain can progress_from_reversible but_severe_ischemia_to irreversible infarction over the_matter


surface receptors during normal human myeloid differentiation. These membrane markers, now being used to examine leukemic myeloid differentiation are detected on cells from only a minority of patients. The objectives of this proposed research are: (1) to induce differentiation in freshly isolated human acute nonlymphoid leukemic cells (ANLL) by incubation with

surface receptors during normal human myeloid differentiation. These membrane markers, nowadays being use to examine leukemic myeloid differentiation are detected on cell from only a minority of patients. The objective of this nominate research are: (1) to induce specialization in newly isolated human acute accent nonlymphoid leukemic cells (ANLL) by incubation with

sugface receptors during nurmal human myeloid dihferentjation. Tfese membrane markers, now bemng ysed uj examine leukemic myeloid fifferenriatmon are detected on cells from lnly e minority of pstients. Tha objectives ox ghns proposed research are: (1) to induce qifferemtlation in fresrly psjlatsd human acute nonlymphoid leukemid cells (ANLL) by incunation with

surface receptors during normal human myeloid differentiation. markers, being used examine leukemic myeloid from a minority of The objectives of proposed research are: (1) to induce in freshly isolated human acute nonlymphoid leukemic cells (ANLL) by incubation with

surface receptors during norMal human myEloid DifFerEnTiatIon. THese membrane maRKers, Now being used to examine lEukemIc MYeloID dIfferEntiatiON aRE DetEcTeD on CeLLs From oNly A minoriTy of patienTs. THe Objectives of THiS proposed rEseArch are: (1) to indUce DifferEnTiaTIon in FreShly iSolateD Human aCute nonlyMpHOid leuKEmic celLS (aNlL) by Incubation with

surface receptors duringnormal hum an my elo iddi ffer enti ation. These m e mbra ne markers, now beingusedto exam i ne  leuk emic my e lo i d  di ff er ent ia t io n are  de tectedon cells f rom  o nly a minori t yof patient s.The objectiv esof thi spro p osedres earch  are:( 1) toinduce di ff e rentia t ion inf r es hlyisolated human ac u te nonlymphoid le ukemic  c e ll s (AN LL)  by incuba ti on wi t h

surface_receptors during_normal human myeloid differentiation._These membrane_markers,_now being_used_to examine leukemic_myeloid differentiation are_detected on cells from_only a minority_of_patients. The objectives of this proposed research are: (1) to induce differentiation in freshly_isolated_human acute_nonlymphoid_leukemic_cells (ANLL) by incubation with


human hosts, and how host hormones may regulate critical fungal processing during infection. We have described the presence of steroid binding proteins (SBPs) in C. albicans and other pathogenic fungi. These SBPs exhibit high affinity for selected mammalian steroid hormones, particularly estrogens and corticosteroids. We have also demonstrated functional responses in the fungi

human hosts, and how host hormones may regulate critical fungal processing during contagion. We have trace the presence of steroid bind protein (SBPs) in C. albicans and other pathogenic fungi. These SBPs expose high affinity for selected mammalian steroid hormone hormone, particularly estrogens and corticosteroid. We have also demonstrated running responses in the fungi

hulan hosts, and how host hurmones may regolqte crmtical rungal pfocessing during infection. Wx hace dewcribed the presence ow steroid binding proueins (SBPs) in C. albicans and otgcr pachigenic fungi. Tmese SBPs efhibit high afxivicy for selected mammalian steroid howmones, lagticularly esttogenx and bovticosteroids. We have also demonatrated functional rrsponses in the fungi

human hosts, and how host hormones may fungal during infection. have described the (SBPs) C. albicans and pathogenic fungi. These exhibit high affinity for selected mammalian hormones, particularly estrogens and corticosteroids. We have also demonstrated functional responses in the

human hosts, and how host hormoNes may reguLate cRitIcaL fUngaL proCessing during iNFectIon. We have described the pResenCe OF steROiD bindIng protEInS (sbPs) In c. aLbiCaNS aNd othEr pAthogenIc fungi. TheSe SbPS exhibit high AFfInity for seLecTed mammalian SteRoid hoRmOneS, PartiCulArly eStrogeNS and coRticosterOiDS. We havE Also demONStRateD functional responSEs IN the fungi

human hosts, and how host  hormonesmay r egu lat ecrit ical  fungal proces s ingduring infection. We h ave d es c ribe d  t he pr esenceo fs t ero id  b ind in g  p rotei ns(SBPs)in C. albi can sand other pa t ho genic fung i.These SBPs e xhi bit hi gh  af f inity  fo r sel ectedm ammali an steroi dh ormone s , parti c u la rlyestrogens and cor t ic o steroids. We h ave al so de m o nst rat ed functio na l res p onses i n  t h e fun g i

human_hosts, and_how host hormones may_regulate critical_fungal_processing during_infection._We have described_the presence of_steroid binding proteins (SBPs)_in C. albicans_and_other pathogenic fungi. These SBPs exhibit high affinity for selected mammalian steroid hormones, particularly_estrogens_and corticosteroids._We_have_also demonstrated functional responses in_the fungi


doctoral-level trainees. Trainees in the MS program may be supported on the TPG for up to 24 months. Trainees in the PhD program may be supported for up to 5 years. Oklahoma colleges have relatively high American Indian enrollments reflecting the state's unique ethnic mix, presenting an excellent pool of well qualified

doctoral - level trainees. Trainees in the MS program may be back on the TPG for up to 24 calendar month. Trainees in the PhD program may be support for up to 5 years. Oklahoma colleges hold relatively high American Indian enrollments reflect the state's unique heathen mix, presenting an excellent pool of well restricted

doftoral-level trainees. Tralnees in the MS krigram kay be supportdd on the TPG for up to 24 monvhs. Rraintvs in the PhD program may be slpported dor np to 5 years. Oklegoma colleges mave xeoatively high Smerican Itdian enrollmettr xeflecting the state's unique ethnic iix, prexejting an exceljent [ool of well qualified

doctoral-level trainees. Trainees in the MS program supported the TPG up to 24 program be supported for to 5 years. colleges have relatively high American Indian reflecting the state's unique ethnic mix, presenting an excellent pool of well qualified

doctoral-level trainees. TraiNees in the Ms progRam May Be SuppOrteD on the TPG for up TO 24 monThs. Trainees in the PhD proGram mAy BE supPOrTed foR up to 5 yeARs. oKLahOmA cOllEgES hAve reLatIvely hiGh American indIaN enrollments REfLecting the StaTe's unique ethNic Mix, preSeNtiNG an exCelLent pOol of wELl qualIfied

doctoral-level trainees.Trainees i n the  MS  pr og rammaybe supported o n  the  TPG for up to 24 mont hs. T ra i nees in  thePhD pro g ra m may  b esup po r te d for  up  to 5 y ears. Okla hom acolleges hav e  r elativelyhig h American I ndi an enr ol lme n ts re fle cting  the s t ate'sunique et hn i c mix, present i n gan e xcellent pool ofw el l  qualified

doctoral-level_trainees. Trainees_in the MS program_may be_supported_on the_TPG_for up to_24 months. Trainees_in the PhD program_may be supported_for_up to 5 years. Oklahoma colleges have relatively high American Indian enrollments reflecting the_state's_unique ethnic_mix,_presenting_an excellent pool of well_qualified


fusion is stimulated 40- fold by expression of a cloned Klebsiella pneumonia nifA gene. Pilin expression has been found to be regulated by two gene products from pilA and pilB. The relationship between pilA and pilB regulation and the sigma factor binding site will be investigated utilizing gene fusions and site

fusion is stimulated 40- fold by expression of a clone Klebsiella pneumonia nifA gene. Pilin formula has been found to be regulated by two gene product from pilA and pilB. The kinship between pilA and pilB regulation and the sigma factor binding web site will be investigate utilizing gene fusion and site

fudion is stimulated 40- fold by expression of a clmned Kmebsiellx pneumonia nifA gene. Pilin xxprwssiob has been found to be regulatef by two gent products from pmmA and inlB. Tgc relctmonship between pilA and [ilB regulatiot xnb the sigma factor binding site will be invrshigated utilizyng bqne rlslons and site

fusion is stimulated 40- fold by expression cloned pneumonia nifA Pilin expression has by gene products from and pilB. The between pilA and pilB regulation and sigma factor binding site will be investigated utilizing gene fusions and site

fusion is stimulated 40- fold by eXpression oF a cloNed kleBsIellA pneUmonia nifA gene. pIlin Expression has been found To be rEgULateD By Two geNe produCTs FROm pIla aNd pIlb. thE relaTioNship beTween pilA aNd pIlb regulation aND tHe sigma facTor Binding site wIll Be inveStIgaTEd utiLizIng geNe fusiONs and sIte

fusion is stimulated 40-fold by ex press ion  of  a  clo nedKlebsiella pne u moni a nifA gene. Pilin exp ressi on hasb ee n fou nd to b e  r e g ula te dbytw o  g ene p rod ucts fr om pilA an d p il B. The relat i on ship betwe enpilA and pil B r egulat io n a n d the  si gma f actorb inding  site wil lb e inve s tigated u ti lizi ng gene fusions a n ds ite

fusion_is stimulated_40- fold by expression_of a_cloned_Klebsiella pneumonia_nifA_gene. Pilin expression_has been found_to be regulated by_two gene products_from_pilA and pilB. The relationship between pilA and pilB regulation and the sigma factor_binding_site will_be_investigated_utilizing gene fusions and site


is known about how iron levels are regulated in the eye which is isolated from the systemic circulation by blood ocular barriers (BOB). The proposal's hypothesis is that intraocular tissues have unique and independent systems for regulating iron uptake into and efflux from the eye across the BOBs. Their polarized location and iron-regulated quantity

is known about how iron levels are regulated in the eye which is sequester from the systemic circulation by rake ocular barriers (BOB). The proposal's hypothesis is that intraocular tissue have unique and autonomous systems for regulating iron uptake into and efflux from the center across the bobsled. Their polarized localization and iron - regulated quantity

is known about how iron leyels are regulatgd in thx eye wgich is ksolated from the systemic cmrcuoatiob by blood ocular barrkers (BOB). Nhe propowal's yypothesis is that intrakgular vissues have unlque and ingependent systamr yor regulating iron uptake into and qfflux grlm the eye acrjss uhe BOBa. Their polarized location and iroh-regulaued quantity

is known about how iron levels are the which is from the systemic (BOB). proposal's hypothesis is intraocular tissues have and independent systems for regulating iron into and efflux from the eye across the BOBs. Their polarized location and quantity

is known about how iron levels Are regulatEd in tHe eYe wHiCh is IsolAted from the sysTEmic Circulation by blood oculAr barRiERs (BOb). thE propOsal's hyPOtHESis Is ThAt iNtRAoCular TisSues havE unique and IndEpEndent systemS FoR regulatinG irOn uptake into And Efflux FrOm tHE eye aCroSs the bOBs. ThEIr polaRized locaTiON and irON-regulaTED qUantIty

is known about how iron l evels areregul ate d i ntheeyewhich is isola t ed f rom the systemic circu latio nb y bl o od  ocul ar barr i er s (BO B) .The  p r op osal' s h ypothes is is that  in tr aocular tiss u es  have uniq ueand independ ent  syste ms  fo r  regu lat ing i ron up t ake in to and ef fl u x from the eye a cr ossthe BOBs. Their p o la r ized locationand ir on - re g u lat edquantity

is_known about_how iron levels are_regulated in_the_eye which_is_isolated from the_systemic circulation by_blood ocular barriers (BOB)._The proposal's hypothesis_is_that intraocular tissues have unique and independent systems for regulating iron uptake into and_efflux_from the_eye_across_the BOBs. Their polarized location_and iron-regulated quantity


Leica SP8 Laser Scanning Confocal Microscope or a PerkinElmer UltraView Spinning Disk Confocal Microscope, with some usage of our Total Internal Reflection Fluorescence (TIRF) microscope. Most of the users view immunofluorescent staining on fixed samples with the Leica LSCM

Leica SP8 Laser Scanning Confocal Microscope or a PerkinElmer UltraView Spinning Disk Confocal Microscope, with some usage of our Total Internal Reflection Fluorescence (TIRF) microscope. Most of the users view immunofluorescent staining on specify sample with the Leica LSCM

Lelca SP8 Laser Scanning Cokfocal Microscopg ir a PxrkinEljer UltrxView Spinning Disk Confocal Mucrosxope, with some usage ow our Totwl Interbal Ceflection Fluorxacence (BNRF) mjgroscm'e. Most of the osers view ikmunofluorescett scaining on fixed samples with the Leyca LSCK

Leica SP8 Laser Scanning Confocal Microscope or UltraView Disk Confocal with some usage Fluorescence microscope. Most of users view immunofluorescent on fixed samples with the Leica

Leica SP8 Laser Scanning ConfoCal MicroscOpe or A PeRkiNELmer ultrAView Spinning DISk CoNfocal Microscope, with soMe usaGe OF our tOtAl IntErnal ReFLeCTIon flUoResCeNCe (tIRF) mIcrOscope. MOst of the usErs ViEw immunofluoREsCent stainiNg oN fixed sampleS wiTh the LEiCa LscM

Leica SP8 Laser ScanningConfocal M icros cop e o ra Pe rkin Elmer UltraVie w  Spi nning Disk Confocal Mi crosc op e , wi t hsomeusage o f  o u r  To ta lInt er n al  Refl ect ion Flu orescence(TI RF ) microscope .  M ost of the  us ers view imm uno fluore sc ent stain ing  on f ixed s a mpleswith theLe i ca LSC M

Leica_SP8 Laser_Scanning Confocal Microscope or_a PerkinElmer_UltraView_Spinning Disk_Confocal_Microscope, with some_usage of our_Total Internal Reflection Fluorescence_(TIRF) microscope. Most_of_the users view immunofluorescent staining on fixed samples with the Leica LSCM


within the area as an educational mechanism. To undertake a retrospective study of the critical management variables of metastatic breast cancer. To study acute pulmonary distress associated with antiestrogen therapy in metastatic breast cancer patients. The undersigned agrees to accept responsibility for the scientific and technical conduct of the project and for provision of required progress reports if

within the area as an educational mechanism. To undertake a retrospective study of the critical management variables of metastatic breast cancer. To analyze acuate pulmonary distress associated with antiestrogen therapy in metastatic breast cancer patient. The undersigned agree to accept responsibility for the scientific and technical demeanor of the undertaking and for provision of required progress reports if

wihhin the area as an educxtional mechanism. To uidertaks a retruspective study of the critiral nanagtient variables of oetastatib breast xanctr. To study acute pulmonavv disfvess cswociated with sntiestrogan therapy in kegadtatic breast cancer patients. The ugdersigmef agrees to acsept wespknsibility for the scientific and fechnicel conduct of tne project and for provisiln ov required progresd reports id

within the area as an educational mechanism. a study of critical management variables study pulmonary distress associated antiestrogen therapy in breast cancer patients. The undersigned agrees accept responsibility for the scientific and technical conduct of the project and for of required progress reports if

within the area as an educatioNal mechaniSm. To uNdeRtaKe A retRospEctive study of tHE criTical management variablEs of mEtAStatIC bReast Cancer. TO StUDY acUtE pUlmOnARy DistrEss AssociaTed with antIesTrOgen therapy iN MeTastatic brEasT cancer patieNts. the undErSigNEd agrEes To accEpt resPOnsibiLity for thE sCIentifIC and tecHNIcAl coNduct of the project ANd FOr provision of rEquireD pROgRESs rEpoRts if

within the area as an edu cational m echan ism . T ounde rtak e a retrospect i ve s tudy of the critical m anage me n t va r ia blesof meta s ta t i c b re as t c an c er . Tostu dy acut e pulmonar y d is tress associ a te d with ant ies trogen thera pyin met as tat i c bre ast  canc er pat i ents.The under si g ned ag r ees toa c ce pt r esponsibility for th e  scientific an d tech ni c al c ond uct  of the pr oj ect a n d for p r ov i s i ono f required pr ogress repo r tsif

within_the area_as an educational mechanism._To undertake_a_retrospective study_of_the critical management_variables of metastatic_breast cancer. To study_acute pulmonary distress_associated_with antiestrogen therapy in metastatic breast cancer patients. The undersigned agrees to accept responsibility_for_the scientific_and_technical_conduct of the project and_for provision of required progress_reports if


interventions show promise as a behavioral technique to help staff and family caregivers improve the quality of life for people with dementia. Market potential exists for an engaging group activity for persons with dementia that provides appropriate levels of cognitive and physical involvement, accesses long-term memories, focuses attention and concentration, provides a high probability of success for

interventions show promise as a behavioral technique to help staff and syndicate health professional improve the quality of life for multitude with dementia. Market potential exist for an engaging group activeness for persons with dementia that supply appropriate levels of cognitive and physical participation, accesses long - terminus memory, focuses care and concentration, provide a eminent probability of success for

inherventions show promise as a behavioral technmque to help stxff and family caregivers im'rovw the quality of life for pdople witj dementua. Merket potential xsists for an skgagiug group activiti for personv with dementid ghct provides appropriate levels of codnitive ajd physical indolvtmegt, adbewses long-term memories, focusea attenuion and concentrayion, provides a high probahilihy of success for

interventions show promise as a behavioral technique staff family caregivers the quality of Market exists for an group activity for with dementia that provides appropriate levels cognitive and physical involvement, accesses long-term memories, focuses attention and concentration, provides a probability of success for

interventions show promise aS a behaviorAl tecHniQue To Help StafF and family careGIverS improve the quality of liFe for PeOPle wITh DemenTia. MarkET pOTEntIaL eXisTs FOr An engAgiNg group Activity foR peRsOns with demenTIa That providEs aPpropriate leVelS of cogNiTivE And phYsiCal inVolvemENt, acceSses long-tErM MemoriES, focuseS ATtEntiOn and concentratioN, PrOVides a high probAbilitY oF SuCCEss For

interventions show promis e as a beh avior altec hn ique  tohelp staff and fami ly caregivers improvethe q ua l ityo flifefor peo p le w ith  d em ent ia .  M arket  po tential  exists fo r a nengaging gro u pactivity f orpersons with  de mentia  t hat provi des  appr opriat e  level s of cogn it i ve and physica l in volv ement, accesses l o ng - term memories,  focus es at t e nti onand concen tr ation ,  provid e sa h igh probability o f success f o r

interventions_show promise_as a behavioral technique_to help_staff_and family_caregivers_improve the quality_of life for_people with dementia. Market_potential exists for_an_engaging group activity for persons with dementia that provides appropriate levels of cognitive and_physical_involvement, accesses_long-term_memories,_focuses attention and concentration, provides_a high probability of success_for


have been chemically synthesized for use in biological studies. The synthesis of (3H) indicine N-oxide proceeded from incidine N-oxide through a process involving reduction to indicine, ester cleavage, oxidation of the exocyclic carbon on the pyrrolizidine moiety, reduction with lithium aluminum tritide,

have been chemically synthesized for use in biological studies. The synthesis of (3H) indicine N - oxide go from incidine N - oxide through a procedure involving reduction to indicine, ester cleavage, oxidation of the exocyclic carbon on the pyrrolizidine moiety, decrease with lithium aluminum tritide,

hage been chemically synthtsized for use in biologmcal sthdies. Thd synthesis of (3H) indicine N-oeide proctvded from incidine N-obide throlgh a proxess unvolving csductiok to jkdiciue, ester cleavagg, oxidation mf the exocyclhc ccrbon on the pyrrolizidine moiety, requction wlth lithium alominuk trifpdt,

have been chemically synthesized for use in The of (3H) N-oxide proceeded from involving to indicine, ester oxidation of the carbon on the pyrrolizidine moiety, reduction lithium aluminum tritide,

have been chemically synthesIzed for use In bioLogIcaL sTudiEs. ThE synthesis of (3H) iNDiciNe N-oxide proceeded from iNcidiNe n-OxidE ThRough A procesS InVOLviNg ReDucTiON tO indiCinE, ester cLeavage, oxiDatIoN of the exocycLIc Carbon on thE pyRrolizidine mOieTy, reduCtIon WIth liThiUm aluMinum tRItide,

have been chemically synt hesized fo r use  in  bi ol ogic al s tudies. The sy n thes is of (3H) indicine N- oxide  p r ocee d ed  from  incidi n eN - oxi de  t hro ug h  a  proc ess  involv ing reduct ion  t o indicine,e st er cleavag e,oxidation of  th e exoc yc lic carbo n o n the  pyrro l izidin e moiety,  r e ductio n  with l i t hi um a luminum tritide,

have_been chemically_synthesized for use in_biological studies._The_synthesis of_(3H)_indicine N-oxide proceeded_from incidine N-oxide_through a process involving_reduction to indicine,_ester_cleavage, oxidation of the exocyclic carbon on the pyrrolizidine moiety, reduction with lithium aluminum_tritide,


-expression patterns that characterize retinal go and retina stop responses, and scleral mRNA and protein-expression patterns that characterize the sclera go, sclera stop and sclera ignores responses in our well-characterized animal model, tree shrew. These animals are closely related to primates and, like humans,

-expression patterns that characterize retinal go and retina stop answer, and scleral mRNA and protein - formula patterns that characterize the sclera go, sclera stop consonant and sclera ignores responses in our well - characterize animal mannequin, tree shrew. These animals are close relate to primates and, like homo,

-expgession patterns that chxracterize retiuql go end retjna stop responses, and scleral mRNA end proteun-expression patterns ghat charwcterize the wclera go, sclera sbjp ahf scnxra ignores reskonses in ous well-charactesixeb animal model, tree shrew. These animwls are cposely related to kriiatea and, like humans,

-expression patterns that characterize retinal go and responses, scleral mRNA protein-expression patterns that stop sclera ignores responses our well-characterized animal tree shrew. These animals are closely to primates and, like humans,

-expression patterns that chaRacterize rEtinaL go And ReTina Stop Responses, and scLEral MRNA and protein-expressiOn patTeRNs thAT cHaracTerize tHE sCLEra Go, ScLerA sTOp And scLerA ignoreS responses In oUr Well-characteRIzEd animal moDel, Tree shrew. TheSe aNimals ArE clOSely rElaTed to PrimatES and, liKe humans,

-expression patterns thatcharacteri ze re tin algo  and  ret ina stop respo n ses,  and scleral mRNA andprote in - expr e ss ion p atterns th a t  ch ar ac ter iz e  t he sc ler a go, s clera stop  an dsclera ignor e sresponsesinour well-cha rac terize dani m al mo del , tre e shre w . Thes e animals  a r e clos e ly rela t e dto p rimates and, like hu m ans,

-expression patterns_that characterize_retinal go and retina_stop responses,_and_scleral mRNA_and_protein-expression patterns that_characterize the sclera_go, sclera stop and_sclera ignores responses_in_our well-characterized animal model, tree shrew. These animals are closely related to primates and,_like_humans,


constants of a hydrogel sample. The shear modulus, m, the bulk modulus, k, and the hydraulic permeability, 1/f, can be determined from a single stress relaxation experiment. The material constants are treated as free parameters whose values are optimally estimated X)y minimizing the variance between predicted and empirical force relaxation wave

constants of a hydrogel sample. The shear modulus, m, the bulk modulus, k, and the hydraulic permeability, 1 / f, can be determined from a single tension liberalization experiment. The material constants are treat as detached parameters whose values are optimally calculate X)y understate the variance between predicted and empirical force liberalization wave

cojstants of a hydrogel saople. The shear modulus, m, the bulk moaulus, k, and the hydraulic pecmeavilitt, 1/f, can be determined wrom a sijgle strwss celaxation expermjent. Thc matsvial eoistants are trested as frae parameters fhusz values are optimally estimated X)y iinimizonh the variance beteqen lgebicted and empirical force relasation xave

constants of a hydrogel sample. The shear the modulus, k, the hydraulic permeability, a stress relaxation experiment. material constants are as free parameters whose values are estimated X)y minimizing the variance between predicted and empirical force relaxation wave

constants of a hydrogel samplE. The shear mOduluS, m, tHe bUlK modUlus, K, and the hydraulIC perMeability, 1/f, can be determiNed frOm A SingLE sTress RelaxatIOn EXPerImEnT. ThE mATeRial cOnsTants arE treated as FreE pArameters whoSE vAlues are opTimAlly estimateD X)y MinimiZiNg tHE variAncE betwEen preDIcted aNd empiricAl FOrce reLAxation WAVe

constants of a hydrogel s ample. The  shea r m odu lu s, m , th e bulk modulus ,  k,and the hydraulic perm eabil it y , 1/ f ,can b e deter m in e d  fr om  a  si ng l estres s r elaxati on experim ent .The material co nstants ar e t reated as fr eeparame te rsw hoseval ues a re opt i mallyestimated  X ) y mini m izing t h e  v aria nce between predi c te d  and empirical  force  r e la x a tio n w ave

constants_of a_hydrogel sample. The shear_modulus, m,_the_bulk modulus,_k,_and the hydraulic_permeability, 1/f, can_be determined from a_single stress relaxation_experiment._The material constants are treated as free parameters whose values are optimally estimated X)y_minimizing_the variance_between_predicted_and empirical force relaxation wave


restricted deficiency in RXRalpha; 3) To determine the role of RXRalpha pathways within endothelial/endocardial cushion cell lineages in cardiovascular morphogenesis by creating mice which harbor an endothelial cell lineage restricted deficiency in RXRalpha; 4) To determine the role of all retinoid receptor dependent pathways in specific cardiovascular cell types

restricted deficiency in RXRalpha; 3) To determine the role of RXRalpha nerve pathway within endothelial / endocardial shock absorber cell lineages in cardiovascular morphogenesis by create shiner which harbor an endothelial cell lineage qualify insufficiency in RXRalpha; 4) To determine the function of all retinoid receptor dependent pathway in specific cardiovascular cell types

redtricted deficiency in RWRalpha; 3) To detetmune thx role kf RXRaloha pathways within endothelmal/ebdocaedial cushion cell lindages in bardiovasxulac morphogenesis ug creatlug midc whieh harbor an endpthelial call lineage revtfieted deficiency in RXRalpha; 4) To detewmine tne role of all rgtinopd recsitir dependent pathways in specjfic cagdiovascular cell types

restricted deficiency in RXRalpha; 3) To determine of pathways within cushion cell lineages mice harbor an endothelial lineage restricted deficiency RXRalpha; 4) To determine the role all retinoid receptor dependent pathways in specific cardiovascular cell types

restricted deficiency in RXRAlpha; 3) To detErminE thE roLe Of RXralpHa pathways withIN endOthelial/endocardial cusHion cElL LineAGeS in caRdiovasCUlAR MorPhOgEneSiS By CreatIng Mice whiCh harbor an EndOtHelial cell liNEaGe restrictEd dEficiency in RxRaLpha; 4) To DeTerMIne thE roLe of aLl retiNOid recEptor depeNdENt pathWAys in spECIfIc caRdiovascular cell tYPeS

restricted deficiency inRXRalpha;3) To  de ter mi ne t he r ole of RXRalph a  pat hways within endotheli al/en do c ardi a lcushi on cell li n e age sin  ca rd i ov ascul armorphog enesis bycre at ing mice whi c hharbor anend othelial cel l l ineage  r est r icted  de ficie ncy in RXRalp ha; 4) To  d e termin e  the ro l e  o f al l retinoid recept o rd ependent pathw ays in  s p ec i f iccar diovascula rcellt ypes

restricted_deficiency in_RXRalpha; 3) To determine_the role_of_RXRalpha pathways_within_endothelial/endocardial cushion cell_lineages in cardiovascular_morphogenesis by creating mice_which harbor an_endothelial_cell lineage restricted deficiency in RXRalpha; 4) To determine the role of all retinoid_receptor_dependent pathways_in_specific_cardiovascular cell types


neural pathways which mediate the conversion of vocalization into endocrine response. In other words, we intend to demonstrate that an increased luteinizing hormone-releasing hormone (LHRH) release can be traced to neuronal activity in the vocal control nuclei associated with the performance of female nest calls. The proposed studies will add useful

neural pathways which mediate the conversion of vocalization into endocrine reception. In early words, we intend to attest that an increase luteinizing hormone - releasing hormone (LHRH) release can be hound to neural activity in the vocal control nuclei associated with the operation of female nest calls. The proposed studies will add utilitarian

nekral pathways which medixte the conversnin of tocalizztion ingo endocrine response. In othxr wirds, qe intend to demonstrage that aj increawed oyteinizing hormone-vzleasjkg hoxmine (LHRH) releaxe can be draced to neurmnxl activity in the vocal control nuclqi assoviwted with the kerfogmwnce of female nest calls. The proposed studiev will add usrful

neural pathways which mediate the conversion of endocrine In other we intend to hormone-releasing (LHRH) release can traced to neuronal in the vocal control nuclei associated the performance of female nest calls. The proposed studies will add useful

neural pathways which mediatE the converSion oF voCalIzAtioN intO endocrine respONse. IN other words, we intend to dEmonsTrATe thAT aN incrEased luTEiNIZinG hOrMonE-rELeAsing HorMone (LHRh) release caN be TrAced to neuronAL aCtivity in tHe vOcal control nUclEi assoCiAteD With tHe pErforMance oF Female Nest calls. thE ProposED studieS WIlL add Useful

neural pathways which med iate the c onver sio n o fvoca liza tion into endo c rine  response. In other wo rds,we inte n dto de monstra t et h atan  i ncr ea s ed  lute ini zing ho rmone-rele asi ng  hormone (LH R H)  release c anbe traced to  ne uronal  a cti v ity i n t he vo cal co n trol n uclei ass oc i ated w i th thep e rf orma nce of female nes t  c a lls. The propo sed st ud i es w ill  ad d useful

neural_pathways which_mediate the conversion of_vocalization into_endocrine_response. In_other_words, we intend_to demonstrate that_an increased luteinizing hormone-releasing_hormone (LHRH) release_can_be traced to neuronal activity in the vocal control nuclei associated with the performance_of_female nest_calls._The_proposed studies will add useful


and using this this model enables us to identify a DT:preF:adjuvant formulation that overcomes the risks of VED. In Phase I of this proposal, we will test four DT-preF:adjuvant formulations and identify the one that elicits the most protective responses that does not cause VED in

and using this this model enables us to identify a DT: preF: accessory conceptualization that get the best the risks of VED. In Phase I of this marriage proposal, we will test four DT - preF: accessory formulations and identify the one that arouse the most protective responses that does not induce VED in

anf using this this model tnables us to ideurify a DT:preR:adjuvang formulation that overcomes tye riwks of VED. In Phase I uf this pgoposal, ww wioo test fouc DT-preF:adjuvahb forknlations and idgntify the ote that elicitv ghz most protective responses that doef not csude VED in

and using this this model enables us a formulation that the risks of this we will test DT-preF:adjuvant formulations and the one that elicits the most responses that does not cause VED in

and using this this model enabLes us to ideNtify A DT:Pref:aDjuvAnt fOrmulation that OVercOmes the risks of VED. In PhaSe I of ThIS proPOsAl, we wIll test FOuR dt-prEF:AdJuvAnT FoRmulaTioNs and idEntify the oNe tHaT elicits the mOSt Protective ResPonses that doEs nOt causE VeD iN

and using this this model  enables u s toide nti fy  a D T:pr eF:adjuvant fo r mula tion that overcomes th e ris ks of V E D.  In P hase Io ft h ispr op osa l, we  will  te st four  DT-preF:a dju va nt formulati o ns  and ident ify  the one tha t e licits  t hem ost p rot ectiv e resp o nses t hat doesno t  cause VED in

and_using this_this model enables us_to identify_a_DT:preF:adjuvant formulation_that_overcomes the risks_of VED. In_Phase I of this_proposal, we will_test_four DT-preF:adjuvant formulations and identify the one that elicits the most protective responses that_does_not cause_VED_in


AA), myelodysplastic syndromes (MDS), and autoimmune disease, as well as genetic diseases, in conjunction with these projects, in vitro studies of cellular interactions will define how diseased cells in MDS or AA may be capable of suppressing normal hematopoiesis, possibly leading to alternative forms of therapy for these diseases

AA), myelodysplastic syndromes (MDS), and autoimmune disease, as well as genetic diseases, in junction with these undertaking, in vitro studies of cellular interactions will define how diseased cell in MDS or AA may be capable of suppressing normal hematopoiesis, possibly precede to alternate forms of therapy for these diseases

AA), lyelodysplastic syndromer (MDS), and autoimmune dmsease, zs well xs genetic diseases, in conjuictiin wiuk these projects, in xitro stufies of xellnlar interactions will dcyine glw dnsxased cells in KDS or AA kay be capable ow duppressing normal hematopoiesis, pofsibly kewding to altergatine forjs of therapy for these diseases

AA), myelodysplastic syndromes (MDS), and autoimmune disease, as diseases, in with these projects, interactions define how diseased in MDS or may be capable of suppressing normal possibly leading to alternative forms of therapy for these diseases

AA), myelodysplastic syndromeS (MDS), and autOimmuNe dIseAsE, as wEll aS genetic diseasES, in cOnjunction with these proJects, In VItro STuDies oF cellulAR iNTEraCtIoNs wIlL DeFine hOw dIseased Cells in MDS Or Aa mAy be capable oF SuPpressing nOrmAl hematopoieSis, PossibLy LeaDIng to AltErnatIve forMS of theRapy for thEsE DiseasES

AA), myelodysplastic syndr omes (MDS) , and  au toi mm unedise ase, as well a s  gen etic diseases, in conj uncti on with th ese p rojects ,  i n vit ro  s tud ie s  o f cel lul ar inte ractions w ill  d efine how di s ea sed cellsinMDS or AA ma y b e capa bl e o f  supp res singnormal hemato poiesis,po s siblyl eadingt o  a lter native forms of t h er a py for these d isease s

AA), myelodysplastic_syndromes (MDS),_and autoimmune disease, as_well as_genetic_diseases, in_conjunction_with these projects,_in vitro studies_of cellular interactions will_define how diseased_cells_in MDS or AA may be capable of suppressing normal hematopoiesis, possibly leading to_alternative_forms of_therapy_for_these diseases


of cell type- specific circuits tools and their function in the NHP brain. Temporal lobe epilepsy (TLE), the most common type of epilepsy, is characterized by destruction of specific hippocampal neurons. Recent evidence indicates that this neuronal damage is the result of excessive stimulation by excitotoxic amino acids (e.g., glut

of cell type- specific circuits tools and their function in the NHP mind. worldly lobe epilepsy (TLE), the most common character of epilepsy, is characterized by end of specific hippocampal neurons. Recent evidence argue that this neural damage is the result of excessive stimulation by excitotoxic amino acids (e.g., flood

of cell type- specific circmits tools and tkwir fuiction jn the NFP brain. Temporal lobe epile'sy (RLE), tye most common type of epilepsy, is charqctecized by destrucvjon of specifjg hip'orampal neurons. Tecent evidetce indicates dhxt this neuronal damage is the result of excrsdive stimulatijn bj qxcifotoxic amino acids (e.g., glut

of cell type- specific circuits tools and in NHP brain. lobe epilepsy (TLE), epilepsy, characterized by destruction specific hippocampal neurons. evidence indicates that this neuronal damage the result of excessive stimulation by excitotoxic amino acids (e.g., glut

of cell type- specific circuitS tools and tHeir fUncTioN iN the nHP bRain. Temporal loBE epiLepsy (TLE), the most common tYpe of EpILepsY, Is CharaCterizeD By DEStrUcTiOn oF sPEcIfic hIppOcampal Neurons. RecEnt EvIdence indicaTEs That this neUroNal damage is tHe rEsult oF eXceSSive sTimUlatiOn by exCItotoxIc amino acIdS (E.g., glut

of cell type- specific ci rcuits too ls an d t hei rfunc tion  in the NHP br a in.Temporal lobe epilepsy  (TLE ), them os t com mon typ e  o f epi le ps y,is ch aract eri zed bydestructio n o fspecific hip p oc ampal neur ons . Recent evi den ce ind ic ate s  that  th is ne uronal damage  is the r es u lt ofe xcessiv e st imul ation by excitoto x ic amino acids (e .g., g lu t

of_cell type-_specific circuits tools and_their function_in_the NHP_brain._Temporal lobe epilepsy_(TLE), the most_common type of epilepsy,_is characterized by_destruction_of specific hippocampal neurons. Recent evidence indicates that this neuronal damage is the result_of_excessive stimulation_by_excitotoxic_amino acids (e.g., glut


cancer research. The ultimate goal is to increase the number of health professionals, especially those from under-represented minorities, that will pursue clinical and/or research careers in cancer. The current grant award, Nutrition-Cancer Education Program (R25 CA47877) began September 1, 1988 and will end August 31,

cancer research. The ultimate goal is to increase the number of health professionals, particularly those from under - stage minorities, that will pursue clinical and/or research career in cancer. The current grant award, Nutrition - Cancer Education Program (R25 CA47877) begin September 1, 1988 and will end August 31,

cajcer research. The ultimaue goal is to incteqse thx numbed of heauth professionals, especially tyose drom under-represented oinoritied, that wull kursue clinical ais/or resccrch dwreexs in cancer. The current gsant award, Nutsigiln-Cancer Education Program (R25 CA47877) begwn Septrmher 1, 1988 and will end Wuguan 31,

cancer research. The ultimate goal is to number health professionals, those from under-represented and/or careers in cancer. current grant award, Education Program (R25 CA47877) began September 1988 and will end August 31,

cancer research. The ultimate Goal is to inCreasE thE nuMbEr of HealTh professionalS, EspeCially those from under-rePreseNtED minORiTies, tHat will PUrSUE clInIcAl aNd/OR rEsearCh cAreers iN cancer. The CurReNt grant award, nUtRition-CancEr EDucation ProgRam (r25 CA47877) begAn sepTEmber 1, 1988 And Will eNd AuguST 31,

cancer research. The ulti mate goalis to  in cre as e th e nu mber of health prof essionals, especiallythose  f r om u n de r-rep resente d  m i n ori ti es , t ha t  w ill p urs ue clin ical and/o r r es earch career s  i n cancer.The  current gra ntaward,  N utr i tion- Can cer E ducati o n Prog ram (R25CA 4 7877)b egan Se p t em ber1, 1988 and wille nd August 31,

cancer_research. The_ultimate goal is to_increase the_number_of health_professionals,_especially those from_under-represented minorities, that_will pursue clinical and/or_research careers in_cancer._The current grant award, Nutrition-Cancer Education Program (R25 CA47877) began September 1, 1988 and_will_end August_31,


ality using the Lung Aspiration Model. Specific Aim 3: To Validate the Efficacy of Cell-Permeable Antibodies for Post-Exposure Treatment in an Aerosolized Ricin Mouse Model. PUBLIC HEALTH RELEVANCE: Since there is currently no treatment for the ricin toxin, development of

ality using the Lung Aspiration Model. Specific Aim 3: To Validate the Efficacy of Cell - Permeable Antibodies for Post - Exposure Treatment in an Aerosolized Ricin Mouse Model. PUBLIC HEALTH RELEVANCE: Since there is currently no treatment for the ricin toxin, growth of

alihy using the Lung Aspirauion Model. Specifnx Aim 3: To Vamidate tfe Efficacy of Cell-Permeable Abtiboeies for Post-Exposure Greatment in an Awrosioized Ricii Mouse Model. LMBLIC IEALTH RELEVANCG: Since thera is currently nu creatment for the ricin toxin, develo[ment og

ality using the Lung Aspiration Model. Specific To the Efficacy Cell-Permeable Antibodies for Ricin Model. PUBLIC HEALTH Since there is no treatment for the ricin toxin, of

ality using the Lung AspiratiOn Model. SpeCific aim 3: to VAlIdatE the efficacy of Cell-pErmeAble Antibodies for Post-EXposuRe tReatMEnT in an aerosolIZeD rIciN MOuSe MOdEL. PuBLIC hEAlTH RELEvANCE: Since TheRe Is currently nO TrEatment for The Ricin toxin, deVelOpment Of

ality using the Lung Aspir ation Mode l. Sp eci fic  A im 3 : To  Validate theE ffic acy of Cell-PermeableAntib od i es f o rPost- Exposur e  T r e atm en tinan Ae rosol ize d Ricin  Mouse Mod el.  P UBLIC HEALTH RE LEVANCE: S inc e there is c urr entlyno  tr e atmen t f or th e rici n  toxin , develop me n t of

ality using_the Lung_Aspiration Model. Specific Aim_3: To_Validate_the Efficacy_of_Cell-Permeable Antibodies for_Post-Exposure Treatment in_an Aerosolized Ricin Mouse_Model. PUBLIC HEALTH_RELEVANCE:_Since there is currently no treatment for the ricin toxin, development of


Bruce Crise, Louis Henderson, and Karen Worthy. This patent describes a class of compounds with anti-HIV-1 activity, which are under investigation for use in antiretroviral therapy. [Corresponds to Rein Project 4 in the October 2011 site visit report of the HIV Drug Resistance Program] The investigators and

Bruce Crise, Louis Henderson, and Karen Worthy. This patent describes a class of compounds with anti - HIV-1 activity, which are under probe for habit in antiretroviral therapy. [ Corresponds to Rein Project 4 in the October 2011 site sojourn report of the HIV Drug Resistance Program ] The investigator and

Brkce Crise, Louis Hendersok, and Karen Wortkt. This patenf descrices a class of compounds witi anri-HIV-1 activity, which are unaer invesnigation dor nse in antiretrotjral thcxapy. [Dlrrev'onds to Rein Ptoject 4 in tve October 2011 side vnsit report of the HIV Drug Resistanse Progtal] The investigwtorx and

Bruce Crise, Louis Henderson, and Karen Worthy. describes class of with anti-HIV-1 activity, use antiretroviral therapy. [Corresponds Rein Project 4 the October 2011 site visit report the HIV Drug Resistance Program] The investigators and

Bruce Crise, Louis Henderson, aNd Karen WorThy. ThIs pAteNt DescRibeS a class of compoUNds wIth anti-HIV-1 activity, whicH are uNdER invEStIgatiOn for usE In ANTirEtRoVirAl THeRapy. [COrrEsponds To Rein ProjEct 4 In The October 2011 siTE vIsit report Of tHe HIV Drug ResIstAnce PrOgRam] tHe invEstIgatoRs and

Bruce Crise, Louis Hender son, and K arenWor thy .This  pat ent describesa  cla ss of compounds with a nti-H IV - 1 ac t iv ity,which a r eu n der  i nv est ig a ti on fo r u se in a ntiretrovi ral  t herapy. [Cor r es ponds to R ein  Project 4 i n t he Oct ob er2 011 s ite  visi t repo r t of t he HIV Dr ug Resist a nce Pro g r am ] Th e investigators a n d

Bruce_Crise, Louis_Henderson, and Karen Worthy._This patent_describes_a class_of_compounds with anti-HIV-1_activity, which are_under investigation for use_in antiretroviral therapy._[Corresponds_to Rein Project 4 in the October 2011 site visit report of the HIV_Drug_Resistance Program]_The_investigators_and


(apo) E in peripheral tissues. The proposal consists of 4 aims that are focused on activities of apoE that influence cellular cholesterol metabolism in adrenal cells and activities that are anti-atherogenic within the vascular wall. Experiments in Aim 1 will test the hypothesis that low levels of systemic apoE act within the vascular wall to

(apo) E in peripheral tissues. The proposal consists of 4 aim that are concenter on activities of apoE that influence cellular cholesterol metabolism in adrenal cell and activities that are anti - atherogenic within the vascular wall. experiment in Aim 1 will test the hypothesis that abject levels of systemic apoE act within the vascular wall to

(apl) E in peripheral tissuer. The proposal einsistv of 4 zims thag are focused on activities lf apoE that influence cellulxr cholesnerol metqbolmsm in adrenal cxmls and activjbies chet are anti-athetogenic withhn the vasculas dapl. Experiments in Aim 1 will test thq hypotnedis that low lgvels jf sgstemic apoE act within the vasculzr wall to

(apo) E in peripheral tissues. The proposal 4 that are on activities of metabolism adrenal cells and that are anti-atherogenic the vascular wall. Experiments in Aim will test the hypothesis that low levels of systemic apoE act within the wall to

(apo) E in peripheral tissues. ThE proposal cOnsisTs oF 4 aiMs That Are fOcused on activiTIes oF apoE that influence cellUlar cHoLEsteROl MetabOlism in ADrENAl cElLs And AcTIvIties ThaT are antI-atherogenIc wItHin the vasculAR wAll. ExperimEntS in Aim 1 will teSt tHe hypoThEsiS That lOw lEvels Of systEMic apoe act withiN tHE vascuLAr wall tO

(apo) E in peripheral tis sues. Thepropo sal  co ns ists  of4 aims that ar e  foc used on activities ofapoEth a t in f lu encecellula r  c h o les te ro l m et a bo lisminadrenal  cells and  ac ti vities thata re  anti-athe rog enic withinthe  vascu la r w a ll. E xpe rimen ts inA im 1 w ill testth e  hypot h esis th a t  l ow l evels of systemic ap o E act within t he vas cu l ar w all  to

(apo)_E in_peripheral tissues. The proposal_consists of_4_aims that_are_focused on activities_of apoE that_influence cellular cholesterol metabolism_in adrenal cells_and_activities that are anti-atherogenic within the vascular wall. Experiments in Aim 1 will test_the_hypothesis that_low_levels_of systemic apoE act within_the vascular wall to


programs. The objective of Phase II is to develop ProNutra, a research dietitian software tool for metabolic research studies, which was specified in Phase I. ProNutra contains several other innovative features, such as the ability to: automatically modify a study's diet schedule to meet the diet requirements of each of

programs. The objective of Phase II is to develop ProNutra, a research dietitian software cock for metabolic inquiry studies, which was specified in Phase I. ProNutra control respective other innovative features, such as the ability to: mechanically change a study's diet schedule to meet the diet requirements of each of

prlgrams. The objective of Khase II is to derwlop PcoNutra, a reseafch dietitian software tool hor netabilic research studies, dhich was specifiwd ii Phase I. ProNutcz contalus sebcral mvher innovative features, vuch as the abhlktv to: automatically modify a study's dyet schrdkle to meet thg dieu rqquidvmtnts of each of

programs. The objective of Phase II is ProNutra, research dietitian tool for metabolic in I. ProNutra contains other innovative features, as the ability to: automatically modify study's diet schedule to meet the diet requirements of each of

programs. The objective of PhaSe II is to deVelop pronutRa, A resEarcH dietitian softWAre tOol for metabolic researcH studIeS, WhicH WaS specIfied in pHaSE i. PrONUtRa cOnTAiNs sevEraL other iNnovative fEatUrEs, such as the aBIlIty to: automAtiCally modify a StuDy's dieT sCheDUle to MeeT the dIet reqUIremenTs of each oF

programs. The objective o f Phase II  is t o d eve lo p Pr oNut ra, a research diet itian software tool fo r met ab o licr es earch  studie s ,w h ich  w as  sp ec i fi ed in  Ph ase I.ProNutra c ont ai ns several o t he r innovati vefeatures, su chas the  a bil i ty to : a utoma ticall y  modif y a study 's diet s c hedulet o  m eetthe diet requirem e nt s  of each of

programs._The objective_of Phase II is_to develop_ProNutra,_a research_dietitian_software tool for_metabolic research studies,_which was specified in_Phase I. ProNutra_contains_several other innovative features, such as the ability to: automatically modify a study's diet_schedule_to meet_the_diet_requirements of each of


25RR022703); and our currently project (2014- 2019), A New Genomic Framework for Schools and Communities (Grant Code: 8 R25 GM129186-05). These projects focused on addressing the critical need for modern genomics curriculum for middle and high school students by connecting classroom instruction with the community to

25RR022703); and our currently project (2014- 2019), A New Genomic Framework for Schools and Communities (Grant Code: 8 R25 GM129186 - 05). These projects focused on address the critical motivation for modern genomics curriculum for middle and gamey school students by connecting classroom direction with the community to

25RR022703); wnd our currently projecu (2014- 2019), A New Genomic Framewmrk fod Schoolr and Communities (Grant Code: 8 E25 GM129186-05). Ukese projects focusea on addrvssing thw crmtical need for modern gcuomica curxirulum for middlg and high swhool students bh eonnecting classroom instruction witr the cpmlunity to

25RR022703); and our currently project (2014- 2019), Genomic for Schools Communities (Grant Code: focused addressing the critical for modern genomics for middle and high school students connecting classroom instruction with the community to

25RR022703); and our currently project (2014- 2019), A new Genomic frameWorK foR SChooLs anD Communities (GrANt CoDe: 8 R25 GM129186-05). These projects focuSed on AdDRessINg The crItical nEEd FOR moDeRn GenOmICs CurriCulUm for miDdle and higH scHoOl students by COnNecting claSsrOom instructiOn wIth the CoMmuNIty to

25RR022703); and our curre ntly proje ct (2 014 - 2 01 9),A Ne w Genomic Fram e work  for Schools and Commu nitie s( Gran t  C ode:8 R25 G M 12 9 1 86- 05 ).  Th es e  p rojec tsfocused  on addres sin gthe critical ne ed for mod ern  genomics cu rri culumfo r m i ddleand  high  schoo l  stude nts by co nn e ctingc lassroo m in stru ction with the co m mu n ity to

25RR022703); and_our currently_project (2014- 2019), A_New Genomic_Framework_for Schools_and_Communities (Grant Code:_8 R25 GM129186-05)._These projects focused on_addressing the critical_need_for modern genomics curriculum for middle and high school students by connecting classroom instruction_with_the community_to


; to determine the maximum tolerated does (MTD) of SPI-77 when given once every 2 weeks for 2 and 3 doses concurrent with radiotheraphy; to determine the pharmacokinetics of SPI-77; and to gather preliminary response information that may result from the treatment of SPI-77 when given concurrent with radi

; to determine the maximum tolerated does (MTD) of SPI-77 when give once every 2 week for 2 and 3 doses concurrent with radiotheraphy; to determine the pharmacokinetics of SPI-77; and to accumulate preliminary response information that may leave from the treatment of SPI-77 when given concurrent with radi

; to determine the maximum tulerated does (MJD) of SPM-77 when fiven onze every 2 weeks for 2 and 3 dodew conxurrent with radiotherxphy; to dvtermine rhe kharmacokinetics of SPI-77; akb to fwthex 'reliminary reskonse informdtion that may rdsblt from the treatment of SPI-77 when gyven comckrrent with raqi

; to determine the maximum tolerated does SPI-77 given once 2 weeks for with to determine the of SPI-77; and gather preliminary response information that may from the treatment of SPI-77 when given concurrent with radi

; to determine the maximum toleRated does (MtD) of SpI-77 wHen GiVen oNce eVery 2 weeks for 2 anD 3 DoseS concurrent with radiothEraphY; tO DeteRMiNe the PharmacOKiNETicS oF SpI-77; aNd TO gAther PreLiminarY response iNfoRmAtion that may REsUlt from the TreAtment of SPI-77 wHen Given cOnCurREnt wiTh rAdi

; to determine the maximum  tolerated  does  (M TD)  o f SP I-77  when given on c e ev ery 2 weeks for 2 and3 dos es conc u rr ent w ith rad i ot h e rap hy ;tode t er minethe  pharma cokinetics  of  S PI-77; and t o  g ather prel imi nary respons e i nforma ti ont hat m ayresul t from the tr eatment o fS PI-77w hen giv e n  c oncu rrent with radi

; to_determine the_maximum tolerated does (MTD)_of SPI-77_when_given once_every_2 weeks for_2 and 3_doses concurrent with radiotheraphy;_to determine the_pharmacokinetics_of SPI-77; and to gather preliminary response information that may result from the treatment_of_SPI-77 when_given_concurrent_with radi


as a paracrine mediator of progenitor recruitment and osteogenic differentiation in load- and injury-induced osteogenesis. We further hypothesize that SDF-1 treatment can accelerate bone repair in aged mice, a model of diminished regenerative capacity, possibly by enhancing cellular recruitment. To our hypotheses, we propose the following Specific Aims

as a paracrine mediator of progenitor recruitment and osteogenic differentiation in load- and injury - induced osteogenesis. We further speculate that SDF-1 discussion can accelerate bone haunt in aged mice, a model of diminished regenerative capacitance, possibly by enhancing cellular recruitment. To our hypotheses, we project the following Specific Aims

as a paracrine mediator of progenitor rectuutment and oateogeniz differentiation in load- anv inhury-ibduced osteogenesis. We further jypothesuze uhat SDF-1 treatmenv can acgzlerafc bonz cepair in aged kice, a modal of diminishad rzgenerative capacity, possibly by enhwncing veplular recruitient. Eo ohg mypotheses, we propose the followjng Spebific Aims

as a paracrine mediator of progenitor recruitment differentiation load- and osteogenesis. We further accelerate repair in aged a model of regenerative capacity, possibly by enhancing cellular To our hypotheses, we propose the following Specific Aims

as a paracrine mediator of proGenitor recRuitmEnt And OsTeogEnic DifferentiatioN In loAd- and injury-induced osteOgeneSiS. we fuRThEr hypOthesizE ThAT sDF-1 TrEaTmeNt CAn AccelEraTe bone rEpair in ageD miCe, A model of dimiNIsHed regenerAtiVe capacity, poSsiBly by eNhAncINg celLulAr recRuitmeNT. To our HypotheseS, wE ProposE The follOWInG SpeCific Aims

as a paracrine mediator o f progenit or re cru itm en t an d os teogenic diffe r enti ation in load- and inj ury-i nd u cedo st eogen esis. W e  f u r the rhy pot he s iz e tha t S DF-1 tr eatment ca n a cc elerate bone re pair in ag edmice, a mode l o f dimi ni she d  rege ner ative  capac i ty, po ssibly by  e n hancin g  cellul a r  r ecru itment. To our hy p ot h eses, we propo se the  f o ll o w ing  Sp ecific Aim s

as_a paracrine_mediator of progenitor recruitment_and osteogenic_differentiation_in load-_and_injury-induced osteogenesis. We_further hypothesize that_SDF-1 treatment can accelerate_bone repair in_aged_mice, a model of diminished regenerative capacity, possibly by enhancing cellular recruitment. To our_hypotheses,_we propose_the_following_Specific Aims


comparison of genotype to phenotypes. We have collected all of the DNA samples and relevant phenotypes, and are ready to proceed with high-throughput sequencing in order to calculate mutation rates of great relevance to biomedicine. In Aim 1 we focus on CNVs, which are readily detected at low coverage sequencing due to our selection of mice

comparison of genotype to phenotypes. We have collected all of the DNA samples and relevant phenotype, and are quick to continue with high - throughput sequencing in orderliness to calculate mutation rate of capital relevance to biomedicine. In Aim 1 we focus on CNVs, which are readily detected at broken coverage sequencing due to our selection of shiner

colparison of genotype to khenotypes. We havg xollecved all of the ANA samples and relevant pheiotypes, abd are ready to proceea with hihh-througyput wequencing in ordev to dwlcunete mutation rajes of great relevance to tiumzdicine. In Aim 1 we focus on CNVs, whish are tewdily detected at kjw cknevage sequencing due to our selecfion of mice

comparison of genotype to phenotypes. We have of DNA samples relevant phenotypes, and high-throughput in order to mutation rates of relevance to biomedicine. In Aim 1 focus on CNVs, which are readily detected at low coverage sequencing due to selection of mice

comparison of genotype to pheNotypes. We hAve coLleCteD aLl of The DnA samples and reLEvanT phenotypes, and are ready To proCeED witH HiGh-thrOughput SEqUENciNg In OrdEr TO cAlculAte MutatioN rates of grEat ReLevance to bioMEdIcine. In Aim 1 We fOcus on CNVs, whIch Are reaDiLy dETecteD at Low coVerage SEquencIng due to oUr SElectiON of mice

comparison of genotype to  phenotype s. We  ha veco llec tedall of the DNA samp les and relevant pheno types ,a nd a r eready  to pro c ee d wit hhi gh- th r ou ghput  se quencin g in order  to  c alculate mut a ti on rates o f g reat relevan ceto bio me dic i ne. I n A im 1we foc u s on C NVs, whic ha re rea d ily det e c te d at  low coverage seq u en c ing due to our  selec ti o no f  mi ce

comparison_of genotype_to phenotypes. We have_collected all_of_the DNA_samples_and relevant phenotypes,_and are ready_to proceed with high-throughput_sequencing in order_to_calculate mutation rates of great relevance to biomedicine. In Aim 1 we focus on_CNVs,_which are_readily_detected_at low coverage sequencing due_to our selection of mice


specific aim investigates whether S. Aureus growing on the skin of patients with an exacerbation of AD due to infection, produce toxins known to act as bacterial superantigens E.G. Staphylococcal enterotoxins (SES) and correlate these findings with the TCR VB gene usage of the t cell infiltr

specific aim investigates whether S. Aureus growing on the skin of patients with an aggravation of AD due to contagion, grow toxins know to dissemble as bacterial superantigens E.G. Staphylococcal enterotoxins (SES) and correlate these findings with the TCR VB gene custom of the triiodothyronine cell infiltr

spfcific aim investigates dhether S. Aureus growiig on tge skin uf patients with an exacerbavion of AE due to infection, proauce toxijs known to ect as bacterial superanbngens C.G. Stcpiylococcal entetotoxins (SES) and correlate tfede findings with the TCR VB gene uswge of yhf t cell infiljr

specific aim investigates whether S. Aureus growing skin patients with exacerbation of AD known act as bacterial E.G. Staphylococcal enterotoxins and correlate these findings with the VB gene usage of the t cell infiltr

specific aim investigates whEther S. AureUs groWinG on ThE skiN of pAtients with an eXAcerBation of AD due to infectiOn, proDuCE toxINs Known To act as BAcTERiaL sUpEraNtIGeNs E.G. STapHylococCal enterotOxiNs (sES) and correlATe These findiNgs With the TCR VB GenE usage Of The T Cell iNfiLtr

specific aim investigates  whether S . Aur eus  gr ow ingon t he skin of pat i ents  with an exacerbationof AD  d u e to in fecti on, pro d uc e tox in skno wn to  actasbacteri al superan tig en s E.G. Staph y lo coccal ent ero toxins (SES)  an d corr el ate these  fi nding s with the TC R VB gene  u s age of the t c e l linfi ltr

specific_aim investigates_whether S. Aureus growing_on the_skin_of patients_with_an exacerbation of_AD due to_infection, produce toxins known_to act as_bacterial_superantigens E.G. Staphylococcal enterotoxins (SES) and correlate these findings with the TCR VB gene_usage_of the_t_cell_infiltr


extracellular matrix (ECM) exerts on type II pneumocytes. The extracellular components of the lung have long been known to influence the physical/mechanical properties of the tissue and thereby to play an important role in the function of normal and diseased tissue. alterations in the content and/or organization of connective tissue elements are involved

extracellular matrix (ECM) exerts on type II pneumocytes. The extracellular components of the lung have long been known to influence the forcible / mechanical place of the tissue and thereby to play an important function in the routine of normal and diseased tissue. alterations in the content and/or arrangement of connective tissue elements are involved

exhracellular matrix (ECM) ewerts on type II pneumorytes. Tge extrazellular components of the lnng yave oong been known to infuuence thv physicao/mecianical propertixa of thc tisame anb vhereby to play an importdnt role in tha wuuction of normal and diseased tissue. alterayilns in the conjent sgd/or organization of connective tissue elemenus are involved

extracellular matrix (ECM) exerts on type II extracellular of the have long been properties the tissue and to play an role in the function of normal diseased tissue. alterations in the content and/or organization of connective tissue elements are

extracellular matrix (ECM) exeRts on type Ii pneuMocYteS. THe exTracEllular componeNTs of The lung have long been knoWn to iNfLUencE ThE physIcal/mecHAnICAl pRoPeRtiEs OF tHe tisSue And therEby to play aN imPoRtant role in tHE fUnction of nOrmAl and diseaseD tiSsue. alTeRatIOns in The ConteNt and/oR OrganiZation of cOnNEctive TIssue elEMEnTs arE involved

extracellular matrix (ECM ) exerts o n typ e I I p ne umoc ytes . The extracel l ular  components of the lun g hav el ongb ee n kno wn to i n fl u e nce  t he  ph ys i ca l/mec han ical pr operties o f t he  tissue andt he reby to pl ayan important  ro le inth e f u nctio n o f nor mal an d  disea sed tissu e. altera t ions in t he  con tent and/or organ i za t ion of connect ive ti ss u ee l eme nts  are invol ve d

extracellular_matrix (ECM)_exerts on type II_pneumocytes. The_extracellular_components of_the_lung have long_been known to_influence the physical/mechanical properties_of the tissue_and_thereby to play an important role in the function of normal and diseased tissue._alterations_in the_content_and/or_organization of connective tissue elements_are involved


cause of death for youth and the 10th leading cause of death for adults. As suicide risk screening becomes a national priority for medical settings, non-mental health clinicians require valid, psychometrically-sound screening instruments for assessing suicide risk. However, there are very few such mental health assessment instruments that have been validated empirically

cause of death for youth and the 10th lead lawsuit of death for adults. As suicide risk cover becomes a national priority for aesculapian context, non - mental health clinician command valid, psychometrically - sound cover instruments for assessing suicide risk. However, there are very few such genial health assessment instruments that have been validate empirically

cakse of death for youth akd the 10th leadiny cause of dezth for xdults. As suicide risk screeiing becones a national priorith for medpcal settungs, bon-mental isalth clinicizks rexnire valid, psycmometricallf-sound screenitg iustruments for assessing suicide rish. Howevrr, there are veri few fuch mental health assessment instrumehts thau have been validayed empirically

cause of death for youth and the cause death for As suicide risk for settings, non-mental health require valid, psychometrically-sound instruments for assessing suicide risk. However, are very few such mental health assessment instruments that have been validated empirically

cause of death for youth and thE 10th leading Cause Of dEatH fOr adUlts. as suicide risk sCReenIng becomes a national priOrity FoR MediCAl SettiNgs, non-mENtAL HeaLtH cLinIcIAnS requIre Valid, psYchometricAllY-sOund screeninG InStruments fOr aSsessing suicIde Risk. HoWeVer, THere aRe vEry feW such mENtal heAlth assesSmENt instRUments tHAT hAve bEen validated empirICaLLy

cause of death for youthand the 10 th le adi ngca useof d eath for adult s . As  suicide risk screenin g bec om e s an at ional  priori t yf o r m ed ic alse t ti ngs,non -mental  health cl ini ci ans requirev al id, psycho met rically-soun d s creeni ng  in s trume nts  forassess i ng sui cide risk .H owever ,  therea r every  few such mentalh ea l th assessmentinstru me n ts t hat  ha ve been va li dated empiric a ll y

cause_of death_for youth and the_10th leading_cause_of death_for_adults. As suicide_risk screening becomes_a national priority for_medical settings, non-mental_health_clinicians require valid, psychometrically-sound screening instruments for assessing suicide risk. However, there are very_few_such mental_health_assessment_instruments that have been validated_empirically