cells reconstitute a functional ventricular wall. A decrease in their number and/or function might be one of the causal factors in the altered performance of the old and senescent heart. Nothing is known about the origin of the CSCs. Data from animals and humans show a continuous trafficking of these cells between myocardium and extra-

cells reconstitute a functional ventricular wall. A decrease in their numeral and/or affair might be one of the causal factors in the altered performance of the honest-to-god and senescent heart. Nothing is know about the origin of the CSCs. Data from animal and world show a continuous trafficking of these cell between myocardium and extra-

cepls reconstitute a functlonal ventriculat qall. A decrezse in tfeir number and/or function mmght be obe of the causal factofs in the altered perhormance of the old and senescskt hecrv. Nothing is knpwn about dhe origin of dhd ESCs. Data from animals and humans shjw a comtlnuous traffiching jf tgvst cells between myocardium and exfra-

cells reconstitute a functional ventricular wall. A their and/or function be one of altered of the old senescent heart. Nothing known about the origin of the Data from animals and humans show a continuous trafficking of these cells between and extra-

cells reconstitute a functioNal ventricUlar wAll. a deCrEase In thEir number and/or FUnctIon might be one of the causAl facToRS in tHE aLtereD perforMAnCE Of tHe OlD anD sENeScent HeaRt. NothiNg is known aBouT tHe origin of thE cScs. Data from AniMals and humanS shOw a conTiNuoUS trafFicKing oF these CElls beTween myocArDIum and EXtra-

cells reconstitute a func tional ven tricu lar  wa ll . Adecr ease in theirn umbe r and/or function migh t beon e  oft he  caus al fact o rs i n t he  a lte re d  p erfor man ce of t he old and  se ne scent heart. No thing is k now n about theori gin of  t heC SCs.Dat a fro m anim a ls and  humans s ho w  a con t inuoust r af fick ing of these cell s  b e tween myocardi um and  e x tr a -

cells_reconstitute a_functional ventricular wall. A_decrease in_their_number and/or_function_might be one_of the causal_factors in the altered_performance of the_old_and senescent heart. Nothing is known about the origin of the CSCs. Data from_animals_and humans_show_a_continuous trafficking of these cells_between myocardium and extra-


. Lymphotoxin (LT), a product of antigen or mitogen activated T cells, is being studied as a model lymphokine. The long term goals are to study LT's cell of origin, biochemical nature, mechanism of action, relationship to other lymphokines, regulation and role in vivo. Murine lymphotoxin

. Lymphotoxin (LT), a product of antigen or mitogen activated T cells, is being study as a exemplar lymphokine. The long term goals are to learn LT's cell of origin, biochemical nature, mechanism of military action, relationship to other lymphokine, rule and role in vivo. Murine lymphotoxin

. Lylphotoxin (LT), a product on antigen or mitogen acvivated T cells, is being studied as a model ltmphojine. The long term goaus are to study LR's ctll of origin, biorgemical naturs, meckaiism of action, telationship to other lympvoyiues, regulation and role in vivo. Murige lympnohoxin

. Lymphotoxin (LT), a product of antigen activated cells, is studied as a goals to study LT's of origin, biochemical mechanism of action, relationship to other regulation and role in vivo. Murine lymphotoxin

. Lymphotoxin (LT), a product of anTigen or mitOgen aCtiVatEd t celLs, is Being studied as A ModeL lymphokine. The long term Goals ArE To stUDy lT's ceLl of oriGIn, BIOchEmIcAl nAtURe, MechaNisM of actiOn, relationShiP tO other lymphoKInEs, regulatiOn aNd role in vivo. murIne lymPhOtoXIn

. Lymphotoxin (LT), a prod uct of ant igenormit og en a ctiv ated T cells,i s be ing studied as a model  lymp ho k ine. Th e lon g termg oa l s  ar eto  st ud y  L T's c ell  of ori gin, bioch emi ca l nature, me c ha nism of ac tio n, relations hip  to ot he r l y mphok ine s, re gulati o n androle in v iv o . Muri n e lymph o t ox in

. Lymphotoxin_(LT), a_product of antigen or_mitogen activated_T_cells, is_being_studied as a_model lymphokine. The_long term goals are_to study LT's_cell_of origin, biochemical nature, mechanism of action, relationship to other lymphokines, regulation and role_in_vivo. Murine_lymphotoxin


and derivatization of novel DNA-binding peptides and detailed characterization of their DNA-binding and -condensing properties. A series of low molecular weight branched oligolysines and oligoarginines with a defined coupling site will be synthesized. We will determine the effect of number and positioning of positive charges on DNA condensation.

and derivatization of novel DNA - binding peptides and detailed word picture of their deoxyribonucleic acid - binding and -condensing properties. A serial of low molecular weight unit branched oligolysines and oligoarginines with a defined yoke web site will be synthesized. We will determine the effect of number and positioning of positivist charges on DNA condensing.

anf derivatization of noveu DNA-binding pektudes aid detajled chafacterization of their DNA-biidint and -condensing properties. A series of low nolerular weight braidhed ollyolysjkes aud oligoargininex with a dafined couplinc rice will be synthesized. We will deteriine thr fffect of numbgr anc posjniining of positive charges on SNA convensation.

and derivatization of novel DNA-binding peptides and of DNA-binding and properties. A series oligolysines oligoarginines with a coupling site will synthesized. We will determine the effect number and positioning of positive charges on DNA condensation.

and derivatization of novel DnA-binding pEptidEs aNd dEtAileD chaRacterization oF TheiR DNA-binding and -condensiNg proPeRTies. a SeRies oF low molECuLAR weIgHt BraNcHEd OligoLysInes and OligoarginIneS wIth a defined cOUpLing site wiLl bE synthesized. we wIll detErMinE The efFecT of nuMber anD PositiOning of poSiTIve chaRGes on DNa COnDensAtion.

and derivatization of nov el DNA-bin dingpep tid es  and  det ailed characte r izat ion of their DNA-bindi ng an d- cond e ns ing p roperti e s. A  se ri es  of  l o wmolec ula r weigh t branched  ol ig olysines and ol igoarginin eswith a defin edcoupli ng  si t e wil l b e syn thesiz e d. Wewill dete rm i ne the effecto f  n umbe r and positioning of positive charg es onDN A  c o n den sat ion.

and_derivatization of_novel DNA-binding peptides and_detailed characterization_of_their DNA-binding_and_-condensing properties. A_series of low_molecular weight branched oligolysines_and oligoarginines with_a_defined coupling site will be synthesized. We will determine the effect of number and_positioning_of positive_charges_on_DNA condensation.


orously the ultimate performance characteristics of the human visual system and to delimit the range of possible neural circuits that may serve as their substrate, and (2) to gain insight into the optical and neural processes subserving visual acuity, hyperacuity and stereoscopic localization that will aid in the differential diagnosis of patients with reduced acuity

orously the ultimate performance characteristics of the human visual system and to demarcate the compass of possible neural circuits that may suffice as their substrate, and (2) to gain insight into the ocular and nervous processes subserving visual acuity, hyperacuity and stereoscopic localization that will help in the differential diagnosis of patients with reduced acuteness

oroksly the ultimate perforoance characternwtics mf the human vksual system and to delimit vhe eange of possible neural cifcuits thwt may swrve qs their sngstrate, and (2) fl gann insight into jhe optical dnd neural prowerszs subserving visual acuity, hyperacuyty and shereoscopic losalieatyon fhat will aid in the differential siagnosps of patients wiyh reduced acuity

orously the ultimate performance characteristics of the system to delimit range of possible as substrate, and (2) gain insight into optical and neural processes subserving visual hyperacuity and stereoscopic localization that will aid in the differential diagnosis of patients reduced acuity

orously the ultimate performAnce characTerisTicS of ThE humAn viSual system and tO DeliMit the range of possible nEural CiRCuitS ThAt may Serve as THeIR SubStRaTe, aNd (2) TO gAin inSigHt into tHe optical aNd nEuRal processes SUbServing visUal Acuity, hyperaCuiTy and sTeReoSCopic LocAlizaTion thAT will aId in the diFfERentiaL DiagnosIS Of PatiEnts with reduced acUItY

orously the ultimate perfo rmance cha racte ris tic sof t he h uman visual sy s temand to delimit the ran ge of  p o ssib l eneura l circu i ts t hat  m ay  se rv e  a s the irsubstra te, and (2 ) t ogain insight in to the opt ica l and neural  pr ocesse ssub s ervin g v isual  acuit y , hype racuity a nd stereo s copic l o c al izat ion that will aid in the differenti al dia gn o si s ofpat ients with  r educe d  acuity

orously the_ultimate performance_characteristics of the human_visual system_and_to delimit_the_range of possible_neural circuits that_may serve as their_substrate, and (2)_to_gain insight into the optical and neural processes subserving visual acuity, hyperacuity and stereoscopic_localization_that will_aid_in_the differential diagnosis of patients_with reduced acuity


nursing homes and assisted living facilities as well as informal (family) caregivers providing care in the home, using DVD/Web DVD technology. AIMS: Translate current evidence based research on sleep disturbances among dementia patients into a comprehensive training module of all-inclusive strategies aimed at improving sleep for nursing home and assisted living

nursing homes and assisted living facilities equally well as cozy (class) caregivers providing concern in the home, using DVD / vane DVD technology. AIMS: understand current evidence free-base research on rest disturbances among dementia patients into a comprehensive training module of all - inclusive scheme aimed at improving sleep for nursing home and assisted life

nugsing homes and assisted living facilitnws as xell as informau (family) caregivers providinj caee in the home, using DVD/Web DVD techjology. AUMS: Uranslate current evidencc bassf revxarch on sleep cisturbancas among demendix 'atients into a comprehensive trainigg moduke of all-incluside suraeegiss aimed at improving sleep for nudsing hmme and assisyed living

nursing homes and assisted living facilities as informal caregivers providing in the home, Translate evidence based research sleep disturbances among patients into a comprehensive training module all-inclusive strategies aimed at improving sleep for nursing home and assisted living

nursing homes and assisted liVing faciliTies aS weLl aS iNforMal (fAmily) caregiverS ProvIding care in the home, usinG DVD/WEb dvD teCHnOlogy. aIMS: TraNSlATE cuRrEnT evIdENcE baseD reSearch oN sleep distUrbAnCes among demeNTiA patients iNto A comprehensiVe tRaininG mOduLE of alL-inClusiVe straTEgies aImed at impRoVIng sleEP for nurSINg Home And assisted living

nursing homes and assiste d living f acili tie s a swell  asinformal (fami l y) c aregivers providing ca re in  t h e ho m e,  usin g DVD/W e bD V D t ec hn olo gy .  A IMS:Tra nslatecurrent ev ide nc e based rese a rc h on sleep  di sturbances a mon g deme nt iap atien tsintoa comp r ehensi ve traini ng module of all- i n cl usiv e strategies aime d  a t  improving sle ep for  n u rs i n g h ome  and assis te d liv i ng

nursing_homes and_assisted living facilities as_well as_informal_(family) caregivers_providing_care in the_home, using DVD/Web_DVD technology. AIMS: Translate_current evidence based_research_on sleep disturbances among dementia patients into a comprehensive training module of all-inclusive strategies_aimed_at improving_sleep_for_nursing home and assisted living


recombination, may involve DSBs and thus, employ NHEJ. We have shown that defective NHEJ during attempted V(D)J recombination in a p53 tumor-suppressor deficient background reproducibly leads to murine pro-B cell lymphomas that harbor translocations between the IgH and c-myc

recombination, may involve DSBs and thus, employ NHEJ. We have shown that bad NHEJ during try V(D)J recombination in a p53 tumor - suppressor deficient background reproducibly lead to murine pro - B cellular telephone lymphomas that harbor translocations between the IgH and c - myc

refombination, may involve ASBs and thus, employ NIEJ. We gave shodn that defective NHEJ durinj atrempttb V(D)J recombination kn a p53 tulor-supprwssoc deficient backjdound rc'rodudlbly nxads to murine kro-B cell lykphomas that hdrcox translocations between the IgH and c-myc

recombination, may involve DSBs and thus, employ have that defective during attempted V(D)J deficient reproducibly leads to pro-B cell lymphomas harbor translocations between the IgH and

recombination, may involve DSbs and thus, eMploy nHEj. We HaVe shOwn tHat defective NHej durIng attempted V(D)J recombiNatioN iN A p53 tuMOr-SupprEssor deFIcIENt bAcKgRouNd REpRoducIblY leads tO murine pro-b ceLl Lymphomas thaT HaRbor translOcaTions between The igH and C-mYc

recombination, may involv e DSBs and  thus , e mpl oy  NHE J. W e have shown t h at d efective NHEJ during a ttemp te d  V(D ) Jrecom binatio n  i n a p 53  t umo r- s up press ordeficie nt backgro und  r eproduciblyl ea ds to muri nepro-B cell l ymp homasth ath arbor  tr anslo cation s  betwe en the Ig Ha nd c-m y c

recombination,_may involve_DSBs and thus, employ_NHEJ. We_have_shown that_defective_NHEJ during attempted_V(D)J recombination in_a p53 tumor-suppressor deficient_background reproducibly leads_to_murine pro-B cell lymphomas that harbor translocations between the IgH and c-myc


course We also continued our studies on the G-protein coupled receptor for sphingosine-1-phosphate, S1P1. To determine the precise role of the S1P1 receptor on T-cells, we established a T-cell-specific S1P1 knock-out mouse. The mutant mice

course We also continued our studies on the G - protein pair sense organ for sphingosine-1 - phosphate, S1P1. To determine the precise function of the S1P1 sense organ on T - cells, we established a T - cellular telephone - specific S1P1 knock - out shiner. The mutant mice

cokrse We also continued omr studies on thg T-protemn coupmed receotor for sphingosine-1-phosphatx, S1P1. To dtnermine the precise rule of thv S1P1 receptor in T-cells, xs established w T-czlo-specific S1P1 kkock-out mouve. The mutant kize

course We also continued our studies on coupled for sphingosine-1-phosphate, To determine the receptor T-cells, we established T-cell-specific S1P1 knock-out The mutant mice

course We also continued our sTudies on thE G-proTeiN coUpLed rEcepTor for sphingosINe-1-phOsphate, S1P1. To determine thE precIsE Role OF tHe S1P1 rEceptor ON T-CELls, We EsTabLiSHeD a T-ceLl-sPecific s1P1 knock-out MouSe. the mutant micE

course We also continuedour studie s onthe  G- pr otei n co upled receptor forsphingosine-1-phosphat e, S1 P1 .  Tod et ermin e the p r ec i s e r ol eofth e  S 1P1 r ece ptor on  T-cells,wees tablished aT -c ell-specif icS1P1 knock-o utmouse.  T hem utant  mi ce

course_We also_continued our studies on_the G-protein_coupled_receptor for_sphingosine-1-phosphate,_S1P1. To determine_the precise role_of the S1P1 receptor_on T-cells, we_established_a T-cell-specific S1P1 knock-out mouse. The mutant mice


of antibiotic resistance may be ascribed to such heterogeneities. In either case, there is an enormous need to characterize the transcriptional dynamics at the resolution of individual microbial cell. However traditional approaches relying on one or a few selected reporter genes are inadequate for this challenge. Recent technical advances now allow us to use RNA-Seq to

of antibiotic resistance may be ascribed to such heterogeneities. In either case, there be an enormous motivation to characterize the transcriptional dynamics at the resolution of individual microbial cellular telephone. However traditional approaches relying on one or a few selected reporter gene are inadequate for this challenge. late technical advances now allow us to use RNA - Seq to

of antibiotic resistance mxy be ascribed jo such ieterogsneities. In either case, there is an xnornous beed to characterize tfe transcgiptional dynemics at the resolution of indjyiduan microbial celk. However draditional ap[ruaehes relying on one or a few selecteq reporyeg genes are inwdeqlaee fkg uhis challenge. Recent technical asvances now allow us to use RNA-Seq to

of antibiotic resistance may be ascribed to In case, there an enormous need at resolution of individual cell. However traditional relying on one or a few reporter genes are inadequate for this challenge. Recent technical advances now allow us use RNA-Seq to

of antibiotic resistance may Be ascribed To sucH heTerOgEneiTies. in either case, thERe is An enormous need to characTerizE tHE traNScRiptiOnal dynAMiCS At tHe ReSolUtIOn Of indIviDual micRobial cell. howEvEr traditionaL ApProaches reLyiNg on one or a feW seLected RePorTEr genEs aRe inaDequatE For thiS challengE. RECent teCHnical aDVAnCes nOw allow us to use RNA-sEq TO

of antibiotic resistancemay be asc ribed  to  su ch  het erog eneities. In e i ther  case, there is an eno rmous  n e ed t o  c harac terizet he t ran sc ri pti on a ldynam ics  at the  resolutio n o findividual m i cr obial cell . H owever tradi tio nal ap pr oac h es re lyi ng on  one o r  a few  selected  r e porter genes a r e  i nade quate for this ch a ll e nge. Recent te chnica la dv a n ces  no w allow us  t o use RNA-Seq to

of_antibiotic resistance_may be ascribed to_such heterogeneities._In_either case,_there_is an enormous_need to characterize_the transcriptional dynamics at_the resolution of_individual_microbial cell. However traditional approaches relying on one or a few selected reporter genes_are_inadequate for_this_challenge._Recent technical advances now allow_us to use RNA-Seq to


an inhibitor of protein tyrosine phosphatases known to change the natural history of MF in a Gata(low)'mouse model developed in project 4, and allogeneic stem cell transplant as a potential curative approach. In PV, the effects of pegylated interferon alpha-2a as well as anti-thrombotic agents

an inhibitor of protein tyrosine phosphatases known to change the natural history of MF in a Gata(low)'mouse exemplar modernize in project 4, and allogeneic stem cell graft as a potential curative approach. In PV, the effects of pegylated interferon alpha-2a equally well as anti - thrombotic agent

an inhibitor of protein tyvosine phosphatases knoxn to cgange thd natural history of MF in a Gqta(loq)'mouse model developed in projebt 4, and aologtneic stem cell tcznsplanb as z potznvial curative akproach. In PE, the effects mf pzgylated interferon alpha-2a as well af anti-tnrlmbotic agents

an inhibitor of protein tyrosine phosphatases known the history of in a Gata(low)'mouse and stem cell transplant a potential curative In PV, the effects of pegylated alpha-2a as well as anti-thrombotic agents

an inhibitor of protein tyrosIne phosphaTases KnoWn tO cHangE the Natural history OF MF iN a Gata(low)'mouse model devElopeD iN ProjECt 4, And alLogeneiC StEM CelL tRaNspLaNT aS a potEntIal curaTive approaCh. IN Pv, the effects oF PeGylated intErfEron alpha-2a as WelL as antI-tHroMBotic AgeNts

an inhibitor of protein t yrosine ph ospha tas eskn ownto c hange the natu r al h istory of MF in a Gata (low) 'm o usem od el de veloped in p roj ec t4,an d  a lloge nei c stemcell trans pla nt  as a potent i al  curativeapp roach. In PV , t he eff ec tso f peg yla ted i nterfe r on alp ha-2a aswe l l as a n ti-thro m b ot ic a gents

an_inhibitor of_protein tyrosine phosphatases known_to change_the_natural history_of_MF in a_Gata(low)'mouse model developed_in project 4, and_allogeneic stem cell_transplant_as a potential curative approach. In PV, the effects of pegylated interferon alpha-2a as_well_as anti-thrombotic_agents


NAATs is a critical barrier to timely diagnosis and treatment. PATH proposes a collaborative effort with the CDC that simultaneously merges scientific and technological capabilities with this clinical need to develop a calcium oxide (CaO)-heated DNA amplification kit that obviates the requirements for electricity and instrumentation at the point of care. PATH has

NAATs is a critical barrier to timely diagnosis and treatment. PATH proposes a collaborative effort with the CDC that simultaneously merges scientific and technical capability with this clinical need to develop a calcium oxide (CaO)-heated DNA amplification kit that obviate the necessity for electricity and instrumentation at the point of concern. PATH have

NAWTs is a critical barriev to timely diaguisis aid treafment. PAGH proposes a collaborative xffoet wiuk the CDC that simulganeously merges wcieitific and technological capabjpitizs with this clikical need do develop a cdlzibm oxide (CaO)-heated DNA amplification kit thst obviates the tequigeienta for electricity and instrumentatjon at uhe point of care. LATH has

NAATs is a critical barrier to timely treatment. proposes a effort with the and capabilities with this need to develop calcium oxide (CaO)-heated DNA amplification kit obviates the requirements for electricity and instrumentation at the point of care. PATH

NAATs is a critical barrier to Timely diagNosis And TreAtMent. pATH Proposes a collaBOratIve effort with the CDC thaT simuLtANeouSLy MergeS scientIFiC ANd tEcHnOloGiCAl CapabIliTies witH this cliniCal NeEd to develop a CAlCium oxide (CAO)-hEated DNA amplIfiCation KiT thAT obviAteS the rEquireMEnts foR electricItY And insTRumentaTIOn At thE point of care. PATH hAS

NAATs is a critical barri er to time ly di agn osi sandtrea tment. PATH pr o pose s a collaborative effo rt wi th theC DC  that  simult a ne o u sly  m er ges  s c ie ntifi c a nd tech nologicalcap ab ilities with th is clinica l n eed to devel opa calc iu m o x ide ( CaO )-hea ted DN A  ampli ficationki t  thato bviates t he  req uirements for ele c tr i city and instr umenta ti o na t  th e p oint of ca re . PAT H  has

NAATs_is a_critical barrier to timely_diagnosis and_treatment._PATH proposes_a_collaborative effort with_the CDC that_simultaneously merges scientific and_technological capabilities with_this_clinical need to develop a calcium oxide (CaO)-heated DNA amplification kit that obviates the_requirements_for electricity_and_instrumentation_at the point of care._PATH has


human anxiety and affective disorders, results that will be immediately relevant to children at risk for the development of psychopathology. This proposed project intends to address challenges identified by and realize the full potential of two earlier SEPA-funded projects: a project completed in 2012, Education for Community Genomic Awareness (Grant Code: R

human anxiety and affective disorders, results that will be immediately relevant to children at hazard for the growth of psychopathology. This proposed undertaking intends to cover challenges identified by and recognize the full potential of two early SEPA - funded projects: a undertaking completed in 2012, Education for Community Genomic Awareness (Grant Code: R

hulan anxiety and affectivt disorders, resuljs that xill be immediagely relevant to children at rusk fir the development of osychopatjology. Tyis kroposed project mhtends bj adsvess ehellenges identinied by and realize the fglu 'otential of two earlier SEPA-funded [rojectx: w project compjetec in 2012, Vdmcation for Community Genomic Awzreness (Grant Code: R

human anxiety and affective disorders, results that immediately to children risk for the project to address challenges by and realize full potential of two earlier SEPA-funded a project completed in 2012, Education for Community Genomic Awareness (Grant Code: R

human anxiety and affective dIsorders, reSults ThaT wiLl Be imMediAtely relevant tO ChilDren at risk for the develoPment Of PSychOPaTholoGy. This pROpOSEd pRoJeCt iNtENdS to adDreSs challEnges identIfiEd By and realize THe Full potentIal Of two earlier sEPa-fundeD pRojECts: a pRojEct coMpleteD In 2012, EducAtion for COmMUnity GENomic AwAREnEss (GRant Code: R

human anxiety and affecti ve disorde rs, r esu lts  t hatwill  be immediatel y  rel evant to children at r isk f or thed ev elopm ent ofp sy c h opa th ol ogy .T hi s pro pos ed proj ect intend s t oaddress chal l en ges identi fie d by and rea liz e thefu llp otent ial  of t wo ear l ier SE PA-funded  p r ojects :  a proj e c tcomp leted in 2012, Ed u ca t ion for Commun ity Ge no m ic A war ene ss (GrantCo de: R

human_anxiety and_affective disorders, results that_will be_immediately_relevant to_children_at risk for_the development of_psychopathology. This proposed project_intends to address_challenges_identified by and realize the full potential of two earlier SEPA-funded projects: a project_completed_in 2012,_Education_for_Community Genomic Awareness (Grant Code:_R


tendon-bone repair site can be accelerated by delivery, at the time of repair, of targeted gene products that enhance expression of growth factors that are important to early tissue healing. These include: basic fibroblast growth factor (bFGF), platelet- derived growth factor (PDGF) and vascular endothelial growth factor (VEGF).

tendon - bone repair site can be accelerated by delivery, at the meter of haunt, of targeted gene products that enhance formula of emergence factors that are important to early tissue healing. These admit: basic fibroblast growth factor (bFGF), platelet- derived increase factor (PDGF) and vascular endothelial growth gene (VEGF).

tejdon-bone repair site can be accelerated by delmvery, af the tioe of repair, of targeted genx priductw that enhance expresskon of grlwth facrors rhat are important to eadpy tnswue healing. Thgse include: tasic fibroblavt gxowth factor (bFGF), platelet- derived gwowth fschor (PDGF) and vwscukwr ehdothelial growth factor (VEGF).

tendon-bone repair site can be accelerated by the of repair, targeted gene products factors are important to tissue healing. These basic fibroblast growth factor (bFGF), platelet- growth factor (PDGF) and vascular endothelial growth factor (VEGF).

tendon-bone repair site can be AccelerateD by deLivEry, At The tIme oF repair, of targeTEd geNe products that enhance eXpresSiON of gROwTh facTors thaT ArE IMpoRtAnT to EaRLy TissuE heAling. ThEse include: BasIc Fibroblast grOWtH factor (bFGf), plAtelet- deriveD grOwth faCtOr (PdgF) and VasCular EndothELial grOwth factoR (VegF).

tendon-bone repair site c an be acce lerat edbyde live ry,at the time of repa ir, of targeted gene p roduc ts that en hance  expres s io n ofgr ow thfa c to rs th atare imp ortant toear ly  tissue heal i ng . These in clu de: basic fi bro blastgr owt h  fact or(bFGF ), pla t elet-derived g ro w th fac t or (PDG F )  a nd v ascular endotheli a lg rowth factor ( VEGF).

tendon-bone_repair site_can be accelerated by_delivery, at_the_time of_repair,_of targeted gene_products that enhance_expression of growth factors_that are important_to_early tissue healing. These include: basic fibroblast growth factor (bFGF), platelet- derived growth factor_(PDGF)_and vascular_endothelial_growth_factor (VEGF).


Foam cell formation and associated pro- inflammatory behavior involve transcriptional regulation of many genes, but the specific transcriptional regulatory mechanisms controlling these expression changes in response to various lesion-associated stimuli are not well understood. We will identify transcription factors that regulate foam cell formation using a systems biology approach. Murine macrophages will be stimulated with modified

Foam cell formation and associated pro- inflammatory demeanor imply transcriptional regulation of many genes, but the specific transcriptional regulative mechanisms master these expression changes in reaction to various lesion - associate stimuli are not well understood. We will name transcription factors that regulate foam cell constitution using a systems biology approach. Murine macrophages will be stimulate with modified

Fowm cell formation and asrociated pro- inyoammatmry begavior ivvolve transcriptional reguletiob of nany genes, but the spezific trajscriptiinal eegulatory mechanisms cohbrollnnj these expresslon changes in response tm xaxious lesion-associated stimuli are njt well ujderstood. We wyll pdqntirj uranscription factors that regulafe foam cell formatipn using a systems biology appgoach. Murine macroohages will be frimulated wigh modified

Foam cell formation and associated pro- inflammatory transcriptional of many but the specific expression in response to lesion-associated stimuli are well understood. We will identify transcription that regulate foam cell formation using a systems biology approach. Murine macrophages will stimulated with modified

Foam cell formation and assocIated pro- inFlammAtoRy bEhAvioR invOlve transcriptIOnal Regulation of many genes, bUt the SpECifiC TrAnscrIptionaL ReGULatOrY mEchAnISmS contRolLing theSe expressiOn cHaNges in responSE tO various leSioN-associated sTimUli are NoT weLL undeRstOod. We Will idENtify tRanscriptIoN FactorS That regULAtE foaM cell formation usiNG a SYstems biology aPproacH. MURiNE MacRopHages will bE sTimulATed with MOdIFIEd

Foam cell formation and a ssociatedpro-inf lam ma tory  beh avior involvet rans criptional regulationof ma ny gene s ,but t he spec i fi c tra ns cr ipt io n al  regu lat ory mec hanisms co ntr ol ling these e x pr ession cha nge s in respons e t o vari ou s l e sion- ass ociat ed sti m uli ar e not wel lu nderst o od. Wew i ll  ide ntify transcripti o nf actors that re gulate  f o am c ell  fo rmation us in g a s y stems b i ol o g y  ap p roach. Murine  macrophage s  wi ll best imu l ated w ith m od i fie d

Foam_cell formation_and associated pro- inflammatory_behavior involve_transcriptional_regulation of_many_genes, but the_specific transcriptional regulatory_mechanisms controlling these expression_changes in response_to_various lesion-associated stimuli are not well understood. We will identify transcription factors that regulate_foam_cell formation_using_a_systems biology approach. Murine macrophages_will be stimulated with modified


, 2) health policy and the guideline development process, and 3) determining the value of lung cancer screening from the patient and societal perspective. Findings from the symposium will inform strategies for research, policy, and implementation and evaluation of emerging lung cancer screening programs. We evaluated the effect of aging on fat and carbohydrate metabolism during

, 2) health policy and the guideline development process, and 3) determining the value of lung cancer screen from the affected role and societal perspective. finding from the symposium will inform strategy for research, policy, and implementation and evaluation of emerge lung cancer cover programs. We evaluated the effect of aging on fat and carbohydrate metamorphosis during

, 2) hfalth policy and the guiaeline developmgnr procxss, and 3) determkning the value of lung cancxr sxreenung from the patient avd societwl perspwctite. Findings from the symijsiuj wiln inform stratenies for revearch, policy, dna nmplementation and evaluation of emewging linh cancer screeging [rogdams. We evaluated the effect of agjng on hat and carbohycrate metabolism during

, 2) health policy and the guideline and determining the of lung cancer societal Findings from the will inform strategies research, policy, and implementation and evaluation emerging lung cancer screening programs. We evaluated the effect of aging on fat carbohydrate metabolism during

, 2) health policy and the guideliNe developmEnt prOceSs, aNd 3) DeteRminIng the value of lUNg caNcer screening from the paTient AnD SociETaL persPective. fInDINgs FrOm The SyMPoSium wIll Inform sTrategies fOr rEsEarch, policy, aND iMplementatIon And evaluatioN of EmergiNg LunG CanceR scReeniNg progRAms. We eValuated tHe EFfect oF Aging on FAT aNd caRbohydrate metabolISm DUring

, 2) health policy and the  guideline  deve lop men tproc ess,  and 3) determ i ning  the value of lung can cer s cr e enin g  f rom t he pati e nt a ndso ci eta lp er spect ive . Findi ngs from t hesy mposium will in form strat egi es for resea rch , poli cy , a n d imp lem entat ion an d  evalu ation ofem e rgingl ung can c e rscre ening programs. W e  e v aluated the ef fect o fa gi n g  on  fa t and carb oh ydrat e  metabo l is m d uri n g

, 2)_health policy_and the guideline development_process, and_3)_determining the_value_of lung cancer_screening from the_patient and societal perspective._Findings from the_symposium_will inform strategies for research, policy, and implementation and evaluation of emerging lung cancer_screening_programs. We_evaluated_the_effect of aging on fat_and carbohydrate metabolism during


neutralization will be studied, since exosomes have potential antigens, like RVG, and will need to be administrated often. The UH3 further establishes exosome-based therapeutics, by study of reversal or prevention of neuropathology and aberrant movement in HD mouse models. Dosing of exosomes will be secured. A team

neutralization will be studied, since exosomes have potential antigens, like RVG, and will necessitate to be administer often. The UH3 further prove exosome - base therapeutics, by study of about-face or prevention of neuropathology and aberrant apparent motion in HD mouse model. Dosing of exosomes will be secured. A team

neuhralization will be studled, since exosomgs have 'otentizl antigdns, like RVG, and will need tl ve adninistrated often. The JH3 furtheg establiwhes wxosome-basxs theraizutica, by vvudy of reversak or prevettion of neuro[aghllogy and aberrant movement in HD mjuse moceps. Dosing of evosokqs wjll be secured. A team

neutralization will be studied, since exosomes have like and will to be administrated exosome-based by study of or prevention of and aberrant movement in HD mouse Dosing of exosomes will be secured. A team

neutralization will be studiEd, since exoSomes HavE poTeNtiaL antIgens, like RVG, anD Will Need to be administrated oFten. THe uh3 furTHeR estaBlishes EXoSOMe-bAsEd TheRaPEuTics, bY stUdy of reVersal or prEveNtIon of neuropaTHoLogy and abeRraNt movement in hD mOuse moDeLs. DOSing oF exOsomeS will bE SecureD. A team

neutralization will be stu died, sinc e exo som esha ve p oten tial antigens, like  RVG, and will need to  be a dm i nist r at ed of ten. Th e  U H 3  fu rt he r e st a bl ishes  ex osome-b ased thera peu ti cs, by study of  reversalorprevention o f n europa th olo g y and  ab erran t move m ent in  HD mouse  m o dels.D osing o f ex osom es will be secure d .A  team

neutralization will_be studied,_since exosomes have potential_antigens, like_RVG,_and will_need_to be administrated_often. The UH3_further establishes exosome-based therapeutics,_by study of_reversal_or prevention of neuropathology and aberrant movement in HD mouse models. Dosing of exosomes_will_be secured._A_team


sites of storage or utilization. Properites of each of these events will be further examined. The effects of pH, ionic strength, lipoprotein charge and ACTH stimulation on binding will be characterized. The effects of energy inhibition on cholesterol uptake will be determined. We will determine if both esterified and free cholesterol as well as

sites of storage or utilization. Properites of each of these events will be far probe. The effects of pH, ionic strength, lipoprotein charge and ACTH foreplay on binding will be characterized. The effects of department of energy prohibition on cholesterol uptake will be determined. We will determine if both esterified and free cholesterol equally well as

sihes of storage or utilizxtion. Properites of earh of tgese evevts will be further examined. Tye efdects of pH, ionic strevgth, lipoirotein cyargt and ACTH stimulefion on bindihn wiln be characterieed. The effewts of energy hnfiyition on cholesterol uptake will be determonfd. We will detgrmint is bofh esterified and free cholesterol as weln as

sites of storage or utilization. Properites of these will be examined. The effects charge ACTH stimulation on will be characterized. effects of energy inhibition on cholesterol will be determined. We will determine if both esterified and free cholesterol as as

sites of storage or utilizatiOn. ProperitEs of eAch Of tHeSe evEnts Will be further eXAminEd. The effects of pH, ionic sTrengTh, LIpopROtEin chArge and acTh STimUlAtIon On BInDing wIll Be charaCterized. ThE efFeCts of energy iNHiBition on chOleSterol uptake WilL be detErMinED. We wiLl dEtermIne if bOTh esteRified and FrEE choleSTerol as WELl As

sites of storage or utili zation. Pr operi tes  of  e achof t hese events wi l l be  further examined. The  effe ct s  ofp H,  ioni c stren g th , lip op ro tei nc ha rge a ndACTH st imulationonbi nding will b e  c haracteriz ed.  The effects  of  energ yinh i bitio n o n cho lester o l upta ke will b ed etermi n ed. Wew i ll  det ermine if both es t er i fied and freecholes te r ol a s w ell  as

sites_of storage_or utilization. Properites of_each of_these_events will_be_further examined. The_effects of pH,_ionic strength, lipoprotein charge_and ACTH stimulation_on_binding will be characterized. The effects of energy inhibition on cholesterol uptake will be_determined._We will_determine_if_both esterified and free cholesterol_as well as


in the cell cycle, including the expression of p53, the retinoblastoma suppressor protein (pRb), and enzymes directly required for initiating DNA synthesis. We anticipate that the results of these experiments will shed further light on both the mechanisms generating increased levels of guanine nucleotides in proliferating cells and the sequelae of reductions in

in the cell cycle, including the expression of p53, the retinoblastoma suppressor protein (pRb), and enzyme immediately want for initiating DNA deduction. We anticipate that the resultant role of these experiments will shed further luminosity on both the mechanisms generating increase levels of guanine nucleotides in proliferate cells and the sequelae of reduction in

in the cell cycle, includinn the expression of p53, tie retihoblastooa suppressor protein (pRb), anv enzymes directly required for initiatijg DNA stnthtsis. We anticipatx that tmz reshpts mh these experimgnts will shad further ligvt ou both the mechanisms generating incweased kegels of guaning nuckqotisvs in proliferating cells and tge sequtlae of reductions in

in the cell cycle, including the expression the suppressor protein and enzymes directly We that the results these experiments will further light on both the mechanisms increased levels of guanine nucleotides in proliferating cells and the sequelae of reductions

in the cell cycle, including thE expressioN of p53, tHe rEtiNoBlasToma Suppressor protEIn (pRB), and enzymes directly reqUired FoR InitIAtIng DNa syntheSIs. wE AntIcIpAte ThAT tHe resUltS of thesE experimenTs wIlL shed further LIgHt on both thE meChanisms geneRatIng incReAseD LevelS of GuaniNe nuclEOtides In prolifeRaTIng celLS and the SEQuElae Of reductions in

in the cell cycle, includ ing the ex press ion  of  p 53,theretinoblastoma supp ressor protein (pRb),and e nz y mesd ir ectly  requir e df o r i ni ti ati ng DN A syn the sis. We  anticipat e t ha t the result s  o f these ex per iments willshe d furt he r l i ght o n b oth t he mec h anisms  generati ng increa s ed leve l s  o f gu anine nucleotides in proliferatingcellsan d  t h e  se que lae of red uc tions in

in_the cell_cycle, including the expression_of p53,_the_retinoblastoma suppressor_protein_(pRb), and enzymes_directly required for_initiating DNA synthesis. We_anticipate that the_results_of these experiments will shed further light on both the mechanisms generating increased levels_of_guanine nucleotides_in_proliferating_cells and the sequelae of_reductions in


and specimens for the scientific community. This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution

and specimens for the scientific community. This subproject is one of many research subprojects utilizing the resource provide by a Center grant funded by NIH / NCRR. The subproject and investigator (PI) may have receive primary funding from another NIH reservoir, and therefore could be represented in other CRISP entries. The institution

anf specimens for the sciektific community. This snbprojedt is ond of many research subprojecvs urilizung the resources provkded by a Center tranu funded by NIH/NCCD. The smyprojsgt anb mnvestigator (PI) may have seceived primasy fbnding from another NIH source, and trus coukd be representeq in jthed CRISP entries. The institution

and specimens for the scientific community. This one many research utilizing the resources funded NIH/NCRR. The subproject investigator (PI) may received primary funding from another NIH and thus could be represented in other CRISP entries. The institution

and specimens for the scientiFic communiTy. ThiS suBprOjEct iS one Of many research SUbprOjects utilizing the resoUrces PrOVideD By A CentEr grant FUnDED by nIh/NcRR. thE SuBprojEct And inveStigator (PI) May HaVe received prIMaRy funding fRom Another NIH soUrcE, and thUs CouLD be rePreSenteD in othER CRISP Entries. ThE iNStitutIOn

and specimens for the sci entific co mmuni ty.  Th is  sub proj ect is one ofm anyresearch subprojects u tiliz in g  the re sourc es prov i de d byaCe nte rg ra nt fu nde d by NI H/NCRR. Th e s ub project andi nv estigator(PI ) may have r ece ived p ri mar y  fund ing  from  anoth e r NIHsource, a nd thus c o uld ber e pr esen ted in other CRIS P  e n tries. The ins tituti on

and_specimens for_the scientific community. This_subproject is_one_of many_research_subprojects utilizing the_resources provided by_a Center grant funded_by NIH/NCRR. The_subproject_and investigator (PI) may have received primary funding from another NIH source, and thus_could_be represented_in_other_CRISP entries. The institution


prepare reports. The Administrative core consists of the two SPORE principal investigators, Drs. Bunn and Miller; the SPORE executive director. Donna Berrier, a 40% grants manager/administrative assistant and 10% financial manager. This core will also develop and circulate research conference schedules, coordinate scientific review,

prepare reports. The Administrative core consists of the two SPORE chief detective, Drs. Bunn and Miller; the SPORE executive director. Donna Berrier, a 40% grant coach / administrative assistant and 10% financial manager. This kernel will also develop and go around research conference schedule, coordinate scientific review,

prfpare reports. The Adminirtrative core consists of ths two SPURE principal investigators, Vrs. Vunn qnd Miller; the SPORE ebecutive firector. Donia Berrier, a 40% grehts manager/adjlnistxavive assistant snd 10% finanwial manager. Tvir eore will also develop and circulate researvh conference scredukqs, ckordinate scientific review,

prepare reports. The Administrative core consists of SPORE investigators, Drs. and Miller; the a grants manager/administrative assistant 10% financial manager. core will also develop and circulate conference schedules, coordinate scientific review,

prepare reports. The AdministRative core ConsiSts Of tHe Two SpORE Principal invesTIgatOrs, Drs. Bunn and Miller; the sPORE ExECutiVE dIrectOr. Donna bErRIEr, a 40% GrAnTs mAnAGeR/admiNisTrative Assistant aNd 10% fInAncial manageR. thIs core will AlsO develop and cIrcUlate rEsEarCH confEreNce scHeduleS, CoordiNate scienTiFIc reviEW,

prepare reports. The Admi nistrative  core  co nsi st s of  the  two SPORE pri n cipa l investigators, Drs.Bunnan d  Mil l er ; the  SPOREe xe c u tiv edi rec to r .Donna  Be rrier,a 40% gran tsma nager/admini s tr ative assi sta nt and 10% f ina ncialma nag e r. Th iscorewill a l so dev elop andci r culate researc h co nfer ence schedules, c o or d inate scientif ic rev ie w ,

prepare_reports. The_Administrative core consists of_the two_SPORE_principal investigators,_Drs._Bunn and Miller;_the SPORE executive_director. Donna Berrier, a_40% grants manager/administrative_assistant_and 10% financial manager. This core will also develop and circulate research conference schedules,_coordinate_scientific review,


of this proposal is to enable a new capability to isolate and concentrate biomarkers from blood in small volumes (200 microliters), at high sensitivity, over short periods of time, and to monitor multiple markers. We initially will focus on biomarkers for myocardial infarction and stroke. Upon completion of the entire project-which this proposal enables

of this proposal is to enable a new capability to isolate and concentrate biomarkers from rake in humble volumes (200 microliters), at high sensitivity, over short period of time, and to monitor multiple markers. We initially will concenter on biomarkers for myocardial infarct and stroke. Upon completion of the entire project - which this proposal enables

of this proposal is to enanle a new capability to isolafe and cuncentrate biomarkers from bpoid in small volumes (200 microlkters), at jigh senwititity, over short 'sriods of tims, and vo monitor multlple markerv. We initially wklp focus on biomarkers for myocardiaj infarvtlon and stroke. Upom comlletion of the entire project-which this pgoposal enables

of this proposal is to enable a to and concentrate from blood in high over short periods time, and to multiple markers. We initially will focus biomarkers for myocardial infarction and stroke. Upon completion of the entire project-which this enables

of this proposal is to enable a New capabilIty to IsoLatE aNd coNcenTrate biomarkerS From Blood in small volumes (200 micRolitErS), At hiGH sEnsitIvity, ovER sHORt pErIoDs oF tIMe, And to MonItor mulTiple markeRs. WE iNitially will FOcUs on biomarKerS for myocardiAl iNfarctIoN anD StrokE. UpOn comPletioN Of the eNtire projEcT-Which tHIs propoSAL eNablEs

of this proposal is to en able a new  capa bil ity  t o is olat e and concentr a te b iomarkers from blood i n sma ll volu m es  (200  microl i te r s ),at  h igh  s e ns itivi ty,  over s hort perio dsof  time, and t o  m onitor mul tip le markers.Weinitia ll y w i ll fo cus  on b iomark e rs for  myocardi al infarc t ion and s tr oke.  Upon completiono ft he entire proj ect-wh ic h  t h i s p rop osal enabl es

of_this proposal_is to enable a_new capability_to_isolate and_concentrate_biomarkers from blood_in small volumes_(200 microliters), at high_sensitivity, over short_periods_of time, and to monitor multiple markers. We initially will focus on biomarkers for_myocardial_infarction and_stroke._Upon_completion of the entire project-which_this proposal enables


Our goal is to continue monitoring our subject population of 3,020 individuals so that risk factors for the development of chronic back pain disability can be identified. Our efforts to establish this data base have been supported to date by NIOSH; however, NIOSH has stated that the evaluation of chronic disability is beyond their scope

Our goal is to continue monitoring our subject population of 3,020 person so that hazard factors for the development of chronic binding annoyance disability can be identified. Our efforts to establish this datum nucleotide have been supported to date by NIOSH; however, NIOSH has state that the evaluation of chronic disability is beyond their scope

Oug goal is to continue mokitoring our sublwct po'ulatioh of 3,020 inaividuals so that risk factocs fir tht development of chfonic bacn pain dusabmlity can be ideifified. Our efrlrts vo establish thls data basa have been su[purced to date by NIOSH; however, NIOSH hws statrd that the evaloatiom of dhronic disability is beyond their scope

Our goal is to continue monitoring our of individuals so risk factors for pain can be identified. efforts to establish data base have been supported to by NIOSH; however, NIOSH has stated that the evaluation of chronic disability is their scope

Our goal is to continue monitoRing our subJect pOpuLatIoN of 3,020 iNdivIduals so that riSK facTors for the development oF chroNiC Back PAiN disaBility cAN bE IDenTiFiEd. OUr EFfOrts tO esTablish This data baSe hAvE been supportED tO date by NIOsH; hOwever, NIOSH hAs sTated tHaT thE EvaluAtiOn of cHronic DIsabilIty is beyoNd THeir scOPe

Our goal is to continue m onitoringour s ubj ect  p opul atio n of 3,020 ind i vidu als so that risk facto rs fo rt he d e ve lopme nt of c h ro n i c b ac kpai nd is abili tycan beidentified . O ur  efforts toe st ablish thi s d ata base hav e b een su pp ort e d todat e byNIOSH; howeve r, NIOSHha s  state d  that t h e  e valu ation of chronicd is a bility is beyo nd the ir sc o p e

Our_goal is_to continue monitoring our_subject population_of_3,020 individuals_so_that risk factors_for the development_of chronic back pain_disability can be_identified._Our efforts to establish this data base have been supported to date by NIOSH;_however,_NIOSH has_stated_that_the evaluation of chronic disability_is beyond their scope


, chemical, and biological constants needed will be determined by simple experiments. Solution of the equations will be facilitated by a program for a large digital computer. To verify the pharmacokinetic model, the experimental conditions of suitable published studies in man will be simulated, and their results compared with the calculated data. Further verification will be done by

, chemical, and biological constants needed will be determined by simple experiment. Solution of the equation will be facilitated by a program for a large digital calculator. To verify the pharmacokinetic model, the experimental conditions of desirable publish studies in man will be simulated, and their results compare with the calculated data. Further confirmation will be done by

, chfmical, and biological cokstants needed wnol be vetermihed by skmple experiments. Solution oh thw equqtions will be facilitxted by a program for q large dijjtal computer. Bo vexihy the pharmacolinetic mogel, the experikevtcl conditions of suitable published ftudies ij man will be fimukwted, and their results compared with tge calcllated data. Furthrr verification will be doje bj

, chemical, and biological constants needed will by experiments. Solution the equations will for large digital computer. verify the pharmacokinetic the experimental conditions of suitable published in man will be simulated, and their results compared with the calculated data. verification will be done by

, chemical, and biological consTants needeD will Be dEteRmIned By siMple experimentS. soluTion of the equations will Be facIlITateD By A progRam for a LArGE DigItAl ComPuTEr. to verIfy The pharMacokinetiC moDeL, the experimeNTaL conditionS of Suitable publIshEd studIeS in MAn wilL be SimulAted, anD Their rEsults comPaREd with THe calcuLATeD datA. Further verificatIOn WIll be done by

, chemical, and biological  constants  need edwil lbe d eter mined by simpl e  exp eriments. Solution ofthe e qu a tion s  w ill b e facil i ta t e d b yapro gr a mfor a  la rge dig ital compu ter .To verify th e  p harmacokin eti c model, the  ex perime nt alc ondit ion s ofsuitab l e publ ished stu di e s in m a n willb e  s imul ated, and their r e su l ts compared wi th the  c a lc u l ate d d ata. Furth er  veri f ication wi l l bed one by

, chemical,_and biological_constants needed will be_determined by_simple_experiments. Solution_of_the equations will_be facilitated by_a program for a_large digital computer._To_verify the pharmacokinetic model, the experimental conditions of suitable published studies in man will_be_simulated, and_their_results_compared with the calculated data._Further verification will be done_by


c) no radiation, TP food, and d) 3 Gy, TP food. For C3H mice, one additional group was added receiving 3 Gy total body irradiation where the administration of the TP containing food was delayed one month post-irradiation. Preliminary data show TP food supplementation after radiation: a) did

c) no radiation, TP food, and d) 3 Gy, TP food. For C3H mice, one additional group was lend get 3 Gy total body irradiation where the government of the TP containing food was delayed one calendar month post - irradiation. Preliminary data display TP food supplementation after radiation: a) dress

c) jo radiation, TP food, and d) 3 Gy, TP food. Yir C3H kice, ohe additkonal group was added receivmng 3 Gy tital body irradiation dhere the adminisrratmon of the TP coifaining food sws dzleyed one month kost-irradiathon. Preliminarf aaca show TP food supplementation aftew radiayiln: a) did

c) no radiation, TP food, and d) TP For C3H one additional group total irradiation where the of the TP food was delayed one month post-irradiation. data show TP food supplementation after radiation: a) did

c) no radiation, TP food, and d) 3 Gy, Tp food. For C3H Mice, oNe aDdiTiOnal GrouP was added receiVIng 3 GY total body irradiation wHere tHe ADminIStRatioN of the Tp CoNTAinInG fOod WaS DeLayed One Month poSt-irradiatIon. prEliminary datA ShOw TP food suPplEmentation afTer RadiatIoN: a) dID

c) no radiation, TP food,  and d) 3Gy, T P f ood .ForC3Hmice, one addi t iona l group was added rece iving  3 Gy t o ta l bod y irrad i at i o n w he re  th ea dm inist rat ion ofthe TP con tai ni ng food wasd el ayed one m ont h post-irrad iat ion. P re lim i narydat a sho w TP f o od sup plementat io n  after radiati o n :a) d id

c)_no radiation,_TP food, and d)_3 Gy,_TP_food. For_C3H_mice, one additional_group was added_receiving 3 Gy total_body irradiation where_the_administration of the TP containing food was delayed one month post-irradiation. Preliminary data show_TP_food supplementation_after_radiation:_a) did


research: disparities in oral health and health care. Combining extant dental data with other components of the rich VA patient databases, we will be able to examine the effectiveness of fluoride in preventing caries in patients with five of the 14 priority conditions specified by AHRQ: medically compromised veterans with dementia, depression and bipolar disorder, diabetes

research: disparities in oral health and health care. Combining extant dental datum with early component of the rich VA patient databases, we will be able to probe the effectiveness of fluoride in preventing cavity in patients with five of the 14 priority conditions stipulate by AHRQ: medically compromised veterans with dementia, depressive disorder and bipolar disorder, diabetes

redearch: disparities in orxl health and hgaoth cace. Combjning exgant dental data with other rompinentw of the rich VA patievt databades, we wull ue able to examiis the enyectibcness if fluoride in preventinc caries in padidncs with five of the 14 priority condityons sprclfied by AHRQ: iedibajly dompromised veterans with dementia, depresvion and bipokar disorder, diabetes

research: disparities in oral health and health extant data with components of the will able to examine effectiveness of fluoride preventing caries in patients with five the 14 priority conditions specified by AHRQ: medically compromised veterans with dementia, depression bipolar disorder, diabetes

research: disparities in oral Health and hEalth CarE. CoMbIninG extAnt dental data wITh otHer components of the rich vA patIeNT datABaSes, we Will be aBLe TO ExaMiNe The EfFEcTivenEss Of fluorIde in preveNtiNg Caries in patiENtS with five oF thE 14 priority conDitIons spEcIfiED by AHrQ: mEdicaLly comPRomiseD veterans WiTH demenTIa, depreSSIoN and Bipolar disorder, diABeTEs

research: disparities inoral healt h and  he alt hcare . Co mbining extant dent al data with other com ponen ts of t h erichVA pati e nt d ata ba se s,we wi ll be  ab le to e xamine the  ef fe ctiveness of fl uoride inpre venting cari esin pat ie nts withfiv e ofthe 14 priori ty condit io n s spec i fied by A HR Q: m edically compromi s ed veterans withdement ia ,  d e p res sio n and bipo la r dis o rder, d i ab e t e s

research:_disparities in_oral health and health_care. Combining_extant_dental data_with_other components of_the rich VA_patient databases, we will_be able to_examine_the effectiveness of fluoride in preventing caries in patients with five of the 14_priority_conditions specified_by_AHRQ:_medically compromised veterans with dementia,_depression and bipolar disorder, diabetes


the superior EAM would improve virologic suppression by enabling real-time interventions to support ART adherence. In this randomized controlled trial, we will compare MyTPill to WisePill among N=80 HIV+ men/women taking prescription opioids and once-daily ART regimens containing tenofovir and emtricit

the superior EAM would improve virologic suppression by enabling veridical - meter interventions to support ART adhesiveness. In this randomized controlled test, we will compare MyTPill to WisePill among N=80 HIV+ world / women learn prescription opioids and once - daily artwork regimens containing tenofovir and emtricit

thf superior EAM would impvove virologic soppressimn by snabling real-time interventions to snppoet ARU adherence. In this randomizvd controoled rrial, we wmml compare MyFIill co WisePill amonn N=80 HIV+ men/fomen taking psercxiption opioids and once-daily ART redimens vojtaining tenofjvir wnd smtricit

the superior EAM would improve virologic suppression real-time to support adherence. In this compare to WisePill among HIV+ men/women taking opioids and once-daily ART regimens containing and emtricit

the superior EAM would improvE virologic SupprEssIon By EnabLing Real-time intervENtioNs to support ART adherencE. In thIs RAndoMIzEd conTrolled TRiAL, We wIlL cOmpArE mytPill To WIsePill Among N=80 HIV+ mEn/wOmEn taking presCRiPtion opioiDs aNd once-daily ArT rEgimenS cOntAIning TenOfoviR and emTRicit

the superior EAM would im prove viro logic  su ppr es sion  byenabling real- t imeinterventions to suppo rt AR Ta dher e nc e. In  this r a nd o m ize dco ntr ol l ed  tria l,we will  compare M yTP il l to WisePil l  a mong N=80HIV + men/womentak ing pr es cri p tionopi oidsand on c e-dail y ART reg im e ns con t ainingt e no fovi r and emtricit

the_superior EAM_would improve virologic suppression_by enabling_real-time_interventions to_support_ART adherence. In_this randomized controlled_trial, we will compare_MyTPill to WisePill_among_N=80 HIV+ men/women taking prescription opioids and once-daily ART regimens containing tenofovir and emtricit


under related projects in this Section.) We found that JCV DNA could be detected in 33 of 46 (72%) of sections from PML blocks. We have also found that JCV sequences could be detected in a paraffin section which had been scraped from slides following immunocytochemical staining. Detection of JCV in that

under related projects in this Section .) We found that JCV DNA could be detect in 33 of 46 (72 %) of section from PML blocks. We have also establish that JCV sequence could be detected in a paraffin part which had been scraped from slides come immunocytochemical staining. Detection of JCV in that

unfer related projects in uhis Section.) We found thet JCV SNA coula be detected in 33 of 46 (72%) of sertiobs frim PML blocks. We have xlso founf that JXV stquences could be detected in a iarafyii section which had been vcraped from sniaed following immunocytochemical staiging. Deyeftion of JCV ig thse

under related projects in this Section.) We JCV could be in 33 of PML We have also that JCV sequences be detected in a paraffin section had been scraped from slides following immunocytochemical staining. Detection of JCV in that

under related projects in thiS Section.) We Found ThaT JCv DnA coUld bE detected in 33 of 46 (72%) oF SectIons from PML blocks. We havE also FoUNd thAT JcV seqUences cOUlD BE deTeCtEd iN a PArAffin SecTion whiCh had been sCraPeD from slides fOLlOwing immunOcyTochemical stAinIng. DetEcTioN Of JCV In tHat

under related projects in  this Sect ion.)  We  fo un d th at J CV DNA could b e  det ected in 33 of 46 (72% ) ofse c tion s  f rom P ML bloc k s. W e h av eals of ou nd th atJCV seq uences cou ldbe  detected in aparaffin s ect ion which ha d b een sc ra ped fromsli des f ollowi n g immu nocytoche mi c al sta i ning. D e t ec tion  of JCV in that

under_related projects_in this Section.) We_found that_JCV_DNA could_be_detected in 33_of 46 (72%)_of sections from PML_blocks. We have_also_found that JCV sequences could be detected in a paraffin section which had been_scraped_from slides_following_immunocytochemical_staining. Detection of JCV in_that


liver and muscle is encoded by a single gene; its expression, however, is under separate genetic control. Patients with various clinical and enzymatic subgroups of Type III glycogen storage disease are being followed at Duke Medical Center; fibroblasts from these patients have been collected and stored. The debranching enzyme has been purified from porcine muscle and

liver and muscle is encoded by a single gene; its expression, however, is under freestanding familial control. Patients with various clinical and enzymatic subgroup of Type III glycogen storage disease are being followed at Duke Medical Center; fibroblast from these patients have been collected and stored. The debranching enzyme has been purify from porcine muscle and

liger and muscle is encodea by a single ggnw; its xxpressjon, howexer, is under separate genetir cobtrol. Patients with various clinical and enztmatmc subgroups of Vgpe III glycofcn stmcage disease arg being follmwed at Duke Madkccl Center; fibroblasts from these patyents hsvf been collectgd anc stodvd. The debranching enzyme has bsen purpfied from porcinr muscle and

liver and muscle is encoded by a its however, is separate genetic control. enzymatic of Type III storage disease are followed at Duke Medical Center; fibroblasts these patients have been collected and stored. The debranching enzyme has been purified porcine muscle and

liver and muscle is encoded by A single genE; its eXprEssIoN, howEver, Is under separatE GeneTic control. Patients with VarioUs CLiniCAl And enZymatic SUbGROupS oF TYpe iIi GlYcogeN stOrage diSease are beIng FoLlowed at Duke mEdIcal Center; FibRoblasts from TheSe patiEnTs hAVe beeN coLlectEd and sTOred. ThE debranchInG Enzyme HAs been pURIfIed fRom porcine muscle aND

liver and muscle is encod ed by a si nglegen e;it s ex pres sion, however, is u nder separate geneticcontr ol .  Pat i en ts wi th vari o us c lin ic al  an de nz ymati c s ubgroup s of TypeIII  g lycogen stor a ge  disease a rebeing follow edat Duk eMed i cal C ent er; f ibrobl a sts fr om thesepa t ientsh ave bee n co llec ted and stored. T h ed ebranching enz yme ha sb ee n pur ifi ed from po rc ine m u scle an d

liver_and muscle_is encoded by a_single gene;_its_expression, however,_is_under separate genetic_control. Patients with_various clinical and enzymatic_subgroups of Type_III_glycogen storage disease are being followed at Duke Medical Center; fibroblasts from these patients_have_been collected_and_stored._The debranching enzyme has been_purified from porcine muscle and


In order to determine whether the SFFV envelope glycoprotein is activating the Epo signal transduction pathway, studies were carried out to compare erythroid cells stimulated with Epo or infected with SFFV for expression of tyrosine- phosphorylated proteins and DNA-binding proteins. Both Epo and SFFV were shown to induce the

In order to determine whether the SFFV envelope glycoprotein is activating the Epo signal transduction pathway, report were carry out to compare erythroid cells stimulated with Epo or infected with SFFV for formulation of tyrosine- phosphorylated proteins and DNA - tie down proteins. Both Epo and SFFV were shown to induce the

In order to determine whetmer the SFFV envglipe glbcoprotsin is aztivating the Epo signal traisduxtion pathway, studies were zarried olt to compare wrythroid rslls stliulafcd wich Epo or infectgd with SFFV for expressiot uf tyrosine- phosphorylated proteins agd DNA-bonfing proteins. Foth Qpo znd SFFV were shown to induce the

In order to determine whether the SFFV is the Epo transduction pathway, studies erythroid stimulated with Epo infected with SFFV expression of tyrosine- phosphorylated proteins and proteins. Both Epo and SFFV were shown to induce the

In order to determine whether The SFFV envElope GlyCopRoTein Is acTivating the Epo SIgnaL transduction pathway, stUdies WeRE carRIeD out tO comparE ErYTHroId CeLls StIMuLated WitH Epo or iNfected witH SFfV For expressioN Of Tyrosine- phOspHorylated proTeiNs and DnA-BinDIng prOteIns. BoTh Epo aND SFFV wEre shown tO iNDuce thE

In order to determine whe ther the S FFV e nve lop eglyc opro tein is activa t ingthe Epo signal transdu ction  p a thwa y ,studi es were ca r r ied  o ut  to  c o mp are e ryt hroid c ells stimu lat ed  with Epo or in fected wit h S FFV for expr ess ion of  t yro s ine-pho sphor ylated protei ns and DN A- b inding protein s .  B othEpo and SFFV were sh o wn to induce t he

In_order to_determine whether the SFFV_envelope glycoprotein_is_activating the_Epo_signal transduction pathway,_studies were carried_out to compare erythroid_cells stimulated with_Epo_or infected with SFFV for expression of tyrosine- phosphorylated proteins and DNA-binding proteins. Both_Epo_and SFFV_were_shown_to induce the


's Project,1 and clinical samples in P. Ciborowski's Project 3, these nonhuman primate studies will form the necessary bridge between the projects as well as allow us to directly assess, in the most accessible and valid system, the target structures in the brain. Given the growing epidemic of METH use

's Project,1 and clinical samples in P. Ciborowski's Project 3, these nonhuman primate studies will form the necessary bridge between the projects equally well as leave us to directly assess, in the about accessible and valid system, the aim structures in the brain. give the growing epidemic of METH use

's Pgoject,1 and clinical sampues in P. Ciborocwki's Pcoject 3, these nunhuman primate studies will firm tye necessary bridge begween the projectw as qell as allow us to diredbly avwess, in the moxt accessitle and valid vyrtzm, the target structures in the braig. Given tje growing epiqemib jf MSNH use

's Project,1 and clinical samples in P. 3, nonhuman primate will form the as as allow us directly assess, in most accessible and valid system, the structures in the brain. Given the growing epidemic of METH use

's Project,1 and clinical sampleS in P. CiboroWski's proJecT 3, tHese NonhUman primate stuDIes wIll form the necessary briDge beTwEEn thE PrOjectS as well AS aLLOw uS tO dIreCtLY aSsess, In tHe most aCcessible aNd vAlId system, the tARgEt structurEs iN the brain. GivEn tHe growInG epIDemic Of MeTH usE

's Project,1 and clinicalsamples in  P. C ibo row sk i'sProj ect 3, these n o nhum an primate studies wil l for mt he n e ce ssary  bridge be t w een  t he  pr oj e ct s aswel l as al low us todir ec tly assess,i nthe most a cce ssible and v ali d syst em , t h e tar get  stru ctures in the  brain. G iv e n theg rowinge p id emic  of METH use

's Project,1_and clinical_samples in P. Ciborowski's_Project 3,_these_nonhuman primate_studies_will form the_necessary bridge between_the projects as well_as allow us_to_directly assess, in the most accessible and valid system, the target structures in the_brain._Given the_growing_epidemic_of METH use


glial interactions characteristic of schwannomas. We plan to use a combination of gain-of-function and loss-of-function mouse models and primary cells from human schwannomas to test these hypotheses. We believe that analysis of primary schwannoma cells offers a rare opportunity to study a pure population

glial interactions characteristic of schwannomas. We plan to use a combination of gain - of - affair and personnel casualty - of - function mouse models and elementary cells from human schwannomas to test these hypothesis. We believe that analysis of chief schwannoma cells offers a rare opportunity to learn a pure population

gliwl interactions charactevistic of schwanuimas. Wx plan fo use a combination of gain-of-functiln and ooss-of-function mouse mudels and primary celow from humeh schwakuomas bo tevv these hypothexes. We belheve that analfsks of primary schwannoma cells offers a rare ooportunity to ftudj w pudv kopulation

glial interactions characteristic of schwannomas. We plan a of gain-of-function loss-of-function mouse models schwannomas test these hypotheses. believe that analysis primary schwannoma cells offers a rare to study a pure population

glial interactions characteRistic of scHwannOmaS. We PlAn to Use a Combination of gAIn-of-Function and loss-of-functIon moUsE ModeLS aNd priMary celLS fROM huMaN sChwAnNOmAs to tEst These hyPotheses. We BelIeVe that analysIS oF primary scHwaNnoma cells ofFerS a rare OpPorTUnity To sTudy a Pure poPUlatioN

glial interactions charact eristic of  schw ann oma s.  Weplan  to use a comb i nati on of gain-of-function  andlo s s-of - fu nctio n mouse mo d e lsan dpri ma r ycells  fr om huma n schwanno mas  t o test these hy potheses.Webelieve that  an alysis  o f p r imary  sc hwann oma ce l ls off ers a rar eo pportu n ity tos t ud y apure population

glial interactions_characteristic of_schwannomas. We plan to_use a_combination_of gain-of-function_and_loss-of-function mouse models_and primary cells_from human schwannomas to_test these hypotheses._We_believe that analysis of primary schwannoma cells offers a rare opportunity to study a_pure_population


be defined in the neural lobe by determining by HPLC the ratios of the oxytocin neurophysins throughout density gradient granule separations in neural lobes from control, 1, 2, 3 and 5 day dehydrated rats. A similar definition of compartmentation in the sinus gland will be based upon HPLC determination of the partial

be defined in the neural lobe by determining by HPLC the ratios of the oxytocin neurophysins throughout concentration gradient granule legal separation in neural lobe from dominance, 1, 2, 3 and 5 day dehydrated informer. A like definition of compartmentation in the sinus gland will be based upon HPLC determination of the partial

be defined in the neural lube by determinnbg by IPLC ths ratios of the oxytocin neurophysind rhrouthout density gradient granule deparatiins mn neural lobes hdom conbxol, 1, 2, 3 and 5 day dehydratec rats. A shmilar definithov lf compartmentation in the sinus glwnd wilk he based upon RPLC qetedmination of the partial

be defined in the neural lobe by HPLC ratios of oxytocin neurophysins throughout neural from control, 1, 3 and 5 dehydrated rats. A similar definition of in the sinus gland will be based upon HPLC determination of the partial

be defined in the neural lobe bY determiniNg by HpLC The RaTios Of thE oxytocin neuroPHysiNs throughout density graDient GrANule SEpAratiOns in neURaL LObeS fRoM coNtROl, 1, 2, 3 And 5 daY deHydrateD rats. A simiLar DeFinition of coMPaRtmentatioN in The sinus glanD wiLl be baSeD upON HPLC DetErminAtion oF The parTial

be defined in the neurallobe by de termi nin g b yHPLC  the  ratios of the oxyt ocin neurophysins thro ughou td ensi t ygradi ent gra n ul e sep ar at ion si nneura l l obes fr om control , 1 ,2, 3 and 5 d a ydehydrated  ra ts. A simila r d efinit io n o f  comp art menta tion i n  the s inus glan dw ill be based u p o nHPLC  determination of th e  partial

be_defined in_the neural lobe by_determining by_HPLC_the ratios_of_the oxytocin neurophysins_throughout density gradient_granule separations in neural_lobes from control,_1,_2, 3 and 5 day dehydrated rats. A similar definition of compartmentation in the_sinus_gland will_be_based_upon HPLC determination of the_partial


study will look to see if there are differences in these factors between Hispanics and non-Hispanics and based on those differences conduct a trial to increase physical activity specific for Hispanic breast cancer survivors. The continuation of work in this area has revolved about the analysis of lymphocyte responsiveness in vitro to a putative suppressive

study will look to see if there are dispute in these divisor between Hispanics and non - Hispanics and based on those differences conduct a test to increase physical activity specific for Hispanic breast cancer survivor. The continuation of work in this sphere has roll about the analysis of lymphocyte responsiveness in vitro to a putative suppressive

stkdy will look to see if uhere are differeuxes in these factors between Hispanics and non-Hidpqnics and based on those diwferences conduct a tcial to increase physical actiblty s'erific for Hispakic breast wancer survivoss. Tke continuation of work in this area has refopved about the anakrsis of lymphocyte responsiveness in vjtro to a putative sippressive

study will look to see if there in factors between and non-Hispanics and a to increase physical specific for Hispanic cancer survivors. The continuation of work this area has revolved about the analysis of lymphocyte responsiveness in vitro to putative suppressive

study will look to see if there Are differeNces iN thEse FaCtorS betWeen Hispanics aND non-hispanics and based on thoSe difFeREnceS CoNduct A trial tO InCREasE pHySicAl ACtIvity SpeCific foR Hispanic bReaSt Cancer survivORs. the continuAtiOn of work in thIs aRea has ReVolVEd aboUt tHe anaLysis oF LymphoCyte respoNsIVeness IN vitro tO A PuTatiVe suppressive

study will look to see if  there are  diff ere nce sin t hese  factors betwe e n Hi spanics and non-Hispan ics a nd base d  o n tho se diff e re n c esco nd uct  a tr ial t o i ncrease  physicalact iv ity specific fo r Hispanic  br east cancersur vivors .The conti nua tionof wor k  in th is area h as revolv e d about t he  ana lysis of lymphocy t er esponsivenessin vit ro to a  pu tat ive suppre ss ive

study_will look_to see if there_are differences_in_these factors_between_Hispanics and non-Hispanics_and based on_those differences conduct a_trial to increase_physical_activity specific for Hispanic breast cancer survivors. The continuation of work in this area_has_revolved about_the_analysis_of lymphocyte responsiveness in vitro_to a putative suppressive


with new formative data collected at the beginning of the project to enhance the intervention's feeding and physical activity components. Each component in the community-wide interventions will be collaboratively designed with the tribes, and tailored to each community's needs. Trained peer counselors will deliver the family interventions,during a series of 12

with new formative data collected at the beginning of the project to enhance the interposition's eating and physical activity component. Each part in the community - wide interventions will be collaboratively design with the tribes, and tailored to each residential district's motivation. Trained peer advocate will deliver the family intervention, during a series of 12

wihh new formative data coulected at the ywginniig of tge projezt to enhance the interventiln'w feeeing and physical actixity complnents. Eqch romponent in the communibv-wide lnterreitions will be gollaboratieely designed figh the tribes, and tailored to each coimunity'x jeeds. Trained keer botnsemors will deliver the family interbentionv,during a seroes of 12

with new formative data collected at the the to enhance intervention's feeding and in community-wide interventions will collaboratively designed with tribes, and tailored to each community's Trained peer counselors will deliver the family interventions,during a series of 12

with new formative data colleCted at the bEginnIng Of tHe ProjEct tO enhance the intERvenTion's feeding and physicaL actiViTY comPOnEnts. EAch compONeNT In tHe CoMmuNiTY-wIde inTerVentionS will be colLabOrAtively desigNEd With the triBes, And tailored tO eaCh commUnIty'S Needs. traIned pEer couNSelors Will delivEr THe famiLY intervENTiOns,dUring a series of 12

with new formative data c ollected a t the  be gin ni ng o f th e project to e n hanc e the intervention's f eedin ga nd p h ys icalactivit y  c o m pon en ts . E ac h  c ompon ent  in the  community -wi de  interventio n swill be co lla boratively d esi gned w it h t h e tri bes , and  tailo r ed toeach comm un i ty's n e eds. Tr a i ne d pe er counselors wil l  d e liver the fami ly int er v en t i ons ,du ring a ser ie s of1 2

with_new formative_data collected at the_beginning of_the_project to_enhance_the intervention's feeding_and physical activity_components. Each component in_the community-wide interventions_will_be collaboratively designed with the tribes, and tailored to each community's needs. Trained peer_counselors_will deliver_the_family_interventions,during a series of 12


dose-related. Alternatively, these will depend on the individual's underlying motor capabilities, the amount of change in spasticity and strength produced by ITB, and how these changes interact to alter functional performance. Our long term goal is to improve spasticity management in CP by more precise patient selection and dosage adjustment,

dose - related. Alternatively, these will depend on the individual's underlie motor capability, the amount of change in spasticity and strength produce by ITB, and how these changes interact to alter running performance. Our long term finish is to better spasticity management in CP by more precise patient excerpt and dosage adjustment,

dode-related. Alternatively, uhese will depend on the indivjdual's uvderlying motor capabilities, tye amiunt of change in spasgicity anf strengrh pcoduced by ITB, ais how tmzse cgwngev interact to akter functhonal performatcd. Lur long term goal is to improve spwsticitu lanagement in SP bj iore irtcise patient selection and dosags adjusument,

dose-related. Alternatively, these will depend on the motor the amount change in spasticity and these changes interact alter functional performance. long term goal is to improve management in CP by more precise patient selection and dosage adjustment,

dose-related. Alternatively, tHese will dePend oN thE inDiViduAl's uNderlying motor CApabIlities, the amount of chanGe in sPaSTiciTY aNd strEngth prODuCED by iTb, aNd hOw THeSe chaNgeS interaCt to alter fUncTiOnal performaNCe. our long terM goAl is to improvE spAsticiTy ManAGemenT in cP by mOre preCIse patIent selecTiON and doSAge adjuSTMeNt,

dose-related. Alternative ly, thesewilldep end  o n th e in dividual's und e rlyi ng motor capabilities,  theam o unto fchang e in sp a st i c ity  a nd  st re n gt h pro duc ed by I TB, and ho w t he se changes i n te ract to al ter  functionalper forman ce . O u r lon g t erm g oal is to imp rove spas ti c ity ma n agement i nCP b y more precise pa t ie n t selection an d dosa ge ad j u stm ent ,

dose-related._Alternatively, these_will depend on the_individual's underlying_motor_capabilities, the_amount_of change in_spasticity and strength_produced by ITB, and_how these changes_interact_to alter functional performance. Our long term goal is to improve spasticity management in_CP_by more_precise_patient_selection and dosage adjustment,


use of condoms as an effective means for preventing the transmission of the human immunodeficiency virus (HIV) and other sexually transmitted diseases. Universal precaution procedures for health care workers requires wearing, and changing, latex examination gloves. Formed rubber latex items frequently reduce but do not eliminate completely the possibility of transmission of viruses such as HIV

use of condoms as an effective means for preventing the transmittance of the human immunodeficiency virus (HIV) and early sexually transmitted diseases. cosmopolitan precaution procedure for health care workers necessitate wearing, and changing, latex paint interrogation gloves. shape rubber latex item frequently reduce but do not eliminate completely the possibility of transmission of viruses such as HIV

usf of condoms as an effecuive means for prgvwnting the tdansmisskon of the human immunodeficmenct virys (HIV) and other sexuauly translitted duseawws. Universem precamcion lvocedbrxs for health csre workerv requires weasivg, and changing, latex examination glodes. Forkef rubber latex itekf frsquently reduce but do not eliminafe compnetely the poxsibility of transmission lf vlruses such as HIV

use of condoms as an effective means the of the immunodeficiency virus (HIV) Universal procedures for health workers requires wearing, changing, latex examination gloves. Formed rubber items frequently reduce but do not eliminate completely the possibility of transmission of such as HIV

use of condoms as an effective Means for prEventIng The TrAnsmIssiOn of the human imMUnodEficiency virus (HIV) and otHer seXuALly tRAnSmittEd diseaSEs. uNIveRsAl PreCaUTiOn proCedUres for Health care WorKeRs requires weARiNg, and changIng, Latex examinaTioN gloveS. FOrmED rubbEr lAtex iTems frEQuentlY reduce buT dO Not eliMInate coMPLeTely The possibility of tRAnSMission of virusEs such As hiV

use of condoms as an effe ctive mean s for  pr eve nt ingthetransmission o f  the  human immunodeficienc y vir us (HIV )  a nd ot her sex u al l y  tr an sm itt ed di sease s.Univers al precaut ion  p rocedures fo r  h ealth care  wo rkers requir eswearin g,  an d  chan gin g, la tex ex a minati on gloves .F ormedr ubber l a t ex  ite ms frequently red u ce but do not eli minate  c o mp l e tel y t he possibi li ty of transmi s si o n ofv iruses such a s HIV

use_of condoms_as an effective means_for preventing_the_transmission of_the_human immunodeficiency virus_(HIV) and other_sexually transmitted diseases. Universal_precaution procedures for_health_care workers requires wearing, and changing, latex examination gloves. Formed rubber latex items frequently_reduce_but do_not_eliminate_completely the possibility of transmission_of viruses such as HIV


other studies, examination has begun of the structure of the nuclear pore complex, across which REV, TAT, and other viral proteins cross following viral infection. The structure of the pore complex is explored using recombinant DNA techniques. The major nuclear pore glycoprotein np62 has been cloned, sequenced and expressed in cultured cells. The

other studies, examination has begun of the social organization of the nuclear stoma building complex, across which REV, TAT, and other viral proteins cross postdate viral infection. The structure of the pore building complex is explored using recombinant DNA techniques. The major nuclear stoma glycoprotein np62 has been clone, sequenced and expressed in civilized cells. The

otjer studies, examination mas begun of the structnre of fhe nucldar pore complex, across whici REC, TAT, and other viral protekns cross followibg vmral infection. Tis strucbbre or the 'ore complex is explored gsing recombindng BNA techniques. The major nuclear porq glycolrltein np62 has bgen ckjned, sequenced and expressed in cultursd cellv. The

other studies, examination has begun of the the pore complex, which REV, TAT, following infection. The structure the pore complex explored using recombinant DNA techniques. The nuclear pore glycoprotein np62 has been cloned, sequenced and expressed in cultured cells.

other studies, examination haS begun of thE struCtuRe oF tHe nuCleaR pore complex, acROss wHich REV, TAT, and other viraL protEiNS croSS fOllowIng viraL InFECtiOn. thE stRuCTuRe of tHe pOre compLex is exploRed UsIng recombinaNT DnA techniquEs. THe major nucleAr pOre glyCoProTEin np62 Has Been cLoned, sEQuenceD and expreSsED in culTUred celLS. thE

other studies, examinatio n has begu n ofthe  st ru ctur e of  the nuclear p o re c omplex, across which R EV, T AT ,  and ot her v iral pr o te i n s c ro ss  fo ll o wi ng vi ral  infect ion. The s tru ct ure of the p o re  complex i s e xplored usin g r ecombi na ntD NA te chn iques . Them ajor n uclear po re glycop r otein n p 6 2hasbeen cloned, sequ e nc e d and expresse d in c ul t ur e d  ce lls . The

other_studies, examination_has begun of the_structure of_the_nuclear pore_complex,_across which REV,_TAT, and other_viral proteins cross following_viral infection. The_structure_of the pore complex is explored using recombinant DNA techniques. The major nuclear pore_glycoprotein_np62 has_been_cloned,_sequenced and expressed in cultured_cells. The


natural history of HIV infection and its effects on the lung is derived from studies of infected men. Recent reports suggest that important gender differences exist in the type and frequency of complications, particularly in regard to respiratory disease. In both genders in the general population, cigarette smoking represents the major preventable cause of respiratory morbidity and mortality

natural history of HIV infection and its effects on the lung is derived from report of septic men. Recent report hint that important gender difference exist in the character and frequency of complications, particularly in regard to respiratory disease. In both genders in the general population, cigarette smoke represents the major preventable cause of respiratory unwholesomeness and mortality

nahural history of HIV inftction and its efywcts oi the lhng is ddrived from studies of infecved nen. Rtbent reports suggest ghat impogtant geneer vifferences exisv in the type zkd frzqnency of compligations, pardicularly in ragxrb to respiratory disease. In both genqers in tje general popolatipg, cifarette smoking represents the majkr prevtntable cause of rrspiratory morbidity and mlrtapity

natural history of HIV infection and its the is derived studies of infected important differences exist in type and frequency complications, particularly in regard to respiratory In both genders in the general population, cigarette smoking represents the major preventable of respiratory morbidity and mortality

natural history of HIV infectIon and its eFfectS on The LuNg is DeriVed from studies OF infEcted men. Recent reports sUggesT tHAt imPOrTant gEnder diFFeRENceS eXiSt iN tHE tYpe anD frEquency Of complicaTioNs, Particularly IN rEgard to resPirAtory disease. in bOth genDeRs iN The geNerAl popUlatioN, CigareTte smokinG rEPresenTS the majOR PrEvenTable cause of respiRAtORy morbidity and MortalItY

natural history of HIV in fection an d its  ef fec ts  onthelung is derive d  fro m studies of infectedmen.Re c entr ep ortssuggest th a t  im po rt ant  g e nd er di ffe rencesexist in t hety pe and frequ e nc y of compl ica tions, parti cul arly i nreg a rd to  re spira tory d i sease.  In bothge n ders i n  the ge n e ra l po pulation, cigaret t es moking represe nts th em aj o r  pr eve ntable cau se  of r e spirato r ym o r bid i ty and mortal ity

natural_history of_HIV infection and its_effects on_the_lung is_derived_from studies of_infected men. Recent_reports suggest that important_gender differences exist_in_the type and frequency of complications, particularly in regard to respiratory disease. In both_genders_in the_general_population,_cigarette smoking represents the major_preventable cause of respiratory morbidity_and mortality


first human example of a p53 target RE sequence specifically responsible for endogenous gene induction. CANCER-ASSOCIATED P53 MUTANTS. Nearly all cancers have mutant or reduced expression of the p53 tumor suppressor gene. Using yeast-based and human cell systems, we show that functional p53 mutations

first human example of a p53 target RE succession specifically creditworthy for endogenous gene trigger. CANCER - ASSOCIATED P53 MUTANTS. about all cancer have mutant or reduced construction of the p53 tumor suppressor gene. use yeast - based and human cellular telephone system, we show that functional p53 mutations

figst human example of a p53 target RE sequgnxe sperificalmy respovsible for endogenous gene iiducrion. XANCER-ASSOCIATED P53 MUTXNTS. Nearpy all cqncecs have mutant oc reduced exprsdsiou if the p53 tumor suppressos gene. Using yaart-yased and human cell systems, we show that finftional p53 mutajions

first human example of a p53 target specifically for endogenous induction. CANCER-ASSOCIATED P53 mutant reduced expression of p53 tumor suppressor Using yeast-based and human cell systems, show that functional p53 mutations

first human example of a p53 targEt RE sequenCe speCifIcaLlY resPonsIble for endogenOUs geNe induction. CANCER-ASSOCiATED p53 MutANTs. neArly aLl canceRS hAVE muTaNt Or rEdUCeD exprEssIon of thE p53 tumor supPreSsOr gene. Using yEAsT-based and hUmaN cell systems, We sHow thaT fUncTIonal P53 muTatioNs

first human example of ap53 target  RE s equ enc espec ific ally responsib l e fo r endogenous gene indu ction .C ANCE R -A SSOCI ATED P5 3  M U T ANT S.  N ear ly al l can cer s havemutant orred uc ed expressio n  o f the p53tum or suppresso r g ene. U si ngy east- bas ed an d huma n  cellsystems,we show t h at func t i on al p 53 mutations

first_human example_of a p53 target_RE sequence_specifically_responsible for_endogenous_gene induction. CANCER-ASSOCIATED_P53 MUTANTS. Nearly_all cancers have mutant_or reduced expression_of_the p53 tumor suppressor gene. Using yeast-based and human cell systems, we show that_functional_p53 mutations


enediphosphonate), and Guanosine 3',5'-Bis(methylenediphosphonate) G.N. Bennett, G.R. Gough, and P.T. Gilham (1976), Biochemistry 15:4623-4628. Separation of Oligonucleotides, Nucle

enediphosphonate), and Guanosine 3',5'-Bis(methylenediphosphonate) G.N. Bennett, G.R. Gough, and P.T. Gilham (1976), Biochemistry 15:4623 - 4628. Separation of Oligonucleotides, Nucle

enefiphosphonate), and Guanoslne 3',5'-Bis(methylenebuphospionate) F.N. Bennegt, G.R. Gough, and P.T. Gilham (1976), Bmochwmistey 15:4623-4628. Separation of Oligunucleotifes, Nuclw

enediphosphonate), and Guanosine 3',5'-Bis(methylenediphosphonate) G.N. Bennett, G.R. P.T. (1976), Biochemistry Separation of Oligonucleotides,

enediphosphonate), and GuanosIne 3',5'-Bis(methYleneDipHosPhOnatE) G.N. BEnnett, G.R. Gough, aND P.T. GIlham (1976), Biochemistry 15:4623-4628. SeparAtion Of oLigoNUcLeotiDes, NuclE

enediphosphonate), and Gua nosine 3', 5'-Bi s(m eth yl ened ipho sphonate) G.N. Benn ett, G.R. Gough, and P .T. G il h am ( 1 97 6), B iochemi s tr y 15: 46 23 -46 28 .  S epara tio n of Ol igonucleot ide s,  Nucle

enediphosphonate), and_Guanosine 3',5'-Bis(methylenediphosphonate)_G.N. Bennett, G.R. Gough,_and P.T._Gilham_(1976), Biochemistry_15:4623-4628._Separation of Oligonucleotides,_Nucle


(a) are manifestations of males' (vs. females') greater instrumental traits and females' (vs. males') greater expressive traits, (b) arise from the perception that males are generally more competent than females, and (c) arise from the perception that males have greater legitimate authority than females. A number of

(a) are manifestations of males' (vs. females') great implemental traits and females' (vs. male') great expressive traits, (b) arise from the sensing that male are generally more competent than females, and (c) arise from the perception that male have greater legitimate assurance than females. A number of

(a) wre manifestations of maues' (vs. females') yeeater instrhmental graits and females' (vs. males') jrearer ezpressive traits, (b) arire from tje perceptioi that males are generally mors com'event than femalgs, and (c) arive from the pescdpcion that males have greater legitimwte autnogity than femajes. S numgvr of

(a) are manifestations of males' (vs. females') traits females' (vs. greater expressive traits, that are generally more than females, and arise from the perception that males greater legitimate authority than females. A number of

(a) are manifestations of males' (Vs. females') gReateR inStrUmEntaL traIts and females' (vS. MaleS') greater expressive traiTs, (b) arIsE From THe PercePtion thAT mALEs aRe GeNerAlLY mOre coMpeTent thaN females, anD (c) aRiSe from the perCEpTion that maLes Have greater lEgiTimate AuThoRIty thAn fEmaleS. A numbER of

(a) are manifestations of  males' (v s. fe mal es' )grea terinstrumental t r aits  and females' (vs. mal es')gr e ater ex press ive tra i ts , (b)  a ri sefr o mthe p erc eptionthat males  ar egenerally mo r ecompetenttha n females, a nd(c) ar is e f r om th e p ercep tion t h at mal es have g re a ter le g itimate a ut hori ty than females.A  n u mber of

(a)_are manifestations_of males' (vs. females')_greater instrumental_traits_and females'_(vs._males') greater expressive_traits, (b) arise_from the perception that_males are generally_more_competent than females, and (c) arise from the perception that males have greater legitimate_authority_than females._A_number_of


optimal use of the resource by reviewing applications. A detailed chimpanzee brain atlas will be produced during Phase II. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE DESCRIPTION (Applicant's Description-edited) The Dallas County Familial Breast Cancer Registry at UT Southwestern Medical Center

optimal use of the resource by reviewing applications. A detailed chimpanzee mind atlas will be produce during Phase II. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE DESCRIPTION (Applicant's Description - edited) The Dallas County Familial Breast Cancer Registry at UT Southwestern Medical Center

ophimal use of the resourct by reviewing apklucatiois. A defailed cfimpanzee brain atlas will bx priducee during Phase II. PROPUSED COMMVRCIAL APPLICETION: NOT AVAILAUME DESCVNPTIOH (Appnmcant's Descriptlon-edited) Tve Dallas Coundy Fcmilial Breast Cancer Registry at UT Southwrshern Medical Cgnter

optimal use of the resource by reviewing detailed brain atlas be produced during NOT DESCRIPTION (Applicant's Description-edited) Dallas County Familial Cancer Registry at UT Southwestern Medical

optimal use of the resource by Reviewing aPplicAtiOns. a dEtaiLed cHimpanzee brain ATlas Will be produced during PhAse II. pRopOSEd cOmMERCiAL APPLicAtioN: NoT aVaILaBle DeSCRIpTIoN (AppliCant's DescrIptIoN-edited) The DaLLaS County FamIliAl Breast CancEr REgistrY aT UT sOuthwEstErn MeDical CENter

optimal use of the resour ce by revi ewing  ap pli ca tion s. A  detailed chim p anze e brain atlas will beprodu ce d  dur i ng  Phas e II. P R OP O S EDCO MM ERC IA L  A PPLIC ATI ON: NOT  AVAILABLE  DE SC RIPTION (App l ic ant's Desc rip tion-edited)  Th e Dall as  Co u nty F ami lialBreast Cancer  Registry  a t  UT So u thweste r n  M edic al Center

optimal_use of_the resource by reviewing_applications. A_detailed_chimpanzee brain_atlas_will be produced_during Phase II._PROPOSED COMMERCIAL APPLICATION: NOT_AVAILABLE DESCRIPTION (Applicant's_Description-edited)_The Dallas County Familial Breast Cancer Registry at UT Southwestern Medical Center


to use a unique participant recruiting opportunity through a CDC-funded study in the local health department family planning clinic, to enroll a subset of participants from the larger study into a primary qualitative data collection effort to examine neighborhood context and "food activity spaces" as viewed by women of diverse demographic groups (Aim 1). In Aim

to use a unique participant recruiting opportunity through a CDC - funded discipline in the local health department syndicate planning clinic, to enroll a subset of participants from the larger study into a elementary qualitative data collection attempt to examine neighborhood context and " food action spaces " as viewed by womanhood of diverse demographic groups (Aim 1). In bearing

to use a unique participanu recruiting oppottynity vhrough a CDC-fuvded study in the local healvh dwpartnent family planning cuinic, to vnroll a wubstt of participants from tmz larfcr stbdb into a primari qualitativa data collecthov zffort to examine neighborhood contevt and "gold activity spwces" ws vjvwtd by women of diverse demographid groupv (Aim 1). In Aim

to use a unique participant recruiting opportunity CDC-funded in the health department family subset participants from the study into a qualitative data collection effort to examine context and "food activity spaces" as viewed by women of diverse demographic groups 1). In Aim

to use a unique participant reCruiting opPortuNitY thRoUgh a cDC-fUnded study in thE LocaL health department familY planNiNG cliNIc, To enrOll a subSEt OF ParTiCiPanTs FRoM the lArgEr study Into a primaRy qUaLitative data COlLection effOrt To examine neiGhbOrhood CoNteXT and "fOod ActivIty spaCEs" as viEwed by womEn OF diverSE demogrAPHiC groUps (Aim 1). In Aim

to use a unique participa nt recruit ing o ppo rtu ni ty t hrou gh a CDC-funde d  stu dy in the local health  depa rt m entf am ily p lanning cl i n ic,  t oenr ol l  a  subs etof part icipants f rom  t he larger st u dy  into a pr ima ry qualitati vedata c ol lec t ion e ffo rt to  exami n e neig hborhoodco n text a n d "food a ct ivit y spaces" as view e db y women of div erse d em o gr a p hic  gr oups (Aim1) . InA im

to_use a_unique participant recruiting opportunity_through a_CDC-funded_study in_the_local health department_family planning clinic,_to enroll a subset_of participants from_the_larger study into a primary qualitative data collection effort to examine neighborhood context and_"food_activity spaces"_as_viewed_by women of diverse demographic_groups (Aim 1). In Aim