. Unfortunately, EAMs such as Wisepill assume, but cannot verify, actual ingestion of oral medication. In contrast, My/Treatment/Pill (MyTPill), an innovative technology that directly measures ingestion, comprises a digital pill containing a medication and tiny radio emitter in a gelatin capsule. When the digital

. Unfortunately, EAMs such as Wisepill assume, but cannot verify, actual ingestion of oral medication. In contrast, My / Treatment / Pill (MyTPill), an advanced engineering that immediately measures ingestion, comprises a digital pill hold a medication and tiny radio receiver emitter in a gelatin capsule. When the digital

. Unvortunately, EAMs such as Wisepill assumg, vut cainot vedify, actjal ingestion of oral medicavion. In cintrast, My/Treatment/Pilu (MyTPill), an innocatite technology thef direcbjy mswsurzs ingestion, comkrises a dightal pill contdiviug a medication and tiny radio emittqr in a gflatin capsule. Whem the digital

. Unfortunately, EAMs such as Wisepill assume, verify, ingestion of medication. In contrast, that measures ingestion, comprises digital pill containing medication and tiny radio emitter in gelatin capsule. When the digital

. Unfortunately, EAMs such as WiSepill assuMe, but CanNot VeRify, ActuAl ingestion of oRAl meDication. In contrast, My/TrEatmeNt/pIll (MYtPIll), an InnovatIVe TEChnOlOgY thAt DIrEctly MeaSures inGestion, comPriSeS a digital pilL CoNtaining a mEdiCation and tinY raDio emiTtEr iN A gelaTin CapsuLe. When THe digiTal

. Unfortunately, EAMs such  as Wisepi ll as sum e,bu t ca nnot  verify, actua l  ing estion of oral medicat ion.In cont r as t, My /Treatm e nt / P ill  ( My TPi ll ) ,an in nov ative t echnologytha tdirectly mea s ur es ingesti on,  comprises a  di gitalpi llc ontai nin g a m edicat i on and  tiny rad io emitte r  in a g e l at in c apsule. When thed ig i tal

. Unfortunately,_EAMs such_as Wisepill assume, but_cannot verify,_actual_ingestion of_oral_medication. In contrast,_My/Treatment/Pill (MyTPill), an_innovative technology that directly_measures ingestion, comprises_a_digital pill containing a medication and tiny radio emitter in a gelatin capsule. When_the_digital


larger de-identified database of prescriptions covering the entire Washington, D.C. metropolitan area. With this database we will seek to answer many questions about prescribing patterns, including, but not limited to: the degree to which drug interacting pairs written by different providers from different offices would not be seen as an interaction by the provider

larger de - identified database of prescriptions covering the integral Washington, D.C. metropolitan sphere. With this database we will try to answer many question about prescribing practice, including, but not limited to: the academic degree to which drug interacting pairs written by unlike supplier from different office would not be seen as an interaction by the provider

lagger de-identified databare of prescriptnins cotering fhe entife Washington, D.C. metropolitai arwa. Wiuk this database we wkll seek no answer manb questions abouv prescrlying lwttexnw, including, buj not limiteg to: the degrea go which drug interacting pairs writtqn by dofverent providets frpi dirferent offices would not be seen zs an iiteraction by tne provider

larger de-identified database of prescriptions covering the D.C. area. With database we will about patterns, including, but limited to: the to which drug interacting pairs written different providers from different offices would not be seen as an interaction by provider

larger de-identified databasE of prescriPtionS coVerInG the EntiRe Washington, D.C. MEtroPolitan area. With this datAbase We WIll sEEk To ansWer many QUeSTIonS aBoUt pReSCrIbing PatTerns, inCluding, but Not LiMited to: the deGReE to which drUg iNteracting paIrs WritteN bY diFFerenT prOvideRs from DIffereNt offices WoULd not bE Seen as aN INtEracTion by the provider

larger de-identified data base of pr escri pti ons  c over ingthe entire Was h ingt on, D.C. metropolitanarea.  W i th t h is  data base we wi l l  se ek  t o a ns w er  many  qu estions  about pre scr ib ing patterns ,  i ncluding,but  not limited  to : thede gre e  to w hic h dru g inte r acting  pairs wr it t en byd ifferen t pr ovid ers from differen t  o f fices would no t be s ee n  a s anint eraction b ythe p r ovider

larger_de-identified database_of prescriptions covering the_entire Washington,_D.C._metropolitan area._With_this database we_will seek to_answer many questions about_prescribing patterns, including,_but_not limited to: the degree to which drug interacting pairs written by different providers_from_different offices_would_not_be seen as an interaction_by the provider


modulating pathway-specific ERK activation should facilitate new GPCR drug discovery efforts for treating mental disorders. We propose four aims for the project. Aim 1. Develop G-protein-dependent ERK2 activation pathway assay. We plan to utilize phosphorylated ERK2 interaction with imp7 as signal readout for assay development. We plan to validate

modulating pathway - specific ERK activation should facilitate newfangled GPCR drug discovery feat for treating mental disorders. We aim four aims for the project. bearing 1. Develop G - protein - subject ERK2 activation nerve pathway try. We plan to utilize phosphorylated ERK2 interaction with imp7 as signal readout for assay exploitation. We plan to validate

mofulating pathway-specific ERK activation should faciljtate ned GPCR drug discovery effortd dor teeating mental disordefs. We proiose four aimw for the pckject. Ali 1. Dsyelop J-protein-dependekt ERK2 actieation pathway arscy. We plan to utilize phosphorylated ERK2 inyegaction with iip7 ax sighal readout for assay development. Se plan to validate

modulating pathway-specific ERK activation should facilitate new discovery for treating disorders. We propose Aim Develop G-protein-dependent ERK2 pathway assay. We to utilize phosphorylated ERK2 interaction with as signal readout for assay development. We plan to validate

modulating pathway-specific eRK activatIon shOulD faCiLitaTe neW GPCR drug discoVEry eFforts for treating mentaL disoRdERs. We PRoPose fOur aims FOr THE prOjEcT. AiM 1. DEVeLop G-pRotEin-depeNdent ERK2 acTivAtIon pathway asSAy. we plan to utIliZe phosphorylAteD ERK2 inTeRacTIon wiTh iMp7 as sIgnal rEAdout fOr assay deVeLOpment. wE plan to VALiDate

modulating pathway-specif ic ERK act ivati onsho ul d fa cili tate new GPCRd rugdiscovery efforts fortreat in g  men t al  diso rders.W ep r opo se  f our  a i ms  forthe  projec t. Aim 1.Dev el op G-protein - de pendent ER K2activation p ath way as sa y.W e pla n t o uti lize p h osphor ylated ER K2 intera c tion wi t h  i mp7as signal readout fo r  assay develop ment.We pl a n  to  va lidate

modulating_pathway-specific ERK_activation should facilitate new_GPCR drug_discovery_efforts for_treating_mental disorders. We_propose four aims_for the project. Aim_1. Develop G-protein-dependent_ERK2_activation pathway assay. We plan to utilize phosphorylated ERK2 interaction with imp7 as signal_readout_for assay_development._We_plan to validate


ates the mechanisms by which cells die if they do not receive the correct developmental information. These incorrectly informed cells resemble cancer cells, and the observation that they die may protect the organism from several common diseases. Understanding the mechanisms of this process may lead to new therapeutic interventions for cancer, and may also be relevant for treatment of neurodegenerative

ates the mechanisms by which cells die if they do not receive the right developmental data. These falsely informed cells resemble cancer cells, and the observation that they fail may protect the organism from several common diseases. sympathize the mechanisms of this process may moderate to new therapeutic intervention for cancer, and may also be relevant for treatment of neurodegenerative

ated the mechanisms by whicm cells die if tkwy do iot recsive the correct developmental inforlarion. Ukese incorrectly infurmed celps resemvle rancer cells, and the obscxvatikk thac vhey die may prptect the mrganism from vexexal common diseases. Understanding thq mechamidms of this prjcesx may lead to new therapeutic interventjons fog cancer, and may slso be relevant for treatlent of neurodegeneratlve

ates the mechanisms by which cells die do receive the developmental information. These cells, the observation that die may protect organism from several common diseases. Understanding mechanisms of this process may lead to new therapeutic interventions for cancer, and also be relevant for treatment of neurodegenerative

ates the mechanisms by which cElls die if tHey do Not RecEiVe thE corRect developmenTAl inFormation. These incorrecTly inFoRMed cELlS reseMble canCEr CELls, AnD tHe oBsERvAtion ThaT they diE may protecT thE oRganism from sEVeRal common dIseAses. UnderstaNdiNg the mEcHanISms of ThiS procEss may LEad to nEw therapeUtIC interVEntions FOR cAnceR, and may also be releVAnT For treatment of NeurodEgENeRATivE

ates the mechanisms by whi ch cells d ie if  th eydo  not  rec eive the corre c t de velopmental informatio n. Th es e  inc o rr ectly  inform e dc e lls  r es emb le ca ncercel ls, and  the obser vat io n that theyd ie  may prote ctthe organism  fr om sev er alc ommon  di sease s. Und e rstand ing the m ec h anisms of this p ro cess  may lead to newt he r apeutic interv ention sf or c anc er,  and may a ls o ber elevant fo r t rea t ment of neuro degenerativ e

ates the_mechanisms by_which cells die if_they do_not_receive the_correct_developmental information. These_incorrectly informed cells_resemble cancer cells, and_the observation that_they_die may protect the organism from several common diseases. Understanding the mechanisms of this_process_may lead_to_new_therapeutic interventions for cancer, and_may also be relevant for_treatment of_neurodegenerative


is found in 90% of all olfactory receptor neurons, the other receptor (that responds to acetophenone) is present in just 5% of cells and these do not overlap with the cells expressing the octanal receptor. We are therefore examining the mechanisms that account for this completely unexpected gene expression pattern and believe that they will

is found in 90% of all olfactory receptor neurons, the other sense organ (that respond to acetophenone) is present in just 5% of cells and these do not overlap with the cell express the octanal receptor. We are therefore examine the mechanism that account for this wholly unexpected gene formula pattern and think that they will

is found in 90% of all olfactury receptor neorins, thx other receptof (that responds to acetophenlnw) is kgesent in just 5% of ceuls and tjese do bot icerlap witi the cells exlvessiug the octanal rgceptor. We ase therefore efaoiuing the mechanisms that account for this cpmoletely unexpested dene vxkression pattern and believe that they wpll

is found in 90% of all olfactory the receptor (that to acetophenone) is cells these do not with the cells the octanal receptor. We are therefore the mechanisms that account for this completely unexpected gene expression pattern and believe they will

is found in 90% of all olfactory reCeptor neurOns, thE otHer ReCeptOr (thAt responds to acETophEnone) is present in just 5% of Cells AnD ThesE Do Not ovErlap wiTH tHE CelLs ExPreSsINg The ocTanAl recepTor. We are thEreFoRe examining tHE mEchanisms tHat Account for thIs cOmpletElY unEXpectEd gEne exPressiON patteRn and beliEvE That thEY will

is found in 90% of all ol factory re cepto r n eur on s, t he o ther receptor( that  responds to acetophen one)is pres e nt  in j ust 5%o fc e lls  a nd  th es e  d o not  ov erlap w ith the ce lls  e xpressing th e  o ctanal rec ept or. We are t her eforeex ami n ing t hemecha nismst hat ac count for  t h is com p letelyu n ex pect ed gene expressio n  p a ttern and beli eve th at th e y  wi ll

is_found in_90% of all olfactory_receptor neurons,_the_other receptor_(that_responds to acetophenone)_is present in_just 5% of cells_and these do_not_overlap with the cells expressing the octanal receptor. We are therefore examining the mechanisms_that_account for_this_completely_unexpected gene expression pattern and_believe that they will


-induced spinal glia activation in the modulation of visceral sensitivity and determine the molecular pathways engaged in the initiation and maintenance of visceral hyperalgesia. Our first specific aim is to assess the temporal profile of spinal glia activation during and after chronic stress and to test the ability of inhibitors specific of glia to reduce stress

-induced spinal glia activation in the modulation of visceral sensitivity and settle the molecular nerve pathway engaged in the initiation and maintenance of intuitive hyperalgesia. Our first specific aim is to assess the worldly visibility of spinal glia activation during and after chronic stress and to test the ability of inhibitors specific of neuroglia to reduce stress

-indkced spinal glia activatlon in the modulcrion oh viscedal sensktivity and determine the mopexular pathways engaged in tfe initianion and nainuenance of viscerem hyperalgesiz. Our hirst specific sim is to dssess the tem[ofap profile of spinal glia activation during ajd after chronyc surefs ahd to test the ability of inhibitods specpfic of glia to rrduce stress

-induced spinal glia activation in the modulation sensitivity determine the pathways engaged in visceral Our first specific is to assess temporal profile of spinal glia activation and after chronic stress and to test the ability of inhibitors specific of to reduce stress

-induced spinal glia activatiOn in the modUlatiOn oF viScEral SensItivity and deteRMine The molecular pathways enGaged In THe inITiAtion And mainTEnANCe oF vIsCerAl HYpEralgEsiA. Our firSt specific Aim Is To assess the tEMpOral profilE of Spinal glia acTivAtion dUrIng ANd aftEr cHroniC stresS And to tEst the abiLiTY of inhIBitors sPECiFic oF glia to reduce streSS

-induced spinal glia activ ation in t he mo dul ati on  ofvisc eral sensitivi t y an d determine the molecu lar p at h ways en gaged  in the in i t iat io nand  m a in tenan ceof visc eral hyper alg es ia. Our firs t  s pecific ai m i s to assessthe  tempo ra l p r ofile  of  spin al gli a  activ ation dur in g  and a f ter chr o n ic  str ess and to test t h ea bility of inhi bitors  s p ec i f icofglia to re du ce st r ess

-induced spinal_glia activation_in the modulation of_visceral sensitivity_and_determine the_molecular_pathways engaged in_the initiation and_maintenance of visceral hyperalgesia._Our first specific_aim_is to assess the temporal profile of spinal glia activation during and after chronic_stress_and to_test_the_ability of inhibitors specific of_glia to reduce stress


ses. Biochemical comparisons of the parameters of pyridine-linked dehydrogenases suggest a common evolutionary origin of these enzymes. In Drosophila melangaster there appears to be two forms of NADP-isocitrate dehyrogenase and there may be two genes controlling the activity of this enzyme. A molecular characterization

ses. Biochemical comparisons of the parameters of pyridine - linked dehydrogenases indicate a coarse evolutionary origin of these enzymes. In Drosophila melangaster there appears to be two kind of NADP - isocitrate dehyrogenase and there may be two genes controlling the activeness of this enzyme. A molecular portrayal

ses. Biochemical comparisons of the parametgrw of pbridine-minked ddhydrogenases suggest a commln evolytionary origin of there enzymed. In Drowophmla melangaster vgere apizars fl be vwo forms of NACP-isocitrade dehyrogenasa xnb there may be two genes controlling the acyigity of this egzymt. A molsbuoar characterization

ses. Biochemical comparisons of the parameters of suggest common evolutionary of these enzymes. to two forms of dehyrogenase and there be two genes controlling the activity this enzyme. A molecular characterization

ses. Biochemical comparisons Of the paramEters Of pYriDiNe-liNked Dehydrogenases SUggeSt a common evolutionary oRigin Of THese ENzYmes. IN DrosopHIlA MElaNgAsTer ThERe AppeaRs tO be two fOrms of NADP-IsoCiTrate dehyrogENaSe and there May Be two genes coNtrOlling ThE acTIvity Of tHis enZyme. A mOLeculaR characteRiZAtion

ses. Biochemical compariso ns of theparam ete rsof  pyr idin e-linked dehyd r ogen ases suggest a commonevolu ti o nary or iginof thes e  e n z yme s.  I n D ro s op hilamel angaste r there ap pea rs  to be two f o rm s of NADP- iso citrate dehy rog enasean d t h ere m aybe tw o gene s  contr olling th ea ctivit y  of thi s en zyme . A molecular cha r ac t erization

ses. Biochemical_comparisons of_the parameters of pyridine-linked_dehydrogenases suggest_a_common evolutionary_origin_of these enzymes._In Drosophila melangaster_there appears to be_two forms of_NADP-isocitrate_dehyrogenase and there may be two genes controlling the activity of this enzyme. A_molecular_characterization


pursue research careers that require training in Cellular and Molecular Biology;the superb record of our past trainees supports this expectation. Facilities are located in a complex of adjacent buildings. Multi-user facilities include cell sorting, biological imaging including cryoelectron microscopy, NMR and mass spectrometry, monoclonal antibody production, high throughput DMA sequencing,

pursue research careers that require education in Cellular and Molecular Biology;the superb phonograph record of our past trainees supports this anticipation. adeptness are located in a complex of adjacent building. Multi - drug user facilities admit cellular telephone sorting, biological imaging admit cryoelectron microscopy, NMR and mass spectrometry, monoclonal antibody production, eminent throughput DMA sequencing,

pugsue research careers thxt require traiuung in Cellumar and Oolecular Biology;the superb cecoed of our past trainees supoorts thid expectqtioi. Facilities are located in a dlmplzx of adjacent boildings. Muldi-user facilither nnclude cell sorting, biological imagyng inckufing cryoelectton mpcwosckiy, NMR and mass spectrometry, mohoclonan antibody prpduction, high throughput DLA sfquencing,

pursue research careers that require training in Molecular superb record our past trainees located a complex of buildings. Multi-user facilities cell sorting, biological imaging including cryoelectron NMR and mass spectrometry, monoclonal antibody production, high throughput DMA sequencing,

pursue research careers that Require traIning In CEllUlAr anD MolEcular Biology;tHE supErb record of our past traiNees sUpPOrts THiS expeCtation. fAcILItiEs ArE loCaTEd In a coMplEx of adjAcent buildIngS. MUlti-user faciLItIes include CelL sorting, biolOgiCal imaGiNg iNCludiNg cRyoelEctron MIcroscOpy, NMR and MaSS spectROmetry, mONOcLonaL antibody productiON, hIGh throughput DMa sequeNcINg,

pursue research careers t hat requir e tra ini ngin  Cel lula r and Molecula r  Bio logy;the superb record  of o ur past tr ainee s suppo r ts t his  e xp ect at i on . Fac ili ties ar e locatedinacomplex of a d ja cent build ing s. Multi-use r f acilit ie s i n clude  ce ll so rting, biolog ical imag in g  inclu d ing cry o e le ctro n microscopy, NMR an d  mass spectrom etry,mo n oc l o nal  an tibody pro du ction ,  high t h ro u g h put DMA sequencin g,

pursue_research careers_that require training in_Cellular and_Molecular_Biology;the superb_record_of our past_trainees supports this_expectation. Facilities are located_in a complex_of_adjacent buildings. Multi-user facilities include cell sorting, biological imaging including cryoelectron microscopy, NMR and_mass_spectrometry, monoclonal_antibody_production,_high throughput DMA sequencing,


. The need to address substance use within the women offender population has generated interest in Recovery Management Checkups (RMCs), an evidence-based approach to promoting long-term recovery that provides clients with periodic monitoring, feedback, and linkage with treatment as needed. The Recovery Management Checkups for Women Offenders (RMC-

. The need to address substance use within the charwoman wrongdoer population has generated interest in Recovery Management Checkups (RMCs), an evidence - free-base approach path to promoting long - terminus convalescence that provides clients with periodic monitoring, feedback, and linkage with treatment as needed. The Recovery Management Checkups for Women Offenders (RMC-

. Thf need to address substakce use within tkw womei offenser popuuation has generated interesv in Recocery Management Checkuos (RMCs), aj evidenxe-bawwd approaci to promoting pong-cecm recovery thaj provides cnients with pesiudnc monitoring, feedback, and linkage wyth trestlent as needed. The Wecobvrn Management Checkups for Women Kffendegs (RMC-

. The need to address substance use women population has interest in Recovery approach promoting long-term recovery provides clients with monitoring, feedback, and linkage with treatment needed. The Recovery Management Checkups for Women Offenders (RMC-

. The need to address substance Use within tHe womEn oFfeNdEr poPulaTion has generatED intErest in Recovery ManagemEnt ChEcKUps (Rmcs), An eviDence-baSEd APProAcH tO prOmOTiNg lonG-teRm recovEry that proVidEs Clients with pERiOdic monitoRinG, feedback, and LinKage wiTh TreATment As nEeded. the RecOVery MaNagement CHeCKups foR women OfFENdErs (RmC-

. The need to address subs tance usewithi n t hewo menoffe nder populatio n  has  generated interest in  Reco ve r y Ma n ag ement  Checku p s( R MCs ),  a n e vi d en ce-ba sed  approa ch to prom oti ng  long-term r e co very thatpro vides client s w ith pe ri odi c  moni tor ing,feedba c k, and  linkagewi t h trea t ment as n ee ded.  The Recovery Man a ge m ent Checkups f or Wom en Of f e nde rs(RMC-

. The_need to_address substance use within_the women_offender_population has_generated_interest in Recovery_Management Checkups (RMCs),_an evidence-based approach to_promoting long-term recovery_that_provides clients with periodic monitoring, feedback, and linkage with treatment as needed. The Recovery_Management_Checkups for_Women_Offenders_(RMC-


, which produce effector cytokines and migrate through the tissues, and central memory T cells that do not produce effector cytokines and migrate through lymph nodes. C57BL/6 mice infected with Leishmania major are immune to rechallenge after they resolve their infections, but this immunity was thought to be dependent upon residual parasites. Now

, which produce effector cytokines and migrate through the tissues, and cardinal memory metric ton cells that do not produce effecter cytokines and migrate through lymph node. C57BL/6 mice infected with Leishmania major are immune to rechallenge after they dissolve their infections, but this immunity was think to be dependent upon residual parasites. immediately

, whlch produce effector cytukines and migrcre thrmugh tge tissuds, and central memory T celld rhat eo not produce effectof cytokinvs and mitratt through lymph nodes. C57BL/6 mice jkfectzd with Leishmanla major ara immune to rewhxlpenge after they resolve their infestions, nuh this immuniti was ehoufht to be dependent upon residual larasitts. Now

, which produce effector cytokines and migrate tissues, central memory cells that do migrate lymph nodes. C57BL/6 infected with Leishmania are immune to rechallenge after they their infections, but this immunity was thought to be dependent upon residual parasites.

, which produce effector cytokInes and migRate tHroUgh ThE tisSues, And central memoRY T ceLls that do not produce effEctor CyTOkinES aNd migRate thrOUgH LYmpH nOdEs. C57bL/6 MIcE infeCteD with LeIshmania maJor ArE immune to recHAlLenge after TheY resolve theiR inFectioNs, But THis imMunIty waS thougHT to be dEpendent uPoN ResiduAL parasiTES. NOw

, which produce effector c ytokines a nd mi gra teth roug h th e tissues, and cent ral memory T cells tha t dono t  pro d uc e eff ector c y to k i nes  a nd  mi gr a te  thro ugh  lymphnodes. C57 BL/ 6mice infecte d  w ith Leishm ani a major areimm une to  r ech a lleng e a fterthey r e solvetheir inf ec t ions,b ut this i mm unit y was thought tob ed ependent uponresidu al pa r a sit es.  Now

, which_produce effector_cytokines and migrate through_the tissues,_and_central memory_T_cells that do_not produce effector_cytokines and migrate through_lymph nodes. C57BL/6_mice_infected with Leishmania major are immune to rechallenge after they resolve their infections, but_this_immunity was_thought_to_be dependent upon residual parasites._Now


ation, fluoresence, and orthograde as well as retrograde axonal tracing techniques; 2) the experimental elimination of these systems with lesion or neurotoxins to assess their contribution to the behavior; and 3) the administration of psychoactive drugs such as the stimulants and neuroleptics before and after the lesions to assess

ation, fluoresence, and orthograde as well as retrograde axonal tracing techniques; 2) the experimental elimination of these systems with wound or neurotoxin to tax their contribution to the behavior; and 3) the presidency of psychoactive drug such as the stimulants and neuroleptics before and after the lesions to measure

atiln, fluoresence, and orthonrade as well as retrogcade axknal trazing techniques; 2) the experimxntao elinination of these systdms with pesion oe nenrotoxins to assxas theiv confvibutnoi to the behavipr; and 3) tha administratimn oy psychoactive drugs such as the stiiulants ajd neurolepticf begjre znd after the lesions to assess

ation, fluoresence, and orthograde as well as tracing 2) the elimination of these to their contribution to behavior; and 3) administration of psychoactive drugs such as stimulants and neuroleptics before and after the lesions to assess

ation, fluoresence, and orthogRade as well As retRogRadE aXonaL traCing techniques; 2) THe exPerimental elimination oF thesE sYStemS WiTh lesIon or neURoTOXinS tO aSseSs THeIr conTriBution tO the behaviOr; aNd 3) The administrATiOn of psychoActIve drugs such As tHe stimUlAntS And neUroLeptiCs befoRE and afTer the lesIoNS to assESs

ation, fluoresence, and or thograde a s wel l a s r et rogr adeaxonal tracing tech niques; 2) the experim ental  e l imin a ti on of  theses ys t e mswi th  le si o nor ne uro toxinsto assessthe ir  contributio n  t o the beha vio r; and 3) th e a dminis tr ati o n ofpsy choac tive d r ugs su ch as the  s t imulan t s and n e u ro lept ics before and af t er the lesions to  asses s

ation, fluoresence,_and orthograde_as well as retrograde_axonal tracing_techniques;_2) the_experimental_elimination of these_systems with lesion_or neurotoxins to assess_their contribution to_the_behavior; and 3) the administration of psychoactive drugs such as the stimulants and neuroleptics_before_and after_the_lesions_to assess


coli also required for antitermination of the rRNA operons interact with the RNA, and can it be identified by crosslinking? Homologous recombination is a critically important process responsible for repairing double-strand DNA breaks and exchanging genetic material between homologous chromosomes in cells undergoing meiosis. Our goal is to understand the mechanisms of homologous

coli also required for antitermination of the rRNA operons interact with the RNA, and can it be identified by crosslinking? Homologous recombination is a critically authoritative procedure responsible for repairing double - fibril DNA breaks and exchange genic material between homologous chromosome in cell undergoing litotes. Our goal is to understand the mechanisms of homologous

copi also required for antltermination of jhw rRNA operohs interxct with the RNA, and can it ue ieentidied by crosslinking? Humologous recombibatiib is a crivjcally liporfwnt 'ricess responsinle for repdiring double-sdrxnb DNA breaks and exchanging genetic iateriak hetween homolodous shrojosomes in cells undergoing meiosia. Our gmal is to undrrstand the mechanisms of jomopogous

coli also required for antitermination of the interact the RNA, can it be is critically important process for repairing double-strand breaks and exchanging genetic material between chromosomes in cells undergoing meiosis. Our goal is to understand the mechanisms of

coli also required for antiteRmination oF the rrNA OpeRoNs inTeraCt with the RNA, anD Can iT be identified by crossliNking? hoMOlogOUs RecomBinatioN Is A CRitIcAlLy iMpORtAnt prOceSs respoNsible for rEpaIrIng double-strANd dNA breaks aNd eXchanging genEtiC materIaL beTWeen hOmoLogouS chromOSomes iN cells undErGOing meIOsis. Our GOAl Is to Understand the mechANiSMs of homologous

coli also required for an titerminat ion o f t herR NA o pero ns interact wi t h th e RNA, and can it be i denti fi e d by cr ossli nking?H om o l ogo us  r eco mb i na tionisa criti cally impo rta nt  process res p on sible forrep airing doubl e-s trandDN A b r eaksand  exch anging geneti c materia lb etween homolog o u schro mosomes in cellsu nd e rgoing meiosis . Ourgo a li s  to  un derstand t he  mech a nisms o f  h o m o log o us

coli_also required_for antitermination of the_rRNA operons_interact_with the_RNA,_and can it_be identified by_crosslinking? Homologous recombination is_a critically important_process_responsible for repairing double-strand DNA breaks and exchanging genetic material between homologous chromosomes in_cells_undergoing meiosis._Our_goal_is to understand the mechanisms_of homologous


. However, such studies in mammals are expensive, time consuming and risky. In contrast, the nematode C. elegans provides an economical system in which to rapidly determine their physiological relevance. To this end, we discovered a truncated isoform of the insulin receptor in the worm, termed DAF-2B, that is directly

. However, such studies in mammals are expensive, time consuming and hazardous. In line, the nematode C. elegans provides an economical arrangement in which to quickly determine their physiological relevance. To this end, we fall upon a truncated isoform of the insulin receptor in the worm, term DAF-2B, that is immediately

. Hoaever, such studies in maomals are expensive, tike conauming avd risky. In contrast, the nemetodw C. eoegans provides an ecovomical sjstem in qhici to rapidly detxdmine tmzir pgnsiolmjical relevance. To this etd, we discoverad a truncated isoform of the insulin rqceptor ij the worm, teried CWF-2B, fhat is directly

. However, such studies in mammals are consuming risky. In the nematode C. in to rapidly determine physiological relevance. To end, we discovered a truncated isoform the insulin receptor in the worm, termed DAF-2B, that is directly

. However, such studies in mammaLs are expenSive, tIme ConSuMing And rIsky. In contrast, THe neMatode C. elegans provides An ecoNoMIcal SYsTem in Which to RApIDLy dEtErMinE tHEiR physIolOgical rElevance. To ThiS eNd, we discoverED a Truncated iSofOrm of the insuLin ReceptOr In tHE worm, TerMed DAf-2B, that IS direcTly

. However, such studies in  mammals a re ex pen siv e,  tim e co nsuming and ri s ky.In contrast, the nemat ode C .e lega n sprovi des ane co n o mic al  s yst em in  whic h t o rapid ly determi neth eir physiolo g ic al relevan ce.  To this end , w e disc ov ere d  a tr unc atedisofor m  of th e insulin  r e ceptor in thew o rm , te rmed DAF-2B, that is directly

. However,_such studies_in mammals are expensive,_time consuming_and_risky. In_contrast,_the nematode C._elegans provides an_economical system in which_to rapidly determine_their_physiological relevance. To this end, we discovered a truncated isoform of the insulin receptor_in_the worm,_termed_DAF-2B,_that is directly


basis of Tat cross-talk with Rho GTPase and its impact on neuronal cell integrity. The University of New Mexico Health Sciences Center (UNM HSC) has developed an innovative and widely applicable model to provide treatment for patients with chronic, common and complex diseases who do not have direct access to specialty healthcare providers. This

basis of Tat cross - talk with Rho GTPase and its impact on neural cellular telephone integrity. The University of New Mexico Health Sciences Center (UNM HSC) has develop an innovative and widely applicable mannequin to provide treatment for affected role with chronic, coarse and complex diseases who do not suffer direct access to specialization healthcare providers. This

badis of Tat cross-talk witm Rho GTPase and its im'act on neuronau cell integrity. The Universmty if Neq Mexico Health Sciencds Center (UNM HSC) has eeveloped eh innovative zkd wibeoy applicable kodel to psovide treatmett flr patients with chronic, common and complec fiseases who dj nou hwve sprtct access to specialty healthcars provivers. This

basis of Tat cross-talk with Rho GTPase impact neuronal cell The University of (UNM has developed an and widely applicable to provide treatment for patients with common and complex diseases who do not have direct access to specialty healthcare This

basis of Tat cross-talk with RhO GTPase and Its imPacT on NeUronAl ceLl integrity. The uNiveRsity of New Mexico Health scienCeS centER (UnM HSC) Has deveLOpED An iNnOvAtiVe ANd WidelY apPlicablE model to prOviDe Treatment for PAtIents with cHroNic, common and ComPlex diSeAseS Who do Not Have dIrect aCCess to Specialty HeALthcarE ProvideRS. thIs

basis of Tat cross-talk w ith Rho GT Paseand  it simpa ct o n neuronal cel l  int egrity. The University  of N ew Mexi c oHealt h Scien c es C ent er  ( UNM  H S C)  hasdev elopedan innovat ive  a nd widely ap p li cable mode l t o provide tr eat ment f or  pa t ients  wi th ch ronic, common  and comp le x  disea s es whod o  n ot h ave direct access to specialty heal thcare  p r ov i d ers . T his

basis_of Tat_cross-talk with Rho GTPase_and its_impact_on neuronal_cell_integrity. The University_of New Mexico_Health Sciences Center (UNM_HSC) has developed_an_innovative and widely applicable model to provide treatment for patients with chronic, common and_complex_diseases who_do_not_have direct access to specialty_healthcare providers. This


and 36 months. The instrument measuring the quality of work and its human outcomes will be used across all demonstrations. It will allow analyses to be made over time of the relationship between the quality of the working environment and a variety of outcomes. This proposal seeks funding for a portion of the research and evaluation component of this Project,

and 36 months. The instrument measuring the quality of workplace and its human result will be used across all demonstrations. It will allow analysis to be made over time of the kinship between the quality of the working environment and a variety of result. This proposal seeks financing for a portion of the research and evaluation part of this Project,

anf 36 months. The instrument measuring the quality of wodk and igs human outcomes will be usxd axross all demonstrations. It will alllw analywes uo be made over tmje of tmz relzbionski' between the qoality of tha working envisovmznt and a variety of outcomes. This pwoposal sfeks funding fjr a [ortjon of the research and evaluation compontnt of this Projecy,

and 36 months. The instrument measuring the work its human will be used allow to be made time of the between the quality of the working and a variety of outcomes. This proposal seeks funding for a portion of research and evaluation component of this Project,

and 36 months. The instrument meaSuring the qUalitY of WorK aNd itS humAn outcomes will BE useD across all demonstratioNs. It wIlL AlloW AnAlyseS to be maDE oVER tiMe Of The ReLAtIonshIp bEtween tHe quality oF thE wOrking enviroNMeNt and a variEty Of outcomes. ThIs pRoposaL sEekS FundiNg fOr a poRtion oF The resEarch and eVaLUation COmponenT OF tHis PRoject,

and 36 months. The instru ment measu ringthe  qu al ityof w ork and its hu m an o utcomes will be used a cross  a l l de m on strat ions. I t  w i l l a ll ow  an al y se s tobemade ov er time of  th erelationship be tween thequa lity of thewor king e nv iro n mentand  a va rietyo f outc omes. Thi sp roposa l  seeksf u nd ingfor a portion oft he research and e valuat io n  c o m pon ent  of this P ro ject,

and_36 months._The instrument measuring the_quality of_work_and its_human_outcomes will be_used across all_demonstrations. It will allow_analyses to be_made_over time of the relationship between the quality of the working environment and a_variety_of outcomes._This_proposal_seeks funding for a portion_of the research and evaluation_component of_this Project,


engraft in the liver and establish a vascular supply. The greater the functional islet mass engrafted, the lower the risk of post-operative diabetes. It has been estimated that more than half of the islet mass may be lost in the early post-transplant period in islet transplant recipients. Beta cell

engraft in the liver and establish a vascular supply. The greater the functional isle mass graft, the lower the risk of post - surgical diabetes. It has been calculate that more than half of the islet batch may be lost in the early post - transplant period in isle graft recipients. Beta cell

enhraft in the liver and ertablish a vascolqr sup'ly. The greater the functional islet mass eigradted, uke lower the risk of post-operwtive diqbetts. It has been esvjmated bkat mkve thcn half of the ixlet mass kay be lost in tfe early post-transplant period in islqt tranxppant recipientf. Beua sell

engraft in the liver and establish a The the functional mass engrafted, the diabetes. has been estimated more than half the islet mass may be lost the early post-transplant period in islet transplant recipients. Beta cell

engraft in the liver and estabLish a vascuLar suPplY. ThE gReatEr thE functional islET masS engrafted, the lower the rIsk of PoST-opeRAtIve diAbetes. IT HaS BEen EsTiMatEd THaT more ThaN half of The islet maSs mAy Be lost in the eARlY post-transPlaNt period in isLet TranspLaNt rECipieNts. beta cEll

engraft in the liver andestablisha vas cul arsu pply . Th e greater thef unct ional islet mass engra fted,  t h e lo w er  therisk of po s t -op er at ive  d i ab etes.  It  has be en estimat edth at more than ha lf of theisl et mass maybelost i nthe early  po st-tr anspla n t peri od in isl et transp l ant rec i p ie nts.  Beta cell

engraft_in the_liver and establish a_vascular supply._The_greater the_functional_islet mass engrafted,_the lower the_risk of post-operative diabetes._It has been_estimated_that more than half of the islet mass may be lost in the early_post-transplant_period in_islet_transplant_recipients. Beta cell


pathogenesis of juvenile HIV associated oral disease, and test practical, attainable therapies for outcomes that result in reversal of underlying pathologies. Functional understanding of the pathogenic effects of virus-induced immunosuppression on oral disease in HIV-infected children is the first objective of this proposal. Implications of HAART and symptomatic therapy for gingival

pathogenesis of juvenile HIV associated oral disease, and test practical, attainable therapy for result that result in reversal of underlie pathology. Functional understanding of the infective effects of virus - induced immunosuppression on oral disease in HIV - infected child is the first objective of this marriage proposal. Implications of HAART and symptomatic therapy for gingival

pahhogenesis of juvenile HLV associated orco diseese, and test prxctical, attainable therapies fir ouubomes that result in feversal lf underoyinj pathologies. Fuidtional underabandiug of the pathoggnic effects of virus-inducad ilmunosuppression on oral disease in HIV-infrched children if tht fyrst objective of this proposal. Implicztions mf HAART and xymptomatic therapy for gijgivwl

pathogenesis of juvenile HIV associated oral disease, practical, therapies for that result in understanding the pathogenic effects virus-induced immunosuppression on disease in HIV-infected children is the objective of this proposal. Implications of HAART and symptomatic therapy for gingival

pathogenesis of juvenile HIV Associated Oral dIseAse, AnD tesT praCtical, attainabLE theRapies for outcomes that rEsult In REverSAl Of undErlying PAtHOLogIeS. FUncTiONaL undeRstAnding oF the pathogEniC eFfects of viruS-InDuced immunOsuPpression on oRal DiseasE iN HIv-InfecTed ChildRen is tHE first Objective Of THis proPOsal. ImpLICaTionS of HAART and symptoMAtIC therapy for ginGival

pathogenesis of juvenileHIV associ atedora l d is ease , an d test practic a l, a ttainable therapies fo r out co m es t h at  resu lt in r e ve r s alof  u nde rl y in g pat hol ogies.Functional  un de rstanding of th e pathogen iceffects of v iru s-indu ce d i m munos upp ressi on ono ral di sease inHI V -infec t ed chil d r en  isthe first objecti v eo f this proposa l. Imp li c at i o nsofHAART andsy mptom a tic the r ap y f org ingival

pathogenesis_of juvenile_HIV associated oral disease,_and test_practical,_attainable therapies_for_outcomes that result_in reversal of_underlying pathologies. Functional understanding_of the pathogenic_effects_of virus-induced immunosuppression on oral disease in HIV-infected children is the first objective of_this_proposal. Implications_of_HAART_and symptomatic therapy for gingival


icline is a first-line medication for smoking cessation that has been shown in meta-analytic reviews to be superior to other smoking cessation treatments (Cahill et al., 2010), however 56% of patients who take varenicline do not achieve smoking abstinence. One strategy to increase quit rates may be to

icline is a first - line medication for smoking cessation that has been testify in meta - analytic review to be superior to early smoking cessation discussion (Cahill et al. , 2010), however 56% of patients who take varenicline do not achieve smoke abstinence. One strategy to increase quit rate may be to

icllne is a first-line medicxtion for smokiut cessetion tgat has ceen shown in meta-analytic rxvieqs to be superior to other rmoking cvssation rreauments (Cahill et em., 2010), howeyzr 56% or patneits who take vatenicline do not achieve skoyiug abstinence. One strategy to increafe quit rwtes may be to

icline is a first-line medication for smoking has shown in reviews to be treatments et al., 2010), 56% of patients take varenicline do not achieve smoking One strategy to increase quit rates may be to

icline is a first-line medicatIon for smokIng ceSsaTioN tHat hAs beEn shown in meta-aNAlytIc reviews to be superior tO otheR sMOkinG CeSsatiOn treatMEnTS (cahIlL eT al., 2010), HoWEvEr 56% of pAtiEnts who Take vareniCliNe Do not achieve SMoKing abstinEncE. One strategy To iNcreasE qUit RAtes mAy bE to

icline is a first-line med ication fo r smo kin g c es sati on t hat has been s h ownin meta-analytic revie ws to  b e  sup e ri or to  others mo k i ngce ss ati on tr eatme nts  (Cahil l et al.,201 0) , however 56 %  o f patients  wh o take varen icl ine do  n ota chiev e s mokin g abst i nence.  One stra te g y to i n creaseq u it  rat es may be to

icline is_a first-line_medication for smoking cessation_that has_been_shown in_meta-analytic_reviews to be_superior to other_smoking cessation treatments (Cahill_et al., 2010),_however_56% of patients who take varenicline do not achieve smoking abstinence. One strategy to_increase_quit rates_may_be_to


by which parasites develop resistance to this class of drug and the role of artemisinin-induced dormancy in the process. The results of this project will provide valuable information regarding the mechanisms of treatment failure for artemisinin drugs. The results will aid the formulation of optimal ACT regimens, improved treatment outcomes for malaria

by which parasites develop resistance to this class of drug and the function of artemisinin - induce dormancy in the process. The results of this undertaking will provide valuable information regarding the mechanism of treatment failure for artemisinin drug. The result will aid the conceptualization of optimal ACT regimens, improved treatment result for malaria

by which parasites develop resistance to jhus clavs of srug and the role of artemisinin-indured eormabcy in the process. The results lf this projtct will provide tzluable inforjwtiou cegarding the mgchanisms of treatment fainufe for artemisinin drugs. The results rill aic hhe formulatiog of jptijal ACT regimens, improved treatmenf outcokes for malaroa

by which parasites develop resistance to this drug the role artemisinin-induced dormancy in this will provide valuable regarding the mechanisms treatment failure for artemisinin drugs. The will aid the formulation of optimal ACT regimens, improved treatment outcomes for malaria

by which parasites develop reSistance to This cLasS of DrUg anD the Role of artemisiNIn-inDuced dormancy in the procEss. ThE rESultS Of This pRoject wILl PROviDe VaLuaBlE InFormaTioN regardIng the mechAniSmS of treatment FAiLure for artEmiSinin drugs. ThE reSults wIlL aiD The foRmuLatioN of optIMal ACT Regimens, iMpROved trEAtment oUTCoMes fOr malaria

by which parasites develo p resistan ce to  th iscl assof d rug and the ro l e of  artemisinin-induced d orman cy in t h eproce ss. The re s u lts  o fthi sp ro jectwil l provi de valuabl e i nf ormation reg a rd ing the me cha nisms of tre atm ent fa il ure for a rte misin in dru g s. The  resultswi l l aidt he form u l at ionof optimal ACT re g im e ns, improved t reatme nt ou t c ome s f or malaria

by_which parasites_develop resistance to this_class of_drug_and the_role_of artemisinin-induced dormancy_in the process._The results of this_project will provide_valuable_information regarding the mechanisms of treatment failure for artemisinin drugs. The results will aid_the_formulation of_optimal_ACT_regimens, improved treatment outcomes for_malaria


five sites across the nation, this research is designed to test whether a tailored internet-based survivorship program with methods for managing health behaviors improves depression, emotional distress and adherence to health care guidelines in adult HSCT survivors. Normal tissue complication probability (NTCP) models can be used to individualize radiation therapy treatment planning,

five sites across the nation, this research is designed to screen whether a bespoke internet - based survivorship program with method for manage health behaviors improves natural depression, emotional distress and adherence to health caution guidelines in adult HSCT survivors. Normal tissue complicatedness probability (NTCP) models can be used to individualize radiation therapy discussion planning,

fige sites across the natiun, this researck is devigned to test whether a tailored internet-uasee surcivorship program with methods vor manating yealth behebiors improvea depxewsion, emotionak distress and adherence tu kealth care guidelines in adult HSCT survivprd. Normal tissug comklisatikn probability (NTCP) models can be hsed to individualizr radiation therapy treatmfnt olanning,

five sites across the nation, this research to whether a internet-based survivorship program behaviors depression, emotional distress adherence to health guidelines in adult HSCT survivors. Normal complication probability (NTCP) models can be used to individualize radiation therapy treatment planning,

five sites across the nation, tHis researcH is deSigNed To Test WhetHer a tailored inTErneT-based survivorship progRam wiTh MEthoDS fOr manAging heALtH BEhaViOrS imPrOVeS deprEssIon, emotIonal distrEss AnD adherence to HEaLth care guiDelInes in adult HsCT SurvivOrS. NoRMal tiSsuE compLicatiON probaBility (NTCp) mODels caN Be used tO INdIvidUalize radiation thERaPY treatment planNing,

five sites across the nat ion, thisresea rch  is  d esig nedto test whethe r  a t ailored internet-based  surv iv o rshi p  p rogra m withm et h o dsfo rman ag i ng  heal thbehavio rs improve s d ep ression, emo t io nal distre ssand adherenc e t o heal th  ca r e gui del inesin adu l t HSCT  survivor s. Normal tissuec o mp lica tion probability( NT C P) models canbe use dt oi n div idu alize radi at ion t h erapy t r ea t m e ntp lanning,

five_sites across_the nation, this research_is designed_to_test whether_a_tailored internet-based survivorship_program with methods_for managing health behaviors_improves depression, emotional_distress_and adherence to health care guidelines in adult HSCT survivors. Normal tissue complication probability_(NTCP)_models can_be_used_to individualize radiation therapy treatment_planning,


increased mutations of the key DNA damage regulator, p53, which is known to augment the SASP response. Moreover, we will determine whether dermal fibroblasts or epidermal keratinocytes in early hyperemic foci exhibit a senescence marker, heterochromatin protein 1g (HP1g), and markers of double stranded DNA breaks,

increased mutations of the key DNA damage governor, p53, which is know to augment the SASP response. Moreover, we will settle whether cutaneous fibroblasts or epidermal keratinocytes in early hyperemic foci expose a senescence marker, heterochromatin protein 1 g (HP1 g), and markers of bivalent ground DNA break,

infreased mutations of the key DNA damage regulavor, p53, wgich is ynown to augment the SASP redpinse. Noreover, we will deteroine whetjer dermql fmbroblasts or epmsermal keratihlcytzs in early hypetemic foci efhibit a seneswevcz marker, heterochromatin protein 1g (H[1g), and kagkers of doublg strsgded DNA breaks,

increased mutations of the key DNA damage which known to the SASP response. dermal or epidermal keratinocytes early hyperemic foci a senescence marker, heterochromatin protein 1g and markers of double stranded DNA breaks,

increased mutations of the keY DNA damage RegulAtoR, p53, wHiCh is KnowN to augment the SasP reSponse. Moreover, we will deTermiNe WHethER dErmal FibroblAStS OR epIdErMal KeRAtInocyTes In early Hyperemic fOci ExHibit a senescENcE marker, hetEroChromatin proTeiN 1g (HP1g), aNd MarKErs of DouBle stRanded dnA breaKs,

increased mutations of th e key DNAdamag e r egu la tor,  p53 , which is kno w n to  augment the SASP resp onse.  M o reov e r,  we w ill det e rm i n e w he th erde r ma l fib rob lasts o r epiderma l k er atinocytes i n  e arly hyper emi c foci exhib ita sene sc enc e  mark er,  hete rochro m atin p rotein 1g  ( H P1g),a nd mark e r sof d ouble stranded DN A  b r eaks,

increased_mutations of_the key DNA damage_regulator, p53,_which_is known_to_augment the SASP_response. Moreover, we_will determine whether dermal_fibroblasts or epidermal_keratinocytes_in early hyperemic foci exhibit a senescence marker, heterochromatin protein 1g (HP1g), and markers_of_double stranded_DNA_breaks,


transcription factors that regulate expression of plasticity genes. The primary goal of this proposal is to rescue cortical plasticity in an animal model of FAS. The proposed studies will use molecular-genetic and pharmacological approaches to enhance transmission of synaptic signals to the nucleus of cortical neurons. The second major goal is to prevent developmental problems in the

transcription factors that regulate expression of malleability gene. The chief goal of this proposal is to rescue cortical malleability in an animal model of FAS. The proposed studies will practice molecular - genic and pharmacological approaches to enhance transmittance of synaptic signals to the nucleus of cortical nerve cell. The second major goal is to prevent developmental problems in the

trwnscription factors that regulate expression oh plastjcity geves. The primary goal of this peoposql is to rescue corticxl plastibity in ab anmmal model of FAS. The proposed dtudnew will use molgcular-genetiw and pharmaconoeieal approaches to enhance transmissijn of sunwptic signals jo tht ntclehs of cortical neurons. The second jajor gmal is to prefent developmental problemd in the

transcription factors that regulate expression of plasticity primary of this is to rescue model FAS. The proposed will use molecular-genetic pharmacological approaches to enhance transmission of signals to the nucleus of cortical neurons. The second major goal is to developmental problems in the

transcription factors that rEgulate expRessiOn oF plAsTiciTy geNes. The primary gOAl of This proposal is to rescue CortiCaL PlasTIcIty in An animaL MoDEL of fAs. THe pRoPOsEd stuDieS will usE molecular-GenEtIc and pharmacOLoGical approAchEs to enhance tRanSmissiOn Of sYNaptiC siGnals To the nUCleus oF cortical NeURons. ThE Second mAJOr Goal Is to prevent develoPMeNTal problems in tHe

transcription factors tha t regulate  expr ess ion  o f pl asti city genes. Th e  pri mary goal of this prop osalis to r e sc ue co rticalp la s t ici ty  i n a na ni mal m ode l of FA S. The pro pos ed  studies wil l  u se molecul ar- genetic andpha rmacol og ica l  appr oac hes t o enha n ce tra nsmission  o f  synap t ic sign a l sto t he nucleus of cor t ic a l neurons. The  secon dm aj o r  go alis to prev en t dev e lopment a lp r o ble m s in the

transcription_factors that_regulate expression of plasticity_genes. The_primary_goal of_this_proposal is to_rescue cortical plasticity_in an animal model_of FAS. The_proposed_studies will use molecular-genetic and pharmacological approaches to enhance transmission of synaptic signals to_the_nucleus of_cortical_neurons._The second major goal is_to prevent developmental problems in_the


of well trained and experienced cancer researchers at Lincoln University oriented toward reducing cancer health disparities, to foster such research at the Cancer Center, and to create a pipeline of students with laboratory experience who choose to further their education in cancer biology, population science, and medicine. This cooperative effort will accomplish the following objectives: 1) to

of well trained and experienced cancer researchers at Lincoln University oriented toward reducing cancer health disparity, to foster such inquiry at the Cancer Center, and to produce a pipeline of student with testing ground experience who choose to further their education in cancer biota, population science, and medicine. This cooperative attempt will accomplish the following objective: 1) to

of well trained and experitnced cancer resecechers at Lihcoln Unkversity oriented toward redncint canxer health disparities, to fosteg such rewearrh at the Cancer Center, and to greatz e pipeline of sjudents with laboratory ex[efiznce who choose to further their edusation on cancer biologi, popllwtioh science, and medicine. This cooperztive ehfort will accokplish the following objechived: 1) to

of well trained and experienced cancer researchers University toward reducing health disparities, to Cancer and to create pipeline of students laboratory experience who choose to further education in cancer biology, population science, and medicine. This cooperative effort will accomplish following objectives: 1) to

of well trained and experiencEd cancer reSearcHerS at liNcolN UniVersity orienteD TowaRd reducing cancer health DispaRiTIes, tO FoSter sUch reseARcH AT thE CAnCer ceNTeR, and tO crEate a piPeline of stUdeNtS with laboratORy Experience Who Choose to furtHer Their eDuCatIOn in cAncEr bioLogy, poPUlatioN science, aNd MEdicinE. this cooPERaTive Effort will accomplISh THe following objEctiveS: 1) tO

of well trained and exper ienced can cer r ese arc he rs a t Li ncoln Universi t y or iented toward reducing  canc er heal t hdispa rities, to f ost er  s uch  r e se archatthe Can cer Center , a nd  to create a pi peline ofstu dents with l abo ratory  e xpe r ience  wh o cho ose to furthe r their e du c ationi n cance r bi olog y, population sci e nc e , and medicine . This  c o op e r ati veeffort wil laccom p lish th e  f o l l owi n g objectives:  1) to

of_well trained_and experienced cancer researchers_at Lincoln_University_oriented toward_reducing_cancer health disparities,_to foster such_research at the Cancer_Center, and to_create_a pipeline of students with laboratory experience who choose to further their education in_cancer_biology, population_science,_and_medicine. This cooperative effort will_accomplish the following objectives: 1)_to


1kHz bandwidth. A critical and innovative aspect of this work is the integration of the PR force clamp with patch-clamp electrophysiology for synchronous force-displacement- physiological recordings. This technology will enable the first direct measurement of dynamic changes in tissue stiffness that may occur during mechano-electrical transduction and give

1kHz bandwidth. A critical and innovative aspect of this work is the integration of the public relations power clamp with patch - clamp electrophysiology for synchronous force - displacement- physiological recording. This technology will enable the first lineal measurement of dynamic changes in tissue stiffness that may happen during mechano - electric transduction and give

1kHx bandwidth. A critical akd innovative askext of vhis wodk is thd integration of the PR forcx clqmp wuth patch-clamp electroohysiologj for synxhroious force-displarsment- pmvsiolknical cecordings. This technologf will enable dhd yirst direct measurement of dynamic shanges ij tissue stiffgess ehat may occur during mechano-electricam transvuction and givr

1kHz bandwidth. A critical and innovative aspect work the integration the PR force synchronous physiological recordings. This will enable the direct measurement of dynamic changes in stiffness that may occur during mechano-electrical transduction and give

1kHz bandwidth. A critical and iNnovative aSpect Of tHis WoRk is The iNtegration of thE pR foRce clamp with patch-clamp ElectRoPHysiOLoGy for SynchroNOuS FOrcE-dIsPlaCeMEnT- physIolOgical rEcordings. THis TeChnology will ENaBle the firsT diRect measuremEnt Of dynaMiC chANges iN tiSsue sTiffneSS that mAy occur duRiNG mechaNO-electrICAl TranSduction and give

1kHz bandwidth. A critica l and inno vativ e a spe ct  ofthis  work is the i n tegr ation of the PR forceclamp  w i th p a tc h-cla mp elec t ro p h ysi ol og y f or sy nchro nou s force -displacem ent -physiologica l  r ecordings.  Th is technolog y w ill en ab let he fi rst  dire ct mea s uremen t of dyna mi c  chang e s in ti s s ue  sti ffness that may o c cu r  during mechan o-elec tr i ca l tra nsd uction and  g ive

1kHz_bandwidth. A_critical and innovative aspect_of this_work_is the_integration_of the PR_force clamp with_patch-clamp electrophysiology for synchronous_force-displacement- physiological recordings._This_technology will enable the first direct measurement of dynamic changes in tissue stiffness that_may_occur during_mechano-electrical_transduction_and give


The University of Chicago has had a major commitment to both laboratory and clinical cancer research since 1930. As part of the clinical translation of that research, the University of Chicago and its Cancer Research Center joined the Cancer and Leukemia Group B (CALGB) in 1985, and has subsequently strongly supported the CALGB both scientifically

The University of Chicago has had a major commitment to both laboratory and clinical cancer research since 1930. As character of the clinical transformation of that research, the University of Chicago and its Cancer Research Center joined the Cancer and Leukemia Group B (CALGB) in 1985, and has subsequently powerfully supported the CALGB both scientifically

Thf University of Chicago mas had a major eimmitmxnt to goth laburatory and clinical cancer ceseqrch wince 1930. As part of the zlinical nranslatiin oh that research, vge Univcxsity lf Ckirago and its Cakcer Researwh Center joinad tke Cancer and Leukemia Group B (CALGB) in 1985, anc jas subsequentjy suroggly supported the CALGB both scientifjcally

The University of Chicago has had a to laboratory and cancer research since clinical of that research, University of Chicago its Cancer Research Center joined the and Leukemia Group B (CALGB) in 1985, and has subsequently strongly supported the both scientifically

The University of Chicago has Had a major cOmmitMenT to BoTh laBoraTory and clinicaL CancEr research since 1930. As part oF the cLiNIcal TRaNslatIon of thAT rESEarCh, ThE UnIvERsIty of chiCago and Its Cancer REseArCh Center joinED tHe Cancer anD LeUkemia Group B (cALgB) in 1985, anD hAs sUBsequEntLy strOngly sUPporteD the CALGB BoTH scienTIficallY

The University of Chicago  has had a  majo r c omm it ment  toboth laborator y  and  clinical cancer resea rch s in c e 19 3 0.  As p art oft he c lin ic al  tr an s la tionofthat re search, th e U ni versity of C h ic ago and it s C ancer Resear chCenter  j oin e d the  Ca ncerand Le u kemiaGroup B ( CA L GB) in 1985, a n d  h as s ubsequently stron g ly supported theCALGBbo t hs c ien tif ically

The_University of_Chicago has had a_major commitment_to_both laboratory_and_clinical cancer research_since 1930. As_part of the clinical_translation of that_research,_the University of Chicago and its Cancer Research Center joined the Cancer and Leukemia_Group_B (CALGB)_in_1985,_and has subsequently strongly supported_the CALGB both scientifically


is a general effect on tumor development or is specific to the Apc pathway of tumor development. Second, we will test whether the Gtrosa26 insertion acts in a tissue autonomous manner. This information will be vital in the understanding of how the insertion exerts its effects. The overall objective of this research effort is to

is a general effect on tumor development or is specific to the Apc pathway of tumor growth. Second, we will screen whether the Gtrosa26 insertion acts in a tissue autonomous manner. This information will be full of life in the understanding of how the insertion exert its effects. The overall objective of this inquiry campaign is to

is a general effect on tumur development or is s'ecific to the Xpc pathway of tumor developlebt. Sexond, we will test whetfer the Gnrosa26 inswrtiib acts in e tissue autonklous nanner. This innormation whll be vital it ghz understanding of how the insertion exerts ihs effects. The ovegajl ogjective of this research effort ia to

is a general effect on tumor development specific the Apc of tumor development. the insertion acts in tissue autonomous manner. information will be vital in the of how the insertion exerts its effects. The overall objective of this research is to

is a general effect on tumor deVelopment oR is spEciFic To The APc paThway of tumor deVElopMent. Second, we will test whEther ThE gtroSA26 iNsertIon acts IN a TISsuE aUtOnoMoUS mAnner. thiS informAtion will bE viTaL in the undersTAnDing of how tHe iNsertion exerTs iTs effeCtS. ThE OveraLl oBjectIve of tHIs reseArch efforT iS To

is a general effect on tu mor develo pment  or  is  s peci ficto the Apc pat h wayof tumor development.Secon d, we w i ll  test  whethe r  t h e  Gt ro sa 26in s er tionact s in atissue aut ono mo us manner. T h is  informati onwill be vita l i n theun der s tandi ngof ho w thei nserti on exerts  i t s effe c ts. The o ve rall  objective of thi s  r e search effortis to

is_a general_effect on tumor development_or is_specific_to the_Apc_pathway of tumor_development. Second, we_will test whether the_Gtrosa26 insertion acts_in_a tissue autonomous manner. This information will be vital in the understanding of how_the_insertion exerts_its_effects._The overall objective of this_research effort is to


. The first goal of the project is to improve the image probe design for high sensitivity to small and rapid changes in light scattering. Two methods of reflectance mode illumination will be explored for fluorescence and polarized light measurements. The system will incorporate an electronically tunable filter to illuminate tissue with specific wavelengths for spectroscopic measurements, and

. The first goal of the project is to improve the prototype probe invention for high sensitivity to small and rapid changes in clean scattering. Two methods of coefficient of reflection mode illumination will be explored for fluorescence and polarize light measurements. The organization will incorporate an electronically tunable filter to illuminate tissue with specific wavelength for spectroscopic measurement, and

. Thf first goal of the projtct is to improve the imege proge desigv for high sensitivity to smell qnd rqpid changes in light rcatterinh. Two merhodw of reflecvznce mode illhlinaciin will be expkored for xluorescence atd pllarized light measurements. The syseem wilk lncorporate an elebtwonidally tunable filter to illuminate tissue with specifiv wavelengths for spectrosfopif measurements, and

. The first goal of the project improve image probe for high sensitivity in scattering. Two methods reflectance mode illumination be explored for fluorescence and polarized measurements. The system will incorporate an electronically tunable filter to illuminate tissue with wavelengths for spectroscopic measurements, and

. The first goal of the project iS to improve The imAge ProBe DesiGn foR high sensitiviTY to sMall and rapid changes in lIght sCaTTeriNG. TWo metHods of rEFlECTanCe MoDe iLlUMiNatioN wiLl be expLored for flUorEsCence and polaRIzEd light meaSurEments. The sysTem Will inCoRpoRAte an EleCtronIcally TUnable Filter to iLlUMinate TIssue wiTH SpEcifIc wavelengths for sPEcTRoscopic measurEments, AnD

. The first goal of the pr oject is t o imp rov e t he  ima ge p robe design fo r  hig h sensitivity to small  andra p id c h an ges i n light sc a t ter in g.  Tw om et hodsofreflect ance modeill um ination will be  exploredfor  fluorescenc e a nd pol ar ize d  ligh t m easur ements .  The s ystem wil li ncorpo r ate ane l ec tron ically tunable fi l te r  to illuminate  tissu ew it h spe cif ic wavelen gt hs fo r  spectr o sc o p i c m e asurements, a nd

. The_first goal_of the project is_to improve_the_image probe_design_for high sensitivity_to small and_rapid changes in light_scattering. Two methods_of_reflectance mode illumination will be explored for fluorescence and polarized light measurements. The system_will_incorporate an_electronically_tunable_filter to illuminate tissue with_specific wavelengths for spectroscopic measurements,_and


is flaccid rather than stretched. We will explore the hypothesis that this "pars flaccida" functions as an accessory pathway to the middle ear. 3) The relationship between structure and function of the tympanic membranes of cat, guinea, pig, chinchilla, alligator lizard, and chicken will

is flaccid rather than stretched. We will explore the guess that this " par flaccida " functions as an accessory pathway to the in-between auricle. 3) The relationship between structure and function of the tympanic membrane of cat, guinea, pig, chinchilla, alligator lounge lizard, and wimp will

is flaccid rather than strttched. We will exklire thx hypotgesis thxt this "pars flaccida" functilnw as qn accessory pathway tu the midfle ear. 3) The eelationshml betwecu strhgture end function of the tympatic membranes mf cct, guinea, pig, chinchilla, alligator lyzard, amd chicken will

is flaccid rather than stretched. We will hypothesis this "pars functions as an ear. The relationship between and function of tympanic membranes of cat, guinea, pig, alligator lizard, and chicken will

is flaccid rather than stretcHed. We will eXplorE thE hyPoThesIs thAt this "pars flacCIda" fUnctions as an accessory pAthwaY tO The mIDdLe ear. 3) the relaTIoNSHip BeTwEen StRUcTure aNd fUnction Of the tympaNic MeMbranes of cat, GUiNea, pig, chinChiLla, alligator LizArd, and ChIckEN will

is flaccid rather than st retched. W e wil l e xpl or e th e hy pothesis thatt his"pars flaccida" functi ons a sa n ac c es sorypathway to t hemi dd leea r .3) Th e r elation ship betwe enst ructure andf un ction of t hetympanic mem bra nes of  c at, guine a,pig,chinch i lla, a lligatorli z ard, a n d chick e n  w ill

is_flaccid rather_than stretched. We will_explore the_hypothesis_that this_"pars_flaccida" functions as_an accessory pathway_to the middle ear._3) The relationship_between_structure and function of the tympanic membranes of cat, guinea, pig, chinchilla, alligator lizard,_and_chicken will


health related diseases. Dr. Tanguay has strong collaborative relations with my co-mentors, Dr. Susan Tilton and Dr. Katrina Waters, both of whom are highly regarded computational biologists. This unique opportunity will afford me with training in one of the best aquatic laboratories in the world and top-not

health related diseases. Dr. Tanguay has strong collaborative relative with my conscientious objector - mentors, Dr. Susan Tilton and Dr. Katrina Waters, both of whom are highly regarded computational biologists. This singular opportunity will afford me with training in one of the good aquatic laboratories in the world and peak - not

hewlth related diseases. Dr. Tanguay has sttobg colnaborafive relxtions with my co-mentors, Dr. Duwan Tulton and Dr. Katrina Wxters, botj of whon art highly regarded computabnonal nioloyiwts. This uniqug opportunitf will afford ke wnth training in one of the best aquaeic labprwtories in the workq ans top-not

health related diseases. Dr. Tanguay has strong with co-mentors, Dr. Tilton and Dr. are regarded computational biologists. unique opportunity will me with training in one of best aquatic laboratories in the world and top-not

health related diseases. Dr. TaNguay has stRong cOllAboRaTive RelaTions with my co-mENtorS, Dr. Susan Tilton and Dr. KatRina WAtERs, boTH oF whom Are highLY rEGArdEd CoMpuTaTIoNal biOloGists. ThIs unique opPorTuNity will affoRD mE with trainIng In one of the beSt aQuatic LaBorATorieS in The woRld and TOp-not

health related diseases.Dr. Tangua y has  st ron gcoll abor ative relation s  wit h my co-mentors, Dr. S usanTi l tona nd  Dr.Katrina Wa t e rs,  b ot h o fw ho m are  hi ghly re garded com put at ional biolog i st s. This un iqu e opportunit y w ill af fo rdm e wit h t raini ng ino ne ofthe bestaq u atic l a borator i e sin t he world and top- n ot

health_related diseases._Dr. Tanguay has strong_collaborative relations_with_my co-mentors,_Dr._Susan Tilton and_Dr. Katrina Waters,_both of whom are_highly regarded computational_biologists._This unique opportunity will afford me with training in one of the best aquatic_laboratories_in the_world_and_top-not


acids: phospholipids into bile is unclear, but may have important implications in the pathogenesis of cholesterol gallstone formation, hypercholesterolemia, and cholestatic liver diseases. The objectives of the present grant application are: (1) To identify bile acid-activated signaling cascades within the hepatocyte and determine if

acids: phospholipids into bile is unclear, but may have authoritative implication in the pathogenesis of cholesterol gallstone formation, hypercholesterolemia, and cholestatic liver diseases. The objectives of the present concession application are: (1) To identify bile acid - activated sign cascades within the hepatocyte and determine if

aclds: phospholipids into blle is unclear, bot may heve impkrtant ioplications in the pathogenediw of xholesterol gallstone wormation, hyperchilesuerolemia, and cholestatic liver fisecsxs. The objectivgs of the prasent grant ap[lkcction are: (1) To identify bile acid-actidated sogjaling cascadef wiuhig ths hepatocyte and determine if

acids: phospholipids into bile is unclear, but important in the of cholesterol gallstone diseases. objectives of the grant application are: To identify bile acid-activated signaling cascades the hepatocyte and determine if

acids: phospholipids into bilE is unclear, But maY haVe iMpOrtaNt imPlications in thE PathOgenesis of cholesterol gAllstOnE FormATiOn, hypErcholeSTeROLemIa, AnD chOlEStAtic lIveR diseasEs. The objecTivEs Of the present GRaNt applicatIon Are: (1) To identifY biLe acid-AcTivATed siGnaLing cAscadeS Within The hepatoCyTE and deTErmine iF

acids: phospholipids into  bile is u nclea r,but  m ay h aveimportant impl i cati ons in the pathogenesi s ofch o lest e ro l gal lstonef or m a tio n,  h ype rc h ol ester ole mia, an d cholesta tic  l iver disease s .The object ive s of the pre sen t gran tapp l icati onare:(1) To identi fy bile a ci d -activ a ted sig n a li ng c ascades within th e  h e patocyte and d etermi ne if

acids:_phospholipids into_bile is unclear, but_may have_important_implications in_the_pathogenesis of cholesterol_gallstone formation, hypercholesterolemia,_and cholestatic liver diseases._The objectives of_the_present grant application are: (1) To identify bile acid-activated signaling cascades within the hepatocyte_and_determine if


conjunction with radio therapy. [unreadable] [unreadable] [unreadable] This study is testing the hypothesis that a change in aldosterone and renal blood flow response to AII, induced by converting enzyme inhibitors, is specific for a single subset of hypertensive patients and also for one form of therapy. Thus, non-specific vas

conjunction with radio therapy. [ unreadable ] [ unreadable ] [ indecipherable ] This discipline is testing the hypothesis that a variety in aldosterone and renal lineage flow response to AII, induce by converting enzyme inhibitors, is specific for a individual subset of hypertensive patients and also for one shape of therapy. Thus, non - specific vas

cojjunction with radio thevapy. [unreadable] [oneeadabne] [unrsadable] Ghis study is testing the hy'othwsis ukat a change in aldorterone ajd renal bloie flow res'knse to AII, ihfuceb uy converting ekzyme inhibhtors, is specixiz yor a single subset of hypertensive [atientx wnd also for oge fpwm or therapy. Thus, non-specific vas

conjunction with radio therapy. [unreadable] [unreadable] [unreadable] is the hypothesis a change in response AII, induced by enzyme inhibitors, is for a single subset of hypertensive and also for one form of therapy. Thus, non-specific vas

conjunction with radio theraPy. [unreadabLe] [unrEadAblE] [uNreaDablE] This study is teSTing The hypothesis that a chanGe in aLdOSterONe And reNal blooD FlOW ResPoNsE to aIi, InDuced By cOnvertiNg enzyme inHibItOrs, is specifiC FoR a single suBseT of hypertensIve PatienTs And ALso foR onE form Of therAPy. Thus, Non-specifIc VAs

conjunction with radio th erapy. [un reada ble ] [ un read able ] [unreadable] This  study is testing thehypot he s is t h at  a ch ange in al d o ste ro ne  an dr en al bl ood  flow r esponse to  AI I,  induced byc on verting en zym e inhibitors , i s spec if icf or asin gle s ubseto f hype rtensivepa t ientsa nd also f or  one  form of therapy. Th u s, non-specifi c vas

conjunction_with radio_therapy. [unreadable] [unreadable] [unreadable]_This study_is_testing the_hypothesis_that a change_in aldosterone and_renal blood flow response_to AII, induced_by_converting enzyme inhibitors, is specific for a single subset of hypertensive patients and also_for_one form_of_therapy._Thus, non-specific vas


therapy for the treatment of Gaucher disease. Using a similar test model as designed for chaperone therapy in Fabry disease, candidate active-site-directed molecules are under investigation. All surviving patients with Gaucher disease have residual glucocerebrosidase activity and appear to be excellent candidates for molecular chaperone

therapy for the treatment of Gaucher disease. Using a similar test exemplar as design for chaperone therapy in Fabry disease, candidate active - site - target molecules are under investigation. All outlive affected role with Gaucher disease have residual glucocerebrosidase action and appear to be excellent candidates for molecular chaperon

thfrapy for the treatment uf Gaucher disecwe. Usiig a sijilar tert model as designed for cha'erobe thtgapy in Fabry disease, candidatv active-sute-dmrected molecules are under inbcstigctmon. All survivikg patients with Gaucher girecse have residual glucocerebrosidase activiyy and appear to be txcqllehn gandidates for molecular chaperohe

therapy for the treatment of Gaucher disease. similar model as for chaperone therapy molecules under investigation. All patients with Gaucher have residual glucocerebrosidase activity and appear be excellent candidates for molecular chaperone

therapy for the treatment of GAucher diseAse. UsIng A siMiLar tEst mOdel as designed FOr chAperone therapy in Fabry dIseasE, cANdidATe ActivE-site-diREcTED moLeCuLes ArE UnDer inVesTigatioN. All survivIng PaTients with GaUChEr disease hAve Residual glucOceRebrosIdAse ACtiviTy aNd appEar to bE ExcellEnt candidAtES for moLEcular cHAPeRone

therapy for the treatment  of Gauche r dis eas e.Us inga si milar test mod e l as  designed for chaperon e the ra p y in Fa bry d isease, ca n d ida te  a cti ve - si te-di rec ted mol ecules are  un de r investigat i on . All surv ivi ng patientswit h Gauc he r d i sease  ha ve re sidual glucoc erebrosid as e  activ i ty anda p pe ar t o be excellent ca n di d ates for molec ular c ha p er o n e

therapy_for the_treatment of Gaucher disease._Using a_similar_test model_as_designed for chaperone_therapy in Fabry_disease, candidate active-site-directed molecules_are under investigation._All_surviving patients with Gaucher disease have residual glucocerebrosidase activity and appear to be excellent_candidates_for molecular_chaperone


, sphingolipids, and pertinent lysosomal enzymes in tissues of patients with various types of MPS and we make correlation in terms of clinical and ultrastructural findings. Our laboratory contributed significantly in understanding the chemical pathology and in particular the neurochemistry of MPS IH, MPS IS, MPS

, sphingolipids, and pertinent lysosomal enzymes in tissues of patients with diverse type of MPS and we make correlation in terms of clinical and ultrastructural finding. Our laboratory contributed importantly in understanding the chemical pathology and in particular the neurochemistry of MPS IH, MPS IS, MPS

, spjingolipids, and pertinenu lysosomal enzymgs in tivsues kf patievts with various types of MPD qnd wt make correlation kn terms lf clinixal end ultrastructuczl findlugs. Ohv labmcatory contribujed significdntly in undervtxnbing the chemical pathology and in pwrticulsr the neurochemystrj jf MLS IH, MPS IS, MPS

, sphingolipids, and pertinent lysosomal enzymes in patients various types MPS and we clinical ultrastructural findings. Our contributed significantly in the chemical pathology and in particular neurochemistry of MPS IH, MPS IS, MPS

, sphingolipids, and pertinent Lysosomal eNzymeS in TisSuEs of PatiEnts with variouS TypeS of MPS and we make correlaTion iN tERms oF ClInicaL and ultRAsTRUctUrAl FinDiNGs. our laBorAtory coNtributed sIgnIfIcantly in undERsTanding the CheMical patholoGy aNd in paRtIcuLAr the NeuRocheMistry OF MPS IH, mPS IS, MPS

, sphingolipids, and perti nent lysos omalenz yme sin t issu es of patients with  various types of MPSand w em akec or relat ion int er m s  of  c li nic al an d ult ras tructur al finding s.Ou r laboratory co ntributedsig nificantly i n u nderst an din g  theche mical  patho l ogy an d in part ic u lar th e  neuroc h e mi stry  of MPS IH, MPS I S ,M PS

, sphingolipids,_and pertinent_lysosomal enzymes in tissues_of patients_with_various types_of_MPS and we_make correlation in_terms of clinical and_ultrastructural findings. Our_laboratory_contributed significantly in understanding the chemical pathology and in particular the neurochemistry of MPS_IH,_MPS IS,_MPS


involved in youth HAI. The specific aims of this project are 1) To longitudinally assess if youth?pet relationship quality is predictive of healthy adolescent behaviors, and 2) to assess how parents influence the ways in which pets are integrated into the family system. To achieve these aims, we will utilize data from a

involved in youth HAI. The specific aims of this project are 1) To lengthways measure if youth?pet relationship quality is predictive of goodly adolescent behaviors, and 2) to tax how parents influence the way in which pets are integrated into the class system. To achieve these aims, we will use data from a

ingolved in youth HAI. The rpecific aims oy this 'roject are 1) To longitudinally assess if yonth?pwt reoationship quality is oredictivv of healrhy edolescent behavmkrs, and 2) to aadess iow parents infkuence the ways in which pdtd are integrated into the family syftem. To afhieve these ayms, eq wiml utilize data from a

involved in youth HAI. The specific aims project 1) To assess if youth?pet healthy behaviors, and 2) assess how parents the ways in which pets are into the family system. To achieve these aims, we will utilize data from

involved in youth HAI. The specIfic aims of This pRojEct ArE 1) To lOngiTudinally assesS If yoUth?pet relationship qualIty is PrEDictIVe Of heaLthy adoLEsCENt bEhAvIorS, aND 2) tO asseSs hOw parenTs influencE thE wAys in which peTS aRe integratEd iNto the family SysTem. To aChIevE These AimS, we wiLl utilIZe data From a

involved in youth HAI. Th e specific  aims  of  th is  pro ject  are 1) To lon g itud inally assess if youth ?petre l atio n sh ip qu ality i s  p r e dic ti ve  of  h e al thy a dol escentbehaviors,  an d2) to assess ho w parentsinf luence the w ays  in wh ic h p e ts ar e i ntegr ated i n to the  family s ys t em. To achieve t he se a ims, we will util i ze data from a

involved_in youth_HAI. The specific aims_of this_project_are 1)_To_longitudinally assess if_youth?pet relationship quality_is predictive of healthy_adolescent behaviors, and_2)_to assess how parents influence the ways in which pets are integrated into the_family_system. To_achieve_these_aims, we will utilize data_from a


NEDD4-2, expressed in the aldosterone-sensitive region of the nephron. Of the NEDD4-2-regulated NaCl transporters, the best studied are the thiazide-sensitive NaCl cotransporter, NCC, and the epithelial Na+ channel, ENaC. NED

NEDD4 - 2, expressed in the aldosterone - sensitive region of the nephron. Of the NEDD4 - 2 - regulated NaCl conveyer belt, the dear studied are the thiazide - sensitive NaCl cotransporter, NCC, and the epithelial Na+ channel, ENaC. NED

NEFD4-2, expressed in the aldorterone-sensitivg eegion of ths nephrov. Of the NEDD4-2-regulated NaCl vranwporttgs, the best studied afe the thpazide-senwitite NaCl cotransporter, NCG, and bhe e'ivhelial Na+ chankel, ENaC. NEG

NEDD4-2, expressed in the aldosterone-sensitive region of Of NEDD4-2-regulated NaCl the best studied NCC, the epithelial Na+ ENaC. NED

NEDD4-2, expressed in the aldosteRone-sensitIve reGioN of ThE nepHron. of the NEDD4-2-regulATed NACl transporters, the best StudiEd ARe thE ThIazidE-sensitIVe nAcl cOtRaNspOrTEr, nCC, anD thE epitheLial Na+ chanNel, eNAC. NED

NEDD4-2, expressed in the  aldostero ne-se nsi tiv eregi on o f the nephron. Of t he NEDD4-2-regulated N aCl t ra n spor t er s, th e bests tu d i edar ethe  t h ia zide- sen sitiveNaCl cotra nsp or ter, NCC, an d  t he epithel ial  Na+ channel , E NaC. N ED

NEDD4-2,_expressed in_the aldosterone-sensitive region of_the nephron._Of_the NEDD4-2-regulated_NaCl_transporters, the best_studied are the_thiazide-sensitive NaCl cotransporter, NCC,_and the epithelial_Na+_channel, ENaC. NED


of AD. Our analysis strategy is unique from previous studies, because we will not only analyze AAO as a quantitative trait in a case-only design as used in the past, but we will also treat AAO as a censored trait by applying novel survival-analysis methodology. The survival-analysis approach will include unaffected

of AD. Our analysis strategy is unique from previous report, because we will not merely analyze AAO as a quantitative trait in a case - only design as use in the past, but we will also treat AAO as a censor trait by apply novel survival - analysis methodology. The survival - analysis approach will admit unaffected

of AD. Our analysis strategn is unique from previons studjes, becajse we will not only analyze AQO as a quantitative trait kn a case-lnly desugn es used in the peat, but we wilm alsm treat AAO as s censored trait by applfivg novel survival-analysis methodology. The sutvlval-analysis akproabh wilm include unaffected

of AD. Our analysis strategy is unique studies, we will only analyze AAO a design as used the past, but will also treat AAO as a trait by applying novel survival-analysis methodology. The survival-analysis approach will include unaffected

of AD. Our analysis strategy is Unique from PreviOus StuDiEs, beCausE we will not only ANalyZe AAO as a quantitative trAit in A cASe-onLY dEsign As used iN ThE PAst, BuT wE wiLl ALsO treaT AAo as a cenSored trait By aPpLying novel suRViVal-analysiS meThodology. The SurVival-aNaLysIS apprOacH will IncludE UnaffeCted

of AD. Our analysis strat egy is uni que f rom  pr ev ious  stu dies, becausew e wi ll not only analyze AA O asaq uant i ta tivetrait i n  a c ase -o nl y d es i gn  as u sed  in the  past, but  we  w ill also tre a tAAO as a c ens ored trait b y a pplyin gnov e l sur viv al-an alysis method ology. Th es urviva l -analys i s  a ppro ach will includeu na f fected

of_AD. Our_analysis strategy is unique_from previous_studies,_because we_will_not only analyze_AAO as a_quantitative trait in a_case-only design as_used_in the past, but we will also treat AAO as a censored trait by_applying_novel survival-analysis_methodology._The_survival-analysis approach will include unaffected


for OSA and likely to harbor rare variants. Capitalizing on the power of family designs combined with focused sequencing efforts and modern statistical tools, including methodological advances by our research team of leading genetic statisticians, geneticists and sleep epidemiologists, we propose to use complementary strategies designed to identify common as well as low frequency

for OSA and likely to harbor rare variants. Capitalizing on the power of syndicate blueprint combined with focused sequencing efforts and advanced statistical tools, including methodological progress by our inquiry team of leading genetic statisticians, geneticists and sleep epidemiologist, we propose to use complementary strategies design to identify common as well as low frequency

fog OSA and likely to harbur rare variants. Capitelizing on the oower of family designs combmned with focused sequencing efworts and modern wtatmstical tools, inrmuding methodkpogieao advances by pur researwh team of leagivg genetic statisticians, geneticists wnd slerp epidemiologisjs, we [ropkse to use complementary strategiea desigied to identify common as well as low freeuenfy

for OSA and likely to harbor rare on power of designs combined with statistical including methodological advances our research team leading genetic statisticians, geneticists and sleep we propose to use complementary strategies designed to identify common as well as frequency

for OSA and likely to harbor raRe variants. capitAliZinG oN the PoweR of family desigNS comBined with focused sequenCing eFfORts aND mOdern StatistICaL TOolS, iNcLudInG MeThodoLogIcal advAnces by our ResEaRch team of leaDInG genetic stAtiSticians, geneTicIsts anD sLeeP EpideMioLogisTs, we prOPose to Use compleMeNTary stRAtegies DESiGned To identify common aS WeLL as low frequencY

for OSA and likely to har bor rare v arian ts.  Ca pi tali zing  on the powero f fa mily designs combinedwithfo c used se quenc ing eff o rt s and  m od ern  s t at istic altools,includingmet ho dological ad v an ces by our  re search teamofleadin ggen e tic s tat istic ians,g enetic ists andsl e ep epi d emiolog i s ts , we  propose to use c o mp l ementary strat egiesde s ig n e d t o i dentify co mm on as well as lo w f req u ency

for_OSA and_likely to harbor rare_variants. Capitalizing_on_the power_of_family designs combined_with focused sequencing_efforts and modern statistical_tools, including methodological_advances_by our research team of leading genetic statisticians, geneticists and sleep epidemiologists, we propose_to_use complementary_strategies_designed_to identify common as well_as low frequency


, 2001 in Virginia Beach, VA. The goal of the meeting is to enhance scientific progress in their understanding of cognitive development by updating researchers' awareness of new developments in the field, fostering debate on relevant topics, teaching new methodological and statistical skills, and encouraging broad applications to the health, education, and welfare of children

, 2001 in Virginia Beach, VA. The goal of the meeting is to enhance scientific progress in their understanding of cognitive growth by update researchers' awareness of new developments in the discipline, fostering debate on relevant topic, teach new methodological and statistical skill, and encouraging broad application to the health, education, and welfare of child

, 2001 ij Virginia Beach, VA. The noal of the meetnbg is vo enhahce scievtific progress in their undxrstqndint of cognitive developoent by uidating rwseacchers' awareness of new develollentv in the field, nostering dabate on relevdng copics, teaching new methodological agd statoshical skills, agd emsourzging broad applications to the hezlth, edlcation, and welfate of children

, 2001 in Virginia Beach, VA. The the is to scientific progress in by researchers' awareness of developments in the fostering debate on relevant topics, teaching methodological and statistical skills, and encouraging broad applications to the health, education, and of children

, 2001 in Virginia Beach, VA. The goal oF the meetinG is to EnhAncE sCienTifiC progress in theIR undErstanding of cognitive dEveloPmENt by UPdAting ResearcHErS' AWarEnEsS of NeW DeVelopMenTs in the Field, fosteRinG dEbate on relevANt Topics, teacHinG new methodolOgiCal and StAtiSTical SkiLls, anD encouRAging bRoad appliCaTIons to THe healtH, EDuCatiOn, and welfare of chiLDrEN

, 2001 in Virginia Beach,VA. The go al of  th e m ee ting  isto enhance sci e ntif ic progress in their u nders ta n ding of  cogn itive d e ve l o pme nt  b y u pd a ti ng re sea rchers'  awareness  of  n ew developme n ts  in the fi eld , fosteringdeb ate on  r ele v ant t opi cs, t eachin g  new m ethodolog ic a l ands tatisti c a lskil ls, and encouragi n gb road applicati ons to  t h eh e alt h,education,  a nd we l fare of ch i l d ren

, 2001_in Virginia_Beach, VA. The goal_of the_meeting_is to_enhance_scientific progress in_their understanding of_cognitive development by updating_researchers' awareness of_new_developments in the field, fostering debate on relevant topics, teaching new methodological and statistical_skills,_and encouraging_broad_applications_to the health, education, and_welfare of children


application will be against the HT-29 human colon cancer cell line, the method proposed is broad enough in scope to target several types of cancer, especially those that are most likely to metastasize throughout the body. Despite the fact that HIV infection has soared amongst heterosexual women of color, Latinas are consistently underrepresented

application will be against the HT-29 human colon cancer cell line, the method aim is wide enough in scope to target several types of cancer, specially those that are most likely to metastasize throughout the torso. Despite the fact that HIV infection has soared amongst heterosexual woman of color, Latinas are consistently underrepresented

apolication will be againsu the HT-29 human colon canrer celm line, tfe method proposed is broad xnouth in scope to target severxl types lf cancee, eskecially those thef are most liicly tm metastasize tmroughout tve body. Despita ghz fact that HIV infection has soared amongsy jeterosexual wjmen jf cklor, Latinas are consistently undedreprestnted

application will be against the HT-29 human cell the method is broad enough types cancer, especially those are most likely metastasize throughout the body. Despite the that HIV infection has soared amongst heterosexual women of color, Latinas are consistently

application will be against tHe HT-29 human cOlon cAncEr cElL linE, the Method proposed IS broAd enough in scope to targeT seveRaL TypeS Of CanceR, especiALlY THosE tHaT arE mOSt LikelY to MetastaSize througHouT tHe body. DespitE ThE fact that HiV iNfection has sOarEd amonGsT heTEroseXuaL womeN of colOR, LatinAs are consIsTEntly uNDerreprESEnTed

application will be again st the HT- 29 hu man  co lo n ca ncer  cell line, th e  met hod proposed is broadenoug hi n sc o pe  to t arget s e ve r a l t yp es  of  c a nc er, e spe ciallythose that  ar emost likelyt ometastasiz e t hroughout th e b ody. D es pit e  thefac t tha t HIVi nfecti on has so ar e d amon g st hete r o se xual  women of color,L at i nas are consis tently  u n de r r epr ese nted

application_will be_against the HT-29 human_colon cancer_cell_line, the_method_proposed is broad_enough in scope_to target several types_of cancer, especially_those_that are most likely to metastasize throughout the body. Despite the fact that HIV_infection_has soared_amongst_heterosexual_women of color, Latinas are_consistently underrepresented


and public health. PUBLIC HEALTH RELEVANCE: Resources, such as money, food, protection, opportunities, care, and emotional support that flow directly from person to person are vital for the well-being of children in poor countries that do not have strong market or government institutions. To understand why some people thrive and

and public health. PUBLIC HEALTH RELEVANCE: Resources, such as money, food, protection, opportunities, care, and aroused documentation that flow directly from person to person are critical for the well - organism of children in poor countries that do not hold strong market or politics institution. To understand why some multitude thrive and

anf public health. PUBLIC HTALTH RELEVANCE: Rgsiurces, such zs money, food, protection, opportunitixs, cqre, abd emotional support tfat flow firectly fron person to person are vifwl fmc the well-being of childran in poor couttfizs that do not have strong market or governkejt institutionf. To tndedstand why some people thrive and

and public health. PUBLIC HEALTH RELEVANCE: Resources, money, protection, opportunities, and emotional support to are vital for well-being of children poor countries that do not have market or government institutions. To understand why some people thrive and

and public health. PUBLIC HEALtH RELEVANCe: ResoUrcEs, sUcH as mOney, Food, protection, OPporTunities, care, and emotionAl supPoRT thaT FlOw dirEctly frOM pERSon To PeRsoN aRE vItal fOr tHe well-bEing of chilDreN iN poor countriES tHat do not haVe sTrong market oR goVernmeNt InsTItutiOns. to undErstanD Why somE people thRiVE and

and public health. PUBLIC  HEALTH RE LEVAN CE:  Re so urce s, s uch as money,f ood,  protection, opportuni ties,  c a re,a nd  emot ional s u pp o r t t ha tflo wd ir ectly  fr om pers on to pers onar e vital fort he  well-bein g o f children i n p oor co un tri e s tha t d o not  haves trongmarket or  g o vernme n t insti t u ti ons.  To understand wh y  s o me people thri ve and

and_public health._PUBLIC HEALTH RELEVANCE: Resources,_such as_money,_food, protection,_opportunities,_care, and emotional_support that flow_directly from person to_person are vital_for_the well-being of children in poor countries that do not have strong market or_government_institutions. To_understand_why_some people thrive and


is discussed. Current protocols are described. With old patients the skin is examined, interim medical history discussed and current protocols are described. No biopsies are taken or blood drawn; other protocols cover these activities. If individuals enter a particular study the screening visit is attributed to that protocol. Some people may be potential candidates for a later

is discussed. Current protocols are described. With old patient the hide is examined, interim medical history discussed and current protocol are described. No biopsies are take or blood drawn; early protocol cover these activity. If individuals enter a finical sketch the screening sojourn is attributed to that protocol. Some citizenry may be likely candidates for a later

is discussed. Current protogols are describgd. With mld pafients tfe skin is examined, interim leeical history discussed and current irotocols are eescribed. Ik biopslzs ars takzn or blood drawk; other prodocols cover tvere activities. If individuals enter a [articukag study the scteenimd viapt is attributed to that protockl. Some people may br potential candidates for a lwter

is discussed. Current protocols are described. With the is examined, medical history discussed No are taken or drawn; other protocols these activities. If individuals enter a study the screening visit is attributed to that protocol. Some people may be candidates for a later

is discussed. Current protocoLs are descrIbed. WIth Old PaTienTs thE skin is examineD, InteRim medical history discuSsed aNd CUrreNT pRotocOls are dEScRIBed. no BiOpsIeS ArE takeN or Blood drAwn; other prOtoCoLs cover these ACtIvities. If iNdiViduals enter A paRticulAr StuDY the sCreEning Visit iS AttribUted to thaT pROtocol. sOme peopLE MaY be pOtential candidateS FoR A later

is discussed. Current pro tocols are  desc rib ed.  W itholdpatients the s k in i s examined, interim me dical  h i stor y  d iscus sed and cu r r ent  p ro toc ol s  a re de scr ibed. N o biopsies  ar etaken or blo o ddrawn; oth erprotocols co ver  these  a cti v ities . I f ind ividua l s ente r a parti cu l ar stu d y the s c r ee ning  visit is attribu t ed to that protoc ol. So me pe o p lemay  be potent ia l can d idatesf or a lat e r

is_discussed. Current_protocols are described. With_old patients_the_skin is_examined,_interim medical history_discussed and current_protocols are described. No_biopsies are taken_or_blood drawn; other protocols cover these activities. If individuals enter a particular study the_screening_visit is_attributed_to_that protocol. Some people may_be potential candidates for a_later