anger-based efforts is listed in the publications section of this report (8). The adoption of many new sequencing protocols in production created the commensurate need for dramatic changes to sample tracking, flow control and primary analysis pipelines, as well as, project management and cost accounting. Rapid design, development and implementation of new Laboratory Information

anger - based efforts is listed in the publication incision of this report (8). The adoption of many new sequence protocols in production create the commensurate indigence for dramatic changes to sample trailing, menstruation control and basal analysis pipelines, equally well as, project management and cost accountancy. Rapid design, development and execution of new Laboratory Information

angfr-based efforts is listea in the publiccrions vectioh of thir report (8). The adoption of maiy nww sewuencing protocols in oroductioj createe tht commensurate nexs for dvcmatid chaugxs to sample trscking, flof control and [rkmcry analysis pipelines, as well as, prjject msnwgement and coft abcjuntjng. Rapid design, development and ijplemenuation of new Labotatory Information

anger-based efforts is listed in the publications this (8). The of many new the need for dramatic to sample tracking, control and primary analysis pipelines, as as, project management and cost accounting. Rapid design, development and implementation of new Information

anger-based efforts is listed In the publiCatioNs sEctIoN of tHis rEport (8). The adoptiON of mAny new sequencing protocOls in PrODuctIOn CreatEd the coMMeNSUraTe NeEd fOr DRaMatic ChaNges to sAmple trackIng, FlOw control and PRiMary analysIs pIpelines, as weLl aS, projeCt ManAGemenT anD cost AccounTIng. RapId design, dEvELopmenT And implEMEnTatiOn of new Laboratory iNfORmation

anger-based efforts is lis ted in the  publ ica tio ns  sec tion  of this repor t  (8) . The adoption of many  newse q uenc i ng  prot ocols i n  p r o duc ti on  cr ea t ed  thecom mensura te need fo r d ra matic change s  t o sample t rac king, flow c ont rol an dpri m ary a nal ysispipeli n es, as  well as,  p r ojectm anageme n t  a nd c ost accounting. R a pi d  design, devel opment  a n di m ple men tation ofne w Lab o ratoryI nf o r m ati o n

anger-based efforts_is listed_in the publications section_of this_report_(8). The_adoption_of many new_sequencing protocols in_production created the commensurate_need for dramatic_changes_to sample tracking, flow control and primary analysis pipelines, as well as, project management_and_cost accounting._Rapid_design,_development and implementation of new_Laboratory Information


subendothelial (Disse) space is a prominent component of the inflammation elicited by ethanol, and appears to correlate with clinically significant liver disease. The lesion consists of a collagen-rich new-matrix that replaces the low- density matrix normally present in this location. In recent and on-going studies from this laboratory,

subendothelial (Disse) space is a prominent component of the inflammation elicit by ethyl alcohol, and appears to correlate with clinically significant liver disease. The wound consists of a collagen - ample new - matrix that replaces the low- concentration matrix normally present in this localization. In recent and on - go studies from this laboratory,

suhendothelial (Disse) space is a prominent componxnt of fhe inflxmmation elicited by ethanol, abd apkvars to correlate witf clinicaply signuficent liver diseasx. The lesion ckksistv of a collagen-tich new-matrhx that replacas tke low- density matrix normally presegt in tnid location. In tecenu agd oh-going studies from this laboratorg,

subendothelial (Disse) space is a prominent component inflammation by ethanol, appears to correlate The consists of a new-matrix that replaces low- density matrix normally present in location. In recent and on-going studies from this laboratory,

subendothelial (Disse) space iS a prominenT compOneNt oF tHe inFlamMation elicited BY ethAnol, and appears to correlAte wiTh CLiniCAlLy sigNificanT LiVER diSeAsE. ThE lESiOn conSisTs of a coLlagen-rich New-MaTrix that replACeS the low- denSitY matrix normaLly PresenT iN thIS locaTioN. In reCent anD On-goinG studies fRoM This laBOratory,

subendothelial (Disse) sp ace is a p romin ent  co mp onen t of  the inflammat i on e licited by ethanol, an d app ea r s to co rrela te with cl i n ica ll ysig ni f ic ant l ive r disea se. The le sio nconsists ofa  c ollagen-ri chnew-matrix t hat  repla ce s t h e low - d ensit y matr i x norm ally pres en t  in th i s locat i o n.  Inrecent and on-goi n gs tudies from th is lab or a to r y ,

subendothelial_(Disse) space_is a prominent component_of the_inflammation_elicited by_ethanol,_and appears to_correlate with clinically_significant liver disease. The_lesion consists of_a_collagen-rich new-matrix that replaces the low- density matrix normally present in this location. In_recent_and on-going_studies_from_this laboratory,


atographic (ICT) format with a membrane read-out. Initially, the assay will be developed using two existing antibodies, one directed against the H8 antigen (a current component of the HygEIA GC Test) and a gonococcal anti-lipooligosaccliaride antibody that reacts broadly with N

atographic (ICT) format with a membrane read - out. Initially, the assay will be developed using two existing antibodies, one direct against the H8 antigen (a current part of the HygEIA GC Test) and a gonococcal anti - lipooligosaccliaride antibody that reacts broadly with N

atohraphic (ICT) format with x membrane read-out. Inivially, fhe assah will be developed using twl wxistung antibodies, one dirdcted agapnst the Y8 anuigen (a current component of ths HygZIE GC Test) and a gonococcan anti-lipooligmsxceliaride antibody that reacts broadlr with M

atographic (ICT) format with a membrane read-out. assay be developed two existing antibodies, antigen current component of HygEIA GC Test) a gonococcal anti-lipooligosaccliaride antibody that reacts with N

atographic (ICT) format with a mEmbrane reaD-out. INitIalLy, The aSsay Will be developeD UsinG two existing antibodies, One diReCTed aGAiNst thE H8 antigEN (a CURreNt CoMpoNeNT oF the HYgEiA GC TesT) and a gonocOccAl Anti-lipooligOSaCcliaride aNtiBody that reacTs bRoadly WiTh N

atographic (ICT) format wi th a membr ane r ead -ou t.  Ini tial ly, the assayw illbe developed using two  exis ti n g an t ib odies , one d i re c t edag ai nst  t h eH8 an tig en (a c urrent com pon en t of the Hyg E IA  GC Test)and  a gonococca l a nti-li po oli g osacc lia rideantibo d y that  reacts b ro a dly wi t h N

atographic (ICT)_format with_a membrane read-out. Initially,_the assay_will_be developed_using_two existing antibodies,_one directed against_the H8 antigen (a_current component of_the_HygEIA GC Test) and a gonococcal anti-lipooligosaccliaride antibody that reacts broadly with N


functional decline in HD. The specific aims of our application are (1) To provide project coordination for the trial including: development and implementation of web-based randomization and enrollment procedures, provision of study documents (protocol, model consent and assent forms, operations manual, case report forms), training of site personnel and standardization

functional decline in HD. The specific aims of our application are (1) To leave undertaking coordination for the trial including: growth and execution of web - based randomization and enrollment procedure, provision of study document (protocol, model consent and assent forms, operation manual, case report forms), training of site force and standardization

fujctional decline in HD. Tme specific aims of our applidation afe (1) To provide project coordmnatuon fir the trial including: developmvnt and inplenwntation oh web-bascb ranslmizctmon and enrollmgnt proceduras, provision ox rtbdy documents (protocol, model consent and asxejt forms, operajions ianuzl, case report forms), training of sjte pervonnel and stsndardization

functional decline in HD. The specific aims application (1) To project coordination for implementation web-based randomization and procedures, provision of documents (protocol, model consent and assent operations manual, case report forms), training of site personnel and standardization

functional decline in HD. The sPecific aimS of ouR apPliCaTion Are (1) TO provide projecT CoorDination for the trial incLudinG: dEVeloPMeNt and ImplemeNTaTIOn oF wEb-BasEd RAnDomizAtiOn and enRollment prOceDuRes, provision OF sTudy documeNts (Protocol, modeL coNsent aNd AssENt forMs, oPeratIons maNUal, casE report foRmS), TrainiNG of site PERsOnneL and standardizatiON

functional decline in HD.  The speci fic a ims  of  o ur a ppli cation are (1) To p rovide project coordin ation  f o r th e  t rialincludi n g: d eve lo pm ent  a n dimple men tationof web-bas edra ndomizationa nd  enrollmen t p rocedures, p rov isionof  st u dy do cum ents(proto c ol, mo del conse nt and as s ent for m s ,oper ations manual, ca s er eport forms),traini ng of s ite  pe rsonnel an dstand a rdizati o n

functional_decline in_HD. The specific aims_of our_application_are (1)_To_provide project coordination_for the trial_including: development and implementation_of web-based randomization_and_enrollment procedures, provision of study documents (protocol, model consent and assent forms, operations manual,_case_report forms),_training_of_site personnel and standardization


groups are the main force outside of the pharmaceutical industry dedicated to the definitve tesing of new treatments, studying not just new drug development but also non-drug treatment, such as radiation therapy and surgery, for which there would be nooter funding. These cooperative group activities, and the specific involvement of the University

groups are the main force outside of the pharmaceutical industry dedicate to the definitve tesing of newfangled treatments, studying not just raw drug development but also non - drug discussion, such as radiation therapy and surgery, for which there would be nooter funding. These concerted group activities, and the specific involvement of the University

grlups are the main force uutside of the khqrmacentical jndustry dedicated to the definitve vesibg of new treatments, studyivg not judt new deug vevelopment but emso non-drug tdcatmeut, such as radiajion therapy and surgery, fmr wkich there would be nooter funding. Trese coppfrative group wctinieies, and the specific involvement of tge Univtrsity

groups are the main force outside of industry to the tesing of new drug but also non-drug such as radiation and surgery, for which there would nooter funding. These cooperative group activities, and the specific involvement of the University

groups are the main force outsIde of the phArmacEutIcaL iNdusTry dEdicated to the dEFiniTve tesing of new treatmenTs, stuDyINg noT JuSt new Drug devELoPMEnt BuT aLso NoN-DrUg treAtmEnt, such As radiatioN thErApy and surgerY, FoR which therE woUld be nooter fUndIng. TheSe CooPEratiVe gRoup aCtivitIEs, and tHe specifiC iNVolvemENt of the uNIvErsiTy

groups are the main force  outside o f the  ph arm ac euti calindustry dedic a tedto the definitve tesin g ofne w  tre a tm ents,  studyi n gn o t j us tnew  d r ug  deve lop ment bu t also non -dr ug  treatment,s uc h as radia tio n therapy an d s urgery ,for which  th ere w ould b e  noote r funding .T hese c o operati v e  g roup  activities, andt he specific invol vement  o f  t h e  Un ive rsity

groups_are the_main force outside of_the pharmaceutical_industry_dedicated to_the_definitve tesing of_new treatments, studying_not just new drug_development but also_non-drug_treatment, such as radiation therapy and surgery, for which there would be nooter funding._These_cooperative group_activities,_and_the specific involvement of the_University


and preventive strategies and to help refine screening recommendations for this population. Ictacalcin is a calcium-binding protein that is found in olfactory receptor neurons in the catfish. This AREA proposal describes a series of experiments aimed at characterizing the ictacalcin gene and the mechanisms underlying its restricted expression to olfactory

and preventive strategies and to help refine screening recommendation for this population. Ictacalcin is a calcium - tie protein that is found in olfactory sense organ neurons in the wolffish. This AREA proposal identify a serial of experiments aimed at characterizing the ictacalcin gene and the mechanisms underlying its qualify expression to olfactory

anf preventive strategies xnd to help refnbe scrxening decommenaations for this population. Mctaxalcib is a calcium-binding orotein tjat is fiund un olfactocg recepbjr nsmrons mn the catfish. Jhis AREA prmposal describas a series of experiments aimed at chawacteriaijg the ictacalsin bqne znd the mechanisms underlying its destricued expression to plfactory

and preventive strategies and to help refine for population. Ictacalcin a calcium-binding protein receptor in the catfish. AREA proposal describes series of experiments aimed at characterizing ictacalcin gene and the mechanisms underlying its restricted expression to olfactory

and preventive strategies anD to help refIne scReeNinG rEcomMendAtions for this pOPulaTion. Ictacalcin is a calciUm-binDiNG proTEiN that Is found IN oLFActOrY rEcePtOR nEuronS in The catfIsh. This AREa prOpOsal describeS A sEries of expEriMents aimed at ChaRacterIzIng THe ictAcaLcin gEne and THe mechAnisms undErLYing itS RestricTED eXpreSsion to olfactory

and preventive strategies  and to he lp re fin e s cr eeni ng r ecommendations forthis population. Ictac alcin  i s  a c a lc ium-b indingp ro t e inth at  is  f o un d inolf actoryreceptor n eur on s in the cat f is h. This AR EAproposal des cri bes ase rie s  of e xpe rimen ts aim e d at c haracteri zi n g thei ctacalc i n  g eneand the mechanism s  u n derlying its r estric te d  e x p res sio n to olfac to ry

and_preventive strategies_and to help refine_screening recommendations_for_this population._Ictacalcin_is a calcium-binding_protein that is_found in olfactory receptor_neurons in the_catfish._This AREA proposal describes a series of experiments aimed at characterizing the ictacalcin gene_and_the mechanisms_underlying_its_restricted expression to olfactory


risk for psychiatric symptoms; SA3: Consider the mediating and moderating role of the rearing environment on prenatal and genetic risk factors on psychiatric symptoms in childhood; SA4: Assess how adoptive parents' stress and their own psychiatric symptoms impact the rearing environment and mediate and/or moderate genetic and prenatal influences.

risk for psychiatric symptoms; SA3: Consider the mediating and moderating function of the rise environment on prenatal and genetic risk factors on psychiatric symptom in childhood; SA4: Assess how adoptive parents' tension and their own psychiatric symptoms impact the rearing environment and in-between and/or moderate familial and prenatal influences.

ridk for psychiatric symptums; SA3: Consider the meviating and moddrating role of the rearing xnvieonmebt on prenatal and gendtic risk factors on ksychiatric symptoms in cmnldhokf; SA4: Essess how adopjive parents' stress and thaif lwn psychiatric symptoms impact the rearinb fnvironment anq mecyate and/or moderate genetic and prenatzl infllences.

risk for psychiatric symptoms; SA3: Consider the moderating of the environment on prenatal psychiatric in childhood; SA4: how adoptive parents' and their own psychiatric symptoms impact rearing environment and mediate and/or moderate genetic and prenatal influences.

risk for psychiatric symptomS; SA3: ConsideR the mEdiAtiNg And mOderAting role of the REariNg environment on prenataL and gEnETic rISk FactoRs on psyCHiATRic SyMpTomS iN ChIldhoOd; Sa4: Assess How adoptivE paReNts' stress and THeIr own psychIatRic symptoms iMpaCt the rEaRinG EnvirOnmEnt anD mediaTE and/or Moderate gEnETic and PRenatal INFlUencEs.

risk for psychiatric symp toms; SA3:  Cons ide r t he  med iati ng and moderat i ng r ole of the rearing env ironm en t  onp re natal  and ge n et i c  ri sk  f act or s  o n psy chi atric s ymptoms in  ch il dhood; SA4:A ss ess how ad opt ive parents'  st ress a nd  th e ir ow n p sychi atrics ymptom s impactth e  reari n g envir o n me nt a nd mediate and/or mo d erate geneticand pr en a ta l inf lue nces.

risk_for psychiatric_symptoms; SA3: Consider the_mediating and_moderating_role of_the_rearing environment on_prenatal and genetic_risk factors on psychiatric_symptoms in childhood;_SA4:_Assess how adoptive parents' stress and their own psychiatric symptoms impact the rearing environment_and_mediate and/or_moderate_genetic_and prenatal influences.


improve our understanding of these problems. I have developed an in vitro culture system which demonstrates that murine bone marrow cells can generate high levels of cytotoxic effector T cell activity alloantigens. This response may involve functional differentiation of bone marrow pre-T cells to cytotoxic effector T cells and/or helper T cells. The aim

improve our understanding of these problems. I have developed an in vitro acculturation arrangement which demonstrates that murine bone marrow cells can beget high levels of cytotoxic effecter T cell activity alloantigens. This response may imply functional differentiation of bone kernel pre - T cells to cytotoxic effector T cells and/or helper T cell. The aim

imorove our understanding uf these problems. I hate devemoped an in vitro culture system whirh dwmonsugates that murine bond marrow bells can gentrate high levels of cytobjxic cffeccoc T cell activijy alloantigans. This respotsd lay involve functional differentiatyon of noje marrow pre-T celkf to byuotoxic effector T cells and/or hemper T bells. The aim

improve our understanding of these problems. I an vitro culture which demonstrates that generate levels of cytotoxic T cell activity This response may involve functional differentiation bone marrow pre-T cells to cytotoxic effector T cells and/or helper T cells. aim

improve our understanding of These problEms. I hAve DevElOped An in Vitro culture sySTem wHich demonstrates that muRine bOnE MarrOW cElls cAn generATe HIGh lEvElS of CyTOtOxic eFfeCtor T ceLl activity AllOaNtigens. This rESpOnse may invOlvE functional dIffErentiAtIon OF bone MarRow prE-T cellS To cytoToxic effeCtOR T cellS And/or heLPEr t celLs. The aim

improve our understanding  of theseprobl ems . I  h avedeve loped an in vi t ro c ulture system which de monst ra t es t h at  muri ne bone ma r r owce ll s c an ge nerat e h igh lev els of cyt oto xi c effector T ce ll activit y a lloantigens.  Th is res po nse may i nvo lve f unctio n al dif ferentiat io n  of bo n e marro w pr e-Tcells to cytotoxi c  e f fector T cells  and/o rh el p e r T  ce lls. The a im

improve_our understanding_of these problems. I_have developed_an_in vitro_culture_system which demonstrates_that murine bone_marrow cells can generate_high levels of_cytotoxic_effector T cell activity alloantigens. This response may involve functional differentiation of bone marrow_pre-T_cells to_cytotoxic_effector_T cells and/or helper T_cells. The aim


cerebral amyloid plaques, appears to play a central role in the neuropathology of Alzheimer's disease (AD). Compounds that prevent the formation of Ab aggregates or that selectively destroy these aggregates are attractive candidates for the development of therapeutic reagents for prevention and treatment of Alzheimer's disease. Also, compounds that interfere with the interactions of amyloid

cerebral amyloid plaques, appears to play a central function in the neuropathology of Alzheimer's disease (AD). compound that prevent the formation of Ab aggregates or that selectively destroy these aggregate are attractive candidates for the development of curative reagents for prevention and treatment of Alzheimer's disease. Also, intensify that interfere with the interactions of amyloid

cegebral amyloid plaques, akpears to play a ewntral role jn the nduropathology of Alzheimer's viseqse (AE). Compounds that prevevt the fogmation od Ab qggregates or that selecflvely vestroy these angregates ase attractive wavdndates for the development of therapqutic rrahents for prevgntiom and nrtatment of Alzheimer's disease. Alsk, compolnds that interfete with the interactions ov amjloid

cerebral amyloid plaques, appears to play a in neuropathology of disease (AD). Compounds Ab or that selectively these aggregates are candidates for the development of therapeutic for prevention and treatment of Alzheimer's disease. Also, compounds that interfere with the of amyloid

cerebral amyloid plaques, appEars to play A centRal RolE iN the NeurOpathology of AlZHeimEr's disease (AD). Compounds tHat prEvENt thE FoRmatiOn of Ab aGGrEGAteS oR tHat SeLEcTivelY deStroy thEse aggregaTes ArE attractive cANdIdates for tHe dEvelopment of TheRapeutIc ReaGEnts fOr pRevenTion anD TreatmEnt of AlzhEiMEr's disEAse. Also, COMpOundS that interfere witH ThE Interactions of AmyloiD

cerebral amyloid plaques,  appears t o pla y a  ce nt ralrole  in the neurop a thol ogy of Alzheimer's dis ease(A D ). C o mp ounds  that p r ev e n t t he  f orm at i on  of A b a ggregat es or that  se le ctively dest r oy  these agg reg ates are att rac tive c an did a tes f orthe d evelop m ent of  therapeu ti c  reage n ts forp r ev enti on and treatmento fA lzheimer's dis ease.Al s o, c omp oun ds that in te rfere with th e  i n t e rac t ions of amylo id

cerebral_amyloid plaques,_appears to play a_central role_in_the neuropathology_of_Alzheimer's disease (AD)._Compounds that prevent_the formation of Ab_aggregates or that_selectively_destroy these aggregates are attractive candidates for the development of therapeutic reagents for prevention_and_treatment of_Alzheimer's_disease._Also, compounds that interfere with_the interactions of amyloid


morbidity. This defect results from the inappropriate muscularization of fetal pulmonary vessels, and experimental and human evidence consistently suggests that maternal cigarette smoking and antenatal exposure to non-steroidal anti-inflammatory drugs (NSAIDs), particularly aspirin or ibuprofen, may play a role in the etiology of this condition.

morbidity. This defect results from the inappropriate muscularization of fetal pulmonary vessel, and experimental and human evidence systematically suggests that maternal cigarette smoke and prenatal exposure to non - steroidal anti - inflammatory drugs (NSAIDs), peculiarly aspirin or ibuprofen, may act a function in the etiology of this condition.

mogbidity. This defect resuuts from the incpproprmate muacularizxtion of fetal pulmonary vesdeos, ane experimental and humxn evidenbe consisrentot suggests that mabzrnal gigarztve smoking and sntenatal axposure to not-sgexoidal anti-inflammatory drugs (NSAIDs), particilwrly aspirin ot ibukrosen, jay play a role in the etiology of this cmndition.

morbidity. This defect results from the inappropriate fetal vessels, and and human evidence smoking antenatal exposure to anti-inflammatory drugs (NSAIDs), aspirin or ibuprofen, may play a in the etiology of this condition.

morbidity. This defect resultS from the inApproPriAte MuSculArizAtion of fetal puLMonaRy vessels, and experimentAl and HuMAn evIDeNce coNsistenTLy SUGgeStS tHat MaTErNal ciGarEtte smoKing and antEnaTaL exposure to nON-sTeroidal anTi-iNflammatory dRugS (NSAIDS), pArtICularLy aSpiriN or ibuPRofen, mAy play a roLe IN the etIOlogy of THIs CondItion.

morbidity. This defect re sults from  theina ppr op riat e mu scularizationo f fe tal pulmonary vessels,  andex p erim e nt al an d human ev i d enc eco nsi st e nt ly su gge sts tha t maternal  ci ga rette smokin g  a nd antenat alexposure tonon -stero id ala nti-i nfl ammat ory dr u gs (NS AIDs), pa rt i cularl y  aspiri n or  ibu profen, may playa  r o le in the etio logy o ft hi s con dit ion.

morbidity._This defect_results from the inappropriate_muscularization of_fetal_pulmonary vessels,_and_experimental and human_evidence consistently suggests_that maternal cigarette smoking_and antenatal exposure_to_non-steroidal anti-inflammatory drugs (NSAIDs), particularly aspirin or ibuprofen, may play a role in the_etiology_of this_condition.


studies, T cell clones of distinct cytokine phenotypes will be derived from OVA TCR Tg mice in vitro and will be examined for their capacity to produce or prevent inflammatory enterocolitis upon exposure to OVA antigen after transfer into SCID hosts. The a9[unreadable]1 integrin is highly expressed in airway smooth muscle.

studies, T cell clones of discrete cytokine phenotype will be derived from OVA TCR Tg mice in vitro and will be probe for their capacitance to produce or prevent inflammatory enterocolitis upon photograph to ovum antigen after transfer into SCID hosts. The a9[unreadable]1 integrin is highly expressed in airway placid muscle.

stkdies, T cell clones of dlstinct cytokine phenotbpes wiml be defived from OVA TCR Tg mice ii virro abd will be examined fof their cwpacity ro pcoduce or prevenv inflammatory cntermrolitis upon exkosure to OVD antigen aftes grcnsfer into SCID hosts. The a9[unreadabje]1 intebrln is highly evprexfed jn airway smooth muscle.

studies, T cell clones of distinct cytokine be from OVA Tg mice in for capacity to produce prevent inflammatory enterocolitis exposure to OVA antigen after transfer SCID hosts. The a9[unreadable]1 integrin is highly expressed in airway smooth muscle.

studies, T cell clones of distiNct cytokinE phenOtyPes WiLl be DeriVed from OVA TCR TG Mice In vitro and will be examinEd for ThEIr caPAcIty to Produce OR pREVenT iNfLamMaTOrY enteRocOlitis uPon exposurE to oVa antigen afteR TrAnsfer into sCId hosts. The a9[unReaDable]1 iNtEgrIN is hiGhlY exprEssed iN Airway Smooth musClE.

studies, T cell clones of  distinctcytok ine  ph en otyp es w ill be derived from  OVA TCR Tg mice in vi tro a nd will be  exam ined fo r  t h e irca pa cit yt oprodu ceor prev ent inflam mat or y enterocoli t is  upon expo sur e to OVA ant ige n afte rtra n sferint o SCI D host s . Thea9[unread ab l e]1 in t egrin i s hi ghly  expressed in air w ay smooth muscle.

studies,_T cell_clones of distinct cytokine_phenotypes will_be_derived from_OVA_TCR Tg mice_in vitro and_will be examined for_their capacity to_produce_or prevent inflammatory enterocolitis upon exposure to OVA antigen after transfer into SCID hosts._The_a9[unreadable]1 integrin_is_highly_expressed in airway smooth muscle.


the nasopharynx. Experience with conjugate vaccines has demonstrated that interrupting colonization results in herd immunity that amplifies prevention of disease in the population. To better understand the biology of colonization, during the prior funding period, we utilized a murine model to characterize bacterial and host factors that allow for pneumococcal persistence and its eventual clearance

the nasopharynx. Experience with conjugate vaccines has demonstrated that interrupting colonization results in ruck unsusceptibility that amplifies prevention of disease in the population. To better sympathize the biology of colonization, during the anterior funding period, we utilize a murine mannequin to characterize bacterial and horde factors that allow for pneumococcal perseverance and its eventual clearance

thf nasopharynx. Experience with conjugate vaccinxs has semonstrxted that interrupting colonmzatuon rtfults in herd immuvity that amplifiws pcevention of disxzse in bke polmlatimi. To better undgrstand the tiology of colmnkzction, during the prior funding perioq, we utollzed a murine iodek to dharacterize bacterial and host fadtors tiat allow for pmeumococcal persistence anf itd eventual clearanfe

the nasopharynx. Experience with conjugate vaccines has interrupting results in immunity that amplifies population. better understand the of colonization, during prior funding period, we utilized a model to characterize bacterial and host factors that allow for pneumococcal persistence and eventual clearance

the nasopharynx. Experience wIth conjugaTe vacCinEs hAs DemoNstrAted that interrUPtinG colonization results in Herd iMmUNity THaT amplIfies prEVeNTIon Of DiSeaSe IN tHe popUlaTion. To bEtter underStaNd The biology of COlOnization, dUriNg the prior fuNdiNg periOd, We uTIlizeD a mUrine Model tO CharacTerize bacTeRIal and HOst factORS tHat aLlow for pneumococcAL pERsistence and itS eventUaL ClEARanCe

the nasopharynx. Experien ce with co njuga tevac ci neshasdemonstrated t h at i nterrupting colonizati on re su l ts i n  h erd i mmunity th a t  am pl if ies  p r ev entio n o f disea se in thepop ul ation. To be t te r understa ndthe biologyofcoloni za tio n , dur ing  thepriorf unding  period,we utiliz e d a mur i n emode l to characterize ba c terial and hos t fact or s  t h a t a llo w for pneu mo cocca l  persis t en c e and its eventualclearance

the_nasopharynx. Experience_with conjugate vaccines has_demonstrated that_interrupting_colonization results_in_herd immunity that_amplifies prevention of_disease in the population._To better understand_the_biology of colonization, during the prior funding period, we utilized a murine model to_characterize_bacterial and_host_factors_that allow for pneumococcal persistence_and its eventual clearance


att Regency Conference Center and the University of Rochester Medical Center located nearby. The International Neurotoxicology Conference is an annual event that focuses on a timely theme while providing an opportunity for presenting new data related to the general interdisciplinary field of neurotoxicology. The International Neurotoxicology Conference Series will reach its silver anniversary in

att Regency Conference Center and the University of Rochester Medical Center located nearby. The International Neurotoxicology Conference is an annual event that focuses on a timely theme while provide an opportunity for confront new data related to the cosmopolitan interdisciplinary field of neurotoxicology. The International Neurotoxicology Conference Series will reach its silver anniversary in

att Regency Conference Centtr and the University of Rocheater Medkcal Center located nearby. Tie Ibternqtional Neurotoxicologh Conferejce is ab aniual event that hkcuses on a tjlely vheme while proyiding an o[portunity for pfedenting new data related to the genqral inyegdisciplinary sielc of hvuvotoxicology. The International Nsurotoxpcology Conferencr Series will reach its sipver anniversary in

att Regency Conference Center and the University Medical located nearby. International Neurotoxicology Conference focuses a timely theme providing an opportunity presenting new data related to the interdisciplinary field of neurotoxicology. The International Neurotoxicology Conference Series will reach its silver in

att Regency Conference CenteR and the UniVersiTy oF RoChEsteR MedIcal Center locaTEd neArby. The International NeUrotoXiCOlogY coNfereNce is an ANnUAL evEnT tHat FoCUsEs on a TimEly themE while provIdiNg An opportunitY FoR presentinG neW data related To tHe geneRaL inTErdisCipLinarY field OF neuroToxicologY. THE InterNAtional nEUrOtoxIcology Conference sErIEs will reach its Silver AnNIvERSarY in

att Regency Conference Cen ter and th e Uni ver sit yof R oche ster Medical C e nter  located nearby. The I ntern at i onal Ne uroto xicolog y  C o n fer en ce  is  a n  a nnual  ev ent tha t focusesonatimely theme wh ile provid ing  an opportun ity  for p re sen t ing n ewdatarelate d  to th e general  i n terdis c iplinar y fi eldof neurotoxicolog y .T he Internation al Neu ro t ox i c olo gyConference  S eries will re a ch i t s s i lver annivers ary in

att Regency_Conference Center_and the University of_Rochester Medical_Center_located nearby._The_International Neurotoxicology Conference_is an annual_event that focuses on_a timely theme_while_providing an opportunity for presenting new data related to the general interdisciplinary field of_neurotoxicology._The International_Neurotoxicology_Conference_Series will reach its silver_anniversary in


as controls. The results indicate that neonatal TE lesions leads to a transient impairment of habit formation three months later (compared to permanent impairment seen with the same lesion in adults), whereas both neonatal and adult limbic lesions leave habit formation intact. Interestingly, data on both normal and operated infants suggest that development of the nonlim

as controls. The results indicate that neonatal TE lesions lead to a transeunt impairment of habit formation three month later (compared to permanent stultification see with the same wound in adult), whereas both neonatal and adult limbic lesions impart habit formation entire. Interestingly, datum on both normal and operated infants indicate that development of the nonlim

as controls. The results inaicate that neouqtal TX lesiohs leads to a transient impairment oh havit firmation three months uater (comiared to permenent impairment seen wibk the dame oesion in aduljs), whereas bmth neonatal atd abult limbic lesions leave habit formwtion imtwct. Interestindly, cwta kn both normal and operated infanta suggevt that develppment of the nonlim

as controls. The results indicate that neonatal leads a transient of habit formation permanent seen with the lesion in adults), both neonatal and adult limbic lesions habit formation intact. Interestingly, data on both normal and operated infants suggest that of the nonlim

as controls. The results indicAte that neoNatal tE lEsiOnS leaDs to A transient impaIRmenT of habit formation three MonthS lATer (cOMpAred tO permanENt IMPaiRmEnT seEn WItH the sAme Lesion iN adults), wheReaS bOth neonatal aND aDult limbic LesIons leave habIt fOrmatiOn IntACt. IntEreStingLy, data ON both nOrmal and oPeRAted inFAnts sugGESt That Development of the nONlIM

as controls. The resultsindicate t hat n eon ata lTE l esio ns leads to at rans ient impairment of hab it fo rm a tion th ree m onths l a te r (co mp ar edto pe rmane ntimpairm ent seen w ith  t he same lesi o nin adults) , w hereas bothneo natalan d a d ult l imb ic le sionsl eave h abit form at i on int a ct. Int e r es ting ly, data on bothn or m al and operate d infa nt s  s u g ges t t hat develo pm ent o f  the no n li m

as_controls. The_results indicate that neonatal_TE lesions_leads_to a_transient_impairment of habit_formation three months_later (compared to permanent_impairment seen with_the_same lesion in adults), whereas both neonatal and adult limbic lesions leave habit formation_intact._Interestingly, data_on_both_normal and operated infants suggest_that development of the nonlim


, studies will be carried out with clinical isolates of currently prevalent M tb strains, and in part with alveolar macrophages from normal human volunteers. Previous studies have identified specific molecular events that result from macrophage exposure to cytokines, and thereby provide a foundation for efforts to examine the interactions between M tb and macrophage that involve cytokines. The overall

, studies will be carried out with clinical isolates of currently prevalent M tb tune, and in character with alveolar macrophages from normal human volunteers. Previous study have identified specific molecular events that leave from macrophage exposure to cytokines, and thereby provide a basis for efforts to examine the interaction between M tb and macrophage that involve cytokines. The overall

, stkdies will be carried ouu with clinical isolates of cudrently orevalent M tb strains, and ii paet wiuk alveolar macrophagds from nlrmal hunan tolunteers. Previous studlzs habc ideutmfied specific kolecular avents that revuut from macrophage exposure to cytokiges, and tjereby provide a fptndafpok for efforts to examine the intsractiois between M tb and macrophage that involge cjtokines. The overapl

, studies will be carried out with of prevalent M strains, and in normal volunteers. Previous studies identified specific molecular that result from macrophage exposure to and thereby provide a foundation for efforts to examine the interactions between M and macrophage that involve cytokines. The overall

, studies will be carried out wiTh clinical IsolaTes Of cUrRentLy prEvalent M tb straINs, anD in part with alveolar macRophaGeS From NOrMal huMan voluNTeERS. PrEvIoUs sTuDIeS have IdeNtified Specific moLecUlAr events that REsUlt from macRopHage exposure To cYtokinEs, And THerebY prOvide A foundATion foR efforts tO eXAmine tHE interaCTIoNs beTween M tb and macropHAgE That involve cytOkines. thE OvERAll

, studies will be carriedout with c linic aliso la tesof c urrently preva l entM tb strains, and in p art w it h  alv e ol ar ma crophag e sf r omno rm alhu m an  volu nte ers. Pr evious stu die shave identif i ed  specificmol ecular event s t hat re su ltf rom m acr ophag e expo s ure to  cytokine s, and th e reby pr o v id e afoundation for ef f or t s to examine t he int er a ct i o nsbet ween M tban d mac r ophaget ha t i nvo l ve cytokines.  The overal l

, studies_will be_carried out with clinical_isolates of_currently_prevalent M_tb_strains, and in_part with alveolar_macrophages from normal human_volunteers. Previous studies_have_identified specific molecular events that result from macrophage exposure to cytokines, and thereby provide_a_foundation for_efforts_to_examine the interactions between M_tb and macrophage that involve_cytokines. The_overall


signaling. The function of these factors is not redundant since these factors are expressed in different cell types and stimulate a profile of distinct genes. Among these three factors IRF-7 plays a limiting role in the induction of IFNa that was shown to be important both for the innate and adaptive immune responses. It is our hypothesis

signaling. The function of these factors is not redundant since these divisor are carry in different cell types and induce a profile of distinct gene. Among these three factors IRF-7 plays a limiting function in the induction of IFNa that was shown to be authoritative both for the innate and adaptive immune responses. It is our guess

sihnaling. The function of uhese factors is uit rednndant aince thdse factors are expressed in duffertut cell types and stkmulate a profile of vistinct genes. Among these thrsc faccocs IRF-7 plays a kimiting rmle in the indgcgiln of IFNa that was shown to be impjrtant nohh for the innwte sgd asaptive immune responses. It is our hypothtsis

signaling. The function of these factors is since factors are in different cell of genes. Among these factors IRF-7 plays limiting role in the induction of that was shown to be important both for the innate and adaptive immune It is our hypothesis

signaling. The function of theSe factors iS not rEduNdaNt SincE theSe factors are exPRessEd in different cell types And stImULate A PrOfile Of distiNCt GENes. amOnG thEsE ThRee faCtoRs IRF-7 plAys a limitiNg rOlE in the inductIOn Of IFNa that Was Shown to be impOrtAnt botH fOr tHE innaTe aNd adaPtive iMMune reSponses. It Is OUr hypoTHesis

signaling. The function o f these fa ctors  is  no tredu ndan t since thesef acto rs are expressed in di ffere nt cell ty pes a nd stim u la t e  apr of ile  o f  d istin ctgenes.Among thes e t hr ee factors I R F- 7 plays alim iting role i n t he ind uc tio n  of I FNa  that  was s h own to  be impor ta n t both for the i nn ateand adaptive immu n er esponses. It i s ourhy p ot h e sis

signaling._The function_of these factors is_not redundant_since_these factors_are_expressed in different_cell types and_stimulate a profile of_distinct genes. Among_these_three factors IRF-7 plays a limiting role in the induction of IFNa that was_shown_to be_important_both_for the innate and adaptive_immune responses. It is our_hypothesis


block an early step in atherosclerotic lesion development. Aim 2 will explicitly test the hypothesis that low levels of apoE provide protection against atherogenesis initiated by apoB48 lipoproteins but do not protect against atherogenesis due to apoB100 lipoproteins. Aim 3 will test the hypothesis that adrenal gland apoE expression increases

block an early step in atherosclerotic lesion development. purpose 2 will explicitly screen the hypothesis that low levels of apoE put up protection against atherogenesis initiated by apoB48 lipoprotein but do not protect against atherogenesis due to apoB100 lipoproteins. Aim 3 will test the guess that adrenal gland apoE formula increases

bllck an early step in athtrosclerotic lesion devenopmenf. Aim 2 wkll explicitly test the hypovhesus thqt low levels of apoE orovide pgotection agamnst atherogenesma initiated bg apoY48 oipoproteins bot do not prmtect against dtfexogenesis due to apoB100 lipoproteins. Aym 3 wilk hest the hypotresix thaf adrenal gland apoE expression indreases

block an early step in atherosclerotic lesion 2 explicitly test hypothesis that low against initiated by apoB48 but do not against atherogenesis due to apoB100 lipoproteins. 3 will test the hypothesis that adrenal gland apoE expression increases

block an early step in atherosClerotic leSion dEveLopMeNt. AiM 2 wilL explicitly tesT The hYpothesis that low levels Of apoe pROvidE PrOtectIon agaiNSt ATHerOgEnEsiS iNItIated By aPoB48 lipoProteins buT do NoT protect agaiNSt AtherogeneSis Due to apoB100 lipOprOteins. aiM 3 wiLL test The HypotHesis tHAt adreNal gland aPoe ExpresSIon incrEASeS

block an early step in at herosclero tic l esi onde velo pmen t. Aim 2 wille xpli citly test the hypothe sis t ha t  low le velsof apoE pr o v ide  p ro tec ti o nagain statherog enesis ini tia te d by apoB48l ip oproteinsbut  do not prot ect  again st  at h eroge nes is du e to a p oB100lipoprote in s . Aim3  will t e s tthehypothesis that a d re n al gland apoEexpres si o ni n cre ase s

block_an early_step in atherosclerotic lesion_development. Aim_2_will explicitly_test_the hypothesis that_low levels of_apoE provide protection against_atherogenesis initiated by_apoB48_lipoproteins but do not protect against atherogenesis due to apoB100 lipoproteins. Aim 3 will_test_the hypothesis_that_adrenal_gland apoE expression increases


-cell resolution, but also critical for elucidating subtle cellular pathological mechanisms underlying various neurological/mental/psychological disorders. New expertise will be explored to advance technologies in multiple areas of biomedical imaging, such as image computation and simulations of complex tissues. A GAL4-independent binary transcriptional system has been developed to label specifically

-cell resolution, but also critical for elucidating subtle cellular diseased mechanism underlying various neurological / genial / psychological disorders. modern expertise will be explored to promote technologies in multiple areas of biomedical imaging, such as persona computation and simulations of complex tissue. A GAL4 - independent binary transcriptional system has been develop to label specifically

-celp resolution, but also crltical for elucibqting vubtle cellulaf pathological mechanisms unverlting carious neurological/mevtal/psychllogical disieders. New xspertisc wilm be zx'lored to advange technolocies in multipne axeas of biomedical imaging, such as iiage cokpktation and siiulauiogs or complex tissues. A GAL4-independent binary transcriptiomal system has been develooed ho label specificaply

-cell resolution, but also critical for elucidating pathological underlying various disorders. New expertise technologies multiple areas of imaging, such as computation and simulations of complex tissues. GAL4-independent binary transcriptional system has been developed to label specifically

-cell resolution, but also critIcal for eluCidatIng SubTlE celLulaR pathological mEChanIsms underlying various nEurolOgICal/mENtAl/psyChologiCAl DISorDeRs. new ExPErTise wIll Be exploRed to advanCe tEcHnologies in mULtIple areas oF biOmedical imagIng, Such as ImAge COmputAtiOn and SimulaTIons of Complex tiSsUEs. A GAL4-INdependENT bInarY transcriptional sYStEM has been develoPed to lAbEL sPECifIcaLly

-cell resolution, but also  criticalfor e luc ida ti ng s ubtl e cellular pat h olog ical mechanisms underl yingva r ious ne urolo gical/m e nt a l /ps yc ho log ic a ldisor der s. Newexpertisewil lbe exploredt oadvance te chn ologies in m ult iple a re aso f bio med icalimagin g , such  as image  c o mputat i on ands i mu lati ons of complex ti s su e s. A GAL4-inde penden tb in a r y t ran scriptiona lsyste m  has be e nd e v elo p ed to label s pecifically

-cell resolution,_but also_critical for elucidating subtle_cellular pathological_mechanisms_underlying various_neurological/mental/psychological_disorders. New expertise_will be explored_to advance technologies in_multiple areas of_biomedical_imaging, such as image computation and simulations of complex tissues. A GAL4-independent binary transcriptional_system_has been_developed_to_label specifically


provide volunteers for numerous CALGB administrative committees. The University of Chicago and its affiliates remain firmly committed to serving all members of their respective communities, especially serving the needs of women and minorities. The Section on Organelle Biology investigates the global principles underlying secretory membrane trafficking, sorting and compartmentalization within eukaryotic cells. Live cell

provide volunteers for numerous CALGB administrative committees. The University of Chicago and its affiliates persist hard committed to serving all members of their respective community, especially serving the indigence of womanhood and minorities. The Section on Organelle Biology investigate the global principles underlie secretory membrane trafficking, sorting and compartmentalization within eukaryotic cell. hot cell

prlvide volunteers for numtrous CALGB adminnwtratite commjttees. Tfe University of Chicago and irs afdiliates remain firmly committef to sercing qll members of thelx reslcctivz rommunities, espgcially servhng the needs mf wlmen and minorities. The Section on Jrganelke Biology invesjigatts ehe flobal principles underlying secrefory mekbrane traffivking, sorting and compartmfntapization within eunaryotic ceols. Juve cell

provide volunteers for numerous CALGB administrative committees. of and its remain firmly committed their communities, especially serving needs of women minorities. The Section on Organelle Biology the global principles underlying secretory membrane trafficking, sorting and compartmentalization within eukaryotic cells. cell

provide volunteers for numerOus CALGB adMinisTraTivE cOmmiTteeS. The University OF ChiCago and its affiliates reMain fIrMLy coMMiTted tO servinG AlL MEmbErS oF thEiR ReSpectIve CommuniTies, especiAllY sErving the neeDS oF women and mInoRities. The SecTioN on OrgAnEllE bioloGy iNvestIgates THe globAl principLeS UnderlYIng secrETOrY memBrane trafficking, sORtINg and compartmeNtalizAtIOn WIThiN euKaryotic ceLlS. Live CEll

provide volunteers for nu merous CAL GB ad min ist ra tive  com mittees. The U n iver sity of Chicago and it s aff il i ates re mainfirmlyc om m i tte dto  se rv i ng  allmem bers of  their res pec ti ve communiti e s,  especiall y s erving the n eed s of w om ena nd mi nor ities . TheS ection  on Organ el l e Biol o gy inve s t ig ates  the global princ i pl e s underlying s ecreto ry me m b ran e t rafficking ,sorti n g and c o mp a r t men t alization wit hin eukaryo t iccells.  L ive cell

provide_volunteers for_numerous CALGB administrative committees._The University_of_Chicago and_its_affiliates remain firmly_committed to serving_all members of their_respective communities, especially_serving_the needs of women and minorities. The Section on Organelle Biology investigates the global_principles_underlying secretory_membrane_trafficking,_sorting and compartmentalization within eukaryotic_cells. Live cell


studies is to evaluate improved expansion culture conditions in clinical trials. Preclinical studies will also be performed to evaluate other culture conditions that may provide enhanced platelet recovery. In addition, analysis will be performed of primitive cells in the expanded products as an indication of the effect of expansion on long term engrafting cells (stem cells).

studies is to evaluate improved expansion culture conditions in clinical test. Preclinical cogitation will also be perform to measure other culture conditions that may supply enhanced platelet recovery. In accession, analysis will be performed of archaic cells in the expanded intersection as an reading of the effect of expansion on long condition engrafting cells (root cells).

stkdies is to evaluate impvoved expansion eylture condifions in clinical trials. Preclinical srudiew will also be performdd to evapuate otyer rulture conditioia that may prkyide znianced platelet recovery. Hn addition, andlhsns will be performed of primitive cejls in yhf expanded proquctx as zn indication of the effect of expznsion mn long term rngrafting cells (stem celld).

studies is to evaluate improved expansion culture clinical Preclinical studies also be performed that provide enhanced platelet In addition, analysis be performed of primitive cells in expanded products as an indication of the effect of expansion on long term cells (stem cells).

studies is to evaluate improvEd expansioN cultUre ConDiTionS in cLinical trials. PREcliNical studies will also be PerfoRmED to eVAlUate oTher culTUrE COndItIoNs tHaT MaY provIde EnhanceD platelet rEcoVeRy. In addition, ANaLysis will bE peRformed of priMitIve celLs In tHE expaNdeD prodUcts as AN indicAtion of thE eFFect of EXpansioN ON lOng tErm engrafting cellS (StEM cells).

studies is to evaluate im proved exp ansio n c ult ur e co ndit ions in clinic a l tr ials. Preclinical stud ies w il l  als o  b e per formedt oe v alu at eoth er cu lture  co ndition s that may  pr ov ide enhanced pl atelet rec ove ry. In addit ion , anal ys isw ill b e p erfor med of primit ive cells  i n  the e x pandedp r od ucts  as an indication of the effect ofexpans io n  o n lon g t erm engraf ti ng ce l ls (ste m  c e l l s).

studies_is to_evaluate improved expansion culture_conditions in_clinical_trials. Preclinical_studies_will also be_performed to evaluate_other culture conditions that_may provide enhanced_platelet_recovery. In addition, analysis will be performed of primitive cells in the expanded products_as_an indication_of_the_effect of expansion on long_term engrafting cells (stem cells).


% between the small subunits of the enzymes. The GAT subunits perform identical functions, and differ only in the specific interactions with the appropriate large subunit. The nucleotide sequence of a GAT subunit that functions in both anthranilate and PABA synthesis in A. calcoaceticus has also been determined, and is very

% between the small subunits of the enzymes. The GAT subunits do identical function, and differ only in the specific interaction with the appropriate big subunit. The nucleotide sequence of a GAT fractional monetary unit that functions in both anthranilate and PABA synthesis in A. calcoaceticus has also been determine, and is very

% behween the small subunits of the enzymes. The GAV subunjts perfurm identical functions, and viffwr onoy in the specific intdractions with thw apkropriate large sngunit. Tmz nucmcotidz wequence of a NAT subunit that functionv kn both anthranilate and PABA synthesys in A. cwlcoaceticus hws akfo bsvn determined, and is very

% between the small subunits of the GAT perform identical and differ only the large subunit. The sequence of a subunit that functions in both anthranilate PABA synthesis in A. calcoaceticus has also been determined, and is very

% between the small subunits of The enzymes. the GAt suBunItS perForm Identical functIOns, aNd differ only in the speciFic inTeRActiONs With tHe approPRiATE laRgE sUbuNiT. thE nuclEotIde sequEnce of a GAT SubUnIt that functiONs In both anthRanIlate and PABA SynThesis In a. caLCoaceTicUs has Also beEN deterMined, and iS vERy

% between the small subuni ts of theenzym es.  Th eGATsubu nits perform i d enti cal functions, and dif fer o nl y  int he  spec ific in t er a c tio ns  w ith  t h eappro pri ate lar ge subunit . T he  nucleotides eq uence of a  GA T subunit th atfuncti on s i n  both  an thran ilatea nd PAB A synthes is in A.c alcoace t i cu s ha s also been deter m in e d, and is very

% between_the small_subunits of the enzymes._The GAT_subunits_perform identical_functions,_and differ only_in the specific_interactions with the appropriate_large subunit. The_nucleotide_sequence of a GAT subunit that functions in both anthranilate and PABA synthesis in_A._calcoaceticus has_also_been_determined, and is very


of in vivo studies (animal models and clinic data). Pathway-selective compounds could have functional selectivity. We also plan to demonstrate assay feasibility for HTS. Dr. Wei Zheng's group in NCATS will conduct the pilot screenings using the LOPAC library. Based on the results of the aim 4,

of in vivo studies (animal models and clinic data). Pathway - selective compound could take functional selectivity. We also plan to prove assay feasibility for HTS. Dr. Wei Zheng's group in NCATS will conduct the pilot screening use the LOPAC library. Based on the consequence of the aim 4,

of in vivo studies (animal oodels and clinnx data). Pathwzy-selectkve compounds could have funrtiobal stjectivity. We also olan to dvmonstratw aswqy feasibility for HTS. Dd. Wei Vheng's group in NCATS wiln conduct the [iuoc screenings using the LOPAC library. Based pn the results os tht aym 4,

of in vivo studies (animal models and Pathway-selective could have selectivity. We also for Dr. Wei Zheng's in NCATS will the pilot screenings using the LOPAC Based on the results of the aim 4,

of in vivo studies (animal modeLs and cliniC data). patHwaY-sElecTive Compounds could HAve fUnctional selectivity. We Also pLaN To deMOnStratE assay fEAsIBIliTy FoR HTs. DR. weI ZhenG's gRoup in NcATS will coNduCt The pilot screENiNgs using thE LOpAC library. BaSed On the rEsUltS Of the Aim 4,

of in vivo studies (anima l models a nd cl ini c d at a).Path way-selectivec ompo unds could have functi onalse l ecti v it y. We  also p l an t o d em on str at e  a ssayfea sibilit y for HTS.  Dr .Wei Zheng'sg ro up in NCAT S w ill conductthe  pilot  s cre e nings  us ing t he LOP A C libr ary. Base do n ther esultso f  t he a im 4,

of_in vivo_studies (animal models and_clinic data)._Pathway-selective_compounds could_have_functional selectivity. We_also plan to_demonstrate assay feasibility for_HTS. Dr. Wei_Zheng's_group in NCATS will conduct the pilot screenings using the LOPAC library. Based on_the_results of_the_aim_4,


cancer cells during mitosis, we will challenge traditional biochemical views of gene regulation by defining the pathological linkages between modifications in nuclear architecture and gene expression that are fundamental to the molecular etiology of tumorigenesis. RELEVANCE (See instructions): Changes in the overall shape and structure ofthe nucleus are pathological hallmarks of cancer cells that

cancer cells during mitosis, we will challenge traditional biochemical views of gene regulation by defining the diseased linkage between modifications in nuclear architecture and gene formula that are cardinal to the molecular etiology of tumorigenesis. RELEVANCE (See instructions ): Changes in the overall form and structure ofthe nucleus are diseased hallmark of cancer cells that

cajcer cells during mitosir, we will challgnte travitionam biocheoical views of gene regulatiln by dtyining the pathologizal linkahes betwwen nidificatioia in nugjear wrchntxcture and gene expressiot that are fungaoeutal to the molecular etiology of tuiorigenrsls. RELEVANCE (Sge inxerucfpoks): Changes in the overall shape znd strlcture ofthe nuclrus are pathological hallmwrks of cancer cells tjat

cancer cells during mitosis, we will challenge views gene regulation defining the pathological architecture gene expression that fundamental to the etiology of tumorigenesis. RELEVANCE (See instructions): in the overall shape and structure ofthe nucleus are pathological hallmarks of cancer that

cancer cells during mitosis, wE will challEnge tRadItiOnAl biOcheMical views of geNE regUlation by defining the paTholoGiCAl liNKaGes beTween moDIfICAtiOnS iN nuClEAr ArchiTecTure and Gene expresSioN tHat are fundamENtAl to the molEcuLar etiology oF tuMorigeNeSis. reLEVAnCE (see inStructIOns): ChaNges in the OvERall shAPe and stRUCtUre oFthe nucleus are patHOlOGical hallmarks Of cancEr CElLS ThaT

cancer cells during mitos is, we wil l cha lle nge  t radi tion al biochemical view s of gene regulation b y def in i ng t h epatho logical li n k age sbe twe en mo dific ati ons innuclear ar chi te cture and ge n eexpression  th at are funda men tal to  t hem olecu lar  etio logy o f  tumor igenesis.  R E LEVANC E  (See i n s tr ucti ons): Changes int he overall shapeand st ru c tu r e  of the  nucleus a re  path o logical ha l l m ark s  of cancer ce lls that

cancer_cells during_mitosis, we will challenge_traditional biochemical_views_of gene_regulation_by defining the_pathological linkages between_modifications in nuclear architecture_and gene expression_that_are fundamental to the molecular etiology of tumorigenesis. RELEVANCE (See instructions): Changes in the_overall_shape and_structure_ofthe_nucleus are pathological hallmarks of_cancer cells that


novel anti-complement proteins. Proteins produced by ticks are attractive vaccine targets because such vaccines have the potential to block tick feeding and pathogen transmission. Under aim 3 we plan to test anti-complement proteins as vaccines that block feeding and/or pathogen transmission. Our industry partner in these studies is L2 diagnostics, which

novel anti - complement proteins. Proteins produced by check mark are attractive vaccine target because such vaccines have the potential to obstruct tick feeding and pathogen infection. Under bearing 3 we plan to screen anti - complement proteins as vaccines that stop feeding and/or pathogen transmission. Our industry partner in these studies is L2 diagnostics, which

nogel anti-complement protelns. Proteins probyced bb ticks are attfactive vaccine targets becanse wuch caccines have the potevtial to hlock tixk fteding and pathogxh transmissioh. Undzr aim 3 we plan jo test anti-womplement prodeknd as vaccines that block feeding anq/or patnohen transmissijn. Olr indhstry partner in these studies is M2 diagnmstics, which

novel anti-complement proteins. Proteins produced by ticks vaccine because such have the potential pathogen Under aim 3 plan to test proteins as vaccines that block feeding pathogen transmission. Our industry partner in these studies is L2 diagnostics, which

novel anti-complement proteiNs. Proteins ProduCed By tIcKs arE attRactive vaccine TArgeTs because such vaccines hAve thE pOTentIAl To bloCk tick fEEdING anD pAtHogEn TRaNsmisSioN. Under aIm 3 we plan to TesT aNti-complemenT PrOteins as vaCciNes that block FeeDing anD/oR paTHogen TraNsmisSion. OuR IndustRy partner In THese stUDies is L2 DIAgNostIcs, which

novel anti-complement pro teins. Pro teins  pr odu ce d by  tic ks are attract i ve v accine targets because  such  v a ccin e shavethe pot e nt i a l t obl ock  t i ck  feed ing  and pa thogen tra nsm is sion. Undera im  3 we plan  to  test anti-c omp lement  p rot e ins a s v accin es tha t  block  feedingan d /or pa t hogen t r a ns miss ion. Our industry pa r tner in thesestudie si sL 2  di agn ostics, wh ic h

novel_anti-complement proteins._Proteins produced by ticks_are attractive_vaccine_targets because_such_vaccines have the_potential to block_tick feeding and pathogen_transmission. Under aim_3_we plan to test anti-complement proteins as vaccines that block feeding and/or pathogen transmission._Our_industry partner_in_these_studies is L2 diagnostics, which


ap experienced by persons who have hearing loss. Over a five-year period 200 subjects, selected on the basis of age and hearing status, will be enrolled in a set of cross-sectional experiments. The experimental methodologies have been developed and tested through a series of extensive pilot studies. The experiments are designed to assess subjects'

ap experienced by persons who have hearing loss. Over a five - class time period 200 subjects, selected on the footing of age and hear status, will be enroll in a bent of cross - sectional experiments. The experimental methodologies have been developed and test through a series of extensive original sketch. The experiments are designed to assess subjects'

ap fxperienced by persons wmo have hearing loss. Ovxr a fibe-year pdriod 200 subjects, selected on vhe vasis of age and hearing stxtus, will be enrooled un a set oh cross-scetionzp ex'eciments. The expgrimental medhodologies haee bzen developed and tested through a sqries og fxtensive piloj stucyes. Fhe experiments are designed to asaess suujects'

ap experienced by persons who have hearing a period 200 selected on the status, be enrolled in set of cross-sectional The experimental methodologies have been developed tested through a series of extensive pilot studies. The experiments are designed to subjects'

ap experienced by persons who Have hearinG loss. oveR a fIvE-yeaR perIod 200 subjects, selECted On the basis of age and hearIng stAtUS, wilL Be EnrolLed in a sET oF CRosS-sEcTioNaL ExPerimEntS. The expErimental mEthOdOlogies have bEEn Developed aNd tEsted through A seRies of ExTenSIve piLot StudiEs. The eXPerimeNts are desIgNEd to asSEss subjECTs'

ap experienced by personswho have h earin g l oss .Over  a f ive-year perio d  200  subjects, selected on  theba s is o f  a ge an d heari n gs t atu s,  w ill  b e  e nroll edin a se t of cross -se ct ional experi m en ts. The ex per imental meth odo logies  h ave beendev elope d andt estedthrough a  s e ries o f  extens i v epilo t studies. The ex p er i ments are desi gned t oa ss e s s s ubj ects'

ap experienced_by persons_who have hearing loss._Over a_five-year_period 200_subjects,_selected on the_basis of age_and hearing status, will_be enrolled in_a_set of cross-sectional experiments. The experimental methodologies have been developed and tested through a_series_of extensive_pilot_studies._The experiments are designed to_assess subjects'


effector molecule for this activator. Urea is Found at concentrations up to 500 mM in the urinary tract, a concentration that is at least 50 fold higher than that observed at other sites in the body. Thus for these uropathogens, urea is a signal molecule, and UreR is acting as a signal receptor

effector molecule for this activator. Urea is Found at concentrations up to 500 mM in the urinary nerve pathway, a assiduity that is at least 50 flock higher than that note at early sites in the body. Thus for these uropathogens, urea is a signal atom, and UreR is act as a signal receptor

efvector molecule for this activator. Urea is Fouid at ckncentragions up to 500 mM in the urinacy teact, q concentration that ir at leasn 50 fold hughec than that obsecbed at other altes nn the body. Thus for these uropathogens, grda is a signal molecule, and UreR is asting ax w signal recepjor

effector molecule for this activator. Urea is concentrations to 500 in the urinary at 50 fold higher that observed at sites in the body. Thus for uropathogens, urea is a signal molecule, and UreR is acting as a signal

effector molecule for this acTivator. UreA is FoUnd At cOnCentRatiOns up to 500 mM in the URinaRy tract, a concentration tHat is At LEast 50 FOlD highEr than tHAt OBSerVeD aT otHeR SiTes in The Body. ThuS for these uRopAtHogens, urea is A SiGnal molecuLe, aNd UreR is actiNg aS a signAl RecEPtor

effector molecule for thi s activato r. Ur eaisFo undat c oncentrationsu p to  500 mM in the urinary  trac t, a co n ce ntrat ion tha t  i s atle as t 5 0f ol d hig her  than t hat observ edat  other sites in  the body.  Th us for these  ur opatho ge ns, ureaisa sig nal mo l ecule,  and UreR  i s  actin g  as a s i g na l re ceptor

effector_molecule for_this activator. Urea is_Found at_concentrations_up to_500_mM in the_urinary tract, a_concentration that is at_least 50 fold_higher_than that observed at other sites in the body. Thus for these uropathogens, urea_is_a signal_molecule,_and_UreR is acting as a_signal receptor


functional and pathological conditions of extremity soft tissue. The imaging capabilities of this novel technology and the use of low, safe levels of non-ionizing radiation with cost efficiency have potentials for significant improvement of public healthcare. [unreadable] [unreadable] [unreadable] Sounds are encoded by the cochlea in the timing of spikes in

functional and pathological conditions of extremity soft tissue. The image capability of this novel technology and the use of humble, safe levels of non - ionizing radiotherapy with price efficiency have potential for significant improvement of public healthcare. [ unreadable ] [ indecipherable ] [ unreadable ] Sounds are encoded by the cochlea in the timing of spikes in

fujctional and pathologicau conditions of extremmty soff tissue. The imaging capabilities of tyis nivel technology and thd use of pow, safe levtls of non-ionizinj radiatljn wjbh covv efficiency haye potentians for signifiwavt improvement of public healthcare. [ugreadabke] [unreadable] [unteadanje] Sklnbs are encoded by the cochlea ih the tpming of spikes im

functional and pathological conditions of extremity soft imaging of this technology and the of radiation with cost have potentials for improvement of public healthcare. [unreadable] [unreadable] Sounds are encoded by the cochlea in the timing of spikes in

functional and pathological Conditions Of extRemIty SoFt tiSsue. the imaging capaBIlitIes of this novel technoloGy and ThE Use oF LoW, safe Levels oF NoN-IOniZiNg RadIaTIoN with CosT efficiEncy have poTenTiAls for signifICaNt improvemEnt Of public healThcAre. [unrEaDabLE] [unreAdaBle] [unReadabLE] SoundS are encodEd BY the coCHlea in tHE TiMing Of spikes in

functional and pathologic al conditi ons o f e xtr em itysoft  tissue. The i m agin g capabilities of this  nove lt echn o lo gy an d the u s eo f  lo w,  s afe  l e ve ls of  no n-ioniz ing radiat ion  w ith cost eff i ci ency havepot entials forsig nifica nt  im p rovem ent  of p ublich ealthc are. [unr ea d able][ unreada b l e]  [un readable] Soundsa re encoded by the  cochl ea in t hetim ing of spi ke s in

functional_and pathological_conditions of extremity soft_tissue. The_imaging_capabilities of_this_novel technology and_the use of_low, safe levels of_non-ionizing radiation with_cost_efficiency have potentials for significant improvement of public healthcare. [unreadable] [unreadable] [unreadable] Sounds are_encoded_by the_cochlea_in_the timing of spikes in


RS using extensive computation to simultaneously maximize spectral resolution and sensitivity to nuclear spin relaxation we have developed a novel multiparametric technique that can simultaneously measure concentration, longitudinal and transverse relaxation times of many brain chemicals, including glutamate, in a single scan session within ten minutes of MRS data acquisition. This multiparametric approach allows us

RS using extensive computation to simultaneously maximize spectral settlement and sensitivity to nuclear tailspin relaxation we have developed a fresh multiparametric technique that can simultaneously quantify concentration, longitudinal and transverse easiness times of many brain chemical, include glutamate, in a single scan seance within ten minutes of MRS data skill. This multiparametric overture allows us

RS ksing extensive computatlon to simultaneously meximize spectrau resolution and sensitivity ti nucoear spin relaxation wd have deneloped a novtl multiparametrir technique thzb can wimultaneously measure cmncentration, lmneicudinal and transverse relaxation tiies of kajy brain chemisals, ynclhding glutamate, in a single scan ssssion xithin ten minuyes of MRS data acquisitioj. Thls multiparametric approach aolowf us

RS using extensive computation to simultaneously maximize and to nuclear relaxation we have that simultaneously measure concentration, and transverse relaxation of many brain chemicals, including glutamate, a single scan session within ten minutes of MRS data acquisition. This multiparametric allows us

RS using extensive computatiOn to simultAneouSly MaxImIze sPectRal resolution aND senSitivity to nuclear spin rElaxaTiON we hAVe DevelOped a noVEl MULtiPaRaMetRiC TeChniqUe tHat can sImultaneouSly MeAsure concentRAtIon, longituDinAl and transveRse RelaxaTiOn tIMes of ManY braiN chemiCAls, incLuding gluTaMAte, in a SIngle scAN SeSsioN within ten minutes OF Mrs data acquisitiOn. This MuLTiPARamEtrIc approach AlLows uS

RS using extensive computa tion to si multa neo usl ymaxi mize  spectral reso l utio n and sensitivity to n uclea rs pinr el axati on we h a ve d eve lo pe d a  n o ve l mul tip arametr ic techniq ueth at can simul t an eously mea sur e concentrat ion , long it udi n al an d t ransv erse r e laxati on timesof many b r ain che m i ca ls,including glutama t e, in a single sc an ses si o nw i thi n t en minutes  o f MRS data ac q ui s i t ion .  This multipa rametric ap p roa ch all ow s u s

RS using_extensive computation_to simultaneously maximize spectral_resolution and_sensitivity_to nuclear_spin_relaxation we have_developed a novel_multiparametric technique that can_simultaneously measure concentration,_longitudinal_and transverse relaxation times of many brain chemicals, including glutamate, in a single scan_session_within ten_minutes_of_MRS data acquisition. This multiparametric_approach allows us


of memory formation 2. Behavior-genetic analyses of associative learning 3. Behavior-genetic analyses of non-associative learning 4. Anatomy and development of learning and memory 5. Analyses of sensorimotor controls One curious aspect of drug addiction is that a majority of people experimenting with illicit drugs do not develop the

of memory formation 2. Behavior - genetic analyses of associative eruditeness 3. demeanor - genic analyses of non - associative eruditeness 4. Anatomy and exploitation of learning and memory 5. Analyses of sensorimotor control One curious aspect of drug addiction is that a majority of people experiment with illicit drugs do not build up the

of memory formation 2. Behavlor-genetic analyses of essociafive leafning 3. Behavior-genetic analydew of bon-associative learnine 4. Anatomj and devwlopnwnt of leaching and memodn 5. Anclbses of sensorikotor contsols One curiogs adpect of drug addiction is that a mwjority ov people experymenuind wifh illicit drugs do not develop ths

of memory formation 2. Behavior-genetic analyses of 3. analyses of learning 4. Anatomy memory Analyses of sensorimotor One curious aspect drug addiction is that a majority people experimenting with illicit drugs do not develop the

of memory formation 2. Behavior-Genetic anaLyses Of aSsoCiAtivE leaRning 3. Behavior-gENetiC analyses of non-associatIve leArNIng 4. ANAtOmy anD develoPMeNT Of lEaRnIng AnD MeMory 5. ANalYses of sEnsorimotoR coNtRols One curioUS aSpect of druG adDiction is thaT a mAjoritY oF peOPle exPerImentIng witH IlliciT drugs do nOt DEvelop THe

of memory formation 2. Be havior-gen eticana lys es  ofasso ciative learni n g 3.  Behavior-genetic anal ysesof non- a ss ociat ive lea r ni n g  4.  A na tom ya nd  deve lop ment of  learningand  m emory 5. Ana l ys es of sens ori motor contro lsOne cu ri ous aspec t o f dru g addi c tion i s that ama j orityo f peopl e ex peri menting with illi c it drugs do not d evelop  t h e

of_memory formation_2. Behavior-genetic analyses of_associative learning_3._Behavior-genetic analyses_of_non-associative learning 4._Anatomy and development_of learning and memory_5. Analyses of_sensorimotor_controls One curious aspect of drug addiction is that a majority of people experimenting_with_illicit drugs_do_not_develop the


(a) the Balloon Analogue Risk Task (BART) measure of impulsivity while eliminating the limitation of a self-report measure requiring self-insight and awareness, and (b) Audio Computer Assisted Self Interviewing (ACASI), which is used in behavioral research to minimize recall and social

(a) the Balloon Analogue Risk Task (BART) measure of impulsivity while eliminating the limitation of a self - report standard command self - insight and awareness, and (b) Audio Computer Assisted Self Interviewing (ACASI), which is used in behavioral research to understate recall and social

(a) hhe Balloon Analogue Risy Task (BART) measure of impulaivity wfile eliminating the limitatmon if a welf-report measure reqjiring sepf-insighr anv awareness, and (u) Audio Gjmpufcr Asvmsted Self Intetviewing (ACAVI), which is usad iu behavioral research to minimize resall anc docial

(a) the Balloon Analogue Risk Task (BART) impulsivity eliminating the of a self-report and Audio Computer Assisted Interviewing (ACASI), which used in behavioral research to minimize and social

(a) the Balloon Analogue Risk TaSk (BART) measUre of ImpUlsIvIty wHile Eliminating the LImitAtion of a self-report measUre reQuIRing SElF-insiGht and aWArENEss, AnD (b) audIo cOmPuter assIsted SeLf IntervieWinG (AcASI), which is uSEd In behaviorAl rEsearch to minImiZe recaLl And SOcial

(a) the Balloon AnalogueRisk Task(BART ) m eas ur e of  imp ulsivity while elim inating the limitation  of a  s e lf-r e po rt me asure r e qu i r ing  s el f-i ns i gh t and  aw areness , and (b)Aud io  Computer As s is ted Self I nte rviewing (AC ASI ), whi ch  is usedinbehav ioralr esearc h to mini mi z e reca l l and s o c ia l

(a)_the Balloon_Analogue Risk Task (BART)_measure of_impulsivity_while eliminating_the_limitation of a_self-report measure requiring_self-insight and awareness, and_(b) Audio Computer_Assisted_Self Interviewing (ACASI), which is used in behavioral research to minimize recall and social


analysis and interpretation of cancer clinical trials, cancer population studies and cancer genomic studies. All predoctoral students supported by this training grant are required to take a concentration in cancer-related courses. During their first and second summer periods in the program, students are required to participate in research activities of the DFCI or

analysis and interpretation of cancer clinical trials, cancer population studies and cancer genomic report. All predoctoral student supported by this training grant are ask to take a concentration in cancer - relate class. During their inaugural and second summer periods in the program, scholar are required to participate in inquiry activities of the DFCI or

anwlysis and interpretatiok of cancer clinnxal trmals, cahcer popjlation studies and cancer gxnomuc stydies. All predoctoral rtudents dupportee by rhis trainmhg granb are vequixev to take a congentration hn cancer-relatad clurses. During their first and seconq summet oeriods in the probwam, anubents are required to participafe in rtsearch activities of the DFCI or

analysis and interpretation of cancer clinical trials, studies cancer genomic All predoctoral students are to take a in cancer-related courses. their first and second summer periods the program, students are required to participate in research activities of the DFCI

analysis and interpretation Of cancer clInicaL trIalS, cAnceR popUlation studies ANd caNcer genomic studies. All pRedocToRAl stUDeNts suPported BY tHIS trAiNiNg gRaNT aRe reqUirEd to takE a concentrAtiOn In cancer-relaTEd Courses. DurIng Their first anD seCond suMmEr pERiods In tHe proGram, stUDents aRe requireD tO ParticIPate in rESEaRch aCtivities of the DFCi Or

analysis and interpretati on of canc er cl ini cal  t rial s, c ancer populati o n st udies and cancer genom ic st ud i es.A ll  pred octoral st u d ent ssu ppo rt e dby th istrainin g grant ar e r eq uired to tak e  a  concentra tio n in cancer- rel ated c ou rse s . Dur ing  thei r firs t  and s econd sum me r  perio d s in th e pr ogra m, students are r e qu i red to partici pate i nr es e a rch  ac tivities o fthe D F CI or

analysis_and interpretation_of cancer clinical trials,_cancer population_studies_and cancer_genomic_studies. All predoctoral_students supported by_this training grant are_required to take_a_concentration in cancer-related courses. During their first and second summer periods in the program,_students_are required_to_participate_in research activities of the_DFCI or


2 on Chr 1 at ~ 110 and 175 Mbp) and PPI (Chr 11 at ~ 70 Mbp and Chr 16 at ~ 45 Mbp). 2) To integrate the QTL data with interval relevant gene expression and sequence data. 3) To determine in HS4 animals the gene coexpression networks associated with OF

2 on Chr 1 at ~ 110 and 175 Mbp) and PPI (Chr 11 at ~ 70 Mbp and Chr 16 at ~ 45 Mbp). 2) To integrate the QTL data with interval relevant gene expression and succession datum. 3) To specify in HS4 animals the gene coexpression networks consort with OF

2 on Chr 1 at ~ 110 and 175 Mbp) and KPI (Chr 11 at ~ 70 Mbp and Chc 16 at ~ 45 Mbp). 2) To integrate the QTL data with ibtervql relevant gene exprersion and sequencw daua. 3) To determine mh HS4 anlials bhe gznx coexpression ketworks asvociated with MF

2 on Chr 1 at ~ 110 Mbp) PPI (Chr at ~ 70 ~ Mbp). 2) To the QTL data interval relevant gene expression and sequence 3) To determine in HS4 animals the gene coexpression networks associated with OF

2 on Chr 1 at ~ 110 and 175 Mbp) and PPI (Chr 11 at ~ 70 MBp and Chr 16 at ~ 45 mbp). 2) To IntEgrAtE the qTL dAta with intervaL ReleVant gene expression and sEquenCe DAta. 3) TO DeTermiNe in HS4 aNImALS thE gEnE coExPReSsion NetWorks asSociated wiTh Of

2 on Chr 1 at ~ 110 and 17 5 Mbp) and  PPI(Ch r 1 1at ~  70Mbp and Chr 16 at ~  45 Mbp). 2) To integr ate t he QTLd at a wit h inter v al r ele va nt  ge ne ex press ion  and se quence dat a.3)  To determin e  i n HS4 anim als  the gene co exp ressio nnet w orksass ociat ed wit h  OF

2 on_Chr 1_at ~ 110 and_175 Mbp)_and_PPI (Chr_11_at ~ 70_Mbp and Chr_16 at ~ 45_Mbp). 2) To_integrate_the QTL data with interval relevant gene expression and sequence data. 3) To determine_in_HS4 animals_the_gene_coexpression networks associated with OF


examine whether learned control mechanisms develop without explicit laboratory training, whether consumption of high-fat diets interferes with these mechanisms, and whether this interference has potential to produce significant weight gain. These studies promise to yield important new information about the role of dietary factors in both obesity and cognitive decline. PUBLIC HEALTH RELEVANCE

examine whether learned control mechanisms originate without denotative laboratory training, whether pulmonary tuberculosis of high - fatness diets interferes with these mechanism, and whether this interference has electric potential to grow significant weight unit gain. These studies predict to yield important new information about the role of dietary factors in both obesity and cognitive decay. PUBLIC HEALTH RELEVANCE

exwmine whether learned coktrol mechanisms develo' withoht explizit laboratory training, whetier xonsunption of high-fat dietr interfeges with rhest mechanisms, and xgether bkis ihberfexeice has potentisl to prodgce significand denght gain. These studies promise to yyeld imlogtant new infotmatipg abklt the role of dietary factors jn both obesity and vognitive decline. PUBLIC HFALTJ RELEVANCE

examine whether learned control mechanisms develop without training, consumption of diets interferes with interference potential to produce weight gain. These promise to yield important new information the role of dietary factors in both obesity and cognitive decline. PUBLIC HEALTH

examine whether learned contRol mechaniSms deVelOp wItHout ExplIcit laboratory TRainIng, whether consumption oF high-FaT DietS InTerfeRes with THeSE MecHaNiSms, AnD WhEther ThiS interfErence has pOteNtIal to produce SIgNificant weIghT gain. These stUdiEs promIsE to YIeld iMpoRtant New infORmatioN about the RoLE of dieTAry factORS iN botH obesity and cognitIVe DEcline. PUBLIC HEaLTH RElEvaNce

examine whether learned c ontrol mec hanis msdev el op w itho ut explicit la b orat ory training, whetherconsu mp t iono fhigh- fat die t si n ter fe re s w it h  t hesemec hanisms , and whet her  t his interfer e nc e has pote nti al to produc e s ignifi ca ntw eight  ga in. T hese s t udiespromise t oy ield i m portant n ew  inf ormation about th e  r o le of dietaryfactor si nb o thobe sity and c og nitiv e  declin e .P U B LIC HEALTH RELEVA NCE

examine_whether learned_control mechanisms develop without_explicit laboratory_training,_whether consumption_of_high-fat diets interferes_with these mechanisms,_and whether this interference_has potential to_produce_significant weight gain. These studies promise to yield important new information about the role_of_dietary factors_in_both_obesity and cognitive decline. PUBLIC_HEALTH RELEVANCE


F-1 can augment bone regeneration in aged mice, a model of suboptimal healing. If our hypotheses are correct, our studies will provide convincing evidence that SDF-1 works in a paracrine fashion in bone, a finding that would significantly advance our understanding of skeletal homeostasis and serve as a basis for developing therapeutic

F-1 can augment bone regeneration in aged mice, a model of suboptimal healing. If our hypotheses are right, our cogitation will provide convincing evidence that SDF-1 work in a paracrine manner in bone, a finding that would significantly advance our agreement of skeletal homeostasis and serve as a footing for develop curative

F-1 cwn augment bone regenerauion in aged mice, a moden of shboptimau healing. If our hypotheses ere xorrext, our studies will pruvide connincing ecideice that SDF-1 works in a icracrjke faviion in bone, a ninding thad would signifhcxncly advance our understanding of skejetal hpmfostasis and sgrve sf a gasis for developing therapeutic

F-1 can augment bone regeneration in aged model suboptimal healing. our hypotheses are convincing that SDF-1 works a paracrine fashion bone, a finding that would significantly our understanding of skeletal homeostasis and serve as a basis for developing therapeutic

F-1 can augment bone regeneratiOn in aged miCe, a moDel Of sUbOptiMal hEaling. If our hypOThesEs are correct, our studies Will pRoVIde cONvIncinG evidenCE tHAT SDf-1 wOrKs iN a PArAcrinE faShion in Bone, a findiNg tHaT would signifICaNtly advancE ouR understandiNg oF skeleTaL hoMEostaSis And seRve as a BAsis foR developiNg THerapeUTic

F-1 can augment bone regen eration in  aged  mi ce,  a  mod el o f suboptimal h e alin g. If our hypotheses a re co rr e ct,o ur  stud ies wil l  p r o vid eco nvi nc i ng  evid enc e thatSDF-1 work s i na paracrinef as hion in bo ne,  a finding t hat  would  s ign i fican tly  adva nce ou r  under standingof skelet a l homeo s t as is a nd serve as a bas i sf or developingtherap eu t ic

F-1 can_augment bone_regeneration in aged mice,_a model_of_suboptimal healing._If_our hypotheses are_correct, our studies_will provide convincing evidence_that SDF-1 works_in_a paracrine fashion in bone, a finding that would significantly advance our understanding of_skeletal_homeostasis and_serve_as_a basis for developing therapeutic


the presence of cyclic nucleotides in canine gastric secretions. BIBLIOGRAPHIC REFERENCE: Schwartzel, E.H., Jr., Bachman, S., and Levine, R.A.: Cyclic nucleotide activity in gastrointestinal tissues and fluids. Fed. Proc. 35:583,

the presence of cyclic nucleotides in canine gastric secretions. BIBLIOGRAPHIC REFERENCE: Schwartzel, E.H., Jr., Bachman, S., and Levine, R.A.: Cyclic nucleotide natural process in gastrointestinal tissue and fluids. Fed. Proc. 35:583,

thf presence of cyclic nucueotides in cannbe gasvric sedretions. BIBLIOGRAPHIC REFERENCE: Schxartzel, E.Y., Jr., Bachman, S., and Levkne, R.A.: Cyblic nuclwotive activity in geatrointcftinzp tivwues and fluidx. Fed. Proc. 35:583,

the presence of cyclic nucleotides in canine BIBLIOGRAPHIC Schwartzel, E.H., Bachman, S., and in tissues and fluids. Proc. 35:583,

the presence of cyclic nucleoTides in canIne gaStrIc sEcRetiOns. BiBLIOGRAPHIC REfeRENcE: Schwartzel, E.H., Jr., BachmaN, S., and leVIne, R.a.: cyClic nUcleotiDE aCTIviTy In GasTrOInTestiNal Tissues And fluids. FEd. PRoC. 35:583,

the presence of cyclic nu cleotidesin ca nin e g as tric  sec retions. BIBLI O GRAP HIC REFERENCE: Schwart zel,E. H ., J r .,  Bach man, S. ,  a n d  Le vi ne , R .A . :Cycli c n ucleoti de activit y i ngastrointest i na l tissuesand  fluids. Fed . P roc. 3 5: 583 ,

the_presence of_cyclic nucleotides in canine_gastric secretions._BIBLIOGRAPHIC_REFERENCE: Schwartzel,_E.H.,_Jr., Bachman, S.,_and Levine, R.A.:_Cyclic nucleotide activity in_gastrointestinal tissues and_fluids._Fed. Proc. 35:583,


) of selected cell composition. The pattern of i-cell behavior will be analyzed quantitatively as methods have been developed for quantitatively measuring all cell populations in hydra, as well as examining quantitatively the behavior of a cohort of isotopically-labeled cells with autoradiography. The objects of the proposal are to: (1) study

) of selected cell composition. The pattern of one - cellular telephone demeanor will be analyzed quantitatively as methods have been developed for quantitatively quantify all cell populations in hydra, equally well as examining quantitatively the behavior of a cohort of isotopically - label cells with autoradiography. The objects of the marriage proposal are to: (1) study

) of selected cell compositiun. The pattern of i-celn behabior wilu be analyzed quantitatively aw metyods have been developdd for quwntitaticely neasuring eml cell populzbions mn hydra, as welk as examiting quantitathvdlv the behavior of a cohort of isotopycally-lsbfled cells witr auuorwdiofgakhy. The objects of the proposal ade to: (1) vtudy

) of selected cell composition. The pattern behavior be analyzed as methods have all populations in hydra, well as examining the behavior of a cohort of cells with autoradiography. The objects of the proposal are to: (1) study

) of selected cell composition. the pattern Of i-ceLl bEhaViOr wiLl be Analyzed quantiTAtivEly as methods have been deVelopEd FOr quANtItatiVely meaSUrING alL cElL poPuLAtIons iN hyDra, as weLl as examinIng QuAntitatively THe Behavior of A coHort of isotopIcaLly-labElEd cELls wiTh aUtoraDiograPHy. The oBjects of tHe PRoposaL Are to: (1) stUDY

) of selected cell composi tion. Thepatte rnofi- cell  beh avior will bea naly zed quantitatively asmetho ds have be en de veloped fo r qua nt it ati ve l ymeasu rin g all c ell popula tio ns  in hydra, a s  w ell as exa min ing quantita tiv ely th ebeh a viorofa coh ort of isotop ically-la be l ed cel l s witha u to radi ography. The obje c ts of the proposa l areto :  ( 1 )  st udy

) of_selected cell_composition. The pattern of_i-cell behavior_will_be analyzed_quantitatively_as methods have_been developed for_quantitatively measuring all cell_populations in hydra,_as_well as examining quantitatively the behavior of a cohort of isotopically-labeled cells with autoradiography._The_objects of_the_proposal_are to: (1) study


iosus, and eight had relatively small septal defect with no other major anomalies. Of the four rats with truncus, two had a vestigial vasculature on the truncus root and three had a rudimentary infundibulum. In 44 rats (68%) the middle latitudinal muscle bundle of the

iosus, and eight had relatively small septal defect with no other major anomaly. Of the four rotter with truncus, two had a vestigial vasculature on the truncus root and three had a vestigial infundibulum. In 44 rats (68 %) the middle latitudinal brawn pile of the

iosks, and eight had relativtly small septal bwfect xith no other mxjor anomalies. Of the four rets qith uguncus, two had a vestkgial vasbulature in tie truncus root ehd threc had w rubinentary infundlbulum. In 44 sats (68%) the middne lctitudinal muscle bundle of the

iosus, and eight had relatively small septal no major anomalies. the four rats vestigial on the truncus and three had rudimentary infundibulum. In 44 rats (68%) middle latitudinal muscle bundle of the

iosus, and eight had relativelY small septAl defEct WitH nO othEr maJor anomalies. Of THe foUr rats with truncus, two haD a vesTiGIal vAScUlatuRe on the TRuNCUs rOoT aNd tHrEE hAd a ruDimEntary iNfundibuluM. In 44 RaTs (68%) the middle lATiTudinal musCle Bundle of the

iosus, and eight had relat ively smal l sep tal  de fe ct w ithno other major anom alies. Of the four rat s wit ht runc u s,  twohad a v e st i g ial  v as cul at u re  on t hetruncus  root andthr ee  had a rudim e nt ary infund ibu lum. In 44 r ats  (68%)  t hem iddle  la titud inal m u scle b undle ofth e

iosus, and_eight had_relatively small septal defect_with no_other_major anomalies._Of_the four rats_with truncus, two_had a vestigial vasculature_on the truncus_root_and three had a rudimentary infundibulum. In 44 rats (68%) the middle latitudinal muscle_bundle_of the


large protein molecules, DNA and other macromolecules. 5. Preparation of platinum replicas of cytoskeletons, partially lysed cells and freeze-fractured/freeze- dried tissues and cells. 6. Chemical fixation, critical point drying, sputter-coating and scanning EM digital imaging of small organisms, organs

large protein molecules, DNA and other macromolecule. 5. readiness of platinum replicas of cytoskeletons, partially lysed cells and freeze - fracture / freeze- dry tissues and cells. 6. Chemical obsession, critical compass point drying, spatter - application and scanning EM digital imaging of little organisms, organs

lagge protein molecules, DNX and other mactonolecunes. 5. Pdeparatiun of platinum replicas of cbtosjeletins, partially lysed ceuls and fgeeze-fracrurev/freeze- dried tissues and cella. 6. Chzmmcal fixation, ctitical poind drying, sputtar-zocting and scanning EM digital imagind of smslp organisms, ordans

large protein molecules, DNA and other macromolecules. of replicas of partially lysed cells cells. Chemical fixation, critical drying, sputter-coating and EM digital imaging of small organisms,

large protein molecules, DNA aNd other macRomolEcuLes. 5. prEparAtioN of platinum repLIcas Of cytoskeletons, partialLy lysEd CElls ANd FreezE-fractuREd/FREezE- dRiEd tIsSUeS and cEllS. 6. ChemicAl fixation, CriTiCal point dryiNG, sPutter-coatIng And scanning Em diGital iMaGinG Of smaLl oRganiSms, orgANs

large protein molecules,DNA and ot her m acr omo le cule s. 5 . Preparationo f pl atinum replicas of cyt oskel et o ns,p ar tiall y lysed ce l l s a nd  f ree ze - fr actur ed/ freeze-  dried tis sue sand cells. 6 .  C hemical fi xat ion, critica l p oint d ry ing ,  sput ter -coat ing an d  scann ing EM di gi t al ima g ing ofs m al l or ganisms, organs

large_protein molecules,_DNA and other macromolecules._5. Preparation_of_platinum replicas_of_cytoskeletons, partially lysed_cells and freeze-fractured/freeze-_dried tissues and cells._6. Chemical fixation,_critical_point drying, sputter-coating and scanning EM digital imaging of small organisms, organs


if metabolites in the urine of mice receiving whole body radiation can predict for radiation-induced cancer induction prior to the observation of tumor mass. Vascular complications in diabetes mellitus are the result of metabolic and hormonal abnormalities caused by insulin deficiency. The identification of specific factors and the mechanisms by which they can cause vascular cell dysfunction and subsequent

if metabolites in the urine of mice receiving whole soundbox radiation sickness can predict for radiation - induced cancer generalization prior to the observation of tumor mass. Vascular complications in diabetes mellitus are the consequence of metabolic and hormonal abnormalities caused by insulin lack. The identification of specific factors and the mechanism by which they can cause vascular cell dysfunction and subsequent

if metabolites in the urint of mice receiviut wholx body dadiatiov can predict for radiation-iiducwd cabcer induction prior tu the obsvrvation if tnmor mass. Vasculed complleatiohd in viabetes mellitos are the rasult of metabmlkc and hormonal abnormalities caused fy insukij deficiency. Tre icqntirpcction of specific factors and tge mechenisms by which they can cause vascular cfll fysfunction and suhsequent

if metabolites in the urine of mice body can predict radiation-induced cancer induction tumor Vascular complications in mellitus are the of metabolic and hormonal abnormalities caused insulin deficiency. The identification of specific factors and the mechanisms by which they cause vascular cell dysfunction and subsequent

if metabolites in the urine of Mice receivIng whOle BodY rAdiaTion Can predict for rADiatIon-induced cancer inductIon prIoR To thE ObServaTion of tUMoR MAss. vaScUlaR cOMpLicatIonS in diabEtes mellitUs aRe The result of mETaBolic and hoRmoNal abnormaliTieS causeD bY inSUlin dEfiCiencY. The idENtificAtion of spEcIFic facTOrs and tHE MeChanIsms by which they caN CaUSe vascular cell DysfunCtIOn AND suBseQuent

if metabolites in the uri ne of mice  rece ivi ngwh olebody  radiation can pred ict for radiation-indu ced c an c er i n du ction  priort ot h e o bs er vat io n  o f tum ormass. V ascular co mpl ic ations in di a be tes mellit usare the resu ltof met ab oli c  andhor monal  abnor m alitie s causedby insuli n  defici e n cy . Th e identificationo fs pecific factor s andth e  m e c han ism s by which  t hey c a n cause va s c u lar cell dysfunct ion and sub s equ ent

if_metabolites in_the urine of mice_receiving whole_body_radiation can_predict_for radiation-induced cancer_induction prior to_the observation of tumor_mass. Vascular complications_in_diabetes mellitus are the result of metabolic and hormonal abnormalities caused by insulin deficiency._The_identification of_specific_factors_and the mechanisms by which_they can cause vascular cell_dysfunction and_subsequent


obstruction of blood flow. In the myocardium this event has been noted for years but only recently has the technique been developed that allows the time related transmural blood flow pattern to this insult to be documented. Two groups of dogs were studied using radioactive microspheres to determine the tramsmural myocardial blood flow during reactive hype

obstruction of blood flow. In the myocardium this event has been note for days but only recently has the technique been evolve that allows the time relate transmural lineage flow form to this insult to be documented. Two group of dogs were studied use radioactive microspheres to determine the tramsmural myocardial rake flow during reactive hype

obdtruction of blood flow. Ln the myocardium this xvent hzs been voted for years but only recxntlt has the technique been dexeloped tjat alloqs tie time related vdansmural blokf flmx pattern to thls insult tm be documenteg. Gwl groups of dogs were studied using radioavtlve microsphergs to qetedmine the tramsmural myocardial blkod flox during reactife hype

obstruction of blood flow. In the myocardium has noted for but only recently that the time related blood flow pattern this insult to be documented. Two of dogs were studied using radioactive microspheres to determine the tramsmural myocardial blood during reactive hype

obstruction of blood flow. In tHe myocardiUm thiS evEnt HaS beeN notEd for years but oNLy reCently has the technique bEen deVeLOped THaT alloWs the tiME rELAteD tRaNsmUrAL bLood fLow Pattern To this insuLt tO bE documented. TWO gRoups of dogS weRe studied usiNg rAdioacTiVe mICrospHerEs to dEtermiNE the trAmsmural mYoCArdial BLood floW DUrIng rEactive hype

obstruction of blood flow . In the m yocar diu m t hi s ev enthas been noted foryears but only recentl y has  t h e te c hn iquebeen de v el o p edth at  al lo w sthe t ime  relate d transmur albl ood flow pat t er n to thisins ult to be do cum ented.  T wog roups  of  dogs  weres tudied  using ra di o active microsp h e re s to  determine the tr a ms m ural myocardia l bloo df lo w dur ing  reactivehy pe

obstruction_of blood_flow. In the myocardium_this event_has_been noted_for_years but only_recently has the_technique been developed that_allows the time_related_transmural blood flow pattern to this insult to be documented. Two groups of dogs_were_studied using_radioactive_microspheres_to determine the tramsmural myocardial_blood flow during reactive hype


and conduct a group-randomized clinical trial among 4-H clubs in four states (NJ, OH, MD, and PA). Clubs will be randomly assigned to use the curriculum or continue current practices with the option for delivery at the end of the study. 4-H members (ages 13-15)

and conduct a group - randomized clinical trial among 4 - H club in four state (NJ, OH, MD, and PA). Clubs will be randomly assigned to use the course of study or continue current practices with the option for manner of speaking at the end of the study. 4 - hydrogen members (ages 13 - 15 )

anf conduct a group-randomieed clinical trial among 4-H clugs in fojr states (NJ, OH, MD, and PA). Clnbs qill ve randomly assigned tu use the curricuoum ie continue current practjges wnti the option fot delivery ad the end of tve scudy. 4-H members (ages 13-15)

and conduct a group-randomized clinical trial among in states (NJ, MD, and PA). to the curriculum or current practices with option for delivery at the end the study. 4-H members (ages 13-15)

and conduct a group-randomizeD clinical tRial aMonG 4-H cLuBs in Four States (NJ, OH, MD, anD pA). ClUbs will be randomly assigNed to UsE The cURrIculuM or contINuE CUrrEnT pRacTiCEs With tHe oPtion foR delivery aT thE eNd of the study. 4-h MeMbers (ages 13-15)

and conduct a group-rando mized clin icaltri alam ong4-Hclubs in fours tate s (NJ, OH, MD, and PA) . Clu bs will be  rand omly as s ig n e d t ous e t he cu rricu lum  or con tinue curr ent  p ractices wit h  t he optionfor  delivery at  th e endof  th e  stud y.4-H m embers (ages13-15)

and_conduct a_group-randomized clinical trial among_4-H clubs_in_four states_(NJ,_OH, MD, and_PA). Clubs will_be randomly assigned to_use the curriculum_or_continue current practices with the option for delivery at the end of the study._4-H_members (ages_13-15)