human and mouse tissues, showing tissue-specific expression of alternatively spliced and read-through transcripts. We conducted several yeast two-hybrid screens to identify protein-binding partners for the putative histone methyltransferases. These screens yielded several intriguing candidates that are being characterized further by biochemical and genetic methods. We have constructed a conditional

human and mouse tissues, showing tissue - specific expression of alternatively splice and understand - through transcripts. We conducted several yeast two - loanblend screens to identify protein - binding spouse for the putative histone methyltransferases. These screens yielded respective challenging candidates that are being characterized far by biochemical and genetic methods. We have constructed a conditional

hulan and mouse tissues, shuwing tissue-speeufic eepressikn of algernatively spliced and read-vhroygh teanscripts. We conductea several yeast tqo-hyurid screens to msentify protejk-bindnnj partners for jhe putative histone methyntfausferases. These screens yielded sevewal inttihuing candidatgs thse ars being characterized further by bjochemibal and genetic mrthods. We have constructed a clnditional

human and mouse tissues, showing tissue-specific expression spliced read-through transcripts. conducted several yeast partners the putative histone These screens yielded intriguing candidates that are being characterized by biochemical and genetic methods. We have constructed a conditional

human and mouse tissues, showiNg tissue-spEcifiC exPreSsIon oF altErnatively spliCEd anD read-through transcriptS. We coNdUCted SEvEral yEast two-HYbRID scReEnS to IdENtIfy prOteIn-bindiNg partners For ThE putative hisTOnE methyltraNsfErases. These sCreEns yieLdEd sEVeral IntRiguiNg candIDates tHat are beiNg CHaractERized fuRTHeR by bIochemical and geneTIc MEthods. We have coNstrucTeD A cONDitIonAl

human and mouse tissues,showing ti ssue- spe cif ic  exp ress ion of alterna t ivel y spliced and read-thr oughtr a nscr i pt s. We  conduc t ed s eve ra lyea st tw o-hyb rid  screen s to ident ify  p rotein-bindi n gpartners f orthe putative  hi stoneme thy l trans fer ases.  These screen s yielded  s e verali ntrigui n g  c andi dates that are be i ng characterizedfurthe rb yb i och emi cal and ge ne tic m e thods.W eh a v e c o nstructed a c onditional

human_and mouse_tissues, showing tissue-specific expression_of alternatively_spliced_and read-through_transcripts._We conducted several_yeast two-hybrid screens_to identify protein-binding partners_for the putative_histone_methyltransferases. These screens yielded several intriguing candidates that are being characterized further by biochemical_and_genetic methods._We_have_constructed a conditional


forces, including principal and shear stresses, in experimental models of hypertension and flow overload. The specific aims include studies on alterations in the macro-structure including the length, diameter, and branching pattern of all generations of the coronary tree in response to increased mechanical stresses; alterations in the vessel walls and their micro-structure, including

forces, including principal and shear stresses, in experimental model of high blood pressure and flow overload. The specific aims admit studies on alteration in the macro - structure include the length, diameter, and branching form of all generations of the coronary tree in response to increase mechanical stresses; alterations in the vessel wall and their micro - social organization, including

fogces, including principal and shear stresses, in experjmental oodels of hypertension and fpoq oveeload. The specific aimr include studies on elterations in tis macro-structhve inelnding the lengtm, diameter, dnd branching [agtzrn of all generations of the coronawy tree ij response to yncrtasqd msbhcnical stresses; alterations in fhe vesvel walls and their micro-structure, inclkdinh

forces, including principal and shear stresses, in of and flow The specific aims the including the length, and branching pattern all generations of the coronary tree response to increased mechanical stresses; alterations in the vessel walls and their micro-structure,

forces, including principal aNd shear strEsses, In eXpeRiMentAl moDels of hypertenSIon aNd flow overload. The speciFic aiMs INcluDE sTudieS on alteRAtIONs iN tHe MacRo-STrUcturE inCluding The length, dIamEtEr, and branchiNG pAttern of alL geNerations of tHe cOronarY tRee IN respOnsE to inCreaseD MechanIcal stresSeS; AlteraTIons in tHE VeSsel Walls and their micrO-StRUcture, includinG

forces, including princip al and she ar st res ses ,in e xper imental models of h ypertension and flow o verlo ad .  The sp ecifi c aimsi nc l u dest ud ies  o n  a ltera tio ns in t he macro-s tru ct ure includin g  t he length,  di ameter, andbra nching  p att e rn of  al l gen eratio n s of t he corona ry tree i n  respon s e  t o in creased mechanica l  s t resses; altera tionsin th e ves sel  walls and  t heirm icro-st r uc t u r e,i ncluding

forces,_including principal_and shear stresses, in_experimental models_of_hypertension and_flow_overload. The specific_aims include studies_on alterations in the_macro-structure including the_length,_diameter, and branching pattern of all generations of the coronary tree in response to_increased_mechanical stresses;_alterations_in_the vessel walls and their_micro-structure, including


routine use in the assay. Malignant effusion fluid and patients' sera will be biochemically fractionated and selected fractions will be analyzed for reactivity in the LAI assay. Xenogeneic antisera will be prepared against the purified antigen preparations and they will be immunochemically and biochemically compared by serological

routine use in the assay. Malignant effusion fluid and patients' sera will be biochemically fractionate and choose fractions will be analyzed for responsiveness in the LAI assay. Xenogeneic antisera will be train against the purified antigen preparations and they will be immunochemically and biochemically compared by serologic

roktine use in the assay. Mxlignant effusion fluiv and pztients' rera will be biochemically fcactuonattb and selected fractkons will be analtzed dor reactitjty in bke LAJ asscy. Xenogeneic anjisera will te prepared agdivsc the purified antigen preparations wnd theu aill be immunoshempcwlly and biochemically compared by serklogican

routine use in the assay. Malignant effusion patients' will be fractionated and selected reactivity the LAI assay. antisera will be against the purified antigen preparations and will be immunochemically and biochemically compared by serological

routine use in the assay. MaligNant effusiOn fluId aNd pAtIentS' serA will be biochemICallY fractionated and selectEd fraCtIOns wILl Be anaLyzed foR ReACTivItY iN thE Lai aSsay. XEnoGeneic aNtisera wilL be PrEpared againsT ThE purified aNtiGen preparatiOns And theY wIll BE immuNocHemicAlly anD BiocheMically coMpARed by sERologicAL

routine use in the assay.  Malignant  effu sio n f lu id a nd p atients' seraw illbe biochemically fract ionat ed ands el ected  fracti o ns w ill  b eana ly z ed  forrea ctivity  in the LA I a ss ay. Xenogene i cantisera w ill  be prepared  ag ainstth e p u rifie d a ntige n prep a ration s and the yw ill be immunoc h e mi call y and biochemical l yc ompared by ser ologic al

routine_use in_the assay. Malignant effusion_fluid and_patients'_sera will_be_biochemically fractionated and_selected fractions will_be analyzed for reactivity_in the LAI_assay._Xenogeneic antisera will be prepared against the purified antigen preparations and they will be_immunochemically_and biochemically_compared_by_serological


with the Indian Health Service. - Diabetes mellitus, prevention, clinical trial - Human Subjects Treatment of Central Vein Thrombosis remains unsatisfactory. However, there is strong evidence from a recent study that quick, aggressive therapy with Urokinase for occlusions of recent onset, can produce marked benefits. Prognosis of these

with the Indian Health Service. - Diabetes mellitus, prevention, clinical trial - Human Subjects Treatment of Central Vein Thrombosis remains unsatisfactory. However, there is strong evidence from a late discipline that quick, aggressive therapy with Urokinase for occlusions of late onset, can grow marked benefits. Prognosis of these

wihh the Indian Health Seryice. - Diabetes mgloitus, 'reventjon, clinkcal trial - Human Subjects Tceatnent if Central Vein Thrombusis remapns unsatusfartory. However, thxde is sbxong syidenee from a recent study thad quick, aggresvixe therapy with Urokinase for occlusijns of tefent onset, can proctce jarked benefits. Prognosis of these

with the Indian Health Service. - Diabetes clinical - Human Treatment of Central there strong evidence from recent study that aggressive therapy with Urokinase for occlusions recent onset, can produce marked benefits. Prognosis of these

with the Indian Health ServicE. - Diabetes mEllitUs, pRevEnTion, ClinIcal trial - Human sUbjeCts Treatment of Central VEin ThRoMBosiS ReMains UnsatisFAcTORy. HOwEvEr, tHeRE iS stroNg eVidence From a recenT stUdY that quick, agGReSsive theraPy wIth Urokinase For OcclusIoNs oF RecenT onSet, caN produCE markeD benefits. prOGnosis OF these

with the Indian Health Se rvice. - D iabet esmel li tus,  pre vention, clini c al t rial - Human SubjectsTreat me n t of Ce ntral  Vein T h ro m b osi sre mai ns un satis fac tory. H owever, th ere  i s strong evi d en ce from arec ent study th atquick,  a ggr e ssive  th erapy  withU rokina se for oc cl u sionso f recen t on set,  can produce mark e db enefits. Progn osis o ft he s e

with_the Indian_Health Service. - Diabetes_mellitus, prevention,_clinical_trial -_Human_Subjects Treatment of_Central Vein Thrombosis_remains unsatisfactory. However, there_is strong evidence_from_a recent study that quick, aggressive therapy with Urokinase for occlusions of recent onset,_can_produce marked_benefits._Prognosis_of these


substances across cells injured by a variety of harmful transport of macromolecular substances across cells injured by a variety of harmful agents. This information would be of fundamental importance in understanding the role of macromolecules and their transport process during physiologic and many pathologic conditions. BIBLIOGRAPHIC REFERENCES: Opar

substances across cells injured by a variety of harmful ecstasy of macromolecular meaning across cells injured by a variety of harmful agent. This information would be of fundamental importance in understand the character of macromolecules and their transport process during physiologic and many pathologic condition. BIBLIOGRAPHIC REFERENCES: Opar

suhstances across cells inmured by a variejy of hacmful tdansport of macromolecular substanced qcrosw cells injured by a vxriety of harmful ageits. This informavjon would be kn funbanental importakce in undesstanding the soue of macromolecules and their transpjrt proveds during physyolobyc ahd many pathologic conditions. BIBLJOGRAPHPC REFERENCES: Opat

substances across cells injured by a variety transport macromolecular substances cells injured by This would be of importance in understanding role of macromolecules and their transport during physiologic and many pathologic conditions. BIBLIOGRAPHIC REFERENCES: Opar

substances across cells injuRed by a variEty of HarMfuL tRansPort Of macromoleculAR subStances across cells injuRed by A vARietY Of HarmfUl agentS. thIS InfOrMaTioN wOUlD be of FunDamentaL importancE in UnDerstanding tHE rOle of macroMolEcules and theIr tRanspoRt ProCEss duRinG physIologiC And manY pathologIc COnditiONs. BIBLIogrApHIC rEFERENCES: Opar

substances across cells i njured bya var iet y o fharm fultransport of m a crom olecular substances ac rossce l ls i n ju red b y a var i et y ofha rm ful  a g en ts. T his  inform ation woul d b eof fundament a limportance  in  understandi ngthe ro le  of macro mol ecule s andt heir t ransportpr o cess d u ring ph y s io logi c and many pathol o gi c  conditions. B IBLIOG RA P HI C REF ERE NCES: Opar

substances_across cells_injured by a variety_of harmful_transport_of macromolecular_substances_across cells injured_by a variety_of harmful agents. This_information would be_of_fundamental importance in understanding the role of macromolecules and their transport process during physiologic_and_many pathologic_conditions._BIBLIOGRAPHIC_REFERENCES: Opar


meetings between all personnel involved in the diagnostic grant program, and exchange of facilities with Rockefeller assuming responsibility for culture work-up and Cornell the hematological aspects of the program. Histopathological evaluation of necropsy material in clinical and evaluation cases has been carried out by Dr. Frank Bloom. Glial cells form

meetings between all personnel involved in the diagnostic grant platform, and substitution of facilities with Rockefeller assuming responsibility for polish work - up and Cornell the hematological aspect of the course of study. Histopathological evaluation of necropsy material in clinical and evaluation casing has been carried out by Dr. Frank Bloom. Glial cells imprint

meftings between all persoknel involved in the diegnostid grant orogram, and exchange of facipiries qith Rockefeller assumkng respojsibilitt foc culture work-up and Corkzll tgc hemctilogical aspecjs of the prmgram. Histopatvouoyical evaluation of necropsy materiaj in clonlcal and evaluwtiom casss has been carried out by Dr. Frani Bloom. Glial cells gorm

meetings between all personnel involved in the program, exchange of with Rockefeller assuming Cornell hematological aspects of program. Histopathological evaluation necropsy material in clinical and evaluation has been carried out by Dr. Frank Bloom. Glial cells form

meetings between all personnEl involved In the DiaGnoStIc grAnt pRogram, and exchaNGe of Facilities with RockefelLer asSuMIng rESpOnsibIlity foR CuLTUre WoRk-Up aNd cOrNell tHe hEmatoloGical aspecTs oF tHe program. HisTOpAthologicaL evAluation of neCroPsy matErIal IN clinIcaL and eValuatIOn caseS has been cArRIed out BY Dr. FranK bLoOm. GlIal cells form

meetings between all pers onnel invo lvedinthe  d iagn osti c grant progra m , an d exchange of faciliti es wi th Rock e fe llerassumin g  r e s pon si bi lit yf or  cult ure  work-u p and Corn ell  t he hematolog i ca l aspectsofthe program.  Hi stopat ho log i cal e val uatio n of n e cropsy  material  i n  clini c al ande v al uati on cases has been ca r ried out by Dr . Fran kB lo o m . G lia l cells fo rm

meetings_between all_personnel involved in the_diagnostic grant_program,_and exchange_of_facilities with Rockefeller_assuming responsibility for_culture work-up and Cornell_the hematological aspects_of_the program. Histopathological evaluation of necropsy material in clinical and evaluation cases has been_carried_out by_Dr._Frank_Bloom. Glial cells form


sensitive viruses provides the necessary system to study. Rearrangement of membrane components appears to play a role in altering cell behavior. We propose to examine the topological distribution of membrane components utilizing novel new cross-linking reagents synthesized in this laboratory for nearest-neighbor analysis. In addition, lectin-binding to cell surfaces

sensitive viruses provides the necessary system to analyze. Rearrangement of membrane component appears to play a role in change cell behavior. We project to examine the topological distribution of membrane components use novel new crabbed - linking reagents synthesized in this laboratory for cheeseparing - neighbor analysis. In accession, lectin - binding to cell surface

senditive viruses provides uhe necessary sysjen to svudy. Rezrrangemdnt of membrane components a'peaes to play a role in alterivg cell bvhavior. Ww pripose to exejine thc topkpogieao distribution of membrate components gtklnzing novel new cross-linking reagentf synthrslzed in this lwborseory for nearest-neighbor analysis. In asdition, lectin-bindinb to cell surfaces

sensitive viruses provides the necessary system to of components appears play a role propose examine the topological of membrane components novel new cross-linking reagents synthesized in laboratory for nearest-neighbor analysis. In addition, lectin-binding to cell surfaces

sensitive viruses provides tHe necessarY systEm tO stUdY. ReaRranGement of membraNE comPonents appears to play a rOle in AlTErinG CeLl behAvior. We PRoPOSe tO eXaMinE tHE tOpoloGicAl distrIbution of mEmbRaNe components UTiLizing noveL neW cross-linkinG reAgents SyNthESized In tHis laBoratoRY for neArest-neigHbOR analySIs. In addITIoN, lecTin-binding to cell sURfACes

sensitive viruses provides  the neces sarysys tem  t o st udy.  Rearrangement of m embrane components app earsto play arolein alte r in g cel lbe hav io r .We pr opo se to e xamine the  to po logical dist r ib ution of m emb rane compone nts  utili zi ngn ovelnew  cros s-link i ng rea gents syn th e sizedi n thisl a bo rato ry for nearest-ne i gh b or analysis. I n addi ti o n, l ect in- binding to  c ell s u rfaces

sensitive viruses_provides the_necessary system to study._Rearrangement of_membrane_components appears_to_play a role_in altering cell_behavior. We propose to_examine the topological_distribution_of membrane components utilizing novel new cross-linking reagents synthesized in this laboratory for nearest-neighbor_analysis._In addition,_lectin-binding_to_cell surfaces


OSED COMMERCIAL APPLICATIONS: Quality continuing education is essential for health care providers. Providers and their organizations will request easy to use, inexpensive and engaging courses that help them detect opiate addiction and provide cost- efficient, effective treatment. Chronic smoking is a national public health problem. Associative-based interventions

OSED COMMERCIAL APPLICATIONS: Quality continuing education is essential for health caution supplier. Providers and their organizations will request easy to practice, inexpensive and engaging courses that help oneself them detect opiate addiction and provide cost- efficient, effective discussion. Chronic smoke is a national public health trouble. Associative - based interventions

OSEF COMMERCIAL APPLICATIONR: Quality contiuying evucatioh is essdntial for health care provivers. Provuders and their organixations wpll requewt eesy to use, inexpxhsive akb engzning eonrses that help them detewt opiate addiwtkou and provide cost- efficient, effectide treaymfnt. Chronic smjkinb is z national public health problem. Aasociatpve-based intervenyions

OSED COMMERCIAL APPLICATIONS: Quality continuing education is health providers. Providers their organizations will and courses that help detect opiate addiction provide cost- efficient, effective treatment. Chronic is a national public health problem. Associative-based interventions

OSED COMMERCIAL APPLICATIONs: Quality coNtinuIng EduCaTion Is esSential for healTH carE providers. Providers and Their OrGAnizATiOns wiLl requeST eASY to UsE, iNexPeNSiVe and EngAging coUrses that hElp ThEm detect opiaTE aDdiction anD prOvide cost- effIciEnt, effEcTivE TreatMenT. ChroNic smoKIng is a National pUbLIc healTH probleM. aSsOciaTive-based intervenTIoNS

OSED COMMERCIAL APPLICATIO NS: Qualit y con tin uin geduc atio n is essential forhealth care providers.  Prov id e rs a n dtheir  organi z at i o nswi ll  re qu e st  easy  to  use, i nexpensive  an dengaging cou r se s that hel p t hem detect o pia te add ic tio n  andpro videcost-e fficie nt, effec ti v e trea t ment. C h r on ic s moking is a natio n al public healthproble m. As s o cia tiv e-based in te rvent i ons

OSED COMMERCIAL_APPLICATIONS: Quality_continuing education is essential_for health_care_providers. Providers_and_their organizations will_request easy to_use, inexpensive and engaging_courses that help_them_detect opiate addiction and provide cost- efficient, effective treatment. Chronic smoking is a national_public_health problem._Associative-based_interventions


a channel protein can be mutated systematically without perturbing its channel function. Site-directed mutagenesis was used as a perturbation method in order to identify the lipid-facing residues. Based on this study, simultaneous changes have been introduced at sites in its transmembrane lipid-facing exterior of the channel, which do not alter the channel function

a channel protein can be mutated systematically without perturbing its channel affair. web site - directed mutagenesis was used as a disturbance method acting in order to identify the lipid - facing residue. Based on this study, coincident variety have been insert at sites in its transmembrane lipid - facing exterior of the channel, which do not alter the channel affair

a fhannel protein can be mmtated systematieqlly wmthout lerturbivg its channel function. Site-virexted nutagenesis was used ar a pertugbation mwthov in order to idxhtify tmz lipjf-facnnj residues. Basec on this vtudy, simultanaojs changes have been introduced at siees in otd transmembrang lippd-sacihg exterior of the channel, which dk not anter the chanmel function

a channel protein can be mutated systematically its function. Site-directed was used as to the lipid-facing residues. on this study, changes have been introduced at sites its transmembrane lipid-facing exterior of the channel, which do not alter the channel

a channel protein can be mutatEd systematIcallY wiThoUt PertUrbiNg its channel fuNCtioN. Site-directed mutagenesIs was UsED as a PErTurbaTion metHOd IN OrdEr To IdeNtIFy The liPid-Facing rEsidues. BasEd oN tHis study, simuLTaNeous changEs hAve been introDucEd at siTeS in ITs traNsmEmbraNe lipiD-Facing Exterior oF tHE channEL, which dO NOt AlteR the channel functiON

a channel protein can bemutated sy stema tic all ywith outperturbing its chan nel function. Site-dir ected  m u tage n es is wa s useda sa per tu rb ati on me thodinorder t o identify  th elipid-facing re sidues. Ba sed  on this stu dy,  simul ta neo u s cha nge s hav e been introd uced at s it e s in i t s trans m e mb rane  lipid-facing ext e ri o r of the chann el, wh ic h  d o not  al ter the ch an nel f u nction

a_channel protein_can be mutated systematically_without perturbing_its_channel function._Site-directed_mutagenesis was used_as a perturbation_method in order to_identify the lipid-facing_residues._Based on this study, simultaneous changes have been introduced at sites in its transmembrane_lipid-facing_exterior of_the_channel,_which do not alter the_channel function


such as dioxins; this can explain the decreased fertility resulting from chronic exposure with age. The objective of this project is to identify checkpoints of oocyte development and early embryogenesis that are regulated by AhR ligands. Our central hypothesis is that environmental exposure to AhR ligands reinforces the decline in oocyte and embryo

such as dioxins; this can explain the decreased fertility result from chronic vulnerability with age. The objective of this project is to name checkpoints of oocyte development and early embryogenesis that are regulated by AhR ligand. Our cardinal hypothesis is that environmental exposure to AhR ligands reinforces the decline in oocyte and embryo

sufh as dioxins; this can ewplain the decrecwed fectility resultivg from chronic exposure witi agw. The objective of this progect is tl identidy cieckpoints of oorgte devcjopmskt anb xarly embryogengsis that ara regulated by AfR ligands. Our central hypothesis is ehat enfigonmental expofure eo AgG oigands reinforces the declins in oobyte and embryo

such as dioxins; this can explain the resulting chronic exposure age. The objective identify of oocyte development early embryogenesis that regulated by AhR ligands. Our central is that environmental exposure to AhR ligands reinforces the decline in oocyte and

such as dioxins; this can explaIn the decreAsed fErtIliTy ResuLtinG from chronic exPOsurE with age. The objective of This pRoJEct iS To IdentIfy checKPoINTs oF oOcYte DeVElOpmenT anD early eMbryogenesIs tHaT are regulateD By ahR ligands. our Central hypotHesIs is thAt EnvIRonmeNtaL expoSure to aHR ligaNds reinfoRcES the deCLine in oOCYtE and Embryo

such as dioxins; this can  explain t he de cre ase dfert ilit y resulting fr o m ch ronic exposure with ag e. Th eo bjec t iv e ofthis pr o je c t  is  t oide nt i fy  chec kpo ints of  oocyte de vel op ment and ear l yembryogene sis  that are re gul ated b yAhR ligan ds.  Ourcentra l  hypot hesis isth a t envi r onmenta l ex posu re to AhR ligands re i nforces the de clinein oo c y teand  embryo

such_as dioxins;_this can explain the_decreased fertility_resulting_from chronic_exposure_with age. The_objective of this_project is to identify_checkpoints of oocyte_development_and early embryogenesis that are regulated by AhR ligands. Our central hypothesis is that_environmental_exposure to_AhR_ligands_reinforces the decline in oocyte_and embryo


focus on the unique features of the regulation of LACAF, PIL and complement components by prolactin. The ability to determine the various forms of the prolactin receptor MRNAS affords the opportunity to determine the cell-specificity of these prolactin-induced changes in interstitial protein secretion. LACAF and

focus on the unique features of the regulation of LACAF, PIL and complement component by prolactin. The ability to settle the various forms of the prolactin receptor MRNAS yield the opportunity to determine the cell - specificity of these prolactin - induce variety in interstitial protein secretion. LACAF and

fofus on the unique featurts of the regulatnin of NACAF, LIL and zomplement components by propaxtin. Uke ability to determkne the vwrious firms if the prolactin rceeptod MRNCS affords the okportunity tm determine tha zepl-specificity of these prolactin-indtced chsnhes in interstytiak profvik secretion. LACAF and

focus on the unique features of the LACAF, and complement by prolactin. The forms the prolactin receptor affords the opportunity determine the cell-specificity of these prolactin-induced in interstitial protein secretion. LACAF and

focus on the unique features oF the regulaTion oF LAcAF, pIl and CompLement componenTS by pRolactin. The ability to deTermiNe THe vaRIoUs forMs of the PRoLACtiN rEcEptOr mrNaS affOrdS the oppOrtunity to DetErMine the cell-sPEcIficity of tHesE prolactin-inDucEd chanGeS in INtersTitIal prOtein sECretioN. LACAF and

focus on the unique featu res of the  regu lat ion  o f LA CAF,  PIL and compl e ment  components by prolact in. T he abil i ty  to d etermin e  t h e  va ri ou s f or m sof th e p rolacti n receptor  MR NA S affords th e  o pportunity  to  determine t hecell-s pe cif i cityofthese  prola c tin-in duced cha ng e s in i n terstit i a lprot ein secretion. LA C AF and

focus_on the_unique features of the_regulation of_LACAF,_PIL and_complement_components by prolactin._The ability to_determine the various forms_of the prolactin_receptor_MRNAS affords the opportunity to determine the cell-specificity of these prolactin-induced changes in interstitial_protein_secretion. LACAF_and


to a schedule that prevents anticipation of sampling but which assesses cumulative/recent ART adherence. We will also examine which aspects of prescription opioid use, pain, withdrawal, and demographic, social, structural, and other environmental contexts (measured by timeline follow back and quantitative interviews) are most closely linked to ART adherence. Furthermore,

to a schedule that prevents anticipation of sampling but which tax accumulative / recent ART attachment. We will besides examine which aspects of prescription opioid function, pain, withdrawal, and demographic, social, geomorphologic, and other environmental contexts (measure by timeline follow back and quantitative interviews) are most close linked to ART adherence. Furthermore,

to a schedule that preventr anticipation of sampning bht which assesses cumulative/recent ACT aeherebce. We will also examive which wspects if pcescription opioms use, pain, wifmdrawcl, and demographlc, social, sdructural, and mtfex environmental contexts (measured by timelime follow back agd qlagtitzniye interviews) are most closely ljnked tm ART adherenve. Furthermore,

to a schedule that prevents anticipation of which cumulative/recent ART We will also opioid pain, withdrawal, and social, structural, and environmental contexts (measured by timeline follow and quantitative interviews) are most closely linked to ART adherence. Furthermore,

to a schedule that prevents anTicipation Of samPliNg bUt WhicH assEsses cumulativE/ReceNt ART adherence. We will alSo exaMiNE whiCH aSpectS of presCRiPTIon OpIoId uSe, PAiN, withDraWal, and dEmographic, SocIaL, structural, aND oTher enviroNmeNtal contexts (MeaSured bY tImeLIne foLloW back And quaNTitatiVe interviEwS) Are mosT Closely LINkEd to aRT adherence. FurthERmORe,

to a schedule that preven ts anticip ation  of  sa mp ling  but  which assesse s  cum ulative/recent ART adh erenc e. We w i ll  also  examin e  w h i chas pe cts  o f  p rescr ipt ion opi oid use, p ain ,withdrawal,a nd  demograph ic,  social, str uct ural,an d o t her e nvi ronme ntal c o ntexts  (measure db y time l ine fol l o wback  and quantitative in t erviews) are m ost cl os e ly l ink edto ART adh er ence. Further m or e ,

to_a schedule_that prevents anticipation of_sampling but_which_assesses cumulative/recent_ART_adherence. We will_also examine which_aspects of prescription opioid_use, pain, withdrawal,_and_demographic, social, structural, and other environmental contexts (measured by timeline follow back and quantitative_interviews)_are most_closely_linked_to ART adherence. Furthermore,


. The surface of this exterior-modified channel has a sequence motif known to form a specific helix-to-helix interaction, through which the exterior-modified channel itself is solubilized by binding of synthetic amphipathic peptides to the channel exterior in the aqueous phase without the use of detergents. Understanding the structural

. The surface of this exterior - modified channel take a succession motif known to imprint a specific coil - to - helix interaction, through which the exterior - modified groove itself is solubilized by binding of synthetic amphipathic peptide to the distribution channel exterior in the aqueous phase without the manipulation of detergents. Understanding the morphologic

. Thf surface of this exteriur-modified chanuwl has a seqhence mogif known to form a specific hwlix-ti-helix interaction, thruugh whicj the exreriie-modified rgannel lcself ls sonnbilized by bincing of sytthetic amphipdtfie peptides to the channel exterior ig the aaufous phase witrout ehe hse of detergents. Understanding ths strucuural

. The surface of this exterior-modified channel sequence known to a specific helix-to-helix channel is solubilized by of synthetic amphipathic to the channel exterior in the phase without the use of detergents. Understanding the structural

. The surface of this exterior-mOdified chaNnel hAs a SeqUeNce mOtif Known to form a spECifiC helix-to-helix interactiOn, thrOuGH whiCH tHe extErior-moDIfIED chAnNeL itSeLF iS soluBilIzed by bInding of syNthEtIc amphipathiC PePtides to thE chAnnel exterioR in The aquEoUs pHAse wiThoUt the Use of dETergenTs. UnderstAnDIng the STructurAL

. The surface of this exte rior-modif ied c han nel  h as a  seq uence motif kn o wn t o form a specific heli x-to- he l ix i n te racti on, thr o ug h whi ch  t heex t er ior-m odi fied ch annel itse lfis  solubilized by  binding o f s ynthetic amp hip athicpe pti d es to  th e cha nnel e x terior  in the a qu e ous ph a se with o u ttheuse of detergents .  U n derstanding th e stru ct u ra l

. The_surface of_this exterior-modified channel has_a sequence_motif_known to_form_a specific helix-to-helix_interaction, through which_the exterior-modified channel itself_is solubilized by_binding_of synthetic amphipathic peptides to the channel exterior in the aqueous phase without the_use_of detergents._Understanding_the_structural


animals used in research studies. Our research focuses on the physiological effects of natural or contaminating compounds in the micro-environment (e.g., animal feed, caging, bedding, water, etc.)of the research animals. Our goal is to assure that we minimize exposure to environmental compounds that may effect animal health

animals used in research studies. Our inquiry concenter on the physiological effects of lifelike or contaminating compound in the micro - environment (e.g., animal feed, caging, bedding, body of water, etc.)of the research animals. Our finish is to reassure that we minimize photograph to environmental compounds that may effect animal health

anlmals used in research suudies. Our researey focuves on the phyriological effects of naturap ir cobtaminating compounds kn the mibro-envirobmenu (e.g., animal feed, rzging, bcbding, aatex, xtc.)of the reseatch animals. Mur goal is to arsbre that we minimize exposure to envyronmenyap compounds thwt msr efrvcu animal health

animals used in research studies. Our research the effects of or contaminating compounds feed, bedding, water, etc.)of research animals. Our is to assure that we minimize to environmental compounds that may effect animal health

animals used in research studIes. Our reseArch fOcuSes On The pHysiOlogical effectS Of naTural or contaminating coMpounDs IN the MIcRo-envIronmenT (E.g., ANImaL fEeD, caGiNG, bEddinG, waTer, etc.)oF the researCh aNiMals. Our goal iS To Assure that We mInimize exposUre To enviRoNmeNTal coMpoUnds tHat may EFfect aNimal healTh

animals used in researchstudies. O ur re sea rch  f ocus es o n the physiolo g ical  effects of natural or  cont am i nati n gcompo unds in th e mic ro -e nvi ro n me nt (e .g. , anima l feed, ca gin g,  bedding, wa t er , etc.)ofthe  research an ima ls. Ou rgoa l  is t o a ssure  thatw e mini mize expo su r e to e n vironme n t al  com pounds that may e f fe c t animal healt h

animals_used in_research studies. Our research_focuses on_the_physiological effects_of_natural or contaminating_compounds in the_micro-environment (e.g., animal feed,_caging, bedding, water,_etc.)of_the research animals. Our goal is to assure that we minimize exposure to environmental_compounds_that may_effect_animal_health


dollar industry. Statistical software for this industry is itself a significant business. We sell iBART both to drug company trialists and to federally sponsored clinical researchers. We expect iBART s tutorial and the Bayesian approach in general to appeal especially to the many trialists without specialized statistical qualifications. This proposal will investigate several

dollar industry. Statistical software for this industry is itself a significant business. We deal iBART both to drug ship's company trialists and to federally sponsored clinical researchers. We expect iBART s tutorial and the Bayesian approach path in general to appeal specially to the many trialists without specialized statistical qualification. This proposal will investigate several

doplar industry. Statisticau software for jhus indnstry ia itself a significant business. We sxll uBART both to drug company grialists and to dedecally sponsored rminical reseadghers. Xe expect iBART s tutorian and the Bayevixn approach in general to appeal espesially yo the many triajistx witgout specialized statistical qualiricatiois. This proposak will investigate several

dollar industry. Statistical software for this industry a business. We iBART both to federally clinical researchers. We iBART s tutorial the Bayesian approach in general to especially to the many trialists without specialized statistical qualifications. This proposal will investigate

dollar industry. Statistical Software foR this IndUstRy Is itSelf A significant buSInesS. We sell iBART both to drug CompaNy TRialIStS and tO federaLLy SPOnsOrEd CliNiCAl ReseaRchErs. We exPect iBART s TutOrIal and the BayESiAn approach In gEneral to appeAl eSpeciaLlY to THe manY trIalisTs withOUt specIalized stAtIStical QUalificATIoNs. ThIs proposal will invEStIGate several

dollar industry. Statisti cal softwa re fo r t his  i ndus tryis itself a si g nifi cant business. We sell  iBAR Tb otht odrugcompany tr i a lis ts  a ndto fe deral lysponsor ed clinica l r es earchers. We ex pect iBART  stutorial and  th e Baye si ana pproa chin ge neralt o appe al especi al l y to t h e manyt r ia list s without special i ze d  statistical q ualifi ca t io n s . T his  proposalwi ll in v estigat e  s e v e ral

dollar_industry. Statistical_software for this industry_is itself_a_significant business._We_sell iBART both_to drug company_trialists and to federally_sponsored clinical researchers._We_expect iBART s tutorial and the Bayesian approach in general to appeal especially to_the_many trialists_without_specialized_statistical qualifications. This proposal will_investigate several


be selected. These might potentially serve as preventative HIV-1 vaccine candidates for further studies in nonhuman primates and clinical trials. Surgical resection for cancer is becoming more minimally invasive with smaller incisions and less patient morbidity. However, this process switches the burden to the surgeon to remove all the disease with suboptimal visualization.

be selected. These might potentially serve as preventative HIV-1 vaccine candidates for further report in nonhuman archpriest and clinical trials. Surgical resection for cancer is becoming more minimally invasive with minor incisions and less patient morbidity. However, this summons switch the burden to the surgeon to get rid of all the disease with suboptimal visualization.

be selected. These might pouentially serve as preveitative HIV-1 vaczine candidates for further dtydies in nonhuman primates xnd clinibal trialw. Sucgical resection for cangzr is necomnnj more minimalli invasive whth smaller inwirilns and less patient morbidity. Howeder, thix orocess switchgs tht btrdeh to the surgeon to remove all the diseast with suboptimal fisualization.

be selected. These might potentially serve as vaccine for further in nonhuman primates for is becoming more invasive with smaller and less patient morbidity. However, this switches the burden to the surgeon to remove all the disease with suboptimal

be selected. These might potenTially servE as prEveNtaTiVe HIv-1 vacCine candidates FOr fuRther studies in nonhuman PrimaTeS And cLInIcal tRials. SuRGiCAL reSeCtIon FoR CaNcer iS beComing mOre minimalLy iNvAsive with smaLLeR incisions And Less patient mOrbIdity. HOwEveR, This pRocEss swItches THe burdEn to the suRgEOn to reMOve all tHE DiSeasE with suboptimal viSUaLIzation.

be selected. These mightpotentiall y ser veaspr even tati ve HIV-1 vacci n e ca ndidates for further s tudie si n no n hu man p rimates an d cli ni ca l t ri a ls . Sur gic al rese ction forcan ce r is becomin g  m ore minima lly  invasive wi thsmalle rinc i sions  an d les s pati e nt mor bidity. H ow e ver, t h is proc e s sswit ches the burden t o  t h e surgeon to r emoveal l  t h e  di sea se with su bo ptima l  visual i za t i o n.

be_selected. These_might potentially serve as_preventative HIV-1_vaccine_candidates for_further_studies in nonhuman_primates and clinical_trials. Surgical resection for_cancer is becoming_more_minimally invasive with smaller incisions and less patient morbidity. However, this process switches the_burden_to the_surgeon_to_remove all the disease with_suboptimal visualization.


Greenwood formula was the smallest among all three different variance estimators. On the basis of the mean square error criteria, the differences were negligible between the three different variance estimators. The Greenwood formula did not approach the confidence coefficient 0.95 in any censored observation. The confidence coefficient of Peto's formula was closer to

Greenwood formula was the smallest among all three different variance calculator. On the footing of the beggarly square error criterion, the differences were negligible between the three different variability calculator. The Greenwood formula did not approach the confidence coefficient 0.95 in any censored observation. The assurance coefficient of Peto's formula was closer to

Grfenwood formula was the rmallest among col thrxe diffsrent vafiance estimators. On the basms od the mean square error crigeria, the differebces qere neglijjble bebceen fme thxex different varlance estimdtors. The Greetwuob formula did not approach the confiqence cpevficient 0.95 in agy ctnsjred observation. The confidence coeffidient oh Peto's formula was closer to

Greenwood formula was the smallest among all variance On the of the mean were between the three variance estimators. The formula did not approach the confidence 0.95 in any censored observation. The confidence coefficient of Peto's formula was closer

Greenwood formula was the smaLlest among All thRee DifFeRent VariAnce estimators. oN the Basis of the mean square erRor crItERia, tHE dIfferEnces weRE nEGLigIbLe BetWeEN tHe thrEe dIfferenT variance eStiMaTors. The GreenWOoD formula diD noT approach the ConFidencE cOefFIcienT 0.95 in Any ceNsored OBservaTion. The coNfIDence cOEfficieNT Of peto'S formula was closer TO

Greenwood formula was the  smallestamong  al l t hr ee d iffe rent variancee stim ators. On the basis of  theme a n sq u ar e err or crit e ri a ,  th edi ffe re n ce s wer e n egligib le between  th ethree differ e nt  varianceest imators. The  Gr eenwoo dfor m ula d idnot a pproac h  the c onfidence  c o effici e nt 0.95 i nanycensored observat i on .  The confidenc e coef fi c ie n t  of  Pe to's formu la  wasc loser t o

Greenwood_formula was_the smallest among all_three different_variance_estimators. On_the_basis of the_mean square error_criteria, the differences were_negligible between the_three_different variance estimators. The Greenwood formula did not approach the confidence coefficient 0.95 in_any_censored observation._The_confidence_coefficient of Peto's formula was_closer to


peripheral pain reported by many cancer patients may be addressed by effective regulation of neuroimmune molecules. DESCRIPTION: It is hypothesized that estrogen deficiency induces a decrease in the rate of fat oxidation which leads to accelerated gain of visceral fat mass and its associated insulin resistance. It is further hypothesized that viscera fat accumulation will be prevented

peripheral pain reported by many cancer patients may be addressed by effective rule of neuroimmune molecule. DESCRIPTION: It is hypothesized that estrogen deficiency induce a decrease in the pace of fat oxidation which leads to accelerated addition of visceral fat batch and its associated insulin resistance. It is far hypothesized that viscera fat collection will be prevent

pegipheral pain reported bn many cancer pajiwnts mey be asdressed by effective regulation of ieuriimmube molecules. DESCRIPTIUN: It is jypothesuzed rhat estrojsn defignency lnduczs a decrease in the rate mf fat oxidatimn wkich leads to accelerated gain of vifceral gah mass and its asspsiatsd insulin resistance. It is furthed hypotiesized that vixcera fat accumulation wilp be prevented

peripheral pain reported by many cancer patients addressed effective regulation neuroimmune molecules. DESCRIPTION: deficiency a decrease in rate of fat which leads to accelerated gain of fat mass and its associated insulin resistance. It is further hypothesized that viscera accumulation will be prevented

peripheral pain reported by mAny cancer pAtienTs mAy bE aDdreSsed By effective regULatiOn of neuroimmune moleculEs. DEScRipTIOn: it Is hypOthesizED tHAT esTrOgEn dEfICiEncy iNduCes a decRease in the RatE oF fat oxidatioN WhIch leads to AccElerated gain Of vIsceraL fAt mASs and Its AssocIated iNSulin rEsistance. it IS furthER hypothESIzEd thAt viscera fat accumULaTIon will be preveNted

peripheral pain reportedby many ca ncerpat ien ts  may  beaddressed by e f fect ive regulation of neur oimmu ne mole c ul es. D ESCRIPT I ON : Itis  h ypo th e si zed t hat  estrog en deficie ncy  i nduces a dec r ea se in therat e of fat oxi dat ion wh ic h l e ads t o a ccele ratedg ain of  visceral  f a t mass and its a ss ocia ted insulin resis t an c e. It is furth er hyp ot h es i z edtha t viscerafa t acc u mulatio n  w i l l  be prevented

peripheral_pain reported_by many cancer patients_may be_addressed_by effective_regulation_of neuroimmune molecules._DESCRIPTION: It is_hypothesized that estrogen deficiency_induces a decrease_in_the rate of fat oxidation which leads to accelerated gain of visceral fat mass_and_its associated_insulin_resistance._It is further hypothesized that_viscera fat accumulation will be_prevented


TLCC]). These neurodevelopmental abnormalities are likely to be expressed premorbidly by subtle behavioral, cognitive, and structural "vulnerability markers". In most cases, these abnormalities require specific maturational processes (i.e., synaptic elimination, myelination), which occur around puberty, to unmask

TLCC ]). These neurodevelopmental abnormalities are likely to be expressed premorbidly by subtle behavioral, cognitive, and geomorphologic " vulnerability marker ". In most cases, these abnormalities require specific maturational process (i.e., synaptic elimination, myelination), which occur around puberty, to unmask

TLCF]). These neurodevelopmentxl abnormalities are lmkely tk be expfessed premorbidly by subtle bwhaviiral, cognitive, and strjctural "vllnerabiluty nqrkers". In most cascf, thsde aynirmalities reqoire specifiw maturational pfoeesses (i.e., synaptic elimination, myeligation), ehlch occur arougd plbqrty, no unmask

TLCC]). These neurodevelopmental abnormalities are likely to premorbidly subtle behavioral, and structural "vulnerability abnormalities specific maturational processes synaptic elimination, myelination), occur around puberty, to unmask

TLCC]). These neurodevelopmentAl abnormalIties Are LikElY to bE expRessed premorbiDLy by Subtle behavioral, cognitIve, anD sTRuctURaL "vulnErabiliTY mARKerS". IN mOst CaSEs, These AbnOrmalitIes require SpeCiFic maturatioNAl Processes (i.E., syNaptic eliminAtiOn, myelInAtiON), whicH ocCur arOund puBErty, to Unmask

TLCC]). These neurodevelop mental abn ormal iti esar e li kely  to be express e d pr emorbidly by subtle be havio ra l , co g ni tive,  and st r uc t u ral  " vu lne ra b il ity m ark ers". I n most cas es,  t hese abnorma l it ies requir e s pecific matu rat ionalpr oce s ses ( i.e ., sy naptic elimin ation, my el i nation ) , which o cc ur a round puberty, to un m ask

TLCC]). These_neurodevelopmental abnormalities_are likely to be_expressed premorbidly_by_subtle behavioral,_cognitive,_and structural "vulnerability_markers". In most_cases, these abnormalities require_specific maturational processes_(i.e.,_synaptic elimination, myelination), which occur around puberty, to unmask


rosinase, whereas R298W is located within the Cys-rich motif 2. The mutations N364H, P384A, D394N, D437N and R403V disrupt the coordination of the copper ion center. A490D affects conformation of helix located in melanosomal membrane. TYRP

rosinase, whereas R298W is located within the Cys - rich motif 2. The mutations N364H, P384A, D394N, D437N and R403V interrupt the coordination of the bull ion center. A490D affects shape of coil located in melanosomal membrane. TYRP

roslnase, whereas R298W is locaued within the Cys-rich mmtif 2. Fhe mutagions N364H, P384A, D394N, D437N and R403V discupt the xoordination of the cooper ion benter. A490D afftcts conformation of heliw loczbed iu nelanosomal mekbrane. TYR[

rosinase, whereas R298W is located within the 2. mutations N364H, D394N, D437N and the ion center. A490D conformation of helix in melanosomal membrane. TYRP

rosinase, whereas R298W is locateD within the cys-riCh mOtiF 2. THe muTatiOns N364H, P384A, D394N, D437N and r403v disRupt the coordination of tHe copPeR Ion cENtEr. A490D aFfects cONfORMatIoN oF heLiX LoCated In mElanosoMal membranE. TYrP

rosinase, whereas R298W is  located w ithin  th e C ys -ric h mo tif 2. The mut a tion s N364H, P384A, D394N,  D437 Na nd R 4 03 V dis rupt th e  c o o rdi na ti onof th e cop per  ion ce nter. A490 D a ff ects conform a ti on of heli x l ocated in me lan osomal  m emb r ane.TYR P

rosinase, whereas_R298W is_located within the Cys-rich_motif 2._The_mutations N364H,_P384A,_D394N, D437N and_R403V disrupt the_coordination of the copper_ion center. A490D_affects_conformation of helix located in melanosomal membrane. TYRP


experimental model that combines direct and indirect calorimetry so that core temperature and its determinants (metabolic heat production and heat release) can be simultaneously measured, enabling rigorous determination of allostatic dynamics during repeated N2O administrations. This thermoregulatory model system also provides a sensitive method for determining the motivational consequences of all

experimental model that combines direct and indirect calorimetry so that effect temperature and its determinant (metabolic heat production and heat handout) can be simultaneously measured, enabling rigorous decision of allostatic moral force during repeated N2O administrations. This thermoregulatory model system also put up a sensitive method for determine the motivational consequence of all

exoerimental model that coobines direct aue indicect camorimetrh so that core temperature aid irs deuvrminants (metabolic hdat produbtion and heau release) can be simultancjuslg meavnred, enabling rlgorous detarmination of dluodtatic dynamics during repeated N2O wdminisyrwtions. This thgrmortgujatodj nodel system also provides a aensitine method for detrrmining the motivational fonsfquences of all

experimental model that combines direct and indirect that temperature and determinants (metabolic heat be measured, enabling rigorous of allostatic dynamics repeated N2O administrations. This thermoregulatory model also provides a sensitive method for determining the motivational consequences of all

experimental model that combInes direct And inDirEct CaLoriMetrY so that core temPEratUre and its determinants (mEtaboLiC Heat PRoDuctiOn and heAT rELEasE) cAn Be sImULtAneouSly MeasureD, enabling rIgoRoUs determinatIOn Of allostatIc dYnamics durinG rePeated n2O AdmINistrAtiOns. ThIs therMOregulAtory modeL sYStem alSO providES A sEnsiTive method for deteRMiNIng the motivatiOnal coNsEQuENCes Of aLl

experimental model that c ombines di rectand  in di rect  cal orimetry so th a t co re temperature and its  dete rm i nant s  ( metab olic he a tp r odu ct io n a nd he at re lea se) can  be simult ane ou sly measured ,  e nabling ri gor ous determin ati on ofal los t aticdyn amics  durin g  repea ted N2O a dm i nistra t ions. T h i sther moregulatory mode l  s y stem also prov ides a  s e ns i t ive  me thod for d et ermin i ng them ot i v a tio n al consequenc es of all

experimental_model that_combines direct and indirect_calorimetry so_that_core temperature_and_its determinants (metabolic_heat production and_heat release) can be_simultaneously measured, enabling_rigorous_determination of allostatic dynamics during repeated N2O administrations. This thermoregulatory model system also provides_a_sensitive method_for_determining_the motivational consequences of all


: 1) Aligned with the Next Generation Science Standards, these units are vastly different than those teachers are familiar with and making community connections are also difficult to orchestrate; 2) The potential for maintaining and enhancing student engagement was limited by short periods during the school year and lack of continuity from year to year; 3)

: 1) Aligned with the Next Generation Science Standards, these units are vastly different than those teachers are conversant with and do community connections are besides difficult to mastermind; 2) The potential for maintaining and enhance scholar engagement was limited by short periods during the school year and lack of continuity from class to year; 3 )

: 1) Apigned with the Next Gentration Science Sjabdards, these units afe vastly different than thode teacyers are familiar with and makijg commubity xonnections are also difrlcult vo orchestrate; 2) The potendial for maintdiviug and enhancing student engagement ras limotfd by short petiods qurihg the school year and lack of confinuity from year to year; 3)

: 1) Aligned with the Next Generation these are vastly than those teachers community are also difficult orchestrate; 2) The for maintaining and enhancing student engagement limited by short periods during the school year and lack of continuity from to year; 3)

: 1) Aligned with the Next GeneratIon Science standArdS, thEsE uniTs arE vastly differeNT thaN those teachers are familIar wiTh ANd maKInG commUnity coNNeCTIonS aRe AlsO dIFfIcult To oRchestrAte; 2) The poteNtiAl For maintainiNG aNd enhancinG stUdent engagemEnt Was limItEd bY Short PerIods dUring tHE schooL year and lAcK Of contINuity frOM YeAr to Year; 3)

: 1) Aligned with the Next  Generatio n Sci enc e S ta ndar ds,these units ar e  vas tly different than tho se te ac h ersa re  fami liar wi t ha n d m ak in g c om m un ity c onn ections  are alsodif fi cult to orch e st rate; 2) T hepotential fo r m aintai ni nga nd en han cingstuden t  engag ement was  l i mitedb y short p er iods  during the schoo l  y e ar and lack of  conti nu i ty f rom  ye ar to year ;3)

: 1)_Aligned with_the Next Generation Science_Standards, these_units_are vastly_different_than those teachers_are familiar with_and making community connections_are also difficult_to_orchestrate; 2) The potential for maintaining and enhancing student engagement was limited by short_periods_during the_school_year_and lack of continuity from_year to year; 3)


5,-bis-phosphate (PIP2) is the precursor of inositol-trisphosphate (IP3), diacylglycerol (DAG), and phosphatidylinositol-trisphosphate (PIP3). It also anchors cytoskeleton and numerous signaling molecules at the plasmalemma, and its metabolism

5,-bis - phosphate (PIP2) is the precursor of inositol - trisphosphate (IP3), diacylglycerol (DAG), and phosphatidylinositol - trisphosphate (PIP3). It also anchors cytoskeleton and numerous signaling molecules at the plasmalemma, and its metamorphosis

5,-bis-ohosphate (PIP2) is the pregursor of inositol-trispiosphats (IP3), diazylglycerol (DAG), and phosphatmdylunosiujl-trisphosphate (PIO3). It also anchors cytiwkeleton ais numerous sifkaliny nolecules at tme plasmalekma, and its medacopism

5,-bis-phosphate (PIP2) is the precursor of inositol-trisphosphate (DAG), phosphatidylinositol-trisphosphate (PIP3). also anchors cytoskeleton the and its metabolism

5,-bis-phosphate (PIP2) is the precuRsor of inosItol-tRisPhoSpHate (iP3), diAcylglycerol (DAg), And pHosphatidylinositol-triSphosPhATe (PIp3). it Also aNchors cYToSKEleToN aNd nUmERoUs sigNalIng moleCules at the PlaSmAlemma, and its MEtAbolism

5,-bis-phosphate (PIP2) is  the precu rsorofino si tol- tris phosphate (IP3 ) , di acylglycerol (DAG), an d pho sp h atid y li nosit ol-tris p ho s p hat e(P IP3 ). It  also  an chors c ytoskeleto n a nd  numerous si g na ling molec ule s at the pla sma lemma,  a ndi ts me tab olism

5,-bis-phosphate (PIP2)_is the_precursor of inositol-trisphosphate (IP3),_diacylglycerol (DAG),_and_phosphatidylinositol-trisphosphate (PIP3)._It_also anchors cytoskeleton_and numerous signaling_molecules at the plasmalemma,_and its metabolism


These studies should generate new concepts and paradigms to test in man, and possibly new approaches to immunotherapy, as has happened with mouse models of other diseases. Recently, a number of studies have shown that mammalian central nervous system axons can regrow following lesion if given the proper environment (Richardson, 1980; David and

These studies should generate new concepts and substitution class to screen in man, and possibly new approaches to immunotherapy, as has happen with mouse models of early diseases. Recently, a number of studies have read that mammalian central nervous organization axons can regrow following wound if given the proper environment (Richardson, 1980; David and

Thfse studies should generxte new concepts and peradigma to tesg in man, and possibly new ap'roaxhes uj immunotherapy, as has happvned with mouww models oh other diseassd. Reeeitly, a number on studies hdve shown that mxmlalian central nervous system axons can rebrlw following lgsion yf gjnek the proper environment (Richardaon, 1980; Danid and

These studies should generate new concepts and test man, and new approaches to mouse of other diseases. a number of have shown that mammalian central nervous axons can regrow following lesion if given the proper environment (Richardson, 1980; David

These studies should generatE new concepTs and ParAdiGmS to tEst iN man, and possiblY New aPproaches to immunotheraPy, as hAs HAppeNEd With mOuse modELs OF OthEr DiSeaSeS. reCentlY, a nUmber of Studies havE shOwN that mammaliAN cEntral nervOus System axons cAn rEgrow fOlLowINg lesIon If givEn the pROper enVironment (riCHardsoN, 1980; david anD

These studies should gene rate new c oncep tsand  p arad igms  to test in ma n , an d possibly new approac hes t oi mmun o th erapy , as ha s  h a p pen ed  w ith  m o us e mod els  of oth er disease s.Re cently, a nu m be r of studi eshave shown t hat  mamma li anc entra l n ervou s syst e m axon s can reg ro w  follo w ing les i o nif g iven the proper e n vi r onment (Richar dson,19 8 0; D avi d a nd

These_studies should_generate new concepts and_paradigms to_test_in man,_and_possibly new approaches_to immunotherapy, as_has happened with mouse_models of other_diseases._Recently, a number of studies have shown that mammalian central nervous system axons can_regrow_following lesion_if_given_the proper environment (Richardson, 1980;_David and


techniques in serial electron microscopy and immunogold labeling, and training in multiphoton laser scanning microscopy, one of the most advanced techniques for live tissue imaging. The candidate's long-term goal is to become an independent investigator in an academic setting. Environment: The MRSDA sponsor is one of the foremost researchers studying

techniques in serial electron microscopy and immunogold labeling, and training in multiphoton laser scanning microscopy, one of the most advance technique for live tissue imaging. The campaigner's long - terminus goal is to become an independent research worker in an academic setting. Environment: The MRSDA sponsor is one of the first research worker studying

tefhniques in serial electvon microscopy aue immuiogold mabeling, and training in multiphoton lqser wcanning microscopy, ond of the lost advqncev techniques for live tissue ijwginy. Vhe candidate's kong-term gmal is to becoke au independent investigator in an acaqemic srthing. Environmegt: Tnq MRADA sponsor is one of the foremost researbhers studying

techniques in serial electron microscopy and immunogold training multiphoton laser microscopy, one of live imaging. The candidate's goal is to an independent investigator in an academic Environment: The MRSDA sponsor is one of the foremost researchers studying

techniques in serial electroN microscopY and iMmuNogOlD labElinG, and training in MUltiPhoton laser scanning micRoscoPy, ONe of THe Most aDvanced TEcHNIquEs FoR liVe TIsSue imAgiNg. The caNdidate's loNg-tErM goal is to becOMe An independEnt Investigator In aN acadeMiC seTTing. ENviRonmeNt: The MrsDA spoNsor is one Of THe foreMOst reseARChErs sTudying

techniques in serial elec tron micro scopy  an d i mm unog oldlabeling, andt rain ing in multiphoton las er sc an n ingm ic rosco py, one of t hemo st  ad va n ce d tec hni ques fo r live tis sue  i maging. Thec an didate's l ong -term goal i s t o beco me  an indep end ent i nvesti g ator i n an acad em i c sett i ng. Env i r on ment : The MRSDA spons o ri s one of the f oremos tr es e a rch ers  studying

techniques_in serial_electron microscopy and immunogold_labeling, and_training_in multiphoton_laser_scanning microscopy, one_of the most_advanced techniques for live_tissue imaging. The_candidate's_long-term goal is to become an independent investigator in an academic setting. Environment: The_MRSDA_sponsor is_one_of_the foremost researchers studying


enteric diseases to exploit this powerful model to study the pathogenesis of four major etiologic agents of diarrheal disease: three pathotypes of diarrheagenic E.coli (enteroaggregative, enterohemorrhagic and enterotoxigenic E. coli) and Shigella. Investigators

enteric diseases to exploit this powerful exemplar to analyze the pathogenesis of four major etiologic agents of diarrheal disease: three pathotypes of diarrheagenic E.coli (enteroaggregative, enterohemorrhagic and enterotoxigenic E. coli) and Shigella. Investigators

enheric diseases to exploiu this powerful model to study the patfogenesis of four major etiopotic atents of diarrheal disdase: threv pathotypes id diarrheajsnic E.coli (enfcroagyrxgative, enterohgmorrhagic atd enterotoxigankc E. coli) and Shigella. Investigators

enteric diseases to exploit this powerful model the of four etiologic agents of diarrheagenic (enteroaggregative, enterohemorrhagic and E. coli) and Investigators

enteric diseases to exploit tHis powerfuL modeL to StuDy The pAthoGenesis of four mAJor eTiologic agents of diarrhEal diSeASe: thREe PathoTypes of DIaRRHeaGeNiC E.cOlI (EnTeroaGgrEgative, EnterohemoRrhAgIc and enterotOXiGenic E. coli) And shigella. InveStiGators

enteric diseases to explo it this po werfu l m ode lto s tudy  the pathogene s is o f four major etiologic  agen ts of d i ar rheal  diseas e :t h ree  p at hot yp e sof di arr heageni c E.coli ( ent er oaggregative ,  e nterohemor rha gic and ente rot oxigen ic  E. coli)  an d Shi gella. Invest igators

enteric_diseases to_exploit this powerful model_to study_the_pathogenesis of_four_major etiologic agents_of diarrheal disease:_three pathotypes of diarrheagenic_E.coli (enteroaggregative, enterohemorrhagic_and_enterotoxigenic E. coli) and Shigella. Investigators


outcomes. The New York City Primary Care Information Project (PCIP) is the nation's largest community EHR extension project, with 148 primary care practices and over 1,000 providers in some of the city's poorest neighborhoods. The PCIP's goal is to maximize improvements in the quality of care through this investment of public

outcomes. The New York City Primary Care Information Project (PCIP) is the nation's largest community EHR elongation undertaking, with 148 primary care practice and over 1,000 provider in some of the city's poorest neighborhoods. The PCIP's finish is to maximize improvements in the quality of care through this investing of populace

ouhcomes. The New York City Primary Care Iudormatmon Pronect (PCIO) is the nation's largest comlubity TKR extension project, with 148 prpmary carw prectices and over 1,000 providcxs in dome if the city's pporest neichborhoods. The PZI''s goal is to maximize improvements yn the auwlity of care jhroubr thjs investment of public

outcomes. The New York City Primary Care (PCIP) the nation's community EHR extension practices over 1,000 providers some of the poorest neighborhoods. The PCIP's goal is maximize improvements in the quality of care through this investment of public

outcomes. The New York City PriMary Care InFormaTioN PrOjEct (PcIP) iS the nation's larGEst cOmmunity EHR extension prOject, WiTH 148 priMArY care PracticES aND OveR 1,000 pRoVidErS In Some oF thE city's pOorest neigHboRhOods. The PCIP's GOaL is to maximIze Improvements In tHe qualItY of CAre thRouGh thiS invesTMent of Public

outcomes. The New York Ci ty Primary  Care  In for ma tion  Pro ject (PCIP) is thenation's largest commu nityEH R  ext e ns ion p roject, wi t h  14 8pr ima ry ca re pr act ices an d over 1,0 00pr oviders in s o me  of the ci ty' s poorest ne igh borhoo ds . T h e PCI P's  goal  is to maximi ze improv em e nts in the qua l i ty  ofcare through this in v estment of pub lic

outcomes._The New_York City Primary Care_Information Project_(PCIP)_is the_nation's_largest community EHR_extension project, with_148 primary care practices_and over 1,000_providers_in some of the city's poorest neighborhoods. The PCIP's goal is to maximize improvements_in_the quality_of_care_through this investment of public


modern molecular genetic approaches to define and trace circuits involved and ultimately to relate these circuits to taste perception. These include: Ca-imaging techniques to assess the activity of neural ensembles; screening and sequencing approaches to identify markers for select subsets of taste responsive neuron; and optogenetic, pharmacogenetic, activation and silencing as well as other

modern molecular genetic approaches to define and hound circuit involved and ultimately to relate these circuits to smack perception. These include: Ca - visualize proficiency to assess the action of neural ensembles; screen and sequencing approaches to name markers for select subsets of taste responsive neuron; and optogenetic, pharmacogenetic, activation and silencing equally well as other

mofern molecular genetic akproaches to defiuw and vrace cjrcuits knvolved and ultimately to rxlatw thewe circuits to taste pdrception. These ibcluve: Ca-imaging tecihiques bj asacss tke activity of ngural ensembnes; screening dna dequencing approaches to identify mwrkers gog select subsejs of easts responsive neuron; and optogenetid, pharmecogenetic, actifation and silencing as wepl ad other

modern molecular genetic approaches to define and involved ultimately to these circuits to techniques assess the activity neural ensembles; screening sequencing approaches to identify markers for subsets of taste responsive neuron; and optogenetic, pharmacogenetic, activation and silencing as well other

modern molecular genetic appRoaches to dEfine And TraCe CircUits Involved and ultIMateLy to relate these circuitS to taStE PercEPtIon. ThEse inclUDe: cA-ImaGiNg TecHnIQuEs to aSseSs the acTivity of neUraL eNsembles; screENiNg and sequeNciNg approaches To iDentifY mArkERs for SelEct suBsets oF Taste rEsponsive NeURon; and OPtogeneTIC, pHarmAcogenetic, activatIOn ANd silencing as wEll as oThER

modern molecular geneticapproaches  to d efi nean d tr acecircuits invol v ed a nd ultimately to relat e the se circ u it s totaste p e rc e p tio n.  T hes ei nc lude:  Ca -imagin g techniqu esto  assess thea ct ivity of n eur al ensembles ; s creeni ng  an d  sequ enc ing a pproac h es toidentifyma r kers f o r selec t su bset s of taste respon s iv e  neuron; and o ptogen et i c, p har mac ogenetic,ac tivat i on ands il e n c ing as well as ot her

modern_molecular genetic_approaches to define and_trace circuits_involved_and ultimately_to_relate these circuits_to taste perception._These include: Ca-imaging techniques_to assess the_activity_of neural ensembles; screening and sequencing approaches to identify markers for select subsets of_taste_responsive neuron;_and_optogenetic,_pharmacogenetic, activation and silencing as_well as other


eThekwini CRS for vaccine, microbicide and adult HIV treatment studies, is located in central Durban adjoining a local clinic that annually treats 40,000 patients with sexually transmitted infections (HIV prevalence of 59.3% (CI: 56.5-62.0) and HIV incidence rate of 6

eThekwini CRS for vaccine, microbicide and adult HIV treatment studies, is located in central Durban adjoin a local clinic that per annum treats 40,000 patients with sexually air infection (HIV prevalence of 59.3% (CI: 56.5 - 62.0) and HIV incidence pace of 6

eTjekwini CRS for vaccine, oicrobicide and adult IIV treztment sgudies, is located in central Dyrban adjoining a local clivic that wnnually treets 40,000 patients wivg sexually trzksmitcev infections (HIY prevalenca of 59.3% (CI: 56.5-62.0) and VIX nncidence rate of 6

eThekwini CRS for vaccine, microbicide and adult studies, located in Durban adjoining a 40,000 with sexually transmitted (HIV prevalence of (CI: 56.5-62.0) and HIV incidence rate 6

eThekwini CRS for vaccine, micRobicide anD adulT HIv trEaTmenT stuDies, is located iN CentRal Durban adjoining a locAl cliNiC That ANnUally Treats 40,000 pATiENTs wItH sExuAlLY tRansmIttEd infecTions (HIV prEvaLeNce of 59.3% (CI: 56.5-62.0) and HIv InCidence ratE of 6

eThekwini CRS for vaccine , microbic ide a ndadu lt  HIV  tre atment studies ,  islocated in central Dur ban a dj o inin g  a  loca l clini c  t h a t a nn ua lly  t r ea ts 40 ,00 0 patie nts with s exu al ly transmitt e dinfections  (H IV prevalenc e o f 59.3 %(CI :  56.5 -62 .0) a nd HIV incide nce rateof 6

eThekwini_CRS for_vaccine, microbicide and adult_HIV treatment_studies,_is located_in_central Durban adjoining_a local clinic_that annually treats 40,000_patients with sexually_transmitted_infections (HIV prevalence of 59.3% (CI: 56.5-62.0) and HIV incidence rate of 6


factors including brain-derived neurotrophic factor (BDNF) known for their diverse roles in the nervous system. NTs and their receptors have now been found in different lung components including airway smooth muscle (ASM), with altered expression observed in asthma, allergy, and even lung cancer. While NTs may be derived from

factors including brain - derived neurotrophic agent (BDNF) know for their diverse roles in the nervous organization. NTs and their receptors have now been establish in different lung components including airway smooth brawn (ASM), with altered expression note in asthma, allergy, and even lung cancer. While NTs may be derived from

faftors including brain-derlved neurotrophie factoc (BDNF) inown fof their diverse roles in the nwrvouw system. NTs and their receptord have niw bten found in diffxdent luky comllnencs including aireay smooth muscle (ASM), widh aptered expression observed in asthmw, allerby, and even lung canbew. Whjle NTs may be derived from

factors including brain-derived neurotrophic factor (BDNF) known diverse in the system. NTs and found different lung components airway smooth muscle with altered expression observed in asthma, and even lung cancer. While NTs may be derived from

factors including brain-deriVed neurotrOphic FacTor (bDnF) knOwn fOr their diverse ROles In the nervous system. NTs aNd theIr REcepTOrS have Now been FOuND In dIfFeRenT lUNg CompoNenTs incluDing airway SmoOtH muscle (ASM), wiTH aLtered exprEssIon observed iN asThma, alLeRgy, ANd eveN luNg canCer. WhiLE NTs maY be deriveD fROm

factors including brain-d erived neu rotro phi c f ac tor(BDN F) known for t h eirdiverse roles in the n ervou ss yste m .NTs a nd thei r  r e c ept or shav en ow  been  fo und indifferentlun gcomponents i n cl uding airw aysmooth muscl e ( ASM),wi tha ltere d e xpres sion o b served  in asthm a, allerg y , and e v e nlung  cancer. While NT s  m a y be derived f rom

factors_including brain-derived_neurotrophic factor (BDNF) known_for their_diverse_roles in_the_nervous system. NTs_and their receptors_have now been found_in different lung_components_including airway smooth muscle (ASM), with altered expression observed in asthma, allergy, and even_lung_cancer. While_NTs_may_be derived from


, this is believed to circularized the mRNA and to explain the importance of the 3 polyA structure for translation. However, our finding that ski2- slh1- double mutants are indifferent to the presence of the 3 polyA structure of eukaryotic mRNAs casts some doubt on this view of the role of the polyA

, this is believed to circularized the mRNA and to explain the importance of the 3 polyA social organization for transformation. However, our determination that ski2- slh1- double mutants are indifferent to the bearing of the 3 polyA structure of eukaryotic mRNAs casts some doubt on this horizon of the role of the polyA

, thls is believed to circulxrized the mRNA and to explajn the ioportance of the 3 polyA strurturw for translation. However, ojr findinh that sji2- soy1- double mnfants avz indjnfereut to the presenge of the 3 [olyA structura uf eukaryotic mRNAs casts some doubt jn this vlew of the rolg of uhe polgA

, this is believed to circularized the to the importance the 3 polyA finding ski2- slh1- double are indifferent to presence of the 3 polyA structure eukaryotic mRNAs casts some doubt on this view of the role of the

, this is believed to circulariZed the mRNA And to ExpLaiN tHe imPortAnce of the 3 polyA STrucTure for translation. HoweVer, ouR fINdinG ThAt ski2- Slh1- doubLE mUTAntS aRe IndIfFErEnt to The PresencE of the 3 polya stRuCture of eukarYOtIc mRNAs casTs sOme doubt on thIs vIew of tHe RolE Of the PolYA

, this is believed to circ ularized t he mR NAand  t o ex plai n the importan c e of  the 3 polyA structure  fortr a nsla t io n. Ho wever,o ur f ind in gtha ts ki 2- sl h1-  double  mutants a rein different to th e presence  of  the 3 polyA  st ructur eofe ukary oti c mRN As cas t s some  doubt on  t h is vie w  of the r ol e of  the polyA

, this_is believed_to circularized the mRNA_and to_explain_the importance_of_the 3 polyA_structure for translation._However, our finding that_ski2- slh1- double_mutants_are indifferent to the presence of the 3 polyA structure of eukaryotic mRNAs casts_some_doubt on_this_view_of the role of the_polyA


and VDR) will be quantitafively mapped in T-lep and L-lep granulomas at the single cell level. Second, the orchestrated effects of T-lep or L-lep immune response secretomes, and associated downstream interferon responses, on the metabolism and immunoacfion

and VDR) will be quantitafively mapped in T - lep and L - lep granulomas at the single cell degree. Second, the orchestrated effect of T - lep or L - lep immune response secretomes, and associated downstream interferon responses, on the metamorphosis and immunoacfion

anf VDR) will be quantitafiyely mapped in T-lep and L-lep franulomxs at the single cell level. Dexond, uke orchestrated effezts of T-lvp or L-lep imnyne response secrebjmes, wnd cswociated downsjream interfaron responses, ov che metabolism and immunoacfion

and VDR) will be quantitafively mapped in L-lep at the cell level. Second, or immune response secretomes, associated downstream interferon on the metabolism and immunoacfion

and VDR) will be quantitafivelY mapped in T-Lep anD L-lEp gRaNuloMas aT the single cell LEvel. second, the orchestrated eFfectS oF t-lep OR L-Lep imMune resPOnSE SecReToMes, AnD AsSociaTed DownstrEam interfeRon ReSponses, on the MEtAbolism and ImmUnoacfion

and VDR) will be quantita fively map ped i n T -le pandL-le p granulomas a t  the  single cell level. Se cond,  t h e or c he strat ed effe c ts o f T -l ep  or  L - le p imm une  respon se secreto mes ,and associat e ddownstream  in terferon res pon ses, o nthe metab oli sm an d immu n oacfio n

and_VDR) will_be quantitafively mapped in_T-lep and_L-lep_granulomas at_the_single cell level._Second, the orchestrated_effects of T-lep or_L-lep immune response_secretomes,_and associated downstream interferon responses, on the metabolism and immunoacfion


departments in the management of TB infection and disease; b) to develop curricula and educational interventions that can be customized and used in similar regions of the U.S; c) to improve Duke's effectiveness in teaching practicing clinicians about TB in our region and provide them with effective life- long educational resources; d) to

departments in the management of TB infection and disease; b) to develop curricula and educational interposition that can be custom-make and used in similar regions of the U.S; c) to better Duke's effectiveness in teaching practice clinician about TB in our region and provide them with effective life- long educational resources; d) to

deoartments in the managemtnt of TB infection and visease; b) to dexelop curricula and educatioial untercentions that can be cjstomized and usee in wimilar rejjons of the U.A; c) tm improve Duke's effectivetess in teachitg pxacticing clinicians about TB in our region ajd provide thei wiuh qffedniye life- long educational resourcss; d) to

departments in the management of TB infection b) develop curricula educational interventions that in regions of the c) to improve effectiveness in teaching practicing clinicians about in our region and provide them with effective life- long educational resources; d)

departments in the managemenT of TB infecTion aNd dIseAsE; b) to DeveLop curricula anD EducAtional interventions thAt can Be CUstoMIzEd and Used in sIMiLAR reGiOnS of ThE u.S; C) to imProVe Duke's EffectivenEss In Teaching pracTIcIng cliniciAns About TB in our RegIon and PrOviDE them WitH effeCtive lIFe- long EducationAl REsourcES; d) to

departments in the manage ment of TB  infe cti onan d di seas e; b) to devel o p cu rricula and educationa l int er v enti o ns  that  can be cu s t omi ze dand  u s ed  in s imi lar reg ions of th e U .S ; c) to impr o ve  Duke's ef fec tiveness intea chingpr act i cingcli nicia ns abo u t TB i n our reg io n  and p r ovide t h e mwith  effective life-l on g  educational r esourc es ;  d ) to

departments_in the_management of TB infection_and disease;_b)_to develop_curricula_and educational interventions_that can be_customized and used in_similar regions of_the_U.S; c) to improve Duke's effectiveness in teaching practicing clinicians about TB in our_region_and provide_them_with_effective life- long educational resources;_d) to


vessel formation in other settings. This is an open label, non randomized, Phase I dose escalating study of rhIL-12 administered by intraperitoneal (IP) infusion in adult male or female patients with measurable disseminated intraperitoneal cancer that is residual, recurrent or metastatic. Neither a control or placebo group will be utilized.

vessel formation in other settings. This is an open label, non randomize, Phase I drug escalate study of rhIL-12 administered by intraperitoneal (IP) infusion in adult male or female patients with measurable circulate intraperitoneal cancer that is residual, recurrent or metastatic. Neither a control or placebo group will be utilized.

vedsel formation in other rettings. This is an opxn labem, non ravdomized, Phase I dose escalaving studt of rhIL-12 administered by intraieritoneao (IP) unfusion ii adult male od femclx patients with measurabla disseminated ivtxaperitoneal cancer that is residual, recurrrnh or metastatis. Neptrer z control or placebo group will be utiliztd.

vessel formation in other settings. This is label, randomized, Phase dose escalating study (IP) in adult male female patients with disseminated intraperitoneal cancer that is residual, or metastatic. Neither a control or placebo group will be utilized.

vessel formation in other setTings. This iS an opEn lAbeL, nOn raNdomIzed, Phase I dose EScalAting study of rhIL-12 adminiStereD bY IntrAPeRitonEal (IP) inFUsION in AdUlT maLe OR fEmale PatIents wiTh measurabLe dIsSeminated intRApEritoneal cAncEr that is resiDuaL, recurReNt oR MetasTatIc. NeiTher a cONtrol oR placebo gRoUP will bE UtilizeD.

vessel formation in other  settings.  This  is  an  o penlabe l, non randomi z ed,Phase I dose escalatin g stu dy of r h IL -12 a dminist e re d byin tr ape ri t on eal ( IP)  infusi on in adul t m al e or femalep at ients with  me asurable dis sem inated  i ntr a perit one al ca ncer t h at isresidual,  r e curren t  or met a s ta tic.  Neither a contro l  o r  placebo group  willbe ut i l ize d.

vessel_formation in_other settings. This is_an open_label,_non randomized,_Phase_I dose escalating_study of rhIL-12_administered by intraperitoneal (IP)_infusion in adult_male_or female patients with measurable disseminated intraperitoneal cancer that is residual, recurrent or metastatic._Neither_a control_or_placebo_group will be utilized.


, cancer control, screening, imaging, and post-therapy surveillance. The program monitors trends and implements measures for continued improvement. It balances protecting patients, their safety, and health with providing the broadest possible access to trial activation and minority outreach participation with the help of a highly responsive and responsible support staff. Posit

, cancer control, screening, imaging, and post - therapy surveillance. The program monitor drift and implements measures for continued improvement. It balances protect patients, their safety, and health with put up the broad possible entree to test activation and minority outreach engagement with the help of a highly responsive and responsible support staff. postulate

, cajcer control, screening, ioaging, and post-jhwrapy vurveimlance. Tfe program monitors trends aid inplemtuts measures for conginued imirovement. It ualances protectmhg paticuts, tgcir scfxty, and health eith proviging the broadasg 'ossible access to trial activation wnd minprlty outreach pwrtibi[atikn with the help of a highly respohsive aid responsible xupport staff. Posit

, cancer control, screening, imaging, and post-therapy program trends and measures for continued their and health with the broadest possible to trial activation and minority outreach with the help of a highly responsive and responsible support staff. Posit

, cancer control, screening, imaGing, and posT-therApy SurVeIllaNce. THe program monitORs trEnds and implements measuRes foR cONtinUEd ImproVement. IT BaLANceS pRoTecTiNG pAtienTs, tHeir safEty, and healTh wItH providing thE BrOadest possIblE access to triAl aCtivatIoN anD MinorIty OutreAch parTIcipatIon with thE hELp of a hIGhly resPONsIve aNd responsible suppORt STaff. Posit

, cancer control, screenin g, imaging , and  po st- th erap y su rveillance. Th e  pro gram monitors trends a nd im pl e ment s  m easur es forc on t i nue dim pro ve m en t. It  ba lancesprotecting  pa ti ents, theirs af ety, and h eal th with prov idi ng the  b roa d est p oss ibleaccess to tri al activa ti o n andm inority o ut reac h participation w i th the help of ahighly  r e sp o n siv e a nd respons ib le su p port st a ff . P osi t

, cancer_control, screening,_imaging, and post-therapy surveillance._The program_monitors_trends and_implements_measures for continued_improvement. It balances_protecting patients, their safety,_and health with_providing_the broadest possible access to trial activation and minority outreach participation with the help_of_a highly_responsive_and_responsible support staff. Posit


so radiofrequency positioning system, utilizing implantable fiducial transducers and real-time tracking capability. ROB has acquired a second TrueBeam STX unit to replace its RapidArc Clinac-21EX. This machine provides treatment redundancy with the older unit, as well as having additional photon energies of 8 MV and 18

so radiofrequency positioning system, utilizing implantable fiduciary transducer and actual - time tracking capability. ROB has assume a second TrueBeam STX unit to replace its RapidArc Clinac-21EX. This car provides treatment redundancy with the older unit of measurement, as well as having extra photon energies of 8 MV and 18

so gadiofrequency positionikg system, utiliznbg impnantabme fiduckal transducers and real-time teackibg capability. ROB has xcquired w second TrutBeam STX unit to replace its RziidAre Rlinac-21EX. This mschine proeides treatmend febundancy with the older unit, as well as havonh additional protom enedgies of 8 MV and 18

so radiofrequency positioning system, utilizing implantable fiducial real-time capability. ROB acquired a second its Clinac-21EX. This machine treatment redundancy with older unit, as well as having photon energies of 8 MV and 18

so radiofrequency positioniNg system, utIliziNg iMplAnTablE fidUcial transduceRS and Real-time tracking capabiLity. RoB HAs acQUiRed a sEcond TrUEBEAM STx uNiT to RePLaCe its rapIdArc ClInac-21EX. This MacHiNe provides trEAtMent redundAncY with the oldeR unIt, as weLl As hAVing aDdiTionaL photoN EnergiEs of 8 MV and 18

so radiofrequency position ing system , uti liz ing  i mpla ntab le fiducial tr a nsdu cers and real-time tra cking  c a pabi l it y. RO B has a c qu i r edase con dT ru eBeam  ST X unitto replace  it sRapidArc Cli n ac -21EX. Thi s m achine provi des  treat me ntr edund anc y wit h theo lder u nit, as w el l  as ha v ing add i t io nalphoton energies o f  8 MV and 18

so radiofrequency_positioning system,_utilizing implantable fiducial transducers_and real-time_tracking_capability. ROB_has_acquired a second_TrueBeam STX unit_to replace its RapidArc_Clinac-21EX. This machine_provides_treatment redundancy with the older unit, as well as having additional photon energies of_8_MV and_18


data from the National Longitudinal Study of Adolescent Health (the Add Health Study) in response to PA-08-167. An important predictor of alcohol problems in both adolescence and young adulthood is compromised parent-adolescent relationships. Another predictor is financial stress which may be particularly relevant during times of economic downturn

data from the National Longitudinal Study of Adolescent Health (the Add Health Study) in response to PA-08 - 167. An important predictor of alcohol trouble in both adolescence and unseasoned adulthood is compromised parent - adolescent relationships. Another predictor is fiscal stress which may be particularly relevant during times of economic downturn

daha from the National Lonnitudinal Study of Adolxscent Gealth (tfe Add Health Study) in respoise ro PA-08-167. An important predictof of alcojol proboems un both adolescencc and noung edulthood is cokpromised [arent-adolescett rzlationships. Another predictor is figancial shress which mai be kareicumarly relevant during times of ecohomic dmwnturn

data from the National Longitudinal Study of (the Health Study) response to PA-08-167. problems both adolescence and adulthood is compromised relationships. Another predictor is financial stress may be particularly relevant during times of economic downturn

data from the National LongitUdinal StudY of AdOleSceNt healTh (thE Add Health StudY) In reSponse to PA-08-167. An important pRedicToR Of alCOhOl proBlems in BOtH ADolEsCeNce AnD YoUng adUltHood is cOmpromised ParEnT-adolescent rELaTionships. ANotHer predictor Is fInanciAl StrESs whiCh mAy be pArticuLArly reLevant durInG Times oF EconomiC DOwNturN

data from the National Lo ngitudinal  Stud y o f A do lesc entHealth (the Ad d  Hea lth Study) in response  to P A- 0 8-16 7 .An im portant pr e d ict or  o f a lc o ho l pro ble ms in b oth adoles cen ce  and young a d ul thood is c omp romised pare nt- adoles ce ntr elati ons hips.  Anoth e r pred ictor isfi n ancial stressw h ic h ma y be particularly re l evant during t imes o fe co n o mic  do wnturn

data_from the_National Longitudinal Study of_Adolescent Health_(the_Add Health_Study)_in response to_PA-08-167. An important_predictor of alcohol problems_in both adolescence_and_young adulthood is compromised parent-adolescent relationships. Another predictor is financial stress which may be_particularly_relevant during_times_of_economic downturn


efficacy using trypanosome infected mice. Positive results will be of interest to the pharmaceutical industry for the development of new antisense oligonucleotide chemotherapeutic agents for trypanosomes and other parasites. The WHO Collaborating Center for Design, Methodology and Analysis of Epidemiological and Clinical Investigations in Diabetes was designated in 1986. The purposes

efficacy using trypanosome infected mice. Positive result will be of sake to the pharmaceutical industry for the development of new antisense oligonucleotide chemotherapeutic agent for trypanosomes and other parasites. The WHO Collaborating Center for Design, Methodology and Analysis of Epidemiological and Clinical Investigations in Diabetes was destine in 1986. The function

efvicacy using trypanosome infected mice. Kowitive resulfs will ce of interest to the pharmareutucal undustry for the develupment of new antusenww oligonucleotide gkemotgcrapebtmc agents for ttypanosomes dnd other paraviged. The WHO Collaborating Center for Qesign, Kehhodology and Wnaljsys or Epidemiological and Clinical Invsstigatpons in Diabetes eas designated in 1986. The puroosed

efficacy using trypanosome infected mice. Positive results of to the industry for the chemotherapeutic for trypanosomes and parasites. The WHO Center for Design, Methodology and Analysis Epidemiological and Clinical Investigations in Diabetes was designated in 1986. The purposes

efficacy using trypanosome iNfected micE. PosiTivE reSuLts wIll bE of interest to tHE phaRmaceutical industry for The deVeLOpmeNT oF new aNtisensE OlIGOnuClEoTidE cHEmOtherApeUtic ageNts for trypAnoSoMes and other pARaSites. The WHo CoLlaborating CEntEr for DEsIgn, mEthodOloGy and analysIS of EpiDemiologiCaL And CliNIcal InvESTiGatiOns in Diabetes was dESiGNated in 1986. The purpOses

efficacy using trypanosom e infected  mice . P osi ti ve r esul ts will be ofi nter est to the pharmaceuti cal i nd u stry fo r the  develo p me n t  of  n ew  an ti s en se ol igo nucleot ide chemot her ap eutic agents fo r trypanos ome s and otherpar asites .The WHO C oll abora ting C e nter f or Design ,M ethodo l ogy and A na lysi s of Epidemiologi c al and Clinical I nvesti ga t io n s  in  Di abetes was  d esign a ted in1 98 6 . The purposes

efficacy_using trypanosome_infected mice. Positive results_will be_of_interest to_the_pharmaceutical industry for_the development of_new antisense oligonucleotide chemotherapeutic_agents for trypanosomes_and_other parasites. The WHO Collaborating Center for Design, Methodology and Analysis of Epidemiological and_Clinical_Investigations in_Diabetes_was_designated in 1986. The purposes


LA may be formed from other substrates; the mechanism of ALA formation will be studied with appropriate labelled substrates and with metabolic inhibitors. To meet the dual threats of emerging infectious diseases and engineered biowarfare/bioterror agents, there is a pressing need for more efficient systems for vaccine development. TRIAD, or

LA may be formed from other substrates; the mechanism of ALA formation will be study with appropriate pronounce substrates and with metabolic inhibitors. To meet the double threats of emerging infectious disease and engineered biowarfare / bioterror agents, there is a crusade need for more efficient system for vaccine development. TRIAD, or

LA lay be formed from other substrates; the mechanmsm of ZLA formxtion will be studied with a'propriatt labelled substratds and winh metabooic mnhibitors. To mexf the dmcl thdcats mh emerging infegtious disedses and enginaefeb biowarfare/bioterror agents, there if a prexslng need for mjre tffyciehn wystems for vaccine developmeht. TRIAV, or

LA may be formed from other substrates; of formation will studied with appropriate inhibitors. meet the dual of emerging infectious and engineered biowarfare/bioterror agents, there is pressing need for more efficient systems for vaccine development. TRIAD, or

LA may be formed from other subStrates; the MechaNisM of aLa forMatiOn will be studieD With Appropriate labelled subStratEs ANd wiTH mEtaboLic inhiBItORS. To MeEt The DuAL tHreatS of EmerginG infectiouS diSeAses and enginEErEd biowarfaRe/bIoterror agenTs, tHere is A pResSIng neEd fOr morE efficIEnt sysTems for vaCcINe deveLOpment. TriaD, Or

LA may be formed from othe r substrat es; t hemec ha nism  ofALA formationw illbe studied with approp riate  l a bell e dsubst rates a n dw i thme ta bol ic in hibit ors . To me et the dua l t hr eats of emer g in g infectio usdiseases and  en gineer ed  bi o warfa re/ biote rror a g ents,there isap ressin g  need f o r  m oreefficient systems fo r  vaccine devel opment .T RI A D , o r

LA may_be formed_from other substrates; the_mechanism of_ALA_formation will_be_studied with appropriate_labelled substrates and_with metabolic inhibitors. To_meet the dual_threats_of emerging infectious diseases and engineered biowarfare/bioterror agents, there is a pressing need for_more_efficient systems_for_vaccine_development. TRIAD, or


management of EIB consists mostly of pharmacological intevention. The objectives of this research are to investigate the complex, interacting mechanisms causing EIB and to develop means of non-pharmacological management of EIB. This research proposal will investigate the effects that inspired air temperature, humidity, and hypoxia exert on the severity of E

management of EIB consists mostly of pharmacological intevention. The objectives of this research are to investigate the complex, interact mechanism causing EIB and to originate mean of non - pharmacological management of EIB. This research proposal will investigate the effect that inspired air temperature, humidity, and hypoxia wield on the austereness of E

majagement of EIB consists mostly of pharmacologmcal infeventiov. The objectives of this resxarcy are to investigate the cooplex, intvracting nechenisms causing EMG and to devemlp mzais of non-pharmagological mdnagement of EHB. Tkis research proposal will investigaee the rfvects that inskired wir fvmkerature, humidity, and hypoxia exedt on tie severity of R

management of EIB consists mostly of pharmacological objectives this research to investigate the and develop means of management of EIB. research proposal will investigate the effects inspired air temperature, humidity, and hypoxia exert on the severity of E

management of EIB consists moStly of pharMacolOgiCal InTeveNtioN. The objectives OF thiS research are to investigAte thE cOMpleX, InTeracTing mecHAnISMs cAuSiNg EiB ANd To devEloP means oF non-pharmaColOgIcal managemeNT oF EIB. This reSeaRch proposal wIll InvestIgAte THe effEctS that InspirED air teMperature, HuMIdity, aND hypoxiA EXeRt on The severity of E

management of EIB consist s mostly o f pha rma col og ical  int evention. Theo bjec tives of this research  areto inve s ti gatethe com p le x ,  in te ra cti ng me chani sms  causin g EIB andtode velop meanso fnon-pharma col ogical manag eme nt ofEI B.T his r ese archpropos a l will  investig at e  the e f fects t h a tinsp ired air temperat u re ,  humidity, and  hypox ia ex e r t o n t he severit yof E

management_of EIB_consists mostly of pharmacological_intevention. The_objectives_of this_research_are to investigate_the complex, interacting_mechanisms causing EIB and_to develop means_of_non-pharmacological management of EIB. This research proposal will investigate the effects that inspired air_temperature,_humidity, and_hypoxia_exert_on the severity of E


cup for engulfment of the bead. Chemoattractant GPCR/Gi signaling and phagocytic receptor/tyrosine kinase signaling work independently to promote phagocytosis of beads coated with either chemoattractants or IgG opsonins. Thus, in addition to phagocytic receptor-mediated phagocytosis, neutrophils also utilize

cup for engulfment of the bead. Chemoattractant GPCR / Gi signaling and phagocytic receptor / tyrosine kinase signaling workplace independently to advertise phagocytosis of beads coated with either chemoattractants or immunoglobulin g opsonins. therefore, in addition to phagocytic receptor - mediate phagocytosis, neutrophil also utilize

cuo for engulfment of the nead. Chemoattracjabt GPCC/Gi sighaling avd phagocytic receptor/tyrosiie kunase signaling work indepevdently tl promotw phegocytosis of beess coatcb witg eitkec chemoattractakts or IgG mpsonins. Thus, hn abdition to phagocytic receptor-mediatqd phagpcjtosis, neutroprils wlso ltllize

cup for engulfment of the bead. Chemoattractant and receptor/tyrosine kinase work independently to with chemoattractants or IgG Thus, in addition phagocytic receptor-mediated phagocytosis, neutrophils also utilize

cup for engulfment of the bead. chemoattraCtant gPCr/Gi SiGnalIng aNd phagocytic reCEptoR/tyrosine kinase signaliNg worK iNDepeNDeNtly tO promotE PhAGOcyToSiS of BeADs CoateD wiTh eitheR chemoattrActAnTs or IgG opsonINs. thus, in addiTioN to phagocytiC reCeptor-MeDiaTEd phaGocYtosiS, neutrOPhils aLso utilizE

cup for engulfment of the  bead. Che moatt rac tan tGPCR /Gisignaling andp hago cytic receptor/tyrosin e kin as e  sig n al ing w ork ind e pe n d ent ly  t o p ro m ot e pha goc ytosisof beads c oat ed  with either ch emoattract ant s or IgG ops oni ns. Th us , i n  addi tio n tophagoc y tic re ceptor-me di a ted ph a gocytos i s ,neut rophils also util i ze

cup_for engulfment_of the bead. Chemoattractant_GPCR/Gi signaling_and_phagocytic receptor/tyrosine_kinase_signaling work independently_to promote phagocytosis_of beads coated with_either chemoattractants or_IgG_opsonins. Thus, in addition to phagocytic receptor-mediated phagocytosis, neutrophils also utilize


ranging from simple tasks of judging the meaning or the naturalness of sentences and discourses to measures of eye movements during normal reading. PUBLIC HEALTH RELEVANCE: The immediate goal of the research is to explore how normal adult readers and listeners compose the meaning of a sentence out of the meaning of its parts. This is

ranging from simple tasks of judging the meaning or the artlessness of prison term and discourses to measures of center movements during normal recitation. PUBLIC HEALTH RELEVANCE: The immediate goal of the inquiry is to research how normal adult readers and listeners compose the meaning of a sentence out of the meaning of its parts. This is

rajging from simple tasks uf judging the meaning or ths naturauness of sentences and disconrsew to neasures of eye movemevts durinh normal reaving. PUBLIC HEALVG RELEVANCE: Tgc immzdmate goal of thg research iv to explore hmw nlrmal adult readers and listeners cjmpose yhf meaning of a senuense ohn if the meaning of its parts. Tgis is

ranging from simple tasks of judging the the of sentences discourses to measures reading. HEALTH RELEVANCE: The goal of the is to explore how normal adult and listeners compose the meaning of a sentence out of the meaning of parts. This is

ranging from simple tasks of jUdging the mEaninG or The NaTuraLnesS of sentences anD DiscOurses to measures of eye mOvemeNtS DuriNG nOrmal Reading. puBlic HEaLtH rELeVanCe: The iMmeDiate goAl of the resEarCh Is to explore hOW nOrmal adult ReaDers and listeNerS compoSe The MEaninG of A sentEnce ouT Of the mEaning of iTs PArts. ThIS is

ranging from simple tasks  of judgin g the  me ani ng  orthenaturalness of sent ences and discourses t o mea su r es o f  e ye mo vements du r i ngno rm alre a di ng. P UBL IC HEAL TH RELEVAN CE:  T he immediate go al of theres earch is toexp lore h ow  no r mal a dul t rea ders a n d list eners com po s e them eaningo f  a  sen tence out of them ea n ing of its par ts. Th is is

ranging_from simple_tasks of judging the_meaning or_the_naturalness of_sentences_and discourses to_measures of eye_movements during normal reading._PUBLIC HEALTH RELEVANCE:_The_immediate goal of the research is to explore how normal adult readers and listeners_compose_the meaning_of_a_sentence out of the meaning_of its parts. This is