, imaging, and technologies used in interventional/procedural medical care make the field of anesthesiology/perioperative medicine rich with research career development opportunities. The goal of this program is to address the as yet unmet need to train more committed physician-scientist anesthesiologists. PPRT

, imaging, and technologies used in interventional / procedural aesculapian caution make the field of anesthesiology / perioperative music rich with inquiry career development opportunities. The finish of this program is to address the as yet unmet motivation to train more committed physician - scientist anesthesiologist. PPRT

, imwging, and technologies ured in intervenjiinal/prmcedurzl medicxl care make the field of anxsthwsioligy/perioperative medickne rich aith reswarci career development opportunifles. Tke goal of this krogram is tm address the ds yzt unmet need to train more committeq physiviwn-scientist angsthexyolofpsus. PPRT

, imaging, and technologies used in interventional/procedural make field of medicine rich with goal this program is address the as unmet need to train more committed anesthesiologists. PPRT

, imaging, and technologies useD in interveNtionAl/pRocEdUral MediCal care make the FIeld Of anesthesiology/periopEratiVe MEdicINe Rich wIth reseARcH CAreEr DeVelOpMEnT oppoRtuNities. THe goal of thIs pRoGram is to addrESs The as yet unMet Need to train mOre CommitTeD phYSiciaN-scIentiSt anesTHesiolOgists. PPRt

, imaging, and technologie s used ininter ven tio na l/pr oced ural medical c a re m ake the field of anest hesio lo g y/pe r io perat ive med i ci n e  ri ch  w ith  r e se archcar eer dev elopment o ppo rt unities. The go al of this  pr ogram is toadd ress t he  as yet u nme t nee d to t r ain mo re commit te d  physi c ian-sci e n ti st a nesthesiologists. PP R T

, imaging,_and technologies_used in interventional/procedural medical_care make_the_field of_anesthesiology/perioperative_medicine rich with_research career development_opportunities. The goal of_this program is_to_address the as yet unmet need to train more committed physician-scientist anesthesiologists. PPRT


research will begin to provide necessary data and analysis of addict income, expenditures, crime, and drug use behavior. This grant is a pilot study investigating the economic behavior of street-level opiate addicts in the East Harlem area of New York City. The study will obtain data about the total income and expenditures of addict

research will begin to provide necessary data and psychoanalysis of addict income, consumption, crime, and drug use behavior. This concession is a pilot sketch investigating the economic demeanor of street - level opiate addicts in the East Harlem area of New York City. The survey will obtain data about the full income and expenditures of addict

redearch will begin to proyide necessary dcra and analyais of aadict income, expenditures, crmme, qnd deug use behavior. This erant is w pilot wtudb investigating vge economic bsmaviox if street-level opiate adgicts in the Edsg Karlem area of New York City. The stuqy will ohtain data aboot tht tjtal pngome and expenditures of addict

research will begin to provide necessary data of income, expenditures, and drug use pilot investigating the economic of street-level opiate in the East Harlem area of York City. The study will obtain data about the total income and expenditures addict

research will begin to providE necessary Data aNd aNalYsIs of AddiCt income, expendITureS, crime, and drug use behaviOr. ThiS gRAnt iS A pIlot sTudy invEStIGAtiNg ThE ecOnOMiC behaVioR of streEt-level opiAte AdDicts in the EaST HArlem area oF NeW York City. The StuDy will ObTaiN Data aBouT the tOtal inCOme and ExpendituReS Of addiCT

research will begin to pr ovide nece ssary  da taan d an alys is of addict i n come , expenditures, crime,  anddr u g us e  b ehavi or. Thi s  g r a ntis  a  pi lo t  s tudyinv estigat ing the ec ono mi c behavior o f  s treet-leve l o piate addict s i n theEa stH arlem  ar ea of  New Y o rk Cit y. The st ud y  willo btain d a t aabou t the total incom e  a n d expenditures  of ad di c t

research_will begin_to provide necessary data_and analysis_of_addict income,_expenditures,_crime, and drug_use behavior. This_grant is a pilot_study investigating the_economic_behavior of street-level opiate addicts in the East Harlem area of New York City._The_study will_obtain_data_about the total income and_expenditures of addict


learning and producing song. Area X is a part of the neural circuitry involved in song acquisition and modulation. Area X is also a part of the avian basal ganglia and receives a strong input from midbrain dopaminergic nuclei. Cells in Area X respond to changes in social context: their activity changes depending on whether males are singing in

learning and producing song. Area X is a part of the nervous circuitry imply in song acquisition and modulation. Area X is besides a part of the avian basal ganglion and receives a strong input signal from midbrain dopaminergic nuclei. Cells in Area X react to changes in social context: their activity change depending on whether males are singing in

lewrning and producing sonn. Area X is a patt of thx neuram circuigry involved in song acquisivion and nodulation. Area X is auso a parn of the qviai basal ganglia ehd recelres a dtroug input from micbrain dopdminergic nuclai. Czlls in Area X respond to changes in social clntext: their astivptr chznges depending on whether males ade singpng in

learning and producing song. Area X is of neural circuitry in song acquisition also part of the basal ganglia and a strong input from midbrain dopaminergic Cells in Area X respond to changes in social context: their activity changes on whether males are singing in

learning and producing song. ARea X is a parT of thE neUraL cIrcuItry Involved in song ACquiSition and modulation. AreA X is aLsO A parT Of The avIan basaL GaNGLia AnD rEceIvES a StronG inPut from Midbrain doPamInErgic nuclei. CELlS in Area X reSpoNd to changes iN soCial coNtExt: THeir aCtiVity cHanges DEpendiNg on whethEr MAles arE Singing IN

learning and producing so ng. Area X  is a  pa rtof  the  neu ral circuitryi nvol ved in song acquisitio n and  m o dula t io n. Ar ea X is al s o  apa rt  of  t h eavian  ba sal gan glia and r ece iv es a strongi np ut from mi dbr ain dopamine rgi c nucl ei . C e lls i n A rea X  respo n d to c hanges in  s o cial c o ntext:t h ei r ac tivity changes de p en d ing on whether  males  a r es i ngi ngin

learning_and producing_song. Area X is_a part_of_the neural_circuitry_involved in song_acquisition and modulation._Area X is also_a part of_the_avian basal ganglia and receives a strong input from midbrain dopaminergic nuclei. Cells in_Area_X respond_to_changes_in social context: their activity_changes depending on whether males_are singing_in


The long-term goal of our project is to gain a greater understanding of the intracellular signaling pathways that provide neuroprotection to both rod and cone photoreceptor cells. The specific objective is to investigate the role of IR and IGF-1R in the regulation of photoreceptor structure and function. To this end, we will determine

The long - term goal of our project is to profit a great understanding of the intracellular signaling pathways that put up neuroprotection to both rod and cone photoreceptor cells. The specific aim is to investigate the role of IR and IGF-1R in the regulation of photoreceptor structure and affair. To this end, we will determine

Thf long-term goal of our pvoject is to gaiu a greeter unserstandkng of the intracellular sigialibg paukways that provide nduroprotebtion to voth eod and cois photovzceptkv celnw. The specific objective is to investicage the role of IR and IGF-1R in the regtlation ov photoreceptot strlceure and function. To this end, we will setermiie

The long-term goal of our project is a understanding of intracellular signaling pathways rod cone photoreceptor cells. specific objective is investigate the role of IR and in the regulation of photoreceptor structure and function. To this end, we will

The long-term goal of our projeCt is to gain A greaTer UndErStanDing Of the intracellULar sIgnaling pathways that prOvide NeURoprOTeCtion To both rOD aND ConE pHoTorEcEPtOr celLs. THe speciFic objectiVe iS tO investigate THe Role of IR anD IGf-1R in the regulAtiOn of phOtOreCEptor StrUcturE and fuNCtion. TO this end, wE wILl deteRMine

The long-term goal of our  project i s togai n a  g reat er u nderstanding o f  the  intracellular signali ng pa th w ayst ha t pro vide ne u ro p r ote ct io n t ob ot h rod  an d conephotorecep tor  c ells. The sp e ci fic object ive  is to inves tig ate th erol e  of I R a nd IG F-1R i n  the r egulation  o f  photo r eceptor s tr uctu re and function.T ot his end, we wi ll det er m in e

The_long-term goal_of our project is_to gain_a_greater understanding_of_the intracellular signaling_pathways that provide_neuroprotection to both rod_and cone photoreceptor_cells._The specific objective is to investigate the role of IR and IGF-1R in the_regulation_of photoreceptor_structure_and_function. To this end, we_will determine


emic progression but not long-term hematopoietic stem cell engraftment. In this revised competitive renewal application, we propose to examine the molecular pathways to understand the mechanism of CREB function in normal and aberrant hematopoiesis. We propose four specific aims. In Aim 1, we will study the role of CREB on

emic progression but not long - term hematopoietic stem cellular telephone engraftment. In this revise competitive renewal application, we nominate to probe the molecular pathways to understand the mechanism of CREB routine in normal and aberrant hematopoiesis. We aim four specific aims. In Aim 1, we will study the role of CREB on

emif progression but not lokg-term hematopoigtuc stek cell engraftoent. In this revised competivive reneqal application, we prooose to eqamine thw moowcular patisays to underaband chx mechanism of GREB functimn in normal atd ayerrant hematopoiesis. We propose fouw specigif aims. In Aim 1, we eyll anuby the role of CREB on

emic progression but not long-term hematopoietic stem In revised competitive application, we propose to the mechanism of function in normal aberrant hematopoiesis. We propose four specific In Aim 1, we will study the role of CREB on

emic progression but not long-Term hematoPoietIc sTem CeLl enGrafTment. In this revISed cOmpetitive renewal appliCatioN, wE PropOSe To exaMine the MOlECUlaR pAtHwaYs TO uNdersTanD the mecHanism of CReB fUnCtion in normaL AnD aberrant hEmaTopoiesis. We pRopOse fouR sPecIFic aiMs. IN Aim 1, wE will sTUdy the Role of CREb oN

emic progression but not l ong-term h emato poi eti cstem  cel l engraftment. In t his revised competitiv e ren ew a l ap p li catio n, we p r op o s e t oex ami ne th e mol ecu lar pat hways to u nde rs tand the mec h an ism of CRE B f unction in n orm al and  a ber r ant h ema topoi esis.W e prop ose foursp e cifica ims. In A im  1,we will study the ro l e of CREB on

emic progression_but not_long-term hematopoietic stem cell_engraftment. In_this_revised competitive_renewal_application, we propose_to examine the_molecular pathways to understand_the mechanism of_CREB_function in normal and aberrant hematopoiesis. We propose four specific aims. In Aim 1,_we_will study_the_role_of CREB on


the primary responsibility for the child's care. Traditional hospital-based interventions have focused on the peritransplant period, but given the prolonged and demanding period of recovery (6-12 months), alternative interventions are needed. To address these issues, we are collaborating to develop a highly transportable transplant-specific module,

the primary responsibility for the child's care. Traditional hospital - based interposition have concenter on the peritransplant period, but given the prolonged and demanding menstruation of recovery (6 - 12 months), alternate interventions are needed. To address these emergence, we are collaborating to develop a highly movable transplant - specific module,

thf primary responsibility for the child's care. Tcaditiohal hospktal-based interventions have ficusee on the peritransplang period, hut giveb tht prolonged and dxjanding perios of xerovery (6-12 months), slternativa interventionv xrz needed. To address these issues, we wre colkahorating to dedelok a higgly transportable transplant-specifjc modune,

the primary responsibility for the child's care. interventions focused on peritransplant period, but period recovery (6-12 months), interventions are needed. address these issues, we are collaborating develop a highly transportable transplant-specific module,

the primary responsibility fOr the child'S care. traDitIoNal hOspiTal-based intervENtioNs have focused on the periTransPlANt peRIoD, but gIven the PRoLONgeD aNd DemAnDInG periOd oF recoveRy (6-12 months), alTerNaTive intervenTIoNs are needeD. To Address these IssUes, we aRe ColLAboraTinG to deVelop a HIghly tRansportaBlE TranspLAnt-specIFIc ModuLe,

the primary responsibilit y for thechild 'scar e.  Tra diti onal hospital- b ased  interventions have fo cused  o n  the pe ritra nsplant pe r i od,  b ut  gi ve n  t he pr olo nged an d demandin g p er iod of recov e ry  (6-12 mon ths ), alternati veinterv en tio n s are  ne eded.  To ad d ress t hese issu es ,  we ar e  collab o r at ingto develop a high l yt ransportable t ranspl an t -s p e cif icmodule,

the_primary responsibility_for the child's care._Traditional hospital-based_interventions_have focused_on_the peritransplant period,_but given the_prolonged and demanding period_of recovery (6-12_months),_alternative interventions are needed. To address these issues, we are collaborating to develop a_highly_transportable transplant-specific_module,


. Deficient expression of APE results in a heightened sensitivity to radiation and alkylating agents with resultant tissue damage. Logically it follows that APE may also play a role in the sensitivity to malignant cells to DNA damaging therapeutic agents. Many cancer therapeutic agents will induce apoptosis or programmed cell death, however little is known about

. Deficient expression of APE results in a heightened sensitivity to radiation sickness and alkylating agent with resultant tissue damage. Logically it follows that APE may besides play a role in the sensitivity to malignant cells to deoxyribonucleic acid damaging curative agents. Many cancer therapeutic agents will induce apoptosis or programmed cell end, however little is known about

. Devicient expression of APT results in a henthtenev sensifivity tu radiation and alkylating ajentw wity resultant tissue damxge. Logicwlly it dolliqs that APX may also plag a rmoe in the sensltivity to kalignant cellv go DNA damaging therapeutic agents. Magy cancrr therapeutic adentx wilm induce apoptosis or programmed csll deauh, however little os known about

. Deficient expression of APE results in sensitivity radiation and agents with resultant that may also play role in the to malignant cells to DNA damaging agents. Many cancer therapeutic agents will induce apoptosis or programmed cell death, however is known about

. Deficient expression of APE rEsults in a hEightEneD seNsItivIty tO radiation and aLKylaTing agents with resultanT tissUe DAmagE. loGicalLy it folLOwS THat aPe mAy aLsO PlAy a roLe iN the senSitivity to MalIgNant cells to Dna dAmaging theRapEutic agents. MAny Cancer ThEraPEutic AgeNts wiLl induCE apoptOsis or proGrAMmed ceLL death, hOWEvEr liTtle is known about

. Deficient expression ofAPE result s ina h eig ht ened  sen sitivity to ra d iati on and alkylating agen ts wi th resu l ta nt ti ssue da m ag e .  Lo gi ca lly  i t  f ollow s t hat APE  may alsopla ya role in th e  s ensitivity  to  malignant c ell s to D NA  da m aging  th erape utic a g ents.Many canc er therap e utic ag e n ts  wil l induce apoptosi s  o r  programmed ce ll dea th ,  h o w eve r l ittle is k no wn ab o ut

. Deficient_expression of_APE results in a_heightened sensitivity_to_radiation and_alkylating_agents with resultant_tissue damage. Logically_it follows that APE_may also play_a_role in the sensitivity to malignant cells to DNA damaging therapeutic agents. Many cancer_therapeutic_agents will_induce_apoptosis_or programmed cell death, however_little is known about


be small under steady state conditions. Therefore, we will perturb the system by inducing oxidation of membranes in vitro (using primarily endogenous oxidants) in order to amplify existing differences. Our preliminary studies show that mitochondrial cholesterol is more oxidizable in PD than in controls. Mitochondrial and synaptosomal fractions will be oxidized by

be small under steady state conditions. consequently, we will perturb the arrangement by inducing oxidation of membranes in vitro (use chiefly endogenous oxidants) in order to amplify exist differences. Our preliminary studies prove that mitochondrial cholesterol is more oxidizable in PD than in controls. Mitochondrial and synaptosomal fraction will be oxidized by

be small under steady statt conditions. Thergfire, we will lerturb ghe system by inducing oxidavion of mtibranes in vitro (uring primwrily eneogeious oxidants) in order to ampljny exnsving differencex. Our prelhminary studiev rhlw that mitochondrial cholesterol if more pxldizable in PD tham in dontrols. Mitochondrial and synaptoaomal fgactions will be pxidized by

be small under steady state conditions. Therefore, perturb system by oxidation of membranes oxidants) order to amplify differences. Our preliminary show that mitochondrial cholesterol is more in PD than in controls. Mitochondrial and synaptosomal fractions will be oxidized by

be small under steady state coNditions. ThErefoRe, wE wiLl PertUrb tHe system by induCIng oXidation of membranes in vItro (uSiNG priMArIly enDogenouS OxIDAntS) iN oRdeR tO AmPlify ExiSting diFferences. OUr pReLiminary studIEs Show that miTocHondrial cholEstErol is MoRe oXIdizaBle In PD tHan in cONtrols. mitochondRiAL and syNAptosomAL FrActiOns will be oxidized BY

be small under steady sta te conditi ons.The ref or e, w e wi ll perturb the syst em by inducing oxidati on of  m e mbra n es  in v itro (u s in g pri ma ri lyen d og enous  ox idants)  in ordertoam plify existi n gdifference s.Our prelimin ary  studi es  sh o w tha t m itoch ondria l  chole sterol is  m o re oxi d izablei n  P D th an in controls. M i to c hondrial and s ynapto so m al f rac tio ns will be  o xidiz e d by

be_small under_steady state conditions. Therefore,_we will_perturb_the system_by_inducing oxidation of_membranes in vitro_(using primarily endogenous oxidants)_in order to_amplify_existing differences. Our preliminary studies show that mitochondrial cholesterol is more oxidizable in PD_than_in controls._Mitochondrial_and_synaptosomal fractions will be oxidized_by


and unmethylated DNA ligands? to create detailed maps of the effect of methylation on binding affinity for a broad panel of human transcription factors from various structural families. Second, we will perform detailed computational analyses and follow-up experiments to test the hypothesis that methylation causes local changes in DNA shape, which in turn modify TF binding

and unmethylated DNA ligands? to create detailed maps of the effect of methylation on bind affinity for a wide panel of human transcription factors from respective structural families. Second, we will do detailed computational analyses and follow - up experiments to screen the hypothesis that methylation causes local change in DNA shape, which in turn modify TF binding

anf unmethylated DNA liganas? to create dejauled meps of fhe effezt of methylation on binding adfiniuj for a broad panel ow human tganscriptuon hactors from varmkus strmeturam famnlmes. Second, we wlll perform detailed compgtxtnonal analyses and follow-up experimegts to yedt the hypothefis uhae mefhylation causes local changes in SNA shake, which in turn mpdify TF binding

and unmethylated DNA ligands? to create detailed the of methylation binding affinity for transcription from various structural Second, we will detailed computational analyses and follow-up experiments test the hypothesis that methylation causes local changes in DNA shape, which in modify TF binding

and unmethylated DNA ligands? To create deTaileD maPs oF tHe efFect Of methylation oN BindIng affinity for a broad paNel of HuMAn trANsCriptIon factORs FROm vArIoUs sTrUCtUral fAmiLies. SecOnd, we will pErfOrM detailed comPUtAtional anaLysEs and follow-uP exPerimeNtS to TEst thE hyPotheSis thaT MethylAtion causEs LOcal chANges in Dna ShApe, wHich in turn modify Tf BiNDing

and unmethylated DNA liga nds? to cr eatedet ail ed  map s of  the effect of meth ylation on binding aff inity  f o r ab ro ad pa nel ofh um a n  tr an sc rip ti o nfacto rsfrom va rious stru ctu ra l families.S ec ond, we wi llperform deta ile d comp ut ati o nal a nal ysesand fo l low-up  experime nt s  to te s t the h y p ot hesi s that methylatio n  c a uses local cha nges i nD NA s hap e,which in t ur n mod i fy TF b i nd i n g

and_unmethylated DNA_ligands? to create detailed_maps of_the_effect of_methylation_on binding affinity_for a broad_panel of human transcription_factors from various_structural_families. Second, we will perform detailed computational analyses and follow-up experiments to test the_hypothesis_that methylation_causes_local_changes in DNA shape, which_in turn modify TF binding


% from the labelled potency. Predilution of both products in either albumin or factor VIII deficient plasma also affects the potency, with those samples prediluted in deficient plasma having overall higher values. It is planned to do in vivo half life and recovery studies. Control of the primary tumor is a major goal of radiotherapy for

% from the labelled potency. Predilution of both products in either albumin or factor VIII insufficient plasma besides affect the potency, with those samples prediluted in insufficient plasma having overall higher values. It is plan to do in vivo half life and convalescence study. command of the primary tumor is a major goal of radiotherapy for

% frlm the labelled potency. Kredilution of bojh produrts in sither aubumin or factor VIII deficixnt plasmq also affects the potdncy, with those sqmplts prediluted in vsficienb plaala hcvmng overall higmer values. Ht is planned do dl in vivo half life and recovery sttdies. Cpnhrol of the prymarj eumod is a major goal of radiotherapy ror

% from the labelled potency. Predilution of in albumin or VIII deficient plasma those prediluted in deficient having overall higher It is planned to do in half life and recovery studies. Control of the primary tumor is a major of radiotherapy for

% from the labelled potency. PreDilution of Both pRodUctS iN eitHer aLbumin or factor viII dEficient plasma also affeCts thE pOTencY, WiTh thoSe samplES pREDilUtEd In dEfICiEnt plAsmA having Overall higHer VaLues. It is planNEd To do in vivo HalF life and recoVerY studiEs. conTRol of The PrimaRy tumoR Is a majOr goal of rAdIOtheraPY for

% from the labelled potenc y. Predilu tionofbot hprod ucts  in either alb u minor factor VIII deficie nt pl as m a al s oaffec ts thep ot e n cy,  w it h t ho s esampl espredilu ted in def ici en t plasma hav i ng  overall h igh er values. I t i s plan ne d t o  do i n v ivo h alf li f e andrecoveryst u dies.C ontrolo f  t he p rimary tumor is a ma j or goal of rad iother ap y  f o r

% from_the labelled_potency. Predilution of both_products in_either_albumin or_factor_VIII deficient plasma_also affects the_potency, with those samples_prediluted in deficient_plasma_having overall higher values. It is planned to do in vivo half life and_recovery_studies. Control_of_the_primary tumor is a major_goal of radiotherapy for


and its effects are altered by mutations that cause human disease. Cx26 and Cx32 are widely distributed in the body. Mutations of Cx26 are responsible for over half the inherited sensorineural deafness worldwide, and also cause serious disfiguring skin disorders. Mutations of Cx32 cause a peripheral demyelination

and its effects are altered by mutations that cause human disease. Cx26 and Cx32 are wide distribute in the body. mutation of Cx26 are creditworthy for over half the inherited sensorineural deafness worldwide, and also cause dangerous disfiguring skin disorders. mutant of Cx32 cause a peripheral demyelination

anf its effects are alterea by mutations jhqt cauve humzn diseare. Cx26 and Cx32 are widely distcibured ib the body. Mutations ow Cx26 are gesponsiboe fie over halh the inmzrites senvirineural deafkess worldwhde, and also cdure serious disfiguring skin disorders. Mutatipnd of Cx32 cause w pegi[herzl demyelination

and its effects are altered by mutations human Cx26 and are widely distributed Cx26 responsible for over the inherited sensorineural worldwide, and also cause serious disfiguring disorders. Mutations of Cx32 cause a peripheral demyelination

and its effects are altered by Mutations tHat caUse HumAn DiseAse. CX26 and Cx32 are widelY DistRibuted in the body. MutatiOns of cx26 ARe reSPoNsiblE for oveR HaLF The InHeRitEd SEnSorinEurAl deafnEss worldwiDe, aNd Also cause serIOuS disfiguriNg sKin disorders. mutAtions Of cx32 cAUse a pEriPheraL demyeLInatioN

and its effects are alter ed by muta tions  th atca usehuma n disease. Cx2 6  and  Cx32 are widely distr ibute di n th e  b ody.Mutatio n so f  Cx 26  a rere s po nsibl e f or over  half theinh er ited sensori n eu ral deafne ssworldwide, a ndalso c au ses eriou s d isfig urings kin di sorders.Mu t ations of Cx32 c au se a  peripheral demye l in a tion

and_its effects_are altered by mutations_that cause_human_disease. Cx26_and_Cx32 are widely_distributed in the_body. Mutations of Cx26_are responsible for_over_half the inherited sensorineural deafness worldwide, and also cause serious disfiguring skin disorders. Mutations_of_Cx32 cause_a_peripheral_demyelination


studies. Issues regarding the opimal protocol and set of sequence parameters needed for various diffusion studies are under investigation, which includes more rapid protocols for the assessment of stroke and more high resolution protocols for diffusion tensor imaging of white matter tracts. The effort is being translated from 3T to newer field field studies at 7T. OB

studies. Issues regarding the opimal protocol and set of succession argument needed for various dissemination study are under investigation, which includes more rapid protocol for the assessment of stroke and more eminent resolution protocols for dispersion tensor imaging of white topic tracts. The effort is being translated from 3 T to newer field field study at 7T. OB

stkdies. Issues regarding tme opimal protocol and vet of sequencd parameters needed for variluw difdusion studies are unddr investpgation, wyich uncludes more rapid protkgols yoc the assessmenj of stroke dnd more high seropution protocols for diffusion tensjr imagonh of white matjer tgasts. Fhe effort is being translated froj 3T to iewer field fiekd studies at 7T. OB

studies. Issues regarding the opimal protocol and sequence needed for diffusion studies are rapid for the assessment stroke and more resolution protocols for diffusion tensor imaging white matter tracts. The effort is being translated from 3T to newer field studies at 7T. OB

studies. Issues regarding the Opimal protOcol aNd sEt oF sEqueNce pArameters needeD For vArious diffusion studies Are unDeR InveSTiGatioN, which iNClUDEs mOrE rApiD pROtOcols For The asseSsment of stRokE aNd more high reSOlUtion protoColS for diffusioN teNsor imAgIng OF whitE maTter tRacts. THE efforT is being tRaNSlated FRom 3T to nEWEr FielD field studies at 7T. Ob

studies. Issues regarding  the opima l pro toc olan d se t of  sequence para m eter s needed for various d iffus io n  stu d ie s are  underi nv e s tig at io n,wh i ch  incl ude s morerapid prot oco ls  for the ass e ss ment of st rok e and more h igh  resol ut ion proto col s for  diffu s ion te nsor imag in g  of wh i te matt e r  t ract s. The effort isb ei n g translated f rom 3T  t o  n e w erfie ld field s tu diesa t 7T. O B

studies._Issues regarding_the opimal protocol and_set of_sequence_parameters needed_for_various diffusion studies_are under investigation,_which includes more rapid_protocols for the_assessment_of stroke and more high resolution protocols for diffusion tensor imaging of white matter_tracts._The effort_is_being_translated from 3T to newer_field field studies at 7T._OB


is for the Center, which is not necessarily the institution for the investigator. Polysaccharides are used in food and pharmaceutical applications due to their inherent abilities such as gelation, viscosity and thickening behavior. As the macroscopic properties of these biopolymers are dictated mainly by their molecular geometry and atomic level interactions, accurate molecular details

is for the Center, which is not necessarily the institution for the investigator. Polysaccharides are use in food and pharmaceutical application due to their inherent abilities such as gelation, viscosity and thickening demeanor. As the macroscopic properties of these biopolymers are dictated mainly by their molecular geometry and nuclear horizontal surface interactions, accurate molecular detail

is for the Center, which is not necessarili rhe invtitutjon for ghe investigator. Polysaccharmdes are ysed in food and pharmxceutical applicarionw due to thxjr inhevznt agllitizs such as gelatlon, viscosidy and thickenhne yehavior. As the macroscopic propertiqs of tnede biopolymers are qictzneb mainly by their molecular geojetry aid atomic level interactions, accurate molfculwr details

is for the Center, which is not institution the investigator. are used in to inherent abilities such gelation, viscosity and behavior. As the macroscopic properties of biopolymers are dictated mainly by their molecular geometry and atomic level interactions, accurate details

is for the Center, which is not nEcessarily The inStiTutIoN for The iNvestigator. PolYSaccHarides are used in food anD pharMaCEutiCAl AppliCations DUe TO TheIr InHerEnT AbIlitiEs sUch as geLation, viscOsiTy And thickeninG BeHavior. As thE maCroscopic proPerTies of ThEse BIopolYmeRs are DictatED mainlY by their mOlECular gEOmetry aND AtOmic Level interactions, ACcURate molecular dEtails

is for the Center, whichis not nec essar ily  th einst itut ion for the in v esti gator. Polysaccharides  areus e d in fo od an d pharm a ce u t ica lap pli ca t io ns du e t o their  inherentabi li ties such as ge lation, vi sco sity and thi cke ning b eh avi o r. As  th e mac roscop i c prop erties of  t h ese bi o polymer s ar e di ctated mainly byt he i r molecular ge ometry  a n da t omi c l evel inter ac tions ,  accura t em o l ecu l ar details

is_for the_Center, which is not_necessarily the_institution_for the_investigator._Polysaccharides are used_in food and_pharmaceutical applications due to_their inherent abilities_such_as gelation, viscosity and thickening behavior. As the macroscopic properties of these biopolymers are_dictated_mainly by_their_molecular_geometry and atomic level interactions,_accurate molecular details


associated with endometriosis. In the course of a study on the long-term effects of dioxin exposure, Drs. R. Bowman and S. Rier discovered that female monkeys exposed to either 5 or 25 ppt TCDD for 4 years were at greater risk for developing endometriosis as they aged (

associated with endometriosis. In the course of a study on the long - condition effect of dioxin exposure, Drs. R. Bowman and S. Rier discovered that female monkeys unwrap to either 5 or 25 ppt TCDD for 4 years were at greater hazard for developing endometriosis as they aged (

asdociated with endometrioris. In the course of a study on the uong-term effects of dioxin eeposyre, Des. R. Bowman and S. Rier discovervd that fwmalt monkeys exposed to eithcx 5 or 25 ppt VCDD for 4 years were at gseater risk fos aereloping endometriosis as they aged (

associated with endometriosis. In the course of on long-term effects dioxin exposure, Drs. discovered female monkeys exposed either 5 or ppt TCDD for 4 years were greater risk for developing endometriosis as they aged (

associated with endometriosIs. In the couRse of A stUdy On The lOng-tErm effects of diOXin eXposure, Drs. R. Bowman and S. RIer diScOVereD ThAt femAle monkEYs EXPosEd To EitHeR 5 Or 25 Ppt TCdD fOr 4 years Were at greaTer RiSk for developINg EndometrioSis As they aged (

associated with endometri osis. In t he co urs e o fa st udyon the long-te r m ef fects of dioxin exposu re, D rs .  R.B ow man a nd S. R i er d isc ov er edth a tfemal e m onkeysexposed to  ei th er 5 or 25 p p tTCDD for 4  ye ars were atgre ater r is k f o r dev elo pingendome t riosis  as theyag e d (

associated_with endometriosis._In the course of_a study_on_the long-term_effects_of dioxin exposure,_Drs. R. Bowman_and S. Rier discovered_that female monkeys_exposed_to either 5 or 25 ppt TCDD for 4 years were at greater risk_for_developing endometriosis_as_they_aged (


animal system. Previous work has shown that divergence in male and female mating behaviors leads to reproductive isolation between stickleback populations that have adapted to living in two different habitats ('benthic'and 'limnetic') in a single lake. There are multiple lakes in British Columbia in which these benthic and lim

animal system. Previous work has shown that deviation in male and female coupling behaviors leads to generative isolation between stickleback populations that have adjust to living in two different habitat (' benthic'and' limnetic') in a unmarried lake. There are multiple lakes in British Columbia in which these benthic and lim

anlmal system. Previous wory has shown thaj eivergxnce in male ana female mating behaviors leeds ro rekgoductive isolation bdtween stpckleback popnlations that hats adaptcb to mlving mn two differenj habitats ('banthic'and 'limnatkc') in a single lake. There are multiplq lakes ij British Coluibia yn wgpcm these benthic and lim

animal system. Previous work has shown that male female mating leads to reproductive have to living in different habitats ('benthic'and in a single lake. There are lakes in British Columbia in which these benthic and lim

animal system. Previous work hAs shown thaT diveRgeNce In Male And fEmale mating behAViorS leads to reproductive isOlatiOn BEtweEN sTicklEback poPUlATIonS tHaT haVe ADaPted tO liVing in tWo differenT haBiTats ('benthic'aND 'lImnetic') in a SinGle lake. There Are MultipLe LakES in BrItiSh ColUmbia iN Which tHese benthIc ANd lim

animal system. Previous w ork has sh own t hat  di ve rgen ce i n male and fem a le m ating behaviors leadsto re pr o duct i ve  isol ation b e tw e e n s ti ck leb ac k  p opula tio ns that  have adap ted  t o living int wo  different  ha bitats ('ben thi c'and'l imn e tic')  in  a si ngle l a ke. Th ere are m ul t iple l a kes inB r it ishColumbia in which th e se benthic and  lim

animal_system. Previous_work has shown that_divergence in_male_and female_mating_behaviors leads to_reproductive isolation between_stickleback populations that have_adapted to living_in_two different habitats ('benthic'and 'limnetic') in a single lake. There are multiple lakes in_British_Columbia in_which_these_benthic and lim


, and the results will be correlated with creep and fatigue behavior as determined in vitro under oxidative and hydrolytic conditions. 4) To determine the effect of PU surface chemistry and the effect of additives on the adhesion of polymorphonuclear leukocytes and monocytes/macrophages under static and dynamic conditions, with and without the presence of complement

, and the results will be correlated with creep and fatigue behavior as determine in vitro under oxidative and hydrolytic condition. 4) To determine the consequence of PU airfoil chemistry and the effect of additives on the attachment of polymorphonuclear leukocyte and monocytes / macrophages under static and dynamic conditions, with and without the bearing of complement

, anf the results will be covrelated with crgep and hatigue behaviof as determined in vitro undxr ozidatuve and hydrolytic conaitions. 4) No determune uhe effect of PU surface gkemisfvy anb vhe effect of acditives ot the adhesion ow 'olymorphonuclear leukocytes and monjcytes/mscgophages under stauic and dynamic conditions, with and withoht the kresence of complekent

, and the results will be correlated and behavior as in vitro under To the effect of surface chemistry and effect of additives on the adhesion polymorphonuclear leukocytes and monocytes/macrophages under static and dynamic conditions, with and without the of complement

, and the results will be correlAted with crEep anD faTigUe BehaVior As determined in VItro Under oxidative and hydroLytic CoNDitiONs. 4) to detErmine tHE eFFEct Of pU SurFaCE cHemisTry And the eFfect of addItiVeS on the adhesiON oF polymorphOnuClear leukocyTes And monOcYteS/MacroPhaGes unDer staTIc and dYnamic conDiTIons, wiTH and witHOUt The pResence of complemeNT

, and the results will becorrelated  with  cr eep  a nd f atig ue behavior as dete rmined in vitro underoxida ti v e an d  h ydrol ytic co n di t i ons .4)  To  d e te rmine  th e effec t of PU su rfa ce  chemistry a n dthe effect  of  additives o n t he adh es ion of po lym orpho nuclea r  leuko cytes and  m o nocyte s /macrop h a ge s un der static and dy n am i c conditions,with a nd wi t h out  th e presence  o f com p lement

, and_the results_will be correlated with_creep and_fatigue_behavior as_determined_in vitro under_oxidative and hydrolytic_conditions. 4) To determine_the effect of_PU_surface chemistry and the effect of additives on the adhesion of polymorphonuclear leukocytes and_monocytes/macrophages_under static_and_dynamic_conditions, with and without the_presence of complement


kinetic parameters in insulin triggered PIP3 signaling as well as in GLUT4 translocation. In the second part, we will execute, analyze and interpret a screen of the insulin-PIP3-GLUT4 signaling network with this new approach and will also perform a screen of cross-talk between insulin and TNF-alpha

kinetic parameters in insulin triggered PIP3 signaling equally well as in GLUT4 translocation. In the second character, we will execute, analyze and interpret a sieve of the insulin - PIP3 - GLUT4 bespeak network with this new access and will also perform a blind of cross - talk between insulin and TNF - alpha

kijetic parameters in insuuin triggered PNP3 signeling aa well ar in GLUT4 translocation. In tie swcond part, we will execute, xnalyze ajd interpret q screen oh the insulin-PJI3-GLUT4 wignaling netwprk with tvis new approawh aud will also perform a screen of crofs-talk nehween insulin wnd UNF-wlphz

kinetic parameters in insulin triggered PIP3 signaling as GLUT4 translocation. the second part, interpret screen of the signaling network with new approach and will also perform screen of cross-talk between insulin and TNF-alpha

kinetic parameters in insuliN triggered pIP3 siGnaLinG aS welL as iN GLUT4 translocaTIon. IN the second part, we will exEcute, AnALyze ANd InterPret a scREeN OF thE iNsUliN-Pip3-GlUT4 siGnaLing netWork with thIs nEw Approach and wILl Also perforM a sCreen of cross-TalK betweEn InsULin anD TNf-alphA

kinetic parameters in ins ulin trigg eredPIP 3 s ig nali ng a s well as in G L UT4translocation. In thesecon dp art, we  will  execut e ,a n aly ze  a ndin t er preta s creen o f the insu lin -P IP3-GLUT4 si g na ling netwo rkwith this ne w a pproac hand willals o per form a screen  of cross -t a lk bet w een ins u l in  and  TNF-alpha

kinetic_parameters in_insulin triggered PIP3 signaling_as well_as_in GLUT4_translocation._In the second_part, we will_execute, analyze and interpret_a screen of_the_insulin-PIP3-GLUT4 signaling network with this new approach and will also perform a screen of_cross-talk_between insulin_and_TNF-alpha


We show that mice with severe SCD exhibit evidence for ongoing pain and pronounced pain-like behavior in response to cold, mechanical and heat stimuli. Our preliminary data also indicate that sensory neurons from sickle mice have severely dysfunctional mitochondria. We hypothesize that ROS and oxidative stress induce mitochondrial dysfunction and TRP channel sensitization in

We show that mice with severe SCD exhibit evidence for ongoing annoyance and pronounce pain - like demeanor in response to cold, mechanical and estrus stimuli. Our preliminary datum besides indicate that sensory neurons from sickle mice have hard dysfunctional mitochondria. We hypothesize that ROS and oxidative tension induce mitochondrial dysfunction and TRP channel sensitization in

We show that mice with sevtre SCD exhibit erudence for ohgoing pxin and pronounced pain-like uehacior un response to cold, mezhanical wnd heat stinyli. Our prxmiminarn datz alsm indicate that sensory naurons from siwkue mice have severely dysfunctional mytochoncrla. We hypothesyze uhae ROA and oxidative stress induce mitodhondriel dysfunction snd TRP channel sensitizatlon ln

We show that mice with severe SCD for pain and pain-like behavior in heat Our preliminary data indicate that sensory from sickle mice have severely dysfunctional We hypothesize that ROS and oxidative stress induce mitochondrial dysfunction and TRP channel in

We show that mice with severe ScD exhibit eVidenCe fOr oNgOing Pain And pronounced pAIn-liKe behavior in response to Cold, mEcHAnicAL aNd heaT stimulI. ouR PRelImInAry DaTA aLso inDicAte that Sensory neuRonS fRom sickle micE HaVe severely DysFunctional miTocHondriA. WE hyPOthesIze That RoS and oXIdativE stress inDuCE mitocHOndrial DYSfUnctIon and TRP channel sENsITization in

We show that mice with se vere SCD e xhibi t e vid en ce f or o ngoing pain an d  pro nounced pain-like beha viorin resp o ns e tocold, m e ch a n ica lan d h ea t  s timul i.Our pre liminary d ata  a lso indicate th at sensory  ne urons from s ick le mic ehav e  seve rel y dys functi o nal mi tochondri a. We hyp o thesize t ha t RO S and oxidative s t re s s induce mitoc hondri al dy s f unc tio n and TRPch annel sensiti z at i o n  in

We_show that_mice with severe SCD_exhibit evidence_for_ongoing pain_and_pronounced pain-like behavior_in response to_cold, mechanical and heat_stimuli. Our preliminary_data_also indicate that sensory neurons from sickle mice have severely dysfunctional mitochondria. We hypothesize_that_ROS and_oxidative_stress_induce mitochondrial dysfunction and TRP_channel sensitization in


astructural analysis in situ by immersing the entire pouch in fixative. Thin cross sections of arterioles are cut, stained and examined with a TEM. The frequency and distribution of MEJ's present in both dilated and constricted microvessels will be compared. Morphometric analysis of vessel cross sections

astructural analysis in situ by immersing the entire pouch in fixative. Thin hybridization section of arterioles are cut, stained and examine with a TEM. The frequency and distribution of MEJ's present in both dilated and constricted microvessels will be compared. Morphometric analysis of vessel crisscross sections

astguctural analysis in sitm by immersing tkw entice poucg in fixxtive. Thin cross sections of aeteriiles are cut, stained avd examinvd with a TEM. Rhe frequeidy and distrigmtion if MEJ's presenj in both dinated and consdrkcced microvessels will be compared. Mowphomettif analysis of desstl srosa sections

astructural analysis in situ by immersing the in Thin cross of arterioles are a The frequency and of MEJ's present both dilated and constricted microvessels will compared. Morphometric analysis of vessel cross sections

astructural analysis in situ By immersinG the eNtiRe pOuCh in FixaTive. Thin cross sECtioNs of arterioles are cut, stAined AnD ExamINeD with A TEM. The FReQUEncY aNd DisTrIBuTion oF MEj's preseNt in both diLatEd And constrictED mIcrovesselS wiLl be compared. morPhometRiC anALysis Of vEssel Cross sECtions

astructural analysis in si tu by imme rsing  th e e nt irepouc h in fixative. Thin  cross sections of art eriol es arec ut , sta ined an d  e x a min ed  w ith  a TE M. Th e f requenc y and dist rib ut ion of MEJ's pr esent in b oth  dilated and  co nstric te d m i crove sse ls wi ll bec ompare d. Morpho me t ric an a lysis o f ve ssel  cross sections

astructural analysis_in situ_by immersing the entire_pouch in_fixative._Thin cross_sections_of arterioles are_cut, stained and_examined with a TEM._The frequency and_distribution_of MEJ's present in both dilated and constricted microvessels will be compared. Morphometric analysis_of_vessel cross_sections


a predictor of the extravasation of SLD, and therefore of the efficacy of SLD in treating a specific tumor. To test these hypotheses, NCTX was used to image rat mammary adenocarcinomas using a clinical mammography instrument. It was demonstrated that in individual rats bearing tumors of identical size and morphology, those

a predictor of the extravasation of SLD, and therefore of the efficacy of SLD in treating a specific tumor. To screen these guess, NCTX was used to image rat mammary adenocarcinomas use a clinical mammography instrument. It was demonstrated that in individual informer hold tumors of identical size and morphology, those

a oredictor of the extravaration of SLD, aue therxfore or the efwicacy of SLD in treating a dpwcifix tumor. To test these fypothesed, NCTX wqs uwwd to imagx rat mammary zfenoeaccinomas using s clinical mammography itsgrbment. It was demonstrated that in inqividuak gats bearing tomors jf isvnuical size and morphology, those

a predictor of the extravasation of SLD, of efficacy of in treating a hypotheses, was used to rat mammary adenocarcinomas a clinical mammography instrument. It was that in individual rats bearing tumors of identical size and morphology, those

a predictor of the extravasatIon of SLD, anD therEfoRe oF tHe efFicaCy of SLD in treatINg a sPecific tumor. To test thesE hypoThESes, NctX Was usEd to imaGE rAT MamMaRy AdeNoCArCinomAs uSing a clInical mammOgrApHy instrument. iT wAs demonstrAteD that in indivIduAl rats BeAriNG tumoRs oF idenTical sIZe and mOrphology, ThOSe

a predictor of the extrav asation of  SLD,  an d t he refo re o f the efficacy of S LD in treating a speci fic t um o r. T o  t est t hese hy p ot h e ses ,NC TXwa s  u sed t o i mage ra t mammaryade no carcinomas u s in g a clinic almammographyins trumen t.  It was d emo nstra ted th a t in i ndividual  r a ts bea r ing tum o r sof i dentical size and mo r phology, those

a_predictor of_the extravasation of SLD,_and therefore_of_the efficacy_of_SLD in treating_a specific tumor._To test these hypotheses,_NCTX was used_to_image rat mammary adenocarcinomas using a clinical mammography instrument. It was demonstrated that in_individual_rats bearing_tumors_of_identical size and morphology, those


the disease. Therefore, there is a dire need for the development of novel strategies by which pancreatic tumor progression could be prevented. The constitutive activation of EGFR and Akt signaling, commonly seen in PC, is known to activate NF-:B, which transcriptionally regulates many genes contributing to aggressive tumor growth, angiogenesis and invasion,

the disease. Therefore, there is a dire need for the growth of fresh strategies by which pancreatic tumor progression could be prevent. The constituent activation of EGFR and Akt signaling, commonly seen in PC, is know to activate NF-:B, which transcriptionally regulates many gene contribute to aggressive tumor growth, angiogenesis and invasion,

thf disease. Therefore, thert is a dire need yir the develkpment ow novel strategies by which 'anceeatix tumor progression cojld be prvvented. Tye cibstitutive activatljn or EGFX end Akt signalikg, commonly seen in PC, is kvocn to activate NF-:B, which transcriptijnally tehulates many ggnes bogtrigltlng to aggressive tumor growth, ahgiogentsis and invasion,

the disease. Therefore, there is a dire the of novel by which pancreatic The activation of EGFR Akt signaling, commonly in PC, is known to activate which transcriptionally regulates many genes contributing to aggressive tumor growth, angiogenesis and invasion,

the disease. Therefore, there iS a dire need For thE deVelOpMent Of noVel strategies bY WhicH pancreatic tumor progreSsion CoULd be PReVenteD. The conSTiTUTivE aCtIvaTiON oF EGFR And akt signAling, commoNly SeEn in PC, is knowN To Activate NF-:b, whIch transcripTioNally rEgUlaTEs manY geNes coNtribuTIng to aGgressive TuMOr growTH, angiogENEsIs anD invasion,

the disease. Therefore, t here is adirenee d f or  the  dev elopment of no v el s trategies by which pan creat ic tumo r  p rogre ssion c o ul d bepr ev ent ed .  T he co nst itutive  activatio n o fEGFR and Akt si gnaling, c omm only seen in  PC , is k no wnt o act iva te NF -:B, w h ich tr anscripti on a lly re g ulatesm a ny  gen es contributing t o  a g gressive tumor  growt h, an g i oge nes is and inv as ion,

the_disease. Therefore,_there is a dire_need for_the_development of_novel_strategies by which_pancreatic tumor progression_could be prevented. The_constitutive activation of_EGFR_and Akt signaling, commonly seen in PC, is known to activate NF-:B, which transcriptionally_regulates_many genes_contributing_to_aggressive tumor growth, angiogenesis and_invasion,


and neurodegenerative diseases. DESCRIPTION: Restoration of muscle function after injury requires the coordinate restoration of muscle fibers and microcirculation, as well as muscle and vascular innervation. Hypertension may have a significant effect on muscle regeneration, particularly on the regeneration of muscle blood vessels and the restoration of normal vascular control mechanisms.

and neurodegenerative diseases. DESCRIPTION: Restoration of muscle function after wound command the coordinate restoration of muscle fiber and microcirculation, equally well as muscle and vascular innervation. Hypertension may have a meaning impression on muscle positive feedback, particularly on the regeneration of brawn blood vessels and the renovation of normal vascular restraint mechanisms.

anf neurodegenerative disexses. DESCRIPTIOU: Restocation kf muscld function after injury requmres the xoordinate restoration of musclv fibers qnd nucrocirculefion, as well zd muvrle and vasculat innervatiot. Hypertension mxy have a significant effect on musclq regenrrwtion, particulwrly jn tgv vegeneration of muscle blood vesaels anv the restoratipn of normal vascular contgol lechanisms.

and neurodegenerative diseases. DESCRIPTION: Restoration of muscle injury the coordinate of muscle fibers muscle vascular innervation. Hypertension have a significant on muscle regeneration, particularly on the of muscle blood vessels and the restoration of normal vascular control mechanisms.

and neurodegenerative diseaSes. DESCRIPtION: REstOraTiOn of MuscLe function afteR InjuRy requires the coordinatE restOrATion OF mUscle Fibers aND mICRocIrCuLatIoN, As Well aS muScle and Vascular inNerVaTion. HypertenSIoN may have a sIgnIficant effecT on Muscle ReGenERatioN, paRticuLarly oN The regEneration Of MUscle bLOod vessELS aNd thE restoration of norMAl VAscular control MechanIsMS.

and neurodegenerative dis eases. DES CRIPT ION : R es tora tion  of muscle fun c tion  after injury requires  theco o rdin a te  rest oration of m usc le  f ibe rs an d mic roc irculat ion, as we llas  muscle andv as cular inne rva tion. Hypert ens ion ma yhav e  a si gni fican t effe c t on m uscle reg en e ration ,  partic u l ar ly o n the regeneratio n  o f  muscle bloodvessel sa nd t heres toration o fnorma l  vascul a rc o n tro l  mechanisms.

and_neurodegenerative diseases._DESCRIPTION: Restoration of muscle_function after_injury_requires the_coordinate_restoration of muscle_fibers and microcirculation,_as well as muscle_and vascular innervation._Hypertension_may have a significant effect on muscle regeneration, particularly on the regeneration of muscle_blood_vessels and_the_restoration_of normal vascular control mechanisms.


a quorum must be present. The CPRMC reviews all protocols executed under the auspices of the Cancer Center and which use VCC resources. The objectives of the proposed studies are to investigate the relationship between immune responsiveness and leukemogenesis in congenic strains of mice. One portion of study will be directed at evaluating

a quorum must be present. The CPRMC reviews all protocols executed under the auspices of the Cancer Center and which practice VCC resource. The objectives of the proposed report are to investigate the kinship between immune responsiveness and leukemogenesis in congenic strains of mice. One portion of cogitation will be directed at evaluating

a euorum must be present. Tme CPRMC reviews all prmtocola executdd under the auspices of the Cqncer Center and which use XCC resougces. The ibjertives of the proposed sbbdies wre co investigate tme relationvhip between ikmjnz responsiveness and leukemogenesis yn congrnlc strains of iice. Jne lortion of study will be directed zt evallating

a quorum must be present. The CPRMC protocols under the of the Cancer resources. objectives of the studies are to the relationship between immune responsiveness and in congenic strains of mice. One portion of study will be directed at

a quorum must be present. The CPrMC reviews All prOtoColS eXecuTed uNder the auspiceS Of thE Cancer Center and which uSe VCC ReSOurcES. THe objEctives OF tHE ProPoSeD stUdIEs Are to InvEstigatE the relatiOnsHiP between immuNE rEsponsivenEss And leukemogeNesIs in coNgEniC StraiNs oF mice. one porTIon of sTudy will bE dIRected AT evaluaTINg

a quorum must be present.  The CPRMC  revi ews  al lprot ocol s executed und e r th e auspices of the Canc er Ce nt e r an d  w hichuse VCC re s o urc es .The  o b je ctive s o f the p roposed st udi es  are to inve s ti gate the r ela tionship bet wee n immu ne  re s ponsi ven ess a nd leu k emogen esis in c on g enic s t rains o f mi ce.One portion of st u dy will be direct ed atev a lu a t ing

a_quorum must_be present. The CPRMC_reviews all_protocols_executed under_the_auspices of the_Cancer Center and_which use VCC resources._The objectives of_the_proposed studies are to investigate the relationship between immune responsiveness and leukemogenesis in congenic_strains_of mice._One_portion_of study will be directed_at evaluating


therapy in pediatric populations, and the prophylaxis and treatment of opportunistic infection in children. This program, consisting of a pediatric unit, an obstetrical unit and a virology core laboratory has a distinguished record over the past five years. The program faculty have provided scientific input into the conduct of clinical trials both nationally and locally

therapy in pediatric populations, and the prophylaxis and treatment of opportunistic infection in child. This platform, consisting of a pediatric unit, an obstetrical whole and a virology core laboratory have a distinguished record over the past five years. The program faculty have provided scientific stimulation into the conduct of clinical trials both nationally and locally

thfrapy in pediatric populxtions, and the kriphylaeis and treatmevt of opportunistic infectioi in chileren. This program, consksting of a pediarric ynit, an obstetrical unit wnd c tirology core lsboratory vas a distinguhsfeb record over the past five years. Thq progrsm faculty have krovicqd sdpektific input into the conduct of clinicel trials both mationally and locally

therapy in pediatric populations, and the prophylaxis of infection in This program, consisting obstetrical and a virology laboratory has a record over the past five years. program faculty have provided scientific input into the conduct of clinical trials both and locally

therapy in pediatric populatIons, and the ProphYlaXis AnD treAtmeNt of opportunisTIc inFection in children. This pRograM, cONsisTInG of a pEdiatriC UnIT, An oBsTeTriCaL UnIt and A viRology cOre laboratOry HaS a distinguisHEd Record over The Past five yearS. ThE progrAm FacULty haVe pRovidEd scieNTific iNput into tHe COnduct OF clinicAL TrIals Both nationally and LOcALly

therapy in pediatric popu lations, a nd th e p rop hy laxi s an d treatment of oppo rtunistic infection in  chil dr e n. T h is  prog ram, co n si s t ing  o fa p ed i at ric u nit , an ob stetricaluni tand a virolo g ycore labor ato ry has a dis tin guishe drec o rd ov erthe p ast fi v e year s. The pr og r am fac u lty hav e pr ovid ed scientific inp u ti nto the conduc t of c li n ic a l  tr ial s both nat io nally and loc a ll y

therapy_in pediatric_populations, and the prophylaxis_and treatment_of_opportunistic infection_in_children. This program,_consisting of a_pediatric unit, an obstetrical_unit and a_virology_core laboratory has a distinguished record over the past five years. The program faculty_have_provided scientific_input_into_the conduct of clinical trials_both nationally and locally


spinning disk confocal microscopy), with equivalent signal-to-noise ratio, which should aid in embryo lineaging. Initial results from this collaboration are under review. On the technical side, we are pursuing various instrumental approaches that should allow us to image with better 3D resolution (a weakness of SPIM compared to other technologies

spinning disk confocal microscopy), with equivalent signal - to - noise ratio, which should help in embryo lineaging. Initial result from this collaboration are under review. On the technical side, we are pursuing respective instrumental approaches that should admit us to visualize with better 3D resolution (a weakness of SPIM compared to other engineering

spijning disk confocal micruscopy), with equncalent signam-to-noise ratio, which should aid in elbeyo luneaging. Initial resulgs from tjis collqboretion are under csview. Ok the bechnncel side, we are kursuing varhous instrumendau cpproaches that should allow us to iiage wiyh better 3D resojutipg (a svajness of SPIM compared to othsr techiologies

spinning disk confocal microscopy), with equivalent signal-to-noise should in embryo Initial results from On technical side, we pursuing various instrumental that should allow us to image better 3D resolution (a weakness of SPIM compared to other technologies

spinning disk confocal microScopy), with eQuivaLenT siGnAl-to-NoisE ratio, which shoULd aiD in embryo lineaging. InitIal reSuLTs frOM tHis coLlaboraTIoN ARe uNdEr RevIeW. on The teChnIcal sidE, we are pursUinG vArious instruMEnTal approacHes That should alLow Us to imAgE wiTH bettEr 3D ResolUtion (a WEaknesS of SPIM coMpARed to oTHer techNOLoGies

spinning disk confocal mic roscopy),withequ iva le nt s igna l-to-noise rat i o, w hich should aid in emb ryo l in e agin g .Initi al resu l ts f rom  t hi s c ol l ab orati onare und er review.  On  t he technical si de, we are  pu rsuing vario usinstru me nta l  appr oac hes t hat sh o uld al low us to  i m age wi t h bette r 3D  res olution (a weakne s so f SPIM compare d to o th e rt e chn olo gies

spinning disk_confocal microscopy),_with equivalent signal-to-noise ratio,_which should_aid_in embryo_lineaging._Initial results from_this collaboration are_under review. On the_technical side, we_are_pursuing various instrumental approaches that should allow us to image with better 3D resolution_(a_weakness of_SPIM_compared_to other technologies


ic T cells derived from aged but not young thymi demonstrated high levels of IgM on their cell surface suggesting the possible presence of auto-antibodies on the surface thymocytes with advancing age. Whether there are autoantibodies targeting proteins on thymic subsets with advancing age remains to be determined. We are currently examining several gene pathways that

ic T cells derived from aged but not young thymus attest high levels of IgM on their cellular telephone surface suggesting the possible bearing of car - antibodies on the surface thymocytes with advancing age. Whether there are autoantibody targeting proteins on thymic subsets with advancing old age remains to be determined. We are currently examining several gene nerve pathway that

ic H cells derived from agea but not young thymi vemonstdated hieh levels of IgM on their cepl surfqce suggesting the posrible predence of auti-qntibodies on the surfacs thykicytes with adyancing age. Whether there afe autoantibodies targeting proteins jn thymoc subsets with wdvamsing age remains to be determined. We ade currtntly examining seferal gene pathways that

ic T cells derived from aged but thymi high levels IgM on their presence auto-antibodies on the thymocytes with advancing Whether there are autoantibodies targeting proteins thymic subsets with advancing age remains to be determined. We are currently examining gene pathways that

ic T cells derived from aged buT not young tHymi dEmoNstRaTed hIgh lEvels of IgM on thEIr ceLl surface suggesting the PossiBlE PresENcE of auTo-antibODiES On tHe SuRfaCe THyMocytEs wIth advaNcing age. WhEthEr There are autoANtIbodies tarGetIng proteins oN thYmic suBsEts WIth adVanCing aGe remaINs to be DetermineD. WE Are curREntly exAMInIng sEveral gene pathwayS ThAT

ic T cells derived from ag ed but not  youn g t hym idemo nstr ated high leve l s of  IgM on their cell sur facesu g gest i ng  thepossibl e  p r e sen ce  o f a ut o -a ntibo die s on th e surfacethy mo cytes with a d va ncing age.  Wh ether thereare  autoa nt ibo d ies t arg eting  prote i ns onthymic su bs e ts wit h  advanc i n gageremains to be det e rm i ned. We are cu rrentl ye xa m i nin g s everal gen epathw a ys that

ic T_cells derived_from aged but not_young thymi_demonstrated_high levels_of_IgM on their_cell surface suggesting_the possible presence of_auto-antibodies on the_surface_thymocytes with advancing age. Whether there are autoantibodies targeting proteins on thymic subsets with_advancing_age remains_to_be_determined. We are currently examining_several gene pathways that


difficulty in obtaining suitable clinical material for study. A highly specialized and independent Clinical Core has been designed for this AADRC program to remove the burden from investigators of the consuming tasks of human subject recruitment/characterization and bronchoscope/sample acquisition while simultaneously supplying all investigators with sufficient clinical samples to efficiently pursue the mechanistic studies that

difficulty in obtaining suitable clinical material for discipline. A highly specialized and autonomous Clinical Core has been designed for this AADRC program to absent the effect from investigators of the consuming job of human topic recruitment / characterization and bronchoscope / sample acquisition while simultaneously supplying all detective with sufficient clinical samples to efficiently pursue the mechanistic sketch that

divficulty in obtaining sultable clinical materian for atudy. A fighly specialized and indepxndebt Clunical Core has been ddsigned flr this QADRR program to remove the nbrden nrom nntestigators of jhe consuminc tasks of humdn sbbject recruitment/characterization agd bronvhlscope/sample asquixytioh while simultaneously supplying aml invevtigators witn sufficient clinical samppes ho efficiently purdue the mecyanifric studies ghat

difficulty in obtaining suitable clinical material for highly and independent Core has been to the burden from of the consuming of human subject recruitment/characterization and bronchoscope/sample while simultaneously supplying all investigators with sufficient clinical samples to efficiently pursue the studies that

difficulty in obtaining suitAble clinicAl matEriAl fOr StudY. A hiGhly specializeD And iNdependent Clinical Core Has beEn DEsigNEd For thIs AADRC PRoGRAm tO rEmOve ThE BuRden fRom InvestiGators of thE coNsUming tasks of HUmAn subject rEcrUitment/charaCteRizatiOn And BRonchOscOpe/saMple acQUisitiOn while siMuLTaneouSLy supplYINg All iNvestigators with sUFfICient clinical sAmples To EFfICIenTly Pursue the mEcHanisTIc studiES tHAT

difficulty in obtaining s uitable cl inica l m ate ri al f or s tudy. A highly spec ialized and independen t Cli ni c al C o re  hasbeen de s ig n e d f or  t his  A A DR C pro gra m to re move the b urd en  from invest i ga tors of th e c onsuming tas ksof hum an  su b jectrec ruitm ent/ch a racter ization a nd bronch o scope/s a m pl e ac quisition while s i mu l taneously supp lyingal l  i n v est iga tors withsu ffici e nt clin i ca l s amp l es to efficie ntly pursue the  mecha ni sti c  studi es th at

difficulty_in obtaining_suitable clinical material for_study. A_highly_specialized and_independent_Clinical Core has_been designed for_this AADRC program to_remove the burden_from_investigators of the consuming tasks of human subject recruitment/characterization and bronchoscope/sample acquisition while simultaneously_supplying_all investigators_with_sufficient_clinical samples to efficiently pursue_the mechanistic studies that


is then transmitted to the brain via a neuronal network. The group of diseases being studied is referred to as congenital stationary night blindness. They have defects in signal transmission between photoreceptors and the second neuron in the pathway. The studies in this proposal will characterize the nature of the defects and determine new proteins critical to function. [

is then transmitted to the brain via a neuronal network. The group of disease being study is referred to as congenital stationary night blindness. They get defect in signal transmission between photoreceptors and the second neuron in the nerve pathway. The studies in this proposal will characterize the nature of the defect and determine new protein critical to function. [

is then transmitted to the brain via a neorinal nxtwork. Fhe grouo of diseases being studied ms rwferrtb to as congenital sgationary night boindiess. They have dxrects ik sighwl txaismission betwegn photorece[tors and the vezoud neuron in the pathway. The studies in thix oroposal will sharssterjde the nature of the defects ans deterkine new protrins critical to function. [

is then transmitted to the brain via network. group of being studied is night They have defects signal transmission between and the second neuron in the The studies in this proposal will characterize the nature of the defects and new proteins critical to function. [

is then transmitted to the braIn via a neurOnal nEtwOrk. thE groUp of Diseases being sTUdieD is referred to as congeniTal stAtIOnarY NiGht blIndness. tHeY HAve DeFeCts In SIgNal trAnsMission Between phoTorEcEptors and the SEcOnd neuron iN thE pathway. The sTudIes in tHiS prOPosal WilL charActeriZE the naTure of the DeFEcts anD DetermiNE NeW proTeins critical to fuNCtIOn. [

is then transmitted to th e brain vi a a n eur ona lnetw ork.  The group ofd isea ses being studied is r eferr ed to a s  c ongen ital st a ti o n ary  n ig htbl i nd ness.  Th ey have  defects i n s ig nal transmis s io n betweenpho toreceptorsand  the s ec ond neuro n i n the  pathw a y. The  studiesin this p r oposalw i ll  cha racterize the nat u re of the defects  and d et e rm i n e n ewproteins c ri tical to func t io n . [

is_then transmitted_to the brain via_a neuronal_network._The group_of_diseases being studied_is referred to_as congenital stationary night_blindness. They have_defects_in signal transmission between photoreceptors and the second neuron in the pathway. The studies_in_this proposal_will_characterize_the nature of the defects_and determine new proteins critical_to function._[


Daphnia as a general toxicogenomic model species for assessing metal exposure and effects in natural field populations. Catecholamine neurotransmitters have been implicated in the pathophysiology of several neurological and psychiatric disorders such as schizophrenia, depressive illness and Parkinson's disease. The enzyme which catalyzes the first and rate limiting step in

Daphnia as a general toxicogenomic model species for assessing metallic element vulnerability and effects in natural field population. Catecholamine neurotransmitters have been implicated in the pathophysiology of respective neurological and psychiatric disorders such as schizophrenia, depressive illness and Parkinson's disease. The enzyme which catalyzes the first and pace specify step in

Daohnia as a general toxicugenomic model species for aasessing metal exposure and effects mn nqturao field populations. Cagecholamije neurorranwnitters hats been liplidwted mn the pathophyxiology of several neuronoeieal and psychiatric disorders such af schizppjrenia, depressyve pljnesa and Parkinson's disease. The enzyms which catalyzes thr first and rate limiting dtep in

Daphnia as a general toxicogenomic model species metal and effects natural field populations. in pathophysiology of several and psychiatric disorders as schizophrenia, depressive illness and Parkinson's The enzyme which catalyzes the first and rate limiting step in

Daphnia as a general toxicogeNomic model SpeciEs fOr aSsEssiNg meTal exposure and EFfecTs in natural field populaTions. caTEchoLAmIne neUrotranSMiTTErs HaVe BeeN iMPlIcateD in The pathOphysiologY of SeVeral neuroloGIcAl and psychIatRic disorders SucH as schIzOphREnia, dEprEssivE illneSS and PaRkinson's dIsEAse. The ENzyme whICH cAtalYzes the first and raTE lIMiting step in

Daphnia as a general toxi cogenomicmodel  sp eci es  for  ass essing metal e x posu re and effects in natu ral f ie l d po p ul ation s. Cate c ho l a min ene uro tr a ns mitte rshave be en implica ted  i n the pathop h ys iology ofsev eral neurolo gic al and  p syc h iatri c d isord ers su c h as s chizophre ni a , depr e ssive i l l ne ss a nd Parkinson's di s ea s e. The enzymewhichca t al y z esthe  first and  r ate l i mitings te p i n

Daphnia_as a_general toxicogenomic model species_for assessing_metal_exposure and_effects_in natural field_populations. Catecholamine neurotransmitters_have been implicated in_the pathophysiology of_several_neurological and psychiatric disorders such as schizophrenia, depressive illness and Parkinson's disease. The enzyme_which_catalyzes the_first_and_rate limiting step in


the human and mouse insulin receptor genes. Recently, we have characterized trans-acting factors that bind to a 26 base pair enhancer located 503-528 base pairs upstream from the translation start site. This enhancer accounts for a two- to threefold increase in the level of gene expression. Using electrophoretic mobility shift analysis, we

the human and mouse insulin receptor genes. Recently, we have qualify trans - acting divisor that bind to a 26 base pair foil located 503 - 528 base pairs upriver from the transformation start site. This enhancer accounts for a two- to threefold addition in the level of gene expression. Using electrophoretic mobility shift psychoanalysis, we

thf human and mouse insulik receptor genes. Recentny, we gave chafacterized trans-acting factocs tyat bund to a 26 base pair enfancer lobated 503-528 bawe peirs upstream from the tvcnslaflon scact site. This enmancer accognts for a two- tu chreefold increase in the level of gqne exptedsion. Using elgctrokhowetid mobility shift analysis, we

the human and mouse insulin receptor genes. have trans-acting factors bind to a 503-528 pairs upstream from translation start site. enhancer accounts for a two- to increase in the level of gene expression. Using electrophoretic mobility shift analysis, we

the human and mouse insulin reCeptor geneS. ReceNtlY, we HaVe chAracTerized trans-acTIng fActors that bind to a 26 base pAir enHaNCer lOCaTed 503-528 baSe pairs UPsTREam FrOm The TrANsLatioN stArt site. this enhancEr aCcOunts for a two- TO tHreefold inCreAse in the leveL of Gene exPrEssIOn. UsiNg eLectrOphoreTIc mobiLity shift AnALysis, wE

the human and mouse insul in recepto r gen es.  Re ce ntly , we  have characte r ized  trans-acting factorsthatbi n d to a26 ba se pair en h a nce rlo cat ed 50 3-528  ba se pair s upstream  fr om  the transla t io n start si te.  This enhanc eraccoun ts  fo r  a tw o-to th reefol d  incre ase in th el evel o f  gene e x p re ssio n. Using electrop h or e tic mobility s hift a na l ys i s , w e

the_human and_mouse insulin receptor genes._Recently, we_have_characterized trans-acting_factors_that bind to_a 26 base_pair enhancer located 503-528_base pairs upstream_from_the translation start site. This enhancer accounts for a two- to threefold increase in_the_level of_gene_expression._Using electrophoretic mobility shift analysis,_we


accurate indicators for the evaluation of the early response to therapy. Since bone matrix is the largest store of collagen, the invasion of bone by metastatic cancer, is accompanied by an elevated excretion of hydroxyproline (HYPRO) in urine. HYPRO is excreted as dialyzable and non-dialyzable forms

accurate indicators for the evaluation of the early response to therapy. Since bone matrix is the largest store of collagen, the invasion of bone by metastatic cancer, is play along by an exalted excretion of hydroxyproline (HYPRO) in urine. HYPRO is excreted as dialyzable and non - dialyzable forms

acfurate indicators for tht evaluation of tkw earlb respohse to tferapy. Since bone matrix is vhe oargewt store of collagen, tfe invasiln of bobe bb metastatic canrsr, is ageompahled bv en elevated exctetion of hygroxyproline (HFPFO) in urine. HYPRO is excreted as dialrzable snf non-dialyzablg forkf

accurate indicators for the evaluation of the to Since bone is the largest of by metastatic cancer, accompanied by an excretion of hydroxyproline (HYPRO) in urine. is excreted as dialyzable and non-dialyzable forms

accurate indicators for the eValuation oF the eArlY reSpOnse To thErapy. Since bone MAtriX is the largest store of coLlageN, tHE invASiOn of bOne by meTAsTATic CaNcEr, iS aCCoMpaniEd bY an elevAted excretIon Of HydroxyproliNE (HyPRO) in urinE. HYpRO is excreteD as DialyzAbLe aND non-dIalYzablE forms

accurate indicators for t he evaluat ion o f t heea rlyresp onse to therap y . Si nce bone matrix is the  larg es t  sto r eof co llagen, th e inv as io n o fb on e bymet astatic  cancer, i s a cc ompanied bya nelevated e xcr etion of hyd rox yproli ne  (H Y PRO)inurine . HYPR O  is ex creted as  d i alyzab l e and n o n -d ialy zable forms

accurate_indicators for_the evaluation of the_early response_to_therapy. Since_bone_matrix is the_largest store of_collagen, the invasion of_bone by metastatic_cancer,_is accompanied by an elevated excretion of hydroxyproline (HYPRO) in urine. HYPRO is excreted_as_dialyzable and_non-dialyzable_forms


intraluminal solubilization and distribution within micelles and vesicles of biliary compared to dietary cholesterol in humans and assess the impact of ezetimibe treatment on absorption of endogenous or exogenous cholesterol by assessing absorption of human contents in the biliary diverted, rat lymph fistula model. For each of these aims, subjects will be

intraluminal solubilization and distribution within micelles and vesicles of biliary compared to dietary cholesterol in humans and measure the impingement of ezetimibe treatment on absorption of endogenous or exogenous cholesterol by assessing assimilation of human contents in the biliary diverted, denounce lymph fistulous withers model. For each of these aims, subjects will be

inhraluminal solubilizatiok and distribution withmn micemles and vesicles of biliary comparev to dietqry cholesterol in humxns and adsess thw imkact of ezetimibe treatmekc on znsorpciin of endogenoos or exogenmus cholesteron cy assessing absorption of human contqnts in tje biliary divgrted, wat mjmkh fistula model. For each of thess aims, vubjects will be

intraluminal solubilization and distribution within micelles and biliary to dietary in humans and treatment absorption of endogenous exogenous cholesterol by absorption of human contents in the diverted, rat lymph fistula model. For each of these aims, subjects will be

intraluminal solubilizatioN and distriButioN wiThiN mIcelLes aNd vesicles of biLIary Compared to dietary choleSteroL iN HumaNS aNd assEss the iMPaCT Of eZeTiMibE tREaTment On aBsorptiOn of endogeNouS oR exogenous chOLeSterol by asSesSing absorptiOn oF human CoNteNTs in tHe bIliarY diverTEd, rat lYmph fistuLa MOdel. FoR Each of tHESe Aims, Subjects will be

intraluminal solubilizati on and dis tribu tio n w it hinmice lles and vesic l es o f biliary compared todieta ry chol e st erolin huma n sa n d a ss es s t he im pactofezetimi be treatme nton  absorptiono fendogenous  or  exogenous c hol estero lbya ssess ing  abso rption of hum an conten ts in the biliary d iv erte d, rat lymph fist u la model. For eac h of t he s ea i ms,  su bjects wil lbe

intraluminal_solubilization and_distribution within micelles and_vesicles of_biliary_compared to_dietary_cholesterol in humans_and assess the_impact of ezetimibe treatment_on absorption of_endogenous_or exogenous cholesterol by assessing absorption of human contents in the biliary diverted, rat_lymph_fistula model._For_each_of these aims, subjects will_be


-dimensional histograms; other correlations include flow cytometry data with high-resolution image analysis data. In addition, there is the potential use of this device for obtaining high-frequency chromosome spreads on a microscope slide. Under computer control, the device performs the cell deposition function. With a recently purchased computer-controlled microscope, we are

-dimensional histograms; other correlations include menstruation cytometry datum with high - resolution prototype psychoanalysis data. In addition, there is the likely manipulation of this device for obtaining eminent - frequency chromosome spreads on a microscope slide. Under calculator control, the device performs the cell deposition affair. With a recently purchased computer - controlled microscope, we are

-dimfnsional histograms; othev correlations iuxlude hlow cyfometry aata with high-resolution imaje abalysus data. In addition, thdre is thv potentiql uww of this vsvice for obtzlning iigh-frequency cmromosome s[reads on a miwruseope slide. Under computer control, thq devicr oerforms the cgll dtpofitikn function. With a recently purchaaed comkuter-controlled mivroscope, we are

-dimensional histograms; other correlations include flow cytometry high-resolution analysis data. addition, there is device obtaining high-frequency chromosome on a microscope Under computer control, the device performs cell deposition function. With a recently purchased computer-controlled microscope, we are

-dimensional histograms; otheR correlatiOns inCluDe fLoW cytOmetRy data with high-REsolUtion image analysis data. in addItIOn, thERe Is the PotentiAL uSE Of tHiS dEviCe FOr ObtaiNinG high-frEquency chrOmoSoMe spreads on a MIcRoscope sliDe. UNder computer ConTrol, thE dEviCE perfOrmS the cEll depOSition Function. WItH A recenTLy purchASEd CompUter-controlled micROsCOpe, we are

-dimensional histograms; o ther corre latio nsinc lu de f lowcytometry data with  high-resolution image  anal ys i s da t a.  In a ddition ,  t h e reis  t hepo t en tialuse  of thi s device f orob taining high - fr equency ch rom osome spread s o n a mi cr osc o pe sl ide . Und er com p uter c ontrol, t he device perform s th e ce ll deposition fun c ti o n. With a rece ntly p ur c ha s e d c omp uter-contr ol led m i croscop e ,w e are

-dimensional histograms;_other correlations_include flow cytometry data_with high-resolution_image_analysis data._In_addition, there is_the potential use_of this device for_obtaining high-frequency chromosome_spreads_on a microscope slide. Under computer control, the device performs the cell deposition function._With_a recently_purchased_computer-controlled_microscope, we are


In addition, the delivery of therapeutic agents via the blood supply is often impossible because the Blood-Brain barrier (BBB) protects the brain tissue from foreign molecules. Laboratory experiments have shown that focused ultrasound (FUS) beams can be used for noninvasive interventions. However, the utilization of FUS in the brain

In addition, the delivery of therapeutic agents via the rake provision is often impossible because the Blood - Brain barrier (BBB) protects the mind tissue from extraneous molecules. Laboratory experiment have shown that focused ultrasound (FUS) radio beam can be used for noninvasive interventions. However, the use of FUS in the brain

In addition, the delivery on therapeutic aggnrs via the bmood supoly is often impossible becanse rhe Boood-Brain barrier (BBB) orotects nhe brain tiswye from focsign moleculea. Labmcatory experimekts have shmwn that focusad uptrasound (FUS) beams can be used for noninvsslve interventijns. Njwevsg, uhe utilization of FUS in the brajn

In addition, the delivery of therapeutic agents blood is often because the Blood-Brain tissue foreign molecules. Laboratory have shown that ultrasound (FUS) beams can be used noninvasive interventions. However, the utilization of FUS in the brain

In addition, the delivery of thErapeutic aGents Via The BlOod sUpplY is often impossIBle bEcause the Blood-Brain barRier (BbB) PRoteCTs The brAin tissUE fROM foReIgN moLeCUlEs. LabOraTory expEriments haVe sHoWn that focuseD UlTrasound (FUs) beAms can be used For NoninvAsIve INtervEntIons. HOwever, THe utilIzation of fUs In the bRAin

In addition, the delivery  of therap eutic  ag ent sviatheblood supply i s  oft en impossible becausethe B lo o d-Br a in  barr ier (BB B )p r ote ct sthe  b r ai n tis sue  from f oreign mol ecu le s. Laborator y  e xperiments  ha ve shown tha t f ocused  u ltr a sound  (F US) b eams c a n be u sed for n on i nvasiv e  interv e n ti ons.  However, the uti l iz a tion of FUS in  the b ra i n

In_addition, the_delivery of therapeutic agents_via the_blood_supply is_often_impossible because the_Blood-Brain barrier (BBB)_protects the brain tissue_from foreign molecules._Laboratory_experiments have shown that focused ultrasound (FUS) beams can be used for noninvasive interventions._However,_the utilization_of_FUS_in the brain


olerability, effect size, etc.) to design a larger RCT of low-dose naltrexone and/or nalmefene for chronic pain among persons with heavy alcohol use. We choose to conduct this research in St. Petersburg, Russia, given that: 1) nalmefene is licensed in Russia

olerability, effect size, etc .) to design a larger RCT of low - venereal disease naltrexone and/or nalmefene for chronic annoyance among persons with heavy alcohol habit. We choose to lead this research in St. Petersburg, Russia, given that: 1) nalmefene is license in Russia

olegability, effect size, etc.) to design a latgwr RCT of los-dose nautrexone and/or nalmefene for cyronix pain among persons wkth heavy alcohol use. Qe choose vk condugc thia reszacch in St. Peterxburg, Russha, given that: 1) nxllefene is licensed in Russia

olerability, effect size, etc.) to design a of naltrexone and/or for chronic pain use. choose to conduct research in St. Russia, given that: 1) nalmefene is in Russia

olerability, effect size, etc.) tO design a laRger RcT oF loW-dOse nAltrExone and/or nalmEFene For chronic pain among perSons wItH HeavY AlCohol Use. We chOOsE TO coNdUcT thIs REsEarch In ST. PetersBurg, Russia, GivEn That: 1) nalmefenE Is Licensed in rusSia

olerability, effect size,etc.) to d esign  alar ge r RC T of  low-dose nalt r exon e and/or nalmefene for  chro ni c  pai n  a mongpersons wi t h  he av yalc oh o luse.Wechooseto conduct  th is  research in St . Petersbu rg,  Russia, giv enthat:1)  na l mefen e i s lic ensedi n Russ ia

olerability, effect_size, etc.)_to design a larger_RCT of_low-dose_naltrexone and/or_nalmefene_for chronic pain_among persons with_heavy alcohol use. We_choose to conduct_this_research in St. Petersburg, Russia, given that: 1) nalmefene is licensed in Russia


integrated health care organizations. The PCIP is evaluating trends in priority quality measures (e.g., blood pressure and lipid control) among 90 small practices randomized to receive clinical decision support tools and/or financial rewards. This proposed study of 60 comparison practices that have not adopted EHRs will provide a unique opportunity to demonstrate

integrated health care organizations. The PCIP is evaluating drift in precedence quality measures (for example, blood imperativeness and lipid control) among 90 little practices randomized to pick up clinical decision support cock and/or financial rewards. This aim study of 60 comparison practices that have not adopted EHRs will provide a unique opportunity to demonstrate

inhegrated health care orgxnizations. The KCUP is xvaluatjng trenas in priority quality measuces (w.g., bliod pressure and lipid control) wmong 90 snall practices czndomizcb to dcceivz rlinical decisipn support tools and/or fhnxneial rewards. This proposed study of 60 comparosln practices trat nwve hot adopted EHRs will provide a unjque opkortunity to demonxtrate

integrated health care organizations. The PCIP is in quality measures blood pressure and practices to receive clinical support tools and/or rewards. This proposed study of 60 practices that have not adopted EHRs will provide a unique opportunity to demonstrate

integrated health care organIzations. ThE PCIP Is eValUaTing TrenDs in priority quALity Measures (e.g., blood pressurE and lIpID conTRoL) amonG 90 small pRAcTICes RaNdOmiZeD To ReceiVe cLinical Decision suPpoRt Tools and/or fiNAnCial rewardS. ThIs proposed stUdy Of 60 compArIsoN PractIceS that Have noT AdopteD EHRs will PrOVide a uNIque oppORTuNity To demonstrate

integrated health care or ganization s. Th e P CIP  i s ev alua ting trends in prio rity quality measures(e.g. ,b lood pr essur e and l i pi d con tr ol ) a mo n g90 sm all  practi ces random ize dto receive c l in ical decis ion  support too lsand/or  f ina n cialrew ards.  Thisp ropose d study o f6 0 comp a rison p r a ct ices  that have not ad o pt e d EHRs will pr ovideau ni q u e o ppo rtunity to  d emons t rate

integrated_health care_organizations. The PCIP is_evaluating trends_in_priority quality_measures_(e.g., blood pressure_and lipid control)_among 90 small practices_randomized to receive_clinical_decision support tools and/or financial rewards. This proposed study of 60 comparison practices that_have_not adopted_EHRs_will_provide a unique opportunity to_demonstrate


4) develop efficient path methods to study chemical mechanisms and conformational transitions in ribozymes. In this way we hope to greatly extend the predictive capability and range of application of state of the art theoretical methods to RNA catalysis. PUBLIC HEALTH RELEVANCE: The goal of this proposal is to use quantum mechanical and molecular

4) develop efficient path methods to analyze chemical mechanism and conformational transitions in ribozymes. In this way we hope to greatly extend the predictive capability and image of application of state of the artwork theoretical methods to RNA catalysis. PUBLIC HEALTH RELEVANCE: The goal of this proposal is to practice quantum mechanical and molecular

4) degelop efficient path metmods to study chgmucal mxchanisjs and cunformational transitions in rubozynes. In this way we hopd to greanly extene tht predictive capaujlity akb ranfc of cp'lication of stste of the art theoreticdl mzthods to RNA catalysis. PUBLIC HEALTR RELEVSNFE: The goal of thix prolosal is to use quantum mechanical and monecular

4) develop efficient path methods to study and transitions in In this way the capability and range application of state the art theoretical methods to RNA PUBLIC HEALTH RELEVANCE: The goal of this proposal is to use quantum mechanical molecular

4) develop efficient path methoDs to study cHemicAl mEchAnIsms And cOnformational tRAnsiTions in ribozymes. In this Way we HoPE to gREaTly exTend the PReDICtiVe CaPabIlITy And raNge Of appliCation of stAte Of The art theoreTIcAl methods tO RNa catalysis. PUbLIc HEALTh ReLEvaNCE: THe gOal of This prOPosal iS to use quaNtUM mechaNIcal and MOLeCulaR

4) develop efficient pathmethods to  stud y c hem ic al m echa nisms and conf o rmat ional transitions in r ibozy me s . In th is wa y we ho p et o  gr ea tl y e xt e nd  thepre dictive  capabilit y a nd  range of ap p li cation ofsta te of the ar t t heoret ic alm ethod s t o RNA  catal y sis. P UBLIC HEA LT H  RELEV A NCE: Th e go al o f this proposal i s  t o  use quantum m echani ca l  a n d  mo lec ular

4) develop_efficient path_methods to study chemical_mechanisms and_conformational_transitions in_ribozymes._In this way_we hope to_greatly extend the predictive_capability and range_of_application of state of the art theoretical methods to RNA catalysis. PUBLIC HEALTH RELEVANCE:_The_goal of_this_proposal_is to use quantum mechanical_and molecular


that can be considered appropriate for the K08 award. The career development plan is outlined for five years, with courses in epidemiology and biostatistics during phase I, followed by the period of independent research in phase II. The proposed research addresses the role of interferon in infection by dengue virus (DV), both to investigate how

that can be considered appropriate for the K08 award. The career development plan is delineate for five days, with courses in epidemiology and biostatistics during phase I, followed by the menstruation of independent research in phase II. The nominate inquiry addresses the role of interferon in infection by dengue virus (DV), both to investigate how

thwt can be considered appvopriate for the K08 awarv. The czreer dexelopment plan is outlined flr five years, with courses in epidemiopogy and biowratistics vhring pmcse I, nollocev by the period of indepetdent research iv 'hase II. The proposed research addrefses thr gole of interfgron pn infsbtlon by dengue virus (DV), both to ihvestigete how

that can be considered appropriate for the The development plan outlined for five and during phase I, by the period independent research in phase II. The research addresses the role of interferon in infection by dengue virus (DV), both investigate how

that can be considered appropRiate for thE K08 awaRd. THe cArEer dEvelOpment plan is ouTLineD for five years, with coursEs in ePiDEmioLOgY and bIostatiSTiCS DurInG pHasE I, FOlLowed By tHe perioD of indepenDenT rEsearch in phaSE Ii. The proposEd rEsearch addreSseS the roLe Of iNTerfeRon In infEction BY denguE virus (DV), bOtH To inveSTigate hOW

that can be considered ap propriatefor t heK08  a ward . Th e career devel o pmen t plan is outlined for  five  y e ars, wi th co urses i n  e p i dem io lo gyan d  b iosta tis tics du ring phase  I,  f ollowed by t h eperiod ofind ependent res ear ch inph ase II. T hepropo sed re s earchaddresses  t h e role of inte r f er on i n infection by de n gu e  virus (DV), b oth to  i n ve s t iga tehow

that_can be_considered appropriate for the_K08 award._The_career development_plan_is outlined for_five years, with_courses in epidemiology and_biostatistics during phase_I,_followed by the period of independent research in phase II. The proposed research addresses_the_role of_interferon_in_infection by dengue virus (DV),_both to investigate how


so the drug names were subject to variation and typos. Surescripts data provided standardized names. To make the two sources comparable, we mapped all drugs to their standard names in RxNorm, the U.S. standard reference drug terminology. Mapping was done largely by automatic text matching algorithms followed by manual review

so the drug names were subject to variation and typos. Surescripts datum provide standardized names. To produce the two source comparable, we mapped all drugs to their standard name in RxNorm, the U.S. standard reference book drug terminology. Mapping was act largely by automatic text matching algorithm followed by manual review

so the drug names were submect to variatiou and tbpos. Sudescriptr data provided standardized nqmes. Uj make the two soufces compwrable, ww makped all drugs to their sbcndars namzs in RxNorm, the U.S. standasd reference dsue cerminology. Mapping was done largely by autpmwtic text matcring wlgodptmms followed by manual review

so the drug names were subject to typos. data provided names. To make mapped drugs to their names in RxNorm, U.S. standard reference drug terminology. Mapping done largely by automatic text matching algorithms followed by manual review

so the drug names were subject To variatioN and tYpoS. SuReScriPts dAta provided staNDardIzed names. To make the two sOurceS cOMparABlE, we maPped all DRuGS To tHeIr StaNdARd Names In RXNorm, thE U.S. standarD reFeRence drug terMInOlogy. MappiNg wAs done largelY by AutomaTiC teXT matcHinG algoRithms FOlloweD by manual ReVIew

so the drug names were su bject to v ariat ion  an dtypo s. S urescripts dat a  pro vided standardized nam es. T om aket he  twosources co m p ara bl e,  we  m a pp ed al l d rugs to  their sta nda rd  names in Rx N or m, the U.S . s tandard refe ren ce dru gter m inolo gy.  Mapp ing wa s  donelargely b ya utomat i c textm a tc hing  algorithms follo w ed by manual revi ew

so_the drug_names were subject to_variation and_typos._Surescripts data_provided_standardized names. To_make the two_sources comparable, we mapped_all drugs to_their_standard names in RxNorm, the U.S. standard reference drug terminology. Mapping was done largely_by_automatic text_matching_algorithms_followed by manual review


VLM will be determined through the use of antidromic microstimulation and/or spike-triggered averaging of recorded field potentials. Second, monosynaptic connections between caudal and rostral VLM neurons will be investigated by recording simultaneous activity from pairs of these neurons and application of cross correlation analysis. Third

VLM will be determined through the use of antidromic microstimulation and/or spike - triggered averaging of recorded sphere potential. Second, monosynaptic connections between caudal and rostral VLM neurons will be investigated by record simultaneous activity from pair of these nerve cell and application of cross correlation analysis. Third

VLL will be determined thruugh the use of antidrmmic mjcrostimjlation and/or spike-triggered aceragung of recorded field ootentiald. Second, moniwynaptic connectiokf befaeen raudal and rosttal VLM neurmns will be ineertngated by recording simultaneous actyvity ftol pairs of thefe nturjns znd application of cross correlatikn analjsis. Third

VLM will be determined through the use microstimulation spike-triggered averaging recorded field potentials. and VLM neurons will investigated by recording activity from pairs of these neurons application of cross correlation analysis. Third

VLM will be determined througH the use of aNtidrOmiC miCrOstiMulaTion and/or spike-TRiggEred averaging of recordeD fielD pOTentIAlS. SecoNd, monosYNaPTIc cOnNeCtiOnS BeTween CauDal and rOstral VLM nEurOnS will be invesTIgAted by recoRdiNg simultaneoUs aCtivitY fRom PAirs oF thEse neUrons aND appliCation of cRoSS correLAtion anALYsIs. ThIrd

VLM will be determined th rough theuse o f a nti dr omic  mic rostimulationa nd/o r spike-triggered aver aging  o f  rec o rd ed fi eld pot e nt i a ls.  S ec ond ,m on osyna pti c conne ctions bet wee ncaudal and r o st ral VLM ne uro ns will be i nve stigat ed  by recor din g sim ultane o us act ivity fro mp airs o f  thesen e ur onsand application o f  c r oss correlatio n anal ys i s. T hir d

VLM_will be_determined through the use_of antidromic_microstimulation_and/or spike-triggered_averaging_of recorded field_potentials. Second, monosynaptic_connections between caudal and_rostral VLM neurons_will_be investigated by recording simultaneous activity from pairs of these neurons and application of_cross_correlation analysis._Third


gonorrhoeae causes about 800,000 new infections each year in the United States, with health-care costs approaching 2 billion dollars/year. Various surface components, including lipooligosaccharide (LOS) and opacity protein(s) (Opa(s)), are important in mediating disease. The objectives

gonorrhoeae causes about 800,000 new infections each class in the United States, with health - caution costs approaching 2 billion dollar / class. Various surface components, include lipooligosaccharide (LOS) and opacity protein(s) (Opa(s) ), are important in mediating disease. The aim

gojorrhoeae causes about 800,000 kew infections ecxh yeac in ths United States, with health-care costd qpproqching 2 billion dollarr/year. Varpous surfqce romponents, incluvjng lipooligoawcchcrmde (LOS) and opagity proteit(s) (Opa(s)), are im[oftcnt in mediating disease. The objectides

gonorrhoeae causes about 800,000 new infections each the States, with costs approaching 2 including (LOS) and opacity (Opa(s)), are important mediating disease. The objectives

gonorrhoeae causes about 800,000 new Infections Each yEar In tHe unitEd StAtes, with health-CAre cOsts approaching 2 billion DollaRs/YEar. VARiOus suRface coMPoNENts, InClUdiNg LIpOoligOsaCcharidE (LOS) and opaCitY pRotein(s) (Opa(s)), aRE iMportant in MedIating diseasE. ThE objecTiVes

gonorrhoeae causes about800,000 ne w inf ect ion seach  yea r in the Unite d  Sta tes, with health-carecosts  a p proa c hi ng 2billion do l l ars /y ea r.Va r io us su rfa ce comp onents, in clu di ng lipooligo s ac charide (L OS)  and opacity  pr otein( s)  (O p a(s)) , a re im portan t  in me diating d is e ase. T h e objec t i ve s

gonorrhoeae_causes about_800,000 new infections each_year in_the_United States,_with_health-care costs approaching_2 billion dollars/year._Various surface components, including_lipooligosaccharide (LOS) and_opacity_protein(s) (Opa(s)), are important in mediating disease. The objectives