bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementation of BERSABAR Method for Students to Achieve Clean and Healthy Schools in Al Ikhlas Private Elementary School Medan
Authors: Tumanggor Winni R. E. , Chahaya Indra , Siregar Cholina Trisa 
Published: January 2024

Sanitation conditions in the school environment in Urban Village Sitirejo II were not well managed. The school had adequate facilities, but these facilities were not optimally utilized as seen from the unavailability of clean water, unclean toilets, and scattered garbage. These conditions can lead to diseases such as diarrhea and others. The formation of cadres from students needs to be done to overcome these problems. The cadre was named Bocah Cinta Lingkungan or "Bocil" or the Environmentalist Child Cadres, 10 people were selected and formed through "Bersabar" method. The play method used is with the modification of the Snakes and Ladders game, the game is communicative and easy to understand, with eye-catching visualization, attractive, and fun to use as learning media. In addition, snakes and ladders are modified with interesting and full-color illustrated questions and answers. After the cadres are formed, their role is to instill clean and healthy living behaviors for themselves and their peers. Promotive efforts in school children groups can be done with peer education. Furthermore, this program also involves principals and teachers by providing a "Bersabar" method tools to Al Ikhlas Private elemntary School. Teachers have a role to monitor the cadres and then also be able to form the cadres independently.
PEMBELAJARAN READ, ANSWER, DISCUSS, EXPLAIN, CREATE (RADEC) BERBANTUAN APLIKASI QUIZIZZ UNTUK MENINGKATKAN HASIL BELAJAR SISWA SMP ISLAM SAMARGALILA HALMAHERA SELATAN
Authors: Ibrahim Harun , Taher Dharmawaty M. , Haerullah Ade 
Published: January 2024

Model pembelajaran RADEC berbantuan aplikasi Quiziz merupakan model pembelajaran yang di modifikasi untuk meningkatkan hasil belajar dan motivasi belajar dalam memecahkan permasalahan pembelajaran yang belum sesuai dengan tuntutan tujuan pendidikan nasional pada keterampilan abad 21. Tujuan Penelitian ini untuk mengetahui pengaruh penggunaan model pembelajaaran RADEC berbatuan aplikasi Quizizz terhadap hasil belajar siswa SMP Islam Samargalila Labuha dan mengetahui hubungan antara motivasi dengan hasil belajar siswa setelah menggunakan model pembelajaran RADEC berbantuan aplikasi Quiziz. Jenis penelitian ini yaitu Quasy eksperimen dengan Design type Nonequivalent Control Group Design. Penelitian ini dilaksanakan di SMP Islam Samargalila Labuha Kecamatan Bacan Kabupaten Halmahera Selatan, dilaksanakan pada tahun ajaran 2022/2023. Sampel dalam penelitian ini adalah siswa kelas IX-1 sebagai kelas eksperimen dan kelas IX-2 sebagai kelas kontrol. Teknik pengumpulan data yang digunakan adalah tes. Analisis data menggunakan uji normalitas dan homogenitas berbantuan SPSS sedangkan uji hipotesis mengunakan uji anacova dan Uji Korelasi. Hasil penelitian Menujukkan bahwa terdapat pengaruh penggunaan model pembelajaaran RADEC berbatuan aplikasi quizizz terhadap hasil belajar siswa dengan tafar signifikan sebesar 0,000 juga terdapat hubungan antara motivasi dengan hasil belajar siswa setelah menggunakan model pembelajaran RADEC berbatuan Aplikasi Quiziz pada siswa pada tafar signifikan sebesar 0,002 (lebih kecil dari 0,05) dengan koefisien korelasi 0,431.
Arc flash assessment in a small hydropower plant
Authors: Morales Jose Isaul , Bute H José J , Cárdenas Dorindo Elam 
Published: January 2024

El arc flash ocurre por el paso repentino de la corriente, debido a la ruptura súbita de la barrera dieléctrica del aire ionizado a través de los polos energizados. Durante este fenómeno, se libera una gran cantidad de energía en forma de calor y el personal de mantenimiento expuesto directamente a este evento está sujeto a quemaduras de tercer grado, ceguera, golpes, pérdida auditiva e incluso la muerte. El objetivo de este trabajo es mostrar una metodología práctica para el cálculo de la energía incidente a la distancia de trabajo y la frontera externa de un arc flash en un cuarto de media tensión con celdas operando con un nivel de voltaje de 34.5 kV. Las normas IEEE 1584.1-2022, IEEE 1584-2018, IEC 60947 e IEC62271 no cubren los cálculos de arc flash para tensiones mayores a 15kV, por lo tanto, para lograr el objetivo planteado, vamos a utilizar el método de Ralph Lee y luego utilizaremos un software para verificar los resultados obtenidos. También presentaremos el cálculo por medio de la aplicación del método de campos electromagnéticos. Analizando los resultados obtenidos, concluimos que para nuestro caso de estudio se requiere que todo el personal que ingrese a realizar trabajos con los equipos en funcionamiento debe contar con equipo de protección categoría 4 de acuerdo con la norma NFPA 70- 2018 para rango de energía incidente entre 25 y 40 cal/cm2. En Panamá hay gran cantidad de instalaciones con voltajes de operación por encima de 15 kV, por lo tanto, se hace necesario realizar más investigaciones y cálculos en estos lugares para garantizar la seguridad de los trabajadores ante el riesgo potencial de arc flash.
Upaya Meningkatkan Kreativitas dan Hasil Belajar Menggunakan Model Discovery learning Mata Pelajaran IPA
Authors: Muizzah Izza , Fatkhiyani Kiki 
Published: January 2024

AbstrakBerdasarkan latar belakang sebuah penelitian ditemukan rendahnya nilai hasil belajar dan rendahnya kreativitas siswa kelas V MI Raudlatus Shibyan. Rendahnya kreativitas dan hasil belajar disebabkan oleh masih kurangnya motivasi belajar dan guru cenderung menggunakan model pembelajaran konvensional seperti halnya menggunakan metode ceramah, tanya jawab dan pemberian tugas dalam pembelajaran. Penelitian ini menggunakan model Penelitian Tindakan Kelas (PTK) yang terdiri dari empat tahapan. Kreativitas siswa pada siklus I mencapai 76,13%, pada siklus II 82,95, artinya mengalami peningkatan. Hasil belajar pada siklus I sebesar 63,63%, pada siklus II mengalami peningkatan 81,81%, artinya ketuntasan hasil belajar siswa telah mencapai ketuntasan klasikal yang diharapkan yaitu 80%. Sehingga dapat disimpulkan bahwa model discovery learning dapat meningkatkan kreativitas dan hasil belajar siswa. Kata Kunci:KreativitasHasil BelajarDiscovery learning
Case Report: A family history of peanut allergy and hereditary alpha-tryptasemia
Authors: Chantran Yannick , Renaudin Hélène , Arock Michel , Guiddir Tamazoust , Nemni Ariane 
Published: January 2024

ContextHereditary alpha-tryptasemia (HαT) is associated with elevated basal serum tryptase (bST) and is associated with a higher risk of severe anaphylactic reactions in patients with clonal mast cell disorders or IgE-mediated Hymenoptera venom-induced anaphylaxis. The consequence of this genetic trait remains to be determined in other allergic diseases and food allergy in particular.ObjectivesHere, we describe three cases of peanut allergy among siblings from a single family of four: two of them were associated with HαT, and the third one was associated with the tryptase wild-type genotype.MethodsTPSAB1/TPSB2 genotypes were determined by digital PCR. After the case description, we provided a review of the literature regarding bST levels and tryptase genotypes in anaphylaxis, with a particular focus on food allergy.ResultsCompared to the sibling with the conventional tryptase genotype, the two siblings with HαT presented a lower peanut threshold at the initial oral food challenge, higher peanut skin prick test reactivity, higher levels of specific IgE to peanut, Ara h 2, and Ara h 6, and a lower IgG4/IgE ratio after 10 years of oral immunotherapy.ConclusionThe tryptase genotype and HαT status might modify the clinical presentation and biological features of food allergy.
Causal association between genetically predicted circulating immune cell counts and frailty: a two-sample Mendelian randomization study
Authors: Guo Xiao-Guang , Zhang Ya-Juan , Lu Ya-Xin , Lu Jia-Mei , Zhang Jie , Li Hui-Xin , Chen Chao-Jin , Yang Jian-Jun 
Published: January 2024

BackgroundDespite the recognized link between immune responses and frailty, the association between immune cell counts and frailty based on previous observational studies remains disputed, with uncertain causal nexus. This study aimed to elucidate causal association between genetically predicted circulating immune cell counts and frailty.MethodsWe conducted the two-sample Mendelian randomization (MR) study with independent genetic variants associated with six immune cell subtype counts from genome-wide association studies in 563,946 European individuals. Frailty summary data, assessed via frailty index (FI), was obtained from study comprising 175,226 subjects. Univariate MR, reverse MR and multivariate MR were conducted to comprehensive investigate the association between immune cell counts and FI, with two-step MR analysis for mediation analysis.ResultsUnivariate MR evidence indicated that among six leukocyte subtype counts, only elevated eosinophil count was significantly correlated with higher FI (β = 0.059, 95% confidence interval [CI], 0.042–0.078, P=5.63E-11), with no reverse causal relationship identified in reverse MR. In multivariate MR, the causal effect of eosinophil count retained statistical significance (β = 0.063, 95% CI, 0.021–0.104, P = 0.003). Ultimately, the two-step MR analysis demonstrated two mediators in this causal pathway: asthma (β= 0.019, 95% CI, 0.013–0.025, P = 35.84E-10, mediated proportion, 31.732%) and rheumatoid arthritis (β= 0.004, 95% CI, 0.001–0.006, P=1.75E-03, mediated proportion, 6.411%).ConclusionsWithin immune cell subtypes, MR evidence indicated only genetically predicted circulating eosinophil count had irreversible and independent causal effect on frailty, with asthma and rheumatoid arthritis possibly serving as partial mediators. The finding stressed the need for further exploring physiological functions of eosinophils in order to develop effective strategies against frailty.
Poor prognostic factors of pharmacokinetic origin predict outcomes in inflammatory bowel disease patients treated with anti-tumor necrosis factor-α
Authors: Spencer Elizabeth A. , Dubinsky Marla C. , Kamm Michael A. , Chaparro Maria , Gionchetti Paolo , Rizzello Fernando , Gisbert Javier P. , Wright Emily K. , Schulberg Julien D. , Hamilton Amy L. , McGovern Dermot P. B. , Dervieux Thierry 
Published: January 2024

IntroductionWe evaluated baseline Clearance of anti-tumor necrosis factors and human leukocyte antigen variant (HLA DQA1*05) in combination as poor prognostic factors (PPF) of pharmacokinetic (PK) origin impacting immune response (formation of antidrug antibodies) and disease control of inflammatory bowel disease (IBD) patients treated with infliximab or adalimumab.MethodsBaseline Clearance was estimated in IBD patients before starting treatment using weight and serum albumin concentrations. HLA DQA1*05 carrier status (rs2097432 A/G or G/G variant) was measured using real time polymerase chain reaction. The outcomes consisted of immune response, clinical and biochemical remission (C-reactive protein&lt;3 mg/L in the absence of symptoms), and endoscopic remission (SES-CD&lt;3). Statistical analysis consisted of logistic regression and nonlinear mixed effect models.Results and discussionIn 415 patients enrolled from 4 different cohorts (median age 27 [IQR: 15-43] years, 46% females), Clearance&gt;0.326 L/day and HLA DQA1*05 carrier status were 2-fold more likely to have antidrug antibodies (OR=2.3, 95%CI: 1.7-3.4; p&lt;0.001, and OR=1.9, 95%CI: 1.4-2.8; p&lt;0.001, respectively). Overall, each incremental PPF of PK origin resulted in a 2-fold (OR=2.16, 95%CI: 1.7-2.7; p&lt;0.01) higher likelihood of antidrug antibody formation. The presence of both PPF of PK origin resulted in higher rates of antidrug antibodies (p&lt;0.01) and lower clinical and biochemical remission (p&lt;0.01). Each incremental increase in PPF of PK origin associated with lower likelihood of endoscopic remission (OR=0.4, 95%CI: 0.2-0.7; p&lt;0.001). Prior biologic experience heightened the negative impact of PPF of PK origin on clinical and biochemical remission (p&lt;0.01). Implementation of proactive therapeutic drug monitoring reduced it, particularly during maintenance and in the presence of higher drug concentrations (p&lt;0.001). We conclude that PPF of PK origin, including both higher Clearance and carriage of HLA DQA1*05, impact outcomes in patients with IBD.
Rapid and simultaneous multiepitope antigen-based detection of Enterococcus by microscale thermophoresis and immunomagnetic separation
Authors: Liu Yan , Wang Ziyan , Wang Ze , Zhou Jun , Han Jiaojiao , Lu Chenyang , Liu Bing , Yu Rongxian , Sun Xiaoling , Zhang Zhen , Wang Rixin , Su Xiurong 
Published: January 2024

BackgroundGenerally, enterococci bacteria cause nosocomial infections and are major indicators of bacterial contamination in marine bathing beach. However, a method for the rapid and simultaneous detection of multiple pathogenic enterococci has not been developed on account of the wide variety of pathogenic enterococci and their existence in complex matrices.MethodsImmunoinformatics tools were used to design a multi-epitope antigen for the detection of various pathogenic enterococci by using the sequence of dltD gene on enterococci lipoteichoic acid (LTA) surface, which is associated with toxicological effects. The multi-epitopes included enterococci such as Enterococcus faecalis, E. gallinarum, E. raffinosus, E. durans, E. faecium, E. hirae, E. thailandicus, E. casseliflavus, E. avium, E. mundtii, E. lactis, E. solitarius, E. pseudoavium, and E. malodoratum. Microscale thermophoresis (MST) and western blot were carried out to detect the affinity between multi-epitope antigens and antibodies and between multi-epitope antibodies and bacteria. Furthermore, the detection of pathogenic enterococci was carried out by using immunomagnetic beads (IMBs) and immune chromatographic test strip (ICTS).ResultsThe multi-epitope antibody had a satisfactory affinity to the antigen and enterococci. IMBs and ICTS were detected with a minimum of 101 CFU/mL and showed incompatibility for Vibrio parahemolyticus, V. vulnifcus, V. harveyi, V. anguillarum, and Edwardsiella tarda.ImplicationThe present study demonstrated that the multi-epitope antigens exhibited excellent specificity and sensitivity, making them highly suitable for efficient on-site screening of enterococci bacteria in marine bathing beaches.
Rapid discrimination between clinical Clostridioides difficile infection and colonization by quantitative detection of TcdB toxin using a real-time cell analysis system
Authors: Shen Yuhang , Lin Shan , You Peijun , Chen Yu , Luo Yun , Song Xiaojun , Chen Yunbo , Jin Dazhi 
Published: January 2024

ObjectivesIt is important to accurately discriminate between clinical Clostridioides difficile infection (CDI) and colonization (CDC) for effective antimicrobial treatment.MethodsIn this study, 37 stool samples were collected from 17 CDC and 20 CDI cases, and each sample were tested in parallel through the real-time cell analysis (RTCA) system, real-time PCR assay (PCR), and enzyme-linked immunosorbent assay (ELISA).ResultsRTCA-measured functional and toxical C. difficile toxin B (TcdB) concentrations in the CDI group (302.58 ± 119.15 ng/mL) were significantly higher than those in the CDC group (18.15 ± 11.81 ng/mL) (p = 0.0008). Conversely, ELISA results revealed no significant disparities in TcdB concentrations between the CDC (26.21 ± 3.57 ng/mL) and the CDI group (17.07 ± 3.10 ng/mL) (p = 0.064). PCR results indicated no significant differences in tcdB gene copies between the CDC (774.54 ± 357.89 copies/μL) and the CDI group (4,667.69 ± 3,069.87 copies/μL) (p = 0.407). Additionally, the functional and toxical TcdB concentrations secreted from C. difficile isolates were measured by the RTCA. The results from the CDC (490.00 ± 133.29 ng/mL) and the CDI group (439.82 ± 114.66 ng/mL) showed no significant difference (p = 0.448). Notably, RTCA-measured functional and toxical TcdB concentration was significantly decreased when mixed with pooled CDC samples supernatant (p = 0.030).ConclusionThis study explored the novel application of the RTCA assay in effectively discerning clinical CDI from CDC cases.
Maternal diet and human milk composition: an updated systematic review
Authors: Petersohn Inga , Hellinga Anneke H. , van Lee Linde , Keukens Nicole , Bont Louis , Hettinga Kasper A. , Feskens Edith J. M. , Brouwer-Brolsma Elske M. 
Published: January 2024

ContextExclusive breastfeeding for 6 months after birth provides infants with the best start for life. A review by Bravi et al. summarized the importance of maternal diet as a determinant of human milk composition based on data up to 2015, but evidence on nutrient intake level was limited.ObjectiveWe updated the review by Bravi et al., critically assessed differences in study designs and sampling methods, and graphically visualized trends and associations.Data sourcesPubMed was systematically searched for articles published between January 2015 and March 2021.Data extractionArticle screening, selection, and data extraction was done by two independent researchers, including a risk of bias assessment based on 11 criteria. Articles were eligible when including: quantitative information, commonly used effect estimates, healthy mother-infant dyads.ResultsTwenty seven observational and five intervention studies were identified (n = 7,138) and combined with results of Bravi et al. Fatty acids were still the most studied human milk components in relation to maternal diet (n = 17 studies) with maternal fish intake being predominantly positively associated with milk ALA (r = 0.28–0.42), DHA (r = 0.24–0.46), and EPA (r = 0.25–0.28) content. PUFAs from diet were generally positively correlated with their concentrations in milk, while SFA intake was negatively associated with several fatty acids in milk. Studies on associations with maternal diet and milk carbohydrates, proteins, vitamins and minerals were limited in number and varied in methods and results.ConclusionThis updated review shows that evidence on the association between maternal diet and human milk fatty acids is rapidly increasing, but still diversified in methodology and results. Further studies, preferably intervention studies, assessing diet and milk carbohydrates, proteins, vitamins and minerals are needed to be able draw conclusions on the importance of maternal diet for human milk composition as a whole.
Study of amino acids absorption and gut microbiome on consumption of pea protein blended with enzymes-probiotics supplement
Authors: Rathi Abhijit , Gaonkar Tejal , Dhar Debojyoti , Kallapura Gopalakrishna , Jadhav Swati 
Published: January 2024

The current randomized, double-blind, crossover clinical trial was conducted to evaluate changes in the amino acid absorption and gut microbiota on consumption of pea protein supplemented with an enzymes-probiotics blend (Pepzyme Pro). A total of 15 healthy subjects were instructed to take test (pea protein + Pepzyme Pro) or placebo (pea protein + maltodextrin) for 15 days with a 30-day washout period. Blood samples were analyzed for plasma-free amino acids, insulin, and C-reactive protein (CRP). Additionally, nitrogen levels in urine and feces, along with the composition of gut microbiota, were evaluated. On day 15, the test arm showed a tendency to increase the rate of absorption and total absorption (AUC) of amino acids compared with the placebo arm, though the increase was statistically insignificant. In addition, 15-day test supplementation showed a tendency to reduce Tmax of all the amino acids (statistically insignificant except alanine, p = 0.021 and glycine, p = 0.023) in comparison with the placebo supplementation. There were no changes in urine and fecal nitrogen levels as well as serum CRP levels in the test and placebo arm. The increase in serum insulin level after 4 h was statistically significant in both arms, whereas the insulin level of the placebo and test arm at 4 h was not statistically different. Supplementation showed changes with respect to Archaea and few uncharacterized species but did not show statistically significant variations in microbiome profile at the higher taxonomic levels. A study with large sample size and detailed gut microbiome analysis is warranted to confirm the results statistically as well as to characterize altered species. However, the current study could provide an inkling of a positive alteration in protein digestibility, amino acid absorption, and gut microbiome with regular consumption of protein and enzymes-probiotics blend.Clinical Trial Registration:clinicaltrials.gov/; identifier [CTRI/2021/10/037072].
Effectiveness and safety of the bevacizumab and erlotinib combination versus erlotinib alone in EGFR mutant metastatic non-small-cell lung cancer: systematic review and meta-analysis
Authors: Motta-Guerrero Rodrigo , Leon Garrido-Lecca Alejandro , Failoc-Rojas Virgilio E. , Calle-Villavicencio Ana , Villacorta-Carranza Robert , Huerta-Collado Yesenia , Torres-Mera Alicia , Valladares-Garrido Mario J. , Rivera-Francia Víctor , Carracedo Carlos , Raez Luis 
Published: January 2024

BackgroundThe EGFR gene encodes a protein that stimulates molecular pathways that allow the growth and development of the tumor microenvironment. The current preferred tyrosine kinase inhibitor (TKI) for the first-line treatment of EGFRm metastatic non-small cell lung cancer (NSCLC) is osimertinib. However, the combination of angiogenesis inhibitors and TKI has produced discordant results. We aimed to assess the effects of the bevacizumab and erlotinib combination in EGFRm metastatic NSCLC.MethodsUsing eligibility criteria focused on patients with EGFRm metastatic NSCLC treated with bevacizumab and erlotinib, we searched databases including clinical trial randomized studies and reviews published until April 15, 2023 in Medline (PubMed), Scopus, and Embase. Eight clinical trials (1,052 patients) were selected from 1,343 articles for quantitative and qualitative assessment. The risk of bias was assessed using the Cochrane Risk of Bias tool. Data were synthesized through random-effects meta-analysis.ResultsThe bevacizumab and erlotinib combination significantly improved the progression-free survival (PFS) (log(HR) = 0.63; 95% CI: 0.54–0.73, p &lt; 0.001) and overall response ratio (ORR) (RR = 0.79; 95% CI, 0.64–0.97, p = 0.03). However, it did not improve the overall survival (log(HR) = 0.93; 95% CI, 0.78–1.10, p = 0.38) and was associated with higher serious adverse events (SAEs) (OR = 3.48; 95% CI, 1.76–6.88, p = 0.005). A subgroup analysis suggested similar benefits in different mutation subtypes and brain metastasis condition. The evidence is limited by a moderate risk of bias across studies and heterogeneity in the reporting of SAEs.ConclusionsThe bevacizumab and erlotinib combination significantly improved PFS and ORR in EGFRm metastatic NSCLC but were also associated with higher-grade (≥3) adverse events. These results suggest that while the combination therapy may enhance progression-free survival and overall response, it does not improve the overall survival and is associated with higher toxicity. Thus, the treatment should be personalized based on individual patient comorbidities. Further prospective trials are needed to validate these results.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/#searchadvanced, identifier CDR 42022364692.