bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supporting early‐career dementia researchers: Identifying support needs and ways forward via a European study
Authors: Dupont Charlèss , Gilissen Joni , Dassen Fania C. M. , Branco Rita Maldonado , Heins Pascale , Heffernan Eithne , Bartels Sara Laureen 
Published: November 2023

AbstractINTRODUCTIONEarly‐career researchers contribute significantly to dementia research and clinical practice. However, a growing group of early‐career dementia researchers (ECDRs) lack appropriate support throughout their careers. Thus, we aim to (i) explore support needs, (ii) determine recommendations, and (iii) set the agenda for organizations to better support ECDRs.METHODSAn iterative, explanatory sequential mixed‐methods design was applied. First ECDRs' needs were identified using an online survey informed by the Vitae Researcher Development Framework. Next, priority areas were selected and explored qualitatively with ECDRs in two workshops, utilizing the World Café methodology.RESULTSSixty‐five ECDRs throughout Europe completed the survey, with the majority reporting that greater support is needed in terms of funding and career opportunities, social support and well‐being, and “wide‐reaching” dissemination.DISCUSSIONBased on the findings, six recommendations for support organizations, funding bodies, and universities to better support ECDRs are formulated, each intended for specific target audiences.Highlights
This article reports on focal points of career‐related support needed in doctoral education and postdoctoral employment to foster a healthier academic environment, including finance, work–life balance, dissemination of research findings, and supervision, both in general and in dementia fields specifically.
Funding and resources were identified as a significant challenge, and there was a call for more long‐term positions and transition funding for postdoctoral researchers.
Early‐career dementia researchers addressed the need for support in producing outputs for non‐academic audiences, including people living with dementia. The importance of disseminating research to diverse audiences has long been recognized; thus, it is critical that early‐career dementia researchers be supported in this effort.
Recommendations were formulated for researcher support (organizations), funding bodies, and universities. These recommendations include providing support for disseminating research to non‐academic audiences, offering training in supervision skills, and promoting peer‐to‐peer mentoring and social activities for early‐career dementia researchers.
Physical activity and brain amyloid beta: A longitudinal analysis of cognitively unimpaired older adults
Authors: Slee Michael G. , Rainey‐Smith Stephanie R. , Villemagne Victor L. , Doecke James D. , Sohrabi Hamid R. , Taddei Kevin , Ames David , Dore Vincent , Maruff Paul , Laws Simon M. , Masters Colin L. , Rowe Christopher C. , Martins Ralph N. , Erickson Kirk I. , Brown Belinda M. 
Published: November 2023

AbstractINTRODUCTIONThe current study evaluated the relationship between habitual physical activity (PA) levels and brain amyloid beta (Aβ) over 15 years in a cohort of cognitively unimpaired older adults.METHODSPA and Aβ measures were collected over multiple timepoints from 731 cognitively unimpaired older adults participating in the Australian Imaging, Biomarkers and Lifestyle (AIBL) Study of Aging. Regression modeling examined cross‐sectional and longitudinal relationships between PA and brain Aβ. Moderation analyses examined apolipoprotein E (APOE) ε4 carriage impact on the PA‐Aβ relationship.RESULTSPA was not associated with brain Aβ at baseline (β = –0.001, p = 0.72) or over time (β = –0.26, p = 0.24). APOE ε4 status did not moderate the PA‐Aβ relationship over time (β = 0.12, p = 0.73). Brain Aβ levels did not predict PA trajectory (β = –54.26, p = 0.59).DISCUSSIONOur study did not identify a relationship between habitual PA and brain Aβ levels.Highlights
Physical activity levels did not predict brain amyloid beta (Aβ) levels over time in cognitively unimpaired older adults (≥60 years of age).
Apolipoprotein E (APOE) ε4 carrier status did not moderate the physical activity–brain Aβ relationship over time.
Physical activity trajectories were not impacted by brain Aβ levels.
Virtual care delivery of whole family assessment and intervention with infants and preschoolers: a thematic analysis of clinician and family experiences
Authors: Philipp Diane A. , Szczech Klaudia , Hanson Nick L. , O'Hara Gabrielle , Puckett Janai 
Published: November 2023

AbstractIn this study, we explore family and clinician experiences with virtual care delivery of whole family assessment and therapy developed for infant and preschool‐aged children and adapted during the COVID‐19 pandemic. A clinical case study is also presented. Between September and November 2020, semi‐structured interviews were conducted with four clinicians working with families with children in the 0 to 5 population and with four caregivers with children aged 4–6 years (M = 5.3) involved in whole family assessment and intervention (i.e., Lausanne Trilogue Play [LTP]; Reflective Family Play [RFP]) at a community mental health facility. Clinicians represented various mental health disciplines. Qualitative data were analysed using inductive thematic analysis with intercoder reliability established. Analysis of interviews generated nine themes organised within two conceptual frameworks, accessibility and efficacy. Accessibility included the themes: (1) flexibility, (2) privacy, and (3) resources. Efficacy comprised: (4) effects of technology, (5) home environment, (6) feasibility of therapy tasks, (7) parent alliance, (8) clinician fatigue, and (9) overall evaluation. Home environment was further divided into three subthemes: (5.1) disruptions, (5.2) boundaries, and (5.3) naturalistic observation. While participants reported benefits and challenges uniquely related to virtual care, both caregivers and clinicians expressed overall satisfaction with virtual whole family assessment and therapy. This study provides a rich exploration of the perspectives of caregivers and clinicians engaged in virtual whole family mental health care during the COVID‐19 pandemic. With adequate technology and privacy, whole family assessment and therapy, such as the LTP and RFP, provided via video teleconferencing facilitated accessible and effective care for families of young children with moderate to severe mental health challenges. Evidence suggests in‐person and hybrid approaches to whole family assessment and therapy could be further tailored to meet the needs of families with young children and infants.
The Lausanne Trilogue Play: bringing together developmental and systemic perspectives in clinical settings
Authors: Tissot Hervé , Favez Nicolas 
Published: November 2023

AbstractDevelopmentalists have demonstrated that the quality of relationships established by infants with their proximal social environment is crucial for lifecourse development. However, studies of parent–infant relationships have mostly centred on the mother–child dyad. Stemming from family systems theory that considers interactions within the whole family as critical for an individual's personal development, a group of family therapists and researchers in Lausanne (Switzerland) tried to bridge the gap between systemic and developmental thinking by stressing the need to establish the mother–father–infant triad as a collective unit of study. In response, they created the Lausanne Trilogue Play (LTP), a method to systematically assess the quality of mother–father–infant interactions. The LTP is an observational situation during which parents are asked to play with their infant in four parts: (i) one parent plays with the infant, while the other parent is ‘simply present’; (ii) the parents switch roles; (iii) all three play together; (iv) the parents discuss in front of the infant. The theoretical model underpinning the LTP is the family alliance model, which postulates that the quality of the coordination demonstrated by the triad to achieve this task can be assessed mainly through careful observation of non‐verbal behaviours as indicators of the achievement of four interactive functions (i.e., participation, organisation, focalisation, affect sharing); fulfilment of these functions determines the quality of relational functioning within the system. This article introduces the clinical, theoretical, and empirical foundations of using the LTP method with the family alliance model; its use in clinical and research contexts; and the most recent advances in the field of research on mother–father–infant interactions based on the LTP situation.
19th Asian Pacific Confederation of Chemical Engineering (APCChE) Congress 2022: Working towards a sustainable society—Special issue
Authors: Lee Tin Sin , Chong Chien Hwa 
Published: November 2023

AbstractThis special issue captures the papers of 19th Asian Pacific Confederation of Chemical Engineering (APCChE) Congress 2022 with the theme “Working Towards a Sustainable Society.” Since the announcement of 17 Sustainability Development Goals by United Nations, chemical engineers are obligated to use their expertise to engineer the industries towards environmental friendly technology. Chemical engineers are keen to contribute explicitly on energy conservation, water resources management, waste minimization, waste management, responsible consumption, recycling technology development, bioresources, pollution control as well as circular economy, education, and so on. These contributions primarily aim to enhance the quality of life, foster a healthier environment, and strengthen our community, ultimately improving prospects for the future.
Acrylic thiazole copolymer microspheres with “cluster effect” for long‐lasting antimicrobial activity and plant growth promotion
Authors: Zhang Yaping , Zhu Xinhua , Geng Yuanze , He Yufeng , Wang Rongmin 
Published: November 2023

AbstractThe environmentally friendly, low toxic or nontoxic, and functional material to solve the needs of agricultural development are the key to current scientific research. Here, acrylic thiazole copolymer clusters (AcTPCs) were successfully synthesized by using common methacrylic acid as an induced source, combined with the antimicrobial monomers acrylic thiazole (AcT) and Zn2+, which are of great value for agricultural applications. Utilizing the “cluster effect,” under the synergistic effect of the fully exposed active ingredients, they not only have long‐lasting antimicrobial effects against bacteria and fungi, but also promote the growth of plant roots and stems and increase the chlorophyll content. Therefore, this study provides a novel, environmental‐friendly, and low‐toxic method to solve the microbe infection problem, which is expected to improve the agriculture.
Encapsulation of anthocyanin with gelatin‐alginate carrier by a specially designed ultrasonic encapsulator with adjustable frequency
Authors: Sarıoğlu Kemal , Ülgen Ahmet , Oral Rasim Alper 
Published: November 2023

AbstractAnthocyanins are of increasing interest for food applications due to their health benefits. However, their poor stability and susceptibility to unfavorable environmental conditions may limit their application. Microencapsulation offers enhanced stability and controlled release properties. The aim was designing an ultrasonic encapsulator with a variable frequency ultrasonic driver to encapsulate black carrot and mahaleb anthocyanins. First, a double‐channel variable frequency and power adjustable ultrasonic driver and atomizer head were designed. The cooled gelation technique was used to encapsulate anthocyanin in an alginate‐gelatin complex. Freeze‐drying and air‐drying techniques were used for drying of microcapsules. Encapsulation parameters were atomization frequency, alginate, and anthocyanin concentrations. The capsules were characterized by encapsulation efficiency, particle diameter, and controlled release rates. Controlled release tests were performed at two different temperatures and pH values. Encapsulation efficiency was 87%–95%, mean diameters were between 69 and 95 μm. Maximum release rates were obtained from freeze‐dried mahaleb anthocyanin capsules. Release rates increased with increasing temperature and pH values. In addition, the experimental results showed that the increase in the amount of alginate used in encapsulation decreased the release rate. Moreover, the results proved that the designed encapsulator can be used for encapsulation in fields such as food, pharmaceuticals, polymers, chemistry, and materials.
Palladium‐Catalyzed Tandem Cyclization of Bromoalkynes, Anilines and CO: Access to 1,3‐Substituted Maleimides
Authors: Zeng Caijin , Su Shaoting , Fang Songjia , Jiang Huangfeng , Yang Shaorong , Wu Wanqing 
Published: November 2023

AbstractA novel palladium‐catalyzed three‐component carbonylation reaction for the assembly of various 1,3‐substituted maleimide derivatives from haloalkynes and simple anilines. The nucleophilic addition reaction of haloalkynes, anilines and CO, and insertion of carbonyl have been achieved sequentially in this reaction. The high chemo‐ and regioselectivities, as well as no need of expensive ligands or additives further illustrate the synthetic value of this approach.
Drug combinations screening using a Bayesian ranking approach based on dose–response models
Authors: Boumendil Luana , Fontaine Morgane , Lévy Vincent , Pacchiardi Kim , Itzykson Raphaël , Biard Lucie 
Published: November 2023

AbstractDrug combinations have been of increasing interest in recent years for the treatment of complex diseases such as cancer, as they could reduce the risk of drug resistance. Moreover, in oncology, combining drugs may allow tackling tumor heterogeneity. Identifying potent combinations can be an arduous task since exploring the full dose–response matrix of candidate combinations over a large number of drugs is costly and sometimes unfeasible, as the quantity of available biological material is limited and may vary across patients. Our objective was to develop a rank‐based screening approach for drug combinations in the setting of limited biological resources. A hierarchical Bayesian 4‐parameter log‐logistic (4PLL) model was used to estimate dose–response curves of dose–candidate combinations based on a parsimonious experimental design. We computed various activity ranking metrics, such as the area under the dose–response curve and Bliss synergy score, and we used the posterior distributions of ranks and the surface under the cumulative ranking curve to obtain a comprehensive final ranking of combinations. Based on simulations, our proposed method achieved good operating characteristics to identifying the most promising treatments in various scenarios with limited sample sizes and interpatient variability. We illustrate the proposed approach on real data from a combination screening experiment in acute myeloid leukemia.
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data
Authors: Gröner Albrecht 
Published: November 2023

AbstractThe capacity to remove viruses by Planova filters produced by Asahi Kasei, primarily by small virus‐retentive filters, were compiled from data in peer‐reviewed publications and, partly, publicly available data from presentations at conferences (Planova workshops). Data from more than 100 publications and presentations at conferences covering Planova filters were assessed. The data were grouped according to the different virus filters regarding mean pore sizes and viruses of different sizes for plasma and cell culture derived products. Planova 15N and 20N filters removed parvoviruses below the limit of detection of viruses in the filtrate in approx. 50% of all studies and mean LRFs (log reduction factors) for viruses detected in the filtrate were above 4, demonstrating effective parvovirus reduction. Parvovirus removal capacity increased for Planova BioEX filters as well as for 2 Planova 20N in series. Large viruses as retroviruses (e.g., HIV and MuLV), herpesviruses, flaviviruses and togaviruses were removed effectively by Planova 15N, 20N and BioEX filters and also by Planova 35N filters. Flow interruption, transmembrane pressure, volume and protein concentration per filter area had had no substantial impact on virus removal capacity at manufacturing specification. In conclusion, the incorporation of Planova filters in manufacturing processes of biologicals remove, depending on the filter pore size, small and large viruses from the feed stream reliably. This virus reduction step with an orthogonal mechanism integrated in the manufacturing processes of biologicals, based primarily on size exclusion of viruses, improves the virus safety of these biopharmaceutical products considerably.
Understanding adsorption behavior of Polysorbate‐20 to sterile filters in therapeutic proteins final filtration process
Authors: Ren Jian , Zhang Yun , Wang Chen 
Published: November 2023

AbstractSurfactants are commonly used in the therapeutic protein manufacturing process as stabilizer. Polysorbate‐20 (PS‐20) is one of the most commonly used surfactants to mitigate protein aggregation in the therapeutic protein formulation. It has been observed that polysorbate can be adsorbed by sterile filters during the final filtration process, which poses risk of uneven distribution and potentially reduced polysorbate concentration in final products. In this study, we evaluated the PS‐20 adsorption behavior using commonly used sterilizing‐grade microfiltration (MF) membranes via a customized filtration set‐up. The effect of membrane properties, including the membrane materials, modification, and layer configuration were studied. In addition, the effect of PS‐20 concentration was evaluated and the PS‐20 adsorption amount by each type of membranes was determined quantitatively and showed good agreement with the in‐process adsorption results. Finally, the selection of sterile filters and strategy of pre‐flush are discussed for ensuring accurate PS‐20 content in the final therapeutic protein drug product.
Impact of interstitial flow on cartilage matrix synthesis and NF‐kB transcription factor mRNA expression in a novel perfusion bioreactor
Authors: Abusharkh Haneen A. , Robertson Terreill , Mendenhall Juana , Gozen Bulent A. , Tingstad Edwin M. , Abu‐Lail Nehal I. , Thiessen David B. , Van Wie Bernard J. 
Published: November 2023

AbstractThis work is focused on designing an easy‐to‐use novel perfusion system for articular cartilage (AC) tissue engineering and using it to elucidate the mechanism by which interstitial shear upregulates matrix synthesis by articular chondrocytes (AChs). Porous chitosan‐agarose (CHAG) scaffolds were synthesized and compared to bulk agarose (AG) scaffolds. Both scaffolds were seeded with osteoarthritic human AChs and cultured in a novel perfusion system with a medium flow velocity of 0.33 mm/s corresponding to 0.4 mPa surfice shear and 40 mPa CHAG interstitial shear. While there were no statistical differences in cell viability for perfusion versus static cultures for either scaffold type, CHAG scaffolds exhibited a 3.3‐fold higher (p < 0.005) cell viability compared to AG scaffold cultures. Effects of combined superficial and interstitial perfusion for CHAG showed 150‐ and 45‐fold (p < 0.0001) increases in total collagen (COL) and 13‐ and 2.2‐fold (p < 0.001) increases in glycosaminoglycans (GAGs) over AG non‐perfusion and perfusion cultures, respectively, and a 1.5‐fold and 3.6‐fold (p < 0.005) increase over non‐perfusion CHAG cultures. Contrasting CHAG perfusion and static cultures, chondrogenic gene comparisons showed a 3.5‐fold increase in collagen type II/type I (COL2A1/COL1A1) mRNA ratio (p < 0.05), and a 1.3‐fold increase in aggrecan mRNA. Observed effects are linked to NF‐κB signal transduction pathway inhibition as confirmed by a 3.2‐fold (p < 0.05) reduction of NF‐κB mRNA expression upon exposure to perfusion. Our results demonstrate that pores play a critical role in improving cell viability and that interstitial flow caused by medium perfusion through the porous scaffolds enhances the expression of chondrogenic genes and extracellular matrix through downregulating NF‐κB1.
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation
Authors: Pan Duo‐Tao , Wang Pan , Wang Xiao‐Li , Sun Ya‐Qin , Xiu Zhi‐Long 
Published: November 2023

AbstractTo study the relationship between the yield of 1,3‐propanediol (1,3‐PDO) and the flux change of the Clostridium butyricum metabolic pathway, an optimized calculation method based on dynamic flux balance analysis was used by combining genome‐scale flux balance analysis with a kinetic model. A more comprehensive and extensive metabolic pathway was obtained by optimization calculations. The primary extended branches include: the dihydroxyacetone node, which enters the pentose phosphate pathway; the α‐oxoglutarate node, which has synthetic metabolic pathways for glutamic acid and amino acids; and the serine and homocysteine nodes, which produce cystathionine before homocysteine enters the methionine cycle pathway. According to the expanded metabolic network, the flux distribution of key nodes in the metabolic pathway and the relationship between the flux distribution ratio of nodes and the yield of 1,3‐PDO were analyzed. At the dihydroxyacetone node, the flux of dihydroxyacetone converted to dihydroxyacetone phosphate was positively correlated with the yield of 1,3‐PDO. As an important intermediate product, the flux change in the metabolic pathway of α‐oxoglutarate reacting with amino acids to produce glutamic acid is positively correlated with the yield. When pyruvate was used as the central node to convert into lactic acid and α‐oxoglutarate, the proportion of branch flux was negatively correlated with the yield of 1,3‐PDO. These studies provide a theoretical basis for the optimization and further study of the metabolic pathway of C. butyricum.
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers
Authors: de Siqueira Edmilson Clarindo , de Andrade Alves Aline , da Costa e Silva Páblo Eugênio , de Barros Maria Paloma Silva , Houllou Laureen Michelle 
Published: November 2023

AbstractPolyhydroxyalkanoates (PHAs) and exopolysaccharides (EPSs) belong to a class of abundant biopolymers produced by various fermenting microorganisms. These biocompounds have high value‐added potential and can be produced concurrently. Co‐production of PHAs and EPSs is a strategy employed by researchers to reduce costs associated with large‐scale production. EPSs and PHAs are non‐toxic, biocompatible, and biodegradable, making them suitable for various industrial sectors, including packaging and the medical and pharmaceutical industries. These biopolymers can be derived from agro‐industrial residues, thus contributing to the bioeconomy by producing high‐value‐added products. This review investigates approaches for simultaneously synthesizing PHAs and EPSs using different carbon sources and microorganisms.