bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intersection of Immunity, Metabolism, and Muscle Regeneration in an Autoimmune‐Prone MRL Mouse Model
Authors: Ngo Tran B. , Josyula Aditya , DeStefano Sabrina , Fertil Daphna , Faust Mondreakest , Lokwani Ravi , Sadtler Kaitlyn 
Published: January 2024

AbstractDue to the limited capacity of mammals to regenerate complex tissues, researchers have worked to understand the mechanisms of tissue regeneration in organisms that maintain that capacity. One example is the MRL/MpJ mouse strain with unique regenerative capacity in ear pinnae that is absent from other strains, such as the common C57BL/6 strain. The MRL/MpJ mouse has also been associated with an autoimmune phenotype even in the absence of the mutant Fas gene described in its parent strain MRL/lpr. Due to these findings, the differences between the responses of MRL/MpJ versus C57BL/6 strain are evaluated in volumetric muscle injury and subsequent material implantation. One salient feature of the MRL/MpJ response to injury is robust adipogenesis within the muscle. This is associated with a decrease in M2‐like polarization in response to biologically derived extracellular matrix scaffolds. In pro‐fibrotic materials, such as polyethylene, there are fewer foreign body giant cells in the MRL/MpJ mice. As there are reports of both positive and negative influences of adipose tissue and adipogenesis on wound healing, this model can provide an important lens to investigate the interplay between stem cells, adipose tissue, and immune responses in trauma and material implantation.
Controllable Assembly of Highly Oxidized Cobalt on Graphdiyne Surface for Efficient Conversion of Nitrogen into Nitric Acid
Authors: Zheng Xuchen , Wu Han , Gao Yang , Chen Siao , Xue Yurui , Li Yuliang 
Published: January 2024

AbstractThe manufacture of nitric acid (HNO3) consumes large amounts of energy and causes serious environmental pollution. Electrochemical synthesis is regarded as a key way to eliminate carbon emissions from the chemicals industry. The selective electrosynthesis of HNO3 from nitrogen was achieved by controllable assembly of cobalt metal on graphdiyne surface using a powerful tool of electrochemistry at ambient conditions. As an advanced material, graphdiyne (GDY) has a large conjugated structure on its surface and is rich in sp‐C triple bond skeleton, which can achieve strong interaction with metal atoms, resulting in incomplete charge transfer between graphdiyne and cobalt atoms. The experimental and theoretical calculation results show that the highly oxidized cobalt on graphdiyne (HOCo/GDY) can selectively and efficiently activate and convert the nitrogen into the key intermediate *NO, which promotes the efficient overall conversion performance of nitrogen to nitric acid. Thus, the highest nitric acid yield (192.0 μg h−1 mg−1) and Faradaic efficiency (21.5 %) were achieved at low potentials.
Metallo‐Supramolecular Helical Fibres from Chiral Phenylacetylene Monomers: Cation Induced Self‐Assembly
Authors: Rey‐Tarrío Francisco , Simón‐Fuente Silvia , Cuerva Juan M. , Miguel Delia , Ribagorda Maria , Quiñoá Emilio , Freire Félix 
Published: January 2024

AbstractChiral metallo‐supramolecular fibres can be easily obtained by mixing a chloroform solution of a phenylacetylene monomer (PA) that bears a chiral sulfoxide group as pendant, with different equivalents of a methanolic solution of AgClO4. Thus, while the PA is found molecularly dissolved in chloroform, the addition of Ag+ ions induce its aggregation through the formation of an axially chiral metallo‐supramolecular aggregate with high thermal stable properties. In this case, the ability of the metal ion to coordinate the PA triple bond, combined with the argentophilicity of the metal ion and the planarity of the phenylacetylene drives to the formation of a helical coordination polymer, whose P or M axial chirality is determined by the chirality of the sulfoxide used as substituent of the PA. Depending on the PA/Ag+ (mol/mol) ratio, it is possible to tune the morphology of the metallo‐supramolecular aggregate from chiral fibers to chiral gel.
L‐Glycosidase‐Cleavable Natural Glycans Facilitate the Chemical Synthesis of Correctly Folded Disulfide‐Bonded D‐Proteins
Authors: Shi Weiwei , Wang Tongyue , Yang Ziyi , Ren Yuxiang , Han Dongyang , Zheng Yupeng , Deng Xiangyu , Tang Shan , Zheng Ji‐Shen 
Published: January 2024

AbstractD‐peptide ligands can be screened for therapeutic potency and enzymatic stability using synthetic mirror‐image proteins (D‐proteins), but efficient acquisition of these D‐proteins can be hampered by the need to accomplish their in vitro folding, which often requires the formation of correctly linked disulfide bonds. Here, we report the finding that temporary installation of natural O‐linked‐β‐N‐acetyl‐D‐glucosamine (O‐GlcNAc) groups onto selected D‐serine or D‐threonine residues of the synthetic disulfide‐bonded D‐proteins can facilitate their folding in vitro, and that the natural glycosyl groups can be completely removed from the folded D‐proteins to afford the desired chirally inverted D‐protein targets using naturally occurring O‐GlcNAcase. This approach enabled the efficient chemical syntheses of several important but difficult‐to‐fold D‐proteins incorporating disulfide bonds including the mirror‐image tumor necrosis factor alpha (D‐TNFα) homotrimer and the mirror‐image receptor‐binding domain of the Omicron spike protein (D‐RBD). Our work establishes the use of O‐GlcNAc to facilitate D‐protein synthesis and folding and proves that D‐proteins bearing O‐GlcNAc can be good substrates for naturally occurring O‐GlcNAcase.
Photo‐Controlled Calcium Overload from Endogenous Sources for Tumor Therapy
Authors: Hu Jing‐Jing , Yuan Lizhen , Zhang Yunfan , Kuang Jing , Song Wen , Lou Xiaoding , Xia Fan , Yoon Juyoung 
Published: January 2024

AbstractDesigning reactive calcium‐based nanogenerators to produce excess calcium ions (Ca2+) in tumor cells is an attractive tumor treatment method. However, nanogenerators that introduce exogenous Ca2+ are either overactive incapable of on‐demand release, or excessively inert incapable of an overload of calcium rapidly. Herein, inspired by inherently diverse Ca2+‐regulating channels, a photo‐controlled Ca2+ nanomodulator that fully utilizes endogenous Ca2+ from dual sources was designed to achieve Ca2+ overload in tumor cells. Specifically, mesoporous silica nanoparticles were used to co‐load bifunctional indocyanine green as a photodynamic/photothermal agent and a thermal‐sensitive nitric oxide (NO) donor (BNN‐6). Thereafter, they were coated with hyaluronic acid, which served as a tumor cell‐targeting unit and a gatekeeper. Under near‐infrared light irradiation, the Ca2+ nanomodulator can generate reactive oxygen species that stimulate the transient receptor potential ankyrin subtype 1 channel to realize Ca2+ influx from extracellular environments. Simultaneously, the converted heat can induce BNN‐6 decomposition to generate NO, which would open the ryanodine receptor channel in the endoplasmic reticulum and allow stored Ca2+ to leak. Both in vitro and in vivo experiments demonstrated that the combination of photo‐controlled Ca2+ influx and release could enable Ca2+ overload in the cytoplasm and efficiently inhibit tumor growth.
Impact of single and mixed cultures of yeasts and lactic acid bacteria in coffee fermentation and cup quality
Authors: Estela‐Escalante Waldir D. , Rodriguez‐Portilla Liz M. I. , Pinillos‐Miñano Ricardo M. , Rojas‐Ocupa Elton J. , Lozada‐Castillo Karina L. , Hatta‐Sakoda Beatriz A. , Pérez Americo Guevara 
Published: January 2024

AbstractSaccharomyces cerevisiae LBTF 21.3, Pichia manshurica LBTF 21.1, and Leuconostoc lactis LBTF 21.1 were used as single and mixed cultures in coffee fermentations. Fermentations carried out in glass jars with P. manshurica LBTF 21.1/Leuc. lactis LBTF 21.1 showed higher fermentation rates than S. cerevisiae LBTF 21.3/P. manshurica LBTF 21.1 or S. cerevisiae LBTF 21.3/Leuc. lactis LBTF 21.1. The highest cup quality was reached with P. manshurica LBTF 21.1/Leuc. lactis LBTF 21.1 (83.0 points). Full factorial design (22) carried out with P. manshurica LBTF 21.1 revealed that the higher proportion of water added before the fermentation resulted in higher production of acetic acid. A contrary effect of this variable was observed on the production of lactic acid with Leuc. lactis LBTF 21.1. Fermentations conducted in bioreactor evidenced the production of acetaldehyde by P. manshurica LBTF 21.1/Leuc. lactis LBTF 21.1, P. manshurica LBTF 21.1, and Leuc. lactis LBTF 21.1. Additionally, the highest cup quality was reached in fermentations with P. manshurica LBTF 21.1 (82.5 points) followed by Leuc. lactis LBTF 21.1 (81.5 points) and P. manshurica LBTF 21.1/Leuc. lactis LBTF 21.1 (81.0 points). Fermentations of coffee with single and mixed cultures are interesting strategies for the improvement of the sensory quality.
Good new(t)s: Rapid recolonization of a restored fish‐invaded habitat by two newt species in southern Italy
Authors: Chiacchio Michele , Paudice Giuseppe , Senese Andrea , Russo Valerio Giovanni 
Published: January 2024

Abstract

Habitat modification and invasive species are considered some of the most impelling causes of the ongoing sixth mass extinction, especially in freshwater ecosystems. Artificial water troughs are, in this regard, particularly vulnerable environments, while being pivotal breeding habitats for many amphibian species. However, despite their well‐known importance and their conservation concern, detailed information on the resilience of such ecosystems is lacking.
This note describes the events following the introduction of invasive fish species into a water trough in southern Italy and reconstructs the recolonization times of two newt species (Lissotriton italicus, Triturus carnifex) after disappearance of the fish a few months later.
As expected, both newt species were no longer observed in the water as soon as the fish were introduced in the trough. However, in contrast to previous reports, they recolonized it within 2 months of the last observation of fish and, in the case of L. italicus, bred shortly after.
Although the short permanence of fish in the trough may have facilitated the recolonization process, this event represents the fastest recolonization by newts of a restored habitat ever reported in the wild and sheds further insights on the resilience of amphibian species.
In vitro protein protection of protein meals using Bioprotect and tannin extract from red grape marc
Authors: Tunkala Bereket Zeleke , DiGiacomo Kristy , Alvarez Hess Pablo S. , Dunshea Frank R. , Leury Brian J. 
Published: January 2024

AbstractProtecting crude protein in the rumen may reduce extensive protein degradation and ammonia emission and increase available bypass protein in ruminants. This experiment was conducted to determine the effect of two Bioprotect (15 and 30 mL/kg dry matter (DM)) and two tannin extract (TE) (20 and 40 g/kg DM) inclusion rates on protein protection and in vitro fermentation characteristics of canola and soybean meals incubated for 24 h using an ANKOM in vitro gas production system. The treated canola and soybean meals produced lower soluble protein (fraction ‘a’) and larger slowly degradable protein (fraction ‘b’) than its untreated counterparts, p < 0.01. However, the 20 g/kg DM TE inclusion showed lowest effect on the amount of protein fractions ‘a’ and ‘b’ in both meals compared to their other treated counterparts. The increasing concentration of additives reduced the total volatile fatty acids (VFA), p < 0.001. The effects of additives differed between the treatments as 15 mL/kg DM Bioprotect and 20 g/kg DM TE did not affect the acetic to propionic acid ratio (A:P) and the time before gas production began. The increase in fraction ‘b’ and reduction in protein fraction ‘a’ confirm successful protein protection in this experiment. However, the extensive reduction in ammonia‐N and in vitro degradable protein after using 30 mL/kg DM Bioprotect suggests possible toxicity to the microbes responsible for protein digestion in higher doses. Therefore, 15 mL/kg DM Bioprotect and 40 g/kg DM TE could be promising protein protection doses for in vitro experiments.
Light vector mesons photoproduction and nuclear shadowing
Authors: Trebien Haimon 
Published: January 2024

AbstractIn this work, we study the photoproduction of mesons considering the proton and the nucleus as the target. Utilizing the dipole picture and the wave functions obtained via AdS/QCD, we were able to describe the HERA data and extend the formalism to the nuclear case considering the Glauber–Gribov model. The preliminary results obtained in the nuclear regime are compared to the recent LHC data and suggest the presence of a nuclear effect called shadowing.
Correlation between essential and toxic elements in maternal blood during early pregnancy and atrial septal defects/ventricular septal defects/patent ductus arteriosus in offspring
Authors: Gu Jianli , Jin Zhen , Wu Guiqing , Dang Shaonong , Yao Feng , Zheng Zihan , Ren Lingxuan , Yang Jianjun , Chen Danli , Zhang Li , Lin Rong 
Published: January 2024

AbstractBackgroundCongenital heart defects (CHDs) are the most common congenital malformation in the world. Recent studies have found that essential and toxic trace element levels may play a crucial role in the risk of neonatal malformation. However, the relationships between element levels in early pregnancy and CHD risk among humans remain unclear. This study investigates the association between maternal essential element (copper [Cu], zinc [Zn], calcium [Ca], manganese [Mg] and iron [Fe]) and toxic element (lead [Pb] and cadmium [Cd]) levels during early pregnancy and CHDs.MethodsA hospital‐based case–control study was conducted, including 181 cases and 218 controls. Eligible participants underwent antenatal examination during gestational weeks 11–14 and trace element levels were detected by the atomic absorption method. Multi‐variable logistic regression was used to examine the associations between the level of maternal trace elements and CHD risks.ResultsHigher levels of Ca in early pregnancy were associated with lower risk of ASD/VSD risks. Moreover, higher Fe, Pb, and Cd levels in the first trimester were associated with higher risks of all CHD and the subtypes risks, and the tests for trend were significant (all p < .05). The restricted cubic spline analysis showed that there was a nonlinear inverted u‐shaped dose–response relationship between levels of Zn, Pb, and Cd in the first trimester and risk of CHDs (non‐linearity test p < .05).ConclusionsA moderate increase in Zn and Ca levels and a decrease in Pb and Cd levels during early pregnancy are needed to reduce the incidence of CHDs in the Chinese population.
Learning from failure: A context‐informed perspective on RCTs
Authors: Coldwell Mike , Moore Nick 
Published: January 2024

AbstractDiscussions of randomised controlled trials (RCTs) in education that do not show an impact regularly focus on the intervention and how it failed to impact on expected measures, with typologies identifying persistent critical points of failure. This paper uses one such RCT—the Integrating English programme—to exemplify the application of a new model to explain failure in RCTs. To do so, the paper develops a set of categories of context drawing on the wider social evaluation field: backdrop, design, operation and interpretation. Thus, the paper exposes critical weak points in the commission and interpretation, as well as the implementation, of an RCT. Our aim is to work towards more robust evaluations by demonstrating that it is not simply the programme design, implementation and evaluation that can contribute to a lack of impact; there can be more fundamental system issues at play.
Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats
Authors: Miler Marko , Živanović Jasmina , Ajdžanović Vladimir , Milenkovic Dragan , Cesar Thais , Filipović Miloš R. , Milošević Verica 
Published: January 2024

AbstractCitrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24‐month‐old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for 4 weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total ‐SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate–cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old‐aged liver.
Novel Benzotriazole‐β‐lactam Derivatives as Antimalarial Agents: Design, Synthesis, Biological Evaluation and Molecular Docking Studies
Authors: Aye Malihe , Jarrahpour Aliasghar , Haghighijoo Zahra , Heiran Roghayeh , Pournejati Roya , Karbalaei‐Heidari Hamid Reza , Sinou Veronique , Brunel Jean Michel , Akkurt Mehmet , Özdemir Namık , Turos Edward 
Published: January 2024

AbstractMany people around the world suffer from malaria, especially in tropical or subtropical regions. While malaria medications have shown success in treating malaria, there is still a problem with resistance to these drugs. Herein, we designed and synthesized some structurally novel benzotriazole‐β‐lactams using 2‐(1H‐benzo[d][1,2,3]triazol‐1‐yl)acetic acid as a key intermediate. To synthesize the target molecules, the ketene‐imine cycloaddition reaction was employed. First, The reaction of 1H‐benzo[d][1,2,3]triazole with 2‐bromoacetic acid in aqueous sodium hydroxide yielded 2‐(1H‐benzo[d][1,2,3]triazol‐1‐yl)acetic acid. Then, the treatment of 2‐(1H‐benzo[d][1,2,3]triazol‐1‐yl)acetic acid with tosyl chloride, triethyl amine, and Schiff base provided new β‐lactams in good to moderate yields.The formation of all cycloadducts was confirmed by elemental analysis, FT‐IR, NMR and mass spectral data. Moreover, X‐ray crystallography was used to determine the relative stereochemistry of 4a compound. The in vitro antimalarial activity test was conducted for each compound against P. falciparum K1. The IC50 values ranged from 5.56 to 25.65 μM. A cytotoxicity profile of the compounds at 200 μM final concentration revealed suitable selectivity of the compounds for malaria treatment. Furthermore, the docking study was carried out for each compound into the P. falciparum dihydrofolate reductase enzyme (PfDHFR) binding site to analyze their possible binding orientation in the active site.
Cyanide poisoning after bitter almond ingestion: “A rare case report”
Authors: Arabizadeh Neda , Mahmoudi Masoud , Gandomani Laaya Mokhtar , Eizadi‐Mood Nastaran 
Published: January 2024

Key Clinical MessageWe present a case of a 36‐year‐old woman with a history of three suicide attempts who had ingested approximately 40 bitter almonds in a suicidal act, leading to her admission to the emergency department of a regional hospital due to complaints of vomiting. Upon arrival, she exhibited confusion, and her vital signs were recorded as follows: pulse rate = 117 beats/min, blood pressure = 160/85 mmHg, oxygen saturation = 95%, respiratory rate = 16, temperature = 37°C. The patient venous blood gas analysis manifested severe metabolic acidosis (pH = 6.92, pO2 = 43 mmHg, HCO3 = 8.6 mmol/L, pCO2 = 42.7 mmHg, base excess = −25.9 mmol/L). Four hours later, she became unconscious and she was intubated. Gastric lavage and a single dose of 60 g of activated charcoal and sodium bicarbonate were administered. In the referral hospital, sodium nitrite was given due to the severity of the poisoning, and norepinephrine infusion was initiated to manage hypotension. Within a day, the patient regained consciousness, underwent extubation, and after 72 h was discharged and subsequently transferred to psychiatric care for further treatment. This case underscores the critical, life‐threatening implications of cyanide toxicity following the ingestion of bitter almonds, highlighting the efficacy of supportive measures such as gastric lavage, activated charcoal, and sodium bicarbonate. Furthermore, it emphasizes the successful application of sodium nitrite monotherapy in managing this condition.
Enhanced luminescence of a silica‐nitride phosphor; La3Si6N11:Ce3+by using Cr3+‐activated TiO2 nanoparticles
Authors: Aydin Ilkyaz , Ertekin Kadriye , Azem Funda Ak , Delice Tulay Koc , Birlik Isil 
Published: January 2024

AbstractCe3+‐activated silica nitride phosphors (lanthanum silicate‐nitride [LSN]) are promising thermostable conversion components for white‐light‐emitting diodes, screen technologies, and other optoelectronic applications. Cr3+‐activated TiO2, on the other hand, is a strongly absorbing material depending on its size and optical microenvironment. In this work, we gathered Cr3+‐doped TiO2 and La3Si6N11:Ce3+ in an optically transparent polymethylmethacrylate matrix. Photoluminescence of the binary blends of La3Si6N11:Ce3+/TiO2 and La3Si6N11:Ce3+/TiO2:Cr3+ has been investigated under two different excitation energies by steady‐state and lifetime‐based measurements, respectively. When the microscale La3Si6N11:Ce3+ phosphor and nanoscale TiO2:Cr3+ particles were gathered at a critical concentration, the resulting composite exhibited 3.14‐fold enhancement in the emission signal intensity with respect to the additive‐free phosphor. Upon excitation, the La3Si6N11:Ce3+–TiO2:Cr3+ blend exhibited different excited‐state lifetimes for the nano‐ and microsecond time‐scales compared to the LSN. The microsecond time‐scale measurements performed under 378 nm excitation supported the existence of a potential energy transfer from the TiO2:Cr3+ to the La3Si6N11:Ce3+.
Enhancing the Features of DNA Mimic Foldamers for Structural Investigations
Authors: Corvaglia Valentina , Wu Jiaojiao , Deepak Deepak , Loos Manuel , Huc Ivan 
Published: January 2024

AbstractDNA mimic foldamers based on aromatic oligoamide helices bearing anionic phosphonate side chains have been shown to bind to DNA‐binding proteins sometimes orders of magnitude better than DNA itself. Here, we introduce new features in the DNA mimic foldamers to facilitate structural investigations of their interactions with proteins. Thirteen new foldamer sequences have been synthesized and characterized using NMR, circular dichroism, molecular modeling, and X‐ray crystallography. The results show that foldamer helix handedness can be quantitatively biased by means of a single stereogenic center, that the foldamer structure can be made C2‐symmetrical as in palindromic B‐DNA sequences, and that associations between foldamer helices can be promoted utilizing dedicated C‐terminal residues that act as sticky ends in B‐DNA structures.