bluuebunny/crossref_metadata_2025_split huggingface dataset:
A quest for an integrated management system of children following a drowning incident: A review of the literature
Authors: Rossouw Seugnette , Maree Carin , Latour Jos M. 
Published: December 2023

AbstractPurposeManagement of children following a drowning incident is based on specific interventions which are used in the prehospital environment, the emergency department (ED) and the Paediatric Intensive Care Unit (PICU). This paper presents a review of the literature to map and describe the management and interventions used by healthcare professionals when managing a child following a drowning incident. Of specific interest was to map, synthesise and describe the management and interventions according to the different clinical domains or practice areas of healthcare professionals.Design and MethodsA traditional review of the literature was performed to appraise, map and describe information from 32 relevant articles. Four electronic databases were searched using search strings and the Boolean operators AND as well as OR. The included articles were all published in English between 2010 and 2022, as it comprised a timeline including current guidelines and practices necessary to describe management and interventions.ResultsConcepts and phrases from the literature were used as headings to form a picture or overview of the interventions used for managing a child following a drowning incident. Information extracted from the literature was mapped under management and interventions for prehospital, the ED and the PICU and a figure was constructed to display the findings. It was evident from the literature that management and interventions are well researched, evidence‐informed and discussed, but no clear arguments or examples could be found to link the interventions for integrated management from the scene of drowning through to the PICU. Cooling and/or rewarming techniques and approaches and termination of resuscitation were found to be discussed as interventions, but no evidence of integration from prehospital to the ED and beyond was found. The review also highlighted the absence of parental involvement in the management of children following a drowning incident.Practice ImplicationsMapping the literature enables visualisation of management and interventions used for children following a drowning incident. Integration of these interventions can collaboratively be done by involving the healthcare practitioners to form a link or chain for integrated management from the scene of drowning through to the PICU.
Household food security and dietary diversity in south‐eastern Nigeria
Authors: Ukonu Ijeoma C. , Wallace Carol A. , Lowe Nicola M. 
Published: December 2023

AbstractThe aim of this study was to investigate household food security (access) level and the dietary diversity of households in the Nsukka Local Government Area in South‐eastern Nigeria. From 20 local communities of Nsukka, 390 women were randomly sampled from the women's group and asked to complete a survey that determined the Household Food Insecurity Access Scale scores and the Household Dietary Diversity Scores (HDDS). The descriptive results indicated a high level of food insecurity with 82.6% households reporting various degrees of food insecurity. Over half of the sampled population experienced insufficient food quality. They either ate unwanted food (65.9%), limited variety (63.1%), or unpreferred food (64.6%). Some households experienced insufficient food intake by going a whole day without food (38.2%), go to sleep hungry (45.1%), or have no food of any kind (49%). The analysis of variance showed no significant difference (p = 0.428) in the food security level of households headed by males as compared with those headed by females. Approximately 53.6% of households fell at or below the average HDDS; males headed 48% of these households, while females headed 64%. The chi‐square test indicated factors associated with household food security including age, education, work status and income, whereas the gender of the household head, household size and marital status were not significantly associated. Public–private partnerships, nutrition orientation and food intervention programs could improve food security in this area.
The tuber‐specific StbHLH93 gene regulates proplastid‐to‐amyloplast development during stolon swelling in potato
Authors: Yang Rui , Sun Yuan , Zhu Xiaoling , Jiao Baozhen , Sun Sifan , Chen Yun , Li Lizhu , Wang Xue , Zeng Qian , Liang Qiqi , Huang Binquan 
Published: December 2023

Summary
In potato, stolon swelling is a complex and highly regulated process, and much more work is needed to fully understand the underlying mechanisms.
We identified a novel tuber‐specific basic helix–loop–helix (bHLH) transcription factor, StbHLH93, based on the high‐resolution transcriptome of potato tuber development.
StbHLH93 is predominantly expressed in the subapical and perimedullary region of the stolon and developing tubers. Knockdown of StbHLH93 significantly decreased tuber number and size, resulting from suppression of stolon swelling. Furthermore, we found that StbHLH93 directly binds to the plastid protein import system gene TIC56 promoter, activates its expression, and is involved in proplastid‐to‐amyloplast development during the stolon‐to‐tuber transition. Knockdown of the target TIC56 gene resulted in similarly problematic amyloplast biogenesis and tuberization.
Taken together, StbHLH93 functions in the differentiation of proplastids to regulate stolon swelling. This study highlights the critical role of proplastid‐to‐amyloplast interconversion during potato tuberization.
Dicer‐dependent heterochromatic small RNAs in the model diatom species Phaeodactylum tricornutum
Authors: Grypioti Emilia , Richard Hugues , Kryovrysanaki Nikoleta , Jaubert Marianne , Falciatore Angela , Verret Frédéric , Kalantidis Kriton 
Published: December 2023

Summary
Diatoms are eukaryotic microalgae responsible for nearly half of the marine productivity. RNA interference (RNAi) is a mechanism of regulation of gene expression mediated by small RNAs (sRNAs) processed by the endoribonuclease Dicer (DCR). To date, the mechanism and physiological role of RNAi in diatoms are unknown.
We mined diatom genomes and transcriptomes for key RNAi effectors and retraced their phylogenetic history. We generated DCR knockout lines in the model diatom species Phaeodactylum tricornutum and analyzed their mRNA and sRNA populations, repression‐associated histone marks, and acclimatory response to nitrogen starvation.
Diatoms presented a diversification of key RNAi effectors whose distribution across species suggests the presence of distinct RNAi pathways. P. tricornutum DCR was found to process 26–31‐nt‐long double‐stranded sRNAs originating mostly from transposons covered by repression‐associated epigenetic marks. In parallel, P. tricornutum DCR was necessary for the maintenance of the repression‐associated histone marks H3K9me2/3 and H3K27me3. Finally, PtDCR‐KO lines presented a compromised recovery post nitrogen starvation suggesting a role for P. tricornutum DCR in the acclimation to nutrient stress.
Our study characterized the molecular function of the single DCR homolog of P. tricornutum suggesting an association between RNAi and heterochromatin maintenance in this model diatom species.
The MsDHN1‐MsPIP2;1‐MsmMYB module orchestrates the trade‐off between growth and survival of alfalfa in response to drought stress
Authors: Lv Aimin , Su Liantai , Fan Nana , Wen Wuwu , Gao Li , Mo Xin , You Xiangkai , Zhou Peng , An Yuan 
Published: December 2023

SummaryDehydrins and aquaporins play crucial roles in plant growth and stress responses by acting as protector and controlling water transport across membranes, respectively. MsDHN1 (dehydrin) and MsPIP2;1 (aquaporin) were demonstrated to interact with a membrane‐anchored MYB protein, MsmMYB (as mMYB) in plasma membrane under normal condition. MsDHN1, MsPIP2;1 and MsDHN1‐MsPIP2;1 positively regulated alfalfa tolerance to water deficiency. Water deficiency caused phosphorylation of MsPIP2;1 at Ser 272, which led to release C terminus of mMYB (mMYBΔ83) from plasma membrane and translocate to nucleus, where C terminus of MsDHN1 interacted with mMYBΔ83, and promoted mMYBΔ83 transcriptional activity in response to water deficiency. Overexpression of mMYB and mMYBΔ83 down‐regulated the expression of MsCESA3, but up‐regulated MsCESA7 expression by directly binding to their promoters, and resulted in high drought tolerance in transgenic hairy roots. These results indicate that the MsDHN1‐MsPIP2;1‐MsMYB module serves as a key regulator in alfalfa against drought stress.
Phenotype and genotype characterization of Botrytis cinerea isolates from cut roses in Yunnan, China
Authors: Yuan Wenbin , Han Yanglin , Li Sisi , Ullah Ikram , He Shuilian , Zhang Zhao , Ma Nan , Gao Junping , Wu Hongzhi 
Published: December 2023

AbstractBotrytis cinerea is considered to be the second most destructive fungal pathogen worldwide, causing severe pre‐ and postharvest losses in cut roses. However, to date, no systematic research on its characteristics in cut roses has been reported. In our study, a total of 100 isolates from cut roses from Yunnan, China, were analysed. A combination of morphological characteristics and phylogenetic analysis of RPB2 revealed that 100 isolates were of the species B. cinerea. These isolates were pathogenic on unwounded detached rose petals. Pathogenicity was evaluated according to the size of petal spots and categorized into grades 0–5, from weak to strong. The inhibition rate varied greatly following the addition of procymidone (21.9%–100%) and cyprodinil (25.0%–92.3%). Analysis of Bc‐hch sequences revealed all isolates belong to phylogenetic Group II. According to the presence or absence of transposable elements, 87, 5, 4 and 4 isolates were identified as transposa, vacuma, Boty‐only and Flipper‐only types, respectively. Detection of mating type indicated that all isolates were heterothallic with 45% belonging to MAT1‐1 and 55% to MAT1‐2. Isolates were divided into four subpopulations when analysing single‐nucleotide polymorphisms at the genomic level, with a significant difference in pathogenicity between subpopulations 1 and 4. The evolutionary tree indicated that isolates AN‐02, AN‐22 and SM‐C18 were clustered in the root, suggesting an earlier evolutionary time than other isolates; moreover, they all exhibited low pathogenicity (grade 1). This systematic study of the characteristics of B. cinerea will provide significant support for grey mould control and further research.
Effect of experimental modulation of mood on exertional dyspnoea in chronic obstructive pulmonary disease
Authors: Sharma Pramod , Scheffer Karlijn , Louis Menaka , Aitken Craig R. , Adams Lewis , Morris Norman R. 
Published: December 2023

AbstractBackground and ObjectiveDyspnoea is a debilitating symptom in individuals with chronic obstructive pulmonary disease (COPD) and a range of other chronic cardiopulmonary diseases and is often associated with anxiety and depression. The present study examined the effect of visually‐induced mood shifts on exertional dyspnoea in individuals with COPD.MethodsFollowing familiarization, 20 participants with mild to severe COPD (age 57–79 years) attended three experimental sessions on separate days, performing two 5‐min treadmill exercise tests separated by a 30‐min interval on each day. During each exercise test, participants viewed either a positive, negative or neutral set of images sourced from the International Affective Picture System (IAPS) and rated dyspnoea or leg fatigue (0–10). Heart rate (HR) and peripheral oxygen saturation (SpO2) were measured at 1‐min intervals during each test. Mood valence ratings were obtained using Self‐Assessment Manikin (SAM) scale (1–9).ResultsMood valence ratings were significantly higher when viewing positive (end‐exercise mean ± SEM = 7.6 ± 0.3) compared to negative IAPS images (2.4 ± 0.3, p < 0.001). Dyspnoea intensity (mean ± SEM = 5.8 ± 0.4) and dyspnoea unpleasantness (5.6 ± 0.3) when viewing negative images were significantly higher compared to positive images (4.2 ± 0.4, p = 0.004 and 3.4 ± 0.5, p = 0.003). Eighty‐five percent of participants (n = 17) met the minimal clinically important difference (MCID) criteria for both dyspnoea intensity and unpleasantness. HR, SpO2 and leg fatigue did not differ significantly between conditions.ConclusionThese findings indicate that the negative affective state worsens dyspnoea in COPD, thereby suggesting strategies aimed at reducing the likelihood of negative mood or improving the mood may be effective in managing morbidity associated with dyspnoea in COPD.
Who Cashed the Rent Gap? An Alternative Narration of a Shantytown Renovation Project in Nanjing, China
Authors: Liu Chunhui , Yuan Yaqi , Qi Xiaoming 
Published: December 2023

AbstractDespite research on shantytown redevelopment highlighting socio‐spatial exclusion, the viewpoint frequently disseminated through social media depicts it as a massive wealth‐creation movement in China. This paper intends to provide evidence that evicted residents of a shantytown in Nanjing, China, share a common identity with the encroaching gentrifiers – the housing class. By shaping the housing class, an increasing number of urban residents become micro‐agents of housing financialization, allowing the rent gap to be frequently opened even without producing a ‘higher and better’ space. This enables capital to circulate and accumulate within a broader temporal and spatial scope. However, both the class imagination of the housing class and the financial speculation in the housing market are based on the expectation of continuous price growth. This is unsustainable and leaves the real housing needs out of the housing market, which is detrimental to housing equity.
Use of point‐of‐care haemoglobin tests to diagnose childhood anaemia in low‐ and middle‐income countries: A systematic review
Authors: Brehm Rebecca , South Annabelle , George Elizabeth C. 
Published: December 2023

AbstractObjectivesAnaemia is a major cause of mortality and transfusion in children in low‐ and middle‐income countries (LMICs); however, current diagnostics are slow, costly and frequently unavailable. Point‐of‐care haemoglobin tests (POC(Hb)Ts) could improve patient outcomes and use of resources by providing rapid and affordable results. We systematically reviewed the literature to investigate what, where and how POC(Hb)Ts are being used by health facilities in LMICs to diagnose childhood anaemia, and to explore challenges to their use.MethodsWe searched a total of nine databases and trial registries up to 10 June 2022 using the concepts: anaemia, POC(Hb)T, LMIC and clinical setting. Adults ≥21 years and literature published >15 years ago were excluded. A single reviewer conducted screening, data extraction and quality assessment (of diagnostic studies) using QUADAS‐2. Outcomes including POC(Hb)T used, location, setting, challenges and diagnostic accuracy were synthesised.ResultsOf 626 records screened, 41 studies were included. Evidence is available on the use of 15 POC(Hb)Ts in hospitals (n = 28, 68%), health centres (n = 9, 22%) and clinics/units (n = 10, 24%) across 16 LMICs. HemoCue (HemoCue AB, Ängelholm, Sweden) was the most used test (n = 31, 76%). Key challenges reported were overestimation of haemoglobin concentration, clinically unacceptable limits of agreement, errors/difficulty in sampling, environmental factors, cost, inter‐observer variability and supply of consumables. Five POC(Hb)Ts (33%) could not detect haemoglobin levels below 4.5 g/dL. Diagnostic accuracy varied, with sensitivity and specificity to detect anaemia ranging from 24.2% to 92.2% and 70% to 96.7%, respectively.ConclusionsPOC(Hb)Ts have been successfully utilised in health facilities in LMICs to diagnose childhood anaemia. However, limited evidence is available, and challenges exist that must be addressed before wider implementation. Further research is required to confirm accuracy, clinical benefits and cost‐effectiveness.
Plant nucleoside N‐ribohydrolases: riboside binding and role in nitrogen storage mobilization
Authors: Ľuptáková Eva , Vigouroux Armelle , Končitíková Radka , Kopečná Martina , Zalabák David , Novák Ondřej , Salcedo Sarmiento Sara , Ćavar Zeljković Sanja , Kopečný David Jaroslav , von Schwartzenberg Klaus , Strnad Miroslav , Spíchal Lukáš , De Diego Nuria , Kopečný David , Moréra Solange 
Published: December 2023

SUMMARYCells save their energy during nitrogen starvation by selective autophagy of ribosomes and degradation of RNA to ribonucleotides and nucleosides. Nucleosides are hydrolyzed by nucleoside N‐ribohydrolases (nucleosidases, NRHs). Subclass I of NRHs preferentially hydrolyzes the purine ribosides while subclass II is more active towards uridine and xanthosine. Here, we performed a crystallographic and kinetic study to shed light on nucleoside preferences among plant NRHs followed by in vivo metabolomic and phenotyping analyses to reveal the consequences of enhanced nucleoside breakdown. We report the crystal structure of Zea mays NRH2b (subclass II) and NRH3 (subclass I) in complexes with the substrate analog forodesine. Purine and pyrimidine catabolism are inseparable because nucleobase binding in the active site of ZmNRH is mediated via a water network and is thus unspecific. Dexamethasone‐inducible ZmNRH overexpressor lines of Arabidopsis thaliana, as well as double nrh knockout lines of moss Physcomitrium patents, reveal a fine control of adenosine in contrast to other ribosides. ZmNRH overexpressor lines display an accelerated early vegetative phase including faster root and rosette growth upon nitrogen starvation or osmotic stress. Moreover, the lines enter the bolting and flowering phase much earlier. We observe changes in the pathways related to nitrogen‐containing compounds such as β‐alanine and several polyamines, which allow plants to reprogram their metabolism to escape stress. Taken together, crop plant breeding targeting enhanced NRH‐mediated nitrogen recycling could therefore be a strategy to enhance plant growth tolerance and productivity under adverse growth conditions.
GARCH Models and Distributions Comparison for Nonlinear Time Series with Volatilities
Authors: Abdul Rahman Nur Haizum , Jia Goh Hui , Zulkafli Hani Syahida 
Published: December 2023

The Generalized Autoregressive Conditional Heteroskedasticity (GARCH) model is extensively used for handling volatilities. However, with numerous extensions to the standard GARCH model, selecting the most suitable model for forecasting price volatilities becomes challenging. This study aims to examine the performance of different GARCH models in forecasting crude oil price volatilities using West Texas Intermediate (WTI) data. The models considered are the standard GARCH, Integrated GARCH (IGARCH), Exponential GARCH (EGARCH), and Golsten, Jagannathan, and Runkle GARCH (GJR-GARCH), each with normal distribution, Student’s t-distribution, and Generalized Error Distribution (GED). To evaluate the performance of each model, the Akaike Information Criteria (AIC) and Bayesian Information Criteria (BIC) are used as the model selection criteria, along with forecast accuracy measures such as absolute error, root mean squared error (RMSE), and mean absolute error (MAE). Post-estimation tests, including the Autoregressive Conditional Heteroskedasticity Lagrange Multiplier (ARCH-LM) test and the Ljung-Box test, are conducted to ensure the adequacy of all models. The results reveal that all GARCH models are suitable for modeling the data, as indicated by statistically significant estimated parameters and satisfactory post-estimation outcomes. However, the EGARCH (1, 1) model, particularly with Student’s t-distribution, outperforms other models in both data fitting and accurate forecasting of nonlinear time series.
Molnupiravir maintains antiviral activity against SARS-CoV-2 variants and exhibits a high barrier to the development of resistance
Authors: Strizki Julie M. , Gaspar John M. , Howe John A. , Hutchins Beth , Mohri Hiroshi , Nair Manoj S. , Kinek Keith C. , McKenna Philip , Goh Shih Lin , Murgolo Nicholas 
Published: December 2023

ABSTRACT

Molnupiravir, an oral prodrug of N-hydroxycytidine (NHC), previously demonstrated broad
in vitro
antiviral activity against multiple RNA viruses and has shown a high barrier to the development of resistance. Here, we present the antiviral activity of NHC against recent SARS-CoV-2 variants and the results of resistance selection studies to better understand the potential for viral resistance to NHC. NHC activity against SARS-CoV-2 variants omicron (BA.1, BA.1.1, BA.2, BA.4, BA.4.6, BA.5, BQ.1.1, XBB.1, and XBB.1.5), alpha (B.1.1.7), beta (B.1.351), gamma (P.1), delta (B.1.617.2), lambda (C.37), and mu (B.1.621) was evaluated in Vero E6 cells using cytopathic effect assays. Resistance selection studies were performed by passaging SARS-CoV-2 (WA1) in the presence of NHC or a 3C-like protease inhibitor (MRK-A) in Vero E6 cells. Supernatants from cultures exhibiting a cytopathic effect score of ≥2 were re-passaged, and IC
50
values were estimated. Whole-genome deep sequencing was performed on viral RNA isolated at each passage. NHC demonstrated similar potency against all SARS-CoV-2 variants evaluated. No evidence of SARS-CoV-2 phenotypic or genotypic resistance to NHC was observed following 30 passages. A random pattern of nucleotide changes was observed in NHC cultures, consistent with the drug’s mechanism of action. In contrast, resistance was readily selected in all three MRK-A control cultures with the selection of a T21I substitution in the 3C-like protease. In conclusion, molnupiravir maintains antiviral activity across all major SARS-CoV-2 variants. Furthermore, no evidence of viral resistance to NHC was observed, supporting previous reports that NHC has a high barrier to developing resistance.
Relative inhibitory activities of the broad-spectrum β-lactamase inhibitor taniborbactam against metallo-β-lactamases
Authors: Le Terrier Christophe , Viguier Clément , Nordmann Patrice , Vila Alejandro J. , Poirel Laurent 
Published: December 2023

ABSTRACT
Taniborbactam (TAN) is a novel broad-spectrum β-lactamase inhibitor with significant activity against subclass B1 metallo-β-lactamases (MBLs). Here, we showed that TAN exhibited an overall excellent activity against B1 MBLs including most NDM- and VIM-like as well as SPM-1, GIM-1, and DIM-1 enzymes, but not against SIM-1. Noteworthy, VIM-1-like enzymes (particularly VIM-83) were less inhibited by TAN than VIM-2-like. Like NDM-9, NDM-30 (also differing from NDM-1 by a single amino acid substitution) was resistant to TAN.