bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relaxin Modifies Systemic Arterial Resistance and Compliance in Conscious, Nonpregnant Rats
Authors: Conrad Kirk P. , Debrah Dan O. , Novak Jackie , Danielson Lee A. , Shroff Sanjeev G. 
Published: June 2004

Abstract
Relaxin emanates from the corpus luteum of the ovary and circulates during pregnancy. Because the hormone is a potent renal vasodilator and mediates the renal vasodilation and hyperfiltration of pregnancy in conscious rats, we reasoned that it might also contribute to the broader cardiovascular changes of pregnancy. We began investigating this concept by testing whether relaxin can modify systemic arterial hemodynamics and load when chronically administered to nonpregnant rats. The major objectives of the present work were to determine whether relaxin administration to nonpregnant rats 1) modifies cardiac output (CO), systemic vascular resistance, and global arterial compliance (AC), and 2) regulates the passive mechanics of isolated arteries. To accomplish the first objective, we developed a conscious rat model for assessment of global AC. Passive mechanics of small renal arteries were assessed using a pressure arteriograph. Chronic administration of recombinant human relaxin by sc osmotic minipump to conscious, female, nonpregnant rats reduced the steady arterial load by decreasing systemic vascular resistance, increased CO, and reduced the pulsatile arterial load by increasing global AC as quantified by two indices—AC estimated from the diastolic decay of aortic pressure and CO and AC estimated by the ratio of stroke volume-to-pulse pressure. In another group of rats, relaxin administration also regulated the passive mechanics of small renal arteries, indicating that, in addition to reduction in vascular smooth muscle tone, modification of the vascular structure (e.g. extracellular matrix) contributes to the increase in global AC. These findings suggest a role for relaxin in the systemic hemodynamic changes of pregnancy, as well as novel therapeutic potential for relaxin in modifying arterial stiffness and cardiac afterload.
Local action of exogenous nitric oxide (NO) on the skin blood flow of rock pigeons (Columba livia) is affected by acclimation and skin site
Authors: Peltonen Liisa M. , Pyörnilä Ahti 
Published: June 2004

SUMMARY
We studied the blood flow over dorsal and abdominal, non-brooding patch skin of two groups of pigeons: one group was thermally acclimated to cold(winter-acclimatized, WAC) while the other group was acclimated to a mesic environment (thermally non-challenged, NOC). Two bilateral patches at the measurement sites were treated simultaneously with a gel containing sodium nitrate and ascorbic acid, to generate nitric oxide (NO), and a vehicle gel. Blood flow was measured by laser Doppler velocimetry. Changes induced by these treatments were calculated against basic blood flow values for the corresponding patch. The results showed that the basic blood flow over the abdominal skin patches at room temperature was higher than over the dorsal skin in both acclimation states, but comparison revealed a sustainably higher level of basic skin blood flow in the WAC pigeons. The local response to exogenous NO was non-uniform over the two skin areas measured, and a specific vasodilatory action on the abdominal microvessels was recorded in the NOC pigeons. Abdominal vasodilatation in the WAC pigeons seemed to involve other mechanisms as well as local NO-dependent ones, among which the role of cold-induced vasodilatation (CIVD) is discussed here. Interestingly, the dorsal skin seemed to be less responsive to the action of NO, irrespective of the acclimation state.
Our results show that acclimation state and skin site affect the action of exogenous NO on local skin blood flow, and we suggest that the differences reflect acclimation-induced changes in the vascularity of the skin and in its sensitivity to thermal stimuli and that the roles of the abdominal and dorsal skin are different with respect to environmental changes.
Cardiac plasticity in fishes: environmental influences and intraspecific differences
Authors: Gamperl A. Kurt , Farrell A. P. 
Published: June 2004

SUMMARYFish cardiac physiology and anatomy show a multiplicity of intraspecific modifications when exposed to prolonged changes in environmentally relevant parameters such as temperature, hypoxia and food availability, and when meeting the increased demands associated with training/increased activity and sexual maturation. Further, there is evidence that rearing fish under intensive aquaculture conditions significantly alters some, but not all,aspects of cardiac anatomy and physiology. This review focuses on the responses of cardiac physiology and anatomy to these challenges, highlighting where applicable, the importance of hyperplastic (i.e. the production of new cells) vs hypertrophic (the enlargement of existing cells) growth to the adaptive response of the heart. In addition, we summarize recent studies that have explored the relationship between the myocardial protection afforded by preconditioning and myocardial hypoxia tolerance. This latter research clearly demonstrates the capacity of the fish heart to adjust to short-term perturbations, and shows that it can be difficult to predict how short-term and long-term alterations in cardiac physiology will interact.
Basolateral ion transport mechanisms during fluid secretion byDrosophilaMalpighian tubules: Na+ recycling,Na+:K+:2Cl– cotransport and Cl– conductance
Authors: Ianowski Juan P. , O'Donnell Michael J. 
Published: June 2004

SUMMARYMechanisms of ion transport during primary urine formation by the Malpighian tubule of Drosophila melanogaster were analyzed through measurements of fluid secretion rate, transepithelial ion flux, basolateral membrane potential (Vbl) and intracellular activities of K+ (aKi) and Cl–(aCli). Calculation of the electrochemical potentials for both ions permitted assessment of the possible contributions of K+ channels, Na+:K+:2Cl–cotransport, and K+:Cl– cotransport, to net transepithelial ion secretion across the basolateral membrane. The data show that passive movement of both K+ and Cl– from cell to bath is favoured across the basolateral membrane, indicating that both ions are actively transported into the cell. Contributions of basolateral K+ channels or K+:Cl– cotransporters to net transepithelial ion secretion can be ruled out. After prior exposure of tubules to ouabain, subsequent addition of bumetanide reduced fluid secretion rate, K+ flux and Na+ flux, indicating a role for a Na+:K+:2Cl– cotransporter in fluid secretion. Addition of the K+ channel blocker Ba2+ had no effect on aKi or aCli. Addition of Ba2+ unmasked a basolateral Cl– conductance and the hyperpolarization of Vbl in response to Ba2+ was Cl–-dependent. A new model for fluid secretion proposes that K+ and Cl– cross the basolateral membrane through a Na+-driven Na+:K+:2Cl–cotransporter and that most of the Na+ that enters the cells is returned to the bath through the Na+/K+-ATPase.
Antennae on transmitters on penguins: balancing energy budgets on the high wire
Authors: Wilson Rory P. , Kreye Jan M. , Lucke Klaus , Urquhart Heather 
Published: June 2004

SUMMARYThe effect of externally mounted antennae on the energetics of penguins was studied by mounting various antennae on a transducer fixed to a model Magellanic penguin Spheniscus magellanicus to determine drag, run at speeds of up to 2 m s–1 in a swim canal. For rigid antennae set perpendicular to the water flow, measured drag increased with increasing swim speed. Increasing antenna length (for lengths between 100 and 200 mm) or diameter (for diameters between 1 and 4 mm) resulted in accelerating increased drag as a function of both antenna length and diameter. Where antennae were positioned at acute angles to the water flow, drag was markedly reduced, as was drag at higher speeds in flexible antennae. These results were incorporated in a model on the foraging energetics of free-living Magellanic penguins using data (on swim speeds, intervals between prey encounters, amount ingested per patch and dive durations) derived from previously published work and from a field study conducted on birds from a colony at Punta Norte,Argentina, using data loggers. The field work indicated that free-living birds have a foraging efficiency (net energy gain/net energy loss) of about 2.5. The model predicted that birds equipped with the largest rigid external antennae tested (200 mm × 3 mm diameter), set perpendicular to water flow,increased energy expenditure at normal swim speeds of 1.77 m s–1 by 79% and at prey capture speeds of 2.25 m s–1 by 147%, and ultimately led to a foraging efficiency that was about 5 times less than that of unequipped birds. Highly flexible antennae were shown to reduce this effect considerably. Deleterious antenna-induced effects are predicted to be particularly critical in penguins that have to travel fast to capture prey. Possible measures taken by the birds to increase foraging efficiency could include reduced travelling speed and selection of smaller prey types. Suggestions are made as to how antenna-induced drag might be minimized for future studies on marine diving animals.
Colourful orb-weaving spiders, Nephila pilipes, through a bee's eyes
Authors: Tso I-Min , Lin Chih-Wei , Yang En-Cheng 
Published: June 2004

SUMMARY
Many orb-weaving spiders in the tropics forage in open sites during the day and some of them have both bright and dark colourations. The conspicuous UV-reflective colour markings of these spiders have been reported to be attractive to visually oriented prey and thus could increase the spiders'foraging success. Using a combination of field and laboratory studies, we examine whether or not the body colouration of orb-weaving spiders exhibits optical properties that are attractive to insect prey from the viewpoint of insect visual physiology. We compared the prey interception rates and colour contrasts of the typical and melanic morphs of the giant wood spider, Nephila pilipes. Results of the field study showed that the typical morph caught significantly more insects than the melanic morph. Colour contrasts calculated from spectral reflectances of the background and body surface of spiders showed that the brightly coloured body parts of the typical morph exhibited rather high values, but those of the dark body parts were below the discrimination threshold. The differential colour contrasts of body parts generated a visual signal unlike that of a spider but rather like certain forms of food resources. On the other hand, the melanic morphs did not have bright colouration and the colour contrasts of every part of the body were significantly higher than the threshold, making the contour of spiders quite clear to bees.
Dopaminergic and serotonergic innervation of cockroach salivary glands:distribution and morphology of synapses and release sites
Authors: Baumann Otto , Kühnel Dana , Dames Petra , Walz Bernd 
Published: June 2004

SUMMARYThe paired salivary glands in the cockroach are composed of acini with ion-transporting peripheral P-cells and protein-secreting central C-cells, and a duct system for the modification of the primary saliva. Secretory activity is controlled by serotonergic and dopaminergic neurons, whose axons form a dense plexus on the glands. The spatial relationship of release sites for serotonin and dopamine to the various cell types was determined by anti-synapsin immunofluorescence confocal microscopy and electron microscopy. Every C-cell apparently has only serotonergic synapses on its surface. Serotonergic and dopaminergic fibres on the acini have their release zones at a distance of ∼0.5 μm from the P-cells. Nerves between acinar lobules may serve as neurohaemal organs and contain abundant dopaminergic and few serotonergic release sites. Some dopaminergic and serotonergic release sites reside in the duct epithelium, the former throughout the duct system, the latter only in segments next to acini. These findings are consistent with the view that C-cells respond exclusively to serotonin, P-cells to serotonin and dopamine, and most duct cells only to dopamine. Moreover, the data suggest that C-cells are stimulated by serotonin released close to their surface,whereas P-cells and most duct cells are exposed to serotonin/dopamine liberated at some distance.
Purification of a soluble glycoprotein from the uncalcified ecdysial cuticle of the blue crabCallinectes sapidusand its possible role in initial mineralization
Authors: Tweedie Elizabeth P. , Coblentz Francie E. , Shafer Thomas H. 
Published: June 2004

SUMMARYA heavily glycosylated soluble protein was purified using a combination of lectin affinity and size exclusion chromatography from a soluble extract of uncalcified dorsal cuticle of blue crab Callinectes sapidus removed at ecdysis. Similarities in apparent molecular mass and carbohydrate composition suggest that this protein is the same species previously shown to disappear from soluble extracts coincidentally with the onset of mineral deposition in the newly exposed post-molt cuticle. The amino acid sequence of the N-terminal portion of the core polypeptide was determined and polyclonal antibodies were raised against both the purified glycoprotein and the peptide. Immunoblots of unfractionated soluble extracts taken at various times post-molt illustrated that the anti-peptide antibody recognized several polypeptides with electrophoretic mobilities that differ from the purified glycoprotein. These bands may be deglycosylation products which would not have been purified due to different lectin affinity or size. Immunohistochemical analysis indicated uniform protein distribution in the exocuticle at ecdysis,but decreased antibody binding at the interprismatic septa by 2 h post-molt. The location of the protein is therefore the negative image of the calcification pattern in the exocuticle and provides a spatial pattern to correlate with the previously reported temporal events. This strengthens the hypothesis that the glycoprotein under investigation is an inhibitor of calcite nucleation or of initial amorphous calcium carbonate accumulation.
Developmental allometry of pulmonary structure and function in the altricial Australian pelican Pelecanus conspicillatus
Authors: Seymour Roger S. , Runciman Sue , Baudinette Russell V. , Pearson James T. 
Published: June 2004

SUMMARY
Quantitative methods have been used to correlate maximal oxygen uptake with lung development in Australian pelicans. These birds produce the largest altricial neonates and become some of the largest birds capable of flight. During post-hatching growth to adults, body mass increases by two orders of magnitude (from 88 g to 8.8 kg). Oxygen consumption rates were measured at rest and during exposure to cold and during exercise. Then the lungs were quantitatively assessed using morphometric techniques. Allometric relationships between body mass (M) and gas exchange parameters(Y) were determined and evaluated by examining the exponents of the equation Y=aMb. This intraspecific study was compared to interspecific studies of adult birds reported in the literature. Total lung volume scales similarly in juvenile pelicans (b=1.05) as in adult birds (b=1.02). However, surface area of the blood–gas barrier greatly increases (b=1.25), and its harmonic mean thickness does not significantly change (b=0.02), in comparison to exponents from adult birds (b=0.86 and 0.07, respectively). As a result, the diffusing capacity of the blood–gas tissue barrier increases much more during development (b=1.23) than it does in adult birds of different sizes (b=0.79). It increases in parallel to maximal oxygen consumption rate (b=1.28), suggesting that the gas exchange system is either limited by lung development or possibly symmorphic. The capacity of the oxygen delivery system is theoretically sufficient for powered flight well in advance of the bird's need to use it.
Learning and memory inLymnaeaare negatively altered by acute low-level concentrations of hydrogen sulphide
Authors: Rosenegger David , Roth Sheldon , Lukowiak Ken 
Published: June 2004

SUMMARYHydrogen sulphide (H2S) is a common industrial pollutant as well as an endogenous neural transmitter/neural modulator. Experiments were performed on the pond snail Lymnaea stagnalis to determine the acute effects of low-level exposure to H2S (50–100 μmol l–1) on aerial respiratory behaviour, associative learning,and its subsequent consolidation into long-term memory (LTM). A 3-neuron network whose sufficiency and necessity have been demonstrated drives aerial respiratory behaviour in Lymnaea. In the presence of 100 μmol l–1 H2S the number of bouts of aerial respiration and the total breathing time were significantly increased compared to the control hypoxic situation, but were equivalent to those observed in snails that had been subjected to a `more intense hypoxic challenge'. In addition, at a concentration of 100 μmol l–1 H2S neither associative learning nor long-term memory (LTM) were observed. However, snails subjected to a `more intense hypoxic challenge' still had the capacity to learn and form LTM. These snails, in fact, showed statistically the best learning and memory performance of any group. While learning and memory were observed at 50 and 75 μmol l–1 H2S,respectively, they were statistically poorer than the learning and memory exhibited by snails in the standard hypoxia condition. Hence the ability to learn and form memory was compromised by H2S. Thus an invertebrate model system with a well-defined neural network can be used to study of the effects of H2S on the processes of learning and memory.
Adjustments of gastric pH, motility and temperature during long-term preservation of stomach contents in free-ranging incubating king penguins
Authors: Thouzeau C. , Peters G. , Le Bohec C. , Le Maho Y. 
Published: June 2004

SUMMARY
Male king penguins are able to store undigested food in their stomach for up to 3 weeks during their incubation fast, which evidently implies some modification of their digestive process. Using small electronic recorders, we studied the change in gastric pH, motility and temperature during the first week of food storage. The pH could be maintained at values as high as 6 throughout the incubation fast, a pH that is unfavourable for avian gastric proteinase activity. Gastric motility was never completely inhibited but could be markedly reduced. Stomach temperature was maintained at around 38°C. The fact that stomach temperature of incubating birds did not show a daily rhythmic fluctuation as seen in non-breeding birds could be due to temperature constraints on embryo development. Thus the present study demonstrates substantial adjustments of pH and gastric motility in incubating king penguins, which may contribute to the inhibition of digestive gastric processes.
The genes for the helix-loop-helix proteins Id6a, Id6b, Id1 and Id2 are specifically expressed in the ventral and dorsal domains of the fish developing somites
Authors: Rallière Cécile , Chauvigné François , Rescan Pierre-Yves 
Published: June 2004

SUMMARYMuscle differentiation is inhibited by members of the Id family that block the transcriptional effect of myogenic bHLH regulators by forming inactive heterodimers with them. Also, Id proteins promote cell proliferation by interacting with key regulators of the cell cycle. In order to determine the role of Id-encoding genes during fish development and especially in early myogenesis, we examined the expression patterns of Id1, Id2 and two nonallelic Id6 (Id6a and Id6b)-encoding genes in developing trout embryos. These four Id paralogs were found to exhibit discrete expression in the developing nervous system and in the eye rudiment. During the segmentation process, Id6a, Id6b and Id1 were expressed in the tail bud, the paraxial mesoderm and the ventral and dorsal domains of neoformed somites. As the somite matured in a rostrocaudal progression, the labelling for Id1 transcripts rapidly faded whereas labelling for Id6 transcripts was found to persist until at least the completion of segmentation. By contrast, Id2 transcripts were visualised transiently only in dorsal domains of neoformed somites and strongly accumulated in the pronephros. The preferential localisation of Id6a, Id6b, Id1 and Id2 transcripts within ventral and/or dorsal extremes of the developing somites, suggests that these areas, which were the last ones to express muscle-specific genes, contain dividing cells involved in somite expansion.
Reduced juvenile hormone synthesis in mosquitoes with low teneral reserves reduces ovarian previtellogenic development in Aedes aegypti
Authors: Caroci Abrahim S. , Li Yiping , Noriega Fernando G. 
Published: June 2004

SUMMARY
We investigated the relationship among nutritional reserves,previtellogenic ovary development and juvenile hormone (JH) synthesis in Aedes aegypti female mosquitoes. By raising larvae under different nutritional regimes, two adult phenotypes (large and small females) were generated, which differed significantly in size at eclosion (measured by wing length). We measured the total amount of protein, lipids and glycogen in newly emerged (teneral) large and small females. Teneral reserves were significantly lower in small females. Maximum previtellogenic ovary development occurred only if enough teneral nutrients were present. Maximum previtellogenic ovary development was stimulated in small females with low teneral nutrients by topically applying a JH analog. The biosynthetic activity of Ae. aegypti corpora allata (CA) was studied in vitro using a radiochemical method. JH synthesis was significantly reduced in females emerged with low teneral reserves and stimulated by sugar feeding. These results establish that the CA synthesizes enough JH to activate ovary maturation only in the presence of large nutrient reserves.
Wing hair sensilla underlying aimed hindleg scratching of the locust
Authors: Page Keri L. , Matheson Thomas 
Published: June 2004

SUMMARYThe anatomy and physiology of exteroceptors on the surfaces of the wings have been described in many insects, but their roles in behaviour have been less well studied. They have often been assumed to have a role primarily in flight. We show that the wings of the locust Schistocerca gregariapossess at least three different hair types with characteristic patterns of distribution that determine the probability of eliciting targeted hindleg scratching behaviour.The different hair types are defined by their morphology and innervation. The shortest hairs (14–46 μm) are basiconic receptors containing both chemosensory and mechanosensory afferents. They are distributed widely across the dorsal surfaces of the forewings; some are located on the ventral surfaces of the hindwings, but none are found on the ventral surfaces of the forewings or the dorsal surfaces of the hindwings. Medium length hairs (73–159μm) are found on all wing surfaces, but are restricted to the veins,principally the subcosta on the dorsal surface of the forewings. The longest hairs (316–511 μm) are found only on the postcubitus vein on the dorsal surfaces of the forewings, so that they form a pair of dorsal rows when the wings are folded at rest.Touching the dorsal surface of a forewing can elicit aimed scratching movements of a hindleg, and we show that the probability of eliciting a scratch differs for different stimulus sites and for different start positions of the hind leg. The effectiveness of different stimulus sites can be correlated with the distribution of tactile hairs on the dorsal forewing surface. Touching the long hairs provides the strongest drive to elicit a scratch, and ablating them reduces the probability to almost zero. We conclude that input from forewing tactile hairs plays an important role in eliciting hindleg scratching and encodes the spatial location required for targeting.