bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Omics Analysis Reveals Cold-Induced Metabolic Reprogramming and Alternative Splicing in Dendrobium officinale
Authors: Zhan Xinqiao , Li Zhangqun , Pang Minxia , Yao Guoxiang , Mao Bizeng 
Published: January 2025

Dendrobium officinale is an economically important orchid species that is sensitive to cold stress. Understanding the molecular and metabolic mechanisms underlying its response to cold is crucial for developing strategies to improve its cold tolerance. In this study, we constructed a comprehensive cold stress response dataset for D. officinale and characterized its regulatory landscape in response to varying cold stress conditions. The glycine metabolism-related genes Dca003913 and Dca022726 play pivotal roles in both cold and drought stress adaptation, and their expression is not upregulated by hormones or fungi infection. Carbohydrate metabolism showed specific dynamic changes in freezing injury cells, which involved a variety of hormonal responses. The abundance of sphingolipids was notably higher in the freezing treatment (FT) compared to the freezing recovery (FR) plants, indicating specialized metabolic adaptations at different cold intensities. An alternative splicing (AS) analysis identified 368 DAS genes, with spliceosome pathways significantly enriched. Three key ubiquitination proteins (PKU64802, XP_020672210, and PKU75555) were found to regulate splicing factors, which showed increased abundance in cold stress. This study highlights the roles of metabolic reprogramming and RNA splicing in cold adaptation, revealing a complex molecular network activated in response to cold stress.
OPTIMAL CONDITIONS FOR EXTRACTION PHENOLIC COMPOUNDS AND FLAVONOIDS FROM MILLET BRAN AND STUDYING SOME OF THEIR BIOLOGICAL ACTIVITY
Authors: Mariem M. A.AL-Abas  , Jassem. M. Nasser  
Published: January 2025

The aim of this research is to determine the optimal conditions for extracting phenols and flavonoids from the bran of Iraqi proso millet and Ukrainian foxtail millet, whole and defatted, and studying some of their biological characteristics. Petroleum ether, methyl alcohol (60% and 80%), distilled water, and ethyl alcohol at concentrations of 60, 80, 90, and 99% were used as extraction solutions. The flavonoid content was estimated using the Rutin standard curve, and the phenolic content was determined using the Folin-Ciocateau method based on the gallic acid standard curve. The results showed that the concentration of phenolic compounds and flavonoids in proso millet bran extracts were (770.56, 865.38, 1059.94, 883.16, 722.17, 726.12, 587.79, 400.69) µg/g and (188.26, 196.26, 353.60, 671.38, 96.94, 93.60, 117.60, 300.60) µg /g, respectively, and in Ukrainian millet bran extracts (889.08, 1034.80, 1182.00, 1331.17, 1008.19, 990.42, 592.85, 581.43) µg /g and (302.94, 484.26, 758.94, 940.32, 188.26, 97.60, 217.60, 554.94) µg/g respectively. The highest phenolic and flavonoids compounds concentrations were achieved when ethyl alcohol (99%) as extraction solution was used for Ukrainian millet. Therefore, this treatment was adopted in conducting the free radical scavenging test, at different concentrations (0.125, 0.250, 0.500, 0.750, 0.800) mg/ml, as RSA were (24.68, 40.18, 67.56, 81.98, 89.55%), respectively, compared to ascorbic acid at the same concentrations, which were (40.14, 57.34, 73.46, 89.93, 99.55%).
Wściekłość i wrzask. Studium recepcji „Ładunku 200” Aleksieja Bałabanowa
Authors: Gorlewska Paulina 
Published: January 2025

Przedmiotem artykułu jest analiza rosyjskiej recepcji Ładunku 200 (Gruz 200, reż. Aleksiej Bałabanow, 2007), która miała miejsce tuż po premierze utworu. Ocena filmu jako utworu artystycznego oraz prawdziwości zawartego w nim obrazu Rosji podzieliła zarówno krytyków, jak i zwykłych odbiorców, stając się tematem debaty społecznej. Analityczna część tekstu, dotycząca interpretacji krytycznych dzieła, jest oparta na historyczno-materialistycznej metodzie badania odbioru utworów filmowych opracowanej przez Janet Staiger. Punktem odniesienia jest w niej ideologia dominująca w polityce historycznej ówczesnej Rosji oraz w klasycznych regułach narracji. Do analizy afektywnych doświadczeń odbiorczych została użyta teoria psychoanalityczna.
Bird assemblage structure in the Desert Puna in areas with different disturbance activity of a subterranean herbivorous rodent
Authors: Andino Natalia , Fernández Viviana , Giannoni Stella M. , Borghi Carlos E. 
Published: January 2025

Herbivorous subterranean rodents, such as Ctenomys mendocinus (tuco-tuco), have a significant impact on vegetation cover and composition. This study aimed to examine the indirect influence of tuco-tucos on bird populations and assemblages in the southernmost region of the desert Puna. In a shrub-steppe community, at an altitude of between 3,000 and 3,100 m.a.s.l., bird surveys were conducted from February 2001 to October 2004. Transects of 50 m in length and 40 m in width were established: 56 located in highly disturbed patches and 53 in patches with low disturbance by tuco-tucos. Our results show that areas with higher disturbance by tuco-tucos are associated with a decrease in bird abundance (93 individuals in disturbed areas vs. 226 in undisturbed areas) and lower species richness (6 species in disturbed areas vs. 11 in undisturbed areas).
Análisis de las relaciones eco-geomorfológicas y edáficas a lo largo de un transecto en un sector de alta montaña del Parque Nacional Sierra de las Nieves
Authors: Martínez-Murillo Juan F. , Menjíbar-Romero Mario , Carruana-Herrera David , Hueso-González Paloma 
Published: January 2025

El Parque Nacional Sierra de las Nieves se sitúa en un espacio geográfico hotspot del cambio global. Este espacio presenta una gran variedad de paisajes eco-geomorfológicos, siendo el menos estudiado el situado por encima de 1.600 m.s.n.m., con rasgos bioclimáticos de alta montaña y uso humano prolongado. Este trabajo estudia las relaciones eco-geomorfológicas y edáficas en este sector, mediante la realización de un inventario eco-geomorfológico y muestreo y análisis de propiedades edáficas a lo largo de un transecto longitudinal, en una depresión de laderas calcáreas y presencia de cárcavas de fondo. Los resultados evidencian la falta de relación estadística entre las variables consideradas, lo que manifiesta la existencia de un patrón eco-geomorfológico que podría ser similar al de las islas de fertilidad, característica de ambientes semiáridos, a consecuencia de las condiciones bioclimáticas y el impacto del uso humano heredado. Este hallazgo deberá ser investigado con mayor detenimiento en futuros trabajos.
Femmes de sciences
Authors: Cabanel Anna 
Published: January 2025

Femme et science : les deux termes ont longtemps été perçus comme antinomiques, si bien que dans l’imaginaire des sociétés occidentales la figure du scientifique demeure encore largement masculine. Transformer cette image afin que les femmes puissent être pleinement acceptées et reconnues dans les mondes scientifique et universitaire est le défi qu’a voulu relever l’International Federation of University Women (Fédération internationale des femmes diplômées des universités ou FIFDU). Fondée en 1919 par des femmes scientifiques de renom international, la FIFDU est la première organisation scientifique exclusivement féminine ; elle parvient à s’imposer, au cours de l’entre-deux-guerres, sur la scène internationale.À la croisée des champs de l’histoire des sciences et de l’histoire du genre, l’ouvrage s’intéresse aux processus d’élaboration et de promotion d’une figure scientifique déclinée au féminin : celle des university women. Il analyse les efforts menés par la FIFDU, spécialement la mise en place d’un programme international de bourses de recherche dédié aux chercheuses, pour déconstruire les conventions sociales et les représentations culturelles qui ralentissent la participation et la reconnaissance des femmes en science.
Comparing the Effectiveness of ChatGPT3.5 and Bing GPT4 as Supplementary Resources to Enhance the Teaching, Learning, and Assessment of Human Anatomy
Authors: Wickramarathna A. M. , Kumara S. S. , Buddhasinghe R. U. , Wijayasekara A. E. 
Published: January 2025

Introduction: Artificial intelligence (AI) has shown promise in revolutionizing healthcare education by providing efficient and beneficial learning opportunities for students and reducing the workload for educators. Materials and methods: This study was conducted from September 2023 to October 2023 assessing the accuracy, relevance, comprehensiveness, and user-friendliness of responses given by ChatGPT3.5 and Bing GPT4 on human anatomy. Results: Both AI tools were able to provide detailed descriptions of gross anatomy, human embryology, and histology. While both showed a need for improvement in generating sample questions, Traditional methods still offer superior accuracy and relevance compared to AI Chatbots. But Chatbots provide personalized and convenient responses. Conclusion: As AI technology continues to evolve, caution should be exercised in using AI tools for anatomy education, as their capabilities and performance may change over time.
Can a Projected Image Test Replace the Traditional Gross Anatomy Spot Test?
Authors: Amaratunga H. A. , Kosgallana W. E. 
Published: January 2025

Introduction: Conducting traditional gross anatomy spot (spotter/tag) (3D) in the laboratory is becoming increasingly difficult due to the large number of students and shortage of staff. This study aimed to assess the practicality of conducting projected gross anatomy image test (2D) conducted in a lecture hall for first year medical undergraduates and assessing their performance and attitudes towards this form of testing. Material and Methods: First-year medical undergraduates were subject to two forms of gross anatomy testing in the form of traditional spot test (3D) and projected gross anatomy image test (2D). An online questionnaire was administered to the students to gain information regarding their attitudes regarding the two types of examinations. Descriptive analysis and one-sample T-test was used to compare the results . Results: Sample consisted of 248 students. There were 149 females (60%). Mean age in years was 21.34 (SD=2.3). The mean projected image test scores out of hundred was 70% (SD=3.14). The mean score for the OSPE component of the Y1S1 examination was 63.3% (SD=4.11). The observe difference in the means was statistically significant (p<0.001). Conclusion: While the projected gross anatomy image test (2D) was less time consuming and easy to setup and the students performed better in it they prefer the gross anatomy spot (3D) type of test. Both types have their advantages and disadvantages and maybe used in combination to reduce examination time and invigilator requirements.
Undergraduate Medical Education in Bangladesh: Current Scenario and Future Challenges
Authors: Khan K. M. , Ferdous J. , Islam M. 
Published: January 2025

Introduction: Undergraduate medical education in Bangladesh plays a crucial role in developing competent physicians. The Bachelor of Medicine and Bachelor of Surgery (MBBS) is the primary undergraduate medical degree. The medical education system in Bangladesh is structured around government and private medical colleges, with the former being more accessible due to lower tuition fees. Objective: This country paper highlighted the current situation, future challenges and recommendations to ensure quality of undergraduate medical education in Bangladesh. Current scenario and challenges: Though the landscape of undergraduate medical education in Bangladesh has evolved significantly over the past few decades, it still faces various challenges in terms of curriculum, infrastructure, quality control and disparities between government and private institutions. One of the key issues is the mismatch between theoretical knowledge and practical skills, which has been a long-standing criticism in medical education globally. This gap is often attributed to a curriculum that has not fully embraced modern pedagogical approaches. Recommendations: To improve undergraduate medical education in Bangladesh, several key reforms are necessary. First, curriculum revision is crucial, focusing on competency-based education that emphasizes critical thinking, patient care and ethical practice. Second, faculty development programs should be expanded to include modern teaching strategies and continuous professional development. Third, both public and private medical colleges should invest in improving infrastructure and resources to support practical learning. Finally, stronger oversight by regulatory bodies is needed to ensure that medical colleges adhere to high educational standards.
Development and Validation of a Tool to assess the Retention of Clinical Anatomy Knowledge
Authors: Amaratunga H. A. , Dissabandara L. , Adikari S. B. , Marambe N. K. 
Published: January 2025

Introduction: A thorough knowledge in anatomy is essential for the medical student and thereafter for the practicing doctor. Therefore, retention of knowledge acquired during formative years becomes valuable. Knowledge attrition may occur as students move from preclinical years towards clinical training. A tool to measure the level of retention becomes important to assess the level of attrition and apply remedial measures. Methodology: AKRA tool, a MCQ based tool was developed aligned to the learning objectives assessed and edited by opinions of clinicians. The AKRA tool was validated using expert opinion and statistical methods. For statistical assessment, it was administered to 100, 3rd year medical students and 20 recently passed out doctors and the scores calculated. Reliability was assessed using Cronbach alpha, and item discrimination using Pearson correlation. MCQs were graded into poor, marginal, good and excellent based on item discrimination and difficulty indices. Criterion validity was assessed by comparing the AKRA scores with those of an institutional examination. AKRA score was administered to students of two other institutions and scores were compared. Results: AKRA tool consisted of 42 MCQs. The reliability was high (Cronbach Alph = 0.81). The average score for AKRA was 61.78 (SD=12.3) %. The AKRA scores of the students correlated well with anatomy test scores achieved at the institutional examination (r=0.76). Scores between the other institutions did not show a significant difference. Conclusion: AKRA tool is a validated MCQ-based tool to assess the clinical anatomy knowledge in medical students and doctors.
Extending surjective maps preserving the norm of symmetric Kubo-Ando means
Authors: Chetcuti Emmanuel , Healey Curt 
Published: January 2025

Abstract
In Dong et al. (2022, Journal of Operator Theory 88, 365–406), the authors addressed the question of whether surjective maps preserving the norm of a symmetric Kubo-Ando mean can be extended to Jordan

$\ast $

-isomorphisms. The question was affirmatively answered for surjective maps between the positive definite cones of unital

$C^{*}$

-algebras for certain specific classes of symmetric Kubo-Ando means. Here, we give a comprehensive answer to this question for surjective maps between the positive definite cones of

$AW^{*}$

-algebras preserving the norm of any symmetric Kubo-Ando mean.
Integrated Tourism Model Based on Crafts and Culture Potential to Encourage Quality Tourism
Authors: Hilman Iman , Fachrurozi Miftahul Habib , Armiyati Laely 
Published: January 2025

Objectives: The primary objective of this research is to promote quality tourism to develop regions with rich local culture and craft potential. Specifically, the study aims to create an integrated tourism model that enhances cultural and craft tourism in Tasikmalaya, Indonesia. By focusing on the local heritage, arts, and crafts, the study seeks to identify and leverage these resources for tourism development.

Methods: This study employs qualitative research with a field research approach. The research was conducted at various cultural and craft tourism attractions across Indonesia, particularly in Tasikmalaya. The fieldwork involved identifying and categorizing local tourism attractions to explore the region’s cultural and craft tourism potential.

Results: The research identified 43 potential cultural and craft tourism attractions in Tasikmalaya, which were categorized into four main types of tourism: Heritage tourism, Arts tourism, Creative tourism, and Crafts tourism. These attractions were then grouped into seven distinct clusters, forming an integrated tourism model. This model aimed to bring together different tourism elements for the development of a cohesive and sustainable tourism ecosystem in the region.

Conclusion: The integrated tourism model can be implemented through strategic actions focused on strengthening social capital, promoting cultural preservation, creating a sustainable investment-friendly environment, and developing infrastructure that connects leading tourism attractions. These actions will help enhance the overall tourism experience and ensure sustainable growth for cultural and craft tourism in Tasikmalaya.
Treatment Patterns and Outcomes of Ovarian Cancer in Different Centres of Karachi
Authors: Kerio Perwasha , Haider Ghulam , Ahmed Khalil , Zahoor Saima , Perveen Hina , Hayat Muhammad 
Published: January 2025

Objective: To evaluate the treatment pattern i.e. either upfront surgery followed by adjuvant chemotherapy or upfront neoadjuvant chemotherapy followed by surgery and outcomes of advance ovarian cancer in different hospitals of Karachi.
Study Design: observational study
Place and Duration of Study: Oncology Department, Jinnah Postgraduate Medical Center, Karachi Pakistan, from March 2019-Sep 2020.
Methodology: All females of age 18-70 years with diagnosis of epithelial ovarian cancer (stage III-IV) and who had received chemotherapy or on chemotherapy in upfront or in adjuvant setting from another hospital and came to Jinnah Postgraduate Medical Center in mid of treatment due to exhaustion of financial resources, as ovarian cancer treatment costs high and oncology department Jinnah Postgraduate Medical Center provides free treatment.
Results: Of 170 patient, 119(70%) patients received adjuvant chemotherapy after surgery, while 51(30%) received neoadjuvant chemotherapy followed by surgery. Among 51 patients who received neoadjuvant (upfront) chemotherapy, 30(58.8%) had partial and 13(25.5%) had complete clinical response, whereas 42(82.4%) had partial and 9(17.6%) had complete pathological response. About 38(74.5%) had complete and 13(25.5%) had partial biochemical response. Each patient followed for one and half year to see recurrence rate. Patients who underwent surgery 45(48.4%) had recurrence, 38(40.9%) completed the treatment plan and 10(10.7%) died. In patients who underwent neoadjuvant chemotherapy 12(41.4%) showed recurrence, 11(37.9%) completed the treatment plan and 6(20.7%) died.
Conclusion: To date most of the centers in Karachi following the pattern of upfront surgery followed by adjuvant chemotherapy. More disease recurrences are seen in upfront surgery group....
Experience of Carotid Endarterectomy and its Complications Under Superficial Cervical Block in Conscious Patients in a Tertiary Care Hospital
Authors: Khan Aftab Ahmad , Jamil Muhammad , Khan Khurram Shoaib , Ambreen Shazadi 
Published: January 2025

Objectives: To determine the frequency of post-operative complications of carotid endarterectomy conducted under superficial cervical block.
Study Design: Cross-sectional study.
Place and Duration of Study: Department of Vascular Surgery, Combined Military Hospital, Rawalpindi, Pakistan Sep 2019 to Feb 2021.
Methodology: A total of 70 patients who reported to the Vascular Surgery Department, Combined Military Hospital, Rawalpindi Pakistan with an indication for carotid endarterectomy who met the sample selection criteria were included in this study. All patients underwent carotid endarterectomy and were followed up for subcutaneous and subplatysmal hematoma formation, the development of Neuropraxia, stroke and bleeding which required exploration for 4 weeks. Data was analyzed by SPSS 26.0.
Results: A total number of 10(14.3%) developed complications while 60(85.7%) surgeries were uneventful. Out of the total complications, hematomas accounted for 4(40%) of cases, while Neuropraxia and contralateral stroke accounted for 2(20%) of the cases each. Of the remaining two cases one developed myocardial infarction (MI) treated by cardiologist and discharged 5th postoperative day while the other required exploration for persistent hemorrhage.
Conclusion: Carotid endarterectomy under superficial cervical nerve block is a safe and effective procedure, with good outcomes.
Comparison of Quantitative and Semi-quantitative D-dimer in the Diagnosis of Deep Venous Thrombosis
Authors: Ilyas Sumaira , Akhtar Fahim , Hussain Zahoor , Zafar Zara , Suhail Maymoona , Zahir Saima 
Published: January 2025

Objective: To compare sensitivity along with the specificity of quantitative and semi-quantitative D-dimer tests in patients of Deep venous thrombosis.
Study Design: Cross-sectional study.
Place and Duration of Study: Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from Mar 2021 to Feb 2022.
Methodology: A total of 176 patients were recruited using a nonprobability consecutive sampling technique. Patients with age <60 years and suspected of having deep venous Deep venous thrombosis were included. Patients with age >60 years and who were on anticoagulation therapy or have raised D-dimer due to other causes were excluded. The sample obtained in the trisodium citrate tube was analyzed both by semi-quantitative and quantitative methods. A value above 200ng/ml was considered positive. Sensitivity, specificity, and negative and positive predictive values were measured. Receiver Operating Characteristic was made taking Doppler ultrasound as a gold standard.
Results: In our study males 116(60%) and females 60(34%). Based on the gold standard test, and Doppler ultrasound, 24(14%), patients did not have Deep venous thrombosis while 152(86%) were diagnosed with Deep venous thrombosis. The Quantitative D-dimer test has a sensitivity of 98.7% and a specificity of 73.1%. Whereas sensitivity, specificity, positive and negative predictive value of the semiquantitative test was 92.7%, 100%, 92.1%, and 52.1% respectively. The area under the Receiver Operating Characteristic curve was 0.911 for the semiquantitative test and 0.873 for a quantitative test.
Conclusions: Semiquantitative D-dimer test has acceptable sensitivity and better specificity as compared to quantitative assay using Doppler ultrasound as a gold