bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineering microalgal cell wall-anchored proteins using GP1 PPSPX motifs and releasing with intein-mediated fusion
Authors: Kang Kalisa , do Espirito Santo Évellin , Diaz Crisandra Jade , Mayfield Stephen , Dutra Molino João Vitor 
Published: January 2025

AbstractHarnessing and controlling the localization of recombinant proteins is critical for advancing applications in synthetic biology, industrial biotechnology, and drug delivery. This study explores protein anchoring and controlled release inChlamydomonas reinhardtii, providing innovative tools for these fields. Using truncated variants of the GP1 glycoprotein fused to the plastic-degrading enzyme PHL7, we identified the PPSPX motif as essential for anchoring proteins to the cell wall. Constructs with increased PPSPX content exhibited reduced secretion but improved anchoring, pinpointing the potential anchor-signal sites of GP1 and highlighting the distinct roles of these motifs in protein localization. Building on the anchoring capabilities established with these glycomodules, we also demonstrated a controlled release system using a pH-sensitive intein derived from RecA fromMycobacterium tuberculosis. This intein efficiently cleaved and released PHL7 and mCherry that was fused to GP1 under acidic conditions, enabling precise temporal and environmental control. At pH 5.5, fluorescence kinetics demonstrated significant mCherry release from the pJPW4mCherry construct within 4 hours. In contrast, release was minimal under pH 8.0 conditions and negligible for the pJPW2mCherry (W2) control, irrespective of the pH. Additionally, bands on the Western blot at the expected size of mCherry also showed its efficient release from the mCherry::intein::GP1 fusion protein at pH 5.5. Conversely, at pH 8.0, no bands were detected. This anchor-release approach offers significant potential for drug delivery, biocatalysis, and environmental monitoring applications. By integrating glycomodules and pH-sensitive inteins, this study establishes a versatile framework for optimizing protein localization and release inC. reinhardtii, with broad implications for proteomics, biofilm engineering, and scalable therapeutic delivery systems.Graphical Abstract
Schaffer commissural inputs regulate the stability of CA1 spatial representation
Authors: Rais Cynthia , Maheu Maxime , Wiegert J. Simon 
Published: January 2025

ABSTRACTNeuronal representations of the world are dynamic. A striking example is the rapid remapping of the hippocampal spatial code, which occurs even when the environment and behavior remain unchanged. Here, we tested the possibility that CA3 Schaffer commissural inputs to CA1 control the stability of the cellular place representation in dorsal CA1. By combining longitudinal two-photon calcium imaging of CA1 place cells with optogenetic stimulation of contralateral presynaptic CA3 neurons in mice running on a linear treadmill, we demonstrate that CA3 inputs regulate the CA1 place code. Activation of CA3 cells both induced a small pool of new CA1 place cells and altered the pre-existing CA1 place code. The latter occurred through an unbiased shift of existing place fields specifically, and not through modifications of place field precision or a complete loss of place tuning in pre-existing place cells. All CA3-driven changes in the CA1 allocentric place code were transient and almost completely disappeared by the next day. Taken together, these results confirm a role for Schaffer commissural inputs in regulating CA1-encoded spatial representations, thus possibly contributing to the rapid scaffolding and remapping of spatial memories.
Simulation of the process
e+e−→W+W−
with the heavy right-handed neutrino exchange at 1 TeV future lepton colliders
Authors: Drutskoy A. , Vasenin E. 
Published: January 2025

We study potential contribution of the heavy right-handed neutrino exchange in the process e+e−→W+W−. This process is sensitive to heavy neutrinos with masses larger than s. The Monte Carlo simulation of the studied process is performed assuming the seesaw type-I model, where heavy right-handed neutrinos (heavy neutral leptons, HNLs) are introduced in the leptonic sector. Within the Standard Model (SM), the process has a large cross section described by diagrams with s-channel Z/γ exchange and t-channel active neutrino exchange. Respectively, the t-channel right-handed neutrino exchange amplitude will interfere with these SM amplitudes. However, the angular distributions of the W boson production and decay are different for the right-handed neutrino and SM amplitudes. That can be used to evaluate potential HNL contribution using the extended likelihood method. The simulation of the e+e−→W+W− process is performed at the 1 TeV center-of-mass energy and polarization Pe+e− of (20%, −80%), which is a standard option for the future linear e+e− International Linear Collider. Both W bosons are reconstructed from two hadronic jets. Simulation of the SM background processes is also done. The beam-induced backgrounds and the initial state radiation effects are taken into account. The majority of background processes are effectively suppressed by the cuts on the invariant masses of two and four jets. Finally, we obtain upper limits on the mixing parameter |VeN|2 as a function of M(N).

Published by the American Physical Society
2025
Infrastructure and economic growth: evidence from Zambia
Authors: Sichoongwe Kiru 
Published: January 2025

PurposeThis study aims to empirically assess how infrastructure development influenced economic growth in Zambia from 2000 to 2020.Design/methodology/approachThe study uses data from the World Development Indicators (WDI), spanning from 2000 to 2020. The selection of this time period was determined by the availability of data related to the research. The Autoregressive Distributed Lag (ARDL) bounds testing approach was used for data analysis.FindingsThe findings show that economic growth is cointegrated with capacity to generate electricity, proving the existence of a long-run equilibrium relationship between them. Furthermore, the empirical results established that electricity generation capacity had a positive and significant impact on economic growth. Similarly, in the short run, electricity generation capacity, and mobile cellular services had a positive impact on economic growth.Practical implicationsPolicy measures should prioritise increasing capacity for producing electricity and expanding access to energy by relevant economic sectors. Increased access to energy by these sectors can raise productivity, spur economic growth and accelerate industrialisation. Also, in the light of climate change, it is crucial that policymakers explore alternate sources of electricity generation, such as green and renewable sources. Furthermore, policy initiatives should prioritise expanding mobile cellular infrastructure, given that mobile cellular technology has become a vital component of economies and continues to offer unprecedented opportunities for economic growth.Originality/valueThis study presents novel empirical evidence on the unique relationship between infrastructure and economic growth in Zambia, highlighting electricity generation and mobile cellular services as pivotal factors for enhancing productivity and spurring industrial development.
Where has the time gone? A configurational analysis of the utilization level of China’s municipal open government data (OGD) portals to reclaim “time”
Authors: Wu Yingqiang , Huang Ruhua , Li Baiyang 
Published: January 2025

Purpose
This study aims to overcome the “time blind spot” by exploring the multifactorial synergistic effects that enhance the utilization levels of open government data (OGD) portals over time. The objective is to provide theoretical contributions and practical value for research related to open government data.

Design/methodology/approach
This study applies the dynamic qualitative comparative analysis (QCA) method to analyze panel data from 29 municipal portals spanning 2019–2023. Specifically, it examines the trends in the utilization levels of China’s OGD portals over time and identifies configurations of conditions that consider both spatial and temporal factors.

Findings
There are no necessary but insufficient conditions affecting the utilization levels of OGD portals. Instead, rather than being controlled by a single factor, the utilization levels are influenced by the combined effect of multiple factors. A sufficiency analysis of condition combinations identifies three configuration paths: portal and data resource development driven by fiscal support, portal development and public demand driven by government leadership and portal and data resource development driven by government leadership. In particular, the level of data portal development is identified as a core factor in achieving high utilization levels of OGD portals. Furthermore, while the configuration analysis results do not show significant temporal effects, there are case effects caused by a certain degree of spatial differences.

Originality/value
This pioneering study combines the dynamic QCA method based on panel data with the theme of OGD. Using panel data from 29 municipal platforms over the period from 2019 to 2023, this research addresses the shortcomings of limited time frames that have been encountered in previous studies. Moreover, it explores the configurational paths to enhancing platform utilization over time, aiming to provide both theoretical contributions and practical value for research related to the utilization of OGD portals.
The impact of IFRS adoption on the relevance of financial reporting in emerging markets: the case of Moroccan listed companies
Authors: OUBAHOU Yassine , EL OUAFA Khalid , BENGRICH Mustapha 
Published: January 2025

PurposeThis study aims to assess the impact of the introduction of International Financial Reporting Standards (IFRS) on the informational relevance of accounting figures for Moroccan companies listed on the Casablanca Stock Exchange. Specifically, it seeks to determine whether IFRS adoption improves the ability of accounting data to reflect share price movements.Design/methodology/approachThe empirical analysis covers a sample of 27 Moroccan listed companies over a six-year period (2015–2020), with a total of 162 observations. The study tests various hypotheses through association studies to evaluate the effectiveness of accounting figures in reflecting share prices in the pre-IFRS and post-IFRS periods. The analysis utilizes Ohlson's (1995) price model, which is widely used in the literature for such assessments.FindingsThe results show that both earnings and shareholders’ equity are more effective in explaining share price variations in the post-IFRS period than in the pre-IFRS period. In particular, equity demonstrates greater explanatory power than earnings. The combined explanatory power of equity and earnings is significantly stronger in the post-IFRS period, suggesting that the adoption of IFRS has played a key role in enhancing the quality of financial information disclosed in the Moroccan market.Practical implicationsTheoretical implications – this study highlights that the adoption of IFRS reduces information asymmetry, thereby mitigating conflicts of interest and agency costs. By aligning accounting practices with international standards, IFRS enhance transparency and governance, fostering a more reliable business environment. Practical implications – the study indicates that IFRS adoption improves the transparency, reliability and comparability of financial information, enhancing investor confidence and making the Moroccan financial market more attractive. It provides useful guidance for regulators, businesses and countries considering IFRS implementation, demonstrating the importance of these standards in boosting market transparency and attracting global investments.Originality/valueThis study explores the impact of IFRS adoption on the relevance of financial information in Morocco, a topic that has been underexplored in emerging economies. It fills a gap in the literature by providing insights into IFRS adoption in a specific context, thus contributing to the ongoing debate on their effectiveness in developing economies.
Does eWoM matter in s-commerce? A comparatives study between Kuwait and United Arab Emirates
Authors: Abbas Hasan A. , Rouibah Kamel , Al-Qirim Nabeel 
Published: January 2025

Purpose
This study aims to explore the antecedent factors that directly and indirectly influence electronic word of mouth (eWoM) for social commerce (s-commerce) in two developing countries (e.g. Kuwait and the United Arab Emirates [UAE]) by extending social cognitive theory.

Design/methodology/approach
This study uses a previous robust model (Rouibah et al., 2021) as theoretical background to investigate and compares the antecedents (trust in Instagram, perceived risks) on eWoM for s-commerce through the mediation of three mediators (perceived enjoyment, perceived value and customer satisfaction) among two Arab countries. Data was collected from Kuwait (n = 1,132) and the UAE (n = 190). Different statistical analyses and structured equation modeling-based analysis of moment structure are used to test the robustness of the research model.

Findings
This study found customer satisfaction to be most important factor that mediates the effect independent factors on eWoM for s-commerce in both countries. Surprisingly, perceived enjoyment has no effect, and trust in Instagram and perceived risks are the most important factors that are considered imperative for customer satisfaction and positive feedback.

Research limitations/implications
One limitation of this study is that the author does not focus on the difference between the effects of textual and graphical information on customers’ decisions and trust in buying merchandise. Another limitation is that this study focuses on Kuwait and the UAE. Other Gulf Cooperation Council countries are also growing exponentially, and mobile and internet penetration rates are booming; they could be a trigger for more studies on whether differences occur among all of them.

Practical implications
The first implication is that it is the first in its field to extend the effects of eWoM. To the best of the author’s knowledge, compared to the online research this study is unique because the authors examine six factors for eWoM in s-commerce using the Instagram platform as opposed to other platforms.

Social implications
The third implication of this study is that the previous ones have applied eWoM to different subjects of e-commerce such as tourism and marketing but have concentrated less on s-commerce, where in-depth research is needed much more to explore factors and theories that explain human behavior.

Originality/value
Furthermore, most of these studies have focused on the intention to use (Dincer and Dincer, 2023; X. Hu, Chen, Davison, and Liu, 2022; Zhou et al., 2023). However, the attention in this research is on the actual use.
Appraising young adult built environment researchers training needs in a developing economy: a perspective from stakeholders
Authors: Ebekozien Andrew , Aigbavboa Clinton , Ahmed Mohamed Ahmed Hafez , Samsurijan Mohamad Shaharudin , Aliu John Ogbeleakhu , Ikuabe Matthew , Nwaole Angeline Ngozika Chibuike 
Published: January 2025

PurposeStudies have shown that research is a global innovation’s “engine room.” Therefore, young adult-built environment researchers (YABER), especially in developing countries, need research upskilling and reskilling training for better-integrated outcomes. Evidence shows that research training can improve YABER competencies and proficiencies. In Nigeria, YABER may have had some challenges in training needs. There is a lack of extant literature about the issues facing Nigeria’s YABER upskilling and reskilling training needs. Thus, this study investigated the perceived problems facing YABER and proffered measures to improve their training needs in Nigeria.Design/methodology/approachThe researchers used participants from Edo State, Abuja and Lagos State, Nigeria. This study adopted a qualitative research approach. The researchers accomplished saturation after 40 virtual interviews and adopted a thematic analysis for the primary data.FindingsThe research shows that the built environment research refines current and creates new knowledge. The built environment researchers (trainers and trainees) need training but face challenges. Findings clustered measures to improve quality research publications in the built environment through YABER training into government/regulatory agencies-related, higher education institutions-related and researcher-related measures.Originality/valueThe developed framework and thematic network analysis could be used to stimulate YABER training needs and, by extension, stir Nigeria’s higher education institutions regulatory agencies to upgrade requirements for academic staffers, especially published articles, to be compulsorily Scopus/Web of Sciences indexed for promotion exercise. This is the global best practice, and Nigerian scholars cannot be in isolation. It will form part of this study’s implication and revitalise UNSDG 4.
The challenges clinical supervisors experience when supervising students in the workplace
Authors: Gustavsson Maria , Bivall Ann-Charlotte 
Published: January 2025

PurposeThis article aims to provide a deeper understanding of the challenges clinical supervisors’ experience when supervising students’ professional learning in the workplace.Design/methodology/approachQualitative interviews were conducted with 13 healthcare professionals, who are experienced clinical supervisors. They were employed either in a hospital ward or in one of two municipal care units.FindingsThe findings showed that clinical supervisors must navigate a complex landscape of challenges to ensure students’ professional learning. They had to balance their regular duties with the time and effort required for supervision. Prominent challenges were ensuring students met their learning objectives and determining when to allow students to handle tasks independently. The supervisors also faced challenges that were beyond their control. Their workplace conditions were not designed to accommodate students effectively. They struggled with negative attitudes from colleagues towards student supervision and they wanted the clinic managers to be more involved. The gap between the university’s educational demands and the realities of the workplace also presented supervisors with a significant challenge.Originality/valueApart from unique empirical material depicting clinical supervisors’ experiences of student supervision, the value of this study lies in the recommendation that supervisors have clear requirements from higher educational institutions, but of equal importance is that these requirements align with the conditions in the workplace. Further studies are necessary to understand the role that workplace conditions play for clinical supervisors in creating supportive learning environments that facilitate students in their journey to becoming established professionals.
Maternal and paternal influence on female entrepreneurial identity: a narrative analysis
Authors: Sarfati Gilberto , Ulreich Constanze 
Published: January 2025

PurposeThis study explores the role of maternal and paternal influence in shaping the entrepreneurial identities and motivations of female founders. It seeks to fill the gap in the literature by examining how different forms of parental influence affect female entrepreneurs.Design/methodology/approachThis research uses a narrative analysis approach to examine the life stories of 22 female entrepreneurs from Germany, Switzerland, Canada, and Brazil. Through in-depth interviews, the study identifies key themes that illustrate the impact of parental influence on entrepreneurial identity formation.FindingsThe analysis identifies seven distinct themes: “Mom’s Dream,” “Absence,” “Equality First,” “Never Enough,” “Overprotected,” “Atypical Role Model,” and “The Rock.” These themes highlight the complex interplay between family dynamics and entrepreneurial motivations, demonstrating how parental roles and attitudes significantly contribute to forming entrepreneurial identity.Research limitations/implicationsThe study’s findings are based on a relatively small sample size from four countries, which may limit the generalizability of the results. Future research could expand the sample and explore diverse cultural contexts to enhance understanding. Longitudinal studies could also provide deeper insights into the evolving impact of parental influence on entrepreneurial identity over time.Practical implicationsThe findings offer valuable insights for designing targeted support programs for female entrepreneurs. Mentorship programs can address gaps in parental support, while entrepreneurship education can highlight the interplay of maternal and paternal influences in fostering confidence and resilience. Encouraging parents to support their daughters’ entrepreneurial ambitions can help build confidence and resilience in future female entrepreneurs, contributing to a more inclusive entrepreneurial ecosystem.Originality/valueThis study advances identity theory in entrepreneurship by integrating family dynamics into understanding entrepreneurial identity formation. It highlights the dual influences of mothers and fathers and their complementary contributions to resilience, confidence, and ambition. The research also provides a nuanced perspective on gender dynamics within entrepreneurship, emphasizing the importance of family context.
New technologies in HR: bridging efficiency and ethical considerations
Authors: Sposato Martin , Dittmar Eduardo Carlos , Vargas Portillo Jenny Patricia 
Published: January 2025

Purpose
This systematic literature review examines the impact of Web-based human resource management (HRM) systems on organizational practices, exploring both the benefits and challenges associated with their implementation. This study aims to provide a comprehensive understanding of how these technologies transform HR processes while addressing ethical concerns and implications for employee well-being and engagement.

Design/methodology/approach
This study uses a systematic literature review methodology, analysing peer-reviewed academic journals, industry reports and relevant books published between 2000 and 2023. The research uses a tripartite framework to explore the positive aspects (“The Good”), challenges and limitations (“The Bad”) and ethical considerations (“The Ugly”) of Web-based HRM systems, with particular attention to employee engagement and organizational effectiveness.

Findings
This study reveals that Web-based HR systems significantly enhance efficiency in recruitment, compensation and payroll management. However, they also present substantial challenges related to data security, user competence and system integration. Ethical concerns emerge regarding privacy, potential biases in artificial intelligence-driven decision-making and the risk of over-dependency on technology. The research underscores the need for a balanced approach in implementing these systems, considering both their transformative potential and impact on employee well-being.

Originality/value
This study contributes to the existing literature by providing a nuanced, holistic examination of Web-based HRM systems. It extends the theoretical landscape by addressing the darker sides of HR technology adoption, including ethical dilemmas and potential negative impacts on employee engagement. The research offers valuable insights for both academics and practitioners, providing a foundation for future research and practical recommendations for effective implementation.