bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on the Mechanism of Duhuo in Treating Intervertebral Disc Degeneration based on Bioinformatics and Network Pharmacology
Authors: Liu Fei , Xu Zhiwei , Zhang Chi , Yang Lei , Li Zhifa , Wu Xiaofei , Jiang Chen , Liu Zongchao , Chen Feng , Yan Qian 
Published: October 2023

Abstract
Background
Intervertebral disc degeneration (IVDD) is a common orthopedic disease in middle-aged and elderly people, and the low back pain caused by it brings a heavy burden on the economy and life of patients. The study found that the apoptosis and autophagy of nucleus pulposus cells mediated by BCL2, CASP3, CASP8, CASP9, etc. are closely related to IVDD. In addition, studies have found that Duhuo has the effect of anti-inflammation and improving intervertebral disc degeneration, but the specific molecular mechanism is still unclear.
Methods
In this study, we used bioinformatics methods to discover the apoptosis and autophagy molecular mechanisms of IVDD, and further discovered the apoptosis and autophagy mechanisms of Duhuo in treating intervertebral disc degeneration through network pharmacology.
Results
Through bioinformatics analysis, we found that NLRP3, IL6, A KT1, M MP9 and other inflammatory factors are highly expressed in patients with IVDD. The main cellular processes involved are senescence, apoptosis, autophagy, and ferroptosis. The key signaling pathways are HIF − 1, FoXo, PI3k-Ak and other signaling pathways, the key immune cells are T cells and Th 17 cells. Network pharmacology found that Ammidin, isoimperatorin, beta-sitosterol, O- acetylcolumbianetin, and Angelol D, the main active ingredients of Duhuo, may play an important role. There are 23 potential targets for the treatment of IVDD by living alone, among which BCL, CASP3, CASP8, CASP9, ERR1 and PTGS1 may play an important role. Enrichment analysis showed that Duhuo mainly improves intervertebral disc degeneration by regulating apoptosis, autophagy, HIF − 1, and PI3k -Akt signaling pathways. Molecular docking results showed that beta-sitosterol, the main active ingredient of Duhuo, can interact with key targets. Effective combination further confirms our results.
Conclusion
In summary, we have revealed the molecular mechanism of intervertebral disc degeneration, and further discovered the intrinsic mechanism by which Duhuo regulates nucleus pulposus cell apoptosis and autophagy to improve IVDD.
Maternal obesity, interpregnancy weight changes and congenital heart defects in the offspring: a nationwide cohort study
Authors: Hedermann Gitte , Thagaard Ida , Hedley Paula , Krebs Lone , Hagen Christian , Sorensen Thorkild , Christiansen Michael , Ekelund Charlotte 
Published: October 2023

Abstract

Objective To evaluate the association between maternal BMI and congenital heart defects (CHDs) in the offspring when including live births, stillbirths, aborted and terminated pregnancies and to investigate if maternal interpregnancy weight changes between the first and second pregnancy influences risk of foetal CHDs.Methods A nationwide cohort study of all singleton pregnancies in Denmark from 2008 to 2018. Data were retrieved from the Danish Foetal Medicine Database, which included both pre- and postnatal diagnoses of CHDs. Children or foetuses with chromosomal aberrations were excluded. Relative risks were calculated using log-linear Poisson models for CHDs overall, severe CHDs and for five of the most prevalent subtypes of CHDs.Results Of the 547 178 pregnancies included in the cohort, 5 498 had CHDs (1.0%). Risk of CHDs became gradually higher with higher maternal BMI; for BMI 30-34.9 kg/m2, adjusted relative risk (aRR) 1.23 (95% CI 1.12–1.36), for BMI 35-39.9 kg/m2, aRR 1.26 (95% CI 1.09–1.46) and for BMI ≥ 40 kg/m2, aRR 1.81 (95% CI 1.50–2.15). Data was adjusted for maternal age, smoking status and year of estimated due date. The same pattern was seen for the subgroup of severe CHDs. Among the atrioventricular septal defects (n = 245), a particularly strong association with maternal BMI ≥ 40 kg/m2 was seen, aRR 4.19 (95% CI 2.13–7.42). 107 627 women were identified with their first and second pregnancies in the cohort. Interpregnancy BMI change was associated, albeit not statistically significant, with risk of CHDs in the second pregnancy when adjusting for maternal age and BMI, with an aRR 1.27 (95% CI 0.96–1.64) among persons with a BMI increase of ≥ 4 kg/m2.Conclusion Risk of foetal CHDs became gradually higher with higher maternal BMI, but only a non-significant association was seen for interpregnancy weight changes and risk of CHDs.
Automated Conflict Detection in Software Functional Requirements using Rule-Based Natural Language Processing
Authors: Malik Urooj Ali , Bangash Yawar Abbas , Iqbal Waseem , Zhenisbekovna Shynar Mussiraliyeva , Hammad Muhammad 
Published: October 2023

Abstract
The requirement phase stands as the keystone of the software development process , establishing the bedrock upon which successful software projects are built. This paper underscores the critical significance of the requirement phase and the timely resolution of inconsistencies within it. Accurate and complete requirement gathering forms the linchpin of software quality and functionality, making it pivotal for project success. However, manual identification of conflicts and inconsistencies in requirements can be a formidable task, often elusive due to their subtlety and concealed nature. The ramifications of unresolved inconsistencies in software requirements can lead to chaos in later stages of development, necessitating costly and time-consuming 1 revisions. To circumvent these challenges, there is a pressing need for automated conflict detection mechanisms in software requirements. In this research, we present a novel automated approach based on rule-based techniques to detect redundant and conflicting requirements. Our methodology is structured into multiple layers, commencing with the identification of key elements such as actors, actions, Action Negativity, events, event negativity, and restrictions within software requirements. To accomplish this, we harness the power of the CoreNLP library and implement specific Natural Language Processing (NLP) rules. Once these elements are discerned, we employ predefined rules to flag conflicts. To validate the efficacy of our approach, we conducted extensive testing utilizing real-world datasets, including WorldVista and Pure. Our results showcase a remarkable performance, with an average precision rate of 92%, a recall rate of 94.5%, and an impressive F1-score of 93% for both the WorldVista and Pure datasets. This research paves the way for enhanced software requirement analysis and lays the foundation for more robust and error-free software development processes.
Airborne imagery does not preclude detectability issues in estimating bird colony size
Authors: Couturier Thibaut , Gaillard Laurie , Vadier Almodis , Dautrey Emilien , Mathey Jérôme , Besnard Aurélien 
Published: October 2023

Abstract
Aerial images obtained by drones are increasingly used for ecological research such as wildlife monitoring. Yet detectability issues resulting from animal activity or visibility are rarely considered, although these may lead to biased population size and trend estimates. In this study, we investigated detectability in a census of Malagasy pond heron Ardeola idae colonies on the island of Mayotte. We conducted repeated drone flights over breeding colonies in mangrove habitats during two breeding seasons. We then identified individuals and nests in the images and fitted closed capture-recapture models on nest-detection histories. We observed seasonal variation in the relative abundance of individuals, and intra-daily variation in the relative abundance of immature birds, affecting the availability of nests for detection. The detection probability of nests estimated by capture-recapture varied between 0.58 and 0.74 depending on flyover days and decreased 25% between early and late morning. A simulation showed that three flyovers are necessary to detect a 5–6% decline in colonies of 50 to 200 nests. These results indicate that the detectability of nests of forest-canopy breeding species from airborne imagery can vary over space and time; we recommend the use of capture-recapture methods to control for this bias.
Prediction of a lncRNA-miRNA co-expression network as a prognostic marker in glioblastoma
Authors: Liu Zhengzheng , Wang Min , Kuang Weilu 
Published: October 2023

Abstract

Glioblastoma has the characteristics of high proliferation and high metastasis; it has become one of the most difficult tumors to treat in clinic. Recently, more and more attention has been paid to the role of competitive endogenous RNA (cyclin-inhibitor receptor, CeRNA) in cancer. However, there is still limited research on the role of endogenous RNA interactions based on high-throughput sequencing data in the occurrence and development of glioblastoma. In this study, RNA-seq data (miRNA expression profile and lncRNA expression profile) and clinical follow-up data (age, survival time, etc) of human brain low-grade glioblastomas (LGG) were obtained from The Cancer Genome Atlas (TCGA) database. In addition, CIBERSORT was applied to assess immune infiltrates and microenvironmental indicators in LGG. The similarities between the mRNA modules with clinical traits were subjected to weighted correlation network analysis (WGCNA). Based on the mRNAs from clinical-related modules, a survival model was established by univariate and multivariate Cox proportional hazard regression analyses. In the survival model, 519 glioblastoma patients were divided into high-risk (N = 259) and low-risk (N = 260) groups and this model predicts one-year survival in LGG patients, with an AUC of 0.88. Then, we predicted interactions between lncRNAs and miRNAs by starBase. Finally, we identified lncRNA PSMA3-AS1 and miR-143-3p for the construction of a ceRNA network, which might play a key role in the development of glioblastoma and act as a prognostic biomarker of glioblastoma. In this study, we hypothesize that this ceRNA network will impact the immune activity and tumor microenvironment changes in glioblastoma. Therefore, this study provides a new direction for investigating the development and treatment of glioblastoma.
Test Anxiety Among Health Profession Students: A Cross-Sectional Survey at Fatima College of Health Sciences in Al Ain, United Arab Emirates
Authors: El-Sobkey Salwa B. , Banu Shaik Balkhis , Hammoud Emne , Salih Suliman , Sachdeva Monika , Kamil Noon , Aldawsari Aisha Namshan , Cottam Scott 
Published: October 2023

Abstract
Background: Test anxiety (TA) affects students before and/or during exams and has become an incremental worldwide phenomenon.
Objectives: This study aimed to measure the TA intensity for health professionstudents (HPSs) at the Fatima College of Health Sciences (FCHS) at Al Ain Campus, UAE, and to recognize demographic and academic correlative and/or associated factors.
Methods: A three-section questionnaire was used to collect data including the HPSs’ demographic and academic characteristics as well as to collect their response to the achievement anxiety test (AAT). This AAT assesses the intensity of TA and determines two independent subscales of debilitating and facilitating patterns of this TA. The HPSs were recruited from five departments at the FCHS to voluntarily participate in the study.
Results: The number of students who participated in the study was 215 from all academic levels. The results revealed that the mean totalAAT score was 57.2 points (60.2%), with no significant difference among departments; the debilitating independent subscale had 31.9%, and the facilitating independent subscale had 28.3%. All the studied academic factors and two of the studied personal factors showed a significant correlation withAAT scores.
Interpretation: The HPSs at FCHS, Al-Ain Campus had a moderate TA intensity level, and almost half of this TA is of the good facilitating type that encourages and motivates students to study and prepare well for their exams. Senior students with high college entry grades, high GPA in the previous semester, high CGPA, a lower average course load per semester, living in a villa, and having family social support had significantly lower AAT scores.
Conclusions: The HPSs at FCHS have a comparatively lower TA intensity than other health colleges in the UAE and Gulf region. Different academic and demographic factors showed a significant correlation with AAT scores.
Developing Implementation Strategies for Promoting Integrative Oncology Outpatient Service Delivery and Utilisation: A Qualitative Study in Hong Kong
Authors: Ho Leonard , Kwong Ming Hong , Li Angus SC , Nilsen Per , Ho Fai Fai , Zhong Claire CW , Wong Charlene HL , Ling Lin Wai , Chung Vincent CH 
Published: October 2023

Abstract

Purpose:Current evidence supports the use of integrative oncology (IO) interventions in cancer supportive care. The demand for outpatient IO services in Hong Kong is expected to soar following the surge in cancer incidence in an ageing population. This study identified the implementation determinants of delivering and utilising outpatient IO from local stakeholders’ perspectives and developed corresponding implementation strategies.Methods:This study involved two sequential stages. First, with individual semi-structured interviews guided by the Theoretical Domains Framework (TDF), we explored stakeholders’ views on the barriers to and facilitators for implementing IO. Second, guided by a TDF-based qualitative data analysis of interview transcripts, we performed intervention mapping to develop Behaviour Change Wheel-based implementation strategies that may overcome the barriers and strengthen the facilitators.Results:We interviewed 31 stakeholders, including traditional Chinese medicine (TCM) practitioners (n=8), biomedically-trained doctors (n=7), nurses (n=6), administrators (n=4), caregivers (n=4), and pharmacists (n=2). The common local implementation determinants of IO are (1) lacking nursing and administrative manpower supporting IO service delivery, (2) lacking awareness of IO services among healthcare professionals, administrators, patients, and caregivers, and (3) lacking knowledge among healthcare professionals of herb–drug interaction and herbal toxicities.Conclusion:We recommended a multi-faceted implementation strategies package that included arranging additional funding to train, recruit, and retain experienced nursing and administrative staff, devolving resources into demonstrating successful interprofessional collaborations and clinical evidence on IO effectiveness and safety, integrating evidence on herb–drug interactions and herbal toxicities into automated electronic health record systems monitored by pharmacists with dual qualifications in TCM and conventional pharmacy.
Fuzheng Kang-Ai decoction increases the sensitivity of Gefitinib via inducing Non-Small Cell Lung Cancer ferroptosis through AMPK/SLC7A11 pathway
Authors: Zhao YueYang , Xu MengFei , Wang ShuJing , Zhong NanXing , Qiu ShengHong , Tang Qing , Zhou QiChun , Pang YanQing , Lin HuanMei , Yang XiaoBing , Wu WanYin , Wang SuMei 
Published: October 2023

Abstract
Background:
Fuzheng Kang-Ai decoction (FZKA), which consists of twelve Chinese herbal medicines (CHM), has been extensively used in the treatment of Non-Small Cell Lung Cancer (NSCLC) patients. It was reported in our preceding study that the progression-free survival (PFS) could be prolonged and the toxic effect was reduced after the combination of FZKA and Gefitinib (GFTN) comparing to GFTN alone. Recent studies have shown that FZKA could promote ferroptosis in NSCLC cells. Hence, we assumed that FZKA might strengthen the effect of GFTN by regulating cell ferroptosis in NSCLC.
Results:
First of all, according to our results, FZKA increased the inhibition effect of NSCLC by GFTN in vitro. Next, we found that FZKA increased the sensitivity of GFTN on cell ferroptosis by testing lipid peroxidation and intracellular-free iron. Interestingly, the effect of FZKA on increasing sensitivity of GFTN in NSCLC cells could be reversed by blocking ferroptosis. Additionally, the results demonstrated that the expression of AMPKα was increased and SLC7A11 reduced in the combined treatment group, at both mRNA and protein levels. Notably, the inhibition of AMPKα reversed the effect of FZKA on increasing GFTN sensitivity. Finally, in vivoexperiments validated that FZKA strengthened the effect of GFTN by inducing NSCLC cell ferroptosis.
Conclusions:
Based on our findings, we conclude that FZKA increases the sensitivity of GFTN via inducing NSCLC ferroptosis through AMPK/SLC7A11 pathway, providing the solid evidences to the treatment of FZKA together with GFTN in NSCLC patients.
Explanations from ChatGPT: Targeted Task Improvements But No Generalized Gains in Metacognitive Abilities for Novice Language Learners
Authors: Mousazadeh Mohammad 
Published: October 2023

Abstract
This study investigated the potential benefits of ChatGPT’s explainability for enhancing self-regulated learning in novice language learners. Specifically, explanations from ChatGPT were explored as a tool to improve metacognitive awareness and task performance. 50 intermediate Iranian English learners engaged with either an explainable version of ChatGPT that justified its feedback or a non-explainable version without elucidations. Quantitative results showed no significant differences between groups on standardized measures of metacognitive knowledge or writing performance, indicating explainable ChatGPT did not improve these skills. However, the explainable ChatGPT group demonstrated significantly higher accuracy on target tasks covered in the system’s explanations. These mixed findings suggest explainable ChatGPT may strengthen understanding of specific content explained but do not confer generalized gains in metacognitive abilities or standardized outcomes. While explainable ChatGPT shows promise for supporting self-regulated learning, determining optimal applications to boost broad learner competencies remains an open research challenge. Further inquiry with larger diverse samples is warranted to fully ascertain the pedagogical potential of explainable ChatGPT in education.
The Millettia speciosa Champ polysaccharide attenuates pyroptosis by suppressing the signaling pathway of caspase-1/gasdermin D/Interleukin-1β
Authors: Han Mengmeng , Wu Yuhui , Duo Shuiyin , Chen Yun , Yang Yuhui 
Published: October 2023

Abstract
Pyroptosis is a novel type of programmed cell death associated with the pathogenesis of many inflammatory diseases. Studies have shown that the Millettia speciosa Champ polysaccharide(MSP)has anti-inflammatory effects, but there is no report on whether the MSP has an effect on pyroptosis from the anti-inflammatory mechanism. This study aims to investigate the inhibitory effects of a water-soluble polysaccharide was obtained from Millettia Speciosa Champ(MSP-1)on the pyroptosis of RAW264.7 cells induced bylipopolysaccharide(LPS)/adenosine triphosphate(ATP). The results showed that MSP-1 significantly inhibited the pyroptosis of RAW264.7 macrophages. MSP-1 inhibited the secretions of IL-6, IL-1β, IL-18, nitric oxide (NO), and lactic dehydrogenase(LDH)and reduced caspase-1, cleaved IL-1β, and cleaved gasdermin D(GSDMD) activation of the cleaved protein levels. MSP-1 ameliorated the scorching of RAW264.7 macrophages by a mechanism that may be related to the downregulation of each of the caspase-1/IL-1β/GSDMD signaling pathways. In addition, the structure of MSP-1 was characterized in this study. MSP-1 consisted of only glucose with an average molecular weight of 50.2 kDa. Moreover, the infrared spectroscopy, methylation, and nuclear magnetic resonance (NMR) results showed that MSP-1 consisted of →4)-α-D-Glcp-(1→, →4, 6)-α-D-Glcp-(1→and T-α-Glcp.
Analysis of perioperative outcomes for esophageal cancer: comparison of robotic-assisted and video-assisted minimally invasive esophagectomy
Authors: He Li , He Hua , Zhao Mengmeng , Sun Shuo , Yang Wenwen , Wang Feng , Zhang Min , Lin Ruijiang , Ma Minjie , Han Biao 
Published: October 2023

Abstract
Objective
Comparing the perioperative outcomes of single-thorax robotic-assisted McKeown minimally invasive esophagectomy (RAMIE) and video-assisted McKeown minimally invasive esophagectomy (VAMIE) in a balanced population.
Methods
From June 2017 to May 2022, 193 esophageal cancer patients were retrospectively included, including 75 cases receiving RAMIE and 118 receiving VAMIE. The balanced population was generated by propensity-score matching, and statistical analyses were conducted to compare patients' demographics and perioperative outcomes in these two groups.
Results
A total of 57 RAMIE cases were matched with 57 VAMIE cases by propensity score. Although the total cost of the RAMIE group after matching was higher than that of the VAMIE group (118.28k vs 136.79k, P = 0.027), there were no significant differences in the number of chest LNs, postoperative drainage and postoperative cardiopulmonary complications. When compared to the VAMIE group, the RAMIE group after matching had shorter surgery time (total 335.00 vs. 235.00 min, P < 0.001; chest 244.00 vs. 139.00 min, P < 0.001), less intraoperative blood loss (200.00 vs. 100.00 ml, P < 0.001), less intraoperative rehydration (2400.00 vs. 1500.00 ml, P < 0.001), lower postoperative VAS (P < 0.001), fewer postoperative hospitalization days (20.00 vs. 14.00 days, P < 0.001), and lower incidence of anastomotic leak (8 [14.04%] vs. 2 [3.51%], P = 0.047).
Conclusion
Compared with VAMIE, the single-thorax RAMIE has shorter surgery time, less intraoperative blood loss/rehydration, less postoperative pain, and a lower incidence of anastomotic leak.
Effects of immunorelated gene polymorphisms on trastuzumab targeting breast cancer cell
Authors: yu lin yu , Zhang Cong-Min , Liu Liang-Yu , Chen Xiao-Ping 
Published: October 2023

Abstract
Purpose Transtuzumab-based immunotherapy has been the standard of care for HER2/neu-expressing breast cancer (BC). However, only 20–30% of patients overexpressing HER2 respond to trastuzumab. This study aimed to investigate the associations between genetic polymorphisms in immunorelated genes and PBMC-induced cytotoxicity to breast cancer cell with the treatment of trastuzumab.Methods Trastuzumab-mediated cytotoxicity of peripheral blood mononuclear cells (PBMC) from 148 healthy donors and 13 BC patients was analyzed by flow cytometry. 16 SNPs in 7 genes (CD247, ZAP70, FCGR2A, FCGR2C, FCGR3C, TNF, and IFNG) were genotyped using the Sequenom Mass Array Genotype Platform. Levels of cytokines were measured with the Th1/Th2Cytokine Kit II.Results Both cytotoxicity and TNF release in the trastuzumab treated PBMCs were significantly higher than those of the basal group, and trastuzumab stimulated cytotoxicity of PBMCs in BC patients as effectively as in healthy donors. A wide variability in trastuzumab-mediated cytotoxicity in 148 healthy donors was observed, and PBMCs from individuals with the CD247 rs16859030 T genotype generated increased cytotoxicity than those with the CD247 rs16859030 CC genotype. We also observed that natural killer cells (NKs) elicited stronger cytotoxicity than PBMCs.Conclusion The CD247 rs16859030 polymorphism affects trastuzumab-mediated cytotoxicity in vitro. Furthermore, trastuzumab-mediated cytotoxicity is not changed in BC patients.