bluuebunny/crossref_metadata_2025_split huggingface dataset:
Concordance and Clinical Significance of Genomic Alterations in Progressive Tumor Tissue and Matched Circulating Tumor DNA in Aggressive-variant Prostate Cancer
Authors: Wang Ruiliang , Xu Qiufan , Guo Hanxu , Yang Guanjie , Zhang Jun , Wang Hong , Xu Tianyuan , Guo Changcheng , Yuan Jing , He Yanyan , Zhang Xiaoying , Fu Hongliang , Xu Guang , Zhao Binghui , Xie Jun , Zhao Tingting , Huang Longfei , Zhang Jiansheng , Peng Bo , Yao Xudong , Yang Bin 
Published: October 2023

Abstract

Sequencing of circulating tumor DNA (ctDNA) is a minimally invasive approach to reveal the genomic alterations of cancer; however, its comparison with sequencing of tumor tissue has not been well documented in real-world patients with aggressive-variant prostate cancer (AVPC). Concordance of genomic alterations was assessed between progressive tumor tissue and matched ctDNA by next-generation sequencing for 63 patients with AVPC. Associations of genomic alterations with progression-free survival (PFS) and overall survival (OS) were investigated using Kaplan–Meier and Cox regression analyses. A total of 161 somatic mutations (SMs) and 84 copy-number variants (CNVs) were detected in tumors, of which 97 were also found in ctDNA, giving concordance of 39.6% (97/245) across all SMs and CNVs, 49.7% for SMs only and 20.2% for CNVs only. Across all patients with AVPC, chemotherapy was associated with significantly longer median PFS (6 vs. 0.75 months, P = 0.001) and OS (11 vs. 8 months, P &lt; 0.001) than next-generation hormonal therapy (NHT). Among types of chemotherapy, additional platinum-based chemotherapy was associated with significantly longer median PFS and OS than docetaxel only in patients with TP53, RB1, or PTEN alterations, and in those with ctDNA% ≥ 13.5%. The concordance analysis first provides evidence for combining the sequencing of ctDNA and tumor tissue in real-world patients with AVPC. Chemotherapy is associated with significantly better survival than NHT, and the benefit of additional platinum-based chemotherapy may depend on the presence of alterations in TP53, RB1, or PTEN and on a sufficiently high proportion of ctDNA in patients with AVPC.

Significance:
AVPC is a highly malignant and heterogeneous disease. Sequencing of ctDNA is a minimally invasive approach to reveal genomic alterations. On the basis of the current real-world study, we found ctDNA does not fully recapitulate the landscape of genomic alterations from progressive tumor tissue in AVPC. We also revealed AVPC can benefit from chemotherapy, especially platinum-based regimens. TP53/RB1/PTEN alterations in ctDNA or tumor tissue could be biomarkers for platinum-based chemotherapy in this setting.
Portfolio Diet Score and Risk of Cardiovascular Disease: Findings From 3 Prospective Cohort Studies
Authors: Glenn Andrea J. , Guasch-Ferré Marta , Malik Vasanti S. , Kendall Cyril W.C. , Manson JoAnn E. , Rimm Eric B. , Willett Walter C. , Sun Qi , Jenkins David J.A. , Hu Frank B. , Sievenpiper John L. 
Published: October 2023

BACKGROUND:
The plant-based Portfolio dietary pattern includes recognized cholesterol-lowering foods (ie, plant protein, nuts, viscous fiber, phytosterols, and plant monounsaturated fats) shown to improve several cardiovascular disease (CVD) risk factors in randomized controlled trials. However, there is limited evidence on the role of long-term adherence to the diet and CVD risk. The primary objective was to examine the relationship between the Portfolio Diet Score (PDS) and the risk of total CVD, coronary heart disease (CHD), and stroke.

METHODS:
We prospectively followed 73 924 women in the Nurses’ Health Study (1984–2016), 92 346 women in the Nurses’ Health Study II (1991–2017), and 43 970 men in the Health Professionals Follow-up Study (1986–2016) without CVD or cancer at baseline. Diet was assessed using validated food frequency questionnaires at baseline and every 4 years using a PDS that positively ranks plant protein (legumes), nuts and seeds, viscous fiber sources, phytosterols (mg/day), and plant monounsaturated fat sources, and negatively ranks foods high in saturated fat and cholesterol.

RESULTS:

During up to 30 years of follow-up, 16 917 incident CVD cases, including 10 666 CHD cases and 6473 strokes, were documented. After multivariable adjustment for lifestyle factors and a modified Alternate Healthy Eating Index (excluding overlapping components), comparing the highest with the lowest quintile, participants with a higher PDS had a lower risk of total CVD (pooled hazard ratio [HR], 0.86 [95% CI, 0.81–0.92];
P
trend
<0.001), CHD (pooled HR, 0.86 [95% CI, 0.80–0.93];
P
trend
=0.0001), and stroke (pooled HR, 0.86 [95% CI, 0.78–0.95];
P
trend
=0.0003). In addition, a 25-percentile higher PDS was associated with a lower risk of total CVD (pooled HR, 0.92 [95% CI, 0.89–0.95]), CHD (pooled HR, 0.92 [95% CI, 0.88–0.95]), and stroke (pooled HR, 0.92 [95% CI, 0.87–0.96]). Results remained consistent across sensitivity and most subgroup analyses, and there was no evidence of departure from linearity for CVD, CHD, or stroke. In a subset of participants, a higher PDS was associated with a more favorable blood lipid and inflammatory profile.

CONCLUSIONS:
The PDS was associated with a lower risk of CVD, including CHD and stroke, and a more favorable blood lipid and inflammatory profile, in 3 large prospective cohorts.
A half-century of global collaboration in science and the “Shrinking World”
Authors: Okamura Keisuke 
Published: October 2023

Abstract
Recent decades have witnessed a dramatic shift in the cross-border collaboration mode of researchers, with countries increasingly cooperating and competing with one another. It is crucial for leaders in academia and policy to understand the full extent of international research collaboration, their country’s position within it, and its evolution over time. However, evidence for such world-scale dynamism is still scarce. This paper provides unique evidence of how international collaboration clusters have formed and evolved over the past 50 years across various scientific publications, using data from OpenAlex, a large-scale open bibliometrics platform launched in 2022. I first examine how the global presence of top-tier countries has changed in 15 natural science disciplines over time, as measured by publication volumes and international collaboration rates. Notably, I observe that the United States and China have been rapidly moving closer together for decades but began moving apart after 2019. I then perform a hierarchical clustering to analyze and visualize the international collaboration clusters for each discipline and period. Finally, I provide quantitative evidence of a “Shrinking World” of research collaboration at a global scale over the past half-century. My results provide valuable insights into the big picture of past, present, and future international collaboration.
Erasmus and the Lady of Loreto
Authors: Fallica Maria 
Published: October 2023

Abstract
One could argue that a mass dedicated to the Virgin of Loreto is an unusual choice for Erasmus. His Liturgy of the Virgin Mother Venerated at Loreto (Virginis Matris apud Lauretum cultae liturgia, 1523, 1525, 1529) would prove such an argument wrong, as this overlooked text reveals much of Erasmus’ theory of accommodatio, his approach to liturgy and the cult of Mary, and his vision of the church and his spiritual exegesis, even in the midst of the Reformation’s turmoil. This article proposes a close reading of the 1525 edition, which will contextualize Erasmus’ portrait of Mary in her moral values and in the de-eroticization and purification of the medieval, Catholic model, which he re-affirms in the same years in which his controversy with Luther unfolded. Moreover, the sermon presents a rarefied and intellectual variant of the feminine imagery of the bride, which I will analyze as being systematically integrated into Erasmus’ theology of progress and ecclesiology.
The Use of Active Coagulation Whole Blood—An Innovative Treatment Strategy for Hard-To-Heal Wounds
Authors: Haim Nadav , Kaufman Jarrod P. , Gurevich Maxim 
Published: October 2023

Background Deep and tunneling wounds are a challenge to apply and maintain most advanced wound dressings to promote effective healing. An autologous whole blood clot is a topical treatment and has been found to be safe and effective in healing cutaneous wounds. The active coagulation whole blood (ACWB) clot treatment, using the patient’s own blood, is used to treat deep and tunneling wounds, by mixing the blood with coagulation components and applying it into the wound cavity allowing the clot to re-form inside the wound. We aimed to explore ACWB treatment in hard-to-heal wounds. Methods 5 patients with multiple comorbidities, exhibiting surgical abdominal wound, chronic pilonidal sinus, stage 4 sacral pressure ulcer with exposed bone, post-amputation surgical site wound, and non-healing wound dehiscence at the site of a prior hip replacement, were all treated with the ACWB clot treatment. Results Complete wound healing was observed in 4/5 cases. In the fifth case, there was a 70% reduction in the depth and surface area of the abdominal surgical wound. Discussion The ACWB treatment was found to be effective in deep wounds with cavities and exposed structures. ACWB, in its flowable form, can effectively provide coverage of the deepest interstices of the wound’s cavities by virtue of its liquid properties, forming a fibrin matrix, mimicking the role of the extracellular matrix. The flowable formulation of ACWB treatment safely and efficiently provides coverage of the entirety of the wound surface to improve the time and process of complex wound surface healing.
Comparison of FDMed and compression molded biocomposites in polylactic acid and food flour waste
Authors: Papa Ilaria , Langella Antonio , Napolitano Francesco , Russo Pietro 
Published: October 2023

Polylactic acid resins are some of the most largely used bioplastics, some characteristics still limit their use, such as in mixing with other polymers or inclusion of additives. Currently, additive manufacturing technologies are enlarging their application sectors because they allow more rational use of raw materials, limiting both production waste and onerous post-processing stages. In this framework, polylactic acid resins, particularly favoured by their slow crystallization rate and the large availability, are some of the most used to obtain commercial filaments for fused deposition modeling. This research is then focused on the processability of the compounds, based on a commercial PLA resin, additivated with 1, 3, and 5 wt% of food flour wastes. The comparison of the performance of the products obtained with conventional and innovative technologies, respectively compression molding and FDM, is the key point of this study. Mechanical and morphological properties, obtained by the two technologies, were evaluated for neat and additivated resins. Preliminary results emphasize that, through compression molded, it was possible to obtain homogeneous components and the natural infilled used acts as reinforcement improving mechanical performances. For the additive technology, the samples obtained show a lower mechanical behavior due to the technological issues of 3d printing.
Bilateral versus single internal mammary artery in diabetic patients: systematic review and meta-analysis
Authors: Parissis Haralabos , Ahmed Suhaib , Al Nasir Jomanah , Khan Jamal , Ferwana Mazen 
Published: October 2023

Objectives Data on bilateral internal mammary artery (BIMA) versus single internal mammary artery (SIMA) on diabetics were analyzed; This is the only meta-analysis, the last 7 years. Methods Medline through PubMed/EMBASE/CINHAL and the Cochrane Central Register of Controlled Trials; 179 articles were studied; 19 studies deemed suitable and were included in the analysis. Results The mortality was 2.41% for BIMA versus 1.71% for SIMA (odds ratio [OR] =  0.95; 95% confidence interval [CI]: 0.74–1.22). Postoperative reopening for bleeding was higher at 3.75% for BIMA versus 2.91% for SIMA (OR =  1.49; 95% CI: 1.15–1.93). The incidence of MI was 0.87% for BIMA versus 0.83% for SIMA (OR =  0.73; 95% CI: 0.37–1.44). Deep sternal wound infection was 3.02% for BIMA and 1.95% for SIMA (OR =  1.57; 95% CI: 1.26–1.95). When skeletonized, the incidence of DSWI was 2.5% for BIMA versus 2.41% for SIMA. There was a significant difference at 5-year survival favoring the BIMA, 85.15% BIMA versus 80.77% SIMA (OR =  1.79; 95% CI: 1.60–2.01). The 10-year overall survival was 74.04% BIMA versus 61.57% SIMA (OR =  1.79; 95% CI: 1.61–1.98). The 15-year survival was 47.08% for BIMA versus 37.06% for SIMA (OR =  1.69; 95% CI: 1.52–1.88). Conclusions Postoperative bleeding was higher in BIMA group. Bilateral internal mammary artery in diabetic patients should be carried out in a skeletonize fashion, to reduce DSWI. There is a survival benefit of using BIMA in diabetics within 5 years of surgery; it remains significant up to 15 years.
Pediatric extracorporeal cardiopulmonary resuscitation for yew cardiotoxicity
Authors: Daniels Zachary , Hays Hannah , Carrillo Sergio , Kamp Anna , Gauntt Jennifer 
Published: October 2023

Introduction English yew is an evergreen conifer frequently planted in household gardens and, when ingested in large doses, results in severe cardiotoxicity characterized by difficult to control ventricular arrhythmias with high rates of mortality. Case report A previously healthy teenage female presented as an out-of-hospital cardiac arrest with refractory ventricular arrhythmias and severe biventricular dysfunction. Due to rapid deterioration in her clinical status, she was cannulated onto venoarterial extracorporeal membrane oxygenation (ECMO) which resulted in rapid normalization of her rhythm and ventricular function. Discussion Our case highlights the importance of keeping a broad differential diagnosis when considering etiologies of ventricular arrhythmias in the pediatric population. The final diagnosis was not made until after discharge and implantable cardiac defibrillator (ICD) placement. Conclusion The delayed diagnosis of this intentional English yew ingestion ultimately resulted subsequent ICD removal. Early ECMO activation in cases of English yew toxicity can be essential for patient survival.
Through the Eyes of Patients: The Effect of Training General Practitioners and Nurses on Perceived Shared Decision-Making Support
Authors: Bos – van den Hoek Danique W. , Smets Ellen M. A. , Ali Rania , Tange Dorien , van Laarhoven Hanneke W. M. , Henselmans Inge 
Published: October 2023

Purpose To examine the effects of training general practitioners and nurses in shared decision-making (SDM) support as perceived by cancer patients and survivors. Design An innovative, experimental design was adopted that included analogue patients (APs), that is, people who have or have had cancer and who imagine themselves in the position of the actor-patient presented in a video. Each AP assessed a video-recorded simulated consultation of a health care professional (HCP) conducted before or after an SDM support training program. The primary outcome was the APs’ perceived SDM support with 13 self-developed items reflecting the perceived patient benefit of SDM support as well as the perceived HCP support behavior. Secondary outcomes included an overall rating of SDM support, AP-reported extent of SDM (CollaboRATE), satisfaction with the communication (Patient Satisfaction Questionnaire), conversation appreciation and helpfulness, as well as decision-making satisfaction and confidence (visual analog scale, 0–100). In addition, patient and HCP characteristics associated with AP-perceived SDM support were examined. Results APs ( n = 131) did not significantly differentiate trained from untrained HCPs in their perceptions of SDM support nor in secondary outcomes. Agreement between APs’ perceptions was poor. The higher the perceived comparability of the consultation with APs’ previous personal experiences, the higher their rating of SDM support. Limitations We used a nonvalidated primary outcome and an innovative study design that should be tested in future work. Conclusions Despite the limitations of the study design, the training seemed to not affect cancer patients’ and survivors’ perceived SDM support. Implications The clinical relevance of the training on SDM support needs to be established. The variation in APs’ assessments suggests patients differ in their perception of SDM support, stressing the importance of patient-tailored SDM support. Highlights Cancer patients and survivors did not significantly differentiate trained from untrained HCPs when evaluating SDM support, and agreement between their perceptions was poor. The clinical relevance of training GPs and nurses in SDM support needs to be established. Patient-tailored SDM support may be recommended, given the variation in APs’ assessments and their possible diverging perceptions of SDM support. This innovative study design (having patients watch and assess videos of simulated consultations made in the context of training evaluation) needs to be further developed.
Biomarkers of oral subacute toxicity of deltamethrin in exposed male Albino rats
Authors: Elbanna Rania , Osman Khaled A , Salama Maher S 
Published: October 2023

Deltamethrin is one of the most effective pyrethroid compounds, widely employed in veterinary medicine, public health, and farming. Deltamethrin-triggered oxidative stress largely causes serious harm to an organism. Acute toxicity of this compound was extensively investigated, while less information is available on its oral sub-acute effects. This study assessed, in the male Albino rats, the effects of oral gavage of either 0.874 mg/kg (0.01 LD50) or 8.740 mg/kg (0.10 LD50) of deltamethrin for successive 14 days to investigate its effects on biomarkers and to detect the tissue injury in rats following subacute deltamethrin treatment. It was found that levels of glutathione peroxidase, superoxide dismutase, and catalase in the brain, kidney, and liver, alkaline phosphatase (ALP), and uric acid in serum, hematocrit, mean corpuscular volume (MCV), white blood cells (WBC)s, eosinophils, and basophils were significantly reduced compared with untreated rats. However, when rats were treated with deltamethrin for successive 14 days, alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) activities in serum and the levels of thiobarbituric acid reactive substances (TBARs) in brain, kidney, and liver, red blood cell distribution width (RDW-CV), total protein, monocytes, and basophils and the ratios of neutrophils to lymphocytes, an aggregated marker of systemic inflammation and systemic immune inflammation indexes, significantly increased compared with the control group. Histologic lesions were observed in the liver, kidney, brain, testis, and epidemies in rats exposed to subacute deltamethrin for 14 days, and most tissues of rats treated with 0.10 LD50 of deltamethrin were more affected than those treated with 0.01 LD50. These findings strongly suggest that subacute exposure to deltamethrin caused significant systemic toxicity through oxidative stress resulting in biochemical and histological changes in the studied tissues. These findings highlight the potential harmful effects of deltamethrin and emphasize the importance of understanding the subacute effects of this compound, particularly in the context of veterinary medicine, public health, and farming.
The Role of Health Insurance Type and Clinic Visit on Hypertension Status Among Multiethnic Chicago Residents
Authors: Luo Jiajun , Krakowka William I. , Craver Andrew , Connellan Elizabeth , King Jaime , Kibriya Muhammad G. , Pinto Jayant , Polonsky Tamar , Kim Karen , Ahsan Habibul , Aschebrook-Kilfoy Briseis 
Published: October 2023

Purpose To investigate the joint relationship of health insurance and clinic visit with hypertension among underserved populations. Design Population-based cohort study. Subjects Data from 1092 participants from the Chicago Multiethnic Prevention and Surveillance Study (COMPASS) between 2013 and 2020 were analyzed. Measures Five health insurance types were included: uninsured, Medicaid, Medicare, private, and other. Clinic visit over past 12 months were retrieved from medical records and categorized into 4 groups: no clinic visit, 1-3 visits, 4-7 visits, >7 visits. Analysis Inverse-probability weighted logistic regression was used to estimate odds ratios (OR) and 95% confidence interval (CI) for hypertension status according to health insurance and clinic visit. Models were adjusted for individual socio-demographic variables and medical history. Results The study population was predominantly Black (>85%) of low socioeconomic status. Health insurance was not associated with more clinic visit. Measured hypertension was more frequently found in private insurance (OR = 6.48, 95% CI: 1.92-21.85) compared to the uninsured group, while 1-3 clinic visits were associated with less prevalence (OR = .59, 95% CI: .35-1.00) compared to no clinic visit. These associations remained unchanged when health insurance and clinic visit were adjusted for each other. Conclusion In this study population, private insurance was associated with higher measured hypertension prevalence compared to no insurance. The associations of health insurance and clinic visit were independent of each other.
Whole Exome Sequencing Identifies Damaging Variants in Indonesians with Clefts
Authors: Aladenika Emmanuel , Maskoen Ani , Awotoye Waheed , Abdulaziz Rasyid , Alade Azeez , Nasroen Saskia Lenggogeni , Oladayo Abimbola , Busch Tamara , Sarilita Erli , Butali Azeez 
Published: October 2023

Objectives The interaction between genomics, genetic and environmental factors have been implicated in non-syndromic orofacial cleft development. In the current study, we investigated the contributions of rare and novel genetic variants in known cleft genes using whole exome sequencing (WES) data of Indonesians with non-syndromic orofacial clefts. Design WES was conducted on 6 individuals. Variants in their exons were called and annotated. These variants were filtered for novelty and rarity using MAF of 0 and 1%. Setting Hospital in Indonesia. Patients/Participants Indonesians with non-syndromic orofacial clefts. Interventions Deleterious variants were prioritized. Pathogenic amino acid changes effect on protein structure and function were identified using HOPE. Main Outcome Measure(s) Rare and novel variants in known cleft genes were filtered and their deleteriousness were predicted using polyphen, SIFT and CADD. Results We identified rare (MAF <1%) deleterious variants in 4 craniofacial genes namely MMACHC (rs371937044, MAF = 0.00011). SOS1 (rs190222208, MAF = 0.00045), TULP4 (rs199583035, MAF = 0.067), and MTHFD1L (rs143492706, MAF = 0.0044). MMACHC has a mouse knockout model with facial cleft and failure of palatal fusion. The individual with variant in MMACHC presented with nsCPO. Conclusions Our study provides additional evidence for the role of TULP4, SOS1, MTHFD1L and MMACHC genes in nsOFC development. This is the first time MMACHC is implicated in nsOFC development in humans.