bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nociception and baroreceptor stimulation in hypertension‐prone men and women
Authors: Al'Absi Mustafa , France Christopher R. , Ring Christopher , France Janis , Harju Angie , Mcintyre David , Wittmers Lorentz E. 
Published: February 2005

AbstractWe examined the effects of baroreceptor stimulation on nociceptive responding in men and women with a positive or negative parental history of hypertension. The effects of three baroreceptor conditions (stimulation, inhibition, and control) on subjective pain and nociceptive responding were evaluated during electrocutaneous sural nerve stimulation. Pain ratings were lower in men with positive parental history relative to men with negative parental history, but this difference was not found in women. Both stimulatory and inhibitory baroreceptor conditions were associated with reduced pain reports compared to the control condition. There were no significant differences in nociceptive responding as a function of parental history of hypertension. Although this study confirms a link between hypoalgesia and risk for hypertension in men, it does not support the hypothesis that this attenuated pain perception is due to enhanced baroreceptor activity.
Strategic control and medial frontal negativity: Beyond errors and response conflict
Authors: Bartholow Bruce D. , Pearson Melanie A. , Dickter Cheryl L. , Sher Kenneth J. , Fabiani Monica , Gratton Gabriele 
Published: February 2005

AbstractErrors in timed choice tasks typically produce an error‐related negativity (ERN) in the event‐related potential (ERP). The error specificity of the ERN has been challenged by studies showing a correct response negativity (CRN). Forty‐five participants engaged in a flanker task in which both compatibility between flankers and target and the probability of compatible flankers were manipulated. Correct responses elicited a CRN, the amplitude of which increased with the degree of mismatch between the presence of conflict and conflict probability, even on low‐conflict (compatible) trials. The fronto‐central N2 component was larger on high‐conflict (incompatible) correct response trials. However, in contrast to some recent accounts, this N2 was largest for highly probable stimuli. These findings suggest revision to models of the effects of conflict on response‐related negativity to account for strategic adjustments made in preparation for the response.
The relationship between women's subjective and physiological sexual arousal
Authors: Rellini Alessandra H. , McCall Katie M. , Randall Patrick K. , Meston Cindy M. 
Published: February 2005

AbstractPrevious literature presents discordant results on the relationship between physiological and subjective sexual arousal in women. In this study, the use of hierarchical linear modeling (HLM) revealed a significant concordance between continuous measures of physiological and subjective sexual arousal as assessed during exposure to erotic stimuli in a laboratory setting. We propose that past studies that have found little or no association between the two measures may have been in part limited by the methodology and statistical analyses employed.
Event‐related potential correlates of task switching and switch costs
Authors: Kieffaber Paul D. , Hetrick William P. 
Published: February 2005

AbstractStudies of task switching demonstrate that task switches are associated with response costs and that these costs are reduced when a cue is presented in advance of a switch. The present study examined cortical event‐related potential correlates of task switching and switch costs in 39 participants during a cued match/mismatch discrimination task. Compared with non‐switch trials, switch trials were associated with a larger cue‐related, anticipatory P3b‐like waveform. Switch trials were also associated with smaller target‐related, stimulus‐dependent P2 and P3‐like components. Moreover, the switch‐related amplitude variability in the P3b to the cue and the P2 to the target were associated with unique components of the residual switch costs. The results support an integrated model of task switching with complementary yet distinct roles for anticipatory and stimulus‐dependent processes in task switching and switch costs.
Phyto‐specific 16S rDNA PCR primers for recovering algal and plant sequences from mixed samples
Authors: STILLER JOHN W. , McCLANAHAN ANA 
Published: February 2005

AbstractMolecular environmental sampling of the phototrophic complexity in a given environment may be important in a number of research disciplines. Because of the broad evolutionary diversity of photosynthetic organisms, however, primers that can recover sequences from all phototrophs also target other organisms, often preferentially. Therefore, PCR primers that selectively amplify genes of phototrophs over those of other prokaryotic and eukaryotic organisms could prove extremely useful. Here we report two such primers that target 16S rDNA from Cyanobacteria and eukaryotic plastids, but do not amplify genes from abundant Bacteria in a mixed sample.
Polymorphic microsatellite loci in a plural breeder, the grey‐capped social weaver (Pseudonigrita arnaudi), isolated with an improved enrichment protocol using fragment size‐selection
Authors: MCRAE SUSAN B. , EMLEN STEPHEN T. , RUBENSTEIN DUSTIN R. , BOGDANOWICZ STEVEN M. 
Published: February 2005

AbstractWe isolated polymorphic microsatellite loci (mean number of alleles = 12) from the grey‐capped social weaver (Pseudonigrita arnaudi) genome. We designed an experiment to test whether the addition of a fragment size‐selection step at either of two different stages in an enrichment protocol increased the average insert size in transformants. Fragment size‐selection produced significantly larger average insert sizes among our positive (microsatellite‐containing) transformants. Size‐selection just before ligation to the cloning vector was most effective. Eighteen independently‐assorting polymorphic loci were characterized. Some amplified in other ploceid weavers and related species. Collectively, the markers provide a mean exclusion probability of 0.9999 for parentage analysis, and will be a powerful tool for reconstructing extended family membership in this highly social bird.
Trinucleotide microsatellite loci in the yellow dung fly Scathophaga stercoraria (Diptera: Scathophagidae)
Authors: WATTS PHILLIP C. , DALY DEREK , KAYANI ANNA , CULVER FRANCESCA A. , KELLY VICTORIA M. , PARKER GEOFF A. , KEMP STEPHEN J. 
Published: February 2005

AbstractWe describe six polymorphic, trinucleotide microsatellite loci that were isolated from the yellow dung fly Scathophaga stercoraria (Diptera: Scathophagidae), a model system for examining the mechanisms behind sperm competition in species with internal fertilization. These microsatellites yielded between 3 and 11 alleles per locus in a sample of 20–48 dung flies collected from several sites in Cheshire, UK. Observed and expected heterozygosities varied between 0.195 and 0.900 and 0.335–0.842, respectively. These markers should allow us to investigate postcopulatory sexual selection processes in this fly much more fully than has been possible in the past.
Novel microsatellite loci identified from the Australian eastern small‐eyed snake (Elapidae: Rhinocephalus nigrescens) and cross species amplification in the related genus Suta
Authors: STAPLEY JESSICA , HAYES CHRISTINE M. , WEBB JONATHAN K. , KEOGH J. SCOTT 
Published: February 2005

AbstractA total of 15 microsatellite primers pairs were developed for the Australian small‐eyed snake Rhinoplocephalus nigrescens. Five primers were used to screen 93 individuals of R. nigrescens and were also tested against eight species of the closely related genus Suta. Allelic diversity in R. nigrescens was high in three loci (12–27) and there was high heterozygosity (0.58–0.82). Observed heterozygosity did not deviate from Hardy–Weinberg expectations for the five loci tested. These primers will be useful in studies of population genetics and mating systems of small‐eyed snakes and related species.
Characterization of 14 polymorphic microsatellite markers for the black‐tailed prairie dog (Cynomys ludovicianus)
Authors: JONES RYAN T. , MARTIN ANDREW P. , MITCHELL ADAM J. , COLLINGE SHARON K. , RAY CHRIS 
Published: February 2005

AbstractWe report the development of 14 polymorphic microsatellite markers for the black‐tailed prairie dog, Cynomys ludovicianus. The number of alleles per locus ranges from three to 11, and the observed heterozygosity ranges from 0.37500 to 1.0000. These markers will benefit studies of landscape effects on prairie dog migration, disease dynamics, and conservation efforts.
Microsatellite DNA markers for population genetic studies and parentage assignment in cobia, Rachycentron canadum
Authors: PRUETT CHRISTIN L. , SAILLANT ERIC , RENSHAW MARK A. , PATTON JOHN C. , REXROAD CAIRD E. , GOLD JOHN R. 
Published: February 2005

AbstractTwenty nuclear‐encoded microsatellites from a genomic DNA library of cobia, Rachycentron canadum, were isolated and characterized. The microsatellites include two tetranucleotide, one trinucleotide, three combination tetranucleotide/dinucleotide, nine dinucleotide, and five imperfect (dinucleotide) repeat motifs. Gene diversity ranged between zero to 0.910; the number of alleles among a sample of 24 fish ranged from one to 15. Cobia support an important recreational fishery in the southeastern United States and recently have become of interest to aquaculture. The microsatellites developed will be useful tools for studying both population genetics (e.g. stock structure, effective population size) and inheritance of traits important to aquaculture.
Isolation and characterization of polymorphic microsatellite markers in the gray, short‐tailed opossum (Monodelphis domestica)
Authors: GOUIN NICOLAS , WESTENBERGER SCOTT J. , MAHANEY SUSAN M. , SAMOLLOW PAUL B. 
Published: February 2005

AbstractTwenty‐six polymorphic microsatellite markers were isolated from (AC)n and (AG)n microsatellite‐enhanced genomic libraries of the gray, short‐tailed opossum Monodelphis domestica. All 26 loci showed high allelic diversity, with allele numbers ranging from five to 11 in a subset of 35 animals. Normal Mendelian inheritance was confirmed for 24 loci by analysing allelic segregation in 10, two‐generation, families. Non‐amplifying (null) alleles were detected at two loci, which we recommend be used only if pedigree data are available. We conclude that all of these microsatellite markers would be useful for quantitative trait locus mapping and population genetic studies.
Development and characterization of microsatellite DNA primers for ferruginous pygmy‐owls (Glaucidium brasilianum)
Authors: PROUDFOOT G. , HONEYCUTT R. , DOUGLAS SLACK R. 
Published: February 2005

AbstractTen polymorphic microsatellite loci were isolated and characterized for the ferruginous pygmy owl (Glaucidium brasilianum). Observed and expected heterozygosity were 0.07–0.85 and 0.1–0.88, respectively. Allele numbers ranged from five to 16 per locus.
pathmatrix: a geographical information system tool to compute effective distances among samples
Authors: RAY NICOLAS 
Published: February 2005

Abstract pathmatrix is a tool used to compute matrices of effective geographical distances among samples using a least‐cost path algorithm. This program is dedicated to the study of the role of the environment on the spatial genetic structure of populations. Punctual locations (e.g. individuals) or zones encompassing sample data points (e.g. demes) are used in conjunction with a species‐specific friction map representing the cost of movement through the landscape. Matrices of effective distances can then be exported to population genetic software to test, for example, for isolation by distance. pathmatrix is an extension to the geographical information system (GIS) software arcview 3.x.
Microsatellite loci in the stable fly, Stomoxys niger niger (Diptera: Muscidae) on La Réunion Island
Authors: GILLES J. , LITRICO I. , DUVALLET G. 
Published: February 2005

AbstractSix polymorphic microsatellite markers have been isolated from a microsatellite‐enriched DNA library from the stable fly, Stomoxys niger niger. These loci exhibited five to 10 alleles per locus and an expected heterozygosity ranging from 0.57 to 0.81 in the studied populations from La Réunion Island (Indian Ocean). They should therefore be useful for the study of genetic diversity and population structure of S. niger. In addition, cross‐species amplification of four of these six loci in the closely allied species Stomoxys calcitrans produced interpretable results, two of which would be useful for population biology studies.
hp‐rare 1.0: a computer program for performing rarefaction on measures of allelic richness
Authors: KALINOWSKI STEVEN T. 
Published: February 2005

AbstractThe number of alleles in a sample (allelic richness) is a fundamental measure of genetic diversity. However, this diversity measure has been difficult to use because large samples are expected to contain more alleles than small samples. The statistical technique of rarefaction compensates for this sampling disparity. Here I introduce a computer program that performs rarefaction on private alleles and hierarchical sampling designs.
Microsatellite loci for Myrmica kotokui and their application in some congeneric ant species distributed in northern Japan
Authors: AZUMA N. , TAKAHASHI J. , KIKUCHI T. , YOSHIMURA M. , ONOYAMA K. , HIGASHI S. 
Published: February 2005

AbstractWe developed seven polymorphic microsatellite loci for Myrmica kotokui from RAPD (rapid analysis of polymorphic DNA) fragments. These loci showed two to six alleles with expected and observed heterozygosity of 0.13–0.73 and 0.14–0.78, respectively. These loci are transferable to the congeneric species as Myrmica rubra, Myrmica luteola and Myrmica taediosa from northern Japan. These loci will allow analyses of genetic structure of Myrmica species at both the colony level and population levels.
Isolation of polymorphic tetranucleotide microsatellite markers for the grey‐headed robin (Poecilodryas albispecularis)
Authors: BARDELEBEN CAROLYNE , DELANEY KATHLEEN SEMPLE , AUSTIN JEREMY , MOORE RACHAEL L. 
Published: February 2005

AbstractWe isolated 12 polymorphic microsatellite markers for the grey‐headed robin Poecilodryas albispecularis from genomic libraries enriched for (AAGG)n and (AACC)n repetitive elements and characterized them in 12 individuals. The number of alleles ranges from three to nine per locus with the observed heterozygosity ranging from 0.33 to 0.90. These markers will be useful for analysis of questions concerning population genetic structure and testing models of speciation.
Isolation and characterization of new microsatellite markers in mango (Mangifera indica)
Authors: HONSHO CHITOSE , NISHIYAMA KIYOMI , EIADTHONG WICHAN , YONEMORI KEIZO 
Published: February 2005

AbstractSix microsatellite loci that were isolated from a microsatellite‐enriched genomic library of mango (Mangifera indica) along with their specific primer sets were each characterized by using 36 cultivars collected mainly in Thailand. The observed and expected heterozygosities ranged from 0 to 0.83 and from 0.29 to 0.73, respectively. The number of putative alleles are two to six. Three of the six alleles have frequencies of over 75%. The high frequency may be attributed to the bias in the origin of cultivars. Among 36 mango cultivars tested, 29 cultivars showed a unique pattern by six primer sets, whereas seven cultivars cannot be identified because of genotype similarities. This suggests the potentials for identification of mango cultivars by microsatellite markers.
A microsatellite‐based method for genotyping diploid and triploid water frogs of the Rana esculenta hybrid complex
Authors: CHRISTIANSEN DITTE G. 
Published: February 2005

Abstract Rana esculenta is a hybrid between Rana lessonae (LL) and Rana ridibunda (RR), and hybrids may be diploid (LR) or triploid (LLR or LRR). Genotypes can be roughly determined from erythrocyte size and morphometry in adult frogs, but accurate genotyping requires more labourious methods. Here I demonstrate that both the L and R genomes have specific microsatellite alleles, and that genotype and ploidy can be accurately inferred from the quantitative ratio of PCR‐amplified (polymerase chain reaction‐amplified) genome‐specific alleles. This method greatly facilitates genotyping in DNA studies of the R. esculenta complex and allows analysis of badly preserved samples and embryos.
Development of microsatellites DNA markers in the cultivated seaweed, Gracilaria chilensis (Gracilariales, Rhodophyta)
Authors: GUILLEMIN M.‐L. , DESTOMBE C. , FAUGERON S. , CORREA J. A. , VALERO M. 
Published: February 2005

AbstractThe red algae Gracilaria chilensis is extensively cultivated for agar production. In spite of its commercial significance as the first algal resource in Chile, no information is available on the pattern of genetic diversity. In this paper, we isolated six polymorphic microsatellite markers from a G. chilensis‐enriched DNA library. Genetic diversity was assessed in two natural populations revealing relatively low levels of heterozygosity ranging from 0.00 to 0.51. The six loci developed here are good candidates to assess the level of genetic resources within this species, which probably suffered from over‐exploitation in several localities along the Chilean coast.
Repair of Radial Fractures in Toy Breed Dogs with Self‐Reinforced Biodegradable Bone Plates, Metal Screws, and Light‐Weight External Coaptation
Authors: Saikku‐Bäckström Anu , Räihä Jan E. , Välimaa Tero , Tulamo Riitta‐Mari 
Published: February 2005

Objective— To describe a surgical technique for, and outcome after, treatment of radial fractures with biodegradable self‐reinforced polylactide plates and metal screws, and external coaptation.Study Design— Prospective clinical study.Sample Population— Eleven Toy breed dogs.Methods— Radial fractures were repaired by application of a single or 2 stacked biodegradable self‐reinforced polylactide plates (poly‐l/d, l‐lactide, stereocopolymer [ll‐and dl‐lactide ratio 70/30]; SR‐PLA (70/30) implants) secured with metal screws, and light‐weight external coaptation. Healing was evaluated clinically and by radiography at 2, 4, 6, 8, 9, 12, 24–26 weeks, and at 1 and 2 years. Owners were interviewed 3 years after surgery.Results— Radial fracture lines disappeared within 4–14 weeks in 10 dogs; an implant failed in 1 dog. Ambulation was excellent for healed fractures. Excessive skin tension led to removal of implants in 1 dog and suture repair in another dog. No foreign body reaction from implant degradation was observed and the plate was usually no longer palpable at 2 years. One dog had a fracture through a screw hole at 1 year.Conclusion— Healing and complication rates after repair of radial fractures with SR‐PLA (70/30) plates were considered similar or better than reported after repair with metallic plates or external fixation in Toy breed dogs. No radiographic signs of osteopenia were identified under the plate during follow‐up.Clinical Relevance— Biodegradable polylactide plates could be considered as an alternative to metal plates for radial fracture repair in Toy breed dogs, however available plates are likely not strong enough when used as a single plate. Implant removal is usually not needed.
Coxofemoral Arthroscopy and Total Hip Arthroplasty for Management of Intermediate Grade Fibrosarcoma in a Dog
Authors: Scherrer William , Holsworth Ian , Goossens Marielle , Schulz Kurt 
Published: February 2005

Objective— To report the use of arthroscopy and total hip arthroplasty (THA) for management of intermediate grade fibrosarcoma of the femoral head in a dog.Study Design— Clinical case report.Animal— A 6‐year‐old spayed female Rottweiler presented for evaluation of acute‐onset of lameness of the right pelvic limb of approximately 10 days duration.Methods— Coxofemoral arthroscopy was performed to obtain fine needle aspiration and Jamshidi biopsy of a lytic lesion of the femoral head. A cemented THA was performed removing as much of the femoral neck and proximal femur as possible while not compromising implant or joint stability.Results— Histologic evaluation of specimens from the femoral head and neck remnants confirmed an intermediate grade fibrosarcoma. Fifteen months after THA, subjectively there was normal use of the right limb with only limited decrease in range of motion and mild muscular atrophy. Radiographs demonstrated stationary positioning of the THA implants.Conclusions— Arthroscopy of the coxofemoral joint is an effective diagnostic tool for evaluation of disease of the articular structures and canine total hip replacement may be an appropriate means of limb salvage in rare cases of disease of the femoral head.Clinical Relevance— This case provides support for the use of coxofemoral arthroscopy as a diagnostic tool as well as an aid in acquiring representative intraarticular tissue samples. Additionally, the case presented demonstrates the potential use of THA as a limb sparing technique in cases of disease affecting the femoral head of dogs.
Arthroscopic Treatment of Temporomandibular Joint Sepsis in a Horse
Authors: Carmalt James L. , Wilson David G. 
Published: February 2005

Objective— To report arthroscopic debridement and lavage of a septic temporomandibular joint (TMJ) in a horse.Design— Clinical case report.Animals— A 12‐year‐old Thoroughbred mare.Methods— Arthroscopic investigation of the dorsal joint pouch of the right TMJ was made through a stab incision into the dorsocaudal compartment. Mechanized resection of synovium and fibrinous debris combined with copious lavage, and intra‐articular and systemic antibiotic administration was used to treat the septic TMJ.Results— Arthroscopic debridement and lavage of the TMJ, in combination with intra‐articular and systemic antimicrobials resulted in resolution of sepsis. Eight months post‐operatively, there was no clinical evidence of degenerative joint disease or ankylosis of the TMJ.Conclusion— TMJ sepsis is rare in horses, however, standard arthroscopic equipment can be used in the management of this condition with minimal complications.Clinical Relevance— Arthroscopic debridement and lavage should be considered for evaluation and initial treatment of TMJ sepsis in horses.
Clinical Evaluation of Random Skin Flaps Based on the Subdermal Plexus Secured with Sutures or Sutures and Cyanoacrylate Adhesive for Reconstructive Surgery in Dogs
Authors: De Carvalho Vasconcellos Carmen Helena , Matera Julia Maria , Zaidan Dagli Maria Lúcia 
Published: February 2005

Objective— To evaluate the use of subdermal plexus skin flaps for closing defects after excision of cutaneous and subcutaneous tumors in dogs and to compare outcome of flaps secured with sutures and those secured with butyl‐cyanoacrylate and intermittent sutures.Study Design— Clinical study.Animals— Fifteen dogs.Methods— After excision of cutaneous or subcutaneous tumors the skin defect was reconstructed by random flaps based on the subdermal plexus. Flap skin edges were apposed with simple interrupted 4‐0 monofilament nylon sutures (group 1; 5 dogs) or nylon sutures alternated with butyl‐cyanoacrylate adhesive (group 2; 10 dogs). Flaps were evaluated every 48 hours when bandages were changed, until complete healing.Results— Random flaps based on the subdermal plexus were effectively used to close wound defects; mean flap survival was 89%. Partial flap necrosis occurred in 4 dogs. Wound margins apposed with butyl‐cyanoacrylate had thinner and more esthetic scars than sutured margins.Conclusion— Random flaps based on the subdermal plexus proved to be versatile for covering limb wounds after excision of cutaneous or subcutaneous tumors. Mean survival rate was comparable to that reported for axial pattern flaps. Butyl‐cyanoacrylate adhesive was easy to apply, allowed accurate margin apposition with good cosmetic outcome and reduced sutures needed.Clinical Relevance— Cyanoacrylate adhesive should be considered in lieu of suture closure to secure random skin flaps based on the subdermal plexus in dogs.