bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outward-oriented sites within clustered CTCF boundaries are key for intra-TAD chromatin interactions and gene regulation
Authors: Ge Xiao , Huang Haiyan , Han Keqi , Xu Wangjie , Wang Zhaoxia , Wu Qiang 
Published: December 2023

AbstractCTCF plays an important role in 3D genome organization by adjusting the strength of chromatin insulation at TAD boundaries, where clustered CBS (CTCF-binding site) elements are often arranged in a tandem array with a complex divergent or convergent orientation. Here, using Pcdh and HOXD loci as a paradigm, we look into the clustered CTCF TAD boundaries and find that, counterintuitively, outward-oriented CBS elements are crucial for inward enhancer-promoter interactions as well as for gene regulation. Specifically, by combinatorial deletions of a series of putative enhancer elements in mice in vivo or CBS elements in cultured cells in vitro, in conjunction with chromosome conformation capture and RNA-seq analyses, we show that deletions of outward-oriented CBS elements weaken the strength of long-distance intra-TAD promoter-enhancer interactions and enhancer activation of target genes. Our data highlight the crucial role of outward-oriented CBS elements within the clustered CTCF TAD boundaries in developmental gene regulation and have interesting implications on the organization principles of clustered CTCF sites within TAD boundaries.
A dodecamethoxy[6]cycloparaphenylene consisting entirely of hydroquinone ethers: unveiling in-plane aromaticity through a rotaxane structure
Authors: Narita Naoki , Kurita Yusuke , Osakada Kohtaro , Ide Tomohito , Kawai Hidetoshi , Tsuchido Yoshitaka 
Published: December 2023

Abstract[n]Cycloparaphenylenes ([n]CPPs, where n is the number of phenylene groups), consisting of 1,4-linked phenylene unit, have attracted much attention due to their cyclic π-conjugated structures and physical properties. However, functionalizing of the benzene rings of smaller [n]CPPs (n < 7) has been a challenge due to ring strain and steric hindrance of the substituents that hampers their synthesis. Here we show successful synthesis of a new [6]CPP derivative with twelve methoxy groups at the 2,5-positions of all benzene rings by utilizing our developed CPP synthesis method via a macrocyclic gold complex. This molecule exhibited a significantly higher oxidation potential caused by the electron-donating ability of the methoxy groups and the tubular molecular conformation, allowing facile oxidation to give dicationic species with in-plane aromaticity. Furthermore, this molecule successfully included with the guest molecules with a flexible alkyl chain in the cavity, enabling the creation of a CPP-based rotaxane, which exploited its mechanically interlocked molecular structure to the first experimental observation that the in-plane aromaticity in the center of the macrocycle.
Photon-phonon collaboratively pumped laser
Authors: Fu Yu , Liang Fei , He Cheng , Yu Haohai , Zhang Huaijin , Chen Yan-Feng 
Published: December 2023

AbstractIn 1917, Einstein considered stimulated photon emission of electron radiation, offering the theoretical foundation for laser, technically achieved in 1960. However, thermal phonons along with heat creation of non-radiative transition, are ineffective, even playing a detrimental role in lasing efficiency. Here, we realize a photon-phonon collaboratively pumped laser enhanced by heat in a counterintuitive way. We observe a laser transition from phonon-free 1064 nm lasing to phonon-pumped 1176 nm lasing in Nd:YVO4 crystal, associated with the phonon-pumped population inversion under high temperatures. Moreover, an additional temperature threshold (Tth) appears besides the photon-pump power threshold (Pth), and a two-dimensional lasing phase diagram is verified with a general relation ruled by Pth = C/Tth (constant C upon loss for a given crystal), similar to Curie’s Law. Our strategy will promote the study of laser physics via dimension extension, searching for highly efficient and low-threshold laser devices via this temperature degree of freedom.
Functional and genomic characterization of a novel probiotic Lactobacillus johnsonii KD1 against shrimp WSSV infection
Authors: Dekham Kanokwan , Jones Samuel Merryn , Jitrakorn Sarocha , Charoonnart Patai , Thadtapong Nalumon , Intuy Rattanaporn , Dubbs Padungsri , Siripattanapipong Suradej , Saksmerprome Vanvimon , Chaturongakul Soraya 
Published: December 2023

AbstractWhite Spot syndrome virus (WSSV) causes rapid shrimp mortality and production loss worldwide. This study demonstrates potential use of Lactobacillus johnsonii KD1 as an anti-WSSV agent for post larva shrimp cultivation and explores some potential mechanisms behind the anti-WSSV properties. Treatment of Penaeus vannamei shrimps with L. johnsonii KD1 prior to oral challenge with WSSV-infected tissues showed a significantly reduced mortality. In addition, WSSV copy numbers were not detected and shrimp immune genes were upregulated. Genomic analysis of L. johnsonii KD1 based on Illumina and Nanopore platforms revealed a 1.87 Mb chromosome and one 15.4 Kb plasmid. Only one antimicrobial resistance gene (ermB) in the chromosome was identified. Phylogenetic analysis comparing L. johnsonii KD1 to other L. johnsonii isolates revealed that L. johnsonii KD1 is closely related to L. johnsonii GHZ10a isolated from wild pigs. Interestingly, L. johnsonii KD1 contains isolate-specific genes such as genes involved in a type I restriction-modification system and CAZymes belonging to the GT8 family. Furthermore, genes coding for probiotic survival and potential antimicrobial/anti-viral metabolites such as a homolog of the bacteriocin helveticin-J were found. Protein–protein docking modelling suggests the helveticin-J homolog may be able to block VP28–PmRab7 interactions and interrupt WSSV infection.
Abundant chaos in a mixer model with a hysteretic iron core inductance
Authors: Aminou M. , Simo Domguia U. , Oumarou S. A. , Woafo P. 
Published: December 2023

AbstractIndustrial mixers are equipment used in food, drug, chemical and semiconductor industries. Chaotic mixing has been proposed to improve the degree of homogeneity and reduce the energy consumption. This paper deals with dynamical studies of a mixer model with complex rotational movements. The complexity is generated by an inductance with hysteretic characteristics. Mathematical methods and numerical simulations are used to display the different dynamical states which are period-nT, pulse, bursting and chaotic signals. Good agreement is found between the mathematical and numerical results. In general, it is found that chaos is highly abundant in the model.
Suppression of weed and insect populations by living and straw mulches in sesame (Sesamum indicum L.)
Authors: Azimi Solmaz , Amini Rouhollah , Hosseingolizadeh Majid 
Published: December 2023

AbstractIn order to evaluate the effect of different weed management treatments on weeds, pest and natural enemies populations in sesame (Sesamum indicum L.), a 2-year study was conducted in East Azarbaijan, Iran in 2020–2021. The study was conducted based on randomized complete block design with four replications. The weed management treatments consisted of trifluralin use (960 g ai ha−1), wheat straw mulch (WSM), living mulches of fenugreek (Trigonella foenum-graecum L.) (FLM), bitter vetch (Vicia ervilia L.) (VLM), calendula (Calendula officinalis L.) (CLM) and one-time hand weeding (OHW). The effect of weed management treatment was significant on densities of insect pests, natural enemies and weed and also weed biomass and sesame seed yield. The lowest densities of insect pests including Myzus persicae, Brevicoryne brassicae, Helicoverpa armigera and Spodoptera exigua were observed in CLM treatment. Also, the highest densities of natural enemies Coccinella septompunctata, Coccinella undecimpunctata and Orius niger were observed in CLM treatment. The highest reductions in grass (51.0%), broadleaf (72.0%), and total (62.6%) weed biomasses and highest seed yield (1456 kg ha−1) were obtained in OHW. The seed yields in CLM and WSM treatments were not significantly different with trifluralin treatment and could be recommended in sustainable production of sesame.
Machine learning algorithms for a novel cuproptosis-related gene signature of diagnostic and immune infiltration in endometriosis
Authors: Wang Jiajia , Lu Yiming , Sun Guangyu , Fang Zhihao , Xing Zhiyong , Nong Weihua , Wei Yunbao , Wang Shan , Shi Guiling , Dong Mingyou , Wang Junli 
Published: December 2023

AbstractEndometriosis (EMT) is an aggressive disease of the reproductive system, also called “benign cancer”. However, effective treatments for EMT are still lacking in clinical practice. Interestingly, immune infiltration is significantly involved in EMT pathogenesis. Currently, no studies have shown the involvement of cuproptosis-related genes (CRGs) in regulating immune infiltration in EMT. This study identified three CRGs such as GLS, NFE2L2, and PDHA1, associated with EMT using machine learning algorithms. These three CRGs were upregulated in the endometrium of patients with moderate/severe EMT and downregulated in patients with infertility. Single sample genomic enrichment analysis (ssGSEA) revealed that these CRGs were closely correlated with autoimmune diseases such as systemic lupus erythematosus. Furthermore, these CRGs were correlated with immune cells such as eosinophils, natural killer cells, and macrophages. Therefore, profiling patients based on these genes aid in a more accurate diagnosis of EMT progression. The mRNA and protein expression levels of GLS, NFE2L2 and PDHA1 were validated by qRT-PCR and WB studies in EMT samples. These findings provide a new idea for the pathology and treatment of endometriosis, suggesting that CRGs such as GLS, NFE2L2 and PDHA1 may play a key role in the occurrence and development of endometriosis.
Study on wind load characteristics of stadium under tornado
Authors: Yuan Zhongya , Zhang Zhibo , Tang Hao 
Published: December 2023

AbstractThe stadium is a wind-sensitive structure, and the tornado has a greater damage to the stadium. The Ward tornado generating device was used to simulate the wind load characteristics of the stadium during tornado action in order to investigate the wind load characteristics of the stadium. The results of this paper are compared with the experimental results to verify the accuracy of the simulation method. The wind load characteristics of the stadium and its most vulnerable location during a tornado are ascertained by examining the wind pressure coefficient and wind speed distribution law of the stadium’s upper and lower surfaces with different swirl ratio. The influence of uniform wind and wind profile on the wind load of the stadium is explored by comparing and analyzing the wind pressure coefficient of the stadium surface with and without wind profile at the entrance of the wind field. The impact of varying ground roughness on the stadium is compared in order to ascertain how it affects the wind speed, stadium wind pressure, and vortex core radius. The vorticity of the stadium in the tornado wind field with different swirl ratios was compared and analyzed, and the influence of the stadium on the vorticity development of the wind field was explored. This study reveals the effects of swirl ratio, ground roughness and wind profile on the wind pressure, wind speed and vorticity of the stadium under tornadoes, and determines the most vulnerable location and the most unfavorable factors. The above research is helpful to provide reference for disaster prevention and mitigation of stadium structure design.
K-means-based heterogeneous tunneling data analysis method for evaluating rock mass parameters along a TBM tunnel
Authors: Wang Ruirui , Zhang Lingli 
Published: December 2023

AbstractRapid and accurate judgment of the rock mass condition is the key to guaranteeing the safety and efficiency of tunnel boring machine (TBM) tunneling. This paper proposes a method for evaluating rock mass parameters based on K-means clustering, grouping tunneling areas according to the values of TBM tunneling parameters. A dataset including rock mass and TBM tunneling data is treated by logistic normalization and principal component analysis (PCA), and large volumes of tunneling data with different features are transformed into appropriate volumes of dimensionless data. K-means clustering is used, samples are grouped according to the values of tunneling data, and the specific ranges as defined by clustering are regarded as the unified evaluated results of each group. Based on the C1 part of the Pearl Delta water resources allocation project, 100 training samples and 30 testing samples were field-collected, and the proposed method was realized by the training samples and verified by the testing samples. The evaluation accuracies of uniaxial compressive strength (UCS), and joint frequency (Jf) were 90%, and 86.7% respectively, demonstrating that the evaluation had acceptable values, and the proposed method was greatly helpful for judging rock conditions.
Primäre und sekundäre Lebertumore – aus Sicht der Nuklearmedizin
Authors: Holzgreve Adrien , Ilhan Harun , Unterrainer Lena M. , Kircher Malte , Lapa Constantin , Enke Johanna S. 
Published: December 2023

ZusammenfassungKonventionelle, radiologische Modalitäten wie die Ultraschalldiagnostik, CT- und
MRT-Bildgebung sind der klinische Standard in der onkologischen Bildgebung primärer und
sekundärer Lebertumore. In den letzten Jahrzehnten konnten nuklearmedizinische Verfahren,
darunter insbesondere die PET/CT-Bildgebung, zusätzliche, molekulare Informationen liefern,
die maßgeblich zur weiteren Optimierung der Stadieneinteilung und Risikostratifizierung
beigetragen haben. Neben FDG als „Standard“-Radiopharmakon der PET/CT-Bildgebung werden in
diesem Artikel weitere, spezifischere Radiopharmaka und neue Entwicklungen beschrieben.
Editorial
Authors: Proff Peter 
Published: December 2023

Sehr verehrte Frau Kollegin, sehr geehrter Herr Kollege,der Blick über den Tellerrand zeigt eindrucksvoll, dass die
Kieferorthopädie unverzichtbarer und integraler Bestandteil eines
synoptischen zahnärztlichen Versorgungskonzeptes ist, aber auch welche
ausgezeichneten Möglichkeiten sich durch die Zusammenarbeit mit den
Fachdisziplinen Parodontologie, Zahnerhaltung, Prothetik und Chirurgie
eröffnen. In den letzten zehn Jahren wurden in der gesamten Zahnheilkunde
nicht nur neue Lösungsstrategien für das Behandlungsmanagement
komplexer Fälle entwickelt, sondern auch die allgemeine
Versorgungsqualität in der Fläche auf ein hohes Niveau gehoben.
Retainer im MRT – Belassen oder Entfernen?
Authors: Roser C. J. , Jürchott A. , Bendszus M. , Heiland S. , Lux C. J. , Hilgenfeld T. 
Published: December 2023

ZusammenfassungDie Magnetresonanztomographie stellt einen wesentlichen Bestandteil in der
Diagnostik dar, nicht nur, da sie als nicht ionisierende Bildgebung den
Patienten keiner Röntgenstrahlung aussetzt, sondern auch, da sie in
ihrer Darstellung des Weichgewebes röntgenologischen Untersuchungen
überlegen ist. Obgleich von der MRT aufgrund der strahlenfreien
Bildgebung keine direkten Gefahren für den Patienten ausgehen, bedingt
das statische und das hochfrequente Magnetfeld im MRT dennoch die Beachtung
verschiedener Aspekte im Sinne der Patientensicherheit. Zahnärzten und
Kieferorthopäden sind bei Entscheidungen mitbeteiligt, da sie
häufig bezüglich der vom Patienten getragenen Restaurationen und
Apparaturen konsultiert werden. Aus kieferorthopädischer Sicht stehen
Retainer hierbei im Fokus, da sie anders als alle anderen
kieferorthopädischen Apparaturen im Regelfall ein Leben lang in situ
verbleiben und damit die MRT-Bildgebung bis ins hohe Alter limitieren
können.Wie die meisten dentalen Apparaturen bzw. Restaurationen bestehen nahezu alle
Retainer aus ferromagnetischen Werkstoffen, welche sich durch das zur
MRT-Bildgebung verwendete Hochfrequenzfeld erwärmen können.
Aufgrund des statischen Magnetfelds wirken auf Retainer darüber hinaus
sowohl Rotations- als auch Translationskräfte, über deren
Ausmaß und Auswirkung auf die Patientensicherheit oft Unklarheit bei
vielen Zahnärzten besteht. Neben beiden sicherheitsrelevanten Aspekten
gilt zu beachten, dass Retainer, wie alle anderen metallischen
kieferorthopädischen Apparaturen, Artefakte in der MRT verursachen.
Diese können die Bildqualität erheblich limitieren und eine
Diagnose erschweren.Der folgende Artikel umfasst einen Überblick über die relevanten
Punkte, die es vor der Entscheidung über eine eventuelle Entfernung des
jeweiligen Retainers bei einer MRT-Aufnahme zu beachten gilt.
Influence of Metabolic Syndrome on the Long-Term Prognosis of
Patients with Myocardial Infarction: A Meta-Analysis
Authors: Chen Tianfeng , Shi Zhewei , Qian Caizhen 
Published: December 2023

AbstractThe influence of metabolic syndrome (MetS) on long-term prognosis of patients
with myocardial infarction (MI), the most severe type of coronary artery
disease, remains not fully determined. This systematic review and meta-analysis
were conducted to investigate the association between MetS and long-term
clinical outcomes of patients with MI. A systematic search of Medline, Web of
Science, and Embase databases from inception to June 25, 2023, was conducted to
obtain eligible studies. Only studies with follow-up duration for at least one
year were considered. A random-effects model was utilized to pool the results,
accounting for heterogeneity. Ten observational studies were included, which
included 33 197 patients with MI. Among them, 17 244 (51.9%) were with
MetS at baseline. During a follow-up duration of 12 to 48 months (mean: 22.5
months), patients with MetS were associated with higher incidence of major
adverse cardiovascular events [risk ratio (RR): 1.35. 95% confidence
interval (CI): 1.19 to 1.54, p<0.001; I2=64%] and
all-cause deaths (RR: 1.34, 95% CI: 1.18 to 1.52, p<0.001;
I2=23%), as compared to those without MetS at baseline. Subgroup
analyses showed that the results were not significantly affected by study
characteristics such as study country, design, type of MI, mean age of the
patients, treatment with percutaneous coronary intervention, follow-up
durations, or study quality scores (p for subgroup difference all>0.05).
In patients with MI, MetS may be a risk factor of poor long-term prognosis.
Stereoselective Construction of Vicinal Quaternary Stereocenters via the 1,6-Conjugate Addition of β,β-Disubstituted N-Sulfinyl Metalloenamines to Isatin-Derived para-Quinone Methides
Authors: Lu Chong-Dao , Li Zheng-Fei 
Published: December 2023

AbstractA 1,6-conjugate addition reaction of β,β-disubstituted N-tert-butanesulfinyl metalloenamines, generated via stereospecific α-deprotonation of enantioenriched α,α-disubstituted ketimines or deprotonation of the NH in geometry-defined enesulfinamides, with isatin-derived para-quinone methides is developed to build vicinal quaternary stereocenters with high stereocontrol. The resulting 3,3-disubstituted oxindoles contain a less-accessible acyclic quaternary stereogenic carbon substituted with two sterically and electronically similar groups at the α-position of the imino group. This protocol constitutes a rare example of the stereoselective construction of vicinal quaternary stereocenters via the conjugate addition of acyclic enolates/aza-enolates bearing two similar β-substituents.
Grand Multiparity and Obstetric Outcomes in a Contemporary Cohort: The Role of Increasing Parity
Authors: DeBolt Chelsea A. , Rao Manasa G. , Limaye Meghana A. , London Viktoriya , Sagaram Deepika , Roman Ashley S. , Minkoff Howard , Bernstein Peter S. , Overbey Jessica R. , Kaplowitz Elianna , Meislin Rachel , Toner Lorraine E. , Khander Amrin , Bigelow Catherine A. , Stone Joanne 
Published: December 2023

Objective Evidence is inconsistent regarding grand multiparity and its association with adverse obstetric outcomes. Few large American cohorts of grand multiparas have been studied. We assessed if increasing parity among grand multiparas is associated with increased odds of adverse perinatal outcomes.
Study Design Multicenter retrospective cohort of patients with parity ≥ 5 who delivered a singleton gestation in New York City from 2011 to 2019. Outcomes included postpartum hemorrhage, preterm delivery, hypertensive disorders of pregnancy, shoulder dystocia, birth weight > 4,000 and <2,500 g, and neonatal intensive care unit (NICU) admission. Parity was analyzed continuously, and multivariate analysis determined if increasing parity and other obstetric variables were associated with each adverse outcome.
Results There were 2,496 patients who met inclusion criteria. Increasing parity among grand multiparas was not associated with any of the prespecified adverse outcomes. Odds of postpartum hemorrhage increased with history (adjusted odds ratio [aOR]: 2.65, 95% confidence interval [1.83, 3.84]) and current cesarean delivery (aOR: 4.59 [3.40, 6.18]). Preterm delivery was associated with history (aOR: 12.36 [8.70–17.58]) and non-White race (aOR: 1.90 [1.27, 2.84]). Odds of shoulder dystocia increased with history (aOR: 5.89 [3.22, 10.79]) and birth weight > 4,000 g (aOR: 9.94 [6.32, 15.65]). Birth weight > 4,000 g was associated with maternal obesity (aOR: 2.92 [2.22, 3.84]). Birth weight < 2,500 g was associated with advanced maternal age (aOR: 1.69 [1.15, 2.48]), chronic hypertension (aOR: 2.45 [1.32, 4.53]), and non-White race (aOR: 2.47 [1.66, 3.68]). Odds of hypertensive disorders of pregnancy increased with advanced maternal age (aOR: 1.79 [1.25, 2.56]), history (aOR: 10.09 [6.77–15.04]), and non-White race (aOR: 2.79 [1.95, 4.00]). NICU admission was associated with advanced maternal age (aOR: 1.47 [1.06, 2.02]) and non-White race (aOR: 2.57 [1.84, 3.58]).
Conclusion Among grand multiparous patients, the risk factor for adverse maternal, obstetric, and neonatal outcomes appears to be occurrence of those adverse events in a prior pregnancy and not increasing parity itself.
Key Points
Hemoglobin on Admission for Childbirth and Postpartum Acute Care Use in a Southeastern Health Care System
Authors: Bruce Katharine E. , Busse Clara E. , Tully Kristin P. , Patterson Emily S. , Stuebe Alison M. 
Published: December 2023

Objective Our objective was to evaluate whether hemoglobin on admission for childbirth is associated with postpartum acute care use (ACU).
Study Design We conducted a retrospective cohort study of patients giving birth at a southeastern quaternary care hospital from January 2018 through June 2021 using electronic health records. Predelivery hemoglobin was categorized as <9, 9– < 10, 10– < 11, 11– < 12, and ≥12 g/dL. Acute care was defined as a visit to obstetric triage, the emergency department, or inpatient admission within 90 days postpartum. Generalized estimating equations quantified the crude and multivariable-adjusted association between predelivery hemoglobin and ACU.
Results Among 8,677 pregnancies, 1,467 (17%) used acute care in the system within 90 days postpartum. In unadjusted models, those with predelivery hemoglobin <9 had twice the risk of postpartum ACU compared to those with hemoglobin ≥12 (95% confidence interval [CI]: 1.59–2.69), with a decrease in risk for each increase in hemoglobin category (9– < 10 g/dL: risk ratio [RR]: 1.47, CI: 1.21–1.79; 10– < 11 g/dL: RR: 1.44, CI: 1.26–1.64; 11– < 12 g/dL: RR: 1.20, CI: 1.07–1.34). The adjusted model showed a similar trend with smaller effect estimates (<9 g/dL: RR: 1.50, CI: 1.14–1.98; 9– < 10 g/dL: RR: 1.22, CI: 1.00–1.48; 10– < 11 g/dL: RR: 1.22, CI: 1.07–1.40; 11– < 12 g/dL: RR: 1.09, CI: 0.98–1.22).
Conclusion Low hemoglobin at childbirth admission was associated with increased postpartum ACU. Low hemoglobin on admission could signal to providers that additional follow-up, resources, and ongoing support are warranted to identify and address underlying health needs. Because hemoglobin is routinely assessed during the childbirth hospitalization, this indicator may be especially valuable for risk assessment among patients with limited prior engagement in health care.
Key Points