bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ensuring the competitiveness of regional enterprises on an innovative basis
Authors: Klius Y.I. , Syvochka V.V. 
Published: October 2023

National and regional innovation systems are being formed in Ukraine. In the era of global transformations, innovations become the main factor in increasing the efficiency of industrial enterprises of the region in order to ensure its competitiveness. The solution to this problem requires a comprehensive analysis and rethinking of existing approaches, the creation of theoretical foundations for ensuring the effective development of industrial enterprises in the region. The activity of corporate structures in creating new innovations and effective communications for their transfer becomes a factor of economic development, increasing the competitiveness of the region. The cost of modern products is determined not only by the costs of material production, but also by research and development works, which, in turn, can be an innovative component of the development of industrial enterprises. The use of innovations makes it possible to increase not only the efficiency of individual components of the corporate structure, but also, if proper attention is paid to integration processes, of the entire corporate management system. This determines the increased interest of society, in particular scientists and practitioners, in the ways of effective formation of the corporate innovation management system of an industrial enterprise.
The following tools can be used to stimulate the modernization of industry in order to ensure the competitiveness of the region on the basis of innovations: the formation of the sales market for competitive products and services using mechanisms that regulate the purchase of domestic science-intensive products for state needs; stimulation of investments in the modernization of industry based on innovations by improving the mechanisms of partial financing of large innovative projects by the state and private business; motivation of enterprises to adopt innovations on the basis of incentives for domestic enterprises to purchase modern technologies created by Ukrainian developers; stimulation of industrial reform processes; creation of corporate structures that unite the spheres of production and scientific and technical activity. Among them are state centers of science and high technologies.
Further development of the country's national innovation system should be aimed, first of all, at ensuring the system integration of all its elements and their effective interaction.
Analisis titik impas dan kelayakan usaha ternak ayam petelur mandiri di Kecamatan Way Jepara, Kabupaten Lampung Timur
Authors: Mandala Wintari , Ivan’s Eny 
Published: October 2023

Usaha ternak ayam banyak dibudidayakan dengan pola mandiri oleh masyarakat pedesaan. Kegiatan beternak ayam dilakukan untuk memenuhi kebutuhan pangan sendiri khususnya memenuhi kebutuhan protein hewani. Tujuan dari penelitian adalah untuk menganalisis kelayakan suatu usaha dan titik impas usaha ternak ayam dengan pola mandiri di Desa Braja Dewa, Kecamatan Way Jepara, Lampung Timur. Penelitian ini menggunakan metode survei. Analisis data dilakukan dengan cara menghitung nilai Titik Impas dan R/C ratio. Dari hasil penelitian diperoleh bahwa (1) Nilai BEP produk yang didapat peternak sebesar 6.456 kg per tahun dan berada diatas BEP produksi yakni sebesar 4.585 kg dalam satu tahun; (2) Nilai kelayakan usaha ternak yang didapat sebesar 1,16 > 1. Hasil ini meperlihatkan bahwa usaha ternak ayam petelur ini layak dijalankan.
Many chicken farms are cultivated independently. The activity of raising chickens is carried out to meet their own food needs, especially to meet the needs of animal protein. The purpose of the study was to analyze the feasibility of a business and the break-even point of a chicken farming business with an independent pattern in Braja Dewa Village, Way Jepara District, East Lampung. The research method uses a survey method. Data analysis was carried out by calculating the break-even point and R/C ratio. From the results of the study, it was found that (1) the BEP value of the product obtained by farmers was 6,456 kg per year and was above the production BEP, which was 4,585 kg in one year; (2) The feasibility value of the livestock business obtained is 1,16 > 1. The results prove that the laying hens business is feasible to run.
Employee Branding Pada Aplikasi Sistem Informasi Manajemen Kepegawaian ASN Data Elektronik Mandiri
Authors: Dowa Pretty Petronella Clarita , Uluputty Irfan 
Published: October 2023

Salah satu strategi yang diambil pemerintah dalam bersaing pada era transformasi adalah melalui citra atau reputasi yang dibangun dengan baik bagi publik melalui brand yang dimiliki sesuai tren yang saat ini berkembang yakni employee branding. Fenomena employee branding belum banyak diterapkan pada organisasi pemerintahan, sebagian besar baru pada organisasi profit. Diskominfo Kota Kupang berhasil meningkatkan citra pegawai melalui brand aplikasi SIMPEG ADEM yang dikelola secara mandiri. Penghargaan yang diberikan BKN Awards menunjukkan kemampuan pegawai untuk mewujudkan tujuan organisasi. Tujuan dari penelitian ini adalah membantu untuk menganalisis deskripsi proses employee branding yang diadopsikan pada aplikasi SIMPEG ADEM yang dikembangkan oleh Diskominfo Kota Kupang. Konsep ini dijabarkan oleh empat dimensi yakni bentuk komunikasi, persepsi pegawai, intepretasi aplikasi, dan konsekuensi proses. Desain deskriptif dengan metode kualitatif digunakan untuk bisa menganalisis data penelitian dari hasil wawancara, observasi dan dokumen yang relevan dengan konsep. Kebenaran atas hasil penelitian diuji melalui triangulasi data. Gambaran employee branding pada aplikasi SIMPEG ADEM menunjukkan: 1) komunikasi yang dibangun secara rutin dan baik dalam pelaksanaan tugas; 2) persepsi pegawai terbentuk melalui kegiatan-kegiatan yang diselenggarakan; 3) kemudahan dalam pengaksesan secara mandiri; dan 4) keberhasilan memperoleh penghargaan meski survei belum dilaksanakan. Proses yang telah dijalankan membantu peneliti menemukan bahwa organisasi pemerintah daerah juga dapat menerapkannya dengan mengadopsikannya pada teknologi aplikasi yang dikelola pemerintah.
Kata Kunci: Diskominfo, Employee branding, Pegawai.
Methods for cell isolation and analysis of the highly regenerative tunicate Polycarpa mytiligera
Authors: Gordon Tal , Hendin Noam , Wurtzel Omri 
Published: October 2023

Background:Polycarpa mytiligera is the only molecularly characterized solitary ascidian capable of regenerating all organs and tissue types. The cellular basis for regeneration in P. mytiligera is largely unknown, and methods for isolating live cells from this species for functional analyses are unavailable.Results: Here, we developed a method for isolating live cells from P. mytiligera, overcoming major experimental challenges, including the dissociation of its thick body wall and native cellular autofluorescence. We demonstrated the applicability of our approach for tissue dissociation and cell analysis using three flow cytometry platforms, and by using broadly used non-species-specific cell labeling reagents. In addition to live cell isolation, proof-of-concept experiments showed that this approach was compatible with gene expression analysis of RNA extracted from the isolated cells, and with ex vivo analysis of phagocytosis.Conclusion: We presented efficient methods for cell purification from a highly regenerative ascidian, which could be transferable to diversity of non-model marine organisms. The ability to purify live cells will promote future studies of cell function in P. mytiligera regeneration.
In silico and in vitro study of bioactive compounds of Nigella sativa for targeting neuropilins in breast cancer
Authors: Zafar Imran , Safder Arfa , Imran Afridi Hassan , Riaz Sania , -ur-Rehman Rizwan , Unar Ahsanullah , Un Nisa Fakhar , Gaafar Abdel-Rhman Z. , Bourhia Mohammed , Wondmie Gezahign Fentahun , Sharma Rohit , Kumar Dileep 
Published: October 2023

Introduction: Breast cancer poses a significant global challenge, prompting researchers to explore novel approaches for potential treatments.Material and Methods: For in vitro study we used thin layer chromatography (TAC) for phytochemical screening, total antioxidant capacity (TLC) assay for antioxidant capacity, and hemolytic activity test for toxicity of Neuropilins (NRPs). We performed bioinformatic analyses to predict protein structures, molecular docking, pharmacophore modeling, and virtual screening to reveal interactions with oncogenes. We conducted 200 ns Molecular Dynamics (MD) simulations and MMGBSA calculations to assess the complex dynamics and stability.Results: We identified phytochemical constituents in Nigella sativa leaves, including tannins, saponins, steroids, and cardiac glycosides, while phlobatannins and terpenoids were absent. The leaves contained 9.4% ± 0.04% alkaloids and 1.9% ± 0.05% saponins. Methanol extract exhibited the highest yield and antioxidant capacity, with Total Flavonoid Content at 127.51 ± 0.76 mg/100 g and Total Phenolic Content at 134.39 ± 0.589 mg GAE/100 g. Hemolysis testing showed varying degrees of hemolysis for different extracts. In-silico analysis indicated stable Neuropilin complexes with key signaling pathways relevant for anti-cancer therapy. Molecular docking scores at different possesses (0, C-50, C −80, C-120,C −150, C −200 ns) revealed strong hydrogen bonding in the complexes and showed −12.9, −11.6, and −11.2 binding Affinities (kcal/mol) to support their stability. Our MD simulations analysis at 200ns confirmed the stability of Neuropilin complexes with the signaling pathways protein PI3K. The calculated binding free energies using MMGBSA provided valuable quantitative information on ligand potency on different time steps. These findings highlight the potential health benefits of N. sativa leaves and their possible role in anti-cancer treatments targeting angiogenesis.Conclusion:Nigella sativa leaves have shown significant medical potential due to their bioactive compounds, which exhibit strong properties in supporting organogenic processes related to cancer. Furthermore, studies have highlighted the promising role of neuropilins in anticancer treatment, demonstrating stable interactions and potential as targeted therapy specifically for breast cancer.
Artificial intelligence-enhanced 12-lead electrocardiography for identifying atrial fibrillation during sinus rhythm (AIAFib) trial: protocol for a multicenter retrospective study
Authors: Baek Yong-Soo , Kwon Soonil , You Seng Chan , Lee Kwang-No , Yu Hee Tae , Lee So-Ryung , Roh Seung-Young , Kim Dong-Hyeok , Shin Seung Yong , Lee Dae In , Park Junbeom , Park Yae Min , Suh Young Ju , Choi Eue-Keun , Lee Sang-Chul , Joung Boyoung , Choi Wonik , Kim Dae-Hyeok 
Published: October 2023

IntroductionAtrial fibrillation (AF) is the most common arrhythmia, contributing significantly to morbidity and mortality. In a previous study, we developed a deep neural network for predicting paroxysmal atrial fibrillation (PAF) during sinus rhythm (SR) using digital data from standard 12-lead electrocardiography (ECG). The primary aim of this study is to validate an existing artificial intelligence (AI)-enhanced ECG algorithm for predicting PAF in a multicenter tertiary hospital. The secondary objective is to investigate whether the AI-enhanced ECG is associated with AF-related clinical outcomes.Methods and analysisWe will conduct a retrospective cohort study of more than 50,000 12-lead ECGs from November 1, 2012, to December 31, 2021, at 10 Korean University Hospitals. Data will be collected from patient records, including baseline demographics, comorbidities, laboratory findings, echocardiographic findings, hospitalizations, and related procedural outcomes, such as AF ablation and mortality. De-identification of ECG data through data encryption and anonymization will be conducted and the data will be analyzed using the AI algorithm previously developed for AF prediction. An area under the receiver operating characteristic curve will be created to test and validate the datasets and assess the AI-enabled ECGs acquired during the sinus rhythm to determine whether AF is present. Kaplan–Meier survival functions will be used to estimate the time to hospitalization, AF-related procedure outcomes, and mortality, with log-rank tests to compare patients with low and high risk of AF by AI. Multivariate Cox proportional hazards regression will estimate the effect of AI-enhanced ECG multimorbidity on clinical outcomes after stratifying patients by AF probability by AI.DiscussionThis study will advance PAF prediction based on AI-enhanced ECGs. This approach is a novel method for risk stratification and emphasizes shared decision-making for early detection and management of patients with newly diagnosed AF. The results may revolutionize PAF management and unveil the wider potential of AI in predicting and managing cardiovascular diseases.Ethics and disseminationThe study findings will be published in peer-reviewed publications and disseminated at national and international conferences and through social media. This study was approved by the institutional review boards of all participating university hospitals. Data extraction, storage, and management were approved by the data review committees of all institutions.
Clinical Trial Registration[cris.nih.go.kr], identifier (KCT0007881).
Serum uric acid is related to liver and kidney disease and 12-year mortality risk after myocardial infarction
Authors: Heerkens Luc , van Westing Anniek C. , Voortman Trudy , Kardys Isabella , Boersma Eric , Geleijnse Johanna M. 
Published: October 2023

ObjectiveTo study the associations of non-alcoholic fatty liver disease (NAFLD), chronic kidney disease (CKD), and serum uric acid (SUA) in patients with post–myocardial infarction (MI) patients, and the relationship of SUA with 12-year mortality risk.MethodsWe included 3,396 patients (60–80 years old, 78% men) of the Alpha Omega Cohort. Multivariable prevalence ratios (PRs) were obtained for the association of NAFLD [fatty liver index (FLI), ≥77 (women) and ≥79 (men)] with CKD [estimated glomerular filtration rate (eGFR), &lt;60 mL/min per 1.73 m2]. We calculated sensitivity and specificity of SUA to detect the (combined) presence and absence of NAFLD and CKD. Cause-specific mortality was monitored from enrolment (2002–2006) through December 2018. Hazard ratios (HRs) for all-cause and cardiovascular disease (CVD) mortality in SUA categories were obtained from multivariable Cox models.ResultsMedian baseline FLI was 67 (men, 68; women, 64), and mean ± SD eGFR was 81 ± 20 mL/min per 1.73 m2 (17% with CKD). Sex-specific FLI was associated with higher CKD prevalence (PRtertile3 vs. tertile1, 1.94; 95% confidence interval: 1.57, 2.39). Baseline SUA was 0.36 ± 0.09 mmol/L. With increasing SUA concentrations, specificity for the presence of NAFLD, CKD, or both increased, and sensitivity decreased. During 12 (interquartile range, 9–14) years of follow-up, 1,592 patients died (713 from CVD). HRs ranged from 1.08 (0.88, 1.32) for SUA ≤0.25 mmol/L to 2.13 (1.75, 2.60) for SUA &gt;0.50 mmol/L vs. SUA &gt;0.30–0.35 mmol/L for all-cause mortality. For CVD mortality, HRs ranged from 1.05 (0.77, 1.44) to 2.43 (1.83, 3.25).ConclusionsNAFLD and CKD were strongly associated, which was reflected by higher SUA concentrations. SUA was a strong predictor of 12-year mortality risk after MI.
Preoperative and postoperative blood testosterone levels in patients with acromegaly: a prospective study
Authors: Zhang Duoxing , Guo Xiaopeng , Feng Ming , Bao Xinjie , Deng Kan , Yao Yong , Lian Wei , Xing Bing , Wang Hanbi 
Published: October 2023

PurposeTo investigate the prevalence of low blood testosterone level (LTL) and its determinant factors among active male acromegaly patients, as well as the effect of surgery on LTL in male acromegaly patients.MethodsA retrospective, single-center study focused on 252 male acromegaly patients aged 18 years–60 years diagnosed in the Peking Union Medical College Hospital from January 2015 to December 2018 was carried out. The measurements of preoperative and postoperative testosterone levels, serum growth hormone (GH), insulin-like growth factor 1 (IGF-1), and other clinical data were analyzed.ResultsForty per cent of subjects included were diagnosed with LTL pre surgery. Patients were divided into normal testosterone level (NTL) and LTL groups based on their testosterone level. There were significant differences (p &lt; 0.01) between groups in the presence of macroadenomas, invasion of the cavernous sinus, compression of the optic chiasm, and serum GH and prolactin levels pre surgery. Invasion of the cavernous sinus [odds ratio (OR) = 4.299; p = 0.000] and serum prolactin level (OR = 1.023, p = 0.001) were independent predictors of LTLs in male patients before surgical intervention. A total of 67.9% of LTL patients recovered during the follow-up, with a new-onset rate of 3.4%. Body mass index, invasion of the cavernous sinus, GH, IGF-1, and prolactin levels, the presence of a prolactin-secreting tumor, and recovery from acromegaly were significantly different (p &lt; 0.05) in the NTL group and in the LTL group during the follow-up. The presence of a prolactin-secreting tumor (OR = 0.224; p = 0.001) and recovery from acromegaly (OR = 0.168; p = 0.006) were independent predictors of LTLs in male acromegaly patients during the follow-up.ConclusionThe invasiveness of tumor and levels of blood prolactin are independent factors for LTLs before surgery, whereas GH and IGF-1 levels are not. Most male patients can recover from LTL after tumor restriction surgery: those who recover from acromegaly have a better chance of recovering from LTL.
Circulating donor-derived cell-free DNA as a marker for rejection after lung transplantation
Authors: Li Yunhui , Liang Bin 
Published: October 2023

ObjectiveRecently, circulating donor-derive cell free DNA (dd-cfDNA) has gained growing attention in the field of solid organ transplantation. The aim of the study was to analyze circulating dd-cfDNA levels in graft rejection, ACR and AMR separately for each rejection type compared with non-rejection, and assessed the diagnostic potential of dd-cfDNA levels in predicting graft rejection after lung transplantation.MethodsA systematic search for relevant articles was conducted on Medline, Web of Science, China National Knowledge Infrastructure (CNKI), and Wanfang databases without restriction of languages. The search date ended on June 1, 2023. STATA software was used to analyze the difference between graft rejection, ACR, AMR and stable controls, and evaluate the diagnostic performance of circulating dd-cfDNA in detecting graft rejection.ResultsThe results indicated that circulating dd-cfDNA levels in graft rejection, ACR, and AMR were significantly higher than non-rejection (graft rejection: SMD=1.78, 95% CI: 1.31-2.25, I2 = 88.6%, P&lt; 0.001; ACR: SMD=1.03, 95% CI: 0.47-1.59, I2 = 89.0%, P &lt; 0.001; AMR: SMD= 1.78, 95% CI: 1.20-2.35, I2 = 89.8%, P &lt; 0.001). Circulating dd-cfDNA levels distinguished graft rejection from non-rejection with a pooled sensitivity of 0.87 (95% CI: 0.80-0.92) and a pooled specificity of 0.82 (95% CI: 0.76-0.86). The corresponding SROC yield an AUROC of 0.90 (95% CI: 0.87-0.93).ConclusionCirculating dd-cfDNA could be used as a non-invasive biomarker to distinguish the patients with graft rejection from normal stable controls.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42023440467.
Evaluating the impact of testing strategies for the detection of nosocomial COVID-19 in English hospitals through data-driven modeling
Authors: Evans Stephanie , Stimson James , Pople Diane , Wilcox Mark H. , Hope Russell , Robotham Julie V. 
Published: October 2023

IntroductionDuring the first wave of the COVID-19 pandemic 293,204 inpatients in England tested positive for SARS-CoV-2. It is estimated that 1% of these cases were hospital-associated using European centre for disease prevention and control (ECDC) and Public Health England (PHE) definitions. Guidelines for preventing the spread of SARS-CoV-2 in hospitals have developed over time but the effectiveness and efficiency of testing strategies for preventing nosocomial transmission has not been explored.MethodsUsing an individual-based model, parameterised using multiple datasets, we simulated the transmission of SARS-CoV-2 to patients and healthcare workers between March and August 2020 and evaluated the efficacy of different testing strategies. These strategies were: 0) Testing only symptomatic patients on admission; 1) Testing all patients on admission; 2) Testing all patients on admission and again between days 5 and 7, and 3) Testing all patients on admission, and again at days 3, and 5-7. In addition to admissions testing, patients that develop a symptomatic infection while in hospital were tested under all strategies. We evaluated the impact of testing strategy, test characteristics and hospital-related factors on the number of nosocomial patient infections.ResultsModelling suggests that 84.6% (95% CI: 84.3, 84.7) of community-acquired and 40.8% (40.3, 41.3) of hospital-associated SARS-CoV-2 infections are detectable before a patient is discharged from hospital. Testing all patients on admission and retesting after 3 or 5 days increases the proportion of nosocomial cases detected by 9.2%. Adding discharge testing increases detection by a further 1.5% (relative increase). Increasing occupancy rates, number of beds per bay, or the proportion of admissions wrongly suspected of having COVID-19 on admission and therefore incorrectly cohorted with COVID-19 patients, increases the rate of nosocomial transmission. Over 30,000 patients in England could have been discharged while incubating a non-detected SARS-CoV-2 infection during the first wave of the COVID-19 pandemic, of which 3.3% could have been identified by discharge screening. There was no significant difference in the rates of nosocomial transmission between testing strategies or when the turnaround time of the test was increased.DiscussionThis study provides insight into the efficacy of testing strategies in a period unbiased by vaccines and variants. The findings are relevant as testing programs for SARS-CoV-2 are scaled back, and possibly if a new vaccine escaping variant emerges.