bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxia promotes metastasis by relieving miR-598-3p-restricted glycolysis in gastric cancer
Authors: Zhou Wei , Tang Mengyuan , He Dan , Wu Zhiyun , Shen Yi , Wei Wenxiang , Zheng Hui , Wang Qi , Shi Weifeng , Jiang Jingting 
Published: October 2023

Abstract
The activation of glycolysis, particularly in the context of reprogrammed energy metabolism, is increasingly recognized as a significant characteristic of cancer. However, the precise mechanisms by which glycolysis is promoted in metastatic gastric cancer cells under normal oxygen conditions remain poorly understood. MicroRNAs (miRNAs) play a crucial role in the development of malignant phenotypes in gastric cancer. Nevertheless, our understanding of the specific involvement of miRNAs in hypoxia-induced metabolic shifts and the subsequent metastatic processes is limited. Hypoxia-induced downregulation of miR-598-3p mechanistically leads to the upregulation of RMP and IGF1r, thereby promoting glycolysis through the upregulation of multiple glycolysis-related genes. Our findings demonstrate that inhibiting glycolysis through either overexpression of miR-598-3p or R406 treatment effectively suppresses the metastasis of gastric cancer cells both in vitro and in vivo. Collectively, the depletion of miR-598-3p alters glucose metabolism from oxidative phosphorylation to glycolysis, potentially exacerbating the malignancy of gastric cancer cells. The present findings indicate a potential target for the development of therapeutics against gastric cancers with increased miR-598-3p expression.
Safety and efficacy of overlapping triangular anastomoses in complete laparoscopic colorectal resection: a systematic evaluation and Meta-analysis
Authors: Li Junjing , Shao Lei , Cai Baojia , Zhu Shengmao , Han Jingjun , Li Jintao , Wang Kaimin , Zhang Chengwu , Yu Pengjie 
Published: October 2023

Abstract
Background
To systematically evaluate and compare the safety and efficacy of overlapping triangular anastomoses and other anastomoses in total laparoscopic colorectal resection for the treatment of colorectal cancer, and to provide evidence-based medical evidence for clinical practice.
Materials and methods
PubMed, Embase, Cochrane Library and Web of Science databases were searched until May 2023, published literature related to total laparoscopic overlapping triangular anastomosis colorectal resection versus colorectal resection with other anastomoses was obtained, progressively screened, and eligible studies were included, and finally Meta-analysis was performed by Stata17 software.
Results
Seven literatures were included. 705 patients were included, including 342 patients who received overlapping triangular anastomoses and 363 patients who received other anastomoses. A meta-analysis of the included metrics showed that overlapping triangular anastomoses and other anastomoses were more effective in terms of operative time (SMD=-0.29, 95%CI-0.82, 0.23, P = 0.278), postoperative exhaust time (SMD=-0.61, 95%CI-0.84,-0.37), P = 0.000), intraoperative blood loss (SMD=-0.06, 95% CI-0.21,0.10, P = 0.493), length of hospital stay (SMD=-0.41, 95% CI-0.86,0.05, P = 0.079), incision length (SMD=-0.70, 95%CI-1.22,-0.19, P = 0.007), anastomosis time (SMD=-0.56, 95%CI-1.09,-0.03, P = 0.040), ground time (SMD=-0.33, 95%CI-0.65,-0.01, P = 0.041), postoperative defecation time (SMD=-0.75, 95%CI-1.39,-0.10, P = 0.023), number of lymph nodes dissection (SMD = 0.21, 95%CI-0.01,0.43, P = 0.063). From the results, it is clear that there was no significant difference between the overlapping triangular anastomosis and other anastomoses in terms of operative time, intraoperative bleeding, length of hospital stay, and number of lymph nodes discharged (P > 0.05). Compared with other anastomoses, the overlapping triangular anastomosis showed a significant difference in terms of shorter postoperative venting time, smaller incision length, shorter anastomosis time, earlier time on the floor, and earlier postoperative defecation time (P < 0.05).
Conclusion
The overlapping triangular anastomosis in complete laparoscopic colorectal resection is safe and effective, and is superior to other anastomoses in terms of postoperative venting time, incision length, and postoperative defecation time.
Exploring Brain PET Connectivity Data with Machine Learning: A Case Study on Primary Progressive Aphasia
Authors: Fernando García-Gutiérrez , Laura Hernández-Lorenzo , María Nieves Cabrera-Martín , Lucía Fernández-Romero , A. Matias-Guiu Jordi , L. Ayala José 
Published: November 2023

Abstract
Graph Theory has spread across different domains due to its ability to capture and exploit information about the interactions among elements. One of the most promising applications relies on the area of Neuroscience, where the exploration of brain connectivity (BC) has become a topic of major interest. In this context, we introduce a new framework to extract and analyze BC data for detecting disrupted connectivity subnetworks associated with neurodegenerative disorders. Our framework conceptualizes the problem of identifying connectivity subnetworks as an optimization, and leverages the potential of Machine Learning techniques to optimize the objective function. We applied this methodology to 18F-fluorodeoxyglucose positron emission tomography (FDG-PET) data from patients with primary progressive aphasia (PPA), a neurodegenerative syndrome characterized by three distinct clinico-pathological variants featuring diverse topographical and horological patterns. Our findings showed that the proposed approach detects impaired BC subnetworks associated with the characteristic fingerprints of the disease. This methodology offers a fresh perspective on studying BC, complementing conventional approaches based on Graph Theory.
Stem cell membrane-cloaked biomimetic nanoparticles targeted delivery of Naringin to control cytokine storm in mouse pneumonia
Authors: jin hua , Zhao Yue , Ouyang Suidong , Yao Yinlian , Fan Shilong , Luo Renxing , Pi Jiang , Wang Yanyan , Huang Gonghua 
Published: October 2023

Abstract
Background:
Cytokine storm or cytokine release syndrome (CRS), mainly caused by the bacteria, viruses, etc., is life-threatening systemic inflammatory syndrome. CRS in the lung can lead to acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) in a very short time, and it is the main reason for patients with mild symptoms transferring to severe disease even death. Thus, effectively controlling CRS is essential in the prevention and treatment of infectious pneumonia.
Methods:
The naringin nanoparticles (Nar-NPs) were prepared by emulsification-and-evaporation method, and then the mesenchymal stem cell membranes were extracted and coated onto the surface of Nar-NPs by hand extrusion through nano-porous membranes to obtain cell membrane (CM) cloaked CM@Nar-NPs. The biocompatibility, inflammation-targeting, antioxidant capacity and anti-inflammatory efficiency were determined both in vitro and in vivo.
Results
The as-synthesized biomimetic platform (CM@Nar-NPs) showed highly efficient targeting and Nar delivery to the macrophages and alveolar epithelial cells as demonstrated by fluorescent imaging and flow cytometry analyses. The lung inflammation-targeting and accumulation of CM@Nar-NPs were also confirmed by IVIS imaging in septic mice. In LPS-induced sepsis mouse model, CM@Nar-NPs showed efficient therapeutic efficacy, as evidenced by improved survival rate, decreased pulmonary permeability and cytokine release, and the organ damage protection. Additionally, CM@Nar-NPs exhibited excellent antioxidant capacity, as evidenced by ROS scavenging in inflamed RAW264.7 cells and in the lung and liver of septic mice. To further determine the protective mechanism of CM@Nar-NPs, infiltrated macrophages in the lung were isolated and analyzed. CM@Nar-NPs increased the expression of M2 marker (CD206), and decreased the expression of M1 marker (F4/80) in septic mice, suggesting that Nar-modulated macrophages polarizing towards M2 subtype might contribute to their anti-inflammation effects.
Conclusion
The as-synthesized biomimetic platform (CM@Nar-NPs) can be employed to targeted delivery and improve the therapeutic efficacy of active ingredients from herbal medicine in the treatment of inflammatory diseases. This nanoplatform shows potential prospects for pneumonia-targeted delivery in clinics.
Dynamics of Ankylosing Spondylitis-associated Arthritogenic Peptide-MHC I interactions
Authors: Kıvrak Sena , Dilek Yunus Emre , Kara İrem , Özkaya Şeyma Çolakoğlu , Abacar Kerem Yiğit , Erzik Can , Atagündüz Mehmet Pamir , Akçapınar Günseli Bayram 
Published: October 2023

Abstract
Ankylosing spondylitis (AS) is a chronic inflammatory disorder affecting the axial skeleton and often associated with Human Leukocyte Antigen-B*27 (HLA-B*27) positivity. HLA-B*27 and its role in AS pathogenesis remain unclear despite the identification of multiple susceptibility alleles. As the most frequent subtype related to AS, HLA-B*27:05 differs from the non-associated HLA-B*27:09 subtype at a single position. This study focuses on the comparison of two subtypes in their binding to two arthritogenic peptides (ARGQPGVMG-DRASFIKNL) and a viral peptide (KK10) through 500 ns long molecular dynamic simulations. In the present study, it was found that peptide-MHC I complex stability and peptide presentation were similar when the peptides had similar C-terminal charges.
CircFOXO3 Upregulation Mediates the Radioresistance of Glioblastoma by Affecting Cellular Metabolome
Authors: Xu Hao , Xing Jin , Cheng Linlin , wang Zhihan , zhao Liang , Ren Li , Zhang Shuai 
Published: October 2023

Abstract
Radioresistance remains an important barrier to the treatment of glioblastoma multiforme (GBM), which is the most prevalent and lethal brain cancer in adults. Metabolic alterations contribute to radioresistance through various mechanisms, including activation of antioxidant responses to counteract reactive oxygen species (ROS) and DNA repair. In this study, we observed a significant up-regulation of circFOXO3 in glioma cells upon exposure to radiation and recurrent GBM tissues. Knockdown of circFOXO3 enhanced radiosensitivity in glioma cells. An assay of orthotopic GBM animal model in vivo indicated that inhibition of circFOXO3 significantly suppressed GBM progression and prolonged survival time. Overexpression of circFOXO3 significantly attenuated radiosensitivity in glioma cells. Additionally, metabolomics analysis revealed substantial alterations in the metabolomic profiles between the circFOXO3-OE and control groups following irradiation, particularly involving lipids and lipid-like molecules, organic acids and derivatives, and organoheterocyclic compounds. Moreover, suppression of circFOXO3 increased levels of pro-apoptotic proteins Caspase 7 and Bax while decreasing Bcl-2 levels after radiotherapy. Our findings establish the crucial role played by circFOXO3 in tumor radioresistance through modulation of metabolites, highlighting its potential as a diagnostic and therapeutic target for GBM.
Precise Characterization of Differentiated SH-SY5Y Cells Using a Novel APP Splice Variant Dependent Method: for Intended Use in AD Research
Authors: Kulatunga D Chanuka M , Ranaraja Umanthi , Kim Eun Young , Kim Ryoung Eun , Kim Dong Ern , Ji Kuk Bin , Kim Min Kyu 
Published: October 2023

Abstract
SH-SY5Y, a neuroblastoma cell line, can be converted into mature neuronal phenotypes, characterized by the expression of mature neuronal and neurotransmitter markers. However, the mature phenotypes described across multiple studies appear inconsistent. As this cell line expresses common neuronal markers after a simple induction, there is a high chance of misinterpreting its maturity. Therefore, sole reliance on common neuronal markers is presumably inadequate. The Alzheimer's disease (AD) central gene, amyloid precursor protein (APP), has shown contrasting transcript variant dynamics in various cell types. We differentiated SH-SY5Y cells into mature neuron-like cells using a concise protocol and observed the upregulation of total APP throughout differentiation. However, APP transcript variant-1 was upregulated only during the early to middle stages of differentiation and declined in later stages. We identified the maturity state where this post-transcriptional shift occurs, terming it "true maturity." At this stage, we observed a predominant expression of mature neuronal and cholinergic markers, along with a distinct APP variant pattern. Our findings emphasize the necessity of using a differentiation state-sensitive marker system to precisely characterize SH-SY5Y differentiation. Moreover, this study offers an APP-guided, alternative neuronal marker system to enhance the accuracy of the conventional marker system.
Application of Machine Learning for Predicting Brucellosis Disease in Dairy Cattle
Authors: Hussaini S. M.A.K. , Tito M. H. , Hasan M. M. , Rahman Md. Siddiqur , Sharmy S. T. , Sarker R. R. , Rahman A. K. M. A. , Neubauer H. 
Published: November 2023

Abstract
Brucellosis, a zoonotic disease caused by Brucella bacteria, has emerged as a significant concern in Bangladesh, affecting both animals and humans, with economic repercussions and health risks. This paper addresses the urgent need for effective disease management in a country where agriculture and livestock are pivotal to the economy. The study focuses on identifying risk factors for brucellosis in dairy cattle and their correlation with factors collected from Central Cattle Breeding and Dairy Farm (CCBDF) and Military Dairy Farms (MDF) in Bangladesh. Serum and milk samples, along with demographic data, were collected and analyzed. To address the challenge of imbalanced data, the study introduces the application of the "SMOTE" Weka filter for the first time in Bangladesh's veterinary profession. This balances the dataset, enhancing the accuracy of predictive modeling. Machine learning models, specifically Multilayer Perceptron (MLP) and J48 decision tree algorithms, are employed for brucellosis prediction. MLP achieved a Correct Classification (CC) rate of 95.0801%, while J48 achieved 94.0503%, demonstrating their efficacy. Sensitivity analysis identifies key attributes, with retained placenta being the most significant, aiding in prioritizing control measures. Association Rules reveal patterns and relationships among disease-related factors, enhancing understanding and proactive prevention.
A usability study on mobile EMG-guided wrist extension training in subacute stroke patients－MyoGuide
Authors: Lin Hao-Ping , Xu Yang , Zhang Xue , Woolley Daniel , Zhao Lina , Liang Weidi , Huang Mengdi , Cheng Hsiao-ju , Zhang Lixin , Wenderoth Nicole 
Published: October 2023

Abstract
Background: Effective stroke rehabilitation requires high-dose, repetitive-task training, especially during the early recovery phase. However, the usability of upper-limb rehabilitation technology in acute and sub-acute stroke survivors remains relatively unexplored. In this study, we introduce sub-acute stroke survivors to the “MyoGuide”, a mobile training platform that employs surface electromyography (sEMG)-guided neurofeedback training for post-stroke wrist extension. Notably, the study places a strong emphasis on evaluating the platform’s usability within clinical contexts.
Methods: We report the results of seven sub-acute post-stroke participants. The MyoGuide mobile training platform provided participants with real-time feedback, gamification features, and user control. Participants underwent wrist extension training, which encompassed calibration, stability assessment, and dynamic tasks. The training was conducted in supervised 1:1 sessions, spanning ten days. All training records were recorded within the application, usability was assessed through System Usability Scale (SUS) and a questionnaire administered during the final session. Daily written reports were provided by the therapist throughout the study duration.
Results: The usability analysis yielded positive results, with a median SUS score of 82.5. Across the training sessions, patients progressed as indicated by significant increases in both the Stability Assessment Scores and the Level of Difficulty (LoD) that could be achieved in the dynamic task. The rate of progression differed based on initial impairment levels of the patient. During the training sessions, therapists documented not only the day-to-day performance of participants but also the extent of support required, particularly for those with lower baseline motor function. In parallel, participants who had experienced stroke expressed a keen interest in continuing home-based training. However, they also acknowledged challenges related to independently using the Myo armband and software.
Conclusions: This study introduces the MyoGuide training platform and demonstrates its usability in a clinical setting for stroke rehabilitation, with the assistance of a therapist. The findings support the potential of MyoGuide for wrist extension training in patients across a wide range of impairment levels. However, certain usability challenges, such as donning/doffing the armband and navigating the application, need to be addressed to enable independent MyoGuide training requiring only minimal supervision by a therapist.
Impact of COVID-19 restrictions on newly occurring loneliness in older people – a prospective study in a memory clinic population
Authors: Defrancesco Michaela , Hofer Alex , Schurr Timo 
Published: October 2023

Abstract
The COVID-19 pandemic was associated with high burden and numerous negative consequences for older people. Social isolation and loneliness are major and prevalent health problems impacting well-being and quality of life and may have increased due to pandemic-related restrictions. The present study investigate the impact of the COVID-19 pandemic on loneliness in people visiting a memory clinic between March 2020 and September 2022. We conducted a prospective, single-center, questionnaire-based observational follow-up study to assess potential predictors of newly occurring, pandemic-related loneliness. Next to a newly developed COVID-19 questionnaire, a comprehensive neuropsychological test battery, the Neuropsychiatric Inventory and the Geriatric Depression Scale were used. In total 426 people (mean age: 76.48 years, 12.9% cognitively intact, 33.1% diagnosed with Mild Cognitive Impairment, 49.8% diagnosed with dementia, and 4.2% diagnosed with depression) completed the COVID-19 questionnaire at baseline and 166 at follow-up. Newly occurring loneliness was indicated by 22.3% of baseline participants and by 24.1% of follow-up participants. Results of logistic regression analysis showed that living alone (OR 5.452) and having less contact with friends (OR 2.771) were most predictive of the occurrence of loneliness. The use of digital communication media as an alternative strategy for social interaction was lowest in dementia patients (6–13%). In conclusion, personal contacts and a close friendship network appear to be more decisive to prevent loneliness in older people than does the use of digital communication media. However, promoting an intensified use of digital communication media may be useful to counteract loneliness, especially in dementia patients.
Mediating role of perceived social support between frailty and self-perceived burden in older patients with diabetes
Authors: Chen Ming-Zhu , Chen Xiao-Lei , Xu Qin , Cao Xiao-Dong , Wang Meng-Yu , Cao Rong 
Published: October 2023

Abstract
Objective To explore the mediating effects of perceived social support between frailty and self-perceived burden in older patients with diabetes, then provide theoretical basis for reducing the perceived burden of senile diabetic patients. Methods A total of 169 older diabetic patients who were hospitalized in the endocrinology department of a third-class hospital in Wuxi fromMay 2020 to July 2022 were investigated by convenience sampling method. Patients were investigated by the general information questionnaire, the Chinese version of the Tilburg Frailty Inventory (TFI), the Self-Feeling Burden Scale (SPBS), the Perceived Social Support Scale (PSSS).SPSS 22.0 was exployed for Pearson correlation analysis and multiple linear regression analysis, and we selected the Model 4 of SPSS PROCESS for mediating effect analysis. Results The SPBS of senile diabetes mellitus was positively correlated with TFI (P < 0.01), and negatively correlated with PSSS (P < 0.01).The results of Bootstrap test showed that the mediating effect of PSSS on the relationship between TFI and SPBS in older diabetic patients was 0.296(95%CI: 0.007, 0.066), and the mesomeric effect accounted for 17.3% of the total effect. Conclusion The debilitation of older patients with diabetes can reduce the burden of self-perception by perceived social support. Based on the improving perceived support, nurses should actively carry out comprehensive interventions,in order toreducing the self-perceived burden of older patients with diabetes.
Multivariate time Series Collaborative Compression for Monitoring Systems in Securing Cloud-based Digital Twin
Authors: Miao Zicong , Li Weize , Pan Xiaodong 
Published: October 2023

Abstract
With the booming of cloud-based digital twin systems, monitoring key performance indicators has become crucial for ensuring system security and reliability. Due to the massive amount of monitoring data generated, data compression is necessary to save data transmission bandwidth and storage space. Although the existing research has proposed compression methods for multivariate time series (MTS), it is still a challenge to guarantee the correlation between data when compressing the MTS. This paper proposes an MTS Collaborative Compression (MTSCC) method based on the two-step compression scheme. First, shape-based clustering is implemented to group the MTS. Afterward, the compressed sensing is optimized to achieve collaborative compression of grouped data. Based on a real-world MTS dataset, the experimental results show that the proposed MTSCC can effectively preserve the complex temporal correlation between indicators while achieving efficient data compression, and the root mean squared error of correlation between the reconstructed and original data is only 0.0489 in the case of 30% compression ratio. Besides, it is verified that using the reconstructed data in the production environment has almost the same performance as using the original data.