bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sexually concordant selection on floral traits despite greater opportunity for selection through male fitness
Authors: Hou Meng , Opedal Øystein H. , Zhao Zhi‐Gang 
Published: October 2023

Summary
Pollinators are important drivers of floral trait evolution, yet plant populations are not always perfectly adapted to their pollinators. Such apparent maladaptation may result from conflicting selection through male and female sexual functions in hermaphrodites.
We studied sex‐specific mating patterns and phenotypic selection on floral traits in Aconitum gymnandrum. After genotyping 1786 offspring, we partitioned individual fitness into sex‐specific selfed and outcrossed components and estimated phenotypic selection acting through each.
Relative fitness increased with increasing mate number, and more so for male function. This led to greater opportunity for selection through outcrossed male fitness, though patterns of phenotypic selection on floral traits tended to be similar, and with better support for selection through female rather than male fitness components. We detected directional selection through one or more fitness component for larger flower number, larger flowers, and more negative nectar gradients within inflorescences.
Our results are consistent with Bateman's principles for sex‐specific mating patterns and illustrate that, despite the expected difference in opportunity for selection, patterns of variation in selection across traits can be rather similar for the male and female sexual functions. These results shed new light on the effect of sexual selection on the evolution of floral traits.
Association between maternal growth in the pre‐conception and early gestational period of nulliparous dairy heifers with anti‐Müllerian hormone in their female offspring
Authors: Thomson Eilidh E. , Beltman Marijke E. , Crowe Mark A. , McAloon Conor G. , Furlong John G. , Duane Mary M. , Brennan Jane P. N. , McDonald Michael M. , Kelly Emmet T. 
Published: October 2023

AbstractAnti‐Müllerian hormone (AMH) is secreted by granulosa cells of healthy, growing follicles and is positively correlated with the ovarian reserve. Maternal and environmental factors, such as nutrition, disease, parity and endocrine disruptors, are thought to have a profound impact on ovarian reserve development during early foetal life. For genetic progress, it can be advantageous to breed dairy replacements from heifers to expedite the generation interval; however, there is some evidence that nulliparous animals produce female offspring with smaller ovarian reserves compared with multiparous animals. The objective of this prospective, observational study was to determine whether maternal growth in the pre‐conception and early gestational period of nulliparous dairy heifers is associated with pre‐weaning AMH concentrations in their female offspring. Our hypothesis was that excessive growth in this period would negatively impact AMH concentrations. Seasonal, pasture‐based dairy heifer calves (n = 156) born from nulliparous dams, from six Irish farms, were blood sampled at an average of 60 days of age in spring 2022 and tested for AMH. Mixed‐effects linear regression models were constructed with Box‐Cox transformed AMH concentration as the dependent variable. The independent variables tested included maternal average daily gain (ADG) from pre‐breeding examination (PBE) to pregnancy diagnosis (PD) between 30 and 60 days in calf (DIC), ADG from PBE to PD over 60 DIC and ADG between the two PDs. Calf breed and age at sampling were forced into the models, and the farm was treated as a random effect in all models. We found that as ADG increased from the pre‐breeding period to their first PD visit, the AMH concentration in their offspring reduced. However, ADG explained only a small amount of the variation in AMH concentrations (marginal R2 = 0.041). In conclusion, the results of our study suggest that excessive growth prior to conception and in early gestation of nulliparous heifers could impact the ovarian reserve of their female offspring, and may imply that farmers should avoid excessive growth in the immediate pre‐breeding and early gestational periods.
Musical taste preferences in Chinese hip‐hop: Between cultural openness and the rejection of vulgarity
Authors: Wang Yehan 
Published: October 2023

AbstractRecent debates on musical taste have centred on the collapse of cultural boundaries between high art and popular culture. This paper examines Chinese hip‐hop as a unique case study to explore taste preferences in a relatively new popular cultural phenomenon in the understudied context of Eastern popular culture. Drawing from 42 interviews with Chinese young hip‐hop followers, the article examines musical taste preferences within Chinese hip‐hop and different modes of appreciation deployed by listeners. The findings reveal that young Chinese hip‐hop fans display cultural openness to foreign influences and genre mobility. The openness is employed as a marker of cultural capital for the Chinese middle class that values hybridity and innovation. However, even the most open‐minded individuals draw thematic hierarchies within hip‐hop through the unanimous rejection of vulgar and violent themes in favour of more serious and wholesome music. This rejection is framed as a strategy to authenticate hip‐hop within the broader popular culture arena while cultivating personal qualities of civility and wholesomeness that are highly valued in contemporary Chinese society. The paper contributes to the literature on musical taste preferences in cultural consumption by shedding light on the social relevance of cultural openness and music in contemporary Chinese popular culture.
Graph distance and feature‐guided multi‐view clustering: A novel method for clustering urban buildings
Authors: Yang Lin , Yang Ruolin , Zuo Zejun , Kwan Mei‐Po , Zhou Shunping 
Published: October 2023

AbstractUrban buildings are an integral component of urban space, and accurately identifying their spatial configurations and grouping them is vital for various urban applications. However, most existing building clustering methods only utilize the original spatial and nonspatial features of buildings, disregarding the potential value of complementary information from multiple perspectives. This limitation hinders their effectiveness in scenarios with intricate spatial configurations. To address this, this article proposes a novel multi‐view building clustering method that captures cross‐view information from spatial and nonspatial features. Drawing inspiration from both spatial proximity characteristics and nonspatial attributes, three views are established, including two spatial distance graphs (centroid distance graph and the nearest outlier distance graph) and a building attribute graph (multiple‐attribute graph). The three graphs undergo iterative cross‐diffusion processes to amplify similarities within each predefined graph view, culminating in their fusion into a unified graph. This fusion facilitates the comprehensive correlation and mutual enhancement of spatial and nonspatial information. Experiments were conducted using 10 real‐world community‐building datasets from Wuhan and Chengdu, China. The results demonstrate that our approach achieves 21.27% higher accuracy and 22.28% higher adjusted rand index in recognizing diverse complex arrangements compared to existing methods. These findings highlight the importance of leveraging complementary and consensus information across different feature dimensions for improving the performance of building clustering.
Phylogenetic diversity and functional potential of large and cell-associated viruses in the Bay of Bengal
Authors: Minch Benjamin , Akter Salma , Weinheimer Alaina , Rahman M. Shaminur , Parvez Md Anowar Khasru , Rezwana Rahman Sabita , Ahmed Md Firoz , Moniruzzaman Mohammad 
Published: October 2023

ABSTRACTThe Bay of Bengal (BoB) is the world’s largest bay, offering essential services like fishing and recreation while holding significant economic value for coastal communities. However, the BoB faces environmental challenges from monsoons, freshwater inputs, rising sea levels, and intensified cyclones due to climate change. Human activities such as tourism and development also impact the region, necessitating a global change perspective. Despite its importance, microbial diversity and ecology in the BoB remain largely unexplored. We focused on large and cell-associated viruses (i.e., originating from the cellular size fraction), particularly giant viruses and large phages in two BoB coastal sites: Cox’s Bazar, a populated beach with freshwater influences, and Saint Martin Island, a less affected resort island. Metagenomic sequencing reveals a higher abundance and diversity of viruses in Cox’s Bazar and presence of viruses that suggest freshwater intrusion and runoff. We identified 1962 putative phage genomes (10–655 kbp). Notably, 16 “large” phages >100 kbp were found in Saint Martin, and a terminase large subunit marker gene phylogeny revealed substantial diversity of large phages along the BoB coast. The BoB virome encodes diverse functionalities, with a greater presence of auxiliary metabolic genes in the Cox’s Bazar viral community. Additionally, five giant virus genomes (phylum Nucleocytoviricota) encoding various functionalities are reconstructed from Cox’s Bazar (83–876 kbp). This pioneering study revealing the viral diversity and host interactions in coastal BoB lays the foundation for future investigations into viral impact on biogeochemical cycles and the microbial food web in this understudied environment.IMPORTANCEThe BoB, the world’s largest bay, is of significant economic importance to surrounding countries, particularly Bangladesh, which heavily relies on its coastal resources. Concurrently, the BoB holds substantial ecological relevance due to the region’s high vulnerability to climate change-induced impacts. Yet, our understanding of the BoB’s microbiome in relation to marine food web and biogeochemical cycling remains limited. Particularly, there are little or no data on the viral diversity and host association in the BoB. We examined the viral community in two distinct BoB coastal regions to reveal a multitude of viral species interacting with a wide range of microbial hosts, some of which play key roles in coastal biogeochemical cycling or potential pathogens. Furthermore, we demonstrate that the BoB coast harbors a diverse community of large and giant viruses, underscoring the importance of investigating understudied environments to discover novel viral lineages with complex metabolic capacities.
Effect of high-risk versus low-risk pregnancy at the first antenatal care visit on the occurrence of complication during pregnancy and labour or delivery in Kenya: a double-robust estimation
Authors: Bagayoko Moussa , Kadengye Damazo T , Odero Henry Owoko , Izudi Jonathan 
Published: October 2023

ObjectivesWe evaluated the causal effects of high-risk versus low-risk pregnancy at the first antenatal care (ANC) visit on the occurrence of complications during pregnancy and labour or delivery among women in Kenya.MethodsWe designed a quasi-experimental study using observational data from a large mobile health wallet programme, with the exposure as pregnancy risk at the first ANC visit, measured on a binary scale (low vs high). Complications during pregnancy and at labour or delivery were the study outcomes on a binary scale (yes vs no). Causal effects of the exposure were examined using a double-robust estimation, reported as an OR with a 95% CI.ResultsWe studied 4419 women aged 10–49 years (mean, 25.6±6.27 years), with the majority aged 20–29 years (53.4%) and rural residents (87.4%). Of 3271 women with low-risk pregnancy at the first ANC visit, 833 (25.5%) had complications during pregnancy while 1074 (32.8%) had complications at labour/delivery. Conversely, of 1148 women with high-risk pregnancy at the first ANC visit, 343 (29.9%) had complication during pregnancy while 488 (42.5%) had complications at labour delivery. Multivariable adjusted analysis showed that women with high-risk pregnancy at the time of first ANC attendance had a higher occurrence of complications during pregnancy (adjusted OR (aOR) 1.22, 95% CI 1.02 to 1.46) and labour or delivery (aOR 1.20, 95% CI 1.03 to 1.41). In the double-robust estimation, a high-risk pregnancy at first ANC visit increased the occurrence of complications during pregnancy (OR 1.23, 95% CI 1.04 to 1.46) and labour or delivery (OR 1.24, 95% CI 1.07 to 1.45).ConclusionWomen with a high-risk pregnancy at the first ANC visit have an increased occurrence of complications during pregnancy and labour or delivery. These women should be identified early for close and appropriate obstetric and intrapartum monitoring and care to ensure maternal and neonatal survival.
Barbers’ compliance with biological hazard preventive measures and predictors in selected towns, Southeast Ethiopia: a cross-sectional study
Authors: Zenbaba Demisu , Sahiledengle Biniyam , Yassin Ahmed , Beressa Girma , Desta Fikreab , Engida Zinash Teferu , Nugusu Fikadu , Tufa Tilahun , Tekalegn Yohannes , Dibaba Diriba , Tasew Alelign , Wordofa Debebe , Assefa Tesfaye , Feleke Zegeye , Atlaw Daniel , Mesfin Telila , Geleta Eshetu Nigussie 
Published: October 2023

ObjectiveThe barber’s profession exposes their customers to several health risks. Negligence when using sharp instruments in barbershops can increase the risk of bloodborne infections, resulting in serious health problems for both the barber and the customer. Evidence for compliance with biological hazard preventive measures and predictors among barbers is critical and urgent. Thus, we aimed to assess compliance with biological hazard preventive measures and predictors among barbers in selected towns in Southeast Ethiopia.MethodsA cross-sectional study was conducted among 633 randomly selected barbers from 1 March to 30 March 2022. A multistage sampling followed by a systematic sampling and simple random sampling methods were used to select barbershops and barbers, respectively. We used a pretested interviewer-administered questionnaire and an observational checklist to collect data. Multinomial logistic regression analysis was used to identify predictors. An adjusted OR (AOR), along with a 95% CI and p<0.05, was used to estimate the strength of the association.ResultsThe good compliance level with biological hazard preventive measures was 47.10% (95% CI: 43.2%, 50.70%). Being married (AOR=3.04, 95% CI: 1.71, 8.56), aged from 25 to 34 years (AOR=2.75, 95% CI: 1.13, 8.06), served for ≤5 years (AOR=1.72, 95% CI: 1.24, 3.85), attending high school and above (AOR=4.32, 95% CI: 1.31, 12.73), being professional (AOR=6.34, 95% CI: 2.03, 11.36), having a positive attitude (AOR=2.95, 95% CI: 1.06, 8.22) and having good knowledge towards biological hazard preventive measures (AOR=3.14, 95% CI: 1.42, 9.25) were significant predictors of moderate and good compliance with biological hazard preventive measures.ConclusionThe study’s findings revealed that almost half of the barbers were in good compliance with biological hazard preventive measures. As a result, improving barbers’ attitudes and knowledge through on-the-job training is indispensable.
Systematic review and narrative synthesis of the key barriers and facilitators to the delivery and uptake of primary healthcare services to women in Pakistan
Authors: Rizvi Jafree Sara , Barlow Jane 
Published: October 2023

ObjectivesThe objective of this review is to (1) identify barriers and facilitators with respect to women’s health services at a primary care level based on a systematic review and narrative synthesis and (2) to conclude with recommendations for better services and uptake.DesignSystematic review and narrative synthesis.Data sourcesPubMed, BMC Medicine, Medline, CINAHL and the Cochrane Library. Grey literature was also searched.Eligibility criteriaQualitative, quantitative and mixed studies were included in the review.Data extraction and synthesisThe search took place at the beginning of June 2021 and was completed at the end of August 2021. Studies were included in the review based on the Sample, Phenomenon of Interest, Design, Evaluation, Research type criteria. The quality of the included studies was assessed using the Mixed Methods Appraisal Tool. Data were synthesised using a narrative synthesis approach.ResultsA total of 33 studies were included in the review. We identified six barriers to the delivery of effective primary healthcare for women’s health which have been organised under two core themes of ‘service barriers’ and ‘family/cultural barriers’. Ten barriers to the uptake of primary healthcare for women have been identified, under three core themes of ‘perceptions about healthcare service’, ‘cultural factors’ and ‘practical issues’. Three facilitators of primary healthcare delivery for women were identified: ‘motivating community health workers (CHWs) with continued training, salary, and supervision’ and ‘selection of CHWs on the basis of certain characteristics’. Five facilitators of the uptake of primary healthcare services for women were identified, under two core themes of ‘development of trust and acceptance’ and ‘use of technology’.ConclusionsChange is needed not only to address the limitations of the primary healthcare services themselves, but also the cultural practices and limited awareness and literacy that prevent the uptake of healthcare services by women, in addition to the wider infrastructure in terms of the provision of financial support, public transport and child care centres.PROSPERO registration numberCRD42020203472.
Comparison of SP263 and 22C3 immunohistochemistry PD-L1 assays for clinical efficacy of adjuvant atezolizumab in non-small cell lung cancer: results from the randomized phase III IMpower010 trial
Authors: Zhou Caicun , Srivastava Minu K , Xu Hao , Felip Enriqueta , Wakelee Heather , Altorki Nasser , Reck Martin , Liersch Rüdiger , Kryzhanivska Anna , Oizumi Satoshi , Tanaka Hiroshi , Hamm John , McCune Steven L , Bennett Elizabeth , Gitlitz Barbara , McNally Virginia , Ballinger Marcus , McCleland Mark , Zou Wei , Das Thakur Meghna , Novello Silvia 
Published: October 2023

BackgroundTumor samples from the phase III IMpower010 study were used to compare two programmed death-ligand 1 (PD-L1) immunohistochemistry assays (VENTANA SP263 and Dako 22C3) for identification of PD-L1 patient subgroups (negative, positive, low, and high expression) and their predictive value for adjuvant atezolizumab compared with best supportive care (BSC) in resectable early-stage non-small cell lung cancer (NSCLC).MethodsPD-L1 expression was assessed by the SP263 assay, which measured the percentage of tumor cells with any membranous PD-L1 staining, and the 22C3 assay, which scored the percentage of viable tumor cells showing partial or complete membranous PD-L1 staining.ResultsWhen examining the concordance at the PD-L1-positive threshold (SP263: tumor cell (TC)≥1%; 22C3: tumor proportion score (TPS)≥1%), the results were concordant between assays for 83% of the samples. Similarly, at the PD-L1–high cut-off (SP263: TC≥50%; 22C3: TPS≥50%), the results were concordant between assays for 92% of samples. The disease-free survival benefit of atezolizumab over BSC was comparable between assays for PD-L1-positive (TC≥1% by SP263: HR, 0.58 (95% CI: 0.40 to 0.85) vs TPS≥1% by 22C3: HR, 0.65 (95% CI: 0.45 to 0.95)) and PD-L1-high (TC≥50% by SP263: HR, 0.27 (95% CI: 0.14 to 0.53) vs TPS≥50% by 22C3: HR, 0.31 (95% CI: 0.16 to 0.60)) subgroups.ConclusionsThe SP263 and 22C3 assays showed high concordance and a comparable clinical predictive value of atezolizumab at validated PD-L1 thresholds, suggesting that both assays can identify patients with early-stage NSCLC most likely to experience benefit from adjuvant atezolizumab.Trial registration numberNCT02486718.
GTP Signaling Links Metabolism, DNA Repair, and Responses to Genotoxic Stress
Authors: Zhou Weihua , Zhao Zitong , Lin Angelica , Yang John Z. , Xu Jie , Wilder-Romans Kari , Yang Annabel , Li Jing , Solanki Sumeet , Speth Jennifer M. , Walker Natalie , Scott Andrew J. , Wang Lu , Wen Bo , Andren Anthony , Zhang Li , Kothari Ayesha U. , Yao Yangyang , Peterson Erik R. , Korimerla Navyateja , Werner Christian K. , Ullrich Alexander , Liang Jessica , Jacobson Janna , Palavalasa Sravya , O’Brien Alexandra M. , Elaimy Ameer L. , Ferris Sean P. , Zhao Shuang G. , Sarkaria Jann N. , Győrffy Balázs , Zhang Shuqun , Al-Holou Wajd N. , Umemura Yoshie , Morgan Meredith A. , Lawrence Theodore S. , Lyssiotis Costas A. , Peters-Golden Marc , Shah Yatrik M. , Wahl Daniel R. 
Published: October 2023

ABSTRACT

How cell metabolism regulates DNA repair is incompletely understood. Here, we define a GTP-mediated signaling cascade that links metabolism to DNA repair and has significant therapeutic implications. GTP, but not other nucleotides, regulates the activity of Rac1, a guanine nucleotide-binding protein, which promotes the dephosphorylation of serine 323 on Abl-interactor 1 (Abi-1) by protein phosphatase 5 (PP5). Dephosphorylated Abi-1, a protein previously not known to activate DNA repair, promotes nonhomologous end joining. In patients and mouse models of glioblastoma, Rac1 and dephosphorylated Abi-1 mediate DNA repair and resistance to standard-of-care genotoxic treatments. The GTP–Rac1–PP5–Abi-1 signaling axis is not limited to brain cancer, as GTP supplementation promotes DNA repair and Abi-1-S323 dephosphorylation in nonmalignant cells and protects mouse tissues from genotoxic insult. This unexpected ability of GTP to regulate DNA repair independently of deoxynucleotide pools has important implications for normal physiology and cancer treatment.

SIGNIFICANCE:
A newly described GTP-dependent signaling axis is an unexpected link between nucleotide metabolism and DNA repair. Disrupting this pathway can overcome cancer resistance to genotoxic therapy while augmenting it can mitigate genotoxic injury of normal tissues.
This article is featured in Selected Articles from This Issue, p. 5
Determinants for Antitumor and Protumor Effects of Programmed Cell Death
Authors: Workenhe Samuel T. , Inkol Jordon M. , Westerveld Michael J. , Verburg Shayla G. , Worfolk Sarah M. , Walsh Scott R. , Kallio Kaslyn L.F. 
Published: October 2023

Abstract
Cytotoxic anticancer therapies activate programmed cell death in the context of underlying stress and inflammatory signaling to elicit the emission of danger signals, cytokines, and chemokines. In a concerted manner, these immunomodulatory secretomes stimulate antigen presentation and T cell–mediated anticancer immune responses. In some instances, cell death–associated secretomes attract immunosuppressive cells to promote tumor progression. As it stands, cancer cell death–induced changes in the tumor microenvironment that contribute to antitumor or protumor effects remain largely unknown. This is complicated to examine because cell death is often subverted by tumors to circumvent natural, and therapy-induced, immunosurveillance. Here, we provide insights into important but understudied aspects of assessing the contribution of cell death to tumor elimination or cancer progression, including the role of tumor-associated genetics, epigenetics, and oncogenic factors in subverting immunogenic cell death. This perspective will also provide insights on how future studies may address the complex antitumor and protumor immunologic effects of cell death, while accounting for variations in tumor genetics and underlying microenvironment.