bluuebunny/crossref_metadata_2025_split huggingface dataset:
The excessive numbers of total nucleated cells does not affect the performance of the CliniMACS
Authors: Arpaci Fikret , Çetin Türker , Özet Ahmet , Oysul Kaan , Pekel Aysel , Ataergin Selmin , Kuzhan Okan , Kömürcü Şeref , Öztürk Bekir 
Published: December 2004

AbstractThis prospective study was carried out in healthy donors and patients. The performance of the CliniMACS was evaluated with the comparison of the numbers of total nucleated cell (TNC) within and over the capacity of the normal scale column. In addition, large vs. normal scale column and manual vs. automated washing systems were also compared. A total of 44 selections were done. Eighteen normal scale selections were done with initial TNC numbers over 6 × 1010 and 14 selections were performed below this number. None of the cases had CD34+ cell numbers over the capacity. Flow cytometry was used to check each separation performance for purity and recovery of CD34+ cells along with T‐ and B‐cell depletion level parameters. All healthy donors had significantly better mean purity and recovery of CD34+ cells, and T‐ and B‐cell depletion status than that of patients with values 95 vs. 85%, P: 0.006; 77 vs. 58%, P: 0.004; 4.55 log vs. 4.06 log, P: 0.004; 3.19 log vs. 2.63 log, P: 0.01, respectively. However, the performance of the system was not dependent on using the normal or large‐scale column; automated or manual washing systems; and initial TNC numbers above (>6 × 1010, range: 7.05–21.84 × 1010, mean: 12.32 × 1010 ) or within (<6 × 1010, range: 0.86–5.89 × 1010, mean: 4.15 × 1010) the column capacity. In conclusion, the performance of the CliniMACS is more efficient in healthy donors than in patients. However, the performance of the system did not change as long as the numbers of CD34+ cells (range: 0.34–5.87 × 108) were not exceeding the column capacity despite that more than 6 × 1010 TNCs were used. J. Clin. Apheresis 19:197–201, 2004. © 2004 Wiley‐Liss, Inc.
1,25‐dihydroxyvitamin D3 stimulated protein kinase C phosphorylation of type VI adenylyl cyclase inhibits parathyroid hormone signal transduction in rat osteoblastic UMR 106‐01 cells
Authors: Cheung Ricky , Erclik Mary S. , Mitchell Jane 
Published: December 2004

Abstract1,25‐Dihydroxyvitamin D3 (1,25(OH)2D3) treatment of osteoblastic cells was shown previously to attenuate Parathyroid hormone (PTH) response by inhibiting adenylyl cyclase (AC) activity. In this study, we have investigated the mechanism by which 1,25(OH)2D3 inhibits AC in rat osteoblastic UMR 106‐01 cells. 1,25(OH)2D3 treatment inhibited both PTH and forskolin‐stimulated AC activity by 25%–50% within 12 min in a concentration‐dependent manner suggesting a direct inhibition of the AC enzyme. Treatment with 25(OH)D3 had no effect on basal or stimulated AC activity. We determined the profile of AC subtypes expressed in UMR cells and found AC VI to be the dominant subtype accounting for 50% of AC mRNA. Since AC VI can be inhibited by protein kinase C (PKC) phosphorylation, we examined 1,25(OH)2D3 activation of various PKC isoforms. 1,25(OH)2D3 increased the membrane translocation of PKC‐βI, ‐δ, and ‐ζ with a concomitant increase in PKC activity. The translocation of PKC‐βI and ‐δ was blocked by the PLC inhibitor U73122 whereas that of PKC‐ζ was abolished by the PI‐3 kinase inhibitor wortmannin. The attenuation of cAMP production by 1,25(OH)2D3 was antagonized by the PKC inhibitors Gö6850, calphostin C, and wortmannin, but not by a calmodulin kinase II (CaMKII) inhibitor. Treatment with 1,25(OH)2D3 for 20 min increased AC VI phosphorylation by 10.8‐fold and this was blocked partially by Gö6850 and partially by wortmannin but was unaffected by CaMKII inhibitor. These results demonstrate that 1,25(OH)2D3 activation of PKC isoforms leads to phosphorylation of AC VI and inhibition of PTH‐activation of this pathway in osteoblasts. J. Cell. Biochem. 94: 1017–1027, 2005. © 2004 Wiley‐Liss, Inc.
Simulations of the active transport of a neutral solute based on a kinase‐channel‐phosphatase topology
Authors: Fiaty K. , Charcosset C. , Perrin B. , Couturier R. , Maïsterrena B. 
Published: December 2004

AbstractSimulations of coupled interactions involving two opposite enzymatic reactions, solute diffusions, and electrostatic interactions between membrane charges and charged solutes were conducted under a fixed kinase‐channel‐phosphatase (KCP) topology oriented from the outside to the inside of a porous membrane structure. Depending on the kinase and phosphatase locations, we recently demonstrated that an active transport of a phosphorylated substrate may occur via the opposite topology, that is, a PCK topology. The present analysis demonstrates that, under a KCP membrane topology, which also behaves as a specific ATP‐dependent transporter, the active transport of a neutral substrate may occur. This analogous active transport appears to be dependent on the phosphatase location and on the membrane surface potentials. A broad analysis of the role played by the main parameters taken into account in the model was conducted in order to define precisely the physico‐chemical conditions and the membrane topology needed for the highest active transports within the shortest time. © 2004 Wiley Periodicals, Inc. J Comput Chem 26: 201–213, 2005
Theoretical calculations of homoconjugation equilibrium constants in systems modeling acid–base interactions in side chains of biomolecules using the potential of mean force
Authors: Makowska Joanna , Makowski Mariusz , Liwo Adam , Chmurzyński Lech 
Published: December 2004

AbstractThe potentials of mean force (PMFs) were determined for systems forming cationic and anionic homocomplexes composed of acetic acid, phenol, isopropylamine, n‐butylamine, imidazole, and 4(5)‐methylimidazole, and their conjugated bases or acids, respectively, in three solvents with different polarity and hydrogen‐bonding propensity: acetonitrile (AN), dimethyl sulfoxide (DMSO), and water (H2O). For each pair and each solvent a series of umbrella‐sampling molecular dynamics simulations with the AMBER force field, explicit solvent, and counterions added to maintain a zero net charge of a system were carried out and the PMF was calculated by using the Weighted Histogram Analysis Method (WHAM). Subsequently, homoconjugation‐equilibrium constants were calculated by numerical integration of the respective PMF profiles. In all cases but imidazole stable homocomplexes were found to form in solution, which was manifested as the presence of contact minima corresponding to hydrogen‐bonded species in the PMF curves. The calculated homoconjugation constants were found to be greater for complexes with the OHO bridge (acetic acid and phenol) than with the NHN bridge and they were found to decrease with increasing polarity and hydrogen‐bonding propensity of the solvent (i.e., in the series AN > DMSO > H2O), both facts being in agreement with the available experimental data. It was also found that interactions with counterions are manifested as the broadening of the contact minimum or appearance of additional minima in the PMF profiles of the acetic acid‐acetate, phenol/phenolate system in acetonitrile, and the 4(5)‐methylimidazole/4(5)‐methylimidzole cation conjugated base system in dimethyl sulfoxide. © 2004 Wiley Periodicals, Inc. J Comput Chem 26: 235–242, 2005
Blocking sets of nonsecant lines to a conic in PG(2,q), q odd
Authors: Aguglia Angela , Korchmáros Gábor 
Published: December 2004

AbstractIn a previous paper 1, all point sets of minimum size in PG(2,q), blocking all external lines to a given irreducible conic ${\cal C}$, have been determined for every odd q. Here we obtain a similar classification for those point sets of minimum size, which meet every external and tangent line to ${\cal C}$. © 2004 Wiley Periodicals, Inc.
Constructing cyclic PBIBD(2)s through an optimization approach: Thirty‐two new cyclic designs
Authors: Morales Luis B. 
Published: December 2004

AbstractWe formulate the construction of cyclic partially balanced incomplete block designs with two associate classes (PBIBD(2)s) as a combinatorial optimization problem. We propose an algorithm based on tabu search to tackle the problem. Our algorithm constructed 32 new cyclic PBIBD(2)s. © 2004 Wiley Periodicals, Inc.
Influence of soil type and storage conditions on sensory qualities of fresh‐cut cantaloupe (Cucumis melo)
Authors: Bett‐Garber Karen L , Lamikanra Olusola , Lester Gene E , Ingram Daphne A , Watson Michael A 
Published: December 2004

AbstractOn‐farm cantaloupe (Cucumis melo) production as well as fresh‐cut storage duration can affect postharvest fruit sensory attributes. Both effects of soil type during production of cantaloupe fruits and storage temperature after fresh‐cut processing on sensory flavour and texture attributes were determined. Melons grown in sandy loam vs heavy clay soil were lower in sweet aromatic and sweet taste and higher in moisture release and fermented flavour. Fruity/melon, sweet aromatic, surface wetness, hardness and moisture release attributes decreased while fermented and sour flavour increased during storage regardless of soil type. During storage an increase in peroxidase activity occurred in fruits produced in sandy loam soil but decreased in fruits produced in clay soil. Clay soil appeared to have some advantages over sandy loam soil in producing cantaloupe fruits with better sensory quality attributes. Storage temperature conditions in this experiment (4 °C for 10 days or 4 °C for 4 days plus 10 °C for 6 days) did not have a statistically significant effect on these sensory attributes. Copyright © 2004 Society of Chemical Industry
Effect of various processing techniques and different levels of antioxidant on stability of rice bran during storage
Authors: Mujahid Ahmad , Ikram ul Haq  , Asif Musaddiq , Gilani Abrar H 
Published: December 2004

AbstractRice bran is a major cereal by‐product available for animal feeding in many parts of the world. It has a good balance of protein, fat, carbohydrates, fiber, vitamins and minerals. The greatest restriction to the use of rice bran is its instability during storage, leading to rancidity and the presence of heat‐labile antinutritional factors. Rice bran was treated to stabilize it by extrusion cooking, roasting, pelleting and adding antioxidant (125, 250 and 375 ppm). The rice bran so treated was stored for 345 days and analyzed every 15 days for free fatty acid, peroxide and iodine values. Heat treatments were effective in stabilizing rice bran by reducing the rancidity and increasing the storage life. Roasting was effective in stabilizing rice bran for only 180 days of storage. Raw and pelleted rice bran behaved similarly with regard to stability during storage for 345 days. Addition of antioxidant in rice bran was not effective for stabilizing FFA, peroxide and iodine values. There was no difference in dose rates of antioxidant of 125, 250 and 375 ppm. Extrusion cooking proved to be the most effective process for stabilizing rice bran for prolonged storage, and roasting would be the next choice. Addition of antioxidant in rice bran was not effective for stabilizing rice bran during storage. Copyright © 2004 Society of Chemical Industry
Effects of pressure on inclusion complexation of methylene blue with water‐soluble p‐sulfonatocalix[n]arenes
Authors: Sueishi Yoshimi , Inazumi Naoya , Hanaya Tadashi 
Published: December 2004

AbstractThe effects of pressure were examined for the inclusion complexation of water‐soluble p‐sulfonated calix[n]arenes (Calix‐Sn: n = 4, Calix‐S4; n = 6, Calix‐S6; n = 8, Calix‐S8) with methylene blue (MB). A differential pressure effect was found in the inclusion equilibria of Calix‐Sn. As the external pressure increases, the inclusion equilibrium of MB with Calix‐S4 and Calix‐S6 shifts to the dissociation side. However, with Calix‐S8, the inclusion equilibrium shifts to the association side with increased pressure. From the pressure dependence of the inclusion equilibria of Calix‐Sn, the reaction volumes, which are changes in volume accompanied by inclusion, were estimated as 32.1 cm3 mol−1 for Calix‐S4, 30.1 cm3 mol−1 for Calix‐S6 and −13.8 cm3 mol−1 for Calix‐S8. In addition, the structures of the inclusion complexes of Calix‐Sn with MB have been established by 1H NMR measurements. Based on the results, the behavior of inclusion complexation with Calix‐Sn is discussed. Copyright © 2004 John Wiley & Sons, Ltd.
Managing Eastern tent caterpillars Malacosoma americanum (F) on horse farms to reduce risk of mare reproductive loss syndrome
Authors: Potter Daniel A , Foss Leslie , Baumler Rebecca E , Held David W 
Published: December 2004

AbstractAn equine disease now known as mare reproductive loss syndrome (MRLS) struck the Ohio Valley in 2001–2002 causing thousands of foal abortions and enormous economic loss. Evidence that pregnant mares' exposure to Eastern tent caterpillars Malacosoma americanum (F) induces MRLS created an urgent call for control measures suitable for use on horse farms. We surveyed egg mass distribution and monitored emergence in wild cherry trees, and evaluated reduced‐risk treatment strategies including foliage sprays, trunk injections, winter egg mass treatments and barrier sprays to intercept larvae entering pastures. Egg masses were concentrated in the lower canopy, on exposed sides of trees. Larval emergence began in mid‐March. Emergence was prolonged (3–4 weeks) in 2002, a typically cool spring, but more synchronized in warmer 2003. Winter treatment of egg masses with bifenthrin or permethrin in a penetrating solvent prevented emergence, but 3% horticultural oil was ineffective for that purpose. Insecticidal soap or oil sprayed directly on neonates gave relatively poor control. Bifenthrin and spinosad were effective as foliage sprays against all instars, their field‐weathered residues active for at least 7 days. Bacillus thuringiensis Berliner var kurstaki controlled neonates within 3 days but was less active against late instars, with shorter residual action than bifenthrin or spinosad. Larvae did not avoid insecticide‐treated leaves. Trunk microinjection of cherry trees with dicrotophos was effective against all instars, whereas microinjection with milbemectin or abamectin gave poor or inconsistent control. Trunk injection with emamectin benzoate also was effective. Dry permethrin residues controlled late instars crawling in pasture grass for at least 7 days. Factors complicating M americanum management on horse farms are discussed. Copyright © 2004 Society of Chemical Industry
The antidiarrhoeal activity of Alchornea cordifolia leaf extract
Authors: Agbor Gabriel A. , Léopold Talla , Jeanne Ngogang Y. 
Published: December 2004

AbstractDiarrhoea is a public health problem in developing countries. It is therefore important and useful to identify plants with antidiarrhoeal activity. Alchornea cordifolia is quoted by many traditional healers as a plant with this activity. The antidiarrhoeal activity of its leaf extract was investigated against castor oil induced diarrhoea in mice, using morphine as the standard reference drug. A signiﬁcant (p < 0.01) dose related (100 mg/kg, 200 mg/kg, 400 mg/kg, 800 mg/kg) antidiarrhoeal activity of A. cordifolia leaf ethanol extract was observed with 800 mg/kg extract being the most effective. It delayed mouse intestinal transit accelerated by castor oil, inhibited the production of diarrhoeal faeces and modiﬁed the ﬂuid and electrolyte transport across the colonic mucosa when administered intraluminally. Phytochemical screening revealed the presence of tannins and ﬂavonoids which may account for the increased colonic water and electrolyte reabsorption, a mechanism suggested for the antidiarrhoeal activity of A. cordifolia. Copyright © 2004 John Wiley & Sons, Ltd.
Pharmacological effects of fermented red pepper
Authors: Choi Y. M. , Suh H. J. 
Published: December 2004

AbstractThe pharmacological effects were investigated of fermented red pepper (HF‐S), which consisted of 14.7% carbohydrate, 1.5% lipid, 4.9% protein, 0.3% ash, 78.2% moisture content, with 0.15% capsaicin and 0.06% dihydrocapsaicin. Oral administration of 0.25 mL HF‐S for 3 weeks produced signiﬁcant changes of the perirenal fat pad weight compared with the HF‐control group, suggesting a suppressive effect on lipid accumulation and a signiﬁcant decrease in the risk of arteriosclerosis. The HF‐S (0.25 mL) group also showed a lower plasma TG, TC level and atherogenic index than that of the HF‐control. In addition, the HF‐S (0.25 mL) group showed a marked increase in the production of glutathione, which is the major endogenous antioxidant, and a decrease in the production of lipid peroxide as the product of chemical damage by oxygen free radicals. It is assumed that the effect of HF‐S might relate to high glutathione production on the suppression of lipid peroxidation. HF‐S stimulated not only the proliferation of macrophages (as high as the positive control, LPS at 1000 µg/mL) but also mitogenic activity (1.2‐fold of LPS at 100 µg/mL). Copyright © 2004 John Wiley & Sons, Ltd.
Tannins and catechin gallate mediate the vasorelaxant effect of Arbutus unedo on the rat isolated aorta
Authors: Legssyer Abdelkhaleq , Ziyyat Abderrahim , Mekhﬁ Hassane , Bnouham Mohamed , Herrenknecht Christine , Roumy Vincent , Fourneau Christophe , Laurens Alain , Hoerter Jacqueline , Fischmeister Rodolphe 
Published: December 2004

AbstractThis study examined the vascular effect of Arbutus leaves (aqueous extract) and described the isolation of several fractions responsible for their vasorelaxant activity. The aqueous extract (AE) of leaves was tested on rat aortic rings precontracted with 0.1 µm noradrenaline. At 10−2 g/L, AE produced an endothelium dependent relaxation of 66% ± 5%, (n = 8). The leaves of Arbutus were then extracted successively with different solvents and the methanol extract was the most active. When tannins (primarily condensed tannins) were precipitated from the methanol extract, they showed a strong vasorelaxant activity (87% ± 4%, n = 5), whereas the elimination of tannins in the methanol extract reduced signiﬁcantly its vasorelaxant activity (42% ± 8%, n = 8, p < 0.005). The methanol extract was further separated semi‐preparatively by reversed‐phase HPLC. Four fractions (Fr2, Fr3, Fr4 and Fr6) were the most active and produced 88% ± 2% (n = 5), 75% ± 6% (n = 5), 76% ± 3% (n = 7) and 77% ± 3% (n = 10) relaxation, respectively. These four fractions mainly correspond to polyphenol compounds. Analysis of Fr6 indicated that this fraction contained catechin gallate. In conclusion, the vasorelaxant activity of Arbutus is likely to be due to polyphenol compounds, primarily condensed tannins and catechin gallate. Copyright © 2004 John Wiley & Sons, Ltd.
The effects of Pycnogenol on DNA damage in vitro and expression of superoxide dismutase and HP1 in Escherichia coli SOD and catalase deﬁcient mutant cells
Authors: Kim Young Gon , Park Hyeon Yong 
Published: December 2004

AbstractThe procyanidin‐rich French maritime pine bark extract Pycnogenol (PYC) is believed to be an antioxidant. To access whether PYC protects DNA against Fenton reaction radicals, pUC 19 plasmid DNA was damaged by OH− radicals generated from Fe(II) plus H2O2 in the presence and absence of PYC. DNA damage was quantiﬁed by measuring decreases in supercoiled DNA (SC‐DNA) using agarose gel electrophoresis. The results showed that PYC (50 µg/mL) did not inhibit DNA damage from Fenton reaction‐generated oxyradicals, when the Fenton reaction was allowed to proceed. However, PYC did protect against DNA damage when added prior to the initiation of the Fenton reaction, suggesting that protection resulted from chelation of Fe rather than from the scavenging of radicals. Moreover, we unexpectedly observed PYC‐associated DNA breakage, which was dose‐ and time‐ dependent. Other antioxidants such as desferrioxamine (DFO) including butylated hydroxyltoluene (BHT), curcumin and α‐tocopherol exhibited protective effects against DNA damage caused by the Fenton reaction. Subsequently, we assessed the possible pro‐oxidant function of PYC on DNA damage in E. coli SOD deﬁcient mutants under oxidative stress, after observing that PYC can induce SOD under oxidative stress. Although, PYC did not enhance DNA damage, it likewise did not protect against oxidative stress‐mediated DNA damage. All together, our data indicate that PYC under some circumstances can enhance oxidative stress‐mediated DNA damage in vitro and in vivo. Copyright © 2004 John Wiley & Sons, Ltd.
Effect of Momordica charantia on lipid proﬁle and oral glucose tolerance in diabetic rats
Authors: Chaturvedi P. , George S. , Milinganyo M. , Tripathi Y. B. 
Published: December 2004

AbstractIn this study, the methanol extract of Momordica charantia fruit extract was administered to diabetic rats to assess the long term effect of the extract on the lipid proﬁle and the oral glucose tolerance test. Treatment for 30 days showed a signiﬁcant decrease in triglyceride, low density lipoprotein and a signiﬁcant increase in high density lipoprotein level. A signiﬁcant effect on oral glucose tolerance was also noted. Chronic administration showed an improvement in the oral glucose tolerance curve. The effect was more pronounced when the test was done in rats fed the extract on the day of the test compared with tests done in rats which were not fed the extract on the same day. Copyright © 2004 John Wiley & Sons, Ltd.
The Influence of Sulfate‐Doping on the Nature of V Sites in VOx/TiO2 Catalysts
Authors: Brückner Angelika , Bentrup Ursula , Stelzer Jean‐Boris 
Published: December 2004

AbstractEPR, UV/Vis and FTIR spectroscopy as well as thermal analysis (TA/MS) were applied to study the influence of sulfate species present in the anatase support on the specific nature of VOx species in supported VOx/TiO2 catalysts. Those sulfate species modify the local structure of the supported vanadyl species and lead to the formation of two types of VO2+ sites instead of only one type being formed on sulfate‐free anatase. EPR and FTIR spectroscopic measurements revealed that a part of the VO2+ species are directly bound to the surface sulfate species. By TA/MS it was found that SO2 is released at lower temperature from VOx/TiO2 in comparison to the vanadium‐free support. The direct bonding between sulfate and VOx species stabilizes the latter on the surface of VOx/TiO2 resulting in three effects: 1) a higher V site dispersion in comparison to sulfate‐free TiO2, 2) a better resistance of surface vanadyls against diffusion into the bulk of the support and 3) a much faster reoxidation of reduced V sites than observed on sulfate‐free TiO2.
2, 4‐Dimethylpenta‐1, 3‐dien‐ und 2, 4‐Dimethylpentadienyl‐Komplexe des Rhodiums und Iridiums
Authors: Müller Jörn , Schiller Carsten , Escarpa Gaede Petra , Kempf Martin 
Published: December 2004

AbstractDie Komplexe [(η4‐C7H12)RhCl]2 (1) (C7H12 = 2, 4‐Dimethylpenta‐1, 3‐dien) und [(η4‐C7H12)2IrCl] (2) wurden durch Reaktion von [(η2‐C2H4)2RhCl]2 bzw. von [(η2‐Cycloocten)2IrCl]2 mit C7H12 erhalten. Die Umsetzung von 1 oder 2 mit CpTl (Cp = η5‐C5H5) liefert die Verbindungen [CpM(η4‐C7H12)] (3a: M = Rh; 3b: M = Ir). Die Hydridabstraktion am Pentadien‐Liganden von 3a, b mit Ph3CBF4 verläuft in Abhängigkeit vom Solvens unterschiedlich. In Aceton oder THF erhält man ausschließlich die “halboffenen” Metallocenium‐Komplexe [CpM(η5‐C7H11)]BF4 (4a: M = Rh; 4b: M = Ir). In Dichlormethan werden Gemische gebildet, die zusätzlich auch die durch elektrophile Substitution am Cp‐Ring entstandenen Spezies [(η5‐C7H11)M(η5‐C5H4CPh3)]BF4 (5a: M = Rh; 5b: M = Ir) sowie die η3‐2, 4‐Dimethylpentenyl‐Verbindung [(η3‐C7H13)Rh{η5‐C5H3(CPh3)2}]BF4 (6) enthalten. Durch Einwirkung von 2, 4‐Dimethylpentadienylkalium auf 1 bzw. 2 werden die Komplexe [(η4‐C7H12)M(η5‐C7H11)] (7a: M = Rh; 7b: M = Ir) gebildet, die in Lösung dynamisches Verhalten zeigen; Versuche zur Synthese der “offenen” Metallocenium‐Kationen [(η5‐C7H11)2M]+ durch Hydridabstraktion von 7a, b scheiterten jedoch. Die neuen Verbindungen wurden elementaranalytisch und spektroskopisch charakterisiert, 4b und 5a auch durch Röntgenstrukturanalyse.