bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prenylated isoflavonoids from Fabaceae against the NorA efflux pump in Staphylococcus aureus
Authors: Ika Irianti Marina , Vincken Jean-Paul , van Dinteren Sarah , ter Beest Ellen , Pos Klaas Martinus , Araya-Cloutier Carla 
Published: December 2023

AbstractOverexpression of NorA efflux pumps plays a pivotal role in the multidrug-resistance mechanism in S. aureus. Here, we investigated the activities of prenylated isoflavonoids, present in the legume plant family (Fabaceae), as natural efflux pump inhibitors (EPIs) in fluoroquinolone-resistant S. aureus. We found that four prenylated isoflavonoids, namely neobavaisoflavone, glabrene, glyceollin I, and glyceollin III, showed efflux pump inhibition in the norA overexpressing S. aureus. At sub-inhibitory concentrations, neobavaisoflavone (6.25 µg/mL, 19 µM) and glabrene (12.5 µg/mL, 39 µM), showed up to 6 times more Eth accumulation in norA overexpressing S. aureus than in the control. In addition, these two compounds boosted the MIC of fluoroquinolones up to eightfold. No fluoroquinolone potentiation was observed with these isoflavonoids in the norA knockout strain, indicating NorA as the main target of these potential EPIs. In comparison to the reported NorA EPI reserpine, neobavaisoflavone showed similar potentiation of fluoroquinolone activity at 10 µM, higher Eth accumulation, and less cytotoxicity. Neobavaisoflavone and glabrene did not exhibit membrane permeabilization effects or cytotoxicity on Caco-2 cells. In conclusion, our findings suggest that the prenylated isoflavonoids neobavaisoflavone and glabrene are promising phytochemicals that could be developed as antimicrobials and resistance-modifying agents to treat fluoroquinolone-resistant S. aureus strains.
Relationship between the jaw-closing force and dietary form in older adults without occlusal support requiring nursing care
Authors: Moritoyo Rieko , Nakagawa Kazuharu , Yoshimi Kanako , Yamaguchi Kohei , Ishii Miki , Yanagida Ryosuke , Namiki Chizuru , Tohara Haruka 
Published: December 2023

AbstractIn clinical practice, we encounter cases wherein older adults lacking occlusal support consume foods requiring mastication and adequate swallowing function. This study investigated the relationship between jaw-closing force (JCF) and dietary form in older adults without occlusal support requiring nursing care. This prospective cross-sectional study included 123 older adults requiring nursing care who lost their molar occlusal support and consumed food orally without dentures. JCF was defined as the force required for crushing food with the edentulous ridges or with the tooth and edentulous ridge while closing the mouth. Participants were classified into four groups based on the International Dysphagia Diet Standardization Initiative framework for recommended dietary forms. Basic information was collected, and tongue pressure and JCF were measured. Differences in JCF were analyzed using one-way analysis of variance, while factors related to dietary form were evaluated using ordinal logistic regression analysis. Significant differences in JCF were observed among the four groups. Factors such as the Barthel Index, tongue pressure, and JCF were dietary form-related. Our findings suggest that older adults requiring nursing care tend to have higher JCF when consuming meals requiring mastication. Therefore, JCF could serve as an index for determining appropriate dietary forms in this population.
Feasibility and safety of choledochotomy primary closure in laparoscopic common bile duct exploration without biliary drainage: a retrospective study
Authors: Lai Wei , Xu Nan 
Published: December 2023

AbstractCommon bile duct (CBD) exploration and T-tube drainage are the main surgical methods for the removal of bile duct stones (BDSs), which can now be completed by laparoscopy. However, the feasibility and safety of primary closure of the CBD (PCCBD) in laparoscopic CBD exploration (LCBDE) without biliary drainage are still uncertain. From January 1, 2021, to June 30, 2022, patients who were diagnosed with BDSs and underwent LCBDE and primary closure of the CBD without biliary drainage in our hospital were included. The clinical and prognostic data of the patients were retrospectively analyzed to determine the feasibility and safety of PCCBD in LCBDE without biliary drainage. Forty-nine patients successfully underwent PCCBD in LCBDE without biliary drainage. The operation time was 158.8 ± 50.3 (90–315,150) minutes, the bile duct suture time was 17.6 ± 4.46 (10–26, 18) minutes, the intraoperative blood loss volume was 70.4 ± 52.6 (5–200, 80) ml, the hospitalization cost was 28,141.2 ± 7011.3 (15,005.45–52,959.34, 26,815.14) CNY Yuan, the hospitalization time was 13.22 ± 5.16 (8–32, 12) days, and the postoperative hospitalization time was 7.31 ± 1.94 (3–15, 7) days. There were 3 cases of postoperative bile leakage (3/49, 6.12%), all of them healed by nonsurgical treatment. During the follow-up of 17.2 ± 11.01 (10–26, 17) months, no residual BDSs, biliary stricture or other complications classified as Clavien-Dindo grade I or higher occurred. For some selected patients who meet certain criteria, PCCBD in LCBDE without biliary drainage is feasible and safe and is more conducive to the rapid postoperative recovery of patients.
A tale of two parasites: Responses of honey bees infected with Nosema ceranae and Lotmaria passim
Authors: MacInnis Courtney I. , Luong Lien T. , Pernal Stephen F. 
Published: December 2023

AbstractNosema ceranae and Lotmaria passim are two commonly encountered digestive tract parasites of the honey bee that have been associated with colony losses in Canada, the United States, and Europe. Though honey bees can be co-infected with these parasites, we still lack basic information regarding how they impact bee health at the individual and colony level. Using locally-isolated parasite strains, we investigated the effect of single and co-infections of these parasites on individual honey bee survival, and their responsiveness to sucrose. Results showed that a single N. ceranae infection is more virulent than both single L. passim infections and co-infections. Honey bees singly infected with N. ceranae reached < 50% survival eight days earlier than those inoculated with L. passim alone, and four days earlier than those inoculated with both parasites. Honey bees infected with either one, or both, parasites had increased responsiveness to sucrose compared to uninfected bees, which could correspond to higher levels of hunger and increased energetic stress. Together, these findings suggest that N. ceranae and L. passim pose threats to bee health, and that the beekeeping industry should monitor for both parasites in an effort correlate pathogen status with changes in colony-level productivity and survival.
Two-dimensional Brownian motion of active particle on superfluid helium surface
Authors: Boltnev Roman E. , Vasiliev Mikhail M. , Petrov Oleg F. 
Published: December 2023

AbstractWe report an experimental study of the 2D dynamics of active particles driven by quantum vortices on the free surface of superfluid helium at T = 1.45 К. The particle motion at short times (< 25 ms) relates to anomalous diffusion mode typical for active particles, while for longer times it corresponds to normal diffusion mode. The values of the rotational and translational kinetic energies of the particle allow to determine for the first time the intensity of the particle-vortex interaction and the dissipation rate of the vortex bundle energy. Strong bonding between a particle and a vortex is explained by coupling of normal and superfluid components.
A closer look at the time course of bodily responses to awe experiences
Authors: Takano Ryota , Nomura Michio 
Published: December 2023

AbstractThe structure and function of awe have been examined by focusing on the average level of outcomes during awe experiences. In the present study, we tested the psychophysiological process of experiencing awe, focusing on time-series changes in skin conductance responses (SCRs), a moment-by-moment measure of sympathetic nervous responses, and pupil diameter, which is dilated or constricted through the sympathetic and parasympathetic nervous system. The responses were measured through an experiment where 77 Japanese university students watched emotional (awe, amusement, or neutral) videos while moving a joystick when they felt supernatural agency or non-agency, examining the underlying psychological processes. We found that experiencing awe was associated with frequent and steep changes in SCRs and frequent changes in pupil diameter. The joystick inclination, the perceptions of the supernatural, was kept at a high level from the start to the end of awe experiences. These results may reflect the psychophysiological processes of awe: the “fluctuation” of the sympathetic nervous system might underlie awe-specific experiences. Our findings shed new light on the mechanisms of the body-mind interaction in awe experiences.
Investigation of individual strategies in the aerial phase in ski jumping
Authors: Jølstad Petter Andre Husevåg , Gilgien Matthias , Elfmark Ola 
Published: December 2023

AbstractThe purpose of this investigation was to examine the performance strategy of three ski jumpers during the steady glide phase and explain how different strategical solutions can lead to jumps of roughly the same length. In this study, a total of 24 jumps performed by two World Cup (WC) athletes and one Continental Cup (COC) athlete were measured with a differential Global Navigation Satellite System (dGNSS) on a large ski jumping hill. For each athlete, the continuous position data, velocity, aerodynamic forces and lift-to-drag ratio (LD-ratio) were averaged and compared for the steady glide phase to examine individual jump strategies. The dGNSS yielded accurate measurements of trajectory, velocity and aerodynamic forces, revealing clear differences between the athletes. The largest differences were found between the WC athletes and the COC athlete. The WC athletes focused on maximizing horizontal velocity while the COC athlete minimized vertical velocity. This difference may be explained by the different hill sizes the athletes normally compete on. One of the WC athletes consistently increased their horizontal velocity during the end of the steady glide phase by maintaining a high LD-ratio, which highlights the effect of aerodynamics on the resulting velocity, trajectory and jump length.
Observation of superconductivity and enhanced upper critical field of η-carbide-type oxide Zr4Pd2O
Authors: Watanabe Yuto , Miura Akira , Moriyoshi Chikako , Yamashita Aichi , Mizuguchi Yoshikazu 
Published: December 2023

AbstractWe report the first observation of bulk superconductivity of a η-carbide-type oxide Zr4Pd2O. The crystal structure and the superconducting properties were studied through synchrotron X-ray diffraction, magnetization, electrical resistivity, and specific heat measurement. The superconducting transition was observed at Tc = 2.73 K. Our measurement revealed that the η-carbide-type oxide superconductor Zr4Pd2O shows an enhanced upper critical field μ0Hc2(0) = 6.72 T, which violates the Pauli-Clogston limit μ0HP = 5.29 T. On the other hand, we found that the enhanced upper critical field is absent in a Rh analogue Zr4Rh2O. The large μ0Hc2(0) of Zr4Pd2O would be raised from strong spin–orbit coupling with Pd-4d electrons. The discovery of new superconducting properties for Zr4Pd2O would shed light on the further development of η-carbide-type oxide superconductors.
Improved exponential type ratio estimator in double sampling for stratification
Authors: Gupta Anurag , Tailor Rajesh , Barod Nitu 
Published: December 2023

AbstractThe objective of this research is to create a chain-ratio-type exponential estimator in order to estimate the finite population mean in double sampling for stratification. An estimator for population mean has been constructed based on the concept of chain-ratio estimators. The constructed estimator is compared to the standard unbiased estimator, as well as the other relevant existing estimators and conditions are shown to yield better results in terms of efficiency. To support the theoretical results the study has been done on both natural as well as simulated populations.
Renoprotective effects of extracellular fibroblast specific protein 1 via nuclear factor erythroid 2-related factor-mediated antioxidant activity
Authors: Takahashi Naoki , Yokoi Seiji , Kimura Hideki , Naiki Hironobu , Matsusaka Taiji , Yamamoto Yasuhiko , Nakatani Kimihiko , Kasuno Kenji , Iwano Masayuki 
Published: December 2023

AbstractPodocyte expression of fibroblast specific protein 1 (FSP1) is observed in various types of human glomerulonephritis. Considering that FSP1 is secreted extracellularly and has been shown to have multiple biological effects on distant cells, we postulated that secreted FSP1 from podocytes might impact renal tubules. Our RNA microarray analysis in a tubular epithelial cell line (mProx) revealed that FSP1 induced the expression of heme oxygenase 1, sequestosome 1, solute carrier family 7, member 11, and cystathionine gamma-lyase, all of which are associated with nuclear factor erythroid 2-related factor (Nrf2) activation. Therefore, FSP1 is likely to exert cytoprotective effects through Nrf2-induced antioxidant activity. Moreover, in mProx, FSP1 facilitated Nrf2 translocation to the nucleus, increased levels of reduced glutathione, inhibited the production of reactive oxygen species (ROS), and reduced cisplatin-induced cell death. FSP1 also ameliorated acute tubular injury in mice with cisplatin nephrotoxicity, which is a representative model of ROS-mediated tissue injury. Similarly, in transgenic mice that express FSP1 specifically in podocytes, tubular injury associated with cisplatin nephrotoxicity was also mitigated. Extracellular FSP1 secreted from podocytes acts on downstream tubular cells, exerting renoprotective effects through Nrf2-mediated antioxidant activity. Consequently, podocytes and tubular epithelial cells have a remote communication network to limit injury.
Randomized phase II study of preoperative afatinib in untreated head and neck cancers: predictive and pharmacodynamic biomarkers of activity
Authors: Marret Grégoire , Temam Stéphane , Kamal Maud , Even Caroline , Delord Jean-Pierre , Hoffmann Caroline , Dolivet Gilles , Malard Olivier , Fayette Jérôme , Capitain Olivier , Vergez Sébastien , Geoffrois Lionel , Rolland Frédéric , Zrounba Philippe , Laccourreye Laurent , Saada-Bouzid Esma , Aide Nicolas , Bénavent Valérie , Klijianenko Jerzy , Lamy Constance , Girard Elodie , Vacher Sophie , Masliah-Planchon Julien , de Koning Leanne , Puard Vincent , Borcoman Edith , Jimenez Marta , Bièche Ivan , Gal Jocelyn , Le Tourneau Christophe 
Published: December 2023

AbstractThere is no strong and reliable predictive biomarker in head and neck squamous cell carcinoma (HNSCC) for EGFR inhibitors. We aimed to identify predictive and pharmacodynamic biomarkers of efficacy of afatinib, a pan-HER tyrosine kinase inhibitor, in a window-of-opportunity trial (NCT01415674). Multi-omics analyses were carried out on pre-treatment biopsy and surgical specimen for biological assessment of afatinib activity. Sixty-one treatment-naïve and operable HNSCC patients were randomised to afatinib 40 mg/day for 21–28 days versus no treatment. Afatinib produced a high rate of metabolic response. Responders had a higher expression of pERK1/2 (P = 0.02) and lower expressions of pHER4 (P = 0.03) and pRB1 (P = 0.002) in pre-treatment biopsy compared to non-responders. At the cellular level, responders displayed an enrichment of tumor-infiltrating B cells under afatinib (P = 0.02). At the molecular level, NF-kappa B signaling was over-represented among upregulated genes in non-responders (P < 0.001; FDR = 0.01). Although exploratory, phosphoproteomics-based biomarkers deserve further investigations as predictors of afatinib efficacy.
Validity of heart rate derived core temperature estimation during simulated firefighting tasks
Authors: Agostinelli Philip J. , Linder Braxton A. , Frick Katherine A. , Bordonie Nicholas C. , Neal Frances K. , Sefton JoEllen M. 
Published: December 2023

AbstractRectal core temperature monitoring can help fire services mitigate heat injury but can be invasive and impractical. EQ02 + LifeMonitor provides a non-invasive estimation of core temperature. Therefore, the primary purpose of this study was to determine the validity of the EQ02 + LifeMonitor compared to the gold standard rectal thermometer core temperature assessment, as well as the potential influence of turnout gear on the estimated and physiological strain experienced during these activities. Thirteen participants completed simulated firefighting tasks with and without turnout gear, involving four rounds of a 5-min walk on a treadmill at 2.8 mph/2.5% grade and 20 deadlifts over 5 min in an environmental chamber set to 40.6 °C; 50% humidity. During each trial participants wore both an EQ02 + LifeMonitor and DataTherm II rectal thermometer. The results from the devices were statistically equivalent (p < 0.001), yet there was a statistically significant difference in the value (~ 0.1 °C; p < 0.001). There was a significant effect of devices [p < 0.001] and time [p < 0.001], but no interaction effect [p = 0.70] on core temperature drift. Estimated core temperature was marginally different from that measured via the DataTherm II. The EQ02 on average overestimated core temperature. Heart rate, rating of perceived exertion, and area under the curve of core temperature were significantly elevated due to turnout gear [ps < 0.025], but not core temperature skin temperature, or ventilatory rate [ps > 0.372]. These results suggest the EQ02 + LifeMonitor may be a viable, non-invasive alternative for assessing core temperature compared to rectal temperature monitoring, especially during rigorous, intermittent activities. Turnout gear does however increase heart rate, cumulative core temperature, and perceived exertion. Additionally, the validity of the estimated core temperature is not impacted by the use turnout gear. This is likely due to significant changes in heart rate, which allowed the heart-rate derived estimate of core temperature to remain consistent with changes in DataTherm II rectal temperatures.
Hemodynamic effects of entry and exit tear size in aortic dissection evaluated with in vitro magnetic resonance imaging and fluid–structure interaction simulation
Authors: Zimmermann Judith , Bäumler Kathrin , Loecher Michael , Cork Tyler E. , Marsden Alison L. , Ennis Daniel B. , Fleischmann Dominik 
Published: December 2023

AbstractUnderstanding the complex interplay between morphologic and hemodynamic features in aortic dissection is critical for risk stratification and for the development of individualized therapy. This work evaluates the effects of entry and exit tear size on the hemodynamics in type B aortic dissection by comparing fluid–structure interaction (FSI) simulations with in vitro 4D-flow magnetic resonance imaging (MRI). A baseline patient-specific 3D-printed model and two variants with modified tear size (smaller entry tear, smaller exit tear) were embedded into a flow- and pressure-controlled setup to perform MRI as well as 12-point catheter-based pressure measurements. The same models defined the wall and fluid domains for FSI simulations, for which boundary conditions were matched with measured data. Results showed exceptionally well matched complex flow patterns between 4D-flow MRI and FSI simulations. Compared to the baseline model, false lumen flow volume decreased with either a smaller entry tear (− 17.8 and − 18.5%, for FSI simulation and 4D-flow MRI, respectively) or smaller exit tear (− 16.0 and − 17.3%). True to false lumen pressure difference (initially 11.0 and 7.9 mmHg, for FSI simulation and catheter-based pressure measurements, respectively) increased with a smaller entry tear (28.9 and 14.6 mmHg), and became negative with a smaller exit tear (− 20.6 and − 13.2 mmHg). This work establishes quantitative and qualitative effects of entry or exit tear size on hemodynamics in aortic dissection, with particularly notable impact observed on FL pressurization. FSI simulations demonstrate acceptable qualitative and quantitative agreement with flow imaging, supporting its deployment in clinical studies.
Col1α2-Cre-mediated recombination occurs in various cell types due to Cre expression in epiblasts
Authors: Matsumoto Yuzuru , Ikeda Shinya , Kimura Takeshi , Ono Koh , Ashida Noboru 
Published: December 2023

AbstractThe Cre-LoxP system has been commonly used for cell-specific genetic manipulation. However, many Cre strains exhibit excision activity in unexpected cell types or tissues. Therefore, it is important to identify the cell types in which recombination takes place. Fibroblasts are a cell type that is inadequately defined due to a lack of specific markers to detect the entire cell lineage. Here, we investigated the Cre recombination induced by Col1α2-iCre, one of the most common fibroblast-mesenchymal Cre driver lines, by using a double-fluorescent Cre reporter line in which GFP is expressed when recombination occurs. Our results indicated that Col1α2-iCre activity was more extensive across cell types than previously reported: Col1α2-iCre-mediated recombination was found in not only cells of mesenchymal origin but also those of other lineages, including haematopoietic cells, myocardial cells, lung and intestinal epithelial cells, and neural cells. In addition, study of embryos revealed that recombination by Col1α2-iCre was observed in the early developmental stage before gastrulation in epiblasts, which would account for the recombination across various cell types in adult mice. These results offer more insights into the activity of Col1α2-iCre and suggest that experimental results obtained using Col1α2-iCre should be carefully interpreted.