bluuebunny/crossref_metadata_2025_split huggingface dataset:
An LPS-dephosphorylating alkaline phosphatase of PhoA family from the marine bacteriumCobetia amphilectiKMM 296 and multiplicity of alkaline phosphatase families inCobetiaspp
Authors: Balabanova Larissa , Bakholdina Svetlana , Buinovskaya Nina , Noskova Yulia , Kolpakova Oksana , Vlasova Vanessa , Bondarev Georgii , Seitkalieva Aleksandra , Son Oksana , Tekutyeva Liudmila 
Published: January 2024

AbstractA highly active alkaline phosphatase (ALP) from the mollusk strain of the marine bacteriumCobetia amphilectiKMM 296 (CmAP) was found to remove phosphorus from theEscherichia colilipopolysaccharides (LPS). Phylogenetic analysis of the amino acid sequences of ALPs found in 36 availableCobetiagenomes revealed that CmAP and its homologues from nine strains clustered together with the human and squid LPS-detoxifying enzymes. Each strain of the genusCobetiahas a variety of ALPs mostly of the PhoD and PhoX families. The PhoA gene encoding for the CmAP-like ALP is characteristic for the subspeciesC. amphilecti, with a complete set of four ALP families, including PafA and two PhoD structures (5 genes). However, a single strain of the speciesCobetia crustatorumJO1Tfrom fermented shrimp, phylogenetically distant fromC. amphilectiandC. marina, among four ALPs contains a CmAP homologue carrying an inactive mutation. Apparently, the multiplicity of ALPs in bacteria of the genusCobetiais a trait of incredible adaptation to a phosphorus-depleted environment and a specialty of organophosphate destructor in eco-niches to which they once emerged, includingZosteraspp. roots. The ALP clusterization and an identity level of the genus-specific biosynthetic genes encoding for ectoine and polyketide cluster T1PKS, responsible for sulfated extracellular polysaccharide synthesis, coincide with a new whole genome-based taxonomic classification of the genusCobetia. The LPS-dephosphorylating property of the PhoA familyC. amphilectiALP CmAP may be used in the development of anti-inflammatory drugs.
Metagenomic Insights Reveal Unrecognized Diversity of Entotheonella in JapaneseTheonellaSponges
Authors: Yamabe Sota , Yoshitake Kazutoshi , Ninomiya Akihiro , Piel Jörn , Takeyama Haruko , Matsunaga Shigeki , Takada Kentaro 
Published: January 2024

AbstractNumerous biologically active natural products have been discovered from marine sponges, particularly fromTheonella swinhoei, which is known to be a prolific source of natural products such as polyketides and peptides. Recent studies have revealed that many of these natural products are biosynthesized byCandidatusEntotheonella phylotypes, which are uncultivated symbionts withinT. swinhoei. Consequently, Entotheonella is considered an untapped biochemical resource. In this study, we conducted metagenomic analyses to assess the diversity of Entotheonella in fourT. swinhoeiindividuals (two each of chemotypes Y and W), after separating filamentous bacteria using density gradient centrifugation. We obtained five Entotheonella genomes from filamentous bacteria-enriched fractions. Notably, one of these genomes is significantly different from previously reported Entotheonella variants. Additionally, we identified closely related Entotheonella members present across different chemotypes ofT. swinhoei. Thus, our metagenomic insights reveal that the diversity of Entotheonella withinTheonellasponges is greater than previously recognized.
High-throughput single-cell, single-mitochondrial DNA assay using hydrogel droplet microfluidics
Authors: Park Juhwan , Kadam Parnika S. , Atiyas Yasemin , Chhay Bonirath , Tsourkas Andrew , Eberwine James H. , Issadore David A. 
Published: January 2024

AbstractThere is growing interest in understanding the biological implications of single cell heterogeneity and intracellular heteroplasmy of mtDNA, but current methodologies for single-cell mtDNA analysis limit the scale of analysis to small cell populations. Although droplet microfluidics have increased the throughput of single-cell genomic, RNA, and protein analysis, their application to sub-cellular organelle analysis has remained a largely unsolved challenge. Here, we introduce an agarose-based droplet microfluidic approach for single-cell, single-mtDNA analysis, which allows simultaneous processing of hundreds of individual mtDNA molecules within >10,000 individual cells. Our microfluidic chip encapsulates individual cells in agarose beads, designed to have a sufficiently dense hydrogel network to retain mtDNA after lysis and provide a robust scaffold for subsequent multi-step processing and analysis. To mitigate the impact of the high viscosity of agarose required for mtDNA retention on the throughput of microfluidics, we developed a parallelized device, successfully achieving ~95% mtDNA retention from single cells within our microbeads at >700,000 drops/minute. To demonstrate utility, we analyzed specific regions of the single mtDNA using a multiplexed rolling circle amplification (RCA) assay. We demonstrated compatibility with both microscopy, for digital counting of individual RCA products, and flow cytometry for higher throughput analysis.
Convergence for Inactivation of TGFβ Signaling Is a Common Feature of Advanced Pancreatic Cancer
Authors: Hong Jungeui , Kohutek Zachary , Zhang Haochen , Karnoub Elias-Ramzey , Kappagantula Rajya , Wood Laura D. , Iacobuzio-Donahue Christine 
Published: January 2024

AbstractWe performed WES of 250 unique tumor tissues from 30 multiregion sampled pancreatic cancer research autopsies from patients diagnosed with advanced stage disease. We find that most genetic alterations in PDAC occur in a subclonal manner, and some genes occurred in a subclonal manner exclusively. Convergent evolution within the TGFβ pathway was also identified as a common feature of advanced stage disease, withSMAD4inactivation more common among metastatic PDACs compared to inactivation of TGFβ surface receptors that was more common in locally advanced tumors. The mode of clinical management (radiation versus chemotherapy) contributed distinct mutational signatures yet these mutations are not predicted to have functional relevance to tumor progression. Overall, these findings provide a first definition of the genetic features that distinguish among patients with locally advanced versus metastatic PDAC. These findings may have clinical relevance in upfront clinical decision making for the optimal candidates for neoadjuvant therapy.
Urolithin A improves Alzheimer’s disease cognition and restores mitophagy and lysosomal functions
Authors: Hou Yujun , Chu Xixia , Park Jae-Hyeon , Zhu Qing , Hussain Mansoor , Li Zhiquan , Madsen Helena Borland , Yang Beimeng , Wei Yong , Wang Yue , Fang Evandro F. , Croteau Deborah L. , Bohr Vilhelm A. 
Published: January 2024

ABSTRACTBackgroundCompromised autophagy, including impaired mitophagy and lysosomal function, is thought to play a pivotal role in Alzheimer’s disease (AD). Urolithin A (UA) is a gut microbial metabolite of ellagic acid that stimulates mitophagy. The effects of early and/or long-term treatment, as well as more detailed mechanisms of action, are not known.MethodsWe addressed these questions in three mouse models of AD, and behavioral, electrophysiological and biochemistry assays were performed.ResultsLong-term UA treatment significantly improved learning, memory and olfactory function in different AD transgenic mice. UA also reduced Aβ and Tau pathologies, and improved long-term potentiation. We found that UA activated autophagy/mitophagy via increasing lysosomal functions. At the cellular level, UA improved lysosomal function and normalized lysosomal cathepsins, especially targeting cathepsin Z, to restore lysosomal function in AD, indicating the important role of cathepsins in UA-induced therapeutic effects of AD.ConclusionsCollectively, our study highlights the importance of lysosomal dysfunction in AD etiology, and points to the high translational potential of UA.
Intratumoral Microbiome of Adenoid Cystic Carcinomas and Comparison with other Head and Neck Cancers
Authors: Karpinets Tatiana V. , Mitani Yoshitsugu , Chang Chia-Chi , Wu Xiaogang , Song Xingzhi , Flores Ivonne I , McDaniel Lauren K , Hoballah Yasmine M , Veguilla Fabiana J , Ferrarotto Renata , Colbert Lauren E , Ajami Nadim J , Jenq Robert R , Zhang Jianhua , Futreal Andrew P , El-Naggar Adel K. 
Published: January 2024

ABSTRACTBackgroundAdenoid cystic carcinoma (ACC) is a rare, slow growing yet aggressive head and neck malignancy. Despite its clinical significance, our understanding of the cellular evolution and microenvironment in ACC remains limited.MethodsWe investigated the intratumoral microbiome of 50 ACC tumors and 33 adjacent normal tissues using 16S rRNA gene sequencing. This allowed us to characterize the bacterial communities within ACC and explore potential associations between the bacterial community structure, patient’s clinical characteristics, and tumor molecular features obtained through RNA sequencing.ResultsBacterial composition in ACC displayed significant differences compared to adjacent normal salivary tissue and exhibited diverse levels of species richness. We identified two main microbial subtypes within ACC: oral-like and gut-like. Oral-like microbiomes, characterized by higher diversity and abundance of genera likeNeisseria, Leptotrichia, Actinomyces, Streptococcus, Rothia, andVeillonella(commonly found in healthy oral cavities), were associated with the less aggressive ACC-II molecular subtype and improved patient outcomes. Notably, we identified the same oral genera in oral cancer and in head and neck squamous cell carcinomas. In both cancers, they were part of shared oral communities associated with more diverse microbiome, less aggressive tumor phenotype, and better survival. Conversely, gut-like microbiomes in ACC, featuring low diversity and colonization by gut mucus layer-degrading species likeBacteroides, Akkermansia, Blautia, Bifidobacterium, andEnterococcus, were associated with poorer outcomes. Elevated levels ofBacteroides thetaiotaomicronwere independently associated with significantly worse survival, regardless of other clinical and molecular factors. Furthermore, this association positively correlated with tumor cell biosynthesis of glycan-based cell membrane components.ConclusionsOur study uncovers specific intratumoral oral genera as potential pan-cancer biomarkers for favorable microbiomes in ACC and other head and neck cancers. These findings highlight the pivotal role of the intratumoral microbiome in influencing ACC prognosis and disease biology.
Heavy Higgs boson searches at the LHC in light of a left-right symmetric model
Authors: Bhattacharyya Sanchari 
Published: January 2024

We investigate a left-right symmetric model respecting SU(3)C⊗SU(2)L⊗U(1)L⊗SU(2)R⊗U(1)R local gauge symmetry. We study the interactions of the heavy neutral and charged scalars of this model along with their production at the hadron collider and their subsequent decays. We analyze the collider searches of two heavy scalars, one of them is charge neutral and another one is singly charged. In both the cases we consider their associated production at the Large Hadron Collider (LHC) and finally concentrate only on the leptonic final states. We perform both cut-based and multivariate analysis using the boosted decision tree algorithm for 14 TeV as well as 27 TeV LHC run with 3000  fb−1 integrated luminosity. As expected, the multivariate analysis shows a better signal-background discrimination compared to the cut-based analysis. In this article, we show that a charged Higgs of mass 750 GeV and 1.2 TeV can be probed with 2.77σ (4.58σ) and 1.38σ (3.66σ) significance at 14 (27) TeV run of LHC.

Published by the American Physical Society
2024
Entanglement Phase Transition Due to Reciprocity Breaking without Measurement or Postselection
Authors: Lee Gideon , Jin Tony , Wang Yu-Xin , McDonald Alexander , Clerk Aashish 
Published: January 2024

Despite its fully unitary dynamics, the bosonic Kitaev chain (BKC) displays key hallmarks of non-Hermitian physics, including nonreciprocal transport and the non-Hermitian skin effect. Here, we demonstrate another remarkable phenomena: the existence of an entanglement phase transition (EPT) in a variant of the BKC that occurs as a function of a Hamiltonian parameter g and which coincides with a transition from a reciprocal to a nonreciprocal phase. As g is reduced below a critical value, the postquench entanglement entropy of a subsystem of size l goes from a volume-law phase, where it scales as l, to a -law phase, where it scales like lN, where N is the total system size. This EPT occurs for a system undergoing purely unitary evolution and does not involve measurements, postselection, disorder, or dissipation. We derive analytically the entanglement entropy out of and at the critical point for the cases of l=1 and l/N≪1.

Published by the American Physical Society
2024
Social media impact on international sports events related to the brand Spain: a comparison between inner versus outside events
Authors: Küster Boluda Inés , Vila-Lopez Natalia , Mora Elisabet , Casanoves-Boix Javier 
Published: January 2024

PurposeThis study analyzes the impact of sports social media on a country regarding three international events connected to the brand Spain. It examines (1) the use and importance of various social media platforms in sports events and (2) identifies the countries generating the most social media content.Design/methodology/approachA total of 1,711,084 posts were collected for analysis, focusing on FIFA World Cup Qatar 2022, XLI Marathon Valencia Trinidad Alfonso 2022 and Davis Cup 2022, with a particular emphasis on the Spain brand. Through Atribus, diverse social media data were recovered and analyzed. Later, we recommended employing various metrics and ANOVAs to address the research questions. Additionally, we conducted a sentiment analysis.FindingsThe results show differences between (1) the use and relevance of social network platforms and events and (2) the content generated by different countries. The practical implications offer valuable insights for sports event organizers, destination managers and other stakeholders. The research implications suggest potential avenues for future research based on the observed patterns and behaviors in social media posts related to sports events and Brand Spain.Originality/value(1) Some papers have studied the role of sports events’ social media, ignoring the comparison among different social media platforms; (2) usually, previous literature has focused on a single event or sport and (3) although there is considerable research related to the strategic and operational Inés Küster Boluda Inés Küster Boluda role of social media, there is less systematic analysis related to the extent sports events use social media in general and in specific social media platforms and virtually nonexistent studies that employ index measurements.
A qualitative evaluation of the questionnaire about the process of recovery (QPR) in culturally and linguistically diverse (CALD) populations
Authors: Kakuma Ritsuko , Cajethan Onah Uchenna , Shawyer Frances , Edan Vrinda , Wilson-Evered Elisabeth , Meadows Graham , Brophy Lisa 
Published: January 2024

Purpose
Mental health recovery across cultures lacks understanding and suitable measures. The Questionnaire about the Process of Recovery (QPR) is a self-report instrument measuring personal recovery outcomes for consumers of mental health services. However, the extent of its relevance among culturally and linguistically diverse (CALD) communities is unclear. This pilot study aimed to examine the relevance and utility of the QPR among CALD consumers of primary mental health services in Australia.

Design/methodology/approach
Eleven individual, semi-structured interviews were conducted with two general practitioners (GPs) and nine consumers from two clinics, at locations with high Iranian and Burmese refugee or asylum seeker populations. Interviews were transcribed and analysed using a thematic framework approach.

Findings
Although almost all consumers had little or no understanding of the concept of personal recovery, they found the QPR culturally acceptable and understandable. Using the QPR during mental health consultations can help with needs identification and goal setting. Challenges in using the QPR included completion time, cross-cultural differences in concepts and norms for some items, and need for careful translation. Consumers suggested additional items regarding family reputation, sexuality, and spirituality.

Originality/value
The QPR is potentially a valuable tool to support mental health consultations with CALD consumers, from the perspectives of both GPs and consumers.
Revisiting perceived gratification, consumer attitudes and purchase impulses in cross-border e-commerce live streaming: a direct and indirect effects model
Authors: Liu Renming , Abdul Hamid Abu Bakar , Ya'akub Noor Inayah 
Published: January 2024

Purpose
Cross-border e-commerce live streaming morphs into an epidemic shopping scenario, yet there is a paucity of research on impulse purchasing in this context. The purpose of this study is to empirically investigate the adoption motivation of cross-border e-commerce live streaming and its influence mechanism on intrinsic response and purchase impulse and to highlight the mediating role of browsing behavior.

Design/methodology/approach
Based on the use and gratification lens, a new conceptualization model is established to captivate the theoretical relationships between perceived stimuli, individual attitudes, browsing behavior and impulsive purchases. A questionnaire survey was used to collect cross-sectional data from 427 Malaysian consumers and the estimated framework was validated through AMOS-structural equation modeling technique.

Findings
The findings confirm that perceived interactivity, perceived information usefulness and perceived enjoyment significantly influenced positive attitudes toward live-streaming, which in turn induced impulsive purchases; however, perceived affective gratification did not stimulate positive attitudes. Consumers’ utilitarian browsing had a stronger effect on impulse purchases than hedonic browsing and utilitarian browsing behavior mediated the relationship between positive attitudes and impulse buying; however, hedonic browsing had neither a direct nor a mediating effect on impulsive purchases.

Practical implications
This research enhances the literature on the impact of cross-border e-commerce live streaming, an emerging technology, on consumer behavior and offers managerial implications for e-commerce practitioners to gain insights into consumer impulse purchasing behavior.

Originality/value
The findings revamp conventional knowledge and provide new angles for understanding the formation mechanisms of impulse purchases, motivations for virtual media use and browsing behavior mediating effects in the context of live streaming.
Whey protein and male acne: A double‐blind, randomized controlled trial
Authors: Sompochpruetikul Kittithorn , Khongcharoensombat Thanon , Chongpison Yuda , Rittirongwattana Wattanai , Asawanonda Pravit , Noppakun Nopadon , Kumtornrut Chanat 
Published: January 2024

AbstractBackgroundAcne vulgaris (AV) exacerbation after whey protein (WP) consumption remains inconclusive among experts.ObjectivesTo investigate the association between WP consumption and acne severity in men with acne.MethodsA noninferiority trial was conducted in men with mild to moderate facial and/or truncal acne. After randomization, participants in an intervention group took daily WP 30 g with a non‐WP nutritional supplement 18 g (WP group, n = 25), while the control group took a non‐WP nutritional supplement 46 g (non‐WP group, n = 24). At each follow‐up appointment, investigators evaluated acne count (total acne lesions, comedonal lesions, and inflammatory lesions) and severity.ResultsForty‐nine participants had a mean age of 19.7 years (standard deviation [SD], 0.9) and 20.3 years (SD, 1.4) in the WP and non‐WP groups, respectively. The mean differences in the facial and truncal total acne lesions for the WP and non‐WP group were −5.99 (95% confidence interval [CI], −13.18 to 1.19, p = 0.09) and −2.18 (95% CI, −11.83 to 7.48, p = 0.65), respectively. For severity changes, only one (4.3%) participant in the non‐WP group reported an increase in the Investigator Global Assessment scale of at least two levels.ConclusionsIn this 6‐month trial, men with acne who undertook WP supplementation showed a noninferior difference in the changes in total acne lesions and severity of facial and truncal acne compared with the non‐WP group.
Effectiveness of ixekizumab for the treatment of moderate‐to‐severe plaque psoriasis with involvement of difficult‐to‐treat areas: A 52‐week multicenter retrospective study
Authors: Valenti Mario , Gargiulo Luigi , Ibba Luciano , Cortese Andrea , Toso Francesco , Orsini Diego , Lora Viviana , Frascione Pasquale , Sena Paolo , Carugno Andrea , Assorgi Chiara , Costanzo Antonio , Narcisi Alessandra 
Published: January 2024

AbstractBiological drugs have dramatically changed the approach to treating moderate‐to‐severe plaque psoriasis, achieving excellent skin clearance and safety outcomes. However, the management of difficult‐to‐treat areas (e.g., scalp, palms/soles, nails, and genitalia) still represents a challenge in psoriasis treatment. Data in the literature on difficult‐to‐treat sites are limited and, frequently, no specific analysis is performed during clinical trials. We conducted a 52‐week, retrospective study to evaluate the effectiveness of ixekizumab in 120 patients with moderate‐to‐severe plaque psoriasis of at least one difficult‐to‐treat area (scalp, palmoplantar surfaces, nails, and genitalia). Ninety‐nine patients had scalp psoriasis, 35 had involvement of the palms or soles, 27 were affected by genital psoriasis, and 22 patients reported involvement of the nails. After 1 year of treatment, 96% of patients with scalp involvement, 95.6% of patients with palmoplantar psoriasis, 95.2% of patients with genital psoriasis, and 85% of patients with nail involvement achieved a site‐specific Physician's Global Assessment of 0 or 1 (clear or almost clear). No serious adverse events were observed during the study. Our study supports the effectiveness of ixekizumab in plaque psoriasis involving difficult‐to‐treat sites.
Cutaneous malignancies in chronic lymphocytic leukemia
Authors: Zilberg Catherine , Ferguson Angela L. , Lyons James G. , Gupta Ruta , Fuller Stephen J. , Damian Diona L. 
Published: January 2024

AbstractChronic lymphocytic leukemia (CLL) is a common lymphoid malignancy that is associated with an increased risk of developing cutaneous malignancies. Clinical outcomes for these malignancies, including melanoma and keratinocyte cancers (KC), are worse for patients with CLL. Individuals with CLL develop an immunodeficiency of both the adaptive and innate immune system, which plays a role in the increased prevalence of skin cancers. This review focuses on the complex interplay between genetics, immunity, and pathogens that influence the cellular composition and biology of skin tumors and their microenvironment in CLL patients, and in comparison with other chronic hematological malignancies. It is paramount for dermatologists to be aware of the association between CLL (and chronic hematological malignancies more broadly) and cutaneous malignancies. This is a high‐risk population who require regular and vigorous dermatologic follow‐up.