bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depression, Anxiety, and Anhedonia as Correlates of Distinct Family Functioning Domains among Adolescents
Authors: Nguyen Tram N. B. , Chance Aaron B. , Chase Emily , Rubinstein Tamar B. , Zayde Amanda , Roske Chloe , Mowrey Wenzhu , Gabbay Vilma 
Published: November 2023

Abstract

Background The bi-directional relationships between family dynamics and psychopathology have been documented, yet how aspects of family functioning relate to specific symptoms remains elusive. Such work is important in adolescence as it represents a vulnerable developmental period when psychiatric symptoms often emerge. Critically, most research on family functioning has focused on categorical clinical diagnoses and has not accounted for the inter-individual variability of symptom severity. Therefore, we aimed to study the associations between well-validated domains of family functioning and depression, anxiety, and anhedonia in youth using a dimensional approach. Methods We comprehensively evaluated 59 adolescents with diverse mood and anxiety symptoms and 17 controls with semi-structured diagnostic interviews and self-reported measures for depression, anxiety, and anticipatory and consummatory anhedonia. Adolescents’ perception of seven aspects of family life was assessed with the McMaster Family Assessment Device (FAD). Following bivariate assessments of associations between FAD subscales and symptom measures, linear regression models were employed to further investigate their relationships accounting for variations in age, sex, race, and ethnicity. Results were corrected for multiple comparisons. Results As hypothesized, more severe depression was implicated across most family domains, including Problem Solving, Communication, Roles, Affective Responsiveness, and General Functioning. Elevated anxiety was associated with worse family Problem Solving, Roles, and General Functioning. Interestingly, anhedonia showed no relationship with family domains following adjustment for multiple comparisons and depression severity, suggesting a different etiological pathway. Conclusions Our findings further support the intertwined relationships between family factors and adolescent mood processes and the need for specialized interventions addressing multiple family dimensions simultaneously.
‘QuickDASH’ to find Unique Genes and Biological Processes Associated with Shoulder Osteoarthritis: a prospective case-control study
Authors: Lynskey Samuel J. , Gill Stephen D. , McGee Sean L. , Ziemann Mark , Page Richard S. 
Published: November 2023

Abstract

Osteoarthritis (OA) is a disease of the synovial joint complex. A limited number of studies have directly investigated transcriptional changes in shoulder OA. Further, gene expression patterns of periarticular tissues in OA have not been thoroughly investigated. This prospective case control series details transcriptomic expression of shoulder OA by analysing periarticular tissues in patients undergoing shoulder replacement for OA as correlated with a validated patient reported outcome measure of worsening shoulder function, a higher (clinically worsening) QuickDASH score. We then compared transcriptomic expression profiles in capsular tissue biopsies from the OA group as compared to patients undergoing shoulder stabilisation for recurrent instability (the control group). Results indicated that top ranked genes associated with increasing QuickDASH score across all tissues involved inflammation and response to stress. These included interleukins, chemokines, complement components, nuclear response factors and immediate early response genes. Some of these genes were upregulated, and some downregulated, suggestive of a state of flux between inflammatory and anti-inflammatory signalling pathways. We have also described gene expression pathways in shoulder OA not previously identified in hip and knee OA, as well as novel genes involved in shoulder OA.
Caffeic acid phenethyl ester promotes oxaliplatin sensitization in colon cancer by inhibiting autophagy
Authors: Xing Fei , Liu Ning , Wang Can , Wang Xu-Dong 
Published: November 2023

Abstract
Colorectal cancer ranks as the third most prevalent form of cancer globally, with chemotherapy remaining the primary treatment modality. To mitigate drug resistance and minimize adverse effects associated with chemotherapy, selection of appropriate adjuvants assumes paramount importance. Caffeic acid phenyl ethyl(CAPE), a naturally occurring compound derived from propolis, exhibits a diverse array of biological activities. We observed that the addition of CAPE significantly augmented the drug sensitivity of colorectal cancer cells to oxaliplatin. In SW480 and HCT116 cells, oxaliplatin combined with 10 µM CAPE reduced the IC50 of oxaliplatin from 12 and 80 µM to 2 and 4 µM, respectively. We then used proteomics to detect differentially expressed proteins in CAPE-treated SW480 cells and found that the main proteins showing changes in expression after CAPE treatment were SQSTM1 (p62) and MAP1LC3B (LC3B). Gene ontology analysis revealed that CAPE exerted antitumor and chemotherapy-sensitization effects through the autophagy pathway. We subsequently verified the differentially expressed proteins using immunoblotting. Simultaneously, the autophagy inhibitor bafilomycin A1 and an mCherry-EGFP-LC3 reporter gene were used as controls to detect the effect of CAPE on autophagy levels. Collectively, the results indicate that CAPE may exert antitumor and chemotherapy-sensitizing effects by inhibiting autophagy, offering novel insights for the development of potential chemosensitizing agents.
The Effect of Race and Area Deprivation on Symptom Profiles over the Course of Early-Stage Breast Cancer
Authors: Abujaradeh Hiba , O'Brien Julia , Mazanec Susan R. , Bender Catherine M. , Schlemmer Isabelle M. , Brufsky Adam M. , Nasrollahi Elham , Rosenzweig Margaret 
Published: November 2023

Abstract

ABSTRACT
Purpose: This study compared common symptoms (fatigue, pain), overall physical functioning and changes over time between Black and White women receiving early-stage breast cancer (ESBC) chemotherapy.
Methods: A longitudinal, repeated measures comparative design was employed. Time points of symptom measurement (PROMIS domains) at baseline, mid and end point were adjusted as per patient chemotherapy schedule.
Analyses: Linear mixed models were applied.
Results: There were 147 patients, 36% Black 64% White (54±12 years) recommended to receive early-stage breast cancer chemotherapy with adequate data for symptom analysis.
Pain: Main effect of race was significant (F(1, 390) = 29.43, p<.001) for pain with Black patients experiencing significantly higher pain scores compared to White patients at pretherapy (Mean Difference; MD=3.7, p=.034), midpoint (MD=5.8, p=.002), and endpoint (MD=7.8, p<.001).
Fatigue: Fatigue significantly increased (deteriorated) at endpoint (MDT1-T3= 8.7, p<.001) for Black patients. Among White patients, fatigue significantly increased at midpoint (MDT1-T2= 5.7) and at endpoint (MDT1-T3=10.1, p<.001; MDT2-T3=4.3, p= .017). Physical function: Black patients had significantly lower physical function scores compared to White patients at midpoint (MD=4.0, p=.027). Physical function decreased by endpoint in Black (MDT1-T3=7.8, p<.001), and White patients (MDT1-T3=7.7, p<.001).
Conclusion: Symptom burden significantly increased over the course of chemotherapy for all patients. Scores for pain and physical function were higher overall for Black patients and deteriorated at a greater rate for Black vs. White women over the course of chemotherapy. This assessment holds implication for proactive assessment and mitigation strategies.
Evaluate the Efficacy of Guava-Derived CuO, and ZnO Nanocomposite Coating on Shelf life of Grapes
Authors: Singh Neha , Mittal Avneesh , Garg Meenakshi , Trivedi Rishabh , Kumar Pola Pavan , Sadhu Susmita Dey , Chopra Rajni 
Published: November 2023

Abstract
Pectin’s anionic heteropolysaccharide structure makes it suitable for coating metal nanoparticles (MPNs) by preventing their accumulation and thus, improves MPNs acceptability for formulation of edible coatings. The presence of functional moiety preferably, hydroxyl group and carboxylic acid in pectin promote their covalent bonding with metal nanoparticles, proteins, and other biomolecules. Accordingly, the present investigation explored the efficacy of guava-derived pectin-based zinc oxide (ZnO) and copper oxide (CuO) nanocomposite biopolymer coatings on the quality attributes and shelf-life extension of berries. The solvent casting and chemical reduction methods were used to synthesized guava-derived ZnO and CuO nanoparticles coatings, respectively. Simultaneously, berries submerged in three different biopolymer coatings viz., guava-derived pectin (GDP), GDP/ZnO and GDP/CuO for three minutes and stored at refrigerated temperature (i.e., 7oC) along with uncoated samples. The samples were evaluated for physicochemical changes and sensorial parameters at an interval of 2 days for 19 days of storage period and FTIR spectroscopy. The nanocomposite coatings were effective in reducing the berries' weight loss, water activity and minimized the reduction in pH, and titratable acidity. Comparably, an increase in total soluble solids (TSS) were observed in all coated samples but it was lower than uncoated ones. Furthermore, compared to controlled and other coated samples, only ZnO-coated berries had retained their texture, odor and were of acceptable quality to a lesser extent until 19 days of storage. The results of FTIR spectroscopy reveals the presence of alcohol and carboxylic acid group in the GDP structure. Supposedly, nanocomposite coatings could be harnessed as an active biopolymer packaging material that can prolong the lifespan and maintain the quality of grapes for an adequate period (at least two weeks) under refrigerated conditions.
MSCs play a vital function in Tibial cortex transverse transport surgery
Authors: Deng Baoping , Li Junjie , Weng Jiacong , Ge Xiaoyong , Xu Jing , Zheng Hongmei , Deng Weiping 
Published: November 2023

Abstract
Background
Tibial cortex transverse transport (TTT) has a good clinical effect in the treatment of diabetic foot, and the postoperative indexes of patients have been significantly improved. However, the mechanism of TTT on the increased expression of angiogenesis related factors and wound healing is still unclear
Methods
Diabetic foot model with tibial bone marrow purging was established to observe the effect of MSCs combined with TTT on angiogenesis and wound healing. The angiogenesis and wound healing process was evaluated by vascular perfusion, immunohistochemistry and Western blotting.
Results
After treatment with TTT surgery and MSCS injected into the myeloablated bone marrow cavity, the results showed faster wound healing and better skin recovery, angiography results showed that more new blood vessels were observe, and immunohistochemical staining and Western blotting detection also showed that TTT technology promoted CD31 expression through MSCs of the bone marrow cavity.
Conclusion
MSCs in the bone marrow cavity play a vital function in TTT surgery.
Lessons Learned: Establishing a CLIA-Equivalent Laboratory for Targeted Mass Spectrometry Assays – Navigating the Transition from Research to Clinical Practice
Authors: Han Chia-Li , Lai Chi-Ting , Reyes Aaron James , Yang Hao-Chin , Lu Jin-Ying , Shih Shyang-Rong , Chen Kuen-Yuan , Hoofnagle Andrew N , Yu Sung-Liang , Bocik William , Hiltke Tara , Chiu Huan-Chi , Wan Ching-Yi , Rodriguez Henry , Zhang Victoria , Chen Yu-Ju 
Published: November 2023

Abstract
Mass spectrometry (MS) assays offer exceptional capabilities in high multiplexity, specificity, and throughput. As proteomics technologies continue advancements to identify new disease biomarkers, transition of these innovations from research settings to clinical applications becomes imperative. To meet the rigorous regulatory standards of clinical laboratories, development of a clinical protein MS assay necessitates adherence to stringent criteria. To illustrate the process, this project focused on using thyroglobulin (Tg) as a biomarker and an immuno-multiple reaction monitoring (iMRM) MS-based assay as a model for establishing a Clinical Laboratory Improvement Amendments (CLIA) compliant laboratory within the Centers of Genomic and Precision Medicine, National Taiwan University. The chosen example also illustrates the clinical utility of MS assays to complement conventional immunoassay-based methods, particularly in cases where the presence of autoantibodies in 10–30% of patients hinders accuracy. The laboratory design entails a comprehensive coordination in spatial layout, workflow organization, equipment selection, ventilation systems, plumbing, electrical infrastructure, documentation procedures, and communication protocols. Practical aspects of the transformation process, including preparing laboratory facilities, testing environments, instrument validation, assay development and validation, quality management, sample testing, and personnel competency, are discussed. Finally, concordant results in proficiency testing demonstrate the harmonization with the University of Washington Medical Center and the quality assurance of the CLIA-equivalent Tg-iMRM MS assay established in Taiwan. The realization of this model protein MS assay in Taiwan highlights the feasibility of international joint development and provides a detailed reference map to expedite the implementation of more MS-based protein assays in clinical laboratories for patient care.
Efficacy of a stable multivalent broadly protective subunit vaccine platform against SARS-CoV-2 variants of concern
Authors: Banerjee Arinjay , Garg Ravendra , Liu Qiang , Kessel Jill van , Asavajaru Akarin , Uhlemann Eva-Maria , Joessel Morgane , Hamonic Glenn , Khatooni Zahed , Kroeker Andrea , Lew Jocelyne , Scruten Erin , Pennington Paul , Deck William , Prysliak Tracy , Nickol Michaela , Apel Falko , COURANT Thomas , kelvin Alyson , Kessel Andrew Van , COLLIN Nicolas , Gerdts Volker , Köster Wolfgang , Falzarano Darryl , Racine Trina 
Published: November 2023

Abstract
The emergence and ongoing evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the need for rapid vaccine development platforms that can be updated to counteract emerging variants of currently circulating and future emerging coronaviruses. Here we report the development of a “train engine and car” subunit vaccine platform that contains a SARS-CoV-2 Wuhan S1 protein linked to a series of flexible receptor binding domains (RBDs) derived from SARS-CoV-2 variants of concern (VOCs). We demonstrate that these linked subunit vaccines when combined with the SWE adjuvant are immunogenic in Syrian hamsters and subsequently provide protection from challenge with SARS-CoV-2 VOCs Omicron, Delta, and Beta. Importantly, the bivalent and trivalent vaccine candidates offered protection against some heterologous SARS-CoV-2 VOCs that were not included in the vaccine design, demonstrating the potential for broad protection against a range of different VOCs. Furthermore, these formulated vaccine candidates were stable at 2–8 °C for up to 12 months post-formulation, highlighting their utility in low-resource settings. Indeed, our vaccine platform will enable the development of safe and broadly protective vaccines against emerging betacoronaviruses that pose a significant health risk for humans and agricultural animals.
Anti-arthritic activity of Pogostemon quadrifolius
Authors: Madhiri Ramya , Barik Rakesh 
Published: November 2023

Abstract
Aim of the study: To investigate anti-arthritic activity of Aqueous methanolic extract of Pogostemon quadrifolius (AMEPQ) in Freund’s complete adjuvant (FCA)-induced arthritis in rats.
Methods: The AMEPQ was prepared and subjected to acute oral toxicity in mice and tested against FCA induced arthritis in rats. Arthritis assessment was done by measuring – paw volume, joint diameter, pain threshold, thermal hyperalgesia, mechanical nociceptive threshold and body weight.
Haematological, serum, biochemical and in vivo anti-oxidant parameters were measured on the last day of the study. Histopathological and radiological analyses of ankle joints were also done. MEPQ was administered at the dose of 100, 200 and 400 mg/kg body weight.
Results: MEPQ dose dependently showed anti-arthritic activity which was evident with decrease in paw volume, joint diameter and increase in pain threshold, paw withdrawal latency, mechanical nociceptive threshold and body weight when compared to arthritic control group. AMEPQ (400 and 200 mg/kg) exhibits signiﬁcant (P < 0.001 and P < 0.01, respectively) anti-arthritic activity by increasing levels of RBC, Hb and by decreasing levels of WBC, platelets and also serum C-reactive protein (CRP) and Rheumatoid factor (RF). The anti-arthritic activity was also conﬁrmed with the altered biochemical parameters (AST, ALT, ALP and total protein level) and anti-oxidant parameters (SOD, MDA and GSH). MEPB (400 and 200 mg/kg) and diclofenac (10 mg/ kg) also inhibited joint destruction (histopathological and radiological analysis).
Conclusion: P. quadrifolius may be a potential preventive or therapeutic candidate for the treatment of inﬂammation and arthritis.
Impact of Long-COVID on Cardiac Arrhythmias: A Multi-Center Study from the Middle Euphrates Region, Iraq
Authors: Sadiq Alaa M. , Al-Amran Fadhil 
Published: November 2023

Abstract

Long-COVID, characterized by prolonged symptoms following an acute COVID-19 infection, has emerged as a significant public health concern. Cardiac arrhythmias are among the critical sequelae associated with long-COVID. This study aims to assess the incidence and nature of cardiac arrhythmias in long-term patients. This retrospective cohort study involved 340 patients diagnosed with long-term COVID-19, recruited from various hospitals in the Middle Euphrates region throughout 2022. The majority of the cohort were male, with ages ranging from 25 to 89 years. Data on cardiac arrhythmias were collated and analyzed. The study found a notable incidence of cardiac arrhythmias in long-COVID-19 patients, with variations across different age groups and a predominance in male patients. The types of arrhythmias and their severity differed significantly, indicating the diverse impact of long-term on cardiac health. The findings suggest a significant association between long-COVID-19 and the development of cardiac arrhythmias. The study highlights the need for vigilant cardiac monitoring in long-COVID-19 patients and underscores the importance of further research to understand the underlying mechanisms and to develop effective management strategies.
Long-Term COVID-19 and Its Accelerating Impact on Atherosclerotic Cardiovascular Diseases in Iraq
Authors: Sadiq Alaa M. , Al-Amran Fadhil 
Published: November 2023

Abstract

Background:The COVID-19 pandemic's long-term effects on cardiovascular health, particularly regarding atherosclerotic diseases, are of increasing concern.Methods:In this retrospective cohort study, 450 patients from various Iraqi provinces who had recovered from COVID-19 were assessed over 12 months to evaluate the prevalence and severity of atherosclerotic cardiovascular diseases (CVD).Results:There was a significant increase in atherosclerotic changes among patients with a history of long-term COVID-19 compared to those without. A notable correlation was observed between the severity of post-COVID symptoms and the extent of atherosclerotic manifestation, across different demographic groups and geographical locations within Iraq.Conclusions:The study concludes that long-term COVID-19 may contribute to the accelerated development of atherosclerotic CVD. These findings underscore the need for enhanced cardiovascular monitoring and intervention strategies in patients recovering from COVID-19, particularly those with severe or prolonged symptoms.
Polyethylene Glycol and Caspase Inhibitor Emricasan Alleviates Cold Injury in Primary Rat Hepatocytes
Authors: Chen Huyun , Ellis Bradley W. , Dinicu Antonia T. , Mojoudi Mohammadreza , Wilks Benjamin T. , Tessier Shannon N. , Toner Mehmet , Uygun Korkut , Uygun Basak E. 
Published: November 2023

Abstract

Current methods of storing explanted donor livers at 4°C in University of Wisconsin (UW) solution result in loss of graft function and ultimately leads to less-than-ideal outcomes post transplantation. Our lab has previously shown that supplementing UW solution with 35-kilodalton polyethylene glycol (PEG) has membrane stabilizing effects for cold stored primary rat hepatocytes in suspension. Expanding on past studies, we here investigate if PEG has the same beneficial effects in an adherent primary rat hepatocyte cold storage model. In addition, we investigated the extent of cold-induced apoptosis through treating cold-stored hepatocytes with pan caspase inhibitor emricasan. In parallel to storage at the current cold storage standard of 4°C, we investigated the effects of lowering the storage temperature to –4°C, at which the storage solution remains ice-free due to the supercooling phenomenon. We show the addition of 5% PEG to the storage medium significantly reduced the release of lactate dehydrogenase (LDH) in plated rat hepatocytes and a combinatorial treatment with emricasan maintains hepatocyte viability and morphology following recovery from cold storage. These results show that cold-stored hepatocytes undergo multiple mechanisms of cold-induced injury and that PEG and emricasan treatment in combination with supercooling may improve cell and organ preservation.
Investigations of the shallow bedrock using the seismic refraction method – A case study in New Qena City, Eastern Desert, Egypt
Authors: Gowad Ahmed M. Abdel , El-Haddad Assem E. , Aglan Mohamed I. , Hamed Ahmed 
Published: November 2023

Abstract
The seismic refraction technique has demonstrated its efficiency as a cost-effective geophysical approach for bedrock investigation, which is very important for major construction projects. In the eastern part of New Qena City, along Qena-Safaga Road in the Eastern Desert of Egypt, construction of many domestic facilities is planned. Therefore, a prior site investigation focusing on bedrock is required to validate the site for construction and other projects. In this study, a site investigation of the eastern part of New Qena City is conducted using the seismic refraction method to estimate the depth and thickness of the bedrock and to present the surface structural features affecting the area. Ten seismic refraction profiles were measured in the area. The results of tomographic modeling show that the bedrock consists of three layers, with the bedrock represented by the mudstone unit of the Pliocene Durri Formation. This layer is located at a shallow depth and varies in thickness in the area. The seismic velocity sections show that the layer is characterized by relatively low velocities as well as lateral velocity variations. This is interpreted to be due to the clay content of the layer, the high degree of fracturing, and the lateral facies variation. Therefore, the layer could undergo geotechnical problems that could affect future construction projects in the area. Four subsurface faults were also detected in the velocity sections created by the tomographic inversion.