bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metaphors as design tools for microbial consortia: An analysis of recent peer‐reviewed literature
Authors: Szymanski Erika Amethyst , Turner Marie 
Published: November 2023

AbstractSingle engineered microbial species cannot always conduct complex transformations, while complex, incompletely defined microbial consortia have heretofore been suited to a limited range of tasks. As biodesigners bridge this gap with intentionally designed microbial communities, they will, intentionally or otherwise, build communities that embody particular ideas about what microbial communities can and should be. Here, we suggest that metaphors—ideas about what microbial communities are like—are therefore important tools for designing synthetic consortia‐based bioreactors. We identify a range of metaphors currently employed in peer‐reviewed microbiome research articles, characterizing each through its potential structural implications and distinctive imagery. We present this metaphor catalogue in the interest of, first, making metaphors visible as design choices, second, enabling deliberate experimentation with them towards expanding the potential design space of the field, and third, encouraging reflection on the goals and values they embed.
Comparing different citizen science platforms for collecting urban ecological data from Toco toucan (Ramphastos toco) in Argentina
Authors: Schaaf Alejandro A. , Haag Laura M. , Gonzalez Baffa‐Trasci Noelia V. , Yapura Agustina , Chocobar Natalia , Caldano Sol A. , Ruggera Román A. 
Published: November 2023

AbstractCitizen science projects are valuable tools since they provide substantial amounts of data on different animal species. Currently, there are a wide variety of platforms and objectives for diverse citizen science projects. Despite these advances, the approaches and contributions of citizen science platforms for collecting data on a particular species, and in a specific site or region, have been poorly explored. Thus, in this article, we compare the number of participants and the amount of data collected by three platforms on the Toco toucan (Ramphastos toco) in San Salvador de Jujuy city, located in northwestern Argentina. This particular bird species is very charismatic and frequents urban areas all year round; so that, it is interesting to know what contributions (e.g. participant numbers, ecological and distribution data) are being made about this species on citizen science platforms. Thus, we examined data reported by two global citizen science platforms, iNaturalist and eBird, and one local platform, Tucanes en mi Jujuy (TEMJ). We found that the local, focal TEMJ project had a greater number of participants (n = 193), greater presence records (n = 614) and a wide spatial distribution of data in the city. In the iNaturalist and eBird platforms, the number of participants was smaller (n = 9 and 36, respectively), with fewer presence records (n = 12 and 178, respectively), and even fewer reports of species‐specific ecological data (feeding, behaviour and age). This reflected clear differences between the three platforms, as TEMJ not only reported more presence data but also more detailed information on age and behaviour. Based on this research, we highlight the commitment of citizens to this particular project and the study of species. Therefore, we recommend the implementation of new local and focal citizen science platforms for more detailed ecological data on other charismatic and focal species.
Preparation of betaine injection and its therapeutic effect in pulmonary arterial hypertension
Authors: Che Youlan , Xia Tian , Liu Hui , Li Cong , Liu Siyun , Ma Ping , Xu Qingbin , Zhou Ru 
Published: November 2023

AbstractPulmonary arterial hypertension (PAH) is a life‐threatening disease characterised by elevated pulmonary pressure, right ventricular failure (RVF) and ultimately death. Aggressive treatment of RVF is considered an important therapeutic strategy to treat PAH. Previous studies have indicated that betaine may be may a promising therapeutic approach for PAH‐induced RVF. Therefore, in this study, betaine solution for injection was prepared and characterised using various techniques. The therapeutic efficacy of three different methods of administration (intragastric, nebulised inhalation and intravenous injection) were comprehensively evaluated in terms of pharmacokinetics, tissue distribution and pharmacodynamics. The pharmacokinetic results demonstrated that betaine injection administered via nebulised inhalation significantly prolonged betaine's half‐life and increased its internal circulation time compared to the intragastric and intravenous routes. Biodistribution experiments verified that the betaine formulation accumulated in the lung tissue when administered via inhalation. The results of the pharmacodynamic analysis further confirmed that right ventricular systolic pressure, mean pulmonary artery pressure and right ventricular hypertrophy index increased in the model group and that inhaled betaine suppressed these pathological changes to a level comparable to those observed in the control group. Taken together, these results indicate that betaine administered by inhalation is a promising strategy for the treatment of PAH‐induced RVF.
A causeway to impact: A proposed new integrated framework for intergenerational community‐based participatory action research
Authors: Loudon Emma , Neary Joanne , McAteer Ben , Higgins Kathryn , Chapman Christopher 
Published: November 2023

AbstractOver recent decades there has been growing interest in amplifying children and young people's views (CYP) within policy debates. Despite this, they are rarely invited to participate in key policy‐making discussions, and when they are, this tends to be tokenistic. This paper presents an intergenerational methodological framework ‘The Causeway Approach’, inspired by the mythology of the Giant's Causeway, which addresses the challenge of CYP's voices being drowned out by adult stakeholders. This contextualised approach has significant potential to benefit CYP and communities through capacity building, strengthening of social capital and fostering intergenerational connections.
Microbial mats and their palaeoenvironmental analysis in offshore – shelf facies of the Los Molles Formation (Toarcian – Lower Callovian) in the Chacay Melehue area, Neuquén Basin, Argentina
Authors: Rodriguez Maximiliano Nicolás , Campetella Débora Mical , Carmona Noelia Beatriz , Ponce Juan José , Parada Martín Nazareno 
Published: November 2023

AbstractThis contribution presents the first study focused on the analysis of microbial mats in the Los Molles Formation (Toarcian – Early Callovian), Neuquén Basin, Argentina. This unit mainly represents offshore‐to‐shelf environments affected by storms and density currents. The Los Molles Formation is one of the oldest source rocks in the Neuquén Basin and constitutes an unconventional shale gas reservoir of great economic importance. The aim of this work was to identify the microbial activity from the description and interpretation of microbially induced sedimentary structures (MISS), to determine the paleoenvironmental and paleoecological conditions under which they formed, and to establish a possible relationship between these structures and the trace fossil Trichichnus. Samples from the levels with MISS were analyzed and described from macroscopic and binocular observations, petrographic microscope thin sections, and SEM samples with EDS analyses. The results showed several levels of microbial mats presenting diverse MISS, including biolaminations and Kinneyia‐like wrinkles structures that were described at the macroscopic level. In thin sections, biolaminations, filament‐like microstructures with different degrees of development, oriented grains and pyrite were observed. SEM images and EDS analyses showed different types of filaments, coccoids and EPS with high concentrations of carbon. These results revealed that the studied levels fulfill the established biogenicity criteria, guaranteeing that they have a bacterial origin. The abundance of the trace fossil Trichichnus sp. throughout the section and the proximity to some Kinneyia‐like wrinkle structures levels suggests that the same organisms may have generated them. Furthermore, they revealed that the Los Molles Formation, at the time of its deposition, experienced paleoecological and paleoenvironmental conditions appropriate for the establishment and development of microbial mats. The extensive levels of microbial mats in the study area suggest that they may have been a source of organic matter for the generation of hydrocarbons from the Los Molles Formation.
State of the Practice Worldwide: Utilizing Hydrogeochemical Data and GIS Tools to Assess the Groundwater Quality in Arid Region: Example from Wadi Feiran Basin, Southwestern Sinai, Egypt
Authors: Omar Ali E. , Abdel‐Halim Khaled A. , Arnous Mohamed O. 
Published: November 2023

AbstractThe paper aims to study the physio‐chemical parameters of Wadi Feiran groundwater and classify it for use for drinking and irrigation. Twenty water samples collected from the WF have revealed a number of physio‐chemical parameters such as pH, TDS, SAR, EC, ions (Na+, Mg+2, Ca+2, K+, Cl−, SO4−2, NO3−, and HCO3−), and trace elements (U, Pb, Cd, Zr, and Fe). The study clarified that the major ion parameters are within the permissible limits according to WHO 2018, except for Ca+2, Cl−, SO4−2, and NO3−, but trace elements have high concentrations, except for Zn. To recognize the association between groundwater quality parameters and their collective impact on whole groundwater quality, correlation coefficient, factor, and cluster multivariate statistical analyses were carried out and confirmed with the constructed LU/LC map. These revealed that the increase of TDS, EC, Cl, SO4, Na, Mg, and NO3 is attributed to rainfall, leakage of municipal sewage Nile water, and human actions. GIS approaches were used to create thematic layers from groundwater physio‐chemical parameters, which were then weighted and combined to produce a groundwater quality model. This model was classified into potable, marginal, and un‐potable water. Potable and marginal water can be used for drinking and irrigation, but un‐potable water can only be used for irrigation.
SEQUENTIAL PRICE SETTING: THEORY AND EVIDENCE FROM A LAB EXPERIMENT
Authors: Heggedal Tom‐Reiel , Helland Leif , Moen Espen R. 
Published: November 2023

AbstractIn the Varian (1980; American Economic Review 70(4) (1980), 651–59) model of price competition, a change from simultaneous to sequential price setting dramatically changes equilibrium strategies, and in the unique symmetric, equilibrium prices are pushed up to the monopoly price. There also exists an asymmetric equilibrium with lower average prices. Our main contribution is to test these predictions in the laboratory. Our data strongly support the qualitative model predictions. However, a fraction of players set low prices in accordance with the asymmetric equilibrium, which is puzzling. We show that the puzzle to a large extent can be resolved by introducing competitive preferences in the model.
A comparative analysis of chronic inducible urticaria in 423 patients: Clinical and laboratory features and comorbid conditions
Authors: Ornek Ozdemir S. , Kuteyla Can P. , Degirmentepe E. N. , Cure K. , Singer R. , Kocaturk E. 
Published: November 2023

AbstractBackgroundChronic inducible urticaria (CIndU) is a subtype of chronic urticaria (CU) which require specific physical or non‐physical triggers to occur. They may be isolated or may coexist with chronic spontaneous urticaria (CSU). Despite their frequent appearance in dermatology clinics, there is scarce information on the distinguishing features among the most common subtypes of CIndU as well as isolated CIndU versus CSU plus CIndU.ObjectivesTo compare clinical and laboratory characteristics, and comorbid conditions among the most common CIndU types and isolated CIndU versus CSU plus CIndU.MethodsWe retrospectively analysed CIndU patients and compared patients' demographic, clinical and laboratory characteristics across isolated CIndU, CSU plus CIndU, symptomatic dermographism (SD), cold urticaria (ColdU) and cholinergic urticaria (ChoU).ResultsA total of 423 patients (~70% isolated CIndU, ~30% CSU plus CIndU, ~5% mixed CIndU subtypes) were included in the study. The most frequent CIndU subtypes were SD (68.6%; 290/423), ColdU (11.4%; 48/423) and ChoU (10.9%; 46/423). Isolated CIndU patients were younger than CSU plus CIndU (33.74 ± 12.72 vs. 37.06 ± 11.84, p = 0.010). Angioedema, emergency referrals, need for systemic steroids, comorbid systemic disorders were more frequent and baseline urticaria control test scores were lower in CSU plus CIndU patients (vs. CIndU, p < 0.001, p = 0.008, p < 0.001, p = 0.031, p = 0.036, respectively). Among CIndU subtypes, ChoU patients were younger (24.9 ± 12.2 vs. 34.47 ± 12.12 vs. 31.38 ± 14.95; p < 0.001) and had male predominance (p < 0.001) while SD patients had no angioedema (p < 0.001) and had higher frequency of increased total IgE levels (p = 0.006).ConclusionsIsolated CIndU and CSU plus CIndU seems to be different endotypes of CU where CSU plus CIndU presents a more severe and refractory course. There are distinctive features of each CIndU subtype. These suggest involvement of different pathomechanistic pathways in these subtypes that need to be clarified in future studies.
Prediction of duloxetine efficacy in addition to self‐management in painful temporomandibular disorders: A randomised, placebo‐controlled clinical trial
Authors: Ferreira Dyna Mara Araújo Oliveira , Soares Flávia Fonseca Carvalho , Raimundini Amanda Ayla , Bonjardim Leonardo Rigoldi , Costa Yuri Martins , Conti Paulo César Rodrigues 
Published: November 2023

AbstractBackgroundConditioned pain modulation (CPM) is a potential predictor of treatment response that has not been studied in temporomandibular disorders (TMD).ObjectivesWe conducted a randomised, double‐blind, placebo‐controlled trial (RCT) of duloxetine in addition to self‐management (SM) strategies to investigate its efficacy to reduce pain intensity in painful TMD patients. Moreover, we investigated whether baseline CPM would predict the duloxetine efficacy to reduce TMD pain intensity.MethodsEighty participants were randomised to duloxetine 60 mg or placebo for 12 weeks. The primary outcomes were the change in the pain intensity from baseline to week‐12 and CPM‐sequential paradigm at baseline. Safety, physical and emotional functioning outcomes were also evaluated.ResultsOf 80 participants randomised, 78 were included in intention‐to‐treat analysis. Pain intensity decreased for SM‐duloxetine and SM‐placebo but did not differ between groups (p = .82). A more efficient CPM was associated with a greater pain intensity reduction regardless of the treatment group (p = .035). Physical and emotional functioning did not differ between groups, but adverse events (p = .014), sleep impairment (p = .003) and catastrophizing symptoms (p = .001) were more prevalent in SM‐duloxetine group.ConclusionThis study failed to provide evidence of a beneficial effect of adding duloxetine to SM strategies for treatment of painful TMD. Nonetheless, this RCT has shown the feasibility of applying pain modulation assessment to predict short‐term treatment response in painful TMD patients, which confirms previous finds that CPM evaluation may serve a step forward in individualising pain treatment.
L2 Learners’ Signed Language Processing Relates, in Part, to Perspective‐Taking Skills
Authors: Quinto‐Pozos David , Renee Joyce Taylor , Sarkar Abhra , DiLeo Michael , Hou Lynn 
Published: November 2023

AbstractThe comprehension of signed language requires linguistic and visual–spatial processing, such as perspective‐taking for correctly interpreting the layout of a spatial scene. However, little is known about how adult second‐language (L2) learners process visual–spatial constructions in a signed language that they are studying, including which angles of viewing are most challenging to process and whether there are relationships between perspective‐taking and the comprehension of non‐spatial (i.e., non‐scene based) constructions. We examine the performance of 95 intermediate signers of American Sign Language (ASL) on linguistic and non‐linguistic perspective‐taking tests. Half the participants completed a test of narrative comprehension that included visual–spatial scenes, and half took a test of signed phonological and morphophonological discrimination. Performance on linguistic perspective‐taking correlated moderately with performance on the narrative, but not with the discrimination test. These findings support the claim that perspective‐taking skills are yoked to some—but not all—aspects of signed language learning.
Mineralogy and petrology of fine‐grained samples recovered from the asteroid (162173) Ryugu
Authors: Noguchi Takaaki , Matsumoto Toru , Miyake Akira , Igami Yohei , Haruta Mitsutaka , Saito Hikaru , Hata Satoshi , Seto Yusuke , Miyahara Masaaki , Tomioka Naotaka , Ishii Hope A. , Bradley John P. , Ohtaki Kenta K. , Dobrică Elena , Leroux Hugues , Guillou Corentin Le , Jacob Damien , de la Peña Francisco , Laforet Sylvain , Mouloud Bahae‐Eddine , Marinova Maya , Langenhorst Falko , Harries Dennis , Beck Pierre , Phan Thi H. V. , Rebois Rolando , Abreu Neyda M. , Gray Jennifer , Zega Thomas , Zanetta Pierre‐M. , Thompson Michelle S. , Stroud Rhonda , Burgess Kate , Cymes Brittany A. , Bridges John C. , Hicks Leon , Lee Martin R. , Daly Luke , Bland Phil A. , Smith William A. , McFadzean Sam , Martin Pierre‐Etienne , Bagot Paul A. J. , Fougerouse Dennis , Saxey David W. , Reddy Steven , Rickard William D. A. , Zolensky Michael E. , Frank David R. , Martinez James , Tsuchiyama Akira , Yasutake Masahiro , Matsuno Junya , Okumura Shota , Mitsukawa Itaru , Uesugi Kentaro , Uesugi Masayuki , Takeuchi Akihisa , Sun Mingqi , Enju Satomi , Takigawa Aki , Michikami Tatsuhiro , Nakamura Tomoki , Matsumoto Megumi , Nakauchi Yusuke , Abe Masanao , Nakazawa Satoru , Okada Tatsuaki , Saiki Takanao , Tanaka Satoshi , Terui Fuyuto , Yoshikawa Makoto , Miyazaki Akiko , Nakato Aiko , Nishimura Masahiro , Usui Tomohiro , Yada Toru , Yurimoto Hisayoshi , Nagashima Kazuhide , Kawasaki Noriyuki , Sakamotoa Naoya , Hoppe Peter , Okazaki Ryuji , Yabuta Hikaru , Naraoka Hiroshi , Sakamoto Kanako , Tachibana Shogo , Watanabe Sei‐ichiro , Tsuda Yuichi 
Published: November 2023

AbstractSamples returned from the carbonaceous asteroid (162173) Ryugu by the Hayabusa2 mission revealed that Ryugu is composed of materials consistent with CI chondrites and some types of space weathering. We report detailed mineralogy of the fine‐grained Ryugu samples allocated to our “Sand” team and report additional space weathering features found on the grains. The dominant mineralogy is composed of a fine‐grained mixture of Mg‐rich saponite and serpentine, magnetite, pyrrhotite, pentlandite, dolomite, and Fe‐bearing magnesite. These grains have mineralogy comparable to that of CI chondrites, showing severe aqueous alteration but lacking ferrihydrite and sulfate. These results are similar to previous works on large Ryugu grains. In addition to the major minerals, we also find many minerals that are rare or have not been reported among CI chondrites. Accessory minerals identified are hydroxyapatite, Mg‐Na phosphate, olivine, low‐Ca pyroxene, Mg‐Al spinel, chromite, manganochromite, eskolaite, ilmenite, cubanite, polydymite, transjordanite, schreibersite, calcite, moissanite, and poorly crystalline phyllosilicate. We also show scanning transmission electron microscope and scanning electron microscope compositional maps and images of some space‐weathered grains and severely heated and melted grains. Although our mineralogical results are consistent with that of millimeter‐sized grains, the fine‐grained fraction is best suited to investigate impact‐induced space weathering.
Testing the trade‐balance model: resource stoichiometry does not sufficiently explain AM effects
Authors: Corrêa Ana , Ferrol Nuria , Cruz Cristina 
Published: November 2023

Summary

Variations in arbuscular mycorrhizae (AM) effects on plant growth (MGR) are commonly assumed to result from cost : benefit balances, with C as the cost and, most frequently, P as the benefit. The trade‐balance model (TBM) adopts these assumptions and hypothesizes that mycorrhizal benefit depends on C : N : P stoichiometry. Although widely accepted, the TBM has not been experimentally tested.

We isolated the parameters included in the TBM and tested these assumptions using it as framework. Oryza sativa plants were supplied with different N : P ratios at low light level, establishing different C : P and C : N exchange rates, and C, N or P limitation. MGR and effects on nutrient uptake, %M, ERM, photosynthesis and shoot starch were measured.

C distribution to AM fungi played no role in MGR, and N was essential for all AM effects, including on P nutrition. C distribution to AM and MGR varied with the limiting nutrient (N or P), and evidence of extensive interplay between N and P was observed.

The TBM was not confirmed. The results agreed with the exchange of surplus resources and source–sink regulation of resource distribution among plants and AMF. Rather than depending on exchange rates, resource exchange may simply obey both symbiont needs, not requiring further regulation.
Macrophage‐derived apoptotic bodies impair the osteogenic ability of osteoblasts in periodontitis
Authors: Liu Xu , Guo Lijia , Du Juan , Luo Zhenhua , Xu Junji , Bhawal Ujjal Kumar , Li Xiaoyan , Liu Yi 
Published: November 2023

AbstractObjectivesPeriodontitis is induced by the imbalance between osteoblast and osteoclast activity, which leads to periodontal tissue destruction. Macrophages play a vital role in periodontitis. However, the hypoxic periodontal environment will also induce macrophage apoptosis within a short time. Apoptotic bodies (ABs) are the major products generated from apoptotic cells, but whether macrophage‐derived ABs play a regulatory role as their mother cells in periodontitis remains unknown. In the present study, we aimed to investigate the effects of ABs on osteoblasts.MethodABs derived from hypoxia‐induced macrophages were co‐cultured with osteoblasts and the impact of ABs on osteoblast differentiation in vitro was assessed. In vivo, periodontitis model was established and macrophages‐derived ABs were injected into the gingival sulcus. The effects of ABs on periodontal bone resorption were determined.ResultsThe results showed that ABs significantly inhibit osteoblast differentiation and promoted alveolar bone resorption in periodontitis. MicroRNA (miRNAs) array analysis was performed and revealed that miR‐483‐5p is the key miRNA in ABs. Dual luciferase reporter assays were performed and confirmed that miR‐483‐5p targeted Col1A1 mRNA and attenuated its expression.ConclusionMacrophage‐derived ABs inhibit osteoblast differentiation via the transfer of miR‐483‐5p, which downregulates Col1A1 expression and finally suppresses osteogenic activity.