bluuebunny/crossref_metadata_2025_split huggingface dataset:
MARK3-mediated Slingshot-1 phosphorylation is essential for polarized lamellipodium formation
Authors: Mishima Toshiaki , Ohta Yusaku , Ohashi Kazumasa , Mizuno Kensaku 
Published: October 2023

ABSTRACTCofilin acts as a key regulator of actin cytoskeletal remodeling via stimulating actin filament disassembly. Cofilin is inactivated by Ser-3 phosphorylation and reactivated by cofilin-phosphatase Slingshot-1 (SSH1). SSH1 is activated upon binding to F-actin, and this activation is inhibited by its phosphorylation at Ser-937 and Ser-978 and the subsequent binding of 14-3-3 proteins. In this study, we identified MARK3 (also named Par-1a and C-TAK1) as a kinase responsible for Ser-937/Ser-978 phosphorylation of SSH1. MARK3-mediated phosphorylation promoted SSH1 binding to 14-3-3 proteins and suppressed its F-actin-assisted cofilin-phosphatase activity. When Jurkat cells were stimulated with SDF-1α, actin filaments formed multidirectional F-actin-rich lamellipodia around the cells in the initial stage, and thereafter, they were rearranged as a single polarized lamellipodium to the direction of cell migration. Upon SDF-1α stimulation, SSH1 was translocated into F-actin-rich lamellipodia, but its Ser-937/Ser-978 non-phosphorylatable mutant SSH1(2SA) was retained at the location of the original cortical F-actin. Knockdown of MARK3 or overexpression of SSH1(2SA), similar to SSH1 knockdown, impaired the conversion of multiple lamellipodia to a single polarized lamellipodium. These results indicate that MARK3-mediated Ser-937/Ser-978 phosphorylation is required for SSH1 liberation from F-actin and translocation to lamellipodia, and hence, facilitates the formation of a single polarized lamellipodium for directional cell migration. Our results suggest that the phosphorylation-dephosphorylation cycle of SSH1 is crucial for its localization to lamellipodia via promoting the dissociation-reassociation cycle of SSH1 to F-actin, and thereby the stimulus-induced lamellipodium formation to the direction of cell movement.
Plakins are involved in the maintenance of epithelial polarity
Authors: Geay Juliana , Margaron Yoran , Gentien David , Reyal Fabien , Puisieux Alain , Blanchoin Laurent , Guyon Laurent , Théry Manuel 
Published: October 2023

AbstractAs epithelia are the interface between the organism and the external environment, they are often subject to damage and must be frequently renewed. However, maintaining epithelial integrity during this renewal is challenging, and loss of cell polarity is a potent inducer of tumorigenesis. In this study, we used transcriptomic data from breast cancer cells at different stages of tumor development to identify molecular changes associated with the early stages of tumor transformation. We correlated these protein expression profiles with either cell polarity defects or cell progression along the epithelial-to-mesenchymal transition (EMT). We identified plakins, namely epiplakin (EPPK1), desmoplakin (DSP) and periplakin (PPL), that were downregulated in cells that had lost their epithelial polarity and also downregulated in cells that had progressed through EMT. We further tested them experimentally by knocking down their expression in a non-tumorigenic epithelial breast cell line (MCF10A). We demonstrated their causal role in the loss of polarity, as revealed by the misorientation of the nuclear centrosome vector. We also found that vimentin, a marker of EMT, was overexpressed in plakin knocked-down cells, suggesting that plakins may have both a structural and a regulatory role in maintaining the epithelial state.
The effect of human educational capital on health entitlement acquisition among migrant workers in China: evidence from the 2018 China Migrants Dynamic Survey
Authors: Chen Hong , Chen Chien-Ping , Jin Wang , Wang Yangyang , Qin Lijian 
Published: October 2023

PurposeThis paper employs nationwide, large-scale field survey data to provide the first empirical evidence on the impact of human educational capital on the acquisition of health entitlement among Chinese migrant workers. The findings of this study hold significant practical implications for the formulation of policies aimed at improving the health protection of migrant workers, as well as for socioeconomic policies during China's transitional period.Design/methodology/approachUsing the IVProbit model, this research examines how human educational capital influences the attainment of health entitlement among migrant workers in China by analyzing the impact and mechanism of education on health entitlement. The study is based on the China Migrants Dynamic Survey (CMDS) data from 2018, which include 100,177 observations.FindingsFor migrant workers in China, higher levels of education have a significant positive effect on the acquisition of health entitlements, including medical insurance, health records and health education. The positive impact of human educational capital on health entitlements is more significant for non-provincial cities and young-generation migrant workers. The results also show that human educational capital can influence the acquisition of health entitlements through mediators such as financial status, social integration and health status.Originality/valueThis study represents the first empirical attempt to evaluate the influence of human educational capital on the access of migrant workers in China to health rights and interests. Additionally, the study develops a theoretical framework to examine how the impact of human educational capital varies across migrant workers with different characteristics and their access to health entitlements.
Big ideas, soft governance: managing large-scale school improvement at the national agency level in Sweden
Authors: Nordholm Daniel , Adolfsson Carl-Henrik 
Published: October 2023

PurposeUsing a large-scale school improvement program in Sweden as a case, this article aims to explore the state governance of a large-scale school improvement program in Sweden and how officials at the state agency level made sense of the reform ideas and operationalized them in policy actions.Design/methodology/approachData were integrated from Swedish Government Official Reports and formal directives from the Ministry of Education. Officials of the Swedish National Agency for Education (SNAE) were also interviewed. Data were analyzed to identify how regulatory rules, professional norms and cultural–cognitive beliefs shaped SNAE's design of the program.FindingsThe article shows how different types of governance (i.e. regulatory rules, professional norms and cultural–cognitive beliefs) set the direction for managing large-scale school improvement. In particular, in the studied case, the lack of clear regulatory directives enabled sensemaking processes clearly influenced by normative ideas and cultural–cognitive beliefs.Research limitations/implicationsThe findings are mostly presented from the perspective of managers, so further study is required to attain a broader understanding of the state agency level's role and function.Practical implicationsBy illustrating the strengths of understanding various dimensions of educational governance, the findings are highly relevant to both policymakers and educational managers at different levels of school systems.Originality/valueThe article offers a valuable perspective on large-scale school improvement and educational governance by focusing on a level that has hitherto received little attention.
Study trips: an essential educational component for the training of agribusiness managers
Authors: Islas-Moreno Asael , Uriza-Ávila Daniel Emigdio , Guzmán-Elizalde Ana Lieseld , Aguirre-Álvarez Gabriel 
Published: October 2023

PurposeThe study aims to analyze the effect of the previous preparation and the work carried out in the field during a study trip on the development of competencies in agribusiness students.Design/methodology/approachThe destination was the pineapple area of the Papaloapan Lower Basin in Mexico, and 42 students from 6 different semester levels participated. The students answered a test prior to the trip, received an evaluation for their activities in the field and prepared reports and posters as products of the experience. The relationship between the scores obtained was examined through a comparative analysis.FindingsThe findings are framed in the cyclical model of experiential learning with four stages (feeling, watching, thinking and doing) by Kolb (1984). It is found that the acquisition of specific knowledge about what the experience will entail leads to better preparation, motivation and confidence to live the experience (potentiation of feeling and watching). In turn, specific knowledge and better use of experience promote the development of problem solving, interpersonal and communication skills (potentiation of thinking and doing).Research limitations/implicationsStatistical representativeness is not a quality of the study since it is based on a comparative analysis.Originality/valueThe study analyzes an educational component of great value in the business area, about which little is known in the agribusiness subarea.
Digital service innovation challenges faced during servitization: a multi-level perspective
Authors: Burton Jamie , Story Victoria Mary , Zolkiewski Judy , Nisha Nazifa 
Published: October 2023

PurposeDigital Service innovation (DSI) plays a fundamental role in the successful transition from product manufacturer or traditional service provider to a provider of digitally-enabled service solutions. Multiple impediments make managing this transformation using digital technologies difficult for firms, their customers and wider ecosystems. Extant knowledge of these digital technology impediments requires synthesizing and mapping.Design/methodology/approachA systematic literature review (SLR) was conducted. DSI tools and terminology are synthesized via thematic analysis. Subsequently, impediments to DSI for servitization (covering barriers, challenges and tensions) faced by actors across three key innovation phases: strategic planning, design planning and implementation, and four interaction levels (Micro, Meso, Macro-environment, Macro-ecosystem) are mapped via template analysis.FindingsSix impediment categories (external environmental factors, internal firm factors, capabilities, business models and processes, value creation and interaction) encompassing 28 unique impediment types to DSI during servitization are identified. A framework enabling impediment comparison across innovation phases and ecosystem/network interaction levels, revealing that the majority of barriers can be framed as “challenges” was developed.Originality/valueWhilst literature is emerging relating to digital servitization, there is a lack of research on the role DSI plays in facilitating digital servitization and no comprehensive study of DSI impediments exists. Additionally, consensus around the cross-disciplinary terminologies used is lacking. This study is a structured attempt to map the domain, summarizing the terms, identifying and clarifying impediment categories and providing recommendations for researchers and managers in tackling the latter.
A Tale of Two Annies: Historical Memory, Archives and the Perpetuation of the Sinners to Angels Trope in American Sex Worker History
Authors: Barnes‐Gilbert Ashley 
Published: October 2023

AbstractAs a historian of sex work, I analyse the power dynamics in the archiving practices and interpretation of sex worker lives, deconstructing the historic and current discourses shaping the possibilities for sex workers. In this article, I explore the legends of nineteenth‐century Madams Annie Cook and Annie Chambers. According to the remaining historical record, Cook and Chambers transformed from sinners to angels prior to death – using sacrifice to eschew their lives of alleged immorality – and in the process, became worthy of historical remembrance and archiving. In recognising the politics of archiving certain documents but not others, I deconstruct the histories of Cook and Chambers to show how discourse and historical memory function to simplify sex worker lives. These tropes serve a larger purpose of constructing white sex worker lives as redeemable and valuable and Black sex workers as unworthy of historical memory, shaping not only the history of sex work but also current debates about sex worker lives, sex trafficking, legal rights and belonging.
Creating a space for trans self‐narrative in 1930s Turkey: Kenan Çinili's memoir
Authors: Sarıtaş Ezgi 
Published: October 2023

AbstractThe memoir of Kenan Çinili, a transgender person who was widely covered by newspapers in Turkey in the second half of the 1930s, sheds light on the historically and geographically unique workings of cisheteronormativity. Through a self‐reflexive reading of the memoir and newspaper accounts from this period, this article explores how a narrative transgender subjectivity emerges from the appropriation and negotiation of tropes, figures and discourses that make this subjectivity intelligible. Through the use of the medium of photography, which exposed and spectacularised gender nonconformity, Kenan was able to performatively embody and visually present a masculine gender identity. However, the space Kenan created for narrative and visual self‐constitution was conditioned by their privileges based on class, urban–rural hierarchy, ethnicity and normative sexual dimorphism.
COVID‐19 vaccine hesitancy in rural and metropolitan Western Australia: A mid‐rollout cross‐sectional analysis of why it exists and potential solutions
Authors:   STRIVE WA Collaborative, Damianopoulos Nicholas , Leigh James , Pugliese Matthew , Frayne Jacqueline , Richards Toby 
Published: October 2023

AbstractIntroductionVaccine hesitancy represents a state of uncertainty before a decision about vaccination is made. It can lead to limited vaccine uptake despite adequate supply and an efficacious product. Western Australia (WA) presents a unique challenge with a population widely spread across metropolitan and rural sites and diverse opinions regarding vaccination.ObjectiveTo elicit and compare the common COVID‐19 vaccine concerns in rural and metropolitan WA, and to identify proposed solutions to vaccine hesitancy.DesignA voluntary online survey was distributed via social media over a 2‐week period in August 2021 during Phase 2A of the National COVID‐19 Vaccine Rollout Strategy. General practitioners and members of the public living in rural and metropolitan WA over the age of 18 were surveyed regarding COVID‐19 vaccine concerns (blood clots; long‐ and short‐term side effects; lack of testing; and other concerns) and suggested methods to address vaccine hesitancy. Data were analysed with a sequential mixed methods and thematic analysis approach.FindingsThere were 468 general population respondents to our survey, of whom 19.0% (n = 89) lived rurally. A majority (52.6% [n = 246]) of general respondents expressed concerns about COVID‐19 vaccine safety. The commonest concerns were long‐ and short‐term side effects, blood clots and inadequate testing. There was a positive correlation between rurality and vaccine concerns; an inverse relationship between rurality and vaccine uptake; and an inverse relationship between vaccine concerns and uptake. Improved media coverage was the commonest solution suggested to address COVID‐19 vaccine hesitancy.DiscussionA significant proportion of respondents had concerns about vaccine safety; concerns were more common in rural respondents. Rural communities may benefit from location‐targeted media campaigns with a focus on breaking down barriers specific to these members of the population. Vaccine access is more challenging, and consistent messaging from trusted sources is of utmost importance to improve uptake.ConclusionCOVID‐19 vaccine hesitancy is more common in rural populations. Targeted media‐based education regarding vaccine safety may improve COVID‐19 vaccine uptake.
Triple targeting of RSK, AKT, and S6K as pivotal downstream effectors of PDPK1 by TAS0612 in B‐cell lymphomas
Authors: Katsuragawa‐Taminishi Yoko , Mizutani Shinsuke , Kawaji‐Kanayama Yuka , Onishi Akio , Okamoto Haruya , Isa Reiko , Mizuhara Kentaro , Muramatsu Ayako , Fujino Takahiro , Tsukamoto Taku , Shimura Yuji , Taniwaki Masafumi , Miyagawa‐Hayashino Aya , Konishi Eiichi , Kuroda Junya 
Published: October 2023

AbstractB‐cell lymphomas (BCLs) are the most common disease entity among hematological malignancies and have various genetically and molecularly distinct subtypes. In this study, we revealed that the blockade of phosphoinositide‐dependent kinase‐1 (PDPK1), the master kinase of AGC kinases, induces a growth inhibition via cell cycle arrest and the induction of apoptosis in all eight BCL‐derived cell lines examined, including those from activated B‐cell‐like diffuse large B‐cell lymphoma (DLBCL), double expressor DLBCL, Burkitt lymphoma, and follicular lymphoma. We also demonstrated that, in these cell lines, RSK2, AKT, and S6K, but not PLK1, SGK, or PKC, are the major downstream therapeutic target molecules of PDPK1 and that RSK2 plays a central role and AKT and S6K play subsidiary functional roles as the downstream effectors of PDPK1 in cell survival and proliferation. Following these results, we confirmed the antilymphoma efficacy of TAS0612, a triple inhibitor for total RSK, including RSK2, AKT, and S6K, not only in these cell lines, regardless of disease subtypes, but also in all 25 patient‐derived B lymphoma cells of various disease subtypes. At the molecular level, TAS0612 caused significant downregulation of MYC and mTOR target genes while inducing the tumor suppressor TP53INP1 protein in these cell lines. These results prove that the simultaneous blockade of RSK2, AKT, and S6K, which are the pivotal downstream substrates of PDPK1, is a novel therapeutic target for the various disease subtypes of BCLs and line up TAS0612 as an attractive candidate agent for BCLs for future clinical development.
Follow‐up care and support to parents of premature children: Multidisciplinary versus regular follow‐up care
Authors: Bouwmeester Daniëlle , Anolda Naber Fabiënne Bertina , Heyman Hiltje , Hoffmann‐Haringsma Angelique , Lens Annick , Brunt Tibor Markus 
Published: October 2023

AbstractBackgroundPreterm care involves clinical measures almost exclusively aimed at keeping the preterm alive and ready for discharge from hospital. Children are then enrolled in clinical follow‐up care after this stressful period, but mental or specialised care for parents and child is often not embedded in the routine of a neonatal hospital ward and the family is then dependent on institutions for mental health or child health clinics, that is, regular care that is regionally scattered. This study aimed to investigate experiences of parents with regular care and compare them with parents visiting a fixed, specialised, multidisciplinary institute, outside the hospital walls, for preterm follow‐up care.MethodsA survey was conducted among 56 parents (regular follow‐up care N = 26; multidisciplinary follow‐up care N = 30) of children born prematurely. The survey consisted out of items like parents' experiences with follow‐up care, like the organisation of care, supportive care, environmental support and experienced stress.ResultsParents receiving multidisciplinary follow‐up care reported higher levels of satisfaction with organisation of care (F = 5.45; p = 0.023) and supportive care (F = 11.69; p = 0.001) than parents receiving regular follow‐up care. Also, it was found that the extent of support from the social environment alleviated stress but only in parents receiving regular follow‐up care (ß = −0.47; p = 0.016).ConclusionsThe current study findings indicate that experience with follow‐up care is valued higher when receiving multidisciplinary care than regular care, and the degree in environmental support alleviates stress in parents receiving regular care.
Role of SIRT1 in sepsis‐induced encephalopathy: Molecular targets for future therapies
Authors: Shehata Alaa H. , Anter Aliaa F. , Ahmed Al‐Shaimaa F. 
Published: October 2023

AbstractSepsis induces neuroinflammation, BBB disruption, cerebral hypoxia, neuronal mitochondrial dysfunction, and cell death causing sepsis‐associated encephalopathy (SAE). These pathological consequences lead to short‐ and long‐term neurobehavioural deficits. Till now there is no specific treatment that directly improves SAE and its associated behavioural impairments. In this review, we discuss the underlying mechanisms of sepsis‐induced brain injury with a focus on the latest progress regarding neuroprotective effects of SIRT1 (silent mating type information regulation‐2 homologue‐1). SIRT1 is an NAD+‐dependent class III protein deacetylase. It is able to modulate multiple downstream signals (including NF‐κB, HMGB, AMPK, PGC1α and FoxO), which are involved in the development of SAE by its deacetylation activity. There are multiple recent studies showing the neuroprotective effects of SIRT1 in neuroinflammation related diseases. The proposed neuroprotective action of SIRT1 is meant to bring a promising therapeutic strategy for managing SAE and ameliorating its related behavioural deficits.
Contemporary changes in phenotypic variation, and the potential consequences for eco‐evolutionary dynamics
Authors: Sanderson Sarah , Bolnick Daniel I. , Kinnison Michael T. , O'Dea Rose E. , Gorné Lucas D. , Hendry Andrew P. , Gotanda Kiyoko M. 
Published: October 2023

AbstractMost studies assessing rates of phenotypic change focus on population mean trait values, whereas a largely overlooked additional component is changes in population trait variation. Theoretically, eco‐evolutionary dynamics mediated by such changes in trait variation could be as important as those mediated by changes in trait means. To date, however, no study has comprehensively summarised how phenotypic variation is changing in contemporary populations. Here, we explore four questions using a large database: How do changes in trait variances compare to changes in trait means? Do different human disturbances have different effects on trait variance? Do different trait types have different effects on changes in trait variance? Do studies that established a genetic basis for trait change show different patterns from those that did not? We find that changes in variation are typically small; yet we also see some very large changes associated with particular disturbances or trait types. We close by interpreting and discussing the implications of our findings in the context of eco‐evolutionary studies.