bluuebunny/crossref_metadata_2025_split huggingface dataset:
A pan-cancer analysis of the role of argininosuccinate synthase 1 in human tumors
Authors: Ding Qiang , Li Ruiqi , Wang Qingming , Yu Li , Zi Fuming 
Published: November 2023

AimThere is accumulating evidence indicating that ASS1 is closely related to tumors. No pan-cancer analysis of ASS1 was available.MethodsHere we explored the gene expression and survival analysis of ASS1 across thirty-three tumors based on the datasets of the TCGA (Cancer Genome Atlas), the GEO (Gene Expression Omnibus), and the GEPIA2 (Gene Expression Profiling Interactive Analysis, version 2).ResultsASS1 is highly expressed in most normal tissues and is related to the progression of some tumors. We also report ASS1 genetic alteration and their association with tumor prognosis and report differences in ASS1 phosphorylation sites between tumors and control normal tissues. ASS1 expression was associated with the infiltration of cancer-associated fibroblasts (CAFs) for the TCGA tumors of BRCA (Breast invasive carcinoma), CESC (Cervical squamous cell carcinoma and endocervical adenocarcinoma), COAD (Colon adenocarcinoma), ESCA (Esophageal carcinoma), SKCM (Skin cutaneous melanoma), SKCM-Metastasis, TGCT (Testicular germ cell tumors), and endothelial cell for the tumors of BRCA, BRCA-Basal, CESC, ESCA, KIRC (Kidney renal clear cell carcinoma), LUAD (Lung adenocarcinoma), LUSC (Lung squamous cell carcinoma), SKCM, SKCM-Metastasis, SKCM-Primary, STAD (Stomach adenocarcinoma), and TGCT. The KEGG and GO analysis were used to analyze ASS1-related signaling pathways. Finally, we used Huh7 cell line to verify the function of ASS1 in vitro. After ASS1 knockdown using small interfering RNA (siRNA), the proliferation and invasion of Huh7 were enhanced, cyclin D1 was up-regulated, and anti-apoptotic protein bax was down-regulated, suggesting that ASS1 is a tumor suppressor gene in hepatocellular carcinoma.ConclusionOur first pan-cancer study offers a relatively comprehensive understanding of the roles of ASS1 in different tumors.
GPR15 in colon cancer development and anti-tumor immune responses
Authors: Namkoong Hong , Lee Bomi , Swaminathan Gayathri , Koh Seong-Joon , Rogalla Stephan , Paraskevopoulou Maria D. , Tang Jay , Mikhail David , Becker Laren S. , Habtezion Aida 
Published: November 2023

IntroductionThe chemoattractant receptor, G protein-coupled receptor 15 (GPR15), promotes colon homing of T cells in health and colitis. GPR15 function in colon cancer is largely unexplored, motivating our current studies.MethodsIn human study, immune cells were isolated from tumor tissues and healthy surgical tumor margins (STM), and their proportions as well as expression of GPR15 was analyzed by flow cytometry. In mouse studies, colon cancer was induced in GPR15-deficient (KO) and GPR15-suficient (Het) mice using azoxymethane (AOM) and dextran sulfate sodium (DSS) solution in drinking water. Serial endoscopy was performed in mice to monitor and visualize the distal region of colon. Mice were euthanized 10 weeks after the initial DSS administration, and the colon length and the number of polyps were recorded. Next, we identified the effects of GPR15L on established tumors in the MC38-colorectal cancer (CRC) mouse model. Immune cells were isolated from the mice colons or tumors and assessed by flow cytometry.ResultsOur analysis of human CRC tissue revealed a significant reduction in GPR15+ immune cell frequencies in tumors compared to ‘tumor-free’ surgical margins. Similarly, our data analysis using The Cancer Genome Atlas (TCGA) indicated that lower GPR15 expression is associated with poor survival in human colon cancer. In the AOM/DSS colitis-associated colon cancer model, we observed increased colonic polyps and lower survival in Gpr15+-KO compared to Gpr15-Het mice. Analysis of immune cell infiltrates in the colonic polyps showed significantly decreased CD8+ T cells and increased IL-17+ CD4+ and IL-17+ CD8+ T cells in Gpr15-KO than in Het mice. Consistent with a protective role of GPR15, administration of GPR15L to established tumors in the MC38-CRC model increased CD45+ cell infiltration, enhanced TNFa expression on CD4+ and CD8+ T cells at the tumor site and dramatically reduced tumor burden.DiscussionOur findings highlight an important, unidentified role of the GPR15-GPR15L axis in promoting a tumor-suppressive immune microenvironment and unveils a novel, colon-specific therapeutic target for CRC.
Lysolipids are prominent in subretinal drusenoid deposits, a high-risk phenotype in age-related macular degeneration
Authors: Anderson David M. G. , Kotnala Ankita , Migas Lukasz G. , Patterson N. Heath , Tideman Léonore E. M. , Cao Dongfeng , Adhikari Bibek , Messinger Jeffrey D. , Ach Thomas , Tortorella Sara , Van de Plas Raf , Curcio Christine A. , Schey Kevin L. 
Published: November 2023

IntroductionAge related macular degeneration (AMD) causes legal blindness worldwide, with few therapeutic targets in early disease and no treatments for 80% of cases. Extracellular deposits, including drusen and subretinal drusenoid deposits (SDD; also called reticular pseudodrusen), disrupt cone and rod photoreceptor functions and strongly confer risk for advanced disease. Due to the differential cholesterol composition of drusen and SDD, lipid transfer and cycling between photoreceptors and support cells are candidate dysregulated pathways leading to deposit formation. The current study explores this hypothesis through a comprehensive lipid compositional analysis of SDD.MethodsHistology and transmission electron microscopy were used to characterize the morphology of SDD. Highly sensitive tools of imaging mass spectrometry (IMS) and nano liquid chromatography tandem mass spectrometry (nLC-MS/MS) in positive and negative ion modes were used to spatially map and identify SDD lipids, respectively. An interpretable supervised machine learning approach was utilized to compare the lipid composition of SDD to regions of uninvolved retina across 1873 IMS features and to automatically discern candidate markers for SDD. Immunohistochemistry (IHC) was used to localize secretory phospholipase A2 group 5 (PLA2G5). ResultsAmong the 1873 detected features in IMS data, three lipid classes, including lysophosphatidylcholine (LysoPC), lysophosphatidylethanolamine (LysoPE) and lysophosphatidic acid (LysoPA) were observed nearly exclusively in SDD while presumed precursors, including phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidic acid (PA) lipids were detected in SDD and adjacent photoreceptor outer segments. Molecular signals specific to SDD were found in central retina and elsewhere. IHC results indicated abundant PLA2G5 in photoreceptors and retinal pigment epithelium (RPE). DiscussionThe abundance of lysolipids in SDD implicates lipid remodeling or degradation in deposit formation, consistent with ultrastructural evidence of electron dense lipid-containing structures distinct from photoreceptor outer segment disks and immunolocalization of secretory PLA2G5 in photoreceptors and RPE. Further studies are required to understand the role of lipid signals observed in and around SDD.
Detection of volatile organic compounds in headspace of Klebsiella pneumoniae and Klebsiella oxytoca colonies
Authors: Bous Michelle , Tielsch Malte , Papan Cihan , Kaiser Elisabeth , Weber Regine , Baumbach Jörg Ingo , Becker Sören L. , Zemlin Michael , Goedicke-Fritz Sybelle 
Published: November 2023

IntroductionEarly diagnosis of infections and sepsis is essential as adequate therapy improves the outcome. Unfortunately, current diagnostics are invasive and time-consuming, making diagnosis difficult, especially in neonatology. Novel non-invasive analytical methods might be suitable to detect an infection at an early stage and might even allow identification of the pathogen. Our aim is to identify specific profiles of volatile organic compounds (VOCs) of bacterial species.MethodsUsing multicapillary column-coupled ion mobility spectrometry (MCC/IMS), we performed headspace measurements of bacterial cultures from skin and anal swabs of premature infants obtained during weekly screening for bacterial colonization according to KRINKO. We analyzed 25 Klebsiella pneumoniae (KP) cultures on MacConkey (MC) agar plates, 25 Klebsiella oxytoca (KO) cultures on MC agar and 25 bare MC agar plates as a control group.ResultsUsing MCC/IMS, we identified a total of 159 VOC peaks. 85 peaks allowed discriminating KP and bare MC agar plates, and 51 peaks comparing KO and bare MC agar plates and 6 peaks between KP and KO (significance level of p &lt; 0.05 after Bonferroni post hoc analysis), respectively. Peaks P51 (n-Decane) and P158 (Phenylethyl Alcohol), showed the best sensitivity/specificity/ positive predictive value/negative predictive value of 99.9% each (p &lt; 0.001) for KP. P158 showed the best sensitivity/specificity/positive predictive value/negative predictive value of 99.9% each (p &lt; 0.001) for KO. Comparing KP and KO, best differentiation was enabled using peaks P72, P97 and P16 with sensitivity/specificity/positive predictive value/negative predictive value of 76.0%, 84.0%, 82.6%, 77.8%, respectively (p &lt; 0.05).DiscussionWe developed a method for the analysis of VOC profiles of bacteria. Using MCC/IMS, we demonstrated that VOCs derived from bacteria are clearly distinguishable from a bare agar plate. Characteristic peaks obtained by MCC/IMS are particularly suitable for the species-specific identification and differentiation of KP and KO. Thus, MCC/IMS might be a useful tool for in vitro diagnostics. Future studies must clarify whether similar patterns of VOCs can be detected in vivo in patients that are colonized or infected with KP or KO to enable rapid and accurate diagnosis of bacterial colonization.
From juniors to seniors: changes in training characteristics and aerobic power in 17 world-class cross-country skiers
Authors: Walther Jacob , Haugen Thomas , Solli Guro Strøm , Tønnessen Espen , Sandbakk Øyvind 
Published: November 2023

Purpose: To compare training characteristics and aerobic power (VO2max) between the most successful junior and senior seasons of world-class cross-country (XC) skiers and to identify differences between sexes and among sprint and distance skiers.Methods: Retrospective analysis was conducted on self-reported training and VO2max tests of ten male and seven female world-class XC-skiers, collectively holding 38 Olympic medals. Training was categorized by form (endurance, strength, speed, other) and mode (specific, unspecific) and was divided into low- (LIT), moderate- (MIT), and high-intensity training (HIT).Results: Total training increased by 203 ± 130 h (35% ± 31%, p &lt; .001, large effect) and 78 ± 69 sessions (21% ± 24%, p &lt; .001, very large effect). Junior training volume (658 ± 107 h) did not correlate with senior volume (861 ± 74 h) but correlated negatively with changes in volume (r = −.822, p &lt; .001). No sex differences were observed related to total volume, but distance skiers increased their total volume more than sprint skiers (p = .037, large effect). Endurance training increased by 197 ± 117 h (p &lt; .001; large effect) tied to increased low-intensity training (186 ± 115 h, p &lt; .001; large effect) and moderate-intensity training (13 ± 7 h, p &lt; .001; large effect). Training intensity distribution (% LIT/MIT/HIT) was 91/3/6 in junior and 92/4/4 in senior season. Women demonstrated greater increase of unspecific modes (100 ± 58 vs. 37 ± 44 h, p = .022; large effect) and strength training (25 ± 23 vs. −3 ± 17 h, p = .010, large effect). Men improved absolute (8% ± 5%; p = .009; large effect) and relative VO2max (6% ± 4%; p = .016; large effect) from junior to senior, while women only increased relative VO2max (7% ± 5%, p = .012; large effect).Conclusion: This study provides novel information regarding changes in training characteristics and aerobic power from junior to senior age in world-class XC-skiers. Overall, the enhanced training volume during this transition was primarily driven by increased LIT and MIT and the exceptionally high relative VO2max at junior age further increased in both sexes.
Promising physiological traits associated with nitrogen use efficiency in rice under reduced N application
Authors: Srikanth Bathula , Subrahmanyam Desiraju , Sanjeeva Rao Durbha , Narender Reddy Sadu , Supriya Kallakuri , Raghuveer Rao Puskur , Surekha Kuchi , Sundaram Raman Meenakshi , Neeraja Chirravuri Naga 
Published: November 2023

Higher grain yield in high-yielding rice varieties is mostly driven by nitrogen (N) fertilizer applied in abundant amounts leading to increased production cost and environmental pollution. This has fueled the studies on nitrogen use efficiency (NUE) to decrease the N fertilizer application in rice to the possible extent. NUE is a complex physiological trait controlled by multiple genes, but yet to be completely deciphered in rice. With an objective of identifying the promising physiological traits associated with NUE in rice, the performance of 14 rice genotypes was assessed at N0, N50, N100, and N150 for four (two wet and two dry) seasons using agro-morphological, grain yield, flag leaf traits, photosynthetic pigment content, flag leaf gas exchange traits, and chlorophyll fluorescence traits. Furthermore, the data were used to derive various NUE indices to identify the most appropriate indices useful to screen rice genotypes at N50. Results indicate that with the increase in N application, cumulative grain yield increased significantly up to N100 (5.02 t ha−1); however, the increment in grain yield was marginal at N150 (5.09 t ha−1). The mean reduction of grain yield was only 26.66% at N50 ranging from 15.0% to 34.2%. The significant finding of the study is the identification of flag leaf chlorophyll fluorescence traits (Fv/Fm, ΦPSII, ETR, and qP) and Ci associated with grain yield under N50, which can be used to screen N use efficient genotypes in rice under reduced N application. Out of nine NUE indices assessed, NUpE, NUtE, and NUEyield were able to delineate the high-yielding genotypes at N50 and were useful to screen rice under reduced N conditions. Birupa emerged as one of the high yielders under N50, even though it is a moderate yielder at N100 and infers the possibility of cultivating some of the released rice varieties under reduced N inputs. The study indicates the possibility of the existence of promising genetic variability for grain yield under reduced N, the potential of flag leaf chlorophyll fluorescence, and gas exchange traits as physiological markers and best suitable NUE indices to be deployed in rice breeding programs.
Autobiographical memory in contact tracing: evidence from the COVID-19 pandemic
Authors: Rossi-Arnaud Clelia , Mastroberardino Serena , Spataro Pietro , Santirocchi Alessandro , Alessi Federica , Naser Aicia , Pesola Maria Chiara , Cestari Vincenzo 
Published: November 2023

IntroductionThe recent COVID-19 pandemic has compelled various governments to trace all contacts of a confirmed case, as well as to identify the locations visited by infected individuals. This task, that requires the activation of our autobiographical memories, can make a difference in the spread of the contagion and was based primarily on telephone interviews with infected people. In this study, we examined whether participants were able to provide contact tracing information and whether their memories were influenced by salient events occurring during the initial phases of the pandemic.MethodsParticipants were asked to fill in an online standardized form in which they recounted every day of the 2 weeks before, reporting as much information as possible. The time period selected included, among other things, the day on which the Italian government issued the decree initiating the COVID-19 lockdown. The task was completed twice, the first time relying solely on their memory, and the second time using external aids (diaries, mobile phones etc.). Reports were then coded using a scheme that segmented accounts into informational details, divided into two broad categories, internal and external.ResultsOur findings showed that (i) the use of external aids was effective only when participants had to recall the day furthest away or if to-be-recalled events have low distinctiveness, and (ii) memories of internal details were recalled better than memories of external details. Participants were overall accurate and reported a large amount of information about people and places. However, because of the connection with key pandemic-related events, the effect was somewhat stronger on specific days (e.g., the day in which the lockdown was announced).DiscussionThe results of this work could provide a useful tool for improving the design of contact tracing procedures in the event of an unwanted future public health crisis caused by a highly infectious agent.
A model linking digital media dependence, exercise empowerment, and social physique anxiety among emerging adulthood college students
Authors: Luo Lin , Yuan Junfeng , Bi Siyuan , Wang Yanlin 
Published: November 2023

Background/objectiveSocial physique anxiety (SPA) is a prevalent psychological issue among emerging adults, regardless of gender. Many studies have shown that high levels of SPA are associated with various negative consequences on both physical and mental well-being. Considering the potential severity of SPA’s consequences and its high prevalence among emerging adults, it is imperative to investigate the factors and mechanisms that contribute to SPA in this population. Although prior studies have identified associations between emerging adulthood, digital media use, and SPA in young individuals, the underlying mechanisms remain unclear. The objective of this study is to examine the associations between SPA, emerging adulthood characteristics, digital media dependency, and exercise empowerment.MethodsIn this cross-sectional study, Chinese college students were recruited using snowball sampling. The study utilized an online survey to assess SPA, emerging adulthood characteristics, digital media dependency, and exercise empowerment. The collected data was analyzed using path analysis.ResultsA total of 1,661 Chinese college students (mean age = 19.63 ± 0.32 years, 44.97% male) were included in this study. The results showed that SPA exhibited positive correlations with responsibility and instability in emerging adulthood characteristics, digital media dependency, and exercise empowerment. Additionally, digital media dependency showed positive correlations with responsibility and instability, as well as with exercise empowerment. Furthermore, exercise empowerment demonstrated positive correlations with self-exploration, responsibility, instability, and possibilities in emerging adulthood characteristics. SPA can be directly influenced by digital media dependency, self-exploration, and instability. Furthermore, digital media dependency has a positive indirect impact on SPA through exercise empowerment. Similarly, self-exploration also has a positive indirect impact on SPA through exercise empowerment. On the other hand, instability has a negative indirect impact on SPA through exercise empowerment.ConclusionThis study provides new insights into the complex correlations with emerging adulthood characteristics, digital media dependency, exercise empowerment, and SPA. Instability, self-exploration in emerging adulthood characteristics, as well as digital media dependency, have the potential to influence SPA among college students through exercise empowerment Interventions and strategies aimed at addressing these psychological factors may prove beneficial in reducing SPA among emerging adults, especially college students.
Assessing empathy in healthcare services: a systematic review of South American healthcare workers’ and patients’ perceptions
Authors: Moya-Salazar Jeel , Goicochea-Palomino Eliane A , Porras-Guillermo Jorge , Cañari Betsy , Jaime-Quispe Alexis , Zuñiga Nahomi , Moya-Salazar María Jesús , Contreras-Pulache Hans 
Published: November 2023

BackgroundEmpathy in healthcare service refers to the ability of healthcare workers (HCWs) to put themselves in patients’ shoes, which is necessary to ensure a good physician-patient relationship and provide quality care. Various studies have shown that empathy varies depending on the country, the instrument used, the evaluator, and the HCW’s specialty. This systematic review aims to estimate the levels of empathy among HCWs in South American countries between 2000 and 2019.MethodsWe conducted searches in 15 databases (PubMed, Scopus, Web of Science, EMBASE, Scielo, PsycoInfo, ScientDirect, Latindex, and LILIACS), four preprint servers (medRxiv, bioRxiv, SportRxiv, and Preprints), and other search engines such as Dimensions (20), Google Scholar, Yahoo!, and Alicia CONCyTec (c). We followed the PRISMA guidelines, and this study was registered in PROSPERO (CRD42023454007).ResultsOut of 18,532 documents identified from November 10 to 28, 2021, 10 articles were included (n = 2,487 participants, of which 1989 were patients). Among the studies focusing on self-evaluated empathy, four relied on the Jefferson Scale of Empathy for medical professionals (JSE-HP). However, assessments from patients employing Jefferson Scale of Patient’s Perceptions of Physician Empathy (JSPPPE) and Consultation and Relational Empathy (CARE) scale suggested high levels of empathy We found that both professionals and patients perceived that empathic care was provided, often at a medium or regular level. Surgery residents presented lower levels of empathy compared to obstetrics-gynecology and pediatrics physicians.ConclusionEmpathy is crucial in determining the quality of care and patient satisfaction during healthcare services provided by HCWs. Therefore, it is important to support professionals so that the various stressful situations they encounter in their work and daily life do not negatively influence the approach they provide to patients.
From the dual-dimensional perspective of employee mindfulness and superior trust, explore the influence mechanism of negative workplace gossip on work engagement
Authors: Cheng Xiaoli , Duan Jiaxin , Wu Weilin , Lu Lei 
Published: November 2023

IntroductionAs a common phenomenon of workplace negative gossip in organizations, how it affects employees’ work engagement is not yet clear, nor what methods can be used to mitigate its negative impact on employees’ work engagement.MethodsBased on Conservation of Resource Theory, this study obtained 334 valid employee samples from mainland China enterprises through a three-time lagged research design and explored the mechanism of negative workplace gossip on work engagement from the dual perspectives of employees and supervisors.ResultsThe results show that: (1) Negative workplace gossip negatively affects employee work engagement. (2) Professional commitment plays a mediating role between negative workplace gossip and employee work engagement. (3) Employee mindfulness negatively moderates the negative impact of workplace negative gossip on professional commitment; superior trust negatively moderates the negative impact of workplace negative gossip on professional commitment. (4) Employee mindfulness and superior trust are further weakened to moderate the negative indirect impact of workplace negative gossip on employee work engagement through professional commitment, and this negative indirect impact is weaker when employees have a higher degree of mindfulness and higher trust in superiors.DiscussionIt proposes effective strategies for managing workplace gossip to harness its positive influence and offer practical guidance to enhance employee work engagement.