bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alpha-synuclein overexpression can drive microbiome dysbiosis in mice
Authors: Sampson Timothy R. , Wallen Zachary D , Won Woong-Jai , Standaert David G. , Payami Haydeh , Harms Ashley S. 
Published: February 2024

ABSTRACTGrowing evidence indicates that persons living with Parkinson disease (PD), have a unique composition of indigenous gut microbes. Given the long prodromal or pre-diagnosed period, longitudinal studies of the human and rodent gut microbiome prior to symptomatic onset and for the duration of the disease period are currently lacking. PD is characterized in part by accumulation of the protein α-synuclein (α-syn) into insoluble aggregates, in both the central and enteric nervous systems. As such, a number of experimental rodent and non-human primate models of α-syn overexpression recapitulate some of hallmark pathophysiologies of PD. These animal models provide an opportunity to assess how the gut microbiome changes with age under disease relevant conditions. Here, we used a transgenic mouse strain, the Thy1-hSYN “line 61” mice which over express wild-type human α-syn to test how the gut microbiome composition responds in this model of PD pathology during aging. Using shotgun metagenomics, we find significant, age and genotype dependent bacterial taxa that become altered over age. We reveal that α-syn overexpression can drive alterations to the gut microbiome composition and suggest that it limits the expansion of diversity through age. Given emerging data on potential contributions of the gut microbiome to PD pathologies, our data provide an experimental foundation to understand how the PD-associated microbiome may arise as a trigger or co-pathology to disease.
The Impact of Mismatches Within the RNA: DNA Hybrid in the Transposon-Encoded Type I-F CRISPR-Cas System
Authors: Alalmaie Amnah , Khashan Raed 
Published: February 2024

AbstractRecent research has revealed a collaboration between type I-F CRISPR systems and Tn7 transposons in certain bacteria, leading to the discovery of a new gene-editing tool called INTEGRATE. This system integrates transposons into the target strand without introducing a double-strand break or repair mechanism, making it highly promising. The published results showed that a full match between the spacer region of crRNA and the target DNA is necessary to form a stable and complete R-loop, which is critical for accurate integration. PAM-distal mismatches affected RNA-guided DNA transposition differently, with mismatches in positions 25-28 completely blocking the process, while mismatches in positions 29-32 were tolerated. To understand the impact of PAM-distal mismatches on the R-loop stabilization and transposition process, classical all-atom molecular dynamics simulations were conducted using three independent models, including one with a complete RNA-DNA match and two with PAM-distal mismatches located at positions 25-32. Our results suggest that the stable rotation of Cas8-HB is a critical step in the interaction with TniQ and initiation of the DNA transposition process. Network analysis techniques were employed to investigate the communication pathways within Cas8 and TniQ dimers, including eigenvector centrality and correlation analysis. These techniques revealed that certain amino acids in TniQ and Cas8 were highly central to the communication pathways, as they exhibited significant changes in centrality under both mismatches. The findings discussed in this study provide valuable insights into the mechanisms involved in the DNA transposition process and shed light on how PAM-distal mismatches can affect these mechanisms.
Microbial remineralization processes during post-spring-bloom excess phosphate in the northern Baltic Sea
Authors: Vanharanta Mari , Santoro Mariano , Villena-Alemany Cristian , Piiparinen Jonna , Piwosz Kasia , Grossart Hans-Peter , Labrenz Matthias , Spilling Kristian 
Published: February 2024

AbstractIn the northern Baltic, post-spring-bloom low dissolved inorganic nitrogen to phosphorus conditions, degradation of N-rich organic matter potentially supports the drawdown of excess phosphate. During a 17-day-long mesocosm experiment in the south-west Finnish archipelago, we examined nitrogen, phosphorus and carbon acquiring extracellular enzyme activities in three size fractions (<0.2 µm, 0.2–3 µm, and >3 µm), bacterial abundance, production, community composition and its predicted metabolic functions. The mesocosms received different carbon and nitrogen amendments to test for the effect of inorganic nutrient stoichiometry on enzymatic degradation processes that ultimately determine the export potential of organic matter. Alkaline phosphatase activity occurred mainly in the dissolved form and likely contributed to the excess phosphate conditions. In the beginning of the experiment, peptidolytic and glycolytic enzymes were predicted to be produced by free-living bacteria identified within the classes Actinobacteria and Alphaproteobacteria, whereas the contribution of picocyanobacteria increased towards the end. Our results imply that heterotrophic bacteria lost the competition to picocyanobacteria due to the lack of suitable energy sources. The high hydrolytic rates in fractions <0.2 µm and 0.2–3 µm, found in this study, could potentially retain inorganic nutrients in the surface layer and suppress downward fluxes of organic matter and hence carbon sequestration.
Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons
Authors: Sichlinger Laura , Wulf Maximilian , Dutan Polit Lucia , Nasser Fatema , Duarte Rodrigo R.R. , Powell Timothy R. , Marcus Katrin , Vernon Anthony C. , Srivastava Deepak P. 
Published: February 2024

AbstractZNF804Awas amongst the first genes robustly associated with schizophrenia based on findings from large-scale genomic studies. Previous research has implicatedZNF804Ain the regulation of gene expression and synaptic function, but the role of this gene in neurodevelopment and in schizophrenia pathogenesis remains unclear. To study its function during neurodevelopment, we generated isogenic human induced pluripotent stem cells (hiPSCs) with reducedZNF804Aexpression, differentiated them into developing cortical glutamatergic neurons and studied their transcriptomic, synaptic and protein signatures. Mutant neurons showed modest evidence changes in gene expression. However, high-content confocal imaging revealed increased excitatory synapse density in mutant neurons. Cell-compartment specific proteomic analysis further revealed that mutant neurons had higher levels of ribosomal and translational proteins within neurites, and high-content imaging confirmed increased local protein synthesis efficiency. Overall, these results demonstrate that in human developing cortical glutamatergic neurons,ZNF804Aregulates excitatory synapse formation potential via increased local protein translation.
Accessing the subleading-twist
B
-meson light-cone distribution amplitude with large-momentum effective theory
Authors: Hu Shu-Man , Wang Wei , Xu Ji , Zhao Shuai 
Published: February 2024

Within the framework of large-momentum effective theory (LaMET), we propose a hard-collinear factorization formula to extract the subleading-twist B-meson light-cone distribution amplitude (LCDA) from the quasidistribution amplitude calculated on the lattice. The one-loop matching coefficient and an integro-differential equation governing the evolution of the quasidistribution amplitude are derived. Our results could be useful either in evaluating the subleading-twist B-meson LCDA on the lattice or in the understanding the feasibility of LaMET on higher-twist distributions.

Published by the American Physical Society
2024
Mobile banking app experience of generation Y and Z consumers
Authors: Sharma Neeru , Sharma Meena , Singh Tejinderpal 
Published: February 2024

PurposeThe study investigates whether the customer experience, satisfaction and continuance intention interrelationships in mobile banking services vary across Generation (Gen) Y and Gen Z consumers.Design/methodology/approachThe data were collected using online surveys from 224 Gen Y and 238 Gen Z mobile banking users. The study uses the partial least squares structural equation modelling (PLS-SEM) technique and an asymmetrical analytical approach through fuzzy set qualitative comparative analysis (fsQCA) to examine the effects of five experience dimensions (pragmatic, usability, affective, sensory and social) and satisfaction on continuance intention.FindingsWhilst Gen Z values pragmatic and affective experiences more than Gen Y and assigns less importance to usability experience (Uxp), both PLS-SEM and fsQCA did not find any significant impact of sensory experience (Sxp) in any cohort. In contrast, fsQCA suggests that social experience could play a significant role for specific segments within both generations. Furthermore, PLS-SEM demonstrates a greater impact of satisfaction on continuance intention for Gen Y than for Gen Z.Research limitations/implicationsThe sample consists of high/middle-income urban consumers in one country. Future research could investigate low-income and semi-urban/rural consumers and consumers living in other countries.Practical implicationsBanks must recognise the diversity within and between Gen Y and Gen Z, adopting a segmented user experience approach. Users within each generation may prioritise distinct aspects of the mobile banking app and understanding the specific differences between Gen Y and Gen Z preferences is crucial.Social implicationsEncouraging mobile banking users to engage in community-driven financial initiatives can inspire non-users, promoting digital financial inclusion.Originality/valueTo the best of the authors' knowledge, this study is the first to compare the customer experience-based psychological patterns of continued mobile banking use in Gen Y and Gen Z.
Lean vs agile project management in construction: impacts on project performance outcomes
Authors: Badran Sara S. , Abdallah Ayman Bahjat 
Published: February 2024

PurposeThe present research aims to investigate how lean project management (LPM) and agile project management (APM) affect project performance outcomes in the construction sector in Jordan. This study focuses on six key project performance outcomes, namely cost, time, quality, client satisfaction, innovation and responsiveness.Design/methodology/approachThe present study employed a quantitative approach to achieve the research objectives. Accordingly, a multi-item survey questionnaire was prepared to gather data from 392 project managers from construction companies in Jordan. The study’s model showed acceptable levels regarding reliability, validity, fit indices and discriminant validity. In order to test the hypotheses of this study, path analysis was employed using Amos 24.0 software.FindingsLPM demonstrated a remarkably high positive impact on cost performance. It also positively affected quality performance and client satisfaction. However, LPM insignificantly affected time, innovation and responsiveness performance measures. On the other hand, APM showed a notably high positive impact on innovation and responsiveness. The findings also revealed that APM positively impacted quality performance and client satisfaction. In addition, APM negatively impacted cost performance and insignificantly impacted time performance.Originality/valueThis study is one of the first comprehensive studies to empirically examine the impact of both LPM and APM on various project performance outcomes in the construction industry in the context of a developing country. It reveals some similarities and differences between LPM and APM with regard to their impacts on project management outcomes. The findings are expected to guide managers in selecting the appropriate project management approach based on the desired performance outcomes. Accordingly, it offers important implications for project managers in construction companies.
Gender-based policies and women’s entrepreneurship: an fsQCA analysis of sub-Saharan African countries
Authors: Adegbile Abiodun Samuel , Ogundana Oyedele Martins , Adesola Sola 
Published: February 2024

PurposeEntrepreneurship policy is a vital component of any entrepreneurial ecosystem. However, the specific policy initiatives that have a greater impact on women's entrepreneurship remain unclear in many developing economies. Therefore, this study aims to evaluate the effectiveness of entrepreneurship policies targeted at women’s entrepreneurship in sub-Saharan Africa (SSA).Design/methodology/approachEmploying fuzzy-set qualitative comparative analysis (fsQCA), this paper utilises and analyses secondary data collected by the World Bank's Women, Business, and the Law (WBL) from 1970 to 2020, encompassing 48 countries within SSA.FindingsThrough our analysis, we identified two configurations that sufficiently support women's entrepreneurship. First, a combination of gender-based policies focussing on enabling “access to credit” and “signing of contracts”; and second, a blend of policies supporting “signing of contracts”, “business registration”, and “opening a bank account”, represent significant antecedents to supporting women's entrepreneurship. These distinct pathways are crucial to fostering women’s entrepreneurship in the SSA region.Research limitations/implicationsThe study's findings indicate that the impact and effectiveness of entrepreneurship policies targeted at women entrepreneurs in developing economies depend on the effectiveness of other policies that are in place.Originality/valueThis study offers new insights into the intricate interrelationship between entrepreneurship policies and women’s entrepreneurship in developing countries by considering the interdependence and combinative value of gender-based policies that effectively support women’s entrepreneurship in sub-Saharan Africa.
The authorship of teachers: jissen kiroku as the core of professionalism in Japanese jugyo kenkyu
Authors: Miyamoto Yuichi 
Published: February 2024

PurposeThis paper aims to discuss the significance of teacher authorship (jissen kiroku) developed during jugyo kenkyu. Specifically, it explores the structural conditions of jugyo kenkyu that enabled the flourishing of jissen kiroku.Design/methodology/approachTo find how jissen kiroku developed in jugyo kenkyu, this paper settled triad of authors-text-readers as the analytical perspective. Disputes through 1960s–1980s are adequate to inquire because it can elucidate how readers read jissen kiroku, which is typically challenging to observe.FindingsJissen kiroku is a powerful tool for semantically preserving, reconstructing and consolidating professional values and knowledge in jugyo kenkyu with deepening connoisseurship. Voluntary educational research associations (VERAs) encourage teachers to write and read jissen kiroku to develop their professionalism, which also helped develop exclusive semantics within the field. These developments were possible due to the public nature of jissen kiroku, disseminated to lesson study (LS) actors, thereby strengthening discussions both inside and outside VERAs.Research limitations/implicationsThe paper proposes shift in views on educational science and emphasizes authorship as authority in that professionalism of teaching can be protected and elevated through authoring.Originality/valueThe significant roles of writing practice have not been explored enough. This paper finds the value of authorship in terms of public nature and openness to all teachers which enable the enhancement of professionalism of the LS field.
Small retail businesses' social media adoption amid a crisis
Authors: Woo Hongjoo , Kwon Wi-Suk , Sadachar Amrut , Tong Zhenghao , Yang Jimin 
Published: February 2024

PurposeWhen retail businesses, especially small businesses with greater vulnerability, could not meet consumers in person during the recent pandemic crisis, how did they adapt to the situation? This study examined how small business practitioners (SBPs’) perceptions, trust and adoption intention levels for social media, as well as the relationships among these variables, changed before and during the crisis based on the integration of the contingency theory and the diffusion of innovation theory (DIT).Design/methodology/approachOnline surveys were conducted with USA SBPs before (n = 175) and during (n = 225) the recent pandemic. The hypotheses were tested using structural equation modeling (SEM), multivariate analysis of variance (MANOVA) and multiple-group SEM analysis.FindingsThe results confirmed significant sequential positive relationships between SBPs’ perceived external pressure and perceived benefits of adopting social media, which in turn led to their trust in and then adoption intentions for social media. Further, the comparisons between the pre- and in-pandemic samples revealed that SBPs’ perceptions and adoption intentions all became significantly higher during (vs before) the pandemic, but the structural relationships among these variables weakened during the pandemic.Originality/valueThis study uses a novel approach to integrate the contingency theory with the DIT to propose small businesses' perceptions, trust and adoption intentions for social media during the innovation decision process under rapid contingency changes. Our findings also offer practical implications including recommendations for small businesses’ innovation management as well as training programs.
Research on cross-city emergency management collaboration mechanism in major emergencies: insights from a knowledge management perspective
Authors: Zhang Lin , Wang Jinyu , Wang Xin , Gao Yingju 
Published: February 2024

Purpose
Based on the perspective of knowledge management, this study aims to discuss how to build cross-city emergency management collaboration mechanism in major emergencies and explore the important role of knowledge management in emergency management collaboration.

Design/methodology/approach
Based on the theoretical analysis of knowledge management and the typical case study of cross-city emergency management collaborative rescue, this study provides an in-depth analysis of how these cities achieve high emergency management performance through multidimensional and multilevel knowledge collaboration, thus revealing the mechanism of knowledge transfer, integration and sharing in achieving high emergency management performance.

Findings
Through analyzing typical cases, this study finds that building a smooth mechanism for multichannel emergency rescue information can promote the diversification of knowledge transfer methods, building a platform-based integration mechanism for emergency rescue information can enhance knowledge integration capabilities and building a linkage mechanism for emergency rescue materials between cities can promote knowledge-sharing level, thereby improving emergency management performance level.

Research limitations/implications
This study has great significance for how to build cross-city emergency management collaboration mechanism in the digital era. In the future, the authors need to further discuss the following two aspects in depth: research on the impact of cross-city emergency management collaboration mechanism on improving the knowledge management capabilities of government emergency management departments; and research on the impact mechanism of knowledge management capabilities on city resilience.

Originality/value
Through case analysis of cross-city emergency management collaborative rescue for major emergencies in China in recent years, this study proposes three specific strategies for cross-city emergency management (smooth, integration and linkage mechanisms) and reveals that these three strategies are essentially aimed at improving the government’s knowledge management level.
Storytelling for understanding: a case study of an English-language digital storytelling service-learning subject for refugee children in Hong Kong
Authors: Shi Huiwen , Cheung Lok Ming Eric 
Published: February 2024

Purpose
While most language departments of the university offer service-learning (SL) subjects based on language teaching, such as “Teaching Chinese as a Second Language in Local Schools” and “Serving the Community through Teaching English,” this paper aims to argue that teaching students to teach language(s) is yet to be the best strategy to serve the service recipients.

Design/methodology/approach
SL is widely understood as an experiential learning pedagogy that integrates academic focus, reflection and community service and is shown to be impactful. In Hong Kong, the first university that has made SL a graduation requirement is the Hong Kong Polytechnic University (the University). Considering this, new SL courses have proliferated over the past decade. Adopting a narrative inquiry approach, this paper examines personal narratives from a new SL subject aiming to raise awareness of refugees in Hong Kong. The data includes students’ reflective journals, co-created personal narratives and podcasts and semi-structured interviews.

Findings
This paper finds that crafting and recording narratives of shared experiences deepens cultural understanding, cultivates empathy and facilitates language learning in a genuine setting.

Social implications
Ultimately, this paper advocates a well-designed SL that combines language, content and technology as a powerful, transformational experience for both college students and service recipients.

Originality/value
This paper focuses on a brand new SL course, “Storytelling for Understanding: Refugee Children in Hong Kong,” offered in Semester 1, 2022–2023. The subject was developed by the two authors from a language division affiliated to the University. The deliverables were podcast recordings, co-authored and co-edited by the students and the children.
Untouchable them: the effect of chatbot gender on angry customers
Authors: Liang Shichang , Li Rulan , Lan Bin , Chu Yuxuan , Zhang Min , Li Li 
Published: February 2024

PurposeThis study explores how chatbot gender and symbolic service recovery may improve the satisfaction of angry customers in the context of service failures. It provides a strategy for companies to deploy chatbots effectively in customer anger.Design/methodology/approachThis research relies upon a systematic literature review to propose three hypotheses, and we recruit 826 participants to examine the effect of chatbot gender on angry customers through one lab study and one field study.FindingsThis research shows that female chatbots are more likely to increase the satisfaction of angry customers than male chatbots in service failure scenarios. In addition, symbolic recovery (apology vs. appreciation) moderates the effect of chatbot gender on angry customers. Specifically, male (vs. female) chatbots are more effective in increasing the satisfaction of angry customers when using the apology method, whereas female (vs. male) chatbots are more effective when using the appreciation method.Originality/valueThe rapid advancements in artificial intelligence technology have significantly enhanced the effectiveness of chatbots as virtual agents in the field of interactive marketing. Previous research has concluded that chatbots can reduce negative customer feedback following a service failure. However, these studies have primarily focused on the level of chatbot anthropomorphism and the design of conversational texts, rather than the gender of chatbots. Therefore, this study aims to bridge that gap by examining the effect of chatbot gender on customer feedback, specifically focusing on angry customers following service failures.