bluuebunny/crossref_metadata_2025_split huggingface dataset:
Colorimetric detection of formaldehyde using gold nanoparticles modified with 4-aminophenyl sulfone
Authors:   , Kritanusorn Arnupab 
Published: November 2023

This research aimed to develop a colorimetric method for the determination of formaldehyde on a paper-based device, using 4-aminophenyl sulfone for surface modification of gold nanoparticles (AuNPs) with an amine group that can react with formaldehyde in the presence of hydroxylamine hydrochloride. The process resulted in the aggregation of modified gold nanoparticles (modified-AuNPs) induced by formaldehyde in the presence of hydroxylamine hydrochloride. To verify this aggregation, the modified-AuNPs were characterized using ultraviolet-visible spectroscopy (UV-vis) and Transmission electron microscopy (TEM). This method offers a high sensitivity and selectivity for the determination of formaldehyde over other chemical and ions. The color change of modified-AuNPs from red to blue can be monitored by naked eyes. The relevant calibration curves was linear in the range of 40 - 110 µg mL-1 (R2 = 0.9979) and the limit of detection was found to be 35 µg mL-1 by using ImageJ software for image processing. This developed method was applied to detect formaldehyde in real samples (e.g. food and environmental), which the results were in good agreement with those obtained from the standard methods.
Development of microfluidic paper-based analytical device for determination of creatinine in urine samples
Authors:   , Sununta Suphanan 
Published: November 2023

The simple, low-cost and portable microfluidic paper-based analytical devices (µPADs) for colorimetric determination of creatinine in urine samples were developed. The methodology was based on Jaffé reaction between the creatinine and picric acid in alkaline medium generating a colorimetric creatinine-alkaline picrate complex. The product exhibits an orange color which is clearly visible on µPADs. The color intensity of the complex which is indicative of the concentration of creatinine is then quantitatively determined using ImageJ software. Various experimental parameters were optimized, i.e., concentration of picric acid, concentration of sodium hydroxide, and reaction time, and the best analytical performance was achieved with 0.04 M picric acid, 2 M sodium hydroxide, and 25 minutes of reaction time. Under the optimum conditions, a wide linear range was obtained in the range of 0.2 – 1 mM with a limit of detection (LOD) and a limit of quantitation (LOQ) of 0.08 mM and 0.26 mM, respectively. Moreover, this method provided a selective determination of creatinine and showed a good reproducibility with a precision of 2.85, 1.10 and 1.64 %RSD for the detection of creatinine at 0.2, 0.6 and 1 mM, respectively. The accuracy of the proposed method was not significantly different from the standard Jaffé method by a paired t-test at 95% confidence interval. Finally, the developed devices were successfully applied for the determination of creatinine in urine samples with %recoveries in the range of 91.06-102.86%.
Synthesis of magnetic calcium phosphate composites using citric acid as additive for removal of cadmium from aqueous solution
Authors:   , Hoang Trang 
Published: November 2023

Magnetic calcium phosphate composites were successfully synthesized in the presence of citric acid via one-pot synthesis. The phase of calcium phosphates was normally found as hydroxyapatite phase (Hap). However, the metastable phase of amorphous calcium phosphate (ACP) could occur in the presence of preformed Fe3O4 particles and citric acid. Fe3O4 might prevent the aggregation of calcium phosphate nuclei, and citric acid was found to inhibit the transformation of ACP into Hap. The inhibiting ability of citric acid depended strongly on the species of citric acid in the solution. The fully-deprotonate form promoted Hap, while the less deprotonated species yielded ACP. The materials were characterized by XRD, FTIR, elemental analysis, DSC, surface area analyzer, TEM, SEM, and VSM. Under external magnetic field, the used adsorbents were removed easily from aqueous solution showing high potential for convenient separation.

Among the prepared magnetic calcium phosphate composites, the ACP/Fe3O4/citric sample having Ca/P ratio of 1.5 shown the highest cadmium capacity. The adsorption isotherm and kinetics of this adsorbent were fitted with Langmuir model and pseudo-second-order model, respectively. The maximum adsorption capacity achieved from the Langmuir model was 270.3 mg g-1. The sorption mechanism was found to be cation exchange between Ca2+ in calcium phosphate materials and Cd2+ in the solution. Higher cadmium capacity was achieved from the materials having lower crystallinities. The cadmium adsorption capacity increased when the initial pH of the Cd2+ solution increased from 2 to 3, and remained constant between pH of 3 to 7.
In vitro biological properties of phyllanthus emblica, manilkara zapota extracts and their combinations for cosmetic applications
Authors:   , Sakdawattanakul Rapeekan 
Published: November 2023

The changes in skin conditions involve several pathways. Natural extract has been included to improve the skin condition. However, single extract with several properties is barely available. The purpose of this study is to investigate the effect of individual amla and sapota fruit extracts and their combinations at the ratio of 5:1, 1:1 and 1:5 on in vitro antioxidant, anti-inflammatory, anti-collagenase, anti-elastase and anti-tyrosinase activities. The antioxidant and anti-inflammatory activities were determined by DPPH radical scavenging assay and by nitric oxide radical scavenging assay, respectively. Anti-collagenase, anti-elastase and anti-tyrosinase were analyzed by EnzChek® gelatinase/collagenase assay kit, EnzChek® elastase assay kit and DOPA chrome method, respectively. Ethanolic amla extract exhibited the strongest antioxidant and anti-inflammatory activities with IC50 of 1.84 ± 0.1 and 17.31 ± 0.37 µg/ml, respectively, while ethanolic sapota eaxtract revealed the highest anti-collagenase and anti-elastase activities with IC50 of 65.68 ± 3.63 and 36.82 ± 0.72 µg/ml, respectively. For anti-tyrosinase activity, sapota extract showed low activity with IC50 of 5862.52 ± 169.5 µg/ml whereas it was undetectable in amla extract. Combination amla and sapota extract at the ratio of 5:1, 1:1 and 1:5 provided antioxidant activity with IC50 of 2.21 ± 0.15, 3.13 ± 0.06 and 7.3 ± 0.73 µg/ml, respectively, and showed anti-inflammatory activity with IC50 of 19.72 ± 0.35, 31.14 ± 0.49 and 68.8 ± 1.35 µg/ml, respectively. Combination containing amla as a main constituent showed the highest activities. For anti-proteinase activity, their combination at the ratio of 5:1, 1:1 and 1:5 exhibited anti-collagenase activity with IC50 of 78.9 ± 3.42, 74.48 ± 1.61 and 68.68 ± 2.69 µg/ml, respectively, and revealed anti-elastase activity with IC50 of 150.72 ± 7.9, 71.9 ± 5.06 and 36.22 ± 0.99 µg/ml, respectively. Sapota showed a predominant in both activities and the combination which contained sapota as a main constituent exhibited the highest in both activities. The combination index (CI) at median condition of most combination ratios were exhibited additive effect, except combination 1:1 in antioxidant, combination 5:1 and 1:5 in anti-inflammatory, combination 5:1​ in anti-collagenase and combination 1:5 in anti-elastase activities which showed synergistic effect. Interestingly, combination 1:5 provided potent antioxidant and good anti-inflammatory activities from the effect of amla extract, moreover, it obtained effective anti-elastase and good anti-collagenase from the effect of sapota extract. This combination, therefore, may enhance overall cosmetic properties and may be further included as an active ingredient in cosmetic product.
Effect of calcium carbonate nanoparticles on in vitro photoprotection efficiency of physical sunscreens
Authors:   , Yuensook Wachiraphon 
Published: November 2023

Physical sunscreens are safer, more photostable and broader spectral protection but exhibit lower SPF than chemical sunscreens. In this study, calcium carbonate nanoparticles were combined with both titanium dioxide and zinc oxide to improve UV protection efficiency. Speed and duration of high speed disperser were varied to investigate suitable dispersing condition in dimethicone oil. The results showed that speed of 2000 rpm and duration of 10 minutes was the optimal condition.

Concentrations of physical sunscreens and calcium carbonate were varied to study the UV improving protection using UV-Visible spectrophotometer. It was found that addition of calcium carbonate can improve UV absorption in every physical sunscreen concentrations. The enhancing results were confirmed by evaluating the sun protection factor SPF and UVA protection factor UVA-PF values using SPF analyzer. Titanium dioxide combined with calcium carbonate resulted in synergistic effect on UV protection whereas the combination of zinc oxide and calcium carbonate exhibited only additive effect. Therefore, titanium dioxide combined with calcium carbonate was chosen to be formulated in water in oil (w/o) and oil in water (o/w) emulsions to investigate for suitable emulsion type. The results showed that w/o had better SPF and UVA-PF values than o/w (p<0.05). In conclusion, calcium carbonate nanoparticles can be used as photoprotection enhancer for both physical sunscreens. The enhancement was especially high for titanium dioxide where it can reduce the level of titanium dioxide used in the formulation.
The impact of learning-centered leadership on teacher engagement in professional learning in Thailand
Authors:   , Piyaman Patnaree 
Published: November 2023

This study explores the role of learning-centered leadership in fostering teacher trust, teacher agency, and teacher engagement in professional learning in Thailand. The research employed a mixed method with sequential explanatory design. Quantitative analysis employed confirmatory factor analysis and structural equation modeling to define the measurement model. Qualitative research is conducted through an in-depth interview and focus-groups with principals, middle-level leaders, and teachers to elaborates on the quantitative findings and to gain further insight about the relationship of principal leadership and teacher learning. Research results affirmed the role of learning-centered leadership in fostering teacher engagement in professional learning and highlighted teacher trust and teacher agency as the two significant mediators. The study also suggests human resource gap between urban and rural schools. In addition, the research introduces new model of leadership for teacher learning in Thailand, which consists of eight practices: Teacher/Leader collaboration, Open/Supportive environment, Support through various sources/approaches, Internal/External learning opportunities, Coaching/Mentoring program, Teacher research program, Continuous evaluation system, and Cultural competency.
Analysis of physiological and psychological responses to odor stimulation
Authors:   , Lin Yan 
Published: November 2023

Heart rate variability (HRV) analyses as well as Stress Response Scale-18 (SRS-18) questionnaire were used to evaluate the effect of Jasmine-odor inhalation in males and females. Healthy 15 males (26.3 ± 3.7 years) and 15 females (30.1 ± 3.4 years) participated in the experiment. Standard Lead-II ECG data were collected for resting, control and odor stimulation conditions. The instantaneous heart rate variability tachogram was interpolated with the use of cubic spline interpolation, and then, FFT was performed to find the HRV power spectrum.

HRV frequency domain analysis parameters were low frequency range (LF: 0.04 - 0.15Hz), high frequency range (HF: 0.15 - 0.4 Hz) and the ratio of normalized low frequency to high frequency (nLF/nHF). HRV time domain analysis parameters in this research were heart rate (bpm), the standard deviation of all normal to normal RR intervals (SDNN) and root mean square of successive RR intervals difference (RMSSD).

Experimental results suggest that stress of both males and females decreased after exposing to odor (p<0.05). It was also found that heart rate decreasing rate of males was faster than that of females for odor sets. SDNN and RMSSD parameter were slightly increased in both odor and control experiment but not so much difference in both males and females. The nLF/nHF ratio of males are significantly higher than females in all measurement intervals (p<0.05). This might be intrinsic character of males and females. After odor exposure, the nLF/nHF ratio was slightly decreased in male subjects while it was slightly increased for all control intervals. Decreasing the nLF/nHF ratio might link to decreasing the stress. The nLF/nHF ratio in female subjects was not so much changed in both odor and control intervals with slightly increased trend. The results indicate that ECG measurement could be used to evaluate the stress state quantitatively. It will be alternative technique for stress evaluation other than using the qualitative measurement with SRS scale.
RELEVANSI INERANSI KITAB YOEL 2:28-32 TERHADAP NUBUATAN MASA KINI
Authors: Suhartono Tony , Situmorang Kasten , Sitohang Hendry 
Published: November 2023

The purpose of this study is to explain the importance of the inerrancy of the Bible from the author's point of view, prove that the fulfillment of the prophecy of Joel 2:28-32 and find out the relevance of the gift of prophecy today with qualitative descriptive research methods, where data collection techniques are by means of literature study and testimony. Writer. The findings and conclusions (1) that the inerrancy of the Bible is very important to be studied by believers, because it has implications for all areas of life. (2) The prophecy of the book of Joel 2:28-32 began to be fulfilled at Pentecost when the disciples received the outpouring of the Holy Spirit and will end until the perfect one comes the second time, namely Jesus Christ the Lord. (3) The Bible never states that prophecy ends, if the Lord Jesus has not come a second time. So the author concludes that prophecy still exists today and is very much needed in building and maturing God's congregation.
Keywords: Inerrancy, Prophecy, Present
ETIKA KRISTEN MENJADIKAN UMAT YANG BERINTEGRITAS
Authors: Suriyanti Intan , Steven  
Published: November 2023

Ethics and integrity are two things that cannot be separated. People who are know work ethics and do it with all their heart, can be ascertained as a person of integrity. As Christians, the Bible as the word of God and Jesus Christ as an example are the foundation of Christian ethics. In this case, ethics is considered as a means of orientation for human efforts to live and act. For Christians living in Indonesia with its various diversity is challenging, living as the light and the salt is a must to be able to witnessing the truth of God's Word. So that all those who do not know and not interested in knowing Him can be changed into His followers. The truth of God's word can be seen clearly, if someone has and know Christian ethics and live with it. This ethic which refers to the lifestyle of followers of Christ and His Word is the standard for Christian morals and lifestyle. And when they do it honestly and righteously they can be called Christians with Integrity. At the end of 2020, the GBI church under the guidance of Pastor Niko Njotoraharjo declared 2021 as the "year of Integrity". Of course, The Hope is that every congregations, workers, especially pastors can live with integrity,and following the life of Jesus Christ while living in the world.
Place et enjeux de la sémiologie dans la formation et l’enseignement
Authors: Lebreton Reinhard Maud , Kohler Alaric 
Published: November 2023

À l’aune d’une accessibilité accrue du savoir, la recherche en éducation invite à replacer la réflexivité des apprenant·e·s au centre des objets d’apprentissage, notamment grâce à l’approche par compétences, forçant ainsi la prise en compte de la construction individuelle de sens.
Cette contribution s’appuie sur deux pratiques de formation d’enseignant·e·s fondées sur la sémiologie et menées au sein de la même institution de formation en Suisse. En problématisant les enjeux d’une formation à la sémiologie, les auteur·e·s présentent les traces des activités menées avec les futur·e·s enseignant·e·s avant de proposer des pistes communes sur les formes et les finalités que peut prendre une sémiologie orientée vers la formation et l’éducation.
S’appuyant sur deux interventions différentes, en didactique du français et en psychologie de l’apprentissage, cette contribution montre que la perspective sémiotique1 constitue un outil au service de l’analyse de la pratique enseignante, du choix de la posture professionnelle, de la gestion des supports multimodaux, du développement de l’interdisciplinarité et de la pensée critique.
Dépassant le constat que l’enseignement-apprentissage dépend de la (co-)construction de sens, les auteur·e·s, sur la base de leurs expériences respectives, amorcent la réflexion d’une compétence sémiotique à construire chez les enseignant·e·s, grâce au développement d’une sémiologie à la fois issue de et orientée vers la pratique.
De la lecture à l’appropriation d’un livre enrichi : enquête auprès d’un public d’étudiants
Authors: Daniel Catherine , Camussi Marie-Armelle 
Published: November 2023

Cet article rend compte des résultats d’une enquête de réception de l’édition numérique enrichie de La Croisade des Enfants de Marcel Schwob. S’appuyant sur l’expérience de conception de l’ouvrage, menée de façon collaborative par l’équipe « Lectures et médiations numériques » (CELLAM-Rennes 2) et en collaboration avec l’équipe du Loustic (Rennes, le groupe a mené une enquête croisant des données quantitatives et qualitatives auprès de 44 étudiants visant d’une part, à évaluer la motivation suscitée par l’édition enrichie d’un livre numérique en comparaison à sa version non enrichie, d’autre part à analyser la façon dont les lecteurs se sont emparés de la version enrichie et les postures de lecteur qu’elle a inspirées. L’article se focalise sur l’appropriation du livre enrichi pour évaluer le degré et le type d’appropriation effectuée par les enquêtés. Les résultats sont assez éloignés des attentes des concepteurs, les postures des lecteurs s’étant surtout concentrées autour d’une lecture contextualisante. Au total, les résultats de l’enquête permettent de s’interroger sur les critères favorisant l’appropriation d’un livre enrichi, parmi lesquels le poids des habitudes de lecture et du discours sur la légitimité culturelle du livre numérique enrichi semblent prédominants. Ces résultats conduisent à préconiser différents types de médiation qui permettraient au lecteur d’exploiter toutes les potentialités de cet objet éditorial.
Oncogenic KRAS, Mucin 4, and Activin A‐Mediated Fibroblast Activation Cooperate for PanIN Initiation
Authors: Hu Chun‐Mei , Huang Chien‐Chang , Hsu Min‐Fen , Chien Hung‐Jen , Wu Pei‐Jung , Chen Yi‐Ing , Jeng Yung‐Ming , Tang Shiue‐Cheng , Chung Mei‐Hsin , Shen Chia‐Ning , Chang Ming‐Chu , Chang Yu‐Ting , Tien Yu‐Wen , Lee Wen‐Hwa 
Published: November 2023

AbstractOver 90% of patients with pancreatic ductal adenocarcinoma (PDAC) have oncogenic KRAS mutations. Nevertheless, mutated KRAS alone is insufficient to initiate pancreatic intraepithelial neoplasia (PanIN), the precursor of PDAC. The identities of the other factors/events required to drive PanIN formation remain elusive. Here, optic‐clear 3D histology is used to analyze entire pancreases of 2‐week‐old Pdx1‐Cre; LSL‐KrasG12D/+ (KC) mice to detect the earliest emergence of PanIN and observed that the occurrence is independent of physical location. Instead, it is found that the earliest PanINs overexpress Muc4 and associate with αSMA+ fibroblasts in both transgenic mice and human specimens. Mechanistically, KrasG12D/+ pancreatic cells upregulate Muc4 through genetic alterations to increase proliferation and fibroblast recruitments via Activin A secretion and consequently enhance cell transformation for PanIN formation. Inhibition of Activin A signaling using Follistatin (FST) diminishes early PanIN‐associated fibroblast recruitment, effectively curtailing PanIN initiation and growth in KC mice. These findings emphasize the vital role of interactions between oncogenic KrasG12D/+‐driven genetic alterations and induced microenvironmental changes in PanIN initiation, suggesting potential avenues for early PDAC diagnostic and management approaches.
High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice Model
Authors: Huang Hsin , Hsu Pei‐Ling , Tsai Sheng‐Feng , Chuang Yi‐Hsiang , Chen De‐Quan , Xu Guo‐Xuan , Chen Chien , Kuo Yu‐Min , Huang Chih‐Chung 
Published: November 2023

AbstractAlthough the relationships of cerebrovascular hemodynamic dysfunction with neurodegenerative diseases remain unclear, many studies have indicated that poor cerebral perfusion accelerates the progression of neurodegenerative diseases, such as Alzheimer's disease (AD). Small animal models are widely used in AD research. However, providing an imaging modality with a high spatiotemporal resolution and sufficiently large field of view to assess cerebrovascular hemodynamics in vivo remains a challenge. The present study proposes a novel technique for high‐spatiotemporal‐resolution vector micro‐Doppler imaging (HVμDI) based on contrast‐free ultrafast high frequency ultrasound imaging to visualize the cerebrovascular hemodynamics of the mouse, with a data acquisition time of 0.4 s, a minimal detectable vessel size of 38 µm, and a temporal resolution of 500 Hz. In vivo experiments are conducted on wild‐type and AD mice. Cerebrovascular hemodynamics are quantified using the cerebral vascular density, diameter, velocity, tortuosity, cortical flow pulsatility, and instant flow direction variations. Results reveal that AD significantly change the cerebrovascular hemodynamics. HVμDI offers new opportunities for in vivo analysis of cerebrovascular hemodynamics in neurodegenerative pathologies in preclinical animal research.
Deep Learning Enables Rapid Identification of a New Quasicrystal from Multiphase Powder Diffraction Patterns
Authors: Uryu Hirotaka , Yamada Tsunetomo , Kitahara Koichi , Singh Alok , Iwasaki Yutaka , Kimura Kaoru , Hiroki Kanta , Miyao Naoya , Ishikawa Asuka , Tamura Ryuji , Ohhashi Satoshi , Liu Chang , Yoshida Ryo 
Published: November 2023

AbstractSince the discovery of the quasicrystal, approximately 100 stable quasicrystals are identified. To date, the existence of quasicrystals is verified using transmission electron microscopy; however, this technique requires significantly more elaboration than rapid and automatic powder X‐ray diffraction. Therefore, to facilitate the search for novel quasicrystals, developing a rapid technique for phase‐identification from powder diffraction patterns is desirable. This paper reports the identification of a new Al–Si–Ru quasicrystal using deep learning technologies from multiphase powder patterns, from which it is difficult to discriminate the presence of quasicrystalline phases even for well‐trained human experts. Deep neural networks trained with artificially generated multiphase powder patterns determine the presence of quasicrystals with an accuracy >92% from actual powder patterns. Specifically, 440 powder patterns are screened using the trained classifier, from which the Al–Si–Ru quasicrystal is identified. This study demonstrates an excellent potential of deep learning to identify an unknown phase of a targeted structure from powder patterns even when existing in a multiphase sample.