bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single cell analysis reveals an antiviral network that controls Zika virus infection in human dendritic cells
Authors: Moore Kathryn M. , Pelletier Adam-Nicolas , Lapp Stacey , Metz Amanda , Tharp Gregory K. , Lee Michelle , Bhasin Swati Sharma , Bhasin Manoj , Sékaly Rafick-Pierre , Bosinger Steven E. , Suthar Mehul S. 
Published: January 2024

SummaryZika virus (ZIKV) is a mosquito-borne flavivirus that caused an epidemic in the Americas in 2016 and is linked to severe neonatal birth defects, including microcephaly and spontaneous abortion. To better understand the host response to ZIKV infection, we adapted the 10x Genomics Chromium single cell RNA sequencing (scRNA-seq) assay to simultaneously capture viral RNA and host mRNA. Using this assay, we profiled the antiviral landscape in a population of human moDCs infected with ZIKV at the single cell level. The bystander cells, which lacked detectable viral RNA, expressed an antiviral state that was enriched for genes coinciding predominantly with a type I interferon (IFN) response. Within the infected cells, viral RNA negatively correlated with type I IFN dependent and independent genes (antiviral module). We modeled the ZIKV specific antiviral state at the protein level leveraging experimentally derived protein-interaction data. We identified a highly interconnected network between the antiviral module and other host proteins. In this work, we propose a new paradigm for evaluating the antiviral response to a specific virus, combining an unbiased list of genes that highly correlate with viral RNA on a per cell basis with experimental protein interaction data. Our ZIKV-inclusive scRNA-seq assay will serve as a useful tool to gaining greater insight into the host response to ZIKV and can be applied more broadly to the flavivirus field.
Healthy Japanese Dietary Pattern Is Associated with Slower Biological Aging in Older Men: WASEDA’S Health Study
Authors: Kawamura Takuji , Higuchi Mitsuru , Ito Tomoko , Kawakami Ryoko , Usui Chiyoko , McGreevy Kristen M. , Horvath Steve , Zsolt Radak , Torii Suguru , Suzuki Katsuhiko , Ishii Kaori , Sakamoto Shizuo , Oka Koichiro , Muraoka Isao , Tanisawa Kumpei 
Published: January 2024

AbstractAging is the greatest risk factor for numerous diseases and mortality, and establishing geroprotective interventions targeting aging is required. Previous studies have suggested that healthy dietary patterns, such as the Mediterranean diet, are associated with delayed biological aging; however, these associations depend on nationality and sex. Therefore, this study aimed to investigate the relationship between dietary patterns identified through principal component analysis and biological aging in older men of Japan, one of the countries with the longest life expectancies. Principal component analysis identified two dietary patterns: a healthy Japanese dietary pattern and a Western-style dietary pattern. Eight epigenetic clocks, some of the most accurate aging biomarkers, were identified using DNA methylation data from whole-blood samples. Correlation analyses revealed that healthy Japanese dietary patterns were significantly negatively or positively correlated with multiple epigenetic age accelerations (AgeAccel), including AgeAccelGrim, FitAgeAccel, and age-adjusted DNAm-based telomere length (DNAmTLAdjAge). Conversely, the Western-style dietary pattern showed no significant correlation with any of the examined epigenetic AgeAccels or age-adjusted values. After adjusting for confounders, the healthy Japanese dietary pattern remained significantly negatively correlated with AgeAccelPheno and AgeAccelGrim and positively correlated with DNAmTLAdjAge. These findings suggest that a Western-style dietary pattern is not associated with biological aging, whereas a healthy Japanese dietary pattern is associated with delayed biological aging in older Japanese men. Our findings provide evidence that healthy dietary patterns may have beneficial effects on delayed biological aging in older Japanese men.
Microplastics from disposable paper cups are enriched in the placenta and fetus, leading to metabolic and reproductive toxicity during pregnancy
Authors: Chen Qiong , Peng Chen , Xu Haoteng , Su Zhuojie , Yilihan Gulimire , Wei Xin , Shen Yueran , Jiang Chao 
Published: January 2024

AbstractThe health implications of microplastics (MPs), especially those originating from hot drinks in disposable paper cups (DPCs), are increasingly alarming. We investigated the accumulation and metabolic and reproductive toxicological effects of MPs from DPCs filled with hot water in various tissues in a pregnant mouse model. Simulating human intake of 0.3, 3.3, and 33.3 cups daily, we found MPs exposure-induced dose-responsive harmful effects on murine fetal development and maternal physiology. MPs were detected in all 13 examined tissues, with the highest accumulation in the cecal contents, followed by significant depositions in the fetus, placenta, kidney, spleen, lung, and heart. A higher proportion of smaller MPs (90.35% < 10 μm) was identified in brain tissues. Dose-responsive changes in functional microbiome and gene pathways were observed. Moderate MPs intake of 3.3 cups daily significantly altered cecal microbiome composition and metabolic functions. The transcriptomic functional variations in maternal blood, placenta, and mammary gland underscore the significant impacts of realistic MPs exposure on metabolic and immune health and posing neurodegenerative and miscarriage risks. The benchmark dose framework analysis using tissue-specific gene biomarkers revealed safe exposure limits at 2 to 4 cups/day during pregnancy. Our results indicate selective tissue accumulation and potential metabolic and reproductive toxicities of MPs at exposure levels presumed non-hazardous. Such risks remain unaddressed within current food safety regulations, impacting vulnerable groups such as pregnant women and fetuses.Research HighlightsMicroplastics released from disposable paper cups filled with hot water showed preferential accumulations in the murine fetus, placenta, kidney, spleen, lung, and heart, with significant adverse impacts on fetal development.Microplastic exposure led to dose-responsive maternal microbiome changes associated with increased fatty acid biosynthesis and elevated expressions of genes related to viral infections, neurodegenerative diseases, oxidative stress, and miscarriage risk.A consumption level of 3.3 cups/day was sufficient to elicit systemic metabolic and reproductive toxicity, with a predicted safe exposure limit of 2 to 4 cups/day during pregnancy by benchmark dose framework analysis with molecular biomarkers.
Microbial Community Structure of an Anaerobic Side-stream Coupled Anoxic-aerobic Membrane Bioreactor (AOMBR-ASSR) for an In-situ Sludge Reduction Process
Authors: Ning Xinqiang , Hu Jialun , Yue Jiao , Tang Tang , Zhang Bin 
Published: January 2024

AbstractThe in-situ sludge reduction process via the insertion of an anaerobic side-stream reactor into the sludge return circuit is an efficient approach to reduce the sludge yield in the activated sludge process. In this study, with the anoxic-aerobic membrane bioreactor (AO-MBR,CP) as a reference, high-throughput sequencing technology was used to reveal the characteristics of the microbial community structure in the anaerobic side-stream anoxic-aerobic membrane bioreactor sludge reduction process (AOMBR-ASSR,SRP). After the stable operation of two processes for 120 days, the average removal efficiencies of TN and TP in the effluent of SRP were increased by 5.63% and 29.85%, respectively. However, there was no significant difference between the two processes in the removal effect of COD and ammonia nitrogen. It is worth noting that the observed sludge yields (Yobs) of the two processes were 0.14 and 0.17 gMLSS/(gCOD), respectively, and the sludge reduction rate of the AOMBR-ASSR reduction process was 19.5%. Compared to the CP, the microbial richness and diversity index of SRP increased significantly. Among 14 major phyla,ProteobacteriaandBacteroideteswere the dominant microorganisms.Chloroflexi, which is responsible for the degradation of organic substances under an anaerobic condition, seemed to be reduced in the SRP. Meanwhile, other phyla that involved in the nitrogen cycle, such asNitrospiraeandPlanctomycetes, were found to be more abundant in the SRP than in the CP. A total of 21 identified classes were observed, and primarily hydrolyzed fermented bacteria (Sphingobacteriia, Betaproteobacteria, Actinobacteria, andDeltaproteobacteria) and slow-growing microorganisms (Bacilli) were accumulated in the SRP. At the genus level, the inserted anaerobic side-stream reactor favored the hydrolyzed bacteria (Saprospiraceae, RhodobacterandCandidatus_Competibacter), fermented bacteria (LactococcusandTrichococcus), and slow-growing microorganisms (DechloromonasandHaliangium), which play a crucial role in the sludge reduction. Furthermore, the enrichment of bacterial species related to nitrogen (NitrospirandAzospira) provided the potential for nitrogen removal, while the anaerobic environment of the side-stream reactor promoted the enrichment of phosphorus-accumulating organisms.
Measuring quality-adjusted price changes on the mobile market: a case study from Hungary
Authors: Pápai Zoltán , Nagy Péter , McLean Aliz 
Published: January 2024

Purpose
This study aims to estimate the quality-adjusted changes in residential mobile consumer prices by controlling for the changes in the relevant service characteristics and quality, in a case study on Hungary between 2015 and 2021; compare the results with changes measured by the traditionally calculated official telecommunications price index of the Statistical Office; and discuss separating the hedonic price changes from the effect of a specific government intervention that occurred in Hungary, namely, the significant reduction in the value added tax rate (VAT) levied on internet services.

Design/methodology/approach
Since the price of commercial mobile offers does not directly reflect the continuous improvements in service characteristics and functionalities over time, the price changes need to be adjusted for changes in quality. The authors use hedonic regression analysis to address this issue.

Findings
The results show significant hedonic price changes over the observed seven-year period of over 30%, which turns out to be primarily driven by the significant developments in the comprising service characteristics and not the VAT policy change.

Originality/value
This paper contributes to the literature on hedonic price analyses on complex telecommunications service plans and enhances this methodology by using weights and analysing the content-related features of the mobile packages.
Role of brand attachment and satisfaction in driving customer behaviors for durables: a longitudinal study
Authors: Guru Ramesh Roshan Das , Paulssen Marcel , Japutra Arnold 
Published: January 2024

Purpose
This study aims to extend research in marketing on two important relational constructs, customer satisfaction and brand attachment, by comparing their long-term effects on customer behaviors with different levels of performance difficulty in a relatively understudied domain of durable products.

Design/methodology/approach
Using a two-stage quantitative study with US customers from five durable product categories, the authors first explored the hierarchy of customers’ loyalty behaviors based on increasing effort in a pretest study (N = 675). Then, the authors tested the effectiveness of satisfaction and brand attachment for customers’ loyalty behaviors over a nine-month period in a longitudinal study (N = 2,284) with customers from the same product categories.

Findings
Compared to satisfaction, brand attachment emerges as a stronger long-term predictor of customer behaviors. The performance difficulty of customer behaviors positively moderates the impact of brand attachment and negatively moderates the impact of customer satisfaction. Brand attachment is particularly effective in predicting difficult-to-perform customer behaviors, which require customers to expend resources such as time and money. Customer satisfaction is mainly effective for predicting easy-to-perform behaviors, but its long-term impact is significantly lower for easy-to-perform behaviors than brand attachment.

Research limitations/implications
The use of consumer durables in the study and samples from only one country restricts the generalizability of the findings.

Practical implications
The complementary roles of customer satisfaction and brand attachment are highlighted. Only satisfying customers is not enough to engage customers in behaviors that require resources such as money, time and energy for the brand.

Originality/value
A comparative study on the long-term effectiveness of two established relational metrics in explaining different customer behaviors varying in their performance difficulty in an understudied domain of durable products.
Convexity of the triple helix of innovation game
Authors: Mêgnigbêto Eustache 
Published: January 2024

Purpose
This paper aims to determine the conditions for the core of the Triple Helix game to exist. The Triple Helix of university-industry-government relationships is a three-person cooperative game with transferable utility. Then, the core, the Shapley value and the nucleolus were used as indicators of the synergy within an innovation system. Whereas the Shapley value and the nucleolus always exist, the core may not.

Design/methodology/approach
The core of a three-person cooperative game with transferable utility exists only if and only if the game is convex. The paper applies the convexity condition to the Triple Helix game.

Findings
The Triple Helix game is convex if and only if there is output within the system; it is strictly convex if and only if all the three bilateral and the trilateral relationships have an output.

Practical implications
Convex games are competitive situations in which there are strong incentives towards the formation of large coalitions; therefore, innovation actors must cooperate to maximise their interests. Furthermore, a Triple Helix game may be split into subgames for comprehensive analyses and several Triple Helix games may be combined for a global study.

Originality/value
This paper extends the meaning of the Shapley value and the nucleolus for Triple Helix innovation actors: the Shapley value indicates the quantity a player wins because of the coalitions he involves in and the nucleolus the return for solidarity of an innovation actor.
Examining the advantages and disadvantages of international franchising and licensing from both legal and business perspectives within GCC region
Authors: Al Bakri Anas A. , Kisswani Nazzal M. 
Published: January 2024

Purpose
This study aims to provides the insights on the advantages and disadvantages of international franchising and licensing from the perspectives of legal and business considerations in the Gulf Cooperation Council (GCC).

Design/methodology/approach
Using a quantitative research approach, the authors conducted a survey with 150 business owners and franchisees in the GCC and analyzed the data using descriptive statistics, structural equation modeling and frequency analysis.

Findings
The findings reveal that while international franchising and licensing offer significant benefits for business expansion and revenue growth, they also pose risks related to legal compliance, cultural differences and intellectual property protection. Indeed, the results of this study provide valuable insights into the advantages and disadvantages of international franchising and licensing in the GCC from both legal and business perspectives.

Originality/value
There is limited research on the legal and business perspectives of international franchising and licensing in the GCC. This study contributes to the literature by providing a comprehensive analysis of the legal and business perspectives of international franchising and licensing in the GCC.
Examining body mass index and health-related fitness marker progression of incarcerated minority youth engaged in a sport-leadership program
Authors: Wahl-Alexander Zachary , Jacobs Jennifer , Hill Christopher M. , Bennett Gabrielle 
Published: January 2024

Purpose
The purpose of this study was to examine the impact of a sport-leadership program on minority incarcerated young adults’ health-related fitness markers.

Design/methodology/approach
This study occurred at an all-male juvenile detention center. A total of 41 participants in this study were obtained from a sample of 103 incarcerated young adults. Data collection entailed body mass index (BMI) evaluation, cardiovascular endurance tests and 1-min pushups and situps at two different time periods (before and after three months). A 2 × 2 mixed factorial analysis of variances was used to test for differences among the within subjects’ factors (time [pre × post]) and between subjects’ factors (groups [flex × control]) for the above-mentioned dependent variables.

Findings
Over the course of three consecutive months of engagement, preliminary indications demonstrated participants had a slight reduction in BMI and significant increases in cardiovascular endurance and muscle strength. Contrarily, during this same time period, non-participating young adults exhibited significant increases in BMI and decreases in cardiovascular endurance and muscle strength.

Originality/value
Integration of sport-leadership programs is generally not free but can be a low-cost alternative for combatting many issues surrounding physical activity, weight gain and recreational time for those incarcerated.
When leadership goes awry: the nexus between tyrannical leadership and knowledge hiding
Authors: Guo Maohong , Khassawneh Osama , Mohammad Tamara , Pei Xintian 
Published: January 2024

Purpose
Grounded on the conservation of resources (COR) theory, this study examines the relationship between tyrannical leadership and knowledge hiding. Additionally, this study aims to investigate the mediating role of psychological distress and the moderating role of psychological safety.

Design/methodology/approach
Data was gathered from 435 employees in the corporate sector in China. The study used the partial least squares structural equation modelling approach to assess the proposed connections and analysed the data collected with the help of SmartPLS 4 software.

Findings
In the study, it was found that there is a positive relationship between tyrannical leadership and knowledge hiding, and this association is mediated by psychological distress. Additionally, the results asserted that the positive effect of tyrannical leadership on knowledge hiding through psychological distress is less pronounced when there is a greater degree of psychological safety.

Practical implications
Leaders should avoid being tyrannical and adopt a supportive leadership style. They should be aware of the effects of their behaviour on employee well-being, provide resources to help employees cope with distress and foster a culture of psychological safety. This approach promotes knowledge sharing, innovation and employee well-being within the organisation.

Originality/value
This study contributes to the existing literature by investigating a new factor that influences knowledge hiding: tyrannical leadership. Furthermore, it explains that employees who experience tyrannical leadership are more prone to psychological distress, such as anxiety and fear, and are likelier to engage in knowledge-hiding behaviours. Finally, the study identifies psychological safety as a factor that can mitigate the negative effects of tyrannical leadership on knowledge hiding.
Resilience dynamics and their determinants: the transition to coronavirus pandemic recovery
Authors: Duro Juan Antonio , Fernández-Fernández Melchor , Perez-Laborda Alejandro , Rosselló Jaume 
Published: January 2024

Purpose
This study aims to introduce a dynamic perspective of tourism resilience by analyzing tourism demand in Spain during the 2020 and 2021 summers in the context of the COVID-19 pandemic.

Design/methodology/approach
This study uses regression and Lasso-type methods to demonstrate a great explanatory capacity of past determinants to explain the tourism demand of the Spanish provinces.

Findings
Results show how the previous specialization of the domestic market, the density and the geographic location related to the type of product are behind the bulk of the territorial differences in demand resilience, although in 2021 there has been a process of adaptation to the new context.

Research limitations/implications
This study contributes to the theoretical understanding of tourist behavior and tourism destination management by introducing the concept of resilience dynamics of destinations.

Practical implications
The results are useful, on the one hand, for tourist destinations to understand the different stages of recovery from a shock, and on the other hand, to go in deep in consumer behavior after a shock.

Originality/value
These findings represent a paradigm shift in the static conception of resilience in tourism.
Impact of forest fragmentation and associated edge effects on the population density of four nocturnal lemur species in North West Madagascar
Authors: Hending D. , Randrianarison H. , Andriamavosoloarisoa N. N. M. , Ranohatra‐Hending C. , McCabe G. , Cotton S. , Holderied M. 
Published: January 2024

AbstractThe clearing and fragmentation of tropical forests is the single biggest threat to primate populations who depend on this habitat for survival. In contrast to primates that live in continuous, undisturbed forests, primate communities of fragmented forests need to adapt to decreased food availability and increased inter‐ and intraspecific competition typical of these degraded and anthropogenically disturbed habitats. Some primate species are highly sensitive to habitat fragmentation, whilst other species can adapt and even thrive in fragmented and degraded forests. Here, we assessed how forest fragmentation and associated edge effects impact the population density of four species of nocturnal lemur in the Sahamalaza‐Iles Radama National Park, North West Madagascar. We conducted 118 transect walks over a 3‐year period covering a total distance of 107 km to collect encounter rate (N/km) and population density (N/Ha) data for each species, which we then compared between the edge and core areas of a continuous forest and a fragmented forest. Our results were highly species‐specific, with the population densities of two species (Lepilemur sahamalaza and Microcebus sambiranensis) increasing in edge and fragmented habitat, whilst we observed the opposite for Cheirogaleus medius. Mirza zaza density appeared consistent between the continuous and fragmented forest and in both edge and core areas. We also found evidence of species‐specific population density relationships with fragment size, core area and fragment shape; however, further work is needed to support these findings. This study demonstrates that some nocturnal lemurs can adapt to degraded habitats and thrive within fragmented forests, whilst other species are less capable of doing so.