bluuebunny/crossref_metadata_2025_split huggingface dataset:
The elective use of the seven‐cell NIR stent in coronary arteries < 2.75 mm in diameter: Clinical and angiographic results at 6‐month follow‐up
Authors: Eccleshall Simon C. , Jones Heather , Lichfield Maureen , Jordan Paul J. , Buller Nigel P. , Townend Jonathan N. 
Published: August 2002

AbstractThe outcome of interventional procedures has been improved by the use of coronary stents in vessels > 3.0 mm in diameter. We report a single‐center prospective study of elective coronary stenting in 39 consecutive patients with de novo lesions and reference diameters < 2.75 mm. Seven‐cell NIR stents were deployed with standard antiplatelet therapy, but without routine use of IIb/IIIa inhibitors or intravascular ultrasound. There was one case of acute stent thrombosis, but no cases of subacute thrombosis. The binary restenosis rate was 27.8% (10/36 lesions) and the target lesion revascularization rate was 19.4%. Symptoms improved markedly as judged by Canadian Cardiovascular Society and questionnaire scores and the use of antianginal therapy. This pilot study suggests that the elective use of coronary stents in vessels < 2.75 mm in diameter is safe with acceptable long‐term results. A randomized trial comparing outcomes after stents and balloon angioplasty in small vessels is required. Cathet Cardiovasc Intervent 2001;54:174–179. © 2001 Wiley‐Liss, Inc.
Transcatheter treatment of coronary artery disease and atrial septal defect with sequential implantation of coronary stent and Amplatzer septal occluder: Preliminary results
Authors: Onorato Eustaquio , Pera Isidoro , Lanzone Alberto , Ambrosini Vittorio , Rubino Paolo , Trabattoni Daniela , Pepi Mauro , Bartorelli Antonio L. 
Published: August 2002

AbstractCoronary stent implantation had been established as a highly effective revascularization technique in patients with occlusive coronary artery disease. Transcatheter closure of atrial septal defects is becoming a definite alternative to surgery in properly selected patients. During a 19‐month period, 6 patients (50% women; mean age, 58 ± 17 years; range, 32–73 years) of a consecutive series of 176 prospective multicenter registry patients undergoing transcatheter atrial septal defect closure were treated with sequential percutaneous coronary revascularization and Amplatzer septal occluder implantation. Indication for revascularization was stable angina in four patients and unstable angina in two. Indication for defect closure was significant left‐to‐right shunt with right ventricular enlargement. Defect diameter ranged from 13 to 20 mm by transesophageal echocardiography, and the stretched diameter measured 13 to 25 mm. Procedural success of both interventions was achieved in all cases without in‐hospital complications. A total of seven stents were successfully implanted in five coronary vessels. No stent was used in one patient after successful PTCA. Immediate total closure of the defect was obtained in five patients. Trivial residual shunting, observed in one patient, disappeared at 24 hr. No adverse cardiac events, recurrence of anginal symptoms, or evidence of residual shunt were observed at clinical and echocardiographic follow‐up, which ranged from 60 to 390 days (mean, 258 ± 150 days). These results suggest that sequential transcatheter therapy of coronary artery disease and atrial septal defect is safe and efficacious in selected patients. Cathet Cardiovasc Intervent 2001;54:454–458. © 2001 Wiley‐Liss, Inc.
Treatment of coronary artery disease in hemodialysis patients: PTCA vs. stent
Authors: Malanuk Robert M. , Nielsen Christopher D. , Theis Paul , Assey Michael E. , Usher Bruce W. , Leman Robert B. 
Published: August 2002

AbstractThis retrospective study examined the utilization of coronary stent placement versus PTCA in hemodialysis patients with obstructive coronary disease. Prior studies have demonstrated suboptimal results with PTCA owing primarily to high rates of restenosis. We identified 19 hemodialysis patients who received a percutaneous coronary intervention who were matched with individuals without renal failure undergoing same vessel revascularization. Our study found that 71% of hemodialysis patients receiving PTCA had a recurrent cardiac event defined by subsequent angina, myocardial infarction, or cardiac death. At follow‐up, 30% of patients who received stent placement had recurrent cardiac events. Intracoronary stent placement is both safe and feasible and produces more favorable clinical outcomes in the management of coronary disease in hemodialysis patients. Cathet Cardiovasc Intervent 2001;54:459–463. © 2001 Wiley‐Liss, Inc.
Could plasmid‐mediated gene transfer into the myocardium be augmented by left ventricular guided laser myocardial injury?
Authors: Fuchs Shmuel , Baffour Richard , Shou Matie , Stabile Eugenio , Singh Sandeep , Schwartz Bertrand , Branellec Didier , Epstein Stephen E. , Kornowski Ran 
Published: August 2002

AbstractEarly studies have indicated no correlation between the amount of mechanical injury and the level of myocardial gene expression following direct plasmid vector injection. Recently, however, evidence suggests that combined laser myocardial injury and plasmid‐based gene delivery exert synergistic effects on gene expression and activity. The purpose of the study was to determine whether laser‐induced myocardial injury followed by transendocardial gene transfer increases gene expression compared to gene transfer alone. We assessed the ability of a plasmid vector to express its transgene after injection into porcine ischemic myocardium with and without preceding laser myocardial injury. Thirteen animals had transendocardial injections of the luciferase reporter gene in a plamid vector using a catheter‐based injection system. Injections (0.5 mg per animal, 50 μg per injection site) were divided into 10 sites in the ischemic territory. Eight animals underwent transendocardial laser injury of the ischemic region (2 Joule per pulse × 10 sites) prior to gene delivery. In five animals, gene injection sites were dispersed between laser channels, and in three animals laser and gene delivery were applied in close proximity (< 5 mm) or at the same location. Luciferase activity was measured at 3 and 7 days. Luciferase expression in ischemic zones was markedly elevated at day 3 and 7, and similar whether animals were pretreated using laser injury followed by gene transfer compared to gene transfer alone. Neither same‐spot injection nor dispersed gene delivery were associated with augmented gene expression compared to gene transfer alone. Using the above‐described catheter‐based approach to combine localized laser injury and injection of naked DNA into ischemic myocardium, laser injury did not augment gene expression above levels present with gene transfer alone. Cathet Cardiovasc Intervent 2001;54:533–538. © 2001 Wiley‐Liss, Inc.
A systematic evaluation of the benefits and hazards of variable selection in latent variable regression. Part I. Search algorithm, theory and simulations
Authors: Baumann K. , Albert H. , von Korff M. 
Published: August 2002

AbstractVariable selection is an extensively studied problem in chemometrics and in the area of quantitative structure–activity relationships (QSARs). Many search algorithms have been compared so far. Less well studied is the influence of different objective functions on the prediction quality of the selected models. This paper investigates the performance of different cross‐validation techniques as objective function for variable selection in latent variable regression. The results are compared in terms of predictive ability, model size (number of variables) and model complexity (number of latent variables). It will be shown that leave‐multiple‐out cross‐validation with a large percentage of data left out performs best. Since leave‐multiple‐out cross‐validation is computationally expensive, a very efficient tabu search algorithm is introduced to lower the computational burden. The tabu search algorithm needs no user‐defined operational parameters and optimizes the variable subset and the number of latent variables simultaneously. Copyright © 2002 John Wiley & Sons, Ltd.
Molecular chirality and the fundamental symmetries of physics: Influence of parity violation on rovibrational frequencies and thermodynamic properties
Authors: Quack Martin , Stohner Jürgen 
Published: August 2002

AbstractWe introduce the topic of fundamental symmetries of physics in relation to molecular chirality by a brief review of the development and current status of the theory of parity violation in chiral molecules. We then discuss in some detail CHBrClF (bromochlorofluoromethane) as a test case, to which the work of André Collet has contributed importantly. For this molecule and its isotopomers, we report here the first detailed theoretical calculations of the influence of parity violation on statistical thermodynamic properties. High‐quality ab initio calculations (RPA, random phase approximation, and CASSCF, complete‐active‐space self‐consistent‐field) were performed to determine the small energy difference between R‐ and S‐enantiomers of H and D isotopomers of bromochlorofluoromethane (CHBrClF, CDBrClF), and fluorooxirane (1H3C2OF) introduced by the parity‐violating weak interaction. Together with vibrational and rotational frequency shifts caused by parity violation, these were used to determine the statistical thermodynamic quantities from the corresponding partition functions within the separable harmonic and in part also anharmonic adiabatic approximation. Temperature‐dependent equilibrium constants for the stereomutation were calculated and are discussed in relation to biochemical homochirality. Chirality 13:745–753, 2001. © 2001 Wiley‐Liss, Inc.
First direct discrimination of chiral phosphine selenide (P=Se) derivatives by multinuclear magnetic resonance spectroscopy in the presence of a chiral dirhodium complex
Authors: Malik Shahid , Duddeck Helmut , Omelanczuk Jan , Choudhary Muhammad Iqbal 
Published: August 2002

AbstractEnantiomeric ratios of compounds with P=Se functionalities (phosphine selenides) can easily be determined by 1H, 13C, 31P, and 77Se NMR spectroscopic inspection of their diastereomeric complexes with (R)‐Rh2(MTPA)4 (MTPA‐H ≡ methoxytrifluoromethylphenylacetic acid; Mosher's acid). This is the first facile and rapid spectroscopic method for chiral recognition in this class of compounds. Whereas most complexation shifts Δδ are moderate or even negligible, significant signal dispersions Δν can be observed. Some rationalization for the adduct formation mode is presented. NMR spectral characteristics of the free P=Se compounds 1–5 are described. Chirality 14:407–411, 2002. © 2002 Wiley‐Liss, Inc.
Separating centrosomes interact in the absence of associated chromosomes during mitosis in cultured vertebrate cells
Authors: Faruki Shamsa , Cole Richard W. , Rieder Conly L. 
Published: August 2002

AbstractWe detail here how “free” centrosomes, lacking associated chromosomes, behave during mitosis in PtK2 homokaryons stably expressing GFP‐α‐tubulin. As free centrosomes separate during prometaphase, their associated astral microtubules (Mts) interact to form a spindle‐shaped array that is enriched for cytoplasmic dynein and Eg5. Over the next 30 min, these arrays become progressively depleted of Mts until the two centrosomes are linked by a single bundle, containing 10–20 Mts, that persists for > 60 min. The overlapping astral Mts within this bundle are loosely organized, and their plus ends terminate near its midzone, which is enriched for an ill‐defined matrix material. At this time, the distance between the centrosomes is not defined by external forces because these organelles remain stationary when the bundle connecting them is severed by laser microsurgery. However, since the centrosomes move towards one another in response to monastrol treatment, the kinesin‐like motor protein Eg5 is involved. From these results, we conclude that separating asters interact during prometaphase of mitosis to form a spindle‐shaped Mt array, but that in the absence of chromosomes this array is unstable. An analysis of the existing data suggests that the stabilization of spindle Mts during mitosis in vertebrates does not involve the chromatin (i.e., the RCC1/RanGTP pathway), but instead some other chromosomal component, e.g., kinetochores. Cell Motil. Cytoskeleton 52:107–121, 2002. © 2002 Wiley‐Liss, Inc.
Spindle positioning in fibroblasts supports an astral microtubule length dependent force generation at the basal membrane
Authors: Schultz Niklas , Önfelt Agneta 
Published: August 2002

AbstractV79 Chinese hamster fibroblasts that maintain an elongated shape in metaphase occur at a low frequency and often show the spindle asymmetrically positioned. We show here that this aberrant position is corrected in anaphase by an external force, pulling the spindle into place. The force was applied on astral microtubules because spindle motility was hampered when astral microtubules were poorly developed spontaneously, or destroyed by colcemid. Colcemid also abolished the observed downward positioning of centrosomes in anaphase. One pole of the spindle was usually dominant during correction, but occasionally both poles could become subject to pulling making the spindle move perpendicular to the long axis of the cell, which induced reshaping of the cell. The pulling force acted unevenly with short intervals of resting between the pulling. Spindle elongation also varied in rate but showed a different periodicity than translocation of the spindle, and therefore appeared independently regulated. The length of the spindle increased with the length of the cell, and the rate of spindle elongation and pole movement increased with distance moved, indicating that the forces mediated by astral microtubules increase with their length. Arp1/dynactin, not colocalising with tubulin, was more often continuous with microtubules in anaphase B than in metaphase, and was primarily located at the bottom of the cell. Further, shifts in the geometric gravity centre of the cell occurred in the same direction as migration of the spindle. To explain these results, we suggest that astral microtubles transiently anchored at the bottom of the cell are of particular importance for spindle translocation in fibroblasts. Cell Motil. Cytoskeleton 50:69–88, 2001. © 2001 Wiley‐Liss, Inc.
Information transfer on an NMR quantum information processor
Authors: Price Mark D. , Fortunato Evan M. , Pravia Marco A. , Breen Craig , Kumaresean Swami , Rosenberg Gabriel , Cory David G. 
Published: August 2002

AbstractNuclear magnetic resonance (NMR) has been used to explore and demonstrate many of the ideas behind quantum information processing (QIP). This work provides an illustrative example of information transfer over both quantum and classical channels using NMR techniques on the two‐spin compound 13C‐chloroform. A two‐qubit model of a classical channel that is a reduction of a quantum channel can be implemented via the correlation of a spin representing information on a channel, with an ancilla spin representing the surrounding world. When information about this correlation is lost or discarded, the channel becomes classical; otherwise, it remains quantum. It is shown that, while classical information can be transmitted over either a quantum or classical channel, quantum information will only survive transmission over a quantum channel. © 2001 John Wiley & Sons, Inc. Concepts Magn Reson 13: 151–158, 2001
Activation of Src kinase in primary colorectal carcinoma
Authors: Allgayer Heike , Boyd Douglas D. , Heiss Markus M. , Abdalla Eddie K. , Curley Steven A. , Gallick Gary E. 
Published: August 2002

AbstractBACKGROUNDThe specific activity of the non‐receptor protein tyrosine kinase, Src, is increased in the majority of colon and rectal adenocarcinomas compared to normal mucosa. However, the prognostic significance of this difference is unknown. The objective of the current study was to determine if Src activity is a marker for poor clinical prognosis in colon carcinoma patients. As Src activation leads to expression of urokinase/plasminogen activator receptor (u‐PAR), expression of Src and u‐PAR were correlated with patient survival.METHODSTumors and adjacent normal colonic mucosae from 45 patients with colorectal carcinoma were screened for Src activity by the immune complex kinase assay. Expression of u‐PAR was determined by enzyme linked immunoabsorbent assay. The primary tumor‐to‐normal mucosa ratios of activity were compared following classification and regression tree (CART) analysis to determine the prognostic significance of elevated specific Src activity. Expression of u‐PAR was correlated with Src activity.RESULTSBy CART analysis, Src activity in tumors elevated more than twofold over normal mucosa was significant. Increased Src activity significantly correlated with Dukes stage, pT and pN classification, and increased u‐PAR levels (P< 0.001). Kaplan Meier analysis showed a significant association between elevated Src activity and shorter overall survival of all patients (P= 0.0004) and of Dukes Stage A‐C patients (P= 0.0037). In patients who underwent curative resection, a significant correlation with a decreased disease‐free survival rate was found (P< 0.0001). Multivariate analysis revealed that elevated Src activity was a prognostic parameter independent of M classification (P= 0.0125, relative risk 3.54, 95% confidence interval 1.31 – 9.76).CONCLUSIONSSrc activity is an independent indicator of poor clinical prognosis in all stages of human colon carcinoma. These data suggest that Src‐specific inhibitors may have a therapeutic role in inhibiting tumor progression and metastasis, and that measurement of Src activity may aid in selection of early stage patients for adjuvant therapy. Cancer 2002;94:344–51. © 2002 American Cancer Society.
Results with chemotherapy comprised of cyclophosphamide, doxorubicin, vincristine, and prednisone followed by radiotherapy with or without prechemotherapy surgical debulking for patients with bulky, aggressive lymphoma
Authors: Wilder Richard B. , Romaguera Jorge E. , Tucker Susan L. , Ha Chul S. , Hess Mark A. , Cabanillas Fernando , Cox James D. 
Published: August 2002

AbstractBACKGROUNDThe authors performed a case–control analysis of local control, progression free survival, and overall survival in patients with Stage I–II aggressive lymphomas measuring ≥ 7 cm in greatest dimension who were treated initially with or without surgical debulking: All patients then received cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP)‐based chemotherapy followed by involved‐field radiotherapy.METHODSFrom May 1975 through May 1996, 50 patients were treated with (n = 25 patients) or without (n = 25 patients) resection of > 80% of their bulky lymphomas. Chemotherapy consisted of 3–12 cycles of CHOP. In general, patients who underwent debulking received three cycles of chemotherapy, whereas patients who did not undergo debulking received at least six cycles of chemotherapy. The total radiotherapy dose was 40.8 grays (Gy) ± 4.2 Gy (mean ± standard deviation).RESULTSThe median follow‐up was 62 months. Patients who underwent debulking were similar prognostically to patients who did not. There was a trend toward improved local control (5‐year rates: 96% vs. 80%; P = 0.10) and overall survival (5‐year rates: 83% vs. 71%; P = 0.18) in patients who underwent debulking compared with patients who did not, respectively. Progression free survival was significantly better for patients who underwent debulking compared with patients who did not (5‐year rates: 88% vs. 62%, respectively; P = 0.04).CONCLUSIONSBecause this study was retrospective, selection bias may account for the observed difference in progression free survival. Because it is unlikely that a trial randomizing patients with bulky, aggressive lymphoma to surgery will be conducted, the authors' current efforts are focused on escalation of the total radiotherapy dose as a possibly less costly and less morbid approach toward improving progression free survival for these patients. Cancer 2002;94:601–5. © 2002 American Cancer Society.DOI 10.1002/cncr.10260
Cytoplasmic expression of laminin γ2 chain correlates with postoperative hepatic metastasis and poor prognosis in patients with pancreatic ductal adenocarcinoma
Authors: Takahashi Shinichiro , Hasebe Takahiro , Oda Tatsuya , Sasaki Satoshi , Kinoshita Taira , Konishi Masaru , Ochiai Takenori , Ochiai Atsushi 
Published: August 2002

AbstractBACKGROUNDThe laminin γ2 chain is involved in tumor invasion and metastasis, but the significance of laminin γ2 chain expression remains unclear in patients with pancreatic carcinoma.METHODSLaminin γ2 chain expression was examined immunohistochemically in 48 patients with pancreatic ductal adenocarcinoma who were followed closely to elucidate the correlations between clinicopathologic factors, postoperative recurrence, and overall survival. Prognostic factors for postoperative survival were examined comparing clinicopathologic factors and laminin γ2 chain expression.RESULTSTwo different staining patterns of laminin γ2 chain expression, cytoplasmic expression and basement membrane expression, were detected in tumors from all 48 patients. Tumors were then classified into two types according to the dominant pattern of laminin γ2 chain expression: the cytoplasmic expression dominant type (CYT;n= 26 patients) and the basement membrane expression dominant type (BM;n= 22 patients). Tumor differentiation was associated statistically with the BM type of laminin γ2 chain expression (P= 0.0002). The CYT type of laminin γ2 chain expression was associated significantly with the occurrence of postoperative hepatic metastasis (P= 0.0011) and also was the strongest predictive factor for poorer overall survival in patients with pancreatic ductal adenocarcinomas (P= 0.0161).CONCLUSIONSThe cytoplasmic expression of the laminin γ2 chain represents the high invasive potential of the tumor and is correlated with distant metastasis, especially hepatic metastasis, and with a poorer prognosis in patients with pancreatic ductal adenocarcinoma. Cancer 2002;94:1894–901. © 2002 American Cancer Society.DOI 10.1002/cncr.10395