bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircFN1 promotes acute myeloid leukemia cell proliferation and invasion but refrains apoptosis via miR‐1294/ARHGEF10L axis
Authors: Wang Sheng , Zhang Bang‐Shuo , Yang Yi , Fu Lin‐Lin 
Published: January 2024

AbstractPrevious studies have proved circFN1 is highly expressed in acute myeloid leukemia (AML) patients and AML cell lines. This study aims to investigate the impact of circFN1 on AML and its mechanism. Via real‐time quantitative PCR to detect circFN1, miR‐1294, ARHGEF10L expressions in clinical plasma samples and AML cell lines, AML cells were cultured in vitro and transfected with si‐circFN1, pcDNA3.1‐circFN1, and si‐ARHGEF10L, respectively, or co‐transfected pcDNA3.1‐circFN1 + miR‐1294 mimic and pcDNA3.1‐circFN1 + si‐ARHGEF10L. Using dual luciferase reporter experiment to detect the relationship between circFN1 and miR‐1294, as well as miR‐1294 and ARHGEF10L. CCK‐8 was used to detect cell proliferation, Transwell to cell invasion, TUNEL staining and flow cytometry to detect cell apoptosis, RT‐qPCR to circFN1 RNA, miR‐1294, and ARHGEF10L expression levels in HL‐60 cells, and western blot to ARHGEF10L protein expression level in HL‐60 cells. We found highly expressed circFN1 and ARHGEF10L, as well as low‐expressed miR‐1294 in AML patients and AML cell lines. In contrast to si‐NC group, si‐circFN1 group could signally inhibit HL‐60 cell proliferation and migration, but promote cell apoptosis; compared with mimic NC group, miR‐1294 mimic group could visually inhibit HL‐60 cell proliferation and migration, but promote cell apoptosis. miR‐1294 was the target of circFN1, and ARHGEF10L was the target of miR‐1294. Over‐expressing miR‐1294 or silencing ARHGEF10L could signally inhibit circFN1 promoting HL‐60 cell proliferation and migration and repressing cell apoptosis. circFN1 promotes proliferation and invasion of AML cell and represses cell apoptosis via regulating miR‐1294/ARHGEF10L axis, which provides new insight for molecular targeted‐treatment for AML.
A regime shift between soil moisture and temperature in the 1950s over the permafrost region of Northeast China
Authors: Li Qiang , Liu Yu , Wang Lu , Pang Wenxuan , Zhang Xuechen , Xu Ling , Song Huiming , Guo Wei , Sun Changfeng , Cai Qiufang , Ren Meng , Cheng Hai 
Published: January 2024

AbstractPermafrost is one of the essential carbon pools in the world. Due to limited studies on historical soil moisture changes and the coupling relationship between soil moisture and temperature in permafrost regions, significant uncertainty exists in the carbon loss in permafrost predicted by different models under global warming scenarios. Based on the tree‐ring width chronology of Pinus sylvestris var. mongholica Litv. growing in the southern edge of the Eurasian continuous permafrost zone, we reconstructed the summer (June–September) 0–1 m soil moisture variations from 1705 to 2009, which could explain 45.6% of the variance in the observed soil moisture. Overall, local precipitation and temperature exhibited statistically significant positive feedback (p < 0.001) to soil moisture before the 1950s, indicating that the warm/humid climate pattern was conducive to soil moisture conservation before the Anthropocene Epoch. However, the effect of temperature on soil moisture has shifted suddenly to negative since the 1950s, implying that the positive soil moisture‐temperature relationship during the past three centuries has been disrupted by the unprecedented warming in the Modern Warm Period. Furthermore, we found that the temporal relationship of the soil moisture‐temperature (15‐year sliding correlation) in the study area is negatively regulated by the global mean temperature variations (p < 0.01). The regime shift between soil moisture and temperature might be attributed to the superimposed influence of natural and anthropogenic factors since the 1950s. Although the warming leads to the melting of the permafrost layer, and thus the increase in soil moisture content, the enhanced evapotranspiration caused by warming up results in more water loss and drier soil. This study provides historical evidence of shifted soil moisture‐temperature coupling in the permafrost zone, warning that soil moisture in the permafrost region may further decline under global warming scenarios, thereby affecting vegetation growth and forest carbon sequestration potential.
Mechanical behavior of the polyamide 6.6 nanofiber and MWCNT‐reinforced hybrid nanocomposites
Authors: Gunoz Alper , Kara Memduh 
Published: January 2024

AbstractThe present study examines the influence of polyamide 6.6 (PA 6.6) nanofiber and MWCNTs reinforcements on the mechanical behavior of nanocomposite plates formed by hybridization of carbon fabric (CF) and kevlar fabric (KF). First, PA 6.6 nanofibers were manufactured by electrospinning. The produced nanofibers were placed in the interlaminar of nanocomposite plates. PA 6.6 nanofiber and MWCNT‐reinforced nanocomposite plates consisting of 12 layers were produced by the vacuum bagging method. Tensile, Mode‐II fracture and bending tests were applied to the produced composite plates in accordance with the standards. After the mechanical tests, the nanocomposite plates' macro and micro damage analysis was performed. As a result of the reinforcement of PA 6.6, MWCNTs and PA 6.6‐MWCNTs, the tensile strength, interlaminar fracture toughness and bending strength of the nanocomposite plates significantly enhanced compared to unreinforced samples. In addition, interlaminar crack propagation in reinforced samples became more stable than in the unreinforced sample.Highlights
Investigated the impact of PA 6.6 nanofiber and MWCNT reinforcements on nanocomposite plates formed through CF and KF hybridization.
Reinforcement with PA 6.6, MWCNTs, and PA 6.6‐MWCNTs significantly improved tensile strength, interlaminar fracture toughness, and bending strength.
Mode‐II interlaminar fracture toughness tests demonstrated enhanced crack propagation stability with PA 6.6 nanofiber and MWCNT reinforcements.
Registration of ‘Espresso’ lowland switchgrass
Authors: Rushing J. Brett , Baldwin Brian S. , Morrison Jesse I. 
Published: January 2024

Abstract‘Espresso’ (Reg. no. CV‐293, PI 687202) is a lowland ecotype (Gulf sub‐population) of switchgrass (Panicum virgatum L.) developed and released by Mississippi State University and the Mississippi Agricultural and Forestry Experiment Station. Espresso was developed from seven cycles of phenotypic recurrent selection for rapid seed germination without stratification. All cycles of selection were made using seed harvested from open‐pollinated isolation blocks containing approximately 100 mother plants. Germination was assessed in laboratory conditions for 15 years, culminating in 2 years of field‐based germination and emergence evaluation at five locations across Mississippi. Over a 2‐year period, germination and emergence of Espresso was significantly greater (P < 0.05) than seed from ‘Alamo’ and ‘Kanlow’. Field testing revealed Espresso as having significantly (P < 0.05) greater establishment success (mean number of seedlings per linear 30 cm) when compared to Alamo. Espresso was released based on the need for southeastern US‐adapted germplasm that possesses enhanced germination characteristics.
Registration of ‘FSA1602’ St. Augustinegrass
Authors: Kenworthy Kevin , Quesenberry Kenneth , Aldrich Kendall , Buhlman Jamie , Rios Esteban , Unruh Bryan , Harmon Philip , Dale Adam , Milla‐Lewis Susana , Schwartz Brian , Raymer Paul , Chandra Ambika , Wu Yanqi , Zhang Jing , Wherley Ben , Martin Dennis , Moss Justin , Fontainier Charles , Miller Grady 
Published: January 2024

Abstract‘FSA1602’ (Reg. no. CV‐294, PI 704119) hybrid St. Augustinegrass [Stenotaphrum secundatum (Walter) Kuntze] was developed and released by the Florida Agricultural Experiment Station, University of Florida, in 2018. FSA1602 has a distinct olive blue‐green color and high levels of resistance to gray leaf spot, take‐all root rot, and excellent shade tolerance and turfgrass quality (TQ). It is targeted for use in residential and commercial lawns in the southern United States. FSA1602 has coarse textured leaves similar in width to ‘Floratam’ and leaf lengths similar to ‘SS‐100’ (Palmetto) but shorter than Floratam. FSA1602 stolon width is larger than Floratam or Palmetto but has a mean stolon internode length shorter than either Floratam or Palmetto. It produces a dense turfgrass with high TQ that is similar to or better than Floratam and with less winter kill than Floratam, which is the most widely used St. Augustinegrass for lawns in Florida.
A conserved but structurally divergent loop in acyl protein thioesterase 1 regulates its catalytic activity, ligand binding, and folded stability
Authors: Harris William Trey , Altieri Isabelle , Gieck Isabella , Johnson R. Jeremy 
Published: January 2024

AbstractHuman acyl protein thioesterases (APTs) catalyze the depalmitoylation of S‐acylated proteins attached to the plasma membrane, facilitating reversible cycles of membrane anchoring and detachment. We previously showed that a bacterial APT homologue, FTT258 from the gram‐negative pathogen Francisella tularensis, exists in equilibrium between a closed and open state based on the structural dynamics of a flexible loop overlapping its active site. Although the structural dynamics of this loop are not conserved in human APTs, the amino acid sequence of this loop is highly conserved, indicating essential but divergent functions for this loop in human APTs. Herein, we investigated the role of this loop in regulating the catalytic activity, ligand binding, and protein folding of human APT1, a depalmitoylase connected with cancer, immune, and neurological signaling. Using a combination of substitutional analysis with kinetic, structural, and biophysical characterization, we show that even in its divergent structural location in human APT1 that this loop still regulates the catalytic activity of APT1 through contributions to ligand binding and substrate positioning. We confirmed previously known roles for multiple residues (Phe72 and Ile74) in substrate binding and catalysis while adding new roles in substrate selectivity (Pro69), in catalytic stabilization (Asp73 and Ile75), and in transitioning between the membrane binding β‐tongue and substrate‐binding loops (Trp71). Even conservative substitution of this tryptophan (Trp71) fulcrum led to complete loss of catalytic activity, a 13°C decrease in total protein stability, and drastic drops in ligand affinity, indicating that the combination of the size, shape, and aromaticity of Trp71 are essential to the proper structure of APT1. Mixing buried hydrophobic surface area with contributions to an exposed secondary surface pocket, Trp71 represents a previously unidentified class of essential tryptophans within α/β hydrolase structure and a potential allosteric binding site within human APTs.
Inhibition of glycolysis and SIRT1/GLUT1 signaling ameliorates the apoptotic effect of Leptosidin in prostate cancer cells
Authors: Park Youngsang , Lee Hyo‐Jung , Sim Deok Yong , Park Ji Eon , Ahn Chi‐Hoon , Park Su‐Yeon , Lee Yu‐Chan , Shim Bum‐Sang , Kim Bonglee , Kim Sung‐Hoon 
Published: January 2024

AbstractSince the silent information regulation 2 homolog‐1 (sirtuin, SIRT1) and glucose transporter 1 (GLUT1) are known to modulate cancer cell metabolism and proliferation, the role of SIRT1/GLUT1 signaling was investigated in the apoptotic effect of Leptosidin from Coreopsis grandiflora in DU145 and PC3 human prostate cancer (PCa) cells. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, cell cycle analysis, Western blotting, cBioportal correlation analysis, and co‐immunoprecipitation were used in this work. Leptosidin showed cytotoxicity, augmented sub‐G1 population, and abrogated the expression of pro‐poly (ADP‐ribose) polymerase (pro‐PARP) and pro‐cysteine aspartyl‐specific protease (pro‐caspase3) in DU145 and PC3 cells. Also, Leptosidin inhibited the expression of SIRT1, GLUT1, pyruvate kinase isozymes M2 (PKM2), Hexokinase 2 (HK2), and lactate dehydrogenase A (LDHA) in DU145 and PC3 cells along with disrupted binding of SIRT1 and GLUT1. Consistently, Leptosidin curtailed lactate, glucose, and ATP in DU145 and PC3 cells. Furthermore, SIRT1 depletion enhanced the decrease of GLUT1, LDHA, and pro‐Cas3 by Leptosidin in treated DU145 cells, while pyruvate suppressed the ability of Leptosidin in DU145 cells. These findings suggest that Leptosidin induces apoptosis via inhibition of glycolysis and SIRT1/GLUT1 signaling axis in PCa cells.
Post‐traumatic pleural effusion: Don't forget the chylothorax!
Authors: Bovy Valentin , De Keukeleire Tom , Van Schoote Elke , Vanslembrouck Inge , Bogaert Anne‐Marie 
Published: January 2024

AbstractIn this case we describe a 58‐year‐old male with bilateral pleural effusion after a blunt trauma to the back. A pleural puncture revealed a chylothorax. An additional computed tomography scan showed a vertebral fracture at level D8 with rupture of the nearby thoracic duct. Our patient could be treated with a conservative approach. This case highlights the importance of ruling out a chylothorax in any post‐traumatic pleural effusion. Despite the low prevalence, we consider it a don't‐miss diagnosis given the specific treatment requirements.
Farming of ZnO−SnO2 Nanocubes for Chemiresistive NO2 Gas Detection
Authors: Dhage Swapnali B. , Patil Vithoba L. , Yelpale Arvind M. , Malghe Yuvraj S. 
Published: January 2024

AbstractThe ZnO−SnO2 nanocomposite has been synthesized using the simple and low‐cost co‐precipitation method. The physicochemical characterization of the prepared nanocomposites was carried out by using X‐ray diffraction (XRD), Field Emission Scanning Electron Microscope (FE‐SEM), High‐resolution Transmission Electron Microscopy (HR‐TEM), Energy Dispersive X‐ray analysis (EDAX) and UV‐visible spectroscopy techniques. In XRD confirmed that, hexagonal wurtzite phase of ZnO with the tetragonal rutile phase of SnO2 was found to be in the pure form. The particle size of the ZnO−SnO2 nanocomposite was fund to be ~23 nm in size. The nano cubes like surface morphology of the ZnO−SnO2 nanocomposite has been investigated using FESEM. Based on this various characterization, the ZnO−SnO2 nanocomposite has been used for the development of the chemiresistive gas sensor application. The gas sensitivity is found to be 29.30 % for 100 ppm NO2 gas at 200 °C operating temperature. This work has been used for the development of gas sensor devices for practical application.
Underwater Bionic Self‐Healing Superhydrophobic Coating with the Synergetic Effect Of Hydrogen Bonds and Self‐Formed Bubbles
Authors: Li Hao , Xin Lei , Gao Jian , Shao Yanlong , Zhang Zhihui , Ren Luquan 
Published: January 2024

AbstractThe self‐healing ability of superhydrophobic surfaces in air has attracted tremendous additions in recent years. Once the superhydrophobic surface is damaged underwater, water seeps into gaps among micro/nano structures. The air film diffuses into water and eventually disappears during immersion without actively replenishing the gas, which results in the impossible of self‐healing. Here, an underwater self‐healing superhydrophobic coating with the synergetic effect of hydrogen bonds and self‐formed bubbles via the spraying method is fabricated. The movement of hydrogen bonds of the prepared polyurethane enables microstructures to reconstruct at room temperature and self‐formed bubbles of effervescent materials underwater actively replenish gas before microstructures completely self‐healing, achieving the self‐healing property of the superhydrophobic coating. Moreover, the hydrophilic effervescent material is sprayed along with unmodified micron‐scaled particles because modified nano‐scale particles are key factors for the realization of superhydrophobic coating. An underwater stable superhydrophobic surface with pressure resistance (4.9 kPa) is demonstrated. This superhydrophobic coating also shows excellent drag reduction, anti‐icing, and anti‐corrosion properties. This facile and scalable method offers a new route that an underwater self‐healing superhydrophobic coating executes the gas film recovery.
Multifaceted application of modified biochar for water and wastewater treatment
Authors: Mohit Aggarwal , Remya Neelancherry , Priyadarshini Upasana 
Published: January 2024

AbstractBiochar, a carbon‐rich material produced through the pyrolysis of biomass, has been shown to have the potential to remove contaminants from water and wastewater. However, due to the weak adsorption capacity and narrow adsorption range of pure biochar, it is modified, either chemically or physically, in order to optimize its applications in water and wastewater treatment. This study summarizes the recent advancements in the application of modified biochar for water and wastewater treatment. Modification of biochar increases its surface area and porous structure, which makes it an effective adsorbent to immobilize a range of contaminants, such as heavy metals, dyes, and organic contaminants. It can also be used as a catalyst in the biodegradation of organic pollutants as it can provide a substrate for microbial growth and metabolism, enhancing pollutant degradation. This study also summarizes various biochar modifications such as gas, acid, alkali, metal, organic compounds, and oxidizing agents. Among the metal‐modified biochar, Iron‐modified biochar has shown the highest tetracycline (TC) removal efficiency (90.7%) and nitrate adsorption capacity (32.33 mg g−1). N‐doped biochars were found to be the most effective biochars for removing heavy metals such as Cd2+ or Cu2+ and aromatic compounds as they can increase the adsorption of raw biochar by up to 70%. Increasing the number of functional groups on the surface of the biochar by using organic compounds like acrylonitrile and chitosan showed a two to three‐fold increase in the adsorption capacity of pollutants such as Cd2+ and Hg2+. According to this review, modified biochar technology is a cost‐effective and environmentally friendly wastewater treatment method.
Metal levels in Sander lucioperca (Linnaeus, 1758) and their health risk assessment for consumers
Authors: Bat Levent , Yardım Öztekin , Öztekin Ayşah 
Published: January 2024

AbstractThe current study was conducted to determine the residues of Zn, Fe, Cu, Cd, Hg, Pb, and As in the edible tissues of Sander lucioperca. Total of 20 fish individuals collected from Lake Balık, Samsun were analysed by using ICPMS (Inductively Coupled Plasma Mass Spectrophotometer). All metal values in the muscle of S. lucioperca were quite low. Fe is the highest concentration (4.07 mg/kg), whereas Cd had the lowest Cd (0.007 mg/kg). Therefore, it can be said that the bioaccumulation of metals in pike‐perch did not go over the acceptable limits established for these metals by national and international organisations. The hazard quotients (HQs) for individual elements and the hazard index (HI) were all <1 in S. lucioperca, showing no danger by these chemicals when consumed by infants, children, and adults. The RIs for Pb were between 1.45 × 10−7 and 4.30 × 10−7, indicating their insignificant carcinogenic risks, whereas for As these values were between 2.78 × 10−5 and 1.06 × 10−4, indicating their tolerable or acceptable carcinogenic risks.
Participant completion of longitudinal assessments in an online cognitive aging registry: The role of medical conditions
Authors: Ashford Miriam T. , Jin Chengshi , Neuhaus John , Diaz Adam , Aaronson Anna , Tank Rachana , Eichenbaum Joseph , Camacho Monica R. , Fockler Juliet , Ulbricht Aaron , Flenniken Derek , Truran Diana , Mackin Robert Scott , Weiner Michael W. , Mindt Monica Rivera , Nosheny Rachel L. 
Published: January 2024

AbstractINTRODUCTIONThis study aimed to understand whether older adults’ longitudinal completion of assessments in an online Alzheimer's disease and related dementias (ADRD)–related registry is influenced by self‐reported medical conditions.METHODSBrain Health Registry (BHR) is an online cognitive aging and ADRD‐related research registry that includes longitudinal health and cognitive assessments. Using logistic regressions, we examined associations between longitudinal registry completion outcomes and self‐reported (1) number of medical conditions and (2) eight defined medical condition groups (cardiovascular, metabolic, immune system, ADRD, current psychiatric, substance use/abuse, acquired, other specified conditions) in adults aged 55+ (N = 23,888). Longitudinal registry completion outcomes were assessed by the completion of the BHR initial questionnaire (first questionnaire participants see at each visit) at least twice and completion of a cognitive assessment (Cogstate Brief Battery) at least twice. Models included ethnocultural identity, education, age, and subjective memory concern as covariates.RESULTSWe found that the likelihood of longitudinally completing the initial questionnaire was negatively associated with reporting a diagnosis of ADRD and current psychiatric conditions but was positively associated with reporting substance use/abuse and acquired medical conditions. The likelihood of longitudinally completing the cognitive assessment task was negatively associated with number of reported medical conditions, as well as with reporting cardiovascular conditions, ADRD, and current psychiatric conditions. Previously identified associations between ethnocultural identity and longitudinal assessment completion in BHR remained after accounting for the presence of medical conditions.DISCUSSIONThis post hoc analysis provides novel, initial evidence that older adults’ completion of longitudinal assessments in an online registry is associated with the number and types of participant‐reported medical conditions. Our findings can inform future efforts to make online studies with longitudinal health and cognitive assessments more usable for older adults with medical conditions. The results need to be interpreted with caution due to selection biases, and the under‐inclusion of minoritized communities.
The assessment and management of cirrhotic patients with encephalopathy
Authors: Kibble H. , Shawcross Debbie L. 
Published: January 2024

AbstractHepatic encephalopathy (HE) is a debilitating complication associated with both acute and chronic liver injury. It is associated with a greater risk of death than any other significant hepatic decompensation event. It manifests as a wide spectrum of neuropsychological abnormalities ranging from subtle impairments in higher cognitive function, to confusion and coma. The pathophysiological role of ammonia in the development of HE is well known, but there is increasing recognition that the gut microbiome, gut‐derived systemic inflammation and development of infection can serve as drivers of HE in patients with cirrhosis. The development of HE portents to the severity of cirrhosis and the prognosis is poor without liver transplantation. A referral for liver transplantation should therefore be considered early in those who are eligible. This review covers the pragmatic assessment of HE in patients with cirrhosis, as well as the current evidence base for the best practice management of HE in such patients.
Industrieschnee: An overview of some European anthropogenic snowfall events, with a case study from Bern (Switzerland)
Authors: Graham Edward 
Published: January 2024

AbstractIndustrieschnee or anthropogenic snowfall is a local winter hazard in many European cities and towns under suitable anticyclonic conditions and in the vicinity of persistent local water vapour sources and air pollution. Here, we overview the formation of Industrieschnee and describe a case study event from Bern, Switzerland, comparing the estimated mass of snow deposited with the water vapour emissions from a local incinerator. We speculate that previous European Industrieschnee events may have been considerably under‐reported, or falsely attributed to surface hoar frost (reif) or the accretion of rime (raureif).