bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of deoxidation process for off-grade titanium sponge using magnesium metal with wire mesh strainer type of crucible
Authors: Park Sung-Hun , Jeoung Hyeong-Jun , Lee Tae-Hyuk , Sohn Ho-Sang , Kang Jungshin 
Published: January 2024

AbstractIn this study, the deoxidation process for off-grade titanium (Ti) sponge using magnesium (Mg) metal with a wire mesh strainer type of crucible was developed. Ti hydride (TiH2) feedstock, which was prepared by hydrogenating off-grade Ti sponge, was deoxidized using Mg in a molten magnesium chloride–potassium chloride salt at 933 K under an argon and 20% hydrogen (H2) mixed gas atmosphere. After deoxidation, the residual Mg-containing salt was separated in situ from the crucible to investigate the feasibility of minimizing salt loss during the leaching and production of pure TiH2. The results showed that the presence of residual Mg-containing salt inside the crucible strongly influenced whether a mixture of Ti and TiH2 or pure TiH2 was produced. When the salt was not sufficiently separated, a mixture of Ti and TiH2 was obtained and its oxygen (O) concentration was 0.121 mass% under certain conditions. Meanwhile, pure TiH2 was obtained by increasing the H2 gas flow rate during deoxidation. Therefore, these results demonstrate that the decrease of O concentration to below 0.180 mass% and the minimal loss of the salt are feasible.
Renal denervation restores biomechanics of carotid arteries in a rat model of hypertension
Authors: Gkousioudi Anastasia , Razzoli Margherita , Moreira Jesse D. , Wainford Richard D. , Zhang Yanhang 
Published: January 2024

AbstractThe prevalence of hypertension increases with aging and is associated with increased arterial stiffness. Resistant hypertension is presented when drug treatments fail to regulate a sustained increased blood pressure. Given that the mechanisms between the sympathetic nervous system and the kidney play an important role in blood regulation, renal denervation (RDN) has emerged as a therapeutic potential in resistant hypertension. In this study, we investigated the effects of RDN on the biomechanical response and microstructure of elastic arteries. Common carotid arteries (CCA) excised from 3-month, 8-month, and 8-month denervated rats were subjected to biaxial extension-inflation test. Our results showed that hypertension developed in the 8-month-old rats. The sustained elevated blood pressure resulted in arterial remodeling which was manifested as a significant stress increase in both axial and circumferential directions after 8 months. RDN had a favorable impact on CCAs with a restoration of stresses in values similar to control arteries at 3 months. After biomechanical testing, arteries were imaged under a multi-photon microscope to identify microstructural changes in extracellular matrix (ECM). Quantification of multi-photon images showed no significant alterations of the main ECM components, elastic and collagen fibers, indicating that arteries remained intact after RDN. Regardless of the experimental group, our microstructural analysis of the multi-photon images revealed that reorientation of the collagen fibers might be the main microstructural mechanism taking place during pressurization with their straightening happening during axial stretching.
LDL cholesterol target attainment in cardiovascular high- and very-high-risk patients with statin intolerance: a simulation study
Authors: Katzmann Julius L. , Stürzebecher Paulina E. , Kruppert Silvia , Laufs Ulrich 
Published: January 2024

AbstractThe inability to tolerate sufficient doses of statins, statin intolerance (SI), contributes to the non-achievement of guideline-recommended low-density lipoprotein cholesterol (LDL-C) treatment targets. Patients with SI require alternative lipid-lowering therapies (LLT). We conducted a simulation study on LDL-C target achievement with oral LLT (ezetimibe, bempedoic acid) in patients with SI, using representative data of 2.06 million German outpatients. SI was defined using literature-informed definitions based on electronic medical records (EMR). Among n = 130,778 patients with hypercholesterolaemia, available LDL-C measurement, and high or very-high cardiovascular risk, 8.6% met the definition of SI. Among patients with SI, 7.7% achieved the LDL-C target at baseline. After simulation of the stepwise addition of treatment with ezetimibe and bempedoic acid, 22.6 and 52.0% achieved the LDL-C target, respectively. The median achieved LDL-C was 80 and 62 mg/dL, the corresponding reductions from baseline were 20.0 and 38.0%, respectively. A higher proportion of patients classified as high risk achieved the target compared to those at very-high risk (58.1 vs. 49.9%). In conclusion, in patients with increased cardiovascular risk meeting the definition of SI based on EMR, combination LLT with ezetimibe and bempedoic acid has the potential to substantially increase the proportion of patients achieving clinically relevant LDL-C reductions.
Cell migration, DNA fragmentation and antibacterial properties of novel silver doped calcium polyphosphate nanoparticles
Authors: Ekram Basma , Tolba Emad , El-Sayed Ahmed F. , Müller Werner E. G. , Schröder Heinz C. , Wang Xiaohong , Abdel-Hady Bothaina M. 
Published: January 2024

AbstractTo combat infections, silver was used extensively in biomedical field but there was a need for a capping agent to eliminate its cytotoxic effects. In this study, polymeric calcium polyphosphate was doped by silver with three concentrations 1, 3 or 5 mol.% and were characterized by TEM, XRD, FTIR, TGA. Moreover, cytotoxicity, antibacterial, cell migration and DNA fragmentation assays were done to assure its safety. The results showed that the increase in silver percentage caused an increase in particle size. XRD showed the silver peaks, which indicated that it is present in its metallic form. The TGA showed that thermal stability was increased by increasing silver content. The antibacterial tests showed that the prepared nanoparticles have an antibacterial activity against tested pathogens. In addition, the cytotoxicity results showed that the samples exhibited non-cytotoxic behavior even with the highest doping concentration (5% Ag-CaPp). The cell migration assay showed that the increase in the silver concentration enhances cell migration up to 3% Ag-CaPp. The DNA fragmentation test revealed that all the prepared nanoparticles caused no fragmentation. From the results we can deduce that 3% Ag-CaPp was the optimum silver doped calcium polyphosphate concentration that could be used safely for medical applications.
High-performance spinel NiMn2O4 supported carbon felt for effective electrochemical conversion of ethylene glycol and hydrogen evolution applications
Authors: Medany Shymaa S. , Hefnawy Mahmoud A. , Kamal Soha M. 
Published: January 2024

AbstractOne of the most effective electrocatalysts for electrochemical oxidation reactions is NiMn2O4 spinel oxide. Here, a 3-D porous substrate with good conductivity called carbon felt (CF) is utilized. The composite of NiMn2O4-supported carbon felt was prepared using the facile hydrothermal method. The prepared electrode was characterized by various surface and bulk analyses like powder X-ray diffraction, X-ray photon spectroscopy (XPS), Scanning and transmitted electron microscopy, thermal analysis (DTA), energy dispersive X-ray (EDX), and Brunauer–Emmett–Teller (BET). The activity of NiMn2O4 toward the electrochemical conversion of ethylene glycol at a wide range of concentrations was investigated. The electrode showed a current density of 24 mA cm−2 at a potential of 0.5 V (vs. Ag/AgCl). Furthermore, the ability of the electrode toward hydrogen evaluation in an alkaline medium was performed. Thus, the electrode achieved a current density equal 10 mA cm−2 at an overpotential of 210 mV (vs. RHE), and the provided Tafel slope was 98 mV dec−1.
Recombination-aware phylogenetic analysis sheds light on the evolutionary origin of SARS-CoV-2
Authors: Esquivel Gomez Luis Roger , Weber Ariane , Kocher Arthur , Kühnert Denise 
Published: January 2024

AbstractSARS-CoV-2 can infect human cells through the recognition of the human angiotensin-converting enzyme 2 receptor. This affinity is given by six amino acid residues located in the variable loop of the receptor binding domain (RBD) within the Spike protein. Genetic recombination involving bat and pangolin Sarbecoviruses, and natural selection have been proposed as possible explanations for the acquisition of the variable loop and these amino acid residues. In this study we employed Bayesian phylogenetics to jointly reconstruct the phylogeny of the RBD among human, bat and pangolin Sarbecoviruses and detect recombination events affecting this region of the genome. A recombination event involving RaTG13, the closest relative of SARS-CoV-2 that lacks five of the six residues, and an unsampled Sarbecovirus lineage was detected. This result suggests that the variable loop of the RBD didn’t have a recombinant origin and the key amino acid residues were likely present in the common ancestor of SARS-CoV-2 and RaTG13, with the latter losing five of them probably as the result of recombination.
An N-glycome tissue atlas of 15 human normal and cancer tissue types determined by MALDI-imaging mass spectrometry
Authors: Wallace Elizabeth N. , West Connor A. , McDowell Colin T. , Lu Xiaowei , Bruner Evelyn , Mehta Anand S. , Aoki-Kinoshita Kiyoko F. , Angel Peggi M. , Drake Richard R. 
Published: January 2024

AbstractN-glycosylation is an abundant post-translational modification of most cell-surface proteins. N-glycans play a crucial role in cellular functions like protein folding, protein localization, cell–cell signaling, and immune detection. As different tissue types display different N-glycan profiles, changes in N-glycan compositions occur in tissue-specific ways with development of disease, like cancer. However, no comparative atlas resource exists for documenting N-glycome alterations across various human tissue types, particularly comparing normal and cancerous tissues. In order to study a broad range of human tissue N-glycomes, N-glycan targeted MALDI imaging mass spectrometry was applied to custom formalin-fixed paraffin-embedded tissue microarrays. These encompassed fifteen human tissue types including bladder, breast, cervix, colon, esophagus, gastric, kidney, liver, lung, pancreas, prostate, sarcoma, skin, thyroid, and uterus. Each array contained both normal and tumor cores from the same pathology block, selected by a pathologist, allowing more in-depth comparisons of the N-glycome differences between tumor and normal and across tissue types. Using established MALDI-IMS workflows and existing N-glycan databases, the N-glycans present in each tissue core were spatially profiled and peak intensity data compiled for comparative analyses. Further structural information was determined for core fucosylation using endoglycosidase F3, and differentiation of sialic acid linkages through stabilization chemistry. Glycan structural differences across the tissue types were compared for oligomannose levels, branching complexity, presence of bisecting N-acetylglucosamine, fucosylation, and sialylation. Collectively, our research identified the N-glycans that were significantly increased and/or decreased in relative abundance in cancer for each tissue type. This study offers valuable information on a wide scale for both normal and cancerous tissues, serving as a reference for future studies and potential diagnostic applications of MALDI-IMS.
ADAMTS13 regulates angiogenic markers via Ephrin/Eph signaling in human mesenchymal stem cells under serum-deprivation stress
Authors: Dutta Gupta Srishti , Ta Malancha 
Published: January 2024

AbstractMesenchymal stem cells (MSCs) are known to facilitate angiogenesis and promote neo-vascularization via secretion of trophic factors. Here, we explored the molecular mechanism adopted by ADAMTS13 in modulating the expression of some key angiogenic markers in human umbilical cord-derived MSCs under serum-deprivation stress. Wharton’s jelly MSCs (WJ-MSCs) were isolated from the perivascular region of human umbilical cords by explant culture. ADAMTS13 was upregulated at both mRNA and protein levels in WJ-MSCs under serum-deprivation stress. Correspondingly, some key angiogenic markers were also seen to be upregulated. By screening signaling pathways, p38 and JNK pathways were identified as negative and positive regulators for expression of ADAMTS13, and the angiogenic markers, respectively. Our results also indicated the Notch pathway and p53 as other probable partners modulating the expression of ADAMTS13 and the angiogenic markers. Knockdown of ADAMTS13 using siRNA led to reversal in the expression of these angiogenic markers. Further, ADAMTS13 was shown to act via the EphrinB2/EphB4 axis followed by ERK signaling to control expression of the angiogenic markers. Interestingly, stronger expression levels were noted for ADAMTS13, VEGF and PDGF under a more stringent nutrient stress condition. Thus, we highlight a novel role of ADAMTS13 in WJ-MSCs under nutrient stress condition.
Research on fire early warning index system of coal mine goaf based on multi-parameter fusion
Authors: Wang Beifang , Lv Yuanhao , Liu Chunbao 
Published: January 2024

AbstractIn order to improve the precision of goaf fire early warning outcomes, this paper obtains the temperature characteristic index of goaf fire early warning by using a coal spontaneous combustion thermogravimetric test and a coal spontaneous combustion programmed heating test. The major gas index and auxiliary gas index for early warning are derived using the integration of the Graham coefficient and grey correlation approach. The D-S evidence theory, which involves optimizing weight allocation, is utilized to integrate the early warning temperature index and various gas indexes. Based on the fusion results, a coal mine goaf fire early warning index system is developed through multi-parameter fusion. The early warning index system is then validated through a programmed heating experiment. The results show that the process of coal spontaneous combustion can be categorized into six distinct stages: latent stage, oxidation stage, critical stage, pyrolysis stage, fission stage, and combustion stage. These stages are determined by the characteristic temperatures of coal spontaneous combustion, which are 31.7 °C, 54.8 °C, 153.7 °C, 204.5 °C, and 241.6 °C. The major gas index for early warning of goaf fires can be determined by 100∆(CO)/∆O2(%). Additionally, auxiliary gas indexes such as C3H8/CH4, C3H8/C2H6, C2H4/C2H6, and C2H2 can be used for goaf fire early warning. The programmed heating experiment shows that the early warning system software is designed by the multi-parameter fusion goaf fire early warning index system is accurate and effective. The selection of the goaf fire early warning index is more rational and precise when using the multi-parameter fusion goaf fire early warning index system based on the D-S evidence theory of weight allocation. It offers robust support for enhancing the goaf fire early warning index system and predicting coal mine goaf fires.
Extensive antibody search with whole spectrum black-box optimization
Authors: Tučs Andrejs , Ito Tomoyuki , Kurumida Yoichi , Kawada Sakiya , Nakazawa Hikaru , Saito Yutaka , Umetsu Mitsuo , Tsuda Koji 
Published: January 2024

AbstractIn designing functional biological sequences with machine learning, the activity predictor tends to be inaccurate due to shortage of data. Top ranked sequences are thus unlikely to contain effective ones. This paper proposes to take prediction stability into account to provide domain experts with a reasonable list of sequences to choose from. In our approach, multiple prediction models are trained by subsampling the training set and the multi-objective optimization problem, where one objective is the average activity and the other is the standard deviation, is solved. The Pareto front represents a list of sequences with the whole spectrum of activity and stability. Using this method, we designed VHH (Variable domain of Heavy chain of Heavy chain) antibodies based on the dataset obtained from deep mutational screening. To solve multi-objective optimization, we employed our sequence design software MOQA that uses quantum annealing. By applying several selection criteria to 19,778 designed sequences, five sequences were selected for wet-lab validation. One sequence, 16 mutations away from the closest training sequence, was successfully expressed and found to possess desired binding specificity. Our whole spectrum approach provides a balanced way of dealing with the prediction uncertainty, and can possibly be applied to extensive search of functional sequences.
Immune, metabolic landscapes of prognostic signatures for lung adenocarcinoma based on a novel deep learning framework
Authors: Qin Shimei , Sun Shibin , Wang Yahui , Li Chao , Fu Lei , Wu Ming , Yan Jinxing , Li Wan , Lv Junjie , Chen Lina 
Published: January 2024

AbstractLung adenocarcinoma (LUAD) is a malignant tumor with high lethality, and the aim of this study was to identify promising biomarkers for LUAD. Using the TCGA-LUAD dataset as a discovery cohort, a novel joint framework VAEjMLP based on variational autoencoder (VAE) and multilayer perceptron (MLP) was proposed. And the Shapley Additive Explanations (SHAP) method was introduced to evaluate the contribution of feature genes to the classification decision, which helped us to develop a biologically meaningful biomarker potential scoring algorithm. Nineteen potential biomarkers for LUAD were identified, which were involved in the regulation of immune and metabolic functions in LUAD. A prognostic risk model for LUAD was constructed by the biomarkers HLA-DRB1, SCGB1A1, and HLA-DRB5 screened by Cox regression analysis, dividing the patients into high-risk and low-risk groups. The prognostic risk model was validated with external datasets. The low-risk group was characterized by enrichment of immune pathways and higher immune infiltration compared to the high-risk group. While, the high-risk group was accompanied by an increase in metabolic pathway activity. There were significant differences between the high- and low-risk groups in metabolic reprogramming of aerobic glycolysis, amino acids, and lipids, as well as in angiogenic activity, epithelial-mesenchymal transition, tumorigenic cytokines, and inflammatory response. Furthermore, high-risk patients were more sensitive to Afatinib, Gefitinib, and Gemcitabine as predicted by the pRRophetic algorithm. This study provides prognostic signatures capable of revealing the immune and metabolic landscapes for LUAD, and may shed light on the identification of other cancer biomarkers.
Improving counseling effectiveness with virtual counselors through nonverbal compassion involving eye contact, facial mimicry, and head-nodding
Authors: Choi Doo Sung , Park Jongyoul , Loeser Martin , Seo Kyoungwon 
Published: January 2024

AbstractAn effective way to reduce emotional distress is by sharing negative emotions with others. This is why counseling with a virtual counselor is an emerging methodology, where the sharer can consult freely anytime and anywhere without having to fear being judged. To improve counseling effectiveness, most studies so far have focused on designing verbal compassion for virtual counselors. However, recent studies showed that virtual counselors’ nonverbal compassion through eye contact, facial mimicry, and head-nodding also have significant impact on the overall counseling experience. To verify this, we designed the virtual counselor’s nonverbal compassion and examined its effects on counseling effectiveness (i.e., reduce the intensity of anger and improve general affect). A total of 40 participants were recruited from the university community. Participants were then randomly assigned to one of two virtual counselor conditions: a neutral virtual counselor condition without nonverbal compassion and a compassionate virtual counselor condition with nonverbal compassion (i.e., eye contact, facial mimicry, and head-nodding). Participants shared their anger-inducing episodes with the virtual counselor for an average of 16.30 min. Note that the virtual counselor was operated by the Wizard-of-Oz method without actually being technically implemented. Results showed that counseling with a compassionate virtual counselor reduced the intensity of anger significantly more than counseling with a neutral virtual counselor (F(1, 37) = 30.822, p < 0.001, ηp2 = 0.454). In addition, participants who counseled with a compassionate virtual counselor responded that they experienced higher empathy than those who counseled with a neutral virtual counselor (p < 0.001). These findings suggest that nonverbal compassion through eye contact, facial mimicry, and head-nodding of the virtual counselor makes the participants feel more empathy, which contributes to improving the counseling effectiveness by reducing the intensity of anger.
Intensionality is more cognitively demanding than false belief in adults: evidence from a non-inferential sentence-reading task
Authors: Yeung Elaine Kit Ling , Cheung Him 
Published: January 2024

AbstractChildren are said to understand false belief if they can appreciate an agent’s wrong description of an object as a result of misinformation, and intensionality if they can appreciate and switch between alternative descriptions from different epistemic viewpoints. Most previous studies have investigated the developmental trajectories of these capacities in the age range from 3 to 10 years aiming to discern their conceptual nature. The present research examines whether intensionality incurs lower performance accuracies and longer response times than false belief in adults, using a task in which participants read sentences that explicitly state an agent’s beliefs. Experiment 1 showed that participants were less accurate in rejecting verbal probes that contradicted an agent’s alternative than false thoughts about objects. Experiments 2 and 3 replicated this finding using thoughts about object identities but not properties. These results suggest that compared to false belief, intensionality is cognitively demanding for adults to process because of the availability of more than one identity candidate under the agent’s perspective.