bluuebunny/crossref_metadata_2025_split huggingface dataset:
The oncogenic mechanisms of the Janus kinase-signal transducer and activator of transcription pathway in digestive tract tumors
Authors: Zhao Ruihong , Hu Zhangmin , Zhang Xiaoli , Huang Shujuan , Yu Guodong , Wu Zhe , Yu Wei , Lu Juan , Ruan Bing 
Published: January 2024

AbstractDigestive tract tumors are heterogeneous and involve the dysregulation of multiple signaling pathways. The Janus kinase-signal transducer and activator of transcription (JAK–STAT) pathway plays a notable role in the oncogenesis of digestive tract tumors. Typically activated by pro-inflammatory cytokines, it regulates important biological processes, such as cell growth, differentiation, apoptosis, immune responses, and inflammation. The aberrant activation of this pathway manifests in different forms, including mutations in JAKs, overexpression of cytokine receptors, and sustained STAT activation, and contributes to promoting the malignant characteristics of cancer cells, including uncontrolled proliferation, resistance to apoptosis, enhanced invasion and metastasis, angiogenesis, acquisition of stem-like properties, and drug resistance. Numerous studies have shown that aberrant activation of the JAK-STAT pathway is closely related to the development and progression of digestive tract tumors, contributing to tumor survival, angiogenesis, changes in the tumor microenvironment, and even immune escape processes. In addition, this signaling pathway also affects the sensitivity of digestive tract tumors to chemotherapy and targeted therapy. Therefore, it is crucial to comprehensively understand the oncogenic mechanisms underlying the JAK-STAT pathway in order to develop effective therapeutic strategies against digestive tract tumors. Currently, several JAK–STAT inhibitors are undergoing clinical and preclinical trials as potential treatments for various human diseases. However, further investigation is required to determine the role of this pathway, as well as the effectiveness and safety of its inhibitors, especially in the context of digestive tract tumors. In this review, we provide an overview of the structure, classic activation, and negative regulation of the JAK-STAT pathway. Furthermore, we discuss the pathogenic mechanisms of JAK-STAT signaling in different digestive tract tumors, with the aim of identifying potential novel therapeutic targets.
Association of dietary inflammatory index with obesity among children and adolescents in the United States NHANES cohort: a population-based study
Authors: Zhang Lili , Peng Huimin , Wang Yao , Ba Hongjun 
Published: January 2024

Abstract
Background
Childhood obesity has become a huge challenge to childhood health, and there is a lack of understanding about the relationship between dietary inflammatory index (DII) and childhood obesity. The main objective of this study was to analyze the potential link between DII and obesity among children and adolescents residing in the United States.

Methods
A cross-sectional analysis was performed using data obtained from the National Health and Nutrition Examination Survey between 2009 and 2018. In total, 12,454 participants were included in the analysis. DII was calculated based on dietary data from the first day of the 24-hour dietary recall. Logistic regression was used to analyze the association between DII and obesity, as well as central obesity defined by the waist-to-height ratio of 0.5 or higher or waist circumference ≥ 90th percentile for age and sex.

Results
The mean dietary inflammation index was 2.05 (SE = 0.02), with higher levels in children than in adolescents (P = 0.01). According to our findings, the prevalence of central obesity was higher among adolescents (38.0%) than among children (31.4%). The adolescents in the third quartile of DII have a higher risk of overweight/obesity (OR = 1.46, 95% CI: 1.24–1.71) after adjusting for age, sex, and race. This positive association remained significant even after physical activity was added to the model. Concerning central obesity, the adolescents in the highest quartile of DII have a higher risk, independent of demographic characteristics and physical activity. However, no significant association was observed among children.

Conclusions
The dietary inflammation index was positively associated with overweight/obesity and central obesity among adolescents in the United States after adjusting for confounding factors. These findings highlight the importance of promoting anti-inflammatory diets in adolescents to prevent obesity and its associated complications.
Endoplasmic reticulum aminopeptidase 2 regulates CD4+ T cells pyroptosis in rheumatoid arthritis
Authors: Zhang Jianhua , Cai Hao , Sun Weiwei , Wu Weijie , Nan Yunyi , Ni Yingchen , Wu Xinyuan , Chen Minhao , Xu Hua , Wang Youhua 
Published: January 2024

Abstract
Objective
Rheumatoid arthritis (RA) is a chronic, progressive autoimmune disease with a complex pathogenesis that has not yet been fully elucidated, and T-cell pyroptosis is an important pathogenetic factor in RA. This study aimed to investigate the role of endoplasmic reticulum aminopeptidase 2 (ERAP2) in the pyroptosis of CD4+ T cells in RA and the specific molecular mechanism.

Methods
Peripheral venous blood was collected from human subjects, and CD4+ T cells were isolated and activated to measure the level of pyroptosis and ERAP2 expression. Pyroptosis levels were assessed using immunofluorescence, flow cytometry, qRT-PCR, and Western blotting. Changes in pyroptosis levels were observed upon knockdown or overexpression of ERAP2. To detect activated Caspase-1 in tissues, chimeric mice were engrafted with human synovial tissue and reconstituted with human CD4+ T cells. CD4 + T cells were treated with GLI1 antagonists and SMO receptor agonists to detect changes in pyroptosis levels.

Results
CD4+ T cell levels undergoing pyroptosis were found to be elevated in the blood and synovium of RA patients. The gene and protein expression of ERAP2 were significantly higher in CD4+ T cells from RA patients. Deletion of ERAP2 suppressed pyroptosis of these cells, attenuated the activation of Caspase-1 in tissue T cells, and reduced tissue inflammatory responses. Reciprocally, overexpression of ERAP2 triggered inflammasome assembly, activated Caspase-1, and induced pyroptosis in CD4+ T cells. Mechanistically, ERAP2 inhibits the Hedgehog signaling pathway and upregulates the expression of nucleotide-binding oligomerization segment-like receptor family 3(NLRP3), cleaved Caspase-1, and Gasdermin D to promote pyroptosis in CD4+ T cells.

Conclusions
Taken together, our results identify a novel mechanism by which ERAP2 regulates RA development and document the effect of the ERAP2/Hedgehog signaling axis on pyroptosis of CD4+ T cells from RA patients.
Endometriosis MR mimickers: T2-hypointense lesions
Authors: Ruaux Edouard , VanBuren Wendaline M. , Nougaret Stéphanie , Gavrel Marie , Charlot Mathilde , Grangeon Flavia , Bolze Pierre-Adrien , Thomassin-Naggara Isabelle , Rousset Pascal 
Published: January 2024

AbstractEndometriosis is a common crippling disease in women of reproductive age. Magnetic resonance imaging (MRI) is considered the cornerstone radiological technique for both the diagnosis and management of endometriosis. While its sensitivity, especially in deep infiltrating endometriosis, is superior to that of ultrasonography, many sources of false-positive results exist, leading to a lack of specificity. Hypointense lesions or pseudo-lesions on T2-weighted images include anatomical variants, fibrous connective tissues, benign and malignant tumors, feces, surgical materials, and post treatment scars which may mimic deep pelvic infiltrating endometriosis. False positives can have a major impact on patient management, from diagnosis to medical or surgical treatment. This educational review aims to help the radiologist acknowledge MRI criteria, pitfalls, and the differential diagnosis of deep pelvic infiltrating endometriosis to reduce false-positive results.Critical relevance statementMRI in deep infiltrating endometriosis has a 23% false-positive rate, leading to misdiagnosis. T2-hypointense lesions primarily result from anatomical variations, fibrous connective tissue, benign and malignant tumors, feces, surgical material, and post-treatment scars.Key points• MRI in DIE has a 23% false-positive rate, leading to potential misdiagnosis.• Anatomical variations, fibrous connective tissues, neoplasms, and surgical alterations are the main sources of T2-hypointense mimickers.• Multisequence interpretation, morphologic assessment, and precise anatomic localization are crucial to prevent overdiagnosis.• Gadolinium injection is beneficial for assessing endometriosis differential diagnosis only in specific conditions.
Graphical Abstract
Regenerative therapies for femoral head necrosis in the past two decades: a systematic review and network meta-analysis
Authors: Wang Xiaole , Hu Liyou , Wei Bo , Wang Jian , Hou Decai , Deng Xiaolei 
Published: January 2024

Abstract
Background
Regenerative techniques combined with core decompression (CD) are commonly used to treat osteonecrosis of the femoral head (ONFH). However, no consensus exists on regeneration therapy combined with CD that performs optimally. Therefore, we evaluated six regenerative therapies combined with CD treatment using a Bayesian network meta-analysis (NMA).

Methods
We searched PubMed, Embase, Cochrane Library, and Web of Science databases. Six common regeneration techniques were categorized into the following groups with CD as the control group: (1) autologous bone graft (ABG), (2) autologous bone graft combined with bone marrow aspirate concentrate (ABG + BMAC), (3) bone marrow aspirate concentrate (BMAC), (4) free vascular autologous bone graft (FVBG), (5) expanded mesenchymal stem cells (MSCs), and (6) platelet-rich plasma (PRP). The conversion rate to total hip arthroplasty (THA) and progression rate to femoral head necrosis were compared among the six treatments.

Result
A total of 17 literature were included in this study. In the NMA, two of the six treatment strategies demonstrated higher response in preventing the progression of ONFH than CD: MSCs (odds ratio [OR]: 0.098, 95% confidence interval [CI]: 0.0087–0.87) and BMAC (OR: 0.27, 95% CI: 0.073–0.73). Additionally, two of the six treatment strategies were effective techniques in preventing the conversion of ONFH to THA: MSCs (OR: 0.062, 95% CI: 0.0038–0.40) and BMAC (OR: 0.32, 95% CI: 0.1–0.074). No significant difference was found among FVBG, PRP, ABG + BMAC, ABG, and CD in preventing ONFH progression and conversion to THA (P > 0.05).

Conclusions
Our NMA found that MSCs and BMAC were effective in preventing ONFH progression and conversion to THA among the six regenerative therapies. According to the surface under the cumulative ranking value, MSCs ranked first, followed by BMAC. Additionally, based on our NMA results, MSCs and BMAC following CD may be necessary to prevent ONFH progression and conversion to THA. Therefore, these findings provide evidence for the use of regenerative therapy for ONFH.
A novel AML1-ETO/FTO positive feedback loop promotes leukemogenesis and Ara-C resistance via stabilizing IGFBP2 in t(8;21) acute myeloid leukemia
Authors: Zhou Wei , Li Siying , Wang Hong , Zhou Jingfeng , Li Shuyi , Chen Guofeng , Guan Wei , Fu Xianli , Nervi Clara , Yu Li , Li Yonghui 
Published: January 2024

Abstract
Background
t(8;21)(q22;q22) is one of the most frequent chromosomal abnormalities in acute myeloid leukemia (AML), leading to the generation of the fusion protein AML1-ETO. Despite t(8;21) AML being considered as a subtype with a favorable prognosis, approximately 30–50% of patients experience drug resistance and subsequent relapse. N6-methyladenosine (m6A) is demonstrated to be involved in the development of AML. However, the regulatory mechanisms between AML1-ETO and m6A-related enzymes and the roles of dysregulated m6A modifications in the t(8;21)-leukemogenesis and chemoresistance remain elusive.

Methods
Chromatin immunoprecipitation, dual-luciferase reporter assay, m6A-qPCR, RNA immunoprecipitation, and RNA stability assay were used to investigate a regulatory loop between AML1-ETO and FTO, an m6A demethylase. Gain- and loss-of-function experiments both in vitro and in vivo were further performed. Transcriptome-wide RNA sequencing and m6A sequencing were conducted to identify the potential targets of FTO.

Results
Here we show that FTO is highly expressed in t(8;21) AML, especially in patients with primary refractory disease. The expression of FTO is positively correlated with AML1-ETO, which is attributed to a positive regulatory loop between the AML1-ETO and FTO. Mechanistically, AML1-ETO upregulates FTO expression through inhibiting the transcriptional repression of FTO mediated by PU.1. Meanwhile, FTO promotes the expression of AML1-ETO by inhibiting YTHDF2-mediated AML1-ETO mRNA decay. Inactivation of FTO significantly suppresses cell proliferation, promotes cell differentiation and renders resistant t(8;21) AML cells sensitive to Ara-C. FTO exerts functions by regulating its mRNA targets, especially IGFBP2, in an m6A-dependent manner. Regain of Ara-C tolerance is observed when IGFBP2 is overexpressed in FTO-knockdown t(8;21) AML cells.

Conclusion
Our work reveals a therapeutic potential of targeting AML1-ETO/FTO/IGFBP2 minicircuitry in the treatment for t(8;21) patients with resistance to Ara-C.
The influence of different clay/sand ratios on the hygrothermal properties of earthen plasters in the Maijishan Grottoes
Authors: Yao Shanshan , Yan Zengfeng , Xu Bokai , Bi Wenbei , Zhang Junjie , Li Hao , Qu Junhui , Zhang Songhao 
Published: January 2024

Abstract
Variations in temperature and moisture content in the earthen plasters of the Maijishan Grottoes can readily lead to mural deterioration. This study investigates the influence of two different clay/sand ratios on the hygrothermal properties of earthen plasters in the Maijishan Grottoes. Two distinct types of earthen plasters samples were created with different clay/sand ratios: one with a weight ratio of 30:70 and another with a weight ratio of 70:30. The hygrothermal properties of earthen plasters samples were examined, and the temperature and moisture content variations were evaluated based on measurement data of hygrothermal environment in three caves. The findings revealed that the influence of the clay/sand ratios on thermal properties was relatively minor compared to its effect on hygric properties. Lower clay/sand ratio resulted in decreased thermal conductivity and specific heat capacity of earthen plasters, leading to increased temperature fluctuation, but the difference is minor. Additionally, in high-humidity environments, the difference in moisture content variations between the two types is greater than in low-humidity environments as the relative humidity changes. This research holds significant implications for the preservation of murals in the Maijishan Grottoes while also providing fresh perspectives for the restoration of multi-layered mural structures.
Dynamic immobilization of bacterial cells on biofilm in a polyester nonwoven chemostat
Authors: Zhang Chao-Lei , Wang Chao , Dong Yue-Sheng , Sun Ya-Qin , Xiu Zhi-Long 
Published: January 2024

AbstractCell immobilization plays an important role in biocatalysis for high-value products. It is necessary to maintain the viability of immobilized cells for bioconversion using viable cells as biocatalysts. In this study, a novel polyester nonwoven chemostat was designed for cell immobilization to investigate biofilm formation and the dynamic balance between adsorption and desorption of cells on polyester nonwoven. The polyester nonwoven was suitable for cell immobilization, and the cell numbers on the polyester nonwoven can reach 6.5 ± 0.38 log CFU/mL. After adding the polyester nonwoven to the chemostat, the fluctuation phenomenon of free bacterial cells occurred. The reason for this phenomenon was the balance between adsorption and desorption of bacterial cells on the polyester nonwoven. Bacterial cells could adhere to the surface of polyester nonwoven via secreting extracellular polymeric substances (EPS) to form biofilms. As the maturation of biofilms, some dead cells inside the biofilms can cause the detachment of biofilms. This process of continuous adsorption and desorption of cells can ensure that the polyester nonwoven chemostat has lasting biological activity.
Graphical Abstract
Children’s cloth face mask sizing and digital fit analysis: method development
Authors: Maher Mona , Du Puis Jenny Leigh , Goodge Katarina , Frey Margaret , Park Heeju Terry , Baytar Fatma 
Published: January 2024

AbstractThere is a necessity to use digital data and tools when developing children’s products. The present study was designed to provide digital methods to guide product development and problem-solving when using 3D body scans and face mask simulations for 6-year-olds. First, key facial measurements were evaluated to better understand the variables that might affect face mask sizing for children for the selected age group. Then the findings were used to optimize the size and fit of a cloth face mask design. Next, the fit of the digital, optimized face mask design was tested on 44 head scans from Size North America by using subjective and objective fit assessment techniques. Study findings suggested that width and length-related measurements are critical for children’s face masks. Body mass index (BMI) and ethnicity were also found to be the main factors for identifying size ranges in the selected age group. As BMIs increase, face mask sizes should increase. Additionally, the results indicated a need to use a larger database of children of all ethnicities to design an inclusive facemask that would provide a comfortable and protective fit for different facial proportions. Although the results cannot be generalized due to the case study approach of the present research and its focus on methods development, they can provide manufacturers, designers, and researchers with guidelines on how to develop proper sizing and use digital data to conduct functional fit analysis for facemasks.
Assessing portfolio vulnerability to systemic risk: a vine copula and APARCH-DCC approach
Authors: Mba Jules Clement 
Published: January 2024

AbstractThis study evaluates the sensitivity and robustness of the systemic risk measure, Conditional Value-at-Risk (CoVaR), estimated using the vine copula and APARCH-DCC models. We compute the CoVaR for the two portfolios across five allocation strategies. The novel vine copula captures the complex dependence patterns and tail dynamics. The APARCH DCC incorporates volatility clustering, skewness, and kurtosis. The results reveal that the CoVaR estimates vary based on portfolio strategy, with higher values for the cryptocurrency portfolio. However, CoVaR appears relatively robust across strategies compared to ΔCoVaR. The cryptocurrency portfolio has a greater overall vulnerability. The findings demonstrate the value of CoVaR estimated via the vine copula and APARCH-DCC in assessing portfolio systemic risk. This advanced approach provides nuanced insights into strengthening risk management practices. Future research could explore the sensitivity of the CoVaR to different weighting schemes, such as equal versus market-weighted portfolios. Incorporating the Gram–Charlier expansion of normal density into the APARCH specification enables a nonparametric, data-driven fitting of the residual distribution. Furthermore, comparing the CoVaR to another systemic risk measure could provide further insights into its reliability as a systemic risk measure.
“They should’ve talked to us more”: lay health advisors’ experiences with community-engaged hypertension research
Authors: Collins Cyleste C. , Shediac-Rizkallah Mona , Dolata Jacqueline , Hood Erika , Nonguierma Elodie , Thornton Daryl 
Published: January 2024

Abstract
Background
Lay health advisors (LHAs) are increasingly being used to increase patient and public involvement in research, disseminate health information, and work toward preventing health disparities within communities at risk. This research explored LHAs’ experiences with training and recruiting for a hypertension research project which ended due to minimal enrollment.

Methods
The methodological design was qualitative description. One face-to-face semi-structured focus group was held with eight African American LHAs in Cleveland, Ohio, in the fall of 2019. The focus group was digitally recorded and transcribed by a professional transcriptionist and thematically analyzed.

Results
Trainees reflected on how much they learned from the training and described feeling passionate and excited about their community work for the project. We identified three key themes from the data: (1) Systemic and Institutional Factors Affected LHAs’ Experiences (subthemes: Unnecessarily Burdensome Requirements and Exploitation of Community Members for Research Gain; (2) Feeling Used Yet Unseen: Exclusion from Decision-Making Processes; (3) Worrying that Project Termination Damaged their Reputation; and (4) Disengaging from Research. We share lessons learned, including the need for LHAs’ expertise to be integrated into research studies, and for projects to establish clear communication and expectations regarding research rigor and requirements.

Conclusion
Our results have implications for future studies attempting to build equitable and strong academic-community relationships to yield rigorous and useful research to reduce health disparities.
Prognostic impact of KIHON checklist score in elderly patients with hemodialysis initiation
Authors: Fujioka Hayato , Koike Tsutomu , Imamura Teruhiko , Yokoyama Shingo , Murai Sayaka , Kakeshita Kota , Yamazaki Hidenori , Kinugawa Koichiro 
Published: January 2024

Abstract
Background
Frailty pervades the demographic of individuals afflicted by chronic kidney disease (CKD) and exhibits a robust correlation with a less favorable prognosis. Nonetheless, the evaluation and prognostication of frailty within the incipient stages of dialysis initiation remain shrouded in ambiguity. The necessity arises for an uncomplicated metric that holistically assesses frailty among aged CKD patients, one which lends itself to pragmatic clinical application.

Methods
In our prospective endeavor, we conducted assessments using the “KIHON checklist (KCL),” a questionnaire comprising 25 points, thoughtfully crafted by the Japanese Ministry of Health, Labour and Welfare to provide a thorough evaluation of the elderly population. This assessment was administered at the commencement of hemodialysis in patients aged 65 years or older. Subsequently, we analyzed the prognostic ramifications of the initial KCL scores on the composite primary endpoint, which encompassed the undesirable outcomes of either being bedridden or succumbing to mortality.

Results
A total of 24 patients (median age 76 years, 20 men) were included. The median KCL score was 6 [4, 10] points, and it was significantly correlated with the number of components in the revised Japanese Cardiovascular Health Study, which is a diagnostic criterion for frailty (p < 0.05). The group with a KCL score ≥ 10 points had a significantly lower 1-year freedom from the primary endpoints than the other group (43% versus 87%, p < 0.05). Among the components of the KCL, physical strength was significantly associated with the prognosis (p < 0.05).

Conclusion
The screening protocol employing the KCL during the commencement of hemodialysis among elderly individuals proved to be a valuable tool for the anticipation of both the state of being bedridden and mortality.