bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction and validation of a novel prognostic model of neutrophil‑related genes signature of lung adenocarcinoma
Authors: Zhu Qianjun , Chai Yanfei , Jin Longyu , Ma Yuchao , Lu Hongwei , Chen Yingji , Feng Wei 
Published: October 2023

AbstractLung adenocarcinoma (LUAD) remains an incurable disease with a poor prognosis. This study aimed to explore neutrophil‑related genes (NRGs) and develop a prognostic signature for predicting the prognosis of LUAD. NRGs were obtained by intersecting modular genes identified by weighted gene co-expression network analysis (WGCNA) using bulk RNA-seq data and the marker genes of neutrophils identified from single-cell RNA-sequencing(scRNA-seq) data. Univariate Cox regression, least absolute shrinkage and selection operator (LASSO), and multivariate Cox analyses were run to construct a prognostic signature, follow by delineation of risk groups, and external validation. Analyses of ESTIMAT, immune function, Tumor Immune Dysfunction and Exclusion (TIDE) scores, Immune cell Proportion Score (IPS), and immune checkpoint genes between high- and low-risk groups were performed, and then analyses of drug sensitivity to screen for sensitive anticancer drugs in high-risk groups. A total of 45 candidate NRGs were identified, of which PLTP, EREG, CD68, CD69, PLAUR, and CYP27A1 were considered to be significantly associated with prognosis in LUAD and were used to construct a prognostic signature. Correlation analysis showed significant differences in the immune landscape between high- and low-risk groups. In addition, our prognostic signature was important for predicting drug sensitivity in the high-risk group. Our study screened for NRGs in LUAD and constructed a novel and effective signature, revealing the immune landscape and providing more appropriate guidance protocols in LUAD treatment.
Patient-centric assessment of rheumatoid arthritis using a smartwatch and bespoke mobile app in a clinical setting
Authors: Hamy Valentin , Llop Christopher , Yee Christopher W. , Garcia-Gancedo Luis , Maxwell Aoife , Chen Wen Hung , Tomlinson Ryan , Bobbili Priyanka , Bendelac Julien , Landry Jessica , DerSarkissian Maral , Yenikomshian Mihran , Mody Elinor A. , Duh Mei Sheng , Williams Rachel 
Published: October 2023

AbstractRheumatoid arthritis (RA) is a fluctuating progressive disease requiring frequent symptom assessment for appropriate management. Continuous tracking using digital technologies may provide greater insights of a patient’s experience. This prospective study assessed the feasibility, reliability, and clinical utility of using novel digital technologies to remotely monitor participants with RA. Participants with moderate to severe RA and non-RA controls were monitored continuously for 14 days using an iPhone with an integrated bespoke application and an Apple Watch. Participants completed patient-reported outcome measures and objective guided tests designed to assess disease-related impact on physical function. The study was completed by 28 participants with RA, 28 matched controls, and 2 unmatched controls. Completion rates for all assessments were > 97% and were reproducible over time. Several guided tests distinguished between RA and control cohorts (e.g., mean lie-to-stand time [seconds]: RA: 4.77, control: 3.25; P < 0.001). Participants with RA reporting greater stiffness, pain, and fatigue had worse guided test performances (e.g., wrist movement [P < 0.001] and sit-to-stand transition time [P = 0.009]) compared with those reporting lower stiffness, pain, and fatigue. This study demonstrates that digital technologies can be used in a well-controlled, remote clinical setting to assess the daily impact of RA.
Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device
Authors: Sharma Manju , Sheth Maulee , Poling Holly M. , Kuhnell Damaris , Langevin Scott M. , Esfandiari Leyla 
Published: October 2023

AbstractNano-scale extracellular vesicles are lipid-bilayer delimited particles that are naturally secreted by all cells and have emerged as valuable biomarkers for a wide range of diseases. Efficient isolation of small extracellular vesicles while maintaining yield and purity is crucial to harvest their potential in diagnostic, prognostic, and therapeutic applications. Most conventional methods of isolation suffer from significant shortcomings, including low purity or yield, long duration, need for large sample volumes, specialized equipment, trained personnel, and high costs. To address some of these challenges, our group has reported a novel insulator-based dielectrophoretic device for rapid isolation of small extracellular vesicles from biofluids and cell culture media based on their size and dielectric properties. In this study, we report a comprehensive characterization of small extracellular vesicles isolated from cancer-patients’ biofluids at a twofold enrichment using the device. The three-fold characterization that was performed using conventional flow cytometry, advanced imaging flow cytometry, and microRNA sequencing indicated high yield and purity of the isolated small extracellular vesicles. The device thus offers an efficient platform for rapid isolation while maintaining biomolecular integrity.
Divergent strategies in cranial biomechanics and feeding ecology of the ankylosaurian dinosaurs
Authors: Ballell Antonio , Mai Bohao , Benton Michael J. 
Published: October 2023

Abstract
Ankylosaurs were important megaherbivores of Jurassic and Cretaceous ecosystems. Their distinctive craniodental anatomy and mechanics differentiated them from coexisting hadrosaurs and ceratopsians, and morphological evidence suggests dietary niche partitioning between sympatric ankylosaurids and nodosaurids. Here, we investigate the skull biomechanics of ankylosaurs relative to feeding function. First, we compare feeding functional performance between nodosaurids and ankylosaurids applying finite element analysis and lever mechanics to the skulls of Panoplosaurus mirus (Nodosauridae) and Euoplocephalus tutus (Ankylosauridae). We also compare jaw performance across a wider sample of ankylosaurs through lever mechanics and phylogenetic comparative methods. Mandibular stress levels are higher in Euoplocephalus, supporting the view that Panoplosaurus consumed tougher foodstuffs. Bite force and mechanical advantage (MA) estimates indicate that Panoplosaurus had a relatively more forceful and efficient bite than Euoplocephalus. There is little support for a role of the secondary palate in resisting feeding loads in the two ankylosaur clades. Several ankylosaurs converged on similar jaw mechanics, while some nodosaurids specialised towards high MA and some ankylosaurids evolved low MA jaws. Our study supports the hypothesis that ankylosaurs partitioned dietary niches in Late Cretaceous ecosystems and reveals that the two main ankylosaur clades evolved divergent evolutionary pathways in skull biomechanics and feeding habits.
Integrated biophysical matching of bacterial nanocellulose coronary artery bypass grafts towards bioinspired artery typical functions
Authors: Hülsmann Jörn , Fraune Theresa , Dodawatta Baratha , Reuter Fabian , Beutner Martin , Beck Viktoria , Hackert-Oschätzchen Matthias , Ohl Claus Dieter , Bettenbrock Katja , Janiga Gabor , Wippermann Jens , Wacker Max 
Published: October 2023

AbstractRevascularization via coronary artery bypass grafting (CABG) to treat cardiovascular disease is established as one of the most important lifesaving surgical techniques worldwide. But the shortage in functionally self-adaptive autologous arteries leads to circumstances where the clinical reality must deal with fighting pathologies coming from the mismatching biophysical functionality of more available venous grafts. Synthetic biomaterial-based CABG grafts did not make it to the market yet, what is mostly due to technical hurdles in matching biophysical properties to the complex demands of the CABG niche. But bacterial Nanocellulose (BNC) Hydrogels derived by growing biofilms hold a naturally integrative character in function-giving properties by its freedom in designing form and intrinsic fiber architecture. In this study we use this integral to combine impacts on the luminal fiber matrix, biomechanical properties and the reciprocal stimulation of microtopography and induced flow patterns, to investigate biomimetic and artificial designs on their bio-functional effects. Therefore, we produced tubular BNC-hydrogels at distinctive designs, characterized the structural and biomechanical properties and subjected them to in vitro endothelial colonization in bioreactor assisted perfusion cultivation. Results showed clearly improved functional properties and gave an indication of successfully realized stimulation by artery-typical helical flow patterns.
Automated maternal behavior during early life in rodents (AMBER) pipeline
Authors: Lapp Hannah E. , Salazar Melissa G. , Champagne Frances A. 
Published: October 2023

AbstractMother-infant interactions during the early postnatal period are critical for infant survival and the scaffolding of infant development. Rodent models are used extensively to understand how these early social experiences influence neurobiology across the lifespan. However, methods for measuring postnatal dam-pup interactions typically involve time-consuming manual scoring, vary widely between research groups, and produce low density data that limits downstream analytical applications. To address these methodological issues, we developed the Automated Maternal Behavior during Early life in Rodents (AMBER) pipeline for quantifying home-cage maternal and mother–pup interactions using open-source machine learning tools. DeepLabCut was used to track key points on rat dams (32 points) and individual pups (9 points per pup) in postnatal day 1–10 video recordings. Pose estimation models reached key point test errors of approximately 4.1–10 mm (14.39 pixels) and 3.44–7.87 mm (11.81 pixels) depending on depth of animal in the frame averaged across all key points for dam and pups respectively. Pose estimation data and human-annotated behavior labels from 38 videos were used with Simple Behavioral Analysis (SimBA) to generate behavior classifiers for dam active nursing, passive nursing, nest attendance, licking and grooming, self-directed grooming, eating, and drinking using random forest algorithms. All classifiers had excellent performance on test frames, with F1 scores above 0.886. Performance on hold-out videos remained high for nest attendance (F1 = 0.990), active nursing (F1 = 0.828), and licking and grooming (F1 = 0.766) but was lower for eating, drinking, and self-directed grooming (F1 = 0.534–0.554). A set of 242 videos was used with AMBER and produced behavior measures in the expected range from postnatal 1–10 home-cage videos. This pipeline is a major advancement in assessing home-cage dam-pup interactions in a way that reduces experimenter burden while increasing reproducibility, reliability, and detail of data for use in developmental studies without the need for special housing systems or proprietary software.
Effect of surface topography and wettability on shear bond strength of Y-TZP ceramic
Authors: Wongsue Suriyakul , Thanatvarakorn Ornnicha , Prasansuttiporn Taweesak , Nimmanpipug Piyarat , Sastraruji Thanapat , Hosaka Keiichi , Foxton Richard M. , Nakajima Masatoshi 
Published: October 2023

AbstractZirconia ceramics have been widely used as dental restorations due to their esthetic appearance and high flexural strength. The bonding of zirconia with resin cement should rely on both mechanical and chemical bonds. This study was performed to investigate the effect of zirconia surface topography and its wettability after surface pretreatments on the microshear bond strength (μSBS) of a resin cement. Zirconia slabs were prepared and randomly divided into 5 groups based on the surface treatment as follows: no treatment (control), air abrasion (AB), etching with hydrofluoric acid (F), the mixture of hydrofluoric acid and nitric acid (FN), or the mixture of hydrochloric acid and nitric acid (CN) for 10 min. The specimens were subjected to investigation of surface roughness characteristics [average roughness (Ra), peak-to-valley average distance (Rpv), skewness (Rsk), and kurtosis (Rku)] using atomic force microscopy (AFM) and measurements of surface contact angle (θc) and μSBS of a resin cement. In addition, the area % of the nanoscale surface irregularity (nSI%) was calculated from the AFM images. The effects of nSI%, Ra and θc on the μSBS were analyzed by multiple linear regression analysis (p < 0.05). Multiple regression analysis revealed that the nSI% was the most predominant factor for the μSBS (p < 0.001). A surface with larger nSI%, higher Ra and relatively lower θc was essential for establishing a reliable resin-zirconia bond.
Corrosion-driven droplet wetting on iron nanolayers
Authors: Ricard Aurelien , Restagno Frederic , Jang Yun Hee , Lansac Yves , Raspaud Eric 
Published: October 2023

AbstractThe classical Evans’ drop describes a drop of aqueous salt solution, placed on a bulk metal surface where it displays a corrosion pit that grows over time producing further oxide deposits from the metal dissolution. We focus here on the corrosion-induced droplet spreading using iron nanolayers whose semi-transparency allowed us to monitor both iron corrosion propagation and electrolyte droplet behavior by simple optical means. We thus observed that pits grow under the droplet and merge into a corrosion front. This front reached the triple contact line and drove a non radial spreading, until it propagated outside the immobile droplet. Such chemically-active wetting is only observed in the presence of a conductive substrate that provides strong adhesion of the iron nanofilm to the substrate. By revisiting the classic Evan’s drop experiment on thick iron film, a weaker corrosion-driven droplet spreading is also identified. These results require further investigations, but they clearly open up new perspectives on substrate wetting by corrosion-like electrochemical reactions at the nanometer scale.
Study on pore structure and the mechanical properties of sandstone-concrete binary under freeze–thaw environment
Authors: Hu Anlong , Xue Guobin , Shang Zhipeng , Cao Zhe , Wang Xiaoping , Fu Yintao , Huang Xiaoqing 
Published: October 2023

AbstractIn China's cold region water conservancy and hydropower projects, the contact interface between the dam and the reservoir bank rock is prone to cracking under external loading and freeze–thaw action, which may lead to dam-bank failure and damage and cause engineering disasters. The NMR (Nuclear Magnetic Resonance) tests and uniaxial compression tests of concrete, sandstone, and sandstone-concrete composite after different numbers of freeze–thaw cycles were carried out to analyze the pore structure development and uniaxial compression mechanical properties of the three types of specimens under different numbers of freeze–thaw cycles. The results show that freeze–thaw cycling promotes the development of pores in sandstone and concrete, and sandstone is more sensitive to low-temperature freeze–thaw than concrete. The UCS (uniaxial compressive strength) of the sandstone-concrete binary changed in a V-shaped with the increase of the dip angle of the cemented interface, and the angle had no obvious effect on the microscopic pores. The freeze–thaw effect on the deterioration of the microscopic pore structure and mechanical properties of the sandstone-concrete binary has a similar effect pattern, in which the deterioration rate of porosity and compressive strength is faster in the early freeze–thaw period, slower in the middle period, and increases in the later period compared with the middle period, but the increase is smaller than that in the early period of freeze–thaw. In addition, the relationship between the porosity and UCS of the sandstone-concrete binary under the freeze–thaw cycle environment is a quadratic parabola.
Association between iron status markers and kidney outcome in patients with chronic kidney disease
Authors: Fujisawa Hironobu , Nakayama Masaru , Haruyama Naoki , Fukui Akiko , Yoshitomi Ryota , Tsuruya Kazuhiko , Nakano Toshiaki , Kitazono Takanari 
Published: October 2023

AbstractSeveral studies conducted in patients with various stages of chronic kidney disease (CKD) have investigated the association of iron status markers, such as transferrin saturation (TSAT) and serum ferritin, with kidney outcomes. However, the associations were inconsistent and remain strongly debated. Therefore, we aimed to investigate whether TSAT and serum ferritin levels were associated with kidney outcome in such a population. In this study, 890 patients who were admitted for the evaluation of and education for CKD were prospectively followed. Primary kidney outcome was a composite of doubling of serum creatinine, end-stage kidney disease, or death due to kidney failure. Participants were divided into quartiles (Q1–Q4) according to TSAT or serum ferritin levels. During a median follow-up period of 2.8 years, kidney events occurred in 358 patients. In the multivariable Cox analyses, compared with Q3 of TSAT, the hazard ratios (95% confidence intervals) for Q1, Q2, and Q4 were 1.20 (0.87, 1.66), 1.38 (1.01, 1.87), and 1.14 (0.82, 1.59), respectively. Compared with Q2 of serum ferritin, lower and higher quartiles had a significantly increased risk for kidney outcome; hazard ratios (95% confidence intervals) for Q1, Q3, and Q4 were 1.64 (1.18, 2.27), 1.71 (1.24, 2.37), and 1.52 (1.10, 2.10), respectively. A Fine-Gray model with death before kidney events as a competing risk showed results similar to the above. In CKD, lower and higher ferritin levels were independent risk factors for kidney disease progression.
Clinical outcomes and prognostic factors in the surgical treatment of spinal dural arteriovenous fistulas: a retrospective study of 118 patients
Authors: Peng Youheng , Ren Yanming , Hou Jiguang , Zhang Changwei , He Min , Huang Bowen , Chen Tengyun , Li Jin 
Published: October 2023

AbstractSpinal dural arteriovenous fistulas (SDAVFs) are the most common type of spinal vascular malformations (AVMs), constituting approximately 70% of all spinal AVMs. The impact of various clinical and radiologic features on the outcomes in patients with SDAVFs is still controversial. The purpose of the study is to investigate the clinical outcomes and prognostic factors in patients with surgically treated SDAVFs in a single center. A retrospective review was performed for all patients with SDAVFs from January 2013 to September 2021 who underwent surgery at our institution. Medical records and pre- and postoperative imaging data were analyzed. Neurological function status was evaluated by modified Aminoff-Logue Scale (mALS). Student’s t-test, Wilcoxon rank sum test, χ2 test and logistic regression were used to find significant prognostic factors. P values < 0.05 were considered significant. One hundred and eighteen patients were ultimately included in the study. By comparing preoperative and postoperative mALS, 69 (58.5%) patients experienced improvement, and 49 (41.5%) patients showed no improvement (worse or unchanged). Wilcoxon rank sum test showed that there was a difference between the improvement group and the no improvement group in preoperative mALS Micturition score and preoperative mALS Defecation score. The logistic regression revealed that preoperative mALS Gait score was associated with clinical improvement after surgery in patients with SDAVFs. Surgical treatment of SDAVFs is a safe and effective procedure and can lead to symptom improvement or stabilization in most patients. Preoperative neurological function status was the only factor associated with clinical prognosis.
Exploring the determinants influencing suicidal ideation and depression in gastrointestinal cancer patients
Authors: Choudhury Avishek , Shahsavar Yeganeh 
Published: October 2023

AbstractStudies have shown a heightened prevalence of depression and suicidal ideation among patients with Gastrointestinal Cancer (GIC). GIC patients are at a 1.5- to threefold increased risk of suicide and depression compared to other cancer patients. This study investigates the interplay of internet use, family burden, and emotional support on mental health (depression) and suicidal ideation among patients with GIC. The study involves 202 respondents of which 78 were undergoing GIC treatment during this study. Using structural equation modeling, our findings indicate a substantial negative correlation between mental health and suicidal ideation. Overall, suicidal ideation (median score) was noticeably lower in patient who completed their treatment with noticeable individuals with exceptionally high SI even after completing the treatment. Notably, participants who had completed their treatment demonstrated a significantly stronger correlation between emotional support and mental health compared to those who were still undergoing treatment. Age was found to moderate the mental health-suicidal ideation link significantly. Internet usage for health-related information was also inversely correlated with mental health (directly) and suicidal ideation (indirectly). We noted that the influence of emotional support on mental health was significantly higher among individuals who completed their treatment compared to those who were undergoing their GIC treatment. Family burden emerged as significant negative influences on mental health, while emotional support positively impacted mental health. The findings of this study contribute towards a deeper understanding of suicide risk factors in GIC patients, potentially shaping more effective preventive strategies.
FGFR blockade inhibits targeted therapy-tolerant persister in basal FGFR1- and FGF2-high cancers with driver oncogenes
Authors: Furugaki Koh , Fujimura Takaaki , Mizuta Hayato , Yoshimoto Takuya , Asakawa Takashi , Yoshimura Yasushi , Yoshiura Shigeki 
Published: October 2023

AbstractCancer cell resistance arises when tyrosine kinase inhibitor (TKI)-targeted therapies induce a drug-tolerant persister (DTP) state with growth via genetic aberrations, making DTP cells potential therapeutic targets. We screened an anti-cancer compound library and identified fibroblast growth factor receptor 1 (FGFR1) promoting alectinib-induced anaplastic lymphoma kinase (ALK) fusion-positive DTP cell’s survival. FGFR1 signaling promoted DTP cell survival generated from basal FGFR1- and fibroblast growth factor 2 (FGF2)-high protein expressing cells, following alectinib treatment, which is blocked by FGFR inhibition. The hazard ratio for progression-free survival of ALK-TKIs increased in patients with ALK fusion-positive non-small cell lung cancer with FGFR1- and FGF2-high mRNA expression at baseline. The combination of FGFR and targeted TKIs enhanced cell growth inhibition and apoptosis induction in basal FGFR1- and FGF2-high protein expressing cells with ALK-rearranged and epidermal growth factor receptor (EGFR)-mutated NSCLC, human epidermal growth factor receptor 2 (HER2)-amplified breast cancer, or v-raf murine sarcoma viral oncogene homolog B1 (BRAF)-mutated melanoma by preventing compensatory extracellular signal-regulated kinase (ERK) reactivation. These results suggest that a targeted TKI-induced DTP state results from an oncogenic switch from activated oncogenic driver signaling to the FGFR1 pathway in basal FGFR1- and FGF2-high expressing cancers and initial dual blockade of FGFR and driver oncogenes based on FGFR1 and FGF2 expression levels at baseline is a potent treatment strategy to prevent acquired drug resistance to targeted TKIs through DTP cells regardless of types of driver oncogenes.