bluuebunny/crossref_metadata_2025_split huggingface dataset:
Care Seeking Behavior and Treatment Gap for Mental Health Conditions in Bangladesh: Evidence from the National Mental Health Survey 2019
Authors: Huque Rumana , Azad Abul Kalam , Islam Khaleda , Ahmed Helal Uddin , Amin Mohammad Robed 
Published: December 2023

Abstract
Background: The study provides evidence of the existing pattern of mental healthcare-seeking behavior and treatment gaps among the adult population in Bangladesh, and identifies the factors that influence the decision of the patients with mental illness to seek mental health care.
Methods:We used the National Mental Health Survey Bangladesh 2019 dataset with 7270 households to identify the patterns of and facilitators for seeking mental health care in Bangladesh. A Probit model using a standard normal cumulative distribution function (CDF) with three specifications has been applied to identify the factors influencing mental healthcare-seeking behavior and the probability of seeking mental healthcare services in Bangladesh.
Results:About 19% of the total adult population have mental health disorder in the country. Among the patinets with mental illness, only 10% seek healthcare services from different sources of mental healthcare services. Patients with addictive disorder show the lowest interest in seeking healthcare services, while more patients receive treatment for bipolar disorder. The findings suggest that while 34.4% of bipolar disorder patients receive treatment for mental health, only 4.8% of patients with addictive disorder seek healthcare services. The Probit model shows that having other mentally disordered family members is the only statistically significant determinant among the socio-economic factors, such as gender, age, religion, education level and residential status, that influnec treatment seeking behaviour. The marginal effects analysis shows that the existence of mentally disordered family members increases the probability of seeking mental health care services by around 6%. The other socio-economic variables considered in the study are found statistically insignificant. However, a married woman has a significantly higher likelihood of seeking treatment than an unmarried woman, while the family size is the only variable that significantly influences treatment-seeking behavior for men.
Conclusion: Though mental health conditions are major public health concerns in Bangladesh, the treatment-seeking behavior among the people with mental diorder is very low, implying a large treatment gap for mental health conditions. The findings indicate the urgent need to increase mental health service coverage among mental health patients.
Transcriptome Profiling Across Multiple Organs Unveils Potential Roles of C-Fibers in Host Homeostatic Regulation in Mice
Authors: He Yu , Li Changgen , Wei Jianhua , Yang Zhongying , Ren Luo , Deng Yu , Meng Zijun , Zang Na , Liu Enmei 
Published: December 2023

Abstract
As nociceptors, C-fibers play a critical role in maintaining host homeostasis under both physiological and pathological conditions. We previously demonstrated that C-fiber degeneration confers protection against respiratory syncytial virus (RSV) infection. However, a comprehensive investigation on the effects of C-fiber degeneration on the physiological state of the host remains unexplored. To address this gap, we established a C-fiber-degenerated (KCF) BALB/c mouse model and validated it by immunofluorescence staining of multiple organs. We monitored the body weight of KCF mice and performed 16S rRNA sequencing of their feces. And their brains, lungs, intestines, and spleen were subjected to section staining and RNA sequencing. Although no significant changes in body weight or tissue pathology were observed, KCF mice showed significant tran-scriptional alterations in four examined organs. The lungs and intestines exhibited diminished proportions of resting mast cells, while the spleens displayed reduced proportions of monocytes. Functional enrichment analysis revealed the involvement of C-fibers in the production of immunoglobulin and changes in intestinal microbiota. Subsequent experiments confirmed a trend towards reduced globulin levels in the peripheral blood and marked alterations in the diversity and composition of intestinal microbiota. Integrated analysis of differentially expressed genes shared by all four organs identified the nucleotide-binding and oligomerization domain (NOD)-like receptor signaling pathway as a pivotal route by which C-fibers may influence these organs. In summary, this study elucidates the diverse regulatory roles that C-fibers play in maintaining host homeostasis, extending our understanding in a multi-organ context.
Orlicz spaces and their hyperbolic composition operators
Authors: Estaremi Yousef 
Published: December 2023

Abstract
In this paper, we provide equivalent conditions for composition operators to have the shadowing property on the Orlicz space $L^{\Phi}(\mu)$. Additionally, we show that for composition operators on Orlicz spaces, the concepts of generalized hyperbolicity and the shadowing property are equivalent. These results extend similar findings on $L^p$-spaces to Orlicz spaces.
Mitochondrial DNA D-Loop SNPs Unveil Molecular Signatures of Milk Production Variation in Murrah Buffalo
Authors: A.N Ampadi , E.M Sadeesh , - Madhuri S. Lahamge 
Published: December 2023

Abstract
Background
The Murrah buffalo, pivotal in Asian agriculture, faces challenges in maximizing milk production despite significant breeding efforts. Recognizing its economic importance, this investigates mtDNA D-loop variations in Murrah buffalo as potential indicators of milk production variability, addressing challenges in maximizing yield despite significant breeding efforts.
Methods & Results
Analyzing mtDNA D-loop sequences from 50 buffaloes, we categorized them into Low (Group 1), Medium (Group 2), and High ECM (Group 3) groups based on milk yields, fat and protein percentage of a 30-day period data. Somatic cell mtDNA D-loop analysis revealed distinct genetic variations, with significant differences among ECM groups. Group 2 showed higher SNP prevalence, group 3 had more insertions/deletions, and Group 1 exhibited the highest transition frequency. Notably, a consistent "C" deletion at the 714th position occurred in Groups 1 and 3, prevalent in 68% of Group 2. A G-A variation at the 93rd position was specific to the medium ECM group. Negative Tajima D values indicated unique variations in each group, with Group 1 having the highest number, and a specific SNP linked to Group 2 was identified. These SNPs in the D-loop region could impact mtDNA replication, influencing mitochondrial content among animals. Our results provides valuable insights into the role of mtDNA D-loop polymorphisms in milk production traits in Murrah buffalo.
Conclusions
Our research highlights the potential for valuable markers of cellular energy efficiency in Murrah buffalo. Exploring diverse cytoplasmic backgrounds opens avenues for mtDNA-based selection strategies, enhancing milk production and optimizing genetic traits for the dairy industry.
Heterologous expression of P9 from Akkermansia muciniphila improves the GLP-1 secretion of intestinal L cells
Authors: Di Wenxuan , Zhang Yuchen , Zhang Xinyuan , Han Luxuan , Zhao Liang , Hao Yanling , Zhai Zhengyuan 
Published: December 2023

Abstract

Glucagon-like peptide-1(GLP-1) is an incretin hormone secreted primarily from the intestinal L-cells in response to meals. GLP-1 is a key regulator of energy metabolism and food intake. It has been proven that P9 protein from A. muciniphila could increase GLP-1 release and improve glucose homeostasis in HFD-induced mice. To obtain an engineered Lactococcus lactiswhich produced P9 protein，mature polypeptide chain of P9 was codon-optimized, fused with N-terminal signal peptide Usp45, and expressed in L. lactis NZ9000. Heterologous secretion of P9 by recombinant L. lactis NZP9 were successfully detected by SDS-PAGE and western blotting. Notably, the supernatant of L. lactis NZP9 stimulated GLP-1 production of NCI-H716 cells. The relative expression level of GLP-1 biosynthesis gene GCG and PCSK1 were upregulated by 1.63 and 1.53 folds, respectively. The engineered strainL. lactis NZP9 was more effective than A. muciniphila BAA-835 in stimulating the intestinal L cells to produce GLP-1. To our knowledge, this is the first report on the secretory expression of carboxyl-terminal processing protease P9 from A. muciniphila in L. lactis.Our results suggest that genetically engineered L. lactis which expressed P9 may have therapeutic potential for the treatment of diabetes, obesity and other metabolic disorders.
Clinical features of bonding disorders in Japanese mothers derived from the Stafford Interview
Authors: Nishikii Yumi , Suetsugu Yoshiko , Yamashita Hiroshi , Yoshida Keiko 
Published: December 2023

Abstract
Background
This study aimed to systematically describe the characteristics of bonding disorders and diagnostically classify them using the Japanese version of the 6th Stafford Interview. We investigated the cut-off points of the Mother-Infant Bonding Scale (MIBS-J) and Postpartum Bonding Questionnaire (PBQ) to screen for bonding disorders.
Methods
We recruited participants in their second trimester and studied 40 mother-infant dyads. At one month postpartum, we conducted the mother-infant relationship section of the Stafford Interview and classified participants into diagnostic groups. We applied the MIBS-J at four days and one month postpartum and the PBQ at one month, combined with the interview. We used the total scores to analyse the receiver operating characteristic curve at four days and one month.
Results
We diagnosed one case of emotional rejection and eleven cases of mild disorder. Additionally, three cases exhibited pathological anger with mild disorder—one with emotional rejection and one infant-focused anxiety case with normal bonding. The screening scores for bonding disorders, including mild cases, were 2 points or more for MIBS-J at four days and 3 points or more at one month. The PBQ was better at identifying severe bonding disorders, scoring 19 points or more.
Conclusions
Bonding disorders can expose mothers to serious mental and parenting conditions as early as one month postpartum. Questionnaire screening and diagnostic interviews can help with early detection and care.
Flood-induced sediment deposition drives rapid invertebrate colonization of hyporheic zone
Authors: Negishi Junjiro N. 
Published: December 2023

Abstract

How hyporheic invertebrates at relatively shallow depths cope with floods and sediment movements that involve vertical and horizontal distribution shifts is not well known. This study examined the responses of macroinvertebrate communities in both benthic and hyporheic zone (30–50 cm deep from the riverbed) to a flow release at a fine temporal resolution (a few hours prior to and four days after an artificially caused flood) of a gravel-covered point bar so that immediate responses to the event can be described. Hyporheic communities exhibited little change during the flood, whereas benthic communities exhibited substantial decreases in total abundance. Most strikingly, at the taxon level, numerically dominant hyporheic species at relatively shallow depths, Alloperla ishikariana, increased substantially in the hyporheic zone, and its increase was positively correlated with deposited sediment depth (up to 45 cm). This study suggests that flood-induced sediment deposition drives rapid invertebrate colonization of the hyporheic zone. This can be perceived as refuge use of the benthic zone by hyporheic species in association with sediment deposits, which is a new conceptual framework for explaining how hyporheic invertebrates can persist through flood events by adapting to dynamic sediment movements. Experiments using artificial floods provide promising opportunities for challenging hyporheic studies.
Development of scaffold-free tissue-engineered constructs derived from mesenchymal stem cells with serum-free media for cartilage repair and long-term preservation
Authors: Maeda Satoshi , Matsumoto Masaya , Segawa Kotaro , Iwamoto Kaori , nakamura norimasa 
Published: December 2023

Abstract

Synovial mesenchymal stem cells (sMSCs) have great potential for cartilage repair, but their therapeutic design to avoid adverse effects associated with unknown factors remains a challenge. In addition, because long-term preservation is indispensable to maintain high quality levels until implantation, it is necessary to reduce their fluctuations. This study aimed to investigate the properties and feasibility of novel scaffold-free tissue-engineered constructs using serum-free media and develop long-term preservation methods. sMSCs were cultured in serum-free media, seeded at high density in a monolayer, and finally developed as a sheet-like construct called “gMSC1.” The properties of frozen gMSC1 (Fro-gMSC1) were compared with those of refrigerated gMSC1 (Ref-gMSC1) and then examined by their profile. Chondrogenic differentiation potential was analyzed by quantitative real-time polymerase chain reaction and quantification of glycosaminoglycan content. Xenografts into the cartilage defect model in rats were evaluated by histological staining. gMSC1 showed nearly similar properties independent of the preservation conditions. The animal experiment demonstrated that the defect could be filled with cartilage-like tissue with good integration to the adjacent tissue, suggesting that gMSC1 was formed and replaced the cartilage. Furthermore, several chondrogenesis-related factors were significantly secreted inside and outside gMSC1. Morphological analysis of Fro-gMSC1 revealed comparable quality levels to those of fresh gMSC1. Thus, if cryopreserved, gMSC1, with no complicated materials or processes, could have sustained cartilage repair capacity. gMSC1 is a prominent candidate in novel clinical practice for cartilage repair, allowing for large quantities to be manufactured at one time and preserved for a long term by freezing.
Global research landscape and trends of cancer stem cells: visualization biology analysis
Authors: Chen Yuxian , Qu Baozhen , Zheng Keke , Liu Yanhao , Zhang Xiaotao , Lu Linlin 
Published: December 2023

Abstract
Background
Cancer stem cells (CSCs) are a subset of cells with self-renewal ability and tumor generating potential. Accumulated evidence has revealed that CSCs were shown to contribute to tumorigenesis, metastasis, recurrence and resistance to chemoradiotherapy. Therefore, CSCs were regarded as promising therapeutic targets in cancer. This study aims to reveal the development process, research hotspots, and trends of CSCs research through bibliometric methods.
Methods
All relevant publications on CSCs with more than 100 citations during 1997–2023 were extracted from the Science Citation Index Expanded of Web of Science Core Collection (WoSCC), and related information was collected and analyzed using Microsoft Excel 2019, GraphPad Prism 8, VOSviewer, Citespace and “bibliometrix” packages of the R software.
Results
A total of 2521 papers were included. Cancer Research published the largest number of papers (184 papers). The USA accounted for the most publications (1326 papers). Rich, JN was the author with the most publications (56 papers) and the highest M-index (3.111). The most contributive institution was the University of Texas System (164 papers). Before 2007, research mainly focused on the definition and recognition of CSCs. Between 2007 and 2016, with the emergence of the terms such as "sonic hedgehog", "metabolism", “oxidative phosphorylation” and “epithelial mesenchymal transition”, research began to shift toward exploring the mechanisms of CSCs. In 2016, the focus transitioned to the tumor microenvironment and the ecological niches. The analysis of papers published in major journals since 2021 showed that “transcription”, “inhibition”, and “chemoresistance” emerged as new focused issues.
Conclusion
There has been a growing number of research on CSCs. The USA has always been a leading position in this field. In general, the research focus has gradually shifted from basic biology to clinical transformation. “Tumor microenvironment” and “chemoresistance” should be given more attention in the future.
Effects of fabric structure on the ballistic impact response of 3D angle- interlock woven fabric targets
Authors: Wei Qingsong , Chen Jiaxue , Yang Dan , Pan Zhongxiang 
Published: December 2023

Abstract
High-performance 3D fabrics are widely used for ballistic protection. In this study, we conducted finite element analysis (FEA) to investigate the ballistic mechanisms of 3D layer-to-layer angle-interlock woven fabric (3DLAWF) and 3D through-the-thickness angle-interlock woven fabrics (3DTAWF) under impact. We constructed full-scale mesoscale fabric models for two 3D fabrics to examine the influence of 3D fabric structures on dynamic behavior and ballistic performance. Additionally, by comparing and analyzing the energy absorption patterns and failure mechanisms of the fabrics under ballistic penetration, optimization strategies for 3D fabric structures were proposed. This work reveals the impact damage evolution mechanisms, energy absorption mechanisms, and stress wave distribution patterns in these two fabrics, providing valuable guidance for the design of ballistic protection using 3D fabrics.
Ten-Year Outcomes after Purified CD34+ Cells Transplantation in Patients with Angiitis-induced Critical Limb Ischemia
Authors: Liu Hao , Jiang Xiaolang , Zou Lingwei , Fang Yuan , Fang Gang , Liu Yifan , Chen Bin , Gu Shiyang , Wei Zheng , Liu Peng , Fu Weiguo , Pan Tianyue , Dong Zhihui 
Published: December 2023

Abstract
Background
Angiitis-induced critical limb ischemia (AICLI), which is defined as critical limb ischemia (CLI) caused by thromboangiitis obliterans (TAO) or other arteritis-related autoimmunological diseases. In the current study, we aimed to report the 10-year outcomes of AICLI patients who underwent purified CD34+ cells (PCCs) transplantation.
Methods
AICLI patients who underwent PCCs transplantation at our center from May 2009 to September 2011 were retrospectively enrolled. The main outcome was major amputation-free survival (MAFS); other outcomes included Rutherford classification, pain-free walking time (PFWT), Wong-Baker Faces Pain Rating Scale (WBFPS), recurrence, new lesions, quality of life (QoL) and patients’ posttransplantation work conditions.
Results
Twenty-four patients were enrolled with a mean age of 41.5 ± 7.8 years. Three underwent major amputation during the follow-up, and the 10-year MAFS was 87.5%. Eight were observed to have recurrence, and 2 had new lesions; the 10-year recurrence-free rate was 66.1%. All patients were unable to work at admission, 17 (70.8%) patients were reemployed after transplantation.
Conclusion
The current study further demonstrated satisfactory long-term efficacy of PCCs transplantation, with a 10-year MAFS of 87.5%. However, the 10-year recurrence-free rate of 66.1% suggested that strict and regular long-term follow-up is necessary.
Cognitive Control in Heroin Use Disorder Patients after Prolonged Methadone Maintenance Treatment
Authors: Zhao Wei , Duan Fan , Li Xiangyu , Li Junda , Xia Lingling , Ren Zixuan , Song Li , Song Peipei , Mu Linlin , Wang Lijin , Zhang Jing , Song Xun , Wang Ze , Chen Jinxuan , Zhang Xiaochu , Jiao Dongliang 
Published: December 2023

Abstract

Objective
Relapse after detoxification remains a major challenge for addiction treatment worldwide. Although cognitive control impairment is common in heroin use disorder (HUD) patients and is considered a major cause of relapse, it remains unclear after long-term detoxification. The aim of the present study was to assess cognitive control and its association with relapse in HUD patients after prolonged periods of drug abstinence.
Methods
A total of 63 HUD patients (mean abstinence: 12.24 ± 2.92 years), including 41 relapsers and 22 non-relapsers, and 31 healthy volunteers participated in this study. The eye tracking tasks, prospective memory tasks, Behavior Rating Inventory of Executive Function-Adult Version (BRIEF-A) and Prospective and Retrospective Memory Questionnaire (PRMQ) were used to assess cognitive control.
Results
HUD patients showed worse saccade error rate but no significant impairment in prospective memory. Furthermore, compared to the non-relapsers, the relapsers performed worse in BRIEF-A scores, antisaccade amplitude and velocity. Moreover, the antisaccade amplitude and velocity were negatively correlated with the BRIEF-A subtest scores. Finally, impairment of inhibition, shift and self-monitoring mediated the association between relapse and poor antisaccade performance.
Conclusions
Even after prolonged methadone treatment, heroin relapse is associated with worse executive function and antisaccade, and antisaccade amplitude and velocity mainly reflect impairment of inhibition, shift and self-monitoring in executive function.
Change in tissue resistance after irreversible electroporation in liver tumors as an indicator of treatment success - A multi-center analysis with long term follow-up
Authors: Frühling Petter , Stillström David , Holmquist Fredrik , Nilsson Anders , Freedman Jacob 
Published: December 2023

Abstract

We examined the correlation between decrease in tissue resistance and time to local tumor recurrence after irreversible electroporation (IRE) in patients with hepatocellular carcinoma (HCC) and colorectal cancer liver metastases (CRCLM). This population-based retrospective cohort study included consecutive patients treated with IRE for liver tumors in Sweden betweem 2011 and 2018. In total 149 patients with 206 tumors were treated. Exclusion due to missing and inaccurate data resulted in 124 patients with 170 tumors for the analyses. In a multivariable Cox regression model, a smaller decrease in tissue resistance (HR 1.02 (1.01-1.03), p=0.002) and tumors of size 21-30 mm (HR 2.48, 95% CI 1.26-4.88, p=0.009) and >30mm (HR 2.56, 95% CI 1.17-5.71, p= 0.019) were associated with shorter time to local tumor recurrence for CRCLM. This correlation was not seen for HCC. We found an association between a decrease in tissue resistance and time to local tumor recurrence for CRCLM. Our findings suggest that a decrease in tissue resistance, instead of rise in current, may be the parameter the interventionist should use during IRE to decide if the treatment is successful.
Detection of Coronavirus in Viral Transport Media using Ultraviolet and Near-Infrared Absorbance Spectra and Pattern Recognition Model
Authors: Rumaling Muhammad Izzuddin , Chee Fuei Pien , Bade Abdullah , Chang Jackson Hian Wui , Goh Lucky Poh Wah , Juhim Floressy 
Published: December 2023

Abstract
SARS-CoV-2 causes individuals to become infected with respiratory disease known as COVID-19. Rapid and robust identification ensures that the infected patients can be quarantined. In this paper, the detection of SARS-CoV-2 utilizes ultraviolet (UV) and near-infrared (NIR) absorbance spectra, along with principal component analysis and linear discriminant analysis (PCA-LDA). A total of 75 negative and 75 positive swab samples are separately placed in vials of viral transport media and transferred into cuvettes. The absorbance spectra are acquired and processed before they undergo dimensionality reduction using PCA. The dataset is divided into training set and testing set to develop and evaluate the PCA-LDA model. The scree plot analysis reveals that the two principal components are optimal for both UV and IR absorbance spectra. By utilizing the first two principal components, the performance indicators demonstrate higher accuracy (97.00%), sensitivity (94.84%), and specificity (99.31%) on IR absorbance spectra. This is attributed to the overall difference in IR absorbance, as well as two peaks centred at 558.5 nm and 972 nm respectively. Utilizing IR absorbance spectra with PCA-LDA model is cost-effective while showing performance comparable to conventional methods such as polymerase chain reaction. This method provides an alternative for rapid and effective SARS-CoV-2 detection.