Flow intermittency affects the nutritional quality of phototrophic biofilms and their capacity to support secondary production
Authors: Courcoul Camille , Boulêtreau Stéphanie , Bec Alexandre , Danger Michael , Felten Vincent , Pradalier Cédric , Roche‐Bril Mathilde , Leflaive Joséphine 
Published: October 2023

Abstract

In streams, phototrophic biofilms are considered to be a good‐quality resource for consumers and are essential to support secondary production. However, with the increasing occurrence of flow intermittency as a consequence of global climate change, limited information exists regarding the impact of drying and rewetting events on biofilm nutritional quality indicators and their consequences for consumers. This study aims at understanding how river intermittency affects the nutritional quality of phototrophic biofilms. Specifically, we examine the effects of drying and rewetting events on their capacity to support secondary production.

Our hypothesis was that the capacity of biofilms to support secondary production relies on their nutritional quality: biofilms characterised by higher contents of long‐chain fatty acids, nitrogen and phosphorus are expected to provide a better‐quality resource for consumers. We also hypothesised that the nutritional quality of biofilms undergoes changes over time during drying events, and that these changes are influenced by their initial algal composition. This is because the algal composition within biofilms may shift in response to drying events, subsequently impacting the nutritional quality of the biofilms.

We grew four phototrophic biofilms in flowing water, each with a different nutritional quality, and then exposed them to a short (3 days) or a long dry period (14 days). Biofilms were sampled 3 days and 18 days after rewetting (post‐disturbance and post‐recovery) to assess alterations in nutritional indicators relative to their pre‐disturbance state through pigment, fatty acid and stoichiometric analyses. We fed these biofilms to Gammarids (Gammarus fossarum) for 29 days and measured individual growth, feeding rate and locomotor activity. We also calculated a secondary production index to assess the biofilms' capacity to support higher trophic levels.

Our findings revealed that the nutritional quality of biofilms was significantly reduced during the post‐disturbance phase. The duration of the dry period had minimal effect on this decline. Subsequently, during the recovery phase, nutritional quality indicators improved for biofilms initially dominated by cyanobacteria, while they either remained unchanged or decreased for biofilms initially dominated by diatoms, in comparison to the pre‐disturbance state. As a result, biofilms that initially exhibited a high nutritional quality were disrupted by the dry period, depending on the duration. However, the overall effects of dry period on gammarid's response and on secondary production were less pronounced, which is likely to have resulted from changes in the quantity of available resources.

Our study demonstrates that a disturbance can modify the expected and effective qualities of biofilms. It highlights that biochemical parameters cannot reliably predict biofilm capacity to support secondary production. Biofilm history of disturbance, among other parameters, must be taken into account.