bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet C3G: a key player in vesicle exocytosis, spreading and clot retraction
Authors: Fernández-Infante Cristina , Hernández-Cano Luis , Herranz Óscar , Berrocal Pablo , Sicilia-Navarro Carmen , González-Porras José Ramón , Bastida José María , Porras Almudena , Guerrero Carmen 
Published: February 2024

AbstractC3G is a Rap1 GEF that plays a pivotal role in platelet-mediated processes such as angiogenesis, tumor growth, and metastasis by modulating the platelet secretome. Here, we explore the mechanisms through which C3G governs platelet secretion. For this, we utilized animal models featuring either overexpression or deletion of C3G in platelets, as well as PC12 cell clones expressing C3G mutants. We found that C3G specifically regulates α-granule secretion via PKCδ, but it does not affect δ-granules or lysosomes. C3G activated RalA through a GEF-dependent mechanism, facilitating vesicle docking, while interfering with the formation of the trans-SNARE complex, thereby restricting vesicle fusion. Furthermore, C3G promotes the formation of lamellipodia during platelet spreading on specific substrates by enhancing actin polymerization via Src and Rac1-Arp2/3 pathways, but not Rap1. Consequently, C3G deletion in platelets favored kiss-and-run exocytosis. C3G also controlled granule secretion in PC12 cells, including pore formation. Additionally, C3G-deficient platelets exhibited reduced phosphatidylserine exposure, resulting in decreased thrombin generation, which along with defective actin polymerization and spreading, led to impaired clot retraction. In summary, platelet C3G plays a dual role by facilitating platelet spreading and clot retraction through the promotion of outside-in signaling while concurrently downregulating α-granule secretion by restricting granule fusion.
Zeitlich begrenzter Therapieversuch („time-limited trial“, TLT) auf der Intensivstation
Authors: Jöbges Susanne , Seidlein Anna-Henrikje , Knochel Kathrin , Michalsen Andrej , Duttge Gunnar , Supady Alexander , Dutzmann Jochen , Meier Stefan , Barndt Iris , Neitzke Gerald , Nauck Friedemann , Rogge Annette , Janssens Uwe 
Published: February 2024

ZusammenfassungDie Zunahme der intensivmedizinischen Behandlungsverfahren ist mit einer Steigerung der Komplexität der Entscheidungen über Auswahl, Anwendung und Dauer der Therapiemaßnahmen verbunden. Ob im individuellen Fall ein Behandlungsziel erstrebenswert und der dafür notwendige Behandlungsweg akzeptabel ist, hängt von den persönlichen Wünschen, Werthaltungen und Lebensentwürfen der Patientinnen und Patienten ab. Häufig bestehen Unsicherheiten darüber, ob ein patientenzentriertes Behandlungsziel erreicht werden kann. Der Einsatz eines zeitlich begrenzten Therapieversuches (TLT) als eine verbindliche Übereinkunft zwischen dem Behandlungsteam und der Patientin/dem Patienten bzw. dem juristischen Stellvertretenden zu einem Behandlungskonzept über einen definierten Zeitraum auf der Intensivstation kann hilfreich sein, um Unsicherheiten zu reduzieren und die Fortführung intensivmedizinischer Maßnahmen im Sinne des/der Patient/in besser einordnen und rechtfertigen zu können.
Deepening the synergistic role of additive manufacturing and computational strategies in jewellery
Authors: Cerrato Noemi , Gariboldi Elisabetta , Ferraro Michela , Candidori Sara , Graziosi Serena 
Published: February 2024

AbstractThis study investigates the synergy between additive manufacturing (AM) technologies and computational design strategies in jewellery and how that synergy can be successfully exploited to extend innovation in that field further. A case study called Ecdysis, a bioinspired jewellery collection, is presented. A dedicated computational algorithm has been developed and is described in detail. This algorithm allows for the exploitation of the shape and functional complexity dimensions allowed by AM and the control of the printability of the generated concept. Shape and functional complexity are exploited to mimic the beauty and dynamism of snakes’ slithering mechanism. At the same time, starting from the developed algorithm, multiple digital models and physical prototypes have been fabricated, leveraging material extrusion, vat photopolymerisation, and powder bed fusion processes. This further development step of the collection thus confirms the versatility of both the proposed approach and AM technologies for jewellery. Therefore, the paper demonstrates how unique wearing experiences can be created and how uniqueness can be simultaneously preserved and democratised in jewellery by deepening the synergy between AM technologies and computational strategies.
The Loewner Energy via the Renormalised Energy of Moving Frames
Authors: Michelat Alexis , Wang Yilin 
Published: February 2024

AbstractWe obtain a new formula for the Loewner energy of Jordan curves on the sphere, which is a Kähler potential for the essentially unique Kähler metric on the Weil–Petersson universal Teichmüller space, as the renormalised energy of moving frames on the two domains of the sphere delimited by the given curve.
A virtual reality data visualization tool for dimensionality reduction methods
Authors: Morales-Vega Juan C. , Raya Laura , Rubio-Sánchez Manuel , Sanchez Alberto 
Published: February 2024

AbstractIn this paper, we present a virtual reality interactive tool for generating and manipulating visualizations for high-dimensional data in a natural and intuitive stereoscopic way. Our tool offers support for a diverse range of dimensionality reduction (DR) algorithms, enabling the transformation of complex data into insightful 2D or 3D representations within an immersive VR environment. The tool also allows users to include annotations with a virtual pen using hand tracking, to assign class labels to the data observations, and to perform simultaneous visualization with other users within the 3D environment to facilitate collaboration.
Enhancement of the bioactive compounds and biological activities of maca (Lepidium meyenii) via solid-state fermentation with Rhizopus oligosporus
Authors: Ryu Kyeong Min , Kim Hayeong , Woo Jiho , Lim Juho , Kang Choon Gil , Kim Seung Wook , Kim Taeyoon , Kim Doman 
Published: February 2024

AbstractMaca (Lepidium meyenii Walp) is renowned for its phytochemicals, including amino acids, saponins, and macamides, confer nutritional and medicinal benefits. This study analyzed the bioactive constituents of maca via solid-state fermentation with Rhizopus oligosporus for 0–15 days. After fermentation, the l-carnitine content reached 157.3 μg/g. A 93% increase in macamide B was recorded after 7-day fermentation. Total flavonoid and saponin contents increased by 88.2% and 110.3%, respectively. The fermentation process significantly enhanced the physicochemical attributes of maca; in particular, its water retention and cholesterol-binding capacities increased by 1.73- and 4.30-fold, respectively, compared with the non-fermented maca. Moreover, fermented maca exhibited stronger antioxidant and α-glucosidase-inhibiting effects than non-fermented maca. Finally, the neuroprotective effect of maca on HT-22 cells increased by 23% after 5-day fermentation. These findings demonstrate the potential of fermented maca as a novel ingredient for foods, beverages, and pharmaceuticals.
The case for metacognitive reflection: a theory integrative review with implications for medical education
Authors: Merkebu Jerusalem , Veen Mario , Hosseini Shera , Varpio Lara 
Published: February 2024

AbstractThe concepts of metacognitive reflection, reflection, and metacognition are distinct but have undergone shifts in meaning as they migrated into medical education. Conceptual clarity is essential to the construction of the knowledge base of medical education and its educational interventions. We conducted a theoretical integrative review across diverse bodies of literature with the goal of understanding what metacognitive reflection is. We searched PubMed, Embase, CINAHL, PsychInfo, and Web of Science databases, including all peer-reviewed research articles and theoretical papers as well as book chapters that addressed the topic, with no limitations for date, language, or location. A total of 733 articles were identified and 87 were chosen after careful review and application of exclusion criteria. The work of conceptually and empirically delineating metacognitive reflection has begun. Contributions have been made to root metacognitive reflection in the concept of metacognition and moving beyond it to engage in cycles of reflection. Other work has underscored its affective component, transformational nature, and contextual factors. Despite this merging of threads to develop a richer conceptualization, a theory of how metacognitive reflection works is elusive. Debates address whether metacognition drives reflection or vice versa. It has also been suggested that learners evolve along on a continuum from thinking, to task-related reflection, to self-reflection, and finally to metacognitive reflection. Based on prior theory and research, as well as the findings of this review, we propose the following conceptualization: Metacognitive reflection involves heightened internal observation, awareness, monitoring, and regulation of our own knowledge, experiences, and emotions by questioning and examining cognition and emotional processes to continually refine and enhance our perspectives and decisions while thoughtfully accounting for context. We argue that metacognitive reflection brings a shift in perspective and can support valuable reconceptualization for lifelong learning.
Re-focusing sampling, design and experimental methods to assess rapid evolution by non-native plant species
Authors: Lucas M. S. , Hensen I. , Barratt C. D. , Callaway R. M. , Durka W. , Lekberg Y. , Nagy D. U. , Onstein R. E. , Shah M. A. , van Dam N. M. , Thoma A. E. , Rosche C. 
Published: February 2024

AbstractEvolution can occur over contemporary timescales, which may be crucial for the invasive success of non-native plant species. Many studies have shown rapid evolution by comparing native and non-native populations in common gardens. However, our understanding of the mechanisms underpinning rapid evolution is still incomplete. Here, we identify the progress, applications, and limitations of studies on rapid evolution of non-native plants with respect to sampling, experimental design and experimental methods. To encompass broad variation within and between the ranges, we recommend sampling across large-scale environmental gradients. We also suggest careful consideration of pitfalls related to the choice of seed families and of the biotic interaction under focus. The latter should be chosen with a view on both the experimental treatment and the corresponding field data to estimate population history. Furthermore, we suggest exploiting multiple omics approaches to address the complexity of biotic interactions, and to account for non-adaptive evolution with molecular data on demographic history of populations. We also reviewed papers that studied rapid evolution in non-native plants and quantified how many of these met our criteria. We anticipate that disentangling adaptive and non-adaptive drivers of among-population variation can increase the accuracy of research on rapid evolution, and that integrating phenotypic, metabolomic and population genomic data can bring opportunities for studying complex biotic interactions. We also illustrate the importance of large collaborative networks and present our scientific network iCONNECT (integrative CONyza NEtwork for Contemporary Trait evolution), with the goal of motivating similar studies on the mechanistic understanding of rapid evolution.
The impact of germline BRCA pathogenic variants in locally advanced, triple negative breast cancer treated with platinum-based neoadjuvant chemotherapy
Authors: Mutai Raz , Kuchuk Iryna , Goldshtein Alexandra , Yerushalmi Rinat , Rotem Ofer , Maisel Lotan Adi , Bdolah-Abram Tali , Gabizon Alberto , Goldvaser Hadar 
Published: February 2024

Abstract
Background
Whether germline BRCA (gBRCA) pathogenic variants (PV) affect prognosis of women with triple negative breast cancer (TNBC) and whether it has implications for treatment decisions in the neoadjuvant setting is unclear.

Methods
This is a retrospective two-center cohort study comprising all women with early stage TNBC who have completed genetic testing and were treated with neoadjuvant dose-dense doxorubicin and cyclophosphamide followed by paclitaxel and carboplatin. All eligible patients treated between 10.2014 and 3.2020 were included. Data on clinico-pathological, pathological response, overall survival (OS) and disease-free survival (DFS) were evaluated. Differences in clinico-pathological features and outcomes were analyzed according to gBRCA status.

Results
Sixty-four women were included in the final analysis, of which 31 had gBRCA PV (gBRCA carriers) and 33 were gBRCA wild-type. Clinico-pathological characteristics were similar between both groups. The odds for pathological complete response (pCR) were significantly higher in gBRCA carriers (74.2%) compared to BRCA wild-type women (48.5%), p = 0.035. At a median follow-up of 30 months, gBRCA carriers had significantly favorable OS (HR = 8.64, 95% CI 1.08–69.21, p = 0.042). The difference in DFS did not reach statistical significance (HR = 7.4, 95% CI 0.91–60.27, p = 0.062). The favorable OS for gBRCA carriers remained significant in multivariate analysis (p = 0.029) and was noted regardless of pathological response (p = 0.018).

Conclusion
Compared to wild-type, gBRCA carriers with locally advanced TNBC treated with neoadjuvant chemotherapy containing carboplatin had a higher pCR rate and better outcomes. These results strengthen the contention that gBRCA status should be considered when tailoring treatment decisions in women with locally advanced TNBC.
PD-1 inhibitor combined with albumin paclitaxel and apatinib as second-line treatment for patients with metastatic gastric cancer: a single-center, single-arm, phase II study
Authors: Gou Miaomiao , Zhang Yong , Wang Zhikuan , Qian Niansong , Dai Guanghai 
Published: February 2024

Summary
Background
Immune checkpoint inhibitors have been approved for first- and third-line treatment of advanced gastric cancer. However, pembrolizumab alone in the second line did not improve overall survival compared to chemotherapy in the KEYNOTE-061 study. In this study, we aimed to explore the efficacy and safety of a three-drug regimen of PD-1 inhibitor combined with albumin paclitaxel and apatinib (a VEGFR inhibitor) for the second-line treatment of patients with metastatic gastric cancer (mGC).

Methods
This was a single-center, single-arm, phase II clinical study. Patients with mGC with stable microsatellite and negative HER-2 expression who failed first-line chemotherapy were enrolled. The enrolled patients were treated with PD-1 inhibitor (selected according to patients’ requirements) in combination with albumin paclitaxel (125 mg/m2, intravenously, days 1 and 8, or 250 mg/m2, intravenously, day 1) and apatinib (250 or 500 mg, orally, days 1–21) every 3 weeks. The primary endpoint was progression-free survival (PFS), and the secondary endpoints were overall survival (OS), objective response rate (ORR), disease control rate (DCR), duration of response, and adverse events (AEs).

Results
From July 11, 2019, to October 13, 2022, a total of 43 patients were enrolled, of whom 10 were PD-L1 negative, 11 were PD-L1 positive, and 22 had unknown PD-L1 expression. As of the data cutoff on April 1st, 2023, nine patients had partial response, 29 had stable disease, and five experienced progressive disease, with the ORR of 20.9% and DCR of 88.3%. The median PFS was 6.2 months (95% CI, 3.9–9.3), and the median OS was 10.1 months (95% CI, 7.5–14.1). All patients suffered from alopecia and neurotoxicity. The other main AEs of grade 1 or 2 were bone marrow suppression (N = 21, 48.8%), hand-foot reaction (N = 19, 44.2%), hypertension (N = 18, 41.9%), hypothyroidism (N = 11, 25.6%), gastrointestinal bleeding (N = 3, 7.0%), and liver function damage (N = 5, 11.6%). Two patients reported grade 3–4 immune-related liver damage.

Conclusion
Second-line PD-1 inhibitor combined with albumin paclitaxel and apatinib showed certain efficacy and safety in patients with mGC.

Trial registration
Clinical trials, NCT04182724. Registered 27 November 2019; retrospectively registered, https://clinicaltrials.gov/study/NCT04182724
Modelling primary school teachers’ acceptance of distance-based educational technologies: A post-pandemic perspective
Authors: Altan Bilge Aslan , Yorulmaz Alper , Karalar Halit 
Published: February 2024

AbstractDistance-based technologies have been one of the foci of technology-related studies since the pandemic first appeared, but there is still a need for further research to gain a greater understanding of how teachers are using these technologies in the post-pandemic era. Thus, the aim of this study is to determine whether the level of digital competence (DC) and techno-pedagogical content knowledge (TPACK) influence the acceptance, by primary school teachers, of distance-based technologies in their post-pandemic teaching. To achieve this, we integrated two external variables, DC and TPACK, into the predictors of the technology acceptance model (TAM), and introduced an extended version of the TAM. The statistics were collected through an online survey of 459 primary school teachers working in various regions of Türkiye. The analysis employed structural equation modelling. The findings suggest that there are statistically significant direct effects of digital competence and perceived usefulness on primary school teachers’ intentions to use distance-based technologies. The extended model explains the connection among the variables, and the findings offer important insights for collaborators in education to better empower teachers to integrate technology favourably and to maintain distance-based technology use in the future.
Club convergence of sustainable development: fresh evidence from developing and developed countries
Authors: Eleftheriou Konstantinos , Nijkamp Peter , Polemis Michael L. 
Published: February 2024

AbstractSustainability is a process that characterizes in a broad sense a nation’s ecological performance and may display a time-varying pattern. Such dynamic trajectories may vary among different countries and prompt not only intriguing questions on space–time convergence but also on the possibility of club convergence. The scope of this study is to investigate the long-run convergence pattern of 137 countries, as presented by their sustainable development index (SDI) over the period 1990–2019. The statistical–econometric analysis used to identify convergence across (groups of) countries is based on the advanced Phillips and Sul (JAE 24:1153–1185, 2009; ECTA 75:1771–1855, 2007) method. The empirical findings from our study allow us to identify two SDI convergence clubs of countries. The first and the biggest club includes mainly the developing African and Asian countries; whereas, the second club includes many OECD countries including inter alia the US, Canada, and Australia. Our analysis brings to light that the transition paths of these two clubs show a significant divergence pattern; this a-symmetry calls also into question the effectiveness of global green policies, such as the clean development mechanism as foreseen in the Kyoto protocol.
Evaluation of lettuce germplasm for resistance to impatiens necrotic spot virus
Authors: Richardson Kelley L. , Nayak Santosh , Hasegawa Daniel K. , Eriksen Renée L. 
Published: February 2024

AbstractRecently, impatiens necrotic spot virus (INSV) has emerged as a major pathogen of lettuce in the Salinas Valley of California. The virus is a member of the genus Orthotospovirus, and under natural conditions, is transmitted by the tiny insect, western flower thrips. Due to the high efficiency of virus transmission, chemical management of thrips vectors is often ineffective in preventing the spread of the disease. Utilization of genetic resistance will be a critical component for managing the disease; however, little is known about genetic sources of resistance in lettuce. Here, we evaluated 189 lettuce lines under natural disease pressure in four field experiments to identify INSV resistant germplasm. The stability of resistance was also evaluated using different inoculation methods under controlled conditions. Our results showed a significant variation (p < 0.0001) among lettuce cultivars for both INSV severity and incidence. We identified INSV resistant germplasm that belongs to different horticultural types and leaf color, including a red-leaf cultivar ‘Eruption’ with a high degree of genetic stability. Moreover, we characterized several factors influencing thrips and host plant relationships, including horticultural type, leaf color, and polyphenol concentration. Overall, INSV resistant germplasm identified in this study provide valuable resources for breeding of INSV resistant lettuce cultivars.
Developing Relational Leaders Through Sorority Engagement: A Quantitative Approach
Authors: Selznick Benjamin S. , Goodman Michael A. , McCready Adam M. , Duran Antonio 
Published: February 2024

AbstractThe purpose of this study was to understand what personal, environmental, and experiential aspects of undergraduate sorority engagement promote relational leadership development. A robust literature review and theories of relational leadership developed for application within the collegiate context provided the foundation for this inquiry. Through use of multilevel modeling, we analyzed a sample of 8,435 undergraduate National Panhellenic Conference sorority women from 172 institutions located within the United States. Results demonstrated the substantial importance of supportive sisterhood at both the individual and group level and showcased how variations in perceptions of sisterhood (e.g., accountability), student involvement (e.g., additional activities), and background characteristics (e.g., SES) affected undergraduate sorority members’ relational leadership development. We close by discussing our findings and offering implications for future practice and research.
Adult Children’s Migration and Health-Related Quality of Life Among Older Nepali Adults
Authors: Ghimire Saruna , Singh Devendra Raj , McLaughlin Sara J. , Nath Dhirendra , McCarren Hannah , Subedi Janardan 
Published: February 2024

AbstractTraditionally, adult children have served as primary caretakers and providers for older Nepali adults. However, out-migration of adult children for employment and other opportunities is increasing. Health-related quality of life (HRQOL) in older Nepali adults in general and in the context of adult children’s migration is poorly understood. This study aims to assess HRQOL of older Nepali adults and its relationship with adult children’s migration. We used existing cross-sectional survey data on 260 older adults from Krishnapur municipality, which has witnessed a high rate of adult migration. HRQOL, quantified using the SF-12 scale, is expressed in terms of a physical (PCS) and mental (MCS) health component. A higher PCS and MCS score, each ranging from 0 to 100, indicates better physical and mental health, respectively. The correlates of HRQOL were assessed in simple and multiple linear regression. Participants had suboptimal HRQOL [mean (± SD): PCS = 40.4 ± 9.2 and MCS = 45.2 ± 7.7]. After adjusting for covariates, adult children’s migration was associated with lower MCS scores (β: -2.33, 95%CI: -4.21, -0.44). Individuals with more than one child had higher MCS scores (β: 2.14, 95%CI: 0.19, 4.09). Females (β: -3.64, 95%CI: -7.21, -0.06) and those with a history of unemployment (β: -6.36, 95%CI: -10.57, -2.15) had lower PCS scores than their respective counterparts. The presence of chronic conditions was associated with significantly lower PCS and MCS scores. Our findings suggest that adult children’s migration may negatively affect HRQOL among older Nepali adults, specifically their psychological well-being. Further research investigating potential moderating factors that may serve as important buffers is needed.