bluuebunny/crossref_metadata_2025_split huggingface dataset:
Field survey of the phase and sex ratios of the brown alga Dictyota dichotoma (Dictyotales, Phaeophyceae) using sex‐specific molecular markers
Authors: Arai Takahiro , Koiwai Keiichiro , Nozaki Reiko , Kondo Hidehiro , Hirono Ikuo , Suzuki Hidekazu , Kamiya Mitsunobu 
Published: January 2024

SUMMARYDominance of one phase (i.e. diploid or haploid) has been reported in various isomorphic macroalgae; however, studies of the relative frequencies of the two phases are limited to a few taxa owing to the difficulty in identifying phases of sterile thalli. In Dictyota dichotoma, we have previously documented the dominance of fertile sporophytes. In the present study, the phase and sex ratios of sterile thalli of D. dichotoma were determined using newly developed sex‐specific markers. Differentially expressed genes were detected by transcriptome analyses of female and male gametophytic strains and a sporophytic strain aiming to identify candidate sex‐specific genes. These candidates were aligned with genome sequences on the sex chromosomes of Ectocarpus siliculosus and were identified as putative sex‐specific genes of D. dichotoma. Two primer sets were designed to discriminate phase and sex by the presence or absence of PCR products, and these markers were applied to examine the phase and sex ratios of D. dichotoma in various habitats and seasons. The proportion of sporophytes against 33–50 thalli examined in each of the five habitats at Ebisu Island, Shizuoka Prefecture was 88–100%, and the proportion of fertile sporophytes against total sporophytes (fertility proportion) varied among the habitats (0–54%). The fertile sporophytes were remarkably larger than the sterile sporophytes. At Tateyama, Chiba Prefecture, the dominance of sporophytes occurred in all seasons. The fertility proportion of sporophyte was relatively high from February (62.4%) to May (91.9%) and lower than 23% in other seasons. When artificial substrates were anchored in the middle of the D. dichotoma population, newly recruited thalli were all sporophytes, suggesting infrequent reproduction via tetraspores in nature. Based on these findings, we discuss potential fitness differences between the phases of D. dichotoma.
Impact of manufacturing servitization on total factor productivity under global trade openness
Authors: Wang Hongsen , Fang Shuanxi , Guo Qing , Meng Bo 
Published: January 2024

AbstractThis paper examines the impact of manufacturing servitization, service trade openness, and their interaction on the total factor productivity (TFP) of the manufacturing industry. Using panel data covering 43 economies, the study finds that higher levels of manufacturing servitization and service trade openness lead to higher TFP in the manufacturing industry. In addition, the positive impact of manufacturing servitization on TFP is greater in economies with higher levels of service trade openness. The study also shows that trade creation and research and development innovation mediate the relationship between manufacturing servitization, service trade openness, and TFP. Finally, the paper finds that the positive impact of manufacturing servitization, service trade openness, and their interaction on TFP is greater in developing countries and in low‐ and high‐TFP manufacturing industries. This research contributes to understanding the intricate relationship between manufacturing servitization, service trade openness, and manufacturing TFP.
A rare vascular injury in a blood donor after whole blood donation
Authors: Scuracchio Patricia , Achkar Ruth , Dias Lara , Oliveira Melina , Casella Ivan , Presti Calogero , Fachini Roberta , Wendel Silvano 
Published: January 2024

AbstractBackgroundBlood donation is a safe process though reactions may still occur. We describe a rare vascular complication in a frequent donor, with improvements in the collection process aimed at avoiding future events.MethodsA 63‐year‐old woman presented with local pain and an apparent collection in the left arm 8 days after donation. Duplex ultrasound identified a superficial liquid collection and signs of arteriovenous fistula (AVF) between the cubital vein and an arterial branch. A computed tomography (CT)‐angio performed 1 day after ultrasound did not identify signs of AVF, followed by a new duplex which confirmed CT‐angio findings. It was assumed that a traumatic AVF evolved with spontaneous thrombosis. In the early follow‐up (18 days), a progressive regression of hematoma was observed without any sequelae.ResultsInvestigation showed a faster whole blood bag collection time (3 min; normal: 5–9 min), and the processed packed red blood cell had a brighter red color than usual. The donor reported local bleeding after needle withdrawal, not observed in previous donations and a bruise forming on the same day. No arterial puncture (AP) was noticed by the collection staff during the procedure. The staff was retrained and actions were taken focusing on more active surveillance of late reactions, highlighting the importance of post‐donation information by the donors, regardless of any adverse reaction observed, to detect late complications.ConclusionWe described an uncommon AP in a donor that was not identified, leading to an AVF that spontaneously thrombosed.
Efficiency Tests of Turbine Water Wheels
Authors: Webber Wm. O. 
Published: January 2024

Abstract
These are records of a series of consecutive tests under uniform conditions of four water wheels, with the same apparatus and the same observers, to determine the efficiency of the wheels, and ascertain the effects of corrosion and wear upon the relative efficiency of the wheels, also to determine whether more modern wheels would show greater relative efficiencies, and whether it would be more economical to operate all of the wheels at part gate all of the time, or some of the wheels at full gate part of the time.
The tests are instructive because of the wide range of speeds and gate openings covered systematically and consecutively. They show conclusively that water wheels should be run at the speed for which they are designed under any given head, that modern wheels, with coarser buckets having a spoon-shaped discharge depending below the guide ring of the wheel, and with the outer periphery of the bucket at discharge of greater diameter than the bucket at entrance, would give much more power, higher speed, and greater efficiencies from the same water than from wheels of older design without these modern improvements.
They also show how rapidly a complete series of efficiency tests of a wheel can be made with properly designed apparatus efficiently handled by a corps of intelligent observers.
Sub-minimum inhibitory concentrations (sub-MICs) of colistin on
Acinetobacter baumannii
biofilm formation potency, adherence, and invasion to epithelial host cells: an experimental study in an Iranian children’s referral hospital
Authors: Yousefi Nojookambari Neda , Eslami Gita , Sadredinamin Mehrzad , Vaezjalali Maryam , Nikmanesh Bahram , Dehbanipour Razieh , Yazdansetad Sajjad , Ghalavand Zohreh 
Published: January 2024

ABSTRACT

Here, we described the efficacy of colistin sub-minimum inhibitory concentrations (sub-MICs) on biofilm-forming activity, host epithelial cell adherence, and invasion capacity of
Acinetobacter baumannii
strains collected from children admitted to the Children’s Medical Center Hospital. Biofilm formation potency of
A. baumannii
clinical isolates was measured using a 96-well microtiter plate assay. Distribution of biofilm-related genes, including
bap
,
abaI
,
ompA
,
csuE
, and
bla
PER-1
, was detected by PCR. The mRNA expression level of
ompA
and
csuE
was measured by qPCR in the presence of ¼ and ½ MICs of colistin.
A. baumannii
adhesion and invasion to eukaryotic host cells were phenotypically assayed at sub-MICs of colistin. Eighty percent (56/70) and 35.7% (25/70) of
A. baumannii
isolates were multidrug-resistant (MDR) and extensively drug-resistant (XDR) phenotypes, respectively. The strong, moderate, and weak biofilm producers of
A. baumannii
were 37.1% (26/70), 32.8%, (23/70), and 22.8% (16/70), respectively. The frequencies of biofilm-associated genes were 100% for
abaI
,
ompA
, and
csuE
, followed by 22.8% (16/70) and 24.3% (17/70) for
bap
and
bla
PER-1
, respectively. The downregulation of
csuE
and
ompA
expression levels was observed in the sub-MIC of colistin.
In vitro
cell culture study showed a decreased capability of
A. baumannii
to adhere to the human epithelial cells at sub-inhibitory doses of colistin; however, none of the isolates could invade HEp-2 cells. Our study showed that the genes encoding biofilm-associated proteins undergo downregulation in expression levels after exposure to sub-MICs of colistin in
A. baumannii
. Longitudinal
in vivo
studies are needed to fully understand the clinical aspects of pathogenicity mechanisms and evolutionary dynamics of drug resistance.

IMPORTANCE

Since the toxicity of colistin is dose dependent, there is a focus on strategies that reduce the dose while maintaining the therapeutic effect of the drug. Our findings about sub-inhibitory doses of colistin provide a novel insight into the logical use of colistin to treat and control
Acinetobacter baumannii
-related infections in clinical practice.
Supplemental Material: Structure and provenance of the Cretaceous Pingshanhu Basin in the Hexi Corridor: Implications for Mesozoic tectonics in the northern Tibetan Plateau
Authors: Wu Chen , al. et , Liu Wenyou 
Published: January 2024

<p>Table S1: Detailed results of detrital zircon U-Pb analyses for sandstone samples from Pingshanhu Basin. Table S2: Summary of geochronology results for magmatic rocks in the Qilian Shan and the Alxa block. </p>
Generalized second law of thermodynamics in massive gravity
Authors: Beigmohammadi Mohammad , Karami Kayoomars 
Published: January 2024

AbstractHere, we study the generalized second law (GSL) of thermodynamics in the framework of massive gravity. To do this, we consider a FRW universe filled only with matter and enclosed by the apparent horizon. In addition, we consider two models including generalized massive gravity (GMG) as well as dRGT massive gravity on de Sitter. For both models, we first study the dynamics of background cosmology and then explore the validity of GSL. We conclude that for the selected values of model parameters the GSL is respected.
A articulação entre o trabalho e a educação de uma imigrante órfã
Authors: Negrão Ana Maria Melo 
Published: January 2024

Esta inquietação sobre a trajetória de uma órfã imigrante espanhola, a Tata, motivou a pesquisa de meu doutorado na Faculdade de Educação da UNICAMP. Buscou reconstruir os fatores sociais, a favoreceram a trajetória da órfã, em Campinas, desde pequena, dedicada ao trabalho não remunerado em uma única casa de família, sem ser adotada, sem frequentar escola regular e como isso se refletia no imaginário social em uma cidade elitista, nas décadas de 20 a 90 do século XX. A fonte utilizada, além de poucos documentos, fotografias, foi a história oral, com relatos de três gerações cuidadas por ela, da mesma família, ou de amigas e pessoas que a conheceram, com informações a completar o que as fontes escritas silenciavam. As pessoas cederam sua voz, despertando lembranças, fatos e histórias de uma vida toda dedicada ao trabalho.
Ruptured abdominal aortic aneurysm discovered by pocket-sized ultrasound in a low resource setting: a case report
Authors: Alasasfeh Ihab , Abudawood Rawan , E.Hwidi Bayan , Al-Shami Raghad 
Published: January 2024

Abstract
Background
Abdominal aortic aneurysm (AAA) is a life-threatening condition characterized by the weakening and dilation of the abdominal aorta. AAA primarily affects men, smokers, and the elderly, with rupture being a fatal complication. While point-of-care ultrasound (POCUS) is valuable in diagnosing AAA, the role of using pocket-sized ultrasound in a low resource setting remains less explored. This case report presents a unique instance of a suspected ruptured AAA diagnosed using pocket-sized ultrasound in an emergency department (ED) situated in a low resource setting where ultrasound machines are absent, and emergency physicians lack proficiency in ultrasound usage.

Case presentation
A 78-year-old man with a history of hypertension and bladder cancer presented to the ED with suprapubic pain. Initial evaluation showed no concerning findings. However, the next day, he collapsed, became unconscious, and experienced a cardiac arrest. Despite resuscitation efforts, the patient’s condition deteriorated. POCUS revealed an 8-cm dilated abdominal aorta with an intimal flap, indicative of a dissecting AAA and a substrate for AAA rupture. Unfortunately, the patient died despite resuscitation efforts.

Conclusion
This case highlights the importance of considering AAA in patients with risk factors and abdominal pain in a low resource setting. POCUS using a pocket-sized ultrasound can aid in early AAA detection, potentially preventing rupture through preemptive vascular intervention. Emergency departments should prioritize ultrasound availability, and emergency physicians should be proficient in its use.
COVID-19 detection from chest X-ray images using CLAHE-YCrCb, LBP, and machine learning algorithms
Authors: Prince Rukundo , Niu Zhendong , Khan Zahid Younas , Emmanuel Masabo , Patrick Niyishaka 
Published: January 2024

Abstract
Background
COVID-19 is a disease that caused a contagious respiratory ailment that killed and infected hundreds of millions. It is necessary to develop a computer-based tool that is fast, precise, and inexpensive to detect COVID-19 efficiently. Recent studies revealed that machine learning and deep learning models accurately detect COVID-19 using chest X-ray (CXR) images. However, they exhibit notable limitations, such as a large amount of data to train, larger feature vector sizes, enormous trainable parameters, expensive computational resources (GPUs), and longer run-time.

Results
In this study, we proposed a new approach to address some of the above-mentioned limitations. The proposed model involves the following steps: First, we use contrast limited adaptive histogram equalization (CLAHE) to enhance the contrast of CXR images. The resulting images are converted from CLAHE to YCrCb color space. We estimate reflectance from chrominance using the Illumination–Reflectance model. Finally, we use a normalized local binary patterns histogram generated from reflectance (Cr) and YCb as the classification feature vector. Decision tree, Naive Bayes, support vector machine, K-nearest neighbor, and logistic regression were used as the classification algorithms. The performance evaluation on the test set indicates that the proposed approach is superior, with accuracy rates of 99.01%, 100%, and 98.46% across three different datasets, respectively. Naive Bayes, a probabilistic machine learning algorithm, emerged as the most resilient.

Conclusion
Our proposed method uses fewer handcrafted features, affordable computational resources, and less runtime than existing state-of-the-art approaches. Emerging nations where radiologists are in short supply can adopt this prototype. We made both coding materials and datasets accessible to the general public for further improvement. Check the manuscript’s availability of the data and materials under the declaration section for access.
Esketamine use is associated with shortened postoperative hospital stay in patients after knee arthroscopic surgery: a propensity score–matched cohort study
Authors: Liu Jing , Han Hong , Yang Shangze , Zhan Xiaoxuan , Cao Bingbing , Peng Yue 
Published: January 2024

Abstract
Background
Previous studies have examined anesthetics to improve postoperative prognosis after knee arthroscopic surgery. However, it is currently unknown whether perioperative anesthetics can influence postoperative hospital stay. We investigated the impact of esketamine after knee arthroscopic surgery on post-operative length of stay, fever and surgical site infection.

Methods
This study included 455 patients who underwent knee surgery between January2020 and August 2021at a tertiary hospital in China. Patient characteristics, preoperative laboratory values, intra-operative anesthetic data, and postoperative outcomes were collected. Univariate and multivariate logistic regression analyses with or without propensity score matching were performed to identify factors related to post-operative discharge within 3 days(PD3).

Results
A total of 297 cases met our inclusion criteria. The mean age of patients was 42 ± 14 years, mean body mass index, 24.1 ± 3.5 kg/m2, 157(53%) patients were male. Meniscus-related procedures accounted for the most part of all the procedures with a percentage of 40.4%, followed by combined procedures of 35.4%. After we adjusted for demographic and intraoperative characteristics with propensity score matching, esketamine use was significantly associated with PD3 with the highest odds ratio of 2.28 (95% confidence interval (CI): 1.18–4.41, p = 0.014).

Conclusion
Esketamine use was associated with PD3 in patients underwent knee arthroscopic surgery. The findings of this study will be useful to anesthesiologists in making informed decisions regarding the choice of anesthetics for knee joint diseases.

Trial registration
This study was approved by the Ethics Committee (Approval No.:2023-041-01) of the Eighth Affiliated Hospital, Sun Yat-sen University and retrospectively registered.
The effectiveness of allied health and nurse practitioner models-of-care in managing musculoskeletal conditions in the emergency department: a systematic review and meta-analysis
Authors: Vella Simon P , Melman Alla , Coombs Danielle , Maher Christopher G , Swain Michael S , Monk Elizabeth , Machado Gustavo C 
Published: January 2024

Abstract
Background
Musculoskeletal conditions are the most common health condition seen in emergency departments. Hence, the most effective approaches to managing these conditions is of interest. This systematic review aimed to evaluate the effectiveness of allied health and nursing models of care for the management of musculoskeletal pain in ED.

Methods
MEDLINE, EMBASE, CINAHL and LILACS databases were searched from inception to March 2023 for published randomised trials that compared the effectiveness of allied health and nursing models of care for musculoskeletal conditions in ED to usual ED care. Trials were eligible if they enrolled participants presenting to ED with a musculoskeletal condition including low back pain, neck pain, upper or lower limb pain and any soft tissue injury. Trials that included patients with serious pathology (e.g. malignancy, infection or cauda equina syndrome) were excluded. The primary outcome was patient-flow; other outcomes included pain intensity, disability, hospital admission and re-presentation rates, patient satisfaction, medication prescription and adverse events. Two reviewers performed search screening, data extraction, quality and certainty of evidence assessments.

Results
We identified 1746 records and included 5 randomised trials (n = 1512 patients). Only one trial (n = 260) reported on patient-flow. The study provides very-low certainty evidence that a greater proportion of patients were seen within 20 min when seen by a physician (98%) than when seen by a nurse (86%) or physiotherapist (77%). There was no difference in pain intensity and disability between patients managed by ED physicians and those managed by physiotherapists. Evidence was limited regarding patient satisfaction, inpatient admission and ED re-presentation rates, medication prescription and adverse events. The certainty of evidence for secondary outcomes ranged from very-low to low, but generally did not suggest a benefit of one model over another.

Conclusion
There is limited research to judge the effectiveness of allied health and nursing models of care for the management of musculoskeletal conditions in ED. Currently, it is unclear as to whether allied health and nurse practitioners are more effective than ED physicians at managing musculoskeletal conditions in ED. Further high-quality trials investigating the impact of models of care on service and health outcomes are needed.
Seamless phase 2/3 design for trials with multiple co-primary endpoints using Bayesian predictive power
Authors: Yang Jiaying , Li Guochun , Yang Dongqing , Wu Juan , Wang Junqin , Gao Xingsu , Liu Pei 
Published: January 2024

AbstractSeamless phase 2/3 design has become increasingly popular in clinical trials with a single endpoint. Trials that define success based on the achievement of all co-primary endpoints (CPEs) encounter the challenge of inflated type 2 error rates, often leading to an overly large sample size. To tackle this challenge, we introduced a seamless phase 2/3 design strategy that employs Bayesian predictive power (BPP) for futility monitoring and sample size re-estimation at interim analysis. The correlations among multiple CPEs are incorporated using a Dirichlet-multinomial distribution. An alternative approach based on conditional power (CP) was also discussed for comparison. A seamless phase 2/3 vaccine trial employing four binary endpoints under the non-inferior hypothesis serves as an example. Our results spotlight that, in scenarios with relatively small phase 2 sample sizes (e.g., 50 or 100 subjects), the BPP approach either outperforms or matches the CP approach in terms of overall power. Particularly, with n1 = 50 and ρ = 0, BPP showcases an overall power advantage over CP by as much as 8.54%. Furthermore, when the phase 2 stage enrolled more subjects (e.g., 150 or 200), especially with a phase 2 sample size of 200 and ρ = 0, the BPP approach evidences a peak difference of 5.76% in early stop probability over the CP approach, emphasizing its better efficiency in terminating futile trials. It’s noteworthy that both BPP and CP methodologies maintained type 1 error rates under 2.5%. In conclusion, the integration of the Dirichlet-Multinominal model with the BPP approach offers improvement in certain scenarios over the CP approach for seamless phase 2/3 trials with multiple CPEs.