bluuebunny/crossref_metadata_2025_split huggingface dataset:
A systematic literature review of reciprocity in engineering service‐learning/community engagement
Authors: Delaine David A. , Redick Sarah , Radhakrishnan Dhinesh , Shermadou Amena , Smith Mandy McCormick , Kandakatla Rohit , Wang Linjue , Freitas Claudio , Dalton Casey L. , Dostilio Lina Dee , DeBoer Jennifer 
Published: October 2023

AbstractBackgroundScholars agree that reciprocity is a cornerstone of service‐learning and community engagement (SLCE); however, engagement with this concept varies widely in practice and across disciplines. To enhance the potential of SLCE to fulfill its promise for societal impact, engineering education must understand how reciprocity is achieved, recognize barriers that inhibit its progress, and identify strategies for how it can be strengthened.PurposeWe performed this review to understand the ways reciprocity is articulated in the engineering SLCE literature. Drawing from these articulations, we examined the extent of engagement with reciprocity toward providing insights into the design and assessment of SLCE efforts for reciprocity.Scope/MethodWe performed a systematic literature review on engineering SLCE at institutes of higher education. Following an established approach to identify and synthesize articles, we developed deductive codes by distilling three well‐articulated orientations of reciprocity. We then analyzed the operationalization of reciprocity in the literature.ResultsThe literature demonstrated varying degrees of reciprocity. Minimally reciprocal efforts centered university stakeholders. In contrast, highly reciprocal partnerships explicitly addressed the nature of engagement with communities. Findings provide insights into the breadth of practice within reciprocity present in engineering SLCE. Further, analysis suggests that our codes and levels of reciprocity can function as a framework that supports the design and evaluation of reciprocity in SLCE efforts.ConclusionsOur review suggests that to enact more equitable SLCE, researchers and practitioners must intentionally conceptualize reciprocity, translate it into practice, and make visible the ways in which reciprocity is enacted within their SLCE efforts.
Guidelines for the purification and characterization of extracellular vesicles of parasites
Authors: Fernandez‐Becerra Carmen , Xander Patrícia , Alfandari Daniel , Dong George , Aparici‐Herraiz Iris , Rosenhek‐Goldian Irit , Shokouhy Mehrdad , Gualdron‐Lopez Melisa , Lozano Nicholy , Cortes‐Serra Nuria , Karam Paula Abou , Meneghetti Paula , Madeira Rafael Pedro , Porat Ziv , Soares Rodrigo Pedro , Costa Adriana Oliveira , Rafati Sima , da Silva Anabela‐Cordeiro , Santarém Nuno , Fernandez‐Prada Christopher , Ramirez Marcel I. , Bernal Dolores , Marcilla Antonio , Pereira‐Chioccola Vera Lucia , Alves Lysangela Ronalte , Portillo Hernando Del , Regev‐Rudzki Neta , de Almeida Igor Correia , Schenkman Sergio , Olivier Martin , Torrecilhas Ana Claudia 
Published: October 2023

AbstractParasites are responsible for the most neglected tropical diseases, affecting over a billion people worldwide (WHO, 2015) and accounting for billions of cases a year and responsible for several millions of deaths. Research on extracellular vesicles (EVs) has increased in recent years and demonstrated that EVs shed by pathogenic parasites interact with host cells playing an important role in the parasite's survival, such as facilitation of infection, immunomodulation, parasite adaptation to the host environment and the transfer of drug resistance factors. Thus, EVs released by parasites mediate parasite‐parasite and parasite‐host intercellular communication. In addition, they are being explored as biomarkers of asymptomatic infections and disease prognosis after drug treatment. However, most current protocols used for the isolation, size determination, quantification and characterization of molecular cargo of EVs lack greater rigor, standardization, and adequate quality controls to certify the enrichment or purity of the ensuing bioproducts. We are now initiating major guidelines based on the evolution of collective knowledge in recent years. The main points covered in this position paper are methods for the isolation and molecular characterization of EVs obtained from parasite‐infected cell cultures, experimental animals, and patients. The guideline also includes a discussion of suggested protocols and functional assays in host cells
A systematic evaluation on the relationship between hypo‐osmoregulation and hyper‐osmoregulation in decapods of different habitats
Authors: Bozza Deivyson Cattine , Freire Carolina Arruda , Prodocimo Viviane 
Published: October 2023

AbstractDecapods occupy all aquatic, and terrestrial and semi‐terrestrial environments. According to their osmoregulatory capacity, they can be osmoconformers or osmoregulators (hypo or hyperegulators). The goal of this study is to gather data available in the literature for aquatic decapods and verify if the rare hyporegulatory capacity of decapods is associated with hyper‐regulatory capacity. The metric used to quantify osmoregulation was the osmotic capacity (OC), the gradient between external and internal (hemolymph) osmolalities. We employ phylogenetic comparative methods using 83 species of decapods to test the correlation between hyper OC and hypo OC, beyond the ancestral state for osmolality habitat, which was used to reconstruct the colonization route. Our analysis showed a phylogenetic signal for habitat osmolality, hyper OC and hypo OC, suggesting that hyper‐hyporegulators decapods occupy similar habitats and show similar hyper and hyporegulatory capacities. Our findings reveal that all hyper‐hyporegulators decapods (mainly shrimps and crabs) originated in estuarine waters. Hyper OC and hypo OC are correlated in decapods, suggesting correlated evolution. The analysis showed that species which inhabit environments with intense salinity variation such as estuaries, supratidal and mangrove habitats, all undergo selective pressure to acquire efficient hyper‐hyporegulatory mechanisms, aided by low permeabilities. Therefore, hyporegulation can be observed in any colonization route that passes through environments with extreme variations in salinity, such as estuaries or brackish water.
Straightforward method for the synthesis of 2H‐furo[3,2‐b]pyran‐2‐one derivatives starting from allomaltol
Authors: Komogortsev Andrey N. , Lichitsky Boris V. , Milyutin Constantine V. , Melekhina Valeriya G. 
Published: October 2023

AbstractWe developed a facile approach for the preparation of a rare 2H‐furo[3,2‐b]pyran‐2‐one system. The suggested method is based on a two‐step process using unsubstituted allomaltol as a starting compound. The key stage of the considered route is a condensation of 2‐(2‐aryl‐2‐oxoethyl)‐3‐hydroxy‐6‐methyl‐4H‐pyran‐4‐ones with 1,1‐carbonyldiimidazole. The advantages of the presented method are commercially available starting materials and convenient experimental procedures including simple isolation of the target compounds. The structure of one of the 2H‐furo[3,2‐b]pyran‐2‐ones was confirmed by x‐ray diffraction.
Expression patterns of the transcription factors Fezf1, Fezf2, and Bcl11b in the olfactory organs of turtle embryos
Authors: Nakamuta Shoko , Noda Hideki , Kato Hideaki , Yokoyama Takuya , Yamamoto Yoshio , Nakamuta Nobuaki 
Published: October 2023

AbstractMany tetrapod vertebrates have two distinct olfactory organs, the olfactory epithelium (OE) and vomeronasal organ (VNO). In turtles, the olfactory organ consists of two types of sensory epithelia, the upper chamber epithelium (UCE; corresponding to the OE) and the lower chamber epithelium (LCE; corresponding to the VNO). In many turtle species, the UCE contains ciliated olfactory receptor cells (ORCs) and the LCE contains microvillous ORCs. To date, several transcription factors involved in the development of the OE and VNO have been identified in mammals. Fez family zinc‐finger protein 1 and 2 (Fezf1 and 2) are expressed in the OE and VNO, respectively, of mouse embryos, and are involved in the development and maintenance of ORCs. B‐cell lymphoma/leukemia 11B (Bcl11b) is expressed in the mouse embryo OE except the dorsomedial parts of the nasal cavity, and regulates the expression of odorant receptors in the ORCs. In this study, we examined the expression of Fezf1, Fezf2, and Bcl11b in the olfactory organs of embryos in three turtle species, Pelodiscus sinensis, Trachemys scripta elegans, and Centrochelys sulcata, to evaluate their involvement in the development of reptile olfactory organs. In all three turtle species, Bcl11b was expressed in the UCE, Fezf2 in the LCE, and Fezf1 in both the UCE and LCE. These results imply that the roles of the transcription factors Fezf1, Fezf2, and Bcl11b in olfactory organ development are conserved among mammals and turtles.
Accelerated Cine Cardiac MRI Using Deep Learning‐Based Reconstruction: A Systematic Evaluation
Authors: Pednekar Amol , Kocaoglu Murat , Wang Hui , Tanimoto Aki , Tkach Jean A. , Lang Sean , Taylor Michael D. 
Published: October 2023

BackgroundBreath‐holding (BH) for cine balanced steady state free precession (bSSFP) imaging is challenging for patients with impaired BH capacity. Deep learning‐based reconstruction (DLR) of undersampled k‐space promises to shorten BHs while preserving image quality and accuracy of ventricular assessment.PurposeTo perform a systematic evaluation of DLR of cine bSSFP images from undersampled k‐space over a range of acceleration factors.Study TypeRetrospective.SubjectsFifteen pectus excavatum patients (mean age 16.8 ± 5.4 years, 20% female) with normal cardiac anatomy and function and 12‐second BH capability.Field Strength/Sequence1.5‐T, cine bSSFP.AssessmentRetrospective DLR was conducted by applying compressed sensitivity encoding (C‐SENSE) acceleration to systematically undersample fully sampled k‐space cine bSSFP acquisition data over an acceleration/undersampling factor (R) considering a range of 2 to 8. Quality imperceptibility (QI) measures, including structural similarity index measure, were calculated using images reconstructed from fully sampled k‐space as a reference. Image quality, including contrast and edge definition, was evaluated for diagnostic adequacy by three readers with varying levels of experience in cardiac MRI (>4 years, >18 years, and 1 year). Automated DL‐based biventricular segmentation was performed commercially available software by cardiac radiologists with more than 4 years of experience.Statistical TestsTukey box plots, linear mixed effects model, analysis of variance (ANOVA), weighted kappa, Kruskal–Wallis test, and Wilcoxon signed‐rank test were employed as appropriate. A P‐value <0.05 was considered statistically significant.ResultsThere was a significant decrease in the QI values and edge definition scores as R increased. Diagnostically adequate image quality was observed up to R = 5. The effect of R on all biventricular volumetric indices was non‐significant (P = 0.447).Data ConclusionThe biventricular volumetric indices obtained from the reconstruction of fully sampled cine bSSFP acquisitions and DLR of the same k‐space data undersampled by C‐SENSE up to R = 5 may be comparable.Evidence Level3Technical EfficacyStage 1
Nutrition guidelines for preterm infants: A systematic review
Authors: Meiliana Meiliana , Alexander Tanith , Bloomfield Frank H. , Cormack Barbara E. , Harding Jane E. , Walsh Orla , Lin Luling 
Published: October 2023

AbstractBackgroundThere is no consensus on optimal nutrition for preterm infants, leading to substantial practice variation. We aimed to assess the quality of nutrition guidelines for preterm infants, the consistency of recommendations, and the gaps in recommendations.MethodsWe searched databases and websites for nutrition guidelines for preterm infants before first hospital discharge, which were endorsed, prepared, or authorized by a regional, national, or international body, written in English, and published between 2012 and 2023. Two reviewers independently screened articles and extracted the recommendations. Four reviewers appraised the included guidelines using Appraisal of Guidelines, Research, and Evaluation II.ResultsA total of 7051 were identified, with 27 guidelines included, 26% of which were high in quality. Most guidelines lacked stakeholder involvement and rigor of development. We found considerable variation in recommendations, many of which lacked details on certainty of evidence and strength of recommendation. Recommendations for type of feed and breastmilk fortification were consistent among high‐quality guidelines, but recommendations varied for intakes of almost all nutrients and monitoring of nutrition adequacy. Different guidelines gave different certainty of evidence for the same recommendations. Most gaps in recommendations were due to very low certainty of evidence.ConclusionFuture development of nutrition guidelines for preterm infants should follow the standard guideline development method and ensure the rigorous process, including stakeholders' involvement, to improve the reporting of strength of recommendation, certainty of evidence, and gaps in recommendation. Evidence is needed to support recommendations about macro and micronutrient intakes, breastmilk fortification, and markers on adequacy of intake of different nutrients.
Hypersensitivity to glucagon‐like peptide‐1 receptor agonists: A case of delayed urticaria‐like rash to dulaglutide and literature review
Authors: Bianchi Leonardo , Ali Biglu Marash Samane , Malatesta Nicolò , Hansel Katharina , Tramontana Marta , Stingeni Luca 
Published: October 2023

AbstractDulaglutide is a glucagon‐like peptide‐1 (GLP‐1) receptor agonist, which stimulates insulin release via activation of GLP‐1 receptors on pancreatic beta cells. We report the case of a 45‐year‐old woman with type 2 diabetes mellitus who started dulaglutide subcutaneously and developed, a few minutes after the fourth administration, a persistent itchy reaction at the injection site, followed, after two more administrations, by a diffuse itchy delayed urticaria‐like rash, healed in 7 days after drug interruption. Skin test performed after 3 months from the event demonstrated the culprit role of the drug. Given the limited evidence in literature, there is currently no standardised protocol for testing patients sensitised to one or more glucagon‐like peptide‐1 receptor agonists. Performing skin tests, especially intradermal tests, is pivotal to confirm the aetiological role of the drug in the suspected hypersensitivity reaction, and possibly to identify an alternative to be proposed before precluding the entire drug class. To the best of our knowledge, this is the first case of systemic hypersensitivity reaction to dulaglutide presenting as a delayed urticaria‐like rash and confirmed by positive skin test.
Kinetics and mechanism of dissolved organic phosphorus (DOP) digestion by the UV/O3 process
Authors: Zhu Jialong , Fang Kaixuan , Zhang Mengdi , Chen Huixuan , Zhou Mengfei , Sun Xiaofang 
Published: October 2023

AbstractIn this study, the kinetics and mechanism of UV/O3 synergistic oxidative digestion of dissolved organic phosphorus (DOP) were investigated, focusing on the ozone direct oxidation and hydroxyl radical oxidation parts of glufosinate and triphenyl phosphate (TPhP). The p‐chlorobenzoic acid (p‐CBA) was selected as the probe compound, and two kinds of reaction kinetic models were proposed by competitive kinetic method with Rct according to the different scale of rate constants of hydroxyl radical oxidation. Under the condition of weakly alkaline (pH = 9.0) and weakly acidic environment (pH = 5.0), the second‐order rate constants of glufosinate and TPhP was determined indirectly to be ko3/glufosinate = (2.903 ± 0.247)M−1s−1 and ko3/TPhP = (3.307 ± 0.204) M−1s−1 by ozone direct oxidation, and k·OH/glufosinate = (1.257 ± 1.031) × 109 M−1s−1 and k·OH/TPhP = (7.120 × 108 ± 0.963) M−1s−1 by hydroxyl radical oxidation, respectively. The comparison of the contribution levels of the two parts to the digestion process showed that the contribution levels in the digestion of glufosinate and TPhP processes both the contribution of ·OH were higher than those of ozone, 86.3% and 72.6%, respectively.
Hydrology, rather than wildfire burn extent, determines post‐fire organic and black carbon export from mountain rivers in central coastal California
Authors: Barton Riley , Richardson Christina M. , Pae Evelyn , Montalvo Maya S. , Redmond Michael , Zimmer Margaret A. , Wagner Sasha 
Published: October 2023

AbstractCoastal mountain rivers export disproportionately high quantities of terrestrial organic carbon (OC) directly to the ocean, feeding microbial communities and altering coastal ecology. To better predict and mitigate the effects of wildfires on aquatic ecosystems and resources, we must evaluate the relationships between fire, hydrology, and carbon export, particularly in the fire‐prone western United States. This study examined the spatiotemporal export of particulate and dissolved OC (POC and DOC, respectively) and particulate and dissolved black carbon (PBC and DBC, respectively) from five coastal mountain watersheds following the 2020 CZU Lightning Complex Fires (California, USA). Despite high variability in watershed burn extent (20–98%), annual POC, DOC, PBC, and DBC concentrations remained relatively stable among the different watersheds. Instead, they correlated significantly with watershed discharge. Our findings indicate that hydrology, rather than burn extent, is a primary driver of post‐fire carbon export in coastal mountain watersheds.
Spectroscopically dark phosphate features revealed by chemical exchange saturation transfer
Authors: Lu Jiaqi , Straub Joshua S. , Nowotarski Mesopotamia S. , Han Songi , Xu Xiang , Jerschow Alexej 
Published: October 2023

AbstractPhosphate is an essential anion in the human body, comprising approximately 1% of the total body weight, and playing a vital role in metabolism, cell membranes, and bone formation. We have recently provided spectroscopic, microscopic, and computational evidence indicating that phosphates can aggregate much more readily in solution than previously thought. This prior work provided indirect evidence through the observation of unusual
P NMR relaxation and line‐broadening effects with increasing temperature. Here, we show that, under conditions of slow exchange and selective RF saturation, additional features become visible in chemical exchange saturation transfer (CEST) experiments, which appear to be related to the previously reported phosphate clustering. In particular, CEST shows pronounced dips several ppm upfield of the main phosphate resonance at low temperatures, while direct
P spectroscopy does not produce any signals in that range. We study the pH dependence of these new spectroscopic features and present exchange and spectroscopic parameters based on fitting the CEST data. These findings could be of importance in the investigation of phosphate dynamics, especially in the biological milieu.
Complications Associated with Microdebriders in Otolaryngology Procedures from 2011 to 2021: A MAUDE Study
Authors: Magge Hari , Lee Esther , Shaver Timothy B. , Thakkar Punam G. , Singh Ameet 
Published: October 2023

AbstractObjectiveThe microdebrider is a powered surgical instrument that is widely used in the field of otolaryngology. We aim to identify the type and frequency of device malfunctions, patient complications, and subsequent interventions related to the use of microdebriders.Study DesignCross‐sectional analysis.SettingThe US Food and Drug Administration (FDA) 2011 to 2021 Manufacturer and User Facility Device Experience (MAUDE) database.MethodsThe MAUDE database was queried for reports of “microdebrider,” with adverse events selected that pertained to usage in head and neck surgeries from January 1, 2011 to December 31, 2021.ResultsThere were 282 adverse events in 267 individual medical device reports (MDR). Although the majority of the reports did not specify the specific operation, endoscopic sinus surgery was the most common reported procedure (89, 33.3%). The most common cause of device malfunction was due to a broken piece (120, 48.2%) followed by overheating of the microdebrider motor (78, 31.3%). Of the reports which specified patient injury, the most commonly reported was “unintentional tissue damage,” (10, 32.3%).ConclusionThe microdebrider has demonstrated utility within the field of otolaryngology, but is not without risk of malfunction that can cause patient injury. By understanding possible risks of microdebrider usage, including tissue damage, burns, and bleeds caused by device malfunction or operator error, physicians can be better prepared to address complications and educate patients.
Formate dehydrogenase mimic nanoenzyme embedded cryogel for CO2 conversion
Authors: Biçen Ünlüer Özlem , Sümbelli Yiğitcan , Hür Deniz , Say Rıdvan , Ersöz Arzu 
Published: October 2023

AbstractCO2 conversion to formic acid in a single‐step reaction was investigated by incorporating MoSe2 nanoparticles into a cryogel‐based reactor system, resulting in a formate dehydrogenase (FDH) enzyme‐mimicking system. MoSe2 nanoparticles were synthesized via the microwave synthesis method, and they took part as the cofactor of the enzymatic system. Following, in combination with MACys‐Ru(bipyr)2‐MACys, MoSe2 nanoparticles were used as the cofactor of the conversion reaction in the MACys‐Ru(bipyr)2‐MACys‐co‐p(HEMA) cryogel column‐based reactor system. Synthesized nanoparticles were characterized by Fourier transformed infrared spectroscopy, field emission‐scanning electron microscopy, transmission electron microscopy, and energy dispersive x‐ray spectroscopy. The conversion reaction was monitored using high‐pressure liquid chromatography, and the parameters affecting the conversion yield (e.g., pH change and reusability of the reactor) were investigated. The results showed that the obtained mimic FDH‐based CO2 conversion column system worked like a reactor in the conversion of CO2 into formic acid. Besides, the developed cryogenic reactor provided a reusable single‐step conversion reaction with a high yield at room temperature in mild conditions.
Interaction between huntingtin exon 1 and HEAT repeat structure probed by chimeric model proteins
Authors: Zhang Hong , Wu Si , Itzhaki Laura S. , Perrett Sarah 
Published: October 2023

AbstractHuntington disease (HD) is associated with aggregation of huntingtin (HTT) protein containing over 35 continuous Q residues within the N‐terminal exon 1 encoded region. The C‐terminal of the HTT protein consists mainly of HEAT repeat structure which serves as a scaffold for multiple cellular activities. Structural and biochemical analysis of the intact HTT protein has been hampered by its huge size (~300 kDa) and most in vitro studies to date have focused on the properties of the exon 1 region. To explore the interaction between HTT exon 1 and the HEAT repeat structure, we constructed chimeric proteins containing the N‐terminal HTT exon 1 region and the HEAT repeat protein PR65/A. The results indicate that HTT exon 1 slightly destabilizes the downstream HEAT repeat structure and endows the HEAT repeat structure with more conformational flexibility. Wild‐type and pathological lengths of polyQ did not show differences in the interaction between HTT exon 1 and the HEAT repeats. With the C‐terminal fusion of PR65/A, HTT exon 1 containing pathological lengths of polyQ could still form amyloid fibrils, but the higher‐order architecture of fibrils and kinetics of fibril formation were affected by the C‐terminal fusion of HEAT repeats. This indicates that interaction between HTT exon 1 and HEAT repeat structure is compatible with both normal function of HTT protein and the pathogenesis of HD, and this study provides a potential model for further exploration.