bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal immune activation during pregnancy is associated with more difficulties in socio-adaptive behaviors in autism spectrum disorder
Authors: Ellul Pierre , Maruani Anna , Vantalon Valérie , Humeau Elise , Amestoy Anouck , Anchordoqui Andrea , Atzori Paola , Baleyte Jean-Marc , Benmansour Safiyah , Bonnot Olivier , Bouvard Manuel , Cartigny Ariane , Coulon Nathalie , Coutelle Romain , Da Fonseca David , Demily Caroline , Givaudan Marion , Gollier-Briant Fanny , Guénolé Fabian , Koch Andrea , Leboyer Marion , Lefebvre Aline , Lejuste Florian , Levy Charlotte , Mendes Eugénie , Robert Natalia , Schroder Carmen M. , Speranza Mario , Zante Elodie , Peyre Hugo , Rosenzwajg Michelle , Klatzmann David , Tchitchek Nicolas , Delorme Richard 
Published: October 2023

AbstractAutism spectrum disorder (ASD) are neurodevelopmental conditions characterised by deficits in social communication and interaction and repetitive behaviours. Maternal immune activation (MIA) during the mid-pregnancy is a known risk factor for ASD. Although reported in 15% of affected individuals, little is known about the specificity of their clinical profiles. Adaptive skills represent a holistic approach to a person's competencies and reflect specifically in ASD, their strengths and difficulties. In this study, we hypothesised that ASD individual with a history of MIA (MIA+) could be more severely socio-adaptively impaired than those without MIA during pregnancy (MIA-). To answer this question, we considered two independent cohorts of individuals with ASD (PARIS study and FACE ASD) screened for pregnancy history, and used supervised and unsupervised machine learning algorithms. We included 295 mother–child dyads with 14% of them with MIA+. We found that ASD-MIA+ individuals displayed more severe maladaptive behaviors, specifically in their socialization abilities. MIA+ directly influenced individual's socio-adaptive skills, independent of other covariates, including ASD severity. Interestingly, MIA+ affect persistently the socio-adaptive behavioral trajectories of individuals with ASD. The current study has a retrospective design with possible recall bias regarding the MIA event and, even if pooled from two cohorts, has a relatively small population. In addition, we were limited by the number of covariables available potentially impacted socio-adaptive behaviors. Larger prospective study with additional dimensions related to ASD is needed to confirm our results. Specific pathophysiological pathways may explain these clinical peculiarities of ASD- MIA+ individuals, and may open the way to new perspectives in deciphering the phenotypic complexity of ASD and for the development of specific immunomodulatory strategies.
Fine conductive line printing of high viscosity CuO ink using near field electrospinning (NFES)
Authors: Rahman Md. Khalilur , Lee Jin-Sol , Kwon Kye-Si 
Published: October 2023

AbstractModern printed electronics applications require patterning of fine conductive lines of sufficient thickness. However, the two requirements for pattern width and thickness are a trade-off. To print fine pattern at a micrometer size, the nozzle diameter must be approximately the size of the pattern width, so only low-viscosity inks are used. As a result, the pattern is likely to be very thin and multiple overlapping printing is required for sufficient conductance. In order to use high viscosity ink for fine patterning, near field electrospinning (NFES) is attracting attention because it can print very thin and thick patterns using large nozzles (high-viscosity ink). Until now, silver paste ink has been used for microconductive patterning using electrospinning. However, Ag nanoparticle (NP) inks are expensive. In this study, we report the use of a relatively inexpensive CuO NP ink for electrospinning-based printing. For implementation, the material preparation, printing and post-processing process are discussed. For post-processing, a continuous wave (CW) green laser with a 532 nm wavelength was used to reduce the CuO to Cu and sinter the nanoparticles. After sintering, the 50 μm width and 1.48 μm thick Cu conductive line exhibited a resistivity of 5.46 μΩ·cm, which is 3.25 times of the bulk resistivity of Cu.
Application of single wrist-wearable accelerometry for objective motor diary assessment in fluctuating Parkinson’s disease
Authors: Löhle Matthias , Timpka Jonathan , Bremer Alexander , Khodakarami Hamid , Gandor Florin , Horne Malcom , Ebersbach Georg , Odin Per , Storch Alexander 
Published: October 2023

AbstractAdvanced Parkinson’s disease (PD) is characterized by motor fluctuations including unpredictable oscillations remarkably impairing quality of life. Effective management and development of novel therapies for these response fluctuations largely depend on clinical rating instruments such as the widely-used PD home diary, which are associated with biases and errors. Recent advancements in digital health technologies provide user-friendly wearables that can be tailored for continuous monitoring of motor fluctuations. Their criterion validity under real-world conditions using clinical examination as the gold standard remains to be determined. We prospectively examined this validity of a wearable accelerometer-based digital Parkinson’s Motor Diary (adPMD) using the Parkinson’s Kinetigraph (PKG®) in an alternative application by converting its continuous data into one of the three motor categories of the PD home diary (Off, On and Dyskinetic state). Sixty-three out of 91 eligible participants with fluctuating PD (46% men, average age 66) had predefined sufficient adPMD datasets (>70% of half-hour periods) from 2 consecutive days. 92% of per-protocol assessments were completed. adPMD monitoring of daily times in motor states showed moderate validity for Off and Dyskinetic state (ICC = 0.43–0.51), while inter-rating methods agreements on half-hour-level can be characterized as poor (median Cohen’s κ = 0.13–0.21). Individualization of adPMD thresholds for transferring accelerometer data into diary categories improved temporal agreements up to moderate level for Dyskinetic state detection (median Cohen’s κ = 0.25–0.41). Here we report that adPMD real-world-monitoring captures daily times in Off and Dyskinetic state in advanced PD with moderate validities, while temporal agreement of adPMD and clinical observer diary data is limited.
The neonatal southern white rhinoceros ovary contains oogonia in germ cell nests
Authors: Appeltant Ruth , Hermes Robert , Holtze Susanne , Modina Silvia Clotilde , Galli Cesare , Bjarkadottir Briet. D. , Adeniran Babatomisin V. , Wei Xi , Swegen Aleona , Hildebrandt Thomas Bernd , Williams Suzannah A. 
Published: October 2023

AbstractThe northern white rhinoceros is functionally extinct with only two females left. Establishing methods to culture ovarian tissues, follicles, and oocytes to generate eggs will support conservation efforts using in vitro embryo production. To the best of our knowledge, this is the first description of the structure and molecular signature of any rhinoceros, more specifically, we describe the neonatal and adult southern white rhinoceros (Ceratotherium simum simum) ovary; the closest relation of the northern white rhinoceros. Interestingly, all ovaries contain follicles despite advanced age. Analysis of the neonate reveals a population of cells molecularly characterised as mitotically active, pluripotent with germ cell properties. These results indicate that unusually, the neonatal ovary still contains oogonia in germ cell nests at birth, providing an opportunity for fertility preservation. Therefore, utilising ovaries from stillborn and adult rhinoceros can provide cells for advanced assisted reproductive technologies and investigating the neonatal ovaries of other endangered species is crucial for conservation.
Scanning single molecule localization microscopy (scanSMLM) for super-resolution volume imaging
Authors: Basumatary Jigmi , Baro Neptune , Joshi Prakash , Mondal Partha Pratim 
Published: October 2023

AbstractOver the last decade, single-molecule localization microscopy (SMLM) has developed into a set of powerful techniques that have improved spatial resolution over diffraction-limited microscopy and demonstrated the ability to resolve biological features down to a few tens of nanometers. We introduce a single molecule-based scanning SMLM (scanSMLM) system that enables rapid volume imaging. Along with epi-illumination, the system employs a scanning-based 4f detection for volume imaging. The 4f system comprises a combination of an electrically-tunable lens and high NA detection objective lens. By rapidly changing the aperture (or equivalently the focus) of an electrically-tunable lens (ETL) in a 4f detection system, the selectivity of the axial object plane is achieved, for which the image forms in the image/detector plane. So, in principle, one can scan the object volume by just altering the aperture of ETL. Two schemes were adopted to carry out volume imaging: cyclic scan and conventional scan. The cyclic scheme scans the volume in each scan cycle, whereas plane-wise scanning is performed in the conventional scheme. Hence, the cyclic scan ensures uniform dwell time on each frame during data collection, thereby evenly distributing photobleaching throughout the cell volume. With a minimal change in the system hardware (requiring the addition of an ETL lens and related electronics for step-voltage generation) in the existing SMLM system, volume scanning (along the z-axis) can be achieved. To calibrate and derive critical system parameters, we imaged fluorescent beads embedded in a gel-matrix 3D block as a test sample. Subsequently, scanSMLM is employed to visualize the architecture of actin-filaments and the distribution of Meos-Tom20 molecules on the mitochondrial membrane. The technique is further exploited to understand the clustering of Hemagglutinin (HA) protein single molecules in a transfected cell for studying Influenza-A disease progression. The system, for the first time, enabled 3D visualization of HA distribution that revealed HA cluster formation spanning the entire cell volume, post 24 hrs of transfection. Critical biophysical parameters related to HA clusters (density, the number of HA molecules per cluster, axial span, fraction of clustered molecules, and others) are also determined, giving an unprecedented insight into Influenza-A disease progression at the single-molecule level.
Single-cell chromatin accessibility and transcriptomic characterization of Behcet’s disease
Authors: Shi Wen , Ye Jinguo , Shi Zhuoxing , Pan Caineng , Zhang Qikai , Lin Yuheng , Liang Dan , Liu Yizhi , Lin Xianchai , Zheng Yingfeng 
Published: October 2023

AbstractBehect’s disease is a chronic vasculitis characterized by complex multi-organ immune aberrations. However, a comprehensive understanding of the gene-regulatory profile of peripheral autoimmunity and the diverse immune responses across distinct cell types in Behcet’s disease (BD) is still lacking. Here, we present a multi-omic single-cell study of 424,817 cells in BD patients and non-BD individuals. This study maps chromatin accessibility and gene expression in the same biological samples, unraveling vast cellular heterogeneity. We identify widespread cell-type-specific, disease-associated active and pro-inflammatory immunity in both transcript and epigenomic aspects. Notably, integrative multi-omic analysis reveals putative TF regulators that might contribute to chromatin accessibility and gene expression in BD. Moreover, we predicted gene-regulatory networks within nominated TF activators, including AP-1, NF-kB, and ETS transcript factor families, which may regulate cellular interaction and govern inflammation. Our study illustrates the epigenetic and transcriptional landscape in BD peripheral blood and expands understanding of potential epigenomic immunopathology in this disease.
Determinants of species-specific utilization of ACE2 by human and animal coronaviruses
Authors: Wang Qingxing , Noettger Sabrina , Xie Qinya , Pastorio Chiara , Seidel Alina , Müller Janis A. , Jung Christoph , Jacob Timo , Sparrer Konstantin M. J. , Zech Fabian , Kirchhoff Frank 
Published: October 2023

AbstractUtilization of human ACE2 allowed several bat coronaviruses (CoVs), including the causative agent of COVID-19, to infect humans directly or via intermediate hosts. However, the determinants of species-specific differences in ACE2 usage and the frequency of the ability of animal CoVs to use human ACE2 are poorly understood. Here we applied VSV pseudoviruses to analyze the ability of Spike proteins from 26 human or animal CoVs to use ACE2 receptors across nine reservoir, potential intermediate and human hosts. We show that SARS-CoV-2 Omicron variants evolved towards more efficient ACE2 usage but mutation of R493Q in BA.4/5 and XBB Spike proteins disrupts utilization of ACE2 from Greater horseshoe bats. Variations in ACE2 residues 31, 41 and 354 govern species-specific differences in usage by coronaviral Spike proteins. Mutation of T403R allows the RaTG13 bat CoV Spike to efficiently use all ACE2 orthologs for viral entry. Sera from COVID-19 vaccinated individuals neutralize the Spike proteins of various bat Sarbecoviruses. Our results define determinants of ACE2 receptor usage of diverse CoVs and suggest that COVID-19 vaccination may protect against future zoonoses of bat coronaviruses.
Platicon dynamics in photonic molecules
Authors: Rebolledo-Salgado Israel , Quevedo-Galán Clara , Helgason Óskar Bjarki , Lööf Anton , Ye Zhichao , Lei Fuchuan , Schröder Jochen , Zelan Martin , Torres-Company Victor 
Published: October 2023

AbstractCoherent dissipative structures known as platicons can be reliably generated in photonic molecules, resulting in deterministic and reproducible microcombs derived from a continuous-wave pump. However, the supermode spectrum of standard photonic molecules displays numerous avoided mode crossings, distorting the spectral envelope of platicon microcombs. Here, we obtain a platicon microcomb using a photonic molecule configuration based on two coupled microcavities, whose size differs by an order of magnitude. This results in an engineered microcomb spectrum that closely resembles the one generated in an ideal single microresonator with just one frequency mode shift. We observe the coupling between the repetition rate of the platicon microcomb with the frequency of the pump laser, an effect originating from the dispersive-wave recoil induced by mode crossings. Using two identical platicon microcombs, we make use of such coupling to realize dual-comb interferometry. These results contribute to understanding dissipative structures in normal-dispersion microresonators and offer an alternative to applications such as spectroscopy and metrology.
The overall-subshear and multi-segment rupture of the 2023 Mw7.8 Kahramanmaraş, Turkey earthquake in millennia supercycle
Authors: Xu Liuwei , Mohanna Saeed , Meng Lingsen , Ji Chen , Ampuero Jean-Paul , Yunjun Zhang , Hasnain Masooma , Chu Risheng , Liang Cunren 
Published: October 2023

AbstractOn February 6, 2023, an Mw7.8 earthquake hit the East Anatolian Fault (EAF) and Narlı Fault (NF), followed by an Mw7.5 event on the Sürgü Fault. We combine multiple seismic datasets, global navigation satellite system recordings, and radar satellite images with finite fault inversion and slowness enhanced back-projection to study the rupture kinematics. Our analysis reveals that the rupture originated on the NF, propagating 120 km northeast at 3.05 km/s and 200 km southwest at 3.11 km/s after reaching the EAF junction, exhibiting overall subshear speeds. Further Mach wave analysis confirms the subshear rupture, matching the prediction using close-Rayleigh speeds. The unexpectedly-large slip on some EAF segments suggests a supercycle lasting ≥900 years. The EAF geometry is similar to the San Andreas-San Jacinto Fault system, while the latter has higher slip rates but without large earthquakes on its southern segments since 1857, carrying the potential of an M8 earthquake.
Microbial growth in actual martian regolith in the form of Mars meteorite EETA79001
Authors: Naz Neveda , Harandi Bijan F. , Newmark Jacob , Kounaves Samuel P. 
Published: October 2023

AbstractStudies to understand the growth of organisms on Mars are hampered by the use of simulants to duplicate martian mineralogy and chemistry. Even though such materials are improving, no terrestrial simulant can replace a real martian sample. Here we report the use of actual martian regolith, in the form of Mars meteorite EETA79001 sawdust, to demonstrate its ability to support the growth of four microorganisms,E. coli. Eucapsissp.,Chr20-20201027-1, andP. halocryophilus, for up to 23 days under terrestrial conditions using regolith:water ratios from 4:1 to 1:10. If the EETA79001 sawdust is widely representative of regolith on the martian surface, our results imply that microbial life under appropriate conditions could have been present on Mars in the past and/or today in the subsurface, and that the regolith does not contain any bactericidal agents. The results of our study have implications not only for putative martian microbial life but also for building bio-sustainable human habitats on Mars.
Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome
Authors: Bertagnolli Anthony D , Maritan Andrew J , Tumolo Benjamin B , Fritz Samuel F , Oakland Hayley C , Mohr Elizabeth J , Poole Geoffrey C , Albertson Lindsey K , Stewart Frank J 
Published: October 2023

Abstract
Larval net-spinning caddisflies (Hydropsychidae) function as ecosystem engineers in streams where they construct protective retreats composed of organic and inorganic material affixed with silk filtration nets that alter streambed hydrology. We hypothesized that hydropsychid bio-structures (retreats, nets) are microhabitats for microbes with oxygen-sensitive metabolisms, and therefore increase the metabolic heterogeneity of streambed microbial assemblages. Metagenomic and 16 S rRNA gene amplicon analysis of samples from a montane stream (Cherry Creek, Montana, USA) revealed that microbiomes of caddisfly bio-structures are taxonomically and functionally distinct from those of the immediately adjacent rock biofilm (~2 cm distant) and enriched in microbial taxa with established roles in denitrification, nitrification, and methane production. Genes for denitrification, high oxygen affinity terminal oxidases, hydrogenases, oxidative dissimilatory sulfite reductases, and complete ammonia oxidation are significantly enriched in caddisfly bio-structures. The results suggest a novel ecosystem engineering effect of caddisflies through the creation of low-oxygen, denitrifier-enriched niches in the stream microbiome. Facilitation of metabolic diversity in streambeds may be a largely unrecognized mechanism by which caddisflies alter whole-stream biogeochemistry.
Extracellular vesicles are the main contributor to the non-viral protected extracellular sequence space
Authors: Lücking Dominik , Mercier Coraline , Alarcón-Schumacher Tomas , Erdmann Susanne 
Published: October 2023

Abstract
Environmental virus metagenomes, commonly referred to as “viromes”, are typically generated by physically separating virus-like particles (VLPs) from the microbial fraction based on their size and mass. However, most methods used to purify VLPs, enrich extracellular vesicles (EVs) and gene transfer agents (GTAs) simultaneously. Consequently, the sequence space traditionally referred to as a “virome” contains host-associated sequences, transported via EVs or GTAs. We therefore propose to call the genetic material isolated from size-fractionated (0.22 µm) and DNase-treated samples protected environmental DNA (peDNA). This sequence space contains viral genomes, DNA transduced by viruses and DNA transported in EVs and GTAs. Since there is no genetic signature for peDNA transported in EVs, GTAs and virus particles, we rely on the successful removal of contaminating remaining cellular and free DNA when analyzing peDNA. Using marine samples collected from the North Sea, we generated a thoroughly purified peDNA dataset and developed a bioinformatic pipeline to determine the potential origin of the purified DNA. This pipeline was applied to our dataset as well as existing global marine “viromes”. Through this pipeline, we identified known GTA and EV producers, as well as organisms with actively transducing proviruses as the source of the peDNA, thus confirming the reliability of our approach. Additionally, we identified novel and widespread EV producers, and found quantitative evidence suggesting that EV-mediated gene transfer plays a significant role in driving horizontal gene transfer (HGT) in the world’s oceans.
Absolute thermometry of human brown adipose tissue by magnetic resonance with laser polarized 129Xe
Authors: Zhang Le , Antonacci Michael , Burant Alex , McCallister Andrew , Kelley Michele , Bryden Nicholas , McHugh Christian , Atalla Sebastian , Holmes Leah , Katz Laurence , Branca Rosa Tamara 
Published: October 2023

Abstract
Background
Absolute temperature measurements of tissues inside the human body are difficult to perform non-invasively. Yet, for brown adipose tissue (BAT), these measurements would enable direct monitoring of its thermogenic activity and its association with metabolic health.

Methods
Here, we report direct measurement of absolute BAT temperature in humans during cold exposure by magnetic resonance (MR) with laser polarized xenon gas. This methodology, which leverages on the sensitivity of the chemical shift of the 129Xe isotope to temperature-induced changes in fat density, is first calibrated in vitro and then tested in vivo in rodents. Finally, it is used in humans along with positron emission tomography (PET) scans with fluorine-18-fluorodeoxyglucose to detect BAT thermogenic activity during cold exposure.

Results
Absolute temperature measurements, obtained in rodents with an experimental error of 0.5 °C, show only a median deviation of 0.12 °C against temperature measurements made using a pre-calibrated optical temperature probe. In humans, enhanced uptake of 129Xe in BAT during cold exposure leads to background-free detection of this tissue by MR. Global measurements of supraclavicular BAT temperature, made over the course of four seconds and with an experimental error ranging from a minimum of 0.4 °C to more than 2 °C, in case of poor shimming, reveal an average BAT temperature of 38.8° ± 0.8 °C, significantly higher (p < 0.02 two-sided t test) than 37.7 °C. Hot BAT is also detected in participants with a PET scan negative for BAT.

Conclusions
Non-invasive, radiation-free measurements of BAT temperature by MRI with hyperpolarized 129Xe may enable longitudinal monitoring of human BAT activity under various stimulatory conditions.
Ätherische Öle als Hemmer des bakteriellen Quorum
Sensings
Authors: Melzig Matthias F. 
Published: October 2023

ZusammenfassungAntibiotikaresistente Krankheitserreger stellen eine zunehmende Gefahr bei der
Behandlung von Infektionen dar. Insbesondere Biofilme als
Bakteriengemeinschaften rücken hier in den Fokus, da gegen sie
klassische Antibiotika zumeist wirkungslos sind. Eine wichtige Rolle bei der
Biofilmbildung spielt das Quorum Sensing, das durch ätherische
Öle gestört werden kann.