bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patterns of publicly funded naltrexone use among patients diagnosed with alcohol use disorder in Ontario
Authors: Tourchian Nima , McCormack Daniel , Leece Pamela , Tadrous Mina , Gomes Tara 
Published: February 2024

Abstract

Aims
Naltrexone is recommended first-line to manage alcohol use disorder (AUD). With previous studies indicating poor retention on naltrexone, we determined duration of naltrexone use and assessed the association between prescription setting and time to discontinuation in Ontario.

Methods
We conducted a retrospective population-based cohort study among Ontario public drug beneficiaries diagnosed with AUD who initiated publicly funded naltrexone from June 2018 to September 2019. The primary outcome was time to naltrexone discontinuation, with a secondary analysis assessing receipt of at least one prescription refill. We used Cox proportional hazards models and logistic regression to test the association between prescription setting and each medication persistence outcome.

Results
Among 2531 new naltrexone patients with AUD, the median duration of naltrexone use was 31 days and 394 (15.6%) continued naltrexone for 6 months or longer. There was no association between setting of initiation and duration of naltrexone use; however, those initiating naltrexone following an acute inpatient hospital stay were more likely to fill a second prescription (aOR 1.43, 95% CI 0.96–2.14), while those initiating after an ED visit were less likely to be dispensed a second prescription (aOR = 0.69, 95% CI 0.52–0.90) compared to those starting in a physician’s office.

Conclusion
Persistence on naltrexone to treat an AUD is low, regardless of the setting of initiation. Further research is needed to elucidate the barriers encountered by patients with AUD that lead to poor treatment persistence in order to develop interventions that facilitate patient-centered access to evidence-based treatment for AUD in the province.
Sensory trap leads to reliable communication without a shift in nonsexual responses to the model cue
Authors: Fissette Skye D , Buchinger Tyler J , Tamrakar Sonam , Scott Anne M , Li Weiming 
Published: February 2024

Abstract
The sensory trap model of signal evolution suggests that males manipulate females into mating using traits that mimic cues used in a nonsexual context. Despite much empirical support for sensory traps, little is known about how females evolve in response to these deceptive signals. Female sea lamprey (Petromyzon marinus) evolved to discriminate a male sex pheromone from the larval odor it mimics and orient only toward males during mate search. Larvae and males release the attractant 3-keto petromyzonol sulfate (3kPZS), but spawning females avoid larval odor using the pheromone antagonist, petromyzonol sulfate (PZS), which larvae but not males, release at higher rates than 3kPZS. We tested the hypothesis that migratory females also discriminate between larval odor and the male pheromone and orient only to larval odor during anadromous migration, when they navigate within spawning streams using larval odor before they begin mate search. In-stream behavioral assays revealed that, unlike spawning females, migratory females do not discriminate between mixtures of 3kPZS and PZS applied at ratios typical of larval versus male odorants. Our results indicate females discriminate between the sexual and nonsexual sources of 3kPZS during but not outside of mating and show sensory traps can lead to reliable sexual communication without females shifting their responses in the original context.
scMMT: a multi-use deep learning approach for cell annotation, protein prediction and embedding in single-cell RNA-seq data
Authors: Zhou Songqi , Li Yang , Wu Wenyuan , Li Li 
Published: February 2024

Abstract
Accurate cell type annotation in single-cell RNA-sequencing data is essential for advancing biological and medical research, particularly in understanding disease progression and tumor microenvironments. However, existing methods are constrained by single feature extraction approaches, lack of adaptability to immune cell types with similar molecular profiles but distinct functions and a failure to account for the impact of cell label noise on model accuracy, all of which compromise the precision of annotation. To address these challenges, we developed a supervised approach called scMMT. We proposed a novel feature extraction technique to uncover more valuable information. Additionally, we constructed a multi-task learning framework based on the GradNorm method to enhance the recognition of challenging immune cells and reduce the impact of label noise by facilitating mutual reinforcement between cell type annotation and protein prediction tasks. Furthermore, we introduced logarithmic weighting and label smoothing mechanisms to enhance the recognition ability of rare cell types and prevent model overconfidence. Through comprehensive evaluations on multiple public datasets, scMMT has demonstrated state-of-the-art performance in various aspects including cell type annotation, rare cell identification, dropout and label noise resistance, protein expression prediction and low-dimensional embedding representation.
Elongation of intercostal nerve cutaneous branches for breast and nipple neurotization during breast reconstruction after mastectomy for breast cancer: case–control study
Authors: Chang Tommy Nai-Jen , Lu Johnny Chuieng-Yi , Sung Cheyenne Wei-Hsuan , Illias Amina M , Cheong David Chon-Fok , Kao Shu-Wei , Kuo Wen-Ling , Pindur Lukas , Chen Lisa Wen-Yu , Lin Jennifer An-Jou , Chuang David Chwei-Chin , Huang Jung-Ju 
Published: February 2024

Abstract

Background
To restore sensation after breast reconstruction, a modified surgical approach was employed by identifying the cut fourth intercostal lateral cutaneous branch, elongating it with intercostal nerve grafts, and coapting it to the innervating nerve of the flap or by using direct neurotization of the spared nipple/skin.

Methods
This was a retrospective case–control study including 56 patients who underwent breast neurotization surgery. Breast operations included immediate reconstruction after nipple-sparing mastectomy (36 patients), skin-sparing mastectomy (8 patients), and delayed reconstruction with nipple preservation (7 patients) or without nipple preservation (5 patients). Patients who underwent breast reconstruction without neurotization were included as the non-neurotization negative control group. The contralateral normal breasts were included as positive controls.

Results
The mean(s.d.) monofilament test values were 0.07(0.10) g for the positive control breasts and 179.13(143.31) g for the breasts operated on in the non-neurotization group. Breasts that underwent neurotization had significantly better sensation after surgery, with a mean(s.d.) value of 35.61(92.63) g (P &lt; 0.001). The mean(s.d.) sensory return after neurotization was gradual; 138.17(143.65) g in the first 6 months, 59.55(116.46) g at 7–12 months, 14.54(62.27) g at 13–18 months, and 0.37(0.50) g at 19–24 months after surgery. Two patients had accidental rupture of the pleura, which was repaired uneventfully. One patient underwent re-exploration due to a lack of improvement 1.5 years after neurotization.

Conclusion
Using the lateral cutaneous branch of the intercostal nerve as the innervating stump and elongating it with intercostal nerve grafts is a suitable technique to restore sensation after mastectomy. This method effectively innervates reconstructed breasts and spares the nipple/skin with minimal morbidity.
EEG evidence for racial ingroup bias in collective empathy for pain
Authors: Mei Shuting , Weiß Martin , Hein Grit , Han Shihui 
Published: February 2024

Abstract
Previous research on racial ingroup bias in empathy for pain focused on neural responses to a single person’s suffering. It is unclear whether empathy for simultaneously perceived multiple individuals’ pain (denoted as collective empathy in this study) is also sensitive to perceived racial identities of empathy targets. We addressed this issue by recording electroencephalography from Chinese adults who responded to racial identities of 2 × 2 arrays of Asian or White faces in which 4 faces, 1 face, or no face showed painful expressions. Participants reported greater feelings of others’ pain and their own unpleasantness when viewing 4 compared to 1 (or no) painful faces. Behavioral responses to racial identities of faces revealed decreased speeds of information acquisition when responding to the face arrays with 4 (vs. 1 or no) painful expressions of Asian (but not White) faces. Moreover, Asian compared to White face arrays with 4 (vs. 1 or no) painful expressions elicited a larger positive neural response at 160–190 ms (P2) at the frontal/central electrodes and enhanced alpha synchronizations at 288–1,000 ms at the central electrodes. Our findings provide evidence for racial ingroup biases in collective empathy for pain and unravel its relevant neural underpinnings.
Circulating Cell-Free SHOX2 DNA Methylation Is a Predictive, Prognostic, and Monitoring Biomarker in Adjuvant and Palliative Anti-PD-1-Treated Melanoma
Authors: Fietz Simon , Diekmann Eric , de Vos Luka , Zarbl Romina , Hunecke Alina , Glosch Ann-Kathrin , Färber Moritz , Sirokay Judith , Hoffmann Friederike , Fröhlich Anne , Franzen Alina , Strieth Sebastian , Landsberg Jennifer , Dietrich Dimo 
Published: February 2024

Abstract

Background
The majority of metastatic melanoma patients initially do not respond or acquire resistance to anti-programmed cell death 1 (PD-1) immunotherapy. Liquid biopsy biomarkers might provide useful early response information and allow for personalized treatment decisions.

Methods
We prospectively assessed circulating cell-free SHOX2 DNA methylation (SHOX2 ccfDNAm) levels and their dynamic changes in blood plasma of melanoma patients by quantitative methylation-specific polymerase chain reaction. Patients were treated with either palliative (n = 42) or adjuvant (n = 55) anti-PD-1 immunotherapy. Moreover, we included n = 126 control patients without evidence of malignant disease. We analyzed SHOX2 ccfDNAm status prior to and 4 weeks after palliative treatment initiation with regard to outcome [objective response, progression-free survival (PFS), and overall survival (OS)]. In the adjuvant setting, we associated longitudinal SHOX2 ccfDNAm status with disease recurrence.

Results
Sensitivity was 60% with 25/42 melanoma patients showing increased SHOX2 ccfDNAm levels, whereas specificity was 98% with 123/126 (P &lt; 0.001) control patients having SHOX2 ccfDNAm levels below cut-off. Pretreatment SHOX2 ccfDNAm status did not correlate with outcome; however, SHOX2 ccfDNAm negativity 4 weeks after palliative treatment initiation was strongly associated with improved survival [PFS: hazard ratio (HR) = 0.25, P = 0.002; OS: HR = 0.12, P = 0.007]. Pretreatment positive patients who reached SHOX2 ccfDNAm clearance after 4 weeks of immunotherapy showed an exceptionally beneficial outcome. SHOX2 ccfDNAm testing allowed for an early detection of distant metastases in adjuvant-treated melanoma patients.

Conclusions
Our study suggests SHOX2 ccfDNAm to be an early predictor of outcome in anti-PD-1 treated melanoma patients. SHOX2 ccfDNAm testing may aid individualized treatment decision-making.
NanoLAS: a comprehensive nanobody database with data integration, consolidation and application
Authors: Xiong Shuchang , Liu Zhengwen , Yi Xin , Liu Kai , Huang Bingding , Wang Xin 
Published: February 2024

Abstract
Nanobodies, a unique subclass of antibodies first discovered in camelid animals, are composed solely of a single heavy chain’s variable region. Their significantly reduced molecular weight, in comparison to conventional antibodies, confers numerous advantages in the treatment of various diseases. As research and applications involving nanobodies expand, the quantity of identified nanobodies is also rapidly growing. However, the existing antibody databases are deficient in type and coverage, failing to satisfy the comprehensive needs of researchers and thus impeding progress in nanobody research.
In response to this, we have amalgamated data from multiple sources to successfully assemble a new and comprehensive nanobody database. This database has currently included the latest nanobody data and provides researchers with an excellent search and data display interface, thus facilitating the progression of nanobody research and their application in disease treatment.
In summary, the newly constructed Nanobody Library and Archive System may significantly enhance the retrieval efficiency and application potential of nanobodies. We envision that Nanobody Library and Archive System will serve as an accessible, robust and efficient tool for nanobody research and development, propelling advancements in the field of biomedicine.
Database URL: https://www.nanolas.cloud
Dependent observations in clinical research
Authors: Groenwold Rolf H H , Dekkers Olaf M 
Published: February 2024

Abstract
For many statistical methods that are commonly used in medical research, it is assumed that observations are independent. However, when this assumption is violated, alternative methods may be needed. In this paper, we describe why an analysis that ignores the dependencies within the data may provide biased results and outline possible alternatives. This paper aims to create awareness among researchers, readers, and reviewers for this possible source of bias in medical research.
Overview of the participation of community health workers in primary health care in 6 Latin American countries and a proposal for their integration into the health system: a qualitative study
Authors: Zuñiga Rosalia Olaya , Parra-García Irene , Gómez-Barrera Luis Alejandro 
Published: February 2024

Abstract

Background
According to some health programmes, implementing primary health care through community health workers (CHWs) facilitates the connection between community and health services in Latin America. However, these are isolated processes that face different obstacles and would benefit from an overview of the corresponding health policies and programmes.

Objective
To provide an overview of CHW participation in 6 Latin American countries.

Methods
This exploratory qualitative study was based on 3 sources of information: a literature review, a review of public health policy documents, and interviews with experts who have led CHW programmes in 6 Latin American countries.

Results
The role of CHWs in Latin America and some advances in public health policies in the region were evidenced. However, limitations arising from variable implementation of the WHO guidelines on health programmes with CHWs were also apparent.

Conclusions
CHWs contribute to the primary healthcare processes in the 6 Latin American countries studied in versatile and comprehensive ways. However, they constitute an underutilized human resource because they must provide various services that are not always relevant in different work contexts. Therefore, we propose a classification of the CHW profile, using the level of access to healthcare services of the population they serve as the main differentiator. This way, CHWs will not have to provide a wide range of services but only those most relevant to the specific needs of each community.
Appraisal of National Institute for Health and Care Research activity in primary care in England: cross-sectional study
Authors: Jonker Leon , Jayne Fisher Stacey 
Published: February 2024

Abstract

Background
The National Institute for Health and Care Research (NIHR) was set up to enhance clinical and health research activity in a variety of National Health Service (NHS) healthcare settings, including primary care.

Objective
To appraise how overall General Practitioner (GP) practice performance, location, and staffing levels may interact with NIHR Portfolio activity in primary care in England.

Methods
Cross-sectional summary of GP practice research activity and practice descriptors; complete data from 6,171 GP practices was collated from NIHR (using data for 2013–2023 for Portfolio studies), Public Health England, Care Quality Commission, and NHS Digital sources, respectively.

Results
In primary care, 1 million patients have been recruited into NIHR Portfolio studies in the last decade. The top 10% of practices—measured by different studies recruited to—contributed over 50% of that accrual. When the top decile of GP practices is compared to the 20% least active GP practices, research activity is significantly and individually linked with larger GP practices. Furthermore, it is significantly yet modestly associated with GP practice performance (positive patient feedback, Care Quality Commission rating), lower locality deprivation levels, and lower patient to GP ratios.

Conclusions
Research activity in GP practices is—as seen previously with hospitals—significantly linked with better GP practice performance and patient feedback. Practice list size and staffing levels in particular interact with the aforementioned. This should be taken into account when determining strategies to increase patient and GP practice participation in NIHR Portfolio research studies.
Insights Into Informal Caregivers’ Well-being: A Longitudinal Analysis of Care Intensity, Care Location, and Care Relationship
Authors: Zhang Yanan , Bennett Matthew R 
Published: February 2024

Abstract

Objectives
This study investigates the psychological well-being of informal caregivers over time. It identifies the thresholds (or “tipping points”) of caring intensity at which caregiving is associated with lower psychological well-being, and how this varies by care location and caregiver–care recipient relationships. It also examines how caring location and relationship are linked to informal caregivers’ psychological well-being while controlling for caring intensity.

Methods
Waves 1–18 (1991–2009) of the harmonized British Household Panel Survey and Waves 1–8 (2009–2017) of the U.K. Household Longitudinal Study were analyzed. Psychological well-being was measured using the General Health Questionnaire (GHQ)-12 score. Care intensity was measured by the weekly hours of care provided. Fixed-effects estimators were applied to the GHQ-12 score of caregivers across different care intensities, caring locations, and caring relationships.

Results
All levels of informal care intensity are associated with lower psychological well-being among spousal caregivers. The thresholds to well-being are 5 hours per week when caring for a parent, and 50 hours per week when caring for a child (with a disability or long-term illness). Caring for “other relatives” or nonrelatives is not negatively associated with psychological well-being. The thresholds are 5 hours per week for both coresident and extraresident caregivers. Extraresident caregivers experience better psychological well-being compared to coresident caregivers, given relatively lower weekly care hours. Caring for primary kin (especially spouses) is linked to lower psychological well-being compared to other caregiving relationships, regardless of care intensity.

Discussion
Policy and practice responses should pay particular attention to spousal caregivers’ well-being. Caregiving relationship has a stronger association with the caregiver’s well-being than care location.
Challenges to correcting pluralistic ignorance: false consensus effects, competing information environments, and anticipated social conflict
Authors: Dixon Graham N , Lerner Blue , Bashian Samuel 
Published: February 2024

Abstract
For many policy issues, people holding the majority opinion often do not act in accordance with their beliefs. While underestimating public opinion appears as a likely cause, correcting this misperception often fails to motivate those in the majority to act. Investigating further, we surveyed a nationally representative sample (N = 1,000) of Republican voters about vaccination. Despite a majority supporting vaccines, Republicans on average underestimated other Republicans’ support. However, this misperception occurred primarily among anti-vaccine Republicans—a group that reported a greater willingness to share their vaccine views. We show how an information environment overrepresented with minority views may discourage majority view holders from speaking out even when they are aware of their majority status. That is, instead of experiencing pluralistic ignorance, those in the majority may be discouraged from expressing their views due to anticipated social conflict from engaging in an information environment disproportionately made up of minority views.
Unravelling collaborative governance dynamics within healthcare networks: a scoping review
Authors: Belrhiti Zakaria , Bigdeli Maryam , Lakhal Aniss , Kaoutar Dib , Zbiri Saad , Belabbes Sanaa 
Published: February 2024

Abstract
In many countries, healthcare systems suffer from fragmentation between hospitals and primary care. In response, many governments institutionalized healthcare networks (HN) to facilitate integration and efficient healthcare delivery. Despite potential benefits, the implementation of HN is often challenged by inefficient collaborative dynamics that result in delayed decision-making, lack of strategic alignment and lack of reciprocal trust between network members. Yet, limited attention has been paid to the collective dynamics, challenges and enablers for effective inter-organizational collaborations. To consider these issues, we carried out a scoping review to identify the underlying processes for effective inter-organizational collaboration and the contextual conditions within which these processes are triggered. Following appropriate methodological guidance for scoping reviews, we searched four databases [PubMed (n = 114), Web of Science (n = 171), Google Scholar (n = 153) and Scopus (n = 52)] and used snowballing (n = 22). A total of 37 papers addressing HN including hospitals were included. We used a framework synthesis informed by the collaborative governance framework to guide data extraction and analysis, while being sensitive to emergent themes. Our review showed the prominence of balancing between top-down and bottom-up decision-making (e.g. strategic vs steering committees), formal procedural arrangements and strategic governing bodies in stimulating participative decision-making, collaboration and sense of ownership. In a highly institutionalized context, the inter-organizational partnership is facilitated by pre-existing legal frameworks. HN are suitable for tackling wicked healthcare issues by mutualizing resources, staff pooling and improved coordination. Overall performance depends on the capacity of partners for joint action, principled engagement and a closeness culture, trust relationships, shared commitment, distributed leadership, power sharing and interoperability of information systems To promote the effectiveness of HN, more bottom-up participative decision-making, formalization of governance arrangement and building trust relationships are needed. Yet, there is still inconsistent evidence on the effectiveness of HN in improving health outcomes and quality of care.