bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-breeding of Tenebrio molitor strains from a large-scale perspective
Authors: Adamaki-Sotiraki C. , Deruytter D. , Rumbos C.I. , Athanassiou C.G. 
Published: November 2023

Abstract
To meet the increased future needs in insect Processed Animal Proteins PAPs, insect sector has to optimize the mass rearing of insects. That being the case, a means to tackle this growing demand for insects is to invest in breeding strategies aiming for the production of hybrids with improved economically and biologically valuable traits. In this framework, the objective of the present study was to evaluate the mating compatibility among four strains of the yellow mealworm, Tenebrio molitor, from different geographical areas (Greece, Belgium, Italy and USA) and to assess and compare the performance of the produced inbred and partially outbred lines. All trials were performed in crates of the size (40 × 60 cm) typically used in industrial rearing of mealworm. Adults of T. molitor were placed in the crates and were left to mate and oviposit. To assess mating compatibility, the amount of oviposited eggs (g) as well as the larval hatching rate were recorded. For the assessment of larval performance of inbred and partially outbred lines, two to three weeks old larvae were placed in crates together with Insectus as feeding substrate. The results showed that all crossed lines demonstrated compatibility, and certain combinations outperformed others as suggested by the high cumulative number of eggs and the larval hatch rates for both inbred and partially outbred lines produced. For hatched larvae, there were significant differences among the inbred and partially outbred lines. The highest hatch rates recorded for the Italian-Italian, Belgian-Italian, and Belgian-USA lines. Concerning offspring performance, all crosses followed a similar pattern in terms of final larval weight. The present study aims to draw the attention of the scientific community and insect producing companies to cross-breeding practices to start unfolding its potential for the genetical improvement of commercially farmed insects.
Ethnobiology of edible palm weevil larvae Rhynchophorus palmarum L. (Curculionidae, Coleoptera), a common food source in Amazonian Ecuador
Authors: Jaramillo-Vivanco T. , Cámara R.M. , Cámara M. , Tejera E. , Balslev H. , Álvarez-Suarez J.M. 
Published: November 2023

Abstract
Since ancient times, indigenous communities have depended on traditional and sustainable use of available food sources. Throughout the tropics, the larvae of the palm weevil Rhynchophorus palmarum L. (Curculionidae, Coleoptera) have served this purpose. Several studies have described the ancestral use of R. palmarum as an edible and medicinal insect in the Americas. The use of the grub for food and a system for rearing them are byproducts of the use of palms among several ethnic groups, many of which rely on the same harvesting techniques. The grubs also are used as treatments for several conditions. To better characterise these applications, we developed semi-structured surveys with 58 mostly open-ended questions focused on habitat, food, medicinal use, and commerce of grubs. Respondents were 42 informants from five ethnic groups in Amazonian Ecuador. Analysis indicated that the five groups had similar patterns of use, rearing, and collection of the larvae, but without a structured management plan. Grub collection remains a side activity related to traditional use of palms, but the larvae of this species could offer new opportunities in breeding, commercialisation, and consumption of edible insects as part of a sustainable-use strategy to strengthen food security in Amazonian populations. The potential of the larvae in medicinal use and as a factor in tourism in these regions suggests untapped opportunities with this year-round forest resource.
Mealworms for food and feed: are they only yellow?
Authors: Rumbos C.I. , Athanassiou C.G. 
Published: November 2023

Abstract
Among edible insects, mealworms have attracted a lot of scientific and commercial attention as a nutrient source in food and feed during the last decade. However, the main focus has been on the yellow mealworm, Tenebrio molitor, which undisputedly possesses numerous advantages that render it ideal for mass production. Apart from the yellow mealworm though, other mealworm species have great potential for food and feed applications. The lesser mealworm and the superworm are two species that, although less studied, show promise and could gain their share of the edible insect market in the near future. The present publication aims to put the main edible mealworm species under the spotlight and give a brief overview of their potential as food and feed source.
The Demographic Ceiling of Changes Driven by the Umbrella Movement
Authors: Blenkitni Ariel 
Published: November 2023

Abstract
This article explains post-Umbrella Movement shifts in public opinion in Hong Kong by correlating demographic subgroups with changes in support for the status quo, utilizing Asian Barometer Survey data regarding support for the existing system of government and idealized models for the future. The aim is to determine whether public opinion shifts occurred evenly through population subgroups or were limited to participants and peers. The findings show that while Hong Kongers’ views underwent a seismic shift, the expansion of dissent was unevenly distributed across demographic cohorts, rather than representing demographically symmetric diffusion reflecting societal bridging and cross-demographic coalition building. This indicates that, while the social movement may have shifted aggregate public opinion away from the status quo, its precepts permeated differently across age, class, and especially gender lines, and that views on the status quo were also strongly linked to idealized models for their future, relative to China.
A mobile health intervention for improving the technique of inhaled medications among children with asthma: A pilot study
Authors: Smith Mary Jane , Gao Zhiwei , Chafe Roger , Alwashmi Meshari 
Published: November 2023

Objective BreatheSuite MDI is a Bluetooth-enabled inhaler attachment and mobile application which aims to improve asthma control. The objective was to compare pressurized metered dose inhaler (pMDI) technique and asthma control test (ACT) scores pre- and post-use of the device and mobile application. Secondary objectives were to assess user satisfaction and therapy adherence. Methods Patients between the ages of 8 and 18 were recruited from several pediatric asthma clinics. Technique and ACT scores were assessed at baseline. Users were given no prompts on technique during the first month of device use. For the subsequent three months, users were given technique scores through the mobile application after each inhaler use and provided weekly performance summaries. At the end of the study, technique and ACT scores were analyzed and an exit survey was completed. Conditional logistic regression was used to examine the association between well-controlled asthma (ACT score > 19) and the intervention. Results 24 patients completed the study. Technique scores improved following the use of Breathesuite (44.19 vs. 62.54; P = 0.01). Well-controlled asthma did not significantly improve (OR = 1.20 [0.4–3.9], P = 0.76). 87% of study subjects agreed or strongly agreed that their asthma control improved while using BreatheSuite; 79% were satisfied with the device and mobile application; and 91% preferred using the device compared to a standard logbook to track inhaler usage. Conclusions In this pilot study, the use of BreatheSuite device was associated with improved technique scores. These results need to be confirmed by a randomized controlled trial. There was high user satisfaction with the BreatheSuite device.
Characterization of refractory bricks from selected Cameroonian kaolins
Authors: Pountouenchi Amadou , Njoya Andre , Mbey Jean Aimé , Mache Jacque Richard , Njoya Dayirou , Yongue Fouateu Rose , Njopwouo Daniel , Fagel Nathalie , Pilate Pascal , Van Parys Laurent 
Published: November 2023

AbstractThree clay materials (codded MY3, KG and KK) from western Cameroon were selected and investigated for the production of refractory bricks. Samples MY3 and KG are kaolinite-rich materials, having clay mineral contents of 88% and 72%, respectively, whereas KK is a sand-rich material with a total clay content of 44%. Chamottes were prepared using each clay, and the ground chamottes were later used in the refractory formulation, with each raw clay used as a binding phase. After firing, the X-ray diffraction analyses of all of the refractory bricks indicated the same mineral assemblage made of quartz, cristobalite and mullite. The cristobalite is due to high-temperature conversion of quartz, whereas the mullite is due to clay mineral conversion. The evaluation of the linear shrinkage, physical properties (including bulk density and open porosity) and mechanical testing through refractoriness under load and compressive strength indicates that all of these clays could be used as raw materials for standard clay refractory materials. These results stand as a proof for the potential for these locally available materials to be used as raw materials for refractories that could be locally produced to reduce the cost of access to refractory raw materials faced by industries located in Cameroon.
Pralatrexate injection combined with CHOP for treatment of PTCL: results from the Fol-CHOP dose-finding phase 1 trial
Authors: Iyer Swaminathan P. , Johnston Patrick B. , Barta Stefan K. 
Published: November 2023

Abstract
Pralatrexate is a folate antagonist that selectively enters cells expressing reduced folate carrier type 1 and competitively inhibits dihydrofolate reductase, leading to interruption of RNA synthesis, DNA replication, and apoptosis. This phase 1 study was conducted to evaluate the maximum tolerated dose (MTD) of pralatrexate in combination with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) regimen (part 1) and the response and pharmacokinetics of 6 cycles of this combination (CHOP + Folotyn 30 mg/m2 [Fol-CHOP]) in patients with newly diagnosed peripheral T-cell lymphoma (PTCL). In part 1, on days 1 and 8 of each cycle, patients were treated with 10, 15, 20, 25, or 30 mg/m2 of pralatrexate in combination with CHOP, per dose escalation, in 5 sequential cohorts. No patients experienced DLTs in cohorts 1, 2, 3, 4, and 5. The pralatrexate dose of 30 mg/m2 was selected to be combined with CHOP for part 2 and administered to 33 additional patients in the expansion cohort. At the MTD, the Fol-CHOP regimen was generally well tolerated in patients with PTCL, with an overall response rate (ORR) of 83.9% (20 complete response and 6 partial response), as assessed by treating investigators. Thirty-five patients (67.3%) experienced grade 3/4 treatment-emergent adverse events, the most common of which were anemia (21.2%), neutropenia (19.2%), febrile neutropenia (11.5%), fatigue, mucosal inflammation, nausea, and vomiting (7.7% each). In conclusion, Fol-CHOP was found to be a safe and effective treatment for newly diagnosed PTCL and deemed worthy of further investigation. This trial was registered at www.ClinicalTrials.gov as #NCT02594267.
Functional relevance of circRNA aberrant expression in pediatric acute leukemia with KMT2A::AFF1 fusion
Authors: Tretti Parenzan Caterina , Molin Anna Dal , Longo Giorgia , Gaffo Enrico , Buratin Alessia , Cani Alice , Boldrin Elena , Serafin Valentina , Guglielmelli Paola , Vannucchi Alessandro M. , Cazzaniga Giovanni , Biondi Andrea , Locatelli Franco , Meyer Lueder H. , Buldini Barbara , te Kronnie Geertruij , Bresolin Silvia , Bortoluzzi Stefania 
Published: November 2023

Abstract
Circular RNAs (circRNAs) are emerging molecular players in leukemogenesis and promising therapeutic targets. In KMT2A::AFF1 (MLL::AF4)-rearranged leukemia, an aggressive disease compared with other pediatric B-cell precursor (BCP) acute lymphoblastic leukemia (ALL), data about circRNAs are limited. Here, we disclose the circRNA landscape of infant patients with KMT2A::AFF1 translocated BCP-ALL showing dysregulated, mostly ectopically expressed, circRNAs in leukemia cells. Most of these circRNAs, apart from circHIPK3 and circZNF609, previously associated with oncogenic behavior in ALL, are still uncharacterized. An in vitro loss-of-function screening identified an oncogenic role of circFKBP5, circKLHL2, circNR3C1, and circPAN3 in KMT2A::AFF1 ALL, whose silencing affected cell proliferation and apoptosis. Further study in an extended cohort disclosed a significantly correlated expression of these oncogenic circRNAs and their putative involvement in common regulatory networks. Moreover, it showed that circAFF1 upregulation occurs in a subset of cases with HOXA KMT2A::AFF1 ALL. Collectively, functional analyses and patient data reveal oncogenic circRNA upregulation as a relevant mechanism that sustains the malignant cell phenotype in KMT2A::AFF1 ALL.
Linking GATA2 to myeloid dysplasia and complex cytogenetics in adult myelodysplastic neoplasm and acute myeloid leukemia
Authors: Robbins Daniel J. , Pavletich Tatiana S. , Patil Apoorva T. , Pahopos Demetra , Lasarev Michael , Polaki Usha S. , Gahvari Zhubin J. , Bresnick Emery H. , Matson Daniel R. 
Published: November 2023

Abstract
GATA binding protein 2 (GATA2) is a conserved zinc finger transcription factor that regulates the emergence and maintenance of complex genetic programs driving development and function of hematopoietic stem and progenitor cells (HSPCs). Patients born with monoallelic GATA2 mutations develop myelodysplastic neoplasm (MDS) and acute myeloid leukemia (AML), whereas acquired GATA2 mutations are reported in 3% to 5% of sporadic AML cases. The mechanisms by which aberrant GATA2 activity promotes MDS and AML are incompletely understood. Efforts to understand GATA2 in basic biology and disease will be facilitated by the development of broadly efficacious antibodies recognizing physiologic levels of GATA2 in diverse tissue types and assays. Here, we purified a polyclonal anti-GATA2 antibody and generated multiple highly specific anti-GATA2 monoclonal antibodies, optimized them for immunohistochemistry on patient bone marrow bioosy samples, and analyzed GATA2 expression in adults with healthy bone marrow, MDS, and acute leukemia. In healthy bone marrow, GATA2 was detected in mast cells, subsets of CD34+ HSPCs, E-cadherin–positive erythroid progenitors, and megakaryocytes. In MDS, GATA2 expression correlates with bone marrow blast percentage, positively correlates with myeloid dysplasia and complex cytogenetics, and is a nonindependent negative predictor of overall survival. In acute leukemia, the percent of GATA2+ blasts closely associates with myeloid lineage, whereas a subset of lymphoblastic and undifferentiated leukemias with myeloid features also express GATA2. However, the percent of GATA2+ blasts in AML is highly variable. Elevated GATA2 expression in AML blasts correlates with peripheral neutropenia and complex AML cytogenetics but, unlike in MDS, does not predict survival.
Is moderate resistance training adequate for older adults with sarcopenia? A systematic review and network meta-analysis of RCTs
Authors: Chen Yu Chang , Chen Wang-Chun , Liu Chia-Wei , Huang Wei-Yu , Lu ICheng , Lin Chi Wei , Huang Ru Yi , Chen Jung Sheng , Huang Chi Hsien 
Published: November 2023

Abstract
Background
Resistance training (RT) and nutritional supplementation are recommended for the management of sarcopenia in older adults. However, optimal RT intensity for the treatment of sarcopenia has not been well investigated.

Methods
This network meta-analysis aims to determine the comparative effectiveness of interventions for sarcopenia, taking RT intensity into consideration. RT intensity was classified into light-to-moderate intensity RT(LMRT), moderate intensity RT(MRT), and moderate-to-vigorous intensity RT(MVRT) based on percentage of one repetition maximum (%1RM) and/or rating of perceived exertion.

Results
A total of 50 RCTs (N = 4,085) were included after screening 3,485 articles. The results confirmed that RT with or without nutrition was positively associated with improved measures of muscle strength and physical performance. Regarding RT intensity, LMRT only demonstrated positive effects on hand grip (aerobic training + LMRT + nutrition: mean difference [MD] = 2.88; 95% credential intervals [CrI] = 0.43,5.32). MRT provided benefits on improvement in the 30-s chair stand test (repetitions) (MRT: MD = 2.98, 95% CrI = 0.35,5.59), timed up and go test (MRT: MD = -1.74, 95% CrI: = -3.34,-0.56), hand grip (MRT: MD = 2.44; 95% CrI = 0.03,5.70), and leg press (MRT: MD = 8.36; 95% CrI = 1.87,13.4). MVRT also improved chair stand test repetitions (MVRT: MD = 5.64, 95% CrI = 0.14,11.4), gait speed (MVRT + nutrition: MD = 0.21, 95% CrI = 0.003,0.48), appendicular skeletal muscle index (MVRT + nutrition: MD = 0.25, 95% CrI = 0.01,0.5), and leg press (MVRT: MD = 14.7, 95% CrI: 5.96,22.4; MVRT + nutrition: MD = 17.8, 95% CrI: 7.55,28.6).

Conclusion
MVRT had greater benefits on muscle mass, lower extremity strength, and physical performance compared to MRT. Increasing RT intensity may be recommended for sarcopenic older adults.
Self-discharge during treatment for acute recreational drug toxicity: an observational study from emergency departments in seven European countries
Authors: Vallersnes Odd Martin , Dines Alison M. , Wood David M. , Heyerdahl Fridtjof , Hovda Knut Erik , Yates Christopher , Giraudon Isabelle , Caganova Blazena , Ceschi Alessandro , Galicia Miguel , Liakoni Evangelia , Liechti Matthias E. , Miró Òscar , Noseda Roberta , Persett Per Sverre , Põld Kristiina , Schmid Yasmin , Scholz Irene , Vigorita Federico , Dargan Paul I. 
Published: November 2023

Abstract
Background
Self-discharge is a risk factor for readmission and excess mortality. We assess the rate of self-discharge from the emergency department (ED) among presentations for acute recreational drug toxicity and identify factors associated with self-discharge.

Methods
From the Euro-DEN Plus database of presentations to the ED with acute recreational drug toxicity, we extracted data from 11 centres in seven European countries from 2014 to 2017. Self-discharge was defined as taking one’s own discharge or escaping from the ED before being medically cleared. We used multiple logistic regression analyses to look for factors associated with self-discharge.

Results
Among 15,135 included presentations, 1807 (11.9%) self-discharged. Self-discharge rates varied from 1.7 to 17.1% between centres. Synthetic cannabinoids were associated with self-discharge, adjusted odds ratio 1.44 (95% confidence interval 1.10–1.89), as were heroin, 1.44 (1.26–1.64), agitation, 1.27 (1.10–1.46), and naloxone treatment, 1.27 (1.07–1.51), while sedation protected from self-discharge, 0.38 (0.30–0.48).

Conclusion
One in eight presentations self-discharged. There was a large variation in self-discharge rates across the participating centres, possibly partly reflecting different discharge procedures and practices. Measures to improve the management of agitation and cautious administration of naloxone to avoid opioid withdrawal symptoms may be approaches worth exploring to reduce self-discharge.
Biocaiv: an integrative webserver for motif-based clustering analysis and interactive visualization of biological networks
Authors: Li Dong-Xu , Zhou Peng , Zhao Bo-Wei , Su Xiao-Rui , Li Guo-Dong , Zhang Jun , Hu Peng-Wei , Hu Lun 
Published: November 2023

Abstract
Background
As an important task in bioinformatics, clustering analysis plays a critical role in understanding the functional mechanisms of many complex biological systems, which can be modeled as biological networks. The purpose of clustering analysis in biological networks is to identify functional modules of interest, but there is a lack of online clustering tools that visualize biological networks and provide in-depth biological analysis for discovered clusters.

Results
Here we present BioCAIV, a novel webserver dedicated to maximize its accessibility and applicability on the clustering analysis of biological networks. This, together with its user-friendly interface, assists biological researchers to perform an accurate clustering analysis for biological networks and identify functionally significant modules for further assessment.

Conclusions
BioCAIV is an efficient clustering analysis webserver designed for a variety of biological networks. BioCAIV is freely available without registration requirements at http://bioinformatics.tianshanzw.cn:8888/BioCAIV/.
Cystatin C-derived estimated glomerular filtration rate in children with sickle cell anaemia
Authors: Babatunde Hakeem Edun , Bello Afeez Oyesola , Adeboye Muhammed A. Nurudeen , Folayan Olumuyiwa Shola , Ojewole Olugoke Ezekiel , Abubakar Usman 
Published: November 2023

Abstract
Background
Sickle cell disease is the most common inherited blood disorder in humans and constitutes a major public health burden. It is a multisystemic condition with long-term renal complications. Early detection of sickle cell nephropathy and initiation of appropriate interventions are associated with improved survival and quality of life. This study aimed to compare the cystatin C-derived estimated glomerular filtration rate (GFR) of the study groups and also, to correlate the clinical features of chronic kidney disease (CKD) with decreased GFR in children with sickle cell anaemia (SCA).

Methods
This hospital-based cross-sectional analytic study recruited 86 SCA subjects in steady-state and 86 age and sex-matched healthy HbAA controls aged 1–14 years who attended the Paediatric Haematology and Outpatient clinics of Federal Medical Centre Bida over six months. Data were collected using a semi-structured questionnaire, and participants’ length/height, weight, and blood pressure were measured using standard procedures. Blood samples were drawn for serum cystatin C assay via the sandwich enzyme-linked immunosorbent assay (ELISA) technique. Filler’s equation was used to calculate the glomerular filtration rate.

Results
There was a significant difference in the mean cystatin C-derived GFR between the two groups, i.e. 116 ± 30mL/min/1.73m2 vs. 106 ± 24mL/min/1.73m2 for the SCA and control groups, respectively (p = 0.017). The prevalence of supernormal GFR (i.e. GFR > 140mL/min/1.73m2) and decreased GFR (i.e. GFR < 90mL/min/1.73m2) was 19.8% and 22.1%, respectively, in children with SCA. There was no significant association between the age at diagnosis of SCA, blood transfusions, blood pressure, packed cell volume and presence of peripheral oedema with decreased GFR in the study subjects.

Conclusions
Supernormal GFR is common in children with SCA and there is no significant association between clinical features of CKD with decreased GFR. Regular evaluation of renal function is, however, recommended in children with SCA for early detection and treatment of renal complications in order to halt the progression to end-stage kidney disease (ESKD).