bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in Weather Systems during Anomalously Wet and Dry Years in Southeastern Australia
Authors: Jin Chenhui , Reeder Michael J. , Gallant Ailie J. E. , Parker Tess , Sprenger Michael 
Published: November 2023

Abstract
This study focuses on the rainfall-producing weather systems in the southern Murray–Darling Basin (MDB), Australia. These weather systems are divided into objects: cyclones, fronts, anticyclones, warm conveyor belt (WCB) inflows, WCB ascents, potential vorticity (PV) streamers, and cutoff lows. We investigate the changes in the frequency, amplitude, and relative position of these objects as the daily and seasonal rainfall change. Days on which the rainfall is heavy, especially in winter, are characterized by more PV streamers, cutoff lows, cyclones, fronts, and WCBs in the region. In contrast, dry days are characterized by more anticyclones over southeastern Australia in winter and summer. The effect of upper-level weather objects (PV streamers and cutoff lows) on lower-level objects, and their importance in producing rainfall, is quantified using the quasigeostrophic ω equation and separating the vertical motion into that induced by the upper and lower levels. On heavy rainfall days in winter, PV streamers and cutoff lows force strong upward motion in the lower troposphere, promoting cyclogenesis at lower levels, forcing ascent in the WCBs, and producing rain downstream of the southern MDB. Lower-level ascent forced by upper-level objects is important for the development of heavy rainfall in both seasons, although particularly in winter. Rainfall is attributed to individual objects. PV streamers and WCBs contribute most to the winter and summer rainfall, respectively. The difference in rainfall between anomalously wet and dry years can be explained in winter by the changes in the rainfall associated with PV streamers, whereas in summer it is mostly due to a reduction in the rainfall associated with WCBs.

Significance Statement
The aim of the present study is to better understand how synoptic-scale weather systems differ in southeastern Australia in dry and wet periods, by considering a wide range of weather systems. We found weather systems are more closely aligned in the vertical on heavy rainfall days, and the majority of rainfall in this region is associated with warm conveyor belts. These results point to warm conveyor belts being an important, but not well recognized, contributor to rainfall in this region. Future work may investigate the roles of the various modes of variability and climate change in modulating warm conveyor belts and hence the regional rainfall variability in Australia.
Reliability of Open Public Electric Vehicle Direct Current Fast Chargers
Authors: Rempel David , Cullen Carleen , Matteson Bryan Mary , Vianna Cezar Gustavo 
Published: November 2023

Objective The aim was to systematically evaluate the usability of all public electric vehicles (EV) direct current fast chargers (DCFC) in the San Francisco region. Background To achieve a rapid transition to EVs, a highly reliable and easy to use charging infrastructure is critical to building confidence among consumers. Methods The functionality and usability of all 182 open, public DCFC charging stations with CCS connectors (combined charging system) in the 9 counties of the Bay Area were tested (655 electric vehicle service equipment (EVSE) ports). An EVSE was classified as functional if it charged an EV for 2 minutes. Results Overall, 73.3% of the 655 EVSEs were functional. The causes of the nonfunctioning EVSEs (23.5%) were blank or unresponsive screens or error messages; payment system failures; charge initiation failures; network failures; or broken connectors. In addition, the cable was too short to reach the EV inlet for 3.2% of the EVSEs. A random sampling of 10% of the EVSEs, approximately 8 days after the first evaluation, found no overall change in functionality. Conclusions The level of functionality found with field testing conflicts with the 95–98% uptime reported by the EV service providers (EVSPs) who operate the EV charging stations. There is a need for precise and verifiable definitions of uptime, downtime, and excluded time, as applied to public EV chargers. Application The level of failure of the existing public EV DCFC charge infrastructure highlights the importance of improving the system design and maintenance to improve adoption of EVs.
Strength-based cognitive-behavioural therapy and peer-to-peer support in the recovery process for people with schizophrenia: A randomised control trial
Authors: Wong Daniel Fu Keung , Cheung Yves Cho Ho , Oades Lindsay G , Ye Shengquan Sam , Ng Yat-nam Petrus 
Published: November 2023

Background and aims: Dysfunctional cognition and negative symptoms in schizophrenia are associated with persistently low social functioning and quality of life (QoL). Recovery interventions report only a modest effect in improving social functioning and QoL. This study examined the therapeutic effects and pathways of interventions using strength-based cognitive-behavioural therapy (SBCBT) and peer-to-peer support (PSP) approaches. Methods: A randomised control trial compared SBCBT, PSP and TAU (treatment-as-usual) by recruiting 127 individuals with schizophrenia spectrum disorders and low social functioning. Results: The three-group comparison found SBCBT produced improved social functioning and social support at post-intervention and 6-month follow-up while PSP reported improved social QoL and defeatist beliefs, but none remained at 6-month follow-up. Unsurprising, no improvement was found in any areas for TAU, both at post-test and 6-month follow-up. Regarding mechanisms of change, improvements in hope and personal recovery consistently and significantly accounted for improvements in social functioning and many aspects of QoL of participants, thus indicating the important contribution of hope and personal recovery in strength-based interventions for people with schizophrenia. Conclusion: This rigorously designed study provides the first few solid empirical evidence on the effectiveness of SBCBT and PSP in recovery from schizophrenia and provides initial evidence of the linkage between dysfunctional cognition, hope and recovery in schizophrenia.
Erastin induces ferroptosis in cervical cancer cells via Nrf2/HO-1 signaling pathway
Authors: Wei Xiaoning , Huang Qiaoqiao , Huang Jinbing , Yu Li , Chen Junying 
Published: November 2023

Objective Our research aims to assess the influence of erastin, a ferroptosis-inducing agent, on cervical cancer cells. Introduction Cervical cancer is a prevalent malignancy in females. Dysregulation of ferroptosis, a form of cell demise reliant on iron, is implicated in several cancers. Methods The effect of erastin on HeLa and SiHa was detected by transwell assay, scratch test, and colony formation assay, while cell apoptosis was detected using flow cytometry. Cellular reactive oxygen species (ROS) generation was detected using the dichloro-dihydro-fluorescein diacetate assay. Sequencing analysis identified differentially expressed genes (DEGs), and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Enrichment analyses were employed to identify the target gene. Subsequently, the utilization of small interfering RNA (siRNA) was employed to suppress the targeted gene expression in HeLa cells, thereby effectively mitigating the impact of erastin on various cellular processes including invasion, colony formation, migration, and ROS generation. Results The findings indicate that erastin attenuates the viability of both HeLa cells (IC50 = 30.88 µM) and SiHa cells (IC50 = 29.40 µM). Treatment with erastin at 10 µM inhibits the invasion, colony formation, and migration of both HeLa and SiHa cells within 24 h. Ferrostatin-1 (1 µM) notably alleviates the inhibitory effects of erastin of HeLa and SiHa cells. Upregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream target, heme oxygenase-1 (HO-1), was found in erastin-treated cells compared to the control group. When knocked down HO-1 in HeLa cells, effectively counteracting the effects of erastin on the invasion, colony formation, migration, and ROS production in HeLa cells. Conclusion Our research demonstrates that erastin induces ferroptosis and the accumulation of ROS in cervical cancer cells by activating the Nrf2/HO-1 pathway, significantly reducing cell proliferation and motility. These findings propose a potential molecular mechanism of erastin-mediated cervical cancer development.
Nrf2 attenuates methamphetamine-induced myocardial injury by regulating oxidative stress and apoptosis in mice
Authors: Yu Hao , Peng Yanxia , Dong Wenjuan , Shen Baoyu , Yang Genmeng , Nie Qianyun , Tian Yan , Qin Lixiang , Song Chunhui , Chen Bingzheng , Zhao Yongna , Li Lihua , Hong Shijun 
Published: November 2023

Objectives Methamphetamine (MA) abuse is a serious social problem worldwide. Cardiovascular complications were the second leading cause of death among MA abusers. We aimed to clarify the effects of MA on myocardial injury, oxidative stress, and apoptosis in myocardial cells and to explore the potential mechanism of nuclear factor-erythroid factor 2-related factor 2 (Nrf2) in MA-induced oxidative stress and apoptosis. Methods An acute cardiac toxicity model of MA was established by intraperitoneal injection of MA (2 mg/kg) for 5 days. Nrf2 activation (by sulforaphane (SFN) 1 h before MA injection) and Nrf2 gene knockout were performed to explore the regulatory effects of Nrf2 on cardiac toxicity. Results The protein expressions of Nrf2 ( p < .001) and heme oxygenase-1 (HO-1) were increased ( p < .01), suggesting that MA activated the Nrf2/HO-1 pathway. In the MA group, cardiac injury score ( p < .001) and cardiac troponin I (cTnI) protein expression increased ( p < .01). Malondialdehyde (MDA) content increased ( p < .001), superoxide dismutase (SOD) activity decreased ( p < .05). Protein expressions of Caspase-3 ( p < .001) and Bax ( p < .001) increased, and Bcl-2 decreased ( p < .001) as well. These changes were reversed by activation of Nrf2 but became more pronounced after Nrf2 knockout, suggested that the activation and knockout of Nrf2 attenuated and aggravated MA-induced myocardial injury, oxidative stress and apoptosis in myocardial cells, respectively. Conclusions MA administration induced myocardial injury, oxidative stress, and apoptosis in mice. Nrf2 attenuated MA-induced myocardial injury by regulating oxidative stress and apoptosis, thus playing a protective role.
Waitlist-controlled trial of an online intervention to address mental health among older people living with HIV
Authors: Berko Jeff , Mazonson Peter , Short Duncan , Karris Maile , Ehui Lynsay , Gutner Cassidy A , Spinelli Frank , Zolopa Andrew 
Published: November 2023

Background: Older people living with HIV (PLWH) often experience elevated levels of depression, anxiety, and loneliness. Methods: This waitlist-controlled trial examined the effectiveness of online audio mindfulness lessons in impacting these feelings among older PLWH. Results: Among 214 participants, the mean (SD) age was 60.4 (5.9) years, 89% were male, and 69% were white. After 25 days, the intervention group showed significant improvements versus the waitlist control group in symptoms of depression (20.3% improvement, p < .01) and symptoms of anxiety (22.4% improvement, p = .03), but not in loneliness as measured by a Daily Diary (12.9% improvement, p = .07) or the 3-Item Loneliness Scale (4.8% improvement, p = .27). Secondary analyses among participants with elevated baseline symptoms of depression showed a 26.3% improvement ( p < .01), with a moderate effect size (Hedge’s g = 0.69). Similarly, those with elevated baseline symptoms of anxiety showed a 25.6% improvement ( p < .01), a moderate effect size (g = 0.54), while those with moderate or severely elevated loneliness showed an 18.9% improvement in daily loneliness ( p < .01), a moderate effect size (g = 0.55). Conclusion: This waitlist-controlled trial is the first to show that a series of brief, online audio mindfulness lessons improves mental health outcomes among older PLWH. For many patients, this intervention may offer relief that is both accessible and affordable.
Ultrasound-Assessed Lesion Morphology and Drug-Coated Balloon Treatment for de novo Dysfunctional Arteriovenous Fistula in Hemodialysis Patients
Authors: Suemitsu Kotaro , Shiraki Tatsuya , Iida Osamu , Oka Kanako , Ota Naomi , Izumi Masaaki 
Published: November 2023

Aim: This study aimed to evaluate the effect of ultrasound-assessed lesion morphology on the outcomes of drug-coated balloon (DCB) versus plain old balloon angioplasty (POBA) treatment for de novo dysfunctional arteriovenous fistulas (AVF) lesions. Methods: This single-center retrospective study enrolled 114 consecutive patients (mean age, 73 ± 10 years; male, 69%) with de novo dysfunctional AVF lesions who underwent percutaneous transluminal angioplasty (PTA) using DCB (n = 48) and POBA (n = 66). The morphology of the stenotic lesions, evaluated using ultrasonography, was classified into intimal hyperplasia and shrinking types. The outcome measure was 12-month primary patency. Factors associated with loss of primary patency were evaluated using Cox proportional hazards models. Results: The baseline characteristics were not significantly different between the 2 treatment groups. The 12-month primary patency rate was significantly higher in the DCB group than in the POBA group (66.8 ± 7.1% versus 35.9 ± 6.3%, P = .006). The 12-month primary patency rate in the lesions with intimal hyperplasia type was not significantly different (DCB: 70.3 ± 9.5% versus POBA: 45.9 ± 8.0%; P = .310), whereas that in the shrinking type was significantly higher in the DCB group than in the POBA group (61.9 ± 10.6% versus 15.2 ± 8.1%; P < .001). The interaction analysis demonstrated that lesion morphology had a significantly different hazard ratio (HR) for restenosis between the POBA and DCB groups (P for interaction = .031). The multivariate analysis revealed that DCB usage (adjusted hazard ratio [aHR], 0.49; 95% confidence interval [CI]: [0.28, 0.87]; P = .015), ultrasound-assessed lesion morphology (shrinking type: aHR, 1.77; 95% CI: [1.07, 2.93]; P = .026), and location of stenosis (aHR, 2.26; 95% CI: 1.15, 4.46; P = .018) were significantly associated with AVF patency after PTA. Conclusion: This study revealed that lesion morphology evaluated using ultrasonography had a differential impact on DCB and POBA outcomes. The therapeutic effect of DCB was unexpectedly confirmed in the shrinking type. Clinical Impact The effectiveness of DCB in inhibiting smooth muscle cell proliferation in intimal hyperplasia lesions was expected based on the known mechanism of action of paclitaxel. However the therapeutic effect of DCB was unexpectedly confirmed in the shrinking type too. We may not need to hesitate usage of DCB for shrinking type.
Stroke characteristics and outcomes in urban Tanzania: Data from the Prospective Lake Zone Stroke Registry
Authors: Matuja Sarah Shali , Ngimbwa Joshua , Andrew Lilian , Shindika Jemima , Nchasi Goodluck , Kasala Anna , Paul Innocent Kitandu , Ndalahwa Mary , Mawazo Akili , Kalokola Fredrick , Ngoya Patrick , Rudovick Ladius , Kilonzo Semvua , Wajanga Bahati , Massaga Fabian , Kalluvya Samuel E , Munseri Patricia , Mnacho Mohamed A , Okeng’o Kigocha , Kimambo Henrika , Manji Mohamed , Ruggajo Paschal , Nagu Tumaini , Ahmed Rashid Ali , Sheriff Faheem , Mahawish Karim , Mangat Halinder , Nguyen-Huynh Mai N , Saylor Deanna , Peck Robert 
Published: November 2023

Background: Stroke is a second leading cause of death globally, with an estimated one in four adults suffering a stroke in their lifetime. We aimed to describe the clinical characteristics, quality of care, and outcomes in adults with stroke in urban Northwestern Tanzania. Methods: We analyzed de-identified data from a prospective stroke registry from Bugando Medical Centre in Mwanza, the second largest city in Tanzania, between March 2020 and October 2022. This registry included all adults ⩾18 years admitted to our hospital who met the World Health Organization clinical definition of stroke. Information collected included demographics, risk factors, stroke severity using the National Institutes of Health Stroke Scale, brain imaging, indicators for quality of care, discharge modified Rankin Scale, and in-hospital mortality. We examined independent factors associated with mortality using logistic regression. Results: The cohort included 566 adults, of which 52% (294) were female with a mean age of 65 ± 15 years. The majority had a first-ever stroke 88% (498). Premorbid hypertension was present in 86% (488) but only 41% (200) were taking antihypertensive medications before hospital admission; 6% (32) had HIV infection. Ischemic strokes accounted for 66% (371) but only 6% (22) arriving within 4.5 h of symptom onset. In-hospital mortality was 29% (127). Independent factors associated with mortality were severe stroke (adjusted odds ratio (aOR) = 1.81, 95% confidence interval (CI) = 1.47–2.24, p < 0.001), moderate to severe stroke (aOR = 1.49, 95% CI = 1.22–1.84, p < 0.001), moderate stroke (aOR = 1.80, 95% CI = 1.52–2.14, p < 0.001), leukocytosis (aOR = 1.19, 95% CI = 1.03–1.38, p = 0.022), lack of health insurance coverage (aOR = 1.15, 95% CI = 1.02–1.29, p = 0.025), and not receiving any form of venous thromboembolism prophylaxis (aOR = 1.18, 95% CI = 1.02–1.37, p = 0.027). Conclusion: We report a stroke cohort with poor in-hospital outcomes in urban Northwestern Tanzania. Early diagnosis and treatment of hypertension could prevent stroke in this region. More work is needed to raise awareness about stroke symptoms and to ensure that people with stroke receive guidelines-directed therapy.
Factors influencing study outcomes in recent literature on distal radial fracture treatment
Authors: Akgülle Ahmet Hamdi , Uysal Dilara , Bekiroğlu Gülnaz Nural 
Published: November 2023

The main tools used by an orthopaedic surgeon for managing distal radial fracture treatment are recent literature and treatment guidelines. The aim of the present study was to find which factors within the study design influence study outcomes the most. Trials in three major databases (PubMed, Scopus, Embase) comparing surgical and non-surgical treatment options for adolescent and adult distal radial fractures with their original data, between 2013 and 2021, were included. The selected 47 studies were classified according to their outcomes. The relationship between study characteristics and outcomes was statistically analysed. It was more likely to find no difference in outcomes between volar locking plate and less invasive treatments when the sample size was above 100, follow-up was more than 1 year and functional assessments were used. A small sample size and short follow-up time affect study outcomes in favour of a volar locking plate. Readers should focus on the design criteria and read the full text of the studies before making any conclusions. Level of evidence III
Deciphering and disrupting PIEZO1-TMEM16F interplay in hereditary xerocytosis
Authors: Liang Pengfei , Zhang Yang , Wan Yui Chun S. , Ma Shang , Dong Ping , Lowry Augustus J. , Francis Samuel J. , Khandelwal Sanjay , Delahunty Martha , Telen Marilyn J. , Strouse John J. , Arepally Gowthami M. , Yang Huanghe 
Published: November 2023

Abstract
Cell-surface exposure of phosphatidylserine (PS) is essential for phagocytic clearance and blood clotting. Although a calcium-activated phospholipid scramblase (CaPLSase) has long been proposed to mediate PS exposure in red blood cells (RBCs), its identity, activation mechanism, and role in RBC biology and disease remain elusive. Here, we demonstrate that TMEM16F, the long-sought-after RBC CaPLSase, is activated by calcium influx through the mechanosensitive channel PIEZO1 in RBCs. PIEZO1-TMEM16F functional coupling is enhanced in RBCs from individuals with hereditary xerocytosis (HX), an RBC disorder caused by PIEZO1 gain-of-function channelopathy. Enhanced PIEZO1-TMEM16F coupling leads to an increased propensity to expose PS, which may serve as a key risk factor for HX clinical manifestations including anemia, splenomegaly, and postsplenectomy thrombosis. Spider toxin GsMTx-4 and antigout medication benzbromarone inhibit PIEZO1, preventing force-induced echinocytosis, hemolysis, and PS exposure in HX RBCs. Our study thus reveals an activation mechanism of TMEM16F CaPLSase and its pathophysiological function in HX, providing insights into potential treatment.
Micro-genetic environmental sensitivity across macro-environments of chickens reared in Burkina Faso and France
Authors: Madsen Mette Dam , Duijvesteijn Naomi , van der Werf Julius , Clark Sam 
Published: November 2023

AbstractBackgroundCommercial poultry production systems follow a pyramidal structure with a nucleus of purebred animals under controlled conditions at the top and crossbred animals under commercial production conditions at the bottom. Genetic correlations between the same phenotypes on nucleus and production animals can therefore be influenced by differences both in purebred-crossbred genotypes and in genotype-by-environment interactions across the two environments, known as macro-genetic environmental sensitivity (GES). Within each environment, genotype-by-environment interactions can also occur due to so-called micro-GES. Micro-GES causes heritable variation in phenotypes and decreases uniformity. In this study, genetic variances of body weight (BW) and of micro-GES of BW and the impacts of purebred-crossbred differences and macro-environmental differences on micro-GES of BW were estimated. The dataset contained three subpopulations of slow-growing broiler chickens: purebred chickens (PB) reared in France, and crossbred chickens reared in France (FR) under the same conditions as PB or reared in Burkina Faso (BF) under local conditions. The crossbred chickens were offspring of the same dam line and had PB as their sire line.ResultsEstimates of heritability of BW and micro-GES of BW were 0.54 (SE of 0.02) and 0.06 (0.01), 0.67 (0.03) and 0.03 (0.01), and 0.68 (0.04) and 0.02 (0.01) for the BF, FR, and PB subpopulations, respectively. Estimates of the genetic correlations for BW between the three subpopulations were moderately positive (0.37 to 0.53) and those for micro-GES were weakly to moderately positive (0.01 to 0.44).ConclusionsThe results show that the heritability of the micro-GES of BW varies with macro-environment, which indicates that responses to selection are expected to differ between macro-environments. The weak to moderate positive genetic correlations between subpopulations indicate that both macro-environmental differences and purebred-crossbred differences can cause re-ranking of sires based on their estimated breeding values for micro-GES of BW. Thus, the sire that produces the most variable progeny in one macro-environment may not be the one that produces the most variable offspring in another. Similarly, the sire that produces the most variable purebred progeny may not produce the most variable crossbred progeny. The results highlight the need for investigating micro-GES for all subpopulations included in the selection scheme, to ensure optimal genetic gain in all subpopulations.