bluuebunny/crossref_metadata_2025_split huggingface dataset:
Light‐Driven Metabolic Pathways in Non‐Photosynthetic Biohybrid Bacteria
Authors: Bassett Shane , Ding Yuchen , Roy Micaela K. , Reisz Julie A. , D'Alessandro Angelo , Nagpal Prashant , Chatterjee Anushree 
Published: October 2023

AbstractBiomanufacturing via microorganisms relies on carbon substrates for molecular feedstocks and a source of energy to carry out enzymatic reactions. This creates metabolic bottlenecks and lowers the efficiency for substrate conversion. Nanoparticle biohybridization with proteins and whole cell surfaces can bypass the need for redox cofactor regeneration for improved secondary metabolite production in a non‐specific manner. Here we propose using nanobiohybrid organisms (Nanorgs), intracellular protein‐nanoparticle hybrids formed through the spontaneous coupling of core‐shell quantum dots (QDs) with histidine‐tagged enzymes in non‐photosynthetic bacteria, for light‐mediated control of bacterial metabolism. This proved to eliminate metabolic constrictions and replace glucose with light as the source of energy in Escherichia coli, with an increase in growth by 1.7‐fold in 75 % reduced nutrient media. Metabolomic tracking through carbon isotope labeling confirmed flux shunting through targeted pathways, with accumulation of metabolites downstream of respective targets. Finally, application of Nanorgs with the Ehrlich pathway improved isobutanol titers/yield by 3.9‐fold in 75 % less sugar from E. coli strains with no genetic alterations. These results demonstrate the promise of Nanorgs for metabolic engineering and low‐cost biomanufacturing.
Insight into the Optoelectronic Nature and Mechanical Stability of Binary Chalcogenides: A First‐Principles Study
Authors: Gul Banat , Fayz‐Al‐Asad Md. , Khan Muhammad Salman , Rahaman Mostafizur , Periyasami Govindasami , Ahmad Hijaz 
Published: October 2023

AbstractThe most promising candidates for solar cells and optoelectronic devices are the transition metals dichalcogenides. Here, we employed the well‐known density functional theory to examine the structural, optoelectronic, and elastic characteristics of novel binary chalcogenides. A direct band gap for ZrX2 and an indirect band gap for PtX2 materials were both confirmed by the band structure features. The valence and conduction band regions are formed by the interaction of the A‐d and X‐p bands. Both the formation energy and the cohesive energies are calculated. The phonon dispersion plots confirmed the stability of the structures. Furthermore, the significant optical constants are computed and explained for possible employment in the optoelectronic application. Our computed band gaps and refractive index were found to be inversely associated. The vital elastic properties are also calculated to discuss the mechanical stability of these materials. The greater bulk modulus and Young's modulus for ZrS2 as compared to the studied chalcogenides suggest this material to be harder and more compressible. The B/G values, confirm all the studied dichalcogenides to be ductile. The most ductile of these materials was ZrSe2 with a predicted B/G value of 7.65. The present work could primarily aid in the creation of diverse and potentially useful semiconducting devices and their applications.
Charting the course to solid‐state dual‐ion batteries
Authors: Asfaw Habtom D. , Kotronia Antonia , Garcia‐Araez Nuria , Edström Kristina , Brandell Daniel 
Published: October 2023

AbstractAn electrolyte destined for use in a dual‐ion battery (DIB) must be stable at the inherently high potential required for anion intercalation in the graphite electrode, while also protecting the Al current collector from anodic dissolution. A higher salt concentration is needed in the electrolyte, in comparison to typical battery electrolytes, to maximize energy density, while ensuring acceptable ionic conductivity and operational safety. In recent years, studies have demonstrated that highly concentrated organic electrolytes, ionic liquids, gel polymer electrolytes (GPEs), ionogels, and water‐in‐salt electrolytes can potentially be used in DIBs. GPEs can help reduce the use of solvents and thus lead to a substantial change in the Coulombic efficiency, energy density, and long‐term cycle life of DIBs. Furthermore, GPEs are suited to manufacture compact DIB designs without separators by virtue of their mechanical strength and electrical performance. In this review, we highlight the latest advances in the application of different electrolytes in DIBs, with particular emphasis on GPEs.
A chaotic whale optimization algorithm (CWOA) based multiobjective conjoint spectrum utilization in a cognitive radio (CR) network
Authors: Swathi Bankapalli , Prakash M. Sunil , Krishna Battula Tirumala 
Published: October 2023

SummaryIn this manuscript, a novel energy detection‐based chaotic whale optimization algorithm (ED‐CWOA) is proposed to maximize the spectrum utilization, duration, and threshold in cognitive radio network (CRN). Here, the spectrum utilization is designed taking into account sensing duration and threshold in multi‐objective function is formulated by considering its primary user (PU) protection restrictions and secondary user (SU) throughput. To get the global optimal solution, the CWOA and the multi‐objective optimization problem are finally carried out on a single objective. The execution is performed on NS2 simulator. The simulation outcome demonstrates that the proposed ED‐CWOA system has lower delay 33.878%, 26.206%; higher delivery ratio 35.536%, 22.65%; lower Fairness Index 46.66%, 58.332%; lower energy consumption 25.462%, 35.524%; and higher throughput 96.82%, 49.28% for different nodes analyzed with the existing methods, like energy detection‐based bivariate Levy‐stable bat algorithm (ED‐BLSBA) and genetic algorithm‐based energy detection (ED‐GA), respectively. The simulation outcome indicates that the proposed system may be able to get the optimal global solutions efficiently and accurately.
Brood parasitism and egg recognition in three bunting hosts of the cuckoos
Authors: Zhang Yuhan , Zhong Guo , Wan Guixia , Wang Longwu , Liang Wei 
Published: October 2023

AbstractComparative studies of egg recognition and rejection between various sympatric hosts provide insight into the coevolutionary history of the hosts and parasites, as well as the degree of antagonism between the species. Although buntings are widely considered to be a suitable host taxon for cuckoos, there has been relatively little research on this example of parasitism and host antiparasitic behaviour. Here we provided the first report on brood parasitism and egg recognition in three sympatric ground‐nesting bunting hosts of the common cuckoo (Cuculus canorus), namely the yellow‐throated bunting (Emberiza elegans), south rock bunting (E. yunnanensis), and crested bunting (E. lathami). The results show that for the five breeding seasons during 2018–2022, the parasitism rate by common cuckoos was 0.87% and 0.45% in yellow‐throated buntings and south rock buntings, respectively, whereas the parasitism rate by an unidentified parasite was 4% during 2018–2023 in the crested bunting. The rejection rates of the three bunting hosts for blue non‐mimetic eggs were 89.3%, 88.9%, and 100% for yellow‐throated buntings, south rock buntings, and crested buntings, respectively. The rejection rates for red non‐mimetic eggs by yellow‐throated buntings and south rock buntings were lower at 76.9% and 82.4%, respectively. All three sympatric bunting hosts examined had high levels of egg recognition and egg rejection, suggesting that it may have been subjected to high parasitic history and that egg recognition ability was retained after the loss of parasitism, which needs to be further verified by future experiments.
Chemiluminescence‐driven photoelectrochemical sensor: A mini review
Authors: Luo Yuanjian , Hao Yuanqiang , Zhang Peisheng , Zhang Yintang , Zeng Rongjin , Chen Shu , Xu Maotian 
Published: October 2023

AbstractIn recent years, photoelectrochemical (PEC) sensors have attracted much attention due to their inherent advantages, such as ease of operation, high sensitivity, and structural diversity. Compared with conventional PEC detection methods, self‐illuminated PEC sensors do not require an external light source, which makes them more attractive in terms of instrument miniaturization and simplified operation. Chemiluminescence (CL), a straightforward and efficient excitation source, can be harnessed for PEC sensors. In this review, we provide a summary on the recently reported chemiluminescence‐driven PEC sensors. Initially, we briefly describe the current chemiluminescent systems employed in PEC sensors, including luminol and peroxyoxalate CL systems. Subsequently, the CL‐exciting modes and the design strategies for self‐excited PEC sensors are explored. The subsequent sections of the paper predominantly focus on discussing the applications of such PEC sensors in the detection of various target analytes. Furthermore, future prospects for advancing these PEC sensors are discussed.
Generative and self‐supervised ensemble modeling for multivariate tool wear monitoring
Authors: Joanes Agung' Oroko , James Kimotho , Samuel Kabini , Evan Murimi 
Published: October 2023

AbstractDevelopment of an effective tool wear monitor requires maximum utilization of information from associated data, especially in machine learning based modeling. However, vastly varied annotated training data is required, which is not only expensive but impractical to obtain. In the present work, a contiguous approach of artificial data generation followed up by self‐supervised pre‐training before supervised model fine tuning and final stacked generalized ensembling, has been adopted to develop an effective tool wear monitor in a low data regime. Cross‐validated results of proposed methodology adoption in tool wear prediction on an experimental data set of few labeled samples attained an averaged MAE of 0.035, RMSE of 0.045 and MAPE of on the best case ensemble, which was comparatively superior to a purely supervised‐only trained deep model on the same data set, with an overall accuracy enhancement of over . The proposed approach provides an effective experimental data augmentation technique while simultaneously minimizing aleatoric uncertainty and allowing for utilization of information from often ignored static cutting parameters.
Musicogenic seizures—STXBP1 encephalopathy with reflex seizures
Authors: Desai Nimit N. , Guerrero Carlos , Mesaros Maysen , Schmitt Sarah E. , Campbell Zeke 
Published: October 2023

AbstractMutations in the gene encoding syntaxin binding protein 1 (STXBP1) have been implicated in a wide variety of epileptic encephalopathies. Although the recognized phenotypes of patients with STXBP1 encephalopathies have broadened in recent years, no case of reflex seizures, particularly musicogenic seizures, has been reported in the literature. We present an 18‐year‐old woman with STXBP1 encephalopathy and seizures that are stereotypically reproducible in response to a variety of audible stimuli. An 18‐year‐old woman with a history of profound intellectual disability, confirmed STXBP1 genetic defect via genetic testing, and seizures beginning as infantile spasms during childhood, who presented to the epilepsy monitoring unit for seizure characterization. Her mother reported reproducible seizures triggered by a particular cell phone ringtone, music from a certain automobile television commercial and certain beeping alarm sounds. In response, the patient had clinically stereotyped seizures associated with staring, behavioral arrest, followed by eye deviation to the left, tonic stiffening in upper and lower extremities, and labored breathing lasting between 30 s and 2 min. These seizures were reliably reproducible within a few seconds of exposure to the auditory stimulus. During hospitalization, mother played one of the cell phone ringtones known to trigger seizures for the patient, which resulted in induction of a seizure characterized by diffuse electrodecrement, subsequent emergence of frontal‐predominant theta which was followed by progressive diffuse attenuation and semi‐rhythmic slowing over the right posterior quadrant. This is the first case to describe musicogenic or other reflex seizures in a patient with STXBP1 encephalopathy.
Operation optimization of regional integrated energy systems
Authors: Tang Wentao , Wang Bozhao , Wang Fulin , Tian Yuan , Zou Yunfeng 
Published: October 2023

AbstractTo study the role of energy system demand‐side regulation capacity on carbon emission reduction, we proposed a regional integrated energy system (RIES) optimal dispatch model considering carbon trading cost and dynamic demand response (DR). First, based on the framework of the RIES, the impact of DR of transferable load, reducible load, and substitutable load demand on system operation, considering the response characteristics of electrical and thermal load, was analyzed. Second, the carbon quota of the system was analyzed, and the effect of carbon emissions and carbon capture technology on system operation was considered. Finally, a low carbon optimized operation model of RIES was established with system operating cost and operating efficiency as the target, and system capacity and energy storage power as the constraints. The effectiveness of the optimized operation of the improved intelligent evolutionary algorithm nondominated sorting genetic algorithm II (NSGA‐II) was verified as a research method. The results show that the energy efficiency of the system can be improved by tuning the DR of load demand with the application of the optimal operation model, and a coordination optimization on the economic and low carbon in RIES operation can also be achieved by the improved NSGA‐II algorithm.
Comparative Toxicological Effects of Perfluorooctane Sulfonate and Its Alternative 6:2 Chlorinated Polyfluorinated Ether Sulfonate on Earthworms
Authors: Ge Yanhui , Wang Zhan , Chen Xuelu , Wang Wen , Liu Zhenying , Sun Hongwen , Zhang Lianying 
Published: October 2023

Abstract
High levels of 6:2 chlorinated polyfluorinated ether sulfonate (F-53B), which is a substitute for perfluorooctane sulfonate (PFOS), are detected in various environmental matrices, wildlife, and humans. Chlorinated polyfluorinated ether sulfonate has received increased attention due to its potential risk to ecosystems. However, its toxicity in the soil organisms remains unclear. In the present study, a comparative investigation was conducted on the toxicities of 6:2 Chlorinated polyfluorinated ether sulfonate (F-53B) and PFOS to the earthworm Eisenia. fetida. F-53B was significantly more acutely toxic to earthworms than PFOS, with median lethal concentrations of 1.43 and 1.83 mmol/kg dry soil (~816 and 984 mg/kg dry soil), respectively. Although both F-53B and PFOS, at 0.4 mmol/kg dry soil (=228 and 215 mg/kg dry soil) caused oxidative stress in earthworms, as evidenced by increased superoxide dismutase, peroxidase, and catalase activities as well as malondialdehyde level, the stress caused by F-53B was higher than that caused by PFOS. In transcriptomic and metabolomic studies, negative effects of PFOS and F-53B were observed on several metabolic processes in earthworms, including protein digestion and amino acid absorption, lipid metabolism, and the immune response. Compared with PFOS, F-53B exhibited a weaker disruption of lipid metabolism, comparable potency for toxicity to the immune response, and a stronger potency in extracellular matrix destruction along with apoptosis and ferroptosis induction. Hence, our data suggest that F-53B is more toxic than PFOS to earthworms. The findings provide some new insights into the potential toxicity of F-53B to soil organisms. Environ Toxicol Chem 2024;43:170–181. © 2023 SETAC
The efficacy of remote video‐based behavioral skills training and awareness training on public speaking performance
Authors: Laske Matthew M. , DiGennaro Reed Florence D. , Braren J Turner B. 
Published: October 2023

AbstractThis study evaluated the efficacy of (a) remote video‐based behavioral skills training (BST) with added speech outlines on teaching public speaking behaviors and (b) remote video‐based awareness training (AT) on speech‐disfluency rates. A multiple‐baseline design across speech behaviors was used to evaluate the training. Remote video‐based BST and AT were effective at teaching public speaking behaviors and reducing speech disfluencies, respectively, for both participants. In addition, performance generalized to increased audience size. Although expert ratings of perceived public speaking effectiveness improved following BST, the ratings did not improve and some worsened following AT. Both participants reported satisfaction with video‐based BST and AT. One participant reported greater comfort, confidence, overall ability, and less anxiety as a public speaker following BST. Both participants reported greater improvements in those categories following AT. Our results suggest that public speaking behaviors can be taught using remote video‐based BST and speech disfluencies can be reduced using remote video‐based AT.
Cost‐effectiveness of a decentralized, community‐based “one‐stop‐shop” hepatitis C testing and treatment program in Yangon, Myanmar
Authors: Win Thin Mar , Draper Bridget Louise , Palmer Anna , Htay Hla , Sein Yi Yi , Shilton Sonjelle , Kyi Khin Pyone , Hellard Margaret , Scott Nick 
Published: October 2023

AbstractBackground and AimThe availability of direct‐acting antiviral (DAA) treatment and point‐of‐care diagnostic testing has made hepatitis C (HCV) elimination possible even in low‐ and middle‐income countries (LMICs); however, testing and treatment costs remain a barrier. We estimated the cost and cost‐effectiveness of a decentralized community‐based HCV testing and treatment program (CT2) in Myanmar.MethodsPrimary cost data included the costs of DAAs, investigations, medical supplies and other consumables, staff salaries, equipment, and overheads. A deterministic cohort‐based Markov model was used to estimate the average cost of care, the overall quality‐adjusted life years (QALYs) gained, and the incremental cost‐effectiveness ratio (ICER) of providing testing and DAA treatment compared with a modeled counterfactual scenario of no testing and no treatment.ResultsFrom 30 January to 30 September 2019, 633 patients were enrolled, of whom 535 were HCV RNA‐positive, 489 were treatment eligible, and 488 were treated. Lifetime discounted costs and QALYs of the cohort in the counterfactual no testing and no treatment scenario were estimated to be USD61790 (57 898–66 898) and 6309 (5682–6363) respectively, compared with USD123 248 (122 432–124 101) and 6518 (5894–6671) with the CT2 model of care, giving an ICER of USD294 (192–340) per QALY gained. This “one‐stop‐shop” model of care has a 90% likelihood of being cost‐effective if benchmarked against a willingness to pay of US$300, which is 20% of Myanmar's GDP per capita (2020).ConclusionsThe CT2 model of HCV care is cost‐effective in Myanmar and should be expanded to meet the National Hepatitis Control Program's 2030 target, alongside increasing the affordability and accessibility of services.
Assuring safety of fecal microbiota transplantation in the COVID‐19 era: A single‐center experience
Authors: Chang Tien‐En , Lee Kuei‐Chuan , Lee Pei‐Chang , Wang Yen‐Po , Lin Yi‐Tsung , Huang Hui‐Chun , Luo Jiing‐Chyuan , Ho Hsiang‐Ling , Huang Yi‐Hsiang , Hou Ming‐Chih ,   Task Force for Precision Medicine in Gut Microbial Therapy
Published: October 2023

AbstractBackground and AimFecal microbiota transplantation (FMT) is used to treat recurrent or refractory Clostridioides difficile infection (CDI). In the past, screening of fecal donors required surveillance of personal behavior, medical history, and diseases that could be transmitted by the blood or fecal–oral route. In addition, the exclusion of multidrug‐resistant organisms (MDROs) has been recommended since 2018. This task has become more complicated in the era of the coronavirus disease‐2019 (COVID‐19) pandemic. To prevent fecal transmission of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), it is crucial to commence screening for SARS‐CoV‐2, alongside other traditional tests. Our aim was to investigate whether hidden carriers of SARS‐CoV‐2 were enrolled for stool donation, and the status of the presence or incidence of MDRO during fecal donation in Taiwan.MethodsFecal products collected from March 2019 to December 2022 were tested for MDRO and nucleic acid amplification tests for SARS‐CoV‐2 using the pooling method. The period of fecal product collection crossed the time before and during the COVID pandemic in Taiwan.ResultsA total of 151 fecal samples were collected. The fecal products were tested using polymerase chain reaction (PCR) to detect SARS‐CoV‐2. The results were negative for all stocks. This was similar to the results of MDRO testing. The safety of FMT products has been guaranteed during the pandemic.ConclusionOur FMT center produced MDRO‐free and COVID‐19‐free products before and during the COVID‐19 outbreak in Taiwan. Our protocol was effective for ensuring the safety of FMT products.
Diverse cell death patterns‐related signature for predicting prognosis and drug sensitivity of osteosarcoma patients
Authors: Shi Yanbin , Wu Song , Zhang Xiaolin , Cao Yangbo , Zhang Lina 
Published: October 2023

AbstractBackgroundProgrammed cell death (PCD) is a natural process in which cells undergo controlled self‐destruction, which plays a crucial role in maintaining tissue homeostasis and eliminating damaged or unnecessary cells. The connection between PCD and osteosarcoma was explored in the present study.MethodsTwelve types of PCD were collected for developing a prognostic signature in osteosarcoma using machine learning algorithms. The prognostic value, pathway annotation and drug prediction of the signature were explored.ResultsTelomerase reverse transcriptase (TERT) was found to be a potent hazardous marker in osteosarcoma and could facilitate the proliferation and migration of osteosarcoma.ConclusionsIn summary, the present study has developed a prognostic signature for osteosarcoma and identifies TERT as a potent hazardous gene. The study suggests that further research is needed to address the underlying mechanism of how TERT affects the immune response in osteosarcoma.
On Seymour's and Sullivan's second neighbourhood conjectures
Authors: Ai Jiangdong , Gerke Stefanie , Gutin Gregory , Wang Shujing , Yeo Anders , Zhou Yacong 
Published: October 2023

AbstractFor a vertex of a digraph, (, respectively) is the number of vertices at distance 1 from (to, respectively) and is the number of vertices at distance 2 from . In 1995, Seymour conjectured that for any oriented graph there exists a vertex such that . In 2006, Sullivan conjectured that there exists a vertex in such that . We give a sufficient condition in terms of the number of transitive triangles for an oriented graph to satisfy Sullivan's conjecture. In particular, this implies that Sullivan's conjecture holds for all orientations of planar graphs and triangle‐free graphs. An oriented graph is an oriented split graph if the vertices of can be partitioned into vertex sets and such that is an independent set and induces a tournament. We also show that the two conjectures hold for some families of oriented split graphs, in particular, when induces a regular or an almost regular tournament.