bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endophytic and ectomycorrhizal, an overlooked dual ecological niche? Insights from natural environments andRussulaspecies
Authors: Laurent-Webb Liam , Rech Philippe , Bourceret Amélia , Chaumeton Chloé , Deveau Aurélie , Genola Laurent , Januario Mélanie , Petrolli Rémi , Selosse Marc-André 
Published: January 2024

AbstractEctomycorrhizal (EcM) fungi play key roles in ecosystem functioning, in particular temperate ones. Recent findings suggest that they can endophytically colonize the roots of non-EcM plants. Here we aim at (i) providing new evidence of colonization of non-EcM hosts by EcM fungi, (ii) exploring factors driving such colonization (plant identity, site, root filter), and (iii) providing direct microscopical evidence for endophytism.Using amplicon sequencing (ITS2), we described the root fungal communities of 42 plant species collected at nine locations in France. In two of those sites, we also compared rhizosphere and root fungal communities to identify a potential root filter. Finally, we investigated endophytism inRussulaspp. at twoRussula-rich sites using fluorescencein situhybridization (FISH) paired with confocal microscopy.We find a large but variable share of EcM sequences in roots of non-EcM plant species, in particular nearby EcM hosts, suggesting that endophytism is a secondary ecological niche. Though EcM fungi were more abundant in the rhizosphere compared to roots, their composition was similar to that of roots, suggesting a poor root filter. We observed metabolically active hyphae ofRussulaspp. endophytically colonizing the apoplast of two non-EcM plant species.As shown for other EcM fungi (e.g.,Tuberspp., Ascomycota) we demonstrate the dual EcM/endophyte niche forRussula(Basidiomycota). The ecological consequences of this duality still need to be addressed. The ability to colonize two ecological niches may be a trait kept by EcM fungi which evolved from endophytic fungi, as stipulated by the “waiting room hypothesis”.
High interleukin-3 concentrations prime murine myeloid progenitor cells towards granulocytic differentiation
Authors: Hakobyan Mariam , Rohdjeß Hannah , Lipka Daniel B. 
Published: January 2024

AbstractImpaired differentiation a key feature of many hematopoietic malignancies. To decipher the molecular processes underlying malignant transformation, it is important to understand the mechanisms regulating hematopoietic differentiation. Cell lines derived from transformed hematopoietic progenitors or from leukemia patients have proven to be valuable model systems for mechanistic investigations of hematopoiesis. In the present work, we investigated the cell-intrinsic differentiation capacity of the interleukin 3 (IL-3) dependent murine myeloid progenitor cell line 32D. We demonstrated that 32D cells have a cell-intrinsic granulocytic differentiation potential which requires the presence of high IL-3 concentrations in order to induce granulocytic priming. We also show that 32D cells still proliferate at comparable rates but lack granulocytic priming in the presence of intermediate IL-3 concentrations. This phenotypic plasticity is fully reversible and entirely depends on the IL-3 concentrations present in the culture media. RNA-seq analysis revealed that, among other myeloid genes, the expression ofCsf3ris strongly induced exclusively in the presence of high IL-3 concentrations, likely explaining the granulocytic differentiation in response to G-CSF treatment. Together, our work provides detailed cellular and molecular insights into the phenotypic plasticity of 32D cells driven by different IL-3 concentrations and suggest culture conditions for different experimental set ups.Key points32D cells represent a murine myeloid cell line with endogenous granulocytic differentiation potential.32D cells present molecular and functional plasticity depending on their culture conditions.High IL-3 culture conditions prime 32D cells towards the granulocytic lineage.Low IL-3 culture conditions lead to a reversible loss of the granulocytic differentiation potential of 32D cells while fully maintaining their proliferative activity.
ImprintedGrb10, encoding growth factor receptor bound protein 10, regulates fetal growth independently of the insulin-like growth factor type 1 receptor (Igf1r) and insulin receptor (Insr) genes
Authors: Moorwood Kim , Smith Florentia M. , Garfield Alastair S. , Ward Andrew 
Published: January 2024

AbstractBackgroundOptimal size at birth dictates perinatal survival and long-term risk of developing common disorders such as obesity, type 2 diabetes and cardiovascular disease. The imprintedGrb10gene encodes a signalling adaptor protein capable of inhibiting receptor tyrosine kinases, including the insulin receptor (Insr) and insulin-like growth factor type 1 receptor (Igf1r).Grb10restricts fetal growth such thatGrb10knockout (KO) mice are at birth some 25-35% larger than wild type. Using a mouse genetic approach, we test the widely held assumption that Grb10 influences growth through interaction with Igf1r, which has a highly conserved growth promoting role.ResultsShould Grb10 interact with Igf1r to regulate growthGrb10:Igf1rdouble mutant mice should be indistinguishable fromIgf1rKO single mutants, which are around half normal size at birth. Instead,Grb10:Igf1rdouble mutants were intermediate in size betweenGrb10KO andIgf1rKO single mutants, indicating additive effects of the two signalling proteins having opposite actions in separate pathways. Some organs examined followed a similar pattern, thoughGrb10KO neonates exhibited sparing of the brain and kidneys, whereas the influence ofIgf1rextended to all organs. An interaction between Grb10 and Insr was similarly investigated. While there was no general evidence for a major interaction for fetal growth regulation, the liver was an exception. The liver inGrb10KO mutants was disproportionately overgrown with evidence of excess lipid storage in hepatocytes, whereasGrb10:Insrdouble mutants were indistinguishable fromInsrsingle mutants or wild types.ConclusionsGrb10 acts largely independently of Igf1r or Insr to control fetal growth and has a more variable influence on individual organs. Only the disproportionate overgrowth and excess lipid storage seen in theGrb10KO neonatal liver can be explained through an interaction between Grb10 and the Insr. Our findings are important for understanding how positive and negative influences on fetal growth dictate size and tissue proportions at birth.
Nr4a2 blocks oAβ-mediated synaptic plasticity dysfunction and ameliorates spatial memory deficits in the APPSw,Indmouse
Authors: Català-Solsona Judit , Lutzu Stefano , Lituma Pablo J. , Fábregas-Ordoñez Cristina , Siedlecki Dolores , Giménez-Llort Lydia , Miñano-Molina Alfredo J. , Saura Carlos A. , Castillo Pablo E. , Rodriguez-Álvarez José 
Published: January 2024

ABSTRACTAlzheimer’s disease AD is associated with disruptions in neuronal communication, especially in brain regions crucial for learning and memory, such as the hippocampus. The amyloid hypothesis suggests that the accumulation of amyloid-beta oligomers (oAβ) contributes to synaptic dysfunction by internalisation of synaptic AMPA receptors. Recently, it has been reported that Nr4a2, a member of the Nr4a family of orphan nuclear receptors, plays a role in hippocampal synaptic plasticity by regulating BDNF and synaptic AMPA receptors. Here, we demonstrate that oAβ inhibits activity-dependent Nr4a2 activation in hippocampal neurons, indicating a potential link between oAβ and Nr4a2 down-regulation. Furthermore, we have observed a reduction in Nr4a2 protein levels in postmortem hippocampal tissue samples from early AD stages. Pharmacological activation of Nr4a2 proves effective in preventing oAβ-mediated synaptic depression in the hippocampus. Notably, Nr4a2 overexpression in the hippocampus of AD mouse models ameliorates spatial learning and memory deficits. In conclusion, the findings suggest that oAβ may contribute to early cognitive impairment in AD by blocking Nr4a2 activation, leading to synaptic dysfunction. Thus, our results further support that Nr4a2 activation is a potential therapeutic target to mitigate oAβ-induced synaptic and cognitive impairments in the early stages of Alzheimer’s disease.
High-Sensitivity ac-Charge Detection with a MHz-Frequency Fluxonium Qubit
Authors: Najera-Santos B.-L. , Rousseau R. , Gerashchenko K. , Patange H. , Riva A. , Villiers M. , Briant T. , Cohadon P.-F. , Heidmann A. , Palomo J. , Rosticher M. , le Sueur H. , Sarlette A. , Smith W. C. , Leghtas Z. , Flurin E. , Jacqmin T. , Deléglise S. 
Published: January 2024

Owing to their strong dipole moment and long coherence times, superconducting qubits have demonstrated remarkable success in hybrid quantum circuits. However, most qubit architectures are limited to the GHz frequency range, severely constraining the class of systems they can interact with. The fluxonium qubit, on the other hand, can be biased to very low frequency while being manipulated and read out with standard microwave techniques. Here, we design and operate a heavy fluxonium with an unprecedentedly low transition frequency of 1.8 MHz. We demonstrate resolved sideband cooling of the “hot” qubit transition with a final ground state population of 97.7%, corresponding to an effective temperature of 23  μK. We further demonstrate coherent manipulation with coherence times T1=34  μs, T2*=39  μs, and single-shot readout of the qubit state. Importantly, by directly addressing the qubit transition with a capacitively coupled waveguide, we showcase its high sensitivity to a radio-frequency field. Through cyclic qubit preparation and interrogation, we transform this low-frequency fluxonium qubit into a frequency-resolved charge sensor. This method results in a charge sensitivity of 33  μe/Hz, or an energy sensitivity (in joules per hertz) of 2.8ℏ. This method rivals state-of-the-art transport-based devices, while maintaining inherent insensitivity to dc-charge noise. The high charge sensitivity combined with large capacitive shunt unlocks new avenues for exploring quantum phenomena in the 1–10 MHz range, such as the strong-coupling regime with a resonant macroscopic mechanical resonator.

Published by the American Physical Society
2024
The retail environment design (RED) scale: conceptualization and measurement
Authors: Steen Julie , Rutherford Brian N. , Babin Barry J. , Hair, Jr. Joseph F. 
Published: January 2024

Purpose
Design is an important construct in the retail environment literature. Yet, the measures used for design have not followed appropriate scale development procedures. The purpose of this study is to provide a conceptual definition and then develop a scale for retail environment design (RED).

Design/methodology/approach
Interviews with both consumers and marketing researchers are used to generate a potential list of items. Using four different studies, these items are refined, and the RED scale is offered.

Findings
This study develops and validates the four-dimensional RED scale to measure the design of retail environments. The dimensions are functional, aesthetic, lighting and signage.

Research limitations/implications
The newly developed RED scale will allow retailing researchers to measure lighting and signage qualities as part of retail design, measure design of retail environments more accurately and allow different studies to be compared.

Practical implications
The newly developed RED scale will allow retailers to better understand customers’ perceptions of the four dimensions of design. Retailers spend significant time and money designing and redesigning retail environments. The RED scale will enable managers to ensure these significant investments create competitive advantages and an appropriate return on investment.

Originality/value
A scale to measure retail environment design is developed. The scale includes two dimensions (lighting and signage) that are not typically investigated.
Nexus between formal institutions and inward FDI in India: a nonlinear autoregressive distributive lag approach
Authors: Patel Richa , Mohapatra Dipti Ranjan , Yadav Sunil Kumar 
Published: January 2024

PurposeThis study presents time-series data estimations on the association between the indicators of institutional environment and inward foreign direct investment (FDI) in India utilizing a comprehensive data set from 1996 to 2021.Design/methodology/approachThe study employs the nonlinear autoregressive distributive lag (NARDL) model. The asymmetric ARDL framework evaluates the existence of cointegration among the factors under study and highlights the underlying nonlinear effects that may exist in the long and short run.FindingsThe significance of coefficients of negative shock to “control of corruption” and positive shock to “rule of law” is greater when compared to “government effectiveness, regulatory quality, political stability/absence of violence.” The empirical outcomes suggest the positive influence of rule of law, political stability and government effectiveness on FDI inflows. A high “regulatory quality” is observed to deter foreign investment. The “voice and accountability” index and negative shocks to the “rule of law” are exhibited to have no substantial impact on the amount of FDI that the country receives.Originality/valueThis study empirically examines the institutional determinants of FDI in India for a comprehensive period of 1996–2021. The study's findings imply that quality of the institutional environment has a significant bearing on India's inward FDI.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-05-2023-0375
DoS attack detection using Aquila deer hunting optimization enabled deep belief network
Authors: Thomas Merly , B.B. Meshram 
Published: January 2024

Purpose
Denial-of-service (DoS) attacks develop unauthorized entry to various network services and user information by building traffic that creates multiple requests simultaneously making the system unavailable to users. Protection of internet services requires effective DoS attack detection to keep an eye on traffic passing across protected networks, freeing the protected internet servers from surveillance threats and ensuring they can focus on offering high-quality services with the fewest response times possible.

Design/methodology/approach
This paper aims to develop a hybrid optimization-based deep learning model to precisely detect DoS attacks.

Findings
The designed Aquila deer hunting optimization-enabled deep belief network technique achieved improved performance with an accuracy of 92.8%, a true positive rate of 92.8% and a true negative rate of 93.6.

Originality/value
The introduced detection approach effectively detects DoS attacks available on the internet.
Striking gold: navigating the education massification maze for work readiness
Authors: Enstroem Rickard , Schmaltz Rodney 
Published: January 2024

PurposeThis study investigates the impact of large-scale teaching in higher education on students’ preparedness for the workforce within the context of evolving labour market demands, the expansion of higher education and the application of high-impact teaching strategies. It synthesizes perspectives on employer work readiness, the challenges and opportunities of large-scale teaching and strategies for fostering a dynamic academia-industry feedback loop. This multifaceted approach ensures the relevance of curricula and graduates’ preparedness while addressing the skills gap through practical recommendations for aligning teaching methodologies with employer expectations.Design/methodology/approachThe research methodically examines the multifaceted challenges and opportunities inherent in large-scale teaching. It focuses on sustaining student engagement, maintaining educational quality, personalizing learning experiences and cultivating essential soft skills in extensive student cohorts.FindingsThis study highlights the critical role of transversal skills in work readiness. It also uncovers that despite its challenges, large-scale teaching presents unique opportunities. The diversity of large student groups mirrors modern workplace complexities, and technological tools aid in personalizing learning experiences. Approaches like peer networking, innovative teaching methods, real-world simulations and collaborative resource utilization enrich education. The importance of experiential learning for augmenting large-scale teaching in honing soft skills is emphasized.Originality/valueThis manuscript contributes to the discourse on large-scale teaching, aligning it with employer expectations and the dynamic requirements of the job market. It offers a nuanced perspective on the challenges and opportunities this educational approach presents, providing insights for crafting engaging and effective learning experiences in large cohorts. The study uniquely integrates experiential learning, co-creation in education and industry-academia feedback loops, underscoring their importance in enhancing student work readiness in large-scale teaching.
Seed dispersal by frugivores without seed swallowing: Evaluating the contributions of stomatochoric seed dispersers
Authors: McConkey Kim R. , Sushma H. S. , Sengupta Asmita 
Published: January 2024

Abstract

The process of seed dispersal that underpins ecosystem maintenance is performed by diverse arrays of fruit‐eating animals. However, seed dispersal studies are primarily focused on a subset of these animal communities that disperse seeds by endozoochory. Stomatochory (seed dispersal in which seeds are carried externally and are not swallowed) is rarely considered to be effective, despite an increasing number of taxa‐focused studies that indicate otherwise.

We collated the available information on stomatochory to provide a quantitative overview of the dispersal mechanism, including plant types and fruit traits dispersed, dispersal distances and germination potential for all available taxa. We compared seed sizes dispersed, dispersal distances and germination potential with corresponding data on endozoochory for bats and primates. We also identified the main taxa dispersing seeds by stomatochory and assessed what factors influenced the distances that they carried seeds to.

Stomatochoric dispersers can displace large quantities of seeds, including large seeds and those of large fruits, and over short to long distances (>1 km). Compared with similar‐sized endozoochoric dispersers, they can disperse larger seeds, but over shorter distances, on average. Similar to endozoochory, seed handling by stomatochory also improves the germination potential of dispersed seeds. Dispersal distances achieved by stomatochory were influenced by body mass, daily path length, seed width, fruit type and seed handling techniques.

Five main taxonomic groups of stomatochoric dispersers were identified: bats, parrots, squirrels, corvids and Old World monkeys (cercopithecines). Parrots perform dispersal services for the largest fruits and over the longest distances. However, given the lack of research on stomatochory, it is likely other taxa are also important stomatochoric dispersers but have not been identified yet.

More research attention must be directed towards seed dispersal services that are not provided by endozoochory. Many stomatochoric dispersers are common animals within communities and could be playing dominant seed dispersal roles, even without swallowing seeds. Community‐wide studies should incorporate all seed dispersal interactions, rather than focusing solely on endozoochory. This will ensure a more robust understanding of community‐wide patterns.

Read the free Plain Language Summary for this article on the Journal blog.
The exometabolome of microbial communities inhabiting bare ice surfaces on the southern Greenland Ice Sheet
Authors: Doting Eva L. , Jensen Marie B. , Peter Elisa K. , Ellegaard‐Jensen Lea , Tranter Martyn , Benning Liane G. , Hansen Martin , Anesio Alexandre M. 
Published: January 2024

AbstractMicrobial blooms colonize the Greenland Ice Sheet bare ice surface during the ablation season and significantly reduce its albedo. On the ice surface, microbes are exposed to high levels of irradiance, freeze–thaw cycles, and low nutrient concentrations. It is well known that microorganisms secrete metabolites to maintain homeostasis, communicate with other microorganisms, and defend themselves. Yet, the exometabolome of supraglacial microbial blooms, dominated by the pigmented glacier ice algae Ancylonema alaskanum and Ancylonema nordenskiöldii, remains thus far unstudied. Here, we use a high‐resolution mass spectrometry‐based untargeted metabolomics workflow to identify metabolites in the exometabolome of microbial blooms on the surface of the southern tip of the Greenland Ice Sheet. Samples were collected every 6 h across two diurnal cycles at 5 replicate sampling sites with high similarity in community composition, in terms of orders and phyla present. Time of sampling explained 46% (permutational multivariate analysis of variance [PERMANOVA], pseudo‐F = 3.7771, p = 0.001) and 27% (PERMANOVA, pseudo‐F = 1.8705, p = 0.001) of variance in the exometabolome across the two diurnal cycles. Annotated metabolites included riboflavin, lumichrome, tryptophan, and azelaic acid, all of which have demonstrated roles in microbe–microbe interactions in other ecosystems and should be tested for potential roles in the development of microbial blooms on bare ice surfaces.
Willingness to pay for improved public education and public healthcare systems: the role of income mobility prospects
Authors: Wiese Rasmus , Eriksen Steffen 
Published: January 2024

AbstractIncome mobility prospects affect individuals’ willingness to pay higher taxes, or give part of their income, to improve the public healthcare and public education systems. In line with the prospects of the upward mobility hypothesis, risk‐willing individuals who expect to move far up the socio‐economic ladder are less willing to pay compared with individuals who expect no upward transition. Consistent with a social insurance effect, risk‐averse individuals who hold modest upward prospects are more willing to pay compared with individuals without upward prospects. These findings are based on more than 19,000 observations from the third round of the Life in Transition Survey.