bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymorphism of the Forkhead box-O3 (FOXO3) Longevity Gene rs2802292 and senescence-associated secretory phenotype (SASP) in Indonesian Elderly Population
Authors: Hardiany Novi Silvia , Nurfiyana Wahyu , Iswanti Febriana Catur 
Published: February 2024

BACKGROUND: Forkhead box O3 (FOXO3) is a transcription factor that regulates stress resistance, metabolism, cell cycle, and apoptosis. Several studies exhibited the association of the FOXO3 polymorphism rs2802292 with human longevity and protects individuals from degenerative diseases. The emergence of degenerative diseases in the elderly is associated with the accumulation of senescent cells that secrete a secretome known as a senescence-associated secretory phenotype (SASP) consists of several cytokines, chemokines, growth factors, proteases. OBJECTIVE: The aim of this research was to analysis polymorphism of FOXO3 gene rs2802292 G-allele and its impact to the SASP by measuring IL-1α, IL-6, IL-8, and IL-10 in Indonesian elderly populations. METHODS: This study was conducted on 92 elderly subjects living at Jakarta. DNA was isolated from the whole blood then continued with PCR and sequencing for FOXO3 rs2802292 analysis. SASP was analyzed from the plasma using Luminex. Statistical analysis was performed using ANOVA and Kruskal Wallis. RESULTS: The results showed that FOXO3 rs2802292 was detected in Indonesian elderly population as follows GG, GT, TT genotype frequencies were 17.4 %, 42.4 % and 40.2 % respectively. Meanwhile, G and T allele frequencies based on the Hardy-Weinberg equilibrium test were 0.385 and 0.615 respectively. GG genotype was significantly found in subjects with longevity. Nevertheless, SASP analysis was found no significant differences both among GG, GT, TT genotypes. CONCLUSION: The frequency of FOXO3 rs2802292 G-allele in Indonesian elderly population was 0.385 and significantly detected in subjects with longevity. However, the FOXO3 rs2802292 polymorphism did not affect cytokines level as the component of SASP.
Aktualisasi Financial Technology Syariah Dalam Perspektif Maqasid Syariah Pada Era Digital
Authors: Kurrohman Taufik , Harapan Aria Dimas 
Published: February 2024

Dewasa ini financial techcnologi berkembang begitu pesat dan menjadi alternatif bagi masyarakat yang menginginkan kecepatan pelayanan di sisi lain financial techcnologi syariah juga kini menjadi pilihan bagi masyarakat muslim, namun pelaksanaannya apakah sudah memenuhi kaidah-kaidah berdasarkan prinsip-prinsip syariah. Tujuan penelitian ini adalah mengetahui aktualisasi Financial Technology Syariah yang sesuai dengan maqasid syariah dan mengetahui bagaimana hambatan-hambatan yang dihadapi di dalam melaksanakan transaksi Financial Technology Syariah. Rumusan dalam penelitian ini yaitu Pertama, Bagaimanakah aktualisasi Financial Technology Syariah yang sesuai dengan maqasid syariah Kedua, Bagaimana hambatan-hambatan yang dihadapi di dalam melaksanakan transaksi Financial Technology Syariah. Metode penelitian yang gunakan dalam penelitian ini adalah penelitian normatif yang dikuatkan dengan data emperis. Hasil penelitian menunjukan aktualisasi Financial Technology Syariah belum sesuai dengan maqasid syariah dalam tatanan penyajian akad dan sistem yang digunakan, Adapun hambatan yang dihadapi dalam Financial Technology Syariah adalah pelaksanaan tidak melalui proses akad secara tatap muka, nasabah tidak begitu memahami karena tidak ada penjelasan secara lisan dan yang ketiga sistem yang digunakan relatif sama dengan sistem yang digunakan konvensional.
KECEMASAN PADA WANITA YANG MENGALAMI INFERTILITAS
Authors: Susanto Bela Novita Amaris , Megananda Nadya Karlina , Yulianto Sigit 
Published: February 2024

Infertilitas yang terjadi pada wanita adalah faktor utama penyebab pasangan untuk memiliki keturunan. Permasalahan infertilitas terdapat pada sistem reproduksi wanita. Kondisi ini diyakini dapat menyebabkan gangguan kecemasan dan kesulitan dalam menghadapi masalah infertilitas. Penelitian ini bertujuan untuk mengetahui gambaran kecemasan yang dialami wanita dengan infertilitas. Penelitian riset kualitatif dengan pendekatan phenomenology dengan jumlah responden 5 orang anggota komunitas bayi tabung Indonesia yang dilakukan secara daring melalui zoom meeting dengan teknik wawancara. Penelitian dilakukan pada Mei-Agustus 2022. Proses analisa data menggunakan tahapan antara lain pengumpulan data, reduksi data, penyajian data dan penarikan kesimpulan. Hasil dari penelitian ini menunjukkan bahwa terjadi kecemasan pada wanita dengan infertilitas yang digambarkan melalui adanya penyebab, respon, dimensi dan dampak dari kecemasan. Diharapkan wanita dengan kondisi infertilitas dapat menentukan strategi koping adaptif yang diterapkan pada diri sendiri.
LAPORAN KASUS PENERAPAN INTERVENSI TEKNIK RELAKSASI GENGGAM JARI UNTUK MENGATASI NYERI AKUT PADA PASIEN POST OPERASI GIANT CELL TUMOR (GCT)
Authors: Andini Melina Rike , Nugraha Bambang Aditya , Purba Chandra Isabella Hostanida 
Published: February 2024

Giant Cell Tumor adalah salah satu tumor tulang jinak yang memiliki pertumbuhan lokal yang agresif untuk bermetastasis. Penatalaksanaan tumor ini dapat dilakukan dengan tindakan operasi untuk mengangkat masa dan mencegah tumor menyebar. Keluhan yang dirasakan setelah tindakan pembedahan adalah nyeri post operasi karena adanya kerusakan jaringan. Manajemen nyeri dapat dilakukan dengan farmakologi dan non farmakologi. Intervensi non farmakologi yang diberikan adalah teknik distraksi dengan relaksasi genggam jari. Penelitian ini bertujuan untuk menggambarkan pemberian terapi relaksasi genggam jari pada pasien dengan nyeri akut post operasi Giant Cell Tumor. Metode pada penelitian ini adalah studi kasus dengan memberikan asuhan keperawatan pada pasien. Pengumpulan data yaitu dengan wawancara dan observasi. Subjek pada penelitian ini adalah satu orang pasien post operasi Giant Cell Tumor yang mempunyai masalah keperawatan nyeri akut. Pada studi kasus ini hasil menunjukkan sebelum diberikan intervensi teknik genggam jari skala nyeri yang dirasakan klien 6 (0-10) dan setelah diberikan intervensi terdapat penurunan skala nyeri menjadi 2 (0-10). Teknik distraksi dengan cara relaksasi genggam jari yang diberikan selama 20 menit perhari selama 3 hari pada pasien post operasi Giant Cell Tumor mampu menurunkan intensitas skala nyeri yang dirasakan klien dan memberikan efek rileks, tenang dan nyaman. Teknik relaksasi genggam jari ini dapat dijadikan sebagai intervensi pendamping selain pemberian obat analgetik pada pasien dengan keluhan nyeri akut post operasi Giant Cell Tumor di Rumah Sakit.
FULLER VS. BAKER: MUSAS DE LA REPRESENTACIÓN ARTÍSTICA DEL MOVIMIENTO EN LOS ALBORES DEL SIGLO XX
Authors: Amado Lorenzo Antonio , Arévalo Rodríguez Federico , Escoda Pastor María Carmen 
Published: February 2024

A finales del XIX, la bailarina americana Loïe Fuller se convirtió en una musa de las vanguardias parisinas. Aunque treinta años después, el contexto había cambiado radicalmente, un fenómeno similar se repitió con su compatriota Josephine Baker. Los artistas decó, simbolistas, futuristas y primeros cineastas materializaron las evoluciones de ambas sobre los escenarios. Ellas no solo suscitaron el interés del público de todas las capas sociales, sino el de importantes intelectuales y artistas. La investigación ha implicado la revisión crítica de estudios específicos sobre ambas: autobiografías, hemerotecas de prensa o trabajos recientes que reivindican la figura innovadora de Fuller, así como la evolución de la danza y su relación con otras artes. Se analiza en paralelo el impacto que estas dos musas contemporáneas provocaron en disciplinas artísticas como la pintura, escultura, ilustración, cine o arquitectura, prestando especial atención a cómo la representación del movimiento se materializó en cada una de ellas. A partir de esta revisión, se constatan coincidencias, paralelismos y divergencias que ponen de manifiesto que el auténtico vínculo común y atracción real para los artistas no fueron precisamente las dos bailarinas y su propio arte, sino sus revolucionarias, novedosas y sugestivas interpretaciones dinámicas sobre los escenarios: sutiles y elegantes en Fuller, vertiginosas y electrizantes en Baker. El impacto paralelo de ambas en los artistas de sus épocas es el tema vertebrador de la investigación. Más allá de su conocido rol como artistas de espectáculos y estimuladoras del arte de vanguardia, personificaron algo no valorado entonces en su justa medida, debido a una falta de perspectiva temporal. Representaron, sin saberlo, un punto de inflexión hacia una concepción moderna de las artes que rompía con el clasicismo de modelos artísticos estáticos para evolucionar hacia un interés creciente por fenómenos mucho más dinámicos.
Explorando la Arquitectura del Paisaje contemporánea: 12.º Premio Internacional Rosa Barba Casanovas
Authors: Cervera Alonso de Medina Marina 
Published: February 2024

La 12.ª edición de la Bienal de Paisaje de Barcelona concluyó el 28 de noviembre, destacando la arquitectura del paisaje a través del prestigioso Premio Internacional de Arquitectura del Paisaje Rosa Barba Casanovas. Bajo el lema "La Poética de la Remediación", el evento celebró las mejores prácticas de profesionales y docentes en universidades especializadas.
El jurado internacional, liderado por Gareth Doherty de la Universidad de Harvard, seleccionó 11 proyectos finalistas entre 225 nominaciones. Estos proyectos, ubicados en diversas regiones del mundo, abordaron desafíos sociales y ambientales, destacando la adaptación al cambio climático, la regeneración de espacios urbanos y la co-creación con comunidades locales.
El proyecto ganador, el "Parque de la Cantera de Tangshan" en China, de Z&T Studio, recibió elogios por su excelencia en diseño, impacto social y narrativa excepcional. Además, tres proyectos recibieron menciones honoríficas por su contribución excepcional al campo de la arquitectura del paisaje.
Los proyectos finalistas compartieron patrones comunes, como el enfoque en el impacto social positivo, la adaptación al cambio climático y la atención al contexto local y regional. La importancia de la co-creación y la integración de soluciones basadas en la naturaleza también se destacó.
La ceremonia incluyó menciones honoríficas para proyectos en Medellín, Sudáfrica y el Reino Unido. El gran ganador, el Parque de la Cantera de Tangshan, se destacó como un modelo ejemplar que aborda los desafíos contemporáneos de manera integral.
El evento también reconoció el Premio de Escuelas de Paisaje Ribas Piera, otorgado a la Universidad UiT Arctic en Noruega, destacando la investigación basada en el sitio y la ética en la creación de entornos sostenibles.
La conclusión subrayó la importancia continuada de la arquitectura del paisaje en la creación de entornos sostenibles y significativos en medio de los desafíos actuales y futuros, desde la crisis climática hasta la rápida urbanización.
MARTin—an open-source platform for microarray analysis
Authors: Kreissner Kai O. , Faller Benjamin , Talucci Ivan , Maric Hans M. 
Published: February 2024

Background: Microarray technology has brought significant advancements to high-throughput analysis, particularly in the comprehensive study of biomolecular interactions involving proteins, peptides, and antibodies, as well as in the fields of gene expression and genotyping. With the ever-increasing volume and intricacy of microarray data, an accurate, reliable and reproducible analysis is essential. Furthermore, there is a high level of variation in the format of microarrays. This not only holds true between different sample types but is also due to differences in the hardware used during the production of the arrays, as well as the personal preferences of the individual users. Therefore, there is a need for transparent, broadly applicable and user-friendly image quantification techniques to extract meaningful information from these complex datasets, while also addressing the challenges posed by specific microarray and imager formats, which can flaw analysis and interpretation.Results: Here we introduce MicroArray Rastering Tool (MARTin), as a versatile tool developed primarily for the analysis of protein and peptide microarrays. Our software provides state-of-the-art methodologies, offering researchers a comprehensive tool for microarray image quantification. MARTin is independent of the microarray platform used and supports various configurations including high-density formats and printed arrays with significant x and y offsets. This is made possible by granting the user the ability to freely customize parts of the application to their specific microarray format. Thanks to built-in features like adaptive filtering and autofit, measurements can be done very efficiently and are highly reproducible. Furthermore, our tool integrates metadata management and integrity check features, providing a straightforward quality control method, along with a ready-to-use interface for in-depth data analysis. This not only promotes good scientific practice in the field of microarray analysis but also enhances the ability to explore and examine the generated data.Conclusion: MARTin has been developed to empower its users with a reliable, efficient, and intuitive tool for peptidomic and proteomic array analysis, thereby facilitating data-driven discovery across disciplines. Our software is an open-source project freely available via the GNU Affero General Public License licence on GitHub.
Left ventricular to left arial volume ratio in the assessment of filling pressure in patients with dyspnoea and preserved ejection fraction
Authors: Palka Przemysław , Hilling-Smith Roland , Swann Rohan , Allwood Sean , Moore Alexander , Bian Chris , Lange Aleksandra 
Published: February 2024

IntroductionAssessing filling pressure (FP) remains a clinical challenge despite advancements in non-invasive imaging techniques. This study investigates the utility of echocardiographic left ventricular (LV) to left atrial (LA) volume ratio in estimating the resting FP in patients with dyspnoea and preserved ejection fraction (EF).MethodsThis study is a prospective, single-centre analysis of 53 consecutive patients with dyspnoea (New York Heart Association grade 2 or 3) and LVEF of ≥50% (mean age 71 ± 10 years) who underwent cardiac catheterisation, including direct measurement of LA pressure at rest using retrograde technique. Echocardiographic data were obtained 1.5 ± 1.0 h after cardiac catheterisation. The patients were divided into two groups: Group 1 consisted of individuals with elevated FP, indicated by a mean LA pressure or mean pulmonary capillary wedge pressure of &gt;12 mmHg, and Group 2 comprised of patients with normal FP. The LV and LA volumes were measured at three specific points: the minimum volume (LVES, LAmin), the volume during diastasis (LVdias, LAdias), and the maximum volume (LVED, LAmax). The corresponding LV/LA volume ratios were analysed: end-systole (LVES/LAmax), diastasis (LVdias/LAdias), and end-diastole (LVED/LAmin).ResultsThe patients in Group 1 exhibited lower LV/LA volume ratios compared with those in Group 2 (LVES/LAmax 0.44 ± 0.12 vs. 0.60 ± 0.23, P = 0.0032; LVdias/LAdias 1.13 ± 0.30 vs. 1.56 ± 0.49, P = 0.0007; LVED/LAmin 2.71 ± 1.57 vs. 4.44 ± 1.70, P = 0.0004). The LV/LA volume ratios correlated inversely with an increased FP (LVES/LAmax, r = −0.40, P = 0.0033; LVdias/LAdias, r = −0.45, P = 0.0007; LVED/LAmin, r = −0.55, P &lt; 0.0001). Among all the measurements, the LVdias/LAdias ratio demonstrated the highest discriminatory power to distinguish patients with elevated FP from normal FP, with a cut-off value of ≤1.24 [area under the curve (AUC) = 0.822] for the entire group, encompassing both sinus rhythm and atrial fibrillation. For patients in sinus rhythm specifically, the cut-off value was ≤1.28 (AUC = 0.799), with P &lt; 0.0001 for both. The LVdias/LAdias index demonstrated non-inferiority to the E/e' ratio [ΔAUC = 0.159, confidence interval (CI) = −0.020–0.338; P = 0.0809], while surpassing the indices of LA reservoir function (ΔAUC = 0.249, CI = 0.044–0.454; P = 0.0176), LA reservoir strain (ΔAUC = 0.333, CI = 0.149–0.517; P = 0.0004), and LAmax index (ΔAUC = 0.224, CI = 0.043–0.406; P = 0.0152) in diagnosing patients with elevated FP.ConclusionThe study presents a straightforward and reproducible method for non-invasive estimation of FP using routine TTE in patients with dyspnoea and preserved EF. The LVdias/LAdias index emerges as a promising indicator for identifying elevated FP, demonstrating comparable or even superior performance to established parameters.
Fasting and meal-related zonulin serum levels in a large cohort of obese children and adolescents
Authors: Pepe Giorgia , Corica Domenico , Currò Monica , Aversa Tommaso , Alibrandi Angela , Ientile Riccardo , Caccamo Daniela , Wasniewska Malgorzata 
Published: February 2024

IntroductionZonulin recently emerged as a valuable biological marker to assess the integrity of the intestinal mucosal barrier. Nevertheless, data about zonulin in pediatric age are extremely scarce. Aim of this study was to investigate the relationship between serum zonulin levels, both fasting and postprandial, with body mass index (BMI) and biochemical markers of insulin resistance (IR), insulin sensitivity, b-cell function and cardio-metabolic risk in obese non-diabetic youths.MethodsOne hundred and four children and adolescents with obesity (BMI ≥ 2.0 SDS) were enrolled (mean age 11.43 ± 2.66). All the patients underwent clinical and biochemical assessment, including oral glucose tolerance test (OGTT) and liver ultrasonography. Zonulin serum levels were measured at fasting state, at 60-minute and 120-minute OGTT timepoint.ResultsImpaired fasting glycaemia and impaired glucose tolerance were documented in 27.9% and 11.5% of patients, respectively. IR was documented in 69.2% of cases. Liver steatosis was diagnosed in 39.4%. Zonulin serum levels significantly increased from baseline to 60-minute and 120-minute OGTT timepoint (p positive correlation between BMI SDS and serum zonulin levels at 120-minute OGTT timepoint (p highlighted a positive association of zonulin fasting levels with IR and glutamicoxalacetic transaminase levels (GOT, p zonulin levels were demonstrated for age, sex, pubertal status, glucose, lipid profile and the other obesity-related parameters.DiscussionOur results show, for the first time in a pediatric cohort, the meal-related pattern of secretion of serum zonulin, which tends to significantly increase during and at 2-hours postprandial assessment. Even if the underlying mechanisms associating intestinal permeability and obesity have not been fully elucidated yet, our data confirm a close relationship between zonulin concentration and obesity in pediatric population. IR seems to significantly influence zonulin serum levels, thus a central role of IR in this pathway is conceivable.
Triglyceride–glucose index change and chronic kidney disease progression in a Chinese hypertensive population
Authors: Yu Chao , Shi Yumeng , Wang Tao , Zhu Lingjuan , Zhou Wei , Bao Huihui , Cheng Xiaoshu 
Published: February 2024

BackgroundThe impact of triglyceride–glucose (TyG) index variations on chronic kidney disease (CKD) progression remains unexplored. To investigate the effects of the TyG index and its dynamic changes on CKD progression.MethodThis prospective cohort study included data from 8,418 hypertensive participants. The exposure variable in this study was defined as the difference between the TyG index at the last visit from that at baseline. The study’s outcome variable was the progression of CKD, defined as follows: for subjects with an estimated glomerular filtration rate (eGFR) ≥60 mL/min, a ≥30% decrease in eGFR with a final follow-up value &lt;60 mL/min; for those with an eGFR &lt;60 mL/min, a ≥50% decrease in eGFR; or terminal renal failure requiring dialysis.ResultsDuring a median follow-up period of 48 months, 1077 patients were diagnosed with CKD progression. In the fully adjusted Model 3, patients with a change in the TyG index &lt;0 exhibited a significantly decreased 13% risk of CKD progression (HR: 0.87, 95% CI: 0.76–0.98) compared to those with a change in the TyG index≥0 group. Subgroup analyses showed that changes in the TyG index significantly increased the risk of CKD progression only in patients with diastolic blood pressure (DBP) &lt;90mmHg. In the path analysis, baseline TyG was associated with follow-up eGFR (the standard regression coefficient was 1.26 [95% CI, 0.45–2.06]).ConclusionsOur findings suggest that TyG variability may serve as a useful tool for identifying individuals at risk of CKD progression, particularly hypertensive patients with normal DBP levels.
Prognostic stratification of sepsis through DNA damage response based RiskScore system: insights from single-cell RNA-sequencing and transcriptomic profiling
Authors: Lin Qingjiang , Zeng Rongyao , Yang Jinfeng , Xu Zebo , Jin Shaoxiong , Wei Guan 
Published: February 2024

BackgroundA novel risk scoring system, predicated on DNA damage response (DDR), was developed to enhance prognostic predictions and potentially inform the creation of more effective therapeutic protocols for sepsis.MethodsTo thoroughly delineate the expression profiles of DDR markers within the context of sepsis, an analytical approach utilizing single-cell RNA-sequencing (scRNA-seq) was implemented. Our study utilized single-cell analysis techniques alongside weighted gene co-expression network analysis (WGCNA) to pinpoint the genes that exhibit the most substantial associations with DNA damage response (DDR). Through Cox proportional hazards LASSO regression, we distinguished DDR-associated genes and established a risk model, enabling the stratification of patients into high- and low-risk groups. Subsequently, we carried out an analysis to determine our model’s predictive accuracy regarding patient survival. Moreover, we examined the distinct biological characteristics, various signal transduction routes, and immune system responses in sepsis patients, considering different risk categories and outcomes related to survival. Lastly, we conducted experimental validation of the identified genes through in vivo and in vitro assays, employing RT-PCR, ELISA, and flow cytometry.ResultsBoth single-cell RNA sequencing (scRNA-seq) and bulk transcriptomic analyses have demonstrated a strong correlation between DNA damage response (DDR) levels and sepsis prognosis. Specific cell subtypes, including monocytes, megakaryocytes, CD4+ T cells, and neutrophils, have shown elevated DDR activity. Cells with increased DDR scores exhibited more robust and numerous interactions with other cell populations. The weighted gene co-expression network analysis (WGCNA) and single-cell analyses revealed 71 DDR-associated genes. We developed a four-gene risk scoring system using ARL4C, CD247, RPL7, and RPL31, identified through univariate COX, LASSO COX regression, and log-rank (Mantel-Cox) tests. Nomograms, calibration plots, and decision curve analyses (DCA) regarding these specific genes have provided significant clinical benefits for individuals diagnosed with sepsis. The study suggested that individuals categorized as lower-risk demonstrated enhanced infiltration of immune cells, upregulated expression of immune regulators, and a more prolific presence of immune-associated functionalities and pathways. RT-qPCR analyses on a sepsis rat model revealed differential gene expression predominantly in the four targeted genes. Furthermore, ARL4C knockdown in sepsis model in vivo and vitro caused increased inflammatory response and a worse prognosis.ConclusionThe delineated DDR expression landscape offers insights into sepsis pathogenesis, whilst our riskScore model, based on a robust four-gene signature, could underpin personalized sepsis treatment strategies.