bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breast cancer oestrogen independence mediated by BCAR1 or BCAR3 genes is transmitted through mechanisms distinct from the oestrogen receptor signalling pathway or the epidermal growth factor receptor signalling pathway
Authors: Dorssers Lambert CJ , van Agthoven Ton , Brinkman Arend , Veldscholte Jos , Smid Marcel , Dechering Koen J 
Published: November 2004

Abstract
Introduction
Tamoxifen is effective for endocrine treatment of oestrogen receptor-positive breast cancers but ultimately fails due to the development of resistance. A functional screen in human breast cancer cells identified two BCAR genes causing oestrogen-independent proliferation. The BCAR1 and BCAR3 genes both encode components of intracellular signal transduction, but their direct effect on breast cancer cell proliferation is not known. The aim of this study was to investigate the growth control mediated by these BCAR genes by gene expression profiling.

Methods
We have measured the expression changes induced by overexpression of the BCAR1 or BCAR3 gene in ZR-75-1 cells and have made direct comparisons with the expression changes after cell stimulation with oestrogen or epidermal growth factor (EGF). A comparison with published gene expression data of cell models and breast tumours is made.

Results
Relatively few changes in gene expression were detected in the BCAR-transfected cells, in comparison with the extensive and distinct differences in gene expression induced by oestrogen or EGF. Both BCAR1 and BCAR3 regulate discrete sets of genes in these ZR-75-1-derived cells, indicating that the proliferation signalling proceeds along distinct pathways. Oestrogen-regulated genes in our cell model showed general concordance with reported data of cell models and gene expression association with oestrogen receptor status of breast tumours.

Conclusions
The direct comparison of the expression profiles of BCAR transfectants and oestrogen or EGF-stimulated cells strongly suggests that anti-oestrogen-resistant cell proliferation is not caused by alternative activation of the oestrogen receptor or by the epidermal growth factor receptor signalling pathway.
Health professional student attitudes towards people with disability
Authors: Tervo Raymond C , Palmer Glen 
Published: November 2004

Objective: To investigate health professional student attitudes toward people with disability. It was hypothesized that attitude and comfort would differ by discipline. Further, it was hypothesized that factors such as gender and background in disability would influence these attitudes and their ease in dealing with difficult encounters in rehabilitation. Design: A cross-sectional survey design. Setting: University of South Dakota (USD). Participants: Three hundred and thirty-eight students were surveyed. Main outcome measures: (a) Attitude Toward Disabled Persons (ATDP) scale; (b) Scale of Attitudes Toward Disabled Persons (SADP); (c) Rehabilitation Situations Inventory (RSI). Results: There were differences among student groups on the attitude scales and factor scores. All students' attitudes were less positive than SADP norms and nursing students held the least positive opinions. There were no attitudinal differences by gender. Those with a background in disability held more positive attitudes. Years of experience and hours per week employed predicted comfort with challenging rehabilitation situations. Inconsiderate treatment by staff, inappropriate sexual overtures and aggressive behaviour by patients were stated to be most challenging. Conclusion: Health professional students hold less positive attitudes than SADP norms. Nursing undergraduate students were at greater risk of holding negative attitudes. Work experience was important for comfort with challenging rehabilitation situations. Specific educational experiences are needed to promote more positive attitudes.
The effect of cervical traction combined with conventional therapy on grip strength on patients with cervical radiculopathy
Authors: Joghataei Mohammad Taghi , Arab Amir Massoud , Khaksar Hossein 
Published: November 2004

Objective: To determine if combining intermittent cervical traction with conventional physical therapy methods is more effective than using conventional approaches alone in the improvement of the grip strength and treatment of the patients with unilateral cervical 7 (C7) radiculopathy. Design: Randomized controlled trial (RCT). Setting: An outpatient physical therapy clinic, University of Social Welfare and Rehabilitation Science, Iran. Patients: A sample of convenience of 30 patients with unilateral C7 radiculopathy participated in this study. Patients were assigned randomly to a control (N=15, mean age=46.939 ±5.32) and an experimental group (N=15, mean age=47.53 ±5.6). Interventions: Electrotherapyu/exercise treatment for control group and combined cervical traction and electrotherapyu/exercise for experimental group. Ten physical therapy sessions, three times a week for each group. Main outcome measures: Grip strength as an appropriate objective parameter was measured before treatment and after 5 and 10 treatment sessions. Results: Statistical analysis (paired t-test) revealed significant increase in grip strength after 10 treatment sessions in control (p<0.01) and experimental group (p<0.01) compared with pretreatment score. In the ANCOVA, controlling for pretest scores, no significant difference was found between the two groups in the after 10 treatment sessions grip score (p=0.65). However, the change in grip strength after five sessions was significantly greater for the experimental group than for the control group (P=0.04). Conclusions: The application of cervical traction combined with electrotherapy and exercise produced an immediate improvement in the hand grip function in patients with cervical radiculopathy.
Effects of acupuncture and placebo TENS in addition to exercise in treatment of rotator cuff tendinitis
Authors: Razavi Mahnaz , Jansen Gunilla Brodda 
Published: November 2004

Objective: To compare the effect of acupuncture with placebo transcutaneous electrical nerve stimulation (TENS) when added to the exercise treatment of rotator cuff tendinitis with respect to pain, shoulder movements and function. Design: Prospective alternate allocation controlled trial. Setting: Outpatient department. Patients: Thirty-three patients (12 women and 21 men) were included in the study. All had clinically diagnosed rotator cuff tendinitis. Intervention: Both groups underwent a standardized training programme. Each patient received in addition either 10 treatments with acupuncture or placebo TENS, 1-2 times per week. Main outcome measures: The parameters investigated were intensity of pain (measured with visual analogue scale), active, passive as well as functional movements in the shoulder (hand in neck (HIN) and pour out of a pot (POP)). Patients were tested before treatment, after treatment and at a six-month follow-up. Medicine intake, ability to lie on the affected side and sleep disturbances were evaluated. A subjective assessment was made after the treatment and at follow-up. Results: Sixteen patients had acupuncture, 17 placebo TENS. Eight patients endured pain at rest in the placebo TENS group, and 10 in the acupuncture group. After treatment both groups improved, the improvement persisted at the six-month follow-up. Both groups increased range of movement. Except for the functional test HIN in the acupuncture group, there were no differences between the groups regarding other parameters investigated directly after treatment or at six-month follow-up. Conclusion: There is no difference between the effect of additional acupuncture treatment and placebo TENS in the treatment of rotator cuff tendinitis.
Assessment for Chronic Kidney Disease Service in High-Risk Patients at Community Health Clinics
Authors: Patel Harita R , Pruchnicki Maria C , Hall Laura E 
Published: November 2004

BACKGROUND: Chronic kidney disease (CKD) poses significant public health concerns. Early identification and interventions can help prevent or slow progression to end-stage renal disease. OBJECTIVE: To characterize CKD in high-risk indigent patients in a primary care setting and evaluate opportunities for pharmacists to work collaboratively with physicians to improve medication use and CKD patient outcomes. METHODS: Medical records of 200 patients with diabetes mellitus and/or hypertension were reviewed by the clinical pharmacist. Estimated glomerular filtration rate (creatinine clearance [Clcr]) and urinalysis were used to identify and stage CKD according to published guidelines. Glycosylated hemoglobin concentrations and blood pressures were recorded. The pharmacist evaluated medications for possible drug-related problems (DRPs), made therapeutic recommendations, and evaluated the acceptance rate by physicians. RESULTS: One hundred nineteen patients met inclusion criteria, and a total of 68.9% met CKD criteria: stage 1, 16.0%; stage 2, 20.2%; stage 3, 25.2%; stage 4, 1.7%; stage 5, 0.8%; and not stageable, 5.0%. A total of 381 DRPs were identified, averaging 3.2 (1.7) per patient (range 0–11). The number of DRPs correlated with Clcr (r = −0.25; p = 0.007). Therapeutic recommendations included change of drug, dose and/or interval adjustment of the current drug, discontinuation of nonsteroidal antiinflammatory drugs, additional laboratory monitoring, meeting goal blood pressure and glycosylated hemoglobin, adding renoprotective drug and/or low-dose aspirin, and nephrologist referral. Fewer than half (40.9%) of the recommendations were accepted or accepted with modifications, and an approximately equal percentage were not accepted by the physicians. CONCLUSIONS: CKD prevalence was high among the patients evaluated here. New guidelines are available to assist in managing CKD ambulatory patients. Pharmacist collaboration with physicians may optimize CKD screening in high-risk patients and improve medication usage.
A multivariate biomarker-based model predicting population-level responses of Daphnia magna
Authors: De Coen Wim M. , Janssen Colin R. 
Published: November 2004

Abstract
A multivariate model is proposed relating short-term biomarker measurements in Daphnia magna to chronic effects (21-d exposure) occurring at the population level (time to death, mean brood size, mean total young per female, intrinsic rate of natural increase, net reproductive rate, and growth). The results of the short-term exposure (48h-96 h) to eight model toxicants (cadmium, chromium, mercury, tributyl tin, linear alkylsulfonic acid, sodium pentachlorophenolate, lindane, and 2,4-dichloro-phenoxyacetic acid) on the following biomarkers were used for the multivariate model: digestive enzymes (amylase, cellulase, β-galactosidase, trypsin, and esterase), enzymes of the intermediary metabolism (glycogen phosphorylase, glucose-6-phosphate de-hydrogenase, pyruvate kinase, lactate dehydrogenase, and isocitrate dehydrogenase), cellular energy allocation (CEA) (protein, carbohydrate, and lipid content and electron transport activity), and DNA damage and antioxidative stress activity. Using partial least squares to latent structures (PLS), a two-component model was obtained with R2 of 0.68 and a Q2 value of 0.60 based on the combined analysis of a limited number of the 48- and 96-h biomarker responses. For the individual population-level responses, the R2 values varied from 0.66 to 0.77 and the Q2 values from 0.52 to 0.69. Energy-related biomarkers (cellular energy allocation, lipid contents, anaerobic metabolic activity—pyruvate kinase, and lactate dehydrogenase), combined with parameters related to oxidative stress (catalase) and DNA damage measured after 48 and 96 h of exposure, were able to predict long-term effects at higher levels of biological organization.
Toxicity of cadmium, endosulfan, and atrazine in adrenal steroidogenic cells of two amphibian species, Xenopus laevis and Rana catesbeiana
Authors: Goulet Benoǐt N. , Hontela Alice 
Published: November 2004

Abstract
The effects of cadmium, endosulfan, and atrazine on corticosterone secretion and viability of adrenal cells of African clawed frog (Xenopus laevis) and bullfrog (Rana catesbeiana) were assessed in vitro using a new bioassay. The bioassay relies on stimulation with adrenocorticotropic hormone (ACTH), the endogenous secretagogue for corticosterone secretion, and with dibutyryl cyclic adenosine monophosphate (dbcAMP), an analogue of cAMP, to pinpoint the site of action of the xenobiotics within the steroidogenic cell. To compare the test toxicants according to their endocrine-disrupting potential, the lethal concentration needed to kill 50% of the cells:effective concentration of 50% (LC50:EC50) ratio was calculated, with LC50 as the concentration that kills 50% of the steroidogenic cells and the EC50 as the concentration that impairs corticosterone secretion by 50%. The higher the ratio, the higher the potential for endocrine disruption. Atrazine had no affect on cell viability and on corticosterone secretion in X. laevis, but its endocrine-disrupting potential was high in R. catesbeiana. The LC50:EC50 ratio for cadmium and endosulfan in X. laevis was 26.07 and 1.23, respectively, and for atrazine, cadmium, and endosulfan in R. catesbeiana it was 909, 41, and 3, respectively. The dbcAMP did not restore corticosterone secretion in the cells exposed to the test toxicants in both species. Our study suggests that the secretory capacity of adrenal cells of amphibians can be impaired by environmental chemicals, especially atrazine in the bullfrog, and that these adrenotoxicants disrupt the enzymatic pathways leading to corticosterone secretion downstream from the step-generating cAMP.
Assessment of the freshwater annual fish Cynopoecilus melanotaenia as a toxicity test organism using three reference substances
Authors: Arenzon Alexandre , Pinto Régis Fontana , Colombo Patrick , Raya-Rodriguez Maria Teresa 
Published: November 2004

Abstract
This study presents a preliminary evaluation of the use of the Brazilian fish Cynopoecilus melanotaenia as a test organism in toxicity tests. The cryptobiotic stage presented by the eggs of fish C. melanotaenia can overcome the difficulty of continuously keeping cultures and recruiting healthy animals in sufficient numbers to be used in toxicity tests. In order to determine the applicability of this species as a test organism, three different reference substances were evaluated in 96-h acute toxicity tests: Copper sulfate (CuSO4·5H2O), sodium dodecil sulfate (C12H25NaO4S), and sodium chloride (NaCl). Sensitivity ranged as follows: copper sulfate (0.05-0.13 mg/L), sodium dodecil sulfate (10.7-19.0 mg/L), and sodium chloride (1.44-1.96 g/L). We conclude that C. melanotaenia shows potential as a test organism in toxicity tests; however, further research should be conducted with other substances and should be compared with the research on other species before we can reach more conclusive results.
Effects of pore-water ammonia on in situ survival and growth of juvenile mussels (;Lampsilis cardium); in the St. Croix Riverway, Wisconsin, USA
Authors: Bartsch Michelle R. , Newton Teresa J. , Allran John W. , O'Donnell Jonathan A. , Richardson William B. 
Published: November 2004

Abstract
We conducted a series of in situ tests to evaluate the effects of pore-water ammonia on juvenile Lampsilis cardium in the St. Croix River (WI, USA). Threats to this river and its associated unionid fauna have accelerated in recent years because of its proximity to Minneapolis–St. Paul, Minnesota, USA. In 2000, caged juveniles were exposed to sediments and overlying water at 12 sites for 10 d. Survival and growth of juveniles was significantly different between sediment (mean, 47%) and water column (mean, 86%) exposures; however, these effects were unrelated to pore-water ammonia. During 2001, juveniles were exposed to sediments for 4, 10, and 28 d. Pore-water ammonia concentrations ranged from 0.3 to 62.0 μg NH3-N/L in sediments and from 0.5 to 140.8 μg NH3-N/L within exposure chambers. Survival (mean, 45, 28, and 41% at 4, 10, and 28 d, respectively) and growth (range, 3–45 μm/d) of juveniles were highly variable and generally unrelated to ammonia concentrations. Although laboratory studies have shown unionids to be quite sensitive to ammonia, further research is needed to identify the route(s) of ammonia exposure in unionids and to understand the factors that contribute to the spatial variability of ammonia in rivers.
Growth and nitrite and nitrous oxide accumulation of Paracoccus denitrificans ATCC 19367 in the presence of selected pesticides
Authors: Sáez Florentina , Pozo Clementina , Gómez Miguel Angel , Rodelas Belén , Gónzalez-López Jesús 
Published: November 2004

Abstract
The effects of the application of eight pesticides (aldrin, lndane, dimetoate, methylparathion, methidation, atrazine, simazine, and captan) on growth, respiratory activity (as CO2 production), denitrifying activity (as N2O released), and nitrite accumulation in the culture medium by Paracoccus denitrificans strain ATCC 19367 were studied. The fungicide captan totally inhibited growth and biological activity of P. denitrificans, while the rest of the tested pesticides delayed the growth and CO2 release of P. denitrificans but did not drastically affect the bacterial growth or respiratory capacity after 96 h of culture. The denitrifying activity of P. denitrificans ATCC 19367 (as N2O released) was negatively affected by all tested pesticides. The release of N2O was strongly inhibited by several organochlorinated and organophosphorated insecticides (aldrin, lindane, dimetoate, and methidation), which led to high accumulation of nitrite in the surrounding medium. Atrazine decreased N2O release after 48 h of culture because of negative effects on growth, and methylparathion and simazine delayed the onset of N2O release by P. denitrificans. These three pesticides reduced the accumulation of NO2 compared to unamended control cultures.
Reduced nicotinamide adenine dinucleotide phosphate and the higher incidence of pollution-induced liver cancer in female flounder
Authors: Koehler Angela , Van Noorden Cornelis J. F. 
Published: November 2004

Abstract
In biological effect monitoring programs, induction of biotransformation and detoxification enzymes is used as a biomarker for pollution. Yet sex differences are usually neglected in the availability of reduction equivalents needed in these metabolic pathways and may affect biomarker responses. For example, female flounder have a threefold higher incidence of macroscopic liver nodules than males of the same age class in polluted environments of the North Sea that progress toward carcinomas, whereas tumors in males virtually never develop into cancer. Evidence is presented in this review that NADPH plays a significant role in this sex-related response to xenobiotics in liver of flounder. The NADPH is needed for biosynthesis, particularly of lipids and lipoproteins, and detoxification processes such as one-electron and two-electron biotransformation and conjugation and, therefore, its availability as substrate determines biomarker responses. Biotransformation of xenobiotics is more strongly induced and conjugation processes are less affected in male flounder liver during exposure. In female liver, NADPH is required for production of the yolk precursor protein vitellogenin for oocyte production. The latter process has a higher priority than the NADPH-requiring detoxification processes because reproductive success is more relevant in evolutionary perspectives than the survival of the individual female. The data reviewed here suggest that these sex-related differences in NADPH metabolism are a major cause of the higher incidence of liver cancer in female flounder in polluted environments.
A microscaled mercury saturation assay for metallothionein in fish
Authors: Shaw-Allen Patricia , Elliott Muriel , Jagoe Charles H. 
Published: November 2004

Abstract
A mercury (Hg) saturation assay for measuring metallothionein (MT) in fish liver was modified by optimizing binding conditions to minimize the mercury and tissue consumed. The revised method uses stable Hg at low concentrations instead of 203Hg. At the reduced Hg concentrations used, MT concentrations in livers homogenized in saline appeared to increase systematically with dilution in both bluegill sunfish (Lepomis macrochirus) and largemouth bass (Micropterus salmoides). This error suggested a binding limitation due to sulfhydryl oxidation or competition for and removal of mercury by non-MT proteins. Homogenizing tissues in trichloroacetic acid (TCA) eliminated the interference. To further evaluate the method, the protocol was tested in the laboratory and field. Metallothionein in bluegill injected with 0.6 mg/kg zinc chloride increased at a rate of 0.03 nmole MT/g liver/ h (r2 = 0.53, p = 0.001). Linearity improved when data were corrected for protein content (r2 = 0.74, p &lt; 0.0001). Metallothionein levels in bluegill from a coal ash-contaminated environment were significantly increased over that of hatchery-reared sunfish (F = 20.17, p = 0.0003). The microscaled procedure minimizes concerns related to radioisotope use and waste generation while retaining the high sensitivity of the 203Hg assay.
Effects of carbaryl on green frog (Rana clamitans) tadpoles: Timing of exposure versus multiple exposures
Authors: Boone Michelle D. , Bridges Christine M. 
Published: November 2004

Abstract
The majority of studies on pesticide impacts have evaluated the effects of single exposures. However, multiple exposures to a pesticide may be more prevalent. The objective of our study was to determine how multiple exposures versus single exposure at different times during development affected survival to metamorphosis, tadpole survival, tadpole mass, and tadpole developmental stage of green frog (Rana clamitans) tadpoles reared at low and high density in outdoor cattle tank ponds. Tadpoles were exposed to carbaryl zero, one, two, or three times at 14-d intervals. We applied single doses of carbaryl at one of three times, specifically during early, mid, or late development. Overall, we found that multiple exposures had a greater impact than single exposures during development. More individuals reached metamorphosis in ponds exposed to multiple doses of carbaryl compared with controls, indicating that the presence of carbaryl stimulated metamorphosis. The presence of carbaryl in the aquatic environment also resulted in more developed tadpoles compared with controls. Tadpoles in control ponds did not reach metamorphosis and were less developed than individuals exposed to carbaryl; this effect indicates that, under ideal conditions, green frogs could overwinter in ponds so that greater size could be attained before metamorphosis in the following spring or summer. Our study demonstrated the importance of including realistic application procedures when evaluating the effects of a pesticide and that multiple exposures to a short-lived pesticide are more likely to affect an amphibian population.