bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emerging variants develop total escape from potent monoclonal antibodies induced by BA.4/5 infection
Authors: Stuart David , Liu Chang , Das Raksha , Dijokaite-Guraliuc Aiste , Zhou Daming , Mentzer Alexander , Supasa Piyada , Selvaraj Muneeswaran , Duyvesteyn Helen , Ritter Thoms , Temperton Nigel , Klenerman Paul , Dunachie Susanna , Paterson Neil , Williams Mark , Hall Dave , Fry Elizabeth , Mongkolsapaya Juthathip , Ren Jingshan , Screaton Gavin 
Published: December 2023

Abstract
The rapid evolution of SARS-CoV-2 is driven in part by a need to evade the antibody response in the face of herd immunity. Here, we isolate spike binding monoclonal antibodies (mAbs) from vaccinees who suffered vaccine break-through infections with Omicron sub lineages BA.4 or BA.5. 28 potent antibodies were isolated and characterised functionally, and in some cases structurally. Since the emergence of BA.4/5 SARS-CoV-2 has continued to accrue mutations in the S protein, to understand this we characterize neutralization of a large panel of variants and demonstrate a steady attrition of neutralization by the panel of BA.4/5 mAbs culminating in total loss of function with recent XBB.1.5.70 variants containing the so-called ‘FLip’mutations at positions 455 and 456. Interestingly, activity of some mAbs is regained on the recently reported variant BA.2.86.
Co-expression network analysis and identification of core genes in the interaction between wheat and Puccinia Striiformis f. sp. tritici
Authors: Wang Yibo , Zhang Ke , Chen Dan , Liu Kai , Chen Wei , He Fei , Tong Zhijun , Luo Qiaoling 
Published: December 2023

Abstract

The epidemic of stripe rust, caused by the pathogen Puccinia Striiformis f. sp. tritici (Pst), would reduce wheat (Triticum aestivum) yields seriously. Traditional experimental methods are difficult to discover the interaction between wheat and Pst. Multi-omics data analysis provides a new idea for efficiently mining the interactions between host and pathogen. We used 140 wheat-Pst RNA-Seq data to screen for differentially expressed genes (DEGs) between disease-resistant and disease-susceptible samples, and carried out Gene Ontology (GO) enrichment analysis. Based on this, we constructed a gene co-expression network, identified the core genes and interacted gene pairs from the conservative modules. Finally, we checked the distribution of Nucleotide-binding and leucine-rich repeat (NLR) genes in the co-expression network and drew the wheat NLR gene co-expression network. In order to provide accessible information for related researchers, we built a web-based visualization platform to display the data. Based on the analysis, we found that various heat shock proteins (HSPs), protein kinases, and glycosidases frequently appeared in the network. They were likely to be involved in the biological processes of Pst infecting wheat. We also found that HSPs was significantly co-expressed in wheat and Pst, suggesting that there might be direct or indirect interactions between them. This study can assist scholars in conducting studies on the pathogenesis and help to advance the investigation of wheat-Pst interaction patterns.
Development and validation of a new model for predicting the recurrence of hepatocellular carcinoma after liver transplantation：a single center experience with 325 cases
Authors: Duan Shaoxian , Hou Dingcong , Xie Yan , Jiang Wen-tao 
Published: December 2023

Abstract
Background
Hepatocellular carcinoma recurrence after liver transplantation has a dismal prognosis. We aimed to develop a new model for effectively predicting the recurrence of hepatocellular carcinoma after liver transplantation.
Methods
We retrospectively analyzed the clinical data of 325 patients with hepatocellular carcinoma who underwent a first orthotopic liver transplantation at Tianjin First Central Hospital.Univariate and multivariate analyses were used to identify potential predictors. A new scoring model based on the identified predictors was developed and validated.
Results
Cox multivariate analysis revealed tumor number ≥ 2, ki67 labeling index > 0.23, preoperative neutrophil-lymphocyte ratio > 4, maximum tumor diameter > 5.25 cm, and preoperative serum alpha-fetoprotein > 88.33 ng/ml as independent risk factors for postoperative recurrence in the development cohort. We developed a new scoring model. The patients were divided into high-risk and low-risk groups according to the new scoring model.The 3-year recurrence-free survival rates after liver transplantation in the high-risk group were significantly lower than those in the low-risk group (P < 0.001) and there was a significant difference in the cumulative incidence of recurrence between the two risk groups on competing-risk regression in the two cohorts. Receiver operating characteristic curve analysis showed that the area under the curve was 0.870 in the development cohort and 0.772 in the validation cohort. Hanley–McNeil analysis demonstrated that the new model was superior to the Milan criteria (P = 0.040) for predicting tumor recurrence after liver transplantation.
Conclusion
The model effectively predicted hepatocellular carcinoma recurrence after liver transplantation, and it is promising as a reference for clinical decision-making.
Evaluation of picture change effects on fractional occupation number states in noble gas atoms
Authors: Takashima Chinami , Nakai Hiromi 
Published: December 2023

Abstract
In the two-component relativistic density functional theory, the picture change error (PCE), which originates from insufficient transformation of operators, should be corrected. In this study, we examine the PCE in the fractional occupation number (FON) state based on the spin-free infinite-order two-component Hamiltonian. The PCE for the total and orbital energy changes is estimated with respect to the FON electrons of the highest occupied molecular orbital and 1s core orbital in noble gas atoms. PCE is significant in core orbitals in heavy elements but relatively small in light elements and valence orbitals. The delocalization error, which can be represented by the total energy deviation from the behavior of the exact energy, is overestimated by the lack of picture change correction of the two-electron operator and underestimated by that of the density operator. Corresponding to these results, the PCE influences the value of orbital energies and slope of orbital energy change to FON.
A Comparative Cross-Sectional Study on Process Model Comprehension driven by Eye Tracking and Electrodermal Activity
Authors: Winter Michael , Bredemeyer Cynthia , Reichert Manfred , Neumann Heiko , Pryss Rüdiger 
Published: December 2023

Abstract

The continual digital transformation in various domains yielded promising opportunities, especially in the healthcare industry. Thereby, a seamless integration of healthcare expertise with information technology (IT) is essential to benefit further from the potentials of digitization (e.g., generate new communication and collaboration synergies). A lingua franca (i.e., a common language that enables understandable information interchange between healthcare practitioners and IT) is vital to this endeavor. The Business Process Model and Notation (BPMN) 2.0 is a promising option for such a lingua franca, according to earlier studies. In order to assess BPMN 2.0's potential as a lingua franca, a cross-sectional follow-up study with N = 31 participants was carried out on this foundation. Two process models for dental appointment and vaccination were explained to the participants. Their cognitive load, skin conductivity (i.e., electrodermal activity), and gaze movements (i.e., eye tracking) were all monitored simultaneously. The examined performance indicators reinforce previous conclusions, showing that it is feasible to adequately understand process model semantics, which confirms the effectiveness of BPMN 2.0 as a universal language. However, the significant focus should be on minimizing the inherent complexity of process model syntactics and pragmatics. The outcomes additionally demonstrated the potential for assessing eye tracking and electrodermal activity in this context.
Endogenous organization form and trade costs
Authors: Choi Kangsik 
Published: December 2023

Abstract
We ananlyze the endogenous firms' choice of organization form in the presence of endogenous freight rates, port fees with the public (or asymmetric) port ownership. We found that regardless of port ownership, one firm provides corporate incentives (i.e., U-form), and other firm provides divisional incentives (i.e., M-form). Thus, choosing U-form (resp. M-form) in the asymmetric organization for firm and producer surplus is profitable (resp. unprofitable), but for the government and consumer surplus is socially undesirable (resp. desirable). Furthermore, we present some extensions to check the robustness of the obtained results.
JEL Classi cation: D43, F12, F13, L13, M16.
BENet: Boundary-Enhanced Network for Real-time Semantic Segmentation
Authors: Lei Xiaochun , Chen Zeyu , Yu Zhaoxin , Jiang Zetao 
Published: December 2023

Abstract

In the realm of real-time semantic segmentation, deep neural networks have demonstrated promising potential. However, current methods face challenges when it comes to accurately segmenting object boundaries and small objects. This limitation is partly attributed to the prevalence of convolutional neural networks, which often involve multiple sequential down-sampling operations, resulting in the loss of fine-grained details. To overcome this drawback, we introduce BENet, a real-time semantic segmentation network with a focus on enhancing object boundaries. The proposed BENet integrates two key components: the Boundary Extraction Module (BEM) and the Boundary Adaption Layer (BAL). The proposed BEM efficiently extracts boundary information, while the BAL guides the network using this information to preserve intricate details during the feature extraction process. Furthermore, to address the challenges associated with poor segmentation of elongated objects, we introduce the Strip Mixed Aggregation Pyramid Pooling Module (SMAPPM). This module employs strip pooling kernels to effectively expand the contextual representation and receptive field of the network, thereby enhancing overall segmentation performance. Our experiments conducted on a single RTX 3090 GPU show that our method achieves an mIoU of 79.4% at a speed of 45.5 FPS on the Cityscapes test set without ImageNet pre-training.
Sonographic temporary nephromegaly in children during the initial febrile urinary tract infection is a significant prognostic factor for recurrent infection
Authors: Ishimori Shingo , Fujimura Junya , Nakanishi Keita , Hattori Kengo , Hirase Satoshi , Matsunoshita Natsuki , Kamiyoshi Naohiro , Okizuka Yo 
Published: December 2023

Abstract

There is no available data on the relationship between sonographic temporary nephromegaly in children with the initial episode of febrile urinary tract infection (fUTI) and recurrent fUTI, with a focus on whether nephromegaly is temporary or not. A multicenter retrospective cohort study on children who underwent renal ultrasound during initial fUTI was conducted between 2013 and 2020. Sonographic temporary nephromegaly is defined as increased renal length during initial fUTI following normal length of a kidney after completion of antibiotic treatment. This study included 343 children. In comparison to children without sonographic temporary nephromegaly (n = 307), duration of fever and intravenous antibiotics were significantly longer, and the level of serum CRP, creatinine and the proportion of children had recurrent fUTI were significantly higher in those with sonographic temporary nephromegaly (n = 36). In an additional analysis of 100 patients receiving voiding cystourethrography, Logistic regression model confirmed that vesicoureteral reflux (VUR) was significantly correlated with temporary nephromegaly and recurrence. The indication receiving cystourethrography in 9 of 16 with VUR who had temporary nephromegaly was recurrent fUTI. Our results suggest that sonographic temporary nephromegaly on initial infection could be a predictive factor for recurrence and VUR, and children with this factor could detect VUR before recurrence.
Latent profile analysis of benefit finding among among family caregivers of Chinese older adults with disability and its influencing factors
Authors: Liu Zijing , Sun Longfeng 
Published: December 2023

Abstract
Objective To explore the characteristics of potential categories of benefit finding among family caregivers of Chinese older adults with disability, and to analyze the differences in the characteristics of different categories of caregivers and related influencing factors.
Methods Convenience sampling was used to consecutively select family caregivers of disabled older adults in Zhongshan Community, Bayi Community, Beishi Community Health Service Center, and Neurology, Rehabilitation, and Geriatrics Departments of the First Affiliated Hospital of China Medical University in Shenyang City, Liaoning Province, China, from October 2022-June 2023 as the survey respondents. General Information Questionnaire, Sense of Coherence-13(SOC-13),Simplified Copying Style Questionnaire(SCSQ) and Benefit Finding Sacle(BFS)were used to conduct the survey. Mplus 8.3 and SPSS25.0 software were applied for Latent profile analysis and statistical analysis.
Results A total of 218 family caregivers of older adults with disability were surveyed, and the caregiver benefit score was 57.00 (47.00, 67.25); the caregiver benefit was expressed in three different potential categories, named "low-level benefit finding group" (43.58%), "medium-level benefit finding group" (43.58%), and "high-level benefit finding group" (12.84%). Ordered logistic regression analyses showed that years of caregiving experience of family caregivers of the disabled elderly, work status, family functioning, and coping styles were the factors influencing the potential profile categories of benefit finding (P < 0.05).
Conclusion The benefit finding of family caregivers of older adults with disability are at a low level and are significantly heterogeneous. It is recommended that clinicians and community workers focus on family caregivers of disabled older adults with short caregiving years and those who are working, and guide family caregivers of disabled older adults to improve the family support system, adopt positive coping styles, promote the perception of benefits, and improve the life satisfaction of disabled older adults.
Modeling of a hydrogen storage tank charging process
Authors: Milewski Jaroslaw , Szczesniak Arkadiusz , Dybiński Olaf , Szałek Andrzej 
Published: December 2023

Abstract

The paper presents a 0-D model of a hydrogen storage tank which is calibrated based on available experimental data. The results obtained are used for operation estimates of higher volume tanks. Increased volume should make hydrogen storage easier, and additional improvements can be made by appropriate selection of the inlet valve and thicker tank walls. It seems justifiable to increase both operating and filling pressures, due to the relatively small energy profits and much more complex charging process. Based on the energy balance, the charging process is endothermic. Neither Joule-Thomson heat nor kinetic energy of the hydrogen discharged allow one to obtain the high temperature reported. There is no experimental data with adequate quality to validate a high-volume storage hydrogen tank. Since the available data correspond to small size tanks and frequently contain contradictions, further experimental investigations are needed in the field.
The protective effect of fluoxetine on hippocampal oligodendrocytes in AD mice: a potential mechanism involving S100B
Authors: Zhang Yi , Jiang Lin , Tang Yong , Chen Weixian , Chao Feng-lei 
Published: December 2023

Abstract
Fluoxetine (FLX) plays a crucial role in delaying the progression of Alzheimer's disease (AD) and opens up new avenues for investigating the mechanisms of AD. Previous research has shown that FLX has a protective effect on oligodendrocytes in the hippocampus of transgenic AD mice. However,the mechanism underlying this effect remains unclear. In this study, we treated 8-month-old APP/PS1 transgenic mice with FLX for 10 weeks and examined the behavioral changes, the levels of S100B protein, the numbers of hippocampus oligodendrocytes and the co-localization of S100B in oligodendrocytes and astrocytes in the hippocampus. Furthermore, we analyzed the damaging effects of exogenous S100B on an oligodendrocyte cell line in vitro and investigated whether fluoxetine could reverse these damages. Our findings demonstrate that FLX delays the decline of hippocampus-dependent spacial learning and memory abilities and the loss of hippocampal oligodendrocytes in AD mice. We also observed a positive correlation between the total number of hippocampal oligodendrocytes and the hippocampal volume. Additionally, FLX reduces hippocampal S100B levels and the expression of S100B in both astrocytes and oligodendrocyte progenitor cells in AD mice. Our cell-based experiments indicate that FLX prevents S100B-induced impairments in oligodendrocyte differentiation and maturation. Moreover, the protective effect of FLX may be associated with the preservation of mitochondrial autophagy and the upregulation of transcription and differentiation factors. Overall, our results highlight the potential mechanisms underlying the protective effect of fluoxetine on S100B-induced impairment of oligodendrocyte differentiation and maturation in AD, and propose a new target for the prevention and treatment of AD.
PREVALENCE OF BURNOUT AMONG HEALTHCARE PROFESSIONALS: A SURVEY AT FORTPORTAL REGIONAL REFERRAL HOSPITAL
Authors: Batanda Ian 
Published: December 2023

Abstract
The work environment in most hospitals is characterized by activities that are strenuous both physically and mentally. These can result in physical and mental exhaustion, which can lead to burnout if not adequately addressed. Burnout among healthcare professionals can negatively affect their clinical decision-making, quality of communication with patients and colleagues as well as their ability to cope with work-related pressure, and ultimately affect the quality of care and patient outcomes. The inclusion of burnout in the 11th revision of the International Classification of Diseases (ICD-11) as an occupational phenomenon indicates that it is an issue of concern in the workplace for which people may need professional attention.
Aim of the study
The aim was to estimate the prevalence of burnout among healthcare professionals at Fort Portal Regional Referral Hospital and to evaluate the linear relationship between the age of workers, their work duration at the hospital, and their burnout score. The possible impact on patient care and the factors contributing to burnout were also evaluated.
Methods:
The study was a descriptive cross-sectional online survey. Participants were selected from the hospital WhatsApp group and invitations to participate were sent to their individual in boxes. Burnout was assessed using the Copenhagen Burnout Inventory.
Results:
Generally, burnout scores ranged from 16% to 86%, with an overall mean burnout score of 57.4%. The notable factors contributing to burnout included imbalances in duty allocation, physically strenuous work, and resource constraints.
Conclusion:
Burnout of varying levels is prevalent across all carders in the hospital, although most healthcare professionals experience moderate burnout. Most of the factors contributing to burnout are within the scope of hospital leadership to address. The possible impact on staff performance and patients’ clinical outcomes is speculative, and additional studies are required.
Roles of Lysine-63-linked ubiquitination on Hif-1α in cell fate decisions between cell proliferation and apoptosis under cadmium effects in epithelial cells
Authors: Chargui Abderrahmen , Belaid Amine , Hashem Abeer , Al-Hazzani Amal A. , Abd_Allah Elsayed Fathi , Hammami Imen , Nahdi Afef , Marzougui Salem , Hofman Paul , Elmay Michèle , Mograbi Baharia 
Published: December 2023

Abstract
Lysine-63-linked ubiquitination -, i.e., The conjugation of a chain of ubiquitins linked through lysine63 has emerged as a key mechanism regulating signal transduction pathways. Although critical, very little information is currently available about how subversion of lysine 63 ubiquitination might contribute to cancers and inflammatory diseases. The present study provides evidence that cadmium, a carcinogenic and toxic substance released into the environment, is a powerful activator of lysine-63 ubiquitination. Indeed, cadmium induces the accumulation of lysine-63-polyubiquitination proteins. Importantly, cadmium-induced ubiquitination does not stem oxidative damage or proteasome impairment. Rather, we demonstrate that cadmium not only activates Lysine 63 ubiquitination but also amplifies its accumulation by overloading the capacity of the autophagy pathway, thus promoting endoplasmic reticulum stress and cell death. At the molecular level, cadmium-induced ubiquitination is correlated with the stabilization of hypoxia inducible factor-1-alpha, a transcription factor. Strikingly, prolonged cell exposure to high cadmium concentrations induces exaggerated lysine-63 ubiquitination that fosters aggresome formation, thus precluding this protein from interacting with its downstream nuclear targets. We therefore propose that the aberrant activation of lysine-63 ubiquitination by the carcinogen cadmium could promote cell proliferation and inflammation at low levels, while high levels lead to cell death.
Investigating the Potential of dietary additives, Spirulina platensis and Curcumin Nanoparticles in Nile Tilapia Fingerlings: A Comprehensive Analysis of Growth, Health, and Immune Function
Authors: Eissa El-Sayed Hemdan , Khattab Marwa S. , Elbahnaswy Samia , Elshopakey Gehad E. , Alamoudi Muna O. , Aljarari Rabab M. , Munir Mohammad Bodrul , Kari Zulhisyam Abdul , Naiel Mohammed A.E. 
Published: December 2023

Abstract

A total of 180 Nile tilapia fingerlings (10 ± 0.5 g) were separated into three groups (n = 60), including a control group and two experimental groups supplemented withSpirulina platensis(SP; 5 g.kg-1) and curcumin nanoparticles (Cu-NPs; 30 mg.kg-1), respectively. After a 56-day feeding study, an evaluation of growth performance, hematological factors, antioxidant activity, biochemical and immunological characteristics, and histological modifications were conducted. The results revealed that the SP- and Cu-NPs supplements significantly improved growth indices, carcass features and biochemical features in both experimental groups compared to the control. Furthermore, the activities of digestive enzymes, comprising amylase, lipase, and protease, were significantly increased in the SP and Cu-NPs groups, unlike the control group. Additionally, supplementation with Cu-NPs and SP significantly enhanced the antioxidant state, as exhibited by increased glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity and decreased activity of malondialdehyde (MDA). The immune response was also significantly improved, with higher serum IgM and lysozyme activity in the SP and Cu-NPs treatments. Therefore, this study provides evidence that dietary supplementation with Cu-NPs and SP efficiently enhances the activity of digestive enzymes, antioxidant capacity, growth and performance, hepatoprotective capabilities, histopathology, and immune responses of Nile tilapia fingerlings.