bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fatigue risk assessment of a Helicopter Emergency Medical Service crew working a 24/7 shift pattern: results of a prospective service evaluation
Authors: Rose C. , ter Avest E. , Lyon R. M. 
Published: November 2023

Abstract
Background
The work of Helicopter Emergency Medical Services (HEMS) teams crosses the boundaries of several high-risk occupations including medicine, aviation, and transport. Working conditions can be challenging and operational demands requires a 24-h rota, resulting in disruption of the normal circadian rhythm. HEMS crews are therefore prone to both mental and physical fatigue. As fatigue in medical providers is linked to poor cognitive performance, degradation of psychomotor skills and error, this study aimed to explore the existence of predictable patterns of crew-fatigue in a HEMS service.

Methods
HEMS medical crew members working a 3-on 3-off forward rotating rota with a 5-week shift cycle were asked to do psychomotor vigilance tests (PVT) as an objective measure of fatigue. PVT testing was undertaken at the start, mid- and at the end of every shift during a full 5-week shift cycle. In addition, they were asked to score subjective tiredness with the Samn-Perelli Fatigue Scale (SPFS), and to keep a Transport Fatigue Assessment shift log, wherein they noted shift characteristics potentially related to fatigue. Primary outcome of interest was defined as the change in PVT and SPFS scores over time.

Results
Mean baseline resting PVT in milliseconds at the start of the study period was 427 [390–464]. There was an overall trend towards higher PVT-scores with shift progression mean [95% CI] PVT at the start of shifts 447 [433–460]; halfway through the shift 452 [440–463]; end of the shift 459 [444–475], p = 0.10), whereas SPFS scores remained constant. Within a 5 week forward-rotating cycle, an overall trend towards a gradual increase in both average PVT (from 436 [238–454] to 460 [371–527, p = 0.68] ms;) and SPFS (from 2.9 [2.6–3.2] to 3.6 [3.1–4.0], p = 0.38) was observed, although significant interindividual variation was present. Reported SPFS scores ≥ 4 (moderate fatigue) were mainly related to workload (number of jobs) and transport mode (car-based shifts).

Conclusion
An overall trend towards a decline in psychomotor vigilance and an increase in self-reported tiredness was found for HEMS crew over a 5-week shift cycle. Using a bespoke predictive fatigue tool on a day-to-day basis could increase fatigue awareness and provide a framework to which relevant mitigating options can be applied.
An accident waiting to happen? Exposing the potential of urogenital schistosomiasis transmission in the Lake Albert region, Uganda
Authors: Tumusiime Julius , Kagoro-Rugunda Grace , Tolo Casim Umba , Namirembe Daisy , Schols Ruben , Hammoud Cyril , Albrecht Christian , Huyse Tine 
Published: November 2023

Abstract
Background
Urogenital schistosomiasis caused by the parasitic blood fluke Schistosoma haematobium is the most common form of that constitutes a majority of over 240 million schistosomiasis cases. The enigmatic absence of urogenital schistosomiasis in Uganda has, until now, been attributed to the absence of substantial populations of suitable snail intermediate hosts.

Methods
Malacological surveys were carried out in 73 sites southeast of Lake Albert, Uganda in October and November 2020. Collected snails were transported to the laboratory for identification. The snails were identified using partial mitochondrial cytochrome c oxidase subunit one and nuclear internal transcribed spacer barcoding. Schistosome infections in snails were also assessed using cercarial shedding and rapid diagnostic PCR techniques.

Results
We found Bulinus globosus and Bulinus nasutus productus, the main intermediate species in the transmission of S. haematobium in mainland East Africa. In this survey, B. globosus was more common than B. nasutus productus, with the former reported at four sites (total count = 188) and the latter reported at one site (total count = 79). Molecular testing revealed a high prevalence of Schistosoma bovis in B. nasutus productus (16%), but no S. haematobium infections were found.

Conclusions
Given the abundance of snail hosts and the risky human water contact behaviours observed, we highlight the potential for urogenital schistosomiasis transmission in the region.

Graphical Abstract
Annotation of cell types (ACT): a convenient web server for cell type annotation
Authors: Quan Fei , Liang Xin , Cheng Mingjiang , Yang Huan , Liu Kun , He Shengyuan , Sun Shangqin , Deng Menglan , He Yanzhen , Liu Wei , Wang Shuai , Zhao Shuxiang , Deng Lantian , Hou Xiaobo , Zhang Xinxin , Xiao Yun 
Published: November 2023

AbstractBackgroundThe advancement of single-cell sequencing has progressed our ability to solve biological questions. Cell type annotation is of vital importance to this process, allowing for the analysis and interpretation of enormous single-cell datasets. At present, however, manual cell annotation which is the predominant approach remains limited by both speed and the requirement of expert knowledge.MethodsTo address these challenges, we constructed a hierarchically organized marker map through manually curating over 26,000 cell marker entries from about 7000 publications. We then developed WISE, a weighted and integrated gene set enrichment method, to integrate the prevalence of canonical markers and ordered differentially expressed genes of specific cell types in the marker map. Benchmarking analysis suggested that our method outperformed state-of-the-art methods.ResultsBy integrating the marker map and WISE, we developed a user-friendly and convenient web server, ACT (http://xteam.xbio.top/ACT/orhttp://biocc.hrbmu.edu.cn/ACT/), which only takes a simple list of upregulated genes as input and provides interactive hierarchy maps, together with well-designed charts and statistical information, to accelerate the assignment of cell identities and made the results comparable to expert manual annotation. Besides, a pan-tissue marker map was constructed to assist in cell assignments in less-studied tissues. Applying ACT to three case studies showed that all cell clusters were quickly and accurately annotated, and multi-level and more refined cell types were identified.ConclusionsWe developed a knowledge-based resource and a corresponding method, together with an intuitive graphical web interface, for cell type annotation. We believe that ACT, emerging as a powerful tool for cell type annotation, would be widely used in single-cell research and considerably accelerate the process of cell type identification.
Impact of increasing morphological information by micro-CT scanning on the phylogenetic placement of Darwin wasps (Hymenoptera, Ichneumonidae) in amber
Authors: Viertler Alexandra , Urfer Karin , Schulz Georg , Klopfstein Seraina , Spasojevic Tamara 
Published: November 2023

AbstractThe correct interpretation of fossils and their reliable taxonomic placements are fundamental for understanding the evolutionary history of biodiversity. Amber inclusions often preserve more morphological information than compression fossils, but are often partially hidden or distorted, which can impede taxonomic identification. Here, we studied four new fossil species of Darwin wasps from Baltic and Dominican amber, using micro computed tomography (micro-CT) scans and 3D reconstructions to accurately interpret and increase the availability of morphological information. We then infer their taxonomic placement in a Bayesian phylogenetic analysis by combining morphological and molecular data of extant and fossil Darwin wasps and evaluate the impact and usefulness of the additional information from micro-CT scanning. The results show that although we gained significant morphological information from micro-CT scanning, especially concerning measurements and hidden dorsal and ventral structures, this did not impact subfamily-level placement for any of the four fossils. However, micro-CT scanning improved the precision of fossil placements at the genus level, which might be key in future dating and diversification analyses. Finally, we describe the four new fossil species as Rhyssa gulliveri sp. nov. in Rhyssinae, Triclistus levii sp. nov. in Metopiinae, Firkantus freddykruegeri gen. et. sp. nov. in Pimplinae and Magnocula sarcophaga gen. et sp. nov. in Phygadeuontinae. The first two species are the first known representatives of the subfamilies Rhyssinae and Metopiinae in amber.
Conservative Fourier spectral method for a class of modified Zakharov system with high-order space fractional quantum correction
Authors: Guo Tao , Xiao Aiguo , Wang Junjie , Li Xueyang 
Published: November 2023

AbstractIn this paper, we consider the Fourier spectral method and numerical investigation for a class of modified Zakharov system with high-order space fractional quantum correction. First, the numerical scheme of the system is developed with periodic boundary condition based on the Crank–Nicolson/leap-frog methods in time and the Fourier spectral method in space. Moreover, it is shown that the scheme preserves simultaneously mass and energy conservation laws. Second, we analyze stability and convergence of the numerical scheme. Last, the numerical experiments are given, and the results show the correctness of theoretical results and the efficiency of the conservative scheme.
Utility of early, short psychological care for women who experience early miscarriage: protocol for the randomized, controlled MisTher trial
Authors: Barbe Coralie , Boiteux-Chabrier Marie , Charillon Emilie , Bouazzi Leïla , Maheas Chloe , Merabet Fadela , Graesslin Olivier , Auer Julie , Hammami Sabrina , Rolland Anne-Catherine , Hurtaud Aline 
Published: November 2023

Abstract
Background
Around one in ten women will have a miscarriage in their lifetime. Miscarriage is often considered a trivial event by caregivers, but it is associated with a high burden of psychological morbidity, especially during the first 6 months. There is no validated psychological management strategy for women who have had a miscarriage. The MisTher study aims to evaluate the utility of early, short psychological care for women who have had early miscarriage, in terms of anxiety, depression and post-traumatic stress disorder.

Methods
This is a prospective, multicenter, randomized, controlled, superiority study. In total, 932 women who have experienced early miscarriage (spontaneous interruption of pregnancy prior to 14 weeks of gestation) will be randomly assigned to either the intervention or the control group. The intervention consists of 4 teleconsultations of 45 min with a psychologist. All women, regardless of their allocated group, will be encouraged to seek an early consultation with a general practitioner or midwife. The primary endpoint will be anxiety at 3 months after randomization evaluated using State Trait Anxiety Inventory. The secondary endpoints will be anxiety at 6 months evaluated using State Trait Anxiety Inventory, depression at 3 and 6 months evaluated with the Beck Depression Inventory, and post-traumatic stress disorder at 3 and 6 months, evaluated using the Posttraumatic stress disorder Checklist Scale.

Discussion
This project will validate the importance of early psychological management, based on primary care and accessible to most women, via teleconsultation, in reducing the frequency of psychological disorders after early miscarriage. Our results should provide a basis for new recommendations for the management of women who have experienced miscarriage, notably by recommending the involvement of trained psychologists in the management pathway for these women.

Trial registration
The trial is registered with ClinicalTrials.gov: NCT05653414. December 15th, 2022.
The comparison of dispersal rate between invasive and native species varied by plant life form and functional traits
Authors: Zhang Bo , Hastings Alan , Grosholz Edwin D. , Zhai Lu 
Published: November 2023

AbstractA long dispersal distance is widely used to indicate high invasiveness, but it ignores the temporal dimensions of plant invasion. Faster dispersal rates (= distance/time) of invasive species than native ones have been widely used in modeling species invasion and planning control management. However, the comparison of dispersal rate between invasive and native plants, particularly for dispersal on a local or landscape scale, has not been tested with a comprehensive dataset. Moreover, both the effects of plant functional traits on the dispersal rate and variation in the functional-trait effects between invasive and native plants remain elusive. Compiling studies from 30 countries globally, we compared seed dispersal rates (km/year) on a local or landscape scale between 64 observations of invasive and 78 observations of native plants given effects of plant life forms, disturbance levels, and measurement methods. Furthermore, we compared the effects of functional traits on dispersal rate between invasive and native species. We found that: (1) Trait values were similar between the invasive and native plants except for the greater height of woody native plants than woody invasive ones; (2) Compared within the same plant life form, the faster dispersal rates of invasive species were found in herbaceous plants, not in woody plants, and disturbance level and measurement methods did not affect the rate comparison; (3) Plant height and seed length had significant effects on dispersal rates of both invasive and native plants, but the effect of leaf dry matter content (LDMC) was only significant on herbaceous invasive plants. The comparison of dispersal rate between invasive and native plants varied by plant life form. The convergent values but divergent dispersal effects of plant traits between invasive and native species suggest that the trait effects on invasiveness could be better understood by trait association with key factors in invasiveness, e.g., dispersal rate, than the direct trait comparison between invasive and native plants.
The ligamentum mucosum’s potential as a preventative structure in the development of knee osteoarthritis
Authors: Martin Alexandra , Liu Kenneth , Alimohammadi Majid 
Published: November 2023

AbstractPurposeThis paper aimed to identify whether the presence, type, and/or morphology of the ligamentum mucosum could play a role in the development of knee osteoarthritis. Since its microscopic structure is alike that of other knee ligaments, it was hypothesized that its presence could facilitate knee motion and stability, thus preventing or reducing the extent of knee osteoarthritis.MethodsThirty three cadavers (a total of 51 knees) were dissected. The ligamentum mucosum, if present, was measured with a digital caliber and a measuring tape in terms of length, width, and thickness. Knee osteoarthritis was assessed in six regions of the knee. The OuterBridge Classification System (Grades 0–4) was used to visually assess the extent, in addition to probing the area. Osteoarthritis was deemed present if the grade was 2 or greater.ResultsThe presence of the ligament was associated with a lower mean osteoarthritis level in the trochlear groove and lateral tibial plateau regions (p < 0.001 and p = 0.013, respectively). Overall osteoarthritis of the knee was also present at varying levels for each type of the ligamentum mucosum (p < 0.001). The patella and the medial condyle had the greatest levels of osteoarthritis, while the medial and lateral tibial plateaus had the lowest levels.ConclusionThe presence of the ligamentum mucosum is linked with decreased osteoarthritis in the trochlear groove region. In addition, both the absent ligament and its classification as a vertical septum are associated with increased knee osteoarthritis.Level of evidenceFive.
Construction and optimization of a biocatalytic route for the synthesis of neomenthylamine from menthone
Authors: Zhu Hui-Jue , Pan Jiang , Li Chun-Xiu , Chen Fei-Fei , Xu Jian-He 
Published: November 2023

Abstract(+)-Neomenthylamine is an important industrial precursor used to synthesize high value-added chemicals. Here, we report a novel biocatalytic route to synthesize (+)-neomenthylamine by amination of readily available (−)-menthone substrate using ω-transaminase. By screening a panel of ω-transaminases, an ω-transaminase from Vibrio fluvialis JS17 was identified with considerable amination activity to (−)-menthone, and then characterization of enzymatic properties was conducted for the enzyme. Under optimized conditions, 10 mM (−)-menthone was transformed in a mild aqueous phase with 4.7 mM product yielded in 24 h. The biocatalytic route using inexpensive starting materials (ketone substrate and amino donor) and mild reaction conditions represents an easy and green approach for (+)-neomenthylamine synthesis. This method underscores the potential of biocatalysts in the synthesis of unnatural terpenoid amine derivatives.
Graphical Abstract
Sub-optimal maternal gestational gain is associated with shorter leukocyte telomere length at birth in a predominantly Latinx cohort of newborns
Authors: Prasad Apurva , Lin Jue , Jelliffe-Pawlowski Laura , Coleman-Phox Kimberley , Rand Larry , Wojcicki Janet M 
Published: November 2023

Abstract
Objective
To assess in utero exposures associated with leukocyte telomere length (LTL) at birth and maternal LTL in a primarily Latinx birth cohort.

Study design
Mothers and newborns were recruited postnatally before 24 h of life. Newborn LTL was collected via heelstick at birth and maternal LTL was collected postnatally. LTL was determined by quantitative PCR. Using a longitudinal design, we evaluated associations between neonatal and maternal LTL and appropriate maternal gestational gain as indicated by the American College of Obstetrics and Gynecology (ACOG).

Result
Mean infant LTL was 2.02 ± 0.30 T/S (n = 386) and maternal LTL was 1.54 ± 0.26 T/S (n = 58). Independent risk factors for shorter LTL at birth included longer gestational duration (Coeff:-0.03, 95%CI: -0.05—0.01;p < 0.01) and maternal gestational weight gain below ACOG recommendations (Coeff:-0.10, 95%CI: -0.18 – -0.02; p = 0.01).

Conclusion
Gestational weight gain below ACOG recommendations may adversely impact neonatal health in Latinx infants as indicated by shorter LTL at birth.
Understanding the use of artificial intelligence for implant analysis in total joint arthroplasty: a systematic review
Authors: Shah Aakash K. , Lavu Monish S. , Hecht Christian J. , Burkhart Robert J. , Kamath Atul F. 
Published: November 2023

Abstract
Introduction
In recent years, there has been a significant increase in the development of artificial intelligence (AI) algorithms aimed at reviewing radiographs after total joint arthroplasty (TJA). This disruptive technology is particularly promising in the context of preoperative planning for revision TJA. Yet, the efficacy of AI algorithms regarding TJA implant analysis has not been examined comprehensively.

Methods
PubMed, EBSCO, and Google Scholar electronic databases were utilized to identify all studies evaluating AI algorithms related to TJA implant analysis between 1 January 2000, and 27 February 2023 (PROSPERO study protocol registration: CRD42023403497). The mean methodological index for non-randomized studies score was 20.4 ± 0.6. We reported the accuracy, sensitivity, specificity, positive predictive value, and area under the curve (AUC) for the performance of each outcome measure.

Results
Our initial search yielded 374 articles, and a total of 20 studies with three main use cases were included. Sixteen studies analyzed implant identification, two addressed implant failure, and two addressed implant measurements. Each use case had a median AUC and accuracy above 0.90 and 90%, respectively, indicative of a well-performing AI algorithm. Most studies failed to include explainability methods and conduct external validity testing.

Conclusion
These findings highlight the promising role of AI in recognizing implants in TJA. Preliminary studies have shown strong performance in implant identification, implant failure, and accurately measuring implant dimensions. Future research should follow a standardized guideline to develop and train models and place a strong emphasis on transparency and clarity in reporting results.

Level of Evidence
Level III.
Letrozole plus misoprostol versus misoprostol alone in the induction of anembryonic missed abortion: a randomized controlled trial conducted in Upper Egypt
Authors: Tadros Ramy Heshmat Zekry , Fouda Usama , Zaki Sherif Sameh , Abdalla Marwa 
Published: November 2023

Abstract
Background
A missed abortion is a spontaneous abortion in which the embryo or fetus has already died but has remained in the uterus for days or weeks with a closed cervical ostium. Termination of pregnancy could be achieved either by surgical evacuation or medical or expectant management. Letrozole is a third-generation aromatase inhibitor. According to many recent studies, letrozole given for three days before the administration of misoprostol was more effective than misoprostol alone at inducing abortion. Our study compared the efficacy of various letrozole regimens (10 mg/d for three days and a single dose of 20 mg) combined with misoprostol vs. misoprostol alone in inducing abortion. To find the most efficient regimen of letrozole administered before misoprostol to induce an abortion.

Methodology
This prospective, parallel, three-arm, single-blinded, allocation-concealed randomized controlled trial was conducted in Sohag Teaching Hospital in Upper Egypt. We randomly divided 105 patients with anembryonic missed abortion (up to 63 days gestation) with no history of medical disorders or a history of allergies to misoprostol or letrozole into three equal groups (a single-dose letrozole group, a multiple-dose letrozole group, and a misoprostol-only group). The complete abortion rate, incomplete abortion rate, failure to abort rate, and induction-to-abortion interval were all collected. All statistical calculations were performed using the computer program SPSS (Statistical Package for the Social Science, SPSS Inc., Chicago, IL, USA).

Results
The complete abortion rate was significantly higher in the single-dose letrozole and multiple-dose letrozole groups than in the misoprostol group (p values = 0.0455 and 0.001, respectively). On the other hand, there was no significant difference in the complete abortion rate between the single-dose group and the multiple-dose letrozole group (p-value = 0.1713). The time to complete abortion was significantly shorter in the single-dose and multiple-dose letrozole groups than in the misoprostol group (p values = 0.0036 and 0.0049, respectively). On the other hand, there was no significant difference in the time to complete abortion between the single-dose letrozole group and the multiple-dose letrozole group (P = 0.532).

Conclusion
Single- and multiple-dose letrozole regimens followed by misoprostol had a higher rate and a shorter time to complete abortion than misoprostol alone.

Trial registration
The trial is registered at gov with the name “letrozole and abortion” and the identifier “NCT05198050”. The date of registration was April 1, 2022, registered prospectively. URL: https://register.clinicaltrials.gov/prs/app/action/ViewOrUnrelease?uid=U0004GED&ts=25&sid=S000BPDQ&cx=43mobl.
Complexities in liver biopsy: the role of navigation and fusion imaging
Authors: Mathew Serah Jessy , Nayak Anchal , Dash Sagnika , Dakua Sarada Prasad 
Published: November 2023

AbstractLiver biopsy is crucial to know if the tumor is benign or malignant. This paper has reviewed the literature clinically shedding lights on the present biopsy procedure, requirements, and potential challenges. This study has emphasized the role of navigation during liver biopsy. It has discussed the various imaging modalities used for biopsy. The potential limitations of imaging modalities have been discussed in detail. It is found that liver biopsy could be effective when fusion imaging is used instead of a single imaging modality.