bluuebunny/crossref_metadata_2025_split huggingface dataset:
Responsiveness at the Local Level: Building VET Systems to Advance Communities
Authors: Billett Stephen 
Published: February 2025

ABSTRACTTo advance the social and economic goals of the communities that vocational education and training (VET) serves requires more than being responsive to governmental and industry needs. While necessary to a degree, being responsive to such needs is not a sufficient goal to which VET systems should be directed, enacted and judged. They also need to: (i) be responsive to the communities served by those systems and (ii) play a role in bringing about change in those communities through supporting innovations, extending existing economic activities and building capacities at the local level. Given the scope of these roles, VET, perhaps more than any other educational sector, requires effective localised social, administrative and educational infrastructures to achieve such outcomes. Social infrastructure includes partnerships that support work placements, work experiences, employment opportunities and articulate local enterprises' requirements. Administrative infrastructure includes the intentional organisation and enactment of vocational educational provisions and their certification. Educational infrastructure includes the provision and alignment of expertise and resources to achieve these outcomes, including those of teachers and the ability to extend their efforts beyond the educational institution. These forms of local infrastructure are proposed as being essential for achieving five contemporary purposes of VET: (i) engaging young people with VET; (ii) assisting them in identifying occupations to which they are suited; (iii) preparing them for occupations; (iv) continuing education across working life to meet changing needs and goals and (v) aligning workplace innovations with workers' learning. In advancing that proposal, this paper draws on the reviews of literature and findings from a three‐decade‐long programme of research in VET provisions in countries with both developed and developing economies, to make a case for VET going beyond responsiveness to meet local communities' needs, advance their capacities and extend their social and economic potentials.
Wood Anatomical and Hydraulic Traits of Tamarix Species Across a Large Eurasian Gradient Show a Stronger Climatic Than Phylogenetic Signal
Authors: Akhmedov Akbar , Bobokandov Nodirjon , Krehenwinkel Henrik , Rzepecki Andreas , Klein Tamir , Villar Jose L. , Thomas Frank M. 
Published: February 2025

ABSTRACTAimTamarisk (Tamarix) is the predominant but taxonomically complex genus of the Tamaricaceae. The morphologically similar shrub or tree species grow in arid, salt‐influenced habitats of Eurasia and Africa. We sampled woody shoots of seven species at the eastern margin (NW China; T. ramosissima) and the centre (Uzbekistan; T. hispida) of the Indo‐Turanian region as well as at the eastern (Israel; T. aphylla, T. negevensis, T. nilotica) and western margin (southern Spain; T. boveana, T. gallica) of the Mediterranean region, the two diversity centres of the genus. To explore the drivers of intrageneric trait variability, we investigated whether differences in the anatomical‐hydraulic wood traits among the species are related to climate or phylogeny.LocationEurasia.TaxonTamarix L. (Tamaricaceae).MethodsWe determined features of wood anatomy and calculated the hydraulic conductivity (kt) and the water potential at 50% loss of hydraulic conductance (P50). We related these traits to climate variables and to the phylogenetic distances among the species.ResultsTamarix nilotica, T. negevensis and T. ramosissima exhibited large conduit areas, large hydraulic diameters and high kt, whereas the Spanish species T. gallica and T. boveana displayed high wood densities and a small fraction of large conduits. The phylogenetically distant species T. aphylla and T. hispida took intermediate positions. Tamarix ramosissima, which grows in regions with cold winters and hot‐dry summers, exhibited the most negative P50 values, indicative of a low susceptibility to a failure of the water‐conducting system. Trait differences among the species were unrelated to the species' phylogenetic relatedness but correlated with climate variables.Main ConclusionsDespite strong similarity in morphology and habitat preferences, Tamarix species displayed significant differences in their anatomical‐hydraulic traits. These differences were related to climate conditions rather than phylogeny and are indicative of specific local adaptations to environmental conditions.
Tiny Hunters Along the Alpine Fault: Integrative Phylobiogeography Demonstrates High Geographic Structure in a Forest‐Dwelling Aotearoa Harvester Genus (Arachnida, Triaenonychidae: Algidia)
Authors: Frigyik Ella , Baker Caitlin M. , Sirvid Phil , Derkarabetian Shahan , Giribet Gonzalo 
Published: February 2025

ABSTRACTAimThe archipelago of Aotearoa displays both high biodiversity and a dynamic geologic history, shaped by constantly shifting coastlines and the dramatic effects of glacial cycling on forest cover across the islands. This geographic history has important implications for the evolution of dispersal‐limited forest‐dwelling arthropods, such as Opiliones, which can help us reconstruct key past biogeographic events. In this study, we shed light on the evolutionary history of the triaenonychid genus Algidia Hogg, 1920.LocationThe archipelago of Aotearoa|New Zealand.Time PeriodLate Cretaceous to the present‐day, with particular focus on events in the Oligocene onwards.Major Taxa StudiedAlgidia, Triaenonychidae, Opiliones, Arachnida.MethodsWe utilise an integrative phylobiogeographic approach, incorporating target enrichment sequence capture of ultraconserved elements, divergence dating, species delimitation and ecological niche modeling.ResultsOur genomic data in conjunction with divergence dating find evidence of high geographic structure and the influence of multiple key geologic events in the natural history of Aotearoa, including the origination and continuation of the Alpine Fault, marine transgression during the Oligocene and cycles of glaciation and orogeny that characterised the Pliocene and Pleistocene on the islands. Our results recover 10 putative species, including four that are undescribed. Paleoclimate modelling reflects geographic changes to Aotearoa's coastline which potentially underpin the modern distributions of Algidia, including land bridges in place of the current marine straits Raukawa Moana|Cook Strait and Te Ara‐a‐Kiwa|Foveaux Strait.Main ConclusionsThe phylogeny of Algidia indicates consistent northwards expansion, with the earliest diverging clade, A. homerica, located in Rakiura and southern Te Waipounamu, and subsequently diverging clades moving steadily northwards in their geographic distributions. Diversification of Algidia predates the Oligocene Marine Transgression, lending support to the now well‐established hypothesis that Aotearoa was not fully submerged during the Oligocene. The Alpine Fault seems to be an important feature explaining cladogenesis and diverging populations, including for species found across Raukawa Moana. However, other phenomena, including glaciation, orogeny or continental shifting, are also important explanatory factors in species distributions across Aotearoa.
CTSL Promotes Autophagy in Laryngeal Cancer Through the IL6‐JAK‐STAT3 Signalling Pathway
Authors: Wang Xueying , Wang Junrong , Wang Lei , Song Ming , Meng Hongxue , Guo Erliang , Miao Susheng 
Published: February 2025

ABSTRACTCathepsin L (CTSL) is an important oncogene. However, its mechanism of action in laryngeal cancer is still unclear. This study aims to explore the role of CTSL in laryngeal cancer and its clinical significance. Conducting bioinformatics analysis utilising the Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) database. Performing CCK8 analysis, Western blotting, qRT‐PCR, wound healing assay and transwell invasion assay. Additionally, conducting immunoprecipitation experiments and immunohistochemical staining to investigate the impact of CTSL on cell proliferation, autophagy and related signalling pathways. We observed a significant upregulation of CTSL in laryngeal cancer tissues, and its elevated levels are indicative of poor prognosis in laryngeal cancer patients. The proliferation of laryngeal cancer cells relies on the expression of CTSL, with overexpression of this gene enhancing the proliferative capacity of these cells. Concurrently, CTSL is closely associated with the autophagic levels in laryngeal cancer cells. During the autophagic process mediated by CTSL, the IL6‐JAK‐STAT3 signalling pathway is activated, suggesting that CTSL promotes autophagy through the IL6‐JAK‐STAT3 pathway. Considering the correlation between CTSL and autophagy, we developed and validated a multi‐gene signature. The risk score derived from this signature demonstrates significant potential in predicting various aspects. We found that CTSL upregulates autophagy in laryngeal cancer cells by activating the IL6‐JAK‐STAT3 signalling pathway. By taking into account the autophagy‐regulating role of CTSL, the clinical predictive ability of CTSL in HNSC can be enhanced.
Sleep regularity in adolescents: Associations with sleep, rest–activity indices, and prospective substance use and depression
Authors: Walsh Rachel F. L. , Maddox Mackenzie A. , Smith Logan T. , Olino Thomas , Zee Phyllis C. , Nusslock Robin , Alloy Lauren B. 
Published: February 2025

SummarySleep is important for adolescent development and has been linked to a variety of mental health difficulties. New research suggests that sleep irregularity may be more clinically relevant than relying on mean values. This study aimed to (1) quantify the relationship between the sleep regularity index (SRI), a novel parameter reflecting the probability that an individual will be in the same state [asleep or awake] 24 h apart, and other actigraphy‐derived sleep and rest–activity metrics, (2) evaluate concurrent and prospective associations between the SRI and mental health symptoms (depressive symptoms and substance use), and (3) test sex as a moderator. At baseline, a community‐based sample of adolescents (n = 295, 56.27% female, 58.98% White, age = 15.44) wore an actiwatch for 1 week to measure rest–activity rhythms and sleep–wake cycles. Actigraphy data were used to derive sleep metrics, SRI, and nonparametric rest–activity indices. Adolescents also completed self‐report measures of substance use and depression at baseline and at 6‐month follow‐up. Partial correlations indicated that individuals with more sleep irregularity had less interdaily stability, less robust relative amplitude, lower sleep efficiency, shorter total sleep time, longer sleep onset latency, more social jetlag, and a later sleep midpoint. Regression analyses revealed that sleep irregularity was associated with a lifetime history of substance use, first‐onset substance use, and a prospective increase in depressive symptoms, even after accounting for sleep duration and subjective sleep disturbances. The sleep regularity index may be a potential modifiable risk factor for enhancing adolescent mental health.
Relationship Between Preoperative Fasting Duration and Anxiety Levels With Postoperative Pain, Nausea‐Vomiting, and Sleep Quality in Orthopedic Surgical Patients: A Cross‐Sectional Study
Authors: Kirtil İnci , Aydin Ebrar 
Published: February 2025

ABSTRACTThis study is a cross‐sectional study aimed to determine the association between preoperative fasting time and anxiety level and early postoperative pain intensity, nausea‐vomiting severity, and sleep quality in patients undergoing orthopedic surgery. The study was conducted between March and June 2024 and completed with 188 patients. In the study, patient information form, Amsterdam Preoperative Anxiety and Information Scale, and Richards‐Campbell Sleep Questionnaire were used. Correlation analysis indicated a significant negative correlation between postoperative sleep quality and pain intensity. There was a significant positive correlation between preoperative anxiety level and preoperative solid food fasting and fluid fasting times. According to the multiple linear regression model, preoperative anxiety level was found to have a significant effect on pain severity, and pain severity at the postoperative 24th hour significantly affected postoperative sleep quality. Adjusting patients' fasting durations prior to surgical interventions according to evidence‐based guideline recommendations and monitoring anxiety closely are simple, practical, and safe measures that could alleviate postoperative pain and nausea‐vomiting and improve sleep quality, potentially enhancing outcomes, comfort, and the quality of care.
Construction of Internet Plus Wound Care Service Quality Evaluation System Based on Donabedian and SERVQUAL Models—A Delphi‐Analytic Hierarchy Process Study
Authors: Wei Li , Zhang Xin , Li Huimin , Wang Yanmei , Lu Minghui , Chai Qianwen 
Published: February 2025

ABSTRACTThe objective of this study was to develop an Internet Plus wound care service quality evaluation system within the context of digital and intelligent medical care services. We utilized the Donabedian and SERVQUAL models as the theoretical framework, establishing an initial evaluation system through a literature review and semi‐structured interviews. This evaluation system was refined through two rounds of Delphi expert consultations, and the weights of each level of indicators were determined using the Analytic Hierarchy Process (AHP). The statistical results indicated a return rate of 100%, with authority coefficients of the experts at 0.898 and 0.906, respectively. The experts' opinions were relatively concentrated and consistent. After two rounds of consultations, the importance values of indicators exceeded 4.5 points, the variation coefficient was below 0.25, and the Kendall coordination coefficients for secondary and tertiary indicators were 0.163 and 0.131, respectively, all with p < 0.001. The final indicator system comprised 3 primary indicators, 10 secondary indicators, and 59 tertiary indicators, demonstrating strong scientific validity and reliability.
Long‐Term Survival in Children Following Heart Transplantation
Authors: Hayes Emily A. , Koehl Devin , Cantor Ryan , Fisher Lauren A. , Azeka Estela , Mokshagundam Deepa , Asante‐Korang Alfred , Rusconi Paolo , O'Connor Matthew J. , Nandi Deipanjan , Kirklin James K. , Boyle Gerard J. 
Published: February 2025

ABSTRACTBackgroundShort‐term outcomes following heart transplantation in children have improved, but comparable improvements in long‐term survival continues to have barriers. We sought to investigate long‐term outcomes following heart transplantation and to identify protective and risk factors associated with long‐term survival in children.MethodsThe Pediatric Heart Transplant Society (PHTS) database was queried for heart transplant recipients from 1993 to 2010 who were ≤ 10 years of age at time of transplant. Patients with conditional graft survival > 3 years and at ≥ 10 years were analyzed. Survival and time‐to‐event were compared using the Kaplan–Meier method with a log‐rank test for significance. Factors associated with graft loss were identified using Cox proportional hazard modeling.ResultsThere were 1610 patients ≤ 10 year of age who were transplanted between 1993 and 2010 with conditional survival to 3 years post‐transplant. Of those patients, there were 1170 with conditional survival to 10 years post‐transplant. Patients < 1 year at transplant had improved survival compared to other age groups. Risk factors for graft loss after 3 years post‐transplant were malignancy, rejection, cardiac allograft vasculopathy (CAV), age, congenital heart disease, female sex, and Black race (p value for all < 0.05).ConclusionsHeart transplantation remains an effective therapy in children with a growing number of long‐term survivors. Risk factors for mortality in patients ≤ 10 years of age at transplant with conditional survival to 3 years post‐transplant include CAV, rejection, malignancy, female sex, and Black race. Further studies are needed to understand the social and biologic causes of racial and sex disparities in pediatric transplant patients.
Unravelling Candidate Genes Associated With Blast Disease Resistance in an Elite Green Super Rice Varietal Panel Using Genome‐Wide Association Study
Authors: Saxena Harshita , Murugaiyan Varunseelan , Ghimire Bikash , Robiso Christian John , De Asis Erik Jon , Yanoria Mary Jeanie , Raymer Paul L. , Bahri Bochra Amina , Ali Jauhar 
Published: February 2025

ABSTRACTRice blast disease, caused by the filamentous fungus Magnaporthe oryzae, significantly threatens global rice crops leading to yield losses worldwide. Given that existing resistance genes often fail to control rice blast due to the evolution of new virulent strains, identifying novel genes using modern breeding tools to enhance partial resistance is crucial for developing more durable and effective control measures. Here, we used a genome‐wide association study (GWAS) with 2698 high‐throughput single‐nucleotide polymorphism (SNP) markers to analyse 134 rice cultivars from the Green Super Rice breeding programme against two virulent rice blast isolates, M101‐1‐2‐9‐1 (M101) and M64‐1‐3‐9‐1 (M64). GWAS identified 12 potential quantitative trait loci (QTLs) for blast resistance: five against M101 on chromosomes 1, 2, 3, 4 and 7 and seven against M64 on chromosomes 1, 4, 5, 6 and 12, with no QTL in common against both. Notably, qM101_2 is within the 500 kb linkage disequilibrium (LD) block containing the known resistance gene Pib on chromosome 2, and qM64_12.1 and qM64_12.2 are within the Pi‐ta and Ptr gene cluster on chromosome 12. The remaining nine QTLs represented novel blast disease resistance sources. The identified candidate genes, including those encoding nucleotide‐binding site leucine‐rich repeats domains, protein kinases, resistance gene analogues and pathogenesis‐related proteins, may serve as a foundation for further studies to explore their potential role in enhancing disease resistance in rice.
Systematics and evolutionary history of raft and nursery‐web spiders (Araneae: Dolomedidae and Pisauridae)
Authors: Yu Kuang‐Ping , Cheng Ren‐Chung , Haddad Charles R. , Tanikawa Akio , Pett Brogan L. , Piacentini Luis N. , Yip Ho Yin , Suzuki Yuya , Henrard Arnaud , Painting Christina J. , Vink Cor J. , Hebets Eileen A. , Harvey Mark S. , Kuntner Matjaž 
Published: February 2025

AbstractPisauridae are a global and heterogeneous assemblage of spider genera with diverse morphologies and lifestyles. So far, the monophyly of Pisauridae and the inclusion of fishing spiders (Dolomedes) in this family have not been thoroughly tested. Here, we amend the systematics and classification of these lineages within a UCE phylogenomic framework and through a detailed morphological reappraisal. For estimations of their evolutionary age, we perform and compare outcomes from two divergence estimation approaches, an a posteriori likelihood, and an a priori Bayesian. Phylogenies reject the monophyly of both Pisauridae and Dolomedes: (1) Focal Clade I groups true Pisauridae genera including Pisaura; (2) Focal Clade II contains Blandinia and is sister to Trechaleidae and Lycosidae; (3) Focal Clade III groups Dolomedes, Megadolomedes, and Ornodolomedes, and is sister to Blandinia, Trechaleidae, and Lycosidae. We therefore propose to delimit Pisauridae by removing Dolomedidae rank resurrected (including Dolomedes, Bradystichus, Megadolomedes, Caledomedes, Mangromedes, Ornodolomedes, and Tasmomedes) and Blandinia incertae sedis. Likelihood and Bayesian time calibration approaches yield comparable divergence estimations: Pisauridae origin is estimated at 29–40 Ma; Blandinia 21–34 Ma; Dolomedidae 10–17 Ma; Dolomedes 9–16 Ma. Reconstructions suggest that the evolution of terrestrial and web‐building lifestyles from semi‐aquatic ancestors in Pisauridae coincided with cooling and drying climates during the mid‐Miocene, but this was not the case in the few recent cases of terrestrialization in Dolomedes species. This historic reconstruction illustrates how climatic changes, or rapid radiation, can drive lifestyle diversification.
Elevated CA15‐3 Levels in Myeloid Disorders: Clinicopathological Correlation
Authors: Saharti Samah , Qari Mohamad 
Published: February 2025

Background: Serum tumor markers are beneficial to patients with advanced cancer because they help in early diagnosis, determining prognosis, predicting response to certain medications, and monitoring therapy. The prognostic value of cancer antigen 15‐3 (CA15‐3) serum‐level changes in breast cancer is very well established. The main cause behind the elevation of CA 15‐3 is metastatic breast carcinoma. In this study, we will run a record review to study comprehensively the clinical association between chronic myeloid disorders and CA15‐3 elevation.Method: In this retrospective study, we run CA15‐3 on 106 patients with different myeloid disorders diagnosed between 2008 and 2021 from several tertiary care centers in Saudi Arabia, Jeddah, majority of which (38 cases) were diagnosed with chronic myeloid leukemia (35.8%). Others had essential thrombocytosis (22.6%), chronic myeloproliferative neoplasm (20.8%), myelofibrosis (7.5%), acute myeloid leukemia (4.7%), myelodysplastic syndrome (4.7%), and polycythemia vera (3.8%).Result: An increase in CA15‐3 was seen in 50% of all cases. In particular, polycythemia vera showed an elevation in 100% of the cases (4 out of 4). Second in expressivity is myelodysplastic syndrome (80%, 4 out of 5 cases). Close in value is myelofibrosis (75%, 6 out of 8 cases). The least association is noted between CA15‐3 and essential thrombocytosis (16.7%, 4 out of 24 cases).Conclusion: When CA15‐3 levels are considered during the time of myeloid disorder diagnosis, it shows an independent predictive value. More research is needed to determine the utility of these serum biomarkers in myeloid disorders decision‐making. This is a thorough case series of chronic myeloid disorders inclusive of all myeloproliferative neoplasms besides myelodysplastic syndrome describing the association with CA15‐3.
Wernicke Encephalopathy Related to Hyperemesis Gravidarum: A Retrospective Study of 12 Cases
Authors: Abouelbaqua Kaouthar , Rebahi Houssam , Louhab Nisrine , Kissani Najib , El Adib Ahmed Rhassane 
Published: February 2025

Objective: Wernicke encephalopathy (WE) related to hyperemesis gravidarum (HG) is a devastating neuropsychiatric syndrome that remains frequently undiagnosed in pregnant women. Although many cases have been published, more studies are required to establish guidelines for the early detection and treatment of this condition.Material and Methods: We conducted a retrospective study to analyze the available data concerning 12 cases of WE complicating HG in the Mother and Child Hospital’s Obstetric Intensive Care Unit, belonging to Mohamed VI University Hospital of Marrakesh.Results: Twelve out of 76 HG admitted cases developed WE. Pregnant WE patients became depleted after 11 weeks of vomiting at median gestational weeks of 16.2. They had a severe weight loss of more than 5% of their body and had all presented prodromal signs of WE before the actual onset of the clinical triad. WE diagnosis was clinically made based on Caine’s operational criteria as they allow early identification of the disease. A good tool that could also aid diagnosis is an encephalic MRI; however, it should not delay treatment with prompt administration of high doses of thiamin of > 500 mg/day. Chronic sequelae in this study occurred in 45.5% and death in one case.Conclusion: In HG, thiamin rapidly depletes which can lead to WE with adverse outcomes for the mother and fetus. Therefore, physicians must be vigilant in detecting early signs of WE to promptly provide a high dose of thiamin with targeted multimodal therapies as this could be lifesaving.