bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations between diet and disease activity in ulcerative colitis patients using a novel method of data analysis
Authors: Magee Elizabeth A , Edmond Laurie M , Tasker Shiona M , Kong San Choon , Curno Richard , Cummings John H 
Published: February 2005

Abstract
Background
The relapsing nature and varying geographical prevalence of ulcerative colitis (UC) implicates environmental factors such as diet in its aetiology.

Methods
In order to determine which foods might be related to disease activity in UC a new method of dietary analysis was developed and applied. Eighty-one UC patients were recruited at all stages of the disease process. Following completion of a 7 d diet diary, clinical assessment including a sigmoidoscopic examination (scale 0 (normal mucosa) to 6 (very active disease)) was conducted. Food weights for each person were adjusted (divided) by the person's calorific intake for the week. Each food consumed was given a food sigmoidoscopy score (FSS) calculated by summing the products of the (adjusted) weight of food consumed and sigmoidoscopy score for each patient and occurrence of food and dividing by the total (adjusted) weight of the food consumed by all 81 patients. Thus, foods eaten in large quantities by patients with very active disease have high FSSs and vice versa. Foods consumed by <10 people or weighing <1 kg for the whole group were excluded, leaving 75 foods.

Results
High FSS foods were characterized by high levels of the anti-thiamin additive sulfite (Mann-Whitney, p < 0.001), i.e. bitter, white wine, burgers, soft drinks from concentrates, sausages, lager and red wine. Caffeine also has anti-thiamin properties and decaffeinated coffee was associated with a better clinical state than the caffeine containing version. Beneficial foods (average intake per week) included pork (210 g), breakfast cereals (200 g), lettuce (110 g), apples and pears (390 g), milk (1250 ml), melon (350 g), bananas (350 g), bacon (120 g), beef and beef products (500 g), tomatoes (240 g), soup (700 g), citrus fruits (300 g), fish (290 g), yogurt (410 g), cheese (110 g), potatoes (710 g) and legumes (120 g).

Conclusions
The dietary analysis method described provides a new tool for establishing relationships between diet and disease and indicates a potentially therapeutic diet for UC.
Long-term survival from gastrocolic fistula secondary to adenocarcinoma of the transverse colon
Authors: Forshaw Matthew J , Dastur Jamasp K , Murali Kothandaraman , Parker Michael C 
Published: February 2005

Abstract
Background
Gastrocolic fistula is a rare presentation of both benign and malignant diseases of the gastrointestinal tract. Malignant gastrocolic fistula is most commonly associated with adenocarcinoma of the transverse colon in the Western World. Despite radical approaches to treatment, long-term survival is rarely documented.

Case presentation
We report a case of a 24-year-old woman who presented with the classic triad of symptoms associated with gastrocolic fistula. Radical en-bloc surgery and adjuvant chemotherapy were performed. She is still alive ten years after treatment.

Conclusions
Gastrocolic fistula is an uncommon presentation of adenocarcinoma of the transverse colon. Radical en-bloc surgery with adjuvant chemotherapy may occasionally produce long-term survival.
LDL receptor expression on T lymphocytes in old patients with Down syndrome
Authors: Corsi Massimiliano M , Malavazos Alexis E , Passoni Daniele , Licastro Federico 
Published: February 2005

Abstract

Background
In Down syndrome patients several metabolic abnormalities have been reported, some involving the lipid metabolism. The level of LDL in plasma is the major determinant of the risk of vascular disease. There appear to be no studies on the LDL receptor in Down syndrome patients.

Methods
Flow cytometric methods for measuring the LDL receptor in peripheral blood mononuclear cells (PBMC) can identify patients with hypercholesterolemia. We applied this method in 19 old patients with Down syndrome and 23 healthy controls.

Results
Down syndrome patients had high levels of triglycerides and low levels of HDL, and high levels of CRP. We also found a down-regulation of LDL receptor expression.

Conclusions
Down syndrome patients show no increase in the frequency of cardiovascular disease. The low incidence in cardiovascular disease despite the low level of HDL, high levels of CRP and reduction of LDL receptor expression lead to the conclusion that either these are not risk factors in these patients or that other risks factors – not yet identified – are considerably lower.
Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
Authors: Radonić Aleksandar , Thulke Stefanie , Bae Hi-Gung , Müller Marcel A , Siegert Wolfgang , Nitsche Andreas 
Published: February 2005

Abstract
Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.
Randomized Trial of Two Intravenous Schedules of the Topoisomerase I Inhibitor Liposomal Lurtotecan in Women With Relapsed Epithelial Ovarian Cancer: A Trial of the National Cancer Institute of Canada Clinical Trials Group
Authors: Dark Graham G. , Calvert A. Hilary , Grimshaw Robert , Poole Christopher , Swenerton Ken , Kaye Stan , Coleman Robert , Jayson Gordon , Le Tien , Ellard Susan , Trudeau Marc , Vasey Paul , Hamilton Marta , Cameron Terri , Barrett Emma , Walsh Wendy , McIntosh Lynn , Eisenhauer Elizabeth A. 
Published: February 2005

Purpose Liposomal lurtotecan (OSI-211) is a liposomal formulation of the water-soluble topoisomerase I inhibitor lurtotecan (GI147211), which demonstrated superior levels of activity compared with topotecan in preclinical models. We studied two schedules of OSI-211 in a randomized design in relapsed ovarian cancer to identify the more promising of the two schedules for further study. Patients and Methods Eligible patients had measurable epithelial ovarian, fallopian, or primary peritoneal cancer that was recurrent after one or two prior regimens of chemotherapy. Patients were randomly assigned to receive either arm A (OSI-211 1.8 mg/m2/d administered by 30-minute intravenous infusion on days 1, 2, and 3 every 3 weeks) or arm B (OSI-211 2.4 mg/m2/d administered by 30-minute intravenous infusion on days 1 and 8 every 3 weeks). The primary outcome measure was objective response, which was confirmed by independent radiologic review, and a pick the winner statistical design was used to identify the schedule most likely to be superior. Results Eighty-one patients were randomized between October 2000 and September 2001. The hematologic toxic effects were greater on arm A than on arm B (grade 4 neutropenia, 51% v 22%, respectively), as was febrile neutropenia (26% v 2.4%, respectively). Of the 80 eligible patients, eight patients (10%) had objective responses; six responders (15.4%; 95% CI, 6% to 30%) were in arm A and two responders (4.9%; 95% CI, 1% to 17%) were in arm B. Conclusion The OSI-211 daily for 3 days intravenous schedule met the statistical criteria to be declared the winner in terms of objective response. This schedule was also associated with more myelosuppression than the schedule of OSI-211 administered in arm B.
Phase I Clinical Study of Pertuzumab, a Novel HER Dimerization Inhibitor, in Patients With Advanced Cancer
Authors: Agus David B. , Gordon Michael S. , Taylor Charles , Natale Ronald B. , Karlan Beth , Mendelson David S. , Press Michael F. , Allison David E. , Sliwkowski Mark X. , Lieberman Gracie , Kelsey Stephen M. , Fyfe Gwen 
Published: February 2005

Purpose Pertuzumab, a recombinant humanized monoclonal antibody (2C4), binds to extracellular domain II of the HER-2 receptor and blocks its ability to dimerize with other HER receptors. Pertuzumab represents a new class of targeted therapeutics known as HER dimerization inhibitors. A clinical study was conducted to investigate safety and pharmacokinetics of pertuzumab and to perform a preliminary assessment of HER dimerization inhibition as a treatment strategy. Patients and Methods Patients with incurable, locally advanced, recurrent or metastatic solid tumors that had progressed during or after standard therapy were recruited to a dose-escalation study of pertuzumab (0.5 to 15 mg/kg) given intravenously every 3 weeks. Results Twenty-one patients received pertuzumab and 19 completed at least two cycles. Pertuzumab was well tolerated. Overall, 365 adverse events were reported and 122 considered to be possibly drug related. Of these, 116 were of grade 1 to 2 intensity. The pharmacokinetics of pertuzumab were similar to other humanized immunoglobulin G antibodies, supporting a 3-week dosing regimen. Trough plasma concentrations were in excess of target concentrations at doses greater than 5 mg/kg. Two patients, one with ovarian cancer (5.0 mg/kg) and one with pancreatic islet cell carcinoma (15.0 mg/kg), achieved a partial response. Responses were documented by Response Evaluation Criteria in Solid Tumors after 1.5 and 6 months of pertuzumab therapy, and lasted for 11 and 10 months, respectively. Stable disease lasting for more than 2.5 months (range, 2.6 to 5.5 months) was observed in six patients. Conclusion These results demonstrate that pertuzumab is well tolerated, has a pharmacokinetic profile which supports 3-week dosing, and is clinically active, suggesting that inhibition of dimerization may be an effective anticancer strategy.
Bone Response to Intermittent Parathyroid Hormone Is Altered in Mice Null for β-Arrestin2
Authors: Ferrari S. L. , Pierroz D. D. , Glatt V. , Goddard D. S. , Bianchi E. N. , Lin F. T. , Manen D. , Bouxsein M. L. 
Published: February 2005

AbstractIntermittent PTH administration increases bone turnover, resulting in net anabolic effects on bone. These effects are primarily mediated by intracellular cAMP signaling. However, the molecular mechanisms that regulate PTH activity in bone remain incompletely understood. β-Arrestin2, a G protein-coupled receptor regulatory protein, inhibits PTH-stimulated cAMP accumulation in vitro. Using β-arrestin2−/− (KO) and wild-type (WT) mice, we investigated the response to PTH in primary osteoblasts (POB) and the effects of intermittent PTH administration on bone mass and microarchitecture in vivo. Compared with that in WT mice, PTH-stimulated intracellular cAMP was increased and sustained in KO POB. Intermittent exposure of POB to PTH significantly decreased the ratio of osteoprotegerin (OPG) receptor activator of nuclear factor-κB ligand (RANKL) mRNA expression in KO POB, whereas it increased this ratio in WT POB. Total body bone mass and cortical and trabecular bone parameters were 5–10% lower in male KO mice compared with WT, and these differences were magnified upon in vivo administration of intermittent PTH (80 μg/kg·d) for 1 month. Thus, PTH significantly increased total body bone mineral content as well as vertebral trabecular bone volume and thickness in WT, but not KO mice. The anabolic response to PTH in cortical bone was also slightly more pronounced in WT than KO mice. Histomorphometry indicated that PTH prominently stimulated indexes of bone formation in both WT and KO mice, whereas it significantly increased indexes of bone resorption (i.e. osteoclast number and surface) in KO mice only. In conclusion, these results suggest that β-arrestins may specify the activity of intermittent PTH on the skeleton by limiting PTH-induced osteoclastogenesis.
Genetic Analysis of the Hypothalamic Corticotropin-Releasing Factor System
Authors: Garlow Steven J. , Boone Ericka , Li Wei , Owens Michael J. , Nemeroff Charles B. 
Published: February 2005

AbstractThe goal of this study was to use BxD recombinant inbred mice to search for genes that control the hypothalamic corticotrophin-releasing factor (CRF) system. The specific phenotype that was measured was abundance of transcripts that encode CRF, CRF receptor (Crf-R1), CRF binding protein, and arginine vasopressin (AVP) in total hypothalamic RNA. The strain distribution patterns for the transcript abundances for each target were continuously distributed, consistent with these being quantitative traits. Marker regression and interval mapping revealed associations with quantitative trait loci (QTL) for CRF transcript abundance on chromosome 1 (at 89.2 cM), chromosome 12 (between 54–58 cM), and chromosome 13 (between 26–30 cM); for Crf-R1 transcript abundance on chromosome 7 (at 1.5 cM), chromosome 12 (at 37 cM), and chromosome X (at 30 cM); for CRF binding protein transcript abundance on chromosome 7 (at 48.5 cM), chromosome 8 (at 65 cM), and chromosome 12 (at 19 cM); and for AVP transcript abundance on chromosome 7 (at 1 cM), chromosome 12 (at 13 cM), and chromosome 13 (at 45 cM). The transcript abundance QTL were not linked to their respective structural genes. Interval mapping on chromosome 7 reveals substantial overlap between QTL that control AVP and Crf-R1 transcript abundance and on chromosome 12 for QTL that control CRF and Crf-R1, which may indicate loci that coordinate regulation of the CRF system. There are QTL for all four targets on chromosome 12. There are a number of neurodevelopmental genes in very close proximity to the transcript abundance QTL that are potential candidate genes.
p66Shc Expression in Proliferating Thyroid Cells Is Regulated by Thyrotropin Receptor Signaling
Authors: Park Y. J. , Kim T. Y. , Lee S. H. , Kim H. , Kim S. W. , Shong M. , Yoon Y. K. , Cho B. Y. , Park D. J. 
Published: February 2005

AbstractIt is almost unanimously accepted that thyrocyte proliferation is synergistically activated by TSH and insulin/IGF-I. Moreover, it was recently suggested that p66Shc, which is an adaptor molecule of the IGF-I receptor, might play a critical role in this synergistic effect. In this study, we undertook to confirm the role and the mechanism underlying the regulation of p66Shc expression via TSH receptor in thyrocytes. We have found that p66Shc expression is elevated in proliferating human thyroid tissues, including adenomatous goiter, adenoma, Graves’ disease, and thyroid cancer, but not in normal thyroid. Among growth factors, TSH increased p66Shc expression both in vivo and in vitro; however, IGF-I, epidermal growth factor, or insulin did not. TSH and Graves’ Ig increased the p66Shc expression via the TSH receptor-Gs-cAMP pathway. However, interestingly, IGF-I or epidermal growth factor increased the tyrosine phosphorylations of p66Shc, and this was enhanced by TSH pretreatment. A similar synergism was observed during the DNA synthesis. When we measured the p66Shc levels induced by individual Igs from 130 patients with Graves’ disease, TSH receptor stimulating activity and goiter size showed a weak correlation. We conclude that the expression of p66Shc is regulated by signaling through the TSH receptor in proliferating thyroid cells and that p66Shc appears to be an important mediator of the synergistic effect between TSH and IGF-I with respect to thyrocyte proliferation. Moreover, we suggest that TSH potentiates the regulatory effect of IGF-I on thyrocyte growth, at least in part, by increasing the expression of p66Shc.
Development of Self-Assembling Nucleators for Highly Transparent Semi-Crystalline Polypropylene
Authors: Kobayashi Toshiaki , Hashimoto Takeji 
Published: February 2005

Abstract
In 1,3:2,4-bis-O-(p-methylbenzylidene)-d-sorbitol (PDTS)/molten i-PP systems, the self-assembling ramified fibrils of PDTS are formed at Tfg,c higher than Tc during the cooling process. They function as the nucleators for the nucleation of molten i-PP. The ramified fibrils have the following characteristics: i) hexagonal crystal with the lattice cell a = b = 30.2, c = 4.4 Å, and γ = 120°, ii) mass fractal structure with large specific surface area, and iii) random orientation. Thus the following properties are induced for the i-PP: i) excellent transparency so that the lamellae build a sheaf-like crystalline superstructure with a small apex angle, ii) iso-directional and improved mechanical properties, and iii) shortened molding time.
The Potentiometric Effect of Hydrazine on a Belousov–Zhabotinskii Oscillating Chemical Reaction: Application to the Determination of Hydrazine
Authors: Raoof Jahan Bakhsh , Ojani Reza , Kiani Abolfazl , Khosravi Masomeh , Adnani Atena 
Published: February 2005

Abstract
A method for determination of hydrazine based on the Belousov–Zhabotinskii reaction is proposed. The addition of the hydrazine to the oscillating system increases the oscillating amplitude. This increase in oscillating amplitude was used as the analytical signal. To obtain a maximum value of analytical signal in determination of the hydrazine, the effects of different concentrations of malonic acid, potassium bromide, potassium bromate, cerium(IV) and sulfuric acid were investigated and the optimum concentration of each component was used for further experiments. The analytical signal is linearly proportional to the logarithm of the hydrazine concentration over the range of 1 × 10−6 M to 1 × 10−3 M (1 M = 1 mol dm−3) under optimum conditions. The obtained LOD (2σ) was 1 × 10−6 M and the relative standard deviation (RSD) for five measurements of 1.1 × 10−4 M hydrazine was 5%. The influences of some potential sources of interference were also investigated.
In Situ Deposition of Co9S8 Nanocrystallite on Its Single-Crystal Flakes at Low Temperatures
Authors: Zhang Yuanguang , Guo Fan , Wan Songming , Zheng Weiwei , Peng Yiya 
Published: February 2005

Abstract
Co9S8 hexagonal aggregations were synthesized in hydrazine hydrate (N2H4·H2O) solvent at 150 °C for 12 h using cobalt(II) sulfate hydrate (CoSO4·7H2O) and sodium sulfite (Na2SO3) as starting materials through a hydrothermal controlled-reduction method. The as-prepared samples were characterized by X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM), and their magnetic properties were evaluated on a vibrating sample magnetometer (VSM). The results showed the samples were mainly composed of Co9S8 hexagonal aggregations with an average diameter of 2.2 μm and a thickness of 200 nm. About 3 nm Co9S8 nanocrystallites deposited on its single-crystal flakes. The products exhibited ferromagnetism; the saturation magnetization (Ms) and coercivity (Hc) values of the samples were 80 emu/g and 320 Oe, respectively. The possible formation mechanism of Co9S8 hexagonal aggregations is discussed.
The Causes of Different Acid Strengths in Water: A Multivariate Statistical Approach
Authors: Aruga Roberto 
Published: February 2005

Abstract
Multivariate thermodynamic data of proton dissociation of 84 aromatic acids in aqueous medium have been processed with factor analysis. It is demonstrated that the concept of latent factor allows quantitative and rigorous determinations of the number and of the importance of the causes of different acid strengths.
Novel Through-Space Conjugated Polymers Consisting of Alternate [2.2]Paracyclophane and Fluorene
Authors: Morisaki Yasuhiro , Chujo Yoshiki 
Published: February 2005

Abstract
Novel through-space π-conjugated polymers having [2.2]paracyclophane and fluorene units were synthesized by the Suzuki coupling reaction. The polymers possessed good solubility in common organic solvents and good thermal stability. According to the UV–vis absorption spectra, the polymer showed an extension of π-delocalization via the through-space with π–π stacking compared with two kinds of model compounds. All of the polymers exhibited purplish-blue emission with high photoluminescence (PL) efficiencies of 73–81% in solution. The HOMO and LUMO energy levels of the polymer were estimated from the cyclic voltammogram.
Synthesis and Reactions of Cyclic Silylboranes
Authors: Suginome Michinori , Noguchi Hiroyoshi , Hasui Tomoaki , Murakami Masahiro 
Published: February 2005

Abstract
Five-membered cyclic silylboranes, 2-(diisopropylamino)-1,1-diorgano-1-sila-2-boracyclopentanes, were synthesized via intramolecular reductive B–Si bond formation with 3-[chloro(diisopropylamino)boryl]-1-(chlorodiorganosilyl)propane. The cyclic silylboranes were thermally stable and could be distilled under a vacuum. The reaction of the cyclic silylboranes with alcohols resulted in a cleavage of the Si–B bond, whereas a reaction with sec-amines led to an amino group exchange on the boron atom. The insertion of alkynes into the Si–B bond of the cyclic silylborane was effectively catalyzed by palladium catalysts, leading to a regioselective formation of seven-membered cyclic alkenes in high yields.
Novel Functional Groups with Fine-Controlled Metal Assembling Function
Authors: Yamamoto Kimihisa , Higuchi Masayoshi , Kimoto Atsushi , Imaoka Takane , Masachika Kiriko 
Published: February 2005

Abstract
Half-substituted dendritic phenylazomethine dendrimers with various substituents (Half-DPA-X Gn, where n is the generation number) were synthesized in order to elucidate the electron gradients in the dendrons by complexation with metal ions. Along with the change in the UV–vis spectra, the isosbestic point shifted about 11 nm from 371 to 360 nm. The number of added equivalents of SnCl2 required to induce a shift was in agreement with the number of the imine sites present in each layer of Half-DPA-H G4. The degree of the electron gradient in the phenylazomethine monodendrons, Half-DPAs, was estimated via titration with trifluoroacetic acid. We found drastic differences in the basicity of the imine sites; the basicity of the core imines is higher than that of the more peripheral imines. The stepwise complexation behavior is thermodynamically controlled from the core to terminal imines in Half-DPA-H. Furthermore, Half-DPA G4 with various substituents possesses the same electron gradients in the dendron, so the complexation behavior with metal ions is the same.
Selective Catalytic Reduction of NO by Methane over Pd Loaded on Heteropolyacids/SiO2 at Low Temperature
Authors: Okumura Kazu , Yoshimoto Ryosuke , Suzuki Katsuki , Niwa Miki 
Published: February 2005

Abstract
Pd was loaded on heteropolyacids/SiO2, and used for the selective reduction of NO with methane in the presence of oxygen and moisture. The catalyst exhibited activity in the selective reduction of NO in the temperature range between 473 and 573 K. The temperature was significantly lower than that in the conventional catalysts, such as Pd-loaded zeolites or monolayer-type oxides. Among tested heteropolyacids, H3PW12O40 gave rise to the highest activity in the reaction. The catalytic performance was improved by the addition of water vapor. The generation of highly dispersed PdO species was observed in the Pd K-edge EXAFS spectra, whose structure was similar to those found in the conventional Pd-loaded zeolites catalyst. The reason for the evolution of NO reduction activity over Pd/H3PW12O40/SiO2 was attributed to the formation of NOH+, as measured by IR, in addition to the generation of highly dispersed PdO species at low temperature on the Brønsted acid sites of heteropolyacid.