bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increase CO
2
recycling of
Escherichia coli
containing CBB genes by enhancing solubility of multiple expressed proteins from an operon through temperature reduction
Authors: Yu Jaeyoung , Shin Woo-Ri , Kim Ji Hun , Lee Soo Youn , Cho Byung-Kwan , Kim Yang-Hoon , Min Jiho 
Published: October 2023

ABSTRACT

One of the current promising solutions to address problems of CO
2
emissions is the development of biological carbon fixation strategies. This strategy leads to higher chemical biosynthetic productivity through improved biological CO
2
fixation. In a previous study, we introduced the Calvin-Benson Bassham (CBB) genes of the photosynthetic bacterium
Cereibacter sphaeroides
into
Escherichia coli
to develop a strain capable of endogenous CO
2
recycling by heterologous expression of the CBB genes. However, the heterologous expression of recombinant proteins in
E. coli
is often hampered by inclusion bodies (IB), which are protein aggregates. Various factors contribute to IB formation, including host cell metabolism, protein synthesis, transformation machinery, and target protein properties. In this study, we investigated the influence of environmental conditions, particularly culture temperature, on IB formation and CO
2
recycling in the CBB strain. As a result, by reducing the culture temperature from 37°C to 30°C, a significant suppression of IB formation was achieved, resulting in a remarkable decrease in CO
2
release by approximately 5.76 times. In addition, interestingly, an enhancement in the accumulation of pyruvate by approximately 2.3 times was observed at the same time. These results demonstrate the simultaneous improvements in CO
2
recycling and the synthesis of organic acids achieved through temperature control. Based on the findings of this study, we believe that temperature control would be a promising approach to increasing chemical biosynthetic productivity as well as biological CO
2
fixation activity in integrated autotrophic biorefinery strategies.

IMPORTANCE

In a previous study, we successfully engineered
Escherichia coli
capable of endogenous CO
2
recycling through the heterologous expression of the Calvin-Benson Bassham genes. Establishing an efficient gene expression environment for recombinant strains is crucial, on par with the importance of metabolic engineering design. Therefore, the primary objective of this study was to further mitigate greenhouse gas emissions by investigating the effects of culture temperature on the formation of inclusion bodies (IB) and CO
2
fixation activity in the engineered bacterial strain. The findings demonstrate that lowering the culture temperature effectively suppresses IB formation, enhances CO
2
recycling, and concurrently increases the accumulation of organic acids. This temperature control approach, without adding or modifying compounds, is both convenient and efficient for enhancing CO
2
recycling. As such, additional optimization of various environmental parameters holds promise for further enhancing the performance of recombinant strains efficiently.
Kilometer-scale recumbent folding, tectonic attenuation, and rotational shear in the western Anaconda Range, southwestern Montana, USA
Authors: Neal Bryce A. , Laskowski Andrew K. , Lonn Jeffrey D. , Burrell William B. 
Published: October 2023

Abstract
The Eocene Anaconda metamorphic core complex is the most recently documented metamorphic core complex in the North American Cordillera. While much work has focused on constraining the nature and timing of core complex extension, earlier deformation preserved in its footwall is not as well understood. The Anaconda metamorphic core complex footwall contains an anomalously thin, lower- to uppermost-amphibolite-facies section of Mesoproterozoic Belt Supergroup and Paleozoic metasedimentary strata. While the tectonic nature of this thinning is generally accepted, the mechanisms behind it remain enigmatic. Previous workers have hypothesized that footwall strata were attenuated along the upper limb of the Late Cretaceous Fishtrap recumbent anticline, a kilometer-scale, NW-vergent, recumbent fold exposed throughout the west-central metamorphic core complex footwall. New geologic mapping in the west-central Anaconda Range better constrains the nature and timing of tectonic attenuation in this structurally complex area. Two generations of folds were recognized: (1) F1 recumbent isoclines associated with the Fishtrap recumbent anticline and (2) F2 W-vergent asymmetric folds associated with map-scale N-plunging folds. F1 folds, axial planar S1 transposition fabrics, and bedding-parallel faults and shear zones boudinage, transpose, and omit strata of the Belt Supergroup. We suggest that the Fishtrap recumbent anticline tectonically attenuated the Belt Supergroup through Paleozoic section of the west-central Anaconda metamorphic core complex footwall, and we propose that it is a kilometer-scale, regionally significant structure. We further propose that the fold may have developed in response to rotational shear and sinistral transpression along the Lewis and Clark Line, which was further driven by accretion of outboard terranes along the western margin of North America during Late Cretaceous time.
Association between depression and pain, functional disability, disease activity and health-related quality of life in patients with systemic lupus erythematosus: a meta-analysis
Authors: Zhao Qian , Li Xin , Chen Haoyang , Wang Lili , Wu Ning , Ma Ji , Shen Biyu 
Published: October 2023

ObjectiveThe aim of this study was to explore the effect of depression on pain, disease activity, functional disability and health-related quality of life measured by Visual Analogue Scale, Systemic Lupus Erythematosus Disease Activity Index, Health Assessment Questionnaire and Short Form-36 in patients with systemic lupus erythematosus.DesignMeta-analysis.Data sourcesThe PubMed, EMBASE, Cochrane Library and Web of Science were searched for obtaining available studies from inception to 7 March 2023.Eligibility criteria for selecting studiesStudies evaluating the impact of depression on pain, disease activity, functional disability and quality of life were included.Data extraction and synthesisTwo authors independently screened publications and extracted data according to set inclusion and exclusion criteria. Statistical analyses were conducted with RevMan V.5.3.0. Data were pooled using a fixed-effects or random-effects model according to heterogeneity.ResultsA total of 13 identified studies met the inclusion criteria, reporting on a total of 1911 patients with systemic lupus erythematosus. The Visual Analogue Scale score was significantly higher in patients with depression than those without depression (standardised mean difference (SMD)=0.84 (95% CI 0.27 to 1.42), p=0.004). The Health Assessment Questionnaire score was significantly higher in patients with depression than those without depression (SMD=1.05 (95% CI 0.14 to 1.95), p<0.05). The Systemic Lupus Erythematosus Disease Activity Index score was significantly higher in patients with depression than those without depression (SMD=0.46 (95% CI 0.27 to 0.64), p<0.00001). Scores in most Short Form-36 dimensions (physical function, role physical function, emotional role function, vitality, mental health, social function, general health, physical component scale, mental component scale) were lower in patients with depression than those without depression.ConclusionThis meta-analysis showed that depression was associated with increased in pain, functional disability and disease activity, and decline in health-related quality of life in patients with systemic lupus erythematosus. Awareness of the importance of the relationship between depression in systemic lupus erythematosus patients and pain, functional disability and the quality of life might assist rheumatology physicians and nurses in assessing and preventing these symptoms.PROSPERO registration numberCRD42021265694.
Barriers to climate change and health research in India: a qualitative study
Authors: Shrikhande Shreya , Merten Sonja , Cambaco Olga , Lee Tristan T , Lakshmanasamy Ravivarman , Röösli Martin , Dalvie Mohammad Aqiel , Utzinger Jürg , Cissé Guéladio 
Published: October 2023

ObjectivesAlmost a quarter of the global burden of disease and mortalities is attributable to environmental causes, the magnitude of which is projected to increase in the near future. However, in many low- and middle-income settings, there remains a large gap in the synthesis of evidence on climate-sensitive health outcomes. In India, now the world’s most populous country, little remains known about the impacts of climate change on various health outcomes. The objective of this study is to better understand the challenges faced in conducting climate change and health research in Puducherry, India.Design and settingIn this study, we employed key informant interviews to deepen the understanding of the perceived research barriers in Puducherry. The findings were analysed using data-driven qualitative thematic analysis to elaborate the major perceived barriers to conducting environmental health research.ParticipantsThis study was conducted among 16 public health professionals, including medical researchers, and professionals involved in environmental policies and planning in Puducherry.ResultsWe identify three key barriers faced by public health professionals as key stakeholders, namely: (1) political and institutional barriers; (2) education and awareness barriers; and (3) technical research barriers. We show there is a need, from the professionals’ perspective, to improve community and political awareness on climate change and health; strengthen technical research capacity and collaboration among researchers; and strengthen health surveillance, resource allocation and access to health data for research.ConclusionEvidence informed policies and interventions are a key element in the adaptation response for countries. In the context of the paucity of data on environmental health from India, despite recognised climate change related health vulnerabilities, these findings could contribute to the development and improvement of relevant interventions conducive to a strong research environment.
Nuclear GRP78 Promotes Metabolic Reprogramming and Therapeutic Resistance in Pancreatic Ductal Adenocarcinoma
Authors: Zhao Tiansuo , Jiang Tingting , Li Xiaojia , Chang Shaofei , Sun Qihui , Kong Fanyang , Kong Xiangyu , Wei Fang , He Jie , Hao Jihui , Xie Keping 
Published: October 2023

Abstract

Purpose:
Stromal fibrosis limits nutritional supply and disarrays metabolism in pancreatic cancer (PDA, pancreatic ductal adenocarcinoma). Understanding of the molecular basis underlying metabolic cues would improve PDA management. The current study determined the interaction between glucose-regulated proteins 78 (GRP78) and hypoxia-inducible factor 1α (HIF-1α) and its mechanistic roles underlying PDA response to oxygen and glucose restrains.

Experimental Design:
Gene expression and its association with clinicopathologic characteristics of patients with PDA and mouse models were analyzed using IHC. Protein expression and their regulation were measured by Western blot and immunoprecipitation analyses. Protein interactions were determined using gain- and loss-of-function assays and molecular methods, including chromatin immunoprecipitation, co-immunoprecipitation, and dual luciferase reporter.

Results:
There was concomitant overexpression of both GRP78 and HIF-1α in human and mouse PDA tissues and cells. Glucose deprivation increased the expression of GRP78 and HIF-1α, particularly colocalization in nucleus. Induction of HIF-1α expression by glucose deprivation in PDA cells depended on the expression of and its own interaction with GRP78. Mechanistically, increased expression of both HIF-1α and LDHA under glucose deprivation was caused by the direct binding of GRP78 and HIF-1α protein complexes to the promoters of HIF-1α and LDHA genes and transactivation of their transcriptional activity.

Conclusions:
Protein complex of GRP78 and HIF-1α directly binds to HIF-1α own promoter and LDHA promoter, enhances the transcription of both HIF-1α and LDHA, whereas glucose deprivation increases GRP78 expression and further enhances HIF-1α and LDHA transcription. Therefore, crosstalk and integration of hypoxia- and hypoglycemia-responsive signaling critically impact PDA metabolic reprogramming and therapeutic resistance.
Bifunctional Inhibitor Reveals NEK2 as a Therapeutic Target and Regulator of Oncogenic Pathways in Lymphoma
Authors: McCrury Mason , Swafford Kennith , Shuttleworth Sydnye L. , Mehdi Syed Hassan , Acharya Baku , Saha Debasmita , Naceanceno Kevin , Byrum Stephanie D. , Storey Aaron J. , Xu Ying-Zhi , Doshier Claire , Patel Vijay , Post Ginell R. , De Loose Annick , Rodriguez Analiz , Shultz Leonard D. , Zhan Fenghuang , Yoon Donghoon , Frett Brendan , Kendrick Samantha 
Published: October 2023

Abstract
Expression of the serine/threonine kinase never in mitosis gene A (NIMA)–related kinase 2 (NEK2) is essential for entry into mitosis via its role in facilitating centrosome separation. Its overactivity can lead to tumorigenesis and drug resistance through the activation of several oncogenic pathways, including AKT. Although the cancer-enabling activities of NEK2 are documented in many malignancies, including correlations with poor survival in myeloma, breast, and non–small cell lung cancer, little is known about the role of NEK2 in lymphoma. Here, in tumors from patients with diffuse large B-cell lymphoma (DLBCL), the most common, aggressive non-Hodgkin lymphoma, we found a high abundance of NEK2 mRNA and protein associated with an inferior overall survival. Using our recently developed NEK2 inhibitor, NBI-961, we discovered that DLBCL cell lines and patient-derived cells exhibit a dependency on NEK2 for their viability. This compromised cell fitness was directly attributable to efficient NEK2 inhibition and proteasomal degradation by NBI-961. In a subset of particularly sensitive DLBCL cells, NBI-961 induced G2/mitosis arrest and apoptosis. In contrast, an existing indirect NEK2 inhibitor, INH154, did not prevent NEK2 autophosphorylation, induce NEK2 proteasomal degradation, or affect cell viability. Global proteomics and phospho-proteomics revealed that NEK2 orchestrates cell-cycle and apoptotic pathways through regulation of both known and new signaling molecules. We show the loss of NEK2-sensitized DLBCL to the chemotherapy agents, doxorubicin and vincristine, and effectively suppressed tumor growth in mice. These studies establish the oncogenic activity of NEK2 in DLBCL and set the foundation for development of anti-NEK2 therapeutic strategies in this frequently refractory and relapse-prone cancer.
Risk Factors for Sudden Cardiac Arrest Among Hispanic or Latino Adults in Southern California: Ventura PRESTO and HCHS/SOL
Authors: Reinier Kyndaron , Moon Jee‐Young , Chugh Harpriya S. , Sargsyan Arayik , Nakamura Kotoka , Norby Faye L. , Uy‐Evanado Audrey , Talavera Gregory A. , Gallo Linda C. , Daviglus Martha L. , Hadduck Katy , Shepherd Daniel , Salvucci Angelo , Kaplan Robert C. , Chugh Sumeet S. 
Published: October 2023

Background
Out‐of‐hospital sudden cardiac arrest (SCA) is a leading cause of mortality, making prevention of SCA a public health priority. No studies have evaluated predictors of SCA risk among Hispanic or Latino individuals in the United States.

Methods and Results

In this case‐control study, adult SCA cases ages 18‐85 (n=1,468) were ascertained in the ongoing Ventura
Pre
diction of
S
udden Death in Mul
t
i‐Ethnic C
o
mmunities (PRESTO) study (2015‐2021) in Ventura County, California. Control subjects were selected from 3033 Hispanic or Latino participants who completed Visit 2 examinations (2014–2017) at the San Diego site of the HCHS/SOL (Hispanic Community Health Survey/Study of Latinos). We used logistic regression to evaluate the association of clinical factors with SCA. Among Hispanic or Latino SCA cases (n=295) and frequency‐matched HCHS/SOL controls (n=590) (70.2% men with mean age 63.4 and 61.2 years, respectively), the following clinical variables were associated with SCA in models adjusted for age, sex, and other clinical variables: chronic kidney disease (odds ratio [OR], 7.3 [95% CI, 3.8–14.3]), heavy drinking (OR, 4.5 [95% CI, 2.3–9.0]), stroke (OR, 3.1 [95% CI, 1.2–8.0]), atrial fibrillation (OR, 3.7 [95% CI, 1.7–7.9]), coronary artery disease (OR, 2.9 [95% CI, 1.5–5.9]), heart failure (OR, 2.5 [95% CI, 1.2–5.1]), and diabetes (OR, 1.5 [95% CI, 1.0–2.3]).

Conclusions
In this first population‐based study, to our knowledge, of SCA risk predictors among Hispanic or Latino adults, chronic kidney disease was the strongest risk factor for SCA, and established cardiovascular disease was also important. Early identification and management of chronic kidney disease may reduce SCA risk among Hispanic or Latino individuals, in addition to prevention and treatment of cardiovascular disease.
Road Endpoints and City Sizes
Authors: Barsanetti Bruno 
Published: October 2023

Abstract
I examine the long-run effects of the timing of railroad construction on city sizes. I first present a stylized model which predicts that towns that are railroad endpoints for longer become persistently larger. I then show that, in a sample of Brazilian railroad towns, time as endpoint strongly predicts town size: each additional year that a town was a railroad endpoint in the past is associated with a town population 0.107 log points larger in 2010. Additional testable implications of the model and an instrumental variable approach suggest that such association reflects a causal effect.
Poor Substitutes? Counterfactual Methods in IO and Trade Compared
Authors: Head Keith , Mayer Thierry 
Published: October 2023

Abstract
Constant elasticity of substitution (CES) demand for monopolistically competitive firm-varieties is a standard tool for models in international trade and macroeconomics. Inter-variety substitution in this model follows a simple share proportionality rule. In contrast, the standard toolkit in industrial organization (IO) estimates a demand system in which cross-elasticities depend on similarity in observable attributes. The gain in realism from the IO approach comes at the expense of requiring richer data and greater computational challenges. This paper uses the data generating process of Berry, Levinsohn and Pakes (1995), BLP, who established the modern IO method, to simulate counterfactual trade policy experiments. We use the CES model as an approximation of the more complex underlying demand system and market structure. Although the CES model omits key elements of the data generating process, the errors are offsetting, allowing it to fit BLP-based predictions closely. For aggregate outcomes, it turns out that incorporating non-unitary pass-through matters more than fixing over-simplified substitution patterns.
Smartphone Data Reveal Neighborhood-Level Racial Disparities in Police Presence
Authors: Chen M. Keith , Christensen Katherine L. , John Elicia , Owens Emily , Zhuo Yilin 
Published: October 2023

Abstract
Research on policing has focused on documented actions such as stops and arrests—less is known about patrols and presence. We map the neighborhood movement of nearly ten thousand officers across 21 of America's largest cities using anonymized smartphone data. Police spend 0.36% more time in neighborhoods for each percentage point increase in Black residents. This neighborhood-level disparity persists after controlling for density, socioeconomic, and crime-driven demand for policing, and may be lower in cities with more Black police supervisors (but not officers). Patterns of police presence statistically explain 57% of the higher arrest rate in more Black neighborhoods.
Between Imagination and Exegesis: The Masorah Figurata Illustrations of the Two Menorot in Vatican ebr. 14
Authors: Liss Hanna 
Published: October 2023

Abstract
This article will deal with two folios from Ms. Vatican ebr. 14, a Pentateuch (with haftarot and megillot) written by Elijah ben Berekhiah ha-Nakdan in 1239. Both folios display a seven-branched candelabrum (menorah). The one on folio 104r is depicted lying on its side, while the one on folio 155v is standing upright. Both lampstands consist of masoretic material in micrographic script. The article will address both the form and content of the masorah figurata illustrations and relate them to Elijah’s overall pedagogical concept of teaching the Bible with a special emphasis on Hebrew language, grammar, and masorah. In addition, it will address the relationship between biblical text and its visualization, and it will ask whether the concept of peshaṭ exegesis was a necessary tool for visualizing biblical objects.
Sewing Mothers: Re-envisioning Jewish-Christian Relations in Sholem Asch’s “On a Carnival Night”
Authors: Abeliovich Ruthie , Moss Yonatan 
Published: October 2023

Abstract
A close examination of early twentieth-century literature written in Hebrew and Yiddish reveals that many of its first representations of Christianity put a central emphasis on Mary. This fact has been overlooked in previous scholarship fixated on the role of Jesus. Our article aims to fill this lacuna by focusing on Mary’s appearance in a short, controversial story published in 1909 by Sholem Asch. Through a close reading of Mary’s encounter with the matriarch Rachel, we define Asch’s ‘maternal model,’ an alternative paradigm for Jewish-Christian relations. We argue that the marginalization of Mary from the scholarly conversation is not a slipup. Rather, it is part of a gendered scheme that prioritized Jesus over his mother and narratives of persecution and victimhood over care and obligation. We show how Asch’s artfully constructed fictional formation offers a political position founded on empathy and interconnectivity.
Power by Proxy
Authors: Frank Nicholas 
Published: October 2023

Abstract
Fifteen countries recently signed the Regional Comprehensive Economic Partnership (RCEP) and formed the world’s largest trade bloc between some of the globe’s largest and fastest-growing economies. Employing a text-as-data analysis, this article systematically compares the text of the RCEP to the previous agreements of its members to determine the sources of language in the RCEP and investigate why particular treaty text is replicated more frequently relative to others. The results indicate that language derived from the multiparty and multicontinental trade agreements of the United States, a state not involved in the RCEP negotiations, accounted for a disproportionate share of the finalized text. These findings highlight the temporal dimension of power asymmetries as well as the importance of treaty design itself in the diffusion of regulatory norms and suggest that specific trade agreements serve as reference points for subsequent agreements.
The Orange Tree and Postcritique in Valeria Luiselli’s Los ingrávidos
Authors: Pearson Iris 
Published: October 2023

Abstract
This essay proposes that Valeria Luiselli’s Los ingrávidos rethinks modes of critical reading in the twenty-first century, arguing that the writer trains her reader in a mode of reading which anticipates Rita Felski’s opposition to critique, relishing acceptance and deference over suspicion and interrogation. By focusing on Luiselli’s intervention into readerly practice in this way, the essay moves beyond stringent national and historicist frameworks which often risk obscuring that novelist’s experimental forms and avant-garde project.