bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of significant m6A regulators and immune microenvironment characterization in ischemic stroke
Authors: Zhao Lili , Song Dingli , Li Tao , li Ye , Dang Meijuan , Hao Qian , fan Hong , Lu Ziwei , Lu Jialiang , Heyingwang  , Wang Xiaoya , Jian Yating , Zhang Guilian 
Published: February 2024

AbstractThe role of m6A modification in the regulation of the immune microenvironment (IME) of ischemic stroke (IS) is barely known. Thus, we aim to investigate the impact of m6A modification on the IME of IS and its diagnostic value in IS. We comprehensively assessed the m6A modification patterns, the relationship between these modification patterns and the characteristics of the IME. The m6A modification patterns of individual IS sample were quantified by m6Ascore. The performance of m6A phenotype-related genes as potential biomarkers was evaluated by the area under the receiver operating characteristic curve. Experimental validation was also performed by qRT-PCR. Six dysregulated m6A regulators were identified and a classification model consisting of four key m6A regulators (METLL3, RBMX, RBM15B, YTDHF3) could distinguish IS and healthy control samples well. METTL3 and YTHDF3 are closely related to circulating neutrophil abundance. Two distinct m6A modification patterns were determined which differed in immunocyte abundance. We also identified six m6A phenotype-related genes (APOBEC3A, PTMA, FCGR3A, LOC440926, LOC649946, and FTH1L11), and further explored their biological function. Among them, APOBEC3A, FCGR3A, and FTH1L11 were positively associated with neutrophil abundance. APOBEC3A and FCGR3A were stable diagnostic m6A-associated genes in both the discovery and validation cohorts. This study reveals that m6A modification plays a non-negligible role in the formation of a diversified and complex IME in IS. The m6A phenotype-related genes could be diagnostic biomarkers of IS.
Perception of power quality disturbances using Fourier, Short-Time Fourier, continuous and discrete wavelet transforms
Authors: Priyadarshini M. S. , Bajaj Mohit , Prokop Lukas , Berhanu Milkias 
Published: February 2024

AbstractElectric power utilities must ensure a consistent and undisturbed supply of power, with the voltage levels adhering to specified ranges. Any deviation from these supply specifications can lead to malfunctions in equipment. Monitoring the quality of supplied power is crucial to minimize the impact of fluctuations in voltage. Variations in voltage or current from their ideal values are referred to as "power quality (PQ) disturbances," highlighting the need for vigilant monitoring and management. Signal processing methods are widely used for power system applications which include understanding of voltage disturbance signals and used for retrieval of signal information from the signals Different signal processing methods are used for extracting information about a signal. The method of Fourier analysis involves application of Fourier transform giving frequency information. The method of Short-Time Fourier analysis involves application of Short-Time Fourier transform (STFT) giving time–frequency information. The method of continuous wavelet analysis involves application of Continuous Wavelet transform (CWT) giving signal information in terms of scale and time where frequency is inversely related to scale. The method of discrete wavelet analysis involves application of Discrete Wavelet transform (DWT) giving signal information in terms of approximations and details where approximations and details are low and high frequency representation of original signal. In this paper, an attempt is made to perceive power quality disturbances in MATLAB using Fourier, Short-Time Fourier, Continuous Wavelet and Discrete Wavelet Transforms. Proper understanding of the signals can be possible by transforming the signals into different domains. An emphasis on application of signal processing techniques can be laid for power quality studies. The paper compares the results of each transform using MATLAB-based visualizations. The discussion covers the advantages and disadvantages of each technique, providing valuable insights into the interpretation of power quality disturbances. As the paper delves into the complexities of each method, it takes the reader on a journey of signal processing complexities, culminating in a nuanced understanding of power quality disturbances and their representations across various domains. The outcomes of this research, elucidated through energy values, 3D plots, and comparative analyses, contribute to a comprehensive understanding of power quality disturbances. The findings not only traverse theoretical domains but also find practical utility in real-world scenarios.
Sex- and age-dependent susceptibility to ventricular arrhythmias in the rat heart ex vivo
Authors: Oknińska Marta , Duda Monika Katarzyna , Czarnowska Elżbieta , Bierła Joanna , Paterek Aleksandra , Mączewski Michał , Mackiewicz Urszula 
Published: February 2024

AbstractThe incidence of life-threatening ventricular arrhythmias, the most common cause of sudden cardiac death (SCD), depends largely on the arrhythmic substrate that develops in the myocardium during the aging process. There is a large deficit of comparative studies on the development of this substrate in both sexes, with a particular paucity of studies in females. To identify the substrates of arrhythmia, fibrosis, cardiomyocyte hypertrophy, mitochondrial density, oxidative stress, antioxidant defense and intracellular Ca2+ signaling in isolated cardiomyocytes were measured in the hearts of 3- and 24-month-old female and male rats. Arrhythmia susceptibility was assessed in ex vivo perfused hearts after exposure to isoproterenol (ISO) and hydrogen peroxide (H2O2). The number of ventricular premature beats (PVBs), ventricular tachycardia (VT) and ventricular fibrillation (VF) episodes, as well as intrinsic heart rate, QRS and QT duration, were measured in ECG signals recorded from the surfaces of the beating hearts. After ISO administration, VT/VFs were formed only in the hearts of males, mainly older ones. In contrast, H2O2 led to VT/VF formation in the hearts of rats of both sexes but much more frequently in older males. We identified several components of the arrhythmia substrate that develop in the myocardium during the aging process, including high spontaneous ryanodine receptor activity in cardiomyocytes, fibrosis of varying severity in different layers of the myocardium (nonheterogenic fibrosis), and high levels of oxidative stress as measured by nitrated tyrosine levels. All of these elements appeared at a much greater intensity in male individuals during the aging process. On the other hand, in aging females, antioxidant defense at the level of H2O2 detoxification, measured as glutathione peroxidase expression, was weaker than that in males of the same age. We showed that sex has a significant effect on the development of an arrhythmic substrate during aging. This substrate determines the incidence of life-threatening ventricular arrhythmias in the presence of additional stimuli with proarrhythmic potential, such as catecholamine stimulation or oxidative stress, which are constant elements in the pathomechanism of most cardiovascular diseases.
Identification of new pharmacophore against SARS-CoV-2 spike protein by multi-fold computational and biochemical techniques
Authors: Ullah Atta , Ullah Saeed , Halim Sobia Ahsan , Waqas Muhammad , Ali Basharat , Ataya Farid S. , El-Sabbagh Nasser M. , Batiha Gaber El-Saber , Avula Satya Kumar , Csuk Rene , Khan Ajmal , Al-Harrasi Ahmed 
Published: February 2024

AbstractCOVID-19 appeared as a highly contagious disease after its outbreak in December 2019 by the virus, named SARS-CoV-2. The threat, which originated in Wuhan, China, swiftly became an international emergency. Among different genomic products, spike protein of virus plays a crucial role in the initiation of the infection by binding to the human lung cells, therefore, SARS-CoV-2’s spike protein is a promising therapeutic target. Using a combination of a structure-based virtual screening and biochemical assay, this study seeks possible therapeutic candidates that specifically target the viral spike protein. A database of ~ 850 naturally derived compounds was screened against SARS-CoV-2 spike protein to find natural inhibitors. Using virtual screening and inhibitory experiments, we identifiedacetyl 11-keto-boswellic acid(AKBA) as a promising molecule for spike protein, which encouraged us to scan the rest of AKBA derivatives in our in-house database via 2D-similarity searching. Later 19 compounds with > 85% similarity with AKBA were selected and docked with receptor binding domain (RBD) of spike protein. Those hits declared significant interactions at the RBD interface, best possess and excellent drug-likeness and pharmacokinetics properties with high gastrointestinal absorption (GIA) without toxicity and allergenicity. Ourin-silicoobservations were eventually validated by in vitro bioassay, interestingly, 10 compounds(A3, A4, C3, C6A, C6B, C6C, C6E, C6H, C6I,andC6J)displayed significant inhibitory ability with good percent inhibition (range: > 72–90). The compoundsC3(90.00%),C6E(91.00%),C6C(87.20%), andC6D(86.23%) demonstrated excellent anti-SARS CoV-2 spike protein activities. The docking interaction of high percent inhibition of inhibitor compoundsC3andC6Ewas confirmed by MD Simulation. In the molecular dynamics simulation, we observed the stable dynamics of spike protein inhibitor complexes and the influence of inhibitor binding on the protein’s conformational arrangements. The binding free energy ΔGTOTALofC3(−38.0 ± 0.08 kcal/mol) andC6E(−41.98 ± 0.08 kcal/mol) respectively indicate a strong binding affinity to Spike protein active pocket. These findings demonstrate that these molecules particularly inhibit the function of spike protein and, therefore have the potential to be evaluated as drug candidates against SARS-CoV-2.
Majority of human circulating IgG plasmablasts stop blasting in a cell-free pro-survival culture
Authors: Nguyen Doan C. , Saney Celia , Hentenaar Ian T. , Cabrera-Mora Monica , Capric Violeta , Woodruff Matthew C. , Andrews Joel , Lonial Sagar , Sanz Ignacio , Lee F. Eun-Hyung 
Published: February 2024

AbstractFollowing infection or vaccination, early-minted antibody secreting cells (ASC) or plasmablasts appear in circulation transiently, and a small fraction migrates to the spleen or bone marrow (BM) to mature into long-lived plasma cells (LLPC). While LLPC, by definition, are quiescent or non-dividing, the majority of blood ASC are thought to be “blasting” or proliferative. In this study, we find > 95% nascent blood ASC in culture express Ki-67 but only 6–12% incorporate BrdU after 4 h or 24 h labeling. In contrast, < 5% BM LLPC in culture are Ki-67+ with no BrdU uptake. Due to limitations of traditional flow cytometry, we utilized a novel optofluidic technology to evaluate cell division with simultaneous functional IgG secretion. We find 11% early-minted blood ASC undergo division, and none of the terminally differentiated BM LLPC (CD19−CD38hiCD138+) divide during the 7–21 days in culture. While BM LLPC undergo complete cell cycle arrest, the process of differentiation into an ASC or plasmablasts also discourages entry into S phase. Since the majority of Ki-67+ nascent blood ASC have exited cell cycle and are no longer actively “blasting”, the term “plasmablast”, which traditionally refers to an ASC that still has the capacity to divide, may probably be a misnomer.
Associations between fears related to safety during sleep and self-reported sleep in men and women living in a low-socioeconomic status setting
Authors: Correia Arron T. L. , Forshaw Philippa E. , Roden Laura C. , Lipinska Gosia , Rauch H. G. Laurie , Lambert Estelle V. , Layden Brian T. , Reutrakul Sirimon , Crowley Stephanie J. , Luke Amy , Dugas Lara R. , Rae Dale E. 
Published: February 2024

AbstractSouth Africans living in low socioeconomic areas have self-reported unusually long sleep durations (approximately 9–10 h). One hypothesis is that these long durations may be a compensatory response to poor sleep quality as a result of stressful environments. This study aimed to investigate whether fear of not being safe during sleep is associated with markers of sleep quality or duration in men and women. South Africans (n = 411, 25–50 y, 57% women) of African-origin living in an urban township, characterised by high crime and poverty rates, participated in this study. Participants are part of a larger longitudinal cohort study: Modelling the Epidemiologic Transition Study (METS)–Microbiome. Customised questions were used to assess the presence or absence of fears related to feeling safe during sleep, and the Epworth Sleepiness Scale, Pittsburgh Sleep Quality Index (PSQI) and Insomnia Severity Index were used to assess daytime sleepiness, sleep quality and insomnia symptom severity respectively. Adjusted logistic regression models indicated that participants who reported fears related to safety during sleep were more likely to report poor sleep quality (PSQI > 5) compared to participants not reporting such fears and that this relationship was stronger among men than women. This is one of the first studies outside American or European populations to suggest that poor quality sleep is associated with fear of personal safety in low-SES South African adults.
Comparative assessment of direct and indirect cold atmospheric plasma effects, based on helium and argon, on human glioblastoma: an in vitro and in vivo study
Authors: Bakhtiyari-Ramezani Mahdiyeh , Nohekhan Mojtaba , Akbari Mohammad Esmaeil , Abbasvandi Fereshteh , Bayat Mahdis , Akbari Atieh , Nasiri Meysam 
Published: February 2024

AbstractRecent research has highlighted the promising potential of cold atmospheric plasma (CAP) in cancer therapy. However, variations in study outcomes are attributed to differences in CAP devices and plasma parameters, which lead to diverse compositions of plasma products, including electrons, charged particles, reactive species, UV light, and heat. This study aimed to evaluate and compare the optimal exposure time, duration, and direction-dependent cellular effects of two CAPs, based on argon and helium gases, on glioblastoma U-87 MG cancer cells and an animal model of GBM. Two plasma jets were used as low-temperature plasma sources in which helium or argon gas was ionized by high voltage (4.5 kV) and frequency (20 kHz). In vitro assessments on human GBM and normal astrocyte cell lines, using MTT assays, flow cytometry analysis, wound healing assays, and immunocytochemistry for Caspase3 and P53 proteins, demonstrated that all studied plasma jets, especially indirect argon CAP, selectively induced apoptosis, hindered tumor cell growth, and inhibited migration. These effects occurred concurrently with increased intracellular levels of reactive oxygen species and decreased total antioxidant capacity in the cells. In vivo results further supported these findings, indicating that single indirect argon and direct helium CAP therapy, equal to high dose Temozolomide treatment, induced tumor cell death in a rat model of GBM. This was concurrent with a reduction in tumor size observed through PET-CT scan imaging and a significant increase in the survival rate. Additionally, there was a decrease in GFAP protein levels, a significant GBM tumor marker, and an increase in P53 protein expression based on immunohistochemical analyses. Furthermore, Ledge beam test analysis revealed general motor function improvement after indirect argon CAP therapy, similar to Temozolomide treatment. Taken together, these results suggest that CAP therapy, using indirect argon and direct helium jets, holds great promise for clinical applications in GBM treatment.
ARID2 mutations may relay a distinct subset of cutaneous melanoma patients with different outcomes
Authors: Akinjiyan Favour A. , Nassief George , Phillipps Jordan , Adeyelu Tolulope , Elliott Andrew , Abdulla Farah , Zhou Alice Y. , Souroullas George , Kim Kevin B. , Vanderwalde Ari , Park Soo J. , Ansstas George 
Published: February 2024

AbstractARID genes encode subunits of SWI/SNF chromatin remodeling complexes and are frequently mutated in human cancers. We investigated the correlation between ARID mutations, molecular features, and clinical outcomes in melanoma patients. Cutaneous melanoma samples (n = 1577) were analyzed by next-generation sequencing. Samples were stratified by pathogenic/likely pathogenic mutation in ARID genes (ARID1A/2/1B/5B). PD-L1 expression was assessed using IHC (SP142; positive (+): ≥ 1%). Tumor mutation burden (TMB)-high was defined as ≥ 10 mutations/Mb. Transcriptomic signatures predictive of response to immune checkpoint inhibitors—interferon gamma and T-cell inflamed score were calculated. Real-world overall survival (OS) information was obtained from insurance claims data, with Kaplan–Meier estimates calculated from time of tissue collection until last date of contact. Mann–Whitney U, Chi-square, and Fisher exact tests were applied where appropriate, with p values adjusted for multiple comparisons. ARID2 mutations were more prevalent in cutaneous melanoma compared to ARID1A (11.0%: n = 451 vs 2.8%: n = 113), with concurrent ARID1A/ARID2 mutation in 1.1% (n = 46) of samples. ARID mutations were associated with a high prevalence of RAS pathway mutations—NF1 (ARID1A, 52.6%; ARID2, 48.5%; ARID1A/2, 63.6%; and ARID-WT, 13.3%; p < 0.0001) and KRAS (ARID1A, 3.5%; ARID2, 3.1%; ARID1A/2, 6.5%; and ARID-WT, 1.0%; p = 0.018)), although BRAF mutations were less common in ARID-mutated cohorts (ARID1A, 31.9%; ARID2, 35.6%; ARID1A/2, 26.1%; and ARID-WT, 50.4%; p < 0.0001). TMB-high was more common in ARID-mutated samples (ARID1A, 80.9%; ARID2, 89.9%; ARID1A/2, 100%; and ARID-WT, 49.4%; p < 0.0001), while PD-L1 positivity was similar across subgroups (ARID1A, 43.8%; ARID2, 51.1%; ARID1A/2, 52.5%; and ARID-WT, 44.9%; p = 0.109). Patients with ARID1A mutations had a higher prevalence of dMMR/MSI-H compared to those with ARID-WT (2.7% vs 0.2%, p = 0.030). Median IFN-γ and T-cell signatures were higher in ARID2-mutated samples compared to ARID-WT (IFN-γ: − 0.15 vs − 0.21, p = 0.0066; T-cell: 23.5 vs − 18.5, p = 0.041). ARID2-mutated patients had improved survival compared to ARID-WT; (HR: 1.22 (95% CI 1.0–1.5), p = 0.022). No additional OS benefit was observed with anti-PD-1 therapy for ARID2 mutation compared to ARID-WT. Melanoma patients with ARID mutations exhibited higher prevalence of markers associated with ICI response, including TMB-H, and immune-related signatures. Our data also suggests improved survival outcome in patients with ARID2 mutations, irrespective of anti-PD1 therapy.
Research on variable universe fuzzy PID control for semi-active suspension with CDC dampers based on dynamic adjustment functions
Authors: Ji Guanggang , Zhang Lidong , Cai Mingda , Meng Xianke , Du Zhengyu , Ruan Jiuhong , Guan Shenhao , Liu Zhiwen 
Published: February 2024

AbstractThe vehicle suspension system is a complex system with multiple variables, nonlinearity and time-varying characteristics, and the traditional variable universe fuzzy PID control algorithm has the problems of over-reliance on expert experience and non-adaptive adjustment of the contracting-expanding factor parameters, which make it difficult to achieve a better control effect. In this paper, the system error e(t) and its change rate ec(t) are introduced into the contracting-expanding factor as dynamic parameters to realize the adaptive adjustment of the contracting-expanding factor parameters, and propose a variable universe fuzzy PID control based on dynamic adjustment functions (VUFP-DAF), which uses the real-time contracting-expanding factor to realize the adaptive adjustment of the fuzzy universe, so as to improve the ride comfort of vehicles. The research results show that the proposed VUFP-DAF has strong adaptability and can effectively improve the ride comfort and handling stability of vehicles under different speeds and road excitations, providing a certain technical basis for the development of the semi-active suspension system.
Isotopic analysis of formula milk reveals potential challenges in geolocating bottle-fed babies
Authors: Kootker Lisette M. , Ammer Saskia T. M. , Davies Gareth R. , Lehn Christine 
Published: February 2024

AbstractIn forensic investigations involving the identification of unknown deceased individuals, isotope analysis can provide valuable provenance information. This is especially pertinent when primary identifiers (i.e., DNA, dactyloscopy, etc.) fail to yield matches. The isotopic composition of human tissues is linked to that of the food consumed, potentially allowing the identification of regions of origin. However, the isotopic composition of deceased newborns and infants fed with milk formula may be influenced by that of the prepared milk. The findings contribute towards the possibility to isotopically identify bottle-fed infants. More importantly, the data convincingly show that the Sr isotope composition of the prepared milk is determined by that of the formula and not the (local) tap water, thereby limiting the potential of Sr isotope analysis for determining the geological or geographical origin in formula-fed babies in medico-legal cases.
Subjectively salient faces differ from emotional faces: ERP evidence
Authors: Żochowska Anna , Nowicka Anna 
Published: February 2024

AbstractThe self-face is processed differently than emotional faces. A question arises whether other highly familiar and subjectively significant non-self faces (e.g. partner’s face) are also differentiated from emotional faces. The aim of this event-related potential (ERP) study was to investigate the neural correlates of personally-relevant faces (the self and a close-other’s) as well as emotionally positive (happy) and neutral faces. Participants were tasked with the simple detection of faces. Amplitudes of N170 were more negative in the right than in the left hemisphere and were not modulated by type of face. A similar pattern of N2 and P3 results for the self-face and close-other’s face was observed: they were associated with decreased N2 and increased P3 relative to happy and neutral faces. However, the self-face was preferentially processed also when compared to a close-other’s face as revealed by lower N2 and higher P3 amplitudes. Nonparametric cluster-based permutation tests showed an analogous pattern of results: significant clusters for the self-face compared with all other faces (close-other’s, happy, neutral) and for close-other’s face compared to happy and neutral faces. In summary, the self-face prioritization was observed, as indicated by significant differences between one’s own face and all other faces. Crucially, both types of personally-relevant faces differed from happy faces. These findings point to the pivotal role of subjective evaluation of the saliency factor.