bluuebunny/crossref_metadata_2025_split huggingface dataset:
Defensibility and ethics in the laboratory
Authors: Boyd Jo Ann 
Published: May 2003

AbstractLaboratory personnel must feel that ethical standards of conduct are a priority in the laboratory. Management must support this concept not only during training but also in daily communication to employees. Further, review of staff experience and training of employees is necessary to reinforce the need for ethics. This paper discusses defensibility and ethics in the laboratory, and the goals of assuring that the reporting of laboratory data will be performed in an ethical manner and will provide legal defensibility of data and laboratory procedures. Copyright © 2003 John Wiley & Sons, Ltd.
What's New?
Authors: Bohaychuk Wendy , Broughton Steve 
Published: May 2003

AbstractThe What's New? section in the Quality Assurance Journal aims to draw attention to items of importance to QA professionals. There is a three‐month interval between final submission of manuscripts and distribution of the journal. For this issue, the entries in the What's New? Section arise from the period of 01 December 2002 to 28 February 2003. If you feel that there is any other new information that should be brought to the attention of readers, please let us know by e‐mailing wbohaychuk@gcrpc.com Copyright © 2003 John Wiley & Sons, Ltd.
The effect of wood and temperature on juvenile coho salmon winter movement, growth, density and survival in side‐channels
Authors: Giannico Guillermo R. , Hinch Scott G. 
Published: May 2003

AbstractIn British Columbia, side‐channels have been built to compensate for lost salmonid habitat. Most are structurally simple with little in‐stream wood; however, they support high densities of juvenile coho salmon. We longitudinally divided in halves the top 100 m of two dead‐end artificial side‐channels, one side‐channel with low winter water temperatures (surface‐fed) and one with relatively higher water temperatures (groundwater‐fed), closed the downstream end of each side‐channel with two‐way traps, and treated only one half of each channel with bundles of wood. Trapped fish were marked daily and coho salmon movement, growth and smolt output were monitored for two years. Wood addition increased juvenile coho winter carrying capacity and spring smolt output only in the ‘colder’ surface‐fed side‐channel. In contrast, in the groundwater‐fed side‐channel, with relatively higher water temperatures, the wood treatment slightly reduced the channel's carrying capacity and the spring output of coho salmon smolts. Copyright © 2003 John Wiley & Sons, Ltd.
Rehabilitating the floodplain of the lower River Dyje for fish
Authors: Lusk Stanislav , Halačka Karel , Lusková Věra 
Published: May 2003

AbstractThe lower reaches of the River Dyje were heavily modified between 1975 and 1985. The river was straightened, isolating its meanders and reducing its length by 22%. As a consequence, the river bed showed a markedly lower diversity in both hydrological and morphological characteristics. Because the River Dyje forms the state boundary between the Czech Republic and Austria, fluvial activity of the lower reaches is excluded. Along the River Dyje an active alluvium has been preserved, with approximately 1300 hectares in the Czech Republic and somewhat less in Austria. The flow regime in the lower reaches is regulated by 19 water reservoirs and a number of fishponds, with a total capacity of approximately 45% of the annual drainage. These modifications have distinctly impacted the living conditions of the indigenous ichthyofauna. Based on the results of our ichthyological investigations from 1994 to 2000, we have recommended a number of modifications that could improve the environmental conditions for fishes: (i) controlled flooding of the alluvial meadows, aimed at creating conditions for the spawning of phytophilous fish species; (ii) restoration of permanent connections between separated river meanders and the River Dyje to increase the hydrological and morphological diversity in the river bed; (iii) creation of artificial pools in the floodplain to provide more suitable habitats for fishes. Copyright © 2003 John Wiley & Sons, Ltd.
Desnagging to resnagging: new directions in river rehabilitation in southeastern Australia
Authors: Erskine Wayne D. , Webb Ashley A. 
Published: May 2003

AbstractExtensive desnagging (removal of large woody debris and living riparian vegetation) and associated river improvement works were conducted in rivers of southeastern Australia (Victoria and New South Wales) between at least 1886 and 1995. Swamp drainage, large woody debris removal and vegetation clearing were strongly supported by legislation, government funding and institutional arrangements in both states. As a result, large amounts of large woody debris were removed from rivers, regenerating indigenous vegetation was cleared from within designed alignment widths and, ironically, huge numbers of exotic trees, especially willows, were planted. The environmental impacts of desnagging have only been documented on a few impacted rivers but have included increased flow velocity, spatially extensive bed degradation, massive channel enlargement and loss of fish habitat. Recognition of the need for more integrated land and water management, and new research on the hydraulic, geomorphic, biogeographic and ecological significance of large woody debris and the values of indigenous riparian vegetation during the 1980s led to a major shift in river rehabilitation. We have drawn on our own and other published research to further develop a set of guidelines for the incorporation of large woody debris into river rehabilitation plans. Our guidelines extend those recently prepared for southeastern Australia and address site selection, where to place timber, the amount to be introduced, how to distribute it, techniques of introduction and woody debris sources. However, in the long term, riparian vegetation rehabilitation within the potential recruitment zone is essential to supply large woody debris. Given that our results demonstrate that very large woody debris makes a significant contribution to the total loading, it will be a very long time (>100 years) before natural recruitment can be recreated. Copyright © 2003 John Wiley & Sons, Ltd.
A 180,000-year pollen record from Owens Lake, CA: terrestrial vegetation change on orbital scales
Authors: Woolfenden Wallace B. 
Published: May 2003

AbstractPollen from the upper 90 m of core OL-92 from Owens Lake is a climatically sensitive record of vegetation change that indicates shifts in the plant associations representing warm and cold desertscrub, pinyon–juniper woodland, and pine–fir forest during the past 180,000 years. These changes are synchronized with glacial–interglacial cycles. During glacial and stadial climates, juniper woodland expanded downslope and replaced warm desert shrubs while upper montane and subalpine forests in the arid Inyo Mountains also expanded, and those in the Sierra Nevada were displaced by the ice cap and periglacial conditions. Conversely, during interglacial and interstadial climates, warm desert plants expanded their range in the lowlands, juniper and sagebrush retreated upslope, and montane and subalpine forests expanded in the Sierra Nevada. The reconstructed vegetation history demonstrates a regional climatic response, and the congruence of the pollen sequence with marine and ice cap oxygen isotope stratigraphies suggests a link between regional vegetation and global climate change at orbital scales.
Holocene climate and landscape evolution East of the Pechora Delta, East-European Russian Arctic
Authors: Väliranta Minna , Kaakinen Anu , Kuhry Peter 
Published: May 2003

AbstractThis study presents a multiproxy record of Holocene environmental change in the region East of the Pechora Delta. A peat plateau profile (Ortino II) is analyzed for plant macrofossils, sediment type, loss on ignition, and radiocarbon dating. A paleosol profile (Ortino III) is described and radiocarbon dated. A previously published peat plateau profile (Ortino I) was analyzed for pollen and conifer stomata, loss on ignition, and radiocarbon dating. The interpretation of the latter site is reassessed in view of new evidence. Spruce immigrated to the study area at about 8900 14C yr B.P. Peatland development started at approximately the same time. During the Early Holocene Hypsithermal taiga forests occupied most of the present East-European tundra and peatlands were permafrost free. Cooling started after 5000 14C yr B.P., resulting in a retreat of forests and permafrost aggradation. Remaining forests disappeared from the study area around 3000 14C yr B.P., coinciding with more permafrost aggradation. The retreat of forests resulted in landscape instability and the redistribution of sand by eolian activity. The displacement of the Arctic forest line and permafrost zones indicates a warming of at least 2–3°C in mean July and annual temperatures during the Early Holocene. At least two cooling periods can be recognized for the second half of the Holocene, starting at about 4800 and 3000 14C yr B.P.
Phytolith analysis from the archaeological site of Kush, Ras al-Khaimah, United Arab Emirates
Authors: Ishida Sachiko , Parker Adrian G. , Kennet Derek , Hodson Martin J. 
Published: May 2003

AbstractDespite the wealth of archaeological sites and excellent conditions for preservation, few phytolith investigations have been undertaken from the Arabian Gulf region. The results from the Sasanian and Islamic archaeological tell of Kush, Ras al-Khaimah, United Arab Emirates, are presented. Kush is situated just inside the Gulf on an important trade route. The occupation sequence dates from the 4th century A.D. until the 13th century A.D., recording the development of the site in the Sasanian period, followed by the arrival of Islam in the 7th century A.D. and the final abandonment of the site in the late 13th century when the nearby site of al-Mataf (Julfar) began to develop closer to the present day coastline. All the samples analyzed contained abundant phytoliths (short cells, elongated cells, and groups of elongated cells) of various types. They included date palm (Phoenix dactylifera), papillae (possibly from barley (Hordeum)), and hair cells possibly from species of canary grass (Phalaris spp.). Some researchers have suggested that groups of elongated cells may indicate the presence of irrigation in semiarid environments. The present results for this class of phytoliths appeared to imply that intensive irrigation was unlikely to have taken place around Kush.
Influence of age, castration, and testosterone on T cell subsets in healthy and leukemia grafted mice
Authors: Aboudkhil Souad , Zaîd Abdelhamid , Henry Laurent , Bureau Jean‐Paul 
Published: May 2003

Abstract The distribution of T cell subsets in pubertal (2 months) and post‐pubertal (10 months) mice showed a significant decrease in the percentage of CD4+ splenocytes and peripheral blood lymphocytes (PBL) with age, unlike the percentage of CD8+ cells in PBL, which remained unchanged. The change in the distribution of T cell subsets in the spleen and blood occurred in 2 months old castrated mice, as in 10 months old animals. P388 tumor grew better in post‐pubertal and in castrated mice than in young mice. The intact mice survived longer than the castrated ones. The relative number of CD4+, CD8+ and CD2+ splenocytes was lower in transplanted intact mice than that in controls. The CD8+ and CD2+ subsets in the blood of 2 months transplanted mice were higher than those in controls, whereas in PBL, in 10 months old and castrated mice, the T lymphocyte subsets remain unchanged. Depo‐testosterone (DT) injection strongly reduced weight and tumor growth in all the intact and castrated animals. A significant correlation is observed between the tumor weight and testosterone level in the plasma of the 2 months old DT treated mice. Moreover, DT injection induced a significant increase in the percentage of blood CD8+ cells in all the batches. These data indicate that physiologically, androgens affect the age‐related distribution of lymphocyte T subsets and suggest that they slow down tumor growth, besides causing a direct effect, through an immunological process.
Cardiorespiratory system dynamics in chronic heart failure
Authors: Mangin L. , Monti A. , Médigue C. 
Published: May 2003

AbstractAims:With the complex demodulation (CDM) method, we assessed the instantaneous amplitude and frequency of cardiovascular (CV) and respiratory oscillations, and the instant phase (IP) between the CV and respiratory signals using respiration as a periodic forced stimulation. We hypothesised a possible lack of synchronisation between CV and respiratory signals under regular breathing at different frequencies.Methods:RR interval (ECG), blood pressure (SBP/DBP, Finapress), respiration (Respitrace) were monitored during two random‐order periods of voluntary paced‐breathing (0.15 Hz/0.25 Hz) in 10 moderate CHF patients and 10 age‐matched controls. The CDM method provides the amplitude and frequency of a particular spectral component as a function of time in both LF and HF bands. IP between CV and respiratory oscillations was assessed using the real modulating breathing rate.Results:(i) Continuous phase variations between CV oscillations and the respiratory signal were evidenced in CHF patients, the slower the breathing rate, the greater the phase variation (RR/Resp; 0.25 Hz, 23±17°; 0.15 Hz, 46±57°, P<0.01; RR/Resp at 0.15 Hz 6±3 vs. 46±57 P<0.01 controls vs. CHF). Phase was constant in controls. (ii) In patients, the instant amplitude of the cardiovascular oscillations in the high frequency domain is more markedly altered when the breathing rate was slowed down as compared to controls.Conclusion:The lack of synchronisation between physiological signals during voluntary breathing in CHF patients highlights a central uncoupling between CV and respiratory neuronal activities.
Factors of home dream recall: a structural equation model
Authors: Schredl Michael , Wittmann Lutz , Ciric Petra , GÖtz Simon 
Published: May 2003

SummaryPrevious research has indicated that personality factors such as openness to experience, creativity, visual memory, attitude toward dreams, and sleep behavior is related to home dream recall frequency (DRF). However, a study investigating all areas simultaneously within one sample in order to determine the percentage of variance explained by all variables and to take intercorrelations between the influencing factors into account has not been performed till now. The present study with 444 participants fills this gap. Using several indicators for each of the variables mentioned above, a structural equation model was tested. Although the model fit was satisfying, the four factors which were significantly related to DRF: personality (openness to experience, thin boundaries, absorption), creativity, nocturnal awakenings, and attitude toward dreams, explained only 8.4% of the total variance. As this value is considerably lower than those of studies investigating a single influencing factor and using similar measurement instruments in similar samples, one might speculate about possible expectancy effects in these previous studies, an effect which has been demonstrated for DRF in the laboratory setting. In addition, the small percentage of explained variance of each single factors (<3%) may indicate that other, in this study unmeasured, variables such as sleep duration (state aspect), introspection, and cognitive functioning immediately upon awakening (sleep inertia) show substantial covariance with the interindividual differences in DRF. Future studies should focus on longitudinal aspects in order to differentiate between state versus trait factors (although methodologic issues, e.g. the effect of the measurement technique on DRF itself, have to be clarified) and investigate additional variables which might be associated with DRF (see above).
Image size magnification and power and dilation factors for optical instruments in general
Authors: Harris W. F. 
Published: May 2003

AbstractTraditional treatments of spectacle magnification for distant objects consider only stigmatic spectacle lenses and they compare the retinal image size in a refractively fully compensated eye with the image size in the uncompensated eye. Spectacle magnification is expressed as a product of two factors, the power and shape factors of the lens. The power factor depends on the position of the entrance pupil of the eye. For an eye with an astigmatic cornea, however, the position of the entrance pupil is not well defined. Thus, the traditional approach to spectacle magnification does not generalize properly to allow for astigmatism. Within the constraints of linear optics and subject to the restriction that the eye's iris remains the aperture stop, this paper provides a complete, unified and exact treatment for optical instruments in general. It compares retinal image size in a generalized sense (including image shape and orientation) for any instrument in front of an eye with that of the eye alone irrespective of whether the instrument compensates or not. The approach does not make use of the concept of the entrance pupil at all and it allows for astigmatism and for non‐alignment of refracting elements in the instrument and in the eye. The concept of spectacle magnification generalizes to the concept of instrument size magnification. Instrument size magnification can be expressed as the product of two matrix factors one of which can be interpreted as a power factor (as back‐vertex power) and the other factor for which the name dilation factor is more appropriate in general. The general treatment is then applied to a number of special cases including afocal instruments, spectacle lenses (including obliquely crossing thick bitoric lenses), contact lenses, stigmatic systems and stigmatic eyes. In the case of spectacle lenses, the dilation factor reduces to the usual shape factor.
Haplotype Structure, LD Blocks, and Uneven Recombination Within the LRP5 Gene
Authors: Twells Rebecca C.J. , Mein Charles A. , Phillips Michael S. , Hess J. Fred , Veijola Riitta , Gilbey Matthew , Bright Matthew , Metzker Michael , Lie Benedicte A. , Kingsnorth Amanda , Gregory Edward , Nakagawa Yusuke , Snook Hywel , Wang William Y.S. , Masters Jennifer , Johnson Gillian , Eaves Iain , Howson Joanna M.M. , Clayton David , Cordell Heather J. , Nutland Sarah , Rance Helen , Carr Philippa , Todd John A. 
Published: May 2003

Patterns of linkage disequilibrium (LD) in the human genome are beginning to be characterized, with a paucity of haplotype diversity in “LD blocks,” interspersed by apparent “hot spots” of recombination. Previously, we cloned and physically characterized thelow-density lipoprotein-receptor-related protein 5(LRP5) gene. Here, we have extensively analysed bothLRP5 and its flanking three genes, spanning 269 kb, for single nucleotide polymorphisms (SNPs), and we present a comprehensive SNP map comprising 95 polymorphisms. Analysis revealed high levels of recombination across LRP5, including a hot-spot region from intron 1 to intron 7 of LRP5, where there are 109 recombinants/Mb (4882 meioses), in contrast to flanking regions of 14.6 recombinants/Mb. This region of high recombination could be delineated into three to four hot spots, one within a 601-bp interval. ForLRP5, three haplotype blocks were identified, flanked by the hot spots. Each LD block comprised over 80% common haplotypes, concurring with a previous study of 14 genes that showed that common haplotypes account for at least 80% of all haplotypes. The identification of hot spots in between these LD blocks provides additional evidence that LD blocks are separated by areas of higher recombination.[Supplementary material: primers are available from our Web site:http://www-gene.cimr.cam.ac.uk/todd/human_data.shtml.]
Comparative Analysis of Vertebrate Dystrophin Loci Indicate Intron Gigantism as a Common Feature
Authors: Pozzoli Uberto , Elgar Greg , Cagliani Rachele , Riva Laura , Comi Giacomo P. , Bresolin Nereo , Bardoni Alessandra , Sironi Manuela 
Published: May 2003

The human DMD gene is the largest known to date, spanning > 2000 kb on the X chromosome. The gene size is mainly accounted for by huge intronic regions. We sequenced 190 kb of Fugu rubripes(pufferfish) genomic DNA corresponding to the complete dystrophin gene (FrDMD) and provide the first report of gene structure and sequence comparison among dystrophin genomic sequences from different vertebrate organisms. Almost all intron positions and phases are conserved between FrDMD and its mammalian counterparts, and the predicted protein product of the Fugu gene displays 55% identity and 71% similarity to human dystrophin. In analogy to the human gene, FrDMD presents several-fold longer than average intronic regions. Analysis of intron sequences of the human and murine genes revealed that they are extremely conserved in size and that a similar fraction of total intron length is represented by repetitive elements; moreover, our data indicate that intron expansion through repeat accumulation in the two orthologs is the result of independent insertional events. The hypothesis that intron length might be functionally relevant to the DMD gene regulation is proposed and substantiated by the finding that dystrophin intron gigantism is common to the three vertebrate genes.[Supplemental material is available online at www.genome.org.]
Discovering Novel cis-Regulatory Motifs Using Functional Networks
Authors: Ettwiller Laurence M. , Rung Johan , Birney Ewan 
Published: May 2003

We combined functional information such as protein–protein interactions or metabolic networks with genome information inSaccaromyces cerevisiae to predict cis-regulatory motifs in the upstream region of genes. We developed a new scoring metric combining these two information sources and used this metric in motif discovery. To estimate the statistical significance of this metric, we used brute-force randomization, which shows a consistent well-behaved trend. In contrast, real data showed complex nonrandom behavior. With conservative parameters we were able to find 42 degenerate motifs (that touch 40% of yeast genes) based on 647 original patterns, five of which are well known. Some of these motifs also show limited spatial position in the promoter, indicative of a true motif. We also tested the metric on other known motifs and show that this metric is a good discriminator of real motifs. As well as a pragmatic motif discovery method, with many applications beyond this work, these results also show that interacting proteins are often coordinated at the level of transcription, even in the absence of obvious coregulation in gene expression data sets.[Supplemental material is available online athttp://www.ebi.ac.uk/∼ettwille/genome_research_paper_2003/result_overlap.html. Program available upon request.]