bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of CMTM6 on the anti-HCC efficacy of iNKT cells via stabilizing PD-L1 in an experimental investigation
Authors: Teng Jingfang , Wang Yaqi , Zhang Min , Li Zheng , Liu Qiyao , Lv Wenping , Meng Ming 
Published: October 2023

Abstract
Background CMTM6 is a key regulator of PD-L1. The purpose of this study is to see whether CMTM6 impacts iNKT cells infiltration into hepatocellular carcinoma (HCC) and whether CMTM6 knockout promotes iNKT cells activation via the PD-1/PD-L1 signaling axis, hence impacting anti-HCC efficacy.
Methods Immunohistochemistry and multiplex fluorescence immunohistochemistry were conducted to evaluate the expression of CMTM6, PD-1, PD-L1, and iNKT (CD3+CD56+) cells. Flow cytometry (FCM), enzyme-linked immunosorbent assay (ELISA), cell proliferation, killing, colony formation assay, and wound healing assay were applied to investigate the effect of CMTM6 knockout on the anti-HCC efficacy of iNKT cells.
Results In HCC tumor tissues, there's far more CMTM6, PD-L1, and PD-1 expression. PD-L1 and PD-1 were both positively associated with CMTM6. iNKT cells infiltration was reduced, whereas PD-1 expression was increased. Infiltration of iNKT cells was reduced by increased CMTM6 expression. CMTM6 knockout inhibited the proliferation, invasion, and migration of HepG2 cells by downregulating the PD-1/PD-L1 signaling axis and promoting the secretion of IFN-γ, Perforin, and Granzyme B of iNKT cells, boosting their killing effect, and inhibiting the proliferation, invasion, and migration of HepG2 cells.
Conclusions Our findings showed that CMTM6 was more abundant in HCC tumor tissues than adjacent tissues. Knocking out CMTM6 could boost iNKT cells activation and improve anti-HCC efficacy by blocking the PD-1/PD-L1 signaling axis, which may serve as a potential therapeutic target for HCC
Impact of Affordable Care Act provisions on the racial makeup of patients enrolled at a Deep South, high-risk breast cancer clinic
Authors: Tinglin Jillian , McLeod M. Chandler , Williams Courtney P. , Tipre Meghan , Rocque Gabrielle , Crouse Andrew B. , Krontiras Helen , Gutnik Lily 
Published: October 2023

Abstract
Purpose
Black women are less likely to receive screening mammograms and are at a higher lifetime risk for developing breast cancer compared to their White counterparts. Affordable Care Act (ACA) provisions decreased cost sharing for women’s preventive screening, potentially mitigating screening disparities. We examined enrollment of a high-risk screening program before and after ACA implementation stratified by race.
Methods
This retrospective, quasi-experimental study examined the ACA’s impact on patient demographics at a high-risk breast cancer screening clinic from 02/28/2003–02/28/2019. Patient demographic data were abstracted from electronic medical records and descriptively compared in the pre- and post-ACA time periods. Interrupted time series (ITS) analysis using Poisson regression assessed yearly clinic enrollment rates by race using incidence rate ratios (IRR) and 95% confidence intervals (CI).
Results
2,767 patients enrolled in the clinic. On average, patients were 46 years old (SD, ± 12), 82% were commercially insured, and 8% lived in a highly disadvantaged neighborhood. In ITS models accounting for trends over time, Prior to ACA implementation, White patient enrollment was stable (IRR 1.01, 95% CI 1.00-1.02) while Black patient enrollment increased at 13% per year (IRR 1.13, 95% CI 1.05–1.22). Compared to the pre-ACA enrollment period, the post-ACA enrollment rate remained unchanged for White patients (IRR 0.99, 95% CI 0.97–1.01) but decreased by 17% for Black patients (IRR 0.83, 95% CI 0.74–0.92).
Conclusion
Black patient enrollment decreased at a high-risk breast cancer screening clinic post-ACA compared to the pre-ACA period, indicating a need to identify factors contributing to racial disparities in clinic enrollment.
Correlation-based network analysis combined with machine learning techniques highlights rosmarinic acid biosynthesis activation in tuber of potato plants corresponding to water recovery treatment
Authors: Maruenda Helena , Toubiana David 
Published: October 2023

Abstract

Water-deficit, as a consequence of elongated drought periods due to current climate trends, is one of the most imminent abiotic stresses limiting crop productivity worldwide. Also, the production of potato – the world’s fourth most important crop – is challenged by the effects of water-deficit. To investigate metabolic pathway activity in the potato tuber subjected to water-deficit, a correlation-based network analysis combined with machine-learning techniques approach was conducted on a subset of samples of a potato association panel, grown in an augmented block design under normal irrigation and water-deficit conditions in Ica, Peru. Metabolic profiles for all samples of the subset were generated using a cold injection GC-TOF-MS platform identifying unequivocally 685 metabolites. Samples were separated into positive spectrum (PS) and negative spectrum (NS) groups based on their projection on principal component 2 (PC2) using principal component analysis. Correlation networks showed a strong discrepancy of edge number between the PS and NS correlation network (143,478 vs. 185,565 edges). Correlation-based network analysis combined with machine learning techniques highlighted the activity of metabolic pathways centered around tyrosine and α-ketoglutarate suggestive for an increased activity of the rosmarinic acid biosynthesis I and II pathways in the potato tuber. Our study suggests that the potato tuber copes with drought stress by rewiring its metabolic network and boosting rosmarinic acid biosynthesis to counteract ROS activity and by that ensuring the onset of the next life cycle.
Robotic Assisted Orbital Surgery for resection of advanced periocular tumours– a case series report on the feasibility, safety and outcome
Authors: Malik Mohsan , Daniel Claire , FAULKNER JACK , Uddin Jimmy , Arora Asit , Jeannon Jean Pierre 
Published: October 2023

Abstract
Purpose Orbital surgery benefits from well-designed instrumentation that offers gentle tissue manipulation, high manoeuvrability and control. Nevertheless, in confined spaces, tissue manipulation must be accomplished with exceptionally high accuracy and precision. This is where robotic surgery offers an advantage. We aimed to evaluate a robotic-assisted surgical system's feasibility, safety and outcome in assisting tumour clearance.Patients and methods A case series of patients with advanced periocular tumours undergoing robotic-assisted globe-sparing resection was performed using the DaVinci XI system (intuitive surgical, inc). Institutional ethics and multidisciplinary approval were sought in all cases.Results Four patients with advanced periocular tumours underwent robotic-assisted orbital surgery at a mean age of 63 years (range 42–86). Two patients were diagnosed with squamous cell carcinoma, and two had basal cell carcinoma. One patient was found to have positive lymph nodes at the time of surgery and underwent simultaneous parotidectomy and lymph node clearance. Clear resection of the primary tumour was achieved in all patients; three patients underwent further resection due to narrow margins prior to reconstruction. Patients were follow-up for at least one year, and three remained disease-free. One patient with pre-existing extra-orbital disease developed metastatic disease four months post-op. All patients preserved vision peri-operatively, with no complaints of diplopia. Moderate ocular surface disease was noted in two patients.Conclusion Our series highlights the potential advantage of three-dimensional optics, multi-directional instrumentation and motion scaling technology to achieve globe-sparing tumour resection in advanced periocular tumours. However, further robotic instrumentation development is required for orbital surgery.
Association Between Physical Activity in Its Different Domains and Eating Habits in Female Breast Cancer Survivors
Authors: Caldeira Daniela T. , Vanderlei Luiz Carlos M. , Palma Mariana R. , Ribeiro Fernanda E. , Tebar William R. , Mota Jorge , Ferrari Gerson , Tosello Giuliano T. , Christofaro Diego G. D. 
Published: October 2023

Abstract
Healthy eating patterns associated with physical activity (PA) are important factors for the survival of breast cancer survivors. However, little is known about whether the different domains of PA (occupational, leisure/sport and commuting) can interfere with healthy eating. For this, 128 breast cancer survivors were evaluated. The socioeconomic class was analyzed using the questionnaire of the Brazilian Association of Research Companies (ABEP). The eating habits and the frequency of weekly food intake were assessed through the Block questionnaire, while the level of PA through the Baecke questionnaire and the time spent on sedentary behavior through the participants' reports. It was found that breast cancer survivors with higher levels of leisure / sport PA presented higher consumption of fruits (β = 0.32; CI = 0.02; 0.62), cereals (β = 0.79; CI = 0.47; 1.10) and white meat (β = 0.31; CI = 0.05; 0.58), similar results were observed with displacement PA. Higher levels of PA were related to higher consumption of vegetables (β = 0.28; CI = 0.05; 0.50) and lower consumption of candies (β = -0.33; CI = -0.59; -0.08). It is concluded that breast cancer survivors who practice more PA in their different domains had a higher consumption of healthy foods.
Chemical composition and biological activities Assessment (antioxidant, antimicrobial, anti-inflammatory and healing wounds activities) of crude extract of roots of Paeonia mascula subsp. atlantica (Coss.) Greuter &amp; Burdet = (Paeonia algeriensis Chabert)
Authors: wafa Nouioua , sofiane Gaamoune , ouarda Djaout , nouioua wafa 
Published: October 2023

AbstractBackground:Paeonia algeriensisChabert is an Algerian endemic species habitually used by local people in traditional medicine. This study tried to express phytochemicals composition and biological activities of the species. CG/Ms was used to determine molecular composition, antioxidant activity was assessed by three tests (DPPH, reducing power and lipid peroxidation), antimicrobial activity was tested with four bacterial strains and one yeast, the anti-inflammatory activity was carried out by the human red blood cell (HRBC) membrane stabilization method and theIn-vivohealing wounds activity by excision model.Results:The result show a perfect antioxidant activity, a powerful antibacterial effect an important anti-inflammatory power at a low concentration and total healing wounds effect in just 13 days of experiment.Conclusions:The above results indicate that the roots ofPaeonia masculahave great potential in the development of new drugs.
Correlation between 8-OHdG and disease-free survival in patients with esophageal squamous cell carcinoma following neoadjuvant chemotherapy
Authors: Kunimitsu Atsushi , Fujishima Fumiyoshi , Sasano Hironobu , Mozumi Takeru , Higashimoto Iku , Akaishi Ryujiro , Koseki¹ Ken , Ishida Hitoraka , Ozawa¹ Yohei , Sato Chiaki , Okamoto¹ Hiroshi , Taniyama¹ Yusuke , Kamei¹ Takashi , Suzuki² Takashi 
Published: October 2023

Abstract
Background: Esophageal squamous cell carcinoma (ESCC) is an aggressive malignancy. Neoadjuvant chemotherapy (NAC) followed by surgery is the standard treatment strategy for the patients with stage II or III ESCC, but many develop resistance to treatment. Recent studies have suggested a correlation between therapeutic resistance and enhancement of the nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway in cancer cells but its details, including the antioxidant pathways which regulate the oxidative stress response after NAC, are unknown. Therefore, in this study, we investigated the status of heme oxygenase-1 (HO-1), a target of the Nrf2 pathway, and 8-hydroxy-2-deoxyguanosine (8-OHdG), an oxidative stress marker, in patients with ESCC who underwent NAC, and explored treatment strategies with relation to the findings and clinical outcomes and its correlation to clinical or histologic responsiveness.
Methods: HO-1 and 8-OHdG were immunolocalized in 99 patients with ESCC who received NAC with cisplatin and 5-fluorouracil (CF) prior to esophagectomy. Among them, 53 had lymph node metastasis at the time of surgery.
Results: Kaplan­­–Meier curves revealed that overall survival (OS) and disease-free survival (DFS) were both significantly prolonged in patients with ESCC and low HO-1 primary tumor H-score compared to those with high scores. DFS was significantly prolonged in the group with low 8-OHdG levels compared to that with high scores. Multivariate analysis demonstrated that high 8-OHdG levels adversely affected DFS in an independent fashion. In addition, Univariate analysis showed significantly prolonged OS and DFS in ESCC patients with higher histological effects of NAC, but multivariate analysis revealed that only regional lymph node metastasis (pN) was significantly correlated with OS and DFS.
Conclusions: HO-1 and 8-OHdG levels in the primary tumor were significantly correlated with NAC response in patients with ESCC. In addition, a relatively high 8-OHdG level in tumors after NAC could predict poor DFS.
Trial registration: Accession No. 2022-1-779. The trial was retrospectively registered.
Measuring the equilibrium relationships among urbanization, output growth, and environmental quality in Nigeria
Authors: Posu Sunday Mauton Ayodamola , Adeniyi Ademola Israel , Olubiyi Ebenezer Adesoji , Ojo Olutunji Timothy , Obisanya Adesile Adesegun 
Published: October 2023

Abstract
This study investigates the long-run relationship and short-run dynamics among urbanization, output growth, and environmental quality in Nigeria. The study adopts autoregressive distributed lag, bounds test co-integration analysis and the autoregressive integrated moving average with the annual time series dataset for 1990-2021. A long-run equilibrium relationship among urbanization, output growth, and environmental quality is established. Urban population, output growth, per capita GDP, and population growth are all significantly related to CO2 emissions. The study confirms the Environmental Kuznets Curve (EKC) hypothesis on per capita GDP and CO2 emissions in Nigeria. The positive interactive effect of urban population and output growth reduces CO2 emissions just as an increase in per capita GDP in a previous period produces a short-run effect of reducing CO2 emissions. The first-period lagged change in per capita GDP reports a marginal statistically significant negative effect on current CO2 emissions. The study utilizes data on past CO2 emissions to predict how CO2 emissions might evolve in the coming years and to examine the environmental sustainability of Nigeria. The forecast of CO2 emissions for 2023-2030 are relatively stable with some marginal fluctuations, which confirm the validated EKC. To sustain the validated inverted U-shaped EKC and to avoid it assuming an N-shape in the future, this study recommends environmental policy focus to monitor the growth of large cities and to implement measures capable of mitigating CO2 emissions by decoupling economic growth from CO2 emissions; and by encouraging investment in clean energy sources, sustainable transportation, and industrial practices.
JEL ClassificationE23 . O44 . O33 . Q28 . Q56 . R11
Phenotyping wild potatoes for photosynthesis associated traits under heat stress
Authors: Bashir Ikram , Nicolao Rodrigo , Haerter Janni André , de Brito Giovani Greigh , Castro Caroline , Heiden Gustavo 
Published: October 2023

Abstract
Heat stress is one of the major factors reducing potato yield by limiting the plant photosynthesis activity, production and partitioning of assimilates to the sink. Potato wild relatives (Solanum sect. Petota, Solanaceae) are a natural reservoir of valuable traits for abiotic stress resistance. However, it is necessary to assess the response of wild potato genotypes based on physiological features such as gas exchange, chlorophyll index and chlorophyll fluorescence that are promising candidates to improve crop photosynthesis under adverse conditions. Multivariate approaches on two factor experimental design were used to phenotype the best performing genotypes for most important traits under control and heat stress treatments. From the results, the most important traits to be focused on are net photosynthetic rates, transpiration rates, and stomatal conductance along with effective photochemical yield of PSII, Coefficient of photochemical yield of PSII and dry matter content, which are highly associated with heat stress conditions. Solanum commersonii accession (BGB451) and S. chacoense (BGB467, BGB113, BGB444, BGB083) are the top five genotypes of the heat stress tolerant group. However, BRSBEL, BGB003, BGB091 and BGB101 are most susceptible to heat stress. From our study, we concluded that most genotypes phenotyped for heat stress resistance belong to S. chacoense. The assessment of wild accessions for photosynthetic traits under heat stress is a reliable way to discriminate the most promising sources of abiotic stress resistance even though they do not have best quality tuber traits for the breeding of the potato crop.
Vacuum Sealing Drainage against surgical site infection after intracranial neurosurgery: a technical note
Authors: Ren Sen , Luo Yun , Shen Xiaoyong , Wu Qian , Wu Xiaohui , Ma Chao , Xiong Zhongwei , Gong Rui , Liu Zheng , chen jincao , Wang Wei 
Published: October 2023

Abstract
Background: Surgical site infections (SSIs)are still a challenge to neurosurgeons. However, Vacuum Sealing Drainage (VSD), which has been used extensively in the treatment of various infections after surgery, looks a promising solution. This study examines the efficacy and outcome of VSD treatment of pyogenic SSIs following intracranial neurosurgery.
Methods: 20 patients with infections who underwent surgical intervention were treated retrospectively using VSD at the Zhongnan hospital of Wuhan University over the past five years. Primary surgery types, SSI types, VSD replacements, surgical procedures, pathogenic germs, antibiotic therapy and infection control were reviewed and discussed.
Results: Of the 20 infections, 13 (65%) were extradural, and 7 (35%) were extradural SSIs combined with intracranial infections (including 5 meningitis, 1 subdural abscess, and 1 brain abscess). All the patients consented to medical device implantation (including 5 titanium webs, 6 bone flap fixation devices and 12 dura-plasties), with most of the devices removed during debridement. The median duration from primary surgery to a SSI diagnosis was 19 days (range: 7 to 365 days). All the patients also agreed to debridement and VSD treatment; VSD was replaced 0 to 5 times (median, one time) every 4 to 7 days and retained for 4 to 35 days (median, 14 days). Seven (35%) patients had defined bacterial infections, with staphylococcus aureus the dominant infection. The deployed standard VSD and antibiotic treatment ensured full recovery from SSIs, including from intracranial infections: 14 (70%) patients were free of infection during follow-up, and no infection-associated death was registered; 6 (30%) patients died of severe primary affections.
Conclusion: VSD-assisted therapy is safe and effective against SSIs after intracranial neurosurgery.
The Effect of Floating Chamber Design on Co2 Flux Measurements
Authors: Lee Daryl Jia Jun , ZHAN Li Yang , WONG Wei Wen , POH Seng-Chee 
Published: October 2023

Abstract
The floating chamber is valued for its simplicity, affordability, and adaptability for widespread use in measuring diffusive greenhouse gases flux from surface water, although its effectiveness may vary based on its chamber design. This study investigates the effect of chamber insulation on gas flux accumulation. Our findings reveal that non-insulated chambers experienced greater solar heating effects in their headspace, with a 20% deviation from the targetted water temperature. In contrast, insulated chambers showed less than 1% temperature difference with ambient condition. Solar-induced heating of the chamber walls increased the internal temperature, leading to an overestimation of greenhouse gas fluxes. The developed floating chambers were tested at a hydropower reservoir in Terengganu, Malaysia. Results from two distinct seasonal sampling campaigns at three sites demonstrate that CO2 fluxes during the wet season (45 mmol m-2d-1) were almost three times higher compared to the dry season (16 mmol m-2d-1).
The Clinical survival benefit of postmastectomy radiotherapy patients screened from T1-2N1M0 breast cancer according to tumor size and the number of lymph nodes
Authors: Wu Nisha , Li Junyan , Zhang Hongying , Su Xiaohan , Tan Qiao , Hou Lingmi , Chen Maoshan 
Published: October 2023

Abstract
Purpose
our study was aimed at systematically evaluated the advantage of the treatment of postmastectomy radiotherapy (PMRT) on the overall survival (OS) depending on the lymph nodes number and the size of tumors in patients who had breast cancer in stage of T1-2N1.
Results
There were 29,366 patients included in the last analysis, involving 20,167 (68.7%) cases in the group without PMRT and 9199 (31.3%) cases in the group with PMRT. Multivariable Cox model analysis presented that the treatment of radiotherapy could improve the OS (Hazard ratio HR, 0.89;95%CI 0.83–0.96, P = 0.001)in patients who had breast cancer in stage of T1-2N1M0. The analysis of subgroups according to different categories of tumor burden showed that PMRT did not provide an overall survival advantage in tumor size and lymph node subgroups. The advantages of PMRT are related with an increase in overall survival rate in patients who had tumors 4–5 cm in size and 2 or 3 positive lymph nodes (HR 0.69; 95%CI 0.51–0.94; P = 0.017or HR 0.65; 95%CI 0.44–0.95; P = 0.027, respectively). Patients who had tumors size 2–3 cm and 3 positive lymph nodes benefited from PMRT as well (HR 0.61; 95%CI 0.48–0.78; P < 0.001), but other subgroups of patients did not benefit from PMRT .
Conclusion
The size of breast cancer tumors and positive nodes number appear to be receivable forecast of overall survival benefits after the treatment of radiotherapy, which may allow clinicians to carry out more accurate and effective PMRT decisions in the clinic, especially for the subgroup who had the low-risk breast cancer.
Methods
Using data from the SEER program from 2004 to 2015, we calculated the use of PMRT and survival in patients with breast cancer who were in stage of T1-T2 and had 1–3 positive lymph nodes that were diagnosed with invasive breast cancer and underwent mastectomy. Patients were selected according to whether they received postoperative radiotherapy. And 29366 matched patients enrolled in the final analysis were respectively included in the post-mastectomy radiotherapy group (PMRT) and non-radiotherapy group (no-PMRT). We analyzed patients in subgroup analysis who had different positive nodes number and the size of tumors. Overall survival (OS) was calculated with the Kaplan-Meier method in comparison with the survival between different subgroups. We carried out Multivariate Cox proportional hazard models to evaluate the analysis of factors on prognosis and estimated the hazard ratios (HR) and their 95% confidence intervals (CI).