bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wild-type bone marrow cells repopulate tissue resident macrophages and reverse the impacts of homozygous CSF1R mutation
Authors: Carter-Cusack Dylan , Huang Stephen , Keshvari Sahar , Patkar Omkar , Sehgal Anuj , Allavena Rachel , Byrne Robert A. J. , Morgan B. Paul , Bush Stephen J. , Summers Kim M. , Irvine Katharine M. , Hume David A. 
Published: January 2025

Adaptation to existence outside the womb is a key event in the life of a mammal. The absence of macrophages in rats with a homozygous mutation in the colony-stimulating factor 1 receptor (Csf1r) gene (Csf1rko) severely compromises pre-weaning somatic growth and maturation of organ function. Transfer of wild-type bone marrow cells (BMT) at weaning rescues tissue macrophage populations permitting normal development and long-term survival. To dissect the phenotype and function of macrophages in postnatal development, we generated transcriptomic profiles of all major organs of wild-type and Csf1rko rats at weaning and in selected organs following rescue by BMT. The transcriptomic profiles revealed subtle effects of macrophage deficiency on development of all major organs. Network analysis revealed a common signature of CSF1R-dependent resident tissue macrophages that includes the components of complement C1Q (C1qa/b/c genes). Circulating C1Q was almost undetectable in Csf1rko rats and rapidly restored to normal levels following BMT. Tissue-specific macrophage signatures were also identified, notably including sinus macrophage populations in the lymph nodes. Their loss in Csf1rko rats was confirmed by immunohistochemical localisation of CD209B (SIGNR1). By 6-12 weeks, Csf1rko rats succumb to emphysema-like pathology associated with the selective loss of interstitial macrophages and granulocytosis. This pathology was reversed by BMT. Along with physiological rescue, BMT precisely regenerated the abundance and expression profiles of resident macrophages. The exception was the brain, where BM-derived microglia-like cells had a distinct expression profile compared to resident microglia. In addition, the transferred BM failed to restore blood monocyte or CSF1R-positive bone marrow progenitors. These studies provide a model for the pathology and treatment of CSF1R mutations in humans and the innate immune deficiency associated with prematurity.
The Japanese encephalitis virus NS1′ protein facilitates virus infection in mosquitoes
Authors: Liu Yuwei , Huang Yutian , Li Ruidong , Miao Chang , He Yi , Xu Changhao , Zhu Xi , Li Bowen , Wu Rui , Zhao Qin , Wen Yiping , Huang Xiaobo , Yan Qi-gui , Lang Yi-fei , Zhao Shan , Wang Yiping , Hu Yajie , Cao San-jie , Du Senyan 
Published: January 2025

Background
The Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is known for its capacity to cause severe neurological disease in Asia. Neurotropic flaviviruses within the Japanese encephalitis (JE) serogroup possess the distinctive feature of expressing a unique nonstructural protein, NS1′. The NS1′ protein consists of the full NS1 protein with an additional 52 amino acid extension at the C-terminus and has been demonstrated to exhibit virulence in mammalian hosts upon infection. However, the precise role of the NS1′ protein in the mosquito vectors has yet to be elucidated.

Methodology/principal findings
In this study, an NS1′-defective virus (rG66A) was engineered, and its effect on the infection of mosquito cells was investigated. The results demonstrated a significant reduction in the infectivity of the rG66A virus in mosquito cells by RT-qPCR, indicating that the absence of the NS1′ protein impedes JEV replication in Culex mosquitoes. Additionally, this research elucidated the underlying mechanism by which the NS1′ protein enhances viral infection in mosquitoes by RNA-Seq analysis. Specifically, the NS1′ protein was found to facilitate infection through the suppression of antimicrobial peptides (AMPs) regulated by the Toll pathway.

Conclusions/significance
Our research demonstrated that the JEV NS1′ protein contributes to immune escape, thereby enhancing viral infection in mosquitoes. This finding offers new insights into the transmission mechanisms of JEV, elucidating novel aspects of viral propagation.
Factors associated with receiving a Functional Disorder diagnostic label: A systematic review
Authors: Tattan Mais , Rosmalen Judith , Hanssen Denise 
Published: January 2025

Objectives
Functional Disorders (FD) are highly prevalent conditions that are diagnosed based on the presence of specific patterns of somatic symptoms. Examples of FDs include Fibromyalgia and Irritable Bowel Syndrome. Many patients who meet the criteria do not receive a formal diagnostic label. This systematic review aims to assess factors associated with receiving an FD diagnostic label.

Methods
A systematic search of PubMed, PsycINFO, and Embase was performed following the PRISMA guidelines. All research methodologies and languages were included with a focus on experiences and impacts of receiving/having an FD diagnostic label. Excluded studies were those not mentioning diagnostic labels, only involving single pain symptoms, and studies solely focusing on functional neurological symptoms. Screening, data extraction and quality ratings (using the QuADS instrument) were performed by two independent reviewers.

Results
15 Studies were identified (10 quantitative and 5 qualitative). Our results show that female patients were more likely to receive an FD diagnostic label for their symptoms; other associations were less consistent and only found for specific labels or research designs. In general, quality of life and healthcare use did not seem to differ between patients with and without an FD diagnostic label. From the healthcare professional’s perspective there was doubt about giving an FD diagnostic label, mainly due to concerns of harm for patients. Quality of included studies was rated low to moderate.

Conclusion
Better understanding of factors associated with receiving or having an FD diagnostic label, independently from symptom development can help healthcare professionals make evidence-based decisions in labelling or not; however, high quality studies on this topic are urgently needed.
Optimal aspiration pressure of suction pump for oocyte retrieval in infertile patients undergoing in vitro fertilization
Authors: Kim Sung Woo , Kim Hye Yun , Han Ji Yeon , Kim Hoon , Ku Seung-Yup 
Published: January 2025

Background
The oocyte retrieval is a critical step in assisted reproductive technologies, including in vitro fertilization and fertility preservation. Despite evolving techniques, the optimal aspiration pressure during retrieval remains debatable, with limited in vivo human studies. Existing studies, primarily in vitro and on animals, suggest that inappropriate aspiration pressures can impair oocyte quality. This study aims to compares the effects of two different aspiration pressures, 120mmHg and 150mmHg, on oocyte recovery, damage, and subsequent embryo development and pregnancy outcomes in infertile women undergoing transvaginal ultrasound-guided oocyte retrieval.

Methods and findings
This retrospective study analyzed data from 891 women at Seoul National University Hospital between May 2018 and August 2023. A total of 400 cycles were included, with 202 at 120 mmHg and 198 at 150 mmHg aspiration pressures. The primary outcomes were the number of retrieved, matured, fractured oocytes, embryos, and good-grade embryos. Pregnancy outcomes were evaluated by comparing the clinical pregnancy rates and live birth rates. Univariate and multivariate logistic regression analyses were conducted to evaluate the relationship between aspiration pressure, clinical pregnancy, and live birth rates. There was statistically significant difference in the number of retrieved oocytes and mature oocytes between the 120 mmHg group and the 150 mmHg group (6.3±5.2 vs. 7.7±6.7, p = 0.018; 4.4±3.7 vs. 5.6±5.3, p = 0.011). The number of embryos and good grade embryos also differed significantly (3.3±3.1 vs. 4.2±3.9, p = 0.011; 1.0±1.6 vs. 1.5±2.6, p = 0.031). However, there were no significant differences in clinical pregnancy and live birth rates between the two groups in multivariate logistic regression analysis (adjusted OR = 0.725, p = 0.519; adjusted OR = 0.370, p = 0.170).

Conclusions
Increasing the aspiration pressure to 150mmHg led to a higher yield of oocytes and embryos than 120mmHg, without any negative impact on oocyte quality or live birth rates. These findings provide valuable insights for clinical decision-making in infertility treatments, suggesting that 150mmHg may be a more effective pressure for oocyte retrieval in in vitro fertilization and embryo transfer.
Synbiotics effects of d-tagatose and Lactobacillus rhamnosus GG on the inflammation and oxidative stress reaction of Gallus gallus based on the genus of cecal bacteria and their metabolites
Authors: Lv Yuanqiang , Chu Jie , Zhang Xiaoxiao , Li Xuan , Yin Aijiao ,   on behalf of The Industrial Microbiology Laboratory
Published: January 2025

Backgrounds
Abuse of feed supplement can cause oxidative stress and inflammatory responses in Gallus gallus. Synbiotics are composed of prebiotics and probiotics and it possess huge application potentials in the treatment of animal diseases.

Methods
This study examined the effect of d-tagatose on the probiotic properties of L. rhamnosus GG, L. paracasei, and S. lactis so as to screen the best synbiotic combinations. Treat Gallus gallus exhibiting oxidative stress and immune response caused by aflatoxin b1 with optimal synbiotics for 14 days, detect the changes of inflammatory markers and oxidative stress markers of Gallus gallus using qRT-PCR, and identified the intestinal bacteria genera and their metabolites in the cecum of Gallus gallus using gut microbiota and metabolomics analysis.

Results and conclusion
The results indicated that oxidative stress and immune response factor expressions quantity in Gallus gallus decreased significantly after 14 days of treatment, compared with model group, the low-dose treatment group’s SOD1, SOD3, GPX1, GPX2, GSR, H6DP, and HO-1 genes in liver were downregulated by 36.03%, 40.01%, 45.86%, 40.79%, 37.68%, 25.04%, and 29.89%, the IL-1, IL-2, IL-4, IL-6, IgA, IgM, and IgG genes in blood and spleen were downregulated by 26.59%, 34.19%, 21.19%, 28.18%, 35.93%, 12.67%, 21.81 and 35.93%, 22.85%, 21.19%, 28.78%, 35.93%, 15.36%, 29.73%. The intestinal bacteria genera and metabolomics analysis results indicated that the abundance of beneficial bacteria genus was up-regulated, and the proportion of pathogenic bacteria genera decreased. The amount of beneficial metabolites associated with antioxidant and anti-inflammatory effects was upregulated. The synbiotic composed of d-tagatose and L. rhamnosus GG can treat oxidative stress and immune response by altering the structure of intestinal bacteria genera and the production of metabolites.
Patterns of self-harm presentations at a Tertiary Urban Hospital in Kenya: A retrospective 5-year study (2018–2022)
Authors: Njoroge Willie , Gitau Catherine , Onchari Everline , Ongeri Linnet , Khakali Linda , Khan Murad , Merali Zul , Atwoli Lukoye , Shah Jasmit 
Published: January 2025

Introduction
Self-harm represents a complex and multifaceted public health issue of global significance, exerting profound effects on individuals and communities alike. It involves intentional self-poisoning or self-injury with or without the motivation to die. Although self-harm is highly prevalent, limited research has focused on the patterns and trends of self-harm among hospital populations in low- and middle-income countries, particularly within Africa. This study aims to explore the socio-demographic and clinical profile of patients presenting with self-harm and determine the common self-harm patterns at a tertiary facility in Kenya.

Methodology
We carried out a descriptive retrospective study and included patients from inpatient units and outpatient settings within the Secion of Psychiatry at the Aga Khan University Hospital, Nairobi from January 1st 2018 to December 31st 2022. A data abstraction tool was used to collect data from eligible files sourced from the medical records department for all patients who met the study criteria. Summary statistics were reported as frequencies and percentages for categorical data and as means and standard deviations for continuous data.

Results
A total of 507 files were reviewed in the given timeframe and 497 patients were included in the analysis. Of these patients, 28.1% (n = 144) presented with self-harm. The mean age of the self-harm patients was 26.5 years (SD = 10.5) and a majority (74.3%) were female. The first point-of-contact was at the emergency department in 72.9% of the cases. A majority of them, i.e. 89.6%, reported a past psychiatric diagnosis. Based on the psychiatric diagnosis evaluation of the patients- depression was the most common diagnosis at 88.2%, followed by anxiety disorder at 27.8% and bipolar mood disorder at 17.4%. The majority of reported self-harm cases involved overdose incidents (68.8%), with self-injury accounting for 56.3% of cases. Analgesics were the most frequently reported type of overdose, followed by tricyclic antidepressants. In context of self-injury, cutting emerged as the predominant form of self-harm. Family conflict was reported to be the most common reason for self-harm at 39.6%.

Conclusion
This study shows a high rate of self-harm among patients with mental illness in this facility, necessitating the development of self-harm prevention and management protocols. A national registry of self-harm behavior would also help further elucidate the occurrence and mechanisms of self-harm in the population, improving the possibility for early interventions and prevention.
The impact of depression and anxiety disorders on postoperative outcomes for patients having total hip or knee arthroplasty: Protocol of a meta-analytic study from cohort studies
Authors: Lin Liang , Zhang Qing , Xu Min , Xiao Zhihong , Xu Guosong , Mei Zubing 
Published: January 2025

Background and purpose
Total hip arthroplasty (THA) and total knee arthroplasty (TKA) are widely performed surgeries for end-stage joint disease, yet the influence of depression and anxiety on postoperative outcomes remains unclear. This study aims to consolidate current evidence on the relationship between preoperative depression and/or anxiety disorders and postoperative outcomes in adult patients undergoing primary THA or TKA. Given the potential for these psychiatric conditions to affect recovery, pain management, and overall satisfaction, the results of this study are crucial to inform targeted perioperative interventions and improve patient-centered care.

Methods and analysis
We will search PubMed, Embase, Cochrane Library and PsycINFO from inception to the November 2024, adopting a comprehensive search strategy with no language restrictions. Eligible studies will include cohort studies evaluating adults with a diagnosis of depression and/or anxiety before THA or TKA compared to those without such disorders. Inclusion criteria will focus on preoperative psychiatric diagnoses, clearly defined postoperative outcomes (such as complications, functional recovery measures, pain, length of stay, and patient-reported outcomes). Risk of bias assessment will be performed using the Newcastle-Ottawa Scale. Meta-analysis will be conducted using a random-effects model to calculate pooled risk estimates and 95% confidence intervals for each outcome. Heterogeneity will be quantified with the I2 statistic, and a threshold of I2 > 50% will indicate substantial heterogeneity. Sources of heterogeneity will be explored via subgroup analyses or meta-regression if possible. Potential publication bias will be visually assessed using funnel plots and statistically tested using Egger’s test. Sensitivity analyses will be carried out to evaluate the robustness of the results for each outcome through leave-one-out procedure.

Discussion
This study will introduce a systematic and rigorous approach to synthesizing evidence from multiple cohorts, providing a comprehensive understanding of the impact of depression and anxiety on THA and TKA outcomes. The findings will guide clinicians in recognizing and managing mental health issues to optimize postoperative recovery and ultimately improve patient satisfaction and quality of life.

Study registration
Study registration: CRD42024500008.
Law and Economics Scholarship in Malaysia
Authors: Khong Dennis W. K. , Kong Erica X.W. 
Published: January 2025

Abstract
This article reports law and economics activities from Malaysia or by Malaysians. It surveys the resulting literature and identifies selected personalities who have contributed to advancing law and economics research in Malaysia. Other past activities, such as organising an online conference for the Asian Law and Economics Association in 2020, are recounted. It notes the extent to which Malaysian universities offer law and economics courses. The Malaysian government’s requirement for regulatory impact statements is the right direction to instill further interest in law and economics in government officers. Finally, some existing challenges and opportunities for future development are examined for future actions.
Sustainability as a business opportunity: a corpus-assisted discourse analysis of sustainable finance discourse
Authors: Nervino Esterina , Lan Ge , Cheung Joyce Oiwun 
Published: January 2025

Abstract
As sustainability becomes increasingly embedded in the practices of financial institutions worldwide, financial discourse is also adapting to reflect the new related societal values. This study aims to identify the discursive strategies employed by banks to frame their leadership in sustainability, present their sustainable products and services as business opportunities, and explore the development of dialogic relationships between banks and clients. Employing a corpus-assisted discourse analytical approach, a dataset of 2 million words comprised of sustainability-related reports publicly available on banks’ websites was analysed. The findings indicate a growing recognition of the significance of diversity and inclusion in senior leadership positions, with an observed increase in the representation of women. Moreover, banks position themselves as service providers and catalysts for transforming associated risks into business opportunities, leveraging various factors such as financial success, positive culture, and strong support for customers and employees. In terms of the bank-client relationship, the findings reveal a shift towards empowering clients and acknowledging their agency, encouraging them to make informed decisions and take charge of their financial well-being. This research sheds light on the evolving discursive practices within the realm of sustainable finance and provides valuable insights for both academia and the business sector.
Leveraging mutually shared knowledge through translanguaging in EMI classrooms: combining multimodal conversation analysis with interpretative phenomenological analysis
Authors: Tai Kevin W. H. , Li Linhong 
Published: January 2025

Abstract
Recent research has examined how teachers utilize translanguaging to tap into students’ out-of-school knowledge and students’ prior learnt content knowledge to scaffold students’ learning of new content knowledge. This study addresses a research gap by examining how teachers can maximize the utilization of mutually shared knowledge, which is not accessible to individuals outside the classroom community, through translanguaging to consolidate students’ content learning. The data is derived from a larger project conducted in Hong Kong secondary English-Medium-Instruction mathematics classrooms. Multimodal Conversation Analysis (MCA) is employed to analyse classroom interactions, triangulated by video-stimulated-recall interviews analysed with Interpretative Phenomenological Analysis (IPA). We argue that establishing a translanguaging space allows teachers to capitalize on the shared sociocultural knowledge intrinsic to classroom communities, which shapes content instruction and forges meaningful relationships with students. We also highlight the significance of combining MCA with IPA to gain a deeper understanding of specific translanguaging moments and the reasoning behind incorporating mutually shared sociocultural knowledge into classroom interactions, which cannot be attained solely through the description of interactional sequences.
Gene mining, recombinant expression and enzymatic characterization of N-acetylglucosamine deacetylase
Authors: Qiu Min , Dai Xiaohui , Hu Jiliang , Zhang Jianlong , Liu Qiang , Luan Jiabao , Zhou Qingmei , Xia Yu , Zhang Kunxiao , Liu Weiwei 
Published: January 2025

Abstract

Objectives
Glucosamine (GlcN) is an important bioactive substance that is widely used in medicine, dietary supplements, cosmetics, and other fields. The traditional method of producing GlcN is mainly through chitosan hydrolysis catalyzed by strong acid, but this process is usually accompanied by environmental pollution and high energy consumption. Therefore, the development of green and efficient production methods of glucosamine has become the focus of current research.

Methods
In this study, N-acetylglucosamine (GlcNAc) was used as the substrate to facilitate the enzymatic synthesis of GlcN by deacetylase. Four deacetylases (TkDAc, PkDAc, PpDAc and AbDAc) were selected from marine thermophilic microorganisms, and Escherichia coli (E. coli) was used as the host for recombinant expression.

Results
The soluble expression of PpDAc was poor, so several groups of solubilizing labels were tried, and the results showed that the soluble expression of recombinant plasmid ArsC-PpDAc carrying pro-solubilization labels was greatly improved.

Conclusions
The effects of temperature and pH on enzyme activity were investigated by single factor analysis. Kinetic parameters further revealed that ArsC-PpDAc exhibited the highest catalytic activity, with a Kcat/Km value of 7.29, and achieved a conversion rate of over 95 %. The condition of ArsC-PpDAc was optimized, and the results showed that ArsC-PpDAc showed good tolerance to organic solvents, and its catalytic activity was not significantly affected.