bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D‐printed nanocomposite denture base resin: The effect of incorporating TiO2 nanoparticles on the growth of Candida albicans
Authors: Altarazi Ahmed , Jadaan Layali , McBain Andrew J. , Haider Julfikar , Kushnerev Evgeny , Yates Julian M. , Alhotan Abdulaziz , Silikas Nick , Devlin Hugh 
Published: October 2023

AbstractPurposeTo develop a biocompatible denture base resin/TiO2 nanocomposite material with antifungal characteristics that is suitable for 3D‐printing denture bases.Materials and MethodsTiO2 nanoparticles (NPs) with a 0.10, 0.25, 0.50, and 0.75 weight percent (wt.%) were incorporated into a commercially available 3D‐printed resin material. The resulting nanocomposite material was analyzed using Lactate dehydrogenase (LDH) and AlamarBlue (AB) assays for biocompatibility testing with human gingival fibroblasts (HGF). The composite material was also tested for its antifungal efficacy against Candida albicans. Fourier transform infrared (FTIR) and Energy Dispersive X‐ray Spectroscopy (EDX) mapping were conducted to assess the surface coating and the dispersion of the NPs.ResultsLDH and AB assays confirmed the biocompatibility of the material showing cell proliferation at a rate of nearly 100% at day 10, with a cytotoxicity of less than 13% of the cells at day 10. The concentrations of 0.10, 0.25, and 0.50 wt.% caused a significant reduction (p < 0.05) in the number of candida cells attached to the surface of the specimens (p < 0.05), while 0.75 wt.% did not show any significant difference compared to the control (no TiO2 NPs) (p > 0.05). FTIR and EDX analysis confirmed the presence of TiO2 NPs within the nanocomposite material with a homogenous dispersion for 0.10 and 0.25 wt.% groups and an aggregation of the NPs within the material at higher concentrations.ConclusionThe addition of TiO2 NPs into 3D‐printed denture base resin proved to have an antifungal effect against Candida albicans. The resultant nanocomposite material was a biocompatible material with HGFs and was successfully used for 3D printing.
Influence of age, training, intraoral scanner, and software version on the scan accuracy of inexperienced operators
Authors: Zarauz Cristina , Pradíes Guillermo Jesus , Chebib Najla , Dönmez Mustafa Borga , Karasan Duygu , Sailer Irena 
Published: October 2023

AbstractPurposeTo evaluate the effect of operator age on the scan accuracy (trueness and precision) of inexperienced operators when compared with experienced operators, and how training, intraoral scanner (IOS), and software version affect scan accuracy.Material and MethodsThirty‐four operators were sorted into groups: G1 (operators <25 years old, no experience), G2 (operators >40 years old, no experience), and G3 (experienced IOS operators). They conducted partial‐arch scans before and after a 4‐session training with two IOSs (Trios 3 and True Definition) and two software versions. These scans were compared with the reference scans obtained from conventional impressions and a laboratory scanner (IScan D103i) to evaluate trueness (mean root mean square values) and precision (standard deviation of root mean square values) with a software program (Geomagic Control X). Kruskal‐Wallis and post‐hoc Dunn's tests were used to evaluate the effect of age on the scan accuracy of inexperienced groups when compared with experienced operators, while the effect of training, IOS, and software version on scan accuracy was evaluated with Wilcoxon or Mann‐Whitney U tests (α = 0.05).ResultsBefore training, G1 and G2 scans had similar accuracy (p ≥ 0.065). After training, G1 scans had higher accuracy when IOS data was pooled and had higher precision with TD (p ≤ 0.004). Training increased the scan accuracy (p < 0.001), while newer software increased the trueness of inexperienced operator scans (p = 0.015).ConclusionsAge affected the scan accuracy of inexperienced operators after training, indicating that extended training may be required for older operators. Training increased the scan accuracy, and newer software increased the trueness of inexperienced operator scans.
Using measurement alignment in research on adolescence involving multiple groups: A brief tutorial with R
Authors: Han Hyemin 
Published: October 2023

AbstractMeasurement alignment adjusts factor loadings and intercepts across different groups to achieve measurement invariance, which assumes the equal measurement model is validated across different groups. It should be achieved for validly conducting analysis and comparison in studies involving multiple groups, such as cross‐cultural or cross‐national studies. In this paper, I presented how to conduct measurement alignment with R. In addition to measurement alignment, I explained how to perform the Monte Carlo simulation to test the consistency and validity of alignment results and factor score calculation to facilitate further statistical analysis. A tutorial R code that implements all described procedures is freely shared via GitHub to inform readers who intend to use the alignment technique in their research projects.
Effect of dietary inclusion of mannan oligosaccharides on the efficacy of a novel hydrogen peroxide‐inactivated vaccine against Streptococcus agalactiae in Nile tilapia
Authors: Ramos‐Espinoza Fernando Carlos , Cueva‐Quiroz Victor Alexander , Alvarez‐Rubio Norquis Caled , Paganoti de Mello Nicoli , de Moraes Julieta Rodini Engrácia 
Published: October 2023

AbstractMannan oligosaccharides (MOS) have shown to stimulate immune response in different fish species, but the results may appear contradictory and have not been tested in conjunction with vaccination. We hypothesized that dietary MOS supplementation could enhance efficacy and immune responses after immunization with a novel hydrogen peroxide‐inactivated vaccine. Nile Tilapia, Oreochromis niloticus, fingerlings were distributed into four groups (supplemented or not, vaccinated or not) and were used to determine immune and hematological parameters and somatic indexes. For the bacterial challenge and vaccine efficacy test, fish were assigned to five experimental groups. Twenty‐eight days after the feeding trial and vaccination, the groups were challenged intraperitoneally and monitored for 21 days. The relative percent survival of the groups basal diet‐vaccinated and MOS diet‐vaccinated were 98.1% and 100.0%, respectively. The dietary inclusion of MOS after vaccination for 28 days had no influence on vaccine efficacy, which could be explained because of the high efficacy of the vaccine itself. Therefore, we suggest that the contribution of MOS supplementation to immune responses after vaccination in fish would be greater for vaccines with lower efficacy, or when tested for heterologous challenges or for crossed protection.
Identification and validation of microtubule depolymerizing agent, CYT997, as a potential drug candidate for hepatocellular carcinoma
Authors: Ahmad Faiz , Ma Li , Wei Wei , Liu Yi , Hakim Isaac , Daugherty Aaron , Mujahid Sana , Radin Andrew A. , Chua Mei‐Sze , So Samuel 
Published: October 2023

AbstractBackground and AimsHepatocellular carcinoma (HCC) is a typically fatal malignancy with limited treatment options and poor survival rates, despite recent FDA approvals of newer treatment options. We aim to address this unmet need by using a proprietary computational drug discovery platform that identifies drug candidates with the potential to advance rapidly and successfully through preclinical studies.MethodsWe generated an in silico model of HCC biology to identify the top 10 small molecules with predicted efficacy. The most promising candidate, CYT997, was tested for its in vitro effects on cell viability and cell death, colony formation, cell cycle changes, and cell migration/invasion in HCC cells. We used an HCC patient‐derived xenograft (PDX) mouse model to assess its in vivo efficacy.ResultsCYT997 was significantly more cytotoxic against HCC cells than against primary human hepatocytes, and sensitized HCC cells to sorafenib. It arrested cell cycle at the G2/M phase with associated up‐regulations of p21, p‐MEK1/2, p‐ERK, and down‐regulation of cyclin B1. Cell apoptosis and senescence‐like morphology were also observed. CYT997 inhibited HCC cell migration and invasion, and down‐regulated the expressions of acetylated tubulins, β‐tubulin, glypican‐3 (GPC3), β‐catenin, and c‐Myc. In vivo, CYT997 (20 mg/kg, three times weekly by oral gavage) significantly inhibited PDX growth, while being non‐toxic to mice. Immunohistochemistry confirmed the down‐regulation of GPC3, c‐Myc, and Ki‐67, supporting its anti‐proliferative effect.ConclusionCYT997 is a potentially efficacious and non‐toxic drug candidate for HCC therapy. Its ability to down‐regulate GPC3, β‐catenin, and c‐Myc highlights a novel mechanism of action.
The use of photovoice in nursing education: A scoping review
Authors: Randall Paige S. , De Gagne Jennie C. , Yamane Sandra S. , Lee Eunhee 
Published: October 2023

AbstractGiven the variety of uses of photovoice in nursing education, the importance of clarifying and describing this method is apparent. This scoping review aimed to explore the evidence on the use of photovoice in nursing education, describe the state of knowledge in this area, and identify knowledge gaps within the existing literature. A comprehensive search was performed in seven databases including MEDLINE, CINAHL, Embase, and Education Full Text. Three gray literature databases (ProQuest Dissertations and Theses Global database, EThOS, and PapersFirst) were also searched. This scoping review was conducted with reference to the JBI scoping review guidelines and reported using the PRISMA‐ScR statement. A total of 22 studies published between 2001 and 2022 were included in this scoping review. The findings indicate that photovoice is used globally in nursing education to teach in various courses and content areas within online, hybrid, and in‐person environments. Despite the heterogeneity within the definition of photovoice and associated pedagogical strategies, the results of this scoping review suggest that photovoice can serve as an educational tool for nursing students.
Association between screen time and depressive symptoms in a sample of Chinese medical students: Mediator role of empathy
Authors: Tang Zhengyu , Xiang Hongshu , Geng Yiran , Liao Xiting , Zhang Ming , Zhang Tianyang 
Published: October 2023

AbstractMedical students are at high risk of psychological subhealth under heavy stress with increasing screen time. This study aimed to explore the association between screen time and depressive symptoms and determine empathy as a mediating factor. In this cross‐sectional study, a total of 945 medical students were surveyed, and 924 medical students were ultimately included after standard exclusion criteria. They reported their daily screen time and completed the Chinese version of the Jefferson Scale of Empathy‐Student Version (JSE‐S) and the Self‐Rating Depression Scale (SDS). t tests and analysis of variance showed a significant difference in empathy and depressive symptoms by sex, stage, and screen time. The correlation analysis revealed that both affective and cognitive empathy have inverse associations with depressive symptoms. The mediation model confirmed that cognitive empathy played a positive mediating role between screen time and depressive symptoms, reducing the impact of screen time on depressive symptoms. Our study may add empirical evidence to prevent and intervene in depressive symptoms. These findings call for considering controlling screen time and enhancing cognitive empathy as interventions for medical students' depressive symptoms.
Glutamate decarboxylase‐1 is essential for efficient acclimation of Arabidopsis thaliana to nutritional phosphorus deprivation
Authors: Benidickson Kirsten H. , Raytek Lee Marie , Hoover Gordon J. , Flaherty Edward J. , Shelp Barry J. , Snedden Wayne A. , Plaxton William C. 
Published: October 2023

Summary
Glutamate decarboxylase (GAD) is a Ca2+‐calmodulin‐activated, cytosolic enzyme that produces γ‐aminobutyrate (GABA) as the committed step of the GABA shunt. This pathway bypasses the 2‐oxoglutarate to succinate reactions of the tricarboxylic acid (TCA) cycle. GABA also accumulates during many plant stresses.
We tested the hypothesis that AtGAD1 (At5G17330) facilitates Arabidopsis acclimation to Pi deprivation. Quantitative RT‐PCR and immunoblotting revealed that AtGAD1 transcript and protein expression is primarily root‐specific, but inducible at lower levels in shoots of Pi‐deprived (−Pi) plants.
Pi deprivation reduced levels of the 2‐oxoglutarate dehydrogenase (2‐OGDH) cofactor thiamine diphosphate (ThDP) in shoots and roots by > 50%. Growth of −Pi atgad1 T‐DNA mutants was significantly attenuated relative to wild‐type plants. This was accompanied by: (i) an > 60% increase in shoot and root GABA levels of −Pi wild‐type, but not atgad1 plants, and (ii) markedly elevated anthocyanin and reduced free and total Pi levels in leaves of −Pi atgad1 plants. Treatment with 10 mM GABA reversed the deleterious development of −Pi atgad1 plants.
Our results indicate that AtGAD1 mediates GABA shunt upregulation during Pi deprivation. This bypass is hypothesized to circumvent ThDP‐limited 2‐OGDH activity to facilitate TCA cycle flux and respiration by −Pi Arabidopsis.
Analysis of oral lichen planus severity on micro‐RNA linked with malignant transformation risks
Authors: Polizzi Alessandro , Santonocito Simona , Distefano Alfio , De Pasquale Rocco , Alibrandi Angela , Alanazi Amer M. , Li Volti Giovanni , Isola Gaetano 
Published: October 2023

AbstractObjectiveThe present study evaluated the oral tissue expression of micro‐RNA (miRNAs) linked to the potential malignant evolution of oral lichen planus (OLP). Furthermore, the correlation between OLP severity and miRNAs expression was assessed, and possible predictors of miRNAs in OLP patients were identified.MethodsThe present study enrolled 41 patients with OLP (median age 58 years) and 42 healthy controls (median age 59 years). In each patient, miRNA levels (miR‐7a‐3p,‐7a2‐3p,‐7a‐5p,‐21‐3p,‐21‐5p,‐100‐3p,‐100‐5p,‐125b‐2‐3p,‐125b‐5p,‐200b‐3p,‐200b‐5p) were assessed and analyzed through reverse transcription polymerase chain reaction. Clinical parameters and the eventual presence of OLP symptoms, signs, and disease severity scores in each patient were reported using an anamnestic questionnaire.ResultsIn comparison with healthy controls, OLP patients showed significantly higher miR‐7a‐3p,‐7a‐2‐3p,‐21‐3p, miR‐21‐5p and miR‐100‐5p levels (p < 0.05) and significantly lower miR‐125b‐2‐3p,‐125b‐5p,‐200b‐3p, and ‐200b‐5p levels (p < 0.05). Furthermore, OLP symptoms and signs and disease severity scores were significantly correlated and were also predictors of all analyzed miRNAs (p < 0.05).ConclusionsIn comparison with healthy subjects, OLP patients exhibited unbalanced oral miRNAs expression linked to the risk of potential malignant evolution of OLP. Furthermore, some miRNAs were correlated with OLP extent and were significant predictors of OLP symptoms, signs, and disease severity scores.
Perioperative analgesic efficacy and adverse events of fentanyl in dentistry: A systematic review
Authors: Ardila Carlos M. , Jiménez‐Arbeláez Gustavo A. , Vivares‐Builes Annie Marcela 
Published: October 2023

AbstractObjectivesTo assess the efficacy and adverse events linked to the utilization of fentanyl for perioperative pain management in dentistry.MethodsThis systematic review of randomized clinical trials (RCTs) adhered to the PRISMA guidelines and incorporated various databases.ResultsEleven RCTs studying 674 patients were analyzed. Perioperative pain was predominantly evaluated in patients undergoing surgery for impacted molars, although some studies also included patients with other conditions such as oral submucous fibrosis, maxillary cancer, bony temporomandibular joint ankylosis, irreversible pulpitis, among others. Combined with dexmedetomidine, fentanyl produced enhanced analgesic effects. It demonstrated comparable efficacy when compared to nefopam and nalbuphine. Both intranasal and intravenous administration routes proved equally effective. In four RCTs, the transdermal fentanyl patch outperformed the control group, except in the clinical trial where it was compared to ropivacaine. The main adverse events associated with the use of fentanyl included nausea, vomiting, drowsiness, delirium, and respiratory depression; however, they were like those reported in the comparison groups.ConclusionsWhile fentanyl demonstrated satisfactory perioperative analgesic efficacy, there were other alternatives that displayed better or comparable outcomes. Due to the risks and potential for misuse of fentanyl, these alternatives must be considered although adverse events were also reported.
A retrospective review of elevated lead impedances in impedance‐dependent magnetic resonance‐conditional spinal cord stimulation devices
Authors: Mullins Cormac Francis , Harris Stephany , Pang David 
Published: October 2023

AbstractObjectivesAdvances in Spinal cord stimulation (SCS) device technology in recent years have led to the development of SCS systems that are magnetic resonance imaging (MRI)‐conditional, most of which are dependent on normal lead impedances. The objective of this study was to retrospectively analyze the rate of elevated lead impedance in these devices to determine the rate of failure of MR‐conditional modes.Materials and MethodsThis was a single‐center, retrospective, chart‐based review conducted during a five‐year period. Patients were included if they had been implanted with an impedance‐dependent MR‐conditional SCS and had a documented impedance check at least 6 months after implantation. A Kaplan–Meier survival analysis was performed to map the survival of MR‐conditionality over time.ResultsThere were 363 cases included between 2015 and 2020, which corresponded to a total of 602 SCS leads. Nevro was the most common manufacturer (67.8%), followed by Boston Scientific (22.3%) and Abbott (9.9%). The average overall follow‐up time was 2.25 years. Overall, 67 (18.5%) of patients had lead impedances over 10,000 Ω at follow‐up with a total of 186 electrode contacts (3.9%). Leads most commonly had either one (40%), two (22%) or three (12%) electrode contacts out of range. Risk of failure of lead impedances increased by 35.4% with each successive year to a peak of 43% of all leads by year 5. Mean overall survival time of normal lead impedances was 4.77 years (CI 4.40–5.13). There was no statistically significant difference in mean overall survival time between Abbott (M = 4.0 years, SD = 1.25), Boston Scientific (M = 4.64 years, SD = 1.75) and Nevro (M = 4.80 years, SD = 3.28), χ2 (2, N = 358) = 1.511, p = 0.47; however, Abbott leads had a greater total number of failed impedance contacts (50/568, 8.8%), in comparison to Nevro (124/3064, 4.0%), χ2 (1, N = 3630) = 23.76, p < 0.00001, at a similar follow‐up time.ConclusionThis retrospective study identified elevated impedances in 18.5% of MR‐conditional SCS devices at an average of 2.25 years follow‐up resulting in loss of MR‐conditionality and a mean overall lead survival time of 4.77 years for normal lead impedance.
What can preemption do?
Authors: Avnur Yuval , Obiegbu Chigozie 
Published: October 2023

AbstractEvidential Preemption occurs when a speaker asserts something of the form “Others will tell you Q, but I say P,” where P and Q are incompatible in some salient way. Typically, the aim of this maneuver is to get the audience to accept P despite contrary testimony of others, who might otherwise be trusted on the matter. Phenomena such as echo chambers, conspiracy theories, and other political speech of interest to epistemologists today, all commonly involve evidential preemption, so the question arises: What effect, if any, does evidential preemption have on the audience's epistemic situation? In a widely discussed paper, Begby (Philosophy and Phenomenological Research, 2021, 102, 515) argues that evidential preemption can change the audience's epistemic situation in such a way that future testimony that Q, which would otherwise have been persuasive, can be rationally discounted to a significant degree. If so, evidential preemption is not a mere rhetorical flourish, but rather results in a rationally insulated belief that P. Since evidential preemption is a common feature of echo chambers and conspiracy theories, this would be a disturbing result. We bring good news: it is not so, at least not in the way Begby suggests. If evidential preemptions can change one's epistemic situation in a worrying way, it is a mystery how.
Towards a Fregean psycholinguistics
Authors: Sander Thorsten 
Published: October 2023

AbstractThis paper is partly exegetical, partly systematic. I argue that Frege's account of what he called “colouring” contains some important insights on how communication is related to mental states such as mental images or emotions. I also show that the Fregean perspective is supported by current research in psycholinguistics and that a full understanding of some linguistic phenomena that scholars have accounted for in terms of either semantics or pragmatics need involve psycholinguistic elements.
Toward a better understanding of local service provision: Implications for studying the determinants of production choice
Authors: Lamothe Scott , Lamothe Meeyoung 
Published: October 2023

AbstractResearchers studying local governance, especially alternative service delivery arrangements, have long relied on the ICMA ASD survey to examine the scope and nature of service provision and production at the local level. Building upon Lamothe et al. (Public Administration Review, 2018, 78: 613) findings that raise questions concerning the accuracy of the ASD survey and resulting misconceptions about service provision, this paper refines how provision can be conceptualized and measured to promote a better understanding of local governance. Utilizing a unique survey design that incorporates, but also extends the ASD format, we demonstrate that service provision is multifaceted, and jurisdictions participate in varying degrees of provision activities based on the sector of the producing entity. Our findings highlight the importance of properly accounting for provision activities and call into question the efficacy of the conventional contracting framework as the primary theoretical underpinning of most studies of local service delivery arrangements.