bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stainability and translucency of potassium aluminum sulfate applied computer‐aided design and computer‐aided manufacturing materials after coffee thermocycling
Authors: Sasany Rafat , Donmez Mustafa Borga , de Paula Marcella Silva , Kahveci Çiğdem , Ceylan Gözlem , Yilmaz Burak , Çakmak Gülce 
Published: October 2023

AbstractObjectiveThe purpose of this in vitro study was to evaluate the effect of potassium aluminum sulfate (alum) application on the stainability and translucency of computer‐aided design and computer‐aided manufacturing (CAD‐CAM) materials after coffee thermocycling (CTC).Materials and MethodsDisk‐shaped specimens (Ø10 × 1 mm; N = 200) were fabricated by using additively (Crowntec [CT] and Varseo Smile Crown Plus [VS]) and subtractively manufactured (Brilliant Crios [RCR], CEREC Block [FC], and Vita Enamic [VE]) CAD‐CAM materials and polished. All specimens were randomly divided into two groups as alum applied and control (n = 10). All specimens were then subjected to CTC (10,000 cycles at 5–55°C) and color coordinates were measured at each time interval. Color differences (ΔE00) and relative translucency parameters (RTPs) were calculated and the data were statistically analyzed (a = 0.05).ResultsAmong tested time intervals, alum applied specimens had their lowest ΔE00 after alum application (p ≤ 0.006), except for FC (p = 0.177). In addition, alum applied RCR had lower ΔE00 values than its control specimens (p = 0.029). Alum applied specimens had their lowest RTP after CTC (p < 0.001) and alum application decreased the RTP of CT (p = 0.010). CTC reduced the RTP of all materials in control groups (p < 0.001). Alum applied CT had higher RTP than its control specimens (p = 0.049).ConclusionsAlum application's effect on color change varied depending on the material and alum mostly resulted in clinically acceptable changes in translucency. CTC led to unacceptable color and translucency changes based on previously reported threshold values.Clinical SignificanceOptical properties of CAD‐CAM materials and the sustainability of these properties over time is critical for longevity. Alum may improve the color stability of reinforced composite resin when subjected to long‐term coffee consumption.
Modelling 3D supramolecular structure from sparse single‐molecule localisation microscopy data
Authors: Curd Alistair , Cleasby Alexa , Baird Michelle , Peckham Michelle 
Published: October 2023

AbstractSingle‐molecule localisation microscopy (SMLM) has the potential to reveal the underlying organisation of specific molecules within supramolecular complexes and their conformations, which is not possible with conventional microscope resolution. However, the detection efficiency for fluorescent molecules in cells can be limited in SMLM, even to below 1% in thick and dense samples. Segmentation of individual complexes can also be challenging. To overcome these problems, we have developed a software package termed PERPL: Pattern Extraction from Relative Positions of Localisations. This software assesses the relative likelihoods of models for underlying patterns behind incomplete SMLM data, based on the relative positions of pairs of localisations. We review its principles and demonstrate its use on the 3D lattice of Z‐disk proteins in mammalian cardiomyocytes. We find known and novel features at ~20 nm with localisations of less than 1% of the target proteins, using mEos fluorescent protein constructs.
Achieving the minimum pain experience by buccal nerve and superficial cervical plexus blocks in radiofrequency treatment
Authors: Chen Xihua , Han Changzhu , Li Dan , Zhu Feiling , Huang Ying 
Published: October 2023

AbstractBackgroundThermage is a monopolar radiofrequency (RF). It has become an indispensable part of facial and body youthful methods. Although the current device is constantly improving in epidermal cooling techniques and even automatically measures the local impedance value, applying surface anesthesia can take some of the pain away caused by thermage, and the patient's severe pain in the jaw and neck areas is still difficult to resolve.MethodsThe author describes how he uses the combination of the buccal nerve block (BNB) and the superficial cervical plexus block (SCPB) to improve the comfort of the patient's face and neck treatment of patients. It can improve the quality, elasticity, and texture of the skin.ResultsAccording to the author's knowledge of oral and maxillofacial surgery, the combination of BNB and SCPB is applied to the neck and facial analgesia. Combining the BNB and SCPB effectively provides facial and neck anesthesia. The BNB in this technique provides pain relief to the facial skin in the mandibular area and SCPB offers pain relief to the skin of the neck. RF facial rejuvenation treatment often involves the junction of the face and neck to improve the contour of the mandibular margin. Therefore, Whether the RF treatment is aimed at the face or neck, or the treatment is performed simultaneously, we advocate the block both of the buccal nerve and the superficial cervical plexus nerves to achieve perfect analgesia. Still, the dosage of anesthetic medication for the nerve block can be adjusted according to the treatment area.ConclusionWe applied these two nerve blocks and their combination to improve skin laxity with RF therapy for the first time.
Educator Perspectives on Grief‐Sensitive Training During the COVID‐19 Pandemic in US Public Schools
Authors: Lively‐Endicott Hannah R. , Naimi Kiana , Hudson Sharon M. , Schonfeld David J. 
Published: October 2023

ABSTRACTBackgroundGrief and loss are common experiences for children and adolescents, particularly during the COVID‐19 pandemic. Educators feel unprepared to support grieving students due to lack of training. We studied educator experiences receiving grief‐sensitive training as part of the grief‐sensitive schools initiative (GSSI), which provides grief‐sensitive training, online video‐based and print resources, and a financial grant to schools and school districts for use in supporting grieving students.MethodsFourteen New York and Florida educators who received GSSI training participated in small focus groups or semi‐structured interviews on their experiences receiving GSSI training and supporting grieving students during the pandemic. Transcripts were analyzed using grounded‐theory analysis.ResultsEmergent themes included increased confidence engaging grieving students, the desire for recurring trainings, the value of receiving training from an expert on pediatric grief and loss and the opportunity to ask questions, the need for grief‐sensitive training to reflect the cultural diversity of school communities, the unique losses experienced by students during the pandemic, and compassion fatigue and burnout in educators.Implications for School Health Policy, Practice, and EquityPolicymakers should recognize the effects of grief on students' learning and development and collaborate with educators to develop resources.ConclusionsEducators found GSSI training useful in supporting grieving students, particularly during the pandemic.
Association between voiding lower urinary tract symptoms and findings on dynamic magnetic resonance imaging with regard to pelvic organs and their supportive structures
Authors: Watanabe Shoutarou , Kinno Kurenai , Takeuchi Yasuharu , Sawada Yoshitomo , Sekido Noritoshi 
Published: October 2023

AbstractObjectivesPelvic organ prolapse (POP) causes voiding lower urinary tract symptoms (vLUTS). In the present study, we investigated the association between vLUTS and pelvic organ mobility (POM), including relevant supportive structures, on dynamic magnetic resonance imaging (dMRI).MethodsWe included 118 patients who had POP of stage II or less before straining and stage III or more when straining during dMRI. The presence of vLUTS and overactive bladder (OAB) was determined by a voiding subscore of the International Prostate Symptom Score (vIPSS) ≥5 and the OAB symptom score, respectively. POM was measured by dMRI before and during straining, and patients with and without vLUTS as well as patients with and without vLUTS and/or OAB were compared. p < .05 was considered to be statistically significant.ResultsAccording to vIPSS, 42 patients (35.6%) had vLUTS. On dMRI, patients with vLUTS showed a significantly more ventral position and/or movement of the bladder and cervix. Moreover, patients with vLUTS and OAB had significantly more ventral movement of the uterine cervix and a larger strain on imaginary uterosacral and cardinal ligaments than those without these symptoms. In addition, patients with vLUTS and OAB had significantly higher vIPSS than those with vLUTS alone.ConclusionsvLUTS may be associated with the proximity of the bladder and cervix to the pubic bone and consequent compression of the urethra by the prolapsed organs. vLUTS with OAB might indicate more advanced lower urinary tract dysfunction than vLUTS alone.
Bilingual heritage language learning in China: Applying the Douglas Fir Group's transdisciplinary framework
Authors: Lu Yuan , Ke Chuanren 
Published: October 2023

AbstractThrough the application of the Douglas Fir Group's transdisciplinary framework, this study investigated how three levels of mutually dependent influence (i.e., the micro level of social action and interaction, meso level of sociocultural institutions and communities, and macro level of ideological structures) operate across time and space to shape a bilingual heritage language learner's study‐abroad (SA) experiences. Data were collected from standardized proficiency tests; language contact profiles; pre‐ and midprogram surveys; and interviews with the learner, classroom instructors, tutor–roommates, host family members, and the director of the SA program. The transdisciplinary framework enabled this study to expand its research focus and analytical scope, demonstrating that language learning was mediated by the three levels of mutually dependent influence in a dynamic and complex manner. This study has generated a richer understanding of multilayered complexity in heritage language development, advanced bilingual performance and proficiency, and bilingualism in language education and society.
Oral squamous cell carcinomas and oral potentially malignant disorders: A Latin American study
Authors: Gilligan Gerardo , Panico René , Lazos Jerónimo , Morelatto Rosana , Belardinelli Paola , Criscuolo Maria Ines , Bolesina Nicolas , Molina Ávila Ignacio , Pimentel Solá Juan Martin , Rivarola Emilce , Morgante Maria Jimena , Ingrassia Tonelli Maria Eugenia , Gimenez Javier , Doratti Pablo , Werner Laura Cecilia , Prado‐Ribeiro Ana Carolina , Brandão Thais Bianca , Vechiato‐Filho Aljomar Jose , Cuzzullin Maria Claudia , Saldivia‐Siracusa Cristina , Lopes Marcio Ajudarte , Agostini Michelle , Romañach Mario José , Abrahão Aline Corrêa , Fonsêca Thamyres Campos , Kaminagakura Estela , Anbinder Ana Lia , Santos Alves Kamilla , de Almeida Lança Maria Leticia , Sacsaquispe‐Contreras Sonia , Rodriguez Ibazetta Karem , Gerber‐Mora Roberto , Aranda Romo Saray , Tejeda Nava Francisco Javier , Cordero‐Torres Karina , Moreno Silva Ricardo , Gonzalez‐Arriagada Wilfredo , Toro Raiza , Silveira Felipe Martins , Gonzalez Natalia , Vargas Pablo Agustin , Santos‐Silva Alan Roger , Bologna‐Molina Ronell , Villarroel‐Dorrego Mariana , Martinez Benjamin , Piemonte Eduardo 
Published: October 2023

AbstractObjectiveTo determine the frequency of oral squamous cell carcinoma (OSCC) associated or not with oral potentially malignant disorders (OPMD), and the epidemiological profile and traditional risk factors in Latin America.MethodsA retrospective observational study was conducted in 17 Latin American centres. There were included cases of OSCC, analysing age, gender, OSCC and their association with previous OPMD. Clinicopathological variables were retrieved. The condition of sequential‐OSCC versus OSCC‐de novo (OSCC‐dn) was analysed concerning the aforementioned variables. Quantitative variables were analysed using Student's t‐test, and qualitative variables with chi‐square.ResultsIn total, 2705 OSCC were included with a mean age of 62.8 years old. 55.8% were men. 53.75% of the patients were smokers and 38% were common drinkers. The lateral tongue border was the most affected site (24.65%). There were regional variations in OPMD, being leukoplakia the most frequent. Of the overall 2705 OSCC cases, 81.4% corresponded to OSCC‐dn, while s‐OSCC were 18.6%. Regarding lip vermillion SCC, 35.7% corresponded to de novo lip SCC and 64.3% were associated with previous OPMD.ConclusionsIn Latin America, OSCC‐dn seems to be more frequent with regional variations of some clinical and histopathological features. Further prospective studies are needed to analyse this phenomenon.
Differential heat‐response characteristics of two plastid isoforms of triose phosphate isomerase in tomato
Authors: Chen Chong , Zhang Meng , Ma Xiaocui , Meng Qingwei , Zhuang Kunyang 
Published: October 2023

SummaryHeat stress causes dysfunction of the carbon‐assimilation metabolism. As a member of Calvin–Benson–Bassham (CBB) cycle, the chloroplast triose phosphate isomerases (TPI) catalyse the interconversion of glyceraldehyde 3‐phosphate (GAP) and dihydroxyacetone phosphate (DHAP). The tomato (Solanum lycopersicum) genome contains two individual SlTPI genes, Solyc10g054870 and Solyc01g111120, which encode the chloroplast‐located proteins SlTPI1 and SlTPI2, respectively. The tpi1 and tpi2 single mutants had no visible phenotypes, but the leaves of their double mutant lines tpi1tpi2 had obviously reduced TPI activity and displayed chlorotic variegation, dysplasic chloroplasts and lower carbon‐assimilation efficiency. In addition to altering carbon metabolism, proteomic data showed that the loss of both SlTPI1 and SlTPI2 severely affected photosystem proteins, reducing photosynthetic capacity. None of these phenotypes was evident in the tpi1 or tpi2 single mutants, suggesting that SlTPI1 and SlTPI2 are functionally redundant. However, the two proteins differed in their responses to heat stress; the protein encoded by the heat‐induced SlTPI2 showed a higher level of thermotolerance than that encoded by the heat‐suppressed SlTPI1. Notably, heat‐induced transcription factors, SlWRKY21 and SlHSFA2/7, which negatively regulated SlTPI1 expression and positively regulated SlTPI2 expression, respectively. Our findings thus reveal that SlTPI1 and SlTPI2 have different thermostabilities and expression patterns in response to heat stress, which have the potential to be applied in thermotolerance strategies in crops.
The treatable traits approach to adults with obstructive airways disease in primary and secondary care
Authors: Thomas Mike , Beasley Richard 
Published: October 2023

AbstractThe treatable traits approach is based on the recognition that the different clinical phenotypes of asthma and chronic obstructive airways disease (COPD) are a heterogeneous group of conditions with different underlying mechanisms and clinical manifestations, and that the identification and treatment of the specific clinical features or traits facilitates a personalised approach to management. Fundamentally, it recognises two important concepts. Firstly, that treatment for obstructive lung disease can achieve better outcomes if guided by specific clinical characteristics. Secondly, that in patients with a diagnosis of asthma, and/or COPD, poor respiratory health may also be due to numerous overlapping disorders that can present with symptoms that may be indistinguishable from asthma and/or COPD, comorbidities that might require treatment in their own right, and lifestyle or environmental factors that, if addressed, might lead to better control rather than simply increasing airways directed treatment. While these concepts are well accepted, how best to implement this personalised medicine approach in primary and secondary care within existing resource constraints remains uncertain. In this review, we consider the evidence base for this management approach and propose that the priority now is to assess different prototype templates for the identification and management of treatable traits in both asthma and COPD, in primary, secondary and tertiary care, to provide the evidence that will guide their use in clinical practice in different health care systems.
Research on the impact of waterfront resources on rural leisure environment, economic development, and the awareness of physical and mental health of the elderly
Authors: Lin Hsiao‐Hsien , Lin Tzu‐Yun , Lin Jan‐Wei , Lo Chih‐Cheng , Li Qi‐Yuan , Lin Chao‐Yen , Lin Jao‐Chuan , Li Cheng‐Ping 
Published: October 2023

AbstractThis study aimed to analyse the impact of waterfront resources on the rural leisure environment, economic development, and physical and mental health awareness of the elderly. This study adopted a mixed research method. First, a questionnaire was designed, and 580 questionnaires were analysed using SPSS 26.0 statistical software with a basic statistical test, Pearson correlation coefficient. Then, we used semi‐structured interviews to collect the opinions of six respondents. Finally, the data were compiled and discussed by multivariate check analysis. The study found that although coastal resources can promote the rural economy, maintain community culture, build a comfortable living environment for the elderly and promote health and well‐being, they still affect the physical and mental health and well‐being of the elderly because of serious pollution, damage to the community environment and ecology, high prices and few leisure facilities. Therefore, reducing pollution, stabilizing prices, maintaining coastal biodiversity, controlling the consumption mechanism and providing a safe leisure environment will help promote the physical and mental health of the elderly, increase their enthusiasm and self‐confidence, and increase their sense of well‐being.
Community involvement in addressing the antibiotic crisis
Authors: Kolokithas Angelo , Merkel Brian , Hunnicutt David , Fenzl Lucy , Petersen Matthew 
Published: October 2023

ABSTRACT
Increasing student interest and success in STEM education is a top priority for many postsecondary educational institutions. One well-documented approach to both priorities is to have students participate in a Course Undergraduate Research Experience (CURE). Faculty from several technical colleges and universities in Wisconsin teamed up with the Tiny Earth organization to offer a CURE to address the search for new antibiotics. Students enrolled in undergraduate microbiology courses engaged in research and participated in community outreach. To involve the community, faculty from various institutions joined an NFL team, the Green Bay Packers, and created the Tiny Earth in Titletown symposium. Here, students presented their work via scientific posters, to community and industry members, and networked with other scientists from around the region. The Tiny Earth in Titletown symposium started in 2018, was held again in 2019, and returned in 2022 following a 2-year hiatus due to the COVID-19 pandemic. Record attendance in 2022 suggests that community outreach and education may be helping restore trust in science that was lost during the pandemic.
Molecular mechanism of the interaction between
Megalocytivirus
-induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells
Authors: He Jian-hui , Shen Wenjie , Han Deyu , Yan Muting , Luo Mengting , Deng Hengwei , Weng Shaoping , He Jianguo , Xu Xiaopeng 
Published: October 2023

ABSTRACT

The
Megalocytivirus
genus, family Iridoviridae, is a group of large DNA viruses that pose significant threats to marine and freshwater fishes. In tissues, the
Megalocytivirus
-infected cells are attached by a layer of lymphatic endothelial cells (LECs), constituting a unique histopathological phenomenon.
Megalocytivirus
induces the formation of an extracellular structure on the surface of infected cells that is functionally similar to the host basement membrane (BM), known as the virus-mock basement membrane (VMBM). VMBM is composed of host BM component nidogen-1 and viral proteins VP23R and VP08R and specifically provides adhesive support for LECs. In the current study, the viral protein VP33L was identified as a novel component of VMBM, and the way it participates in VMBM assembly was investigated. The interactions between virus-encoded VMBM components and LEC-specific markers lymphatic vessel endothelial receptor-1 (LYVE-1) and vascular endothelial growth factor receptor 3 (VEGFR-3), which contribute to the selective adhesion of LECs on VMBM, were further analyzed. Moreover, the viral components of VMBM could regulate the proliferation and migration of LECs via the VEGFR-3 signaling, which partially explains the origin of LECs attached to infected cells. This study is important for revealing the pathogenesis of
Megalocytivirus
and potentially provides reference clues for exploring the interaction mechanism between endothelial cells and extracellular matrix in animals.

IMPORTANCE

Viruses are able to mimic the physiological or pathological mechanism of the host to favor their infection and replication. Virus-mock basement membrane (VMBM) is a
Megalocytivirus
-induced extracellular structure formed on the surface of infected cells and structurally and functionally mimics the basement membrane of the host. VMBM provides specific support for lymphatic endothelial cells (LECs) rather than blood endothelial cells to adhere to the surface of infected cells, which constitutes a unique phenomenon of
Megalocytivirus
infection. Here, the structure of VMBM and the interactions between VMBM components and LECs have been analyzed at the molecular level. The regulatory effect of VMBM components on the proliferation and migration of LECs has also been explored. This study helps to understand the mechanism of LEC-specific attachment to VMBM and to address the issue of where the LECs come from in the context of
Megalocytivirus
infection.