bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening of two efficient phosphorus-solubilizing bacteria, strain X42 (Bacillus_A_bombysepticus) and strain G62 (Bacillus velezensis) for their ability to promote Gan Nan navel orange growth
Authors: Zhao Wenyuan , Peng Tao , Cao Haojie , Huang Huimin , Yu Shuijing 
Published: February 2025

Abstract

Background
Due to inadequacies in the current management practices of navel orange orchards in southern Jiangxi, there is a deficiency in phosphorus content and a decline in overall soil quality. Therefore, developing microbial formulations that increase soil fertility while meeting green ecological standards is highly important. Rhizobacteria promote plant growth through various mechanisms, and given the critical role of phosphorus in plant growth and development, the development and application of such microbial agents offer an effective approach to address the aforementioned issues.

Results
This study screened two strains of bacteria with high phosphate solubilization capabilities from the roots of navel oranges in southern Jiangxi. These strains were inoculated into potted plants to investigate their potential to promote plant growth. A comparison of the growth indicators of the experimental and control groups, as well as the enzyme activity indicators of navel orange leaves, revealed that both strains exhibited good growth-promoting effects. Furthermore, whole-genome sequencing of the two strains was conducted, and by comparing data from 31 housekeeping genes, strain X42 was preliminarily identified as Bacillus bombysepticus, and strain G62 was identified as Bacillus velezensis. The comparison also revealed the presence of phosphate solubilization-encoding genes in both strains, with strain G62 lacking the genes for phytate mineralization and inorganic phosphorus dissolution, which may prevent it from utilizing additional organic phosphorus sources.

Conclusion
This study not only confirms the positive impact of two highly efficient phosphate-solubilizing Bacillus strains on the growth of navel oranges in southern Jiangxi but also deepens the understanding of the genetic basis of phosphate-solubilizing traits through whole-genome analysis. These findings are highly important for the development of biofertilizers and their application in sustainable agriculture, especially in terms of improving soil quality and increasing crop yields.
Improving multi-talker binaural DOA estimation by combining periodicity and spatial features in convolutional neural networks
Authors: Varzandeh Reza , Doclo Simon , Hohmann Volker 
Published: February 2025

Abstract
Deep neural network-based direction of arrival (DOA) estimation systems often rely on spatial features as input to learn a mapping for estimating the DOA of multiple talkers. Aiming to improve the accuracy of multi-talker DOA estimation for binaural hearing aids with a known number of active talkers, we investigate the usage of periodicity features as a footprint of speech signals in combination with spatial features as input to a convolutional neural network (CNN). In particular, we propose a multi-talker DOA estimation system employing a two-stage CNN architecture that utilizes cross-power spectrum (CPS) phase as spatial features and an auditory-inspired periodicity feature called periodicity degree (PD) as spectral features. The two-stage CNN incorporates a PD feature reduction stage prior to the joint processing of PD and CPS phase features. We investigate different design choices for the CNN architecture, including varying temporal reduction strategies and spectro-temporal filtering approaches. The performance of the proposed system is evaluated in static source scenarios with 2–3 talkers in two reverberant environments under varying signal-to-noise ratios using recorded background noises. To evaluate the benefit of combining PD features with CPS phase features, we consider baseline systems that utilize either only CPS phase features or combine CPS phase and magnitude spectrogram features. Results show that combining PD and CPS phase features in the proposed system consistently improves DOA estimation accuracy across all conditions, outperforming the two baseline systems. Additionally, the PD feature reduction stage in the proposed system improves DOA estimation accuracy while significantly reducing computational complexity compared to a baseline system without this stage, demonstrating its effectiveness for multi-talker DOA estimation.
Medication for opioid use disorder service delivery in carceral facilities: update and summary report
Authors: Berk Justin , South Anna-Maria , Martin Megan , James Michael-Evans , Miller Cameron , Haber Lawrence , Rich Josiah 
Published: February 2025

Abstract
The opioid overdose crisis intersects critically with the criminal legal system where individuals with opioid use disorder (are significantly overrepresented. Subsequently, incarceration increases the risk of opioid overdose due to reduced tolerance, interrupted social supports, and limited access to treatment. Medications for opioid use disorder (MOUD), such as methadone, buprenorphine, and naltrexone, have been shown to reduce opioid-related mortality and improve outcomes for those in carceral settings. Despite this, access to MOUD in jails and prisons remains limited due to stigma, concerns about medication diversion, and logistical challenges. This paper reviews the current state of knowledge on MOUD in carceral settings, summarizing the prevalence of treatment programs, the role of novel formulations like injectable buprenorphine, and barriers to implementation. It also explores the continuum of care, emphasizing the importance of initiating MOUD during incarceration and ensuring continuation upon release to prevent treatment gaps. Recent policy changes, such as Sect. 1115 Medicaid waivers, offer promising avenues for expanding access, but retention in treatment and post-release outcomes remain significant challenges. The paper provides a comprehensive overview of existing literature and updates on MOUD service delivery, including the impact of recent policy shifts and research on outcomes such as recidivism and health improvement. It concludes by identifying key areas for future research, including strategies to improve treatment retention, address systemic barriers through criminal justice reform, and enhance care coordination during the transition from incarceration to the community.
Civic and citizenship education, global citizenship education, and education for sustainable development: an analysis of their integrated conceptualization and measurement in the international civic and citizenship education study (ICCS) 2016 and 2022
Authors: Damiani Valeria , Fraillon Julian 
Published: February 2025

Abstract
Globalization and its impact on contemporary societies have gained new impetus with the notions of global citizenship education (GCED) and education for sustainable development (ESD), considered, together with civic and citizenship education (CCE), as a means for promoting students’ engagement in global/local issues and providing them with the awareness and skills to develop a deeper understanding of, and response to, contemporary issues. Research has shown that GCED and ESD, intended as two interrelated and intersecting fields, have strong associations with CCE and how citizenship is conceived in the post-national and global era. This paper aims to examine the issue of GCED/ESD achievement measurement within the field of civic and citizenship education. Combining analysis of the assessment frameworks and of data from the IEA ICCS (2016, 2022) achievement test (the civic knowledge test), it discusses the integration of the conceptualization and measurement of student achievement in civic knowledge with an ex-post operationalization and measurement of student achievement in concepts and content associated with GCED and ESD (for ICCS 2016) and subsequently analyses the associations between test items related to the GCED/ESD domain and those related to “the other CCE” domain in ICCS 2022. Results highlighted the progressive relevance of GCED/ESD in the ICCS studies and the blurred boundaries between GCED/ESD and CCE both in ICCS 2016 and 2022. In disentangling the implicit, growing interrelationship among these areas (theoretically and from a measurement perspective), research findings also showed the evolution of the concept of civic and citizenship education itself.
Addressing the deficit in chronic traumatic encephalopathy research in Africa: a call for urgent attention
Authors: Muili Abdulbasit Opeyemi , Alare Kehinde , Solagbade Oluwapelumi Samuel , Kuol Piel Panther 
Published: February 2025

Abstract
Chronic traumatic Encephalopathy (CTE) has been linked to an increase in the mortality of contact sport athletes in the USA, most especially in the early 2000s due to limited information on its existence. The lack of understanding of its existence resulted in delayed diagnosis and inadequate treatment of the disease for affected individuals.
Africa faces a similar gap as awareness and research on CTE remain limited in the region where active participation in contact sports is rising. If no drastic action is taken to mitigate the gap, the region may face similar health consequences in the future.
Various challenges responsible for the gap can be attributed to limited infrastructure, limited funding opportunities, and sociocultural factors. To address these challenges, a multifaceted approach is necessary through increasing funding, integrating CTE education into the medical curriculum, improving infrastructure, and resolving sociocultural myths about organ donation.
Revealing Sarcophyton extract's alleviating potential against gentamicin-induced renal and testicular toxicity in rat model
Authors: Badr Nada S. , Talaat Aml , El-Saidy Salwa A. , Ghoneim Aml Zaki Ahmed 
Published: February 2025

Abstract

Background
Gentamicin (GEN) is a potent antibiotic known for inducing oxidative stress and causing adverse effects in the kidneys and testes. Sarcophyton species possess significant antioxidant and anti-inflammatory properties, making them potential candidates for addressing oxidative stress and inflammation-related gentamicin toxicity. The study aims to explore the antioxidant and anti-inflammatory properties of Sarcophyton acutum methanol extract (SAME) to counteract gentamicin effects.

Methods

Sarcophyton acutum were collected and macerated with methanol, followed by phytochemical analysis of extract. Twenty-four adult male albino rats were separated into four equal groups: Control, SAME-treated (200 mg/kg/day), GEN-treated (100 mg/kg/day), and GEN + SAME-treated rats. Various parameters, including body weight, relative kidney and testes weight, differential white blood cell count, blood urea, creatinine, luteinizing hormone, testosterone, total antioxidant capacity, myeloperoxidase activity, and histopathological changes in kidney and testes tissue, were analyzed.

Results
Phytochemical analysis revealed SAME's composition, including alkaloids, saponins, phenolics, flavonoids, and tannins, with an average total antioxidant capacity of 10.503 ± 0.632 mg AAE/g extract. GEN treatment resulted in altered body and organ weights, changes in white blood cell percentages, elevated urea and creatinine levels, reduced luteinizing hormone and testosterone, decreased renal and testicular tissue total antioxidant capacity, and increased myeloperoxidase levels in both tissues. However, the administration of SAME with GEN attenuated these effects, restoring parameters closer to control levels. Histological evaluation showed that GEN treatment induced significant renal tissue damage characterized by enlarged renal corpuscles, glomerular tuft hypertrophy, tubular dilation, and necrosis, interstitial leukocyte infiltration, and tubular hyaline cast formation. Co-administration of SAME with GEN mitigated these effects, reducing renal corpuscle swelling, tubular vacuolization, and hypertrophy and preventing hyaline deposition and leukocyte infiltration. In testicular tissue, GEN injection caused seminiferous tubule atrophy, decreased spermatogenic layer thickness, and interstitial expansion and degeneration. However, SAME administration with GEN preserved normal tubular size and spermatogenic layer thickness, reduced vacuolization, and epithelial necrosis, and maintained spermatogenesis.

Conclusion

Sarcophyton acutum methanol extract demonstrates promising protective effects against gentamicin-induced renal and testicular toxicity in rats, signifying its potential as a therapeutic agent to mitigate antibiotic-induced oxidative damage in vital organs.
Combined balloon plus stent technique for treatment of intracranial aneurysm: a systematic review and meta-analysis
Authors: Habibi Mohammad Amin , Arshadi Mohammad Reza , Gharedaghi Hossein , Rashidi Farhang , Asgarzadeh Ali 
Published: February 2025

Abstract

Background
Intracranial aneurysms are weak spots in brain vessels that are at a higher risk of rupture and hemorrhage. This review investigates the safety and efficacy of the stent plus balloon technique for intracranial aneurysm management.

Methods
PubMed, Scopus, Web of Science, and Embase were systematically searched using the related key terms such as “Stent”, “Balloon”, “Intracranial”, and “Aneurysm” until July 22, 2024.

Results
Eleven studies comprising 481 aneurysms were included. The pooled angiographic outcome rate, according to Raymond–Roy occlusion classification (RROC), for RROC1 was 64% (95% CI 50–79%, p-heterogeneity < 0.001, I2 = 87%), while for the RROC2 was 18% (95% CI 14–23%, p-heterogeneity = 0.12, I2 = 1%), and the RROC3 rate was 17% (95% CI 3–30%, p-heterogeneity < 0.001, I2 = 100%). In addition, the intraprocedural complication and adverse event rate were 17% (95% CI 3% to %32, p-heterogeneity < 0.001, I2 = 97%) and 15% (95% CI 0–30%, p-heterogeneity < 0.001, I2 = 100%), respectively. Furthermore, the overall ischemic stroke occurred in % 4 (95% CI 1–8%, p-heterogeneity < 0.001, I2 = 100%), as well as 4% (95% CI 2–10% p-heterogeneity = 0.30, I2 = 30%), respectively.

Conclusion
The balloon plus stent technique is an innovative procedure; surgeons should know about and utilize in intracranial aneurysms. Our analysis observed minimal complications and mortality during the surgical procedure, which were identical to the rates observed with alternative techniques.
Hepatitis due to Coxsackie A virus in an adult: a case report
Authors: Surendra Likith , Sharma Varun , Sivakumar Milan , Lanzendorf Tara , Varkey Thomas C. , Salehpour Kaivan 
Published: February 2025

Abstract

Background
Coxsackie A16, an Enterovirus and cause of hand-foot-and-mouth disease in children, is characterized by fever and erythematous papular eruptions. Coxsackie virus-induced hepatitis has also been established in children; however, it has been rarely reported in the adult population. We present a unique case of an adult male with acute hepatitis from Coxsackie A exposure. Furthermore, this case highlights the value of obtaining a detailed history, as practitioners may overlook minute details that are key to developing an accurate care plan.

Case presentation
The patient is a 35-year-old male who presented with acute epigastric and right upper quadrant pain. His past medical history was significant for asthma, chronic back pain taking tramadol, cholecystitis status post cholecystectomy, and an ongoing workup for autoimmune hepatitis. He was found to be febrile to 100.9 °F, with leukocytosis of 12.6 × 109 WBC/L, elevated liver function tests (LFTs) including aspartate aminotransferase (AST) of 386 U/L and alanine transaminase (ALT) of 260 U/L, and elevated total bilirubin of 2.2 mg/dL. The day after presentation, AST increased to 694 U/L, and ALT increased to 594 U/L. Physical examination, abdominal imaging, and laboratory testing for classic viral causes of hepatitis were unremarkable.
The internal medicine team initially assessed the patient to have acute hepatitis that was autoimmune or drug-induced in nature. However, after more careful history taking, the patient was found to have exposure to hand-foot-and-mouth disease through his young son. The team subsequently ordered an expanded viral panel, which was positive for Enterovirus. Based on the patient’s exposure history, this was determined to be Coxsackie A. The patient was subsequently treated successfully with supportive care.

Conclusions
Coxsackie virus causing hepatitis in adults has been rarely reported. This unique case demonstrates Coxsackie A-induced hepatitis in an adult due to exposure from a sick contact. Given the patient’s history of potential autoimmune and tramadol-induced hepatocyte damage, our case suggests that Coxsackie A may be more virulent in a patient with underlying liver vulnerability. Our case also highlights the importance of obtaining a thorough history to ensure appropriate treatment and prevent delays in management.
CitGATA7 interact with histone acetyltransferase CitHAG28 to promote citric acid degradation by regulating the glutamine synthetase pathway in citrus
Authors: Lin Xiahui , Li Shaojia , Shi Yanna , Ma Yuchen , Li Yinchun , Tan Haohan , Zhang Bo , Xu Changjie , Chen Kunsong 
Published: February 2025

Abstract
Organic acid is a crucial indicator of fruit quality traits. Citric acid, the predominant organic acid in citrus fruit, directly influences its edible quality and economic value. While the transcriptional regulatory mechanisms of citric acid metabolism have been extensively studied, the understanding about the transcriptional and epigenetic co-regulation mechanisms is limited. This study characterized a transcription factor, CitGATA7, which directly binds to and activates the expression of genes associated with the glutamine synthetase pathway regulating citric acid degradation. These genes include the aconitase encoding gene CitACO3, the isocitrate dehydrogenase encoding gene CitIDH1, and the glutamine synthetase encoding gene CitGS1. Furthermore, CitGATA7 physically interacts with the histone acetyltransferase CitHAG28 to enhance histone 3 acetylation levels near the transcription start site of CitACO3, CitIDH1, and CitGS1, thereby increasing their transcription and promoting citric acid degradation. The findings demonstrate that the CitGATA7-CitHAG28 protein complex transcriptionally regulate the expression of the GS pathway genes, i.e., CitACO3, CitIDH1, and CitGS1, via histone acetylation, thus promoting citric acid catabolism. This study establishes a direct link between transcriptional regulation and histone acetylation regarding citric acid metabolism, providing insights for strategies to manipulate organic acid accumulation in fruit.
Enhancing undrained shear strength prediction: a robust hybrid machine learning approach with naïve Bayes modeling
Authors: Fang Chen , Li Ying , Shi Yang 
Published: February 2025

Abstract
In geotechnical engineering, it is crucial to make sure that the undrained shear strength (USS) of soft, sensitive clays is accurately assessed. The accuracy in forecasting USS is pivotal for ensuring the structural integrity and stability of foundations and earthworks. Addressing this concern, advanced data-driven NB techniques are utilized to disclose the complex interactions of USS with basic soil parameters. This paper presents a novel methodology for the USS prediction in soft clays using machine learning techniques, and particularly it highlights the attention on the following five important input variables: pre-consolidation stress (PS), vertical effective stress (VES), liquid limit (LL), plastic limit (PL), and natural water content (W). These are selected in view of their well-understood impact on the USS. This study reports an innovative effort to use SHO and AOSM for the model's hyperparameter tuning, reducing heuristic methods and computationally expensive brute-force searches. This will provide a neat methodology for improving accuracy in USS predictions and maintaining the optimality of the model performance. The results, therefore, provide geotechnical engineers and researchers with considerable benefits. They give a sound basis that is data-driven for the assessment of USS in soft sensitive clays and advance the safety and stability of civil engineering projects.
Brainstem Noradrenergic Neuronal Populations: Dual Effects on Regulating GnRH and LH Secretion
Authors: Carrasco Rodrigo A , Breen Kellie M 
Published: February 2025

Abstract
Noradrenergic neurons are a brain network that integrate viscero-sensorial signals to modulate neural and neuroendocrine function. Although it has been known for decades that noradrenergic neural circuits influence neuroendocrine and reproductive function, the cellular and molecular players involved remain largely unknown. The objective of this review is to summarize past and current knowledge regarding the influence of brainstem noradrenergic systems on GnRH and gonadotrophin secretion. The main noradrenergic cell groups A1, A2, and A6, known as the ventrolateral medulla, nucleus of the solitary tract, and locus coeruleus, respectively, are involved in the control of reproductive neuroendocrine secretion. Current evidence suggests that brainstem noradrenergic circuits promote the generation and maintenance of the LH surge in both spontaneous (rats, sheep) and induced (rabbit, ferret) ovulators. In contrast, recent studies have established that LH pulsatile secretion is suppressed by specific activation of brainstem noradrenergic cell groups. The duality of the GnRH/LH response to noradrenaline reflects the inherent complexity of hindbrain noradrenaline neurons, which are responsive to stressors and gonadal steroids (ie, estradiol) and coexpress a variety of neurotransmitters and neuropeptides. Therefore, elucidating the organization and functionality of brainstem noradrenergic systems will provide targets for controlling reproduction and understanding the interconnection with stress.
Euglycemic Ketoacidosis Following Coadministration of an SGLT2 Inhibitor and Tirzepatide
Authors: Louwagie Eli J , Diego Jessica N , Farooqi Crystal S , Kamal Madiha M 
Published: February 2025

Abstract
Euglycemic ketoacidosis (EKA) is a life-threatening condition characterized by ketone production leading to systemic acidosis, dehydration, and end-organ damage. It presents similarly to diabetic ketoacidosis, except that patients have normal to slightly elevated blood glucose levels. EKA is an increasingly recognized complication of sodium-glucose cotransporter 2 (SGLT2) inhibitors and glucagon-like peptide-1 (GLP-1) receptor agonists. Recently the novel dual GLP-1 and glucose-dependent insulinotropic polypeptide receptor agonist tirzepatide was approved for treatment of diabetes and weight loss. Here, we describe a unique case in which a patient placed on both an SGLT2 inhibitor and tirzepatide to treat type 2 diabetes was admitted to the intensive care unit (ICU) for EKA. To our knowledge, this is the first case detailing a patient developing this serious condition after starting tirzepatide for diabetes. The patient required treatment and monitoring in an ICU to make a full recovery. As tirzepatide is a relatively new medication whose side effect profile has yet to be fully characterized, clinicians should be aware of this rare yet potentially fatal complication.