bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advanced glycation end products measured by skin autofluorescence and subclinical cardiovascular disease: the Rotterdam Study
Authors: Chen Jinluan , Arshi Banafsheh , Waqas Komal , Lu Tianqi , Bos Daniel , Ikram M. Arfan , Uitterlinden André G. , Kavousi Maryam , Zillikens M. Carola 
Published: November 2023

Abstract
Background
Advanced glycation end products (AGEs) have been linked to cardiovascular disease (CVD), especially coronary heart disease (CHD), but their role in CVD pathogenesis remains unclear. Therefore, we investigated cross-sectional associations of skin AGEs with subclinical atherosclerosis, arterial stiffness, and hypertension after confirming their relation with CHD.

Methods
In the population-based Rotterdam Study, skin AGEs were measured as skin autofluorescence (SAF). Prevalent MI was obtained from digital medical records. Carotid plaques, carotid intima-media thickness (IMT), coronary artery calcification (CAC), pulse wave velocity (PWV), and hypertension were assessed. Associations of SAF with endophenotypes were investigated in logistic and linear regression models adjusting for common cardiovascular risk factors. Effect modification by sex, diabetes mellitus, and chronic kidney disease (CKD) was tested.

Results
3001 participants were included (mean age 73 (SD 9) years, 57% women). One unit higher SAF was associated with the presence of carotid plaques (OR 1.2 (0.92, 1.57)), a higher max IMT (0.08 SD (0.01, 0.15)), higher CAC (OR 2.2 (1.39, 3.48)), and PWV (0.09 SD (0.01, 0.16)), but not with hypertension (OR 0.99 (0.81, 1.21)). The associations with endophenotypes were more pronounced in men and participants with diabetes or CKD with significant interactions.

Conclusions
Previously documented associations between SAF and CVD, also found in our study, may be explained by the endophenotypes atherosclerosis and arterial stiffness, especially in men and individuals with diabetes or CKD, but not by hypertension. Longitudinal studies are needed to replicate these findings and to test if SAF is an independent risk factor or biomarker of CVD.
Trial registration: The Rotterdam Study has been entered into the Netherlands National Trial Register (NTR; www.trialregister.nl) and the WHO International Clinical Trials Registry Platform (ICTRP; www.who.int/ictrp/network/primary/en/) under shared catalogue number NTR6831.
Regulating bulb dormancy release and flowering in lily through chemical modulation of intercellular communication
Authors: Zhao Yajie , Pan Wenqiang , Xin Yin , Wu Jingxiang , Li Rong , Shi Jinxin , Long Shuo , Qu Lianwei , Yang Yingdong , Yi Mingfang , Wu Jian 
Published: November 2023

AbstractLily is a bulbous plant with an endogenous dormancy trait. Fine-tuning bulb dormancy release is still a challenge in the development of bulb storage technology. In this study, we identified three regulators of symplastic transport, 2,3-Butanedione oxime (BDM), N-Ethyl maleimide (NEM), and 2-Deoxy-D-glucose (DDG), that also regulate bulb dormancy release. We demonstrated that BDM and DDG inhibited callose synthesis between cells and promoted symplastic transport and soluble sugars in the shoot apical meristem (SAM), eventually accelerating bulb dormancy release and flowering in lilies. Conversely, NEM had the opposite effect. These three regulators can be flexibly applied to either accelerate or delay lily bulb dormancy release.
Positive single-center randomized trials and subsequent multicenter randomized trials in critically ill patients: a systematic review
Authors: Kotani Yuki , Turi Stefano , Ortalda Alessandro , Baiardo Redaelli Martina , Marchetti Cristiano , Landoni Giovanni , Bellomo Rinaldo 
Published: November 2023

Abstract
Background
It is unclear how often survival benefits observed in single-center randomized controlled trials (sRCTs) involving critically ill patients are confirmed by subsequent multicenter randomized controlled trials (mRCTs). We aimed to perform a systemic literature review of sRCTs with a statistically significant mortality reduction and to evaluate whether subsequent mRCTs confirmed such reduction.

Methods
We searched PubMed for sRCTs published in the New England Journal of Medicine, JAMA, or Lancet, from inception until December 31, 2016. We selected studies reporting a statistically significant mortality decrease using any intervention (drug, technique, or strategy) in adult critically ill patients. We then searched for subsequent mRCTs addressing the same research question tested by the sRCT. We compared the concordance of results between sRCTs and mRCTs when any mRCT was available. We registered this systematic review in the PROSPERO International Prospective Register of Systematic Reviews (CRD42023455362).

Results
We identified 19 sRCTs reporting a significant mortality reduction in adult critically ill patients. For 16 sRCTs, we identified at least one subsequent mRCT (24 trials in total), while the interventions from three sRCTs have not yet been addressed in a subsequent mRCT. Only one out of 16 sRCTs (6%) was followed by a mRCT replicating a significant mortality reduction; 14 (88%) were followed by mRCTs with no mortality difference. The positive finding of one sRCT (6%) on intensive glycemic control was contradicted by a subsequent mRCT showing a significant mortality increase. Of the 14 sRCTs referenced at least once in international guidelines, six (43%) have since been either removed or suggested against in the most recent versions of relevant guidelines.

Conclusion
Mortality reduction shown by sRCTs is typically not replicated by mRCTs. The findings of sRCTs should be considered hypothesis-generating and should not contribute to guidelines.
Long-term decitabine/retinoic acid maintenance treatment in an elderly sAML patient with high-risk genetics
Authors: Javorniczky Nora Rebeka , Grishina Olga , Hund Inga , Pantic Milena , Pfeifer Dietmar , Schmoor Claudia , Thomas Johanna , Duyster Justus , Becker Heiko , Lübbert Michael 
Published: November 2023

AbstractElderly patients with AML ineligible for induction have a dismal prognosis; hence disease stabilization is a primary treatment goal. This case of a 75-year-old patient with secondary AML receiving the combination of decitabine and ATRA (within the DECIDER trial, NCT00867672) demonstrates an above-average survival. The therapy administered over 52 cycles led to complete molecular and hematological remission and resulted in 5.3 years overall survival. Clonal evolution of the leukemic clone could be demonstrated using DNA sequencing methods. According to the literature, this case constitutes the longest continued HMA exposure in an elderly AML patient ineligible for standard chemotherapy.
Oxidative stress regulation and related metabolic pathways in epithelial–mesenchymal transition of breast cancer stem cells
Authors: Farahzadi Raheleh , Valipour Behnaz , Fathi Ezzatollah , Pirmoradi Samaneh , Molavi Ommoleila , Montazersaheb Soheila , Sanaat Zohreh 
Published: November 2023

AbstractEpithelial–mesenchymal transition (EMT) is a cell remodeling process in which epithelial cells undergo a reversible phenotype switch via the loss of adhesion capacity and acquisition of mesenchymal characteristics. In other words, EMT activation can increase invasiveness and metastatic properties, and prevent the sensitivity of tumor cells to chemotherapeutics, as mesenchymal cells have a higher resistance to chemotherapy and immunotherapy. EMT is orchestrated by a complex and multifactorial network, often linked to episodic, transient, or partial events. A variety of factors have been implicated in EMT development. Based on this concept, multiple metabolic pathways and master transcription factors, such as Snail, Twist, and ZEB, can drive the EMT. Emerging evidence suggests that oxidative stress plays a significant role in EMT induction. One emerging theory is that reducing mitochondrial-derived reactive oxygen species production may contribute to EMT development. This review describes how metabolic pathways and transcription factors are linked to EMT induction and addresses the involvement of signaling pathways.
Therapeutic uses of oxytocin in stress-related neuropsychiatric disorders
Authors: Zhang Sen , Zhang Ying-Dan , Shi Dong-Dong , Wang Zhen 
Published: November 2023

AbstractOxytocin (OXT), produced and secreted in the paraventricular nucleus and supraoptic nucleus of magnocellular and parvocellular neurons. The diverse presence and activity of oxytocin suggests a potential for this neuropeptide in the pathogenesis and treatment of stress-related neuropsychiatric disorders (anxiety, depression and post-traumatic stress disorder (PTSD)). For a more comprehensive understanding of the mechanism of OXT's anti-stress action, the signaling cascade of OXT binding to targeting stress were summarized. Then the advance of OXT treatment in depression, anxiety, PTSD and the major projection region of OXT neuron were discussed. Further, the efficacy of endogenous and exogenous OXT in stress responses were highlighted in this review. To augment the level of OXT in stress-related neuropsychiatric disorders, current biological strategies were summarized to shed a light on the treatment of stress-induced psychiatric disorders. We also conclude some of the major puzzles in the therapeutic uses of OXT in stress-related neuropsychiatric disorders. Although some questions remain to be resolved, OXT has an enormous potential therapeutic use as a hormone that regulates stress responses.
Networks in Japanese regional agro-food economies: an empirical exploration of the network linkage model
Authors: Ishikawa Kiyotaka 
Published: November 2023

AbstractNetwork linkage is important in evaluating macroeconomic performance since input‒output networks across industries are asymmetric and respond differently to external shocks. While most studies implicitly assume elastic substitution between intermediates and factors using Cobb‒Douglas models, this is often improbable since the input‒output structure may change due to the shocks, which would be observed as nonlinearities in macroeconomic impacts on sectoral shocks. Additionally, considering regionally located sectors such as the agriculture and food-processing industries, the propagation of sectoral shocks can be interregionally correlated. This study employs the network linkage model to empirically verify the interaction of agro-food sectoral shocks in regional outcomes. By comparing the network effects influencing the national economy and regional economy, the superiority of considering intraregional networks among agro-food sectors is empirically verified; thus, productivity shocks arising in these industries propagate more intensively within their own region.
The CAR macrophage cells, a novel generation of chimeric antigen-based approach against solid tumors
Authors: Hadiloo Kaveh , Taremi Siavash , Heidari Mahmood , Esmaeilzadeh Abdolreza 
Published: November 2023

AbstractToday, adoptive cell therapy has many successes in cancer therapy, and this subject is brilliant in using chimeric antigen receptor T cells. The CAR T cell therapy, with its FDA-approved drugs, could treat several types of hematological malignancies and thus be very attractive for treating solid cancer. Unfortunately, the CAR T cell cannot be very functional in solid cancers due to its unique features. This treatment method has several harmful adverse effects that limit their applications, so novel treatments must use new cells like NK cells, NKT cells, and macrophage cells. Among these cells, the CAR macrophage cells, due to their brilliant innate features, are more attractive for solid tumor therapy and seem to be a better candidate for the prior treatment methods. The CAR macrophage cells have vital roles in the tumor microenvironment and, with their direct effect, can eliminate tumor cells efficiently. In addition, the CAR macrophage cells, due to being a part of the innate immune system, attended the tumor sites. With the high infiltration, their therapy modulations are more effective. This review investigates the last achievements in CAR-macrophage cells and the future of this immunotherapy treatment method.
From immunology to artificial intelligence: revolutionizing latent tuberculosis infection diagnosis with machine learning
Authors: Li Lin-Sheng , Yang Ling , Zhuang Li , Ye Zhao-Yang , Zhao Wei-Guo , Gong Wen-Ping 
Published: November 2023

AbstractLatent tuberculosis infection (LTBI) has become a major source of active tuberculosis (ATB). Although the tuberculin skin test and interferon-gamma release assay can be used to diagnose LTBI, these methods can only differentiate infected individuals from healthy ones but cannot discriminate between LTBI and ATB. Thus, the diagnosis of LTBI faces many challenges, such as the lack of effective biomarkers from Mycobacterium tuberculosis (MTB) for distinguishing LTBI, the low diagnostic efficacy of biomarkers derived from the human host, and the absence of a gold standard to differentiate between LTBI and ATB. Sputum culture, as the gold standard for diagnosing tuberculosis, is time-consuming and cannot distinguish between ATB and LTBI. In this article, we review the pathogenesis of MTB and the immune mechanisms of the host in LTBI, including the innate and adaptive immune responses, multiple immune evasion mechanisms of MTB, and epigenetic regulation. Based on this knowledge, we summarize the current status and challenges in diagnosing LTBI and present the application of machine learning (ML) in LTBI diagnosis, as well as the advantages and limitations of ML in this context. Finally, we discuss the future development directions of ML applied to LTBI diagnosis.
Recovery from indomethacin-induced gastrointestinal bleeding by treatment with teprenone
Authors: Deguchi Saori , Iwakami Ayusa , Tujigiwa Mizuki , Otake Hiroko , Mano Yu , Yamamoto Naoki , Nakazawa Yosuke , Misra Manju , Nagai Noriaki 
Published: November 2023

Abstract
Background
Gastrointestinal injuries caused by nonsteroidal anti-inflammatory drugs (NSAIDs) is a serious side effect in patients with rheumatoid arthritis (RA). However, effective therapeutic strategies have yet to be established. In this study, we investigated the therapeutic effects of teprenone (TEP), a gastric mucosal protective drug, on NSAID-induced gastrointestinal injuries in rats with RA (AA rats).

Methods
Gastrointestinal injury was induced by oral administration of indomethacin (IMC), a typical NSAID. TEP was orally administered after IMC-induced gastrointestinal bleeding, and the stomach, jejunum, and ileum were excised.

Results
On day 14 of IMC administration, lesion areas in the stomach, jejunum, and ileum were significantly larger in AA rats than in normal rats. When TEP was orally administered to AA rats, the lesion areas in the stomach, jejunum, and ileum significantly decreased compared with those in control rats (IMC-induced AA rats). Therefore, we measured NOS2 mRNA and NO levels, which were significantly decreased in rats with IMC-induced AA after treatment with TEP.

Conclusions
These results suggest that the oral administration of TEP may be useful for the treatment of NSAID-induced gastrointestinal injuries in patients with RA.
Study protocol: Novel Methods for Implementing Measurement-Based Care with youth in Low-Resource Environments (NIMBLE)
Authors: Martinez Ruben G. , Weiner Bryan J. , Meza Rosemary D. , Dorsey Shannon , Palazzo Lorella G. , Matson Abigail , Bain Carolyn , Mettert Kayne D. , Pullmann Michael D. , Lewis Cara C. 
Published: November 2023

Abstract
Background
For youth receiving care in community mental health centers, comorbidities are the rule rather than the exception. Using measurement-based care (MBC), or the routine evaluation of symptoms to inform care decisions, as the foundation of treatment for youth with comorbid problems significantly improves the impact of psychotherapy by focusing care and building engagement and alliance. MBC increases the rate of symptom improvement, detects clients who would otherwise deteriorate, and alerts clinicians to non-responders. Despite its demonstrated utility, MBC is rarely implemented with fidelity; less than 15% of providers report using MBC per recommendations. Previous efforts to support MBC implementation have yielded suboptimal outcomes, in part, due to organizations’ challenges with identifying and prioritizing barriers and selecting and developing strategies to overcome them. New methods are needed for identifying and prioritizing barriers, and matching strategies to barriers to optimize MBC implementation and treatment quality to improve youth mental health outcomes in community settings.

Methods
Pragmatic implementation methods will be piloted in four diverse community mental health centers. Methods include (a) rapid evidence synthesis; (b) rapid ethnography; (c) design kits (e.g., kits with disposable cameras, journals, maps); (d) barrier prioritization, and (e) causal pathway diagramming. These activities will generate actionable barriers; subsequently, we will use facilitated group processes to prioritize barriers and develop causal pathway diagrams to match strategies to barriers to create implementation plans that optimize MBC fidelity (Aim 1). We will track strategy deployment for 6 months, then compare MBC fidelity for another 6 months post-implementation with data from 2 years of historical controls (Aim 2). Finally, we will co-design a toolkit for design kit methods with youth and the practice and scientific communities (Aim 3).

Discussion
Optimizing MBC implementation in community mental health centers could transform youth mental health care by ensuring the most pressing symptoms are targeted early in treatment. The discussion section highlights expected challenges and limits to using the five methods, including recruitment and engagement given the high pressure on community mental health settings.

Trial registration
Clinicaltrials.gov. NCT05644756. Registered on 18 November 2022. This trial was retrospectively registered.
SRAP and IRAP revealed molecular characterization and genetic relationships among cowpea (Vigna unguiculata L.) irradiated by gamma-ray
Authors: Azzam Clara R. , Sultan Fadia M. , Rizk Mokhtar S. , Ahmed Mohamed Z. S. , Ibrahim Shafik D. , Noureldeen Ahmed , Ali Esmat F. , Darwish Hadeer , Khaled Khaled Adly M. 
Published: November 2023

Abstract
Background
Cowpea is a high-protein legume that can be grown in many environments. Gamma radiation can modify plant metabolism and growth. An experiment was conducted to determine the effect of different gamma-ray doses on cowpea yield and growth. The results showed that gamma radiation had a significant effect on cowpea yield and growth, with the highest yields and growth rates observed at lower doses. Higher doses had a negative effect on cowpea yield and growth. The study suggests that gamma radiation can be used to improve cowpea yield and growth, but that the optimal dose must be determined for each specific cultivar and environment.

Results
Results specified that gamma ray significantly affected growth characters and forage yield. Gamma-ray dose of 50 Gy in M1 and M2 generations gave the highest values for all studied characteristics compared to the control and all other irradiation doses, except the crude fiber, followed by 75 and 100 Gy doses for all characters, except number of tillers plant−1 and crude fiber. Seven combinations of SRAP produced 227 loci with an average polymorphism percentage of 85%. The allele frequency of target loci ranged between 0.29 and 0.60, and the PIC was 0.41–0.50 in range, while the GTS% of SRAP combinations ranged from 36 to 63%. The genetic similarities ranged between − 4 and 30%. Ten primers produced 450 loci with a polymorphism percentage of 88%. The allele frequency of target loci ranged between 0.28 and 0.46. The PIC was 0.40–0.50 in range, and the GTS% of IRAP primers ranged from 29 to 71%. The genetic similarities ranged from 8 to 37%.

Conclusions
SRAP and IRAP analysis revealed more than two clusters of treated plants. In silico analysis showed that some SRAP primers could align with genes in cowpea and related genomes. The SRAP-F12R9 primer is unique to Phaseolus vulgaris, while the SRAP-F13R15 and SRAP-F13em1 primers align with genes on different chromosomes in cowpea cultivar Xiabao 2.
Internal carotid bulb occlusion by a giant thrombus after thoracoscopic left upper lung lobectomy successfully treated with endovascular stenting: a case report
Authors: Saito Tomohito , Kunieda Takenobu , Hashimoto Yasumasa , Ishida Mitsuaki , Maru Natsumi , Utsumi Takahiro , Matsui Hiroshi , Taniguchi Yohei , Hino Haruaki , Murakawa Tomohiro 
Published: November 2023

Abstract
Background
Perioperative acute ischemic stroke following lung resection is relatively rare, though a devastating complication. Specifically, patients undergoing left upper lung lobectomy for lung cancer are likely to develop perioperative acute ischemic stroke.

Case presentation
A 67-year-old man underwent thoracoscopic left upper lung lobectomy for lung adenocarcinoma; he experienced sudden-onset left hemiparesis and dysarthria on the morning of the second postoperative day. Angiography revealed occlusion of the bulbs of the right internal and external carotid arteries by a giant thrombus, which could not be removed through endovascular thrombectomy. We deployed a stent at the right carotid bifurcation to foist the giant thrombus, achieving revascularization 4 h after the onset. Treatment response was assessed as good improvement with a modified Rankin scale score of 0, and the patient was discharged home 19 days after symptom onset.

Conclusions
We present a unique case of carotid bulb thromboembolism resulting from a giant thrombus following thoracoscopic left upper lung lobectomy, for which endovascular stenting was effective.
Therapy of parapneumonic empyema in children: a protocol for a scoping review of the literature
Authors: Buonsenso Danilo , Cusenza Francesca , Passadore Lucrezia , Bonanno Francesca , Calanca Carolina , Rasmi Sonia , Mariani Francesco , Esposito Susanna Maria Roberta 
Published: November 2023

Background: Empyema (the presence of pus in the pleural space) is a severe complication of community-acquired pneumonia and significant cause of morbidity, but, fortunately, not mortality in children. Between 0.6 and 2% of pneumonias are complicated by empyema and the three main pathogens involved are Streptococcus pneumoniae, Staphylococcus aureus and group A Streptococcus 1,2,3,4. Optimal management in children, especially the choice of antibiotics, method of administration and duration of therapy, are still matter of debate and currently, lack of strong specific recommendations. This paper displays the study protocol for a scoping review that aims to summarize the available literature on the microbiological epidemiology, the treatment options, and the outcomes of pleural empyema in pediatric population. Methods: Comprehensive research combining the terms pediatric (children aged 0 to 18 years) and pleural empyema will be performed on PubMed and SCOPUS to identify all eligible studies. At first, two reviewers will screen their abstract and then their full text to detecting the articles that meet the inclusion criteria. This work will be carried out independently, everyone on a different Excel spreadsheet and each researcher will be blinded to the decision of the other researcher. When the process will be completed, in case of discordance, any disagreement will be identified and resolved through discussion or with the help, when needed, of a third author. Dissemination: The findings of this review will be published in a peer-reviewed journal.