bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biosorption of Antimony Species by Red Macroalgae and Cyanobacteria
Authors: Silva Renata Martins da , Viana Adriano Gonçalves , Anjos Vanessa Egéa dos 
Published: October 2023

Abstract

A biotechnological study with the Porphyra tenera (red macroalgae, “Nori”) and Spirulina platensis (cyanobacteria) was performed to evaluate the feasibility of non-living biomass as biosorbent for inorganic antimony species to be used in the remediation technology or as solid phase for analytical purposes such as preconcentration. The biosorption of Sb(III) and Sb(V) was studied using batch technique under similar conditions of the aquatic environments. High values of biosorption (over 70%) of both Sb species were determined and factors such as dosage of the biomass, contact time and pH, practically did not influenced in the sorption. The desorption was evaluated using different concentrations of HCl and HNO3. The biosorbents were characterized by X-ray diffraction, scanning electron microscopy (SEM), infrared spectroscopy (FTIR), surface area (BET) and zeta potential. The FTIR analysis after biosorption of the Sb species suggested the involvement of protonated functional groups as amino, carboxyl, and hydroxyl in the interaction mechanism. Thus, both biosorbents are suitable biosorption of Sb from aqueous solution.
Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)
Authors: Xi Peng , Xu Xinzhu , Wang Wenyi , Qiao Liang , Fu Yunzhe , Ge Xichuan , Zhao Kun , Zhanghao Karl , Guan Meiling , Chen Xin , Li Meiqi , Jin Dayong 
Published: October 2023

Abstract
Parallel acquisition-readout SIM (PAR-SIM) achieves a fundamentally stupendous information temporal flux of 132.9 Mpixels per second, larger than 9.6-fold of the latest techniques, with the lowest SNR of -3.42 dB and 100 nm resolution. It successfully reconstructs diverse cellular organelles in dual wavelengths. Notably, mitochondrial dynamic tubulation and ongoing membrane fusion processes in live COS-7 cell have been captured with PAR-SIM at 408 Hz, without degrading the image reconstruction quality.
Gas exchange and chlorophyll fluorescence responses of Camellia sinensis grown under various culivations in different seasons
Authors: Chen Chung-I , Lin Kuan-Hung , Huang Meng-Yuan , Yang Chih-Kai , Lin Yu-Hsiu , Hsueh Mei-Li , Lee Li-Hua , Lin Shiou-Ruei , Wang Ching-Wen 
Published: October 2023

Abstract

Sod culture (SC) and conventional agriculture (CA) represent two distinct field management approaches utilized in the cultivation of tea plants in Taiwan. In this study, we employed gas exchange and chlorophyll fluorescence techniques to assess the impact of SC and CA methods on the photosynthetic machinery of Camellia sinensis cv. TTES No.12 (Jhinhsuan) in response to variable light intensities across different seasons. In spring, at photosynthetic photon flux densities (PPFD) ranging from 800 to 2,000 μmol photon m−2 s−1, the net photosynthesis rate (Pn), stomatal conductance (Gs), electron transport rate (ETR), and ΔF/Fm' and Fv/Fm values for plants grown using SC were comparatively higher than those cultivated under CA. Conversely, the non-photochemical quenching (NPQ) values for SC-grown plants were relatively lower compared to those grown under CA at 800 to 2,000 PPFD in spring. Additionally, when tea plants were exposed to PPFD levels below 1,500 μmol photon m−2 s−1, there was a concurrent increase in Pn, Gs, ETR, and NPQ. These photosynthetic parameters are crucial for devising models that optimize cultivation practices across varying seasons and specific tillage requirements, and for predicting photosynthetic and respiratory responses of tea plants to seasonally or artificially altered light irradiances. The observed positive impacts of SC on maximum photosynthetic rate (Amax), Fv/Fm, Gs, water-use efficiency (WUE), and ETR suggest that SC is advantageous for enhancing the productivity and stress resilience of tea plants, thereby offering a novel management model for tea gardens.
Effect of music on hemodynamic fluctuations in women during induction of general anesthesia: a prospective randomized controlled multicenter trial
Authors: Wang Jie , Jiang Linghui , Chen Wannan , Wang Zhiyao , Liu Manman , Tong Jie , Ding Ming , Zhong Jing , Miao Changhong , xiong wanxia 
Published: October 2023

Abstract

Background
Anxiety can cause perioperative stress and increase sympathetic nervous system activity, potentially contributing to negative clinical outcomes, including hemodynamic instability, prolonged anesthesia induction and cardiac arrhythmias. We aim to investigate the effect of music on hemodynamic fluctuations during induction of general anesthesia and reducing preoperative anxiety for women underwent elective non-cardiac surgery.
Methods
It is a multicenter, double-blind, randomized, parallel-group clinical trial. Participants were women who were underwent elective non-cardiac surgery under general anesthesia. Patients were randomized 1:1 to either a music intervention group (MI) or a control group (Control). The MI participants listened to their preferred music for more than 30 minutes in the waiting area. The State-Trait Anxiety Inventory (STAI) was used to measure anxiety levels in the groups, and hemodynamic parameters (heart rate [HR], mean arterial pressure [MAP]) were continuously recorded before induction (T0), at loss of consciousness (T1), immediately before intubation (T2), and after intubation (T3). Intubation-related adverse events were also recorded. The primary outcome was the incidence of MAP changes more than 20% above baseline during T0–T2.
Results
A total of 164 patients were included in the final analyses. Incidence of MAP instability (change greater than 20% above baseline) during T0–T2 was lower in the MI (P = 0.03), and the 95% confidence interval for the rate difference (− 0.2708 to − 0.0164) demonstrated the superiority of MI. HR instability were less frequent in MI participants both in T0–T2 (P = 0.04) and T2–T3 (P = 0.005). The overall incidence of preoperative anxiety was 53.7% (88/164). After the music intervention, the mean score of STAI was significantly lower in the MI than in the Control, with a between-group difference of 8.01 (P < 0.001) by analysis of covariance.
Conclusions
Preoperative music intervention effectively prevented hemodynamic instability during anesthesia induction and significantly reduced preoperative anxiety in women underwent elective non-cardiac surgery.
Trial Registration:
The study is registered in the Chinese Clinical Trial Registry on 26/11/2020 (ChiCTR2000040254, http://www.chictr.org.cn/showprojen.aspx?proj=64383).
Effect of different defect areas of medial femoral condyle cartilage on Meniscal articular Cartilage stress: three-dimensional finite element analysis
Authors: Yeming Zhang , Ling Zhang , Hongxing Gao , Di Wu , Chaokai Zhang , ShaoRong Liu , Yuanzuo Zhang , Song min , *Qing *Li 
Published: October 2023

Abstract
Background
The present-day three-dimensional finite element analysis (FEM) is widely used in human biomechanics research, but there are few studies on the effect of cartilage defect of medial femoral condyle on meniscus stress of knee in full extension.
OBJECTIVE
The purpose of this study was to explore the changing trend of meniscus stress during the changing process of femoral medial condyle cartilage defect,to provide the simulated biomechanical insights into femoral condyle cartilage defect on meniscus,in order to provide the theory reference value for femoral condyle cartilage repair and clinical prevention of meniscus injury.)
Methods
One healthy male volunteer was chosen to obtain the three-dimensional digital finite element model. The material mechanical properties were input into the model with divided grids in the Abaques software. Using the boundary conditions and the loads to the model, the structural nonlinear finite element was calculated. Firstly, the stress distribution of the normal knee cartilage meniscus was observed under the condition of load, and then the stress distribution of the meniscus was observed under the condition of load when the medial femoral condyle cartilage was in different defects (0, 6, 8, 10, 12, 14, 16, 18 and 20 mm). The stress changing on the meniscus was analyzed when the defect of the femoral condyle cartilage happened.
Results
When the diameter of femoral medial condyle cartilage defect was no less than 10 mm, the stress distribution of the meniscus was statistically different (the value of P is less than 0.05). When the medial femoral condyle cartilage defect occurred in different areas(d), the stress peak was mainly concentrated in the body part and posterior corner of the meniscus. Moreover, we found that the maximum, minimum and average stress of meniscus increased with the increased defect area. We also found that the increased defect area can lead to the increases of stress of the meniscus.
Dietary Variation Effect on Life History Traits and Energy Storage in Neotropical Species of Drosophila (Diptera; Drosophilidae)
Authors: Santos Camila Heloise dos , Gustani Emanuele Cristina , Machado Luciana Paes de Barros , Mateus Rogerio Pincela 
Published: October 2023

Abstract

The ability of an organism to respond to nutritional stress can be a plastic character under the action of natural selection, affecting several characteristics, including life history and energy storage. The Drosophila genus (Diptera; Drosophilidae) presents high variability regarding natural resource exploration. However, most works on this theme have studied the model species D. melanogaster (J.W.Meigen) and little is known about Neotropical species. Thus, the current work evaluated the effects of three diets, with different carbohydrate:protein ratios, on life history (viability and development time) and metabolic pools (triglycerides, glycogen, and total soluble protein contents) of three Neotropical species. Our results showed that Drosophila willistoni (A.H.Sturtevant) (Sophophora subgenus) was viable on all diets, while D. maculifrons (O.Duda) (Drosophila subgenus) was not, and D. ornatifrons (O.Duda) (Drosophila subgenus) was barely viable on the sugary diet. This diet increased the development time of D. willistoni and D. ornatifrons, and D. willistoni glycogen content. Thus, the viability of D. maculifrons and D. ornatifrons seems to depend on a certain amount of protein and/or a low concentration of carbohydrate in the diet. A more evident effect of the diets on triglyceride and protein pools was detected in D. ornatifrons, which could be related to its capacity to explore dung and carrion as food resource in nature. The results found demonstrated that the evolutionary history and differential adaptations to natural macronutrient resources are important to define the amplitude of response that a species can present when faced with dietary variation.
The effect of COVID-19 on TB case detection and treatment outcomes in Ethiopia, Addis Ababa city.
Authors: Kebede Dawit , Demissie Meaza , Taye Hawult , Baye Netsanet , Genet Simon 
Published: October 2023

Abstract

Background:
In the context of the global tuberculosis (TB) epidemic, the COVID-19 pandemic threatens to reverse promising progress toward global TB targets. During the pandemic, similar to most countries, the Ethiopian government announced movement restrictions targeting the control of the spread of COVID-19.
Methods:
A quantitative retrospective study was used based on secondary data collected from the Addis Ababa city-Health Bureau DHIS-2 database for a study period of four years (April/2018-March/2021). Two-year pre- and two intra-COVID-19 periods. The study area included all health facilities providing TB care in Addis Ababa. The data were collected using a structured format, and analysis was performed using STAT version 17 and Excel software.
Result.
Overall, 13190 TB cases were detected during the COVID-19 era and 15038 in pre-COVID-19, representing a 12.3% decline with P= 0.0092. The highest decrease was in Q-1 and Q-2 by (30% and 29.8%) CI 95% during COVID-19 in the first year and in the second year in Q-4 by 21.2%.
All forms of TB case notifications were significantly decreased (P< 0.001). RELAPSE and clinically diagnosed TB showed the highest decrease (30%, P<0.001 and 20.6%, P<0.001) CI 95%.
Of TB treatment outcomes, completed, lost to follow-up and death rates had better outcome rates during the COVID-19 era. In the first year after the COVID-19 pandemic, the TB treatment completion rate was higher (63.8% vs. 60%, P=0.0001) (CI 95%), with a lower rate of loss to follow-up (1.8% vs 1.9%, P=0.659) and death rate (3.8% vs 4.8% P=0.0034). In the second year after the COVID-19 pandemic, the TB treatment completion rate was higher (64.5% vs. 62.4%, P= 0.0003), with a lower rate of loss to follow-up (1.7% vs 1.9%, P=0.2036) and death rate (4.3% vs 4.6%, P=0.2232).
Conclusion
Finally, the unpredicted study finding of treatment outcomes had the potential to change the existing DOT treatment policy in Ethiopia. In view of this, further study in a broader context at the health minister level is recommended. Moreover, to mitigate the catastrophic impact of the COVID-19 pandemic on TB case detection, improving access to quality TB care services is mandatory to enhance TB detection. Ultimately, it is able to reverse the catastrophic impact of the COVID-19 pandemic on TB care program activities in Addis Ababa, Ethiopia.
The effect of hydraulic and leaf photosynthesis properties on the spread and distribution of Dasiphora fruticosa in Qinghai-Tibet Plateau alpine meadows
Authors: Fan Baoli , Ding Nana , Gao Pengfei , Tian Tingting , Ai Dongxia , Wan Yongkuan , Sun Kun 
Published: October 2023

Abstract

Background and Aims Qinghai-Tibet Plateau is the largest alpine grassland area in the world. Alpine region is a typical and unique plateau ecosystem. Natural and human-induced factors have resulted in shrub encroachment in the Qinghai-Tibet Plateau alpine meadows. Yet, the role of functional traits of different plant organs in shrub encroachment remain insufficiently explored. Methods Here, we measured stem and leaf hydraulics, leaf photosynthesis characteristics, and other functional traits ofD. fruticosain different habitats. Results We found that hydraulic traits (KL, KS, π0, ε) and leaf photosynthetic capacity (Aa, Am, gs) were key factors in the shrub encroachment ofD. fruticosa. In addition, variations in root average diameter (RAD) and specific root length (SRL) were mainly influenced by soil total nitrogen (STN) and soil total phosphorus (STP) between two habitats. On soil water and nutrient deficient sunny slopes, below-ground functional traits ofD. fruticosatend to favour a resource acquisition strategy to acquire more soil nutrients. On less stressful shady slopes, the above-ground organs ofD. fruticosaexhibited higher hydraulic efficiency and photosynthetic capacity, and adopted a resource acquisition strategy. Thus it seems the below- and above-ground functional traits ofD. fruticosain different habitats are coordinated to comprise the whole plant ecological response. Conclusion Our study clearly shows hydraulic functional traits and leaf photosynthesis characteristics play key roles in shrub encroachment. This research also provides new insights for understanding the adaptation strategies of plant functional traits under different habitats.
Increased nerve growth factor expression and osteoclast density are associated with subchondral bone marrow lesions in osteoarthritic knees
Authors: Aso Koji , Sugimura Natsuki , Wada Hiroyuki , deguchi Syo , Masahiko Ikeuchi 
Published: October 2023

Abstract

Background
Subchondral bone marrow lesions (BMLs) detected on MRI in knee osteoarthritis (OA) are associated with knee pain. However, the pain mechanisms of BMLs are unknown. Increased nerve growth factor (NGF) expression at the osteochondral junction and increased osteoclast density in subchondral bone appear to be key features associated with bone pain in knee OA. Therefore, in this study, we aimed to identify the association of NGF and osteoclasts with BMLs in knee OA.
Methods
Twenty tibial plateaus were obtained from patients undergoing total knee arthroplasty for medial knee OA with BML at the medial tibia plateau (MTP). Osteochondral tissue with and without BML from the weight-bearing area in the MTP and without BML from the weight-bearing area in the lateral tibial plateau (LTP) was collected. Osteoarthritis bone score, Mankin score, NGF expression and density of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts were compared among the three osteochondral tissues.
Results
The osteoarthritis bone score, total Mankin score, NGF-expressed area and osteoclast density of the subchondral bone with BML in MTP were significantly higher than those of subchondral bone without BML in MTP and subchondral bone without BML in LTP. The mean differences of NGF-expressed area and osteoclast density between subchondral bone with BML and without BML in MTP were 9.0% (95% confidence intervals (CI): 5.9–12.1%) and 0.6 osteoclasts/mm (95% CI: 0.3–0.9%), respectively. In sections from subchondral tissue with BML in MTP, NGF immunoreactivity was detected in multinucleate osteoclasts, fibroblast-like cells, mononuclear cells and cartilage islands in the bone marrow space.
Conclusions
Increased NGF expression and osteoclast density are associated with subchondral BMLs in knee OA. This study will contribute to understanding the mechanisms of BML-related pain in OA and identifying new therapeutic targets for the management of bone pain in knee OA.
AI-based Strategies in Breast Mass ≤ 2cm classification with Mammography and Tomosynthesis: A Retrospective Evaluation
Authors: Shao Zhenzhen , Hao Yujuan , Hu Congyi , Yu Ziling , Shen Yue , Gao Fei , Zhang Fandong , Ma Wenjuan , Zhou Qian , Chen Jingjing , Lu Hong 
Published: October 2023

Abstract
Purpose To evaluate the efficiency of digital mammography (DM) and combined digital breast tomosynthesis (DBT) on AI-based strategies for breast mass ≤ 2cm classification.
Methods DM and DBT images in 483 patients including 512 breast masses were acquired from November 2018 to November 2019. The radiomics and deep learning methods were employed to extract the breast mass features in images and finally for benign and malignant classification. The DM and combined DBT (DM+DBT) images were fed into radiomics and deep learning models to construct corresponding models, respectively. The area under the receiver operating characteristic curve (AUC) was estimated models performance. A comprehensive comparison of the subgroups AUCs of the best optimal model was calculated on age, tumor size, and breast density category.
Results In the testing dataset, the AUC of DM combined DBT by radiomics and deep learning models were 0.869 and 0.908, respectively. Compared with the DM model, the combined DBT models based on radiomics and deep learning both showed statistically significant higher AUCs (0.869 vs. 0.810, P<0.001, by radiomics; 0.908 vs. 0.867, P<0.001, by deep learning). The deep learning models present superior than the radiomics models in the experiments with only DM (P<0.001) and DM+DBT (P<0.003). The advantage of the deep learning model is especially prominent in patients with small masses less than 1cm, 20 to 40 years old, and dense breast.
Conclusions Deep learning model based on DM+DBT has a best diagnostic efficiency. AI-based stragies will play a major role in detecting early breast cancer in screening.
Effects of Management of Heavy Menstrual Bleeding of Patients Undergoing Haploidentical Hematopoietic Stem Cell Transplantation
Authors: Yang Xin , Han Ruxue , Zhang Feifeng , Jiang Xiaolin , Wang Chaohua 
Published: October 2023

Abstract
Objective Heavy menstrual bleeding (HMB) was an underestimated bleeding complications of hematopoietic stem cell transplantation (HSCT). The study aims to investigate the benefits of menstrual management in women undergoing HSCT.Methods Patients who had undergone HSCT in the Gynecological Endocrinology Clinic of Peking University People's Hospital were included in ten years. The data of hematology and menstruation of all participants had been collected. The management methods of intervention group include GnRHa, COC or low-dose mifepristone. Patients who did not receive management were included in the control group.Results There were 112 patients included in the intervention group and 218 patients included in the control group. There were 90.0% of patients presenting with HMB before HSCT. In the control group, 83.5% of patients experienced uterine bleeding in the LAFR, while only 22.3% did in the intervention group. The rate of recurrent uterine bleeding after HSCT was significantly higher in the control group (50.9% vs. 17.9%, P < 0.001). Patients who did not undergo menstrual management had a higher risk of uterine bleeding than those who did (OR = 18.12, P < 0.001).Conclusion Menstrual management significantly reduces the incidence of uterine bleeding in HSCT patients and acts as a protective factor against uterine bleeding in the LAFR.
Sexual differences in neuronal and synaptic properties across subregions of the mouse insular cortex
Authors: Iezzi Daniela , Cáceres-Rodríguez Alba , Strauss Benjamin , Chavis Pascale , Manzoni Olivier 
Published: October 2023

Abstract
Background
The insular cortex (IC) plays a pivotal role in processing interoceptive and emotional information, offering insights into sex differences in behavior and cognition. The IC comprises two distinct subregions: the anterior insular cortex (aIC), that processes emotional and social signals, and the posterior insular cortex (pIC), specialized in interoception and perception of pain. Pyramidal projection neurons within the IC integrate multimodal sensory inputs, influencing behavior and cognition. Despite previous research focusing on neuronal connectivity and transcriptomics, there has been a gap in understanding pyramidal neurons characteristics across subregions and between sexes.
Methods
Adult male and female C57Bl/6J mice were sacrificed and tissue containing the IC was collected for ex vivo slice electrophysiology recordings that examined baseline sex differences in synaptic plasticity and transmission within aIC and pIC subregions.
Results
Clear differences emerged between aIC and pIC neurons in both males and females: aIC neurons exhibited distinctive features such as larger size, increased hyperpolarization, and a higher rheobase compared to their pIC counterparts. Furthermore, we observed variations in neuronal excitability linked to sex, with male pIC neurons displaying a greater level of excitability than their female counterparts. We also identified region-specific differences in excitatory and inhibitory synaptic activity and the balance between excitation and inhibition in both male and female mice. Adult females demonstrated greater synaptic strength and maximum response in the aIC compared to the pIC. Lastly, synaptic long-term potentiation occurred in both subregions in males but was specific to the aIC in females.
Conclusions
We conclude that there are sex differences in synaptic plasticity and excitatory transmission in IC subregions, and that distinct properties of IC pyramidal neurons between sexes could contribute to differences in behavior and cognition between males and females.