bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSXI-6 The Effects Cattle Breed (Holstein Vs Angus × Holstein) on Growth Performance, Energetic Efficiency, and Carcass Characteristics of Calf-Fed Steers
Authors: Carvalho Pedro H V , Latack Brooke C , Ferraz Marcos V C , Nolasco Lester J R P , Meireles Willi R , Oliveira Heitor O M , Zinn Richard A 
Published: November 2023

Abstract
Objective of the current study was to evaluate the effects of cattle breed (Holstein vs Angus × Holstein) on growth performance, energetic efficiency, and carcass characteristics of calf-fed steers. Eighty Holstein (HOL), initial body weight (IBW) of 128.7 ± 3 kg, and 80 Angus × Holstein cross (CROS) with IBW of 127.1 ± 3 kg, were blocked by IBW and randomly assigned to 40 pens (4 steers/pen; 20 pens per breed). All steers were fed a similar steam-flaked corn-based diet (63.5% steam-flaked corn, 12.0% hay, 17.5% of dry distillers grains with solubles, 4.0% cane molasses, and 3.0% vitamin/mineral supplement). All cattle were slaughtered after 328 days of feeding. There was no effect (P ≥ 0.13) of cattle breed on final body weight (615 ± 22 kg) and overall average daily gain (1.48 ± 0.07 kg/day). However, calf-fed HOL steers had greater (P = 0.04) overall dry matter intake compared with calf-fed CROS steers (8.06 vs. 7.79 ± 0.39 kg/day; respectively). Therefore, calf fed CROS steers had greater (P ≤ 0.01) grain to feed ratio compared with calf-fed HOL steers (0.192 vs. 0.182 ± 0.005; respectively). Moreover, calf-fed CROS steers had greater (P &lt; 0.01) utilization of dietary net energy for maintenance (2.29 vs. 2.29 ± 0.06 Mcal/kg; respectively) and gain (1.51 vs 1.61 ± 0.06 Mcal/kg; respectively) compared with calf-fed HOL steers. Calf-fed CROS steers had greater (P ≤ 0.02) hot carcass weight (HCW; 386.3 vs. 375.2 ± 14.7 kg; respectively), dressing percentage (DP; 62.3 vs. 61.4 ± 1.05%; respectively), longissimus muscle area (LMA; 86.9 vs. 79.1 ± 4.83 cm2; respectively), backfat thickness (0.89 vs. 0.55 ± 0.12; respectively), and marbling score (538 vs. 449 ± 48; respectively). However, there was no effect (P = 0.26) of cattle breed on calculated USDA yield grade (2.94 ± 0.24). Moreover, there was no effect (P = 0.39) of cattle breed on incidence of liver abscesses. Therefore, cross breeding calf-fed Angus × Holstein increased HCW, DP, LMA, and marbling compared with purebred calf-fed Holstein steers. Moreover, regardless of cattle breed, calf-fed steers in the current study had only 3.75% of liver with abscess.
PSXI-7 Sensing of Defecation and Urination Events Through Carbon Dioxide Monitoring
Authors: Wright Ryan , Ganino Alyssa , White Robin R 
Published: November 2023

Abstract
The continued success of livestock production in grazing systems depends on the ability to manage lands sustainably. Non-point-source emissions, particularly defecation/urination events (DUE), are a major cause of soil degradation, runoff into water sources, and nutrient management issues. Monitoring of these emissions from livestock through wearable sensing could support more precision management, but is complicated by the need for individualized sensors, variable signal strength across topographies, and unreliable data transmission in remote locations. The objective of this study was to explore opportunities to use CO2 sensing as an indicator of DUE from cattle on pasture using data obtained from an open-source sensor. The use of tail sensors was implemented to monitor the DUE of horses at various times of the day, ranging from 8 am to 5 pm. The tail sensors were developed using a neoprene base attached to velcro straps that were placed around the tailhead. The sensors themselves were equipped with a SparkFun ESP32 Thing Plus microprocessor connected to a SparkFun CO2 sensor and a lithium ion rechargeable battery that was charged via micro USB (SparkFun Electronics, Niwot, CO). Sensors were placed on 4 horses for 6 hours each collection period and data collection occurred over 9 days. Horses were observed in real time and DUE were recorded at the time of occurrence. Data were collected from the sensors via a Dragino LoRa gateway and a mobile hotspot for wireless connection. Data analysis relied on visual data exploration and targeted assessment of associations using random forest regression, support vector machines, and extreme gradient boosting. The visual data exploration revealed that DUE occurred in much lower frequency than non-events, and so random oversampling and undersampling were used for each classification approach to attempt to improve accuracy. Although CO2 peaks often corresponded to DUE, they also frequently occurred in the absence of DUE. All classification algorithms showed poor accuracies (0.50 to 0.51), which were only marginally improved by over- (&lt; 0.51) and undersampling (&lt; 0.69). This preliminary assessment revealed considerable noise in sensing CO2 emissions in production settings, which may preclude usefulness in DUE sensing.
PSI-29 Effects of Dietary Intake on Amino Acid Composition of Histotroph (Uterine Luminal fluid) in Nonpregnant Cows: Implications for Pregnancy
Authors: Neville Tammi , King Layla , Borowicz Pawel P , Reynolds Larry P , Caton Joel S 
Published: November 2023

Abstract
When the developing embryo enters the uterus, before the placenta develops, the embryo receives nourishment from endometrially derived histotroph produced by the uterine glands that remain functional throughout gestation. Throughout pregnancy, this histotroph remains necessary for proper embryonic development. Maternal nutrition may affect histotroph composition resulting in altered embryo development. The objectives were to determine the effects of dietary intake on histotroph amino acid (AA) concentrations. Eighteen multiparous nonpregnant cows (initial BW = 597 kg) were housed at the North Dakota State University Animal Nutrition and Physiology Center (n = 6 cows/pen). Cows were individually fed a TMR once daily via a Calan feeding system and had ad libitum access to fresh water. Cows were randomly assigned to 1 of 2 treatments (n = 9/treatment): dietary intake designed to maintain body weight (CON) or dietary intake designed to lose body weight at a moderate rate (-0.7 kg/d; NEG), for 63 days. Slaughter group 1 contained 4 CON and 5 NEG cows, and group 2 contained 5 CON and 4 NEG cows. Cows were estrus-synchronized with the Co-Synch + CIDR protocol to ensure a common day of the estrous cycle at the time of slaughter (3 d after completion of the Co-Synch + CIDR protocol). When total uterine histotroph AA was calculated (luteal + non-luteal horns) and analyzed by treatment with group in the model, the differences were: CON &gt; NEG for 1) Trp as % of essential AA, and 2) Ser as % of total AA (P = 0.04 and 0.05, respectively). Tendencies for total tract histotroph were observed for Phe and Trp as a % of total AA (P = 0.07 and 0.06, respectively) for CON &gt; NEG. Luteal histotroph Ser as a % of total AA was greater (P = 0.05) for CON vs. NEG. When evaluating non-luteal histotroph, CON had greater (P &lt; 0.05) Cys as a % of total AA compared with NEG cows. Non-luteal histotroph Arg concentration tended (P = 0.10) to be greater for NEG vs. CON. These results demonstrate that feed intake affects cow AA supply in the uterine histotroph, and these AA (Trp, Ser, Phe, Cys and Arg) may function in critical nutrient pathways, reproductive tract vascularization, and antioxidant production, which are necessary for optimal fetal development.
PSII-25 Use of Energy Dispersive X-Ray Fluorescence for Determination of Indigestible Markers in Digesta Samples
Authors: King Mindy E , Foote Andrew P 
Published: November 2023

Abstract
Titanium dioxide and chromium ethylenediaminetetraacetic acid are commonly used as indigestible markers for determining passage rates of diets to aid in determination of digestibility. However, commonly used methodology involving acid digestions and utilizing inductively coupled plasma spectroscopy and atomic absorption spectroscopy for both Ti and Cr analysis involves hazardous chemicals, destruction of samples, and low sample throughput. The objective of this experiment was to develop and validate an accurate and precise method for measuring both Ti and Cr using energy dispersive X-ray fluorescence (ED-XRF). Energy dispersive X-ray fluorescence is an analytical technique used for analyzing elements in various sample types. The samples are added to the ED-XRF machine and irradiated with X-rays and the intensity of the X-rays emitted (termed fluorescent X-rays) are used for calculation of the concentration of the element. The method for Ti was constructed using fecal samples from cattle consuming three different diet types (finishing diet, dairy lactation diet, and grazing native range pasture). The Cr method was developed for rumen fluid analysis. To determine accuracy and precision of the method, samples were spiked with various amounts of Ti or Cr and measured for their respective compounds with percent recovery and inter- and intra-assay CV calculated thereafter. Additionally, a regression was performed using PROC REG in SAS 9.4 to determine the relationship between grams of Ti added and grams of Ti recovered for each diet type. The average recovery for Ti across all diet types was 100.3%, and the recovery for Cr in rumen fluid was 95.7%. The average inter- and inter-assay CV for Ti, across all diet types, were 9.70 and 2.16%, respectively. For Cr, the average inter- and inter-assay CV were 5.41 and 8.45%, respectively. Across diet type, the regression for grams of Ti added versus grams of Ti recovered indicated the recovery was not altered by the amount added (P ≤ 0.062). The ED-XRF method requires minimal additional chemicals, is cost-effective, and allows for sample preservation as well as a high throughput of samples. Our results indicate utilization of ED-XRF is an accurate and precise method for determination of Ti in feces and Cr in rumen fluid.
PSIII-30 Effects of a Bacillus-Based Direct-Fed Microbial on in Vitro Gas Production and Fiber Digestibility of Feedstuffs
Authors: Cappellozza Bruno I , Fantinati Paolo , Bodin Jean-Christophe , Joergensen Jens , Vyas Diwakar , Farooq Samia , La Lima ís , de Guzman Carlos , Arriola Kathy , Copani Giuseppe 
Published: November 2023

Abstract
Direct-fed microbials (DFM) support the health and performance of the herd through different modes of action, including improvements on nutrient digestibility. In fact, Bacillus spp. has been identified as an important enzyme-producing bacteria that could benefit overall nutrient utilization by the beef and dairy cattle herd. Based on this rationale, we hypothesized that incubation of a Bacillus-based DFM would improve nutrient digestibility of feedstuffs often fed to ruminants. Therefore, our objective was to evaluate the effects of a Bacillus-based DFM on in vitro nutrient digestibility of different single feedstuffs. Six individual feedstuffs were sampled from China and South Africa and used herein. Feedstuffs were incubated or not (CON) with a Bacillus-based DFM (DFM; BOVACILLUS; B. licheniformis and B. subtilis; 3.2 × 109 CFU/gram; Chr. Hansen A/S., Hørsholm, Denmark) over a 48-h in vitro period. The dose of DFM was adjusted to the volume of the flasks being used and to represent the commercial dose of 3 grams/head per day (9.6 × 109 CFU/head). In vitro gas production, dry matter (DM), and neutral detergent fiber (NDF) digestibility were evaluated at 24 and 48 h. Data were analyzed using PROC MIXED of SAS (version 9.4). Mean total in vitro gas production increased by 5.1 and 6.5% when DFM was incubated at 24 and 48 h, respectively (P &lt; 0.01). At 24 h, NDF digestibility was 12.6% greater for DFM-treated feedstuffs vs. CON (P = 0.02; 27.7 vs. 24.6% for DFM and CON, respectively), but no effects were observed on DM digestibility (P = 0.26). Conversely, 48-h DM and NDF digestibility were greater for DFM vs. CON (P ≤ 0.05), with improvements that were in the order of 3.7 and 15.1%, respectively. In conclusion, we observed greater in vitro fiber degradability and gas production of feedstuffs collected from China and South Africa over a 48-h period when the DFM BOVACILLUS was added at a dose representative of 9.6 × 109 CFU per head. Greater improvements in fiber degradability are likely due to the enzymes produced by the DFM BOVACILLUS.
PSIII-25 Fecal Ph Variables of Beef Steers Consuming Grower Or Finisher Diets Containing Saccharomyces Cerevisiae
Authors: Pessoni Juliana , Sarturi Jhones O , Silva Kaliu G , Johnson Bradley J , Rodrigues Barbara , Dos Reis Beatriz 
Published: November 2023

Abstract
The effects of live-yeast and diet type (grower or finisher) offered to beef steers on fecal pH variables were evaluated. Ruminally-cannulated Angus-crossbred steers (n = 8; BW = 550 ± 20 kg) were used in a duplicated 4 × 4 Latin Square design, on a 2 × 2 factorial treatment arrangement, with factors described as follows: A) presence of live-yeast [Saccharomyces cerevisiae (CNCM I-1077), at 2 × 1010 CFU/g); and B) diet type. Diets (DM basis) contained 3% yellow-grease and min/vitamin supplement-adjusted. The grower [40% low-quality alfalfa-hay (LQ-hay), 35% steam-flaked corn (SFC), 20% wet corn gluten feed (WCGF)] and finisher (65% SFC, 20% WCGF, 8% LQ-hay)] diets were offered to ad libitum intake, once daily at 0700 h. Live-yeast treatment and control (soybean meal as carrier only) were pre-weighed (0.25 g, as is) within gel-capsules and kept at room temperature until introduced directly into the rumen (via ruminal cannula) twice daily (0700 and 1800 h). Four periods of 35 d (28 d of adaptation and 7 d of collection) were used. Fresh fecal samples (50 ± 1 g, as is) were collected via rectal finger-stimulation 4 times per day (every 6 h), during 6 consecutive days, while sampling time was adjusted by advancing 1 h daily so that all the 24 h period after feeding became represented by collections. Samples were suspended with deionized water (to complete 100 g, total) into a plastic container with cap and immediately measured using a pH electrode. The statistical model used animal as experimental unit, the fixed effects of diet, live-yeast, and diet × live-yeast. Square, period, and animal(square) were used as random effects, except by pH average (repeated measures), in which animal(treatment) was also included. For fecal pH average, no 3-way, treatment × time, or diet × time interactions were observed (P ≥ 0.86), except by a treatment × diet interaction, in which animals offered live-yeast on a grower diet had greater (P = 0.04) fecal pH compared with those offered live-yeast on a finisher diet (6.63 vs. 6.55). Regardless of treatments, collection time was meaningful (P = 0.01), where the greatest fecal pH average (6.70) was reached at 14 h, while the least (5.52) was reached at 8, 15, and 22 h after feeding. The fecal pH average across all treatments was 6.58, and it was used to calculate area and time under such threshold. The area (147, min × pH-delta) and the time (730 min) under the curve (pH 6.58), maximum (6.98), minimum (6.22), and the pH magnitude of change (0.76) were not affected (P ≥ 0.37) by treatments. Fecal pH was cyclic and seemed to be positively affected by dietary live-yeast inclusion when offered to beef steers consuming a grower diet.
PSIII-29 A Novel Direct-Fed Microbial Supports in Vitro Intestinal Integrity Under Inflamed Conditions
Authors: Boll Erik Juncker , Cappellozza Bruno I , Copani Giuseppe 
Published: November 2023

Abstract
Ruminants are often exposed to pathogenic and non-pathogenic challenges during daily handling procedures and management activities. These responses, in turn, may trigger the release of pro-inflammatory compounds that can have negative effects on the host. Based on this rationale, we hypothesized that 1) pro-inflammatory cytokines (TNF-α and IFN-γ) would compromise the in vitro gut barrier integrity, and 2) a novel direct-fed microbial (DFM) formulation would support the gut barrier integrity under inflammatory conditions. Therefore, our objective was to evaluate the ability of a novel DFM mixture to support barrier integrity of intestinal epithelial cell monolayers in the absence or presence of TNF-α and IFN-γ by measuring transepithelial electrical resistance (TEER). Human cancer-derived epithelial intestinal Caco-2 cell monolayers were seeded on transwells for approximately 20 days. A DFM mixture containing Lactobacillus animalis, Propionibacterium freudenreichii, Bacillus licheniformis, and B. subtilis (BOVAMINE DEFEND Plus; Chr. Hansen A/S, Hørsholm, Denmark) at 1.0 × 108 CFU/transwell was then administered to the apical side of the cell monolayers. Then, two hours later a cocktail containing TNF-α / IFN-γ at a 10:1 ratio (100:10 ng/mL, respectively) was added to the basolateral side after which TEER was measured over a 16-h period and used to calculate the area under the curve (AUC). Following the administration of the pro-inflammatory challenge, TEER was decreased, reaching its least concentrations at 5.5 h post-challenge, whereas overall AUC decreased by 21.4% compared with non-challenged control cells. The DFM mixture by itself was able to support the integrity of the intestinal cells by stimulating TEER increase over time and increasing overall AUC vs. non-treated cells (P &lt; 0.01). Moreover, when cells receiving the DFM were challenged with the pro-inflammatory cocktail, TEER concentrations were still above 100% and no decrease was observed over the evaluation period (16 h). Moreover, when compared with non-DFM-treated but challenged cells, AUC was greater for DFM vs. challenged control cells (P &lt; 0.05). In conclusion, these data support our hypothesis that a DFM mixture containing L. animalis, P. freudenreichii, B. licheniformis, and B. subtilis (BOVAMINE DEFEND Plus) supports the gut barrier integrity with or without the pro-inflammatory challenge.
PSVI-22 Effect of Creep-Feeding on Feeding Behavior of Nellore Calves
Authors: Sousa Luciana Melo , Bisio Gustavo Henrique Maciel , Garcia Anna Lidia Francino , de Queiroz Amanda Caroliny Marques , de Porcionato Marco Aurélio Felício , de Resende Flávio Dutra , Siqueira Gustavo Rezende 
Published: November 2023

Abstract
The study aimed to evaluate the effect of creep-feeding on feeding behavior of beef calves, during nursing phase. The experiment lasted 170 days; from December/2021 to May/2022, the calves were born in September/2021 and creep-feeding supplementation started from approximately 70 days of life until weaning. The feeding behavior was evaluated twice on d 70 and d 120 post creep-feeding). The experimental design used was a randomized block [blocked by body weight (BW) of calves]. The experimental units were the paddocks (8 units per treatment, ten replicates per paddock), totalling 156 pairs cow/calf (86 pairs of male calves and 70 pairs of female calves). Two paddocks per treatment and 8 pairs per paddock were sorted for behavior analysis, totalling 32 pairs cow/calf (16 pairs of male calves and 16 pairs of female calves). The treatments consisted of creep-feeding or not creep-feeding the calves: control treatment (CONT; mineral suppement ad libitum) and creep-feeding treatment (CREEP; energetic protein supplement; 5 g/kg of BW· animal-1· /day-1). There was mineral supplement ad libitum for all the dams. The pairs cow/calf were kept in an area composed of Urochloa brizantha cv. Marandu. and idleness, suckling, grazing and creep-feeding or mineral supplementation were evaluated. The data were analyzed using the SAS PROC MIXED. There was no difference in feeding behavior of calves at 70 days of creep-feeding for idleness (P = 0.80), grazing (P = 0.59) and suckling (P = 0.30; Figure 1). Creep-feeding calves spent 14 minutes longer on supplementation than non-creep-feeding calves (P &lt; 0.01). At 120 days of creep-feeding (180 days of age), calves that received creep feeding supplementation spent less time grazing (difference of 67 min; P &lt; 0.01), more time on supplementation (+28 min; P &lt; 0.01) and idleness (+42 min; P = 0.05) and similar time on suckling (P = 0.76; Figure 2).There was no difference in any of the variables at different times (P &gt;0.08) for the dams (Figure 3). Animals in creep-feeding spend less time grazing but suckling in a similar amount of time compared with those that consumed only mineral supplement, which corroborates with the results on milk yield of dams, which were similar for both treatments.
PSVII-28 Effects of Adding Either Fibrolytic Enzymes Or Sodium Butyrate to Feedlot Diets on Rumen Parameters of Angus Cattle
Authors: Casali Daniel M , Silva Leandro A F , Lelis Ana Laura J , Souza Johnny M , Magalhães Eduardo J S , Silva Mikaeli A S , Belini Matheus S , Mathias Rafael F , Melo Tarcisio L A , Avila Jorge A V , Millen Danilo D 
Published: November 2023

Abstract
This study conducted at São Paulo State University feedlot, Dracena campus, Brazil, was designed to evaluate the addition of either sodium butyrate (BUT) or exogenous fibrolytic enzymes (ENZ) on dry matter intake (DMI), ruminal pH, short-chain fatty acids (SCFA), and rumen disappearance rate of Angus cattle. Three Angus steers, cannulated in the rumen (+ 690 kg), were used in a replicated 3 x 3 Latin square, resulting in 6 experimental periods, in a total of 203 days of study. Each experimental period lasted 28 days; 14 days of adaptation diets, and 14 days of the finishing diet containing 85% concentrate (71% finely-ground corn). Cattle were given a 7-day washout interval between periods, where they were allowed to graze. The experimental diets fed to the animals were different only with respect to the feed additives used, according to the treatments: Control (CTL; no feed additives added), ENZ [0.01% of diet dry matter (DM)], and BUT (0.3% and 0.1% of diet DM during adaptation and finishing phases, respectively). The DMI was measured daily, and rumen pH and temperature were measured by data loggers on days 25 and 26 of each experimental period. The rumen fluid samples were collected at 0, 4, 8, and 12 hours after feeding on day 26 of each period to determine the molar proportion of SCFA. The rumen of the animals was emptied on day 27 at 1100 h (3 hours after feeding) and day 28 at 0800 h (before feeding) in each experimental period. The rumen contents were separated into solid and liquid fractions and then weighed. Based on the solid and liquid mass values obtained, as well as the DM intake, the rumen disappearance rate was calculated and expressed in %/h. Data were analyzed by PROC MIXED of SAS (2003), using Tukey's test to compare means when appropriate, considering P ≤ 0.05 as significant. The DMI was not affected (P &gt; 0.76) by treatments in this study. Likewise, no treatment effect was observed on minimum, maximum, and mean ruminal pH (P &gt; 0.23). The pH time below 6.2, 5.6, and 5.2 was not affected by treatments (P &gt; 0.45), as well as pH area under 6.2, 5.6, and 5.2 (P &gt; 0.55). Rumen temperature was lower in cattle fed ENZ when compared with those fed CON and BUT (39.63°C vs. 40.12°C and 40.02°C, respectively; P = 0.04). Furthermore, the molar proportions of SCFA were not affected by treatments (P &gt; 0.71). Cattle consuming ENZ increased ruminal disappearance rate (%/h) when compared with CTL-fed cattle (8.61 vs 6.36; P = 0.05). Thus, the addition of BUT to feedlot diets did not cause any impact on ruminal fermentation parameters; however, the use of ENZ promoted small benefits that deserve further investigation.