bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photo‐induced Oriented Crystallization of Intracellular Nanocrystals Based on Phase Separation for Diagnostic Bioimaging and Analysis
Authors: Liu Hao , Liu Zhiming , Xiao Jiang , Liu Xiaohui , Jiang Hui , Wang Xuemei 
Published: January 2024

AbstractBiomineral crystals form complex nonequilibrium structures based on the multistep nucleation theory, via transient amorphous precursors. However, the intricate nature of the biological system results in the inconsistent frequency of nucleation and crystallization, which making it problematic to obtain homogeneous nanocrystals, limits their application in biomedicine. Here, it is reported that homogeneous nanocrystals of photoinduced oriented crystallization with protein coronas are based on intracellular liquid–liquid phase separation for in situ analysis and mapping of surface‐enhanced Raman spectroscopy (SERS). Near‐infrared light promotes the formation of intracellular dense phases, accelerates the nucleation of gold atoms at secondary structure sites of proteins, and promotes the growth of crystals. Homogeneous gold nanocrystals with stable SERS signals can be used to analysis different cell cycles and acquire in situ molecular information of metastatic tumor cells. Of note are tag molecule is embedded in protein coronas of gold nanocrystals to enable the mapping of patient tumor tissue samples and the portable recognition of tumor cells. Thus, this study proposes a new strategy for biomineralization of intracellular homogeneous gold nanocrystals and its potential application.
Self‐Supplied Reactive Oxygen Species‐Responsive Mitoxantrone Polyprodrug for Chemosensitization‐Enhanced Chemotherapy under Moderate Hyperthermia
Authors: Zhang Hongjie , Wang Jing , Wu Ruiying , Zheng Benyan , Sang Yanxiang , Wang Bibo , Song Lei , Hu Yuan , Ma Xiaopeng 
Published: January 2024

AbstractCurrently, the secondary development and modification of clinical drugs has become one of the research priorities. Researchers have developed a variety of TME‐responsive nanomedicine carriers to solve certain clinical problems. Unfortunately, endogenous stimuli such as reactive oxygen species (ROS), as an important prerequisite for effective therapeutic efficacy, are not enough to achieve the expected drug release process, therefore, it is difficult to achieve a continuous and efficient treatment process. Herein, a self‐supply ROS‐responsive cascade polyprodrug (PMTO) is designed. The encapsulation of the chemotherapy drug mitoxantrone (MTO) in a polymer backbone could effectively reduce systemic toxicity when transported in vivo. After PMTO is degraded by endogenous ROS of the TME, another part of the polyprodrug backbone becomes cinnamaldehyde (CA), which can further enhance intracellular ROS, thereby achieving a sustained drug release process. Meanwhile, due to the disruption of the intracellular redox environment, the efficacy of chemotherapy drugs is enhanced. Finally, the anticancer treatment efficacy is further enhanced due to the mild hyperthermia effect of PMTO. In conclusion, the designed PMTO demonstrates remarkable antitumor efficacy, effectively addressing the limitations associated with MTO.
Salmon trade duration: The application of firm‐level trade transaction data from the Norwegian salmon industry
Authors: Jaghdani Tinoush Jamali , Johansen Ulf , Thakur Maitri , Glauben Thomas 
Published: January 2024

AbstractSeafood products are mainly provided through trade to international markets and Norway is the major international player for the export of salmon products. It is argued that fresh or chilled salmon, which are perishable products and Norway's main seafood export, need stable trade partners to safely and properly reach consumption markets. In this study, the firm‐to‐firm level trade transactional data for both exporters and importers for the 2005–2018 period was utilized for a trade duration analysis. The Kaplan–Meier survival estimators, Cox proportional hazard model, and frailty model were used to study the stability of trade relations between the partners through survival and hazard models. The study considers the heterogeneous firm trade model along with a theoretical framework. The results show that, on average, the trade duration for the salmon trade between firms is 2.39 years, which is relatively low for a perishable product such as salmon. It shows that the market entry and exit is relatively fast, and that the survival rate between the EU as a major demand market and non‐EU countries is not very different. However, core and periphery markets can be defined by considering the survival rate, as major processing hubs in the EU, such as Poland and France as core markets, have higher survival rates compared with other countries that can be considered peripheries. Both extensive and intensive margins were found to reduce the hazard ratio. Different hazard ratios were also found between different types of exporter or ones utilizing different means of transport. We conclude that the trade duration between the partners can be increased if the competition in the market increases, which is probably possible in markets like the United States where other exporters such as Chile are available.
Harmonizing florbetapir and PiB PET measurements of cortical Aβ plaque burden using multiple regions‐of‐interest and machine learning techniques: An alternative to the Centiloid approach
Authors: Chen Kewei , Ghisays Valentina , Luo Ji , Chen Yinghua , Lee Wendy , Wu Teresa , Reiman Eric M. , Su Yi 
Published: January 2024

AbstractINTRODUCTIONMachine learning (ML) can optimize amyloid (Aβ) comparability among positron emission tomography (PET) radiotracers. Using multi‐regional florbetapir (FBP) measures and ML, we report better Pittsburgh compound‐B (PiB)/FBP harmonization of mean‐cortical Aβ (mcAβ) than Centiloid.METHODSPiB‐FBP pairs from 92 subjects in www.oasis‐brains.org and 46 in www.gaain.org/centiloid‐project were used as the training/testing sets. FreeSurfer‐extracted FBP multi‐regional Aβ and actual PiB mcAβ in the training set were used to train ML models generating synthetic PiB mcAβ. The correlation coefficient (R) between the synthetic/actual PiB mcAβ in the testing set was assessed.RESULTSIn the testing set, the synthetic/actual PiB mcAβ correlation R = 0.985 (R2 = 0.970) using artificial neural network was significantly higher (p ≤ 6.6e‐4) than the FBP/PiB correlation R = 0.927 (R2 = 0.860), improving total variance percentage (R2) from 86% to 97%. Other ML models such as partial least square, ensemble, and relevance vector regressions also improved R (p = 9.677e−05/0.045/0.0017).DISCUSSIONML improved mcAβ comparability. Additional studies are needed for the generalizability to other amyloid tracers, and to tau PET.Highlights
Centiloid is a calibration of the amyloid scale, not harmonization.
Centiloid unifies the amyloid scale without improving inter‐tracer association (R2).
Machine learning (ML) can harmonize the amyloid scale by improving R2.
ML harmonization maps multi‐regional florbetapir SUVRs to PiB mean‐cortical SUVR.
Artificial neural network ML increases Centiloid R2 from 86% to 97%.
Amino‐λ3‐iodane‐Enabled Electrophilic Amination of Arylboronic Acid Derivatives
Authors: Kiyokawa Kensuke , Kawanaka Kazuki , Minakata Satoshi 
Published: January 2024

AbstractIn this report, we describe the use of amino‐λ3‐iodanes in the electrophilic amination of arylboronic acids and boronates. Iodine(III) reagents with transferable amino groups, including one with an NH2 group, were synthesized and used in the amination, allowing the synthesis of a wide range of primary and secondary (hetero)arylamines. Mechanistic studies by DFT calculations indicate that the reaction proceeds through an electrophilic amination process from a tetravalent borate complex with a B−N dative bond.
Vitamin D and Marine n‐3 Fatty Acids for Autoimmune Disease Prevention: Outcomes Two Years After Completion of a Double‐Blind, Placebo‐Controlled Trial
Authors: Costenbader Karen H. , Cook Nancy R. , Lee I‐Min , Hahn Jill , Walter Joseph , Bubes Vadim , Kotler Gregory , Yang Nicole , Friedman Sonia , Alexander Erik K. , Manson JoAnn E. 
Published: January 2024

ObjectiveIn the 5.3‐year randomized, 2 × 2 factorial, double‐blind, placebo‐controlled Vitamin D and Omega‐3 Trial (VITAL), vitamin D supplementation reduced autoimmune disease (AD) incidence (hazard ratio [HR] 0.78, 95% confidence interval [CI] 0.61–0.99). Omega‐3 (n‐3) fatty acid supplementation showed a statistically nonsignificant reduction (HR 0.85, 95% CI 0.67–1.08). We aimed to confirm further AD cases arising during and after randomization and assess sustained effects with two years of postintervention observation.MethodsOf the 12,786 men aged ≥50 and 13,085 women aged ≥55 initially randomized, we observed surviving and willing participants for two more years. We continued to confirm annual participant‐reported new AD by medical record review. Cox models calculated HRs for all confirmed incident AD, (and secondary endpoints, including probable cases, and individual ADs), during the observational and randomized periods.ResultsA total of 21,592 participants (83.5%) were observed for two more years; 514 participants developed incident confirmed AD (236 since prior report), of whom 255 had been randomized to vitamin D versus 259 to vitamin D placebo (HR 0.98 [95% CI 0.83–1.17] at 7 years). AD was confirmed in 234 participants initially randomized to n‐3 fatty acids versus 280 randomized to its placebo (HR 0.83 [95% CI 0.70–0.99] at 7 years). Of newly confirmed cases, 65 had onset during randomization; their inclusion changed randomized results as follows: HR 0.85 (95% CI 0.70–1.04) for vitamin D and HR 0.87 (95% CI 0.71–1.06) for n‐3 fatty acids.ConclusionTwo years after trial termination, the protective effects of 2000 IU/day of vitamin D dissipated, but 1,000 mg/day of n‐3 fatty acids had a sustained effect in reducing AD incidence.
Myositis‐Associated Autoantibodies in Patients With Juvenile Myositis Are Associated With Refractory Disease and Mortality
Authors: Sherman Matthew A. , Noroozi Farhadi Payam , Pak Katherine , Trieu Edward P. , Sarkar Kakali , Targoff Ira N. , Neely Megan L. , Mammen Andrew L. , Rider Lisa G. ,   for the Childhood Myositis Heterogeneity Collaborative Study Group
Published: January 2024

ObjectiveMyositis‐associated autoantibodies (MAAs) have been associated with overlap myositis, certain disease manifestations such as interstitial lung disease (ILD), and worse prognosis in the idiopathic inflammatory myopathies. MAAs overall remain largely uncharacterized in patients with juvenile‐onset myositis. Moreover, it is unknown whether the number of MAAs is associated with disease severity.MethodsPatients with juvenile myositis in cross‐sectional natural history studies who underwent testing for myositis autoantibodies were included. Demographics, myositis autoantibodies, clinical characteristics, medications received, and outcomes of those with and without MAAs were compared. Multivariable logistic regression was performed to determine whether the number of MAAs detected was associated with severe disease features.ResultsAmong 551 patients, 36% had an MAA and 13% had more than one MAA. Among those who were MAA positive, there was a higher frequency of overlap myositis (18% vs 5.9%, P < 0.001). MAA positivity was associated with certain clinical features, including Raynaud phenomenon (odds ratio [OR] 2.44, 95% confidence interval [CI] 1.41–4.28) and ILD (OR 3.43, 95% CI 1.75–6.96), as well as a chronic disease course (OR 1.72, 95% CI 1.10–2.72) and mortality (OR 3.76, 95% CI 1.72–8.43). The number of MAAs was also associated with mortality (OR 1.83, 95% CI 1.16–2.86).ConclusionMAAs were prevalent in a large cohort of patients with juvenile myositis. ILD, refractory disease, and mortality were associated with MAA positivity. Prospective studies are needed to determine whether early detection of MAAs may lead to improved outcomes for patients with juvenile myositis.
Flatness‐based sliding mode control for stabilizing a spherical pendulum on a quadrotor
Authors: Martinez‐Vasquez Adrian Humberto , Castro‐Linares Rafael 
Published: January 2024

AbstractThis paper presents the problem of transporting a suspended load by a quadrotor. A full model considering the quadrotor and the dynamics of the suspended load, in a three‐dimensional space, is proposed considering as control inputs the torques and the thrust force due to the motors. The solution proposed consists of simple control strategies based on the tangent linearization of the model, which is controllable and therefore flat. A sliding mode control technique is developed for the thrust force and torques associated with the Euler angles in order to track a desired trajectory of the vehicle in a three‐dimensional space with minimum oscillation of the suspended load. The control strategy results to be relatively simple to implement and achieves local stability of the tracking errors, as well as robustness to internal nonlinearities, which are neglected in the linearization process and other external disturbances. The attractiveness of the sliding surfaces and the stability of the tracking errors are formally studied using Lyapunov stability theory. Simulation results are given to show the performance of the proposed control strategy.
Adaptive robust control of nonaffine nonlinear systems via an improved event‐triggered mechanism
Authors: Wan Quan , Pan Yingnan , Lu Qing , Jing Yanhui 
Published: January 2024

AbstractThis paper aims to address the problem of event‐based adaptive robust control for a class of nonaffine nonlinear systems against actuator faults and unknown deception attacks. Firstly, different from the widely used relative threshold triggering mechanism, an improved event‐triggered mechanism with the time‐varying function threshold is devised for effectively saving more communication costs between controller and actuator. Then, based on a class of modified filter‐based coordinate transformations and the improved event‐triggered mechanism, the intermediate virtual control laws are devised to construct actual controllers, which not only guarantees robust stabilization of the system but also relaxes the universal assumptions on control coefficients and attack weights. Besides, the design challenges resulting from the presence of coupling terms in the nonaffine nonlinear system are successfully overcome by virtue of mean value theorem and the property of fuzzy basis function. Theoretical analysis proves that all signals of the closed‐loop system are semiglobally uniformly ultimately bounded (SUUB) and the Zeno behavior can be averted. Finally, the effectiveness of the proposed strategy is verified via comprehensive simulation results.
Multivariate simulation‐based forecasting for intraday power markets: Modeling cross‐product price effects
Authors: Hirsch Simon , Ziel Florian 
Published: January 2024

AbstractIntraday electricity markets play an increasingly important role in balancing the intermittent generation of renewable energy resources, which creates a need for accurate probabilistic price forecasts. However, research to date has focused on univariate approaches, while in many European intraday electricity markets all delivery periods are traded in parallel. Thus, the dependency structure between different traded products and the corresponding cross‐product effects cannot be ignored. We aim to fill this gap in the literature by using copulas to model the high‐dimensional intraday price return vector. We model the marginal distribution as a zero‐inflated Johnson's distribution with location, scale, and shape parameters that depend on market and fundamental data. The dependence structure is modeled using copulas, accounting for the particular market structure of the intraday electricity market, such as overlapping but independent trading sessions for different delivery days and allowing the dependence parameter to be time‐varying. We validate our approach in a simulation study for the German intraday electricity market and find that modeling the dependence structure improves the forecasting performance. Additionally, we shed light on the impact of the single intraday coupling on the trading activity and price distribution and interpret our results in light of the market efficiency hypothesis. The approach is directly applicable to other European electricity markets.
Mapping active civic learning in primary schools across England—A call to action
Authors: Body Alison , Lau Emily , Cunliffe Jack , Cameron Lindsey 
Published: January 2024

AbstractEncouraging children to become ‘good citizens’ who positively contribute towards society through charitable and philanthropic action as part of their civic participation has become a core focus of policy and practice. Yet the opportunities afforded to children for active civic learning within primary education remain under‐researched. This article presents findings from a multi‐survey study that seeks to unpick ‘what’ and ‘how’ active civic learning is happening in primary schools across England. By mapping active civic learning across the country, we find that these opportunities are unequally dispersed. Specifically, from an early age, children from more affluent backgrounds are more likely to be prepared for active civic engagement, orientated around ideas of social justice, than those from disadvantaged backgrounds. This raises significant challenges for education policy and practice and calls for greater attention to be paid to civic learning for all children in early and middle childhood.
Synthesis, thermoluminescence characterizations, and photon attenuation parameters of MgAl2O4 structure doped with different ions
Authors: Qaid Saif M. H. , Asemi Nassar N. , Ahmed Abdullah , Alkadi Muath , Alawsh Sufyan 
Published: January 2024

AbstractIn this work, different ion co‐doped Mg1‐xAl2O4 nanophosphors, coded as M5Cr‐5LaA, M5Cr‐5CuA, M0.07Si‐0.03CeA, and M0.05Ti‐0.05LaA, where 5Cr‐5La, 5Cr‐5Cu, 0.07Si‐0.03Ce, and 0.05Ti‐0.05La representing the added ions (mol%), were prepared using the sol–gel method. Phase structure, transmission electron microscope (TEM) images, and element feasibility were checked using X‐ray diffraction, TEM analysis, and energy dispersive X‐ray (EDX) spectroscopy. Their thermoluminescence (TL) response was checked using a 5 Gy γ‐test dose. The M0.05Ti‐0.05LaA sample was found to be best for the TL response with an ~1.1 times response compared with that of the MTS‐700 commercial detector. A wide range of dose–responses for the M0.05Ti‐0.05LaA sample was found up to a 20 Gy γ‐dose with the lowest detectable dose of ⁓23 μGy. Photon attenuation parameter results were Zeff ⁓10, which mean that the M0.05Ti‐0.05LaA sample could be considered as a near tissue equivalent material. Due to this study, the M0.05Ti‐0.05LaA sample can be considered as a promising detector for application in personal and medical dosimetric monitoring.
Caffeic acid phenethyl ester mediates apoptosis in serum‐starved HT29 colon cancer cells through modulation of heat shock proteins and MAPK pathways
Authors: Yahya Showket , Sulaiman Mahaboob Khan , Sudhandiran Ganapasam 
Published: January 2024

AbstractColorectal cancer (CRC) is among the most prevalent gastrointestinal cancers of epithelial origin worldwide, with over 2 million cases detected every year. Emerging evidence suggests a significant increase in the levels of inflammatory and stress‐related markers in patients with CRC, indicating that oxidative stress and lipid peroxidation may influence signalling cascades involved in the progression of the disease. However, the precise molecular and cellular basis underlying CRC and their modulations during bioactive compound exposure have not yet been deciphered. This study examines the effect of caffeic acid phenethyl ester (CAPE), a natural bioactive compound, in HT29 CRC cells grown under serum‐supplemented and serum‐deprived conditions. We found that CAPE inhibited cell cycle progression in the G2/M phase and induced apoptosis. Migration assay confirmed that CAPE repressed cancer invasiveness. Protein localisation by immunofluorescence microscopy and protein expression by western blot analysis reveal increased expressions of key inflammatory signalling mediators such as p38α, Jun N‐terminal kinase and extracellular signal‐regulated kinase (ERK) proteins. Molecular docking data demonstrates that CAPE shows a higher docking score of −5.35 versus −4.59 to known p38 inhibitor SB203580 as well as a docking score of −4.17 versus −3.86 to known ERK1/2 inhibitor AZD0364. Co‐immunoprecipitation data reveals that CAPE treatment effectively downregulates heat shock protein (HSP) expression in both sera‐supplemented and limited conditions through its interaction with mitogen‐activated protein kinase 14 (MAPK14). These results suggest that stress induction via serum starvation in HT29 CRC cells leads to the induction of apoptosis and co‐ordinated activation of MAPK‐HSP pathways. Molecular docking studies support that CAPE could serve as an effective inhibitor to target p38 and MAPK compared to their currently known inhibitors.
Design, Synthesis, and Evaluation of An Anti‐trypanosomal [1,2,4]Triazolo[1,5‐a]pyrimidine Probe for Photoaffinity Labeling Studies
Authors: Lucero Bobby , Francisco Karol R. , Varricchio Carmine , Liu Lawrence J. , Yao Yuemang , Brancale Andrea , Brunden Kurt R. , Caffrey Conor R. , Ballatore Carlo 
Published: January 2024

AbstractStudies have shown that depending on the substitution pattern, microtubule (MT)‐targeting 1,2,4‐triazolo[1,5‐a]pyrimidines (TPDs) can produce different cellular responses in mammalian cells that may be due to these compounds interacting with distinct binding sites within the MT structure. Selected TPDs are also potently bioactive against the causative agent of human African trypanosomiasis, Trypanosoma brucei, both in vitro and in vivo. So far, however, there has been no direct evidence of tubulin engagement by these TPDs in T. brucei. Therefore, to enable further investigation of anti‐trypanosomal TPDs, a TPD derivative amenable to photoaffinity labeling (PAL) was designed, synthesized, and evaluated in PAL experiments using HEK293 cells and T. brucei. The data arising confirmed specific labeling of T. brucei tubulin. In addition, proteomic data revealed differences in the labeling profiles of tubulin between HEK293 and T. brucei, suggesting structural differences between the TPD binding site(s) in mammalian and trypanosomal tubulin.
Towards 2D Borane Chemistry in Hexagonal Cyclic Compounds
Authors: López‐Sánchez Rubén , Ferrer Maxime , Oliva‐Enrich Josep M. , Alkorta Ibon , Elguero José 
Published: January 2024

AbstractA comprehensive comparison between known benzene mono‐substituted compounds R−Ph and the corresponding isoelectronic unknown R‐cyclohexaborane(12) molecules is carried out from a geometric and electronic structure point of view, with R={H, BH2, CH3, NH2, OH, F ; AlH2, SiH3, PH2, SH, Cl ; NO2, OCH3}. We suggest new chemical names for the 2D borane compounds and analyze the geometric and electronic structure carbon vs. boron comparatives by means of HOMO‐LUMO gaps, bonding schemes, electron density topological properties and predicted NMR chemical shifts. The predictions on the properties in planar hexagonal cyclic boranes may help in the design of synthesis procedures for these yet‐unkown compounds.