bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between trajectories of dietary iron intake and risk of type 2 diabetes mellitus: evidence from a prospective cohort study
Authors: Wang Ruoting , Liu Yingxin , Thabane Lehana , Olier Ivan , Li Likang , Ortega-Martorell Sandra , Lip Gregory Y.H. , Li Guowei 
Published: February 2024

Abstract
Background
The association between dietary iron intake and the risk of type 2 diabetes mellitus (T2DM) remains inconsistent. In this study, we aimed to investigate the relationship between trajectories of dietary iron intake and risk of T2DM.

Methods
This study comprised a total of 61,115 participants without a prior T2DM from the UK Biobank database. We used the group-based trajectory model (GBTM) to identify different dietary iron intake trajectories. Cox proportional hazards models were used to evaluate the relationship between trajectories of dietary iron intake and risk of T2DM.

Results
During a mean follow-up of 4.8 years, a total of 677 T2DM events were observed. Four trajectory groups of dietary iron intake were characterized by the GBTM: trajectory group 1 (with a mean dietary iron intake of 10.9 mg/day), 2 (12.3 mg/day), 3 (14.1 mg/day) and 4 (17.6 mg/day). Trajectory group 3 was significantly associated with a 38% decreased risk of T2DM when compared with trajectory group 1 (hazard ratio [HR] = 0.62, 95% confidence interval [CI]: 0.49–0.79), while group 4 was significantly related with a 30% risk reduction (HR = 0.70, 95% CI: 0.54–0.91). Significant effect modifications by obesity (p = 0.04) and history of cardiovascular disease (p < 0.01) were found to the relationship between trajectories of dietary iron intake and the risk of T2DM.

Conclusions
We found that trajectories of dietary iron intake were significantly associated with the risk of T2DM, where the lowest T2DM risk was observed in trajectory group 3 with a mean iron intake of 14.1 mg/day. These findings may highlight the importance of adequate dietary iron intake to the T2DM prevention from a public health perspective. Further studies to assess the relationship between dietary iron intake and risk of T2DM are needed, as well as intervention studies to mitigate the risks of T2DM associated with dietary iron changes.
LDLR gene rearrangements in Czech FH patients likely arise from one mutational event
Authors: Konečná Kateřina , Zapletalová Petra , Freiberger Tomáš , Tichý Lukáš 
Published: February 2024

Abstract
Background
Large deletions and duplications within the low-density lipoprotein receptor (LDLR) gene make up approximately 10% of LDLR pathogenic variants found in Czech patients with familial hypercholesterolemia. The goal of this study was to test the hypothesis that all probands with each rearrangement share identical breakpoints inherited from a common ancestor and to determine the role of Alu repetitive elements in the generation of these rearrangements.

Methods
The breakpoint sequence was determined by PCR amplification and Sanger sequencing. To confirm the breakpoint position, an NGS analysis was performed. Haplotype analysis of common LDLR variants was performed using PCR and Sanger sequencing.

Results
The breakpoints of 8 rearrangements within the LDLR gene were analysed, including the four most common LDLR rearrangements in the Czech population (number of probands ranging from 8 to 28), and four less common rearrangements (1–4 probands). Probands with a specific rearrangement shared identical breakpoint positions and haplotypes associated with the rearrangement, suggesting a shared origin from a common ancestor. All breakpoints except for one were located inside an Alu element. In 6 out of 8 breakpoints, there was high homology (≥ 70%) between the two Alu repeats in which the break occurred.

Conclusions
The most common rearrangements of the LDLR gene in the Czech population likely arose from one mutational event. Alu elements likely played a role in the generation of the majority of rearrangements inside the LDLR gene.
Effects of intensive care unit quality assessment on changes in medical staff in medical institutions and in-hospital mortality
Authors: Kim Seungju , Kim Gui Ok , Lee Syalrom , Kwon Yong Uk 
Published: February 2024

Abstract
Background
Quality assessments are being introduced in many countries to improve the quality of care and maintain acceptable quality levels. In South Korea, various quality assessments are being conducted to improve the quality of care, but there is insufficient evidence on intensive care units (ICUs). This study aims to evaluate the impact of ICU quality assessments on the structural indicators in medical institutions and the resulting in-hospital mortality of patients.

Methods
This study used data collected in the 2nd and 3rd ICU quality assessments in 2017 and 2019. A total of 72,879 patients admitted to ICUs were included during this period, with 265 institutions that received both assessments. As for structural indicators, changes in medical personnel and equipment were assessed, and in-hospital deaths were evaluated as patient outcomes. To evaluate the association between medical staff and in-hospital mortality, a generalized estimating equation model was performed considering both hospital and patient variables.

Results
Compared to the second quality evaluation, the number of intensivist physicians and experienced nurses increased in the third quality evaluation; however, there was still a gap in the workforce depending on the type of medical institution. Among all ICU patients admitted during the evaluation period, 12.0% of patients died in the hospital. In-hospital mortality decreased at the 3rd assessment, and hospitals employing intensivist physicians were associated with reduced in-hospital deaths. In addition, an increase in the number of experienced nurses was associated with a decrease in in-hospital mortality, while an increase in the nurse-to-bed ratio increased mortality.

Conclusions
ICU quality assessments improved overall structural indicators, but the gap between medical institutions has not improved and interventions are required to bridge this gap. In addition, it is important to maintain skilled medical personnel to bring about better results for patients, and various efforts should be considered. This requires continuous monitoring and further research on long-term effects.
Exercise mimetics: a novel strategy to combat neuroinflammation and Alzheimer’s disease
Authors: Zhao Renqing 
Published: February 2024

AbstractNeuroinflammation is a pathological hallmark of Alzheimer’s disease (AD), characterized by the stimulation of resident immune cells of the brain and the penetration of peripheral immune cells. These inflammatory processes facilitate the deposition of amyloid-beta (Aβ) plaques and the abnormal hyperphosphorylation of tau protein. Managing neuroinflammation to restore immune homeostasis and decrease neuronal damage is a therapeutic approach for AD. One way to achieve this is through exercise, which can improve brain function and protect against neuroinflammation, oxidative stress, and synaptic dysfunction in AD models. The neuroprotective impact of exercise is regulated by various molecular factors that can be activated in the same way as exercise by the administration of their mimetics. Recent evidence has proven some exercise mimetics effective in alleviating neuroinflammation and AD, and, additionally, they are a helpful alternative option for patients who are unable to perform regular physical exercise to manage neurodegenerative disorders. This review focuses on the current state of knowledge on exercise mimetics, including their efficacy, regulatory mechanisms, progress, challenges, limitations, and future guidance for their application in AD therapy.
Action planning for building public health program sustainability: results from a group-randomized trial
Authors: Moreland-Russell Sarah , Combs Todd , Gannon Jessica , Jost Eliot , Farah Saliba Louise , Prewitt Kimberly , Luke Douglas , Brownson Ross C. 
Published: February 2024

Abstract
Background
Public health programs are charged with implementing evidence-based interventions to support public health improvement; however, to achieve long-term population-based benefits, these interventions must be sustained. Empirical evidence suggests that program sustainability can be improved through training and technical assistance, but few resources are available to support public health programs in building capacity for sustainability.

Methods
This study sought to build capacity for sustainability among state tobacco control programs through a multiyear, group-randomized trial that developed, tested, and evaluated a novel Program Sustainability Action Planning Model and Training Curricula. Using Kolb’s experiential learning theory, we developed this action-oriented training model to address the program-related domains proven to impact capacity for sustainability as outlined in the Program Sustainability Framework. We evaluated the intervention using a longitudinal mixed-effects model using Program Sustainability Assessment (PSAT) scores from three time points. The main predictors in our model included group (control vs intervention) and type of dosage (active and passive). Covariates included state-level American Lung Association Score (proxy for tobacco control policy environment) and percent of CDC-recommended funding (proxy for program resources).

Results
Twenty-three of the 24 state tobacco control programs were included in the analyses: 11 received the training intervention and 12 were control. Results of the longitudinal mixed-effects linear regression model, where the annual PSAT score was the outcome, showed that states in the intervention condition reported significantly higher PSAT scores. The effects of CDC-recommended funding and American Lung Association smoke-free scores (proxy for policy environment) were small but statistically significant.

Conclusion
This study found that the Program Sustainability Action Planning Model and Training Curricula was effective in building capacity for sustainability. The training was most beneficial for programs that had made less policy progress than others, implying that tailored training may be most appropriate for programs possibly struggling to make progress. Finally, while funding had a small, statistically significant effect on our model, it virtually made no difference for the average program in our study. This suggests that other factors may be more or equally important as the level of funding a program receives.
Trial registration.
ClinicalTrials.gov, NCT03598114. Registered on July 26, 2018.
Replacement of tibialis cranialis tendon with polyester, silicone-coated artificial tendon preserves biomechanical function in rabbits compared to tendon excision only
Authors: Easton Katrina L. , Hatch Carter , Stephens Kaitlyn , Marler Dylan , Fidelis Obinna , Sun Xiaocun , Bowers Kristin M. , Billings Caroline , Greenacre Cheryl B. , Anderson David E. , Crouch Dustin L. 
Published: February 2024

Abstract
Background
Artificial tendons may be an effective alternative to autologous and allogenic tendon grafts for repairing critically sized tendon defects. The goal of this study was to quantify the in vivo hindlimb biomechanics (ground contact pressure and sagittal-plane motion) during hopping gait of rabbits having a critically sized tendon defect of the tibialis cranialis and either with or without repair using an artificial tendon.

Methods
In five rabbits, the tibialis cranialis tendon of the left hindlimb was surgically replaced with a polyester, silicone-coated artificial tendon (PET-SI); five operated control rabbits underwent complete surgical excision of the biological tibialis cranialis tendon in the left hindlimb with no replacement (TE).

Results
At 8 weeks post-surgery, peak vertical ground contact force in the left hindlimb was statistically significantly less compared to baseline for the TE group (p = 0.0215). Statistical parametric mapping (SPM) analysis showed that, compared to baseline, the knee was significantly more extended during stance at 2 weeks post-surgery and during the swing phase of stride at 2 and 8 weeks post-surgery for the TE group (p < 0.05). Also, the ankle was significantly more plantarflexed during swing at 2 and 8 weeks postoperative for the TE group (p < 0.05). In contrast, there were no significant differences in the SPM analysis among timepoints in the PET-SI group for the knee or ankle.

Conclusions
Our findings suggest that the artificial tibialis cranialis tendon effectively replaced the biomechanical function of the native tendon. Future studies should investigate (1) effects of artificial tendons on other (e.g., neuromuscular) tissues and systems and (2) biomechanical outcomes when there is a delay between tendon injury and artificial tendon implantation.
T-cell infiltration and its regulatory mechanisms in cancers: insights at single-cell resolution
Authors: Yang Wenhui , Liu Shimao , Mao Mengyun , Gong Yandong , Li Xiaohui , Lei Tianyu , Liu Chao , Wu Shikai , Hu Qinyong 
Published: February 2024

AbstractTumor-infiltrating T cells recognize, attack, and clear tumor cells, playing a central role in antitumor immune response. However, certain immune cells can impair this response and help tumor immune escape. Therefore, exploring the factors that influence T-cell infiltration is crucial to understand tumor immunity and improve therapeutic effect of cancer immunotherapy. The use of single-cell RNA sequencing (scRNA-seq) allows the high-resolution analysis of the precise composition of immune cells with different phenotypes and other microenvironmental factors, including non-immune stromal cells and the related molecules in the tumor microenvironment of various cancer types. In this review, we summarized the research progress on T-cell infiltration and the crosstalk of other stromal cells and cytokines during T-cell infiltration using scRNA-seq to provide insights into the mechanisms regulating T-cell infiltration and contribute new perspectives on tumor immunotherapy.
Association of single nucleotide polymorphisms in the F2RL1 gene with clinical and inflammatory characteristics of patients with asthma
Authors: Shrestha Palikhe Nami , Haji Qahir , Mack Emily , Sinnatamby Tristan , Sandford Andrew J. , Cameron Lisa , Vliagoftis Harissios 
Published: February 2024

Abstract
Background
Proteinase-activated receptor 2 (PAR-2) is a G-protein coupled receptor associated with many inflammatory diseases, including asthma. We have shown an association between PAR-2 expression in peripheral blood monocytes and asthma severity as well as blood PAR-2 mRNA level and lung function. Since F2RL1 (the gene encoding PAR-2) polymorphisms affect PAR-2 expression, we hypothesize they may affect asthma severity.

Methods
We recruited 76 subjects with asthma of varying severity and collected clinical (FEV1 [% predicted], FEV1/FVC, IgE) and immunological (PAR-2 mRNA, blood eosinophils) disease parameters. We also genotyped these individuals for 3 F2RL1 SNPs (-45C/T, -149C/G, c.621C/T).

Results
We found that the F2RL1 SNP “C” allele of -45C/T (rs1529505) was associated with PAR-2 mRNA and blood eosinophils. F2RL1 SNP c.621C/T (rs631465) was associated with PAR-2 mRNA. The F2RL1 SNP -149C/G (rs2242991) had no association with any of the parameters studied. This study identified one F2RL1 SNP rs1529505 is associated with parameters of asthma, but not asthma severity.

Conclusion
Larger studies are needed to further elucidate the role of PAR-2 in the pathophysiology of asthma and the influence of genetic variation.
Green tea consumption and incidence of cardiovascular disease in type 2 diabetic patients with overweight/obesity: a community-based cohort study
Authors: Liu Bingyue , Gu Shujun , Zhang Jin , Zhou Hui , Su Jian , Wang Sudan , Sun Qian , Zhou Zhengyuan , Zhou Jinyi , Dong Chen 
Published: February 2024

Abstract
Background
Green tea has been reported to be potentially protective against the development of cardiovascular disease (CVD). This study aimed to investigate the association between green tea consumption and incident CVD in type 2 diabetes (T2D) patients with overweight/obesity.

Methods
A total of 4756 Chinese overweight/obese T2D patients were recruited and followed up for 6.27 years. Information on green tea consumption was collected at baseline using interviewer-administered questionnaires. Hazard ratios (HRs) and 95% confidence intervals (CIs) for incident CVD according to green tea consumption were estimated using the Cox proportional hazards model.

Results
Compared with non-habitual consumers, participants who consumed > 5 g/day of green tea leaves reduced the risk of CVD by 29% (95%CI: 0.55–0.92), stroke by 30% (95%CI: 0.51–0.95) and coronary heart disease (CHD) by 40% (95%CI: 0.40–0.89). Similarly, participants who consumed green tea for ≥ 40 years reduced the risk of CVD by 31% (95%CI: 0.54–0.88), stroke by 33% (95%CI: 0.50–0.90) and CHD by 39% (95%CI: 0.42–0.88). Among participants with < 5-year history of T2D, > 5 g/day of tea leaves and > 40 years of tea consumption were associated with 59% (95%CI: 0.23–0.72) and 57% (95%CI: 0.26–0.74) reduced risk of stroke, respectively. However, among participants with ≥ 5-year history of T2D, > 5 g/day of tea leaves and > 40 years of tea consumption were associated with a 50% (95%CI: 0.30–0.82) and 46% (95%CI: 0.35–0.85) reduced risk of CHD, respectively.

Conclusions
Green tea consumption is associated with reduced risk of CVD, stroke, and CHD in overweight/obese T2D patients.
Body size induced changes in metabolic carbon of soil nematodes under N deposition and precipitation regime change in a temperate grassland
Authors: Cui Shuyan , Mo Xiaomei , Zheng Guo 
Published: February 2024

Abstract
Background
Global climate change has resulted in precipitation regimes exhibiting an increasing trend in rainfall intensity but a reduction in its frequency. Nitrogen (N) deposition is a crucial component of the global N cycling. Nematode body size is a trait that responds to climate change and is used as a standard trait-based indicator in soil community analysis. Variations in body size influence metabolic carbon (C). We examined the ways by which body size and metabolic C of nematodes respond to changing precipitation regimes and how N deposition regulates these responses by an 8-year manipulative experiment.

Methods
Nematode body size was indicated by the community-weighted mean (CWM) mass. We quantified C metabolism components of soil nematodes including production C, respiration C, and corresponding C use efficiency (CUE) under different precipitation intensities and N addition in a semi-arid steppe on the Mongolian Plateau. The Mantel test was used to determine the correlations between CWM, CUE and environmental factors. The partial least squares path modeling (PLS-PM) was conducted to quantify direct or indirect contributions among latent variables.

Results
We found that heavy precipitation intensity increased the CWM mass of total nematodes and omnivores-predators without N addition. N addition decreased CWM mass of bacterivores across all the precipitation intensity treatments. Stronger precipitation intensities might be favorable for nematode production and respiration C. Variations in the nematode CWM mass drove the CUE to change with N addition.

Conclusions
Our findings provide new insights into the mechanisms underlying nematode body size and C metabolism, and highlight that explorative studies, such as manipulative experiments, are needed to identify traits underlying size-related effects and to investigate how they affect CUE of nematodes. These efforts may increase our understanding of how changes in precipitation regimes and N deposition may alter soil nematode communities in grassland ecosystems.
Secrets on the rock: analysis and discussion of the Dunde Bulaq rock art site
Authors: Yihao Fu , Yue Chai , Jun Wang , Cheng Liu , Xiaoyu Zhang , Lu Li , Baheti Zhuoya , Jinye Peng 
Published: February 2024

Abstract
The Altay Prefecture in Xinjiang Uyghur Autonomous Region, China, boasts an abundance source of rock art, with the Dunde Bulaq rock art complex holding paramount significance in the study of local culture. This work employs hyperspectral techniques for data collection and analysis of the Dunde Bulaq I rock art site. Signal decomposition, pigment classification/clustering, and spectral curve analysis were employed to delve deeply into the information of the rock art hyperspectral data. Furthermore, cross-validation was utilized to reveal hidden information within rock art. To comprehend these hidden information, a more profound historical tracing was conducted, employing methods such as literature retrieval and image analysis, uncovering the internal relationship between the site I and the Chemurchek culture. This provides fresh material for studying the Bronze Age cultures within the Altai region.
Remimazolam in perioperative management of Eisenmenger syndrome: a case report
Authors: Hashimoto Kazuya , Matsumoto Tsuguhiro , Mizota Toshiyuki , Kai Shinichi , Egi Moritoki 
Published: February 2024

Abstract
Background
Eisenmenger syndrome (ES) is characterized by severe and irreversible pulmonary hypertension stemming from an uncorrected intracardiac shunt of significant size. The imbalance between systemic and pulmonary artery pressures predisposes patients with ES to the risk of cardiac arrest. Remimazolam has caused less circulatory depression, which may be advantageous for ES. However, no studies reported the use of remimazolam in perioperative ES management.

Case presentation
A 45-year-old female patient with ES derived from a ventricular septal defect was scheduled to undergo bilateral adnexectomy for an ovarian tumor. Her oxygen saturation was 80% with 3 L/min oxygen at rest, and her pulmonary and systemic flow ratio was 0.53. She underwent general anesthesia with remimazolam, and intraoperative hemodynamics was stable without hypotension or reduced oxygen saturation.

Conclusions
Our successful management of ovarian tumor surgery in a patient with ES using remimazolam reveals its potential effectiveness in perioperative care.
Highly dangerous road hazards are not immune from the low prevalence effect
Authors: Song Jiali , Wolfe Benjamin 
Published: February 2024

AbstractThe low prevalence effect (LPE) is a cognitive limitation commonly found in visual search tasks, in which observers miss rare targets. Drivers looking for road hazards are also subject to the LPE. However, not all road hazards are equal; a paper bag floating down the road is much less dangerous than a rampaging moose. Here, we asked whether perceived hazardousness modulated the LPE. To examine this, we took a dataset in which 48 raters assessed the perceived dangerousness of hazards in recorded road videos (Song et al. in Behav Res Methods, 2023. https://doi.org/10.3758/s13428-023-02299-8) and correlated the ratings with data from a hazard detection task using the same stimuli with varying hazard prevalence rates (Kosovicheva et al. in Psychon Bull Rev 30(1):212–223, 2023. https://doi.org/10.3758/s13423-022-02159-0). We found that while hazard detectability increased monotonically with hazardousness ratings, the LPE was comparable across perceived hazardousness levels. Our findings are consistent with the decision criterion account of the LPE, in which target rarity induces a conservative shift in criterion. Importantly, feedback was necessary for a large and consistent LPE; when participants were not given feedback about their accuracy, the most dangerous hazards showed a non-significant LPE. However, eliminating feedback was not enough to induce the opposite of the LPE—prevalence induced concept change (Levari et al. in Science 360(6396):1465–1467, 2018. https://doi.org/10.1126/science.aap8731), in which participants adopt a more liberal criterion when instances of a category become rare. Our results suggest that the road hazard LPE may be somewhat affected by the inherent variability of driving situations, but is still observed for highly dangerous hazards.