bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact on Faculty’s Own Development When Assessing Student Performance
Authors: Barry Erin S , Wang Annie , Cole Rebekah , Yarnell Angela M 
Published: October 2023

ABSTRACT

Introduction
Assessing learner performance is a primary focus within simulation-based education in order to prepare students with the knowledge and skills they will need going forward in their careers. In order to properly conduct these assessments of learner performance, faculty must be adequately trained on the scenario, expectations, assessment measures, and debriefing. During Operation Bushmaster, a five-day “deployment” for learners, faculty assess students as they rotate through different leadership roles. The faculty development includes online and in-person training that provides them with an understanding of the scenario; what learners know; the framework used at USU to guide curriculum, development, and assessment; how to assess learners; and how to provide feedback to learners. Research has examined the value of receiving assessment and feedback from a student perspective, but the impact of being the assessor and giving feedback has not been researched from the faculty point of view. The purpose of this study, therefore, was to examine the impact of assessing students in simulation scenarios on faculty’s own development as an educator and leader.

Materials and Methods
Through a phenomenological qualitative study, we explored participants’ lived experiences as faculty at Operation Bushmaster. Eighteen faculty from a variety of medical specialties and military ranks volunteered to participate. Participants were interviewed in-person using a semi-structured interview. Analyses included individually reading through each transcript; then individually coding and taking notes of terms and phrases used by participants; codes were compiled and organized into categories, which became the themes of our study.

Results
The interviews demonstrated the following themes in which providers who serve as faculty of Operation Bushmaster believe they gain from the experience: (1) The experience helps to reground their own thinking; (2) acting as faculty during simulation-based education helps them remain up-to-date on necessary skills; and (3) working with students helps faculty continually develop as an educator and a leader.

Conclusions
This work describes how even when faculty are brought in for learner assessment, they are taking away lessons and experiences that aid in their own development as an educator as well as a leader. Acting as an assessing faculty for students may allow faculty to reground their own thinking, remain up-to-date on necessary skills, and continually develop their skills as an educator and leader. These findings suggest that some faculty involved in simulation events may also gain knowledge, skills, and experiences that can help with their own development even when the focus is on learners.
Joint analysis constraints on the physics of the first galaxies with low-frequency radio astronomy data
Authors: Bevins Harry T J , Heimersheim Stefan , Abril-Cabezas Irene , Fialkov Anastasia , de Lera Acedo Eloy , Handley William , Singh Saurabh , Barkana Rennan 
Published: October 2023

ABSTRACT
The first billion years of cosmic history remains largely unobserved. We demonstrate, using a novel machine learning technique, how combining upper limits on the spatial fluctuations in the 21-cm signal with observations of the sky-averaged 21-cm signal from neutral hydrogen can improve our understanding of this epoch. By jointly analysing data from the Shaped Antenna measurement of the background RAdio Spectrum (SARAS3, redshift z ≈ 15−25) and limits from the Hydrogen Epoch of Reionization Array (HERA, z ≈ 8 and 10), we show that such a synergetic analysis provides tighter constraints on the astrophysics of galaxies 200 million years after the big bang than can be achieved with the individual data sets. Although our constraints are weak, this is the first time data from a sky-averaged 21-cm experiment and power spectrum experiment have been analysed together. In synergy, the two experiments leave only $64.9^{+0.3}_{-0.1}$ per cent of the explored broad theoretical parameter space to be consistent with the joint data set, in comparison to $92.3^{+0.3}_{-0.1}$ per cent for SARAS3 and $79.0^{+0.5}_{-0.2}$ per cent for HERA alone. We use the joint analysis to constrain star formation efficiency, minimum halo mass for star formation, X-ray luminosity of early emitters, and the radio luminosity of early galaxies. The joint analysis disfavours at 68 per cent confidence a combination of galaxies with X-ray emission that is ≲33 and radio emission that is ≳32 times as efficient as present day galaxies. We disfavour at 95 per cent confidence scenarios in which power spectra are ≥126 mK2 at z = 25 and the sky-averaged signals are ≤−277 mK.
T-Cell Exhaustion in HIV-1/Hepatitis C Virus Coinfection Is Reduced After Successful Treatment of Chronic Hepatitis C
Authors: Caraballo Cortés Kamila , Osuch Sylwia , Perlejewski Karol , Radkowski Marek , Janiak Maciej , Berak Hanna , Rauch Andri , Fehr Jan S , Hoffmann Matthias , Günthard Huldrych F , Metzner Karin J 
Published: October 2023

Abstract

Background
T-cell responses during chronic viral infections become exhausted, which is reflected by upregulation of inhibitory receptors (iRs) and increased interleukin 10 (IL-10). We assessed 2 iRs—PD-1 (programmed cell death protein 1) and Tim-3 (T-cell immunoglobulin and mucin domain–containing protein 3)—and IL-10 mRNAs in peripheral blood mononuclear cells (PBMCs) and their soluble analogs (sPD-1, sTim-3, and IL-10) in plasma in chronic HIV-1/hepatitis C virus (HCV) coinfection and explored the effect of HCV treatment on these markers. We also aimed to establish whether iR expression may be determined by the HCV CD8+ T-cell immunodominant epitope sequence.

Methods
Plasma and PBMCs from 31 persons with chronic HIV-1/HCV coinfection from the Swiss HIV Cohort Study were collected before and after HCV treatment. As controls, 45 persons who were HIV-1 negative with chronic HCV infection were recruited. Exhaustion markers were assessed by enzyme-linked immunosorbent assay in plasma and by quantitative reverse transcription polymerase chain reaction in PBMCs. Analysis of an HCV epitope sequence was conducted by next-generation sequencing: HLA-A*02–restricted NS31073–1081 and NS31406–1415 and HLA-A*01–restricted NS31436–1444.

Results
The study revealed higher plasma sPD-1 (P = .0235) and IL-10 (P = .002) levels and higher IL-10 mRNA in PBMCs (P = .0149) in HIV-1/HCV coinfection. A decrease in plasma sPD-1 (P = .0006), sTim-3 (P = .0136), and IL-10 (P = .0003) and Tim-3 mRNA in PBMCs (P = .0210) was observed following successful HCV treatment. Infection with the HLA-A*01–restricted NS31436–1444 ATDALMTGY prototype variant was related to higher sTim-3 levels than infection with the ATDALMTGF escape variant (P = .0326).

Conclusions
The results underscore the synergistic effect of coinfection on expression of exhaustion markers, their reduction following successful HCV treatment and imply that iR levels may operate on an epitope-specific manner.
Incidence and Outcomes of Bloodstream Infection After Arterial Aneurysm Repair: Findings From a Population-Based Study
Authors: Tabaja Hussam , Baddour Larry M , Chesdachai Supavit , DeMartino Randall R , Lahr Brian D , DeSimone Daniel C 
Published: October 2023

Abstract

Background
Limited research has focused on bloodstream infection (BSI) in patients with arterial grafts. This study aims to describe the incidence and outcomes of BSI after arterial aneurysm repair in a population-based cohort.

Methods
The expanded Rochester Epidemiology Project (e-REP) was used to analyze aneurysm repairs in adults (aged ≥18 years) residing in 8 counties in southern Minnesota from January 2010 to December 2020. Electronic records were reviewed for the first episode of BSI following aneurysm repair. BSI patients were assessed for vascular graft infection (VGI) and followed for all-cause mortality.

Results
During the study, 643 patients had 706 aneurysm repairs: 416 endovascular repairs (EVARs) and 290 open surgical repairs (OSRs). Forty-two patients developed BSI during follow-up. The 5-year cumulative incidence of BSI was 4.7% (95% confidence interval [CI], 3.0%–6.4%), with rates of 4.0% (95% CI, 1.8%–6.2%) in the EVAR group and 5.8% (95% CI, 2.9%–8.6%) in the OSR group (P = .052). Thirty-nine (92.9%) BSI cases were monomicrobial, 33 of which were evaluated for VGI. VGI was diagnosed in 30.3% (10/33), accounting for 50.0% (8/16) of gram-positive BSI cases compared to 11.8% (2/17) of gram-negative BSI cases (P = .017). The 1-, 3-, and 5-year cumulative post-BSI all-cause mortality rates were 22.2% (95% CI, 8.3%–34.0%), 55.8% (95% CI, 32.1%–71.2%), and 76.8% (95% CI, 44.3%–90.3%), respectively.

Conclusions
The incidence of BSI following aneurysm repair was overall low. VGI was more common with gram-positive compared to gram-negative BSI. All-cause mortality following BSI was high, which may be attributed to advanced age and significant comorbidities in our cohort.
miR-181b-5p/SOCS2/JAK2/STAT5 axis facilitates the metastasis of hepatoblastoma
Authors: Lv Yong , Xie Xiaolong , Zou Guoyou , Kong Meng , Yang Jiayin , Chen Jing , Xiang Bo 
Published: October 2023

Abstract

Introduction
Hepatoblastoma (HB) is a malignant liver tumor predominantly found in children and tumor metastasis is one of the main causes of poor prognosis in affected patients. The precise molecular mechanisms responsible for HB metastasis remain incompletely understood. However, there is evidence suggesting a connection between the dysregulation of microRNAs (miRNAs) and the progression of tumor metastasis in HB.

Methods
The study utilized weighted gene co-expression network analysis (WGCNA) to analyze a miRNA microarray dataset of HB. The expression of miR-181b-5p in HB tissues and cells was detected using quantitative real-time PCR. The impact of miR-181b-5p on the metastatic capacity of HB was evaluated through scratch and Transwell assays. The effects of exogenously expressing miR-181b on the metastatic phenotypes of HB cells were evaluated in vivo. Furthermore, a luciferase reporter assay was performed to validate a potential target of miR-181b-5p in HB.

Results
We found that miR-181b-5p was highly expressed in HB tissues and HB cell lines. Overexpression of miR-181b enhanced scratch healing, cell migration, and invasion abilities in vitro, as well as enhancing HB lung metastasis potential in vivo. Dual-luciferase reporter assays showed that Suppressor Of Cytokine Signaling 2 (SOCS2) was a direct target of miR-181b. The overexpression of miR-181b resulted in the suppression of SOCS2 expression, subsequently activating the epithelial–mesenchymal transition and JAK2/STAT5 signaling pathways. The rescue experiment showed that SOCS2 overexpression attenuated the effects of miR-181b on HB cells.

Conclusion
Our study showed that miR-181b promotes HB metastasis by targeting SOCS2 and may be a potential therapeutic target for HB.
Sterol Biosynthesis Contributes to Brefeldin-A-Induced Endoplasmic Reticulum Stress Resistance in Chlamydomonas reinhardtii
Authors: Je Sujeong , Choi Bae Young , Kim Eunbi , Kim Kyungyoon , Lee Yuree , Yamaoka Yasuyo 
Published: October 2023

Abstract
The endoplasmic reticulum (ER) stress response is an evolutionarily conserved mechanism in most eukaryotes. In this response, sterols in the phospholipid bilayer play a crucial role in controlling membrane fluidity and homeostasis. Despite the significance of both the ER stress response and sterols in maintaining ER homeostasis, their relationship remains poorly explored. Our investigation focused on Chlamydomonas strain CC-4533 and revealed that free sterol biosynthesis increased in response to ER stress, except in mutants of the ER stress sensor Inositol-requiring enzyme 1 (IRE1). Transcript analysis of Chlamydomonas experiencing ER stress unveiled the regulatory role of the IRE1/basic leucine zipper 1 pathway in inducing the expression of ERG5, which encodes C-22 sterol desaturase. Through the isolation of three erg5 mutant alleles, we observed a defect in the synthesis of Chlamydomonas’ sterol end products, ergosterol and 7-dehydroporiferasterol. Furthermore, these erg5 mutants also exhibited increased sensitivity to ER stress induced by brefeldin A (BFA, an inhibitor of ER–Golgi trafficking), whereas tunicamycin (an inhibitor of N-glycosylation) and dithiothreitol (an inhibitor of disulfide-bond formation) had no such effect. Intriguingly, the sterol biosynthesis inhibitors fenpropimorph and fenhexamid, which impede steps upstream of the ERG5 enzyme in sterol biosynthesis, rescued BFA hypersensitivity in CC-4533 cells. Collectively, our findings support the conclusion that the accumulation of intermediates in the sterol biosynthetic pathway influences ER stress in a complex manner. This study highlights the significance and complexity of regulating sterol biosynthesis during the ER stress response in microalgae.
Bursts of coalescence within population pedigrees whenever big families occur
Authors: Diamantidis Dimitrios , Fan Wai-Tong (Louis) , Birkner Matthias , Wakeley John 
Published: October 2023

AbstractWe consider a simple diploid population-genetic model with potentially high variability of offspring numbers among individuals. Specifically, against a backdrop of Wright-Fisher reproduction and no selection there is an additional probability that a big family occurs, meaning that a pair of individuals has a number of offspring on the order of the population size. We study how the pedigree of the population generated under this model affects the ancestral genetic process of a sample of size two at a single autosomal locus without recombination. Our population model is of the type for which multiple-mergers coalescent processes have been described. We prove that the conditional distribution of the pairwise coalescence time given the random pedigree converges to a limit law as the population size tends to infinity. This limit law may or may not be the usual exponential distribution of the Kingman coalescent, depending on the frequency of big families. But because it includes the number and times of big families it differs from the usual multiple-merger coalescent models. The usual multiple-merger coalescent models are seen as describing the ancestral process marginal to, or averaging over, the pedigree. In the limiting ancestral process conditional on the pedigree, the intervals between big families can be modeled using the Kingman coalescent but each big family causes a discrete jump in the probability of coalescence. Analogous results should hold for larger samples and other population models. We illustrate these results with simulations and additional analysis, highlighting their implications for inference and understanding of multi-locus data.
Theranostic multifunctional lipid nanoparticles containing curcumin for integrated imaging and stabilizing vulnerable atherosclerotic plaques through an “eat-me” signal
Authors: Shi Zhang , Huang Jun , Chen Chao , Zhang Xuefeng , Ma Zhiqiang , Liu Qi 
Published: October 2023

ABSTRACTBACKGROUNDCurcumin has emerged as a promising candidate capable of polarization of macrophages, which promote the stability of atherosclerotic plaque. Nevertheless, a notable limitation lies in the non-specific nature of curcumin’s targeting. The present study endeavors to harness multifunctional lipid nanoparticles (MLNPs), which could both facilitate imaging and achieve targeted delivery of curcumin specifically to inflammatory macrophages, to effectively counteract vulnerable plaque and mitigate the risk of ischemic events.METHODSThe term “MLNPs”, for targeted delivery of curcumin using multimodal imaging techniques including single photon emission computed tomography (SPECT) and magnetic resonance imaging (MRI), refers to a new type of nanoparticle designed to specifically target and modulate macrophages with phagocytic function. These nanoparticles are cholesteryl-9-carboxynonanoate-(125I-iron oxide nanoparticle/Cur)-lipid-coated nanoparticles [9-CCN-(125I-ION/Cur)-LNPs], which carry hybrid imaging agents. These agents are combinations of 125I-ION and lipids that contain phagocytic “eat-me” signals, which induces macrophages to swallow MLNPs.RESULTSThe accumulation of the devised 9-CCN-(125I-ION/Cur)-LNPs on the unstable plaque of animal models in vivo was accurately reflected and lesions were highlighted by both SPECT and MRI. The intense radioactive signals on SPECT images facilitated the identification and quantification of the target lesion, while MRI based on ION particles facilitated the visualization of the focal localization and volumetry of atherosclerotic plaque. The targeted distribution of the unstable plaque in the rabbit aorta was further confirmed by ex vivo planar images of nuclide and Prussian blue staining for ION. Additionally, 9-CCN-(125I-ION/Cur)-LNPs were found to specifically and effectively bind to the pro-inflammatory M1 macrophages present in the unstable plaque, resulting in the accumulation of radionuclide and hypointensity on T2W images.CONCLUSIONSThe 9-CCN-(125I-ION/Cur)-LNPs demonstrated remarkable capability in the delivery of both125I-ION and curcumin to macrophages, ultimately resulting in M1-M2 macrophage polarization, which may offer valuable insights into addressing unstable atherosclerotic plaque.
Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in early mouse embryos
Authors: Hou Yanlin , Nie Zhengwen , Jiang Qi , Velychko Sergiy , Heising Sandra , Bedzhov Ivan , Wu Guangming , Adachi Kenjiro , Schöler Hans R. 
Published: October 2023

AbstractDuring the first lineage segregation, mammalian embryos generate the inner cell mass (ICM) and trophectoderm (TE). ICM gives rise to the epiblast (EPI) that forms all cell types of the body, an ability referred to as pluripotency. The molecular mechanisms that induce pluripotency in embryos remain incompletely elucidated. Using knockout (KO) mouse models in conjunction with low-input ATAC-seq and RNA-seq, we found that Oct4 and Sox2 gradually come into play in the early ICM, coinciding with the initiation of Sox2 expression. Oct4 and Sox2 activate the pluripotency-related genes through the putative OCT-SOX enhancers in the early ICM. Furthermore, we observed a substantial reorganization of chromatin landscape and transcriptome from the morula to the early ICM stages, which was partially driven by Oct4 and Sox2, highlighting their pivotal role in promoting the developmental trajectory towards the ICM. Our study provides new insights into the establishment of the pluripotency network in mouse preimplantation embryos.
Identification and analysis of genomic regions influencing leaf morpho-physiological traits related to stress responses inDioscorea alata
Authors: Dossa Komivi , Houngbo Mahugnon Ezékiel , Irep Jean-Luc , Chair Hâna , Cornet Denis 
Published: October 2023

AbstractBackgroundYams (Dioscoreaspp.) are significant food security crops especially in West Africa. With the increasing tuber demand and climate change challenges, it is pertinent to strengthen breeding programs for developing high-yielding cultivars with climate resilience. The current study aimed at deciphering the genetic basis of leaf traits related to stress responses in a diverse panel ofDioscorea alatagenotypes.ResultsPhenotypic characterization of 12 traits, including leaf dry matter content, leaf area, net photosynthesis, transpiration rate, transpiration use efficiency, stomatal density, stomatal index, node number, leaf thickness, competitor, stress-tolerator, ruderal (CSR) ecological strategy spectrum emphasized significant variations among the genotypes and across two planting locations. Weak correlations were observed among most of traits, suggesting that breeding simultaneously for some of these stress response-related traits would be possible. Heritability was highest for transpiration rate, leaf area and stomatal density, while it was lowest for stress-tolerator, ruderal ecological strategies. Genome-wide association study (GWAS) using high-quality single nucleotide polymorphism (SNPs) identified 24 significant associations on 11 chromosomes, where the association signals were consistent across two locations for traits with high heritability, viz., stomatal density (Chr18) and transpiration rate (Chr3). Further characterization of the significant signals and their related alleles identified advantageous alleles contributing positively to the studied traits. Moreover, 44 putative candidate genes were identified.Dioal.18G049300(3keto acyl-coenzyme A synthase)was identified as a strong candidate gene for stomatal density, whileDioal.12G033600(Phosphatidyl inositol monophosphate 5 kinase 4) was identified for net photosynthesis.ConclusionTaken together, GWAS and allele segregation analysis for key SNPs provided significant insights into the marker-trait associations, which can be further utilized in breeding programs to improve climate resilience in greater yam.
Foot orientation and trajectory variability in locomotion: effects of real-world terrain
Authors: Gibson Emma , Douglas Greg , Jeffries Katelyn , Delaurier Julianne , Chestnut Taylor , Charlton Jesse M. 
Published: October 2023

AbstractCapturing human locomotion in nearly any environment or context is becoming increasingly feasible with wearable sensors, giving access to commonly encountered walking conditions. While important in expanding our understanding of locomotor biomechanics, these more variable environments present challenges to identify changes in data due to person-level factors among the varying environment-level factors. Our study examined foot-specific biomechanics while walking on terrain commonly encountered with the goal of understanding the extent to which these variables change due to terrain. We recruited healthy adults to walk at self-selected speeds on stairs, flat ground, and both shallow and steep sloped terrain. A pair of inertial measurement units were embedded in both shoes to capture foot biomechanics while walking. Foot orientation was calculated using a strapdown procedure and foot trajectory was determined by double integrating the linear acceleration. Stance time, swing time, cadence, sagittal and frontal orientations, stride length and width were extracted as discrete variables. These data were compared within-participant and across terrain conditions. The physical constraints of the stairs resulted in shorter stride lengths, less time spent in swing, toe-first foot contact, and higher variability during stair ascent specifically (p<0.05). Stride lengths increased when ascending compared to descending slopes, and the sagittal foot angle at initial contact was greatest in the steep slope descent condition (p<0.05). No differences were found between conditions for horizontal foot angle in midstance (p≥0.067). Our results show that walking on slopes creates differential changes in foot biomechanics depending on whether one is descending or ascending, and stairs require different biomechanics and gait timing than slopes or flat ground. This may be an important factor to consider when making comparisons of real-world walking bouts, as greater proportions of one terrain feature in a data set could create bias in the outcomes. Classifying terrain in unsupervised walking datasets would be helpful to avoid comparing metrics from different walking terrain scenarios.