bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of State and Trait Anxiety on Pain-Pressure Threshold
Authors: Lowe Scott , Downing Katelyn , Fair Breana , Hughey Christian 
Published: January 2024

Abstract
Background:
The global burden of persistent pain is substantial, affecting up to 30% of the world's population. Despite growing awareness, challenges persist with accurate pain measurement, limiting research and clinical efforts. One method to assess pain perception is the Pain-Pressure Threshold (PPT), indicating the pressure at which pain is reported. Anxiety, comprising state and trait anxiety, has been suggested as a potential influencer of PPT, yet its precise impact remains uncertain. State anxiety is defined as a "transient state of arousal subjectively experienced as anxiety" Trait anxiety is “an individual's tendency to appraise situations as threatening, avoid anxiety-provoking situations, and demonstrate high baseline physiological arousal”
Methods:
Thirty-eight participants with no history of persistent pain or anxiety completed data collection, 19 men and 19 women, ages 22-32. Subjects completed the State-Trait Anxiety Inventory-5 (STAI-5) and underwent PPT testing at the upper trapezius and forearm extensors bilaterally, with results averaged to provide an extrapolation of global pressure tolerance. The same data was collected again 21 days later.
Results:
No correlation existed between anxiety and PPT. No significant correlation (p=.148) was observed in State anxiety scores between baseline and follow-up, while trait anxiety displayed a moderate relationship (r=.612, p<.001) between the initial and subsequent tests. These findings support the notion that state anxiety is more variable, contrasting with the presumed stability of trait anxiety. Pain-pressure threshold test-retest data revealed a moderate relationship (r=.542, p<.001), consistent with prior research on PPT test reliability.
Discussion/Conclusion:
This research is unique in that we assessed the relationship between anxiety and PPT in young, healthy participants. The principal finding is that pain-pressure thresholds were not shown to be influenced by either trait or state anxiety in our sample. The data also provided further information about the test-retest consistency of the STAI-5 and PPT measures. This research provides initial baseline data, against which findings in other populations can be compared. Future research should continue to aim to quantify psychosocial factors and their influence on pain experiences.
Trial Registration: This study was registered on 21/09/2023 on ClinicalTrials.gov: # NCT06047704
Glutamine Deprivation Suppresses Lung Cancer Metastasis via Inducing the Activation of ZAKα and Translation Inhibition
Authors: Lei Yinrui , Lin Huiyun , Liu Peiyan , Guo Fang 
Published: January 2024

Abstract
Background
Amino acids are not only components of proteins, but also play important roles in many cellular processes, such as regulating the mTORC1 activity, controlling the translation. However, the role of each amino acid on lung cancer metastasis is still uncertain.
Methods
Transwell assays were used to detect to migration and invasion of cancer cells. Real Time PCR was used to detect the transcription of genes. RNA-seq was performed to analyze the signature of EMT-related genes. Western blot was used to detect the level of proteins. Phospho-gel was used to detect the activation of ZAKα. Puromycin incorporation assay was conducted to show the extent of translation. Lentivirus packaging was used to construct stable cell lines. Mutagenesis was conducted to mimic the dominant-negative or activation of proteins. Mouse lung seeding was used to detect the metastasis of tumor cells in vivo.
Results
Here, we found that, among the 20 basic amino acids, glutamine deprivation specifically inhibits EMT, migration and invasion of lung cancer cells. While, this effect of glutamine is independent of its metabolism. Mechanistically, we found that glutamine promotes A549 cell migration and invasion by enhancing the translation of Zeb1 and Snail. The phosphorylation of translation factors: eIF2α S51, eIF4E S209 and eEF2 S57 induced by glutamine limitation is dispensable for the translation of Zeb1 and Snail. Further, glutamine deprivation dramatically activates ZAKα and its substrates JNK and p38. Knockdown of ZAKα rescues lung cancer cell migration and invasion under glutamine deprivation, while constitutive activation of ZAKα W347S or F368C inhibits lung cancer cell migration and invasion in the presence of glutamine. In mouse models, ZAKα W347S or F368C inhibits the lung seeding of A549 cells.
Conclusions
Glutamine depletion could activate ZAKα to inhibit the translation of EMT-TFs and thus suppress the lung cancer cell metastasis. Our findings uncover the special effect of glutamine on the regulation of translation and lung cancer metastasis, which may give us a new insight into nutrient and cell fate during lung cancer development.
Polyaniline/SiO2 Nanocomposite Catalyzed One-pot Three Component Synthesis of 3,4-dihydropyrimidin-2(1H)-one Derivatives
Authors: Yelwande Ajeet A. , Navgire Madhukar E. , Tayde Deepak T. , Lande Machhindra K. 
Published: January 2024

Abstract
An efficient and rapid protocol for the synthesis of one-pot 3,4-dihydropyrimidin-2(1H)-ones has been developed by the polyaniline/SiO2 catalyzed condensation of benzaldehydes, β-ketoesters and urea/ thiourea. Polyaniline/SiO2 nanocomposite was synthesized by chemical oxidative polymerization of aniline monomer on the surface of SiO2 in the presence potassium persulphate (K2S2O8) as oxidizing agent and sulfuric acid (H2SO4).The synthesized catalytic materials have been well characterized by means of transmission electron microscopy (TEM), thermal analysis (TG-DTA), X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), Temperature programmed desorption (NH3-TPD) and Brunauer Emmett and Teller (BET) method. Solvent stability of catalyst was tested using UV-Visible spectroscopy. Thermal stability of Polyaniline is noticeably improved which may due to the insertion of polyaniline on the surface of SiO2. NH3-TPD was used to determine the acidic properties of solid catalyst Polyaniline/SiO2 indicate the weak, medium and strong acidic sites total acidic strength is found to be 0.2906 mmol/gm. The specific surface area and average pore diameter of 10 wt % polyaniline/SiO2 are found 75.3443 m²/g and 10.8823 nm. This protocol has several advantages such as good thermal stability, high yield, simple work up procedure, non-toxic, clean, and easy recovery and reusability of the catalyst system.
Investigation of physio-mechanical, antioxidant and antimicrobial properties of starch- zinc oxide nanoparticles active films reinforced with Ferula gummosa Boiss essential oil
Authors: Sarhadi Hamid , Shahdadi Fatemeh , Sardoei Ali Salehi , Hatami Mehrnaz , Ghorbanpour Mansour 
Published: January 2024

Abstract
The production of surface compounds coated with active substances has attracted a lot of attention in recent years. In this study, the physical, mechanical, antioxidant, and antimicrobial properties of starch/zinc oxide nanoparticles (ZnO NPs) composite containing various concentrations of Ferula gummosa Boiss essential oil (0.5, 1 and 1.5%) were investigated. The addition of ZnO NPs improved the thickness, and the mechanical and microbial properties, and on the other hand, reduced the water vapor permeability of the starch active film. The addition of Ferula gummosa Boiss essential oil to starch nanocomposite decreased the water vapor permeability from 6.25 to 5.63 g mm− 2 d− 1 kPa− 1, but this decrease was significant only in the concentration of 1.5% of essential oils (p < 0.05). The addition of essential oil to starch nanocomposite caused a decrease in Tensile Strength (TS) value and an increase in Elongation at Break (EB) values was obtained by adding 1.5% of Ferula gummosa Boiss essential oil to starch. The results of the antimicrobial activity of the nanocomposite showed that the pure starch film did not show any lack of growth zone. The addition of ZnO NPs to the starch matrix caused antimicrobial activity on both studied bacteria (Staphylococcus aureus, Escherichia coli). The highest antimicrobial activity was observed in the starch/ ZnO NPs film containing 1.5% essential oil with an inhibition zone of 340 mm2 on Staphylococcus aureus. Antioxidant activity increased significantly with increasing concentration of Ferula gummosa Boiss essential oil (P < 0.05). The film containing 1.5% essential oil had the highest (50.5%) antioxidant activity. In this study, the effect of starch/ ZnO NPs composite containing Ferula gummosa Boiss essential oil was also investigated in order to increase the shelf life of rainbow salmon fillets. Microbial load, lactic acid bacteria and psychrophilic bacteria of salmon fillets were reduced by coating. Coating also improved the chemical characteristics of fish fillet. In general, the starch nanocomposite containing ZnO NPs and Ferula gummosa Boiss essential oil has the ability to be used in the aquatic packaging industry.
Morbidity and neurodevelopmental outcomes at 2 years in preterm infants undergoing percutaneous transcatheter closure vs. surgical ligation of the PDA
Authors: Ramos Maria Fernandez , Reichlin Amy , Kase Jordan , Giamelli Joseph 
Published: January 2024

Abstract

Objective: Review a cohort of preterm infants <29 weeks of gestation at birth and compare morbidities and neurodevelopmental outcomes based on type of PDA closure.
Study design: Single center observational retrospective-prospective case control study of premature infants who underwent surgical ligation or percutaneous transcatheter closure of the PDA. Neurodevelopmental testing was done using the Bayley Scales of Infant Development 3rd ed.
Results: The percutaneous transcatheter closure group had an older corrected gestational age and weight at the time of procedure, and started enteral feeds and achieved room air status at an earlier post procedure day. Infants in the surgical ligation group were more likely to experience vocal cord paralysis. There was no difference in neurodevelopmental outcomes between groups.
Conclusion: Waiting for infants to achieve the appropriate size for percutaneous transcatheter closure of the PDA may lead to reduced short-term complications without increasing the risk of neurodevelopmental impairment.
Comparison of the Myopia Control Effectiveness and Defocus of OK and HAL in Adolescents
Authors: Yu Jia , Guo Yujuan , Zhou Yuehua 
Published: January 2024

Abstract
Purpose
To compare the control effectiveness and periretinal defocus between orthokeratology(OK) and highly aspherical lenslets(HAL) in adolescents with myopia.
Methods
In this prospective study, 211 children( 211 eyes) were enrolled in Beijing Ming Vision and Ophthalmology between October 2022 and February 2023. According to the myopic correction method, the children were divided into three subgroups: OK, HAL, and Single-Vision spectacles (SVS); according to the spherical equivalent (SE), they were further divided into two subgroups: A-1.0 ≤ SE≤-2.0D, B-2.0<SE≤-3.0 D.All children were followed up for12mo,and the changes in axial length and periretinal defocus before and after treatment were recorded and the factors affecting the corneal shaping force for one day post-OK were analyzed.
Results
AL growth in the OK and HAL was lower than that in the SVS after 6,9,12mo. Comparison of the difference in the amount of AL change between OK and HAL: 6mo( Z=-1.367 ,P = 0.317) ; 9mo(Z=-1.45, P = 0.211) ;12mo( Z=-1.52, P = 0.365 ), the difference was not statistically significant with (-1.0 ≤ SE≤-2.0D). The myopic defocus of RDV270-300° in the HAL subgroup was higher than that in the OK subgroup(Z = 1.382,P = 0.016),and in other areas,the myopic defocus was higher in the OK subgroup. Em,SRI ,and corneal thickness were significantly associated with shaping force.
Conclusions
OK is one of the most effective optical treatments for controlling myopia and the factors that affect the corneal shaping force under different corneal parameters are different.For mild myopia, HAL can achieve the same effectiveness as OK.
A Deep Dive into GI Tract Imaging Transformation through Advanced Image Segmentation Analysis with Deep Learning
Authors: Vidyullatha Pellakuri , Tirumala Sreeram , PVL Madhav , Sai Pavan , Vivek Manda DVSSRK , Ghantasala G S Pradeep , Allabun Sarah , ELSHIEKH E. , Othman Manal , Abbas Mohamed , Soufiene Ben Othman 
Published: January 2024

Abstract
The reconstruction of computed gastrointestinal tract tomography images has been a vibrant field of study, particularly with the emergence of deep learning techniques. These methods leverage data-driven models to enhance the quality of reconstructions. Our research delves into this domain by conducting a comprehensive data challenge, where various deep learning algorithms were assessed using extensive public datasets. The focal point was on quantitatively evaluating these methods. A noticeable outcome of our investigation is the substantial enhancement in reconstruction quality metrics achieved by deep learning-based approaches, both in applications involving computed tomography (CT) and using methods such as Region-CNN (RCNN) and Conditional Invertible Neural Networks (CINN). We also delve into crucial selection criteria for these methods, encompassing factors like the availability of training data, understanding the physical measurement model, and the speed of reconstruction. The prevailing technique for segmenting three-dimensional tract images relies on convolutional networks and Conditional Invertible Neural Networks. Yet, these advanced architectures, including CNN, RNN, and CINN, impose heavy computational demands, necessitating GPU-accelerated workstations for rapid inference. This research work introduces a novel segmentation method employing a human-like strategy for 3D segmentation where initially analyzes the image at a small scale to pinpoint areas of interest, subsequently processing only pertinent feature-map patches. This innovation drastically reduces inference time and all while upholding state-of-the-art segmentation quality.
Key gene modules and hub genes associated with pyrethroid and organophosphate resistance in Anopheles mosquitoes: A systems biology approach
Authors: Odhiambo Cynthia Awuor , Derilus Dieunel , Impoinvil Lucy Mackenzie , Omoke Diana , Saizonou Helga , Okeyo Stephen , Dada Nsa , Mulder Nicola , Nyamai Dorothy , Nyanjom Steven , Lenhart Audrey , Djogbénou Luc , Ochomo Eric 
Published: January 2024

Abstract
Indoor residual spraying (IRS) and insecticide-treated nets (ITNs) are the main methods used to control mosquito populations for malaria prevention. The efficacy of these strategies is threatened by the spread of insecticide resistance (IR), limiting the success of malaria control. Studies of the genetic evolution leading to insecticide resistance could enable the identification of molecular markers that can be used for IR surveillance and an improved understanding of the molecular mechanisms associated with IR. This study used a weighted gene co-expression network analysis (WGCNA) algorithm, a systems biology approach, to identify genes with similar co-expression patterns (modules) and hub genes that are potential molecular markers for insecticide resistance surveillance in Kenya and Benin. A total of 20 and 26 gene co-expression modules were identified via the average linkage hierarchical clustering from Anopheles arabiensis and An. gambiae, respectively, and hub genes (highly connected genes) identified within each module. Four specific genes stood out: serine protease, E3 ubiquitin-protein ligase, cuticular protein RR2, and leucine-rich immune protein, which were top hub genes in both species and could serve as potential markers and targets for monitoring IR in these malaria vectors. In addition to the identified markers, we explored molecular mechanisms using enrichment maps that revealed a complex process involving multiple steps, from odorant binding and neuronal signaling to cellular responses, immune modulation, cellular metabolism, and gene regulation. Incorporation of these dynamics into the development of new insecticides and tracking of insecticide resistance could improve sustainable and cost-effective deployment of interventions.
The impact of college enrollment expansion policy on income gap: a case study from China
Authors: HUANG Yutong , DUAN Jinghui 
Published: January 2024

Abstract
This study focuses on the empirical research on the impact of college enrollment expansion policy on the income gap of residents. In this paper, the distribution characteristics of the sample survey data of residents’ income before and after the college enrollment expansion policy (1989–2015) in China Health and Nutrition Survey Database (CHNS) are firstly investigated. And the counterfactual analysis of the income gap of residents before and after the college enrollment expansion policy is conducted by using Recentered Influence Function (RIF) regression modeling and Firpo Fortin Lemieux (2009) (FFL) decomposition method respectively. The results show that: (1) The income gap of residents before and after the college enrollment expansion policy is the result of the combined effect of the “effect of returns to education” and the “effect of labor structure”. The “effect of returns to education” widens the income gap, while the “effect of labor structure” narrows the income gap; (2) The returns to education of urban residents under the college enrollment expansion policy is significantly higher than that of rural residents, resulting in the widening income gap among residents; (3) There is gender discrimination in the labor market. Women’s education opportunities have increased after the college enrollment expansion policy, however, the returns to education of female residents is still lower than that of male residents. Based on the empirical test results, relevant policies and suggestions are proposed from the perspective of educational development.
Prognostic Factors Impacting Post-Transplant Outcomes in Adult T-cell Acute Lymphoblastic Leukemia: A Registry-Based Study by the EBMT Acute Leukemia Working Party
Authors: Cheikh Jean El , Ngoya Maud , Galimard Jacques-Emmanuel , Remenyi P , Kulagin Alexander , Aljurf Mahmoud D , Bahar Babak , Wu Depei , Arat Mutlu , Salmenniemi Urpu , CASTILLA-LLORENTE Cristina , Socie Gerard , Helbig Grzegorz , schroeder thomas , Sakellari Ioanna , Rambaldi Alessandro , Carpenter Benjamin , labussiere helene , Stelljes Matthias , Brissot Eolia , giebel s , Peric Zinaida , Nagler Arnon , Ciceri Fabio , Bazarbachi Ali , Mohty Mohamad , Busca Alessandro 
Published: January 2024

Abstract

Background
T-cell acute lymphoblastic leukemia (T-ALL) predominantly affects individuals in late childhood and young adulthood. Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a curative modality particularly in the setting of poor risk genetics and/or persistent minimal residual disease. Limited studies have directly explored the impact of patient- and transplant-related factors on post-transplant outcomes in T-ALL.
Methods
Using a large dataset from the European Society for Blood and Marrow Transplantation registry, we identified 1907 adult T-ALL patients (70% male) who underwent their first allo-HSCT in first complete remission (CR1) from matched sibling donors (MSD; 45%), unrelated donors (UD; 43%) or haploidentical donors (12%) between 2010 and 2021.
Results
The median age at transplant was 33.4 years (18.1–75). The median follow up was 2.9 years. Most patients underwent total body irradiation (TBI)-based myeloablative conditioning (69%). The 2-year overall survival (OS) was 69.4%, and leukemia -free survival (LFS) was 62.1%. In multivariate analysis, advanced age at transplant negatively affected LFS (for each 10-year increment, HR = 1.11, p = 0.004), GVHD-free, relapse-free survival (GRFS) (HR = 1.06, p = 0.04), OS (HR = 1.12, p = 0.002), and non-relapse mortality (NRM) (HR = 1.23, p < 0.001). A later year of HSCT was associated with improved GFRS (For each 3-year increment, HR = 0.89, p < 0.001), OS (HR = 0.9, p = 0.02), and decreased NRM (HR = 0.82, p = 0.008). TBI improved LFS (HR = 0.79, p = 0.02), GRFS (HR = 0.83, p = 0.04), and relapse incidence (RI) (HR = 0.65, p < 0.001). Female-to-male transplant negatively affected GRFS (HR = 1.21, p = 0.02) and OS (HR = 1.23, p = 0.048). In vivo T-cell depletion significantly improved GFRS (HR = 0.74, p < 0.001).
Conclusion
This large study identified prognostic factors, such as age at transplant conditioning regimen, in influencing post-transplant in adult T-ALL patients undergoing allo-HSCT. Importantly, a significant improvement over time was noted. These findings hold great promise for new adapted treatment strategies and can serve as a benchmark for future studies in that setting.
Mental Health Status and Suicidality Among Bangladeshi Health Care Workers: A Year After the COVID-19 Pandemic
Authors: Alam Md. Dhedharul , Paul Sujan Kumer , Momi Mahmuda 
Published: January 2024

Abstract

Background
The coronavirus 2019 (COVID-19) cases and death tolls in Bangladesh are still rising a year after the pandemic began. However, no published data is available on mental health status and suicidality among Bangladeshi healthcare workers (HCWs) after a year of the pandemic. This study aimed to investigate the mental health status and suicidality among Bangladeshi HCWs after a year of the COVID-19 pandemic.
Methods
A cross-sectional nationwide multicentre survey was conducted in Bangladesh from March 8 to July 2, 2021. This study used the Bangla versions of the General Health Questionnaire (GHQ-12) and three COVID-19-related suicidality questions to assess mental health status and suicidality.
Results
The questionnaire was completed by a total of 2,047 HCWs from Bangladesh. The study findings indicate that the prevalence rates of mental health disorders, suicidal ideation, suicide plans, and suicide attempts were 38.6%, 3.9%, 2.4%, and 1.1%, respectively. The multivariate analysis revealed that participants who lived in urban areas with lower socioeconomic status and were single were significantly more likely to experience mental health problems and suicidal ideation. Respondents who lived with family had a significantly lower chance of experiencing mental health problems and suicidal ideation. Moreover, respondents who worked as frontline workers were significantly more likely to suffer from mental health problems, suicidal ideation, suicide plans, and suicide attempts. Moreover, it was observed that those with fewer than five years of professional experience had a considerably elevated likelihood of encountering mental health issues, while concurrently displaying a diminished probability of experiencing thoughts of suicide. In addition, respondents who exercised daily had a considerably lower risk of mental health problems and suicidal ideation.
Conclusions
The enduring impact of the COVID-19 pandemic on the mental well-being of HCWs in Bangladesh continues to be substantial, with a notable prevalence of mental health issues and suicidal tendencies. Based on identified factors, this study recommends formulating effective strategies, timely psychological support, and interventions to mitigate mental health problems and suicidality in HCWs.