bluuebunny/crossref_metadata_2025_split huggingface dataset:
Graft CD8 T‐cell‐based risk system predicts survival in antithymocyte globulin‐based myeloablative haploidentical peripheral blood stem cell transplantation
Authors: Zhu Panpan , Yang Luxin , Wu Yibo , Shi Jimin , Lai Xiaoyu , Liu Lizhen , Ye Yishan , Yu Jian , Zhao Yanmin , Yuan Xiaolin , Fu Huarui , Cai Zhen , Huang He , Luo Yi 
Published: January 2024

AbstractObjectiveThis study investigated the cellular composition of peripheral blood grafts for anti‐thymocyte globulin (ATG)‐based myeloablative haploidentical haematopoietic stem cell transplantation (haplo‐HSCT).MethodsClinical characteristics were retrospectively evaluated in a training cohort with ATG‐based myeloablative haplo‐HSCT between January 2016 and February 2020 and confirmed in a validation cohort between March 2020 and June 2021.ResultsA higher dose of graft CD8+ T cells (≥ 0.85 × 108 kg−1) was significantly improved overall survival (OS; hazard ratio [HR], 1.750; P = 0.002) and disease‐free survival (DFS; HR, 1.751; P < 0.001) in the training cohort, according to multivariate Cox regression analysis. Higher doses of mononuclear cells (MNCs) demonstrated better OS (HR, 1.517; P = 0.038) and DFS (HR, 1.532; P = 0.027). Older patient age (> 46 years), older donor age (≥ 50 years) and a higher refined disease risk index (rDRI) were also related to OS. A graft CD8+ T‐cell risk system based on graft CD8+ T‐cell dose, donor age and rDRI was constructed using a nomogram model after LASSO Cox regression analysis. It showed acceptable discrimination, with a C‐index of 0.62 and 0.63, respectively. Graft CD8+ T‐cell dose was negatively correlated with donor age (P < 0.001) and positively correlated with a higher lymphocyte percentage in the peripheral blood before mobilisation (P < 0.001).ConclusionA higher CD8+ T‐cell dose in peripheral blood‐derived grafts improves patients' survival with ATG‐based myeloablative haplo‐HSCT. Younger donors with higher lymphocyte percentages improved patients' survival with an intermediate rDRI risk.
Standardization of flow cytometric detection of antigen expression
Authors: Tian Linhua , Nelson Aaron R. , Lowe Tyler , Weaver Linda , Yuan Constance , Wang Hao‐Wei , DeRose Paul , Stetler‐Stevenson Maryalice , Wang Lili 
Published: January 2024

AbstractSince response to antigen‐based immunotherapy relies upon the level of tumor antigen expression we developed an antigen quantification assay using ABC values. Antigen quantification as a clinical assay requires methods for quality control and for interlaboratory and inter‐cytometer platform standardization. A single lot of Cytotrol™ Lyophilized Control Cells (Beckman Coulter) used for all studies. The variability in antigen quantification across 4 different instrument platforms in 2 separate laboratories was evaluated. The effect of the antibody clone utilized, importance of custom 1:1 molar ratio (fluorophore to protein, F/P) verses off‐the‐shelf antibodies, and QuantiBrite PE calibration verses linearity calibration combined with a single point scale transformation with CD4 as reference were determined. Use of single lot control cells allowed validation of reproducibility between flow cytometer platforms and laboratories and allowed assessment of different antibody lots, cocktail preparation, and different antibody clones. Off the shelf antibody preparations provide reproducible estimates of antigen density, however custom 1:1 unimolar antibody preparations should be utilized for definitive measurement of antigen expression.Geometric Mean fluorescent Intensity (GeoMFI) was not comparable across instruments and inter‐laboratory. The use of CD4 as the reference marker can minimize variability in ABC values. Comparable antigen quantification is vital in managing patients receiving antigen‐based immunotherapy. If this assay is to be utilized in a clinical setting, quality control methods have to be instituted to assure reproducibility and allow validation across laboratories. We have demonstrated that use of a lyophilized cell control is highly valuable in achieveing these goals.
Fine needle aspiration of spindle cell lipoma—Lochkern cells as a clue for diagnosis: A case arising in the parotid gland
Authors: Taweevisit Mana , Thorner Paul Scott 
Published: January 2024

AbstractSpindle cell lipoma (SCL) is a rare form of lipoma, typically occurring as a mass in the back, shoulder or posterior neck of adult males. Most cases present little diagnostic difficulty on fine needle aspiration (FNA), but can be problematic when the SCL is in an unusual location. The authors report a case in the parotid gland in a 75‐year‐old man. FNA was paucicellular and showed loose collections of spindle cells with mild to moderate atypia, admixed with ropy collagen fibers on a myxoid background. The nuclei showed occasional angulation, interpreted on FNA as suspicious for myoepithelial tumor or low‐grade sarcoma. The subsequent excisional specimen was diagnosed as SCL. On retrospective review of the FNA, an additional finding was recognized: ‘naked’ nuclei with intranuclear lipid vacuoles and positive immunostaining for S100 protein, consistent with Lochkern cells of mature adipocytes. This case highlights the challenges of diagnosing SCL on cytology when no fat‐containing cells are apparent on the smear, and stresses the significance of Lochkern cells as a clue for diagnosis.
Disproportionate cancer worries in ultra‐short‐segment Barrett's esophagus in Japan
Authors: Fukuda Sho , Watanabe Kenta , Takahashi So , Yoshida Tatsuki , Fujimori Shusei , Komatsu Taiga , Shimodaira Yosuke , Matsuhashi Tamotsu , Iijima Katsunori 
Published: January 2024

AbstractObjectivesAlthough Barrett's esophagus (BE), especially ultra‐short‐segment BE (USSBE), is very frequently diagnosed in Japan, how subjects feel about receiving a diagnosis of BE is unclear. We therefore prospectively investigated cancer worry in subjects who received a BE diagnosis.MethodsSelf‐administered questionnaires were sent to subjects who were diagnosed with BE at three health checkup institutes in Akita Prefecture, Japan. The cancer worry scale (CWS) was used to quantitatively assess the fear of developing cancer. The BE subjects were classified into USSBE <1 cm and non‐USSBE ≥1 cm groups. Factors associated with the CWS were investigated using logistic regression analyses.ResultsA total of 325 (31%) subjects, comprising 229 USSBE and 96 non‐USSBE patients were included in this study. Compared with the USSBE group, the non‐USSBE group had a significantly higher frequency of a history of a BE diagnosis and perception of carcinogenesis. However, the CWS was similar between the USSBE and non‐USSBE groups, with a median CWS of 12.5 (3.75) versus 12.7 (3.65). A multivariate logistic regression analysis revealed that while positive reflux symptoms were significantly associated with a positive CWS, the BE length was not significantly associated with it, with an odds ratio (95% confidence interval) of 1.3 (0.75–2.2).ConclusionsA BE diagnosis promotes a similar level of worry about cancer among subjects, irrespective of the length of BE. In Japan, since USSBE poses a much lower cancer risk than non‐USSBE, the former may frequently be associated with a disproportionate cancer worry relative to the latter. (UMIN000044010)
Electrochemical difference between single‐ and poly‐crystalline LaF3 for a compact and low‐price fluoride ion sensor
Authors: Kanda Kaito , Yamada Tetsuya , Yanagida Yasuko , Kubota Yuta , Matsushita Nobuhiro , Mayanagi Gen , Washio Jumpei , Takahashi Nobuhiro 
Published: January 2024

AbstractFluoride ion sensor electrodes that use polycrystalline LaF3 prepared by low‐temperature sintering can reduce sensor size and fabrication costs; however, decreasing the sintering temperature deteriorates sensor performance. Further, it is unclear why the performance of polycrystalline LaF3 prepared at low temperatures is inferior to that of the single‐crystal LaF3. Thus, in this study, we investigated factors that deteriorate sensor response under low sintering temperatures, such as solution penetration, fluorine conductivity, and ion exchange. To this end, LaF3 was prepared by changing the sintering temperature, and the electrical characteristics and sensor performance were evaluated and compared with those of the single‐crystal LaF3. The sensor performances of LaF3 sintered at 900 °C (−9.3 mV/dec) and 1000 °C (−5.8 mV/dec) were inferior to that of a single‐crystal LaF3 (−49.9 mV/dec). LaF3 prepared by sintering over 800 °C achieved fluorine removal and showed lower ion conductivity and ion exchange compared to that of the single‐crystal LaF3. LaF3 sintered at 700 °C did not show a sensor response because of solution penetration into the through‐pore. Our results confirmed that solution penetration, ion conductivity, and ion exchange influence the sensor performance of LaF3. Finally, we discussed approaches to achieve high sensor performance with LaF3 prepared by low‐temperature sintering. The proposed approaches are expected to contribute to expanding the utility of fluoride ion sensor electrodes with LaF3.
Left‐sided epileptiform activity influences language lateralization in right mesial temporal sclerosis
Authors: de Almeida Juliana Passos , Castro Bettina Martins , Passarelli Valmir , Chaim Khallil Taverna , Castro‐Lima Humberto , Listik Clarice , Jorge Carmen Lisa , Valerio Rosa , Arantes Paula Ricci , Amaro Edson , Pipek Leonardo Zumerkorn , Castro Luiz H. 
Published: January 2024

AbstractObjectiveTo investigate the association between left epileptiform activity and language laterality indices (LI) in patients with right mesial temporal sclerosis (MTS).MethodsTwenty‐two patients with right MTS and 22 healthy subjects underwent fMRI scanning while performing a language task. LI was calculated in multiple regions of interest (ROI). Data on the presence of left epileptiform abnormalities were obtained during prolonged video‐EEG monitoring.ResultsAfter correction for multiple comparisons, LI was reduced in the middle temporal gyrus in the left interictal epileptiform discharges (IED+) group, compared with the left IED− group (p < 0.05).SignificanceUsing a responsive reading naming fMRI paradigm, right MTS patients who presented left temporal interictal epileptiform abnormalities on video‐EEG showed decreased LI in the middle temporal gyrus, indicating decreased left middle temporal gyrus activation, increased right middle temporal gyrus activation or a combination of both, demonstrative of language network reorganization, specially in the MTG, in this patient population.Plain Language SummaryThis research studied 22 patients with right mesial temporal sclerosis (a specific type of epilepsy) comparing them to 22 healthy individuals. Participants were asked to perform a language task while undergoing a special brain imaging technique (fMRI). The findings showed that patients with epilepsy displayed a change in the area of the brain typically responsible for language processing. This suggests that their brains may have adapted due to their condition, altering the way language is processed.
Design and commissioning of novel test apparatus for underground structures and its application in seismic damage testing of prefabricated subway station
Authors: Chen Jinnan , Xu Chengshun , Du Xiuli , El Naggar Hesham M. , Han Runbo 
Published: January 2024

AbstractThis paper presents the design and commissioning of a novel pseudo‐static test apparatus for underground structures that accounts for soil‐structure interaction by simulating the soil with suitably designed springs. The developed apparatus was employed to conduct 1:10 large scale tests on a two‐story three‐span prefabricated subway station structure. Two comparative cyclic load tests were conducted: one involved the developed springs‐structure system; and one involved the structure alone (no springs). The test results demonstrated important differences in the damage location, damage degree, bearing capacity, and deformation capacity of the prefabricated subway station structure under the two loading conditions (i.e., with and without springs). The presence of springs (i.e., soil‐structure interaction) enhanced the lateral collapse resistance of the underground structure and affected the inter‐story displacement ratio (IDR) between the upper and lower layers of the two‐story prefabricated subway station structure. However, it did not affect the deformation coordination of the walls and columns of each layer. A finite element model of the prototype station was also established to conduct dynamic time history analysis simulating the soil‐structure interaction. The results from the dynamic analysis validated the effectiveness of the pseudo‐static test method employing the spring‐structure system. The excellent agreement between the calculated dynamic responses and the responses obtained from the pseudo static tests confirmed the ability of the developed apparatus to conduct seismic tests on complex large‐scale underground structures such as prefabricated subway stations. Thus, this test methodology might be utilized to attain valuable insights into the seismic performance of prefabricated subway stations at a relatively low cost and effort.
Gated recurrent unit network: A promising approach to corporate default prediction
Authors: Thor Michał , Postek Łukasz 
Published: January 2024

AbstractThis paper presents a promising approach using gated recurrent unit (GRU) network to predict bankruptcy based on the whole sequence of financial statements of the companies listed on an unregulated market. This approach contrasts with the traditional literature where default prediction is usually tackled with methods that do not fully account for a company's history. The GRU network can be used to model long‐term dependencies thanks to its update and reset gates, which prevent the vanishing gradient problem and decide how much of the past information is relevant for predicting a default. This aspect may be of utmost importance in alternative markets where the signal detection problem is particularly strong. The performance of the GRU network is compared against the performance of other standard machine learning methods including Cox proportional hazards, gradient‐boosted Cox proportional hazards model, extreme gradient boosting, random survival forest, and standard recurrent neural networks on the basis of a broad selection of performance metrics. The GRU network not only outperforms standard machine learning methods in out‐of‐sample forecasts but also seems to be more robust in terms of in‐ versus out‐of‐sample performance.
Fermentation of cocoa pod husks with Pleurotus salmoneo‐stramineus for food applications
Authors: Bickel Haase Thomas , Klis Victoria , Hammer Andreas Klaus , Pinto Lopez Claudia , Verheyen Christoph , Naumann‐Gola Susanne , Zorn Holger 
Published: January 2024

AbstractCocoa pod husks (CPHs), the major side‐stream from cocoa production, were valorized through fermentation with Pleurotus salmoneo‐stramineus (PSS). Considering ergosterol as a biomarker for the fungal content, the mycelium accounted for 54% of the total biomass after 8 days in submerged cultures. The crude protein content of fermented CPH (CPHF) increased from 7.3 g/100 g DM in CPH to 18.9 g/100 g DM. CPH fermentation resulted in a high biological value of 86 for the protein. The water and oil binding capacities of CPHF were 3.5 mL/g and 2.1 mL/g, respectively. The particle diameter dv,0,90 of CPHF was 373 μm as compared to 526 μm for CPH. The total dietary fiber was 73.4 g/100 g DM in CPHF and 63.6 g/100 g DM in CPH. The amount of soluble fiber was 2.3 g/100 g DM in CPHF and 10.1 g/100 g DM in CPH; the insoluble fraction accounted for 71.1 g/100 g DM and 53.6 g/100 g DM, respectively. Bread doughs with CPH or CPHF were characterized for texture, color, and farinographic properties. The dough hardness, consistency, and browning index increased with the concentration of CPH, whereas for CPHF, springiness and peak viscosities declined. We demonstrate the upcycling of CPH into nutritious and functional ingredients through PSS fermentation.
Influence of saliva on the sensory properties of US commercial smoke affected wines: Preliminary findings
Authors: Paup Victoria D. , Montero Maria L. , Ross Carolyn F. , Lee Jungmin 
Published: January 2024

AbstractPrevious research has suggested that the impact of smoke affected wines require human evaluation due to in‐mouth changes in perception, perhaps associated with saliva. Smoke affected wines (n = 36) from three major wine growing regions in the US were sourced from commercial wineries. A subset of these wines (n = 7) were evaluated by a consumer panel (n = 57) and electronic tongue (e‐tongue) to determine the influence of saliva in the sensory profile. Consumers assessed the wines for aroma and other sensory attributes, before and after individual saliva addition. Pooled saliva from consumers was used to treat all wines obtained (n = 36) and then analyzed using the e‐tongue. Results showed that saliva did not significantly alter the overall aroma, other than fruity or woody aroma liking by consumers (p > .05). However, the presence of saliva significantly lowered overall liking in both red and white wines that were affected by smoke (p ≤ .05). Consumers rated the subset of smoke affected wines below the “might purchase” category, indicating these wines were not considered acceptable by consumers. When individual pairs of smoke affected wines (before and after saliva additions) were assessed using the e‐tongue, the device was able to differentiate the pairs, validating potential usefulness to discern wine changes, though the discrimination indices were moderate to low (68.8% to 11.9%). Based on these data, in human ratings of the aroma and appearance of smoked affected wines, saliva decreased overall liking, and this was somewhat distinguishable by e‐tongue analysis.
Purification, characterization, and antioxidant ability of polysaccharides from Phascolosoma esculentas
Authors: Zhou Fengfang , Cai Binxin , Ruan Shaojiang , Wei Qi 
Published: January 2024

AbstractThe polysaccharide was extracted from Phascolosoma esculenta (PEP). Two purified polysaccharides (PEP‐1 and PEP‐2) were obtained by the column chromatography separation method. The molecular weights of PEP‐1 and PEP‐2 were 33.6 and 5.7 × 103 kDa, respectively. PEP‐1 and PEP‐2 had the same monosaccharides composition, but their molar ratios varied. The in vitro antioxidant activity of the PEP, PEP‐1, and PEP‐2 were investigated by scavenging free radicals like 3‐ethylbenzoth‐iazoline‐6‐sulfonic acid (ABTS), •OH, and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH). Additionally, the in vivo antioxidant activity of PEP‐1 was examined using the Caenorhabditis elegans (C. elegans) organism. Results showed that PEP‐1 was much more effective than PEP and PEP‐2 at scavenging DPPH, •OH, and ABTS radicals. Additionally, PEP‐1 strengthened C. elegans' ability to endure oxidative stress. PEP‐1 possessed the in vivo antioxidant capacity, including the reactive oxygen species (ROS) content reducing, and protective effect on antioxidant enzyme activities in C. elegans. In summary, PEP, PEP‐1, and PEP‐2 might have the potential to develop as functional foods and clinical medications.
Luteal phase support of intramuscular progesterone associated with lower hypertensive disorders of pregnancy as compared to vaginal progesterone: A cohort study
Authors: Li Na , Fan Lijuan , Cai He , Pan Dan , Shi Wenhao , Shi Juanzi , Wang Hui 
Published: January 2024

AbstractObjectiveTo compare the hypertensive disorders of pregnancy (HDP) risk between vaginal and intramuscular (IM) progesterone in programmed frozen–thawed embryo transfer (FET) cycles.MethodsThis was a retrospective cohort study at a tertiary hospital, and only women who achieved ongoing pregnancy after programmed FET between January 2018 and June 2022 were included. Women with chronic hypertension before pregnancy or with history of gestational hypertension or pre‐eclampsia in previous pregnancies were excluded. All women were divided into IM progesterone or vaginal progesterone groups according to the route of progesterone supplementation. Follow‐up information on obstetric complications and neonatal outcomes were obtained by telephonic interviews. The primary outcome was HDP. Association between routes of progesterone supplementation and HDP was assessed by subgroup analysis and multivariable logistic regression.ResultsA total of 5891 programmed FET cycles (3196 IM progesterone cycles and 2695 vaginal progesterone cycles) were included in the analysis. The prevalence of HDP in the IM progesterone group was significantly lower than that of the vaginal progesterone group (6.54% vs 9.17%, P < 0.001). Therein, the prevalence of gestational hypertension (3.57% vs 4.94%, P = 0.009) and pre‐eclampsia (2.97% vs 4.23%, P = 0.009) in the IM progesterone group were all significantly lower as compared to the vaginal progesterone group. According to subgroup analysis, IM progesterone was associated with lower HDP risk in all subgroups. The logistic regression analysis showed that the IM progesterone cycles were associated with lower risk of HDP compared to vaginal progesterone cycles (adjusted odds ratio 0.66, 95% CI: 0.54–0.80, P < 0.001).ConclusionAmong women undergoing programmed FET cycles, progesterone supplementation with IM progesterone was associated with reduced HDP risk compared to vaginal progesterone.
Prenatal ultrasound signs of Aarskog‐Scott syndrome in a twin pregnancy: A case report
Authors: Bertucci Emma , Giulini Simone , Sighinolfi Giovanna , Benuzzi Martina , Lugli Licia 
Published: January 2024

SynopsisWe describe the case of Aarskog‐Scott syndrome in a twin pregnancy with the prenatal finding of shortened fetal long bones.
Nanoparticle‐based therapeutic strategies for mitochondrial dysfunction in cardiovascular disease
Authors: Suzuki Isabella , Xing Huihua , Giblin Joshua , Ashraf Anisa , Chung Eun Ji 
Published: January 2024

AbstractAlthough cardiovascular diseases (CVD) are the leading cause of global mortality, there is a lack of therapies that target and revert underlying pathological processes. Mitochondrial dysfunction is involved in the pathophysiology of CVD, and thus is a potential target for therapeutic development. To target the mitochondria and improve therapeutic efficacy, nanoparticle‐based delivery systems have been proposed as promising strategies for the delivery of therapeutic agents to the mitochondria. This review will first discuss how mitochondrial dysfunction is related to the progression of several CVD and then delineate recent progress in mitochondrial targeting using nanoparticle‐based delivery systems including peptide‐based nanosystems, polymeric nanoparticles, liposomes, and lipid nanoparticles. In addition, we summarize the advantages of these nanocarriers and remaining challenges in targeting the mitochondria as a therapeutic strategy for CVD treatment.
Jahn‐Teller distortion, octahedra rotations and orbital ordering in perovskites: KScF 3 as a model system
Authors: Pascale F. , D'Arco P. , Lebègue S. , Dovesi R. 
Published: January 2024

AbstractThe KScF perovskite has been used as a model for investigating the relative importance of the Jahn‐Teller (JT) lift of degeneracy, the ScF octahedra rotation (OR), and the quadrupole‐quadrupole interaction linked to different occupancy of the Sc t subshell in various sites of the unit cell (orbital ordering, OO). The group‐subgroup sequence , , , and , supplemented by and , has been explored by using an Gaussian type basis set, hybrid functionals, and the CRYSTAL17 code. The JT lift of degeneracy provides a stabilization about 5 times larger than the sum of the OO and OR effects. The energy gained in the transition from to , consisting in a rotation of the octahedra around the axis, is 1077 E. From to , additional rotations around the and axes are possible, and the Sc electron can occupy a different t orbital, with a total energy reduction of 2318 E. The rotation of the octahedra reduces the strength of superexchange: in going from to the G‐AFM stabilization with respect to FM shrinks by a factor 4.