bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dry suction versus wet suction technique of endoscopic ultrasound-guided fine-needle biopsy for diagnosis of solid pancreatic lesions: study protocol of a multicenter randomized controlled non-inferiority trial
Authors: Xu Shenglin , Wang Junfen , Guo Jianian , Xie Fang , Qiao Weiguang , Meng Yiteng , Peng Peng , Hu Haiyan , Huang Ying , Liu Side , Qin Mengbin , Yao Jun , Li Yue 
Published: December 2023

Abstract
Background
Studies have shown that the wet suction technique in endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) generates better histological diagnostic accuracy and specimen quality than the dry suction technique. However, conclusions of wet suction on the diagnostic accuracy of endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) are still controversial. Besides, the optimal number of passes for EUS-FNB has not been determined. We aimed to design a large multicenter randomized trial to compare the diagnostic accuracy of dry suction versus wet suction technique in solid pancreatic lesions (SPLs) using 22G Franseen needles and determine the optimal number of passes required for EUS-FNB.

Methods
This is a multi-center open-label, randomized controlled non-inferiority trial with two parallel groups. Two hundred patients with SPLs will undergo EUS-FNB using 22G Franseen needles in 4 tertiary hospitals in China and will be randomly assigned to the dry suction group and wet suction group in a ratio of 1:1. The primary endpoint is diagnostic accuracy. Secondary endpoints include the optimal number of needle passes, sensitivity, specificity, specimen quality, cytological diagnoses, time of the procedure, and incidence of complications.

Discussion
This study has been designed to determine (i) whether EUS-FNB using 22G Franseen needle with dry suction is non-inferior to wet suction in terms of diagnostic accuracy and (ii) the optimal number of passes during EUS-FNB of SPLs using 22G Franseen needle.

Trial registration
ClinicalTrials.gov NCT05549856. Registered on September 22, 2022.
Glycosides from the leaves of Fraxinus Hubeiensis
Authors: Liu Xin-Yi , Tang Hong-Xia , Sheng Wen-Bing , Luo Qu-Jing , Mao Lin-Xi , Yang Yu-Pei , Guo Xiao-Zhou , Wu Qing-Lai , Jian Yu-Qing , Wang Wei , Zhou Xu-Dong 
Published: December 2023

AbstractFraxinus hubeiensis is a plant endemic to China and widely used as folk medicine to treat various diseases. However, its chemical constituents have never been reported sufficiently. Thus, the primary objective of this study was to investigate the phytochemical constituents and biological activities of F. hubeiensis leaves. Hence, combined column chromatographic and spectroscopic techniques were used to identify and characterize the secondary metabolites such as a pair of 3-keto-glycoside epimers (1) and (2), along with five known compounds (3 ~ 7). The results of α-glucosidase inhibitory activity exhibited that 1 and 2 had moderate activity with IC50 values of 359.50 and 468.43 µM, respectively, compared to a positive control acarbose with the IC50 value of 164.08 µM. However, Compounds 1–6 were shown to be inactive against the tested microbes.
Smart green spectrophotometric assay of the ternary mixture of drotaverine, caffeine and paracetamol in their pharmaceutical dosage form
Authors: Ghonim Rana , Tolba Manar M. , Ibrahim Fawzia , El-Awady Mohamed I. 
Published: December 2023

AbstractThree green and facile spectrophotometric methods were developed for the assay of Petro® components; drotaverine HCl (DRT), caffeine (CAFF), and paracetamol (PAR). The three methods depend on measuring the absorbance of the studied drugs through their ethanolic solution. The first derivative spectrophotometry (FDS) at (Δλ = 10) were good parameters for DRT and CAFF resolution; DRT and CAFF could be well calibrated using FDS at 320 and 285 nm, respectively. PAR could be estimated at 308 nm utilizing the second derivative spectrophotometry (SDS). Method II relies on the double divisor ratio derivative spectroscopy (DDRDS). The first derivative was applied on each drug where they would be assayed at 309, 288, and 255 nm for DRT, CAFF, and PAR, respectively. Method III depends on the mean centering (MCR) technique. DRT, CAFF, and PAR could be determined at 309, 214, and 248 nm, respectively. The concentrations were rectilinear in the ranges of 2–20 µg/mL for DRT, 1.5–15 µg/mL for CAFF, and 2–40 µg/mL for PAR in double devisor and mean centering but PAR from 5 to 40 µg/mL in derivative method. Method validation was performed according to ICH guidelines assured by the agreement with the comparison method. In addition, greenness assessment of the proposed methods was investigated. The application of the proposed method was extended to analyse tablet dosage form and performing invitro dissolution testing.
Lentinan has a beneficial effect on cognitive deficits induced by chronic Toxoplasma gondii infection in mice
Authors: Liu Shuxi , Yan Ziyi , Peng Yuan , Liu Yunqiu , Li Yiling , Xu Daxiang , Gong Yuying , Cui Zeyu , Wu Yongshui , Zhang Yumei , Wang Dahui , Pan Wei , Yang Xiaoying 
Published: December 2023

Abstract
Background
Toxoplasma gondii (T. gondii) is increasingly considered a risk factor for neurodegenerative diseases. However, there is only limited information on the development of drugs for T. gondii infection. Lentinan from Lentinula edodes is a bioactive ingredient with the potential to enhance anti-infective immunity. The present study aimed to investigate the neuroprotective effect of lentinan on T. gondii-associated cognitive deficits in mice.

Methods
A chronic T. gondii infection mouse model was established by administering 10 cysts of T. gondii by gavage. Lentinan was intraperitoneally administered 2 weeks before infection. Behavioral tests, RNA sequencing, immunofluorescence, transmission electron microscopy and Golgi-Cox staining were performed to assess the effect of lentinan on cognitive deficits and neuropathology in vivo. In vitro, the direct and indirect effects of lentinan on the proliferation of T. gondii tachyzoites were evaluated in the absence and presence of BV-2 cells, respectively.

Results
Lentinan prevented T. gondii-induced cognitive deficits and altered the transcriptome profile of genes related to neuroinflammation, microglial activation, synaptic function, neural development and cognitive behavior in the hippocampus of infected mice. Moreover, lentinan reduced the infection-induced accumulation of microglia and downregulated the mRNA expression of proinflammatory cytokines. In addition, the neurite and synaptic ultrastructural damage in the hippocampal CA1 region due to infection was ameliorated by lentinan administration. Lentinan decreased the cyst burden in the brains of infected mice, which was correlated with behavioral performance. In line with this finding, lentinan could significantly inhibit the proliferation of T. gondii tachyzoites in the microglial cell line BV2, although lentinan had no direct inhibitory effect on parasite growth.

Conclusions
Lentinan prevents cognitive deficits via the improvement of neurite impairment and synaptic loss induced by T. gondii infection, which may be associated with decreased cyst burden in the brain. Overall, our findings indicate that lentinan can ameliorate T. gondii-related neurodegenerative diseases.

Graphical Abstract
Laparoscopic retroperitoneal heminephrectomy for renal cell carcinoma in horseshoe kidney: a case report and review of the literature
Authors: Ngo Xuan Thai , El-Achkar Adnan , Dobbs Ryan W. , Tiong Ho Yee , Chau Quy Thuan , Tran Trong Tri , Van Dinh Le Quy , Zein Marwan , Le Nho Tinh , Truong Ho Trong Tan , Tran Tan Sang , Thai Minh Sam , Nguyen Tuan Thanh 
Published: December 2023

Abstract
Introduction
In this case report, we demonstrate our technique of a retroperitoneal laparoscopic heminephrectomy for a T1b right hilar tumor in a horseshoe kidney.

Case presentation
A 77-year-old Vietnamese woman presented to the hospital because of right flank pain. On presentation, her serum creatinine was 0.86 mg/dL and glomerular filtration rate was 65.2 mL/minute/1.73 m2. According to her renal scintigraphy, glomerular filtration rates of the right and left moieties were 24.2 and 35.5 mL/minute, respectively. Computed tomography imaging demonstrated a 5.5 × 5.0 cm solid hilar mass with a cT1bN0M0 tumor stage was in the right moiety. After discussion, the patient elected a minimally invasive surgery to treat her malignancy. The patient was placed in a flank position. We used Gaur’s balloon technique to create the retroperitoneal working space, and four trocar ports were planned for operation. Three arteries were dissected, including two arteries feeding the right moiety, one artery feeding the isthmus, and one vein, which was clipped and divided by Hem-o-lok. The isthmusectomy was performed with an Endostapler. Consequently, the ureter was clipped and divided. Finally, the whole right segment of the horseshoe kidney was mobilized and taken out via the flank incision.

Results
The total operative time was 250 min with an estimated blood loss of 200 mL. The patient's serum creatinine after surgery was 1.08 mg/dL, and glomerular filtration rate was 49.47 mL/minute/1.73 m2. The patient was discharged on postoperative day #4 without complication. Final pathologic examination of the tumor specimen revealed a Fuhrman grade II clear cell renal cell carcinoma, capsular invasion, with negative surgical margins. After a three-month follow-up, the serum creatinine was 0.95 mg/dL, and glomerular filtration rate was 57.7 mL/minute/1.73 m2. Local recurrence or metastasis was not detected by follow-up computed tomography imaging.

Conclusions
Retroperitoneal laparoscopic heminephrectomy is a safe and feasible technique for patients with renal cell carcinoma in a horseshoe kidney and may be particularly useful in low income settings without access to robotic technology.
Evaluation of energy consumption of LPWAN technologies
Authors: Rajab Husam , Al-Amaireh Husam , Bouguera Taoufik , Cinkler Tibor 
Published: December 2023

AbstractThe majority of IoT implementations demand sensor nodes to run reliably for an extended time. Furthermore, the radio settings can endure a high data rate transmission while optimizing the energy-efficiency. The LoRa/LoRaWAN is one of the primary low-power wide area network (LPWAN) technologies that has highly enticed much concentration. The energy limits is a significant issue in wireless sensor networks since battery lifetime that supplies sensor nodes have a restricted amount of energy and neither expendable nor rechargeable in most cases. A common hypothesis is that the energy consumed by sensors in sleep mode is negligible. With this hypothesis, the usual approach is to consider subsets of nodes that reach all the iterative targets. These subsets also called coverage sets, are then put in the active mode, considering the others are in the low-power or sleep mode. In this paper, we address this question by proposing an energy consumption model based on LoRa and LoRaWAN, which optimizes the energy consumption of the sensor node for different tasks for a period of time. Our energy consumption model assumes the following, the processing unit is in on-state along the working sequence which enhances the MCU unit by constructing it in low-power modes through most of the activity cycle, a constant time duration, and the radio module sends a packet of data at a specified transmission power level. The proposed analytical approach permits considering the consumed power of every sensor node element where the numerical results show that the scenario in which the sensor node transfers data to the gateway then receives an acknowledgment RX2 without receiving RX1 consumes the most energy; furthermore, it can be used to analyze different LoRaWAN modes to determine the most desirable sensor node design to reach its energy autonomy where the numerical results detail the impact of scenario, spreading factor, and bandwidth on power consumption.
Genetic association studies in critically ill patients: protocol for a systematic review
Authors: Cox Eline G. M. , Zhang Wenbo , van der Voort Peter H. J. , Lunter Gerton , Keus Frederik , Snieder Harold 
Published: December 2023

Abstract
Introduction
Patients in the intensive care unit (ICU) are highly heterogeneous in characteristics, their clinical course, and outcomes. Genetic variability may partly explain the variability and similarity in disease courses observed among critically ill patients and may identify clusters of subgroups. The aim of this study is to conduct a systematic review of all genetic association studies of critically ill patients with their outcomes.

Methods and analysis
This systematic review will be conducted and reported according to the HuGE Review Handbook V1.0. We will search PubMed, Embase, and the Cochrane Library for relevant studies. All types of genetic association studies that included acutely admitted medical and surgical adult ICU patients will be considered for this review. All studies will be selected according to predefined selection criteria, evaluated and assessed for risk of bias independently by two reviewers. Risk of bias will be assessed according to the HuGE Review Handbook V1.0 with some modifications reflecting recent insights. We will provide an overview of all included studies by reporting the characteristics of the study designs, the patients included in the studies, the genetic variables, and the outcomes evaluated.

Ethics and dissemination
We will use data from peer-reviewed published articles, and hence, there is no requirement for ethics approval. The results of this systematic review will be disseminated through publication in a peer-reviewed scientific journal.

Systematic review registration
PROSPERO CRD42021209744.
Recent progress on drugs discovery study for treatment of COVID-19: repurposing existing drugs and current natural bioactive molecules
Authors: Oktavianawati Ika , Santoso Mardi , Bakar Mohd Fadzelly Abu , Kim Yong-Ung , Fatmawati Sri 
Published: December 2023

AbstractCOVID-19 has been a major global health concern for the past three years, and currently we are still experiencing coronavirus patients in the following years. The virus, known as SARS-CoV-2, shares a similar genomic identity with previous viruses such as SARS-CoV and MERS-CoV. To combat the pandemic, modern drugs discovery techniques such as in silico experiments for docking and virtual screening have been employed to design new drugs against COVID-19. However, the release of new drugs for human use requires two safety assessment steps consisting of preclinical and clinical trials. To bypass these steps, scientists are exploring the potential of repurposing existing drugs for COVID-19 treatment. This approach involves evaluating antiviral activity of drugs previously used for treating respiratory diseases against other enveloped viruses such as HPV, HSV, and HIV. The aim of this study is to review repurposing of existing drugs, traditional medicines, and active secondary metabolites from plant-based natural products that target specific protein enzymes related to SARS-CoV-2. The review also analyzes the chemical structure and activity relationship between selected active molecules, particularly flavonol groups, as ligands and proteins or active sites of SARS-CoV-2.
Research progress of metabolomics in cervical cancer
Authors: Jia Yuhan , Zou Kun , Zou Lijuan 
Published: December 2023

Abstract
Introduction
Cervical cancer threatens women's health seriously. In recent years, the incidence of cervical cancer is on the rise, and the age of onset tends to be younger. Prevention, early diagnosis and specific treatment have become the main means to change the prognosis of cervical cancer patients. Metabolomics research can directly reflect the changes of biochemical processes and microenvironment in the body, which can provide a comprehensive understanding of the changes of metabolites in the process of disease occurrence and development, and provide new ways for the prevention and diagnosis of diseases.

Objectives
The aim of this study is to review the metabolic changes in cervical cancer and the application of metabolomics in the diagnosis and treatment.

Methods
PubMed, Web of Science, Embase and Scopus electronic databases were systematically searched for relevant studies published up to 2022.

Results
With the emergence of metabolomics, metabolic regulation and cancer research are further becoming a focus of attention. By directly reflecting the changes in the microenvironment of the body, metabolomics research can provide a comprehensive understanding of the patterns of metabolites in the occurrence and development of diseases, thus providing new ideas for disease prevention and diagnosis.

Conclusion
With the continuous, in-depth research on metabolomics research technology, it will bring more benefits in the screening, diagnosis and treatment of cervical cancer with its advantages of holistic and dynamic nature.
Role of Topical Antibiotic Ointment in the Lateral Graft following Underlay Myringoplasty: A Prospective Randomised Study
Authors: Lou Zhengcai , Lou Zihan , Lv Tian , Chen Zhengnong 
Published: December 2023

Objective The objective of this study was to compare the graft outcome and postoperative infection of with and without the use of antibiotic ointment following myringoplasty for the treatment of chronic perforations. Study Design Randomized controlled trial. Material and Methods 135 chronic perforations were prospectively randomized to use of antibiotic ointment group (UAO, n = 68) or no use of antibiotic ointment group (NAO, n = 67) following myringoplasty. The graft outcomes and postoperative infection were compared among two groups at 6 months. Results At postoperative 6 months, the graft infection rate was 4.4% in the UAO group and 10.4% in the NAO group, the difference was not significant ( P = 0.312). The graft success rates were 92.6% in the UAO group and 91.0% in the NAO group, the difference was not significant ( P = 0.979). In the UAO group, 3 patients with purulence ear discharge resulted in a residual perforation although they received ofloxacin ear drops and intravenous antibiotic therapy treatment. In the NAO group, 6 patients with purulence ear discharge resulted in a residual perforation, only one, with mild purulence discharge was successfully treated and closed. In addition, no significant between-group differences were observed pre- ( P = 0.746) or post- ( P = 0.521) operative air bone gap (ABG) values or mean ABG gain ( P = 0.745). However, granular myringitis with minimal moistness but without infection has been noted in 3 (4.4%) patients in the UAO group and in 5 (7.5%) in the NAO group, the difference was not significant ( P = 0.699). Conclusions Use and non-use of antibiotic ointments for lateral packing of graft are both comparable methods following myringoplasty for postoperative infection and graft outcomes.
Computational techniques for virtual reconstruction of fragmented archaeological textiles
Authors: Gigilashvili Davit , Lukesova Hana , Gulbrandsen Casper Fabian , Harijan Akash , Hardeberg Jon Yngve 
Published: December 2023

Abstract
Archaeological artifacts play important role in understanding the past developments of the humanity. However, the artifacts are often highly fragmented and degraded, with many details and parts missing due to centuries’ long degradation. Archaeologists and conservators attempt to reconstruct the original state of the objects either physically or virtually. This process includes characterizing and matching fragments’ features to identify which ones belong together. However, this process currently requires an extensive and tedious manual labor. Recent development in computational techniques gave rise to computer-assisted ways of virtual reconstruction, where the computer suggests solutions to the puzzle of scattered fragments and supplements or fully replaces manual labor. However, the capabilities of computational techniques remain limited in many aspects. This review summarizes the state-of-the-art computational techniques for puzzle and virtual reconstruction problems in cultural heritage applications, in general – with a particular interest in archaeological textiles. We overview existing computational methods, their applications and limitations. Afterward, based on the current knowledge gaps, we discuss where the field should go next.
Functional intervention following cardiac surgery to prevent postoperative delirium in older patients (FEEL WELL study)
Authors: Dogan Tuğce Dinç , Guttenthaler Vera , Zimmermann Alexa , Kunsorg Andrea , Dinç Merve Özlem , Knuelle Niko , Schewe Jens-Christian , Wittmann Maria 
Published: December 2023

Abstract
Background
Postoperative delirium is a common complication in patients after cardiac surgery, especially in older patients, and can manifest as a disturbance of attention and consciousness. It can lead to increased postoperative morbidity, prolonged need for care, and mortality. The presented study investigates whether the occurrence of postoperative delirium after cardiac surgery can be prevented by a multisensory stimulation. It was conducted as a prospective, randomized, controlled, non-pharmacological intervention study in the years 2021 and 2022 at the University Hospital Bonn in Germany. A total of 186 patients over 65 years with elective cardiac surgery were enrolled. Patients were randomized either to the intervention or control group. In both groups, postoperative delirium was assessed with the 3-min diagnostic interview for confusion assessment method on the first 5 days after surgery and pain was assessed using the Numeric Rating Scale. Multisensory stimulation was performed 20 min a day for the first three postoperative days in the intervention group.

Results
The incidence of postoperative delirium was 22.6% in the intervention group and 49.5% in the control group (p < 0.001). Duration of postoperative delirium was significantly shorter in the intervention group (p < 0.001). Stay in the intensive care unit was significantly longer in the control group (p = 0.006). In the regression model non-intervention, high pain scores, advanced age, and prolonged mechanical ventilation were associated with postoperative delirium (p = 0.007; p = 0.032; p = 0.006; p = 0.006, respectively).

Conclusions
Results of the study imply that a multisensory stimulation done on the first 3 days after planned cardiac surgery can reduce the incidence and duration of postoperative delirium in older patients. Influence of the treatment on the incidence of delirium in other patient groups, the length of stay in the intensive care unit, and patients´ postoperative pain should be confirmed in further clinical studies.
Trial registration: DRKS, DRKS00026909. Registered 28 October 2021, Retrospectively registered, https://drks.de/search/de/trial/DRKS00026909.