bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic analysis of a cAmpC (CMY-41)-producing Citrobacter freundii ST64 isolated from patient
Authors: Monte Daniel F M , Gonzalez-Escalona Narjol , Cao Guojie , Pedrosa Geany Targino de Souza , Saraiva Mauro M S , Balkey Maria , Jin Qing , Brown Eric , Allard Marc , Macarisin Dumitru , Magnani Marciane 
Published: February 2024

Abstract
Antibiotic resistance in Citrobacter freundii is a public health concern. This study evaluated the closed genome of a C. freundii isolated from the stool of a hospitalized patient initially related to a Salmonella outbreak. Confirmation of the isolate was determined by whole-genome sequencing. Nanopore sequencing was performed using a MinION with a Flongle flow cell. Assembly using SPAdes and Unicycler yielded a closed genome annotated by National Center for Biotechnology Information Prokaryotic Genome Annotation Pipeline. Genomic analyses employed MLST 2.0, ResFinder4.1, PlasmidFinder2.1, and VFanalyzer. Phylogenetic comparison utilized the Center for Food Safety and Applied Nutrition (CFSAN)-single nucleotide polymorphism pipeline and Genetic Algorithm for Rapid Likelihood Inference. Antimicrobial susceptibility was tested by broth microdilution following Clinical and Laboratory Standards Institute criteria. Multi-locus sequence type in silico analysis assigned the C. freundii as sequence type 64 and the blaCMY-41 gene was detected in resistome investigation. The susceptibility to antibiotics, determined using Sensititre® plates, revealed resistance to aztreonam, colistin, cefoxitin, amoxicillin/clavulanic acid, sulfisoxazole, ampicillin, and streptomycin. The genetic relatedness of the C. freundii CFSAN077772 with publicly available C. freundii genomes revealed a close relationship to a C. freundii SRR1186659, isolated in 2009 from human stool in Tanzania. In addition, C. freundii CFSAN077772 is nested in the same cluster with C. freundii clinical strains isolated in Denmark, Mexico, Myanmar, and Canada, suggesting a successful intercontinental spread.
When a Critically Ill Child is Oceans Away From a PICU: A Military Pediatric CCAT Mission
Authors: Brinkman Kevin M , Zabrocki Luke , Cadotte Noelle , Matos Renée I 
Published: February 2024

ABSTRACT
A 4-year-old former 26-week premature male presented to the U.S. Naval Hospital Guam emergency department in respiratory failure secondary to human metapneumovirus requiring urgent intubation. His condition was complicated by a bradycardic arrest requiring 15 minutes of resuscitation before the return of circulation. He was admitted to the adult intensive care unit and was managed via pediatric telecritical care from San Diego. He developed acute respiratory distress syndrome, acute renal failure, hypotension requiring multiple pressors, and fluid overload necessitating bilateral chest tubes and two peritoneal drains. A pediatric critical care air transport team departed San Antonio within 36 hours of activation and transported the patient via C-17 to Hawaii, performing a tail swap to a KC-135. Before takeoff, mechanical delays caused prolonged ground time and lack of temperature control resulted in patient’s hyperthermia to reach 104.2°F despite the ice packing. The ambient temperature caused equipment malfunction (suction, handheld blood analyzer, and ventilator), necessitating manual bagging. Despite initial temperature challenges, the team removed 700 mL of peritoneal fluid and substantially reduced the patient’s ventilator settings. After 22 hours of care, the team arrived with the patient to a civilian pediatric intensive care unit in CA, USA. Over several weeks, the patient made a full recovery. This pediatric critical care air transport mission highlights the complications intrinsic to air transport. Missions of this severity and length benefit from utilization of pediatric specialists to minimize morbidity and mortality. Highlighting the challenges related to preparation, air frame, and equipment malfunction should help others prepare for future pediatric air transports.
Impact of Overseas Deployment on Fatty Liver and Metabolic Diseases Among Korean Soldiers
Authors: Han Chang In , Lee Jaejun 
Published: February 2024

ABSTRACT

Background
The prevalence of metabolic diseases is increasing worldwide and is influenced by multiple environmental, psychological, and dietary factors. As overseas deployment (OD) of Korean soldiers brings about changes in these factors, this study aimed to explore the impact of OD on metabolic diseases.

Materials and Methods
We collected paired health checkup data of Korean soldiers between January 2020 and December 2022, before and after their deployment. We analyzed changes in lipid profile, fasting glucose, and liver function biomarkers during OD and compared the prevalence of metabolic diseases before and after the OD.

Results
A total of 2,953 soldiers were enrolled. During OD, body weight, body mass index, total cholesterol, low-density lipoprotein, and fasting glucose levels decreased significantly. Furthermore, the prevalence of obesity, impaired fasting glucose, and nonalcoholic fatty liver disease decreased significantly from 45.78% to 33.49%, P &lt; .001; 38.60% to 34.41%, P &lt; .001; and 24.89% to 18.88%, P &lt; .001, respectively. In a subgroup analysis of participants with long-term deployment (duration &gt;365 days), only the prevalence of obesity decreased significantly during deployment, whereas the prevalence of hypertension and dyslipidemia increased.

Conclusions
Metabolic biomarkers improved, and the prevalence of metabolic diseases decreased significantly during OD. However, personnel with long-term deployment did not experience significant improvements in these parameters. Further analysis is needed to identify the factors influencing these discrepancies.
From the American Jewish Conference to the Establishment of Israel: The First Jewish Zionist Grassroots Movement and President Truman
Authors: Barda Kobby 
Published: February 2024

Abstract
During the period from 1943 to 1948, the American Jewish grassroots movement organized to pressure Congress and President Truman in support of the establishment of a Jewish homeland in Palestine. This effort was led by Jewish leaders such as Rabbi Abba Hillel Silver, who worked to promote the Zionist vision of a Jewish state as proclaimed in the Balfour Declaration and endorsed by the San Remo Conference. The activities of the Jewish lobby during this period were numerous and varied. They worked to establish the American Jewish Conference, which operated in the U.S. and abroad to promote the establishment of a Jewish state in Palestine. Additionally, members used their influence in Congress and the media to push for support of the Zionist cause. Through these efforts, the Jewish lobby was able to exert significant pressure on Truman and other decision makers, ultimately leading to the creation of the State of Israel. President Truman found himself caught between the hammer and the anvil of the competing interests of Arabs and Jews, as well as conflicting views within his own administration. Despite these challenges, Truman ultimately played a crucial role in the establishment of the State of Israel in 1948.
Rapid detection of Mucorales in human blood and urine samples by functionalized Heusler magnetic nanoparticle assisted customized loop-mediated isothermal amplification
Authors: Roy Rahul , Singh Gagandeep , Dahiya Ujjwal Ranjan , Pandey Mragnayani , Xess Immaculata , Kalyanasundaram Dinesh 
Published: February 2024

Abstract
Mucormycosis is a rare disease with scarce diagnostic methods for early intervention. Available strategies employing direct microscopy using calcofluor white-KOH, culture, radiologic, and histopathologic testing often are time-intensive and demand intricate protocols. Nucleic Acid Amplification Test holds promise due to its high sensitivity combined with rapid detection. Loop-mediated isothermal amplification (LAMP) based detection offers an ultrasensitive technique that does not require complicated thermocyclers like in polymerase chain reaction, offering a straightforward means for improving diagnoses as a near-point-of-care test. The study introduces a novel magnetic nanoparticle-based LAMP assay for carryover contaminant capture to reduce false positives. Solving the main drawback of LAMP-based diagnosis techniques. The assay targets the cotH gene, which is invariably specific to Mucorales. The assay was tested with various species of Mucorales, and the limit of detections for Rhizopus microsporus, Lichtheimia corymbifera, Rhizopus arrhizus, Rhizopus homothallicus, and Cunninghamella bertholletiae were 1 fg, 1 fg, 0.1 pg, 0.1 pg, and 0.01 ng, respectively. This was followed by a clinical blindfolded study using whole blood and urine samples from 30 patients diagnosed with Mucormycosis. The assay has a high degree of repeatability and had an overall sensitivity of &gt; 83%. Early Mucormycosis detection is crucial, as current lab tests from blood and urine lack sensitivity and take days for confirmation despite rapid progression and severe complications. Our developed technique enables the confirmation of Mucormycosis infection in &lt; 45 min, focusing specifically on the RT-LAMP process. Consequently, this research offers a viable technique for quickly identifying Mucormycosis from isolated DNA of blood and urine samples instead of invasive tissue samples.
Boosting galactic outflows with enhanced resolution
Authors: Rey Martin P , Katz Harley B , Cameron Alex J , Devriendt Julien , Slyz Adrianne 
Published: February 2024

ABSTRACT
We study how better resolving the cooling length of galactic outflows affect their energetics. We perform radiative-hydrodynamical galaxy formation simulations of an isolated dwarf galaxy ($M_{\star }=10^{8}\, \mbox{M}_\mathrm{\odot }$) with the ramses-rtz code, accounting for non-equilibrium cooling and chemistry coupled to radiative transfer. Our simulations reach a spatial resolution of $18 \, \mathrm{pc}$ in the interstellar medium (ISM) using a traditional quasi-Lagrangian scheme. We further implement a new adaptive mesh refinement strategy to resolve the local gas cooling length, allowing us to gradually increase the resolution in the stellar-feedback-powered outflows, from $\ge 200 \, \mathrm{pc}$ to $18 \, \mathrm{pc}$. The propagation of outflows into the inner circumgalactic medium is significantly modified by this additional resolution, but the ISM, star formation, and feedback remain by and large the same. With increasing resolution in the diffuse gas, the hot outflowing phase ($T \gt {8} \times 10^{4} \, \mathrm{K}$) systematically reaches overall higher temperatures and stays hotter for longer as it propagates outwards. This leads to two-fold increases in the time-averaged mass and metal outflow loading factors away from the galaxy ($r=5\, \mathrm{kpc}$), a five-fold increase in the average energy loading factor, and a ≈50 per cent increase in the number of sightlines with $N_{\rm{O {\small VI}}} \ge 10^{13}\, \mathrm{cm}^{-2}$. Such a significant boost to the energetics of outflows without new feedback mechanisms or channels strongly motivates future studies quantifying the efficiency with which better-resolved multiphase outflows regulate galactic star formation in a cosmological context.
HD 12098: a highly distorted dipole mode in an obliquely pulsating roAp star
Authors: Kurtz D W , Saio H , Holdsworth D L , Joshi Santosh , Seetha S 
Published: February 2024

ABSTRACT
HD 12098 is a rapidly oscillating Ap star pulsating in the most distorted dipole mode yet observed in this class of star. Using Transiting Exoplanet Survey Satellite (TESS) Sector 58 observations, we show that there are photometric spots at both the magnetic poles of this star. It pulsates obliquely primarily in a strongly distorted dipole mode with a period of Ppuls = 7.85 min (νpuls = 183.34905 d−1; 2.12210 mHz) that gives rise to an unusual quadruplet in the amplitude spectrum. Our magnetic pulsation model cannot account for the strong distortion of the pulsation in one hemisphere, although it is successful in the other hemisphere. There are high-overtone p modes with frequencies separated by more than the large separation, a challenging problem in mode selection. The mode frequencies observed in the TESS data are in the same frequency range as those previously observed in ground-based Johnson B data, but are not for the same modes. Hence the star has either changed modes, or observations at different atmospheric depth detect different modes. There is also a low-overtone p mode and possibly g modes that are not expected theoretically with the &gt;1 kG magnetic field observed in this star.
Sub-10-nm-sized Au@AuxIr1−x metal-core/alloy-shell nanoparticles as highly durable catalysts for acidic water splitting
Authors: Wang Huimin , Chen Zhe-ning , Wang Yuanyuan , Wu Dongshuang , Cao Minna , Sun Fanfei , Cao Rong 
Published: February 2024

ABSTRACT
The absence of efficient and durable catalysts for oxygen evolution reaction (OER) is the main obstacle to hydrogen production through water splitting in an acidic electrolyte. Here, we report a controllable synthesis method of surface IrOx with changing Au/Ir compositions by constructing a range of sub-10-nm-sized core-shell nanocatalysts composed of an Au core and AuxIr1−x alloy shell. In particular, Au@Au0.43Ir0.57 exhibits 4.5 times higher intrinsic OER activity than that of the commercial Ir/C. Synchrotron X-ray-based spectroscopies, electron microscopy and density functional theory calculations revealed a balanced binding of reaction intermediates with enhanced activity. The water-splitting cell using a load of 0.02 mgIr/cm2 of Au@Au0.43Ir0.57 as both anode and cathode can reach 10 mA/cm2 at 1.52 V and maintain activity for at least 194 h, which is better than the cell using the commercial couple Ir/C‖Pt/C (1.63 V, 0.2 h).
Biofouling on titanium implants: a novel formulation of poloxamer and peroxide for in situ removal of pellicle and multi-species oral biofilm
Authors: Hussain Badra , Simm Roger , Bueno Jaime , Giannettou Savvas , Naemi Ali-Oddin , Lyngstadaas Ståle Petter , Haugen Håvard Jostein 
Published: February 2024

Abstract
Eradicating biofouling from implant surfaces is essential in treating peri-implant infections, as it directly addresses the microbial source for infection and inflammation around dental implants. This controlled laboratory study examines the effectiveness of the four commercially available debridement solutions ‘(EDTA (Prefgel®), NaOCl (Perisolv®), H2O2 (Sigma-Aldrich) and Chlorhexidine (GUM® Paroex®))’ in removing the acquired pellicle, preventing pellicle re-formation and removing of a multi-species oral biofilm growing on a titanium implant surface, and compare the results with the effect of a novel formulation of a peroxide-activated ‘Poloxamer gel (Nubone® Clean)’. Evaluation of pellicle removal and re-formation was conducted using scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy to assess the surface morphology, elemental composition and chemical surface composition. Hydrophilicity was assessed through contact angle measurements. The multi-species biofilm model included Streptococcus oralis, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans, reflecting the natural oral microbiome’s complexity. Biofilm biomass was quantified using safranin staining, biofilm viability was evaluated using confocal laser scanning microscopy, and SEM was used for morphological analyses of the biofilm. Results indicated that while no single agent completely eradicated the biofilm, the ‘Poloxamer gel’ activated with ‘H2O2’ exhibited promising results. It minimized re-contamination of the pellicle by significantly lowering the contact angle, indicating enhanced hydrophilicity. This combination also showed a notable reduction in carbon contaminants, suggesting the effective removal of organic residues from the titanium surface, in addition to effectively reducing viable bacterial counts. In conclusion, the ‘Poloxamer gel + H2O2’ combination emerged as a promising chemical decontamination strategy for peri-implant diseases. It underlines the importance of tailoring treatment methods to the unique microbial challenges in peri-implant diseases and the necessity of combining chemical decontaminating strategies with established mechanical cleaning procedures for optimal management of peri-implant diseases.
A multilevel intervention in pediatric primary care for youth tobacco control: Outcomes of implementing an Ask, Advise, and Connect model
Authors: Mays Darren , Macisco Joseph M , Hawkins Kirsten B , Sleiman Marcelo M , Yockel Mary Rose , Xie Shoulong , Phan Lilianna , Luta George , Lobo Tania , Abraham Anisha , Prokhorov Alexander V , Tercyak Kenneth P 
Published: February 2024

Abstract
Multilevel interventions in healthcare settings (e.g. Ask, Advise, and Connect; AAC) can reduce tobacco product use among adult patients: their effectiveness in pediatric practice is largely unknown. We implemented an AAC model in pediatric primary care to deter children’s tobacco use, and evaluated its effectiveness in a single-arm trial. At wellness visits, young patients (ages 12–17) completed a tablet-based assessment (Ask) of lifetime and current tobacco use. These data were made available within the electronic health record to pediatric primary care providers for preventive counseling (Advise). Providers then referred patients to an e-health evidence-based tobacco control intervention (Connect). Tobacco control outcomes were examined in the clinic population (N = 2219) and in a sample of patients (N = 388, 62% female, 39% non-White, M age = 15) over time, along with intervention engagement. Population use of tobacco products decreased following introduction of AAC (more than 2-fold). At the patient level, most children (80.9%) engaged with the intervention: those who were Black or African American, who never used tobacco products/were not susceptible to use, and who used fewer non-cigarette tobacco products were more likely to engage, but only after multiple prompts versus a single prompt. Engagement was positively associated with lowering children’s susceptibility to using tobacco at follow-up. A pediatric AAC model holds promise in deterring youth tobacco use, including among historically marginalized populations who may require additional support.
Effects of Obesity on Urogynecologic Prolapse Surgery Outcomes
Authors: Ringel Nancy E. , Lenger Stacy M. , High Rachel , Alas Alexandriah , Houlihan Sara , Chang Olivia H. , Pennycuff Jon , Singh Ruchira , White Amanda , Lipitskaia Lioudmila , Behbehani Sadikah , Sheyn David , Kudish Bela , Nihira Mikio , Sleemi Ambereen , Grimes Cara , Gupta Ankita , Balk Ethan M. , Antosh Danielle D. 
Published: February 2024

OBJECTIVE:
To systematically review the literature on outcomes of pelvic organ prolapse (POP) surgery in patients from various body mass index (BMI) categories to determine the association between obesity and surgical outcomes.

DATA SOURCES:
PubMed, EMBASE, and Cochrane databases were searched from inception to April 12, 2022; ClinicalTrials.gov was searched in September 2022 (PROSPERO 2022 CRD42022326255). Randomized and nonrandomized studies of urogynecologic POP surgery outcomes were accepted in which categories of BMI or obesity were compared.

METHODS OF STUDY SELECTION:
In total, 9,037 abstracts were screened; 759 abstracts were identified for full-text screening, and 31 articles were accepted for inclusion and data were extracted.

TABULATION, INTEGRATION, AND RESULTS
Studies were extracted for participant information, intervention, comparator, and outcomes, including subjective outcomes, objective outcomes, and complications. Outcomes were compared among obesity categories (eg, BMI 30–34.9, 35–40, higher than 40), and meta-analysis was performed among different surgical approaches. Individual studies reported varying results as to whether obesity affects surgical outcomes. By meta-analysis, obesity (BMI 30 or higher) is associated with an increased odds of objective prolapse recurrence after vaginal prolapse repair (odds ratio [OR] 1.38, 95% CI, 1.14–1.67) and after prolapse repair from any surgical approach (OR 1.31, 95% CI, 1.12–1.53) and with complications such as mesh exposure after both vaginal and laparoscopic POP repair (OR 2.10, 95% CI, 1.01–4.39).

CONCLUSION:
Obesity is associated with increased likelihood of prolapse recurrence and mesh complications after POP repair.

SYSTEMATIC REVIEW REGISTRATION:
PROSPERO CRD42022326255.
Association of Sepsis With Neurologic Outcomes of Adult Patients Treated With Venoarterial Extracorporeal Membrane Oxygnenation
Authors: Tridon Chloé , Bachelet Delphine , El Baied Majda , Eloy Philippine , Ortuno Sofia , Para Marylou , Wicky Paul-Henri , Vellieux Geoffroy , de Montmollin Etienne , Bouadma Lila , Manceau Hana , Timsit Jean-François , Peoc’h Katell , Sonneville Romain 
Published: February 2024

OBJECTIVES:
Neurologic outcomes of patients under venoarterial extracorporeal membrane oxygenation (VA-ECMO) may be worsened by secondary insults of systemic origin. We aimed to assess whether sepsis, commonly observed during ECMO support, is associated with brain injury and outcomes.

DESIGN:
Single-center cohort study of the “exposed-non-exposed” type on consecutive adult patients treated by VA-ECMO.

SETTING:
Medical ICU of a university hospital, France, 2013–2020.

PATIENTS:
Patients with sepsis at the time of VA-ECMO cannulation (“sepsis” group) were compared with patients without sepsis (“no sepsis” group). The primary outcome measure was poor functional outcome at 90 days, defined by a score greater than or equal to 4 on the modified Rankin scale (mRS), indicating severe disability or death.

INTERVENTIONS:
None.

MEASUREMENTS AND MAIN RESULTS:
A total of 196 patients were included (“sepsis,” n = 128; “no sepsis,” n = 68), of whom 87 (44.4%) had presented cardiac arrest before VA-ECMO cannulation. A poor functional outcome (mRS ≥ 4) was observed in 99 of 128 patients (77.3%) of the “sepsis” group and 46 of 68 patients (67.6%) of the “no sepsis” group (adjusted logistic regression odds ratio (OR) 1.21, 95% CI, 0.58–2.47; inverse probability of treatment weighting (IPTW) OR 1.24; 95% CI, 0.79–1.95). Subsequent analyses performed according to pre-ECMO cardiac arrest status suggested that sepsis was independently associated with poorer functional outcomes in the subgroup of patients who had experienced pre-ECMO cardiac arrest (adjusted logistic regression OR 3.44; 95% CI, 1.06–11.40; IPTW OR 3.52; 95% CI, 1.68–7.73), whereas no such association was observed in patients without pre-ECMO cardiac arrest (adjusted logistic regression OR 0.69; 95% CI, 0.27–1.69; IPTW OR 0.76; 95% CI, 0.42–1.35). Compared with the “no sepsis” group, “sepsis” patients presented a significant increase in S100 calcium-binding protein beta concentrations at day 1 (0.94 μg/L vs. 0.52 μg/L, p = 0.03), and more frequent EEG alterations (i.e., severe slowing, discontinuous background, and a lower prevalence of sleep patterns), suggesting brain injury.

CONCLUSION:
We observed a detrimental role of sepsis on neurologic outcomes in the subgroup of patients who had experienced pre-ECMO cardiac arrest, but not in other patients.