bluuebunny/crossref_metadata_2025_split huggingface dataset:
M1 Macrophage-Related Genes Model for NSCLC Immunotherapy Response Prediction
Authors: Wu Si-fan , Sheng Qi-qi , Liu Peng-jun , Jiao Zhe , Lv Jin-ru , Qiao Rong , Xie Dong-kun , Wang Zan-han , Ge Jia-mei , Li Peng-hui , Wei Tiao-xia , Lei Jie , Fan Jie-yi , Wang Liang 
Published: October 2023

2.AbstractPatients diagnosed with non-small cell lung cancer have a limited lifespan and exhibit poor immunotherapy outcomes. M1 macrophages have been found to be essential for anti-tumor immunity. This study aimed to develop an immunotherapy response evaluation model for NSCLC patients based on transcriptional expression. RNA sequencing profiles of 254 advanced-stage NSCLC patients treated with immunotherapy were downloaded from POPLAR and OAK projects. Immune cell infiltration in NSCLC patients has been examined, and thereafter different co-expressed genes were identified. Following that, the impact of M1 macrophage related genes on the prognosis of NSCLC patients was investigated. Six M1 macrophage co-expression genes, namelyNKX2-1,CD8A,SFTA3,IL2RB,IDO1, andCXCL9, exhibited a strong association with the prognosis of NSCLC and served as effective predictors for immunotherapy response. A response model was constructed using Cox regression model and Lasso Cox regression analysis. The M1 genes were validated on our previous TD- FOREKNOW NSCLC clinical trial by RT-qPCR. The response model showed excellent immunotherapy response predicting and prognosis evaluating value in advanced stage of NSCLC. The model can effectively predict advanced NSCLC prognosis and aid in identifying patients who could benefit from customized immunotherapy as well as sensitive drugs.
ATP-release pannexin channels are gated by lysophospholipids
Authors: Henze Erik , Burkhardt Russell N. , Fox Bennett W. , Schwertfeger Tyler J. , Gelsleichter Eric , Michalski Kevin , Kramer Lydia , Lenfest Margret , Boesch Jordyn M. , Lin Hening , Schroeder Frank C. , Kawate Toshimitsu 
Published: October 2023

AbstractIn addition to its role as cellular energy currency, adenosine triphosphate (ATP) serves as an extracellular messenger that mediates diverse cell-to-cell communication. Compelling evidence supports that ATP is released from cells through pannexins, a family of membrane proteins that form heptameric large-pore channels. However, the activation mechanisms that trigger ATP release by pannexins remain poorly understood. Here, we discover lysophospholipids as endogenous pannexin activators, using activity-guided fractionation of mouse tissue extracts combined with untargeted metabolomics and electrophysiology. We show that lysophospholipids directly and reversibly activate pannexins in the absence of other proteins. Secretomics experiments reveal that lysophospholipid-activated pannexin 1 leads to the release of not only ATP but also other signaling metabolites, such as 5’-methylthioadenosine, which is important for immunomodulation. We also demonstrate that lysophospholipids activate endogenous pannexin 1 in human monocytes, leading to the release of IL-1β through inflammasome activation. Our results provide a connection between lipid metabolism and purinergic signaling, both of which play major roles in immune responses.
Using long‐term data series to design adequate protected areas that ensure the conservation of inconspicuous small petrel species
Authors: De la Cruz Andrés , Pereira Jorge M. , Paiva Vitor H. , Ramos Jaime A. , Oliveira Nuno , Alonso Hany , Saavedra Camilo , Vázquez José Antonio , García‐Barón Isabel , Arcos José Manuel , Arroyo Gonzalo M. 
Published: October 2023

Abstract

Marine protected areas (MPAs) are widely used tools for conservation and management. Their correct delimitation is challenging, especially when the target species are small, elusive and inconspicuous, as little data are generally available to adequately assess their distribution at sea. Therefore, currently designated MPAs may not effectively cover key areas for small seabirds, particularly during migration and wintering seasons.

We used ensemble species distribution models (ESDMs) on a 15‐year time‐series data set of at‐sea census along the Atlantic Iberian arc to predict the potential distribution of the smallest European seabird, the European storm‐petrel (Hydrobates pelagicus), and compare it with official marine special protection areas (SPAs).

Occurrence of European storm‐petrel was related to shifts in sea surface temperature, and to small distances from the coast over the continental shelf.

Most relevant area for the species in the Atlantic Iberian arc was west‐central Portugal to north‐western coast of the Iberian Peninsula, with an additional key area in the Gulf of Cádiz. Both zones host significant SPAs, but they inadequately cover key areas for European storm‐petrels. Our findings support extending marine SPAs in the Atlantic Iberian arc to ensure their effective protection.

The distribution of the species expands over the years, varying in both size and location. These changes might be attributed to dynamic oceanographic variables, such as sea surface temperature and biomass of micronekton, which seem to play a significant role in their foraging behaviour.

Synthesis and applications. Our study highlights the importance of analysing long time series and ESDMs to design adequate protected areas, which ensure the conservation of small and highly mobile species such as storm petrels. Our results should be considered by decision‐makers to prioritise and update marine protected areas, while incorporating the dynamic nature of the ocean within an ecosystem‐based approach.
Effect of intradialytic weight resistance training exercise in sarcopenic hemodialysis patients: A randomized controlled trial
Authors: Chaovarin Chokchai , Chaiprasert Amnart , Satirapoj Bancha , Nata Naowanit , Kaewput Wisit , Tangwonglert Theerasak , Varothai Narittaya , Thimachai Paramat , Tasanavipas Pamila , Inkong Pitchamon , Siriwattanasit Narongrit , Khrongkab Natthaya , Supasyndh Ouppatham 
Published: October 2023

AbstractBackgroundSarcopenia has a high prevalence in end‐stage kidney disease (ESKD). However, there is limited evidence of resistance exercise in these patients.ObjectiveThe study investigated the effects of resistance exercise on muscle mass, strength, and physical functioning.MethodFifty‐three patients were randomly assigned to resistance training exercise (n = 26) and standard exercise (n = 27) groups. All of the patients were diagnosed with sarcopenia by the Asian Working Group for Sarcopenia 2019 criteria.ResultsAfter 12 weeks, an improvement in leg muscle strength was significantly greater in the resistant exercise group compared with standard exercise (12.19 vs. 2.83 kg, p < 0.001). Appendicular skeletal muscle mass had a mean difference (1.01 vs. 1.02 kg/m2, p = 0.96). Physical performance status had a mean difference (−2.3 vs. −18 s, p = 0.42). There were no serious adverse events.ConclusionOver a 12‐week follow‐up, resistance exercise improved muscle strength in sarcopenic ESKD patients. Muscle mass and physical performance showed no significant change, but there is still a trend demonstrating to improve.
Effects of different extraction methods on volatile profiles of flaxseed oils
Authors: Sun Xuelian , Wan Yilai , Liu Wenyu , Wei Changqing 
Published: October 2023

AbstractTo investigate the effects of different extraction methods on volatile compounds in flaxseed oil (FSO), we first carried out solvent extraction, cold pressing, and hot pressing treatments of flaxseed [Linum usitatissimum (L.)], then applied the headspace–gas chromatography–ion mobility spectrometry technology to identify the volatile substance compositions, and established flavor fingerprints of solvent‐extracted FSO, cold‐pressed FSO, and hot‐pressed FSO. In total, 81 volatile compounds were detected, including 27 aldehydes, 14 alcohols, 13 ketones, 9 heterocycles, 8 esters, 5 acids, 4 hydrocarbons, and 1 sulfur compound (dimethyl disulfide). Extraction methods had a great influence on the volatile profile of FSO. Solvent‐extracted FSO had more sweet, mild, floral, and sour volatile profiles, cold‐pressed FSO had stronger volatile profiles of winey, spicy, and fatty, and hot‐pressed FSO had green, grass, and plastic volatile profiles. Principal component analysis and Euclidean distance demonstrated that the volatile compounds of three FSO samples could be clearly distinguished. Of note, the cold‐pressed FSO and hot‐pressed FSO had similar volatile profiles, and they were different from solvent‐extracted FSO. This study could provide some guidance for improving the flavor quality of FSO and selecting the proper extraction method for FSO productions.Practical ApplicationPractical Application: This study shows extraction methods significantly affect the formation of aroma characteristics in flaxseed oil (FSO), and it provides theoretical guidance for production to use the appropriate extraction methods for high‐quality FSO.
Effects of Tremella aurantialba on physical properties, in vitro glucose release, digesta rheology, and microstructure of bread
Authors: Feng Hui , Luo Lifei , Wang Liyan , Ding Yangyue , Sun Liping , Zhuang Yongliang 
Published: October 2023

AbstractIn this study, the functional properties of a mixture consisting of Tremella aurantialba powder (TAP) and wheat flour were investigated. Further, the effects of adding 0%, 1%, 3%, 5%, and 10% TAP on the physical properties of bread, as well as its glucose release, microstructure, and rheology during in vitro simulated digestion were studied. The water‐holding, oil‐holding, and swelling capacities of wheat flour were significantly enhanced (p < 0.05) with the increase of TAP. The addition of TAP increased the hardness, chewiness, gumminess, and moisture content and darkened the color of the bread. Sensory evaluation showed that adding the 3% of TAP could produce bread that satisfies the requirements of consumers. Furthermore, adding TAP could inhibit the release of glucose from the digesta into the dialysis solution, especially the addition of 10% TAP reduced the release of bread glucose by 23.81%. This phenomenon might be related to the increased viscosity of the digesta and the smooth physical barrier on the surface of starch granules during simulated in vitro digestion of bread. Therefore, as a natural food, T. aurantialba has great potential in improving the functional properties of bread and the application of starch matrix products.
Use of non‐canonical amino acids in genetic code expansion‐based therapeutics: Effects on mouse gut microbiota
Authors: Liang Manyu , Yang Yanhong , Zhang Jing , Sun Yingjie , Peng Haixia , Yi Huawei , Wang Qin , Gao Xiaowei 
Published: October 2023

AbstractVaccines and cell therapeutics based on genetic code expansion are emerging. A crucial step in these therapeutic technologies is the oral administration of non‐canonical amino acids (ncAAs) to control pathogen growth and therapeutic protein levels in vivo. Investigating the toxicity effects of ncAAs can help identify more suitable candidates for developing genetic code expansion‐based vaccines and cell therapeutics. In this study, we determined the effects of three ncAAs, namely, 4‐acetyl‐phenylalanine (pAcF), 4‐iodo‐phenylalanine (pIoF), and 4‐methoxy‐phenylalanine (pMeoF), commonly used in genetic code expansion‐based vaccines and cell therapeutics, on the main organs, serum biochemical parameters, and gut microbiota in mice. We observed that pIoF and pMeoF significantly altered serum biochemical parameters to some extent. Moreover, the alterations in the mouse gut microbial composition were considerably greater after the oral administration of pIoF and pMeoF than after that of pAcF, compared with that in the control mice. These findings suggest that pAcF is more suitable than pIoF and pMeoF for application in genetic code expansion‐based vaccines and cell therapeutics as it disturbs the physiological and gut microecological balance in mice to a lesser extent.
Safety and efficacy of amrubicin with primary prophylactic pegfilgrastim as second‐line chemotherapy in patients with small cell lung cancer
Authors: Sekikawa Motoki , Murakami Haruyasu , Morita Meiko , Doshita Kosei , Miura Keita , Kodama Hiroaki , Morikawa Noboru , Iida Yuko , Mamesaya Nobuaki , Kobayashi Haruki , Ko Ryo , Wakuda Kazushige , Ono Akira , Kenmotsu Hirotsugu , Naito Tateaki , Chiba Hirofumi , Takahashi Toshiaki 
Published: October 2023

AbstractBackgroundAmrubicin (AMR) regimens have shown efficacy as second‐line treatment in patients with small cell lung cancer (SCLC); however, adverse events such as febrile neutropenia (FN) sometimes preclude their use. Further, the safety and efficacy of AMR with primary prophylactic pegfilgrastim (P‐PEG) have not been sufficiently evaluated. In this study, we evaluated the safety and efficacy of AMR with or without P‐PEG as second‐line chemotherapy for SCLC.MethodsWe retrospectively reviewed patients with SCLC who received AMR as second‐line chemotherapy at Shizuoka Cancer Center, between December 2014 and November 2021. Based on presence/absence of P‐PEG in their regimen, patients (n = 60) were divided into P‐PEG (n = 21) and non‐P‐PEG groups, and their clinical outcomes were evaluated.ResultsMedian of AMR treatment cycles was five (range: 1–39 cycles) in P‐PEG group and four (range: 1–15 cycles) in non‐P‐PEG group. The incidence of FN (4.8% vs. 30.8%; p = 0.02) and AMR dose reduction because of adverse events (4.8% vs. 25.6%; p = 0.08) were lower in the P‐PEG group than in the non‐P‐PEG group. The objective response rates were 52.4% and 30.8%, and median progression‐free and overall survival were 4.7 and 3.0 months, and 9.6 and 6.8 months, in the P‐PEG and non‐P‐PEG groups, respectively.ConclusionsAMR with P‐PEG as second‐line chemotherapy for SCLC reduced the incidence of FN at a maintained AMR dose intensity and was associated with favorable tumor responses and survival outcomes. P‐PEG should be considered for patients treated with AMR for SCLC including refractory relapsed SCLC.
Diet composition, adherence to calorie restriction, and cardiometabolic disease risk modification
Authors: Das Sai Krupa , Silver Rachel E. , Senior Alistair , Gilhooly Cheryl H. , Bhapkar Manjushri , Le Couteur David 
Published: October 2023

AbstractCalorie restriction (CR) is a promising approach for attenuating the risk of age‐related disease. However, the role of diet composition on adherence to CR and the effects of CR on cardiometabolic markers of healthspan remains unknown. We used the Geometric Framework for Nutrition approach to examine the association between macronutrient composition and CR adherence during the 2‐year CALERIE trial. Adult participants without obesity were randomized to a 25% CR intervention or an ad libitum intake control. Correlations of cardiometabolic risk factors with macronutrient composition and standard dietary pattern indices [Alternate Mediterranean Diet Index (aMED), Dietary Inflammatory Index (DII), and Healthy Eating Index (HEI)] were also evaluated by Spearman's correlation at each time point. The mean age was 38.1 ± 7.2 years at baseline and the mean BMI was 25.1 ± 1.7. The study population was 70% female. The CR group, but not the control, consumed a higher percentage reported energy intake from protein and carbohydrate and lower fat at 12 months compared to baseline; comparable results were observed at 24 months. Protein in the background of higher carbohydrate intake was associated with greater adherence at 24 months. There was no correlation between macronutrient composition and cardiometabolic risk factors in the CR group. However, statistically significant correlations were observed for the DII and HEI. These findings suggest that individual self‐selected macronutrients have an interactive but not independent role in CR adherence. Additional research is required to examine the impact of varying macronutrient compositions on adherence to CR and resultant modification to cardiometabolic risk factors.
Sensorineural hearing loss after cardiac surgery: a systematic review
Authors: Daniel Jonathan , Glynatsis John M. , Kovoor Joshua G. , Stretton Brandon , Bacchi Stephen , Ovenden Christopher D. , To Minh‐Son , Goh Rudy , Hewitt Joseph N. , Sahota Raguwinder S. , Chan Justin C.Y. , Ramponi Fabio , Krishnan Giri , Gupta Aashray K. 
Published: October 2023

AbstractBackgroundSensorineural hearing loss (SNHL) may occur following cardiac surgery. Although preventing post‐operative complications is vitally important in cardiac surgery, there are few guidelines regarding this issue. This review aimed to characterize SNHL after cardiac surgery.MethodThis systematic review was registered on PROSPERO and conducted in accordance with PRISMA guidelines. A systematic search of the PubMed, Embase and Cochrane Library were conducted from inception. Eligibility determination, data extraction and methodological quality analysis were conducted in duplicate.ResultsThere were 23 studies included in the review. In the adult population, there were six cohort studies, which included 36 cases of hearing loss in a total of 7135 patients (5.05 cases per 1000 operations). In seven cohort studies including paediatric patients, there were 88 cases of hearing loss in a total of 1342 operations. The majority of cases of hearing loss were mild in the adult population (56.6%). In the paediatric population 59.2% of hearing loss cases had moderate or worse hearing loss. The hearing loss most often affected the higher frequencies, over 6000 Hz. There have been studies indicating an association between hearing loss and extracorporeal circulation, but cases have also occurred without this intervention.ConclusionSNHL is a rare but potentially serious complication after cardiac surgery. This hearing loss affects both paediatric and adult populations and may have significant long‐term impacts. Further research is required, particularly with respect to the consideration of screening for SNHL in children after cardiac surgery.
The Vagrancy Concept, Border Control, and Legal Architectures of Human In/Security
Authors: Rusenko Rayna 
Published: October 2023

AbstractI answer a call by Nicholas De Genova and Ananya Roy asking scholars to investigate the historical roots of practices of illegalisation (that is, the use of law to render people illegal) and how these practices serve to re/institute a relationality between race and poverty. Using the case of regulations surrounding vagrancy in imperial Japan's metropole and its colony of Taiwan, I call attention to how illegalisation has historically been entwined with the racialised concept of the vagrant, spread globally through European anti‐vagrancy laws. I argue that this vagrancy concept was inserted into emergent border control and welfare regulations at the turn of the 20th century as a mechanism for systematically excluding racialised persons from state protection. Also, I elucidate how im/migration and social assistance schemes were historically co‐produced as legal architectures designed to provide greater socio‐economic security and status to the so‐called imperial races.
Signs of perceptual disorder during movement were reliably assessed in children with cerebral palsy in Sweden
Authors: Lidbeck Cecilia , Bartonek Åsa , Ferrari Adriano , Alboresi Silvia , Örtqvist Maria 
Published: October 2023

AbstractAimThe aim of this Swedish study was to evaluate the assessment of clinical signs of perceptual disorder in children with cerebral palsy (CP).MethodsThree experienced raters assessed 56 videos of 19 children from 1 to 18 years of age with bilateral spastic CP, which were recorded by colleagues at an Italian hospital. Six signs were evaluated for inter‐rater reliability and criterion validity. Clinical applicability was evaluated by assessing inter‐rater reliability between 47 Swedish clinicians, who examined 15 of the videos during face‐to‐face and online education seminars. There were 41 physiotherapists, two occupational therapists and four doctors, with 1–37 years of clinical experience and a median of 10 years.ResultsThe experienced raters demonstrated moderate to almost perfect inter‐rater reliability (kappa 0.54–0.81) and criterion validity (0.54–0.87) for startle reaction, upper limbs in startle position, averted eye gaze and eye blinking. The clinicians recognised these signs with at least moderate reliability (0.56–0.88). Grimacing and posture freezing were less reliable (0.22–0.35) and valid (0.09–0.50).ConclusionFour of the six signs of perceptual disorder were reliably recognised by experienced raters and by clinicians after education seminars. Extended education and larger study samples are needed to recognise all the signs.
The impact of board gender composition on loan covenant violations
Authors: Alam Md Samsul , Atif Muhammad , Cumming Douglas , Islam Md Shahidul 
Published: October 2023

AbstractResearch Question/IssueWe examine the role of board gender diversity in attenuating loan covenant violations. We also investigate whether the relationship is influenced by female independent directors. Finally, we examine the channels of this relationship.Research Findings/InsightsDrawing on gender socialization and diversity theories, our findings show that firms with gender‐diverse boards are less likely to violate loan covenants. We also find that boards with more female directors have a stronger impact on loan covenant violations than those with fewer female directors, consistent with critical mass theory. Our results also suggest that the negative relationship stems from female independent directors rather than from female executive directors. Our channel analyses indicate that the relationship is routed through covenant strictness, the financial performance of firms, and better corporate governance. Our further analysis demonstrates that the relationship is pronounced in female‐dominated industries and financially distressed firms, as well as in firms whose directors have greater experience. Our results are robust across a series of sensitivity and endogeneity tests.Theoretical/Academic ImplicationsWe contribute to an emerging strand of literature that examines the link between board gender diversity and loan covenants. We fill a gap in this stream of literature by providing the first empirical evidence that female directors in the boardroom reduce loan covenant violations through their greater integrative bargaining skills during loan deals, improving firm financial performance, and ensuring good corporate governance. Our study also contributes to the growing literature on the differential effects on corporate policies of female directors (independent and executive) and critical mass.Practitioner/Policy ImplicationsThis finding offers significant policy implications for managers, investors, and policymakers. Given the growing frequency of loan covenant violations, the presence of a gender‐diverse board should serve as a potent indicator to creditors who have a concern regarding loans. In addition, our study adds to the ongoing debate regarding the business case of board gender diversity.