bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychological risk factors predictive of suicidal distress in men receiving a community‐based brief psychological intervention
Authors: Hanlon Claire Anne , Saini Pooja , Boland Jane , McIlroy David , Poole Helen , Chopra Jennifer 
Published: February 2024

AbstractIntroductionAdaptable community‐based approaches for assessment and delivery of suicide prevention interventions for men experiencing suicidal crisis are needed. The lay your cards on the table (LYCT) component of the James' Place Model is a novel therapeutic approach comprised of four sets of card variables that correspond with suicidal risk factors. This study investigated the LYCT in predicting suicidal distress among men.MethodsCross‐sectional data of 511 men aged 18–69 years (M = 34.59 years; SD = 12.30) collected between 1st August 2018 and 29th July 2021 were assessed to predict suicidal distress measured using the CORE Clinical Outcome Measures (CORE‐OM).ResultsFrom four categories comprising the LYCT, correlational analyses demonstrated that 20 associations emerged as statistically significant (r's = 0.12–0.19). When these were included in regression analyses, effect sizes explained 2%–5% variance in CORE‐OM outcomes (R2).ConclusionUse of LYCT is supported for engaging men in the assessment of suicide risk factors and to inform tailoring of intervention delivery to suit the individual needs of men experiencing suicidal crisis.
Lymphocytic choriomeningitis arenavirus requires cellular COPI and AP-4 complexes for efficient virion production
Authors: Byford Owen , Shaw Amelia B. , Tse Hiu Nam , Todd Eleanor J. A. A. , Álvarez-Rodríguez Beatriz , Hewson Roger , Fontana Juan , Barr John N. 
Published: February 2024

ABSTRACT

Lymphocytic choriomeningitis virus (LCMV) is a bisegmented negative-sense RNA virus classified within the
Arenaviridae
family of the
Bunyavirales
order. LCMV is associated with fatal disease in immunocompromized populations, and as the prototypical arenavirus, acts as a model for the many serious human pathogens within this group. Here, we examined the dependence of LCMV multiplication on cellular trafficking components using a recombinant LCMV expressing enhanced green fluorescent protein in conjunction with a curated siRNA library. The screen revealed a requirement for subunits of both the coat protein 1 (COPI) coatomer and adapter protein 4 (AP-4) complexes. By rescuing a recombinant LCMV harboring a FLAG-tagged glycoprotein (GP-1) envelope spike (rLCMV-GP1-FLAG), we showed infection resulted in marked co-localization of individual COPI and AP-4 components with both LCMV nucleoprotein (NP) and GP-1, consistent with their involvement in viral processes. To further investigate the role of both COPI and AP-4 complexes during LCMV infection, we utilized the ARF-I inhibitor brefeldin A (BFA) that prevents complex formation. Within a single 12-h cycle of virus multiplication, BFA pre-treatment caused no significant change in LCMV-specific RNA synthesis, alongside no significant change in LCMV NP expression, as measured by BFA time-of-addition experiments. In contrast, BFA addition resulted in a significant drop in released virus titers, approaching 50-fold over the same 12-h period, rising to over 600-fold over 24 h. Taken together, these findings suggest COPI and AP-4 complexes are important host cell factors required for the formation and release of infectious LCMV.

IMPORTANCE
Arenaviruses are rodent-borne, segmented, negative-sense RNA viruses, with several members responsible for fatal human disease, with the prototypic member lymphocytic choriomeningitis virus (LCMV) being under-recognised as a pathogen capable of inflicting neurological infections with fatal outcome. A detailed understanding of how arenaviruses subvert host cell processes to complete their multiplication cycle is incomplete. Here, using a combination of gene ablation and pharmacological inhibition techniques, we showed that host cellular COPI and AP-4 complexes, with native roles in cellular vesicular transport, were required for efficient LCMV growth. We further showed these complexes acted on late stages of the multiplication cycle, post-gene expression, with a significant impact on infectious virus egress. Collectively, our findings improve the understanding of arenaviruses host-pathogen interactions and reveal critical cellular trafficking pathways required during infection.
A single-point mutation in the rubella virus E1 glycoprotein promotes rescue of recombinant vesicular stomatitis virus
Authors: Das Pratyush Kumar , Gonzalez Paulina Alatriste , Jangra Rohit K. , Yin Peiqi , Kielian Margaret 
Published: February 2024

ABSTRACT

Rubella virus (RuV) is an enveloped plus-sense RNA virus and a member of the
Rubivirus
genus. RuV infection in pregnant women can lead to miscarriage or an array of severe birth defects known as congenital rubella syndrome. Novel rubiviruses were recently discovered in various mammals, highlighting the spillover potential of other rubiviruses to humans. Many features of the rubivirus infection cycle remain unexplored. To promote the study of rubivirus biology, here, we generated replication-competent recombinant VSV-RuV (rVSV-RuV) encoding the RuV transmembrane glycoproteins E2 and E1. Sequencing of rVSV-RuV showed that the RuV glycoproteins acquired a single-point mutation W448R in the E1 transmembrane domain. The E1 W448R mutation did not detectably alter the intracellular expression, processing, glycosylation, colocalization, or dimerization of the E2 and E1 glycoproteins. Nonetheless, the mutation enhanced the incorporation of RuV E2/E1 into VSV particles, which bud from the plasma membrane rather than the RuV budding site in the Golgi. Neutralization by E1 antibodies, calcium dependence, and cell tropism were comparable between WT-RuV and either rVSV-RuV or RuV containing the E1 W448R mutation. However, the E1 W448R mutation strongly shifted the threshold for the acid pH-triggered virus fusion reaction, from pH 6.2 for the WT RuV to pH 5.5 for the mutant. These results suggest that the increased resistance of the mutant RuV E1 to acidic pH promotes the ability of viral envelope proteins to generate infectious rVSV and provide insights into the regulation of RuV fusion during virus entry and exit.

IMPORTANCE
Rubella virus (RuV) infection in pregnant women can cause miscarriage or severe fetal birth defects. While a highly effective vaccine has been developed, RuV cases are still a significant problem in areas with inadequate vaccine coverage. In addition, related viruses have recently been discovered in mammals, such as bats and mice, leading to concerns about potential virus spillover to humans. To facilitate studies of RuV biology, here, we generated and characterized a replication-competent vesicular stomatitis virus encoding the RuV glycoproteins (rVSV-RuV). Sequence analysis of rVSV-RuV identified a single-point mutation in the transmembrane region of the E1 glycoprotein. While the overall properties of rVSV-RuV are similar to those of WT-RuV, the mutation caused a marked shift in the pH dependence of virus membrane fusion. Together, our studies of rVSV-RuV and the identified W448R mutation expand our understanding of rubivirus biology and provide new tools for its study.
Telomere-to-telomere genome assembly of the aflatoxin biocontrol agent
Aspergillus flavus
isolate La3279 isolated from maize in Nigeria
Authors: Legan Andrew W. , Mehl Hillary L. , Wissotski Marina , Adhikari Bishwo N. , Callicott Kenneth A. 
Published: February 2024

ABSTRACT

Here, we report the complete genome of the non-aflatoxigenic
Aspergillus flavus
isolate La3279, which is an active ingredient of the aflatoxin biocontrol product Aflasafe. The chromosome-scale assembly clarifies the deletion pattern in the aflatoxin biosynthesis gene cluster and corrects a misidentified assembly previously published for this isolate.
Complete genome sequences of mycobacteriophages Ageofdapage, Aubs, BABullseye, CheetoDust, ShaboiShabazz, and TomBrady
Authors: Bass Shelbie , Coates Danielle , Frasketi Nina , James Smeetha , Marella Vishnu , Pulla Riya , Stoecker Kyle , Tupurani Srichandra , Johnson Allison A. 
Published: February 2024

ABSTRACT

We report genome sequences of six mycobacteriophages. Each virus was isolated from a soil sample and belongs to the siphovirus morphology. Genomes are 41,901–60,613 bp in length, contain between 62 and 103 protein-coding genes, with up to 40% of those genes having a predicted function.
Microbial community interactions determine the mineralization of soil organic phosphorus in subtropical forest ecosystems
Authors: Pan Chang , Sun Chenchen , Qu Xinjing , Yu Wenruinan , Guo Jiahuan , Yu Yuanchun , Li Xiaogang 
Published: February 2024

ABSTRACT

In subtropical forest ecosystems with few phosphorus (P) inputs, P availability and forest productivity depend on soil organic P (Po) mineralization. However, the mechanisms by which the microbial community determines the status and fate of soil Po mineralization remain unclear. In the present study, soils were collected from three typical forest types: secondary natural forest (SNF), mixed planting, and monoculture forest of Chinese fir. The P fractions, Po-mineralization ability, and microbial community in the soils of different forest types were characterized. In addition, we defined Po-mineralizing taxa with the potential to interact with the soil microbial community to regulate Po mineralization. We found that a higher labile P content persisted in SNF and was positively associated with the Po-mineralization capacity of the soil microbial community.
In vitro
cultures of soil suspensions revealed that soil Po mineralization of three forest types was distinguished by differences in the composition of fungal communities. We further identified broad phylogenetic lineages of Po-mineralizing fungi with a high intensity of positive interactions with the soil microbial community, implying that the facilitation of Po-mineralizing taxa is crucial for soil P availability. Our dilution experiments to weaken microbial interactions revealed that in SNF soil, which had the highest interaction intensity of Po-mineralizing taxa with the community, Po-mineralization capacity was irreversibly lost after dilution, highlighting the importance of microbial diversity protection in forest soils. In summary, this study demonstrates that the interactions of Po-mineralizing microorganisms with the soil microbial community are critical for P availability in subtropical forests.

IMPORTANCE
In subtropical forest ecosystems with few phosphorus inputs, phosphorus availability and forest productivity depend on soil organic phosphorus mineralization. However, the mechanisms by which the microbial community interactions determine the mineralization of soil organic phosphorus remain unclear. In the present study, soils were collected from three typical forest types: secondary natural forest, mixed planting, and monoculture forest of Chinese fir. We found that a higher soil labile phosphorus content was positively associated with the organic phosphorus mineralization capacity of the soil microbial community. Soil organic phosphorus mineralization of three forest types was distinguished by the differences in the composition of fungal communities. The positive interactions between organic phosphorus-mineralizing fungi and the rest of the soil microbial community facilitated organic phosphorus mineralization. This study highlights the importance of microbial diversity protection in forest soils and reveals the microbial mechanism of phosphorus availability maintenance in subtropical forest ecosystems.
The histone deacetylase Hda1 affects oxidative and osmotic stress response as well as mycoparasitic activity and secondary metabolite biosynthesis inTrichoderma atroviride
Authors: Speckbacher Verena , Flatschacher Daniel , Martini-Lösch Nora , Ulbrich Laura , Baldin Clara , Bauer Ingo , Ruzsanyi Veronika , Zeilinger Susanne 
Published: February 2024

ABSTRACTThe mycoparasitic fungusTrichoderma atrovirideis applied in agriculture as a biostimulant and biologic control agent against fungal pathogens that infest crop plants. Secondary metabolites are among the main agents determining the strength and progress of the mycoparasitic attack. However, expression of most secondary metabolism-associated genes requires specific cues, as they are silent under routine laboratory conditions due to their maintenance in an inactive heterochromatin state. Therefore, histone modifications are crucial for the regulation of secondary metabolism. Here, we functionally investigated the role of the class II histone deacetylase encoding genehda1ofT. atrovirideby targeted gene deletion, phenotypic characterization, and multi-omics approaches. Deletion ofhda1did not result in obvious phenotypic alterations but led to an enhanced inhibitory activity of secreted metabolites and reduced mycoparasitic abilities ofT. atrovirideagainst the plant-pathogenic fungiBotrytis cinereaandRhizoctonia solani. The∆hda1mutants emitted altered amounts of four volatile organic compounds along their development, produced different metabolite profiles upon growth in liquid culture, and showed a higher susceptibility to oxidative and osmotic stress. Moreover,hda1deletion affected the expression of several notable gene categories such as polyketide synthases, transcription factors, and genes involved in the HOG MAPK pathway.IMPORTANCEHistone deacetylases play crucial roles in regulating chromatin structure and gene transcription. To date, classical—Zn2+dependent—fungal histone deacetylases are divided into two classes, of which each comprises orthologues of the two sub-groups Rpd3 and Hos2 and Hda1 and Hos3 of yeast, respectively. However, the role of these chromatin remodelers in mycoparasitic fungi is poorly understood. In this study, we provide evidence that Hda1, the class II histone deacetylases of the mycoparasitic fungusTrichoderma atroviride, regulates its mycoparasitic activity, secondary metabolite biosynthesis, and osmotic and oxidative stress tolerance. The function of Hda1 in regulating bioactive metabolite production and mycoparasitism reveals the importance of chromatin-dependent regulation in the ability ofT. atrovirideto successfully control fungal plant pathogens.
Supplemental Material: Early Cretaceous continental arc magmatism in the Wakhan Corridor, South Pamir: Mantle evolution and geodynamic processes during flat subduction of the Neo-Tethyan oceanic slab
Authors: Yang Fan , al. et 
Published: February 2024

<p>Text S1: Analytical methods. Table S1: LA-ICP-MS zircon U-Pb isotopic dating data for the Kalaqigu diorite and monzogranite at the Chinese Wakhan Corridor, South Pamir. Table S2: Representative electron probe analyses of amphibole, biotite, and plagioclase from the Kalaqigu pluton. Table S3: Whole-rock major (wt%) and trace (ppm) elements and Sr-Nd-Pb isotopic compositions of Kalaqigu diorite and monzogranite at the Chinese Wakhan Corridor and Taxkorgan S-type monzogranites. Table S4: Zircon Lu-Hf isotopic compositions of Kalaqigu diorite at the Chinese Wakhan Corridor, South Pamir. Table S5: Zircon O isotopic compositions of Kalaqigu diorite and monzogranite at the Chinese Wakhan Corridor, South Pamir. </p>
Use of drugs for hyperlipidaemia and diabetes and risk of primary and secondary brain tumours: nested case–control studies using the UK Clinical Practice Research Datalink (CPRD)
Authors: Robinson Jamie W , Martin Richard , Ozawa Mio , Elwenspoek Martha Maria Christine , Redaniel Maria Theresa , Kurian Kathreena M , Ben-Shlomo Yoav 
Published: February 2024

ObjectivesPrevious studies have suggested that fibrates and glitazones may have a role in brain tumour prevention. We examined if there is support for these observations using primary care records from the UK Clinical Practice Research Datalink (CPRD).DesignWe conducted two nested case–control studies using primary and secondary brain tumours identified within CPRD between 2000 and 2016. We selected cases and controls among the population of individuals who had been treated with any anti-diabetic or anti-hyperlipidaemic medication to reduce confounding by indication.SettingAdults older than 18 years registered with a general practitioner in the UK contributing data to CPRD.ResultsWe identified 7496 individuals with any brain tumour (4471 primary; 3025 secondary) in total. After restricting cases and controls to those prescribed any anti-diabetic or anti-hyperlipidaemic medication, there were 1950 cases and 7791 controls in the fibrate and 480 cases with 1920 controls in the glitazone analyses. Longer use of glitazones compared with all other anti-diabetic medications was associated with a reduced risk of primary (adjusted OR (aOR) 0.89 per year, 95% CI 0.80 to 0.98), secondary (aOR 0.87 per year, 95% CI 0.77 to 0.99) or combined brain tumours (aOR 0.88 per year, 95% CI 0.81 to 0.95). There was little evidence that fibrate exposure was associated with risk of either primary or secondary brain tumours.ConclusionsLonger exposure to glitazones was associated with reduced primary and secondary brain tumour risk. Further basic science and population-based research should explore this finding in greater detail, in terms of replication and mechanistic studies.
Role of modifiable organisational factors in decreasing barriers to mental healthcare: a longitudinal study of mission meaningfulness, team relatedness and leadership trust among Canadian military personnel deployed on Operation LASER
Authors: Born Jennifer , Liu Aihua , Sudom Kerry , Michaud Kathy , Xi Min , Fikretoglu Deniz 
Published: February 2024

ObjectivesThe literature presents complex inter-relationships among individual-factors and organisational-factors and barriers to seeking mental health support after deployment. This study aims to quantify longitudinal associations between such factors and barriers to mental health support.DesignA longitudinal online survey of Canadian Armed Forces (CAF) personnel collected data at 3 months post-deployment (T1), 6 months post-deployment (T2) and 1 year post-deployment (T3).SettingIn 2020, as part of Canada’s response to the COVID-19 pandemic, 2595 CAF personnel deployed on Operation LASER to support civilian long-term care facilities in Québec and Ontario.ParticipantsAll Operation LASER personnel were invited to participate: 1088, 582 and 497 responded at T1, T2 and T3, respectively. Most respondents were young, male, non-commissioned members.Main outcome measuresBarriers to mental health support were measured using 25 self-reported items and grouped into theory-based factors, including eight factors exploring care-seeking capabilities, opportunities and motivations; and two factors exploring moral issues. Logistic regressions estimated the crude and adjusted associations of individual and organisational characteristics (T1) with barriers (T2 and T3).ResultsWhen adjusting for sex, military rank and mental health status, increased meaningfulness of deployment was associated with lower probability of endorsing barriers related to conflicts with career goals and moral discomfort in accessing support at T2. Higher scores in trust in leadership were associated with lower probability of endorsing four barriers at T2, and five barriers at T3.ConclusionsWe identified several modifiable organisational-level characteristics that may help reduce perceived barriers to mental health support in military and other high-risk occupational populations. Results suggest that promoting individuals’ sense of purpose, instilling trust in leadership and promoting relatedness among team members may improve perceptions of access to mental health supports in the months following a domestic deployment or comparable occupational exposure.
Effectiveness of musculoskeletal manipulations in patients with neck pain: a protocol for a systematic review and network meta-analysis
Authors: Gong Zhichao , Gao Yinyan , Liu Wenqi , Liu Xiaowei , Chen Qintao , Zhang Siyi , Liu Lei , Li Jiangshan , Li Wu 
Published: February 2024

IntroductionNeck pain is a common problem that severely affects physical and mental health. While musculoskeletal manipulations are recommended as the first-line treatment for adults with neck pain, the comparative effectiveness of different musculoskeletal manipulations remains unclear. This systematic review and network meta-analysis of randomised controlled trials (RCTs) will compare the effectiveness of different types of musculoskeletal manipulations, with the overarching aim of guiding clinical practice.Methods and analysisTwo independent reviewers will search four English electronic databases (Web of Science, Cochrane Library, EMBASE, PubMed) and three Chinese electronic databases (China National Knowledge Infrastructure, China Science and Technology Journal Database, Wanfang) for relevant RCTs published from 1 January 2013 to 30 April 2023. The Clinical Trials Registry (ClinicalTrials.gov) will be searched for completed but unpublished RCTs. English and Chinese will be used to search English databases and Chinese databases, respectively. RCTs of musculoskeletal manipulations for adults (aged ≥18 years) with neck pain will be considered eligible for inclusion. A pairwise meta-analysis and network meta-analysis will be performed, and pooled risk ratios, standardised mean differences and 95% CIs will be determined.Ethics and disseminationEthics approval is not required as this study is a literature review. The results of this review will be published in peer-reviewed journals or disseminated at conferences.PROSPERO registration numberCRD42023420775.
Home palliative medicine: an audit of weekend and public holiday telephones on-call
Authors: Mercadante Sebastiano , Bellavia Giuseppe , Intravaia Giuseppe 
Published: February 2024

ObjectivesTo report the on-call activity of a well-established home care programme.MethodsThe charts of consecutive phone calls recorded in the weekend or public holidays were reviewed.ResultsThe principal reason to call were based on caregivers’ uncertainty, for the need of information or just to be reassured. The other more frequent reason to call was related to technical problems with peripheral intravenous central catheters.ConclusionsAn integrated system of ‘on call’ allows to select the interventions, covering most of the patients’ needs.
Convergent close-coupling calculations of positron scattering from neon and argon
Authors: Mori N. A. , Scarlett L. H. , Bray I. , Fursa D. V. 
Published: February 2024

Abstract
Positron scattering from the noble gas atoms neon and argon is calculated using the single-center convergent close-coupling (CCC) method. Unlike previous CCC calculations, we have relaxed the frozen-core approximation by fully opening the outermost s and p shells, and find better agreement with experiment for excitation energies and dipole polarizability. We have used this improved structure model to calculate elastic, elastic differential, total, momentum-transfer, electron-loss, and total bound excitation cross sections. We have applied a complex model potential calculation, scaled to our single-center CCC results, to obtain direct ionization and positronium-formation cross sections for these systems. This complex model potential was also used to calculate results between the positronium-formation and ionization thresholds, giving complete results from $$10^{-5}$$

10

-
5

eV to 5000 eV. Overall, good agreement is observed with past theory and experiment for most transitions.

Graphic abstract