bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of cropping patterns in the Bhimsagar canal irrigation scheme using linear programming approach
Authors: Rajput Jitendra , Choudhary Ramnarayan , Kothari Mahesh 
Published: January 2025

ABSTRACT
Optimization techniques can be employed to determine the best cropping patterns to ensure optimum net benefits, food production, and labor employment. Therefore, the current investigation employed the linear programming module in LINGO 14.0 software to develop optimal crop area allocation plans in the Right Main Canal (RMC) of the Bhimsagar irrigation project. The optimal cropping patterns (OCPs) were generated for 2013–2014 under different canal run (CR) periods in a month, i.e., 30, 24, and 21 days using three objective functions, viz., net benefit, food production, and labor-days maximization. In addition, the OCP scenarios were generated for long-term agriculture sustainability scenarios, viz., 2015, 2020, and 2025. Results revealed that the net benefit was increased by 122.2, 93.4, and 95.5% for Ratanpura (RT), Chaplada (CP), and Maraita II (MT) minor, respectively, under OCP estimated for 30-day CR in 2013–2014, whereas food production was increased by 22.4, 33.8, and 25.9% for RT, CP, and MT, respectively, under OCP over existing cropping patterns. Similarly, under OCP, labor employment had increased by 40.9, 33.8, and 33.1% for RT, CP, and MT, respectively, for 30-day CR. These findings infer that higher net benefits, food production, and labor employment may be achieved by shifting to OCPs.
Research Progress of Microneedles in Vaccine Delivery
Authors: Qiao Xinyu , Liu Derun , Pan Wentao , He Meilin , Meng Fanda 
Published: January 2025

Abstract:
Large-scale infectious diseases have become a significant threat to human
health and safety. The successful invention of vaccines is the most powerful means for
preventing infectious diseases and has greatly improved global human health. Even during
the pandemic of COVID-19, which has affected the world, vaccines have played an
irreplaceable role. Microneedles (MNs) punctured the stratum corneum and formed microchannels
in the skin allowing the vaccine to be efficiently recognized by the abundant
antigen-presenting cells (APCs) in the skin to form specific immunity. Compared
with traditional vaccination methods, MN transdermal immunization has the advantages
of painlessness, easy storage, and efficient immune response. In this review, we summarize
the types of vaccines, types of MNs, research progress and clinical research status
of MN-based vaccines. We also cover various technologies for vaccine encapsulation,
stable delivery of MN vaccines, and a wide range of potential clinical applications.
We also outline the future development prospects of the MN system onboard to achieve
better clinical benefits.
Identification of Ferroptosis-related Genes for Diabetic Nephropathy by
Bioinformatics and Experimental Validation
Authors: Song Siyuan , Yu Jiangyi 
Published: January 2025

Objective:
The present study delves into the exploration of diagnostic biomarkers linked with ferroptosis in the context of diabetic nephropathy, unraveling their underlying molecular mechanisms.

Methods:
In this study, we retrieved datasets GSE96804 and GSE30529 as the training cohort, followed by
screening for Differentially Expressed Genes (DEGs). By intersecting these DEGs with known ferroptosisrelated genes, we obtained the differentially expressed genes related to ferroptosis (DEFGs). Subsequently,
Weighted Correlation Network Analysis (WGCNA) was carried out to identify key modules associated with
Diabetic Nephropathy (DN), culminating in the identification of a significant gene. Enrichment analysis and
Gene Set Enrichment Analysis (GSEA) were then carried out on the DEFGs and genes linked to the significant gene. To validate our findings, we employed cohorts GSE30528 and GSE43950, utilizing ROC curve
analysis to assess diagnostic efficacy for DN, as measured by the area under the curve (AUC). Immune cell
infiltration was analyzed and compared between groups using the CIBERSORT algorithm. Bayesian colocalization analysis was performed to examine the co-location of DEFGs and DN. Finally, to validate the hub
genes identified, we conducted quantitative real-time polymerase chain reaction (qRT-PCR) experiments in
vitro.

Results:
FUZ, GLI1, GLI2, GLI3, and DVL2 were identified as the hub genes. Functional enrichment analysis demonstrated that ferroptosis and immune response play an important role in DN. ROC analysis showed
that the identified genes had good diagnostic efficiency in DN. The results of the immune infiltration analysis
showed that there may be crosstalk between ferroptosis and immune cells in DN. Bayesian co-localization
analysis revealed the genetic correlation between the hub genes and DN. The outcomes of the qRT-PCR analyses corroborated the reliability of the identified hub genes as robust molecular markers for targeted therapy
in DN.

Conclusion:
The interplay between immune inflammatory reactions and ferroptosis emerges as a crucial
pathogenic mechanism, offering novel insights into the molecular therapy of DN. Furthermore, the identification of FUZ, GLI1, GLI2, GLI3, and DVL2 as potential targets holds promise for future therapeutic interventions aimed at treating DN.
mRNA Vaccines: Unlocking Potential, Exploring Applications, and Envisioning Future Horizons
Authors: Mishra Gaurav , Rathee Sunny , Garg Munish , Patil Umesh K. 
Published: January 2025

Abstract:
In recent years, there have been notable strides in developing mRNA vaccines, resulting in
the creation of potent immunizations against diverse diseases. This review examines the most recent
advancements in this field, focusing on their implications for future vaccine development. The pursuit
of heightened vaccine efficacy is investigated through cutting-edge methods in adjuvant selection,
delivery system optimization, and antigen selection. The review also explores the potential for
personalized vaccines based on genetic profiles, along with the latest techniques to ensure vaccine
stability and extend shelf life. Highlighting the versatility of mRNA vaccines in addressing emerging
infectious diseases and their variations, the review underscores the significance of swift response
plans and advanced technologies to counter evolving viral mutations. In summary, this in-depth analysis
emphasizes how mRNA vaccines hold transformative potential in reshaping both therapeutic and
preventive strategies. Notable achievements include the creation of extremely potent mRNA vaccinations
against the SARS-CoV-2 virus, resulting in the COVID-19 pandemic. Ongoing efforts to address
challenges like long-term immune protection and increase the effectiveness and stability of
mRNA vaccines are also discussed. This review's main goal is to provide a thorough summary of
current advancements in mRNA vaccine technology while exploring how these advances may impact
future approaches to treating and preventing different diseases.
CDK8 as a therapeutic target for overall survival prediction in cervical squamous cell carcinoma (CESC)
Authors: Xie Feiting , Zhu Qiugang , Yang Shizhou , Jin Xiaofen , Ruan Danqi , Wang Lingfang , Li Yang , Ren Fang , Chen Xiaojing 
Published: January 2025

Abstract:
Cyclin-Dependent Kinase 8 (CDK8) is a paracrine transcriptional regulator involved in regulating cellular stress response, growth, and neurological functions in conjunction with mediator complex subunits 12 (MED12), MED13, and cyclin C. Studying the relationship be-tween CDK8 and cervical squamous cell carcinoma (CESC) has significant clinical implications in diagnosis, treatment, and prognosis. Therefore, we analyzed the relationship between CDK8 and poor prognosis of CESC. The results indicated that CDK8 was overexpressed in CESC, and the survival rate of patients in the CDK8 hypermethylation group was higher than that in the CDK8 hypomethylation group. In addition, CDK8 was associated with immune cell infiltration in tumor tissues. Overall, these findings provide more evidence for the relationship between CDK8 and a patient’s overall survival, which could provide insights into clinical diagnosis and prognosis prediction for CESC.
The Use of Portable Device for Measuring Nasal FeNO in Subjects
with Allergic Rhinitis - A Single Center Study
Authors: Duong-Quy Sy , Le-Khac Bao , Craig Timothy , Nguyen-Hong Duc , Tang-Thi-Thao Tram , Nguyen-Tuan Anh , Nguyen-Van Toi , Nguyen-Thi-Hong Lien , Pham-Van Linh , Pham-Huy Quyen , Pham-Van Thuc , Vu-Tran-Thien Quan , Nguyen-Nhu Vinh , Vo-Pham-Minh Thu 
Published: January 2025

Objective:
This study aimed to demonstrate the accuracy of a portable device for measuring nasal and bronchial fractional exhaled nitric oxide (nFeNO and FeNO) as an alternative
method to replace a high-cost multiple flow device in subjects with allergic rhinitis (AR) .

Methods:
It was a descriptive and comparative study. The healthy subjects (control group), subjects with AR (AR group) and those with AR and asthma (AR-A group) were enrolled in this
study. They underwent nasal and bronchial FeNO measurements by both fixed flow NObreath ®
connected to nasal mask (for nFeNO) and without mask (for FeNO) and multiple flows HypAir
FeNO ®
.

Results:
There were 153 subjects recruited during the study period, including 63 healthy subjects
and 90 subjects with AR and AR-A. There were no statistically significant differences in anthropometric characteristics between study groups. The concentrations of nasal FeNO and bronchial
FeNO measured by a portable device in subjects with AR were higher than in healthy subjects:
76.0 ± 50.7 ppb vs 36.0 ± 28.1 ppb ( P < 0.01), and 32.3 ± 15.4 ppb vs 9.2 ± 5.6 ppb ( P < 0.001).
There was a weak correlation between nasal FeNO measured by portable devices and multiple
flow devices.

Conclusions:
The portable device is useful in measuring nasal FeNO in subjects with AR. The advantages of a low-cost and accurate portable device may help it be
used in primary care in the management of subjects with allergic rhinitis and asthma
Determining the Association between Preeclampsia and the Thyroid
Hormone Status in Pregnant Women
Authors: Salari Saeideh , Zamani Saeed , Raesi Rasoul , Hushmandi Kiavash , Daneshi Salman 
Published: January 2025

Background:
Preeclampsia is a significant multisystem disorder in pregnancy care that
hormonal, environmental, and genetic factors can cause.

Aim:
This study aimed to investigate the relationship between preeclampsia and thyroid hormone
levels in pregnant women.

Method:
This case-control study was conducted in 2022 with 200 pregnant women in southeast
Iran, utilizing a convenience sampling method. The participants included 100 women in the
preeclampsia group and 100 in the normal pregnancy group. Data related to preeclampsia were obtained from the Integrated Health System (SIB) at the health center in Jiroft city, while information
about the thyroid hormones was extracted from the Ministry of Health's SIB system. The data were
analyzed using SPSS-26 software, employing both descriptive and inferential statistical tests

Results:
Systolic pressure was significantly higher in the preeclampsia group (149.14 mm Hg) than
in the control group (105.33 mm Hg), and diastolic pressure also showed a significant difference
(94.57 mm Hg in preeclampsia vs. 64.33 mm Hg in normal pregnancies). Thyroid-stimulating hormone (TSH) levels were significantly higher in the preeclampsia group (3.7) compared to the normal pregnancy group (2.5), indicating a
notable difference (P<0.005). Thyroid thyroxine (TT4) levels were significantly lower in the preeclampsia group (10.78) compared to the control group (12.5)
(P<0.005).

Conclusion:
Thyroid function significantly impacts the risk and severity of preeclampsia, with elevated TSH and reduced TT4 levels indicating potential
complications. Monitoring these hormone
levels is crucial for managing affected pregnancies. It is recommended that health policymakers include thyroid function screening in prenatal care protocols.
Diagnostic Accuracy Comparison between Automatic and Conventional Blood Pressure Measuring Devices in Adults with Hypertension
Authors: Curi-Curi Pedro José , Santiago-González Nicolás , Valencia-Ledezma Omar Esteban , García-Rascon Rafael , Hernandez-Roque Elia , Oliver-Flores Evelin Y. , Curiel-Ramirez Guadalupe 
Published: January 2025

Background
Blood pressure measurement is a main clinical procedure used for the detection of Systemic Arterial Hypertension. Mercury sphygmomanometer, in combination with auscultation technique, is still recognized as the gold standard for non-invasive blood pressure measurement.

Objective
The objective of this study is to compare the diagnostic accuracy between automatic non-invasive blood pressure measurement devices versus mercury sphygmomanometers in hospitalized patients.

Methods
A prospective, analytical and cross-sectional diagnostic method study was designed to compare the concordance, positive and negative predictive value, the sensitivity and specificity of automatic non-invasive blood pressure measurement devices and the mercury sphygmomanometer. The devices were divided into two groups: the reference standard which was mercury sphygmomanometer (n=150), versus the index tests, that were wrist device (n=150), semi-automatic sphygmomanometer (n=150) and vital signs monitor (n=150).

Results
The monitor detected more cases (69%) of systemic arterial hypertension (p 0.0019, OR 5.3). The semiautomatic sphygmomanometer identified more true positives (n=22) and true negatives (n=113). It also showed a higher diagnostic accuracy: 67% sensitivity (p 0.0576, OR 0.5) and 97% specificity (p 0.088, OR 3.2). A mean arterial pressure variation <5mmHg of 65% was found with the semiautomatic sphygmomanometer (p <0.0001, OR 0.02) and 61% with the vital signs monitor (p <0.0001, OR 0.12). Best concordance of blood pressure between reference standard versus index test was obtained in the following order: Semi-automatic sphygmomanometer, Vital signs monitor, and Wrist device.

Conclusion
The use of vital signs monitor to measure blood pressure is recommended in all patients, although semiautomatic sphygmomanometer is more sensitive for the detection of systemic arterial hypertension.
Optimizing Distribution Transformer Ratings in the Presence of Electric Vehicle Charging and Renewable Energy Integration
Authors: Argade Sachin , Vashikar Vishal , Anantwar Harsha , Chavan Sudarshan L. 
Published: January 2025

Aims and Background:
A re-evaluation of distribution transformer ratings is necessary
to ensure efficient and reliable operation due to the significant impact of the growing number of
electric vehicles (EVs) on load dynamics. The objective of this study is to optimize the rating of
the distribution transformers to accommodate the year-round demand for electric vehicle charging
while minimizing expenses and no-load losses. The performance and lifespan of transformers under
dynamic hourly loads are evaluated by analysing real-world EV charging data, load profiles,
and transformer parameters. Using state-of-the-art simulation, real-world data analysis, and optimization
algorithms, this study optimizes the transformer distribution ratings under the integration
of EV charging and renewable energy.

Objectives and Methodology:
Dynamic hourly load changes can be captured by analysing realworld
electric vehicle charging data in conjunction with seasonal load profiles. In order to maximise
transformer lifetime and minimise losses, convex optimisation is subjected to Karush-Kuhn-
Tucker (KKT) conditions. Transformer performance is also assessed by using energy storage devices
and solar energy simulations. To determine the impact of various electric vehicle charging
scenarios on thermal stress and transformer ageing, sensitivity analyses are performed.

Results and Discussion:
Transformers with a 10% higher rating can manage maximum electric
vehicle charging loads with a 15% slower loss of life acceleration, according to our models.
Transformer performance is further optimised with the integration of solar energy and energy
storage systems, which improves load control and reduces operational costs by up to 20%.

Conclusion:
Using a convex optimisation framework and the Karush-Kuhn-Tucker (KKT) criteria,
the study achieves a 95% accuracy rate in predicting hourly load fluctuations.
The difficult laparoscopic cholecystectomy: a bailout strategy
Authors: Chooklin S.M. , Chuklin S.S. 
Published: January 2025

Laparoscopic cholecystectomy is one of the most frequent surgeries today. Given the procedure’s extensive adoption by surgeons across all skill levels, from novices to experts, it becomes imperative to develop guidelines to ensure its safety for patients. Since complexi-ty of surgeries differ in each person, ranging from simple to intricate cases involving a frozen Calot’s triangle, it is extremely important to perform laparoscopic surgery carefully to avert any potential harm. Given its global prevalence, even a 0.1 % complication rate translates to a significant figure. Therefore, practicing safe cholecystectomy is advocated strongly. This review presents modern approaches to laparoscopic cholecystectomy in difficult cases — bailout surgery — to prevent damage to the bile ducts and vessels. In particular, the features of subtotal cholecystectomy, fundus-first cholecystectomy, and infundibular dissection are considered.
Effect of ranolazine on cardiac remodeling and pulmonary artery pressure in patients with acute pulmonary embolism
Authors: Tseluyko V.Y. , Kurinna M.V. , Kinoshenko Ye.I. 
Published: January 2025

Background. The development of right ventricular (RV) dysfunction is the most significant predictor of in-hospital mortality and one of the most important factors affecting the short- and long-term prognosis of pulmonary embolism (PE). Therefore, the development of treatment regimens aimed at inhibiting the progression of RV dysfunction and the fastest possible restoration of its function, as well as preventing the development and progression of pulmonary hypertension, is an important area of reducing mortality in acute PE. One of the most promising drugs that can theoretically reduce both RV and pulmonary damage is ranolazine. The purpose of the study was to evaluate the feasibility and efficacy of prescribing ranolazine to patients with PE, focusing on its impact on echocardiographic parameters. Materials and methods. The study included 70 patients diagnosed with acute PE verified bу CT pulmonary artery angiogram performed on the first 1–2 days and after 8–10 days of treatment. Patients were divided into two groups: group 1 consisted of 29 people who received ranolazine at a dose of 500 mg twice daily starting from day 1–2 of standard treatment, for 1 month; group 2 included 49 patients who did not receive ranolazine. All patients underwent a standard general clinical examination with an assessment of risk factors for PE and early death according to the 2019 European Society of Cardiology guidelines, with subsequent statistical processing of the data. Results. When analyzing the dynamics of indicators in the groups, a statistically significant decrease was found in the size of the right atrium (p = 0.0009), RV (p = 0.046), mean pulmonary artery pressure (mPAP) (p = 0.027), pulmonary artery systolic pressure (sPAP) (p = 0.00004) in the group 1. In the group without ranolazine, there also was a decrease in the size of the right atrium (p = 0.002), mPAP (p = 0.009), and sPAP (p = 0.03), but with no effect on the size of the RV. When comparing the difference in parameters on day 1 and 8–10, i.e., the shift of the parameters during treatment, a statistically significant decrease in sPAP was obtained in the ranolazine group compared to the group without its use (p = 0.03). Conclusions. Adding ranolazine to standard therapy for pulmonary embolism reduces right ventricular and atrial remodeling, as well as pulmonary artery pressure.
Optimization of acute postoperative pain management in trauma and orthopedic patients
Authors: Shostak M.A. , Domoratskyi O.E. 
Published: January 2025

Background. The modern approach to multimodal postoperative analgesia involves the administration of two or more analgesic agents that exert a polymodal effect on the pathogenetic mechanisms of pain. Nefopam hydrochloride can prevent opioid-induced hyperalgesia, relieve pain without respiratory depression and without affecting platelet aggregation, the state of the gastrointestinal mucosa and intestinal motility, which differentiates it from traditional non-opioid analgesics. Objective: to assess the impact of nefopam hydrochloride as a component of multimodal analgesia on pain intensity and stress response markers in trauma and orthopedic patients during postoperative pain management. Materials and methods. A prospective randomized open-label controlled clinical study was conducted in 120 patients (47 men, 73 women) aged 19 to 94 years (mean (M ± σ) of 55.97 ± 19.78 years) who underwent lower limb surgeries under spinal anesthesia. Patients were divided into two groups: the controls (n = 60) received standard multimodal analgesia (ketorolac, acetaminophen), while people in the main group (n = 60) additionally received 20 mg of nefopam hydrochloride intramuscularly 4 times per day. Pain intensity was evaluated using the Numeric Rating Scale, narcotic analgesic consumption, and cortisol levels in blood serum were assessed as well. Data analysis was performed using SPSS 20 (SPSS Inc.), version 21.0.0 for Windows. Results. It was found that the use of nefopam hydrochloride as a component of multimodal postoperative analgesia in patients undergoing trauma and orthopedic surgeries significantly reduces score on the Numeric Rating Scale: by 23.6 % during the first 6 hours after surgery (p < 0.001), and by 1.5, 2.3, and 3.6 times at 12, 18, and 24 hours postoperatively, respectively (p < 0.001). It was also associated with lower mean serum cortisol concentrations — 5.20 ± 4.43 vs. 8.51 ± 3.48 µg/dL in the control group 6 hours after surgery, and 2.08 ± 1.63 vs. 4.11 ± 1.70 µg/dL in 12 hours (p < 0.001). Additio-nally, it reduced the average cumulative morphine dose by 1.5 times during the first 24 hours postoperatively (p < 0.001). The opioid-reducing effect of multimodal analgesia with nefopam hydrochloride reached 61.7 %. Conclusions. Nefopam hydrochloride is an effective component of multimodal postoperative analgesia in trauma and orthopedic patients, improving the quality of postoperative pain management and reducing stress response intensity.