bluuebunny/crossref_metadata_2025_split huggingface dataset:
Presence of neutralizing SARS‐CoV‐2 antibodies in asymptomatic population of N'Djamena, Chad
Authors: Wondeu Andrillene Laure Deutou , Abakar Mahamat Fayiz , Frasca Federica , Nodjikouambaye Aleyo Zita , Abdelrazakh Fatima , Naibei Nathan , Dzomo Guy Rodrigue Takoudjou , Djimtoibaye Djallaye , Mad‐Toingue Joseph , Scagnolari Carolina , Antonelli Guido , Linardos Giulia , Russo Cristina , Perno Carlo Federico , Yandai Fissou Henry , Atturo Sabrina , Hiscott John , Colizzi Vittorio , Cappelli Giulia , Ngueadoum Nambatibe , Haroun Alsadick , Choua Ouchemi , Moussa Ali Mahamat 
Published: January 2024

AbstractIntroductionNeutralizing antibodies (NAbs) are an important specific defence against viral infections, as these antibodies bind to specific receptor(s) and block the viral entry. NAbs assessments are therefore useful in determining individual or herd immunity to SARS‐CoV‐2. This study aims to deepen the investigation by assessing the positivity rate of neutralizing anti‐spike antibodies to understand the real protection of the studied population against SARS‐CoV‐2.MethodsThis study involved 260 plasma samples from a larger cohort of 2,700 asymptomatic volunteer donors, enrolled between August and October 2021 in health facilities of N'Djamena. In this study four different kits and techniques including the pseudotype assay have been used and compared with detect the SARS‐CoV‐2 antibodies. Pseudotyped vesicular stomatitis virus (VSV), was used both the identify and measure the NAbs that to evaluate the performance of two cheaper and easy to use commercial kits, specific for the detection of receptor‐binding domain antibodies (anti‐RBD) against the SARS‐CoV‐2 spike protein.ResultsThe VSV spike neutralization assay showed that 59.0% (n = 59) samples were positive for NAbs with titers ranging from 1:10 to 1:4800. While 23 out the 41 negative NAbs samples were detected positive using anti‐RBD (Abbott) test. Furthermore, a direct and significant strong correlation was found between NAbs and anti‐RBD, specifically with Abbott kit. Taken together, the Roche and Abbott methods indicated agreement at the high concentrations of antibodies with the VSV‐pseudovirus method. Abbott and Roche indicated a good sensitivity, but the Abbott system test appeared to have better specificity than the Roche test.ConclusionOur findings indicated a high presence of NAbs against SARS‐CoV‐2 spike protein among asymptomatic individuals in N'Djamena. This could be one of the reasons for the low severity of Covid‐19 observed in this area, given the key role of NAbs in blocking SARS‐CoV‐2 infection.
A case of severe ureteral injury repaired by renal autotransplantation with an iliac vein patch using bovine pericardium
Authors: Hisano Mizuki , Matsuura Tomohiko , Koizumi Junichi , Ito Akito , Kato Renpei , Maekawa Shigekatsu , Kanehira Mitsugu , Sugimura Jun , Kin Hajime , Obara Wataru 
Published: January 2024

IntroductionRenal autotransplantation is considered a surgical procedure for extensive ureteral defects. Herein, we report a case of severe ureteral injury repaired by laparoscopic nephrectomy and renal autotransplantation with an iliac vein patch using bovine pericardium.Case presentationA 56‐year‐old woman who had previously undergone gynecological surgery complained of right‐sided abdominal pain. She was then later diagnosed with a right middle ureteral injury with a 5‐cm long defect. We performed retroperitoneal laparoscopic nephrectomy and renal autotransplantation. As the iliac vein was fragile, venous patching using bovine pericardium was performed. The patient's renal function was well preserved after surgery.ConclusionLaparoscopic nephrectomy and renal autotransplantation is an effective method for repairing severe ureteral injury with the preservation of renal function. A venous patch using bovine pericardium might be considered as a replacement for a fragile vein.
Integrating nutrition and genetic counseling: A case study approach to interprofessional learning
Authors: Cratsenberg Drew M. , Jackson Mariah K. , Hanson Corrine K. , Zimmerman Holly H. 
Published: January 2024

AbstractInterprofessional collaboration is an increasingly important skillset for practicing healthcare professionals including genetic counselors and registered dietitian nutritionists. A multi‐part interactive case study activity was created to develop interprofessional skills for graduate students within genetic counseling and medical nutrition training programs at an academic medical center. Feedback from learners who participated in this activity highlights its effect on their post‐graduation clinical practice. Additionally, since the implementation of this activity, collaboration between students and faculty members of each program has occurred in other scholarly pursuits, fostering longstanding interprofessional relationships. Similar approaches to interprofessional education could be considered with other healthcare professions or at other institutions as another tool to strengthen practice upon graduation.
Trends in comorbid chronic kidney disease and atrial fibrillation‐related cardiovascular mortality in the United States
Authors: Ibrahim Ramzi , Singh Vikram J. , Tan Min Choon , Singh Simar J. , Shahid Mahek , Elchouemi Mohanad , Gianni Carola , Lee Justin Z. , Al‐Ahmad Amin 
Published: January 2024

AbstractBackgroundThe impact of chronic kidney disease (CKD) on atrial fibrillation outcomes (AF) is not well understood.MethodsWe conducted analyses of comorbid AF and CKD related death in the United States from 1999 to 2020 using descriptive epidemiology.ResultsAge‐adjusted mortality rates (AAMR) per 100,000 increased from 0.39 in 1999 to 1.65 in 2020. Non‐Hispanic populations (1.01) and nonmetropolitan areas (1.08) had higher AAMRs compared to Hispanic (0.62) and metropolitan (0.97) areas. Midwestern (1.11) and Western (1.13) US regions recorded the highest AAMRs.ConclusionsThese findings highlight the need for interventions to address AF death disparities in patients with CKD.
Release or transection of superficial medial collateral ligament during open‐wedge high tibial osteotomy demonstrated similar clinical outcomes and valgus laxity
Authors: Jung Se‐Han , Choi Chong‐Hyuk , Kim Sungjun , Jung Min , Chung Kwangho , Jeong Hyunjun , Kim Sung‐Hwan 
Published: January 2024

AbstractPurposeTo analyse whether valgus laxity and clinical outcomes differ depending on whether the superficial medial collateral ligament (sMCL) is released or transected during medial open‐wedge high tibial osteotomy (MOWHTO).MethodsConsecutive patients who underwent MOWHTO and subsequent radiological follow‐up for at least 2 years were retrospectively evaluated. The patients were divided into release and transection groups, according to the sMCL manipulation technique. Each patient was assessed for the following variables on valgus stress radiographs taken before surgery and at the 12‐ and 24‐month follow‐ups: the absolute value of valgus (ABV) and side‐to‐side difference (SSD) between the affected and normal sides. The differences between preoperative SSD and those at 12 and 24 months were respectively calculated and defined as delta SSD (ΔSSD). The Visual Analogue Scale, Lysholm knee, International Knee Documentation Committee subjective, and Knee Injury and Osteoarthritis Outcome scores were used to evaluate patient‐reported outcomes.ResultsEighty‐five patients were included in the study. Forty‐two patients (49.6%) underwent sMCL release, and the remaining 43 patients (50.4%) underwent sMCL transection. No significant differences were observed in the ABV and SSD of valgus laxity at the different time points between the two groups (n.s.). Furthermore, no significant differences were observed in the ΔSSD at the 12‐ and 24‐month follow‐ups between the two groups (n.s.). Significant improvement from preoperative values was observed in all patient‐reported outcomes (p < 0.001), with no significant differences between the two groups at any time point (n.s.).ConclusionSignificant improvements in clinical outcomes were observed, regardless of the technique used. Postoperative valgus laxity did not occur with either technique. The transection technique, which can be performed more simply and quickly, demonstrated similar clinical outcomes and valgus laxity to the release technique.Level of EvidenceLevel III.
Processing and quality evaluation of dry beans (Phaseolus vulgaris) in flexible pouches
Authors: Wang Weijia , Siddiq Muhammad , Dolan Kirk D. , Cichy Karen A. 
Published: January 2024

AbstractDry beans are a nutrient‐dense food with a history of processing in metal cans to improve convenience for consumers. Flexible retort pouches have been gaining popularity as a food package to replace metal cans because they are lighter in weight and require less energy to process. While there are potential benefits of using pouches for processed bean products, a pilot‐scale pouch processing protocol for beans is needed for testing to expand to wider commercial applications. To address that need, the objectives of this study were to optimize a pouch processing method for dry beans and assess the subsequent quality of pouch‐processed beans. Black and kidney bean genotypes from different field trials and production years and harvested by different threshing methods were used in this study. Pouch processing was conducted with two types of pouches at a pilot facility, and processing quality included texture, appearance, and color. Cooking time of dry seeds was assessed to understand how variety and environmentally induced differences in cooking time influence pouch processing quality. Kidney beans that required longer dry seed cooking times had firmer texture when pouch processed (r = .72, p < .01). Both genotype and production year impacted cooking time and pouch processing quality. Threshing method had a significant impact on the appearance of processed kidney beans, while it had no significant impact on the processing quality of black beans. Pouch processing requires 42% (at 245°F/118.3°C retort temperature) or 60% (at 250°F/121°C) less retort processing time compared with canning. Both foil and non‐foil pouches can be used to differentiate varieties for processing quality, but the non‐foil pouches require much less effort to seal. This study provides a detailed methodology for pouch processing and quality evaluation of dry beans and useful information for researchers and processors in future applications of using pouches as alternative packaging for processed beans.
The Ellrott grab: A small, lightweight sediment sampler for collecting undisturbed sandy sediments
Authors: Moncada Chyrene , Ellrott Andreas , de Beer Dirk , Amann Rudolf , Knittel Katrin 
Published: January 2024

AbstractSampling sandy surface sediments is an important first step in understanding biogeochemical processes in these dynamic environments. However, sampling such sediments poses several challenges, especially when undisturbed samples with porewater are required. Several grab samplers are commercially available, but they are either prone to sample loss, too heavy or bulky for use in small vessels, or those with spring‐loaded mechanisms present safety issues. Here, we present the Ellrott grab, a lightweight sediment sampler designed for collecting undisturbed surface sediments including porewater and overlying bottom seawater. The sampler consists of a frame and a rotating bowl that can collect 370 cm2 of surface sediments up to 10 cm deep (2.5 liters total volume). The instrument is 40 × 60 cm in size, has a basic weight of 10 kg, with up to 20 kg additional weights for stability in sandy sediments. Two persons can operate the grab and it can be used on small boats with a crane and winch system or a hand winch. The grab is now in routine use in the Wadden Sea and in Isfjorden, Svalbard. The samples obtained from the grab were suitable for various geochemical and microbial analyses. Using microelectrodes, we found that in situ oxygen profiles were similar to ex situ profiles in cores subsampled from the grab, confirming that the grab causes minimal disturbance to the sample. Although the grab was designed for collecting sandy sediments, it could also be applied to silty sediments, allowing straightforward and efficient sampling of various sediment types.
Microgel‐Crosslinked Thermo‐Responsive Hydrogel Actuators with High Mechanical Properties and Rapid Response
Authors: Yang Yanyu , Xiao Ying , Wu Xiang , Deng Junjie , Wei Rufang , Liu Ashuang , Chai Haiyang , Wang Rong 
Published: January 2024

AbstractSmart hydrogels responsive to external stimuli are promising for various applications such as soft robotics and smart devices. High mechanical strength and fast response rate are particularly important for the construction of hydrogel actuators. Herein, tough hydrogels with rapid response rates are synthesized using vinyl‐functionalized poly(N‐isopropylacrylamide) (PNIPAM) microgels as macro‐crosslinkers and N‐isopropylacrylamide as monomers. The compression strength of the obtained PNIPAM hydrogels is up to 7.13 MPa. The response rate of the microgel‐crosslinked hydrogels is significantly enhanced compared with conventional chemically crosslinked PNIPAM hydrogels. The mechanical strength and response rate of hydrogels can be adjusted by varying the proportion of monomers and crosslinkers. The lower critical solution temperature (LCST) of the PNIPAM hydrogels could be tuned by copolymerizing with ionic monomer sodium methacrylate. Thermo‐responsive bilayer hydrogels are fabricated using PINPAM hydrogels with different LCSTs via a layer‐by‐layer method. The thermo‐responsive fast swelling and shrinking properties of the two layers endow the bilayer hydrogel with anisotropic structures and asymmetric response characteristics, allowing the hydrogel to respond rapidly. The bilayer hydrogels are fabricated into clamps to grab small objects and flowers that mimicked the closure of petals, and it shows great application prospects in the field of actuators.
circRNA TATA‐box binding protein associated factor 15 acts as an oncogene to facilitate bladder cancer progression through targeting miR‐502‐5p/high mobility group box 3
Authors: Yang Hong , He Peilin , Luo Wei , Liu Shaoyou , Yang Yong 
Published: January 2024

AbstractCircular RNAs (circRNAs) are key in regulating bladder cancer progression. This study explored the effects of circRNA TATA‐box binding protein associated factor 15 (circTAF15) on bladder cancer progression. We enrolled 80 bladder cancer patients to examine the relationship between circTAF15 expression and clinical features. The function of circTAF15 on bladder cancer cell viability, proliferation, migration, invasion, and glycolysis was monitored by cell counting kit‐8 assay, 5‐Ethynyl‐2′‐deoxyuridine experiment, Transwell experiment, and glycolysis analysis. Dual luciferase reporter gene assay, RNA pull‐down assay, and RNA immunoprecipitation assay were used to verify the binding between circTAF15 and miR‐502‐5p or between miR‐502‐5p and high mobility group box 3 (HMGB3). circTAF15 effect on in vivo growth of bladder cancer was investigated by xenograft tumor experiment. Quantitative real‐time polymerase chain reaction, Western blot, and immunohistochemistry were implemented to investigate the expression levels of genes. circTAF15 was upregulated in bladder cancer patients, associated with unfavorable outcomes. circTAF15 knockdown attenuated bladder cancer cell viability, proliferation, migration, invasion, epithelial‐mesenchymal transition, and glycolysis. circTAF15 suppressed miR‐502‐5p expression, and miR‐502‐5p inhibited HMGB3 expression. Low miR‐502‐5p expression was associated with unfavorable outcomes in bladder cancer patients. miR‐502‐5p silencing and HMGB3 overexpression counteracted the inhibition of circTAF15 knockdown on the malignant phenotype of bladder cancer cells. circTAF15 knockdown attenuated the in vivo growth of bladder cancer cells. circTAF15 enhanced the progression of bladder cancer through upregulating HMGB3 via suppressing miR‐502‐5p. circTAF15 may be a novel target to treat bladder cancer in the future.
Retrospectively gated ultrashort‐echo‐time MRI T1 mapping reveals compromised pulmonary microvascular NO‐dependent function in a murine model of acute lung injury
Authors: Kwiatkowski Grzegorz , Czyzynska‐Cichon Izabela , Tielemans Birger , Geerkens Lotte , Jasztal Agnieszka , Velde Greetje Vande , Chłopicki Stefan 
Published: January 2024

This study sought to develop noninvasive, in vivo imaging schemes that allow for quantitative assessment of pulmonary microvascular functional status based on the combination of pulmonary T1 mapping and dynamic contrast‐enhanced (DynCE) imaging.Ultrashort‐echo‐time (UTE) imaging at 9.4 T of lung parenchyma was performed. Retrospective gating was based on modulation of the first point in each recorded spoke. T1 maps were obtained using a series of five consecutive images with varying RF angles and analyzed with the variable flip angle approach. The obtained mean T1 lung value of 1078 ± 38 ms correlated well with previous reports. Improved intersession variability was observed, as evident from a decreased standard deviation of motion‐resolved T1 mapping (F‐test = 0.051). Animals received lipopolysaccharide (LPS) and were imaged at t = 2, 6, and 12 h after administration. The nitric oxide (NO)‐dependent function was assessed according to changes in lung T1 after L‐NAME injection, while microvascular perfusion and oxidant stress were assessed with contrast‐enhanced imaging after injection of gadolinium or 3‐carbamoyl‐proxyl nitroxide radical, respectively.Retrospectivel gated UTE allowed robust, motion‐compensated imaging that could be used for T1 mapping of lung parenchyma. Changes in lung T1 after L‐NAME injection indicated that LPS induced overproduction of NO at t = 2 and 6 h after LPS, but NO‐dependent microvascular function was impaired at t = 12 h after LPS. DynCE imaging at t = 6 h after LPS injection revealed decreased microvascular perfusion, with increased vascular permeability and oxidant stress.MRI allows to visualize and quantify lung microvascular NO‐dependent function and its concomitant impairment during acute respiratory distress syndrome development with high sensitivity. UTE T1 mapping appears to be sensitive and useful in probing pulmonary microvascular functional status.
Late‐Stage Oral Cancer Detection After California and Illinois Restored Medicaid Dental Benefits
Authors: Semprini Jason T. 
Published: January 2024

AbstractObjectivePrevious research found an association between California's Medicaid dental coverage and oral cancer detection. However, this relationship has yet to be explored in other states or by subgroup populations.Study DesignIn addition to controlling for sociodemographic and tumor characteristics, this study implemented a traditional difference‐in‐differences design to compare distant‐stage diagnosis trends in states restoring Medicaid dental benefits (California [CA] and Illinois [IL]) with trends in states with constant Medicaid dental benefits.SettingThis retrospective, observational study analyzed oral cavity and pharynx cancer case data from The Surveillance, Epidemiology, and End Results program (2004‐2017).MethodsThe outcome was a binary variable indicating whether a patient was diagnosed at a distant stage. Subgroup analyses were conducted by state, race/ethnic group, sex, age, and county‐level household income.ResultsThe sample included 109,997 adults diagnosed with cancer of the oral cavity and pharynx. Restoring Medicaid dental benefits was associated with a statistically significant 2.7%‐point decline in the probability of a distant‐stage oral cancer diagnosis. This estimate represented a 14% relative change from baseline rates. Results were consistent for CA and IL and by county‐level median income. Estimates were significantly larger for adults under age 65, males, and adults identifying as Hispanic; non‐Hispanic Black; American Indian; or Asian American or Pacific Islander.ConclusionRestoring Medicaid dental coverage improved early detection in both CA and IL, with the greatest reductions in distant‐stage diagnoses occurring in younger adults, males, and minoritized racial/ethnic groups. Future research should investigate whether earlier detection reduces oral cancer mortality disparities.
Effect of addition of fillers (MoS2 and PFA) on the tribological and mechanical properties of biobased semi‐aromatic polyamide (PA10T)
Authors: Zhu Rongli , Pu Zejun , Zheng Lin , Zheng Pan , Wu Fang , Yu Dayang , Wang Xu , Yue Mengjie , Yang Yuhao , Yang Kaijie , Wu Linkai , Pang Jiahong , Meng Chihan , Peng Qiuxia , Zhong Jiachun 
Published: January 2024

AbstractIn an attempt to enhance the properties of biobased semi‐aromatic polyamide poly (decamethylene terephthalamide) acid (PA10T), the effects of different proportions of fillers [MoS2 and polyfluoroalkoxy (PFA)] were investigated. A range of stable and wear‐resistant composites, with filler contents varying from 0 to 10 wt%, were successfully prepared using a one‐step extrusion blending method. To analyze the chemical structures, Fourier transform infrared spectroscopy (FT‐IR) experiments were conducted. Additionally, SEM, tribological tests, differential scanning calorimetry (DSC), x‐ray diffraction (XRD), thermogravimetric analyzer (TGA), tensile measurement, notched impact test, and bending test were employed to thoroughly evaluate the properties of both PA10T and composites. The findings demonstrated that the target products were effectively created, and the composites with fillers exhibited superior wear resistance. Alongside this, they showcased comparable heat resistance and mechanical properties to the pure PA10T. Of particular note, when 4 wt% PFA and 6 wt% MoS2 were added, the specific wear rate of PA10T‐F4M6 (0.0016 mg·min−1) was impressively reduced by 86.2% in comparison to pure PA10T (0.0116 mg·min−1). This reduction in wear mass from 0.7 mg (PA10T) to 0.1 mg (PA10T‐F4M6) further confirms the improvement in wear resistance. Ultimately, these results suggest that composites, with their exceptional wear resistance, hold great potential as heat‐resistant engineering thermoplastics in future applications. Thus, they offer promising prospects for wider utilization across various fields. In essence, this study provides valuable insights into the beneficial impact of MoS2 and PFA fillers on polyamide wear resistance.Highlights
The biobased poly (decamethylene terephthalamide) acid (PA10T) was synthesized through an efficient one‐pot melt polymerization method.
The composites with excellent performance were prepared by blending the biobased semi‐aromatic polyamide PA10T with unique fillers (MoS2 and PFA) for modification.
When 4 wt% PFA and 6 wt% MoS2 were added, the specific wear rate of composites was impressively reduced by 86.2% in comparison to pure PA10T.
The addition of fillers (MoS2 and PFA) not only improves the wear resistance of PA10T but also enhances its crystallization properties and melting points effectively.
The bending modulus of wear‐resistant composites increased to 1553.66 MPa, representing an approximately 50.47% increase compared to PA10T.
Microscopic dynamics and mechanism of Trichel pulse discharge
Authors: Jiang Xinchen , Wang Suikai , Ha Jing , Liu Fucheng , He Shoujie , Li Qing 
Published: January 2024

AbstractThe microscopic dynamics and formation mechanism of Trichel pulse discharge are investigated. A nonequal current transient effect exists in the Trichel pulse discharge. By reducing the limiting current resistance, the simulated current waveforms of Trichel pulse discharge are qualitatively and quantitatively consistent with those in the experiment. The dominant positive ions vary at different stages, which originate from the evolution of dominant reactions for the generation and consumption of charged particles. The existence of positive and negative charged layers results in the attenuation of the electric field at the rising edge of the Trichel pulse. The formation of the Trichel pulse originates from the changes in spatial charge density, resulting in alterations in the spatial electric field and ionization rate.