bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zika, chikungunya and co-occurrence in Brazil: space-time clusters and associated environmental–socioeconomic factors
Authors: Gardini Sanches Palasio Raquel , Marques Moralejo Bermudi Patricia , Luiz de Lima Macedo Fernando , Reis Santana Lidia Maria , Chiaravalloti-Neto Francisco 
Published: October 2023

AbstractChikungunya and Zika have been neglected as emerging diseases. This study aimed to analyze the space-time patterns of their occurrence and co-occurrence and their associated environmental and socioeconomic factors. Univariate (individually) and multivariate (co-occurrence) scans were analyzed for 608,388 and 162,992 cases of chikungunya and Zika, respectively. These occurred more frequently in the summer and autumn. The clusters with the highest risk were initially located in the northeast, dispersed to the central-west and coastal areas of São Paulo and Rio de Janeiro (2018–2021), and then increased in the northeast (2019–2021). Chikungunya and Zika demonstrated decreasing trends of 13% and 40%, respectively, whereas clusters showed an increasing trend of 85% and 57%, respectively. Clusters with a high co-occurrence risk have been identified in some regions of Brazil. High temperatures are associated with areas at a greater risk of these diseases. Chikungunya was associated with low precipitation levels, more urbanized environments, and places with greater social inequalities, whereas Zika was associated with high precipitation levels and low sewage network coverage. In conclusion, to optimize the surveillance and control of chikungunya and Zika, this study’s results revealed high-risk areas with increasing trends and priority months and the role of socioeconomic and environmental factors.
A single probe for solvent dependent optical recognition of iron(II/III) and arsenite: discrimination between iron redox states with single crystal X-ray structure evidence
Authors: Das Jayanta , Ghosh Milan , Ghosh Biplab , Mandal Prasenjit , Maji Sangita , Das Debasis 
Published: October 2023

AbstractThe detection and discrimination of Fe2+ and Fe3+ ions have been investigated using a simple probe (L), produced by the condensation of ethylenediamine and 3-ethoxysalicyaldehyde. Single crystal X-ray structures demonstrate that L interacts with Fe2+ and Fe3+. In aqueous-DMSO media, the L recognises AsO2− by fluorescence and colorimetry techniques. The AsO2− aided PET inhibition and H-bond assisted chelation enhanced fluorescence (CHEF) boost fluorescence by 91-fold. The L can detect 0.354 ppb Fe2+, 0.22 ppb Fe3+ and 0.235 ppt AsO2−.
Application of ensemble machine learning approach to assess the factors affecting size and polydispersity index of liposomal nanoparticles
Authors: Hoseini Benyamin , Jaafari Mahmoud Reza , Golabpour Amin , Momtazi-Borojeni Amir Abbas , Karimi Maryam , Eslami Saeid 
Published: October 2023

AbstractLiposome nanoparticles have emerged as promising drug delivery systems due to their unique properties. Assessing particle size and polydispersity index (PDI) is critical for evaluating the quality of these liposomal nanoparticles. However, optimizing these parameters in a laboratory setting is both costly and time-consuming. This study aimed to apply a machine learning technique to assess the impact of specific factors, including sonication time, extrusion temperature, and compositions, on the size and PDI of liposomal nanoparticles. Liposomal solutions were prepared and subjected to sonication with varying values for these parameters. Two compositions: (A) HSPC:DPPG:Chol:DSPE-mPEG2000 at 55:5:35:5 molar ratio and (B) HSPC:Chol:DSPE-mPEG2000 at 55:40:5 molar ratio, were made using remote loading method. Ensemble learning (EL), a machine learning technique, was employed using the Least-squares boosting (LSBoost) algorithm to accurately model the data. The dataset was randomly split into training and testing sets, with 70% allocated for training. The LSBoost algorithm achieved mean absolute errors of 1.652 and 0.0105 for modeling the size and PDI, respectively. Under conditions where the temperature was set at approximately 60 °C, our EL model predicted a minimum particle size of 116.53 nm for composition (A) with a sonication time of approximately 30 min. Similarly, for composition (B), the model predicted a minimum particle size of 129.97 nm with sonication times of approximately 30 or 55 min. In most instances, a PDI of less than 0.2 was achieved. These results highlight the significant impact of optimizing independent factors on the characteristics of liposomal nanoparticles and demonstrate the potential of EL as a decision support system for identifying the best liposomal formulation. We recommend further studies to explore the effects of other independent factors, such as lipid composition and surfactants, on liposomal nanoparticle characteristics.
Shedding light on the environmental impact of the decomposition of perovskite solar cell
Authors: Sabahi Negin , Shahroosvand Hashem 
Published: October 2023

AbstractPerovskite materials, as the heart of perovskite solar cells (PSC), attracted great interest in the photovoltaic community since the efficiency of PSC dramatically increased to over 25% in a short period. However, the presence of Pb metal in the perovskite crystalline limits the progress of this new generation of solar cells from environmental aspects. Here, we have systematically investigated the impact of the decomposition of perovskite material on the special plant, named Coleus. The influence of the decomposition of a perovskite solar cell (p-PbI2) has a three-fold lower destruction than commercial PbI2 (s-PbI2) in the same condition. The p-PbI2 made destroying the roots and leafs slower and smoother than s-PbI2, which the amount of water absorption with the plant’s root from p-PbI2 is two-fold lower than s-PbI2. The atomic absorption spectroscopy (AAS) indicated that the amount of Pb in the first week is about 3.2 and 2.1 ppm for s-PbI2, and p-PbI2, respectively, which in following for two next weeks reached to about relatively close together and finally in the last week decreased to 1.8 ppm for s-PbI2 and increased to 2.4 ppm for p-PbI2. This paper opens new avenues and challenges about the actual scenario on the impact of perovskite materials in PSCs on the plant and live metabolisms.
An analysis on history of childhood adversity, anxiety, and chronic pain in adulthood and the influence of inflammatory biomarker C-reactive protein
Authors: Dalechek Danielle E. , Caes Line , McIntosh Gwenne , Whittaker Anna C. 
Published: October 2023

AbstractDespite a link between adverse childhood experiences (ACEs) and anxiety, the role of anxiety in the pathway to chronic pain is unclear. Potentially, inflammatory biomarkers such as C-reactive protein (CRP) are involved. Objectives were to (1) examine relationships between reported ACEs, anxiety, and chronic pain, and (2) assess associations between ACEs, anxiety, and CRP levels and between CRP and chronic pain. Data from 24,172 adults who participated in the UK Biobank were used to conduct Poisson regressions to assess relationships between ACEs, anxiety, and chronic pain. For participants with CRP data who met the inclusion criteria (n = 2007), similar models were run between ACEs, anxiety, and CRP, and CRP and chronic pain. For objective 1, three statistically significant interactions were found to predict pain: frequency of physical abuse x reported muscular symptoms during anxiety (p = 0.01); frequency in which they felt hated x having discussed anxiety with a professional (p = 0.03), and reported frequency of sexual abuse x difficulties relaxing during anxiety attacks (p = 0.03). For objective 2, frequency of sexual abuse and informing a professional about anxiety significantly interacted to predict elevated CRP. For correlations, the largest was between CRP and the number of times pain was reported over the years (p = 0.01). Finally, ACEs (physical abuse, sexual abuse, and whether taken to a doctor) significantly interacted with CRP to predict pain. This study suggests mechanisms of the impact of ACEs on chronic pain may include inflammation and anxiety, which warrants further study.
Tau accumulation in degradative organelles is associated to lysosomal stress
Authors: Piovesana Ester , Magrin Claudia , Ciccaldo Matteo , Sola Martina , Bellotto Manolo , Molinari Maurizio , Papin Stéphanie , Paganetti Paolo 
Published: October 2023

AbstractNeurodegenerative disorders are characterized by the brain deposition of insoluble amyloidogenic proteins, such as α-synuclein or Tau, and the concomitant deterioration of cell functions such as the autophagy-lysosomal pathway (ALP). The ALP is involved in the degradation of intracellular macromolecules including protein aggregates. ALP dysfunction due to inherited defects in lysosomal or non-lysosomal proteins causes a group of diseases called lysosomal storage disorders (LSD) because of abnormal accumulation of lysosomal degradation substrates. Supporting the contribution of ALP defects in neurodegenerative diseases, deposition of amyloidogenic proteins occurs in LSD. Moreover, heterozygous mutations of several ALP genes represent risk factors for Parkinson’s disease. The reciprocal contribution of α-synuclein accumulation and lysosomal dysfunction have been extensively studied. However, whether this adverse crosstalk also embraces Tau pathology needs more investigation. Here, we show in human primary fibroblasts that Tau seeds isolated from the brain of Alzheimer’s disease induce Tau accumulation in acidic degradative organelles and lysosomal stress. Furthermore, inhibition of glucocerebrosidase, a lysosomal enzyme mutated in Gaucher’s disease and a main risk for Parkinson’s disease, causes lysosomal dysfunction in primary fibroblasts and contributes to the accumulation of Tau. Considering the presence of Tau lesions in Parkinson’s disease as well as in multiple neurodegenerative disorders including Alzheimer’s disease, our data call for further studies on strategies to alleviate ALP dysfunction as new therapeutic opportunity for neurodegenerative diseases and LSD.
Spatial–temporal coupling coordination and interaction between digitalization and traditional industrial upgrading: a case study of the Yellow River Basin
Authors: Wang Manman , Zhu Xiaoya , Yin Shi 
Published: October 2023

AbstractThe realization of coupling coordination between digitalization and traditional industrial upgrading in the Yellow River Basin holds significant practical value for promoting high-quality industrial development in the region. In order to assess this coupling coordination, we utilized inter-provincial panel data from nine provinces in the Yellow River Basin, covering the period from 2011 to 2020. Through the application of a coupling coordination degree model, we calculated the degree of coupling coordination and relative development between digitalization and traditional industrial upgrading. Additionally, we conducted a spatial–temporal analysis to identify the characteristics and trends of digitalization and traditional industrial upgrading. Furthermore, we constructed a panel VAR model to examine the interactive relationship between these two factors. The findings are as follows: (1) overall, over the study period, the degree of coupling coordination between digitalization and traditional industrial upgrading in the Yellow River Basin transitioned from a disordered state to a run-in stage. The corresponding development type changed from a low steady state to a co-existence of low and medium steady states. Notably, the levels of digitalization, traditional industrial upgrading, and coupling coordination all exhibited a gradual increase, while the relative development degree declined. (2) The coupling coordination degree between digitalization and traditional industrial upgrading in the Yellow River Basin demonstrated significant regional variation. Provinces displaying a “high–high” agglomeration distribution and “low–low” agglomeration distribution were concentrated in the middle and lower reaches, as well as the upper reaches, of the Yellow River. Furthermore, there was a positive spatial autocorrelation between these regions. (3) Both digitalization and traditional industrial upgrading exhibit self-reinforcing mechanisms, and a long-term dynamic correlation exists between them.
Efficacy of various extracting solvents on phytochemical composition, and biological properties of Mentha longifolia L. leaf extracts
Authors: Tourabi Meryem , Metouekel Amira , ghouizi Asmae E. L. , Jeddi Mohamed , Nouioura Ghizlane , Laaroussi Hassan , Hosen Md. Eram , Benbrahim Kawtar Fikri , Bourhia Mohammed , Salamatullah Ahmad Mohammad , Nafidi Hiba-Allah , Wondmie Gezahign Fentahun , Lyoussi Badiaa , Derwich Elhoussine 
Published: October 2023

AbstractThe current work attempts to explore the influence of three extraction solvents on phytochemical composition, content of polyphenols, antioxidant potential, and antibacterial capacity of hydroethanolic, acetonic, and aqueous extracts from Moroccan Mentha longifolia leaves. To achieve this goal, the chemical composition was identified using an HPLC–DAD examination. The contents of polyphenols were assessed, while the total antioxidant capacity (TAC), the DPPH test, and the reducing power test (RP) were utilized to determine antioxidant capacity. To assess the antibacterial activity, the microdilution technique was carried out to calculate the minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) of extracts against four nosocomial bacteria (Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus). Additionally, the antibacterial and antioxidant activities of all tested extracts were examined in silico against the proteins NADPH oxidase and Bacillus cereus phospholipase C. Study reveals that M. longifolia extracts contain high phenolic and flavonoids. Additionally, the hydroethanolic extract contained the highest amounts of phenolic and flavonoid content, with values of 23.52 ± 0.14 mg Gallic acid equivalent/g dry weight and 17.62 ± 0.36 mg Quercetin Equivalent/g dry weight, respectively compared to the other two extracts. The same extract showed the best antioxidant capacity (IC50 = 39 µg/mL ± 0.00), and the higher RP (EC50 of 0.261 ± 0.00 mg/mL), compared to the acetonic and aqueous extract regarding these tests. Furthermore, the hydroethanolic and acetonic extracts expressed the highest TAC (74.40 ± 1.34, and 52.40 ± 0.20 mg EAA/g DW respectively), compared with the aqueous extract. Regarding antibacterial activity, the MIC value ranges between 1.17 and 12.50 mg/mL. The in-silico results showed that the antibacterial activity of all extracts is principally attributed to kaempferol and ferulic acid, while antioxidant capacity is attributed to ferulic acid.
An improved approach of totally visceral sac separation (TVS) for incisional hernia compared with laparoscopic intraperitoneal onlay mesh plus repair (IPOM plus)
Authors: Zhuang Bo , Zheng Lushan , Yu Shian , Li Gang 
Published: October 2023

AbstractEndoscopic techniques have been widely used in ventral hernia surgery. Totally visceral sac separation (TVS) is a new concept proposed for hernia repair in recent years. The aim of this study was to contrast the postoperative results of TVS with the widely used method of Laparoscopic intraperitoneal onlay mesh plus repair (IPOM plus) for incisional hernias. The retrospective comparison analysis of 38 IPOM plus and 34 TVS was conducted during the time period between December 2019 and June 2022. For both two groups, baseline characteristics, surgical records, postoperative information, and quality of life outcomes utilizing the Carolina’s Comfort Scale were collected and analyzed. There were no differences between the methods of TVS and IPOM plus among the baseline characteristics. It showed the operative time in TVS group with the mean time of 213.4 min was significantly longer than that in IPOM plus group with the mean time of 182.9 min (P = 0.010). The postoperative length of stay in TVS group was 6.2 days, which was significantly shorter than IPOM plus group with the mean time of 4.8 days (P = 0.011). The medical expenses was significantly smaller in TVS group than that in IPOM plus group (P < 0.001). The quality of life scores of TVS were significant better than IPOM plus at one week, one month and six months. Besides, both TVS and IPOM plus have very few complications. TVS approach for incisional hernias is secure, effective, and valuable. It has shorter postoperative length of stay, higher quality of life, longer operative time, smaller medical expenses, and approximate complications compared with IPOM plus procedure. Our results have a greater contribution to the application and popularization of TVS technique.
Shrimp (Penaeus vannamei) survive white spot syndrome virus infection by behavioral fever
Authors: Rakhshaninejad Mostafa , Zheng Liping , Nauwynck Hans 
Published: October 2023

AbstractBoth endotherms and ectotherms may raise their body temperature to limit pathogen infection. Endotherms do this by increasing their basal metabolism; this is called ‘fever’. Ectotherms do this by migrating to warmer places; this is called ‘behavioral fever’. White spot syndrome virus (WSSV) is the most lethal pathogen of cultured shrimp. This study examined the existence of behavioral fever in WSSV-infected Penaeus vannamei shrimp. Shrimp weighing 15 ± 0.5 g were inoculated intramuscularly with WSSV and kept in a four-compartment system (4-CS) with all the chambers at 27 °C or with a thermal gradient (27–29–31–33 °C). During the first 4 days post-inoculation, 94% of the WSSV-inoculated shrimp died in the 4-CS with a fixed temperature (27 °C), while only 28% died in the 4-CS with a temperature gradient. The inoculated animals clearly demonstrated a movement towards the warmer compartments, whereas this was not the case with the mock- and non-inoculated animals. With primary lymphoid organ cell cultures, it was demonstrated that the increase of temperature from 27–29 °C to 31–33 °C inhibits virus replication. It is concluded that behavioral fever is used by shrimp to elevate their temperature when infected with WSSV. Behavioral fever prevents WSSV infection and mortality.
Assessment of expression of calcium signaling related lncRNAs in epilepsy
Authors: Taheri Mohammad , Pourtavakoli Ashkan , Eslami Solat , Ghafouri-Fard Soudeh , Sayad Arezou 
Published: October 2023

AbstractCalcium signaling is a metabolic pathway that is essential in neurons development and can be involved in the pathobiology of epilepsy. We assessed expression of three mRNA coding gene (SLC1A1,SLC25A12, andATP2B2) and three related long non-coding RNAs (LINC01231:1,lnc-SLC25A12-8:1and lnc-MTR-1:1) from this pathway in 39 patients with refractory epilepsy and 71 healthy controls. Expression of all genes except for lnc-SLC25A12 was higher in total epileptic cases compared with controls (P values = 0.0002, < 0.0001, < 0.0001, 0.049 and 0.0005 for SLC1A1, SLC25A12, LINC01231, ATP2B2 and lnc-MTR-1, respectively. When we separately compared expression of genes among males and females, SLC1A1, SLC25A12, LINC01231 and lnc-MTR-1 showed up-regulation in male cases compared with male controls. Moreover, expressions of SLC1A1 and SLC25A12 were higher in female cases compared with female controls. Remarkably, SLC25A12 was found to have the highest sensitivity value (= 1) for differentiation of epileptic cases from controls. Moreover, lnc-MTR-1 and lnc-SLC25A12 were sensitive markers for such purpose (sensitivity values = 0.89 and 0.87, respectively). The highest value belonged to LINC01231 with the value of 0.76. Taken together, this study demonstrates dysregulation of calcium-signaling related genes in epileptic patients and suggests these genes as potential biomarkers for epilepsy.
Simulation and optimization of the impacts of metal-organic frameworks on the hydrogen adsorption using computational fluid dynamics and artificial neural networks
Authors: Pourrahmani Hossein , Mohammadi Mohammad Hadi , Pourhasani Bahar , Gharehghani Ayat , Moghimi Mahdi , Van herle Jan 
Published: October 2023

AbstractOne of the barriers to further commercialization of the proton exchange membrane fuel cell (PEMFC) is hydrogen storage. Conventional methods are based on pressurizing the hydrogen up to 700 bar. The focus of this study is to characterize the hydrogen storage capacity of hydrogen tanks filled with MOF-5 at low pressures. Thus, Computational Fluid Dynamic (CFD) was used in a transient condition to analyze the hydrogen storage. Benefiting from the CFD model, three input parameters of the MOF-5, namely, density, specific heat, and conductivity, were utilized to develop an artificial neural network (ANN) model to find the highest mass of adsorption at the lowest required pressure. The optimum possible MOF among 729220 different possibilities, which enables the adsorption of 0.0099 kg at 139 bar, was found using a newly defined parameter called Pressure Adsorption Parameter (PAP).
Psychological status of patients with functional anorectal pain and treatment efficacy of paroxetine in alleviating the symptoms: a retrospective study
Authors: Fan Mingfeng , Li Laian , Xu Xiangjun , Zhou Cong , Wang Peng , Yin Wanbin , Pei Wenju , Wang Shuai 
Published: October 2023

AbstractThe aim of this study was to investigate the clinical characteristics, psychological status, sleep quality, and quality of life of patients with functional anorectal pain (FAP). The study also assessed the treatment efficacy of paroxetine in alleviating FAP symptoms. A retrospective comparative study of forty-three patients with FAP who were first treated with an anal plug compound glycolate suppository versus paroxetine combined with anal plug compound glycolate suppository between November 2021 and August 2022. Pain, quality of life, depression, anxiety and sleep quality were assessed before and after treatment by the Chinese version of the Short-Form McGill Pain Questionnaire-2 (SF-MPQ-2), Health-related quality of life scale (The 12-Item Short-Form Health Survey, SF-12), 17-item Hamilton Depression Rating Scale (HDRS), 14-item Hamilton Anxiety Scale (HAMA), and Pittsburgh Sleep Quality Index (PSQI). A total of 46.5% of patients with FAP were found to have anxiety symptoms (HAMA ≥ 7), 37.2% of patients with FAP were found to have depressive symptoms (HDRS ≥ 8). A total of 32.6% of patients with FAP had sleep disorders (PSQI > 10). Within 1 week after drug withdrawal, the short-term efficacy rate of oral paroxetine was 95.5%. After treatment, the symptom pain score (VAS) and sleep score were lower than those before treatment (P < 0.01). In the areas of vitality (VT), Social Functioning (SF), and Mental Health (MH), the difference between the pre-treatment and 8 weeks posttreatment scores of the study group and the control group was statistically significant (P < 0.05). FAP patients have obvious symptoms of anxiety and depression, and the incidence of sleep disturbance is prevalent. Paroxetine, a typical serotonin reuptake inhibitor (SSRI), was able to alleviate depression, anxiety, and pain symptoms in FAP, which might have clinical application prospects.