bluuebunny/crossref_metadata_2025_split huggingface dataset:
Radiologically guided percutaneous fine‐needle aspiration biopsy of the liver: Retrospective study of 119 cases evaluating diagnostic effectiveness and clinical complications
Authors: Guo Ziwen , Kurtycz Daniel F.I. , Salem Riad , De Las Casas Luis E. , Caya James G. , Hoerl H. Daniel 
Published: August 2002

AbstractWe reviewed 119 percutaneous, radiologically guided fine‐needle aspirations (FNA) from 114 patients with liver masses to evaluate diagnostic effectiveness and complications of this procedure. Satisfactory material was obtained in 118 cases (99%), of which 78 were diagnosed as positive (66%), three suspicious (2%), five atypical (4%), and 32 (27%) as negative for malignancy. Compared to surgical biopsy (48 cases) and clinical data, the sensitivity and specificity of FNA for malignancy was 95.1% and 100%, respectively, yielding a positive predictive value of 100% and a negative predictive value of 88.8%. Four cytology cases (3.4%) were false‐negatives (FN); all were interpretive errors. Four FN surgical biopsies (8.3%) were sampling errors. Minor complications occurred in three cases (2.5%). We conclude that FNA is safe and effective for determining the malignant potential of liver masses and should be the procedure of choice. Our experience suggests that having a pathologist present in the radiology suite provides optimal patient care. Diagn. Cytopathol. 2002;26:283–289. © 2002 Wiley‐Liss, Inc.
Changing clinical course of patients with malignant mesothelioma: Implications for FNA cytology and utility of immunocytochemical staining
Authors: Yu Gordon H. , Soma Lori , Hahn Stephen , Friedberg Joseph S. 
Published: August 2002

AbstractThe diagnosis of both local recurrences and distant metastases of mesothelioma can be accomplished by fine‐needle aspiration (FNA) biopsy. Although the previous history of mesothelioma provides strong support for recurrent/metastatic mesothelioma, other diagnostic possibilities (particularly adenocarcinoma) may require exclusion via special stains in some cases. In this study, we report on the morphologic findings in 13 cases of mesothelioma which underwent FNA (7 metastatic lesions, 6 local recurrences). In addition, immunohistochemical staining results for 7 cases with available material using antibodies directed against cytokeratin AE 1/3 and two antibodies reported to show consistently positive results in mesothelioma (calretinin and cytokeratin 5/6) are reported and compared to results seen for 10 cases of adenocarcinoma. All cases of mesothelioma and adenocarcinoma showed strong staining with cytokeratin AE 1/3. Three of 7 cases of mesothelioma showed strong staining with calretinin, while only focal staining was detected in 3 additional cases; only one case showed positive staining with cytokeratin 5/6. One of 10 cases of adenocarcinoma showed calretinin positivity; however, at least focal staining with cytokeratin 5/6 was seen in 4 cases. These results suggest that cytokeratin 5/6 is neither a sensitive nor specific stain for the diagnosis of mesothelioma in cytology material. Calretinin appears to be more specific for mesothelioma but showed disappointing sensitivity for this tumor, potentially limiting its diagnostic utility in FNA material. Diagn. Cytopathol. 24:322–327, 2001. © 2001 Wiley‐Liss, Inc.
Spindle‐cell lesions of the liver: Diagnosis by fine‐needle aspiration biopsy
Authors: Guy Cynthia D. , Yuan Shan , Ballo Michael S. 
Published: August 2002

AbstractRarely, spindle‐cell lesions in liver fine‐needle aspiration biopsies (FNABs) are encountered. A retrospective review of our experience with lesions that are mesenchymal in origin or appearance was undertaken to elucidate the frequency and spectrum of these lesions. Image‐guided liver FNABs performed over a 3‐year period (n = 585) at our institution (1996–1998) were retrospectively evaluated. Cytologic smears, cell block preparations, and clinical follow‐up of lesions with spindle‐cell morphology were reviewed. Twenty‐nine of 585 cases were of spindle‐cell morphology (5%). Hemangiomas (n = 12, 41%) and metastatic sarcomas (n = 6, 21%) comprised the largest categories, followed by granulomatous inflammation (n = 3, 10%). Other cases included primary angiosarcoma and fibrolamellar hepatocellular carcinoma. The most frequent spindle‐cell liver lesion encountered is hemangioma, followed by metastatic leiomyosarcoma and granulomatous hepatitis. Awareness of diagnostic possibilities, special attention to specimen adequacy, and use of ancillary procedures can maximize diagnostic yield. Diagn. Cytopathol. 2001;25:94–100. © 2001 Wiley‐Liss, Inc.
Primary malignant melanoma of the uterine cervix: Report of a case diagnosed by cervical scrape cytology and review of the literature
Authors: Deshpande Archana H. , Munshi Maitreyee M. 
Published: August 2002

AbstractCervical melanoma is a rare neoplasm and is seldom diagnosed by cervical scrape cytology. The possibility of metastatic melanoma should be excluded before making a diagnosis of primary cervical melanoma. We present a case of primary cervical melanoma diagnosed by cervical scrape cytology and a review of literature. The patient presented with vaginal bleeding of 3 mo duration and an ulcerated cervical growth. Cervical smear showed bizarre and abnormal cells containing pigment. Diagn. Cytopathol. 2001;25:108–111. © 2001 Wiley‐Liss, Inc.
Lymph node cytology in Wegener's granulomatosis
Authors: Banerjee Anindo K. , Tungekar Muhammad F. , Derias Nawal 
Published: August 2002

AbstractWe report on cytological findings on aspirates from the cervical lymph node from a case of Wegener's granulomatosis (WG). The diagnostic utility of technique in diagnosing a sizable minority of WG patients who present with cervical lymphadenopathy is discussed. We outline an approach to diagnosis of necrotising granulomatous lesions in aspirates from lymph nodes in a tabular form. Diagn. Cytopathol. 2001;25:112–114. © 2001 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of pancreatoblastoma
Authors: Henke Andrew C. , Kelley Carolyn M. , Jensen Chris S. , Timmerman Timothy G. 
Published: August 2002

AbstractPancreatoblastoma is a rare pancreatic neoplasm seen most commonly in the pediatric age group. We report on the aspiration cytology and immunohistochemical findings of a pancreatoblastoma in a 16‐yr‐old male. Diagn. Cytopathol. 2001;25:118–121. © 2001 Wiley‐Liss, Inc.
Cytologic features of disseminated bacillus Calmette‐Guérin (BCG) infection
Authors: Cohen Marta , Drut Ricardo 
Published: August 2002

AbstractDisseminated bacillus Calmette‐Guérin (BCG) infection is an unusual complication of immunization against Mycobacterium tuberculosis with the bacillus Calmette‐Guérin. We report on 4 such cases in which the diagnosis was suspected at the fine‐needle aspiration biopsy (FNAB) procedure. Participants were 4 males (mean age, 21.5 mo; range, 8–36 mo) in good general condition, in whom epidemiology data favoring tuberculosis and presence of pulmonary tuberculosis were lacking. Cases 1 and 2 presented with a deep‐seated subcutaneous nodule located near the left mamilla and lower aspect of the left scapula, respectively, resulting from lymph node involvement by BCG. Cases 3 and 4 presented as an osteolytic lesion of the ninth right rib and right iliac bone, respectively. FNAB findings showed poorly to moderately cellular smears. Epithelioid histiocytes in a granuloma pattern with occasional multinucleated Langerhans‐type giant cells, lymphocytes, and polymorphonuclear leukocytes in a finely granular background with necrotic debris were found in all cases. The presence of isolated calcified spherules interspersed among the cells was found to be a useful finding for diagnosis. When dealing with disseminated BCG infections, clinical and cytological pictures must be evaluated as a whole in order to arrive at an accurate diagnosis. Diagn. Cytopathol. 2001;25:134–137. © 2001 Wiley‐Liss, Inc.
Pharmacodynamics and pharmacokinetics of YM128, a GPIIb/IIIa antagonist prodrug
Authors: Suzuki Ken‐ichi , Moritani Yumiko , Hisamichi Nami , Ichihara Masato , Akamatsu Seijiro , Arai Hiroshi , Matsushima Hiroshi , Nii Tomoko , Sato Kazuo , Taniuchi Yuta , Shigenaga Takeshi , Kaku Seiji , Kawasaki Tomihisa , Matsumoto Yuzo , Inagaki Osamu , Tomioka Kenichi , Yanagisawa Isao 
Published: August 2002

AbstractWe examined the biochemical properties of YM‐57029 ({4‐[4‐(4‐Carbamimidoylphenyl)‐3‐oxopiperazin‐1‐yl]piperidino}acetic acid monohydrochloride trihydrate) and the pharmacodynamics and pharmacokinetics of its prodrug, YM128 (Ethyl (Z)‐(4‐{4‐[4‐(N2‐hydroxycarbamimidoyl)phenyl]‐3‐oxopiperazin‐1‐yl}piperidino)acetate), an orally‐active glycoprotein IIb/IIIa (GPIIb/IIIa) antagonist. YM‐57029 strongly inhibited aggregation of human platelets induced by various agonists, with IC50 values ranging from 3.6 to 51 nM. YM‐57029 specifically inhibited fibrinogen binding to purified GPIIb/IIIa about 1,000‐fold more potently than Arg‐Gly‐Asp‐Ser (RGDS). Moreover, YM‐57029 effectively inhibited an Arg‐Gly‐Asp (RGD) peptide binding to platelets, suggesting that YM‐57029 competed with the RGD sequence of ligand. YM‐57029 or YM128 dose‐dependently inhibited ex vivo platelet aggregation after iv bolus injection or oral administration to beagle dogs and cynomolgus monkeys. However, YM128 exerted more potent and prolonged inhibitory effects on platelet aggregation than YM‐57029 after oral administration to cynomolgus monkeys. Furthermore, YM‐57029 prolonged template bleeding time at a dose that inhibited ex vivo platelet aggregation during cumulative iv infusion to cynomolgus monkeys. Metabolic and pharmacokinetic studies showed that YM128 effectively converted into YM‐57029 in liver microsomes from humans as well as dogs and monkeys, and that bioavailabilities of YM128 in dogs and monkeys were 32.3 and 22.2%, respectively. These results suggest that YM128, a prodrug of YM‐57029, may be a valuable GPIIb/IIIa antagonist with good bioavailability in humans. Drug Dev. Res. 55:149–161, 2002. © 2002 Wiley‐Liss, Inc.
Dipyrone inhibits ethanol withdrawal and pentylenetetrazol‐induced seizures in rats
Authors: Ergün Hakan , Uzbay İ Tayfun , Çelik Turgay , Kayir Hakan , Yeşilyurt Özgür , Tulunay F. Cankat 
Published: August 2002

AbstractThe effects of dipyrone, a nonnarcotic analgesic drug, on ethanol withdrawal and pentylenetetrazol (PTZ)‐induced seizures in male Wistar rats were investigated. Ethanol (7.2%) was given to rats by a liquid diet for 21 days. After 6 h of ethanol withdrawal, rats were exposed to an audiogenic stimulus (100 dB) for 60 sec for induction of seizures. Dipyrone (50, 100, and 200 mg/kg ip) and saline were administered to rats 30 min before audiogenic stimulus, and the effects of dipyrone on the seizures induced by audiogenic stimulus were evaluated. The effects of dipyrone on PTZ‐induced seizures were also evaluated in other individual groups of the rats. The same doses of dipyrone were injected in the rats ip 30 min before PTZ (50 mg/kg) injections. Immediately after PTZ injections, onset time and severity of the convulsions were recorded. Dipyrone significantly and dose‐dependently reduced both incidence and intensity of the ethanol withdrawal‐associated audiogenic seizures. It also inhibited significantly and dose‐dependently intensity but not onset time of the PTZ‐induced seizures. Dipyrone (50–200 mg/kg) did not produce any significant change in locomotor activity in naive rats. Our results suggest that dipyrone has some prominent anticonvulsant activities on both ethanol withdrawal and PTZ‐induced seizures in rats, and that using dipyrone may be a new and effective therapeutic approach in the treatment of seizures. Drug Dev. Res. 53:254–259, 2001. © 2001 Wiley‐Liss, Inc.
Role of COX‐1‐ and COX‐2‐synthesized prostaglandins in a rat model of arthritic pain
Authors: Martínez Rosa Ventura , Díaz M. Irene , Hernández G. Patricia , Ramírez Adriana M. Domínguez , Salazar Luis A. , Cabré Francesc , López‐Muñoz Francisco J. 
Published: August 2002

AbstractAn appropriate animal model for investigation of antiinflammatory analgesic effects of NSAIDs is the pain‐induced functional impairment model in the rat (PIFIR model). The PIFIR model provides a model of inflammatory and chronic pain similar to that of clinical gout. We investigated the possible role of peripheral prostaglandins synthesized by COX‐1 and COX‐2 in arthritic pain produced by uric acid in the rat PIFIR model. For this purpose, the antinociceptive effects of indomethacin, a nonselective COX‐1/COX‐2 inhibitor, and those of SC‐560 and MK‐966 (Vioxx), selective inhibitors of COX‐1 and COX‐2, respectively, on the functional impairment induced by intraarticular (i.a.) injection of uric acid into the knee joint of the right hindlimb were compared. The antinociceptive efficacy was determined from the area under the curve (AUC) values obtained from the time course of the antinociceptive effect. Animals received vehicle, 100 μg indomethacin, 100 μg SC‐560, or 50 μg of MK‐966 (these drug doses were selected from dose–response curves of each drug in previous experiments) 20 min before uric acid and the ability of the rat to use the injured hindlimb was recorded with a computerized system. The effect produced by each treatment was compared with that observed after the compounds were administered in the left hindlimb, i.e., contralateral administration. The results showed that all three compounds significantly inhibited uric acid‐induced dysfunction of the right hindlimb (control = 64 ± 8 au; indomethacin = 391 ± 30 au; MK‐966 = 368 ± 17 au; SC‐560 = 445 ± 25 au, ANOVA, Dunnett’s test, P < 0.01). In addition, the results using contralateral administration suggest that the analgesic effect was due to inhibition of locally produced COX‐1 and COX‐2 and, therefore, that no central mechanisms were involved. Taken together, the present data using the pain‐induced functional impairment model in rats support the idea that both COX isoforms (1 and 2) contribute to the local inflammatory response in the model and that they may have a role in the maintenance of physiological homeostasis. They might also suggest that the therapeutic benefits of NSAIDs are mainly due to inhibition of both COX isoforms. Drug Dev. Res. 51:253–259, 2000. © 2001 Wiley‐Liss, Inc.
The interaction of age and unconditioned stimulus intensity on long‐trace conditioned flavor aversion in rats
Authors: Misanin James R. , Goodhart Mark G. , Anderson Matthew J. , Hinderliter Charles F. 
Published: August 2002

AbstractTo see if the neural representation of the conditioned stimulus (CS) is available to old‐age rats beyond the time it is available to young adults, the intensity of the unconditioned stimulus (US) and the length of the CS–US interval were systematically varied in a trace conditioning experiment. Results indicated that increasing US intensity extends the interval over which trace conditioning is evident in old‐age rats but not in young adults, suggesting that trace decay occurs more rapidly in young rats. Results were interpreted in terms of age differences in the workings of hypothesized biochemical timing mechanisms that may directly influence the ability to associate stimuli over trace intervals in conditioned taste‐aversion procedures. © 2002 Wiley Periodicals, Inc. Dev Psychobiol 40: 131–137, 2002. DOI 10.1002/dev.10018
Effects of the uterine environment and neocortical ectopias upon behavior of BXSB‐Yaa+mice
Authors: Denenberg Victor H. , Hoplight Blair , Sherman Gordon F. , Mobraaten L. E. 
Published: August 2002

AbstractBetween 40–60% of BXSB‐Yaa+mice have neocortical ectopias, a genetically based brain anomaly. The presence of ectopias is known to affect several cognitive measures. A second way to affect cognition is by transferring embryos into foreign uteri. These variables were jointly investigated in three experiments. BXSB‐Yaa+mouse embryos were transferred into same‐strain uteri, or into uteri of hybrid mice. At birth, pups were cross‐fostered to hybrid mothers or were reared by their birth mothers. When adult, the mice were given a series of behavioral tests with primary emphasis upon cognitive competence. Across all three studies, mice transferred into hybrid uteri were superior in the Morris maze and the Lashley III maze, and performed more effectively in shuttlebox avoidance learning. They were less effective in the simple water escape task, and the uterus groups did not differ in discrimination learning. Thus, development within a foreign uterus enhanced spatial learning and fear‐based conditioning. Ectopic mice were superior to non‐ectopics in learning the Morris maze, a finding consistent with prior research using the congenic BXSB‐Yaa strain. There were Uterus × Ectopia interactions on a few measures, indicating that, under certain condition, whether the presence of ectopias is beneficial or detrimental is contingent upon the uterine environment within which the organism develops. © 2001 John Wiley & Sons, Inc. Dev Psychobiol 38: 154–163, 2001
Formation and maintenance of distinctive cell patterns by coexpression of membrane‐bound ligands and their receptors
Authors: Honda Hisao , Mochizuki Atsushi 
Published: August 2002

AbstractWe show that graded or checkerboard‐like cell patterns, and segmental domains along a body axis, can be generated by cell behaviors involving differences in intercellular repulsion. A membrane‐bound signal transduction system mediating contact‐dependent cell interactions includes membrane‐bound ligands (ephrins) and their receptors with tyrosine‐kinase activity (Eph proteins). These molecules mediate both repulsive and attractive interactions under bilateral threshold control, i.e., cells expressing the receptors adhere to a surface bearing a critical density of ligand reciprocal to the density of receptor but are repelled by a surface with other densities of ligand (Honda [1998] J. Theor. Biol. 192:235–246). We extend this model. General membrane‐bound ligands (not always ephrins) and their receptors are presumably coexpressed in a single cell under bilateral threshold control. Computer simulations of cell pattern formation showed that when coexpression of the ligand and receptor is reciprocal, the cells self‐organize into a pattern of segmental domains or a graded cell arrangement along the body axis. The latter process interprets positional information in terms of protein molecules. When coexpression of the two species of molecules is not always reciprocal, the cells generate various patterns including checkerboard and kagome (star) patterns. The case of separate expression of ligands and receptors in different cells is also examined. The mechanism of differences in cell repulsion is compared with the differential cell adhesion hypothesis, which has been used to explain cell sorting. © 2002 Wiley‐Liss, Inc.
Postnatal Msx1 expression pattern in craniofacial, axial, and appendicular skeleton of transgenic mice from the first week until the second year
Authors: Orestes‐Cardoso Silvana Maria , Nefussi Jean Raphael , Hotton Dominique , Mesbah Mohand , Orestes‐Cardoso Maria Do Socorro , Robert Benoît , Berdal Ariane 
Published: August 2002

AbstractPhenotypes associated with Msx1 mutations have established the prominent role of this divergent homeogene in skeletal patterning. Previous studies have been achieved during antenatal development in relation with the early death of null mutant mice. Therefore, the present study is devoted to Msx1 homeogene in the postnatal craniofacial, axial, and appendicular skeleton. A knock‐in transgenic mouse line was studied from the first postnatal week until 15 months. Whole‐mount β‐galactosidase enzymology identified Msx1 protein expression pattern. Maintained expression of Msx1 was observed in growing and adult mice, specifically in the sites where Msx1 plays an early morphogenetic role during initial skeletal patterning. These included the craniofacial sutures, autopodium, mandible, and alveolar bone. Furthermore, active membranous and endochondral bone formation involved Msx1 in the entire skeleton. Histologic sections showed that progenitor as well as differentiating and differentiated cells of all the bone cell lineages could express the Msx1 protein (chondrocytes, osteoblasts, tartrate‐resistant acid phosphatase positive osteoclasts and chondroclasts). Recent developments in the genetic and developmental biology of skeletal morphogenesis demonstrate that genes critical for development are jointly expressed in discrete embryonic signalling and growth centers, the enamel knot in teeth, the cranial suture in skull morphogenesis, and the progress zone in the limb buds. The present study suggests that these signalling pathways are jointly important throughout the entire lifetime with an exquisite site‐specificity spatially related to early patterning. © 2001 Wiley‐Liss, Inc.
Investigation of Prx1 protein expression provides evidence for conservation of cardiac‐specific posttranscriptional regulation in vertebrates
Authors: Chesterman Elizabeth S. , Gainey Georgina D. , Varn Allyson C. , Peterson Richard E. , Kern Michael J. 
Published: August 2002

AbstractGene targeting experiments have defined that the homeobox gene Prx1 is essential for normal craniofacial, limb, and vascular development. Although its RNA expression pattern is well established, Prx1 protein expression in the developing embryo has not been examined. A novel Prx1 antibody was produced to define the normal Prx1 protein expression pattern in the developing mouse embryo. In craniofacial and limb mesenchyme, Prx1 protein expression is consistent with previously published data on RNA localization. However, a remarkable discrepancy was found in cardiac tissue. Prx1 protein is undetectable in the murine embryonic and adult heart, despite the presence of Prx1 transcripts. These data demonstrate that Prx1 expression is posttranscriptionally regulated. This discrepancy between the presence of Prx1 transcript and the absence of detectable protein was also observed in embryonic chick heart, suggesting conservation of the regulatory mechanism in vertebrates. This observation provides a new explanation of why the Prx null mice lack cardiac malformations. © 2001 Wiley‐Liss, Inc.
Identification, cloning, and developmental expression of hepatoma‐derived growth factor in the developing rat heart
Authors: Everett Allen D. 
Published: August 2002

AbstractHepatoma derived growth factor (HDGF) was identified as a developmentally regulated cardiac gene by mRNA differential display using 12‐day rat fetal conotruncus vs. newborn aorta. The full‐length rat HDGF cDNA was cloned from a rat fetal heart cDNA library and found to be 94 and 88% homologous to the mouse and human sequence, respectively. The rat sequence, like the human and mouse, contains a highly conserved amino portion and putative bipartite nuclear localization sequence. By Northern analysis, HDGF is highly expressed in the fetal conotruncus, heart, kidney, brain, and gut. By immunocytochemistry, HDGF was first detected only in atrial myocytes, hind gut epithelia, and notochord of the E10 rat with a nuclear expression pattern. By E12, expression had broadened to include the ventricular myocytes, endocardial cells, and cells of the ventricular outflow tract. HDGF is unique in that it is the first described nuclear targeted growth factor in the developing heart. The early expression of HDGF in embryonic heart and fetal gut suggests that HDGF may play a role in cardiovascular growth and differentiation. © 2001 Wiley‐Liss, Inc.