bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of regucalcin as an activator of sarcoplasmic reticulum Ca2+‐ATPase activity in rat heart muscle
Authors: Yamaguchi Masayoshi , Nakajima Rie 
Published: October 2002

AbstractThe expression of regucalcin, a regulatory protein of Ca2+ signaling, and its effect on Ca2+ pump activity in the microsomes (sarcoplasmic reticulum) of rat heart muscle was investigated. The expression of regucalcin mRNA was demonstrated by reverse transcription‐polymerase chain reaction (RT‐PCR) analysis in heart muscle using rat regucalcin‐specific primers. Results with Western blot analysis showed that regucalcin protein was present in the cytoplasm, although it was not detected in the microsomes. Microsomal Ca2+‐ATPase activity was significantly increased in the presence of regucalcin (10−10–10−8 M) in the enzyme reaction mixture. This increase was not seen in the presence of thapsigargin (TP) (10−5 M), a specific inhibitor of the microsomal Ca2+ pump enzyme. Regucalcin (10−10–10−8 M) significantly stimulated ATP‐dependent 45Ca2+ uptake by the microsomes. The effect of regucalcin (10−8 M) in increasing microsomal Ca2+‐ATPase activity was completely prevented in the presence of digitonin (10−3 or 10−2%), which has a solubilizing effect on membranous lipid, or N‐ethylmaleimide (NEM), a modifying reagent of sulfhydryl (SH) groups. Dithiothreitol (DTT; 5 mM), a protecting reagent of SH groups, increased markedly Ca2+‐ATPase activity. In the presence of DTT (5 mM), regucalcin could not significantly enhance the enzyme activity. Also, the effect of regucalcin in increasing Ca2+‐ATPase activity was completely inhibited by the addition of vanadate (1 mM), an inhibitor of phosphorylation of enzyme. In addition, the effect of regucalcin on Ca2+‐ATPase activity was not significantly modulated in the presence of dibutyryl cyclic AMP (10−4 M), inositol 1,4,5‐trisphosphate (10−3 M), or calmodulin (5 μg/ml) which is an intracellular signaling factor. The present study demonstrates that regucalcin can activate Ca2+ pump activity in rat heart microsomes, and that the protein may act the SH groups of Ca2+‐ATPase by binding to microsomal membranes. J. Cell. Biochem. 86: 184–193, 2002. © 2002 Wiley‐Liss, Inc.
Specific induction of heat shock protein 27 by glucocorticoid in osteoblasts
Authors: Kozawa Osamu , Niwa Masayuki , Hatakeyama Daijiro , Tokuda Haruhiko , Oiso Yutaka , Matsuno Hiroyuki , Kato Kanefusa , Uematsu Toshihiko 
Published: October 2002

AbstractIt is generally recognized that osteoporosis is a common complication of patients with glucocorticoid excess and that glucocorticoid receptor is associated with heat shock protein (HSP) 70 and HSP90 in a heterocomplex. In the present study, we investigated whether glucocorticoid induces HSP27, HSP70, and HSP90 in osteoblast‐like MC3T3‐E1 cells. Dexamethasone time‐dependently increased the levels of HSP27, while having no effect on the levels of HSP70 or HSP90. The effect of dexamethasone was dose‐dependent in the range between 0.1 nM and 0.1 μM. Dexamethasone induced an increase of the levels of mRNA for HSP27. Dexamethasone induced the phosphorylation of p38 mitogen‐activated protein (MAP) kinase. SB203580 and PD169316, inhibitors of p38 MAP kinase, suppressed the HSP27 accumulation by dexamethasone. In addition, SB203580 reduced the dexamethasone‐stimulated increase of the mRNA levels for HSP27. The dexamethasone‐induced phosphorylation of p38 MAP kinase was reduced by SB203580. These results strongly suggest that glucocorticoid stimulates the induction of neither HSP70 nor HSP90, but HSP27 in osteoblasts, and that p38 MAP kinase is involved in the induction of HSP27. © 2002 Wiley‐Liss, Inc.
Detection and characterization of RANK ligand and osteoprotegerin in the thyroid gland
Authors: Hofbauer Lorenz C. , Kluger Sibylle , Kühne Christian A. , Dunstan Colin R. , Burchert Andreas , Schoppet Michael , Zielke Andreas , Heufelder Armin E. 
Published: October 2002

AbstractReceptor activator of NF‐κB (RANK) ligand (RANKL) and osteoprotegerin (OPG) play essential roles in bone metabolism and immune responses. RANKL activates RANK, which is expressed by osteoclasts and dendritic cells (DC), whereas OPG acts as its decoy receptor. The role of RANKL and OPG in thyroid physiology is unclear. Northern analysis revealed pronounced OPG mRNA levels in normal human thyroid. By contrast, RANKL mRNA levels were most abundant in lymph node and appendix, and low in the thyroid. In the human thyroid follicular cell line XTC and in primary human thyroid follicular cells, OPG mRNA levels and protein secretion were upregulated by interleukin (IL)‐1β ?(33‐fold), tumor necrosis factor (TNF)‐α (eightfold), and thyrotropin (TSH) (threefold). RANKL mRNA was stimulated in XTC by IL‐1β? and TNF‐α, but inhibited by TSH. Conditioned medium harvested from IL‐1β‐treated XTC (containing high concentrations of OPG) inhibited RANKL‐induced CD40 upregulation and cluster formation of DC. OPG mRNA levels were three times more abundant in surgical thyroid specimens of Graves' disease as compared to other thyroid diseases. Our data suggest that RANKL and OPG are produced in the thyroid gland by thyroid follicular cells, are regulated by cytokines and TSH, and are capable of modulating dendritic cell functions. Thus, these cytokines may represent important local immunoregulatory factors involved in the pathogenesis of autoimmune thyroid diseases. J. Cell. Biochem. 86: 642–650, 2002. © 2002 Wiley‐Liss, Inc.
Microwave exposure induces Hsp70 and confers protection against hypoxia in chick embryos
Authors: Shallom Joshua M. , Di Carlo Andrea L. , Ko Daejin , Penafiel L. Miguel , Nakai Akira , Litovitz Theodore A. 
Published: October 2002

AbstractTo determine if microwave exposure could elicit a biological effect in the absence of thermal stress, studies were designed in which chick embryos were exposed to athermal microwave radiation (915 MHz) to look for induction of Hsp70, a protein produced during times of cellular stress that aids in the protection of cellular components. Levels of Hsp70 were found to increase within 2 h, with maximum expression (∼30% higher than controls) typically occurring by 3 h from the start of exposure. Other embryos were exposed to microwave radiation prior to being subjected to hypoxic stress, and were found to have significantly higher survival (P < 0.05) following re‐oxygenation than non‐exposed controls. The results of these studies indicate that not only can athermal microwave exposures activate the stress protein response pathway; they can also enhance survivability following exposure to a subsequent, potentially lethal stress. From a public health standpoint, it is important that more studies be performed to determine if repeated exposures, a condition likely to be found in cell phone use, are still beneficial. J. Cell. Biochem. 86: 490–496, 2002. © 2002 Wiley‐Liss, Inc.
Interaction of the retinoblastoma gene product, RB, with cyclophilin A negatively affects cyclosporin‐inhibited NFAT signaling
Authors: Cui Yukun , Mirkia Kirash , Florence Fu Yu‐Hsieh , Zhu Li , Yokoyama Kazunari K. , Chiu Robert 
Published: October 2002

AbstractThe retinoblastoma susceptibility gene product, p105Rb (RB), is generally believed to be an important regulator in the control of cell growth, differentiation, and apoptosis. Several cellular factors that form complexes with RB and exert their cellular regulatory functions have been identified, such as the newly identified RB:cyclophilin A (CypA) complex. The physical interactions between RB and CypA were demonstrated by glutathione S‐transferase affinity matrix binding assays and immunoprecipitation, followed by Western blot analyses. The N‐terminal region of CypA mediated the interaction with RB, whereas the region upstream of the A‐pocket of RB was required for binding to CypA. Ectopic expression of RB into Jurkat cells partially blocks the function of cyclosporin (CsA) to inhibit nuclear factor for activation of T cell (NFAT) activation by phorbol ester (PMA) plus ionomycin A (IA), suggesting that RB may prevent CsA inhibition of T lymphocyte activation. These results are further evidenced by the effect of RB on both calcineurin (CN) and NFAT binding activity in vitro, suggesting that the interaction of RB with CypA interferes with the CsA:CypA complex and blocks CsA‐inhibited CN activity. These data reveal the functional link between RB and CypA and their involvement in T cell activation signaling. J. Cell. Biochem. 86: 630–641, 2002. © 2002 Wiley‐Liss, Inc.
Reducing I/O costs for the eigenvalue procedure in large‐scale configuration interaction calculations
Authors: Shepard Ron , Shavitt Isaiah , Lischka Hans 
Published: October 2002

AbstractSeveral aspects of the matrix diagonalization method used for CI calculations in the COLUMBUS Program System are discussed, including a linear basis‐contraction algorithm and the use of a nonorthogonal expansion basis. Both of these features significantly reduce the I/O requirements during the iterations. © 2002 Wiley Periodicals, Inc. J Comput Chem 23: 1121–1125, 2002
Studies on 4,7‐di‐substitution effects of one ligand in [Ru(Phen)3]2 with DFT method
Authors: Zheng Kangcheng , Wang Juping , Shen Yong , Peng Wenlie , Yun Fengcun 
Published: October 2002

AbstractStudies on the complex [Ru(phen)3]2+ (phen = 1,10‐phenanthroline) and its derivatives with 4,7‐di‐substitution on one ligand(phen) were carried out using the DFT method at the B3LYP/LanL2DZ level of theory. The trends in the substituent effects caused by the electron‐pushing group (OH) and the electron‐withdrawing group (F), on the electronic structures and the related properties, for example, the energies and the components of some frontier molecular orbitals, the spectroscopy properties, and the net charge populations of some main atoms of the complexes, etc., have been investigated. The computational results show that the substituents have some interesting effects on the electronic structures and the related properties of the complexes. First, according to the analysis of components of LUMO of the complexes, the electron‐withdrawing group (F) can activate the main ligand (the substituted ligand, i.e., 2R‐phen) and passivate the coligands, on the contrary, the electron‐pushing group (OH) can activate the coligands and passivate the main ligand in the first electronic excited states of complexes. Second, both the electron‐pushing group (OH) and the electron‐withdrawing group (F) can cause a red shift in the electronic ground bands. Third, the characteristics of the atomic net charge populations on the main ligand can also be analyzed in detail by means of a schematic map expressed by several series of arrowheads based on the law of polarity alternation and the idea of polarity interference. The most negative charges are populated on N1, the next most net negative charges are populated on C3 among the skeleton atoms for the three complexes, etc. The computational results can be better used to explain some experimental phenomena and trends. © 2002 Wiley Periodicals, Inc. J Comput Chem 4: 436–443, 2002; DOI 10.1002/jcc.10038
EQUIPATH—an equilibrial path tracing routine for the use with the program package GAUSSIAN94
Authors: Kliesch Wolfgang 
Published: October 2002

AbstractThe present article deals with the numerical details important for a successful implementation of the equilibrial path tracing procedure EQUIPATH. The results of an application to the RHF/6‐31G(d) potential energy surface of butane are reported and discussed. Sixteen saddle points and several valley‐ridge inflection points have been located. For the fragmentation C4H10→ C2H6+C2H4 two different reaction paths have been found. © 2001 John Wiley & Sons, Inc. J Comput Chem 22: 1801–1816, 2001
Symptoms of schizophrenia and neurocognitive test performance
Authors: Shean Glenn , Burnett Traverse , Eckman F. Scott 
Published: October 2002

AbstractSchizophrenic in‐patients were rated on factor‐derived symptom dimensions based on negative and positive symptom ratings. Participants were administered neuropsychological tests (digit span, digit vigilance, logical memory, and Trails A and B) and then instructed on how to improve performance on the tests. Follow‐up tests were administered shortly after coaching. Comparisons of pre‐ and post‐instruction test scores indicated that the patient group was able to improve significantly their performance on the tests after coaching. Symptom dimensions were predictive of improved post‐instruction test performance beyond the impact of the level of pre‐instruction scores. Symptoms of disorganization and negative symptoms were related to lack of ability to improve performance on one or more of the tests, and positive symptoms and premorbid adjustment were associated with improved post‐instruction performance. The symptom dimensions provide a potentially useful approach to reducing heterogeneity within samples of schizophrenic patients. © 2002 Wiley Periodicals, Inc. J Clin Psychol 58: 723‐731, 2002.
Correspondence between supervisors and trainees in their perception of supervision events
Authors: Reichelt Sissel , Skjerve Jan 
Published: October 2002

AbstractThe focus of this study is on how the participants in 16 supervisory dyads perceive the content and process in a supervisory session, and on the meaning they attach to supervisory events. A central issue is to what degree the participants in each dyad correspond in their perceptions and evaluation of supervisory events. Another question is how lack of correspondence affects the trainees' experience of satisfaction with the supervision. A majority of the dyads were rated low or moderate in correspondence, and it is discussed whether the influence of low correspondence on trainee satisfaction may be related to supervisory intentions and style characteristics. A main point in the discussion is whether role ambiguity may be related to obscure communication and reduced correspondence, and it is suggested that more attention should be paid to negotiating and renegotiating rules for the supervisory relationship. © 2002 Wiley Periodicals, Inc. J Clin Psychol 58: 759‐772, 2002.
Predictors of graduate student attitudes toward prescription privileges for psychologists
Authors: Luscher Kristen A. , Corbin William R. , Bernat Jeffrey A. , Calhoun Karen S. , McNair Lily D. 
Published: October 2002

AbstractThe issue of gaining prescription privileges and its potential impact on the field of clinical psychology has special relevance for graduate students. This study was designed to investigate clinical graduate students' attitudes toward prescription authority, identify salient variables that contribute to these attitudes, and ascertain preferred models of training. Only 42.5% of respondents personally desired to obtain prescription privilege, although 61.8% of respondents favored efforts of the American Psychological Association to acquire prescription authority. Proponents and opponents were compared on their ratings of positive and negative aspects of the debate. There was strong agreement that the training should not be predoctoral and that it should lead to board certification. The strongest predictors of graduate students' attitudes were concerns about fundamental change to the field, malpractice premiums, and whether they considered it a logical extension of the field. This study provides a framework for understanding important factors influencing the decision‐making process among clinical psychology graduate students. © 2002 Wiley Periodicals, Inc. J Clin Psychol 58: 783‐792, 2002.
Depression and trauma history in first‐time mothers receiving home visitation
Authors: Stevens Jack , Ammerman Robert T. , Putnam Frank G. , Van Ginkel Judith B. 
Published: October 2002

AbstractThis article examines the prevalence and correlates of depression and trauma history in first‐time mothers receiving home visitation. One hundred twenty‐three mothers in a Healthy Families America (HFA) program completed measures of depression, trauma history, social support, sense of control, and child abuse risk. Number of completed home visits, number of uncompleted home visits, and total telephone contact time were documented for each participant over the first 6 months of enrollment. Nearly 30% of the mothers scored in the clinically significant range on the depression measure. Approximately 70% of the sample had experienced at least one violent trauma. Depression and violent trauma, but not nonviolent trauma, were related to greater child abuse risk and less maternal sense of control and social support. Violent traumas were related to greater interruptions in service delivery as reflected by more uncompleted home visits. After controlling for social support and sense of control, depression was associated with more telephone contact between mothers and home visitors. Mothers receiving home visitation exhibited a heightened incidence of depression and trauma history relative to the population at large. These constructs were related to social support and sense of control, both of which are targets of home visitation. Depression and trauma history may significantly influence the delivery and outcomes obtained from home visitation. © 2002 Wiley Periodicals, Inc.
Progression elevated gene‐3, PEG‐3, induces genomic instability in rodent and human tumor cells
Authors: Su Zao‐zhong , Gopalkrishnan Rahul V. , Narayan Gopeshwar , Dent Paul , Fisher Paul B. 
Published: October 2002

AbstractGenomic instability is a fundamental component of cancer progression. Subtraction hybridization identified a novel rodent gene, progression elevated gene‐3 (PEG‐3) whose expression directly correlates with cancer aggressiveness and progression. Moreover, ectopic expression of PEG‐3 in rodent or human tumor cells produces an aggressive transformed phenotype. We demonstrate that PEG‐3 expression in rodent tumor cells correlates directly with genomic instability as characterized by alterations in chromosome composition and structure. Additionally, elevated endogenous or ectopic expression of PEG‐3 in rodent and human tumor cells, respectively, enhances gene amplification, as monitored by resistance to methothrexate (MTX) and amplification of the dihydrofolate reductase (dhfr) gene. Stable expression of PEG‐3 in normal cloned rat embryo fibroblast (CREF) cells marginally elevates MTX resistance, but morphology remains unaltered and anchorage independence is not induced, suggesting that these phenotypes are separable in immortal cells and gene amplification may precede the acquisition of morphological and oncogenic transformation. The present studies document that stable, inducible, and transient expression of PEG‐3 in cancer cells augments genomic instability. In these contexts, one mechanism by which PEG‐3 influences cancer progression may be by preferentially facilitating the development of genomic changes in evolving cancer cells. © 2002 Wiley‐Liss, Inc.
Observer experience improves reproducibility of color doppler sonography of orbital blood vessels
Authors: Németh János , Kovács Rita , Harkányi Zoltán , Knézy Krisztina , Sényi Katalin , Marsovszky István 
Published: October 2002

AbstractPurpose.The study investigated the reproducibility of orbital blood flow measurements with color Doppler imaging (CDI) at different stages of observer experience.Methods.The subjects were 31 healthy volunteers and 2 sequential groups of 25 glaucoma patients each. Repeated blood flow measurements (usually 3 sets) in orbital vessels (ophthalmic artery, short posterior ciliary arteries, central retinal artery, and central retinal vein) were performed by the same observer in a single session in each subject.Results.The parameters with the best reproducibility were the resistance index (mean coefficient of variation [COV], 3.3–8.8%), the peak systolic velocity (mean COV, 6.9–13.7%), the time‐averaged velocity (mean COV, 7.2–16.0%), and the systolic acceleration time (mean COV, 8.8–12.3%). The mean COV was greater (9.9–20.3%) for the other arterial flow parameters (end‐diastolic velocity and systolic acceleration) and for the venous flow velocities (maximum and minimum). The COVs of the parameters were improved by 20–40% as the observer became more experienced in ophthalmic CDI.Conclusions.We confirm the general reliability of CDI measurements in orbital vessels and show that observer experience improves reproducibility. It appears, however, that observer performance in these measurements is vessel specific. © 2002 Wiley Periodicals, Inc. J Clin Ultrasound 30:332–335, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI: 10.1002/jcu.10079
Intraurethral sonography and the test‐retest reliability of urethral sphincter measurements in women
Authors: Heit Michael 
Published: October 2002

AbstractPurpose.The purpose of this prospective study was to determine the test‐retest reliability of urethral sphincter morphologic measurements obtained with intraurethral sonography.Methods.The cross‐sectional urethral sphincter anatomy of 29 asymptomatic nulliparous women was studied in a blinded fashion. Each patient returned for a repeat examination on a different day. At the point of maximal rhabdosphincter thickness, the urethral diameter and circumference and the longitudinal smooth muscle and rhabdosphincter thickness, diameter, circumference, and area were measured using the ultrasound scanner's integrated software. For each measured variable, the reliability between patients was assessed with a paired t test. Intraclass correlation coefficients were calculated to assess the reliability of each intraurethral sonographic measurement obtained from the same patient.Results.On test‐retest analysis, the differences for each measured variable between patients were not statistically significant (p > 0.05). Of the measurements obtained from the same patient, however, longitudinal smooth muscle thickness (ρ = 0.44; p = 0.006), diameter (ρ = 0.49, p = 0.003), circumference (ρ = 0.49, p = 0.003), and area (ρ = 0.43; p = 0.009) were significantly correlated.Conclusions.The urethral longitudinal smooth muscle layer is the only structure that can be measured reliably using sonography for diagnostic use. Sonographic measurements of the rhabdosphincter may not be reliable because the outer portion of that structure lies outside the depth of penetration of a 12.5‐MHz transducer. © 2002 Wiley Periodicals, Inc. J Clin Ultrasound 30:349–355, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI: 10.1002/jcu.10082
Changes in macrophage morphology and prolonged cell viability following exposure to polyethylene particulate in vitro
Authors: Xing Shaomo , Waddell J.E. , Boynton E.L. 
Published: October 2002

AbstractThe interaction of macrophages and ultra‐high molecular weight polyethylene (PE) wear plays an important role in perpetuating chronic inflammation at the bone implant interface, leading to peri‐implant osteolysis and mechanical failure of the implant. A model to study the interaction of human mature macrophages with orthopaedic biomaterial wear has been previously developed. With the use of the model, in this study, the mature human monocyte‐derived macrophages (MDMs) were observed with light, fluorescent, and scanning electron microscopy (SEM), as well as transmission electron microscopy (TEM). The cell viability was investigated using calcein and ethidium staining. Following exposure to PE particulate, the morphology of the human MDMs was heterogeneous: rounded, flattened, and elongated. There was no morphological evidence of cytotoxicity or apoptosis. The MDM viability was not influenced by phagocytosis of PE particulate in a negative fashion. In fact, more prolonged cell viability was observed in the human MDMs exposed to PE particulate when compared to controls. Microsc. Res. Tech. 57:523–529, 2002. © 2002 Wiley‐Liss, Inc.