bluuebunny/crossref_metadata_2025_split huggingface dataset:
Involvement of the cellular prion protein in seeding and spreading of sarkosyl-derived fractions of Alzheimer’s disease inPrnpmutant mice and in the P301S transgenic tauopathy mice model
Authors: Sala-Jarque Julia , Gil Vanessa , Andrés-Benito Pol , Lidón Laia , Yanac-Huertas Renato Eduardo , López-León Clara F , Hernández Félix , Ávila Jesús , Lanciego José Luis , Soriano Jordi , Nuvolone Mario , Aguzzi Adriano , Gavín Rosalina , Ferrer Isidro , del Río José Antonio 
Published: January 2024

AbstractThe natural cellular prion protein is known to play several roles during development and adult brain. Far from its pathological roles in prionopathies, the non-pathogenic cellular prion protein has been described as a receptor for several amyloid in oligomeric and prefibrillar forms. For some amyloids, specific domains of the protein play a crucial role in modulating amyloid’s cellular uptake and seeding properties. In most studies, the functions and the role of putative amyloid receptors have been analyzed by using brain extracts derived from human neurodegenerative patients. Another strategy has been to modify the genetic dosage of the natural prion protein in genetic models of different diseases. In this study, we take advantage of both approaches to examine whether this protein plays a role in the seeding and spreading of pathogenic tau. Our results point to a role of the natural prion protein in the emergence of pathogenic tau in a mouse model overexpressing the mutation P301S of the human tau gene. In contrast, its role is minor when sarkosyl-derived brain samples of Alzheimer’s disease are used. In fact, our results indicate that the use of this type of sample is not adequate to determine the role of a putative receptor in tau seeding and spreading.
Capturing G protein-coupled receptors into native lipid-bilayer nanodiscs using new diisobutylene/maleic acid (DIBMA) copolymers
Authors: Chu Ci , Vargas Carolyn , Barbosa Maria Carolina , Sommerhage Simon , Schröder Gunnar F. , Keller Sandro , Etzkorn Manuel 
Published: January 2024

AbstractMany membrane proteins, including G-protein-coupled receptors (GPCRs), are susceptible to denaturation when extracted from their native membrane by detergents. Therefore, alternative methods have been developed, including amphiphilic copolymers that enable the direct extraction of functional membrane proteins along with their surrounding lipids, leading to the formation of native lipid-bilayer nanodiscs. Among these amphiphilic copolymers, styrene/maleic acid (SMA) and diisobutylene/maleic acid (DIBMA) polymers have been extensively studied and successfully utilized to isolate various types of membrane proteins, including GPCRs. Despite their many benefits, SMA and DIBMA polymers also have significant drawbacks that limit their application. Most notably, both SMA and DIBMA carry high negative charge densities, which can interfere with protein–protein and protein–lipid interactions through unspecific Coulombic attraction or repulsion. Herein, we describe a series of new amphiphilic copolymers derived from DIBMA via partial amidation of the carboxylate pendant groups with various biocompatible amines. The nanodisc-forming properties of the new polymers were assessed using model membranes as well as in the context of extracting the melanocortin 4 receptor (MC4R), a prototypical class A GPCR. While each new DIBMA variant displays distinct features that may be favorable for selected applications, we identified a new PEGylated DIBMA variant called mPEG4-DIBMA as particularly promising for the studied purpose. On the one hand, mPEG4-DIBMA abolishes unspecific interactions with the tested peptide ligand, a prerequisite for reliably characterizing GPCR–ligand interactions. On the other hand, mPEG4-DIBMA outperforms other polymers such as SMA and DIBMA by achieving higher extraction efficiencies of MC4R from Sf9 insect cell membranes. Thus, this new nanodisc-forming polymer combines two key advantages that are crucial for investigating GPCRs in a well-defined but still native lipid-bilayer environment, thus paving the way for manifold future applications.
The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation
Authors: Tóth Sarolta , Kaszás Diána , Sónyák János , Tőkés Anna-Mária , Padányi Rita , Papp Béla , Nagy Réka , Vörös Kinga , Csizmadia Tamás , Tordai Attila , Enyedi Ágnes 
Published: January 2024

AbstractLoss of epithelial cell polarity and tissue disorganization are hallmarks of carcinogenesis, in which Ca2+signaling plays a significant role. Here we demonstrate that the plasma membrane Ca2+pump PMCA4 (ATP2B4) is downregulated in luminal breast cancer, and this is associated with shorter relapse-free survival in patients with luminal A and B1 subtype tumors. Using the MCF-7 breast cancer cell model we show that PMCA4 silencing results in the loss of cell polarity while a forced increase in PMCA4b expression induces cell polarization and promotes lumen formation in 2D and 3D cell cultures. We identify Arf6 as a novel regulator of PMCA4b endocytic recycling essential for PMCA4 regulated lumen formation. Silencing of the singlepmcagene inDrosophila melanogasterlarval salivary gland destroys lumen morphology suggesting a conserved role of PMCAs in lumen morphogenesis. Our findings point to a novel role of PMCA4 in controlling epithelial cell polarity, and in the maintenance of normal glandular tissue architecture.
Ewing Sarcoma Related protein 1 recognizes R-loops by binding DNA forks
Authors: Lay Michelle A. , Thompson Valery F. , Adelakun Ajibola D. , Schwartz Jacob C. 
Published: January 2024

ABSTRACTEWSR1 (Ewing Sarcoma Related protein 1) is an RNA binding protein that is ubiquitously expressed across cell lines and involved in multiple parts of RNA processing, such as transcription, splicing, and mRNA transport. EWSR1 has also been implicated in cellular mechanisms to control formation of R-loops, a three-stranded nucleic acid structure consisting of a DNA:RNA hybrid and a displaced single-stranded DNA strand. Unscheduled R-loops result in genomic and transcription stress. Loss of function of EWSR1 functions commonly found in Ewing Sarcoma correlates with high abundance of R-loops. In this study, we investigated the mechanism for EWSR1 to recognize an R-loop structure specifically. Using electrophoretic mobility shift assays (EMSA), we detected the high affinity binding of EWSR1 to substrates representing components found in R-loops. EWSR1 specificity could be isolated to the DNA fork region, which transitions between double- and single-stranded DNA. Our data suggests that the Zinc-finger domain (ZnF) with flanking arginine and glycine rich (RGG) domains provide high affinity binding, while the RNA recognition motif (RRM) with its RGG domains offer improved specificity. This model offers a rational for EWSR1 specificity to encompass a wide range in contexts due to the DNA forks always found with R-loops.
Which came first, work-personal conflict or work environment? Extending the generational debate on cohort differences in turnover intention
Authors: Pittman Michael , Jung Sangwon (Sean) , Gordon Susan Elizabeth 
Published: January 2024

Purpose
This study aims to examine the sequential effects of work–personal conflict (WPC) and work environment (WE) on turnover intention (TI) with a focus on generational differences in the restaurant context.

Design/methodology/approach
To test the two-model approach, this study uses a moderated mediation analysis based on developed scenarios for survey questionnaires completion by participants.

Findings
The results found that WE and personal–work conflict each have a unique sequential effect on TI. However, younger generations perceived external conflicts to affect their personal lives more than older generations. For older generations, external conflict affecting personal life had caused higher intentions to quit their jobs.

Originality/value
The study provides the unique contribution of studying the sequential effects of WPC and WE on TI. Furthermore, this study helps to fill the gap of generational research by testing generational perceptions of these relationships.
Narrow gap welding seam deflection correction study based on passive vision
Authors: Zhang Wang , Fan Lizhe , Guo Yanbin , Liu Weihua , Ding Chao 
Published: January 2024

Purpose
The purpose of this study is to establish a method for accurately extracting torch and seam features. This will improve the quality of narrow gap welding. An adaptive deflection correction system based on passive light vision sensors was designed using the Halcon software from MVtec Germany as a platform.

Design/methodology/approach
This paper proposes an adaptive correction system for welding guns and seams divided into image calibration and feature extraction. In the image calibration method, the field of view distortion because of the position of the camera is resolved using image calibration techniques. In the feature extraction method, clear features of the weld gun and weld seam are accurately extracted after processing using algorithms such as impact filtering, subpixel (XLD), Gaussian Laplacian and sense region for the weld gun and weld seam. The gun and weld seam centers are accurately fitted using least squares. After calculating the deviation values, the error values are monitored, and error correction is achieved by programmable logic controller (PLC) control. Finally, experimental verification and analysis of the tracking errors are carried out.

Findings
The results show that the system achieves great results in dealing with camera aberrations. Weld gun features can be effectively and accurately identified. The difference between a scratch and a weld is effectively distinguished. The system accurately detects the center features of the torch and weld and controls the correction error to within 0.3mm.

Originality/value
An adaptive correction system based on a passive light vision sensor is designed which corrects the field-of-view distortion caused by the camera’s position deviation. Differences in features between scratches and welds are distinguished, and image features are effectively extracted. The final system weld error is controlled to 0.3 mm.
Practices and strategies for global sourcing and supply chain management: a Pareto analysis and MOORA a mixed method approach
Authors: Raj Rohit , Kumar Vimal , Mittal Ankesh , Verma Priyanka , Lai Kuei-Kuei , Singh Arpit 
Published: January 2024

Purpose
This study aims to identify and prioritize the key practices and strategies for effective global sourcing and supply chain management (SCM).

Design/methodology/approach
The study uses a combination of Pareto analysis and multi-objective optimization based on ratio analysis research methodology to analyze and establish the relationships among the identified key practices and strategies. Pareto analysis enables organization to prioritize organizational efforts and resources by focusing on the most critical factors.

Findings
The study shows that the “eco-friendly sourcing strategy”, “lean manufacturing” and “tool cost analysis” are the top critical practices and strategy variables for global sourcing and SCM, whereas the “risk management”, “procurement strategy” and “leverage digital solutions” are the critical practices and strategy variables.

Research limitations/implications
The findings of this research can also assist organizations in making informed decisions to optimize their global sourcing and supply chain operations.

Originality/value
By using these methods, this research paper gives valuable insights into the critical practices and strategies that can enhance efficiency, mitigate risks and drive success in global sourcing and SCM. The subjects and elements this study identified will serve as a framework and suggestions for further theoretical investigation and real-world implementations.
Blockchain’s double-edged sword: thematic review of illegal activities using blockchain
Authors: Sangal Shreya , Duggal Gaurav , Nigam Achint 
Published: January 2024

Purpose
The purpose of this research paper is to review and synthesize the role of blockchain technology (BCT) in various types of illegal activities, including but not limited to fraud, money laundering, ransomware attacks, firearms, drug tracking, cyberattacks, identity theft and scams.

Design/methodology/approach
The authors conducted a review of studies related to illegal activities using blockchain from 2015 to 2023. Next, a thematic review of the literature was performed to see how these illegal activities were conducted using BCT.

Findings
Through this study, the authors identify the relevant themes that highlight the major illegal activities performed using BCT, its possible steps for prevention and the opportunities for future developments. Finally, the authors provide suggestions for future research using the theory, context and method framework.

Originality/value
No other research has synthesized the illegal activities using BCT through a thematic approach to the best of the authors’ knowledge. Hence, this study will act as a starting point for future research for academic and technical practitioners in this area.
Examining the impact of working conditions, lifestyle choices, and demographic factors on mental health of industrial workers
Authors: Daghagh Yazd Sahar , Karaman Mehmet Akif , Fathi Salma , Alsarraf Areej , Alajmi Shaikhah , Rutabian Sahab , Aladwani Manya 
Published: January 2024

Purpose
This study aims to explore how factors such as working conditions, lifestyle choices and demographic characteristics may affect the mental health of industrial workers in Kuwait.

Design/methodology/approach
Within the scope of the study’s objective, the authors reached 400 industrial workers (228 male, 172 female) working in oil and gas organizations in Kuwait. An ordered logistic regression model was conducted to examine the effect of relevant independent variables on workers’ mental health.

Findings
Result indicated that employees with a lower education level (p-value = 0.015), employees who were smokers (p-value = 0.004) and employees with night shifts (p-value = 0.019), were more likely to experience mental health issues. Furthermore, result strongly highlights a higher chance of experiencing mental health problems among the workers who had longer working times (p-value = 0.000). On the other hand, having children (p-value = 0.042), visiting a mental health professional (p = 0.014) and living in a house with others (flat mate, family members, etc.) (p-value = 0.016) were statistically significant factors to improve employees’ mental health. Surprisingly, the authors’ research did not find a significant relationship between the mental health issues experienced by employees and their yearly income.

Originality/value
The paper lies in its exploration of the impact of working conditions, lifestyle choices and demographic factors on mental health of industrial workers using statistical approach. By applying ordered logistic regression, this study uncovers new insights into mental health of industrial workers.
A conceptual non-stop operation strategy for urban rail transit: a case study of São Paulo’s monorail line
Authors: Oliveira Rubens C.N. , Zhang Zhipeng 
Published: January 2024

Purpose
The purpose of this study is to address the extended travel time caused by dwelling time at stations for passengers on traditional rail transit lines. To mitigate this issue, the authors propose the “Non-stop” design, which involves trains comprised of modular vehicles that can couple and uncouple from each other during operation, thereby eliminating dwelling time at stations..

Design/methodology/approach
The main contributions of this paper are threefold: first, to introduce the concept of non-stop rail transit lines, which, to the best of the authors’ knowledge, has not been researched in the literature; second, to develop a framework for the operation schedule of such a line; and third, the author evaluate the potential of its implementation in terms of total passenger travel time.

Findings
The total travel time was reduced by 6% to 32.91%. The results show that the savings were more significant for long commutes and low train occupancy rates.

Research limitations/implications
The non-stop system can improve existing lines without the need for the construction of additional facilities, but it requires technological advances for rolling stock.

Originality/value
To eliminate dwelling time at stations, the authors present the “Non-stop” design, which is based on trains composed of locomotives that couple and uncouple from each other during operation, which to the best of the authors’ knowledge has not been researched in the literature.
Effect of sesame protein/PVA nanofibers on oil separation and rheological properties in sesame paste
Authors: Yuzer Mustafa Onur , Genccelep Huseyin 
Published: January 2024

AbstractThis study aimed to prevent or minimize the oil phase separation that occurs during storage (0‐90 days) of sesame paste at room temperature. In order to determine the changes during storage in the sesame paste samples, the zeta potential, rheological properties, and the separated oil ratio were determined. Significant (p < 0.01) effects of applied storage time and additives on the amount of oil separated in sesame pastes were determined. The oil separated in sesame paste samples varied between 3.83% and 16.29%. While the rate of separated oil increased three times in the control group over time, it showed an insignificant (p > 0.05) increase especially in the samples where 2% nanofiber was added. It was determined that the separation of the oil phase from the structure could be prevented up to 24.73% with the addition of SPI and up to 63.02% with addition of SPINL (nanofiber containing protein isolate) compared to the control.Practical applicationsDuring storage, the particles in the sesame paste tend to precipitate, causing oil separation and residue cake, thus negatively affecting the acceptability of consumers. Additives were added in order to prevent or minimize oil phase separation, which occurs in sesame paste stored at room temperature and is not desired by consumers. As additives, nanofibers containing sesame proteins produced by the electrospinning method and sesame protein isolates (SPIs) were added. Added additives acted by preventing the separation of the oil phase from the structure. It has been determined that the addition of SPI to sesame pastes does not completely prevent the problem of oil separation in sesame paste, but is effective in delaying and reducing it. Since the oil decomposition can be reduced, the amount of oil separated and therefore the part that can sink to the bottom will decrease, so sesame paste will be consumed more appropriately and will contribute to the country's economy.
Does inequality migrate? The development of income inequality across German states
Authors: Badunenko Oleg , Popova Maria 
Published: January 2024

AbstractThis study analyzes the evolution of educational and occupational patterns among migrants and natives, as well as income inequality in Germany from 1985 to 2015. We show that despite migrants catching up in education, employment, and income with their native counterparts, unfavorable societal attitudes toward them have remained virtually unchanged, which can be attributed to Bourdieu's conceptualization of cultural inheritance. We find that while income inequality has increased significantly over the 30‐year period, this trend varied considerably by the federal state and that migration did nothing to add to inequality. Since both the German economy and society rely on migrants, there is a strong need for the narratives toward migrants to be based on empirical evidence. The findings of this study hold migrant‐related policy implications not only for Germany but also for other developed nations that rely on migrants as a labor force.
An application of the reasoned action approach to clinical students' intention toward a career in geriatrics
Authors: Karikari Grace , Lohrmann David K. , Huber Lesa , Adamek Margaret , Omodior Oghenekaro 
Published: January 2024

AbstractGuided by the reasoned action approach, this study examined select individual, information, and social factors that influence intention toward pursuing a career in geriatrics among 314 clinical medical and nursing students in Ghana. A Poisson regression showed attitude toward older persons was a significant influencing factor of intention to choose a career in geriatrics for medical students (B = 0.015, SE = 0.0048, p = 0.002) but not nursing students (B = 0.009, SE = 0.0145, p = 0.512). Personal interest was, however, a significant influencing factor for both medical and nursing students (B = 0.462, SE = 0.0592, p = 0.000) and (B = 0.015, SE = 0.0048, p = 0.002), respectively. Nursing students with moderate to strong interest were 1.6 times more likely to express an intention to specialize in geriatrics, and medical students were 1.5 times more likely to express an intention to specialize in geriatrics. The results show that the most important factor influencing geriatric career intention is students' personal interest in the field. Evidence‐based interventions such as early educational and practice exposure to the field and interactions with older adults are recommended.
Managing risk and resilience in autonomous and intelligent systems: Exploring safety in the development, deployment, and use of artificial intelligence in healthcare
Authors: Macrae Carl 
Published: January 2024

AbstractAutonomous and intelligent systems (AIS) are being developed and deployed across a wide range of sectors and encompass a variety of technologies designed to engage in different forms of independent reasoning and self‐directed behavior. These technologies may bring considerable benefits to society but also pose a range of risk management challenges, particularly when deployed in safety‐critical sectors where complex interactions between human, social, and technical processes underpin safety and resilience. Healthcare is one safety‐critical sector at the forefront of efforts to develop and deploy intelligent technologies, such as through artificial intelligence (AI) systems intended to automate key aspects of healthcare tasks such as reading medical images to identify signs of pathology. This article develops a qualitative analysis of the sociotechnical sources of risk and resilience associated with the development, deployment, and use of AI in healthcare, drawing on 40 in‐depth interviews with participants involved in the development, management, and regulation of AI. Qualitative template analysis is used to examine sociotechnical sources of risk and resilience, drawing on and elaborating Macrae's (2022, Risk Analysis, 42(9), 1999–2025) SOTEC framework that integrates structural, organizational, technological, epistemic, and cultural sources of risk in AIS. This analysis explores an array of sociotechnical sources of risk associated with the development, deployment, and use of AI in healthcare and identifies an array of sociotechnical patterns of resilience that may counter those risks. In doing so, the SOTEC framework is elaborated and translated to define key sources of both risk and resilience in AIS.