bluuebunny/crossref_metadata_2025_split huggingface dataset:
ER-to-Golgi traffickingviaa dynamic intermediatecis-Golgi tubular network in Arabidopsis
Authors: Fougère Louise , Grison Magali , Laquel Patricia , Montrazi Matheus , Cordelières Fabrice , Fernández-Monreal Mónica , Poujol Christel , Uemura Tomohiro , Nakano Akihiko , Ito Yoko , Boutté Yohann 
Published: October 2023

SummaryEndoplasmic Reticulum (ER)-to-Golgi trafficking is a central process of the secretory system of eukaryotic cells that ensures proper spatiotemporal sorting of proteins and lipids1–5. However, the nature of the ER-Golgi Intermediate Compartments (ERGIC) and the molecular mechanisms mediating the transition between the ERGIC and the Golgi, as well as the universality of these processes amongst Eukaryotes, remain undiscovered. Here, we took advantage of the plant cell system in which the Golgi is highly dynamic and in close vicinity to the ER6–9. We discovered that the ERGIC is composed from at least two distinct subpopulations ofcis-Golgi. A subpopulation is a reticulated tubulo-vesicular network mostly independent from the Golgi, highly dynamic at the ER-Golgi interface and crossed by ER-induced release of luminal cargos at early stage. Another subpopulation is more stable, cisterna-like and mostly associated to the Golgi. Our results identified that the generation and dynamics of the ER-Golgi intermediate tubulo-vesicular network is regulated by the acyl-chain length of sphingolipids as well as the contacts it establishes with existing Golgi cisternae. Our study is a major twist in the understanding of the Golgi by identifying that the ERGIC in plants is a Golgi-independent highly dynamic tubular network from which arise more stable cisternae-like Golgi structures. This novel model presents a mechanism for early secretory trafficking adapted to respond to developmental and environmental stimuli, including susceptibility or resistance to diseases, autophagy or cell-reprograming.
SART1 localizes to spindle poles forming a SART1 cap and promotes spindle pole assembly
Authors: Yokoyama Hideki , Moreno-Andrés Daniel , Takizawa Kaoru , Chu Zhenzhen , Scheufen Anja , Funabashi Tsumugi , Ma Jian , Antonin Wolfram , Gruss Oliver J. , Haramoto Yoshikazu 
Published: October 2023

AbstractThe nuclear protein SART1 has been associated with pre-mRNA splicing but SART1 RNAi knockdown results also in defects in mitotic progression, centrosome biogenesis and chromosome cohesion. The mitotic roles of SART1 have not been characterized in detail and it remains unclear whether SART1 functions in mitosis directly or indirectly via pre-mRNA splicing. Here, we identify SART1 as a direct, mitosis-specific microtubule-associated protein. SART1 downregulation in human cells leads to spindle assembly defects with reduced microtubule dynamics, lack of end-on attachment, and checkpoint activation, while microtubule dynamics remain unaffected in interphase. SART1 uniquely localizes to the distal surface of mitotic centrosomes along the spindle axis, forming a previously not described structure we refer to as SART1 cap. Immunoprecipitation of SART1 consistently identifies centrosomal proteins as interaction partners. Immunostaining of these shows that SART1 downregulation does not affect centriole duplication and centrosome-accumulation of γ-tubulin but reduces the accumulation of selective pericentriolar material (PCM) proteins like Ninein. Depletion of SART1 from frog egg extracts disrupts spindle pole assembly around sperm nuclei and DNA-coated beads. Spindles formed around DNA-coated beads do not contain centrosomes but still recruits PCM proteins for spindle pole assembly. We finally show that the N-terminus of SART1 is its microtubule-binding region and essential for spindle assembly. Our data unravel a unique localization of SART1 and a novel function to recruit selective PCM proteins for spindle pole assembly in centrosomal and acentrosomal spindle assembly.
The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregation
Authors: Paim Lia Mara Gomes , Lopez-Jauregui Azriel Abraham , McAlear Thomas S. , Bechstedt Susanne 
Published: October 2023

AbstractRegulation of microtubule dynamics by microtubule-associated proteins (MAPs) is essential for mitotic spindle assembly and chromosome segregation. Altered microtubule dynamics, particularly increased microtubule growth rates, were found to be a contributing factor for the development of chromosomal instability, which potentiates tumorigenesis. The MAP XMAP215/CKAP5 is the only known microtubule growth factor, and whether other MAPs regulate microtubule growth in cells is unclear. Our recentin vitroreconstitution experiments have demonstrated that Cytoskeleton-Associated Protein 2 (CKAP2) increases microtubule nucleation and growth rates, and here we find that CKAP2 is also an essential microtubule growth factor in cells. By applying CRISPR-Cas9 knock-in and knock-out as well as microtubule plus-end tracking live cell imaging, we show that CKAP2 is a mitotic spindle protein that ensures faithful chromosome segregation by regulating microtubule growth. Live cell imaging of endogenously-labelled CKAP2 showed that it localizes to the spindle during mitosis, and rapidly shifts its localization to the chromatin upon mitotic exit before being degraded. Cells lacking CKAP2 display reduced microtubule growth rates and an increased proportion of chromosome segregation errors and aneuploidy that may be a result of an accumulation of kinetochore-microtubule mis-attachments. Microtubule growth rates and chromosome segregation fidelity can be rescued upon CKAP2 expression in knock-out cells, revealing a direct link between CKAP2 expression and microtubule dynamics. Our results unveil a role of CKAP2 in regulating microtubule growth in cells and provide a mechanistic explanation for the oncogenic potential of CKAP2 misregulation.Significance statementCell division is accomplished by the assembly of a mitotic spindle composed of microtubules that segregate the chromosomes. Cells with altered microtubule dynamics frequently mis-segregate chromosomes and develop aneuploidy, which contributes to cancer development. However, how microtubule dynamics are regulated in cells is not entirely understood. Here, using CRISPR-Cas9 genome editing and live cell imaging, we find that the microtubule-associated protein CKAP2 tightly regulates microtubule growth and ensures the proper segregation of chromosomes. Cells lacking CKAP2 develop errors in chromosome segregation and aneuploidy due to a substantial decline in microtubule growth rates. The essential role of CKAP2 in the regulation of microtubule growth provides an explanation for the oncogenic potential of CKAP2 misregulation.ClassificationBiological Sciences – Cell Biology
A time-resolved Förster resonance energy transfer assay to investigate inhibitor binding to ABCG2
Authors: Mitchell-White James I. , Briggs Deborah A. , Mistry Sarah J. , Mbiwan Hannah A , Kellam Barrie , Holliday Nicholas D. , Briddon Stephen J. , Kerr Ian D. 
Published: October 2023

AbstractThe human ATP-binding cassette (ABC) transporter, ABCG2 is responsible for multidrug resistance in some tumours. Detailed knowledge of its activity is crucial for understanding drug transport and resistance in cancer, and has implications for wider pharmacokinetics. The binding of substrates and inhibitors is a key stage in the transport cycle of ABCG2. Here, we describe a novel binding assay using a high affinity fluorescent inhibitor based on Ko143 and time-resolved Förster resonance energy transfer (TR-FRET) to measure saturation binding to ABCG2. This binding is displaced by Ko143 and other known ABCG2 ligands, and is sensitive to the addition of AMP-PNP, a non-hydrolysable ATP analogue. This assay complements the arsenal of methods for determining drug:ABCG2 interactions and has the possibility of being adaptable for other multidrug pumps.HighlightsABCG2 is a multidrug pump which moves between states having low or high affinity for substrates and inhibitorsWe introduce a time-resolved Förster resonance energy transfer assay to measure interaction of substrates and inhibitors to ABCG2We confirm that NBD dimerization is associated with a switch from a high to a low affinity site for an ABCG2 inhibitor
Fine-Tuning and Remodelling of Pectins Play a Key Role in the Maintenance of Cell Adhesion
Authors: Grandjean Cyril , Voxeur Aline , Chabout Salem , Jobert François , Gutierrez Laurent , Pelloux Jérôme , Mouille Gregory , Bouton Sophie 
Published: October 2023

ABSTRACTPlant cell adhesion is essential for development and stress response, mediated by pectin-rich middle lamella deposition between cell walls. However, the precise control mechanism of cell adhesion remains unclear. Thequa2-1andesmd1-1mutants provide a better understanding of this process and suggest a signaling pathway triggering the loss and restoration of adhesion via cell wall modifications. This study attempts to characterize the potential regulatory role of endogenous oligogalacturonides (OGs) and pectin modifications in the control of cell adhesion in Arabidopsis.From dark-grown hypocotyls, our extraction revealed seven distinct endogenous OGs with varying polymerization and modifications. Abundance variations of OGs were observed among wild type,qua2-1,esmd1-1, andqua2-1/esmd1-1mutants. The structure of homogalacturonans was analyzed by enzymatic fingerprint, in order to identify changes in esterification patterns. Expression analysis of pectin-modifying enzymes showed significant variations inPME,PMEI, andPAEgenes. Gene expressions correlate with homogalacturonans modifications and cell adhesion phenotypes.This study enhances our understanding of a feedback loop between the endogenous OGs, homogalacturonans esterification fine tuning, and pectin remodeling enzymes expression in controlling cell adhesion.
Illuminating stomatal responses to red light: establishing the role ofCi-dependent vs - independent mechanisms
Authors: Taylor Georgia , Walter Julia , Kromdijk Johannes 
Published: October 2023

AbstractThe stomatal response to red light appears to link stomatal conductance (gs) with photosynthetic rates. Initially, it was suggested that changes in intercellular CO2(Ci) provide the main cue via aCi-dependent response. However, evidence forCi-independent mechanisms suggests an additional, more direct relationship with photosynthesis. While bothCi-dependent and -independent mechanisms clearly function in stomatal red-light responses, little is known regarding their relative contribution. The present study aimed to quantify the relative magnitude ofCi-dependent and - independent mechanisms on the stomatal red-light response, to characterise their interplay and to assess the putative link between plastoquinone (PQ) redox state andCi-independent stomatal responses. Red light response curves measured at a range ofCivalues for wild-typeArabidopsis thaliana(Col-0) and the CO2hyposensitive mutant,ca1ca4, allowed deconvolution ofCi-dependent and -independent pathways. Surprisingly, we observed that both mechanisms contribute equally to stomatal red-light responses, butCi-independent stomatal opening is suppressed at highCi. The present data are also consistent with the involvement of PQ redox in coordinatingCi-independent stomatal movements. Overall, it seems that whileCi-independent mechanisms are distinct from responses toCi,interplay between these two pathways is important to facilitate effective coordination betweengsand photosynthesis.HighlightBy controlling intercellular CO2(Ci) at a range of values across red-light response curves, we demonstrate independent and interactive roles ofCi-dependent and –independent pathways in coordinating stomatal red-light responses.
The impact of unanticipated wealth effects on consumption: evidence from Spanish panel data
Authors: Cutanda Antonio , Sanchis Llopis Juan Alberto 
Published: October 2023

Purpose
The purpose of this study is to estimate the housing wealth effect on non-durable consumption using data from the Spanish Survey of Household Finances (Encuesta Financiera de las Familias, SHF) for the period 2002–2017.

Design/methodology/approach
The authors aim at identifying the effect of anticipated and unanticipated housing wealth changes on consumption with the sample of homeowners, following Paiella and Pistaferri (2017).

Findings
Results of this study lead us to conclude that there exists a strong housing wealth effect on consumption for the Spanish households.

Originality/value
The authors provide evidence against the permanent income model. They also analyse how the results change with income expectations, age and the household indebtedness rate. Finally, they detect a strong excess sensitivity to income.
Characteristics of business, pleasure, and VFR segments who travel during risky times
Authors: Tasci Asli D.A. , Milman Ady 
Published: October 2023

Purpose
This study aims to explore what may have contributed to risk-taking travel behavior during the COVID-19 pandemic and what may be the drivers of satisfaction and intention to travel again. The study compares travelers based on their travel purposes (business, pleasure and visiting friends and relatives [VFR]) and explore the influence of subjective well-being received from their travel activities.

Design/methodology/approach
Data from a structured survey of 323 US residents who traveled during the COVID-19 pandemic were collected on MTurk. Respondents were asked travel behavior questions related to their favorite trips and to rate several multi-item scales measuring the benefits expected and received from their trip, the trip’s contribution to their positive mental and subjective psychological well-being, as well as their satisfaction with the trip and intention to travel in the future. In addition, personality measures focused on risk-taking, thrill-seeking and self-confidence, as well as additional risk-related concepts of optimism bias, probability neglect and proximity to self.

Findings
Data analysis revealed some differences among respondents who traveled for business, pleasure and VFR purposes. Business travelers were more risk takers and thrill seekers; pleasure travelers achieved more well-being benefits from their favorite trips during the pandemic; however, VFR travelers’ satisfaction and intention to go on similar trips were explained more by the benefits they received from their favorite trips.

Originality/value
Although several studies addressed consumers’ travel motivation during the COVID-19 pandemic, there is a lack of empirical research comparing the characteristics of travelers based on their travel purposes, as well as their sociodemographics, personality traits and the expected and perceived well-being benefits from traveling.
Explainable boosted combining global and local feature multivariate regression model for deformation prediction during braced deep excavations
Authors: Zhang Wenchao , Shi Peixin , Wang Zhansheng , Zhao Huajing , Zhou Xiaoqi , Jia Pengjiao 
Published: October 2023

PurposeAn accurate prediction of the deformation of retaining structures is critical for ensuring the stability and safety of braced deep excavations, while the high nonlinear and complex nature of the deformation makes the prediction challenging. This paper proposes an explainable boosted combining global and local feature multivariate regression (EB-GLFMR) model with high accuracy, robustness and interpretability to predict the deformation of retaining structures during braced deep excavations.Design/methodology/approachDuring the model development, the time series of deformation data is decomposed using a locally weighted scatterplot smoothing technique into trend and residual terms. The trend terms are analyzed through multiple adaptive spline regressions. The residual terms are reconstructed in phase space to extract both global and local features, which are then fed into a gradient-boosting model for prediction.FindingsThe proposed model outperforms other established approaches in terms of accuracy and robustness, as demonstrated through analyzing two cases of braced deep excavations.Research limitations/implicationsThe model is designed for the prediction of the deformation of deep excavations with stepped, chaotic and fluctuating features. Further research needs to be conducted to expand the model applicability to other time series deformation data.Practical implicationsThe model provides an efficient, robust and transparent approach to predict deformation during braced deep excavations. It serves as an effective decision support tool for engineers to ensure the stability and safety of deep excavations.Originality/valueThe model captures the global and local features of time series deformation of retaining structures and provides explicit expressions and feature importance for deformation trends and residuals, making it an efficient and transparent approach for deformation prediction.
Literacy instructional coaching practices in writing and writing instruction: an exploration of K–6 teachers' perspectives
Authors: Abbott Jadelyn , Wright Katherine Landau , Carter Hannah 
Published: October 2023

PurposeThe purpose of this study was to identify if and how K–6 teachers perceive that their literacy instructional coaches influence their writing teaching.Design/methodology/approachThe authors employed a parallel convergent mixed-methods design with survey data. The authors used thematic analysis to identify patterns within short-answer responses.FindingsK–6 teachers receive little literacy coaching specific to writing. However, when they do receive coaching, they believe it benefits their writing instruction. Sustained coaching through the coaching cycle, frequent collaborations, and support with writing instructional resources and strategies were reported as the most influential writing coaching practices.Research limitations/implicationsSample size was a limitation to this study. Of the 66 participants, 41 (62%) completed the entire survey.Practical implicationsThis research provides coaches with valuable insights about coaching practices that teachers find to be the most effective in influencing their writing instruction. The increase in teachers' competence in writing instruction due to coaching provides evidence to administrators and stakeholders that coaching in writing is an area in need of attention.Originality/valueThis study adds to research specific to the coaching of writing within the K–6 context, which currently is sparse.
UnDoing: a research-through-curation project that investigates the reuse of the built environment
Authors: Stone Sally Helen , Sanderson Laura 
Published: October 2023

Purpose This paper considers the exhibition: UnDoing. This research-through-curation project examined interactions within existing spaces and situations. This established links between the selected exhibits, the gallery, the city and with the continuum of the previous exhibition.Design/methodology/approach Carefully selected architects, designers and artists were invited to contribute—those who pursued a contextual approach; whose practice explored the way buildings, places and artefacts are reused, reinterpreted and remembered.FindingsThrough the act of curation, this research uncovered a series of different approaches to constructed sites and existing buildings, from layered juxtaposition, the refusal to undo, to interventions of new elements within architectural works.Research limitations/implications Curation offered the opportunity to consider works of architecture and of art through the same lens, for direct comparisons to be made and the influence of one upon the other to be comprehended.Practical implications The examination processes the architect employs is similar to that of the artist; the development of an understanding of place, and from this synthesis, creative interpretation. However, despite the similarities in the starting position, the elucidation developed by the artist can be vastly different to that of the architect.Social implications The juxtaposition and new classifications created by the exhibition encouraged visitors to look at art, architecture and the city in a different way; to grasp the direct link between the different subjects; and the possibilities created.Originality/value The two driving factors for UnDoing were places of previous occupation and the city of Manchester. The qualities of surrounding constructed environment combined were combined with attitudes towards existing structures and places.
Palate vs plate: segmenting restaurant consumers through food quality and portion size trade-offs
Authors: Ge Li , Tang Chun-Hung (Hugo) , Behnke Carl , Ghiselli Richard 
Published: October 2023

PurposeThis study (1) assessed restaurant consumers' perceived importance of better food quality versus larger portion sizes, (2) classified restaurant consumers into different segments based on their perceived importance of seven food quality attributes (taste, texture, aroma, appearance and the use of natural, local and organic ingredients) relative to portion size and (3) compared the identified market segments.Design/methodology/approachAn online survey assessed 613 USA adult participants' perceived importance of seven food quality attributes relative to portion size. A K-means cluster analysis classified participants into different segments based on their perceptions.FindingsFive restaurant consumer segments were identified: sensory-oriented consumers, taste-oriented organic food consumers, local and natural food consumers, quantity-oriented consumers and quality-oriented consumers. In general, quality-oriented consumers were the least likely to visit fast-food restaurants, had the highest average per-meal spending and were the least likely to eat out alone. Local and natural food consumers and taste-oriented organic food consumers had higher total restaurant spending than other groups. Quantity-oriented and sensory-oriented consumers visited fast-food restaurants more often and had lower total restaurant spending and per-meal spending than other groups. Age, sex and income were significantly associated with the segmentation outcomes.Practical implicationsFindings suggest promising opportunities for restaurants to enhance consumer perceptions of value by prioritizing the factors that hold the greatest significance to their target customers.Originality/valueThis study is the first attempt to segment restaurant consumers based on their perceived importance of food quality attributes relative to portion size, effectively identifying five distinct consumer segments.