bluuebunny/crossref_metadata_2025_split huggingface dataset:
A arte no estado de encontros com o processo criativo de Sérgio Moura
Authors: Bressan Regiane , Sirino Salete Paulina Machado 
Published: December 2023

Neste artigo, objetiva-se apresentar a arte no estado de encontros com o processo criativo de Sergio Moura, e a mediação entre os artistas e a sociedade para que a potência criativa flua de forma independente. Portanto, consiste na reflexão do seu projeto Artshow, e sobre a urgência de cultivar a sensibilidade criadora com a estranha realidade nacional, e na abordagem de cada um no todo, no coletivo. Dessa forma, o trabalho de Sergio Moura contempla o coletivo carregado de teor crítico devido à persistência em realizar projetos relacionais e vivenciais, frequentemente transitórios, ao longo dos anos no campo artístico em geral, ocorridos durante o período ditatorial militar. Nesse sentido, ao estabelecer um espaço comum para compartilhar desse lugar enquanto sociedade e devolver para o receptor o olhar em volta, seja encontrando semelhanças ou desacordos com a teoria do filósofo Jacques Rancière, pretende-se propor uma leitura da estética relacional na abordagem dos materiais analisados a partir das principais referências: Luise Boeno Malmaceda, Maria José Justino, Artur Freitas e Jacques Rancière.
Palavras-Chave: Arte; Coletivo; Crítico; Ruptura; Liberdade Criativa.
Assessing body condition scores, weight gain dynamics, and fecal egg counts in feedlot and non-feedlot cattle within high throughput abattoirs of the Eastern Cape Province
Authors: Mpofu Beautiful Isabel , Slayi Mhlangabezi , Mutero Gabriel , Mlahlwa Sisipho , Jaja Ishmael Festus 
Published: December 2023

IntroductionFeedlots have emerged as an ideal mitigation option to pursue sustainable and efficient livestock production. This paper aims to elucidate how the establishment and widespread adoption of feedlots have provided solutions to complex problems of food security, animal welfare, and environmental sustainability.MethodsAn observational cross-sectional study compared fecal egg count per gram (EPG), weight gain, and body condition score (BCS) in feedlot and non-feedlot cattle at high throughput abattoirs. Cattle (n = 120) of different age and sex groups, farms, and breeds were selected from two commercial abattoirs (EA1 and EA2) in the Eastern Cape Province.ResultsAt EA1, non-feedlot cattle exhibited higher EPG values (323.3±28.9) than feedlot cattle (73.3±13.3), indicating a potentially greater susceptibility to internal parasitic infections. The similar weight gains between feedlot (298.1±4.7) and non-feedlot cattle (287.16±7.79) were attributed to the sufficient natural pasture in the communally raised cattle. However, body condition scores were significantly (P&lt; 0.05) better in feedlot than in non-feedlot cattle. Nonetheless, the feedlot farms of origin had a significant effect (P&lt; 0.05) on the EPG and body condition score values in EA1, with no significant effect in EA2.DiscussionMoreover, the negative correlation between EPG and body condition scores highlights that as parasite load increases, there might be a subtle tendency for body condition to decrease. These results underscore the importance of feedlots as an effective management strategy to improve animal health and productivity. Further investigations into the factors driving the differences in non-feedlot cattle are needed for informed decision-making in livestock management and abattoir operations.
Toolkit for cellular studies of mammalian mitochondrial inorganic polyphosphate
Authors: Hambardikar Vedangi , Akosah Yaw A. , Scoma Ernest R. , Guitart-Mampel Mariona , Urquiza Pedro , Da Costa Renata T. , Perez Matheus M. , Riggs Lindsey M. , Patel Rajesh , Solesio Maria E. 
Published: December 2023

Introduction: Inorganic polyphosphate (polyP) is an ancient polymer which is extremely well-conserved throughout evolution, and found in every studied organism. PolyP is composed of orthophosphates linked together by high-energy bonds, similar to those found in ATP. The metabolism and the functions of polyP in prokaryotes and simple eukaryotes are well understood. However, little is known about its physiological roles in mammalian cells, mostly due to its unknown metabolism and lack of systematic methods and effective models for the study of polyP in these organisms.Methods: Here, we present a comprehensive set of genetically modified cellular models to study mammalian polyP. Specifically, we focus our studies on mitochondrial polyP, as previous studies have shown the potent regulatory role of mammalian polyP in the organelle, including bioenergetics, via mechanisms that are not yet fully understood.Results: Using SH-SY5Y cells, our results show that the enzymatic depletion of mitochondrial polyP affects the expression of genes involved in the maintenance of mitochondrial physiology, as well as the structure of the organelle. Furthermore, this depletion has deleterious effects on mitochondrial respiration, an effect that is dependent on the length of polyP. Our results also show that the depletion of mammalian polyP in other subcellular locations induces significant changes in gene expression and bioenergetics; as well as that SH-SY5Y cells are not viable when the amount and/or the length of polyP are increased in mitochondria.Discussion: Our findings expand on the crucial role of polyP in mammalian mitochondrial physiology and place our cell lines as a valid model to increase our knowledge of both mammalian polyP and mitochondrial physiology.
Evidence-based decision-making in speech-language pathology via video-based telepractice—A qualitative video interaction analysis
Authors: Barthel Maria , Wittmar Silke , Borgetto Bernhard , Leinweber Juliane 
Published: December 2023

IntroductionIn speech-language pathology, there is a constant need to make evidence-based decisions based on the patient's needs and goals, speech-language pathologist's clinical expertise, and external evidence. During the COVID-19 pandemic, it was possible for the first time in Germany to implement video-based telepractice in the outpatient care of speech-language pathology. This study aimed to find out how evidence-based decisions are made in video-based telepractice and what forms of evidence are used.MethodsSpeech-language pathologists who were working in outpatient services recorded their video-based telepractices for the research project. Five recorded video-based telepractices were transcribed using a simple transcription system and video interaction analysis was used to analyze the video recordings based on grounded theory methodology.ResultsInteractions between patients and speech-language pathologists are characterized by evidence-based decisions that can be observed. Speech-language pathologists make decisions based on their clinical expertise, patient-related experiences, and patient self-assessments. There is little evidence of negotiation between patients and speech-language pathologists to make joint decisions. Results showed that speech-language pathologists do not explicitly name external evidence to justify their decisions to patients. Shared decision-making is encouraged by a participative interaction between patients and speech-language pathologists. However, there was a predominant paternalistic interaction in which the speech-language pathologists made decisions on their own. To represent the decision-based interactions between patients and speech-language pathologists in a video-based therapy session, a process model was developed.DiscussionEvidence-based and shared decision-making are important in speech-language pathology to provide patient-centered treatments. The exchange of information between the patient and the speech-language pathologist is important in order to make joint decisions based on these different levels of knowledge. In this way, the patient becomes an active participant in the digital treatment, in the video-based telepractice. As a result, the quality of care can be improved. Further research should reconstruct the implicit (possible) use of external evidence.
The paradoxes of inclusion: cognitive and socio-emotional developmental trajectories of deaf and blind primary education students in mainstream and special schools
Authors: Rosas Ricardo , Espinoza Victoria , Martinez Camila , Santa-Cruz Catalina 
Published: December 2023

IntroductionStudents with special educational needs (SEN) have historically found participating in the regular education system challenging. Since the 1990s, inclusion has been considered the optimal strategy for their development. However, the effects of inclusive education on deaf and blind students are still little studied.MethodsIn the present article, we report the results of a longitudinal study on the cognitive and socioemotional developmental trajectories of 23 deaf and 29 blind primary education students attending mainstream (8 deaf and 10 blind) and special schools (15 deaf and 19 blind). The study was conducted in Santiago de Chile between 2018 and 2019.ResultsOur descriptive results suggest that deaf students attending special schools perform better on most of the variables studied. For blind students, those attending traditional schools generally perform better than those attending special schools. However, in the case of socio-emotional variables, blind students attending special schools tend to show fewer problems. However, almost all of the indicated differences are not statistically significantly different.DiscussionWe then discuss the need to consider the characteristics of each group of students with SEN when defining an adequate educational system for their optimal development.
Performance of invasive, non-invasive, and pharmacological measures by emergency physicians in the German emergency medical services. A retrospective cross-sectional study from the rescue association Südwestsachsen
Authors: Koch Sebastian , Suffeda Michelle 
Published: December 2023

BackgroundEvery citizen in Germany is entitled to qualified emergency medical assistance. Due to annually increasing deployment figures and personnel shortages, it is no longer possible to guarantee 24/7 coverage of all emergency physician locations throughout Germany. Relief can be achieved through changes in the supply structures. Professional field analyses are the basis for the further development of training and care structures.MethodologyAll emergency physician deployments of a location in the rescue association Südwestsachsen that took place between 01.10.2022 and 31.01.2023 were retrospectively evaluated. For this purpose, the analogously created deployment protocols were examined with regard to demographic data as well as the invasive, non-invasive and pharmacological measures performed by emergency physicians.ResultsIn N = 684 emergency missions, n = 471 (100%) invasive or non-invasive measures were performed by an emergency physician. At n = 383 (81.3%), the most frequently performed invasive measure was intravenous (i.v.) access. There were n = 710 (100%) pharmacological measures performed by an emergency physician. The most frequently performed pharmacologic measure was administration of a full electrolyte solution, with n = 223 (31.4%)DiscussionEmergency physician interventions do not necessarily involve invasive, non-invasive, or pharmacologic measures. The emergency paramedic learns a variety of invasive, non-invasive, and pharmacologic measures in his or her training and could contribute to the reduction of emergency physician interventions. An adaptation of prehospital care structures based on evidence-based data and an update of the catalog of indications for emergency physician interventions would be elementary for this goal.
Insulin-like growth factor binding protein-1 and insulin in polycystic ovary syndrome: a systematic review and meta-analysis
Authors: Jin Yuxin , Sun Fei , Yang Aili , Yu Xinwen , Li Yi , Liang Shengru , Jing Xiaorui , Wang Kai , Zhang Lan , Xiao Sa , Zhang WenCheng , Wang Xiaoguang , Zhao Guohong , Gao Bin 
Published: December 2023

BackgroundInsulin-like growth factor binding protein-1 (IGFBP-1) is considered a decline in polycystic ovary syndrome (PCOS), but it remains controversial that whether such reduction is attributed to obesity.AimsThis systematic review aims to explore whether IGFBP-1 is reduced in PCOS, and whether such reduction is associated with obesity.ResultsOur pooled study included 12 studies with a total of 450 participants. IGFBP-1 levels in PCOS were significantly lower than that in non-PCOS (SMD (95%CI)=-0.49(-0.89, -0.09), P=0.02). No significant difference in IGFBP-1 levels between patients with or without PCOS classified by BMI. Whilst, stratification by PCOS status revealed a significant decrease in IGFBP-1 in overweight (SMD (95%CI)=-0.92(-1.46, -0.37), P=0.001). When comparing fasting insulin in the same way, PCOS patients had significantly elevated fasting insulin level but not statistically declined IGFBP-1 after classified by BMI.ConclusionThis meta-analysis provides evidence that the decrease of IGFBP-1 in PCOS was more strongly influenced by comorbid obesity than by PCOS itself. Additionally, contrast to previous findings that insulin significantly suppresses IGFBP-1, our results suggested that the suppression of PCOS-related hyperinsulinemia on IGFBP-1 seemed diminished. Overall, our work may provide a novel perspective on the mechanism between insulin and IGFBP-1 underlying PCOS development.
Response of spring vegetation phenology to soil freeze–thaw state in the Northern Hemisphere from 2016 to 2022
Authors: Yang Ting , Cong Nan 
Published: December 2023

IntroductionThe research on spring vegetation phenology is crucial to the investigation of terrestrial ecosystems and climate change. Changes in the soil freeze–thaw (F/T) lead to variations in soil moisture, directly impacting vegetation activity. The start of the season (SOS) is the initial and important phenophase for vegetation activity, and thus, this highlights the need to understand the response of spring vegetation phenology to soil F/T state.MethodsThis study first comprehensively investigates the consistency of the SOS and three soil F/T state indexes, i.e., the start day of the F/T state (SFT), the end day of the F/T state (EFT), and the length of days of the F/T state (LFT) via satellite data source.ResultsResults reveal that: (1) All 3 F/T state indexes impact SOS values, and the EFT outperforms others. The correlation coefficients between EFT and SOS gain around 3.07%. (2) A temporal overlap between SOS and EFT occurs in May, suggesting that parts of the plants begin active growth before average temperatures reach above 0°. (3) Small differences of SOS and EFT exist between savannas, and croplands, with an average difference of less than 10 days; in contrast, the largest differences occur in broadleaf evergreen forests. The results can fill the knowledge gap on the response of spring vegetation phenology to soil F/T state, and help to investigate the reasons for the nonlinear dynamics of SOS under global warming.
Exploring the immune microenvironment of osteosarcoma through T cell exhaustion-associated gene expression: a study on prognosis prediction
Authors: Zhu Junchao , Yuan Jinghong , Arya Shahrzad , Du Zhi , Liu Xijuan , Jia Jingyu 
Published: December 2023

BackgroundOsteosarcoma is a highly aggressive type of bone cancer with a poor prognosis. In the tumor immune microenvironment, T-cell exhaustion can occur due to various factors, leading to reduced tumor-killing ability. The purpose of this study was to construct a prognostic model based on T-cell exhaustion-associated genes in osteosarcoma.MethodsPatient data for osteosarcoma were retrieved from the TARGET and GEO databases. Consensus clustering was employed to identify two novel molecular subgroups. The dissimilarities in the tumor immune microenvironment between these subgroups were evaluated using the “xCell” algorithm. GO and KEGG analyses were conducted to elucidate the underlying mechanisms of gene expression. Predictive risk models were constructed using the least absolute shrinkage and selection operator algorithm and Cox regression analysis. To validate the prognostic significance of the risk gene expression model at the protein level, immunohistochemistry assays were performed on osteosarcoma patient samples. Subsequently, functional analysis of the key risk gene was carried out through in vitro experimentation.ResultsFour gene expression signatures (PLEKHO2, GBP2, MPP1, and VSIG4) linked to osteosarcoma prognosis were identified within the TARGET-osteosarcoma cohort, categorizing patients into two subgroups. The resulting prognostic model showed strong predictive capability, with area under the receiver operating characteristic curve (AUC) values of 0.728/0.740, 0.781/0.658, and 0.788/0.642 for 1, 3, and 5-year survival in both training and validation datasets. Notably, patients in the low-risk group had significantly higher stromal, immune, and ESTIMATE scores compared to high-risk counterparts. Additionally, a nomogram was developed, exhibiting high accuracy in predicting the survival outcome of osteosarcoma patients. Immunohistochemistry, Kaplan-Meier, and time-dependent AUC analyses consistently supported the prognostic value of the risk model within our osteosarcoma patient cohort. In vitro experiments provided additional validation by demonstrating that the downregulation of GBP2 promoted the proliferation, migration, and invasion of osteosarcoma cells while inhibiting apoptosis.ConclusionThe current study established a prognostic signature associated with TEX-related genes and elucidated the impact of the pivotal gene GBP2 on osteosarcoma cells via in vitro experiments. Consequently, it introduces a fresh outlook for clinical prognosis prediction and sets the groundwork for targeted therapy investigations in osteosarcoma.
IFN-γ-mediated control of SARS-CoV-2 infection through nitric oxide
Authors: Silva Bruno J. de Andrade , Krogstad Paul A. , Teles Rosane M. B. , Andrade Priscila R. , Rajfer Jacob , Ferrini Monica G. , Yang Otto O. , Bloom Barry R. , Modlin Robert L. 
Published: December 2023

IntroductionThe COVID-19 pandemic has highlighted the need to identify mechanisms of antiviral host defense against SARS-CoV-2. One such mediator is interferon-g (IFN-γ), which, when administered to infected patients, is reported to result in viral clearance and resolution of pulmonary symptoms. IFN-γ treatment of a human lung epithelial cell line triggered an antiviral activity against SARS-CoV-2, yet the mechanism for this antiviral response was not identified.MethodsGiven that IFN-γ has been shown to trigger antiviral activity via the generation of nitric oxide (NO), we investigated whether IFN-γ induction of antiviral activity against SARS-CoV-2 infection is dependent upon the generation of NO in human pulmonary epithelial cells. We treated the simian epithelial cell line Vero E6 and human pulmonary epithelial cell lines, including A549-ACE2, and Calu-3, with IFN-γ and observed the resulting induction of NO and its effects on SARS-CoV-2 replication. Pharmacological inhibition of inducible nitric oxide synthase (iNOS) was employed to assess the dependency on NO production. Additionally, the study examined the effect of interleukin-1b (IL-1β) on the IFN-g-induced NO production and its antiviral efficacy.ResultsTreatment of Vero E6 cells with IFN-γ resulted in a dose-responsive induction of NO and an inhibitory effect on SARS-CoV-2 replication. This antiviral activity was blocked by pharmacologic inhibition of iNOS. IFN-γ also triggered a NO-mediated antiviral activity in SARS-CoV-2 infected human lung epithelial cell lines A549-ACE2 and Calu-3. IL-1β enhanced IFN-γ induction of NO, but it had little effect on antiviral activity.DiscussionGiven that IFN-g has been shown to be produced by CD8+ T cells in the early response to SARS-CoV-2, our findings in human lung epithelial cell lines, of an IFN-γ-triggered, NO-dependent, links the adaptive immune response to an innate antiviral pathway in host defense against SARS-CoV-2. These results underscore the importance of IFN-γ and NO in the antiviral response and provide insights into potential therapeutic strategies for COVID-19.
Description of Mycobacterium pinniadriaticum sp. nov., isolated from a noble pen shell (Pinna nobilis) population in Croatia
Authors: Špičić Silvio , Duvnjak Sanja , Papić Bojan , Reil Irena , Zrnčić Snježana , Mihaljević Željko , Naletilić Šimun , Zupičić Ivana Giovanna , Kompes Gordan , Habrun Boris , Mareković Ivana , Zdelar-Tuk Maja 
Published: December 2023

IntroductionShortly before the mass mortality event of the noble pen shell (Pinna nobilis) population in the south-eastern Adriatic coast, two rapidly growing Mycobacterium strains CVI_P3T (DSM 114013 T, ATCC TSD-295 T) and CVI_P4 were obtained from the organs of individual mollusks during the regular health status monitoring.MethodsThe strains were identified as members of the genus Mycobacterium using basic phenotypic characteristics, genus-specific PCR assays targeting the hsp65 and 16S rRNA genes and the commercial hybridization kit GenoType Mycobacterium CM (Hain Lifescience, Germany). MALDI-TOF mass spectrometry did not provide reliable identification using the Bruker Biotyper Database.Results and discussionGenome-wide phylogeny and average nucleotide identity (ANI) values confirmed that the studied strains are clearly differentiated from their closest phylogenetic relative Mycobacterium aromaticivorans and other validly published Mycobacterium species (ANI ≤ 85.0%). The type strain CVI_P3T was further characterized by a polyphasic approach using both phenotypic and genotypic methods. Based on the phenotypic, chemotaxonomic and phylogenetic results, we conclude that strains CVI_P3T and CVI_P4 represent a novel species, for which the name Mycobacterium pinniadriaticum sp. nov. is proposed.
Mannan oligosaccharides improve the fur quality of raccoon dogs by regulating the gut microbiota
Authors: Yuan Chongshan , Ren Lili , Sun Rui , Yun Xianghong , Zang Xiao , Zhang Aiwu , Wu Min 
Published: December 2023

IntroductionAdding antibiotics to animal basal diets can improve growth and production performance. However, the use of antibiotics poses a potential threat to public health safety.MethodsThe study was conducted to investigate the effects of different levels of mannan oligosaccharides (MOS) on the fur quality, nutrient apparent digestibility, serum immunity, antioxidant status, intestinal morphology, and gut microbiota of fur-growing raccoon dogs. Divide 24 male raccoon dogs (120 ± 5 d) of similar weight (5.01 ± 0.52 kg) into 4 groups randomly. Add 0, 0.05, 0.1, and 0.2% MOS to the basal diets of groups C, L, M, and H, respectively.ResultsCompared to the C group, the addition of 0.05% and 0.1% MOS in the diet increased the apparent digestibility of crude protein (CP), Underfur length (UL), Guard hair length (GL), immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) levels in the serum (p &lt; 0.05); Under the dosage of 0.05 % MOS, the activities of Superoxide Dismutase (SOD) and catalase (CAT) increased (p &lt; 0.05). Compared to the C group, adding 0.05% MOS significantly increased the VH/CD of the duodenum and ileum, while also increasing the VH and CD of the jejunum (p &lt; 0.05). Through Spearman correlation analysis of the gut microbiota, it was found that MOS can improve fur quality by reducing the abundance of Dorea while improving the immune response of raccoon dogs by reducing the abundance of Blautia and Gemmiger.DiscussionIn conclusion, MOS can improve the fur quality, serum immunity, antioxidant capacity, and gut microbiota of raccoon dogs. Therefore, MOS has the potential to replace antibiotics.