bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomes ofAegilops umbellulataprovide new insights into unique structural variations and genetic diversity in the U-genome for wheat improvement
Authors: Singh Jatinder , Gudi Santosh , Maughan Peter J. , Liu Zhaohui , Kolmer James , Wang Meinan , Chen Xianming , Rouse Matthew , Lasserre-Zuber Pauline , Rimbert Helene , Sehgal Sunish , Fiedler Jason , Choulet Frédéric , Acevedo Maricelis , Gupta Rajeev , Gill Upinder 
Published: January 2024

AbstractAegilopsspp. serve as an important reservoir for novel sources of resistance or tolerance to biotic and abiotic stresses. To harness this reservoir, we have generated a high-quality chromosome-level genome assembly of anAe.umbellulataaccession PI 554389 using a combination of PacBio HiFi, Oxford nanopore, and chromosome conformation capture (Hi-C) sequencing technologies and resequenced 20Ae. umbellulatagenomes using Illumina sequencing. We assembled a 4.20 Gb genome spanned over seven chromosomes, rich in repetitive elements (∼84%), achieving a QV of 59.54 with 98.14% completeness. The phylogenetic analysis places the U-genome with D-lineage, but major and distinct rearrangements were revealed in the U-genome. Unique transposon landscape of diploid U-genome and complex chromosomal rearrangements, most prominently in 4U and 6U chromosomes uncovered a distinct evolutionary trajectory ofAe. umbellulata. Additionally, the resequencing of geographically and morphologically diverseAe. umbellulataaccessions revealed three distinctive evolutionary sub-populations. Resequencing also identified six new haplotypes forLr9, the first leaf rust resistance gene introgressed and cloned fromAe. umbellulata.These genomics resources along with high levels of resistance in the resequenced accessions against five devastating wheat diseases affirmed the genetic potential ofAe. umbellulatafor wheat improvement.
Development of a simple and highly sensitive method for the concentration and detection of African swine fever virus in oral fluids and its feasibility assessment on raised pigs in Northern Vietnam
Authors: Mai Thi Ngan , Tran Thi Huong Giang , Dong Van Hieu , Le Van Phan , Huynh Thi My Le , Bui Tran Anh Dao , Uemura Ryoko , Yamazaki Yasuko , Nguyen Thi Lan , Yamazaki Wataru 
Published: January 2024

AbstractBackgroundEarly detection and early slaughter through quarantine are essential to prevent the spread of the African swine fever virus (ASFV). Highly accurate testing is effective for early detection, but it is still difficult to establish a system, especially in the Global South. Pooled oral fluids tests have been used for simple pathogen monitoring, but compared to blood tests, the virus concentration in oral fluids is low, resulting in false negative and missing true positive cases. We collected oral fluids from raised pigs in northern Vietnam and attempted a highly sensitive ASFV survey using a newly developed virus concentration and detection method.ResultsIn a spike test, the developed method showed up to 100 times greater sensitivity than a reference method. To compare and evaluate the performance of the developed method, a total of 68 pooled oral fluid samples were collected, 63 from northern Vietnam and 5 from southern Japan. Using real-time PCR, 9/68 (13.2%) were positive by the reference method, and 23/68 (33.8%) by the developed method. Using real-time LAMP, 1/68 (1.5%) were positive by the reference method and 6/68 (8.8%) by the developed method.ConclusionsThe developed method improved the sensitivity of ASFV detection from oral fluids and enabled early diagnosis of pigs before the onset of the disease. The developed method has the potential to enable simple and highly sensitive diagnosis of ASF, which is essential for its early diagnosis and effective surveillance.HighlightA method has been developed to detect ASFV in pig oral fluids with up to 100 times greater sensitivity than a reference method.The developed method combines innovative pretreatment in less than 60 min with conventional nucleic acid extraction with a column kit followed by real-time PCR or LAMP.Applied to feasibility assessment for ASF diagnosis on raised pigs, the developed method showed higher diagnostic sensitivity than the reference method.The developed method can be a useful tool that contributes to early detection and surveillance of ASFV.
Protein kinase Sut1 from the CrenarchaeonSulfolobus acidocaldariusdisplays tyrosine phosphorylation activity
Authors: Maklad Hassan R. , Ruys Sébastien Pyr dit , Bervoets Indra , Vertommen Didier , Gutierrez Gustavo J. , Vranken Wim F. , Siebers Bettina , Peeters Eveline 
Published: January 2024

AbstractProtein phosphorylation is a key cellular signaling mechanism that exists in all life forms. Unlike Bacteria and Eukarya, in which protein phosphorylation has thoroughly been studied, post-translational modification by means of phosphorylation have only been limitedly explored in Archaea. A previous study of the phosphoproteome of the model CrenarchaeonSulfolobus acidocaldariusrevealed a widespread occurrence of protein phosphorylation, especially on tyrosine residues. Moreover, several (putative) transcription factors, including AbfR1 and FadR, were previously shown to be phosphorylated on tyrosine residues, a phenomenon that is directly linked to a phosphorylation-mediated regulation of these transcription factors. Despite this potentially important role for tyrosine phosphorylation inS. acidocaldarius, to our knowledge, a kinase capable of directly phosphorylating tyrosine residues has not yet been identified in this organism, neither in Archaea as a whole. Here, we identify and characterize a protein kinase inS. acidocaldarius, Sut1, which displays tyrosine phosphorylation activityin vitroand represents a novel protein kinase family that is widespread in different archaeal organisms.
Biochemical characterization of theEscherichia colisurfaceome: A focus on type I fimbriae and flagella
Authors: Kavanaugh DW , Sivignon A , Rossez Y , Chouit Z , Chambon C , Beal L , Hébraud M , Guerardel Y , Nguyen H , Barnich N 
Published: January 2024

AbstractTheEscherichia colisurfaceome consists mainly of the large surface organelles expressed by the organism to navigate and interact with the surrounding environment. The current study focuses on type I fimbriae and flagella. These large polymeric surface organelles are composed of hundreds to thousands of subunits, with their large size often preventing them from being studied in their native form. Recent studies are accumulating which demonstrate the glycosylation of surface proteins or virulence factors in pathogens, includingE. coli.Using biochemical and glycobiological techniques, including biotin-hydrazide labelling of glycans and chemical and glycosidase treatments, we demonstrate i) the presence of a well-defined and chemically resistant FimA oligomer in several strains of pathogenic and non-pathogenicE. coli, ii) the major subunit of type I fimbriae, FimA, in pathogenic and laboratory strains is recognized by concanavalin A, iii) standard methods to removeN-glycans (PNGase F) or a broad-specificity mannosidase fail to remove the glycan structure, despite the treatments resulting in altered migration in SDS-PAGE, iv) PNGase F treatment results in a novel 32 kDa band recognized by anti-FliC antiserum.While the exact identity of the glycan(s) and their site of attachment currently elude detection by conventional glycomics/glycoproteomics, the current findings highlight a potential additional layer of complexity of the surface (glyco)proteome of the commensal or adhesive and invasiveE. colistrains studied.
Mechanistic Insights into G-protein Activation via Phosphorylation Mediated Non-Canonical Pathway
Authors: Shewani Kunal , Madhu Midhun K. , Murarka Rajesh K. 
Published: January 2024

AbstractActivation of heterotrimeric G-proteins (Gαβγ) downstream to receptor tyrosine kinases (RTKs) is a well-established crosstalk between the signaling pathways mediated by G-protein coupled receptors (GPCRs) and RTKs. While GPCR serves as a guanine exchange factor (GEF) in the canonical activation of Gαthat facilitates the exchange of GDP for GTP, the mechanism through which RTK phosphorylations induce Gαactivation remains unclear. Recent experimental studies revealed that the epidermal growth factor receptor (EGFR), a well-known RTK, phosphorylates the helical domain tyrosine residues Y154 and Y155 and accelerates the GDP release from the Gαi3, a subtype of Gα-protein. Using well-tempered metadynamics and extensive unbiased molecular dynamics simulations, we captured the GDP release event and identified the intermediates between bound and unbound states through Markov state models. The additional negative charges introduced by phosphorylations rewired the inter-residue interactions and significantly weakened the salt bridges at the domain interface, contributing to the increased separation of the Ras-like and helical domains of G-protein. Furthermore, the unfolding of helixαF resulted in greater flexibility near the hinge region, facilitating a greater distance between domains in the phosphorylated Gαi3. The release of GDP in the phosphorylated G-protein occurred at a faster rate compared to the unphosphorylated state, caused by increased fluctuations in conserved regions of P-loop, switch 1, and switch 2. Overall, this study provides atomistic insights into the activation of G-proteins induced by RTK phosphorylations and identifies the specific structural motifs involved in the process. The knowledge gained from the study could establish a foundation for targeting non-canonical signaling pathways and developing therapeutic strategies against the ailments associated with dysregulated G-protein signaling.
Fostering environmental preservation: exploring the synergy of green human resource management and corporate environmental ethics
Authors: Al-Swidi Abdullah Kaid , Al-Hakimi Mohammed A. , Al-Hattami Hamood Mohammed 
Published: January 2024

Purpose
This study aims to explore the unique and synergistic effects of green human resource management (GHRM) and corporate environmental ethics (CEE) on the environmental performance (EP) of manufacturing small and medium-sized enterprises (SMEs) in Yemen, a less developed country (LDC).

Design/methodology/approach
Through a cross-sectional survey design, data were collected from 262 manufacturing SMEs in Yemen and analyzed using “hierarchical regression analysis” via PROCESS Macro.

Findings
The empirical results showed that GHRM and CEE positively affect EP and, more importantly, that CEE and GHRM have a synergistic effect on EP.

Research limitations/implications
This study makes a theoretical contribution by integrating GHRM, CEE and EP into a single framework, taking into account the perspectives of the resource-based view and the ethical theory of organizing. The results corroborate the unique and synergistic effects of GHRM and CEE on EP of SMEs in the manufacturing sector.

Practical implications
The results of this study offer valuable insights for SME managers/decision-makers, who are anticipated to become more interested in integrating environmental ethics into their companies. This has implications that with the consideration of CEE, SMEs can benefit from GHRM practices to improve their EP.

Social implications
The study highlights the positive economic and social impact of SMEs adopting eco-friendly practices like GRHM. In today’s economy, it is not sufficient to simply strive for economic growth. It is possible for SMEs to achieve well-rounded performance by implementing the recommended framework that emphasizes the importance of social and environmental well-being.

Originality/value
This study advances the existing work on the impact of GHRM on EP by demonstrating the crucial role of CEE in predicting EP of manufacturing SMEs in LDCs like Yemen. Previous research on GHRM has mainly been conducted on SMEs in developed nations, which may not be entirely applicable to LDCs. It is crucial to understand this aspect in the context of LDCs so that SMEs can adopt environmental practices effectively in the future: how SMEs conserve the environment through their environmental practices.
DAY NRLM scheme and its impact on women empowerment: a case of Morigaon district of Assam, India
Authors: Ningombam Sanjeev Kumar , Bordoloi Sudeshna 
Published: January 2024

Purpose
This study aims to examine the political, economic, social and technological (PEST) factors of women empowerment in the context of rural development under the initiatives of the National Rural Livelihood Mission (NRLM).

Design/methodology/approach
The unit of analysis for this study is 459 samples. Non-probability sampling technique has been used for this study. Schedule/questionnaires have been circulated among the women in each block cluster. Frequencies have been used to represent the data against each statement. Non-parametric chi-square test has been used to examine the relationship between empowerment and political, economic, social, technological factors and interpersonal skills.

Findings
Statistical analysis shows that the Deendayal Antayodaya Yojana (DAY)-NRLM scheme has brought significant changes and development to women in political, social, economic and technological areas. It was observed that women are economically empowered and contribute to the economic upliftment of the family. Most of the respondents strongly felt that after being associated with DAY-NRLM, they were empowered to contribute efficiently to the social development process and activities.

Research limitations/implications
The findings of this study highlight the impact of DAY-NRLM implementation at block levels of a district in India. Subsequent research may be carried to measure the effectiveness of the capacity-building program conducted under the banner of DAY-NRLM.

Practical implications
Women empowerment is not limited to awareness, whereas it needs a complete set of initiatives backed with support systems. Few critical interventions could be building strong networks, enhancing financial management, encouraging the spirit of entrepreneurship among self-help group workers, providing easy access to credit, mentoring, handholding, continuous monitoring and evaluation.

Social implications
This study focuses on the impact of the DAY-NRLM scheme on women’s empowerment in the Morigaon district. The inferences from the study throw light on the empowerment of women vis-à-vis political, economic, social and technological factors.

Originality/value
This study is a primary study conducted in the Morigaon District of Assam. This is a new line of policy research that approaches the women empowerment with the PEST parameters.
Exchange rate and housing affordability in OECD countries
Authors: Salisu Afees Adebare , Rufai Aliyu Akorede , Nsonwu Modestus Chidi 
Published: January 2024

Purpose
This study aims to construct alternative models to establish the dynamic relationship between exchange rates and housing affordability by estimating both the short- and long-run relationship between exchange rates and housing affordability for 18 OECD countries from 1975Q1 to 2022Q4. After that, this study demonstrates how this nexus behaves during high and low inflation regimes and turbulent times.

Design/methodology/approach
This study uses the panel autoregressive distributed lag technique to examine the nexus between housing affordability to capture the distinct characteristics of the sample countries and estimate various short- and long-run dynamics in the relationship between housing affordability and exchange rate.

Findings
Exchange rate appreciation improves housing affordability in the short run, whereas this connection tends to dissipate in the long run. Moreover, inflation can worsen housing affordability during turbulent times, such as the global financial crisis, in both the short and long run. Ignoring these changes in the relationship between exchange rates and housing affordability during turbulent times can lead to incorrect conclusions.

Originality/value
To the best of the authors’ knowledge, this study is the first to examine the association between exchange rates and housing affordability by demonstrating how these variables behave in high and low inflation regimes and turbulent times.
Happiness at work: a systematic literature review
Authors: Farooq Foziya , Mohammad Sheikh Sajid , Nazir Nazir Ahmed , Shah Parvez Ahmad 
Published: January 2024

Purpose
This study aims to systematically review the literature on happiness at work (HAW) by analysing existing studies, identifying relevant themes in HAW research and evaluating the methodologies used in the literature.

Design/methodology/approach
The study used a systematic review process, following the guidelines and principles outlined in the updated Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement 2020 and checklist. Articles were collected from six databases: Emerald insight, Taylor & Francis Online, Science Direct, Wiley Online Library, Springer and MDPI. Subsequently, systematic review was performed on 41 HAW articles published in 29 different journals between 2010 and 2022. The authors only considered articles that were either indexed by Scopus or in the Academic Journal Guide (AJG) list.

Findings
The study identified six major themes, assessed the operationalisation of HAW and analysed the research methodologies and statistical tools used in the sample studies. Majority of the articles discussed the antecedents of HAW followed by the HAW as a mediator. There is a high heterogeneity in the operationalisation of HAW in the reviewed articles. Moreover, majority of the studies have prioritised service sectors over the industrial sectors.

Originality/value
This study represents the first comprehensive review of the existing literature on HAW by using a systematic review methodology.
Behavior of elastohydrodynamic lubricating film point contact problems in the unsteady state of reciprocating motion
Authors: Abd Alsamieh Mohamed 
Published: January 2024

Purpose
In this study a numerical analysis of the elastohydrodynamic lubrication point contact problem in the unsteady state of reciprocating motion is presented. The effects of frequency, stroke length and load on film thickness and pressure variation during one operating cycle are discussed. The general tribological behavior of elastohydrodynamic lubrication during reciprocating motion is explained.

Design/methodology/approach
The system of equations of Reynolds, film thickness considering surface deformation and load balance equations are solved using the Newton-Raphson technique with the Gauss-Seidel iteration method. Numerical solutions were performed with a sinusoidal contact surface velocity to simulate reciprocating elastohydrodynamics. The methodology is validated using historical experimental measurements/observations and numerical predictions from other researchers.

Findings
The numerical results showed that the change in oil film during a stroke is controlled by both wedge and squeeze effects. When the surface velocity is zero at the stroke end, the squeeze effect is most noticeable. As the frequency increases, the general trend of central and minimum film thickness increases. With the same entraining speed but different stroke lengths, the properties of the oil film differ from one another, with an increase in stroke length leading to a reduction in film thickness. Finally, the numerical results showed that the overall film thickness decreases with increasing load.

Originality/value
General tribological behaviors of elastohydrodynamic lubricating point contact, represented by pressure and film thickness variations over time and profiles, are analyzed under reciprocating motion during one working cycle to show the effects of frequency, stroke length and applied load.
Why Avoid Participating in an Accelerator? Countersignalling by High‐Quality FinTech Ventures
Authors: Browne Oliver , Cumming Douglas , Hutchinson Mark C. , Kirshner Samuel N. , O'Reilly Philip 
Published: January 2024

AbstractThis study investigates whether accelerators attract the highest‐performing FinTech ventures. Drawing on countersignalling theory, we present a model of accelerator participation that predicts high‐quality FinTech ventures will avoid accelerator programmes. Using a unique financial reporting dataset of FinTech accelerator participants and a propensity score‐matched sample of comparison firms, we find support for the prediction from our countersignalling model. FinTech accelerator participants have worse performance, both before and after completing a programme, when compared to peer non‐accelerator ventures. Overall, our results indicate that FinTech ventures are less suited for accelerators due to the current breadth of outside funding options available, which provides little incentive for participation from high‐quality ventures. This research has important implications for entrepreneurs, accelerators and investors.
Family ideology: uneasy entanglements of eldercare in Germany
Authors: Weiss Hadas 
Published: January 2024

AbstractMaterial pressures on privatized households are commonly absorbed through a family ideology, according to which members must pool resources and care for each other, while the family relations they thereby nurture are inherently valuable and constitute their own reward. Drawing on my fieldwork on family‐based eldercare in Germany, I explore the implications of this ideology. Specifically, I argue that it mollifies family caregivers’ unease about the injustices of Germany's eldercare system. Family members are entangled in this system as employers of mostly low‐paid, migrant caregivers; as performers of unremunerated care work at high personal costs; and, if they are women, as reproducers of a gendered division of care labour they object to and resent. Because this entanglement is filtered through the lens of family ideology, the focus of family caregivers shifts away from these broader injustices and towards co‐caregiver conflicts as well as expectations from and commitments towards other family members.
Inclusion in Russian schools: Exploring parents' perspectives
Authors: Malinina Irina 
Published: January 2024

AbstractAs schools in Russia become more inclusive for students with special educational needs (SEN), there is a scarcity of research on stakeholders' perspectives. Parents are one of the main actors in education, who possess valuable knowledge about their children and can represent their children's needs in a vicarious manner. This study gives voice to the parents of children with SEN and offers parents' perspectives on the liaison between families and schools. The article focuses on the experience of two families with children with special educational needs (namely, Autistic Spectrum Disorder and intellectual disability). Data was collected through semi‐structured interviews and questionnaires and analysed using the Interpretative Phenomenological Analysis (IPA). The main findings demonstrate that parents' expertise is sometimes neglected and marginalized for different reasons and the school system in Russia is rigid and not able to adjust its approaches to different learners as well as address and allay parents' concerns. Lack of direct interaction blocks the pathway for compromises and mutual understanding. In the parents' view, teachers act on their own assumptions which are oftentimes emotionally loaded and not clearly articulated for the parents. However, parents believe that all the actors of the educational process, that is, teachers, learners and parents, would benefit if teacher‐parent relationships involved a more sensitive and attentive attitude towards each other's requirements and wishes, providing opportunities for negotiating and discussing arguable issues. The paper demonstrates two cases of misunderstanding between teachers and parents and offers implications for practice in teacher education, policy‐making and social work.