bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal Relationship between Immune Cells and Gynecological Cancers through Bidirectional and Multivariable Mendelian Randomization Analyses
Authors: Zhang Yangyang , Lu Yangyuxiao , Wang Xuanyu , He Keren , Fang Mengqi , Xu Jiabao , Xu Ye , Tao Fangfang , Lü Ping 
Published: November 2023

Abstract
Background: Evidence suggests potential associations between gynecological malignancies and various immune cell chemicals and systems. However, establishing a causal relationship remains uncertain, as a comprehensive investigation into their interplay has yet to be undertaken.
Methods: We conducted a two-sample bidirectional Mendelian Randomization (MR) analysis to assess the potential link between immune cell traits and the risk of gynecological cancers, aiming to identify relevant factor. This work employed the inverse-variance weighted method (IVW) for multiple SNPs (Single Nucleotide Polymorphisms) or Wald ratio for one SNP to ascertain the causal association between exposure and outcome by utilizing genome-wide association study (GWAS) data on 731 immunophenotypes and gynecologic malignancies. Bidirectional two-sample MR and multivariable MR analyses were conducted to illustrate the causality. In addition, we use sensitivity analyses for assessment of the robustness of the results and colocalization for further validation.
Results: In IVW analysis, increases in relative counts of circulating CD11c+ HLA-DR++ monocyte was associated with an elevated risk of breast cancer (OR [95% CI], 1.1295 [1.0632-1.2000], P = 8.044 × 10-5), while elevated levels of HLA-DR on plasmacytoid DC and HLA-DR on DC cell were protective against breast cancer (OR [95% CI], 0.9541 [0.9324-0.9762], P = 5.876 × 10-5) (OR [95% CI], 0.9414, [0.9188-0.9646], P = 1.101 × 10-6). In addition, cell counts of CD39+ resting Treg and CD28+ CD45RA- CD8+ T cell were also shown to be causally associated with the development of ovarian and cervical cancer, respectively. Colocalization analysis showed the lead SNP, rs780094, suggesting HLA-DR GWAS shared a common genetic mechanism with breast cancer.
Conclusions: We identified a significant causal relationship between multiple immunophenotypes and breast cancer. Circulating immunophenotypes suggestive of breast cancer development can provide us with a basis for forecasting and predicting cancer.
Altered Regional Spontaneous Brain Activity and Default Mode Network Functional Connectivity in Herpes Zoster-related Pain Patients with High Anxiety
Authors: Zhou Li , Liu Qianhan , Liu Shengdan , Lu Xiaofei , Li Qian , He Yue , Ren Changhe , Chen Guangxiang 
Published: November 2023

Abstract
Pain and psychological disorders are two common symptom clusters in patients with herpes zoster-related pain (HZ-RP). Numerous neuroimaging studies have revealed abnormal brain structure and function in patients with HZ-RP. However, very few studies have focused on the functional characteristics of the brain in HZ-RP patients with different levels of anxiety. Fractional amplitude of low-frequency fluctuations (fALFF), regional homogeneity (ReHo) and functional connectivity (FC) were compared between HZ-RP patients with high anxiety (HZ-RP with HA n = 25) and HZ-RP patients with low anxiety (HZ-RP with LA, n = 26). Multivariable linear regression models were used to investigate the impact of clinical characteristics on the significant abnormalities, and moderation analysis was used to examine the interaction between pain and anxiety. The two groups showed significant differences in regional spontaneous brain activity in the right Rolandic operculum (RO), left middle temporal gyrus (MTG) and right precentral gyrus (PrCG). The left anterior prefrontal cortex showed significantly decreased FC with the left medial superior frontal gyrus while the left occipital cortex showed increased FC with the right fusiform, right MTG and left precuneus in the HZ-RP with HA group compared with that in the LA group. The degree of pain and anxiety contributed to explaining the variance in the fALFF of the right RO. However, the abnormal ReHo in the right PrCG was solely related to the degree of anxiety. The abnormal brain dysfunctional pattern may be an indicator of underlying pathophysiological mechanisms linking HZ-RP and anxiety. The right PrCG plays a vital role in regulating anxiety in HZ-RP patients.
One-hole split endoscope versus unilateral biportal endoscopy for lumbar spinal stenosis: a retrospective propensity score study
Authors: Li Tusheng , Jiang Qiang , Zhu Tengyue , Lu Zhengcao , Ding Yu 
Published: November 2023

Abstract

Background
The one-hole split endoscopy (OSE) was first proposed and clinically applied in China in 2019. The aim of this study was to compare the clinical efficacy of a one-hole split endoscopy (OSE) and unilateral biportal endoscopy (UBE) for treating lumbar spinal stenosis (LSS).
Methods
One hundred sixty patients with LSS who met the inclusion from November 2020 to August 2022 were analyzed and divided into OSE and UBE groups. The propensity score matching (PSM) method was used to adjust the imbalanced confounding variables between the two groups. After matching, surgical outcomes were recorded, and clinical data, including functional scores and imaging findings, were compared. Functional scores included the visual analog scale of leg pain (VAS-LP) and back pain (VAS-BP), the Japanese Orthopedic Association score (JOA), and the Oswestry Disability Index (ODI). Imaging data included dural sac cross-sectional area (DCSA), lumbar range of motion (ROM), and sagittal translation (ST).
Results
After PSM, 104 LSS patients were included in the study, and all covariates were well-balanced between the two groups. Among the matched patients, the OSE showed advantages over the UBE regarding operative time (62.42 ± 4.86 vs. 68.96 ± 4.56) and incision length (2.30 ± 0.14 vs. 2.70 ± 0.15) (P < 0.001). However, differences between the two groups in intraoperative blood loss, hospital length of stay, and complication rates were not statistically significant (P > 0.05). There was no statistically significant difference regarding VAS-BP, VAS-LP, JOA, and ODI between the two groups (P > 0.05). However, all clinical and functional scores significantly improved postoperatively (P < 0.05). Postoperative DCSA of both groups was significantly found to be improved (P < 0.05), ROM and ST remained within the normal range, and no cases of lumbar instability were recorded. According to the modified MacNab criteria, the excellent and reasonable rates in the OSE and UBE groups were 94.23% and 90.38%, respectively, with no statistically significant difference (P = 0.713).
Conclusion
Both OSE and UBE techniques for lumbar spinal stenosis provide satisfactory clinical outcomes. However, the OSE seems superior to the UBE technique regarding operation time and incision length.
MiR-493-3p inhibits proliferation and promotes differentiation of porcine muscle satellite cells through MKK7/JNK axis
Authors: Wu Lingling , Wang Zhaolu , Sun Xiaohui , Yang Jinjin , Jin Jianjun , Yang gongshe , Shi Xin’e 
Published: November 2023

Abstract

Background Skeletal muscle is one of the primary productions in pig industry, and the myogenesis represents one of the main economic traits of pig. MicroRNA (miRNA) plays critical roles in myogenesis of porcine, however, there are a large number of miRNAs that have not been studied in regulating myogenesis. Here, the effects of miR-493-3p on porcine myogenesis was examined. Results The miR-493-3p is strongly enriched in skeletal muscle, especially in the slow-twitch muscle fibers soleus. We further demonstrated that miR-493-3p significantly inhibits the proliferation of porcine primary muscle satellite cells (MSCs). In contrast, miR-493-3p significantly promotes the differentiation of porcine primary MSCs. Mechanistically, bioinformatic analysis and dual luciferase assay results show that the MAP kinase kinase 7 (MKK7) is a direct target of miR-493-3p. Moreover, miR-493-3p inhibits MKK7 expression, leading to the decreased phosphorylated level of JNK (p-JNK), thus regulate myogenesis. Conclusions Overall, these findings suggest that miR-493-3p inhibits proliferation and promotes differentiation of porcine MSCs via inhibiting MKK7/JNK axis. These finding helps to understand the development network of skeletal muscle regulated by miRNA, and provides a theoretical basis for improving pork production.
Acquisition and synchronisation of cardiography signals from a clinical patient monitor with facial video recordings
Authors: Meher Jagmohan , Wang Chien-Chih , Nordling Torbjörn E. M. 
Published: November 2023

Abstract

A far too frequent practical challenge in clinical informatics research and method development for acquiring vital signs is the extraction and synchronisation of signals from proprietary devices for the clinical monitoring of patients. In an ongoing study evaluating methods for video-based remote photoplethysmography (rPPG), we needed to extract ground truth values of electrocardiogram (ECG) and pulse oximetry (SpO2) signals from the Philips vitals monitor while recording the facial video of the subject, simultaneously. This ground truth data will be used to train the model that will perform rPPG. Various software can extract data from the Philips vitals monitor with features like data acquisition, parsing, and visualisation, but they lack synchronisation with the facial video. Therefore, we developed the Patient Monitor Data Extractor (PMDE), which collects data from the Philips IntelliVue monitors following the Data export interface programming guide provided by Philips. We set up a DHCP server on a Windows 7 computer with a webcam and interfaced with the monitor through LAN with UDP/IP. We used C++ and Windows Sockets API to develop our software and communicate over UDP. For synchronisation with the video cameras, we turned off the light in the room and used this sudden brightness drop as a trigger. The timestamp of the monitor was recorded when the webcam detected the trigger. The PMDE software records ECG at 500 Hz and SpO2 at 125 Hz with a synchronisation error of less than two sampling periods, which is about 40 ms for a 50 fps video. We conclude that PMDE is uniquely suited for recording data for rPPG evaluation because of its synchronisation feature. We have used PMDE to collect a dataset of facial videos with ground truth ECG and SpO2 signals. We intend to make PMDE available as open source to save other researchers time.
Governing COVID-19: The Complex Relationship Between Government Type and Police Enforcement
Authors: Bohloli Hamid , Shokri Azad , Khah Fahimeh Rabbani 
Published: November 2023

Abstract
Introduction: The COVID-19 pandemic is one of the most significant challenges to global health and economic and social aspects. This disease has spread to millions of people and caused hundreds of thousands of deaths worldwide. The COVID-19 pandemic not only posed unprecedented and unforeseen challenges to public health systems in the early 21st century but also created a set of challenges for police departments and law enforcement agencies worldwide.
Methods: We conducted a scoping review to map the existing literature on the relationship between government type and police enforcement in controlling COVID-19. We searched electronic databases such as PubMed, EMBASE, Web of Science, and Google Scholar. We included studies that were conducted in countries with different types of governance and published data on their police deployment in controlling COVID-19. We identified 18 countries that met our inclusion criteria. We used a four-quadrant composition model to plot the countries based on their government type and the extent of police deployment. We found a complex relationship among government type, police enforcement, and COVID-19 control.
Results: Based on the studies conducted and the literature review on the subject in various parts of the world, the four-zone model (Governance Policing Types - GPT) is proposed in accordance with the type of governance and the extent of police use in controlling COVID-19. This model indicates a clear relationship between the type of government and the extent of police use in controlling epidemiological crises. Research findings suggest that in pandemic emergencies, the type of governance and effective response of the police can keep individuals away from potential risks and ensure public safety and well-being.
Conclusion: Governance quality and police involvement are essential in managing pandemics; however, a balanced approach is needed to protect both public health and individual rights. Democratic countries generally have better decision quality, but authoritarian countries can make decisions more quickly. Countries that combined both quality and speed of decision-making achieved exceptional results. More research is needed to understand the long-term effects of different pandemic management strategies.
The cost-effectiveness of early COVID-19 detection using AI embedded wearable devices: Evidence from the COVID-RED Trial.
Authors: Korkmaz Serkan , Hansen Paul Lynggaard , Kjellberg Jakob 
Published: November 2023

Abstract
Trial Design: Randomized single-blinded two-period, two-sequence, crossover trial.
Methods
Objectives
Participants All residents of the Netherlands above 18 years of age who reads, understands and writes dutch. All eligible study participants who owns a smartphone, were recruited during the first quarter of 2021
Interventions All study participants recieved a bracelet which based on the dutch COVID-19 guidelines (control group), or the embedded AI (intervention group), warns the wearer of a possible COVID-19 infection.
Objective Estimate the time-difference in COVID-19 detection using AI, and the dutch COVID-19 guidelines.
Outcomes The incremental cost effectiveness ratio (ICER) to assess the costeffectiveness of using AI embedded bracelets.
Results
Number randomised 17,825 eligible study participants were randomized to the intervention group (N = 8,915) and control group (N = 8,910)
Recruitment The trial has ended, and is currently being studied.
Number analysed 2,952 and 2884 study participants opted out from the study in the intervention and control group respectively. The number of analysed study participants is 5,963 and 6,026 respectively.
Outcome COVID-19 can be detected 2 days earlier using AI embedded bracelets, than following the dutch COVID-19 guidelines. This comes at an additional cost of 0.85 e. The ICER is -0.42 (-0.47, -0.38) e.
Conclusions
COVID-19 can be detected 2 days earlier using AI embedded bracelets and comes at a low additional cost per study participant.
Trial registration Dutch Trial Register, NL930. Registered February 18, 2021, https://onderzoekmetmensen.nl/en/trial/23180
Funding Innovative Medicines Initiative (https://www.imi.europa.eu) 2 Joint Undertaking under grant agreement No 101005177.
Differential gene expression analysis reveals common biomarkers for systemic lupus erythematosus and atrial fibrillation
Authors: Wang Rongzi , Liu Juncheng , Zhang Tingting , Yao Siyu , Zhu Tingting , Yang Qichong , Ge Junbo , An Songtao 
Published: November 2023

Abstract
Background
Atrial fibrillation (AF) is a significant outcome of systemic lupus erythematosus (SLE), increasing the chances of experiencing blood clotting events and unforeseen mortality. As the underlying mechanism of SLE companied with AF is still unknown, this study sought to uncover potential biomarkers that could be of significant value for individuals dealing with SLE and AF, employing thorough bioinformatics research as the primary approach.
Methods
The NCBI Gene Expression Omnibus database (GEO) was employed to retrieve a collection of five microarray datasets (GSE50772, GSE41177, GSE79768, GSE81622, and GSE2240). By employing the online analytical tool GEO2R, we conducted an analysis of GSE50772 to pinpoint genes that exhibited differential expression. Significant module genes were discovered by WGCNA (weighted gene co-expression network analysis). To identify DEGs in AF, the 'Limma' package was utilized. Function of the common DEGs was found by functional enrichment analysis. The candidate biomarkers were discovered by applying a machine learning technique. The investigation involved the utilization of Single Sample Gene Set Enrichment Analysis (ssGSEA) scores to perform functional enrichment analysis on the identified candidate biomarkers. To predict the risk of AF in individuals with SLE, a nomogram and a ROC curve were created. The analysis focused on examining the presence of immune cells infiltrating the training datasets of SLE and AF, while also conducting a consensus cluster analysis specifically for SLE.
Results
29 common DEGs were identified between SLE and AF. The identification and utilization of five potential biomarkers-ANKRD36B, SLC4A4, ANKRD12, MTUS1 and DSC1-led to the creation of a nomogram with area under the receiver operating characteristic curve 0.900-0.981 across all datasets. The dysregulated immune cell infiltration was associated with the biomarkers. Based on the consensus clustering analysis, it was concluded that three subtypes were the most suitable in terms of quantity. The biomarkers exhibited different expression patterns among the subtypes. Regarding immunological infiltration, each subtype possessed unique traits.
Conclusion
By employing various bioinformatics research approaches and machine learning techniques, our study identified five candidate biomarkers (ANKRD36B, SLC4A4, ANKRD12, MTUS1, DSC1). Additionally, a nomogram capable of predicting the likelihood of both SLE and AF was developed. The results of our study provide a foundation for future investigations on potential important genes for AF in individuals with SLE. Moreover, it was discovered that AF and SLE exhibited abnormal compositions of immune cells.
Effects of different hinge positions and coronal correction angle on tibial rotation in uniplanar medial opening wedge high tibial osteotomy with three-dimensional tibial models
Authors: Jing Lizhong , Chen Yanrong , Wang Weiguo , Zhang Xia , Yang Jiushan , Wang Jian 
Published: November 2023

Abstract

Purpose: There is a lack of studies investigating the distal tibial rotation (DTR) during medial opening wedge high tibial osteotomy (MOWHTO). The purpose of the present study was to evaluate the impacts of different hinge positions in the sagittal and axial planes and coronal correction angle on the axial rotation of tibia in uniplanar MOWHTO with three-dimensional tibial models.
Methods: Preoperative CT data from 30 knee joints in 30 patients who underwent surgery for varus malalignment of knee were included. 1 standard hinge position (0°), 6 axial planes (±5°, ±10°, ±15°), and 6 sagittal planes (±5°, ±10°, ±15°) hinge positions were defined and virtual osteotomy was performed. The correction angle of each model was generated using Fujisawa’s point. The correction angle, preoperative medial proximal tibial angle, and DTR were measured.
Results: A significant linear correlation was observed between changes in hinge position in the sagittal plane and DTR. The changes in DTR were the smallest when the hinge position was at 5°, where internal or external rotation of the DTR may occur. When the front aspect of hinge axis rotated distally, DTR tended towards internal. Meanwhile, when the front aspect of hinge axis rotated proximally, DTR tended towards external. There were no correlations with every hinge axis position in the axial plane. There were linear correlations between the correction angle and DTR in varying degrees dependent on different hinge positions.
Conclusions: Both hinge position and coronal correction angle resulted in tibial rotation in uniplanar MOWHTO. In the sagittal plane, every change in hinge position was significantly linearly correlated with tibial rotation. However, no linear correlations were observed between every hinge position change in the axial plane. In addition, there were linear correlations between the coronal correction angle and tibial rotation in varying degrees dependent on the different hinge positions.
Research on landscape quality evaluations of rural roads using computer vision
Authors: Shen Zhigang , Tang Xiangong , He Xunyang , Wang Kelin 
Published: November 2023

Abstract

Rural roads are important channels connecting residents' lives and production, and their landscape quality affects the appearance of local villages. However, few existing rural studies have investigated rural road landscapes, and there is a lack of a quantitative interpretation system for the visual quality of landscapes. This study aimed to use a set of images to train a semantic segmentation model for rural road landscapes based on computer vision technology, and establish a quantifiable landscape-feature index system. The physical features of a landscape were extracted from panoramic photos of Chunhua Village in Changsha City, Hunan Province, using a semantic image segmentation algorithm, and the relationship between the quantified landscape features and visual perception was explored using multiple linear regression models. It was found that the trained semantic segmentation model of rural-community street-landscape images reached an accuracy of 0.83. The results of the linear regression model showed that the green vision index (GVI), farmland visibility index (FVI), building density index (BDI), and road width index (RWI) had significant effects on visual perception. The GVI and FVI were positively correlated, while the BD and RWI were negatively correlated. In the planning of rural road landscapes, emphasis should be placed on improving the proportion and quality of the natural landscape, and it is necessary to weigh the practicality and appreciation of man-made landscape elements. Finally, this paper provides a research method based on computer vision technology for the study of rural road landscapes, and provides a reference for the quantitative analysis of large-scale rural landscapes.
Growth-dependent Cr(VI) reduction by Alteromonas sp. under haloalkaline conditions: toxicity, removal mechanism and effect of heavy metals
Authors: Reddy G. Kiran Kumar , Kavibharathi K. , Singh Anuroop , Nancharaiah Y. V. 
Published: November 2023

Abstract
Bacterial reduction of hexavalent chromium (Cr(VI)) to trivalent chromium (Cr(III)) is a sustainable bioremediation approach. However, Cr(VI) by bacteria is severely impeded by Cr(VI) toxicity and complex environmental conditions like salt, alkaline pH and heavy metals. Hence, there is a need for Cr(VI) reducing bacteria to thrive as well as to metabolize under complex conditions. This study investigated Cr(VI) reduction, toxicity and removal mechanisms under complex conditions using an Alteromonas sp. isolated from the aerobic granular sludge cultivated from seawater-borne microorganisms. Rapid and complete removal of 100 mg/L Cr(VI) was achieved within 24 h under haloalkaline conditions (salinity: 3.5 to 7.5; pH 8 to 11). This strain exhibited high tolerance to heavy metals under haloalkaline conditions and reduced 100 mg/l Cr(VI) within 24 h in the presence of 100 mg/L As(V), 100 mg/L Pb(II), 50 mg/L Cu(II) or 5 mg/L Cd(II). The toxicity of Cr(VI) on the bacterial cells was evident by the increased reactive oxygen species levels and inhibition of esterase activity. Regardless of Cr(VI) toxicity, the cells grew and efficiently reduced Cr(VI) to Cr(III). The bacterial Cr(VI) reduction was strongly dependent on the growth, necessitating actively growing cells and growth medium. While, resting cells and spent medium barely contributed to Cr(VI) reduction. The biochemical assays revealed efficient Cr(VI) reduction using a cytosolic protein fraction from Alteromonas sp. and an exogenous reducing agent (e.g., NADPH). This study demonstrates an efficient Cr(VI) reduction system for potential Cr(VI) bioremediation applications under complex conditions including extreme haloalkaline conditions and toxic heavy metals.