bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sixteen issid planthopper species in one day in Dong Son-Ky Thuong Nature Reserve in North Vietnam: Eight new species, one new genus and additional new records (Hemiptera: Fulgoromorpha: Issidae)
Authors: Constant Jérôme , Pham Hong Thai 
Published: January 2024

Sixteen species of Issidae (Hemiptera: Fulgoromorpha) were recorded in a single day of collecting in Dong Son-Ky Thuong Nature Reserve, Quang Ninh Province, Vietnam, of which a checklist is provided. Among them, eight species are new to science and described in different tribes and subtribes: in the Hemisphaeriini Hemisphaeriina: Gergithoides olivaceus sp. nov., Hemisphaerius bresseeli sp. nov. and Neogergithoides scapularis sp. nov.; Hemisphaeriini Mongolianina: Mongoliana vietnamica sp. nov.; Kodaianellini: Kodaianella mua sp. nov.; Parahiraciini Parahiraciina: Pusulissus quangninhensis sp. nov. and Rostrolatum curviceps sp. nov.; Sarimini: Pseudocoruncanius obliquus sp. nov. The new genus Melichergithus gen. nov. is described to accommodate Gergithus gravidus Melichar, 1906 and the new combination Melichergithus gravidus (Melichar, 1906) gen. et comb. nov. is proposed; a black form of the species is recorded for the first time and described. Two species, Longieusarima lunulia Wang, Bourgoin & Zhang, 2017 and Parallelissus fuscus Meng, Qin & Wang, 2020, are recorded from Vietnam for the first time, while another species Maculergithus luteomaculatus (Constant & Pham, 2016) is recorded for the first time from Quang Ninh Province. Four species were represented by single females that could not be identified to species level, belonging to the genera Clypeosmilus Gnezdilov & Soulier-Perkins, 2017, Fortunia Distant, 1909 (two species) and Kodaianellissus Wang, Bourgoin & Zhang, 2017. The five following genera are recorded from Vietnam for the first time: Kodaianella Fennah, 1956, Rostrolatum Che, Zhang & Wang, 2020, Longieusarima Wang, Bourgoin & Zhang, 2017, Parallelissus Meng, Qin & Wang, 2020 and Kodaianellissus. The type material of Hemisphaerius rufovarius Walker, 1858 and its junior synonyms H. scymnoides Walker, 1862, H. testaceus Distant, 1906 and H. virescens Distant, 1906 was studied and the male terminalia described; as a result, H. rufovarius is removed from the fauna of Vietnam and China. Hemisphaerius bipunctatus Melichar, 1906 is also removed from the fauna of Vietnam after examination of the corresponding specimens. This study provides a 25% increase in the number of Issidae of Vietnam; a complete list of the 51 species recorded from the country, as well as a map of the number of recorded species per province, are given and discussed.
L’apport de la génétique pour comprendre la colonisation de l’île Tomé (Côtes-d’Armor, France) par le Vison d’Amérique, Mustela vison Schreber, 1777 : conséquences pour sa gestion
Authors: Lorvelec Olivier , Riallin Stéphane , Boisson Pierre-Yves , Bredin Maxime , Deniau Armel , Dutouquet Louis , Guiguen Stéphane , Hervé Quentin Le , Quilliec Patricia Le , Primas Olivier , Provost Pascal , Petit Éric J. 
Published: January 2024

La première observation confirmée de Mustela vison Schreber, 1777, le Vison d’Amérique, sur l’île Tomé (département des Côtes-d’Armor, Bretagne, France) date de 2012. Depuis, un programme d’action intitulé « Trégor Gestion Vison », réunissant les structures locales impliquées dans les problématiques environnementales, a été mis en place pour essayer d’éradiquer l’espèce sur cette île. Ce programme a permis de collecter, entre 2014 et 2020, du matériel biologique provenant de l’île Tomé (34 échantillons) mais également du continent proche (Côtes-d’Armor, 38 échantillons). Une étude génétique a été conduite, à partir de ce matériel biologique, afin d’identifier les modalités de la colonisation de l’île Tomé. À cet effet, l’ensemble des échantillons a été génotypé à 16 marqueurs microsatellites. Les résultats montrent que l’île Tomé n’est pas isolée du continent proche, dans la mesure où au moins cinq individus sont considérés comme des immigrants. Il est donc cohérent de penser qu’il y a en moyenne un individu qui colonise l’île chaque année. La mise en évidence de plusieurs événements de colonisation indique également que la colonisation de l’île se fait naturellement et qu’il ne peut s’agir d’une introduction délibérée. Ces résultats expliquent également pourquoi, après une éradication a priori réussie en 2018, l’île a de nouveau hébergé des visons à partir de 2020. Cette étude génétique apporte des résultats pouvant aider les gestionnaires dans la mise en place d’une stratégie de limitation des effectifs de visons présents sur l’île Tomé.
“Normalising” flying-foxes: a bold vision for improving the public perceptions of our largest and most conspicuous bats
Authors: Mo Matthew , Timmiss Libby A. , Pearson Tim , Kerr Maree Treadwell , Steves Sera , Welbergen Justin A. 
Published: January 2024

ABSTRACT
Negative public attitudes toward particular species can have significant impact on their conservation. Traditional approaches to improving public perceptions of flying-foxes include defending them, encouraging co-existence with them and promoting empathy for them following publicised mortality events. We propose a shift toward “normalising” flying-foxes, in which they are portrayed as interesting species and a natural part of our highly esteemed biodiversity. This has somewhat already begun in a piecemeal fashion, and we provide examples of flying-foxes being represented in a range of formats, including museum and photography exhibitions, public artwork and street décor, books, preschool and school education, wildlife-based tourism and community events. However, there remains paucity in flying-foxes being represented alongside portrayals of Australian wildlife at a broader scale. We suggest that proponents fearing public backlash or impacts on market responses are a likely barrier to seeing flying-foxes being represented more widely; but conclude with hope that current participation in depicting flying-foxes as interesting and part of biodiversity will gain momentum. Ultimately, through normalising flying-foxes, public attitudes adopting their intrinsic value may eventually overshadow the perspectives of flying-foxes connected to contentious issues.
Impact of Seed Priming and Growing Media on Germination and Seedling Growth of Bitter Gourd
Authors: Khatun Most. Shamima , Islam Nazrul , Choudhury Shormin 
Published: January 2024

Aims: The aim of this study was to determine the suitable growing media and priming treatments for seed germination and seedling growth of bitter gourd (cv. BARI hybrid Korola 2).
Study Design: The experiment was set up in a completely randomized design (CRD) with sixteen treatments, each containing fifteen seedlings.
Place and Duration of the Study: The experiment was conducted on Horticulture farm at Sher-e-Bangla Agricultural University; Dhaka, Bangladesh from March to April 2021.
Methodology: Four seed priming treatments were used in the experiments: T0: Seed soak in distilled water for 12 h (control), T1:Hot water treatment (450C for 5 min), T2: Poly ethylene glycol (PEG) 6000 (5%), T3: Sodium chloride solution (NaCl) (2%) soak for 12h and four growing media viz.,M0: soil + cow dung (1:1), M1: soil + cow dung + vermicompost (1:1:1), M2: soil + cow dung + sawdust (1:1:1), M3: soil + cow dung + cocopeat (1:1:1).
Results: The maximum germination percentage (100%), highest shoot (21.00 cm) and root length (12.83 cm), seed vigor index (3383.3) was recorded in T2M3 treatment. However, the maximum photosynthetic pigment (30.31 mg/g), relative water content (96.99%) and shoot (3.53g) and root fresh weight (0.47 g) was found in T2M1 treatment.
Conclusion: It can be concluded that soil + cow dung + cocopeat (1:1:1) with PEG 6000 (5%) is the best treatment for bitter gourd seed germination, while soil + cow dung + vermicompost (1:1:1) with PEG 6000 (5%) is better for vigor and healthy seedlings.
PKB/Akt phosphorylates the CDC25B phosphatase and regulates its intracellular localisation
Authors: Baldin Véronique , Theis‐Febvre Nathalie , Benne Clarisse , Froment Carine , Cazales Martine , Burlet‐Schiltz Odile , Ducommun Bernard 
Published: October 2003

AbstractRegulation of the intracellular localisation of its actors is one of the key mechanisms underlying cell cycle control. CDC25 phosphatases are activators of Cyclin‐Dependent Kinases (CDK) that undergo nucleo‐cytoplasmic shuttling during the cell cycle and in response to checkpoint activation. Here we report that the protein kinase PKB/Akt phosphorylates CDC25B on serine 353, resulting in a nuclear export‐dependent cytoplasmic accumulation of the phosphatase. Oxidative stress activates PKB/Akt and reproduces the effect on CDC25B phosphorylation and localisation. However, inhibition of PKB/Akt activity only partially reverted the effect of oxidative stress on CDC25B localisation and mutation of serine 353 abolishes phosphorylation but only delays nuclear exclusion. These results indicate that additional mechanisms are also involved in preventing nuclear import of CDC25B. Our findings identify CDC25B as a target of PKB/Akt and provide new insight into the regulation of its localisation in response to stress‐activated signalling pathways.
Functional Expression of a Bacterial Heavy Metal Transporter in Arabidopsis Enhances Resistance to and Decreases Uptake of Heavy Metals
Authors: Lee Joohyun , Bae Hyunju , Jeong Jeeyon , Lee Jae-Yun , Yang Young-Yell , Hwang Inhwan , Martinoia Enrico , Lee Youngsook 
Published: October 2003

AbstractLarge parts of agricultural soil are contaminated with lead (Pb) and cadmium (Cd). Although most environments are not heavily contaminated, the low levels observed nonetheless pose a high risk of heavy metal accumulation in the food chain. Therefore, approaches to develop plants with reduced heavy metal uptake are important. Recently, many transgenic plants with increased heavy metal resistance and uptake of heavy metals were developed for the purpose of phytoremediation. However, to reduce heavy metal in the food chain, plants that transfer less heavy metals to the shoot are required. We tested whether an Escherichia coli gene, ZntA, which encodes a Pb(II)/Cd(II)/Zn(II) pump, could be useful for developing plants with reduced heavy metal content. Yeast cells transformed with this gene had improved resistance to Pb(II) and Cd(II). In Arabidopsis plants transformed with ZntA, ZntA was localized at the plasma membrane and improved the resistance of the plants to Pb(II) and Cd(II). The shoots of the transgenic plants had decreased Pb and Cd content. Moreover, the transgenic protoplasts showed lower accumulation of Cd and faster release of preloaded Cd than wild-type protoplasts. These results show that a bacterial transporter gene, ZntA, can be functionally expressed in plant cells, and that that it may be useful for the development of crop plants that are safe from heavy metal contamination.
Topical, single dose bisphosphonate treatment reduced bone resorption in a rat model for prosthetic loosening
Authors: Åstrand Jörgen , Aspenberg Per 
Published: October 2003

AbstractFluid pressure, instability or particles have been suggested to cause peri‐prosthetic bone resorption. High intracapsular pressures have been reported in hip joints with loose prosthetic components, and oscillating fluid pressure has been shown to cause dramatic bone resorption in animal models. Resorption can be reduced by systemic bisphosphonate treatment in rat models with oscillating fluid pressure, but this has required higher doses than needed to inhibit normal remodelling. Bisphosphonates have high affinity to bone mineral. Topical application of the drug is therefore feasible. We used a previously described rat model where oscillating fluid pressure causes bone resorption. Before pressurization, a 1 mg/ml solution of alendronate was applied onto the bone surface for 1 min, after which excess bisphosphonate was rinsed away. Bone resorption was measured on histological slides as soft tissue area at the interface. Rats treated with topical alendronate had soft tissue areas reduced by half. Topical bisphosphonate treatment before cementing a joint implant could possibly reduce the risk of later loosening. © 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.
Vascular endothelial growth factor 121 and 165 in the subacromial bursa are involved in shoulder joint contracture in type II diabetics with rotator cuff disease
Authors: Handa Akiyoshi , Gotoh Masafumi , Hamada Kazutoshi , Yanagisawa Kazuhiro , Yamazaki Hitoshi , Nakamura Masato , Ueyama Yoshito , Mochida Joji , Fukuda Hiroaki 
Published: October 2003

AbstractVascular endothelial growth factor (VEGF) is a glycoprotein that plays an important role in neovascularization and increases vascular permeability. We reported that VEGF is involved in motion pain of patients with rotator cuff disease by causing synovial proliferation in the subacromial bursa (SAB). The present study investigates whether VEGF is also involved in the development of shoulder contracture in diabetics with rotator cuff disease. We examined 67 patients with rotator cuff disease, including 36 with complete cuff tears, 20 with incomplete tears, and 11 without apparent tears (subacromial bursitis). The patients were into groups according to the presence or absence of diabetes (14 type II diabetics and 53 non‐diabetics). Specimens of the synovium of the SAB were obtained from all patients during surgery. Expression of the VEGF gene in the synovium of the subacromial bursa was evaluated by using the reverse transcriptase polymerase chain reaction. The VEGF protein was localized by immunohistochemistry, and the number of vessels was evaluated based on CD34 immunoreactivity. The results showed that VEGF mRNA was expressed in significantly more diabetics (100%, 14/14) than in non‐diabetics (70%, 37/53) (P = 0.0159, Fisher's test). Investigation of VEGF isoform expression revealed VEGF121 in all 14 diabetics and in 37 of the 53 non‐diabetics, VEGF 165 in 12 of the 14 diabetics and in 21 of the 53 non‐diabetics, and VEGF 189 in 1 of the 14 diabetics and in 2 of the 53 non‐diabetics. No VEGF206 was expressed in either group. VEGF protein was localized in both vascular endothelial cells and synovial lining cells. The mean number of VEGF‐positive vessels and the vessel area were also significantly greater in the diabetics (p < 0.015, Mann‐Whitney U test). Synovial proliferation and shoulder joint contracture were more common in the diabetics (P = 0.0329 and P = 0.073, respectively; Fisher's test). The mean preoperative range of shoulder motion significantly differed in terms of elevation between two groups: 103.8° in diabetics and 124.9° in no diabetics (p = 0.0039 Mann–Whitney U test). In contrast, external rotation did not significantly differ: 44° in diabetics and 49° in non‐diabetics (p ° 0.4957, Mann–Whitney U test). These results suggest that VEGF121 and VEGF165 expression in the SAB is responsible for the development of shoulder joint contracture, especially in elevation, among type II diabetic patients with rotator cuff disease. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
Specificity of muscle action after anterior cruciate ligament injury
Authors: Williams Glenn N. , Barrance Peter J. , Snyder‐Mackler Lynn , Axe Michael J. , Buchanan Thomas S. 
Published: October 2003

AbstractNeuromuscular control is believed to be a critical factor in dynamic knee stability. The purpose of this study was to evaluate voluntary muscle control in anterior cruciate ligament deficient (ACL‐D) and uninjured people. Twenty athletes of similar age participated in this study. Subjects performed a target‐matching protocol that required them to produce isometric moments about the knee with fine control in flexion, extension, varus, and valgus (i.e., loads were generated in the plane perpendicular to the long axis of the shank). Electromyographic data were collected from 10 muscles that span the knee. A specificity index was calculated for each muscle to describe how fine‐tuned (specific) its muscle activity pattern was with respect to its principal direction of action in the load plane. Diminished specificity of muscle action was observed in 8 of 10 muscles in the ACL‐D subjects' involved knees when compared with the activity patterns from their uninvolved knees and those from the uninjured subjects' knees. The vastus lateralis muscle was especially affected. Increased and more global co‐contraction was also observed in the ACL‐D limbs. The alterations in muscle firing patterns observed in this study are consistent with diminished neuromuscular control. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
The effects of sex and estrogen therapy on bone ingrowth into porous coated implant
Authors: Shih Lih‐Yuann , Shih Hsin‐Nung , Chen Tain‐Hsiung 
Published: October 2003

AbstractAmounts of bone ingrowth into porous cobalt‐chromium plugs were compared between male and female dogs, and among sham‐operated and ovariectomized female dogs, with or without estrogen treatment, to investigate the effect of gender and estrogen therapy on biologic fixation. Each group consisted of eight skeletally mature dogs. Plugs were implanted bilaterally in the distal femur at 6 months after ovariectomy or sham operation. Estrogen treatment group received estradiol 20 μg/kg/day subcutaneous injection. Three months after implantation, histological examination showed significantly more bone ingrowth in areas with cortical bone contact than in areas with cancellous bone contact (P < 0.001 for all groups). Bone ingrowth was essentially the same in male and female control dogs. Ovariectomized dogs showed less overall bone ingrowth than male and female controls (P = 0.007). Bone ingrowth in areas with cortical bone contact did not decrease significantly, whereas bone ingrowth in areas with cancellous bone contact was significantly impaired (P < 0.001) in ovariectomized dogs compared with female controls. Short‐term, high‐dose estradiol treatment did not increase bone ingrowth volume fraction. Mechanical tests did not show any statistical differences among groups.Conclusion: Type of bone contact is the key factor affecting the amount and pattern of bone ingrowth into the porous surface. Ovariectomy results in decreased bone ingrowth in areas with cancellous bone contact, but does not compromise bone ingrowth in areas with cortical bone contact. Short‐term, high‐dose estradiol treatment does not enhance bone ingrowth into the porous surface. Extensively coated or full‐coated porous prostheses are recommended to achieve enough cortical bone contact and ingrowth for postmenopausal patients. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
Standardization of bluefin tuna, Thunnus thynnus, catch per unit effort in the baitboat fishery of the Bay of Biscay (Eastern Atlantic)
Authors: Rodríguez-Marín E. , Arrizabalaga H. , Ortiz M. , Rodríguez-Cabello C. , Moreno G. , Kell L.T. 
Published: October 2003

AbstractRelative indices of abundance by age class for bluefin tuna from 1975 to 2000 were estimated using generalized linear mixed models. Age class was included as a fixed factor within the model specifications, with the Year×Age interaction as a fixed factor component in order to obtain annual indices by age. Catch and effort data on bluefin tuna were available from two sources, catches by trip, and daily catches from logbooks. Catches were modelled using the delta-lognormal model. The model finally selected included the following explanatory factors: Year, Age, Year×Age, Month, number of Crew, number of Bait Tanks, and with the Year×Month interaction as a random component. Overall, year trends and coefficients of variation were similar from both datasets by age class. In general, the standardization procedure showed that vessel characteristics and technological advances related to fishing have a relatively minor explanatory effect on the observed catch rates of bluefin tuna in the fishery. Neither geographical distribution nor type of bait had an explanatory effect on the observed catch rates. The 1994 cohort stands out as an exceptionally large year class; it can be followed through the standardized series. This standardized catch rate index at age of bluefin tuna is the most complete yet available and is the only one currently used for juvenile fish in calibrating population models to evaluate the Eastern Atlantic and Mediterranean stock. It is particularly valuable because, apart from improvements in electronic technology, there are no changes in the method of exploitation during the entire series.
Diurnal and Light-Regulated Expression of AtSTP1 in Guard Cells of Arabidopsis
Authors: Stadler Ruth , Büttner Michael , Ache Peter , Hedrich Rainer , Ivashikina Natalya , Melzer Michael , Shearson Sarah M. , Smith Steven M. , Sauer Norbert 
Published: October 2003

Abstract
Guard cell chloroplasts are unable to perform significant photosynthetic CO2 fixation via Rubisco. Therefore, guard cells depend on carbon supply from adjacent cells even during the light period. Due to their reversible turgor changes, this import cannot be mediated by plasmodesmata. Nevertheless, guard cells of several plants were shown to use extracellular sugars or to accumulate sucrose as an osmoticum that drives water influx to increase stomatal aperture. This paper describes the first localization of a guard cell-specific Arabidopsis sugar transporter involved in carbon acquisition of these symplastically isolated cells. Expression of the AtSTP1 H+-monosacharide symporter gene in guard cells was demonstrated by in situ hybridization and by immunolocalization with an AtSTP1-specific antiserum. Additional RNase protection analyses revealed a strong increase of AtSTP1 expression in the dark and a transient, diurnally regulated increase during the photoperiod around midday. This transient increase in AtSTP1 expression correlates in time with the described guard cell-specific accumulation of sucrose. Our data suggest a function of AtSTP1 in monosaccharide import into guard cells during the night and a possible role in osmoregulation during the day.