bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contemporary preclinical mouse models for pediatric rhabdomyosarcoma: from bedside to bench to bedside
Authors: Martynov Illya , Dhaka Lajwanti , Wilke Benedikt , Hoyer Paul , Vahdad M. Reza , Seitz Guido 
Published: February 2024

BackgroundRhabdomyosarcoma (RMS) is the most common pediatric soft-tissue malignancy, characterized by high clinicalopathological and molecular heterogeneity. Preclinical in vivo models are essential for advancing our understanding of RMS oncobiology and developing novel treatment strategies. However, the diversity of scholarly data on preclinical RMS studies may challenge scientists and clinicians. Hence, we performed a systematic literature survey of contemporary RMS mouse models to characterize their phenotypes and assess their translational relevance.MethodsWe identified papers published between 01/07/2018 and 01/07/2023 by searching PubMed and Web of Science databases.ResultsOut of 713 records screened, 118 studies (26.9%) were included in the qualitative synthesis. Cell line-derived xenografts (CDX) were the most commonly utilized (n = 75, 63.6%), followed by patient-derived xenografts (PDX) and syngeneic models, each accounting for 11.9% (n = 14), and genetically engineered mouse models (GEMM) (n = 7, 5.9%). Combinations of different model categories were reported in 5.9% (n = 7) of studies. One study employed a virus-induced RMS model. Overall, 40.0% (n = 30) of the studies utilizing CDX models established alveolar RMS (aRMS), while 38.7% (n = 29) were embryonal phenotypes (eRMS). There were 20.0% (n = 15) of studies that involved a combination of both aRMS and eRMS subtypes. In one study (1.3%), the RMS phenotype was spindle cell/sclerosing. Subcutaneous xenografts (n = 66, 55.9%) were more frequently used compared to orthotopic models (n = 29, 24.6%). Notably, none of the employed cell lines were derived from primary untreated tumors. Only a minority of studies investigated disseminated RMS phenotypes (n = 16, 13.6%). The utilization areas of RMS models included testing drugs (n = 64, 54.2%), studying tumorigenesis (n = 56, 47.5%), tumor modeling (n = 19, 16.1%), imaging (n = 9, 7.6%), radiotherapy (n = 6, 5.1%), long-term effects related to radiotherapy (n = 3, 2.5%), and investigating biomarkers (n = 1, 0.8%). Notably, no preclinical studies focused on surgery.ConclusionsThis up-to-date review highlights the need for mouse models with dissemination phenotypes and cell lines from primary untreated tumors. Furthermore, efforts should be directed towards underexplored areas such as surgery, radiotherapy, and biomarkers.
Modeling the SDF-1/CXCR4 protein using advanced artificial intelligence and antagonist screening for Japanese anchovy
Authors: Yahiro Issei , Barnuevo Kyle Dominic Eguid , Sato Oga , Mohapatra Sipra , Toyoda Atsushi , Itoh Takehiko , Ohno Kaoru , Matsuyama Michiya , Chakraborty Tapas , Ohta Kohei 
Published: February 2024

SDF-1/CXCR4 chemokine signaling are indispensable for cell migration, especially the Primordial Germ Cell (PGC) migration towards the gonadal ridge during early development. We earlier found that this signaling is largely conserved in the Japanese anchovy (Engraulis japonicus, EJ), and a mere treatment of CXCR4 antagonist, AMD3100, leads to germ cell depletion and thereafter gonad sterilization. However, the effect of AMD3100 was limited. So, in this research, we scouted for CXCR4 antagonist with higher potency by employing advanced artificial intelligence deep learning-based computer simulations. Three potential candidates, AMD3465, WZ811, and LY2510924, were selected and in vivo validation was conducted using Japanese anchovy embryos. We found that seven transmembrane motif of EJ CXCR4a and EJ CXCR4b were extremely similar with human homolog while the CXCR4 chemokine receptor N terminal (PF12109, essential for SDF-1 binding) was missing in EJ CXCR4b. 3D protein analysis and cavity search predicted the cavity in EJ CXCR4a to be five times larger (6,307 Å³) than that in EJ CXCR4b (1,241 Å³). Docking analysis demonstrated lower binding energy of AMD3100 and AMD3465 to EJ CXCR4a (Vina score −9.6) and EJ CXCR4b (Vina score −8.8), respectively. Furthermore, we observed significant PGC mismigration in microinjected AMD3465 treated groups at 10, 100 and 1 × 105 nM concentration in 48 h post fertilized embryos. The other three antagonists showed various degrees of PGC dispersion, but no significant effect compared to their solvent control at tested concentrations was observed. Cumulatively, our results suggests that AMD3645 might be a better candidate for abnormal PGC migration in Japanese anchovy and warrants further investigation.
Impact of cognitive reserve on bipolar disorder: a systematic review
Authors: Matsumoto Kazuki , Hamatani Sayo 
Published: February 2024

BackgroundCognitive reserve (CR) is a complex concept that includes premorbid IQ, years of education, and exposure to neuropsychological stimuli through work and leisure. Previous studies have suggested that CR has a positive impact on several aspects of bipolar disorder. Synthesizing the evidence to date is an important work in providing directions for future studies. The objectives of this systematic review to summary impact of CR on onsetting, relapsing bipolar episodes, buffering cognitive dysfunctions, and maintaining quality of life (QOL) in bipolar disorder.MethodsTwo researchers independently reviewed selected paper from three database as PubMed, PsychINFO, and Web of Science. The search keywords were “bipolar disorder” and “cognitive reserve.” The selected studies were classified as the levels of evidence according to the criteria of the Oxford Center for Evidence- Based Medicine. The results of the selected studies were summarized according to the objectives.ResultsThrity six studies were included in this review. People with high CR may have fewer bipolar episodes and alleviate cognitive impairments and dysfunction. CR may keep the functional level in patients with bipolar disorder.ConclusionThe results of this systematic review suggest that CR may be involved in preventing relapse of bipolar episodes and may alleviate cognitive dysfunction. However, effect on prevention of onset-risk and relapse of bipolar episodes need further investigation in prospective studies.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021270293, the protocol was registered with PROSERO (CRD42021270293).
Electroacupuncture versus Escitalopram for mild to moderate Post-Stroke Depression: A randomized non-inferiority trial
Authors: Ma Feixiang , Cao Guiping , Lu Lu , Zhu Yingling , Li Wanlang , Chen Li 
Published: February 2024

ObjectiveTo explore the efficacy of electroacupuncture in treating post-stroke depression (PSD) by modulating the inflammatory response pathway.MethodsOne hundred and fifty participants with mild or moderate PSD were randomly divided into 75 cases each in the electroacupuncture group (EA group) and escitalopram group (ESC group). In the EA group, 30 sessions of electroacupuncture were performed on the Baihui (GV 20), Yintang (GV 29), and the ipsilateral Taichong (LR 3) and Hegu (LI 4), simultaneous oral placebo for 40 days. The ESC group received oral escitalopram oxalate tablets 10mg to 20mg for 40 days, plus 30 sessions of sham electroacupuncture. The effectiveness of the treatment was evaluated by the Hamilton Depression Scale (HAMD-17), Self-Depression Scale (SDS), Modified Barthel Index Score (MBI), and the serum levels of IL-1β, IL-6, IL-10, TNF-α, and INF-γ.ResultsThere was no statistically significant difference in the baseline data, HAMD-17, SDS, MBI scores, and serum IL-1β, IL-6, IL-10, TNF-α, and INF-γ levels between the two groups of participants before the intervention (P &gt;0.05). After treatment, HAMD-17 and SDS scores continued to decrease and MBI scores continued to increase in both groups. The differences were statistically significant at the 6th week and baseline, the 10th week and baseline, and the 10th week and the 6th week (all P &lt;0.001). The differences in HAMD-17, SDS, and MBI scores between the two groups at the 6th week were not statistically significant (P=0.110, 0.115, 0.516, respectively); HAMD-17 scores and SDS scores in the EA group were lower than those in the ESC group at the 10th week, and the differences were statistically significant (P=0.002,0.026, respectively). In the 6th week, the serum levels of pro-inflammatory factors such as IL-1β, IL-6, TNF-α, and INF-γ were significantly lower in both groups compared with the baseline, while the level of anti-inflammatory factor IL-10 was significantly higher. The difference between the pre-and post-intervention intra-group comparisons was statistically significant (P &lt;0.001), and the difference between the inter-group comparisons was not statistically significant (P &gt;0.05). No serious adverse events occurred throughout the trial. Both therapies could safely and effectively improve HAMD-17, SDS, and MBI scores and modulate neuroinflammatory responses in PSD participants. After the treatment was stopped, some parameters were better in the EA group than the ESC group in a short time.ConclusionElectroacupuncture is an effective, alternative to escitalopram for the treatment of mild-to-moderate PSD.Clinical trial registrationChinese Clinical Trial Registry (ChiCTR2300072576).
Factors affecting advance directives completion among older adults in Korea
Authors: Choi Seunghye , Ko Hana 
Published: February 2024

ObjectiveAdvance directives (ADs) provide an opportunity for patients to enhance the quality of their end-of-life care and prepare for a dignified death by deciding treatment plans. The purpose of this study was to explore the multiple factors that influence the advance directives completion among older adults in South Korea.MethodsThis was a secondary analysis of a cross-sectional study of 9,920 older adults. The differences in ADs based on subjects’ sociodemographic characteristics, health-related characteristics, and attitude toward death were tested using the chi-squared and t-test. A multinomial logistic regression model was used to identify the influencing factor of ADs.ResultsThe number of chronic diseases, number of prescribed medications, depression, insomnia, suicide intention, and hearing, vision, or chewing discomfort were higher in the ADs group compared to the non-ADs group. The influencing factors of the signing of ADs included men sex, higher education level, exercise, death preparation education, lower awareness of dying-well, and experience of fracture.ConclusionInformation dissemination regarding ADs should be promoted and relevant authorities should consider multiple options to improve the physical and psychological health of older adults, as well as their attitude toward death to increase the ADs completion rate.
Valuing the contribution of sport volunteering to subjective wellbeing: evidence from eight European countries
Authors: Kokolakakis Themistocles , Schoemaker Jelle , Lera-Lopez Fernando , de Boer Willem , Čingienė Vilma , Papić Alma , Ahlert Gerd 
Published: February 2024

IntroductionVolunteering is a prominent and integral aspect of the activities undertaken by sports clubs in Europe. However, even with its growing importance, quantifying the monetary worth of this nonmarket activity, in terms of wellbeing, can present certain difficulties. Traditional approaches to valuing volunteering (i.e., replacement and opportunity cost approaches) do not fully capture the value of volunteering to individuals, as they do not consider the intangible benefits that individuals may derive from their participation.MethodsThis research provides added value to the monetisation of volunteering in sport by applying the wellbeing valuation approach (WVA) for the first time to a cross-sectional data in eight European countries. A double instrumental variable approach was developed to correct for unobservable variables that may influence the pairs: income and subjective wellbeing (SWB), and volunteering and SWB. This allows to estimate the causal impact of volunteering and income on SWB more accurately and assign a reasonable monetary value to this non-market activity.ResultsThe results, based on a sample size of 1,091, show an income compensation for a volunteer, devoting on average 8.7 hours during a four-week period of €16 to €50 per hour, equivalent to between € 1,700 and € 5,200 per year, depending on the nationality of the volunteer.DiscussionWith these estimations insights into the value of volunteering in sports are provided, contributing to a better understanding of how this activity can be valued and supported. By recognising and accurately valuing the contributions of volunteers, sport organizations and policymakers can develop more effective strategies for promoting and supporting volunteering in sports.
An evaluation of the physiological uptake range of 18F-fluoro-2-deoxy-D-glucose in normal ovaries of seven dogs using positron emission tomography/computed tomography
Authors: Choi Jinyoung , Chae Yeon , Kang Byeong-Teck , Lee Sungin 
Published: February 2024

IntroductionThis study evaluated the physiological uptake range of 18F-fluoro-2-deoxy-D-glucose (18F-FDG) in the normal ovaries of seven dogs using positron emission tomography/computed tomography (PET/CT).Materials and methodsThe dogs were subjected to general anesthesia and were positioned in ventral recumbency for PET/CT scans. The dosage of 18F-FDG ranged from 0.14 to 0.17 mCi/kg and was administered intravenously followed by 0.9% NaCl flushing; PET/CT images of each dog were obtained precisely 60 min after the injection of 18F-FDG. The regions of interest were drawn manually, and standardized uptake values (SUV) were calculated to evaluate the 18F-FDG uptake in each ovary. The maximum and mean SUVs (SUV max and SUV mean) for all the ovaries of the dogs were then computed.ResultsThe range of SUV max and SUV mean of the normal ovaries of the dogs were 1.28–1.62 and 1.07–1.31 (mean ± standard deviation), respectively.ConclusionThis is the first study to investigate the normal 18F-FDG uptake baseline data of normal canine ovaries using PET/CT scans. These data will help clinicians in identifying malignant tumors before anatomical changes in the ovary through PET/CT scans.
Metahemoglobinemia em paciente com Lúpus Eritematoso Sistêmico, induzida por hanseníase
Authors: Dos Santos Railson Rodrigues 
Published: February 2024

Objetivo: Relatar o caso de uma paciente portadora de Lúpus Eritematoso Sistêmico que adquiriu hanseníase e evoluiu com Metahemoglobinemia. Detalhamento do caso: Paciente do sexo feminino, 26 anos de idade, portadora de Lúpus Eritematoso Sistêmico, adquiriu hanseníase, fazendo uso de esquema padrão medicações como Rifampicina, Clofazimina e Dapsona. Após 3 meses de tratamento, a paciente evoluiu com febre vespertina, tosse, astenia e dispneia por cerca de 3 semanas até a internação hospitalar, durante a internação várias hipóteses diagnósticas foram levantadas como tuberculose, COVID-19, entre outras. Mas, somente após reavaliações do caso e das gasometrias arteriais, foi constatado a presença de Metahemoglobinemia associada ao uso de Dapsona, com a suspensão dessa droga, houve melhora clínica da paciente. Considerações Finais: A coexistência de hanseníase em paciente Lúpus Eritematoso Sistêmico que evolui com Metahemoglobinemia é um achado raro e pode representar um dilema diagnóstico, tendo em vista as várias possibilidades de diagnóstico para dispneia. Dessa forma, é preciso uma constante avaliação do caso clínico, exames e raciocínio, a fim de elucidar o caso.
Ozonioterapia e seus efeitos no tratamento da esclerose múltipla: uma revisão sistemática
Authors: Rodrigues Lívia Layanne Lopes Fernandes , Aragão Laura Mourão , Bezerra Eduarda Feitosa , Alexandre Daniella Benício Ribeiro , Saraiva Bárbara Carlos , De Sousa Milena Nunes Alves 
Published: February 2024

Introdução: A esclerose múltipla (EM) é uma doença autoimune e inflamatória que cursa com a alterações no sistema nervoso central, causando redução da atividade cerebral e das funções motoras. Por sua vez, a ozonioterapia configura-se como uma intervenção alternativa, capaz de promover melhora no funcionamento cerebral. Essa proposta de tratamento, ainda pouco explorada, vem sendo estudada por sua possível resposta terapêutica em pacientes com EM. Objetivo: Avaliar, em pacientes com esclerose múltipla, se a ozonioterapia tem efeitos positivos na redução dos danos. Método: É uma Revisão Sistemática de Intervenção. Para busca dos estudos foram utilizados os descritores em inglês e interligados pelo operador booleano - “Multiple Sclerosis” AND “Ozone therapy” - os quais, posteriormente, foram pesquisados nas seguintes bases de dados - U.S. National Institutes of Health’s National Library of Medicine, Web of Science, The Search Portal for Life Sciences, ProQuest, ScienceDirect, EBSCO Host, Medline Plus e Biblioteca Virtual em Saúde. Como critérios de elegibilidade, foram selecionados Ensaios Clínicos Randomizados, sem restrição de idioma e tempo, excluindo-se os duplicados, resultando em uma amostra final de seis artigos. Resultados: Verificou-se, como principais efeitos da ozonioterapia, a redução da inflamação e do estresse oxidativo crônico, além do aumento do metabolismo cerebral, demonstrando que a ozonioterapia é uma alternativa terapêutica benéfica nos quadros de EM. Além disso, a auto-hemoterapia destacou-se como melhor terapêutica, seguida pela insuflação de ozônio via retal. Conclusão: Analisou-se que a ozonioterapia pode ser uma alternativa eficaz para o tratamento da esclerose múltipla em detrimento do uso de doses farmacológicas e, por conseguinte, propiciou a redução da toxicidade e das reações adversas nos indivíduos. Ressalta-se, no entanto, que tal temática ainda é pouco discutida em meio científico e, por isso, é preciso que mais pesquisas sejam feitas para ratificar cada vez mais o uso dessa terapia em pacientes com EM.
Childhood vulvar lichen sclerosus: the consequences of diagnostic delay
Authors: Furtado Yara Lucia , Gieler Caroline Rodrigues Cambeiro , Silveira Filomena Aste , Damian Amanda , Almeida Gutemberg 
Published: February 2024

Objectives: This series of 12 cases intends to raise awareness to Lichen Sclerosus, by illustrating its main clinical characteristics and complications, and by highlighting the importance of its early diagnosis. Methods: All of the patients discussed were attended at the Institute of Gynecology of the Federal University of Rio de Janeiro (UFRJ, Rio de Janeiro, Brazil), after being referred from primary care family medicine facilities. Results: We present 12 cases. The mean ages at the beginning of symptoms and clinical diagnosis were 6 and 9.25 years, respectively. The mean delay for diagnosis was 3.2 years. Vulvar pruritus and hypochromic macules were the most common initial symptoms. Complications included the fusion of the labia minora and clitoris entrapment. Cases with an earlier diagnosis had better responses to treatment with ultra-potent corticosteroids such as Clobetasol propionate ointment 0.05%. Conclusion: Diagnostic delay of LS leads to its progression and long-term physical-psychological consequences. It must be remembered as a differential diagnosis of vulvar pruritus in childhood.
Análise da relação entre a depressão pós parto e a saúde mental da mulher
Authors: Bianchi Caroline Scalabrini , Marassi Fernanda , Felzener Manoel Carlos Melillo 
Published: February 2024

A depressão pós-parto (DPP) trata-se de uma condição que afeta 10% a 15% das mulheres. Esta condição tem seu início em algum momento durante o primeiro ano do pós-parto, havendo maior incidência entre a quarta e oitava semana após o parto. A DPP se manifesta através de diversos sintomas, como irritabilidade, choro frequente, sentimentos de desamparo, desmotivação, desinteresse sexual, transtornos alimentares e do sono, ansiedade, dentre outros. O objetivo deste artigo é apresentar uma revisão bibliográfica acerca da depressão pós-parto. São abordados aspectos conceituais, fatores de risco associados a sua ocorrência e algumas repercussões da depressão pós-parto na relação materno-infantil. O conhecimento destes aspectos reveste-se de grande importância considerando as consequências prejudiciais às mães bem como ao desenvolvimento cognitivo, social e emocional de suas crianças.
Perfil epidemiológico do diagnóstico de neoplasias malignas de brônquios e pulmão no sudeste brasileiro no período de 2018 a 2022
Authors: Aragão Letícia Cavalcante da Costa , Calumby Thales Andrade , Mendes Samilly Hellen Ferreira , Ribeiro Gabriel Henrique Leite , Barros Alan de Paula Ferreira , Cartaxo Higor Braga 
Published: February 2024

Introdução: A neoplasia maligna dos brônquios e pulmões representa um desafio significativo para a saúde no Brasil, especialmente na região Sudeste, onde a incidência é notavelmente alta. Objetivo: Analisar a incidência de neoplasias malignas dos brônquios e pulmões na região Sudeste brasileira, durante o período de 2018 a 2022. Metodologia: Trata-se de um estudo epidemiológico, do tipo ecológico, descritivo, com a abordagem de análise quantitativa. Foi realizada a coleta de dados do Painel de Monitoramento de Tratamento Oncológico (Painel-oncologia) do Departamento de Informática do Sistema Único de Saúde (DATASUS). Considerou-se as variáveis: faixa etária, sexo, modalidades de tratamento e estadiamento. Resultado: A investigação examinou um total de 27.419 casos de diagnósticos de câncer brônquico e pulmonar na região Sudeste entre os anos de 2018 e 2022, exibindo um aumento notável de 24,63% no total de casos durante esse período especificado. O ano de 2022 marcou a maior contagem de diagnósticos. Entre os estados da região Sudeste, São Paulo exibiu o número mais substancial de casos, seguido por Minas Gerais e Rio de Janeiro. Em relação à idade, a câncer foi mais incidente entre indivíduos de 60 a 69 anos. A maior parte dos casos foi diagnosticado em fases tardia (II e IV) e a forma de tratamento mais comum foi a quimioterapia, seguida por intervenção cirúrgica. Conclusão: O estudo destaca as implicações significativas do câncer brônquico e pulmonar para a saúde da população no Brasil, especialmente na região Sudeste, onde a doença é mais incidente. A alta taxa de mortalidade associada a esses cânceres enfatiza a urgência de abordar o problema para melhorar o bem-estar dos indivíduos afetados.
Carga de trabalho em enfermagem de pacientes adultos internados no setor de infectologia de um hospital de alta complexidade
Authors: De Oliveira Geovanna Maria , Cardoso Juliana Fagundes Silva , Lima Daiane de Mendonça , De Sousa Roseane Aparecida Gonçalves , Sanches Mary Angela de Meneses , De Paula Júnior Newton Ferreira , Braga Iolanda Alves , Calili Thiago Vinicius Alves , Gomes Fabiola Alves , Mendes-Rodrigues Clesnan 
Published: February 2024

A carga de trabalho da equipe de enfermagem, a partir dos cuidados de pacientes adultos internados com doenças infecciosas é influenciada por fatores como gravidade da doença, comorbidades e complicações. Avaliar a carga de trabalho é fundamental para determinar o número de profissionais necessários para prestar assistência de qualidade. O objetivo do estudo foi avaliar a carga de trabalho da Enfermagem em setor de infectologia de um hospital de alta complexidade e de ensino da cidade de Uberlândia – MG, Brasil, entre os anos de 2013 e 2022. A análise de agrupamento formou três blocos de especialidades: 1: Reumatologia, Neurologia, Clínica Médica e Infectologia; 2: Cirurgia Geral e Traumatologia e 3: Ortopedia; Gastroenterologia, Ginecologia e Obstetrícia e Cardiologia. Também foi possível verificar que, o tipo de cuidado predominante foi o de cuidado intermediário, seguido do cuidado mínimo e do cuidado de alta dependência; comuns nas áreas de Gastroenterologia, Cardiologia e Cirurgia Geral. Os pacientes da Infectologia apresentaram cuidado intermediário como o mais prevalente. Dessa forma, observou-se que a carga de trabalho da equipe de enfermagem neste setor é variável no tempo, e é dependente da especialidade médica.