bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural basis for VLDLR recognition by eastern equine encephalitis virus
Authors: Yang Pan , Li Wanyu , Fan Xiaoyi , Pan Junhua , Mann Colin J. , Varnum Haley , Clark Lars E. , Clark Sarah A. , Coscia Adrian , Smith Katherine Nabel , Brusic Vesna , Abraham Jonathan 
Published: November 2023

SummaryAlphaviruses are arthropod-borne enveloped RNA viruses that include several important human pathogens with outbreak potential. Among them, eastern equine encephalitis virus (EEEV) is the most virulent, and many survivors develop neurological sequelae, including paralysis and intellectual disability. The spike proteins of alphaviruses comprise trimers of heterodimers of their envelope glycoproteins E2 and E1 that mediate binding to cellular receptors and fusion of virus and host cell membranes during entry. We recently identified very-low density lipoprotein receptor (VLDLR) and apolipoprotein E receptor 2 (ApoER2), two closely related proteins that are expressed in the brain, as cellular receptors for EEEV and a distantly related alphavirus, Semliki forest virus (SFV)1. The EEEV and SFV spike glycoproteins have low sequence homology, and how they have evolved to bind the same cellular receptors is unknown. Here, we used single-particle cryo-electron microscopy (cryo-EM) to determine structures of the EEEV and SFV spike glycoproteins bound to the VLDLR ligand-binding domain. The structures reveal that EEEV and SFV use distinct surfaces to bind VLDLR; EEEV uses a cluster of basic residues on the E2 subunit of its spike glycoprotein, while SFV uses two basic residues at a remote site on its E1 glycoprotein. Our studies reveal that different alphaviruses interact with the same cellular receptor through divergent binding modes. They further suggest that the ability of LDLR-related proteins to interact with viral spike proteins through very small footprints with flexible binding modes results in a low evolutionary barrier to the acquisition of LDLR-related proteins as cellular receptors for diverse sets of viruses.
Development of LRRK2 designed ankyrin-repeat proteins
Authors: Dederer Verena , Murillo Marta Sanz , Karasmanis Eva P , Hatch Kathryn S , Chatterjee Deep , Preuss Franziska , Abdul Azeez Kamal R , Nguyen Landon Vu , Galicia Christian , Dreier Birgit , Plückthun Andreas , Versees Wim , Mathea Sebastian , Leschziner Andres E , Reck-Peterson Samara L , Knapp Stefan 
Published: November 2023

AbstractLeucine rich repeat kinase 2 (LRRK2) is a large multidomain protein containing two catalytic domains, a kinase and a GTPase, as well protein interactions domains, including a WD40 domain. The association of increased LRRK2 kinase activity with both the familial and sporadic forms of Parkinson’s disease (PD) has led to intense interest in determining its cellular function. However, small molecule probes that can bind to LRRK2 and report on or affect its activity are needed. Here, we identified a series of high-affinity LRRK2-binding designed ankyrin-repeat proteins (DARPins). One of these DARPins (E11) bound to the LRRK2 WD40 domain with high affinity. LRRK2 bound to DARPin E11 showed improved behavior on cryo-EM grids, resulting in higher resolution LRRK2 structures. DARPin E11 did not affect the catalytic activity of a truncated form of LRRK2 in vitro but decreased the phosphorylation of Rab8A, a LRRK2 substrate, in cells. We also found that DARPin E11 disrupts the formation of microtubule-associated LRRK2 filaments in cells, which are known to require WD40-based dimerization. Thus, DARPin E11 is a new tool to explore the function and dysfunction of LRRK2 and guide the development of LRRK2 kinase inhibitors that target the WD40 domain instead of the kinase.
Meaningful outcomes of specialist leisure activities for children with complex disabilities: the views of parents, professionals and young people
Authors: Collins Bethan , McGrath Nicole , Astill Fiona , Hurt Lisa , Maguire Sabine , Kemp Alison 
Published: November 2023

AbstractLeisure activities during childhood are vital to quality of life and wellbeing, however parents report poor quality of life and infrequent leisure participation for children with complex disabilities. Sparkle, a charity in South Wales, delivers specialist leisure activities aimed at providing children with disabilities with access to the same opportunities as any other child. We explored the impact of this provision on psychosocial domains of quality of life for children with complex disabilities.Multi-source qualitative case studies including interviews and observations were conducted with: four children/young people - aged 8-15 years with diagnoses including autism, Down’s syndrome and cerebral palsy - accessing Sparkle’s leisure activities; their parents/carers and leisure staff supporting them. Data were analysed using coding reliability thematic analysis.Three themes were generated: self-development, friendship and social interaction, and self and family wellbeing. An overarching theme of the need for a specialist provision enabled the other themes linked to positive outcomes for the children.We concluded that a specialist provision contributes to positive psychosocial outcomes linked to leisure participation for children with complex disabilities. Limitations, future research and implications for policy and practice are discussed.
How optimal is Ghana's single-digit inflation targeting? An assessment of monetary policy effectiveness in Ghana
Authors: Amoatey Richard , Ayisi Richard K. , Osei-Assibey Eric 
Published: November 2023

PurposeThe purpose of this study is twofold. First, to estimate an optimal inflation rate for Ghana and second, to investigate factors that account for the differences between observed and target inflation.Design/methodology/approachThe paper explored the questions within two econometric frameworks, the Autoregressive Distributed Lag (ARDL) and Threshold Regression Models using data spanning the period 1965–2019.FindingsThe study estimated a range of 5–7% optimal inflation for Ghana. While this confirms the single-digit inflation targeting by the Bank of Ghana, the range is lower than the central bank's band of 6–10%. The combined behaviours of the central bank, banks and external outlook influence inflation target misses.Practical implicationsThe study urges the central bank to continue pursuing its single-digit inflation targeting. However, it implies that there is still room for the Bank to further lower the current inflation band to achieve an optimal outcome on growth and welfare. Again, the Bank should commit to increased transparency and accountability to enhance its credibility in attaining the targeted inflation.Originality/valueThe study is one of the first attempts in Africa in Ghana to estimate an optimal inflation target and investigate the underlying factors for deviation from the targets.
The influence of brand personality on brand love, perceived quality and purchase intention: a study of e-retailing sites
Authors: Kukreti Rajat , Yadav Mayank 
Published: November 2023

PurposeThis study aims to understand how brand personality affects purchase intention through brand love and perceived quality in e-commerce.Design/methodology/approachThree hundred forty-eight users of e-commerce sites in New Delhi, India, were surveyed for the study. The data set was examined using confirmatory factor analysis, and the research hypotheses were assessed using structural equation modeling.FindingsTwo important conclusions emerged from the study. First, brand love and perceived quality have been considerably and favorably influenced by all six dimensions of brand personality of e-commerce brands. Second, the purchase intention toward the e-commerce sites is significantly and positively impacted by brand love and perceived quality.Practical implicationsThis study by exploring various dimensions of brand personality, will assist e-commerce executives in increasing purchase intention toward the e-retailing sites.Originality/valueThis research is supposed to be the foremost to look at how brand personality, through brand love and perceived quality affects purchase intention toward e-commerce websites. The attachment theory is used in this study as a theoretical foundation for linking e-commerce brand personality to customers’ purchase intentions via brand love and perceived quality.
Value creation in the metaverse age: a thematic analysis of press releases
Authors: Ferrigno Giulio , Di Paola Nadia , Oguntegbe Kunle Francis , Kraus Sascha 
Published: November 2023

PurposeSince Zuckerberg's announcement to change Facebook's name to Meta Platforms Inc. on October 28, 2021, the concept of the metaverse has gained unprecedented popularity in the business world. Tech giants, SMEs and start-ups across various sectors are making substantial investments in metaverse-related technologies. Despite this, scholarly research in entrepreneurship and strategic management regarding the metaverse remains limited. This paper, grounded in value creation theory, aims to analyze how value is generated in the metaverse era.Design/methodology/approachThis paper conducts a thematic analysis of 895 press releases published by LexisNexis between October 28, 2021, and October 28, 2022. The analysis identifies the primary emerging themes related to value creation in the metaverse age.FindingsThe thematic analysis reveals four significant emerging themes concerning value creation in the metaverse age: (1) factors enabling value creation, (2) digital resources contributing to value creation, (3) motives driving value creation and (4) practices of value creation.Originality/valueThis paper represents the inaugural attempt to analyze the metaverse through a value creation lens. Given the substantial investments and growing academic interest in the metaverse, understanding value creation in this context is a pressing concern. Additionally, this study provides valuable insights and suggests critical questions for future research on the metaverse.
Empirical nexus of hospital brand gestalt, patient satisfaction and revisit intention
Authors: Mandagi Deske W. , Rampen Derby Chriestofle , Soewignyo Tonny Irianto , Walean Ronny H. 
Published: November 2023

Purpose
The purpose of this investigation is to scrutinize the unexplored realm concerning the interplay of hospital brand gestalt on patient satisfaction and revisit intentions.

Design/methodology/approach
A self-administered online survey was conducted with 227 patients who had stayed at and received health-care services from a private hospital in the city of Manado, Indonesia, within the past 12 months. The quantitative data were subsequently analyzed using a structural equation model with the assistance of Smart PLS statistical software.

Findings
The results suggest that the hospital brand gestalt significantly and positively influences patient satisfaction, which, in turn, leads to patients’ intentions to revisit. Furthermore, patient satisfaction serves as a significant mediator in the relationship between brand gestalt and revisit intentions.

Research limitations/implications
This study enhances the comprehension of brand gestalt’s influence on customer attitudes and behaviors within the health-care context, contributing to the expanding body of literature concerning holistic brand perception. For health-care providers, the study underscores the significance of creating a uniform and distinctive brand experience to boost patient satisfaction and cultivate loyalty. In summary, this study paves the way for strategic branding initiatives in health care, ultimately enhancing patient experiences and organizational outcomes.

Practical implications
For health-care providers, this study emphasizes the importance of crafting a consistent and differentiated brand experience to enhance patient satisfaction and foster loyalty. Overall, this study opens avenues for strategic branding efforts in health care, ultimately improving patient experiences and organizational outcomes.

Originality/value
While there is a growing interest in the role of brand gestalt in marketing research, there is still a need for more empirical research to explore the link between brand gestalt, customer satisfaction and revisit intention. Surprisingly, to the best of the authors’ knowledge, no previous studies have investigated the role of brand gestalt in the context of health care.
Millennial perceptions of private label and national brand apparel
Authors: Sorensen Katelyn , Johnson Jorgensen Jennifer 
Published: November 2023

PurposeThis paper aims to use Q methodology to investigate Millennial perceptions toward private label or national brand apparel.Design/methodology/approachQ methodology was chosen to identify factors, which correspond to patterns of perceptions prevalent among Millennials. Participants were supplied with 14 statements that they sorted into two Q sorts – One representing perceptions of private label and the other representing perceptions of national brands. The Q sorts were completed through Qualtrics and participants answered open-ended questions on the placement of each statement within each Q sort.FindingsTwo factors emerged on private labels, highlighting patterns in price consciousness and uniqueness (acknowledged as patterns surrounding the desire for particular apparel characteristics). Three factors arose for national brand apparel, emphasizing the need for national brands to provide consumers with product security, quality and uniqueness (as identified through the unpreferred qualities national brands typically exhibit).Originality/valueThis study illustrates the various viewpoints retailers must consider when marketing apparel to a specific target demographic. In addition, a single perception (uniqueness) was found to connect motivations, which led to the development of a model for future inquiry.Research limitations/implicationsDespite complete Q sorts and qualitative statements, participants' unfamiliarity with Q methodology and the sorting action of statements could be considered a limitation. The use of MTurk is also considered a limitation owing to the anonymity and possible deception of the workforce.Practical implicationsPrivate label brand personality growth has many retailers expanding their brand portfolios. Based on the findings of this study, specific opportunities are highlighted for the expansion and marketing of private labels and brand labels based on specific perceptions of a broad Millennial cohort.
When commuting becomes a pain: a daily diary study of the effect of commuting stressors on abusive supervision
Authors: Chen Yushuai , Ashkanasy Neal M. , Liu Xin , Wu Lijun , Yang An 
Published: November 2023

PurposeStudies of the antecedents of daily abusive supervision have mainly focused on work stressors and family stressors, ignoring the potential importance of commuting stressors that are encountered enroute to work. Based in affective events theory, the authors propose a daily, within-person model to examine how the commuting stressors faced by supervisors affect their propensity to engage in abusive supervision behavior and the mechanisms underlying this effect.Design/methodology/approachUsing experience-sampling methodology, the authors collected data from 49 supervisors in China who responded to two daily surveys for 10 working days.FindingsThe authors found that daily morning commuting anger mediates the link between daily morning commuting stressors and subsequent abusive supervision. The authors also found that trait-displaced aggression moderates this relationship, such that the mediating effect occurs only when supervisors' trait-displaced aggression is high rather than low.Originality/valueThis study enriches the antecedents of daily abusive supervision and extends the commuting literature to the leadership context.
Test anxiety's influence on attitudes, confidence and efficacy in STEM courses: a pilot study
Authors: Hyseni Duraku Zamira , Hoxha Linda , Konjufca Jon , Blakaj Artë , Bytyqi Blerinë , Mjekiqi Erona , Bajgora Shkurtë 
Published: November 2023

PurposeThis pilot study aims to examine the prevalence of test anxiety and its interplay with attitudes, confidence, efficacy, academic performance and socio-demographic factors within the domain of science, technology, engineering and mathematics (STEM) courses.Design/methodology/approachThe authors employed a quantitative, cross-sectional design with 549 sixth-grade students from public lower secondary schools in Prishtina, Kosovo, using the Student Attitudes Toward STEM Survey (S-STEM) for middle/high schools and the test anxiety questionnaire.FindingsOver 70% of Kosovo's sixth-grade students reported moderate to severe test anxiety. The age of students was found to be inversely related to academic performance in STEM. The father's employment was associated with favorable STEM attitudes, confidence, efficacy and academic performance. Having a personal study environment was connected with favorable STEM attitudes, confidence and efficacy in STEM, whereas access to technology was associated with positive academic performance. Test anxiety, academic performance and personal study space predicted students' attitudes, confidence and efficacy in STEM and 21st-century learning.Practical implicationsEducational institutions should prioritize student well-being. By addressing test anxiety, these institutions can create supportive learning environments that improve attitudes, confidence and efficacy in STEM fields. These efforts are crucial for STEM career development and student success in the 21st-century workforce.Originality/valueThe current study findings contribute to a deeper understanding of the factors influencing STEM student engagement and performance, highlighting the importance of addressing test anxiety for positive learning outcomes while emphasizing the need to consider socio-economic and contextual factors in education.
Do contingent liabilities affect dividend decisions?
Authors: Chaklader Barnali , Mundi Hardeep Singh 
Published: November 2023

PurposeThe paper examines contingent liabilities' effect on the firm's dividend decisions.Design/methodology/approachFixed-effects regression and logit model results estimate the influence of contingent liabilities on firms' dividend decisions using a sample of 2,288 firm-year observations of S&P 500 firms from 2012 until 2022. Robustness checks and results from the 2SLS model further support the authors’ findings.FindingsThe results show that contingent liabilities negatively affect dividend payment decisions. This analysis further demonstrates that the stated effect of contingent liabilities on dividend decisions is more substantial for firms with financing deficits and those with above-industry-average corporate governance scores.Research limitations/implicationsThere needs to be more systematic conceptual reason for measuring uncertainty for firms and its influence on dividend decisions. Future research should use other measures of firm uncertainty to examine the relation of the firm's uncertainty with dividend decisions.Practical implicationsThe authors suggest that contingent liabilities create uncertainty for future cash flows, influence a firm's agency costs and provide credible signals on a firm's prospects to the market. The findings support existing literature that measurable firm-specific variables significantly influence a firm's dividend decisions. The results are robust for an alternative explanation.Originality/valueBy investigating the impact of the influence of contingent liabilities on dividends, the authors extend research on dividend decisions and attempt to provide insights into a firm's dividend decisions by incorporating an off-the-balance sheet item (contingent liabilities) as a significant predictor for dividend decisions.
Typical faults and safety analysis of brake controller for AC locomotive
Authors: Ren Yujie , Chi Hai 
Published: November 2023

PurposeThe brake controller is a key component of the locomotive brake system. It is essential to study its safety.Design/methodology/approachThis paper summarizes and analyzes typical faults of the brake controller, and proposes four categories of faults: position sensor faults, microswitch faults, mechanical faults and communication faults. Suggestions and methods for improving the safety of the brake controller are also presented.FindingsIn this paper, a self-judgment and self-learning dynamic calibration method is proposed, which integrates the linear error of the sensor and the manufacturing and assembly errors of the brake controller to solve the output drift. This paper also proposes a logic for diagnosing and handling microswitch faults. Suggestions are proposed for other faults of brake controller.Originality/valueThe methods proposed in this paper can greatly improve the usability of the brake controller and reduce the failure rate.
Low and high nucleic acid content bacteria play discrepant roles in response to various carbon supply modes
Authors: Zhang Hui , Hu Wei , Liu Ruidan , Bartlam Mark , Wang Yingying 
Published: November 2023

AbstractPlanktonic bacteria can be grouped into ‘high nucleic acid content (HNA) bacteria’ and ‘low nucleic acid content (LNA) bacteria.’ Nutrient input modes vary in environments, causing nutrient availability heterogeneity. We incubated them with equal amounts of total glucose added in a continuous/pulsed mode. The pulse‐treated LNA bacteria exhibited twice the cell abundance and four times the viability of the continuous‐treated LNA, while HNA did not show an adaptation to pulsed treatment. In structural equation modelling, LNA bacteria had higher path coefficients than HNA, between growth and carbon‐saving metabolic pathways, intracellular ATP and the inorganic energy storage polymer, polyphosphate, indicating their low‐cost growth, and flexible energy storage and utilisation. After incubation, the pulse‐treated LNA bacteria contained more proteins and polysaccharides (0.00064, 0.0012 ng cell−1) than the continuous‐treated LNA (0.00014, 0.00014 ng cell−1), conferring endurance and rapid response to pulses. Compared to LNA, HNA keystone taxa had stronger correlations with the primary glucose metabolism step, glycolysis, and occupied leading positions to explain the random forest model. They are essential to introduce glucose into the element cycling of the whole community under both treatments. Our work outlines a systematic bacterial response to carbon input.