bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between co-infection with Chlamydia trachomatis or Mycoplasma genitalium and cervical lesions in HPV-positive population in Hunan, China: a cross-sectional study
Authors: Jiang Mengjie , Ding Hui , He Ling , Xu Danning , Jiang Ping , Tang Haoneng , Wang Qian , Wang Xuemei , Tang Lingli 
Published: November 2023

Abstract
Objectives
The aim of this study was to determine the prevalence of Chlamydia trachomatis (CT) and Mycoplasma genitalium (MG) among HPV-positive women undergoing colposcopy at the Second Xiangya Hospital of Central South University, Hunan, China. Additionally, we aimed to assess the impact of C. trachomatis or M. genitalium co-infection with HPV on the severity of cervical lesions.

Methods
We collected HPV data, cervical cytology results, and demographic information from 439 women attending colposcopy. Cervical swabs were obtained for simultaneous amplification testing (SAT) of C. trachomatis and M. genitalium. Multivariate logistic regression analyses were performed to examine the association between sexually transmitted pathogens and cervical lesions.

Results
Among the participants, C. trachomatis was detected in 17 (3.87%) individuals, and M. genitalium in 16 (3.64%) individuals. There was no co-infection of C. trachomatis and M. genitalium. The highest prevalence of M. genitalium was observed in women aged 19–30 years (10.20%; 95% CI, 1.41-18.99%), with a subsequent decline in prevalence with increasing age (Ptrend = 0.014). The most common HPV subtype in our study was HPV52 (30.79%), followed by HPV16 (18.62%), HPV58 (16.95%), and HPV53 (10.02%). Infection with HPV16 (OR = 3.43, 95% CI, 2.13–5.53), HPV31 (OR = 3.70, 95% CI, 1.44–9.50), and HPV33 (OR = 3.71, 95% CI, 1.43–9.67) was associated with an increased severity of cervical lesions, while HPV53 infection was not likely to lead to advanced cervical lesions (OR = 0.45, 95% CI, 0.23–0.89). The leukocyte level in vaginal secretions (P = 0.042) and cervical cytology results (P < 0.001) showed associations with the degree of cervical lesions. However, there was no significant association between C. trachomatis or M. genitalium infection and the severity of cervical lesions, nor with their co-infection with HPV16.

Conclusions
There was no correlation between co-infection of Chlamydia trachomatis or Mycoplasma genitalium and the degree of cervical lesions in HPV-positive population in Hunan, China. Our findings emphasized the need to pay more attention to M. genitalium infection among young women. Increased levels of leukocytes in vaginal secretions may be linked to cervical lesions. HPV16, HPV31, and HPV33 in Hunan province, China, may exhibit higher cervical pathogenicity.
Imp is expressed in INPs and newborn neurons where it regulates neuropil targeting in the central complex
Authors: Munroe Jordan A. , Doe Chris Q. 
Published: November 2023

AbstractThe generation of neuronal diversity remains incompletely understood. In Drosophila, the central brain is populated by neural stem cells derived from progenitors called neuroblasts (NBs). There are two types of NBs, type 1 and 2. T1NBs have a relatively simple lineage, whereas T2NBs expand and diversify the neural population with the generation of intermediate neural progenitors (INPs), contributing many neurons to the adult central complex, a brain region essential for navigation. However, it is not fully understood how neural diversity is created in T2NB and INP lineages. Imp, an RNA-binding protein, is expressed in T2NBs in a high-to-low temporal gradient, while the RNA-binding protein Syncrip forms an opposing gradient. It remains unknown if Imp expression is carried into INPs; whether it forms a gradient similar to NBs; and whether INP expression of Imp is required for generating neuronal identity or morphology. Here, we show that Imp/Syp are both present in INPs, but not always in opposing gradients. We find that newborn INPs adopt their Imp/Syp levels from their parental T2NBs; that Imp and Syp are expressed in stage-specific high-to-low gradients in INPs. In addition, there is a late INP pulse of Imp. We find that neurons born from old INPs (E-PG and PF-R neurons) have altered morphology following both Imp knock-down and Imp overexpression. We conclude that Imp functions in INPs and newborn neurons to determine proper neuronal morphology and central complex neuropil organization.
The effect of TLR3 priming conditions on MSC immunosuppressive properties
Authors: Tolstova Tatiana , Dotsenko Ekaterina , Kozhin Peter , Novikova Svetlana , Zgoda Victor , Rusanov Alexander , Luzgina Nataliya 
Published: November 2023

Abstract
Background
Mesenchymal stromal cells (MSCs) have regenerative and immunomodulatory properties, making them suitable for cell therapy. Toll-like receptors (TLRs) in MSCs respond to viral load by secreting immunosuppressive or proinflammatory molecules. The expression of anti-inflammatory molecules in MSCs can be altered by the concentration and duration of exposure to the TLR3 ligand polyinosinic-polycytidylic acid (poly(I:C)). This study aimed to optimize the preconditioning of MSCs with poly(I:C) to increase immunosuppressive effects and to identify MSCs with activated TLR3 (prMSCs).

Methods
Flow cytometry and histochemical staining were used to analyze MSCs for immunophenotype and differentiation potential. MSCs were exposed to poly(I:C) at 1 and 10 μg/mL for 1, 3, and 24 h, followed by determination of the expression of IDO1, WARS1, PD-L1, TSG-6, and PTGES2 and PGE2 secretion. MSCs and prMSCs were cocultured with intact (J−) and activated (J+) Jurkat T cells. The proportion of proliferating and apoptotic J+ and J− cells, IL-10 secretion, and IL-2 production after cocultivation with MSCs and prMSCs were measured. Liquid chromatography–mass spectrometry and bioinformatics analysis identified proteins linked to TLR3 activation in MSCs.

Results
Poly(I:C) at 10 μg/mL during a 3-h incubation caused the highest expression of immunosuppression markers in MSCs. Activation of prMSCs caused a 18% decrease in proliferation and a one-third increase in apoptotic J+ cells compared to intact MSCs. Cocultures of prMSCs and Jurkat cells had increased IL-10 and decreased IL-2 in the conditioned medium. A proteomic study of MSCs and prMSCs identified 53 proteins with altered expression. Filtering the dataset with Gene Ontology and Reactome Pathway revealed that poly(I:C)-induced proteins activate the antiviral response. Protein‒protein interactions by String in prMSCs revealed that the antiviral response and IFN I signaling circuits were more active than in native MSCs. prMSCs expressed more cell adhesion proteins (ICAM-I and Galectin-3), PARP14, PSMB8, USP18, and GBP4, which may explain their anti-inflammatory effects on Jurkat cells.

Conclusions
TLR3 activation in MSCs is dependent on exposure time and poly(I:C) concentration. The maximum expression of immunosuppressive molecules was observed with 10 µg/mL poly(I:C) for 3-h preconditioning. This priming protocol for MSCs enhances the immunosuppressive effects of prMSCs on T cells.
Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment
Authors: Xie Zhenyu , Niu Liaoran , Zheng Gaozan , Du Kunli , Dai Songchen , Li Ruikai , Dan Hanjun , Duan Lili , Wu Hongze , Ren Guangming , Dou Xinyu , Feng Fan , Zhang Jian , Zheng Jianyong 
Published: November 2023

AbstractThe role of mast cells (MCs) in colorectal cancer (CRC) remains unclear, and a comprehensive single-cell study on CRC MCs has not been conducted. This study used a multi-omics approach, integrating single-cell sequencing, spatial transcriptomics, and bulk tissue sequencing data to investigate the heterogeneity and impact of MCs in CRC. Five MC signature genes (TPSAB1, TPSB2, CPA3, HPGDS, and MS4A2) were identified, and their average expression was used as a marker of MCs. The MC density was found to be lower in CRC compared to normal tissue, but MCs in CRC demonstrated distinct activation features. Activated MCs were defined by high expression of receptors and MC mediators, while resting MCs had low expression. Most genes, including the five MC signature genes, were expressed at higher levels in activated MCs. The MC signature was linked to a better prognosis in both CRC and pan-cancer patient cohorts. Elevated KITLG expression was observed in fibroblasts and endothelial cells in CRC samples compared to normal tissue, and co-localization of MCs with these cell types was revealed by spatial transcriptome analysis. In conclusion, this study finds decreased MC density in CRC compared to normal tissue, but highlights a shift in MC phenotype from CMA1high resting cells to activated TPSAB1high, CPA3high, and KIThigh cells. The elevated KITLG expression in the tumor microenvironment’s fibroblasts and endothelial cells may activate MCs through the KITLG-KIT axis, potentially suppressing tumor progression.
Routing protocols based on node selection for freely floating underwater wireless sensor networks: a survey
Authors: Alqahtani Ghida Jubran , Bouabdallah Fatma 
Published: November 2023

AbstractRecently, there has been an increasing interest in monitoring and exploring the underwater environment for scientific applications such as oceanographic data collection, marine surveillance, and pollution detection. Underwater acoustic sensor networks (UASN) have been proposed as the enabling technology to observe, map and explore the ocean. Due to the unique characteristics of underwater aquatic environment, which are low bandwidth, long propagation delays, and high energy consumption, the data forwarding process is very difficult. This paper presents a survey of the routing protocols for UASN. The addressed routing protocols are classified from a mobility point of view in freely floating underwater sensor networks. Indeed, managing the mobility of freely floating underwater sensors is one of the most critical constraints in the design of routing protocols. That is why we classify the routing protocols into “reliable data forwarding protocols” and “prediction-based data forwarding protocols.” In the first category, the proposed protocols mainly endure nodes’ mobility by continuously updating location information aiming at delivering the packets to the sink. In the second category, routing protocols try to rather master the nodes’ mobility by predicting the future nodes’ positions either based on a mobility model or on historical nodes’ positions using filtering techniques. We believe that our classification will help not only in deeply understanding the main characteristics of each protocol but also in investigating the evolution of research work evolution to provide energy-efficient data forwarding solutions for freely floating UASN.
Mobile service delivery in response to the opioid epidemic in Philadelphia
Authors: Stewart Rebecca E. , Christian Hanna P. , Cardamone Nicholas C. , Abrams Catherine , Drob Caroline , Mandell David S. , Metzger David , Lowenstein Margaret 
Published: November 2023

Abstract
Background
The harms of opioid use disorder (OUD) and HIV infection disproportionately impact marginalized populations, especially people experiencing homelessness and people who inject drugs (PWID). Mobile OUD service delivery models are emerging to increase access and reduce barriers to OUD care. While there is growing interest in these models, there is limited research about the services they provide, how they operate, and what barriers they face. We characterize the capacity, barriers, and sustainment of mobile OUD care services in a large city with a high incidence of OUD and HIV.

Methods
From May to August 2022, we conducted semi-structured interviews with leadership from all seven mobile OUD care units (MOCU) providing a medication for OUD or other substance use disorder services in Philadelphia. We surveyed leaders about their unit’s services, staffing, operating location, funding sources, and linkages to care. Leaders were asked to describe their clinical approach, treatment process, and the barriers and facilitators to their operations. Interview recordings were coded using rapid qualitative analysis.

Results
MOCUs are run by small, multidisciplinary teams, typically composed of a clinician, one or two case managers, and a peer recovery specialist or outreach worker. MOCUs provide a range of services, including medications for OUD, wound care, medical services, case management, and screening for infectious diseases. No units provide methadone, but all units provide naloxone, six write prescriptions for buprenorphine, and one unit dispenses buprenorphine. The most frequently reported barriers include practical challenges of working on a MOCU (e.g. lack of space, safety), lack of community support, and patients with substantial medical and psychosocial needs. Interviewees reported concerns about funding and specifically as it relates to providing their staff with adequate pay. The most frequently reported facilitators include positive relationships with the community, collaboration with other entities (e.g. local nonprofits, the police department, universities), and having non-clinical staff (e.g. outreach workers, peer recovery specialists) on the unit.

Conclusions
MOCUs provide life-saving services and engage marginalized individuals with OUD. These findings highlight the challenges and complexities of caring for PWID and demonstrate a need to strengthen collaborations between MOCU providers and the treatment system. Policymakers should consider programmatic funding for permanent mobile OUD care services.
Prognostic value of oxygen saturation index trajectory phenotypes on ICU mortality in mechanically ventilated patients: a multi-database retrospective cohort study
Authors: Shi Xiawei , Shi Yangyang , Fan Liming , Yang Jia , Chen Hao , Ni Kaiwen , Yang Junchao 
Published: November 2023

Abstract
Background
Heterogeneity among critically ill patients undergoing invasive mechanical ventilation (IMV) treatment could result in high mortality rates. Currently, there are no well-established indicators to help identify patients with a poor prognosis in advance, which limits physicians’ ability to provide personalized treatment. This study aimed to investigate the association of oxygen saturation index (OSI) trajectory phenotypes with intensive care unit (ICU) mortality and ventilation-free days (VFDs) from a dynamic and longitudinal perspective.

Methods
A group-based trajectory model was used to identify the OSI-trajectory phenotypes. Associations between the OSI-trajectory phenotypes and ICU mortality were analyzed using doubly robust analyses. Then, a predictive model was constructed to distinguish patients with poor prognosis phenotypes.

Results
Four OSI-trajectory phenotypes were identified in 3378 patients: low-level stable, ascending, descending, and high-level stable. Patients with the high-level stable phenotype had the highest mortality and fewest VFDs. The doubly robust estimation, after adjusting for unbalanced covariates in a model using the XGBoost method for generating propensity scores, revealed that both high-level stable and ascending phenotypes were associated with higher mortality rates (odds ratio [OR]: 1.422, 95% confidence interval [CI] 1.246–1.623; OR: 1.097, 95% CI 1.027–1.172, respectively), while the descending phenotype showed similar ICU mortality rates to the low-level stable phenotype (odds ratio [OR] 0.986, 95% confidence interval [CI] 0.940–1.035). The predictive model could help identify patients with ascending or high-level stable phenotypes at an early stage (area under the curve [AUC] in the training dataset: 0.851 [0.827–0.875]; AUC in the validation dataset: 0.743 [0.709–0.777]).

Conclusions
Dynamic OSI-trajectory phenotypes were closely related to the mortality of ICU patients requiring IMV treatment and might be a useful prognostic indicator in critically ill patients.
Near‐Infrared Spectroscopy measurements are reliable for studying patellar bone hemodynamics and affected by venous occlusion, but not by skin compression
Authors: Ophey Martin J. , Westerweel Anne , van Oort Maxime , van den Berg Robert , Kerkhoffs Gino M. M. J. , Tak Igor J. R. 
Published: November 2023

AbstractPurposeAccording to the homeostasis model, patellofemoral pain (PFP) results from disturbed homeostasis due to vascular insufficiency in the anterior knee. Near‐Infrared Spectroscopy (NIRS) measures relative changes in concentrations (in µmol/cm2) of (de‐)oxygenated hemoglobine (HHb and O2Hb). The aims were to: 1) investigate the characteristics of the NIRS signal derived from the patella during experiments affecting hemodynamics in healthy controls, and 2) determine the test–retest reliability of NIRS in positions clinically relevant for PFP patients.MethodsTwo experiments were conducted on 10 healthy controls and analysed using Student’s t‐test. Reliability (ICC2,1) was evaluated for two activities (‘Prolonged Sitting’ and ‘Stair Descent’) in five PFP patients and 15 healthy controls, performed twice within five days.ResultsThe NIRS signal (HHb and O2Hb) showed a statistically significant increase (p < .001 –. 002) on all optodes (30, 35, 40 mm) during ‘Venous Occlusion’ (M = 1.0 – 2.0), while it showed no statistically significant change (p = .075 –. 61) during ‘Skin Compression’ (M = ‐0.9 – 0.9) on the 30 and 35 mm optode. Reliability of NIRS (HHb and O2Hb) ranged from moderate to almost perfect (ICC2,1 = .47 –. 95) on the 30 mm optode for ‘Prolonged Sitting’, and from moderate to substantial (ICC2,1 = .50 –. 68) on the 35 mm optode for ‘Stair Descent’.ConclusionsPatella NIRS measurements are affected by venous occlusion, but not by skin compression, and are sufficiently reliable as research application to compare real‐time patellar bone hemodynamics. These insights may assist to improve effectiveness of evidence‐based treatment strategies for PFP.Trial registrationISRCTN Trial Registration under number: 90377123.
Pain as bad as you can imagine or extremely severe pain? A randomized controlled trial comparing two pain scale anchors
Authors: Tin Amy L. , Austria Mia , Ogbennaya Gabriel , Chimonas Susan , Andréll Paulin , Atkinson Thomas M. , Vickers Andrew J. , Carlsson Sigrid V. 
Published: November 2023

Abstract
Background
A common method of pain assessment is the numerical rating scale, where patients are asked to rate their pain on a scale from 0 to 10, where 0 is “no pain” and 10 is “pain as bad as you can imagine”. We hypothesize such language is suboptimal as it involves a test of a cognitive skill, imagination, in the assessment of symptom severity.

Methods
We used a large-scale online research registry, ResearchMatch, to conduct a randomized controlled trial to compare the distributions of pain scores of two different pain scale anchors. We recruited adults located in the United States who reported a chronic pain problem (> 3 months) and were currently in pain. Participants were randomized in a 1:1 ratio to receive pain assessment based on a modified Brief Pain Inventory (BPI), where the anchor for a score of 10 was either “extremely severe pain”, or the original BPI, with the anchor “pain as bad as you can imagine”. Participants in both groups also answered additional questions about pain, other symptomatology and creativity.

Results
Data were obtained from 405 participants for the modified and 424 for the original BPI. Distribution of responses to pain questions were similar between groups (all p-values ≥ 0.12). We did not see evidence that the relationship between pain score and the anchor text differed based on self-perceived creativity (all interaction p-values ≥ 0.2). However, in the key analysis, correlations between current pain assessments and known correlates (fatigue, anxiety, depression, current pain compared to a typical day, pain compared to other people) were stronger for “extreme” vs. “imaginable” anchor text (p = 0.005).

Conclusion
Pain rating scales should utilize the modified anchor text “extremely severe pain” instead of “pain as bad as you can imagine”. Further research should explore the effects of anchors for other symptoms.
Stigma perception and determinants among patients with type 2 diabetes mellitus in Iraq
Authors: Taher Taqi M. J. , Ahmed Hussein A. , Abutiheen Ali A. , Alfadhul Shaymaa A. , Ghazi Hasanain F. 
Published: November 2023

Abstract
Background and purpose
Diabetes mellitus (DM) is a leading cause of death worldwide. Stigma is a sign of social disgrace occurring within public relations, and it is linked with many health conditions including diabetes. Stigma could worsen the disease course, reduce treatment adherence, and affect the quality of life of diabetic patients. The objective of this study was to assess the magnitude of diabetic stigma among patients with type 2 DM.

Methods
In this analytic cross-sectional study, data collection was performed from June 1, 2022, until November 1, 2022, et al.-Najaf City, Iraq. A consecutive sample of 429 patients with type 2 DM was interviewed using the Arabic version of the type 2 Diabetes Stigma Assessment Scale (DSAS-2), which is a validated tool. The total diabetic stigma score, treated differently score, self-stigma score, and blame and judgment score were estimated.

Results
The mean age of the sample was 56.6 years, and males represented 61.8% of them. The total diabetic stigma score mean was 51.72. The question regarding people's judgment of food choices showed the highest rate (53%) among patients. Problematic stigma appeared in 24.71% of DM patients. Lower educational level, being divorced or widow, age above 50 years, being unemployed or housewife, and lower income showed significantly higher diabetic stigma scores.

Conclusion
One-quarter of type 2 DM patients showed problematic stigma. The mean diabetic stigma score was significantly higher among patients with lower education, divorced or widow status, older age, unemployment or housewife category, and low-income status.
Masseter vestibular-evoked myogenic potentials at different tone burst frequencies in healthy individuals
Authors: Thirusangu Vinayagar Pazhani , Sinha Sujeet Kumar 
Published: November 2023

Abstract
Background
Masseter vestibular-evoked myogenic potentials (mVEMP) are vestibular-dependent inhibitory reflex recorded using acoustic stimulus. mVEMP can assess important brainstem regions. mVEMP abnormalities have been reported in patients with multiple sclerosis, Parkinson’s disease, and idiopathic random eye movement disorder. The objective of the study was to record mVEMP using tone-burst stimulus at different frequencies in young healthy individuals.

Method
The study used normative study design. Thirty-two participants, aged 18–28 years with normal hearing and balance function, were recruited for the study. Simple random sampling was used for selection of participants.

Data collection and analysis
mVEMP was recorded for all the individuals at 500 Hz,750 Hz,1000 Hz, 2000 Hz, and 4000 Hz tone burst stimuli. The p11 and n21 peaks were identified. The latency of the p11 and n21 peaks and the rectified amplitude of the p11-n21 peak complex were measured for all the participants. Wilcoxson signed-rank test was used for statistical anlysis.

Results
The study found a 100% response rate and higher amplitude for 500 Hz and 750 Hz tone burst stimuli. However, Wilcoxson signed-rank test with Bonferroni correction showed no significant difference in amplitude (p = 0.92) between 500 and 750 Hz.

Conclusion
The study recommends 500 Hz/750 Hz tone burst stimuli as an optimal frequency for recording mVEMP in young, healthy individuals. However, seeing the frequency-tuning characteristics of mVEMP in other peripheral vestibular pathologies would be interesting.