bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibacterial mechanism of inosine against Alicyclobacillus acidoterrestris
Authors: Liu Xiaoxue , Wu Youzhi , Ran Junjian , Jiao Lingxia , Sun Linjun , Ye Fuzhou 
Published: January 2024

AbstractInosine could potentially become a novel antibacterial agent against Alicyclobacillus acidoterrestris as low doses of inosine can prevent its contamination. However, until now the antibacterial mechanism of inosine targeting A. acidoterrestris is still unknown. In this study, to unravel the mechanism of inosine against A. acidoterrestris puzzle, the effects of inosine on bacterial surface hydrophobicity, intracellular protein content, cell membrane damage extent, and permeability of the A. acidoterrestris were investigated. The results showed that inosine can effectively inhibit the growth and reproduction of A. acidoterrestris by destroying the integrity of cell membrane and increasing its permeability, causing the leakage of intracellular nutrients. Furthermore, the interaction networks of inosine target proteins were analyzed. The interaction networks further revealed that damage to bacterial cell membranes might be relevant to inosine's effect on bacterial DNA replication and cell energy metabolism through regulating nucleotide synthesis and metabolism and the activity of translation initiation factors. Finally, the antibacterial mechanism of inosine against A. acidoterrestris was proposed.
Characterising the metabolic rewiring of extremely slow growing Komagataella phaffii
Authors: Coltman Benjamin Luke , Rebnegger Corinna , Gasser Brigitte , Zanghellini Jürgen 
Published: January 2024

AbstractRetentostat cultivations have enabled investigations into substrate‐limited near‐zero growth for a number of microbes. Quantitative physiology at these near‐zero growth conditions has been widely discussed, yet characterisation of the fluxome is relatively under‐reported. We investigated the rewiring of metabolism in the transition of a recombinant protein‐producing strain of Komagataella phaffii to glucose‐limited near‐zero growth rates. We used cultivation data from a 200‐fold range of growth rates and comprehensive biomass composition data to integrate growth rate dependent biomass equations, generated using a number of different approaches, into a K. phaffii genome‐scale metabolic model. Here, we show that a non‐growth‐associated maintenance value of 0.65  and a growth‐associated maintenance value of 108  lead to accurate growth rate predictions. In line with its role as energy source, metabolism is rewired to increase the yield of ATP per glucose. This includes a reduction of flux through the pentose phosphate pathway, and a greater utilisation of glycolysis and the TCA cycle. Interestingly, we observed activity of an external, non‐proton translocating NADH dehydrogenase in addition to the malate–aspartate shuttle. Regardless of the method used for the generation of biomass equations, a similar, yet different, growth rate dependent rewiring was predicted. As expected, these differences between the different methods were clearer at higher growth rates, where the biomass equation provides a much greater constraint than at slower growth rates. When placed on an increasingly limited glucose diet, the metabolism of K. phaffii adapts, enabling it to continue to drive critical processes sustaining its high viability at near‐zero growth rates.
Transcriptional landscape of the pMP7017 megaplasmid and its impact on the Bifidobacterium breveUCC2003 transcriptome
Authors: Dineen Rebecca L. , Bottacini Francesca , O'Connell‐Motherway Mary , van Sinderen Douwe 
Published: January 2024

AbstractThe 190 kb megaplasmid pMP7017 of Bifidobacterium breve JCM7017 represents the first conjugative and largest plasmid characterised within this genus to date. In the current study, we adopted an integrated approach combining transcriptomics, whole genome comparative analysis and metagenomic data mining to understand the biology of pMP7017 and related megaplasmids, and to assess the impact of plasmid‐carriage on the host strain. The data generated revealed variations within basic features of promoter elements which correlate with a high level of transcription on the plasmid and highlight the transcriptional activity of genes encoding both offensive and defensive adaptations, including a Type IIL restriction‐modification system, an anti‐restriction system and four Type II toxin‐antitoxin systems. Furthermore, a highly transcribed tmRNA, which likely provides translational support to the host strain, was identified, making pMP7017 the first plasmid of the Bifidobacterium genus and the smallest plasmid known to express a tmRNA. Analyses of synteny and variability among pMP7017 and related plasmids indicate substantial diversity in gene organisation and accessory gene cargo highlighting diverse (co‐)evolution and potential host‐specific rearrangements and adaptations. Systematic analysis of the codon usage profile of transcriptionally active pMP7017‐encoded genes suggests that pMP7017 originated from (sub)species of Bifidobacterium longum. Furthermore, mining of metagenomic data suggests the presence of pMP7017‐homologues in ~10% of microbiome samples, mostly infants and/or mothers from various geographical locations. Comparative transcriptome analysis of the B. breve UCC2003 chromosome in the presence or absence of pMP7017 revealed differential expression of genes representing 8% of the total gene pool. Genes involved in genetic information processing were exclusively upregulated, while altered expression of genes involved in biofilm production and polysaccharide biosynthesis was also observed.
Age‐regulated cycling metabolites are relevant for behavior
Authors: Schwarz Jessica E. , Sengupta Arjun , Guevara Camilo , Barber Annika F. , Hsu Cynthia T. , Zhang Shirley L. , Weljie Aalim , Sehgal Amita 
Published: January 2024

AbstractCircadian cycles of sleep:wake and gene expression change with age in all organisms examined. Metabolism is also under robust circadian regulation, but little is known about how metabolic cycles change with age and whether these contribute to the regulation of behavioral cycles. To address this gap, we compared cycling of metabolites in young and old Drosophila and found major age‐related variations. A significant model separated the young metabolic profiles by circadian timepoint, but could not be defined for the old metabolic profiles due to the greater variation in this dataset. Of the 159 metabolites measured in fly heads, we found 17 that cycle by JTK analysis in young flies and 17 in aged. Only four metabolites overlapped in the two groups, suggesting that cycling metabolites are distinct in young and old animals. Among our top cyclers exclusive to young flies were components of the pentose phosphate pathway (PPP). As the PPP is important for buffering reactive oxygen species, and overexpression of glucose‐6‐phosphate dehydrogenase (G6PD), a key component of the PPP, was previously shown to extend lifespan in Drosophila, we asked if this manipulation also affects sleep:wake cycles. We found that overexpression in circadian clock neurons decreases sleep in association with an increase in cellular calcium and mitochondrial oxidation, suggesting that altering PPP activity affects neuronal activity. Our findings elucidate the importance of metabolic regulation in maintaining patterns of neural activity, and thereby sleep:wake cycles.
Sustainability in healthcare: patient and public perspectives
Authors: Knagg R. , Dorey J. , Evans R. , Hitchman J. 
Published: January 2024

SummaryThe sustainable healthcare agenda has become increasingly prominent in recent years. But what does this mean for patients? In this article, we draw on our personal views and experiences as patients, carers and patient advocates, and consider the effects that efforts to improve the sustainability of healthcare may have on care quality and patient experience. We also review the small amount of existing research and policy in this area, with particular focus on documents from the National Institute for Health and Care Excellence and the Health Foundation. Based on synthesising these resources with our own experiences, we make recommendations on how to: share information with patients about how they can contribute to healthcare sustainability; offer more sustainable alternatives without pressure; account for diverse patient views on the relevance of sustainable healthcare; provide information about the impact of healthcare on the environment; involve patients and the public in leading positive change; and avoid broadening health inequalities. There is a clear need for more research and engagement to help advance our understanding and weigh up the benefits to individual patients vs. the environmental impacts on the wider population.
The science of climate change and the effect of anaesthetic gas emissions
Authors: Slingo J. M. , Slingo M. E. 
Published: January 2024

SummaryThe dedication of the international anaesthetic community to reducing the environmental impact of healthcare is important and to be celebrated. When this is underpinned by robust science, it has the potential to make a real difference. However, volatile anaesthetic agents have been widely promoted in the medical literature as damaging to the climate, leading to a drive to remove them from clinical practice. This is based on notional ‘CO2‐equivalent’ values created using the simple emission metric known as the global warming potential. Here, we assert that when proper consideration is given to the science of climate change, volatile anaesthetic gas emissions cannot be simply equated to real carbon dioxide emissions, and that their climate impact is vanishingly small. This paper gives anaesthetists a framework to make informed choices founded on climate science and calls for attention to be refocused on the urgent need to reduce the real carbon dioxide emissions associated with healthcare.
Diagnostic adequacy and accuracy of surgeon‐performed ultrasound guided fine needle aspiration in lateral neck masses
Authors: Blom Megan , Magarey Matthew J. R. , Flatman Samuel 
Published: January 2024

AbstractBackgroundSurgeon‐performed ultrasound guided fine needle aspiration (SUS‐FNA) reduces the time to diagnosis and treatment of head and neck pathology. Although it has been validated in the investigation of thyroid pathology, there is a paucity of evidence to support its use in lateral neck masses. This study aims to determine the accuracy and adequacy of SUS‐FNA in the investigation of lateral neck masses.MethodsA retrospective cohort analysis was performed of patients who underwent a SUS‐FNA for lateral neck mass between June 2018 and October 2022 at a single institution. Pathologist reports were reviewed to determine the rate of FNA sample adequacy. A comparison was made between FNA cytology results and final histopathological diagnosis following surgical excision in a subset of patients to determine FNA accuracy.ResultsA total of 110 SUS‐FNAs were performed on lateral neck masses. Diagnostic adequacy of SUS‐FNA was determined to be 91% (100/110). When analysing the subset of patients who proceeded to surgical excision, the diagnostic accuracy of SUS‐FNA was determined to be 88% (38/43).ConclusionSUS‐FNA results in high adequacy rates with good diagnostic accuracy in the investigation of lateral neck masses. This tool has great potential in reducing treatment delay in the management of head and neck cancer.
Branchial CO2 and ammonia excretion in crustaceans: Involvement of an apical Rhesus‐like glycoprotein
Authors: Quijada‐Rodriguez Alex R. , Fehsenfeld Sandra , Marini Anna‐Maria , Wilson Jonathan M. , Nash Mikyla T. , Sachs Maria , Towle David W. , Weihrauch Dirk 
Published: January 2024

AbstractAimTo determine whether the crustacean Rh1 protein functions as a dual CO2/ammonia transporter and investigate its role in branchial ammonia excretion and acid–base regulation.MethodsSequence analysis of decapod Rh1 proteins was used to determine the conservation of amino acid residues putatively involved in ammonia transport and CO2 binding in human and bacterial Rh proteins. Using the Carcinus maenas Rh1 protein (CmRh1) as a representative of decapod Rh1 proteins, we test the ammonia and CO2 transport capabilities of CmRh1 through heterologous expression in yeast and Xenopus oocytes coupled with site‐directed mutagenesis. Quantitative PCR was used to assess the distribution of CmRh1 mRNA in various tissues. Western blotting was used to assess CmRh1 protein expression changes in response to high environmental ammonia and CO2. Further, immunohistochemistry was used to assess sub‐cellular localization of CmRh1 and a membrane‐bound carbonic anhydrase (CmCAg).ResultsSequence analysis of decapod Rh proteins revealed high conservation of several amino acid residues putatively involved in conducting ammonia transport and CO2 binding. Expression of CmRh1 in Xenopus oocytes enhanced both ammonia and CO2 transport which was nullified in CmRh1 D180N mutant oocytes. Transport of the ammonia analog methylamine by CmRh1 is dependent on both ionized and un‐ionized ammonia/methylamine species. CmRh1 was co‐localized with CmCAg to the apical membrane of the crustacean gill and only experienced decreased protein expression in the anterior gills when exposed to high environmental ammonia.ConclusionCmRh1 is the first identified apical transporter‐mediated route for ammonia and CO2 excretion in the crustacean gill. Our findings shed further light on the potential universality of dual ammonia and CO2 transport capacity of Rhesus glycoproteins in both vertebrates and invertebrates.
From Alzheimer's disease to vascular dementia: Different roads leading to cognitive decline
Authors: Semerano Aurora , Fernández‐Ruiz Javier , Cortes‐Canteli Marta , Moro María A. 
Published: January 2024

LINKED ARTICLESThis article is part of a themed issue From Alzheimer's Disease to Vascular Dementia: Different Roads Leading to Cognitive Decline. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v181.6/issuetoc
Moving together or left behind? An examination of rural migrant working mothers' childcare strategies in the cities
Authors: Guo Chunlan , Lin Liyue , Zhu Yu 
Published: January 2024

AbstractBackgroundIn China, an increasing number of rural mothers participate in urban labour markets, but little is known about their decisions regarding childcare while living in these cities. Why do some rural mothers migrate to the cities with their children, whereas others leave their children behind in the countryside?MethodsThis study analysed 1852 samples from the 2016 China Migrant Dynamic Survey of rural migrant mothers collected in the Pearl River Delta (PRD). These mothers were registered with agricultural hukou outside of the PRD and had at least one child under 18 years of age.ResultsThe results indicated that 57.8% of these mothers migrated together with their children. Rural migrant mothers who were self‐employed, had a higher level of household income on a log10 scale and had a longer duration of migration were more willing to adopt closely performing motherhood than rural migrant mothers who were not self‐employed. Additionally, rural working mothers who were intra‐provincial migrants and had a smaller number of children were more likely to bring their children to the cities than rural working mothers who were inter‐provincial migrants.ConclusionsThis study works to strengthen the understanding of rural migrant working mothers' childcare strategies, provide insights for future policy studies and contribute to evidence‐based recommendations for policymakers regarding internal rural‐to‐urban migration, migrant women and the wellbeing of the families of migrants.
Financial inclusion of Muslim minority in Vellore, Tamil Nadu
Authors: Ishtiyaq Nadeem Vavangani , Waheed Khalid , Fathima M.S Arshiya 
Published: January 2024

AbstractThe study aims to identify the potential determinants of financial inclusion and its impact on the Indian Muslim minority in Tamil Nadu. statistical package for social science and analysis of moment structures software packages were used in the study. Confirmatory factor analysis and structural equation modelling techniques were applied to examine the collected data (n = 300) from the Vellore district from April 2022 to March 2023. The study reveals that financial awareness, access and quality positively and significantly impact financial inclusion. However, the financial usage dimension is statistically insignificant. Additionally, the results from the moderation effect reveal that financial access and quality, along with age and annual income, significantly influence financial inclusion. In conclusion, the study contributes new dimensions to the existing literature by exploring the financial inclusion of Muslims and suggests implications and future scope for further research.
Soluble mannose receptor: A potential biomarker in Gaucher disease
Authors: Beaton Brendan , Hughes Derralynn A. 
Published: January 2024

AbstractPurposeSoluble mannose receptor (sMR) relates to mannose receptor expression on macrophages, and is elevated in inflammatory disorders. Gaucher disease (GD) has altered macrophage function and utilises mannose receptors for enzyme replacement therapy (ERT) endocytosis. sMR has not previously been studied in GD.MethodssMR was measured by ELISA and correlated with GD clinical features including spleen and liver volume, haemoglobin and platelet count, bone marrow burden (BMB) scores and immunoglobulin levels. sMR was compared with biomarkers of GD: chitotriosidase, lyso‐GL1, PARC, CCL3, CCL4, osteoactivin, serum ACE and ferritin.ResultsMedian sMR in untreated GD patients was 303.0 ng/mL compared to post‐treatment 190.9 ng/mL (p = .02) and healthy controls 202 ng/mL. Median sMR correlated with median spleen volume 455 mL (r = .70, p = .04), liver volume 2025 mL (r = .64, p = .04), BMB 7 (r = .8, p = .03), IgA 1.9 g/L (r = .54, p = .036), IgG 9.2 g/L (r = .57, p = .027), IgM 1.45 g/L (r = .86, p < .0001), with inverse correlation to median platelet count of 125 × 109/L (r = −.47, p = .08) and haemoglobin of 137 g/L (r = −.77, p = .0008). sMR correlated with established biomarkers: osteoactivin 107.8 ng/mL (r = .58, p = .0006), chitotriosidase 3042 nmol/mL/h (r = .52, p = .0006), PARC 800 ng/mL (r = .67, p = .0068), ferritin 547 μg/L (r = .72, p = .002) and CCL3 50 pg/mL (r = .67, p = .007).ConclusionssMR correlates with clinical features and biomarkers of GD and reduces following therapy.
Association of the neonatal Fc receptor promoter variable number of tandem repeat polymorphism with immunoglobulin response in patients with chronic inflammatory demyelinating polyneuropathy
Authors: Fisse Anna Lena , Schäfer Emelie , Hieke Alina , Schröder Maximilian , Klimas Rafael , Brünger Jil , Huckemann Sophie , Grüter Thomas , Sgodzai Melissa , Schneider‐Gold Christiane , Gold Ralf , Nguyen Huu Phuc , Pitarokoili Kalliopi , Motte Jeremias , Arning Larissa 
Published: January 2024

AbstractBackground and purposeChronic inflammatory demyelinating polyneuropathy (CIDP) is an autoimmune disease with humoral and cellular autoimmunity causing demyelination of peripheral nerves, commonly treated with intravenous immunoglobulins (IVIg). The neonatal Fc receptor (FcRn), encoded by the FCGRT gene, prevents the degradation of immunoglobulin G (IgG) by recycling circulating IgG. A variable number of tandem repeat (VNTR) polymorphism in the promoter region of the FCGRT gene is associated with different expression levels of mRNA and protein. Thus, patients with genotypes associated with relatively low FcRn expression may show a poorer treatment response to IVIg due to increased IVIg degradation.MethodsVNTR genotypes were analyzed in 144 patients with CIDP. Patients' clinical data, including neurological scores and treatment data, were collected as part of the Immune‐Mediated Neuropathies Biobank registry.ResultsMost patients (n = 124, 86%) were VNTR 3/3 homozygotes, and 20 patients (14%) were VNTR 2/3 heterozygotes. Both VNTR 3/3 and VNTR 2/3 genotype groups showed no difference in clinical disability and immunoglobulin dosage. However, patients with a VNTR 2 allele were more likely to receive subcutaneous immunoglobulins (SCIg) than patients homozygous for the VNTR 3 allele (25% vs. 9.7%, p = 0.02) and were more likely to receive second‐line therapy (75% vs. 54%, p = 0.05).ConclusionsThe VNTR 2/3 genotype is associated with the administration of SCIg, possibly reflecting a greater benefit from SCIg due to more constant immunoglobulin levels without lower IVIg levels between the treatment circles. Also, the greater need for second‐line treatment in VNTR 2/3 patients could be an indirect sign of a lower response to immunoglobulins.
Nitrogen deposition suppresses ephemeral post‐fire plant diversity
Authors: Valliere Justin M. , Irvine Irina C. , Allen Edith B. 
Published: January 2024

AbstractFire is a dominant force shaping patterns of plant diversity in Mediterranean‐type ecosystems. In these biodiversity hotspots, including California's endangered coastal scrub, many species remain hidden belowground as seeds and bulbs, only to emerge and flower when sufficient rainfall occurs after wildfire. The unique adaptations possessed by these species enable survival during prolonged periods of unfavorable conditions, but their continued persistence could be threatened by nonnative plant invasion and environmental change. Furthermore, their fleeting presence aboveground makes evaluating these threats in situ a challenge. For example, nitrogen (N) deposition resulting from air pollution is a well‐recognized threat to plant diversity worldwide but impacts on fire‐following species are not well understood. We experimentally evaluated the impact of N deposition on post‐fire vegetation cover and richness for three years in stands of coastal sage scrub that had recently burned in a large wildfire in southern California. We installed plots receiving four levels of N addition that corresponded to the range of N deposition rates in the region. We assessed the impact of pre‐fire invasion status on vegetation dynamics by including plots in areas that had previously been invaded by nonnative grasses, as well as adjacent uninvaded areas. We found that N addition reduced native forb cover in the second year post‐fire while increasing the abundance of nonnative forbs. As is typical in fire‐prone ecosystems, species richness declined over the three years of the study. However, N addition hastened this process, and native forb richness was severely reduced under high N availability, especially in previously invaded shrublands. An indicator species analysis also revealed that six functionally and taxonomically diverse forb species were especially sensitive to N addition. Our results highlight a new potential mechanism for the depletion of native species through the suppression of ephemeral post‐fire bloom events.