bluuebunny/crossref_metadata_2025_split huggingface dataset:
The utility of balloon‐based and standard impedance technology to diagnose esophageal mucosal diseases in children
Authors: Rosen Rachel , Du Maritha , Akkara Anna , Catacora Andrea , Wen Anna , Liu Enju , Nurko Samuel 
Published: December 2023

AbstractBackgroundStandard impedance catheters and balloon‐based mucosal impedance catheters (BBMI) have been used to assess mucosal integrity and diagnose mucosal diseases. The goal of this study was to determine the age‐related technical issues associated with mucosal balloon inflation, validate the BBMI measurement against a standard impedance probe, and compare software‐generated diagnoses to histologic diagnoses.MethodsWe prospectively recruited patients undergoing endoscopy, during which patients underwent standard mucosal impedance catheters and BBMI measurements. Measurements were compared to each other, to the histologic diagnoses, and to the number of eosinophils per high power field. We then compared the patients' diagnosis to that assigned by the BBMI software.Key ResultsSixty‐two patients (mean age: 62 ± 62 months) were recruited, including non‐GERD (N = 40), GERD (N = 15), and EoE (N = 7) patients. There were significant differences between the impedance values measured by the two technologies at each esophageal height (p < 0.003). There were significant correlations between the mean impedance values taken by the two catheters in the distal (r2 = 0.272, p = 0.04), mid (r2 = 0.371, p < 0.001), and proximal (r2 = 0.259, p = 0.05) esophagus. There were significant differences in BBMI impedance values across diagnoses in the mid and proximal esophagus (p = 0.024 and 0.025, respectively). While not statistically significant (p = 0.061–0.073), the standard catheter showed similar trends by diagnosis. Using the BBMI diagnostic prediction software, 33%–72% of patients were misclassified.Conclusion and InferencesWhile there was significant variability in impedance values between technologies within patients, regional measurements were consistent across catheters. Automated analyses lacked the sensitivity to diagnose inflammatory disorders.
PHB3 interacts with BRI1 and BAK1 to mediate brassinosteroid signal transduction in Arabidopsis and tomato
Authors: Li Cheng , Zhang Shan , Li Jingjuan , Huang Shuhua , Zhao Tong , Lv Siqi , Liu Jianwei , Wang Shufen , Liu Xiaohui , He Shen , Zhang Yanfeng , Xiao Fangming , Wang Fengde , Gao Jianwei , Wang Xiaofeng 
Published: December 2023

Summary
Brassinosteroids (BRs) are plant hormones that are essential in plant growth and development. BRASSINOSTEROID‐INSENSITIVE 1 (BRI1) and BRI1 ASSOCIATED RECEPTOR KINASE 1 (BAK1), which are located on the plasma membrane, function as co‐receptors that accept and transmit BR signals.
PROHIBITIN 3 (PHB3) was identified in both BRI1 and BAK1 complexes by affinity purification and LC‐MS/MS analysis. Biochemical data showed that BRI1/BAK1 interacted with PHB3 in vitro and in vivo.
BRI1/BAK1 phosphorylated PHB3 in vitro. When the Thr‐80 amino acid in PHB3 was mutated to Ala, the mutant protein was not phosphorylated by BRI1 and the mutant protein interaction with BRI1 was abolished in the yeast two‐hybrid assay. BAK1 did not phosphorylate the mutant protein PHB3T54A. The loss‐of‐function phb3 mutant showed a weaker BR signal than the wild‐type. Genetic analyses revealed that PHB3 is a BRI1/BAK1 downstream substrate that participates in BR signalling. PHB3 has five homozygous in tomato, and we named the closest to AtPHB3 as SlPHB3.1. Biochemical data showed that SlBRI1/SlSERK3A/SlSERK3B interacted with SlPHB3.1 and SlPHB3.3. The CRISPR‐Cas9 method generated slphb3.1 mutant led to a BR signal stunted relatively in tomatoes.
PHB3 is a new component of the BR signal pathway in both Arabidopsis and tomato.
Identification of six novel mutations in EDA from 20 hypohidrotic ectodermal dysplasia families
Authors: Xing Qin , Zhou Qimin , Li Hongyan , Wang Zhongjie , Li Shun , Wu Jiayu , Zhu Huimin , Liang Desheng , Li Zhuo , Wu Lingqian 
Published: December 2023

AbstractObjectiveTo investigate the genetic causes of 22 patients with clinically high suspicion of X‐linked hypohidrotic ectodermal dysplasia from 20 unrelated Chinese families, expand the spectrum of ectodysplasin‐A mutations, and provide more evidence for variants of uncertain significance.Subjects and MethodsWhole‐exome sequencing was performed and potentially pathogenic variants were verified by Sanger sequencing. Western blotting, real‐time PCR and immunofluorescence analyses were performed to investigate the preliminary functions of the candidate variants.ResultsNineteen ectodysplasin‐A variants were identified, six of which were not previously reported. Among these variants, we identified a patient who carried two mutations in ectodysplasin‐A and exhibited more severe phenotypes. Additionally, mutant protein expression levels decreased, whereas mRNA transcription levels increased. Cellular sublocalisation of the variants located in the tumour necrosis factor homologous domain showed that the proteins accumulated in the nucleus, whereas wild‐type proteins remained in the cell membrane. A rare indel variant and two classical splicing variants that lead to exon 7 skipping were detected.ConclusionsThis study provides definitive diagnoses for 20 families with suspected X‐linked hypohidrotic ectodermal dysplasia and additional information on clinical heterogeneity and genotype–phenotype relationships.
Tablet‐based tests of everyday visual function in a diabetic macular oedema (DME) clinic waiting area: A feasibility study
Authors: Taylor Deanna J. , Alquiza Paolo J. , Jones Pete R. , Wilson Iain , Bi Wei , Sim Dawn A. , Crabb David P. 
Published: December 2023

AbstractPurpose(1) To assess the feasibility of conducting tablet‐based vision tests in hospital clinic waiting areas; (2) To test the hypothesis that increasing severity of diabetic macular oedema (DME) is associated with the performance of tablet‐based surrogates of everyday tasks and self‐reported visual function.MethodsSixty‐one people with mild (n = 28), moderate (n = 24) or severe (n = 9) DME performed two tablet‐based tests of ‘real‐world’ visual function (visual search and face recognition) while waiting for appointments in a hospital outpatient clinic. Participants also completed a tablet‐based version of a seven‐item, visual‐functioning (VF‐7) patient‐reported outcome measure. Test performance was compared to previously published 99% normative limits for normally sighted individuals.ResultsThirty‐four participants (56%; 95% confidence interval [CI] 43%–68%) exceeded normative limits for visual search, while eight (13%; 95% CI 65%–24%) exceeded normative limits for face discrimination. Search duration was significantly longer for people with severe DME than those with mild and moderate DME (p = 0.01). Face discrimination performance was not significantly associated with DME severity. VF‐7 scores were statistically similar across DME severity groups. Median time to complete all elements (eligibility screening, both tablet‐based tasks and the VF‐7) was 22 (quartiles 19, 25) min. Further, 98% and 87% of participants, respectively, reported the search task and face discrimination task to be enjoyable, while 25% and 97%, respectively, reported finding the two tasks to be difficult.ConclusionsPortable tablet‐based tests are quick, acceptable to patients and feasible to be performed in a clinic waiting area with minimal supervision. They have the potential to be piloted in patients' homes for self‐monitoring.
Negative pressure suction test: An intraoperative airway maneuver to assess effectiveness of surgical correction of tracheobronchomalacia
Authors: Kuo Frederick H. , Elliott Richard A. , Watkins Scott C. , Shieh Hester F. , Smithers Charles J. , Jennings Russell W. , Munoz‐San Julian Carlos 
Published: December 2023

AbstractBackgroundSurgical correction of tracheobronchomalacia (TBM) has evolved greatly over the past decade, with select pediatric institutions establishing dedicated surgery and anesthesia teams to navigate the complexities and challenges of surgical airway repairs. Although anesthetic techniques have evolved internally over many years to improve patient safety and outcomes, many of these methods remain undescribed in literature.TechniqueIn this article, we describe the intraoperative negative pressure suction test. This simulates the negative pressure seen in awake and spontaneously breathing patients, including the higher pressures seen during coughing which induce airway collapse in patients with TBM. Also known as the Munoz maneuver in surgical literature, this test has been performed on over 300 patients since 2015.DiscussionThe negative pressure suction test allows for controlled intraoperative assessment of surgical airway repairs, replaces the need for risky intraoperative wake‐up tests, increases the chances of a successful surgical repair, and improves anesthetic management for emergence and extubation. We provide a guide on how to perform the test and videos demonstrating its efficacy in intraoperative airway evaluation.ConclusionsAs surgeries to repair TBM become more prevalent in other pediatric institutions, we believe that pediatric patients and anesthesia providers will benefit from the insights and methods described here.
Plasmodesmata‐associated Flotillin positively regulates broad‐spectrum virus cell‐to‐cell trafficking
Authors: Ge Panpan , Lu Hong , Wang Wei , Ma Yonghuan , Li Yi , Zhou Tong , Wei Taiyun , Wu Jianguo , Cui Feng 
Published: December 2023

SummaryViral diseases seriously threaten rice production. Plasmodesmata (PD)‐associated proteins are deemed to play a key role in viral infection in host plants. However, few PD‐associated proteins have been discovered in rice to afford viral infection. Here, inspired by the infection mechanism in insect vectors, we identified a member of the Flotillin family taking part in the cell‐to‐cell transport of rice stripe virus (RSV) in rice. Flotillin1 interacted with RSV nucleocapsid protein (NP) and was localized on PD. In flotillin1 knockout mutant rice, which displayed normal growth, RSV intercellular movement was retarded, leading to significantly decreased disease incidence. The PD pore sizes of the mutant rice were smaller than those of the wild type due to more callose deposits, which was closely related to the upregulation of two callose synthase genes. RSV infection stimulated flotillin1 expression and enlarged the PD aperture via RSV NP. In addition, flotillin1 knockout decreased disease incidences of southern rice black‐streaked dwarf virus (SRBSDV) and rice dwarf virus (RDV) in rice. Overall, our study reveals a new PD‐associated protein facilitating virus cell‐to‐cell trafficking and presents the potential of flotillin1 as a target to produce broad‐spectrum antiviral rice resources in the future.
Lucky artists
Authors: Prodoehl Christopher 
Published: December 2023

AbstractImagine an artist creating new work, a painter applying paint to canvas with a brush, for example. Assuming she acts intentionally, is she responsible for the work she creates? Is she responsible, in particular, for whatever value her finished work has? In the first part of the paper, I formulate an argument for the claim she is not; I call this the Luck Argument. According to that argument, an important aspect of the work's value is due to luck, so not something for which the artist is responsible. I then go on to challenge the Luck Argument. I suggest that intentional control is not the only type of control artists exercise over their bodily activity. There is another type, which I call receptive control. The concept of receptive control makes it possible to challenge a crucial premise in the Luck Argument.
The first three years: The association of early postpartum depressive symptoms with infant and toddler development
Authors: Glasser Saralee , Uziel Moshe , Wagman Shir , Zaworbach Hani , Ferber Yona , Levinson Daphna , Lerner‐Geva Liat 
Published: December 2023

AbstractBackgroundThe influence of postpartum depression (PPD) on child development has been a source of professional interest and practical relevance.ObjectiveThis study investigated the association of early PPD symptoms with developmental domains.Design and methodThis historical cohort study included 574,282 children attending Mother Child Healthcare Centers in Israel from January 1, 2014 to July 31, 2020, who underwent at least one developmental screening examination by public health nurses up to age 36 months, and whose mothers completed the Edinburgh Postnatal Depression Scale (EPDS) postnatally. Developmental milestone tasks included four domains: fine and gross motor, language/communication, and social/behavioral.ResultsThe rate of failure to complete age‐appropriate tasks was higher among children whose mothers had scored ≥ 10 on the EPDS on the majority of tasks in every domain.DiscussionThis large population‐based study has demonstrated the association between early maternal postnatal depressive symptoms and failure to meet developmental milestones across domains, until three years. Recommendations for practice focus on the mother, the child, and health policy.
Multivariate analysis of the effects of weather variables on white rust epidemics and yield reduction of mustard over multiple growing seasons
Authors: Singh Manjari , Savary Serge , Setiya Parul , Tewari Anand Kumar , Nain Ajeet Singh 
Published: December 2023

AbstractThree shrinkage regression and two machine‐learning approaches were evaluated to derive models for the prediction of epidemiological characteristics of white rust of mustard, using data from 112 epidemics in the field. Four epidemiological characteristics were considered: (a) crop age at first appearance of disease, (b) crop age at highest disease severity, (c) highest disease severity in a growing season and (d) area under disease progress curve (AUDPC), along with (e) crop yield to measure the effects of disease on crop performance. We developed models using weather indices to predict these variables using five different approaches: ANN, Elastic Net, LASSO, random forest and ridge regression. One model was developed for each sowing date corresponding to each dependent variable. Two hundred different models were developed. All models performed well at the calibration stage for most of the five variables at all sowing dates. However, at the validation stage, ANN‐derived models outperformed (R2val ~ 1.00, nRMSEV ~0.00 and MBEV ~0.00 in most cases) the three shrinkage regression‐derived models in predicting all five variables. Predictions by random forest‐ and LASSO‐derived models were acceptable for AUDPC and crop yield. Evaluation metrics (including R2val, nRMSEV and MBEV) suggested that ENET‐ and ridge‐derived models do not perform satisfactorily, whereas ANN‐derived models yielded reliable results and thus generate robust predictions. The present work constitutes a systematic effort to compare modelling methods for disease and yield prediction and illustrates the relevance of weather variables in predicting multiple epidemiological variables, and of multiple disease variables as predictors of actual crop yield.
Integrated multi‐omic data and analyses reveal the response pathways of green onion (Allium fistulosum L.) to high‐temperature stress
Authors: Guo Yuanyuan , Zhang Li , Li Yang , Chen Qin , Wen Junli , Tang Juan , Song Huanzhong , Liu Touming , Lv Bingsheng , Kang Dexian , Gao Song , Chen Zhendong 
Published: December 2023

AbstractWith the intensification of the greenhouse effect and the continuous rise of global temperature, high temperatures in summer seriously affect the growth of green onion (Allium fistulosum L.var.caespitosum Makino) and reduce its yield and quality. It is important to study the mechanism of heat tolerance in green onion for selecting and breeding new varieties with high‐temperature tolerance. In this study, we used the heat‐tolerant green onion variety AF60 and heat‐sensitive green onion variety AF35 and measured their physiological indexes under different durations of heat stress. The results showed that high‐temperature stress adversely affected the water content, protein composition and antioxidant system of green onion. In addition, a comprehensive analysis using transcriptomics and metabolomics showed that heat‐tolerant green onions responded positively to heat stress by up‐regulating the expression of heat shock proteins, whereas heat‐sensitive green onions responded to heat stress by activating the galactose metabolic pathway and maintained normal physiological activities. This study revealed the physiological performance and high‐temperature response pathways of different heat‐tolerant green onion cultivars under heat stress. The results further deepen the understanding of the molecular mechanism of green onion's heat stress response.
Insights into the molecular mechanism of Trichoderma stimulating plant growth and immunity against phytopathogens
Authors: Khan Raja Asad Ali , Najeeb Saba , Chen Jie , Wang Rui , Zhang Jing , Hou Jumei , Liu Tong 
Published: December 2023

AbstractTrichoderma species have received significant interest as beneficial fungi for boosting plant growth and immunity against phytopathogens. By establishing a mutualistic relationship with plants, Trichoderma causes a series of intricate signaling events that eventually promote plant growth and improve disease resistance. The mechanisms contain the indirect or direct involvement of Trichoderma in enhancing plant growth by modulating phytohormones signaling pathways, improving uptake and accumulation of nutrients, and increasing soil bioavailability of nutrients. They contribute to plant resistance by stimulating systemic acquired resistance through salicylic acid, jasmonic acid, and ethylene signaling. A cascade of signal transduction processes initiated by the interaction of Trichoderma and plants regulate the expression of defense‐related genes, resulting in the synthesis of defense hormones and pathogenesis‐related proteins (PRPs), which collectively improve plant resistance. Additionally, advancements in omics technologies has led to the identification of key pathways, their regulating genes, and molecular interactions in the plant defense and growth promotion responses induced by Trichoderma. Deciphering the molecular mechanism behind Trichoderma's induction of plant defense and immunity is essential for harnessing the full plant beneficial potential of Trichoderma. This review article sheds light on the molecular mechanisms that underlie the positive effects of Trichoderma‐induced plant immunity and growth and opens new opportunities for developing environmentally friendly and innovative approaches to improve plant immunity and growth.
The very possibility of contemplation: The dialectics of intellect and will in Schopenhauer's aesthetics
Authors: Sattar Alexander 
Published: December 2023

AbstractIn this article, I explore how Schopenhauer's theory of aesthetic experience—independently of his theory of arts—accommodates the possibility of contemplation. The standard reading of his aesthetics is that contemplation becomes possible because of a certain “surplus” of intellect and facilitating external occasions. I argue, however, that the “essential imperfections” of intellect and Schopenhauer's overall metaphysics are inconsistent with the very idea of will‐less cognition and, hence, of a free intellect. An alternative explanation of contemplation better fits with Schopenhauer's philosophy overall by stating that it is driven by the will itself. I analyze two approaches to elaborating such a theory. First, the “metaphysical” account, according to which the will as thing‐in‐itself brings forth the (surplus of) intellect needed for contemplation as a means of self‐cognition. Second, the “physiological” account, which presupposes a quasi‐evolutionary process that has aesthetic cognition as a byproduct of intellect. The two accounts complement each other and in tandem better explain the possibility of contemplation than postulating a will‐less intellect.
COVID‐19 responsibility and blame: How group identity and political ideology inform perceptions of responsibility, blame, and racial disparities
Authors: Wallace Lyndsey , Mikkelborg Anna , Gonzales Rubi , Hurd Kyneshawau , Romano Celina , Plaut Victoria 
Published: December 2023

AbstractThis study explored how racial group, racial identity centrality, and political ideology inform perceptions of responsibility, blame, and racial disparities in COVID‐19 outcomes. The findings revealed that highly identified members of non‐dominant racial groups were less likely to endorse items indicating individual blame, while being more inclined to attribute racial disparities to structural inequalities. Furthermore, conservative ideology was consistently linked to individual blame and responsibility, with those endorsing conservative ideology agreeing less with explanations of racial disparities based on structural inequalities and agreeing more with explanations for racial disparities based on personal blame and responsibility. Understanding perceptions of blame and responsibility for COVID‐19 may influence political discourse and subsequent health inequities, highlighting the importance of exploring how race, identity, and political ideology shape such perceptions.
An artificial neural network for digital image correlation dynamic subset selection based on speckle pattern quality metrics
Authors: Atkinson Devan James , van Rooyen Melody , Becker Thorsten Hermann 
Published: December 2023

AbstractPrecise and accurate digital image correlation computed displacement data requires sufficient noise suppression and spatial resolution, which improve and diminish, respectively, with increased subset size. Furthermore, spatially varying speckle pattern quality and displacement field complexity ideally necessitate a location‐specific optimal subset size to obtain a favourable compromise between noise suppression and spatial resolution. Although dynamic subset selection (DSS) methods have been proposed based on speckle pattern quality metrics (SPQMs), they do not ensure such a favourable compromise.This work investigates using an artificial neural network (ANN) for DSS. An ANN is trained to predict the displacement error standard deviation of a subset from multiple SPQMs and the standard deviation of image noise, such that the smallest subset offering sufficient noise suppression, dictated by a displacement error standard deviation threshold, is appointed.Validation, both within and outside the domain of the training images, shows that the smallest subset providing sufficient noise suppression offers a favourable compromise for up to moderate displacement gradients. Additionally, the proposed method is shown to perform with greater consistency and reliability relative to existing SPQM‐based DSS methods.The novel proposition lies in utilising an ANN as an error prediction tool, based on multiple SPQMs, and hence, is an attractive alternative for DSS.