bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reducing stomatal density by expression of a synthetic EPF increases leaf intrinsic water use efficiency and reduces plant water use in a C4crop
Authors: Ferguson John N. , Schmuker Peter , Dmitrieva Anna , Quach Truyen , Zhang Tieling , Ge Zhengxiang , Nersesian Natalya , Sato Shirley J , Clemente Tom E. , Leakey Andrew D.B. 
Published: February 2024

AbstractEnhancing crop water use efficiency (WUE) is a key target trait for climatic resilience and expanding cultivation on marginal lands. Reducing stomatal conductance (gs) through manipulating stomatal density has been observed to translate to improved WUE in multiple C3crop species. However, reducinggsin C3species often reduces photosynthetic carbon gain. A different response is expected in C4plants because they possess specialized anatomy and biochemistry which concentrates CO2at the site of fixation. This modifies the photosynthesis (AN) relationship with intracellular CO2concentration (ci) so that photosynthesis is CO2-saturated and reductions ingsare unlikely to impairAN. To test this hypothesis, genetic strategies were investigated to reduce stomatal density in the C4crop sorghum. Constitutive expression of a synthetic epidermal patterning factor (EPF) transgenic allele in sorghum, lead to reduced stomatal densities. A moderate reduction in stomatal density did not strengthen stomatal limitation to AN, improved WUE, reduced water use, and avoided loss of carbon fixation during a period of water deprivation. However, these positive outcomes were associated with negative pleiotropic effects on reproductive development and photosynthetic capacity. Avoiding pleiotropy by targeting expression of the transgene to specific tissues provides a potential pathway to optimal agronomic outcomes.
Hospital Care Trajectories of Older Adults with Cancer and the Associated Clinical Profiles: linking a French Prospective Cohort Study and a Clinical Data Warehouse
Authors: Jean Charline , Paillaud Elena , Boudou-Rouquette Pascaline , Martinez-Tapia Claudia , Pamoukdjian Frédéric , Hagège Meoïn , Bréant Stéphane , Hassen-Khodja Claire , Natella Pierre-André , Cudennec Tristan , Laurent Marie , Caillet Philippe , Audureau Etienne , Canouï-Poitrine Florence 
Published: February 2024

AbstractBackgroundThe longitudinal hospital care experiences of older adults with cancer, from the treatment decision-making process until their end of life, remain unexplored. We examined the hospital care trajectories of these patients and identified associated clinical determinants.MethodsWe linked the ELCAPA multicenter cohort study (patients aged ≥70 with a solid tumor and having been referred for a geriatric assessment between 2012 and 2019) and the Greater Paris University Hospitals’ clinical data warehouse. Individual care trajectories, defined as series of consultations, hospital admissions (in day, acute or rehabilitation units) and emergency room visits, were clustered using multichannel sequence analysis. Cluster membership determinants were identified among socio-demographic, oncological and geriatric parameters by logistic regression analysis.Results707 patients (median age: 82; metastatic cancer: 45.2%; 10,998 care episodes) were included. Four trajectory clusters were identified: cluster A (n=149, 21.1%) with in-hospital surgical trajectories, cluster B (n=198, 28.0%) with outpatient care trajectories with chemotherapy and/or radiotherapy, cluster C (n=302, 42.7%) without any hospital cancer treatments, and cluster D (n=58, 8.2%) with mostly chemotherapy and high hospital care consumption. Cluster belonging determinants included metastatic status and cancer site (for cluster A), cognition, mobility and mood status (unimpaired parameters for cluster B and impaired for cluster C), and younger age (for cluster D).ConclusionWhile highlighting varied hospital care experiences among older patients with cancer, we found that age remains an independent determinant of chemotherapy-dominant care trajectories.
Dissecting quantitative trait nucleotides by saturation genome editing
Authors: Roy Kevin R. , Smith Justin D. , Li Shengdi , Vonesch Sibylle C. , Nguyen Michelle , Burnett Wallace T. , Orsley Kevin M. , Lee Cheng-Sheng , Haber James E. , St.Onge Robert P. , Steinmetz Lars M. 
Published: February 2024

AbstractGenome editing technologies have the potential to transform our understanding of how genetic variation gives rise to complex traits through the systematic engineering and phenotypic characterization of genetic variants. However, there has yet to be a system with sufficient efficiency, fidelity, and throughput to comprehensively identify causal variants at the genome scale. Here we explored the ability of templated CRISPR editing systems to install natural variants genome-wide in budding yeast. We optimized several approaches to enhance homology-directed repair (HDR) with donor DNA templates, including donor recruitment to target sites, single-stranded donor production by bacterial retrons, and in vivo plasmid assembly. We uncovered unique advantages of each system that we integrated into a single superior system named MAGESTIC 3.0. We used MAGESTIC 3.0 to dissect causal variants residing in 112 quantitative trait loci across 32 environmental conditions, revealing an enrichment for missense variants and loci with multiple causal variants. MAGESTIC 3.0 will facilitate the functional analysis of the genome at single-nucleotide resolution and provides a roadmap for improving template-based genome editing systems in other organisms.
Temperature-induced changes in protein interactions control RNA recruitment to G3BP1 condensates
Authors: Fischer Charlotte M. , Ausserwöger Hannes , Sneideris Tomas , Qian Daoyuan , Scrutton Rob , Qamar Seema , St George-Hyslop Peter , Knowles Tuomas P. J. 
Published: February 2024

AbstractBiomolecular condensates have emerged as prominent regulators of dynamic subcellular organisation and essential biological processes. Temperature, in particular, exerts a significant influence on the formation and behaviour of biomolecular condensation. For example, during cellular heat stress, stress granules (SGs) are formed from RNA-binding proteins (RBPs) and RNA, forming liquid condensates to protect the RNA from damage. However, the molecular mechanisms leading to changes in protein phase behaviour are not well understood. To answer how temperature modulates protein interactions and phase behaviour, we developed a high-throughput microfluidic platform, capable of mapping the phase space and quantifying protein interactions in a temperature-dependent manner. Specifically, our approach measures high-resolution protein phase diagrams as a function of temperature, while accurately quantifying changes in the binodal, condensate stoichiometry and free energy contribution of a solute, hence, providing information about the underlying mechanistic driving forces. We employ this approach to investigate the effect of temperature changes on the phase separation of the stress granule scaffold protein Ras GTPase-activating protein-binding protein 1 (G3BP1) with PolyA-RNA. Surprisingly, we find that the G3BP1/RNA phase boundary remains unaffected by the increasing temperature but the underlying stoichiometry and energetics shift, which can only be revealed with high-resolution phase diagrams. This indicates that temperature-induced dissolution is counteracted by entropic processes driving phase separation. With increasing temperature, the G3BP1 content in condensates decreases alongside with a reduction of the free energy of protein interactions, while the RNA content increases driven by entropically favoured hydrophobic interactions. In the context of cellular heat SG formation, these findings could indicate that during heat shock, elevated temperatures directly induce RNA recruitment to stress granules as a cytoprotective mechanism by finetuning the strength of protein and RNA interactions.
Dynamics of the membrane- and cell wall-associated proteome ofArabidopsis thalianaroots in response to uranium stress
Authors: Przybyla-Toscano Jonathan , Chetouhi Cherif , Balliau Thierry , Alban Claude , Bourguignon Jacques , Ravanel Stéphane 
Published: February 2024

ABSTRACTUranium (U) is a non-essential and toxic metal for plants, which have the ability to take up uranyl ions from the soil and preferentially accumulate them in the roots. We showed that the bulk of the radionuclide accumulates in the root insoluble proteome of Arabidopsis plants challenged with U. Therefore, to elucidate new molecular mechanisms related to U stress response and tolerance, we used label-free quantitative proteomics to analyze the dynamics of the root membrane- and cell wall-enriched proteome under U stress. Of the 2,802 proteins identified, 458 showed differential accumulation in response to U. Biological processes affected by U include response to stress, amino acid metabolism, and previously unexplored functions associated with membranes and the cell wall. Indeed, our analysis supports a dynamic and complex reorganization of the cell wall in response to U stress, including lignin and suberin synthesis, pectin modifications, polysaccharide hydrolysis, and Casparian strips formation. Water flux through aquaporins and vesicular trafficking were also significantly perturbed by U stress. Finally, the abundance of metal transporters and iron, calcium, and other metal-binding proteins was affected by U. These proteins may play a role in controlling the fate and toxicity of U in plants.
Grapevine cell response to carbon deficiency requires transcriptome and methylome reprogramming
Authors: Berger Margot M J , Garcia Virginie , Lacrampe Nathalie , Rubio Bernadette , Decros Guillaume , Pétriacq Pierre , Flandin Amélie , Cassan Cédric , Hilbert-Masson Ghislaine , Colombié Sophie , Atanassova Rossitza , Gallusci Philippe 
Published: February 2024

ABSTRACTSugar limitation has dramatic consequences on plant cells, which include a profound reorganization of the cell metabolism, transcriptional reprogramming, and the recycling of cellular components to maintain fundamental cell functions. There is so far no description of the possible contribution of epigenetic regulations in the adaptation of plant cells to limited carbon availability. We investigated this question using non-photosynthetic grapevine cells (Vitis vinifera, cv Cabernet Sauvignon) culturedin vitrowith contrasted glucose concentrations. As expected, limited sugar availability in the culture medium led to a rapid cell growth arrest. This was associated with a major metabolic shift characterized by depletion in soluble sugar and total amino acids, an increase in malate content and changes in the cell redox status. Consistently, flux modeling showed a dramatic slowdown of many pathways required for biomass accumulation such as cell wall polymers and total protein content. In contrast, anaplerotic fluxes, the synthesis of some amino acids, redox and polyamine metabolism were enhanced.Carbon deprivation also resulted in a major transcriptional reprogramming characterized by the induction of genes involved in photosynthesis, and the repression of those related to sucrose mobilization or cell cycle control. Similarly, the epigenetic landscape was deeply modified. Glucose-depleted cells showed a higher global DNA methylation level than those grown with glucose. Changes in DNA methylation mainly occurred at transposable elements, but also at genes including differentially expressed genes, suggesting that DNA methylation could participate in the adaptation of cells to limited sugar availability. In addition, genes encoding histone modifiers were differentially expressed suggesting that additional epigenetic mechanisms may be at work during the response of plant cells to carbon shortage.
Translational arrest and mRNA decay are independent activities of alphaherpesvirus virion host shutoff proteins
Authors: Eke Lucy , Tweedie Alistair , Cutts Sophie , Wise Emma L , Elliott Gillian 
Published: February 2024

SummaryThe herpes simplex virus 1 (HSV1) virion host shutoff (vhs) protein is an endoribonuclease that regulates the translational environment of the infected cell, by inducing the degradation of host mRNA via cellular exonuclease activity. To further understand the relationship between translational shutoff and mRNA decay, we have used ectopic expression to compare HSV1 vhs (vhsH) to its homologues from four other alphaherpesviruses – varicella zoster virus (vhsV), bovine herpesvirus 1 (vhsB), equine herpesvirus 1 (vhsE) and Marek’s disease virus (vhsM). Only vhsH, vhsB and vhsE induced degradation of a reporter luciferase mRNA, with polyA+in situhybridisation indicating a global depletion of cytoplasmic polyA+ RNA and a concomitant increase in nuclear polyA+ RNA and the polyA tail binding protein PABPC1 in cells expressing these variants. By contrast, vhsV and vhsM failed to induce reporter mRNA decay and polyA+ depletion, but rather, induced cytoplasmic G3BP1 and polyA+ mRNA-containing granules and phosphorylation of the stress response proteins eIF2α and protein kinase R. Intriguingly, regardless of their apparent endoribonuclease activity, all vhs homologues induced an equivalent general blockade to translation as measured by single cell puromycin incorporation. Taken together, these data suggest that the activities of translational arrest and mRNA decay induced by vhs are separable and we propose that they represent sequential steps of the vhs host interaction pathway.
Green marketing mix and repurchase intention: the role of green knowledge
Authors: Mahmoud Mahmoud Abdulai , Seidu Alimatu Sadia , Tweneboah-Koduah Ernest Yaw , Ahmed Abdul Salam 
Published: February 2024

PurposeThis study investigated the effect of green marketing mix on consumer repurchase intention in Ghana. The study focusses on the interaction effect of green knowledge on green marketing mix and consumer repurchase in Ghana.Design/methodology/approachA quantitative approach to research was employed. In all, 371 participants were chosen using the purposive sampling technique. Data analysis was conducted using the SPSS software.FindingsThe findings showed that green price, green place and green promotion had a positive significant effect on repurchase intention. However, green product insignificantly influenced repurchase intention. The findings further showed that green knowledge moderated the relationship between green price and green place, on repurchase intention. Green knowledge was not found to moderate the relationship between green product, green promotion and repurchase intention.Originality/valueThe study advances our knowledge on green marketing mix, green knowledge and repurchase intention within the beverage sector. It reveals the positive implication of green marketing mix on a firm’s customers using the marketing mix theory.
Damping accumulated discrete MGM(1, m) power model and its application to forecasting agricultural output value share and employment share
Authors: Li Liangshuai , Luo Dang 
Published: February 2024

PurposeThe damping accumulated discrete MGM(1, m) power model is proposed for the problem of forecasting the share of agricultural output value and the share of employment in China.Design/methodology/approachIn this study, the damping accumulated discrete MGM(1, m) power model was developed based on the idea of discrete modelling by introducing a damping accumulated generating operator and power index. The new model can better identify the non-linear characteristics existing between different factors in the multivariate system and can accurately describe and forecast the trend of changes between data series and each of them.FindingsThe validity and rationality of the new model are verified through numerical experiment. It is forecasted that in 2023, the share of agricultural output value in China will be 7.14% and the share of agricultural employment will be 21.98%, with an overall decreasing trend.Practical implicationsThe simultaneous decline in the share of agricultural output value and the share of employment is a common feature of countries that have achieved agricultural modernisation. Accurate forecasts of the share of agricultural output value and the share of employment can provide an important scientific basis for formulating appropriate agricultural development targets and policies in China.Originality/valueThe new model proposed in this study fully considers the importance of new information and has higher stability. The differential evolutionary algorithm was used to optimise the model parameters.
Investigating the effect of learning styles on HyFlex learning towards equity of learning in higher education
Authors: Mahande Ridwan Daud , Abdal Nurul Mukhlisah , Nasir Nasir 
Published: February 2024

Purpose
This paper aims to investigate the effect of learning styles on HyFlex learning towards equity of learning in higher education.

Design/methodology/approach
A quantitative approach was used, with data collection through a structured online questionnaire. The study participants were undergraduate students (n = 451) studying at various public and private universities in Indonesia. Measurement analysis is used to test the validity of the instrument used. Analysis of structural equations is used to test the relationships between the constructs under study.

Findings
Survey instruments have satisfactory internal validity and consistency. The learning style of students in higher education positively influences the use of HyFlex’s three learning modalities. All three modalities of HyFlex learning positively affect learning equity, especially the asynchronous online modality. However, the synchronous online effect is insignificant. Active/reflective learning styles only affect face-to-face mode but do not significantly affect the two online modalities, synchronous and asynchronous. Some of the learning style dimensions have an indirect effect on equity through three HyFlex learning modalities. Face-to-face and online asynchronous mediate well the indirect relationship between learning style and equity. The impact of gender and higher education status was not shown to strengthen the relationship between learning styles, HyFlex learning modalities and equity.

Research limitations/implications
This study will provide valuable understanding for lecturers, educators and developers to adapt and develop HyFlex learning strategies based on the positive dimensions of the Felder–Silverman learning style that can support equitable and inclusive learning. The study forms a foundation for researchers to investigate more constructs that could improve HyFlex learning in future studies.

Originality/value
This research is a pioneer in using learning styles to investigate trends in using three HyFlex learning modalities, particularly emphasising modalities that can provide equitable learning.
Competitiveness, quality education and universities: the shift to the post-pandemic world
Authors: Arredondo-Trapero Florina Guadalupe , Guerra-Leal Eva María , Kim Joohee , Vázquez-Parra José Carlos 
Published: February 2024

PurposeThis article aims to investigate whether there is a relationship between education for the labor market in the post-pandemic stage and the educational quality of universities, taking as a sample a group of Latin American countries and their main trading partners. Reference is made to the Global Competitiveness Report 2020 of the World Economic Forum (WEF) and the Quacquarelli Symonds (QS) index, which evaluates the quality of universities.Design/methodology/approachCorrelations, clusters and T-tests were generated to test for significant differences, resulting in two blocks of countries being identified with statistically significant differences in educational efforts to prepare their citizens for the labor market and the quality of their universities.FindingsIt is concluded that there are examples of Latin American countries that, although they are emerging economies, are updating their educational systems at an accelerated pace to meet the needs demanded by the labor market, such as Chile and Argentina. In addition, there are some particular cases of Latin American QS universities that although they are not at a high level in their overall educational quality as a university, compared to North American and Asian universities, their graduates do show a high employability index. This means that in the graduate profile, these universities are also accelerating efforts to position them at a high level of preparedness to respond to the jobs and markets of tomorrow, just as the universities in developed economies do.Research limitations/implicationsAlthough the sample size is a limitation of this work, since it is based on secondary information reported by the WEF (2020) and the QS World University Rankings (2021), it contributes value by analyzing specific cases. Despite its limitations, the study yields meaningful results that put the challenges of post-pandemic employment and the role that universities play in a comprehensible framework.Originality/valueThese results put special attention on the work of universities as a crucial entity to prepare citizens to develop the competencies needed for the post-pandemic labor market, especially in terms of critical thinking and digital skills. Human capital formation will drive the post-pandemic recovery process of leading countries and universities.
From parameters to policies: sensitivity analysis and fiscal and monetary reactions
Authors: Marques Junior Karlo 
Published: February 2024

PurposeThis paper seeks to explore the sensitivity of these parameters and their impact on fiscal policy outcomes. We use the existing literature to establish possible ranges for each parameter, and we examine how changes within these ranges can alter the outcomes of fiscal policy. In this way, we aim to highlight the importance of these parameters in the formulation and evaluation of fiscal policy.Design/methodology/approachThe role of fiscal policy, its effects and multipliers continues to be a subject of intense debate in macroeconomics. Despite adopting a New Keynesian approach within a macroeconomic model, the reactions of macroeconomic variables to fiscal shocks can vary across different contexts and theoretical frameworks. This paper aims to investigate these diverse reactions by conducting a sensitivity analysis of parameters. Specifically, the study examines how key variables respond to fiscal shocks under different parameter settings. By analyzing the behavioral dynamics of these variables, this research contributes to the ongoing discussion on fiscal policy. The findings offer valuable insights to enrich the understanding of the complex relationship between fiscal shocks and macroeconomic outcomes, thus facilitating informed policy debates.FindingsThis paper aims to investigate key elements of New Keynesian Dynamic Stochastic General Equilibrium (DSGE) models. The focus is on the calibration of parameters and their impact on macroeconomic variables, such as output and inflation. The study also examines how different parameter settings affect the response of monetary policy to fiscal measures. In conclusion, this study has relied on theoretical exploration and a comprehensive review of existing literature. The parameters and their relationships have been analyzed within a robust theoretical framework, offering valuable insights for further research on how these factors influence model forecasts and inform policy recommendations derived from New Keynesian DSGE models. Moving forward, it is recommended that future work includes empirical analyses to test the reliability and effectiveness of parameter calibrations in real-world conditions. This will contribute to enhancing the accuracy and relevance of DSGE models for economic policy decision-making.Originality/valueThis study is motivated by the aim to provide a deeper understanding of the roles macroeconomic model parameters play concerning responses to expansionary fiscal policies and the subsequent reactions of monetary authorities. Comprehensive reviews that encompass this breadth of relationships within a single text are rare in the literature, making this work a valuable contribution to stimulating discussions on macroeconomic policies.