bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age-Related Changes in Total Corneal Astigmatism in Eyes With High Myopia
Authors: Hayashi Ken , Uno Koichi , Manabe Shin-ichi , Yoshimura Koichi 
Published: December 2023

Purpose:
The purpose of this study was to compare age-related changes in corneal astigmatism in eyes with and without high myopia.

Methods:
Eight-hundred eyes with high myopia (axial length ≥26.0 mm) and 800 eyes without high myopia (200 eyes each from patients in their 40s, 50s, 60s, and ≥70s) underwent videokeratographic examination. The amounts of vertical/horizontal (Rx) and oblique astigmatism (Ry) components, irregular astigmatism, and corneal shape were compared between eyes with and without high myopia and among age categories.

Results:
In both groups, the mean Rx significantly changed to more positive with age (P < 0.001), whereas the Ry did not change significantly. The Rx was significantly more negative in the high myopia group than in the control group in all age categories (P ≤ 0.003), whereas the Ry did not differ significantly. The mean changes in the Rx and Ry during each 2 consecutive decades did not differ significantly between groups. The asymmetry and higher-order irregularity components increased with age (P ≤ 0.001) but did not differ significantly between groups, except for the higher-order irregularity in patients in their 60s (P = 0.018). In the averaged map, the corneal shape changed from with-the-rule to against-the-rule astigmatism with age in both groups, but the changes occurred later in the high myopia group.

Conclusions:
Age-related changes from with-the-rule to against-the-rule astigmatism occurred later in eyes with high myopia compared with eyes without high myopia in middle or older aged patients, but this change in each age decade was comparable between eyes with and without high myopia.
Nurse Leader Attitudes and Beliefs Regarding Medical Errors
Authors: Prothero Marie M. , Huefner Katherine , Sorhus Madeline 
Published: December 2023

OBJECTIVE
This study aimed to explore nurse leader attitudes and beliefs about medical errors.

BACKGROUND
The perfectibility model suggests errors are avoidable if nurses are trained and follow safety guidelines. This impacts how nurse leaders approach medical errors. Nurse leaders (NLs) may be the first person to whom a nurse reports an error. Leaders support nurses through the investigation process and subsequent recovery.

METHODS
A cross-sectional quantitative study was conducted to determine NL attitudes toward medical errors using the Medical Error Attitude Scale (MEAS).

RESULTS
MEAS scores were high in all categories. Medical error perception was significant for NLs with institutional support programs and when compared with job titles. Medical error approach and perception were significant for NLs with direct reports who had committed a medical error.

CONCLUSION
This study provides insight into NLs' views on medical errors, awareness of NLs who have had nurses involved in medical errors, and formal caregiver support programs.
18F-FDG PET/CT in Renal Allograft Tuberculosis Mimicking as Posttransplant Malignancy
Authors: Moideen Amal , Singh Harmandeep , Kumar Rajender , Naik Sachin Motiram , Gupta Anna , Sekar Aravind , Nada Ritambhra 
Published: December 2023

Abstract
The most common complications after the renal transplant are infections and malignancies, including posttransplant lymphoproliferative disorders. Tubercular infection in renal allograft recipients is a relatively rare entity. However, nonspecific constitutional symptoms often delay diagnosis, leading to significant morbidity and mortality. We present the 18F-FDG PET/CT findings in a patient with renal allograft tuberculosis who had clinical and imaging suspicion of posttransplant lymphoproliferative disorder or renal cell carcinoma. Histopathology from the renal lesion revealed tuberculosis.
Incidental Detection of Urinary Leakage on FDG PET/CT Imaging
Authors: Hou Naifeng , Fan Chengzhong , Shen Guohua 
Published: December 2023

Abstract
A 57-year-old woman previously diagnosed as cervical cancer underwent an 18F-FDG PET/CT scan for restaging. A pelvic encapsulated effusion with intense FDG accumulation was incidentally revealed. The tracer activity was similar to that of urine, indicating presence of urinary leakage. Subsequent CT urography confirmed the complete avulsion of the right distal ureter.
Combined Liver Transplant and Cardiac Surgery
Authors: Wehrle Chase J , Schlegel Andrea , Khalil Mazhar , Rotroff Daniel , Del Prete Luca , Maspero Marianna , Raj Roma , Frankel William C , Eghtesad Bijan , Aucejo Federico , Fujiki Masato , Kwon Choon David , Kim Jaekeun , Tong Michael Z Y , Unai Shinya , Cywinski Jacek , Modaresi Esfeh Jamak , Fares Maan , Pita Alejandro , Miller Charles , Quintini Cristiano , Hashimoto Koji , Diago-Uso Teresa 
Published: December 2023

Objective:
We aim to report our institutional outcomes of single-staged combined liver transplantation (LT) and cardiac surgery (CS).

Summary Background Data:
Concurrent LT and CS is a potential treatment for combined cardiac dysfunction and end-stage liver disease, yet only 54 cases have been previously reported in the literature. Thus, the outcomes of this approach are relatively unknown, and this approach has been previously regarded as extremely risky.

Methods:
Thirty-one patients at our institution underwent combined cardiac surgery and liver transplant. Patients with at least one-year follow-up were included. The Leave-One-Out Cross-Validation (LOOCV) machine-learning approach was used to generate a model for mortality.

Results:
Median follow-up was 8.2 years (IQR 4.6-13.6 y). One- and five-year survival was 74.2% (N=23) and 55% (N=17), respectively. Negative predictive factors of survival included recipient age>60 years (P=0.036), NASH-cirrhosis (P=0.031), Coronary Artery Bypass-Graft (CABG)-based CS (P=0.046) and pre-operative renal dysfunction (P=0.024). The final model demonstrated that renal dysfunction had a relative weighted impact of 3.2 versus CABG (1.7), age ≥60y (1.7) or NASH (1.3). Elevated LT+CS risk score was associated with an increased five-year mortality after surgery (AUC=0.731, P=<0.001). Conversely, the widely accepted STS-PROM calculator was unable to successfully stratify patients according to 1- (P>0.99) or 5-year (P=0.695) survival rates.

Conclusions:
This is the largest series describing combined LT+CS, with joint surgical management appearing feasible in highly selected patients. CABG and pre-operative renal dysfunction are important negative predictors of mortality. The four-variable LT+CS score may help predict patients at high risk for post-operative mortality.
Evaluating Clinical Presentation and Long-Term Outcomes in Individuals with Genetic and Non-Genetic Epilepsy Treated with Epilepsy Surgery: A Single-Center Study
Authors: Ivaniuk Alina , Boßelmann Christian M , Pestana-Knight Elia , Zhang Xiaoming , Jehi Lara , Bingaman William , Najm Imad , Lal Dennis 
Published: December 2023

AbstractBackgroundMany genetic epilepsy disorders are characterized by focal epilepsy or exhibit focal or lateralized features, which make individuals with such conditions potential surgical candidates. However, surgical outcomes in epilepsy caused by germline genetic variants and value of genetic testing in presurgical assessment remains unclear.MethodsThis retrospective cross-sectional study included people with germline genetic epilepsy and non-genetic epilepsy identified among 2879 people with epilepsy who underwent resective surgery or laser ablation at Cleveland Clinic in years 1997-2022. We separated individuals with germline genetic epilepsies into mTORopathies (clinically or genetically diagnosed tuberous sclerosis and genetically diagnosed GATOR-related epilepsies) and other genetic etiologies. We compared clinical variables between the groups using Kruskal-Wallis and Fisher tests as appropriate. We analyzed cumulative seizure recurrence risk over 5 years with Kaplan-Meier curves and used Cox proportional hazards model for univariate and multivariate analysis. We repeated the analysis after matching both genetic sub-groups to non-genetic group 1:1 by propensity scores estimated by a generalized linear model.ResultsWe included 49 individuals with genetic epilepsies (32 individuals with mTORopathies and 17 individuals with other genetic etiologies) and 585 individuals with non-genetic epilepsy. Individuals with genetic epilepsies in both groups were younger at seizure onset (p<0.001) and at surgery (p<0.001), had a higher preoperative seizure frequency (p<0.001), higher rate of developmental delay (p<0.001), lower rate of focal interictal (p=0.005) and ictal (p<0.001) findings on EEG, and more extratemporal and extensive resections (p<0.001). At the last follow-up, individuals with other genetic etiologies had a lower rate of seizure freedom than individuals with mTORopathies and non-genetic etiologies; still, 70.6% achieved seizure reduction. Individuals with other genetic etiologies had a higher cumulative risk of seizure recurrence at 5 years both in univariate (HR 3.07, 95% CI 1.4-6.74) and multivariate (HR 3.81, 95% CI 1.69-8.62, p=0.001) analyses, which was reproducible in a matched cohort analysis.ConclusionsPeople with germline genetic epilepsy across multiple etiologies could be surgical candidates. However, seizure recurrence may be faster in genetic epilepsies other than mTORopathies. Further studies are required to clarify surgical candidacy in different genetic epilepsy disorders and establish value of presurgical genetic testing.
Chloroplast genome-based genetic resources for Japan’s threatened subalpine forests via genome skimming
Authors: Worth James R.P. , Kikuchi Satoshi , Kanetani Seiichi , Takahashi Daiki , Aizawa Mineaki , Marchuk Elena A. , Choi Hyeok Jae , Polezhaeva Maria A. , Sheiko Viktor V. , Ueno Saneyoshi 
Published: December 2023

AbstractThe Japanese subalpine zone is dominated by a distinct and ecologically important conifer rich forest biome, subalpine coniferous forests, that are an outlier of the extensive boreal forests of Eurasia. While being relatively intact compared to other forest biomes in Japan, subalpine coniferous forests are under significant threat from deer browsing, global warming and small populations size effects. However, there is a severe lack of genetic resources available for the study of this biome’s major constituent plant species. This study aims to develop chloroplast genome-based genetic resources for 12 widespread subalpine tree and shrub species via genome skimming of whole genomic DNA using short reads (100-150 bp in length). For 10 species, whole chloroplast genomes were assembled viade novo-based methods from 4-10 individuals per species sampled from across their ranges in Japan and, for non-Japanese endemic species, elsewhere in northeast Asia. A total of 566 single nucleotide polymorphisms for Japanese samples and 768 for all samples (varying from 2 to 202 per species) were identified which were distributed in geographically restricted lineages in most species. In addition, between 9 to 58 polymorphic simple sequence repeat regions were identified per species. For two Ericaceae species (Rhododendron brachycarpumandVaccinium vitis-idaea) characterized by large chloroplast genomes,de novoassembly failed, but single nucleotide polymorphisms could be identified using reference mapping. This data will be useful for genetic studies of the taxonomic relationship of populations within Japan and to other parts of northeast Asia, investigating phylogeographic patterns within species, conservation genetics and has potential application for studies of environmental and ancient DNA.
Yeast9: A Consensus Yeast Metabolic Model Enables Quantitative Analysis of Cellular Metabolism By Incorporating Big Data
Authors: Zhang Chengyu , Sánchez Benjamín J. , Li Feiran , Eiden Cheng Wei Quan , Scott William T. , Liebal Ulf W. , Blank Lars M. , Mengers Hendrik G. , Anton Mihail , Rangel Albert Tafur , Mendoza Sebastián N. , Zhang Lixin , Nielsen Jens , Lu Hongzhong , Kerkhoven Eduard J. 
Published: December 2023

AbstractGenome-scale metabolic models (GEMs) can facilitate metabolism-focused multi-omics integrative analysis. Since Yeast8, the yeast-GEM ofSaccharomyces cerevisiae, published in 2019, has been continuously updated by the community. This have increased the quality and scope of this model, culminating now in Yeast9. To evaluate its predictive performance, we generated 163 condition-specific GEMs constrained by single-cell transcriptomics from osmotic pressure or normal conditions. Comparative flux analysis showed that yeast adapting to high osmotic pressure benefits from upregulating fluxes through the central carbon metabolism. Furthermore, combining Yeast9 with proteomics revealed metabolic rewiring underlying its preference in nitrogen sources. Lastly, we created strain-specific GEMs (ssGEMs) constrained by transcriptomics for 1229 mutant strains. Well able to predict the strains’ growth rates, fluxomics from those large-scale ssGEMs outperformed transcriptomics in predicting functional categories for all studied genes in machine-learning models. Based on those findings we anticipate that Yeast9 will empower systems biology studies of yeast metabolism.
Synthetic Lethality Screening with Recursive Feature Machines
Authors: Cai Cathy , Radhakrishnan Adityanarayanan , Uhler Caroline 
Published: December 2023

AbstractSynthetic lethality refers to a genetic interaction where the simultaneous perturbation of gene pairs leads to cell death. Synthetically lethal gene pairs (SL pairs) provide a potential avenue for selectively targeting cancer cells based on genetic vulnerabilities. The rise of large-scale gene perturbation screens such as the Cancer Dependency Map (DepMap) offers the opportunity to identify SL pairs automatically using machine learning. We build on a recently developed class of feature learning kernel machines known as Recursive Feature Machines (RFMs) to develop a pipeline for identifying SL pairs based on CRISPR viability data from DepMap. In particular, we first train RFMs to predict viability scores for a given CRISPR gene knockout from cell line embeddings consisting of gene expression and mutation features. After training, RFMs use a statistical operator known as average gradient outer product to provide weights for each feature indicating the importance of each feature in predicting cellular viability. We subsequently apply correlation-based filters to re-weight RFM feature importances and identify those features that are most indicative of low cellular viability. Our resulting pipeline is computationally efficient, taking under 3 minutes for analyzing all 17, 453 knockouts from DepMap for candidate SL pairs. We show that our pipeline more accurately recovers experimentally verified SL pairs than prior approaches. Moreover, our pipeline finds new candidate SL pairs, thereby opening novel avenues for identifying genetic vulnerabilities in cancer.
The dual coding of a single sex pheromone receptor in regulating the mating behavior of Asian HoneybeeApis cerana
Authors: Ke Haoqin , Bohbot Jonathan D. , Chi Yongjuan , Duan Shiwen , Ma Xiaomei , Ren Bingzhong , Wang Yinliang 
Published: December 2023

AbstractIn honeybee society, a virgin queen usually mates only once with several drones before founding a colony. For the rest of her prolific life, she will not engage in subsequent mating events. In the Asian honeybeeApis cerana, the mechanisms controlling this reproductive strategy involves the queen-released mandibular pheromone (QMP). This pheromone blend regulates the physiology and reproductive behavior of workers and drones. Its main component, 9-oxo-(E)-2-decenoic acid (9-ODA), acts as a sex pheromone and attracts drones. However, how the QMP prevents additional mating later in the queen’s life remains elusive. Using behavioral and chemical analysis, we show that the QMP component methylp-hydroxybenzoate (HOB) released by mated queens inhibits male attraction to 9-ODA. Furthermore,in vivoelectroantennogram and single sensillum recording data indicated that HOB alone significantly reduces the spontaneous spike activity of 9-ODA-sensitive olfactory receptor neurons (ORNs). To explore the molecular mechanism underlying this inverse effect, we conducted qPCR and in situ hybridization assays. The results indicated thatAcerOr11is specifically expressed in sensilla placodea from the drone’s antennae, which are the sensilla that narrowly respond to both 9-ODA and HOB. We then cloned and expressedAcerOr11in a Xenopus oocyte expression system, whereAcerOr11induced robust inward (regular) currents in response to 9-ODA. Intriguingly, HOB induced inverse currents in a dose-dependent manner. This suggests that HOB may act as an inverse agonist againstAcerOr11, providing additional odor-coding information. Based on these findings, we propose a model in whichAcerOr11function as a dual modulators in regulating the mating behavior ofA. cerana. The inverse agonist, HOB, can help manage the population dynamics of honeybees in apiculture.
Adjuvant nivolumab, capecitabine or the combination in patients with residual triple-negative breast cancer: the OXEL randomized phase II study
Authors: Lynce Filipa , Mainor Candace , Donahue Renee N. , Geng Xue , Jones Greg , Schlam Ilana , Wang Hongkun , Toney Nicole J. , Jochems Caroline , Schlom Jeffrey , Zeck Jay , Gallagher Christopher , Nanda Rita , Graham Deena , Stringer-Reasor Erica M , Denduluri Neelima , Collins Julie , Chitalia Ami , Tiwari Shruti , Nunes Raquel , Kaltman Rebecca , Khoury Katia , Gatti-Mays Margaret , Tarantino Paolo , Tolaney Sara M. , Swain Sandra M , Pohlmann Paula , Parsons Heather A. , Isaacs Claudine 
Published: December 2023

AbstractChemotherapy and immune checkpoint inhibitors have a role in the post-neoadjuvant setting in patients with triple-negative breast cancer (TNBC). However, the effects of nivolumab, a checkpoint inhibitor, capecitabine, or the combination in changing peripheral immunoscore (PIS) remains unclear. This open-label randomized phase II OXEL study (NCT03487666) aimed to assess the immunologic effects of nivolumab, capecitabine, or the combination in terms of the change in PIS (primary endpoint). Secondary endpoints include the presence of ctDNA, toxicity, clinical outcomes at 2-years and association of ctDNA and PIS with clinical outcomes. Forty-five women with TNBC and residual invasive disease after standard neoadjuvant chemotherapy were randomized to nivolumab, capecitabine, or the combination. Here we show that a combination of nivolumab plus capecitabine leads to a greater increase in PIS from baseline to week 6 (91%) compared with nivolumab (47%) or capecitabine (53%) alone (log-rank p = 0.08), meeting the pre-specified primary endpoint. In addition, the presence of circulating tumor DNA (ctDNA) was associated with disease recurrence, with no new safety signals in the combination arm. Our results provide efficacy and safety data on this combination in TNBC and support further development of PIS and ctDNA analyses to identify patients at high risk of recurrence.
Performance of ICD-10-based injury severity scores in pediatric trauma patients using the ICD-AIS map and survival rate ratios
Authors: Hojeij Rayan , Brensing Pia , Nonnemacher Michael , Kowall Bernd , Felderhoff-Müser Ursula , Dudda Marcel , Dohna-Schwake Christian , Stang Andreas , Bruns Nora 
Published: December 2023

AbstractObjectiveOur study aimed to identify the superior predictor of mortality from International Classification of Diseases 10 (ICD-10) codes among pediatric trauma patients in the German hospital database (GHD), a nationwide database comprising all hospitalizations in the country.Study design and settingHospital admissions of patients aged < 18 years with injury-related ICD-10 codes were selected. The maximum abbreviated injury scale (MAIS) and injury severity score (ISS) were calculated using the ICD-AIS map provided by the Association for the Advancement of Automotive Medicine, which we adjusted to the German modification of the ICD-10 classification. The survival risk ratio was used to calculate the single worst ICD-derived injury (single ICISS) and a multiplicative injury severity score (multiplicative ICISS). The ability to predict mortality of the four above mentioned scores were compared in the selected trauma population and within four clinically relevant subgroups using discrimination and calibration.ResultsOut of 13,992,596 cases < 18 years of age hospitalized between 2014 and 2020, 1,720,802 were trauma patients and ICD-AIS mapping was possible in 1,328,377 cases. Mortality was highest in patients with only one coded injury. Cases with mapping failure (n = 392,425; 22.8 %) were younger and had a higher mortality rate. SRR-derived scores had a better discrimination calibration than ICD-AIS based scores in the overall cohort and all four subgroups (AUC ranges between 0.985 and 0.998 versus 0.886 and 0.972 respectively).ConclusionEmpirically derived measures of injury severity were superior to ICD-AIS mapped scores in the GHD to predict mortality in pediatric trauma patients. Given the high percentage of mapping failure and high mortality among cases with single coded injury, the single ICISS may be the most suitable measure of injury severity in this group of patients.