bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-4270 suppresses hepatocellular carcinoma progression by inhibiting DNMT3A-mediated methylation of HGFAC promoter
Authors: Zou Qiang , Cao Shasha 
Published: December 2023

Background
miR-4270 is a regulatory factor has been linked with the progression of various cancers, such as nasopharyngeal carcinoma, hepatocellular carcinoma (HCC), and gastric cancer. However, the underlying mechanisms through which miR-4270 modulates HCC development are not fully understood.

Methods
miR-4270 expression levels were analyzed in various HCC cell lines and tissue samples. An online bioinformatics tool was then utilized to predict the miR-4270 target gene. The binding relationship between miR-4270 and its target gene DNMT3A was verified using dual-luciferase reporter and Ago2–RIP assays. Then, co-immunoprecipitation (Co-IP) and chromatin immunoprecipitation (ChIP) assays were conducted to investigate the association between DNMT3A and the hepatocyte growth factor activator (HGFAC) promoter region. To assess the methylation level of the HGFAC promoter, methylation-specific PCR (MSP) was employed. Furthermore, rescue analyses were carried out to evaluate the functional relevance of miR-4270 and HGFAC in the modulation of the malignant properties of HCC cells. Finally, HepG2 cells overexpressing miR-4270 were subcutaneously injected into nude mice to estimate the impact of miR-4270 on the xenograft tumor growth of HCC.

Results
A substantial miR-4270 downregulation was revealed in HCC patient samples and cell lines. miR-4270 upregulation suppressed both cell proliferation and invasion while promoting apoptosis. At the molecular level, miR-4270 was found to bind to the 3′untranslated region (3′UTR) of DNMT3A, thereby inhibiting DNMT3A-mediated methylation of the HGFAC promoter. Functional assays indicated that inhibition of miR-4270 stimulated HCC cell growth, an effect counteracted by overexpression of HGFAC. In vivo assays further verified that miR-4270 effectively suppressed the progression of HCC xenograft tumors.

Conclusions
miR-4270 was found to mitigate the malignant characteristics of HCC by inhibiting DNMT3A-mediated methylation of the HGFAC promoter, suggesting a potential therapeutic avenue for the management of HCC.
Severe Malaria and Risk Factors for Death in the Infectious Diseases Department of the University Hospital of Fann in Dakar, Senegal
Authors: Cisse Viviane Marie Pierre , Mbaye Khardiata Diallo , Badiane Aboubakar Sidikh , Diallo Malado , Diop Moustapha , Lakhe Ndèye Aïssatou , Fall Ndèye Maguette , Diouf Assane , Massaly Aminata , Ka Daye , Fortes Louise , Diop Sylvie Audrey , Seydi Moussa 
Published: December 2023

Aim: The aim of this study was to describe the epidemiological, clinical, paraclinical and outcome aspects of severe malaria and to determine the risk factors for death.
Methodology: This was a retrospective descriptive and analytical study of patients treated for severe malaria at the infectious and tropical diseases department of the University Hospital center of Fann between January 1, 2019 and December 31, 2021. Multivariate logistic regression was used to identify risk factors for death
Results: During our study, 138 patients were hospitalized for severe malaria over a period of 3 years, with a peak in the months of october and november. They were mostly from urban areas (68.11%). The average consultation time was 6.7 days ± 7.1 days. There was a clear male predominance, with a sex ratio of 3.18. The average age of patients was 33.96 ± 17 years. The main signs of clinical severity were coma (44.2%), prostration (36.95%) and jaundice (61.5%). Biological signs included anaemia (14.49%) and renal failure (9.42%). Severe malaria was associated with SARS Cov 2 infection in 4 patients. All patients were treated with injectable artesunate. The average length of hospitalization was 6 ± 6 days. Case fatality was 10.7. In multivariate analysis, coma (ORa =12.29 IC95%: 2.0 - 73.22 P=0.006) and severe anaemia (ORa=27.16 IC95%: 3.4 - 217.18 P=0.006) were risk factors associated with death.
Conclusion: Early recognition of the signs of seriousness and their early management thanks to improved technical facilities in intensive care units can improve the prognosis of patients.
Organizing Community Participation in Humanitarian Blood Donation Activities
Authors: Diani Yusias Hikmat , Sunarti Lusia Sri , Suryowati Trini , Lumbantobing Romauli , Sidauruk Morado Natanael , Putra Jeremya Yogi , Tambunan Hidekel , Samosir Daniel Jhosias 
Published: December 2023

Aim: To organize and to encourage community participation in humanitarian blood donation held by the Indonesian Red Cross in collaboration with local church.
Discussion: The culture of voluntary blood donation must always be encourage, in order to guarantee timely access to safe blood and blood products and help save lives. Compliment and appreciation given to noble donation of voluntary donors who receive no remuneration but are always ready and available to donate blood to help save other lives most of whom they never meet. Using popular social media, action taken to promote, mobilize, and publish educational materials on voluntary blood donation campaigns as well as encourage local potential donor to donate voluntarily. This activity is also the implementation of the noble human values ​​of sharing, giving and caring
Conclusion: Connecting all stakeholders helps to make the blood donation succesfull.
Response of Crop Establishment Methods and Weed Management Practices on Weed Flora, Crop Growth and Yield of Wheat in Maize-Wheat-Greengram Cropping System
Authors: Kumar Narendra , Choudhary V. K. , Sasode D. S. , Sahu Muni Pratap , Singh Vikash , Gajbhiye Mrinali , Kumhare Alpana , Singh Sonali 
Published: December 2023

Sustainable agriculture mostly relies on conservation tillage, crop residue retention and crop diversification. Weeds are major production hurdles in adoption of conservation agriculture (CA), therefore, weed management is important for sustainable crop yield. Designing efficient integrated weed management (IWM) practices under zero-tillage with crop residue (ZT+R) is helpful in getting optimum yield. A field experiment was carried out at research farm of ICAR-Directorate of Weed Research, Jabalpur (M.P.), India with eight treatment combinations, two crop establishment methods (conventional tillage and conservation tillage) in main plots and four weed management practices (weedy check, recommended herbicide, IWM, and herbicide rotation) in sub-plots were evaluated using a split plot design with three replications. The results indicated that the wheat sown under ZT+R led to significantly less weed density (105.9 no. m-2 in 2020-21 and 122.8 no. m-2 in 2022-23) and biomass (149.7 and 174.2 g m-2, respectively) hence, registered the highest weed control efficiency (WCE, 72.3% and 71.2%, respectively). Higher WCE helped in producing better wheat growth indices like plant height (106.3 and 101.1 cm, respectively), biomass (12.0 and 9.0 g plant-1, respectively) and no. of tillers (488.0 and 391.3 no. m-2, respectively) ultimately grain yield (4164 and 3814 kg ha-1, respectively) and straw yield (7265 and 6834 kg ha-1, respectively) compared with conventional tillage during both the years. Among the weed management practices, ready-mix application of clodinafop + metsulfuron at 60+4 g ha-1 (30-35 days after sowing, DAS) followed by (fb) hand weeding at 45 DAS increased growth indices like plant height (110.2 and 105.0 cm, respectively), plant biomass (14.9 and 12.1 g plant-1, respectively) and no. of tillers (547.2 and 436.7 no. m-2, respectively) ultimately grain yield (4708 and 4299 kg ha-1, respectively) and straw yield (7836 and 7610 kg ha-1, respectively), and WCE (97.4% and 96.8% respectively) with significantly less weed density (25.7 and 35.0 no. m-2, respectively) and biomass (13.6 and 19.9 g m-2, respectively) at 90 days after sowing. IWM also completely reduces the weed density and biomass over the other weed management practices during both the years. Therefore, wheat sown under ZT+R coupled with IWM was superior in terms of weed control, crop growth and yields.
Reporting of Factorial Randomized Trials
Authors: Kahan Brennan C. , Hall Sophie S. , Beller Elaine M. , Birchenall Megan , Chan An-Wen , Elbourne Diana , Little Paul , Fletcher John , Golub Robert M. , Goulao Beatriz , Hopewell Sally , Islam Nazrul , Zwarenstein Merrick , Juszczak Edmund , Montgomery Alan A. 
Published: December 2023

ImportanceTransparent reporting of randomized trials is essential to facilitate critical appraisal and interpretation of results. Factorial trials, in which 2 or more interventions are assessed in the same set of participants, have unique methodological considerations. However, reporting of factorial trials is suboptimal.ObjectiveTo develop a consensus-based extension to the Consolidated Standards of Reporting Trials (CONSORT) 2010 Statement for factorial trials.DesignUsing the Enhancing the Quality and Transparency of Health Research (EQUATOR) methodological framework, the CONSORT extension for factorial trials was developed by (1) generating a list of reporting recommendations for factorial trials using a scoping review of methodological articles identified using a MEDLINE search (from inception to May 2019) and supplemented with relevant articles from the personal collections of the authors; (2) a 3-round Delphi survey between January and June 2022 to identify additional items and assess the importance of each item, completed by 104 panelists from 14 countries; and (3) a hybrid consensus meeting attended by 15 panelists to finalize the selection and wording of items for the checklist.FindingsThis CONSORT extension for factorial trials modifies 16 of the 37 items in the CONSORT 2010 checklist and adds 1 new item. The rationale for the importance of each item is provided. Key recommendations are (1) the reason for using a factorial design should be reported, including whether an interaction is hypothesized, (2) the treatment groups that form the main comparisons should be clearly identified, and (3) for each main comparison, the estimated interaction effect and its precision should be reported.Conclusions and RelevanceThis extension of the CONSORT 2010 Statement provides guidance on the reporting of factorial randomized trials and should facilitate greater understanding of and transparency in their reporting.
Objective Sleep Duration and All-Cause Mortality Among People With Obstructive Sleep Apnea
Authors: Lin Yiqi , Wu Yongxi , Lin Qianwen , Wing Yun Kwok , Xu Lili , Ge Junbo , Wu Qinwei , Li Zhen , Wu Qingjie , Lin Beiwei , Wei Shichao 
Published: December 2023

ImportanceThe association between sleep duration and all-cause mortality remains unclear among people with obstructive sleep apnea (OSA).ObjectiveTo explore whether there is an association between sleep duration and all-cause mortality among people with OSA.Design, Setting, and ParticipantsThis cohort study investigated participants with OSA from the Sleep Heart Health Study (SHHS) in which participants were enrolled between 1995 and 1998 with questionnaires and polysomnography (PSG) assessment and followed up for a median of 11.8 years. SHHS was a multicenter community-based study; 2574 participants with OSA defined by apnea-hypopnea index (AHI) greater than or equal to 15 from SHHS were found; all of them had all-cause mortality data and were included in the study. Data were analyzed from November 2022 to October 2023.ExposuresParticipants were divided into 4 groups with objective sleep duration of (1) at least 7 hours, (2) 6 to less than 7 hours, (3) 5 to less than 6 hours, and (4) less than 5 hours, which was determined by total sleep time on PSG at baseline.Main Outcomes and MeasuresAll-cause mortality was defined as deaths from any cause and its risk was compared among 4 OSA groups using Cox regression models.ResultsA total of 2574 participants with OSA were included (1628 [63.2%] men and 946 [36.8%] women; mean [SD] age, 65.4 [10.7] years; 211 [8.2%] Black, 2230 [86.6%] White, 133 [5.2%] other race). Overall, 688 all-cause deaths were observed in participants. Compared with the group sleeping at least 7 hours, the groups sleeping 6 to less than 7 hours (hazard ratio [HR], 1.53 [95% CI, 1.13-2.07]), 5 to less than 6 hours (HR, 1.40 [95% CI, 1.03-1.90]), and less than 5 hours (HR, 1.64 [95% CI, 1.20-2.24]) had significantly higher risks of all-cause mortality independent of AHI. Sensitivity analyses were performed among participants with available data of positive airway pressure treatment during follow-up and the finding was mostly consistent, albeit the HR for the group of 5 to less than 6 hours was not statistically significant.Conclusions and RelevanceIn this cohort study of 2574 participants with OSA, those with shorter objective sleep duration had higher risk of all-cause mortality independent of AHI compared with those sleeping at least 7 hours. Further studies would be needed to investigate health benefits of extending sleep length among people with OSA with short sleep duration.
Consensus Statement for Protocols of Factorial Randomized Trials
Authors: Kahan Brennan C. , Hall Sophie S. , Beller Elaine M. , Birchenall Megan , Elbourne Diana , Juszczak Edmund , Little Paul , Fletcher John , Golub Robert M. , Goulao Beatriz , Hopewell Sally , Islam Nazrul , Zwarenstein Merrick , Chan An-Wen , Montgomery Alan A. 
Published: December 2023

ImportanceTrial protocols outline a trial’s objectives as well as the methods (design, conduct, and analysis) that will be used to meet those objectives, and transparent reporting of trial protocols ensures objectives are clear and facilitates appraisal regarding the suitability of study methods. Factorial trials, in which 2 or more interventions are assessed in the same set of participants, have unique methodological considerations. However, no extension of the Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) 2013 Statement, which provides guidance on reporting of trial protocols, for factorial trials is available.ObjectiveTo develop a consensus-based extension to the SPIRIT 2013 Statement for factorial trials.Evidence ReviewThe SPIRIT extension for factorial trials was developed using the Enhancing the Quality and Transparency of Health Research (EQUATOR) methodological framework. First, a list of reporting recommendations was generated using a scoping review of methodological articles identified using a MEDLINE search (inception to May 2019), which was supplemented with relevant articles from the personal collections of the authors. Second, a 3-round Delphi survey (January to June 2022, completed by 104 panelists from 14 countries) was conducted to assess the importance of items and identify additional recommendations. Third, a hybrid consensus meeting was held, attended by 15 panelists to finalize selection and wording of the checklist.FindingsThis SPIRIT extension for factorial trials modified 9 of the 33 items in the SPIRIT 2013 checklist. Key reporting recommendations were that the rationale for using a factorial design should be provided, including whether an interaction is hypothesized; the treatment groups that will form the main comparisons should be identified; and statistical methods for each main comparison should be provided, including how interactions will be assessed.Conclusions and RelevanceIn this consensus statement, 9 factorial-specific items were provided that should be addressed in all protocols of factorial trials to increase the trial’s utility and transparency.
Hydrophobic Trinuclear Copper Cluster‐Containing Organic Framework for Synergetic Electrocatalytic Synthesis of Amino Acids
Authors: Li Ning , Pan Chenliang , Lu Guang , Pan Houhe , Han Yuesheng , Wang Kang , Jin Peng , Liu Qingyun , Jiang Jianzhuang 
Published: December 2023

AbstractElectrocatalytic synthesis of amino acids provides a promising green and efficient pathway to manufacture the basic substances of life. Herein, reaction of 2,5‐perfluroalkyl‐terepthalohydrazide and tris(4‐µ2‐O‐carboxaldehyde‐pyrazolato‐N, N′)‐tricopper affords a crystalline trinuclear copper cluster‐containing organic framework, named F‐Cu3‐OF. Incorporation of abundant hydrophobic perfluroalkyl groups inside the channels of F‐Cu3‐OF is revealed to successfully suppress the hydrogen evolution reaction via preventing H+ cation with large polarity from the framework of F‐Cu3‐OF and in turn increasing the adsorption of other substrates with relatively small polarity like NO3− and keto acids on the active sites. The copper atoms with short distance in the trinuclear copper clusters of F‐Cu3‐OF enable simultaneous activization of NO3− and keto acids, facilitating the following synergistic and efficient C─N coupling on the basis of in situ spectroscopic investigations together with theoretical calculation. Combination of these effects leads to efficient electroproduction of various amino acids including glycine, alanine, leucine, valine, and phenylalanine from NO3− and keto acids with a Faraday efficiency of 42%–71% and a yield of 187–957 µmol cm−2 h−1, representing the thus far best performance. This work shall be helpful for developing economical, eco‐friendly, and high‐efficiency strategy for the production of amino acids and other life substances.
In‐Depth Compositional Analysis of the Carbon‐Rich Fine‐Grain Layer in Solution‐Processed CZTSSe Films Accessed by a Photonic Lift‐Off Process
Authors: Javed Ahmed , Jones Michael , Campbell Stephen , Yerci Selcuk , Barrioz Vincent , Qu Yongtao 
Published: December 2023

AbstractThe existence of a fine‐grain (FG) sub‐layer between the top large‐grain (LG) layer and the back contact is widely observed in kesterite absorbers prepared with organic solvents. In this paper, the distinguishing features of the lifted‐off carbon‐rich FG layer are investigated through direct analysis with a series of characterization techniques, including X‐ray photoelectron spectroscopy (XPS), attenuated total reflectance, X‐ray diffraction, and scanning electron microscopy. To access the FG layer for direct probing, a scalable and repeatable photonic lift‐off method is developed for carrying out the separation of the kesterite absorber layer from the Mo‐coated glass substrate. A very high light intensity of 4 kW cm−2for a short interval of 1 ms is optimized by COMSOL simulations, and successful implementation is demonstrated. The XPS analysis has revealed significant carbon content at the exposed FG surface, which explains the hindrance of grain growth due to carbon abundance. The variations in cations and anions concentrations from FG layer leading into LG region are explored through argon ions (Ar+) assisted XPS depth profiling. The observed significant differences between the composition of FG and LG regions are speculated to negatively impact the performance of solar cells.
Determinants of municipal‐level household food waste
Authors: Deng Wen‐Shuenn , Lin Yi‐Chen 
Published: December 2023

AbstractThis research investigates the long‐run determinants of per capita food waste, using a monthly time series dataset collected from the Taipei City Food Scraps Recycling Program, covering a span of 19 years. In addition, this research introduces a new proxy for the food wastage rate, gauged by the ratio of edible‐to‐inedible food scraps. The results of time‐series cointegration analysis show that per capita edible food scraps and socioeconomic factors are bound together over the long run. Specifically, per capita edible food waste displays a positive co‐movement with food price, the working‐age share, and household size, while exhibiting a negative co‐movement with the old‐age share. This insight empowers policymakers with the ability to foresee and tackle the food waste problem by acting on the municipality's demographic and economic structural change. Our analysis complements the existing studies that depend on cross‐sectional individual‐level data by adopting a long‐run municipal‐level time series perspective. [EconLit Citations: Q11, Q13, Q18, D1].
Development and assessment of a stair ascension challenge as a measure of aging and physical function in nonhuman primates
Authors: Scarberry Shannon R. , Prutchi Hannah , Frye Brett M. , Herr Justin , Scott Christie , Long Chrissy M. , Jorgensen Matthew J. , Shively Carol A. , Kavanagh Kylie 
Published: December 2023

AbstractNonhuman primates (NHPs) are valuable models for studying healthspan, including frailty development. Frailty metrics in people centers on functional measures, including usual gait speed which can be predictive of all‐cause mortality. This concept that physical competencies are able to prognosticate an individual's health trajectory over chronologic aging is well‐accepted and has led to refinements in how physical function is evaluated, and include measures of strength and power along with walking speed. NHP studies of aging require evaluation of physical function, which can be difficult in field and research settings. We compared stair climb velocity to usual walking speed in 28 peri‐geriatric to geriatric NHPs, as incorporating a climbing obstacle integrates multiple components of physical function: isolated leg and back strength, proprioception, balance, and range of motion. We find that stair climbing speed was reliable between observers, and whether timing was in‐person take from video capture. The stair climb rates were 50% more associated with chronological age than walking speed (R = −0.68 vs. −0.45) and only stair climbing speeds were retained as predictive of age when walking speed and bodyweight were included in multivariate models (overall R2 = 0.44; p < 0.0001). When comparing young (10−16 years) versus geriatric (16−29 years) stair climbing speed was significantly different (p < 0.001), while walking speeds only tended to be slower (p = 0.12) suggesting that the additional challenge of a stair climb better unmasks subclinical frailty development that usual walking speed.
Porous Single‐crystalline Centimeter‐sized α‐Al2O3Monoliths for Selective and Durable Non‐oxidative Dehydrogenation of Ethane
Authors: Li Xue , Li Wenting , Zhang Jie , Yin Wen , Xia Yuanguang , Xie Kui 
Published: December 2023

AbstractAlpha alumina (α‐Al2O3) are inert materials with outstanding thermal, chemical and mechanical stability. Herein, we fabricate porous single‐crystalline (PSC) α‐Al2O3monoliths at centimeter scale to endow them with high catalytic activity while maintaining their stability. We reduce PSC α‐Al2O3monoliths to create oxygen vacancies in lattice and stabilize them by the ordered lattice to construct unsaturated Al−O coordination structures for enhancing the catalytic activity. The generation of oxygen vacancy at 18e wyckoff position contributes to the unsaturated Al−O coordination. As a case study, we demonstrate the outstanding performance with conversion (≈34 %) and selectivity (≈95 %) toward non‐oxidative dehydrogenation of ethane to ethylene at 700 °C. We achieve the outstanding performance without obvious degradation even after a continuous operation over 1000 hours at 700 °C.