bluuebunny/crossref_metadata_2025_split huggingface dataset:
LUTAS CORPORAIS NA PESPERCTIVA DE INCLUSÃO: POSSIBILIDADES PARA UM ALUNO CADEIRANTE
Authors: Felipe Dionny , Junior da Silva Daniel , Pereira Pires Flávio , Gonçalves de Abreu José Roberto , Do Nascimento Megal Lucas , Reis Lopes Thiago 
Published: October 2023

A presente pesquisa, possui como principal objetivo, propor uma sequência didático-pedagógico para o ensino das Lutas Corporais na perspectiva da inclusão para um aluno cadeirante, propondo assim, reflexões sobre a inclusão de um estudante cadeirante nas aulas de Educação Física no âmbito escolar. Desse modo, foi utilizado de pano de fundo para a construção do presente artigo, o documento norteador da rede pública municipal de São Mateus-ES, bem como autores que dialogam com o tema proposto. A metodologia adotada foi a de pesquisa de campo, sendo planejada 7 aulas que seguem uma progressão pedagógica por meio de atividades lúdicas. Conclui-se que, as Lutas Corporais na perspectiva da inclusão pode ser um relevante tema para a Educação Física Escolar, tendo em vista as diversas possibilidades para a contribuição com a formação integral do aluno enquanto sujeito participativo e crítico na sociedade. Além disso, espera-se que o trabalho em tela possa auxiliar futuros estudos nas áreas relacionadas a pesquisa.
Análisis de la Gestión de Riesgos: Caso de una empresa mediana del sector comercial en Guadalajara
Authors: Aguilar Pérez Pedro , Cruz Covarrubias Lucila Patricia 
Published: October 2023

El objetivo de esta investigación fue analizar el panorama de la gestión de riesgos en una empresa mediana de la ciudad de Guadalajara y evidenciar el conocimiento y la aplicación de la gestión de riesgos empresariales. El método de estudio asume un enfoque cualitativo con un procedimiento de estudio de caso, también se empleó la entrevista semiestructurada aplicada a cinco directivos de la organización objeto de estudio; asimismo, se entrevistaron a dos expertos externos en el área de riesgos. Los resultados muestran que la empresa realiza acciones de control interno y de cumplimiento legal para el logro de sus objetivos; sin embargo, manifiestan desconocer una metodología como la ISO 31000; y existe confusión entre concepto de riesgos y control interno, hecho que se demuestra en la concentración de gestión en algunas áreas y con escasa cultura enfocada a riesgos. En conclusión, la empresa realiza prácticas de mejora administrativa, permitiendo corregir algunas operaciones e incurren en no desarrollar competencias significativas para mitigar los riegos de la compañía.Palabras Clave: gestión de riesgos, mejora de procesos, prácticas empresariales, riesgos.
Abstracthe objective of the research was to analyze the panorama of risk management in a medium-sized company in the city of Guadala- jara. Likewise, it is intended to demonstrate the knowledge and application of business risk management. The study method assumes a qualitative approach, with a case study procedure, a semi-structured interview was used, conducted with five managers of the organization under study, and two external experts in the risk area were also inter- viewed. The results show that the company carries out internal control and legal compliance actions to achieve its objectives; they state that they are unaware of a methodology such as ISO 31000; there is con- fusion between the concept of risks and internal control, a fact that is demonstrated in the concentration of management in some areas and with little culture focused on risks. Conclusion: the company carries out administrative improvement practices allowing to correct some operations; they incur in not developing significant competences to mitigate the company’s risks.
Keywords: risk management, process improvement, business practices, risks
Computer controlled expansion of equine cord blood mesenchymal stromal cells on microcarriers in 3 L vertical-wheel® bioreactors
Authors: Roberts E. L. , Abraham B. D. , Dang T. , Gysel E. , Mehrpouyan S. , Alizadeh A. H. , Koch T. G. , Kallos M. S. 
Published: October 2023

Mesenchymal stromal cells (MSCs) are an ideal cell source for allogenic cell therapy due to their immunomodulatory and differentiation properties. Equine MSCs (eMSCs) have been found to be a promising treatment for equine joint injuries including meniscal injuries, cartilage degradation, and osteoarthritis. Although the use of eMSCs has shown efficacy in preliminary studies, challenges associated with biomanufacturing remain. To achieve the required cell numbers for clinical application, bioreactor-based processes are required. Initial studies have shown that eMSCs can be cultivated in microcarrier-based, stirred suspension bioreactor culture at the laboratory 0.1 L scale using a Vertical-Wheel® (VW) bioreactor. However, investigations regarding scale up of these processes to the required biomanufacturing scales are required. This study investigated the scale-up of a equine cord blood MSC (eCB-MSC) bioprocess in VW bioreactors at three scales. This included scale-up from the 0.1–0.5 L bioreactor, scale-up from static culture to the 3 L computer-controlled bioreactor, and scale-up into the 3 L computer-controlled bioreactor using a mock clinical trial process. Results from the various scale-up experiments demonstrated similar cell expansion at the various tested scales. The 3 L computer-controlled system resulted in a final cell densities of 1.5 × 105 cells/cm2 on average, achieving 1.5 × 109 harvested cells. Biological testing of the cells showed that cell phenotype and functionality were maintained after scale-up. These findings demonstrate the scalability of an eCB-MSC bioprocess using microcarriers in VW bioreactors to achieve clinically relevant cell numbers, a critical step to translate MSC treatments from research to clinical applications. This study also represents the first known published study expanding any cell type in the 3 L VW bioreactor.
Differential responses to maternal diabetes in embryo and visceral yolk sac
Authors: Salbaum J. Michael , Stone Kirsten P. , Kruger Claudia , Kappen Claudia 
Published: October 2023

Introduction: Maternal diabetes during pregnancy is well known to be associated with a higher risk for structural birth defects in the offspring. Recent searches for underlying mechanisms have largely focused on aberrant processes in the embryo itself, although prior research in rodent models implicated dysfunction also of the visceral yolk sac. The objective of our research was to investigate both tissues within the conceptus simultaneously.Methods: We conducted unbiased transcriptome profiling by RNA sequencing on pairs of individual yolk sacs and their cognate embryos, using the non-obese diabetic (NOD) mouse model. The analysis was performed at gestational day 8.5 on morphologically normal specimen to circumvent confounding by defective development.Results: Even with large sample numbers (n = 33 in each group), we observed considerable variability of gene expression, primarily driven by exposure to maternal diabetes, and secondarily by developmental stage of the embryo. Only a moderate number of genes changed expression in the yolk sac, while in the embryo, the exposure distinctly influenced the relationship of gene expression levels to developmental progression, revealing a possible role for altered cell cycle regulation in the response. Also affected in embryos under diabetic conditions were genes involved in cholesterol biosynthesis and NAD metabolism pathways.Discussion: Exposure to maternal diabetes during gastrulation changes transcriptomic profiles in embryos to a substantially greater effect than in the corresponding yolk sacs, indicating that despite yolk sac being of embryonic origin, different mechanisms control transcriptional activity in these tissues. The effects of maternal diabetes on expression of many genes that are correlated with developmental progression (i.e. somite stage) highlight the importance of considering developmental maturity in the interpretation of transcriptomic data. Our analyses identified cholesterol biosynthesis and NAD metabolism as novel pathways not previously implicated in diabetic pregnancies. Both NAD and cholesterol availability affect a wide variety of cellular signaling processes, and can be modulated by diet, implying that prevention of adverse outcomes from diabetic pregnancies may require broad interventions, particularly in the early stages of pregnancy.
Development of a depot formulation with an in situ non-lamellar liquid crystal-forming system with phospholipids
Authors: Todo Hiroaki , Niki Rina , Okada Akie , Narita Ibuki , Inamura Kazuya , Ito Ayu , Itakura Shoko , Hijikuro Ichiro , Sugibayashi Kenji 
Published: October 2023

Non-lamellar liquid crystal (NLLC) structures have gained increasing attention for the controlled release of entrapped drugs. In the present study, an in situ NLLC structure-forming depot formulation through contact with water was developed using a ternary mixture system of soya phosphatidyl choline (SPC), 1, 2-dioleoyl-sn-glycero-3-phosphoglycerol sodium salt (DOPG), and sorbitan trioleate (Span 85), and the long-term release of an entrapped model drug, leuprolide acetate (LA), was investigated using evaluation of in vitro release and in vivo blood concentration–time profiles. Polarized images and small angle X-ray scattering analysis were used to confirm the presence of NLLC structures by contacting the prepared formulation with water. In addition, LA release and blood concentration–time profiles were investigated using in vitro and in vivo experiments, respectively. In situ NLLC constructed formulations by contacting water were achieved using a ternary mixture of SPC, DOPG, and Span 85. In particular, negative curvature was increased with an increase in the amount of Span 85 in the formulation, and an Fd3m structure was obtained with a sustained release of LA. A maintained blood concentration of LA over 21 days was confirmed by subcutaneous (s.c.) administration of the formulation. No retained administered formulation at the injection site was confirmed 28 days after administration without any signs of irritation, inflammation, or other apparent toxicity confirmed by visual observation. This result may be helpful for the development of a lipid-based formulation of peptides and proteins with sustained drug release.
Worry effect for COVID-19 transmission over depression, anxiety, and satisfaction with studies in Peruvian university students
Authors: Mamani-Benito Oscar , Carranza Esteban Renzo Felipe , Castillo-Blanco Ronald , Lingán-Huamán Susana K. , Gálvez-Díaz Norma del Carmen , Huancahuire-Vega Salomón , Corrales-Reyes Ibraín Enrique 
Published: October 2023

IntroductionConcern about contracting COVID-19 has had repercussions on university students. The aim of this study was to determine its effect on depression, anxiety, and satisfaction with studies.MethodsUsing an explanatory cross-sectional design, 1,260 medical, nursing, and dentistry students from both public and private universities in Peru participated in this study. They were assessed using a scale for concern about contracting COVID-19, the Patient Health Questionnaire-2, the Generalized Anxiety Disorder-2 (GAD-2), and a brief scale for satisfaction with their studies.ResultsAfter analyzing the data using structural equation modeling, it was found that the explanatory model had an adequate fit. It showed that concern about COVID-19 had a direct and significant effect on anxiety (β = 0.46, p &lt; 0.001) and depression (β = 0.38, p &lt; 0.001). However, the same effect was not evident on satisfaction with studies (β = −0.06, p = 0.068).DiscussionThe uncertainty about contracting COVID-19 triggered symptoms of anxiety and depression among Peruvian university students. Still, it did not affect the satisfaction students felt when their academic expectations were met.
Features of the TCR repertoire associate with patients' clinical and molecular characteristics in acute myeloid leukemia
Authors: Pospiech Mateusz , Tamizharasan Mukund , Wei Yu-Chun , Kumar Advaith Maya Sanjeev , Lou Mimi , Milstein Joshua , Alachkar Houda 
Published: October 2023

BackgroundAllogeneic hematopoietic stem cell transplant remains the most effective strategy for patients with high-risk acute myeloid leukemia (AML). Leukemia-specific neoantigens presented by the major histocompatibility complexes (MHCs) are recognized by the T cell receptors (TCR) triggering the graft-versus-leukemia effect. A unique TCR signature is generated by a complex V(D)J rearrangement process to form TCR capable of binding to the peptide-MHC. The generated TCR repertoire undergoes dynamic changes with disease progression and treatment.MethodHere we applied two different computational tools (TRUST4 and MIXCR) to extract the TCR sequences from RNA-seq data from The Cancer Genome Atlas (TCGA) and examine the association between features of the TCR repertoire in adult patients with AML and their clinical and molecular characteristics.ResultsWe found that only ~30% of identified TCR CDR3s were shared by the two computational tools. Yet, patterns of TCR associations with patients’ clinical and molecular characteristics based on data obtained from either tool were similar. The numbers of unique TCR clones were highly correlated with patients’ white blood cell counts, bone marrow blast percentage, and peripheral blood blast percentage. Multivariable regressions of TCRA and TCRB median normalized number of unique clones with mutational status of AML patients using TRUST4 showed significant association of TCRA or TCRB with WT1 mutations, WBC count, %BM blast, and sex (adjusted in TCRB model). We observed a correlation between TCRA/B number of unique clones and the expression of T cells inhibitory signal genes (TIGIT, LAG3, CTLA-4) and foxp3, but not IL2RA, CD69 and TNFRSF9 suggestive of exhausted T cell phenotypes in AML.ConclusionBenchmarking of computational tools is needed to increase the accuracy of the identified clones. The utilization of RNA-seq data enables identification of highly abundant TCRs and correlating these clones with patients’ clinical and molecular characteristics. This study further supports the value of high-resolution TCR-Seq analyses to characterize the TCR repertoire in patients.
Anakinra authorized to treat severe coronavirus disease 2019; Sepsis breakthrough or time to reflect?
Authors: Shapiro Leland , Scherger Sias , Franco-Paredes Carlos , Gharamti Amal , Henao-Martinez Andrés F. 
Published: October 2023

IntroductionThe European Medicines Agency (EMA) and the United States Food and Drug Administration (FDA) announced conditions for using recombinant human interleukin-1 receptor antagonist (rhIL-1ra) to treat hospitalized patients with Coronavirus disease 2019 (COVID-19) and risk for progression. These decisions followed publication of the suPAR-guided Anakinra treatment for Validation of the risk and early Management OF seveRE respiratory failure by COVID-19 (SAVE- MORE) phase 3 clinical trial that yielded positive results.MethodsWe conducted a literature review and theoretical analysis of IL-1 blockade as a therapy to treat COVID-19. Using a stepwise analysis, we assessed clinical applicability of the SAVE-MORE results and evaluated conceptual support for interleukin-1 suppression as a suitable approach to COVID-19 treatment. This therapeutic approach was then examined as an example of inflammation-suppressing measures used to treat sepsis.ResultsAnakinra use as a COVID-19 therapy seems to rely on a view of pathogenesis that incorrectly reflects human disease. Since COVID-19 is an example of sepsis, COVID-19 benefit due to anti-inflammatory therapy contradicts an extensive history of unsuccessful clinical study. Repurposing rhIL-1ra to treat COVID-19 appears to exemplify a cycle followed by inflammation-suppressing sepsis treatments. A landscape of treatment failures is interrupted by a successful clinical trial. However, subsequent confirmatory study fails to replicate the positive data.DiscussionWe suggest further experimentation is not a promising pathway to discover game-changing sepsis therapies. A different kind of approach may be necessary.
The role of chicken eggs in modulating sarcopenic obesity and gut microbiota in db/db mice
Authors: Okamura Takuro , Hasegawa Yuka , Hamaguchi Masahide , Sasano Ryoichi , Fukui Michiaki 
Published: October 2023

BackgroundSarcopenia obesity, in which loss of muscle mass and fat accumulation occur simultaneously, is the pathological basis of type 2 diabetes mellitus. The usefulness of chicken eggs in sarcopenia prevention has been reported in several previous studies. The purpose of this study was to determine the beneficial effects of chicken eggs in the prevention of sarcopenic obesity in db/db mice.MethodsWe raised 8-week-old db/db male mice, a model of sarcopenia obesity, for 8 weeks and fed them a diet mixed with dried whole eggs. The fecal microbiota transplant (FMT) group was treated with antibiotics for 2 weeks, starting at 6 weeks of age, followed by FMT twice a week until 16 weeks of age.ResultsEggs administered to db/db mice showed increased grip strength (p = 0.022) and muscle mass (p = 0.013), decreased visceral fat mass (p = 0.005), and significantly increased physical activity (p &lt; 0.001). The FMT group of egg-fed mice showed a significant improvement in glucose intolerance and sarcopenic obesity. Sequencing of gene expression in the small intestine showed that the gene expression of amino acid transporters such as Slc6a18, Slc6a19, and Slc38a6 was increased in egg-fed mice. 16S rRNA sequencing of the gut microbiota showed an increase in the genus Vampirovibrio in both the egg-fed and FMT groups compared to that in egg-fed mice.ConclusionThe results of this study indicate that egg consumption not only increases the intake of amino acids and other nutrients but also alters the intestinal microbiota and increases amino acid absorption from the intestinal tract, suggesting that eggs might contribute to the ameliorative mechanism of sarcopenic obesity.
The causality between gut microbiome and anorexia nervosa: a Mendelian randomization analysis
Authors: Xia Xuan , He Shu-yang , Zhang Xiao-Lin , Wang Decheng , He Qian , Xiao Qing-Ao , Yang Yong 
Published: October 2023

Background and aimNutrient production by intestinal microbiota corresponds to regulate appetite while gut microbial composition was influenced by diet ingestion. However, the causal relationship between gut microbial taxa and anorexia nervosa (AN) remains unclear. Mendelian Randomization (MR) is a novel research method that effectively eliminates the interference of confounding factors and allows for the exploration of the direct causal effects between exposure and outcome. This study employs MR to explore the causal effect between AN and specific gut microbiome.MethodsLarge-scale Genome Wide Association Study (GWAS) data of AN and 211 gut microbes were obtained from the IEU open GWAS project and Mibiogen Consortium. Two-sample MR was performed to determine the causal relationship between gut microbiota and AN. Furthermore, a bi-directional MR analysis was to examine the direction of the causal relations. The Bonferroni correction test was used to adjust potential correlations among microbial taxa.ResultIn forward MR analysis, 10specific gut microbial taxa have an impact on the occurrence of AN (the p value of IVW &lt;0.05). The high abundance of Genus Eubacteriumnodatumgroup ID: 11297 (OR:0.78, 95% CI:0.62–0.98, p = 0.035) and Class Melainabacteria ID: 1589 (OR:0.72, 95% CI:0.51–0.99, p = 0.045) may be considered protective factors for AN. But after Bonferroni correction, only Class Actinobacteria ID:419 (OR:1.53, 95% CI:1.19–1.96, p = 0.00089) remained significantly associated and high abundance of Class Actinobacteria ID:419 considered as a risk factor for AN. In the reverse MR analysis, AN influences 8 gut microbial taxa with none-statistically significant associations after adjustment.ConclusionWe identified a significant correlation between AN and 18 microbial taxa which have not been previously reported. Among them, 10 kinds of gut bacteria may affect the occurrence of AN, and the status of AN would affect 8 kinds of gut bacteria. After correction, the Class Actinobacteria ID:419 continued to exert an influence on AN.
The value of computed tomography perfusion deficit volumes in acute isolated brainstem infarction
Authors: Chen Pengjun , Pan Yiying , Wang Jingke , Hui Junguo , Gao Ruijie , Lin Guihan , Li Bingrong , Rao Jie , Xia Shuiwei , Ji Jiansong 
Published: October 2023

PurposeDiagnosis of acute isolated brainstem infarction is challenging owing to non-specific, variable symptoms, and the effectiveness of non-contrast computed tomography (NCCT) is poor owing to limited spatial resolution and artifacts. Computed tomography perfusion (CTP) imaging parameters are significantly associated with functional outcomes in posterior circulation acute ischemic stroke; however, the role of CTP in isolated brainstem infarction remains unclear. We aimed to determine the value of CTP imaging parameters in predicting functional outcomes for affected patients.MethodsIn total, 116 consecutive patients with isolated pontine/midbrain hypoperfusion who underwent CTP and follow-up by magnetic resonance imaging (MRI) between January 2018 and March 2022, were retrospectively analyzed. Perfusion deficit volumes on all maps, and the final infarction volume (FIV) on MRI were quantified. “Good” functional outcome was defined as a 90-day modified Rankin Scale score of 0 and 1. Statistical analysis included uni- and multivariate regression analyses, binary logistic regressions, and receiver operating characteristics (ROC) analyses.ResultsIn total, 113 patients had confirmed isolated pontine/midbrain infarction on follow-up MRI. Onset-to-scan time, visibility of ischemic lesions on NCCT, the baseline National Institutes of Health Stroke Scale (NIHSS) score, and perfusion deficit volumes on all CTP maps were significantly associated with FIV (p &lt; 0.05). In a multivariate linear regression model, adjusted for age, sex, NIHSS score, onset-to-scan time, and visibility of NCCT, perfusion deficit volumes remained significantly associated with FIV. In binary logistic regression analyses, perfusion deficit volumes on all CTP maps remained independent predictors of a good functional outcome. In ROC analyses, the cerebral blood flow deficit volume showed a slightly higher discriminatory value with the largest area under the curve being 0.683 [(95% CI, 0.587–0.780), p = 0.001].ConclusionPerfusion deficit volumes of CTP imaging could reflect the FIV and contain prognostic information on functional outcomes in patients with acute isolated brainstem infarction.