bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rubisco in high Arctic tidewater glacier‐marine systems: A new window into phytoplankton dynamics
Authors: Roberts Megan E. , Bhatia Maya P. , Rowland Elden , White Patrick L. , Waterman Stephanie , Cavaco Maria A. , Williams Patrick , Young Jodi N. , Spence Jenifer S. , Tremblay Jean‐Éric , Montero‐Serrano Jean‐Carlos , Bertrand Erin M. 
Published: February 2024

AbstractThe hundreds of tidewater glaciers found in the Canadian Arctic Archipelago have the potential to enhance delivery of nutrients and other material to the surface ocean. Despite this, their influence on marine ecosystems, specifically phytoplankton, is poorly characterized. Here we developed and applied a quantitative mass spectrometry‐based approach to measure phytoplankton ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco) concentrations to examine differences in productivity in glacierized and non‐glacierized marine systems in Jones Sound, Nunavut, within Inuit Nunangat. Comparisons to chloroplast 16S rRNA gene amplicon sequencing data suggested that these measurements detect the majority of Rubisco produced in Jones Sound. Because Rubisco catalyzes carbon fixation, we used these measurements to estimate total and group‐specific primary production potential, which were within the range of historical primary production measurements made using classical methods in this region. Our measurements also revealed that up to 2% of total protein in the water column is Rubisco, and that Rubisco concentrations are correlated with chlorophyll fluorescence, with maxima near the nitracline. Rubisco produced by diatom genera Chaetoceros and Thalassiosira were higher in marine regions influenced by glaciers, while Rubisco from Micromonas (Chlorophyta) was greater in non‐glacierized regions. This suggests that future climate scenarios may favor smaller phytoplankton groups, like Micromonas, with consequences for food webs and carbon cycling. This study broadens our understanding of how tidewater glaciers will impact phytoplankton communities, now and in a warmer future, and lays the foundation for using this mass spectrometry‐based approach to quantify phytoplankton group‐specific carbon fixation potential in other marine regions.
Large‐Area, High‐Numerical‐Aperture, Freeform Metasurfaces
Authors: Zhou You , Mao Chenkai , Gershnabel Erez , Chen Mingkun , Fan Jonathan A. 
Published: February 2024

AbstractNanophotonic devices are optical platforms capable of unprecedented wavefront control. To push the limits of experimental device performance, scalable design methodologies that combine the simplicity and fabricability of conventional design paradigms with the extended capabilities of freeform optimization are required. A novel gradient‐based design framework for large‐area freeform metasurfaces is introduced in which nonlocal interactions between simply shaped nanostructures, placed on an irregular lattice, are tailored to produce high‐order hybridized modes that support customizable large‐angle scattering profiles. Utilizing this approach, multifunctional super‐dispersive metalenses are designed and experimentally demonstrated. The approach to high‐numerical‐aperture radial metalenses capable of diffraction limited focusing and the generation of donut‐shaped point spread functions is also extended. It is anticipated that these concepts will have utility in super‐resolution microscopy, particle trapping, additive manufacturing, and metrology applications that require ultra‐high numerical apertures.
Epigenetic regulation in cancer
Authors: Gu Minzhi , Ren Bo , Fang Yuan , Ren Jie , Liu Xiaohong , Wang Xing , Zhou Feihan , Xiao Ruiling , Luo Xiyuan , You Lei , Zhao Yupei 
Published: February 2024

AbstractEpigenetic modifications are defined as heritable changes in gene activity that do not involve changes in the underlying DNA sequence. The oncogenic process is driven by the accumulation of alterations that impact genome's structure and function. Genetic mutations, which directly disrupt the DNA sequence, are complemented by epigenetic modifications that modulate gene expression, thereby facilitating the acquisition of malignant characteristics. Principals among these epigenetic changes are shifts in DNA methylation and histone mark patterns, which promote tumor development and metastasis. Notably, the reversible nature of epigenetic alterations, as opposed to the permanence of genetic changes, positions the epigenetic machinery as a prime target in the discovery of novel therapeutics. Our review delves into the complexities of epigenetic regulation, exploring its profound effects on tumor initiation, metastatic behavior, metabolic pathways, and the tumor microenvironment. We place a particular emphasis on the dysregulation at each level of epigenetic modulation, including but not limited to, the aberrations in enzymes responsible for DNA methylation and histone modification, subunit loss or fusions in chromatin remodeling complexes, and the disturbances in higher‐order chromatin structure. Finally, we also evaluate therapeutic approaches that leverage the growing understanding of chromatin dysregulation, offering new avenues for cancer treatment.
Digital transformation, external financing, and enterprise resource allocation efficiency
Authors: Liu Yan , He Qiuju 
Published: February 2024

AbstractIn a highly informative society, digital transformation has become a new driving force to enhance enterprise competitiveness and promote high‐quality economic development. This paper examines the impact of digital transformation on firms' resource allocation efficiency using a sample of Chinese A‐share listed companies in the manufacturing industry from 2007 to 2019. The research findings are as follows: The digital transformation has significantly improved enterprise resource allocation efficiency. Mechanisms suggest that digital transformation enhances resource allocation efficiency by attracting external financing, both equity and debt financing. Furthermore, the contribution of digital transformation to enterprise resource allocation efficiency is more pronounced in the eastern region, high‐tech industries, and non‐state‐owned enterprises. This paper provides new evidence for exploring effective paths to improve resource allocation efficiency from the digital transformation perspective. It is significant for accelerating enterprise digital transformation and achieving cost reduction and efficiency enhancement.
Digital economy policy and enterprise digital transformation: Evidence from innovation and structural effect
Authors: Xiao Deheng , Zhang Can , Huang Yongjian 
Published: February 2024

AbstractUsing a sample from 2010 to 2021 of listed companies in Shanghai and Shenzhen A‐shares, we explore the effect of digital economy policy (DEP) at city level on enterprise digital transformation (EDT). We find that DEP can promote EDT. And the outcome remains valid following a sequence of rigorous tests to assess its robustness and account for endogeneity. Further analysis indicates that DEP plays roles by internal innovation effect and external structural effect. Moreover, this positive effect will be stronger in enterprises in manufacturing and high‐tech industries and those with better staffing structure, higher enterprise productivity and value. Also, we find that the effect of DEP on enterprises varies in regions with different government subsidy, digital infrastructure level, and location.
Can the green experience of CEO improve ESG performance in heavy polluting companies? Evidence from China
Authors: Deng Mingjun , Tang Hao , Luo Wenbing 
Published: February 2024

AbstractThis paper focuses on Chief Executive Officers (CEOs) with green experience, using data from listed companies in China's heavy pollution industry spanning from 2011 to 2021, the study explores the impact of CEO green experience on the Environmental, Social, and Corporate Governance (ESG) performance of companies. The study reveals that CEO green experiences contribute to enhancing firms' ESG performance. The research further unveils that management's green perceptions and corporate green innovations play a partial mediating role in the relationship between CEO green experiences and corporate ESG performance. Heterogeneity analysis indicates that the influence of CEO green experiences on corporate ESG performance is more pronounced in samples characterized by high environmental regulation, capital‐intensive operations, and non‐state ownership. The findings of this study enrich the theory of corporate ESG performance power mechanisms and provide theoretical references for enhancing the ESG performance of heavily polluting firms.
Computed tomography‐based artificial intelligence in lung disease—Chronic obstructive pulmonary disease
Authors: Wang Fangfei , Li Sixiang , Gao Yuanxu , Li Shiyue 
Published: February 2024

AbstractChronic obstructive pulmonary disease (COPD) stands as a global health crisis, responsible for substantial morbidity and mortality on a worldwide scale. Its insidious nature underscores the importance of early detection and accurate diagnosis. While spirometry has been the cornerstone for COPD diagnosis, the role of computed tomography (CT) imaging has evolved, offering a valuable avenue for early detection and subtype classification. Recently, the advent of artificial intelligence (AI) has brought forth the potential to revolutionize the accuracy and efficiency of COPD diagnosis, with a specific focus on CT images. This intersection of healthcare and technology signifies a paradigm shift in the way we approach COPD management. The transformative capacity of AI positions it as a vital instrument for early detection and precise subtype classification of COPD. Moreover, the synergistic relationship between medical imaging and AI paves the way for more precise and efficient disease management. Therefore, in this perspective, we tend to offer a comprehensive exploration of the latest breakthroughs in the field of CT‐based AI in COPD diagnosis, aiming to demonstrate the promise and potential of AI in refining the accuracy of COPD classification and to illuminate the evolving landscape of AI's impact on COPD management.
Dynamics of eugenol included in β‐cyclodextrin by nuclear magnetic resonance and molecular simulations
Authors: Hernández‐Tanguma Alejandro , Ariza‐Castolo Armando 
Published: February 2024

AbstractEugenol–β‐cyclodextrin complex has been widely used because of the enhanced stability and conservation of the properties of eugenol. Applications in food and health sciences have been shown previously, which makes this complex an excellent model to understand and develop methodologies for the analysis and prediction of physical properties. In this work, the dynamics of eugenol incorporated into β‐cyclodextrin are presented, using NMR relaxation rates, and the predictive capabilities of molecular dynamics simulations are discussed. Results show a hindered rotation of eugenol around the principal inertial axes when located inside β‐cyclodextrin. Moreover, a translational movement of the whole complex is demonstrated.
A security‐enhanced equipment predictive maintenance solution for the ETO manufacturing
Authors: Cao Xiangyu , Jing Zhengjun , Zhao Xiaorong , Xu Xiaolong 
Published: February 2024

SummaryWith the rapid advancement of intelligent manufacturing, ensuring equipment safety has become a crucial prerequisite for enterprise production. In the engineer‐to‐order (ETO) production mode, characterized by diverse equipment types and frequent adjustments in production lines, equipment maintenance has become increasingly complex. Traditional maintenance plans are no longer adequate to meet the evolving demands of equipment maintenance. This paper proposes a security‐enhanced predictive maintenance scheme specifically designed for ETO‐type production equipment. The scheme utilizes industrial Internet of Things (IIoT) technology to monitor machines and equipment, constructs prediction models using machine learning methods, and reinforces the security of the prediction system through adoption of a decentralized architecture with blockchain distributed storage. In this experiment, six supervised learning models were compared, and it was found that the model based on the random forest algorithm achieved an outstanding accuracy rate of 98.88%. Furthermore, the average total response time for generating predictions within the system is 2.0 s, demonstrating a performance suitable for practical equipment maintenance applications.
Reduced dopamine transporter availability in drug‐naive adult attention‐deficit/hyperactivity disorder
Authors: Itagaki Shuntaro , Ohnishi Takashi , Toda Wataru , Sato Aya , Matsumoto Junya , Ito Hiroshi , Ishii Shiro , Yamakuni Ryo , Miura Itaru , Yabe Hirooki 
Published: February 2024

AbstractAimThis study aimed to clarify the abnormalities in dopamine transporter (DAT) availability in drug‐naive adult patients with attention‐deficit/hyperactivity disorder (ADHD) and the relationship between ADHD symptoms and abnormalities in DAT availability.MethodsSingle‐photon emission tomography (SPECT) was performed using iodine‐123‐β‐carbomethoxy‐3β‐(4‐iodophenyltropane) (I‐123 β CIT) as a tracer to measure in vivo DAT availability in 20 drug‐naive patients with ADHD [mean age ± standard deviation (SD)]: 25 ± 3.44 years; male:female = 11:9] and 20 age‐ and sex‐matched healthy controls (HCs) (mean age ± SD: 23.9 ± 2.27 years). Comparisons of DAT availability between HCs and adult patients with ADHD and the association between symptom severity and DAT availability within the ADHD group were analyzed using Statistical Parametric Mapping 12.ResultsDrug‐naive adults with ADHD showed significantly reduced DAT availability in the bilateral nucleus accumbens compared with HCs. Correlation analyses revealed a negative correlation between the severity of inattentive symptoms in adult patients with ADHD and DAT availability in the bilateral heads of the caudate nucleus, indicating the association between severe inattentive symptoms and lower DAT availability in the caudate nucleus.ConclusionIn drug‐naive adult patients with ADHD, DAT availability was reduced in the nucleus accumbens, an important part of the reward system. This finding indicates the importance of the DAT in the reward system in the pathogenesis of ADHD. Inattentiveness was associated with DAT availability in the caudate nucleus, suggesting involvement of the cortico‐striato‐thalamo‐cortical circuit.
Long‐term outdoor study of an organic photovoltaic module for building integration
Authors: Luo Wei , Khaing Aung Myint , Rodriguez‐Gallegos Carlos David , Leow Shin Woei , Reindl Thomas , Pravettoni Mauro 
Published: February 2024

AbstractOrganic photovoltaics (OPV) has attracted tremendous attention as a promising alternative to silicon wafer‐based technologies for building integration. While significant progress has been achieved on the power conversion efficiency of OPV technologies, their field stability is rarely studied. This work investigates the field performance and reliability of a large‐area OPV module designed for building integration in tropical Singapore for 4.5 years. The device suffered more than 14% degradation in power at the standard testing conditions from the initial performance, largely due to losses in fill factor (−12% relative). During the monitoring period, it exhibited comparable performance to more conventional silicon PV technologies, with an average specific energy yield of about 4 kWh/kWp/day and an average performance ratio of 0.96. Excellent performance at low light conditions was also observed. However, its field performance was heavily impacted by soiling, which typically led to a 5 to 10% loss in the current output after several months. Further, the device's outdoor performance also showed a three‐stage degradation process, including (1) an initial slow degradation in the first 2 years (about −1%/year), (2) a stable period with negligible performance loss from Years 2 to 3.5, and (3) a rapid degradation in the last year (about −5%/year).
microRNA‐encoded peptides inhibit seed germination of the root parasitic plant Orobanche cumana
Authors: Tourneur Sabine , Combier Jean‐Philippe , Plaza Serge , Muños Stéphane , Delavault Philippe 
Published: February 2024

Societal Impact StatementThe root parasitic plant Orobanche cumana (sunflower broomrape) is one of the major pests of sunflower crops. Despite intense efforts to develop effective agricultural practices and breeding programs, selective control of broomrapes is still rare and ineffective in terms of sustainability. It is thus essential to develop new specific control methods against those pests. miRNA‐encoded peptides (miPEPs) are a new class of peptides regulating the expression of miRNAs and their corresponding target genes. This study demonstrates that certain miPEPs strongly inhibit the germination of broomrape seeds by regulating their miR gene, making them good candidates for use as biocontrol agents against this pathogen.Summary

Root parasitic plants of the Orobanchaceae family are a constant and growing threat to agriculture worldwide. Among them, the parasitic weed Orobanche cumana, the sunflower broomrape, causes significant losses to sunflower production in European‐Asian and North African countries. Despite the use of several conventional control methods against this pathogen, none has proved effective or durable, underlining the need to develop innovative strategies. miRNA‐encoded peptides (miPEPs) are regulatory peptides stimulating the expression of their own primary transcript of miRNA, and plant watering with those molecules leads to down‐regulating specifically miRNA target genes and altering plant physiology.
Through seed germination assays and qRT‐PCR analysis, we investigated the impact of exogenous treatments of synthetic miPEPs on broomrape seed germination.
First, we report that the conserved miRNA repertoire of O. cumana consists of 39 members. Thirty‐nine miPEPs were designed, synthetized, and assayed, 11 of which strongly inhibited O. cumana seed germination. Interestingly, miPEP319a showed the strongest inhibiting effect while miPEP319b did not. Three out of the four corresponding miR319 target genes showed upregulation after treatment with a germination stimulant, which was impaired by treatment with miPEP319a. This downregulation of expression is associated with an increase in the expression of the corresponding pri‐miR319a.
We reveal thus that the use of miPEPs can increase our knowledge of key molecular mechanisms underlying a complex parasite interaction and should provide a new phytosanitary method to control broomrape parasitism with highly specific and biodegradable natural substances.
HSV‐1 ICP0 dimer domain adopts a novel β‐barrel fold
Authors: McCloskey Erick , Kashipathy Maithri , Cooper Anne , Gao Philip , Johnson David K. , Battaile Kevin P. , Lovell Scott , Davido David J. 
Published: February 2024

AbstractInfected cell protein 0 (ICP0) is an immediate‐early regulatory protein of herpes simplex virus 1 (HSV‐1) that possesses E3 ubiquitin ligase activity. ICP0 transactivates viral genes, in part, through its C‐terminal dimer domain (residues 555–767). Deletion of this dimer domain results in reduced viral gene expression, lytic infection, and reactivation from latency. Since ICP0's dimer domain is associated with its transactivation activity and efficient viral replication, we wanted to determine the structure of this specific domain. The C‐terminus of ICP0 was purified from bacteria and analyzed by X‐ray crystallography to solve its structure. Each subunit or monomer in the ICP0 dimer is composed of nine β‐strands and two α‐helices. Interestingly, two adjacent β‐strands from one monomer “reach” into the adjacent subunit during dimer formation, generating two β‐barrel‐like structures. Additionally, crystallographic analyses indicate a tetramer structure is formed from two β‐strands of each dimer, creating a “stacking” of the β‐barrels. The structural protein database searches indicate the fold or structure adopted by the ICP0 dimer is novel. The dimer is held together by an extensive network of hydrogen bonds. Computational analyses reveal that ICP0 can either form a dimer or bind to SUMO1 via its C‐terminal SUMO‐interacting motifs but not both. Understanding the structure of the dimer domain will provide insights into the activities of ICP0 and, ultimately, the HSV‐1 life cycle.
N‐Phenylnorbornenesuccinimide derivatives, agricultural defensive, and enzymatic target selection
Authors: Gomes Sabriny F , Alvarenga Elson S , Baia Vitor C , Oliveira Denilson F 
Published: February 2024

AbstractBACKGROUNDFaced with the need to develop new herbicides with modes of action different to those observed for existing agrochemicals, one of the most promising strategies employed by synthetic chemists involves the structural modification of molecules found in natural products. Molecules containing amides, imides, and epoxides as functional groups are prevalent in nature and find extensive application in synthesizing more intricate compounds due to their biological properties. In this context, this paper delineates the synthesis of N‐phenylnorbornenesuccinimide derivatives, conducts biological assays, and carries out in silico investigation of the protein target associated with the most potent compound in plant organisms. The phytotoxic effects of the synthesized compounds (2–29) were evaluated on Allium cepa, Bidens pilosa, Cucumis sativus, Sorghum bicolor, and Solanum lycopersicum.RESULTSReaction of endo‐bicyclo[2.2.1]hept‐5‐ene‐3a,7a‐dicarboxylic anhydride (1) with aromatic amines led to the N‐phenylnorbornenesuccinic acids (2–11) with yields ranging from 75% to 90%. Cyclization of compounds (2–11) in the presence of acetic anhydride and sodium acetate afforded N‐phenylnorbornenesuccinimides (12–20) with yields varying from 65% to 89%. Those imides were then subjected to epoxidation reaction to afford N‐phenylepoxynorbornanesuccimides (21–29) with yields from 60% to 90%. All compounds inhibited the growth of seedlings of the plants evaluated. Substance 23 was the most active against the plants tested, inhibiting 100% the growth of all species in all concentrations. Cyclophilin was found to be the enzymatic target of compound 23.CONCLUSIONThese findings suggest that derivatives of N‐phenylnorbornenesuccinimide are promising compounds in the quest for more selective and stable agrochemicals. This perspective reinforces the significance of these derivatives as potential innovative herbicides and emphasizes the importance of further exploring their biological activity on weeds. © 2024 Society of Chemical Industry.