bluuebunny/crossref_metadata_2025_split huggingface dataset:
Admissions to paediatric medical wards with a primary mental health diagnosis: a systematic review of the literature
Authors: Vázquez-Vázquez Adriana , Smith Abigail , Gibson Faith , Roberts Helen , Mathews Gabrielle , Ward Joseph Lloyd , Viner Russell M , Nicholls Dasha , Cornaglia Francesca , Roland Damian , Phillips Kirsty , Hudson Lee D 
Published: February 2024

ObjectiveTo systematically review the literature describing children and young people (CYP) admissions to paediatric general wards because of primary mental health (MH) reasons, particularly in MH crisis.DesignPubMed, Embase, PsycINFO, Web of Science and Google Scholar were searched, with no restriction on country or language. We addressed five search questions to inform: trends and/or the number of admissions, the risk factors for adverse care, the experiences of CYP, families/carers and healthcare professionals (HCPs) and the evidence of interventions aimed at improving the care during admissions.Two reviewers independently assessed the relevance of abstracts identified, extracted data and undertook quality assessment. This review was registered with PROSPERO (CRD42022350655).ResultsThirty-two studies met the inclusion criteria. Eighteen addressed trends and/or numbers/proportions of admissions, 12 provided data about the views/experiences of HCPs, two provided data about CYP’s experiences and four explored improving care. We were unable to identify studies examining risk factors for harm during admissions, but studies did report the length of stay in general paediatric/adult settings while waiting for specialised care, which could be considered a risk factor while caring for this group.ConclusionsMH admissions to children’s wards are a long-standing issue and are increasing. CYP will continue to need to be admitted in crisis, with paediatric wards a common location while waiting for assessment. For services to be delivered effectively and for CYP and their families/carers to feel supported and HCPs to feel confident, we need to facilitate more integrated physical and MH pathways of care.PROSPERO registration numberCRD42022350655.
Investigations and management of complex congenital talipes equinovarus
Authors: Novintan Shonnelly , Campioni-Norman Daniel , Hulme Alison 
Published: February 2024

Congenital talipes equinovarus (CTEV) is a congenital deformity affecting the feet, commonly idiopathic in nature. We present a previously unreported cause of a non-idiopathic clubfoot and highlight the importance of poor response to initial treatment.A poor response to Ponseti serial casting for CTEV should alert a clinician to the fact that the foot may not be in the ‘idiopathic’ group and be of a more complex nature. Idiopathic clubfoot should correct with a maximum of eight serial manipulations, cast applications and Achilles tendon tenotomy. If this is not the case, a repeat careful history, full examination, further investigations and review of the treatment method are required.
Respiratory symptoms, exacerbations and sleep disturbances are more common among participants with asthma and chronic airflow limitation: an epidemiological study in Estonia, Iceland and Sweden
Authors: Mindus Stephanie , Gislason Thorarinn , Benediktsdottir Bryndis , Jogi Rain , Moverare Robert , Malinovschi Andrei , Janson Christer 
Published: February 2024

BackgroundChronic airflow limitation (CAL) is a hallmark of chronic obstructive pulmonary disease but is also present in some patients with asthma. We investigated respiratory symptoms, sleep and health status of participants with and without CAL with particular emphasis on concurrent asthma using data from adult populations in Iceland, Estonia and Sweden investigated within the Burden of Obstructive Lung Disease study.MethodsAll participants underwent spirometry with measurements of forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) before and after bronchodilation. CAL was defined as postbronchodilator FEV1/FVC below the lower limit of normal. IgE-sensitisation and serum concentrations of eosinophil-derived neurotoxin (S-EDN) were assessed in a subsample. The participants were divided into four groups: no self-reported doctor’s diagnosed asthma or CAL, asthma without CAL, CAL without asthma and asthma and CAL: χ2test and analysis of variance were used in bivariable analyses and logistic and linear regression when analysing the independent association between respiratory symptoms, exacerbations, sleep-related symptoms and health status towards CAL, adjusting for centre, age, sex, body mass index, smoking history and educational level.ResultsAmong the 1918 participants, 190 (9.9%) had asthma without CAL, 127 (6.6%) had CAL without asthma and 50 (2.6%) had CAL with asthma. Having asthma with CAL was associated with symptoms such as wheeze (adjusted OR (aOR) 6.53 (95% CI 3.53 to 12.1), exacerbations (aOR 12.8 (95% CI 6.97 to 23.6), difficulties initiating sleep (aOR 2.82 (95% CI 1.45 to 5.48), nocturnal gastro-oesophageal reflux (aOR 3.98 (95% CI 1.79 to 8.82)) as well as lower physical health status. In these analyses, those with no asthma and no CAL were the reference group. The prevalence of IgE-sensitisation was highest in both asthma groups, which also had higher levels of S-EDN.ConclusionIndividuals with self-reported asthma with CAL suffer from a higher burden of respiratory and sleep-related symptoms, higher exacerbation rates and lower health status when compared with participants with asthma alone or CAL alone.
Continuous rise in oxygen consumption during prolonged military loaded march in the heat with and without fluid replacement: a pilot study
Authors: Wheelock Courtney E. , Stooks J , Schwob J , Hostler D 
Published: February 2024

IntroductionV̇O2drift, the rise in oxygen consumption during continuous exercise, has not been adequately reported during prolonged military marches. The purpose of this study was to analyse V̇O2and energy expenditure (EE) during a loaded march with and without rehydration efforts. Second, the study aimed to compare EE throughout the march with predicted values using a validated model.MethodsSeven healthy men (23±2 years; V̇O2max: 50.8±5.3 mL/kg/min) completed four 60 min loaded marches (20.4 kg at 50% V̇O2max) in a warm environment (30°C and 50% relative humidity). Three were preceded by hypohydration via a 4-hour cold water immersion (18°C). The control (CON) visit was a non-immersed euhydrated march. After water immersion, subjects were rehydrated with 0% (NO), 50% (HALF) or 100% (FULL) of total body mass lost. During exercise, V̇O2and EE were collected and core temperature change was calculated. To determine if EE could be accurately predicted, values were compared with a calculated estimate using the US Army Load Carry Decision Aid (LCDA).ResultsAt the start of exercise, there was no difference between conditions in V̇O2(ALL: 24.3±0.3 mL/kg/min; p=0.50) or EE (ALL: 8.6±1.0 W/kg; p=0.68). V̇O2(p=0.02) and EE (p<0.01) increased during exercise and were 12.3±10.0% and 12.8±9.5% greater, respectively, at 60 min across all trials and were not mitigated by rehydration amount. There was an effect of core temperature change on V̇O2for each condition (CON: r=0.62; NO: r=0.47; HALF: r=0.70; FULL: r=0.55). LCDA-predicted values were different from measured EE during exercise.ConclusionV̇O2drift occurred during loaded military marches and was associated with increases in EE and core temperature change. Pre-exercise hypohydration with water immersion followed by rehydration did not influence the degree of drift. LCDA prediction of EE may not agree with measured values during prolonged loaded marches where V̇O2drift occurs.
Plantar compartment block for hallux valgus surgery: a proof-of-concept anatomic and clinical study
Authors: Herteleer Matthias , Choquet Olivier , Swisser Fabien , Bernard Nathalie , Gasc Audrey , Canovas François , Dagneaux Louis , Bringuier Sophie , Capdevila Xavier 
Published: February 2024

BackgroundHallux valgus surgery is associated with moderate to severe postoperative pain. We hypothesized that a plantar compartment block may be a good technique for postoperative analgesia. We describe an anatomic approach to ultrasound-guided plantar compartment block and assess the clinical efficacy of the block for outpatient surgery.MethodsThe anatomic study was aimed to describe the plantar compartment, using both dissection methods and imaging, and to define a volume of local anesthetic. Patients scheduled for hallux valgus surgery with a popliteal sciatic nerve block, and combined plantar compartment and peroneal blocks were included in the clinical study. Data on attaining the criteria for rapid exit from the outpatient center, duration of sensory and analgesic block, visual analog scale (VAS) values for postoperative pain at rest and during movement, and the consumption of morphine as rescue analgesia were recorded.ResultsPlane-by-plane dissections and cross-sections were done in five cadaveric lower limbs. The medial calcaneal nerve divides into medial plantar and lateral plantar nerves in the upper part of the plantar compartment. These nerves were surrounded by 5 mL of colored gelatin, and 10 mL of injectates dye spread to the medial calcaneal branches. Thirty patients (26 women) were included in the clinical study. There were no failures of surgical block. Ninety per cent of patients successfully passed functional testing for ambulatory exit from the center within 5 hours (25th–75th centiles, 3.8–5.5 hours). The median duration of plantar compartment sensory block was 17.3 hours (10.5–21.5 hours), and the first request for rescue analgesic was 11.75 hours (10.5–23 hours) after surgery. The median VAS score for maximum pain reported within the 48-hour period was 2 (1–6). Twelve patients received 2.5 mg (0–5 mg) of morphine on day 1. Patients were highly satisfied and no adverse events were noted.ConclusionsThis anatomic description of the ultrasound-guided plantar compartment block reported the injection area to target the medial and lateral plantar nerves with 5 mL of local anesthetic. Normal walking without assistance is attained rapidly with this regional anesthesia technique, and the time to request postoperative analgesia after hallux valgus surgery is long.Trial registration numberNCT03815422.
Airway epithelial cell response to RSV is mostly impaired in goblet and multiciliated cells in asthma
Authors: Gay Aurore C A , Banchero Martin , Carpaij Orestes , Kole Tessa M , Apperloo Leonie , van Gosliga Djoke , Fajar Putri Ayu , Koppelman Gerard H , Bont Louis , Hendriks Rudi W , van den Berge Maarten , Nawijn Martijn C 
Published: February 2024

BackgroundIn patients with asthma, respiratory syncytial virus (RSV) infections can cause disease exacerbation by infecting the epithelial layer of the airways, inducing subsequent immune response. The type I interferon antiviral response of epithelial cells upon RSV infection is found to be reduced in asthma in most—but not all—studies. Moreover, the molecular mechanisms causing the differences in the asthmatic bronchial epithelium in response to viral infection are poorly understood.MethodsHere, we investigated the transcriptional response to RSV infection of primary bronchial epithelial cells (pBECs) from patients with asthma (n=8) and healthy donors (n=8). The pBECs obtained from bronchial brushes were differentiated in air-liquid interface conditions and infected with RSV. After 3 days, cells were processed for single-cell RNA sequencing.ResultsA strong antiviral response to RSV was observed for all cell types, for all samples (p<1e-48). Most (1045) differentially regulated genes following RSV infection were found in cells transitioning to secretory cells. Goblet cells from patients with asthma showed lower expression of genes involved in the interferon response (false discovery rate <0.05), includingOASL,ICAM1andTNFAIP3. In multiciliated cells, an impairment of the signalling pathways involved in the response to RSV in asthma was observed.ConclusionOur results highlight that the response to RSV infection of the bronchial epithelium in asthma and healthy airways was largely similar. However, in asthma, the response of goblet and multiciliated cells is impaired, highlighting the need for studying airway epithelial cells at high resolution in the context of asthma exacerbation.
Positron scattering from neon and argon
Authors: Machacek J. R. , McEachran R. P. 
Published: February 2024

Abstract
The relativistic optical potential method has been used to calculate a complete set of cross sections for positron scattering from neon and argon in the energy range from zero to 1 keV. Elastic, positronium formation, excitation, direct ionization, and grand total cross sections are presented and compared to the recommended results of Ratnavelu et al. (J Phys Chem Ref Data 48:023102, 2019). Here, positronium formation is modeled as an ionization process by successively lowering the ionization thresholds. This process was carried out for the four lowest energy levels of positronium.

Graphical abstract
Electroformation of giant unilamellar vesicles from large liposomes
Authors: Uzun Huriye D. , Tiris Zeynep , Czarnetzki Maiko , López-Marqués Rosa L. , Günther Pomorski Thomas 
Published: February 2024

AbstractGiant unilamellar vesicles (GUVs) are widely used as model systems for biological membranes to study membrane-related processes in a precisely controlled in vitro environment, owing to their biophysical properties. The classical technique for the formation of giant liposomes starts with the dissolution of lipids or lipid mixtures in an organic solvent, which is then deposited as a thin lipid solution film on a support substrate. In this study, we present a comprehensive analysis investigating the effect of different lipid compositions on the generation of GUVs from preformed liposomes under non-ionic and ionic conditions. For all liposome types tested, the electroformation process, whether performed on indium tin oxide-coated glass slides or platinum wires, consistently produced GUVs that typically ranged in size from 5 to 20 μm. However, the yield of GUVs varied depending on the specific non-ionic or ionic conditions and the lipid composition of the preformed liposomes used. In general, the resulting population of giant vesicles was predominantly characterised by the presence of unilamellar and multivesicular vesicles. These findings have the potential to improve the refinement of protocol parameters for the formation of GUVs containing membrane proteins and for the study of the effects of lipid composition on membrane protein activity.
Targeting Dendritic Cell Dysfunction to Circumvent Anti-PD1 Resistance in Head and Neck Cancer
Authors: Saito Shin , Kono Michihisa , Nguyen Hoang C.B. , Egloff Ann Marie , Messier Cameron , Lizotte Patrick , Paweletz Cloud , Adkins Douglas , Uppaluri Ravindra 
Published: February 2024

Abstract

Purpose:
Neoadjuvant anti-PD1 (aPD1) therapies are being explored in surgically resectable head and neck squamous cell carcinoma (HNSCC). Encouraging responses have been observed, but further insights into the mechanisms underlying resistance and approaches to improve responses are needed.

Experimental Design:
We integrated data from syngeneic mouse oral carcinoma (MOC) models and neoadjuvant pembrolizumab HNSCC patient tumor RNA-sequencing data to explore the mechanism of aPD1 resistance. Tumors and tumor-draining lymph nodes (DLN) from MOC models were analyzed for antigen-specific priming. CCL5 expression was enforced in an aPD1-resistant model.

Results:
An aPD1-resistant mouse model showed poor priming in the tumor DLN due to type 1 conventional dendritic cell (cDC1) dysfunction, which correlated with exhausted and poorly responsive antigen-specific T cells. Tumor microenvironment analysis also showed decreased cDC1 in aPD1-resistant tumors compared with sensitive tumors. Following neoadjuvant aPD1 therapy, pathologic responses in patients also positively correlated with baseline transcriptomic cDC1 signatures. In an aPD1-resistant model, intratumoral cDC1 vaccine was sufficient to restore aPD1 response by enhancing T-cell infiltration and increasing antigen-specific responses with improved tumor control. Mechanistically, CCL5 expression significantly correlated with neoadjuvant aPD1 response and enforced expression of CCL5 in an aPD1-resistant model, enhanced cDC1 tumor infiltration, restored antigen-specific responses, and recovered sensitivity to aPD1 treatment.

Conclusions:
These data highlight the contribution of tumor-infiltrating cDC1 in HNSCC aPD1 response and approaches to enhance cDC1 infiltration and function that may circumvent aPD1 resistance in patients with HNSCC.
Effects of lower limb muscle strengthening on interface pressure in older adults undergoing inelastic compression: Randomized controlled clinical trial
Authors: Da Matta Eduardo Simões , Mosti Giovanni , Corralo Vanessa da Silva , Moura Glauciano Policeno de , Quadros Luciano Branco de , De Sá Clodoaldo Antônio 
Published: February 2024

Objective To analyze the effects of lower limb muscle strengthening on interface pressure (IP), static stiffness index (SSI), dynamic stiffness index (DSI), and walking pressure amplitude (WPA) in older adults undergoing inelastic compression therapy. Method Forty-three healthy older adults of both sexes (Age: 66.2 ± 4.4 years) met the eligibility criteria and completed all stages of the study (Resistance Training – RT: N = 20; Control – CONT: N = 23). Body composition, strength and functional mobility of lower limbs, IP, SSI, DSI, and WPA were evaluated before and after intervention. Results The two-way ANOVA with repeated measures demonstrated a significant time-group interaction effect on muscular strength for 1-RM [F (1, 41) = 21.091; p ≤ 0.001], IP in the orthostatic position [F (1, 41) = 5.124; p ≤ 0.05], minimum WPA [F (1, 41) = 10.999; p ≤ 0.05], maximum WPA [F (1, 41) = 8.315; p ≤ 0.05], DSI minimum (F (1, 41) = 4.608; p ≤ 0.05), DSI maximum (F (1, 41) = 8.926; p ≤ 0.05), and on the delta DSI (F (1, 41) = 7.891; p ≤ 0.05). Conclusions In RT group, the increase in lower limb muscle strength was accompanied by an increase in IP in foot dorsiflexion and plantar flexion maneuvers, both in the standing position (DSI) and during gait (WPA).
‘So being here is. . . I feel like I’m being a social worker again, at the hospice’: Using interpretative phenomenological analysis to explore social workers’ experiences of hospice work
Authors: Scanlon Hayley , Latchford Gary , Allsop Matthew 
Published: February 2024

Background: Social workers have a significant role in hospices working with clients who are facing death but there is limited detailed understanding of the emotional impact of this work on social workers. Research has highlighted that those involved in hospice work find the work both a struggle (e.g. because of heightened emotions) and rewarding (noting that end-of-life care can feel like a privilege). Aim: To explore UK hospice social workers’ emotional experiences of work and how this influences their practice. Design: Semi-structured interviews were conducted with hospice social workers. Interviews were transcribed and transcripts were analysed using Interpretative Phenomenological Analysis. Setting/participants: Eight social workers from different hospices in the UK. Results: Five overlapping superordinate themes emerged: making a difference to clients and families (‘the difference made’), the emotional impact of working in hospices (‘dealing with people’s emotions, and death, and dying, it’s serious stuff’), the relational context of this type of work (‘awareness of affinity to connect’), the ways in which coping is facilitated in hospices (‘seen it coming’) and a foundation theme, connection and disconnection to values (‘(dis)connection to values’). Conclusions: The results offer an exploration of social workers’ experiences of their work in hospices; how adept they were at coping and how they prepared for and made sense of the often emotionally-laden experiences encountered. Their experience of the rewards and meaning derived from their work offers important findings for clinical practice. Further research is suggested to explore a multitude of healthcare professionals’ perspectives across country settings using Interpretative Phenomenological Analysis.
Melanoma and sex hormones: Pathogenesis, progressive disease and response to treatments
Authors: Taglialatela Ida , Indini Alice , Santanelli Giulia , Di Liberti Giorgia , Di Guardo Lorenza , De Braud Filippo , Del Vecchio Michele 
Published: February 2024

Cutaneous melanoma represents the fifth tumor in terms of incidence in young adults, with a major involvement of males than females. Despite the significant changes in available effective treatments for cutaneous melanoma, there is still a proportion of patients that do not benefit long-term disease control with immune checkpoint inhibitors and/or BRAF/MEK inhibitors, and eventually develop progressive disease. In addition to the emerging biomarkers under investigation to understand resistance to treatments, recent studies resumed the role of sex hormones (estrogens, progesterone and androgens) in melanoma patients. In the last decades, the impact of sex hormones has been considered controversial in melanoma patients, but actual growing preclinical and clinical evidence underline the potential influence on melanoma cells’ growth, tumor microenvironment, the immune system and consequently on the course of disease. This review will provide available insights on the role of sex hormones in melanoma pathogenesis, disease progression and response/resistance to systemic treatments. We will also offer an overview on the recent studies on the theme, describing the hormonal contribution to disease response and the interaction with targeted therapies and immune-checkpoint inhibitors in cutaneous melanoma patients, illustrating an insight into future research in this field.