bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morpheus – An innovative approach to remote data access
Authors: Höninger Julia 
Published: January 2025

Morpheus is a novel approach in providing remote access to micro data of official statistics. Researchers work on anonymous micro data files with common statistical software packages and get their results back in real time. Additionally, a measure of goodness of fit will be provided for every single result. Therefore researchers can work with the anonymous results as they can have confidence that they would have obtained the same or very similar results with the original data. All statistical analyses and all commands are allowed. Furthermore, users can browse through the anonymous data which is very helpful when developing program syntax and not possible in most other systems of remote access. Research data centres would greatly benefit from such a system as well, as the cumbersome manual disclosure control would be eliminated. All results would be safe and automatically returned to the researcher. This system would respect the special requirements to micro data access even in Germany where laws are especially strict.
PARTAI ISLAM DAN PEMILU 2024
Authors: Danis Tri Saputra  , M.Prakoso Aji  , Ilmar Anwar , Juned Mansur 
Published: January 2025

Perkembangan partai politik Islam di Indonesia bergerak secara fluktuatif, namun kemampuan elektabilitasnya selalu berada jauh dari partai nasionalis, persentase suaranya cenderung stagnan dan tetap sulit bersaing dengan partai-partai nasionalis. Kondisi ini didorong oleh berbagai faktor yaitu konflik internal, kasus korupsi, isu radikalisme, lemahnya institusionalisasi partai, kaderisasi yang tidak berkelanjutan, program politik kerakyatan yang bias, peluasan paham Islam moderat, penguatan ormas Islam dan ekspansi elektoral partai-partai nasionalis. Penelitian ini dilakukan dengan menggunakan pendekatan mix methode dengan metode sequential exploratory yang memadukan analisis kualitatif dengan analisis kuantitatif. Penelitian ini menemukan bahwa hampir semua partai politik Islam di Indonesia mampu membaca berbagai peluang, ancaman, kekurangan dan kelebihan dari partai Islam di Indonesia pada pemilu 2024, sehingga sebagian besar strateginya lebih banyak didominasi oleh strategi ofensif, defensif, campuran serta kampanye politik secara bersamaan melalui strategi kampanye sebagai partai politik terbuka, strategi kampanye politik programatik, strategi politik oposisi dan koalisi, perluasan segmentasi pemilih muda dan penguatan kaderisasi internal serta upaya-upaya konstruktif dalam menghadapi politik identitas yang berbasis SARA
Citronellol-functionalized natural silica: a biogenic approach for antifungal and antibacterial material applications
Authors: Lopez Guillermo P. , Roque Leyanet Barberia , Igal Katerine , Espinosa Erasmo Gámez , Bellotti Natalia 
Published: January 2025

IntroductionNew bioactive hybrid materials to prevent biofilm-induced biodeterioration are a significant challenge in indoor environments, where contaminants from microbial films compromise structural integrity and contribute to air pollution, posing health risks from prolonged exposure to biological agents.MethodsFor the first time, diatomaceous earth or diatomite (Dt) was functionalized with quaternary ammonium salt (QAS) and a biogenic compound, citronellol, to develop a bioactive hybrid material (Dt*QC). The hybrids obtained were characterized by thermogravimetric analysis (TGA), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and Fourier-transform infrared spectroscopy (FTIR). The antifungal and antibacterial activity were assessed by agar diffusion assay, and micro/macro-dilution tests.Results and DiscussionCharacterization confirmed successful functionalization. TGA revealed organic contents of 50.9% with citronellol incorporation reaching 48.1%. SEM-EDS corroborated the incorporation of organic components. FTIR further verified the integration of functional groups while preserving the structural stability of the siliceous framework. Antimicrobial assays revealed a broader range of activity for Dt*QC. For bacterial strains, Dt*QC achieved a minimum inhibitory concentration (MIC) of 0.15 mg/mL against Staphylococcus aureus and demonstrated over 99.9% bacterial reduction, even at lower concentrations. This study highlights a novel approach to developing antimicrobial materials by functionalizing Dt with QAS and citronellol. Overall, these findings underscore the potential of Dt*QC as an advanced antimicrobial material for applications in coatings and preservation systems, offering a sustainable solution to prevent biodeterioration and microbial contamination.
Retrospective analysis of 300 microbial cell-free DNA sequencing results in routine blood stream infection diagnostics
Authors: Neidhöfer Claudio , Klein Niklas , Yürüktümen Aylin , Hattenhauer Tessa , Mispelbaum Rebekka , Bode Christian , Holderried Tobias A. W. , Hoerauf Achim , Parčina Marijo 
Published: January 2025

IntroductionBloodstream infections are a critical challenge worldwide due to the slow turnaround time of conventional microbiological tests for detecting bacteremia in septic patients. Noscendo GmbH (Duisburg, Germany) has developed the CE/IVD pipeline DISQVER for clinical metagenomics testing based on cell-free DNA (cfDNA) from blood samples to address this issue.MethodsWe conducted a retrospective study to evaluate the diagnostic utility of this methodological setup in improving treatment decisions since it was introduced into our clinical setting. Between January 2021 and June 2022, the first 300 cases in which DISQVER was applied at our university hospital were collected and analyzed. The results were compared with routine microbiology test results, clinical picture, associated treatment decisions, and clinical course.ResultsOur findings demonstrate that DISQVER results where no pathogen was reported effectively ruled out bacterial bloodstream infections, whereas positive results varied in their usefulness. While the metagenomic approach proved highly valuable for detecting non-culturable and rare pathogens, its utility was limited in cases where detected microorganisms were commonly associated with the microbiota.DiscussionPerforming on-site analysis might mitigate delays resulting from logistical challenges and might help optimizing antibiotic stewardship. Once prompt results can be obtained, the relevance of incorporating molecular resistograms will become more pronounced. Further, the specific patient subgroups that most benefit from this analysis must be worked out. Guiding clinicians in identifying the infection focus based on the detected bacteria would significantly improve patient care. Lastly, evidence of filamentous fungi must be diligently followed up.
Analysis of regional differences, spatial-temporal characteristics, and influencing factors of the coupling and coordination development of science and technology innovation between universities and enterprises in China
Authors: Liu Xiao , Wang Qingjin , Gao Hao , Cui Qian 
Published: January 2025

IntroductionThe coupling and coordination development level (CCDL) of science and technology innovation (STI) between universities and enterprises is a key determinant of the effectiveness of the national STI system.MethodsThis study utilized data from universities and industrial enterprises spanning from 2012 to 2021 to establish an indicator system for measuring university STI ability and enterprise STI ability. The study then applied the entropy TOPSIS method to calculate the STI scores and further analyzed them using a coupling coordination model. Various research methods were employed to quantify the spatiotemporal characteristics and elucidate the patterns of evolution, with the grey relational analysis model being utilized to examine the mutual influence at the micro-index level.Results and discussionThe study found a significant transformation in the collaboration between universities and enterprises in China, shifting from extreme incoherence to highly coordinated cooperation. Secondly, the reason for the differences is the uneven development in the four regions. Thirdly, the CCDL of STI between universities and enterprises in each region is notably different, but all show σ convergence trend. Finally, the university STI ability and enterprise STI ability are identified as key factors in the CCDL of STI between universities and enterprises.
Systematic review of innate immune responses against Mycobacterium tuberculosis complex infection in animal models
Authors: Nieto Ramirez Luisa Maria , Mehaffy Carolina , Dobos Karen Marie 
Published: January 2025

BackgroundMycobacterium tuberculosis (Mtb) complex (MTBC) includes ten species that affect mammals and pose a significant global health concern. Upon infection, Mtb induces various stages in the host, including early bacterial elimination, which may or may not involve memory responses. Deciphering the role of innate immune responses during MTBC infection is crucial for understanding disease progression or protection. Over the past decade, there has been growing interest in the innate immune response to Mtb, with new preclinical models emerging.MethodsWe conducted a systematic review following PRISMA guidelines, focused on innate immune mediators linked to protection or disease progression in animal models of MTBC infection. We searched two databases: National Library of Medicine and Web of Science. Two researchers independently extracted data based on specific inclusion and exclusion criteria.ResultsEighty-three articles were reviewed. Results were categorized in four groups: MTBC species, animal models, soluble factors and innate pathways, and other molecules (metabolites and drugs). Mtb and M. bovis were the only species studied. P2X7R receptor's role in disease progression and higher macrophage recruitment were observed differentially after infection with hypervirulent Mtb strains. Mice and non-human primates (NHPs) were the most used mammals, with emerging models like Galleria mellonella and planarians also studied. NHPs provided insights into age-dependent immunity and markers for active tuberculosis (ATB). Key innate immune factors/pathways identified included TNF-α, neutrophil recruitment, ROS/RNS responses, autophagy, inflammasomes, and antimicrobial peptides, with homologous proteins identified in insects. Metabolites like vitamin B5 and prostaglandin E2 were associated with protection. Immunomodulatory drugs targeting autophagy and other mechanisms were studied, exhibiting their potential as therapeutic alternatives.ConclusionSimpler, physiologically relevant, and ethically sound models, such as G. mellonella, are needed for studying innate responses in MTBC infection. While insects lack adaptive immunity, they could provide insights into “pure” innate immune responses. The dissection of “pure,” “sustained” (later than 7 days post-infection), and trained innate immunity presents additional challenges that require high-resolution temporospatial analytical methods. Identifying early innate immune mediators and targetable pathways in the blood and affected tissues could identify biomarkers for immunization efficiency, disease progression, and potential synergistic therapies for ATB.
Deregulated methylation and expression of PCDHGB7 in patients with non-small cell lung cancer: a novel prognostic and immunological biomarker
Authors: Yuan Yue , Nie Xin , Gao Jiayi , Tian Yumeng , He Liuer , Wang Xue , Zhang Ping , Ma Junling , Li Lin 
Published: January 2025

BackgroundsProtocadherin gamma subfamily B, 7 (PCDHGB7), a member of the protocadherin family, plays critical roles in neuronal connections and has been implicated in female reproductive system cancers. Its function in lung cancer has not been elucidated.MethodsWe comprehensively investigated PCDHGB7 expression, prognosis, biological function, methylation patterns, and it’s relationship with immune infiltration and immunotherapy response through public datasets (HPA, TCGA, GEO, OncoDB and MEXPRESS). Two lung cancer immunotherapy cohorts from our clinical center were enrolled to detect the relationship between methylation and protein levels of PCDHGB7 in plasma and immunotherapy outcomes.ResultsPCDHGB7 expression was downregulated in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) and associated with tumor prognosis. PCDHGB7 demonstrated a positive correlation with inhibitory immune cells and a negative correlation with tumor mutational burden (TMB) and homologous recombination deficiency (HRD). The methylation level of PCDHGB7 was upregulated in tumor tissue and negatively correlated with PCDHGB7 mRNA level. In immunotherapy cohort studies, patients with higher PCDHGB7 tissue expression showed worse prognosis. Patients with PCDHGB7 hypermethylation in baseline plasma had shorter progression-free survival (PFS) and overall survival (OS), while those with early reduction of PCDHGB7 methylation had the best prognosis. Plasma PCDHGB7 protein levels could predict responses to immune checkpoint inhibitors and function as a prognostic marker for PFS.ConclusionPCDHGB7 expression and methylation are prognostic and immunological biomarkers in non-small cell lung cancer. Plasma PCDHGB7 methylation and protein levels can be used as novel biomarkers for predicting the efficacy of immunotherapy in lung cancer.
Deciphering the functional roles of PE18 and PPE26 proteins in modulating Mycobacterium tuberculosis pathogenesis and immune response
Authors: Ehtram Aquib , Shariq Mohd , Quadir Neha , Jamal Salma , Pichipalli Manjunath , Zarin Sheeba , Sheikh Javaid Ahmad , Ehtesham Nasreen Z. , Hasnain Seyed E. 
Published: January 2025

IntroductionTuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a leading cause of mortality worldwide. A crucial factor in Mtb's virulence is the ESX-5 secretion system, which transports PE/PPE proteins such as PE18 and PPE26. These proteins modulate host-pathogen interactions, immune responses, and intracellular survival mechanisms. Despite their importance, the roles and molecular interactions of PE18 and PPE26 in Mtb pathogenesis require further investigation.MethodsWe explored the roles of PE18 and PPE26 using recombinant Mycobacterium smegmatis (Msmeg) as a model organism. Protein-protein interactions were analyzed biochemically to identify partners within the ESX-5 secretion system, including EspG5 and other PE/PPE proteins. Subcellular localization of these proteins was assessed via cell fractionation studies. Functional assays, including in vitro cytokine production and antigen presentation studies, were performed using TLR2/Myd88 knockout and wild-type macrophages. In vivo experiments were conducted to assess effector T-cell activation and intracellular survival. Mechanistic insights into endosome-phagosome maturation and actin cytoskeleton dynamics were obtained through fluorescence microscopy.ResultsOur biochemical analyses confirmed interactions between PE18/PPE26, PE18/PPE27, PE19/PPE25, and EspG5/PPE, highlighting their involvement in ESX-5-mediated secretion. Cell fractionation studies revealed that PE/PPE proteins predominantly localize to the cell wall, with PE18 also secreted extracellularly. In vitro and in vivo experiments demonstrated that PE18 and PPE26 activate cytokine production and antigen presentation via TLR2/Myd88-dependent signaling pathways, inducing robust effector memory T-cell responses. Recombinant Msmeg expressing PE18, PPE26, or their combination exhibited enhanced intracellular survival by disrupting endosome-phagosome maturation, likely through interference with actin cytoskeletal organization.DiscussionOur findings elucidate the pivotal roles of PE18 and PPE26 in Mtb pathogenesis, emphasizing their contributions to immune modulation and intracellular persistence. The observed disruption of actin dynamics and endosome-phagosome maturation underscores a novel mechanism by which Mtb evades host defenses. The ability of PE18 and PPE26 to induce effector T-cell responses highlights their potential as targets for host-directed therapies or vaccine development against TB. Further studies focusing on their structure-function relationships and interactions with host proteins could accelerate the development of innovative therapeutic strategies.
Case report: Pseudoprogression mimicking neoplastic recurrence three months after completion of proton beam therapy for an IDH-mutant astrocytoma CNS WHO grade 3
Authors: Heggebø Liv Cathrine , Borgen Ida Maria Henriksen , Blakstad Hanne , Saxhaug Cathrine , Rønning Pål André , Niehusmann Pitt Frederik , Werlenius Katja , Blomstrand Malin , Brandal Petter 
Published: January 2025

BackgroundRadiation-induced changes following proton beam therapy in isocitrate dehydrogenase (IDH)-mutated diffuse central nervous system (CNS) World Health Organization (WHO) grade 2 and 3 gliomas are not well characterized. We present a patient with an IDH-mutant astrocytoma CNS WHO grade 3 treated with proton beam therapy and with postradiation MRI changes suggestive of neoplastic progression that surprisingly turned out to be reactive.Case presentationA man in his twenties underwent surgery with a near gross total resection for what turned out to be an IDH-mutant astrocytoma CNS WHO grade 3. He was included in the PRO-GLIO trial and randomized to receive proton beam therapy to a total dose of 59.4 Gray (Gy) relative biological effectiveness (RBE). Four weeks after completion of radiotherapy, adjuvant temozolomide was commenced. All treatment was well tolerated, and the patient was in excellent general condition. Surprisingly, magnetic resonance imaging (MRI) examination three months after completion of radiotherapy showed what was highly suggestive of a distant recurrence. The patient underwent resective surgery about seven months after his first surgery. Histological examination showed inflammatory changes without neoplastic tissue, albeit not very typical for postradiation changes. Adjuvant chemotherapy with temozolomide was continued.ConclusionThe presented case clearly shows that caution must be taken when interpreting cerebral MRI changes postradiation, and in particular after proton therapy. Further understanding of this subject is crucial to distinguish between patients requiring intensified antineoplastic treatment and those for whom maintaining current therapy or ongoing watchful waiting is advisable.
Osteoradionecrosis in osseous free flaps after maxillofacial reconstruction: a single-center experience
Authors: Fenske Jakob , Lampert Philipp , Nikolaidou Eirini , Steffen Claudius , Beck Marcus , Neckel Norbert , Nahles Susanne , Heiland Max , Mrosk Friedrich , Koerdt Steffen , Rendenbach Carsten 
Published: January 2025

ObjectiveIn the multimodal treatment of advanced head and neck malignancies, primary free flap reconstruction in a one stage procedure with tumor resection is frequently combined with adjuvant radiotherapy. Radiotherapy is known to exhibit side effects on transplanted free flaps, including osteoradionecrosis (ORN) of native and transplanted bone. This study aims to evaluate the therapeutic outcomes and potential predictors of free flap ORN within osseous free flaps based on a large-scale, single-center database.MethodsA retrospective analysis was conducted on patients who underwent osseous free flap reconstruction of maxilla or mandible in a one stage procedure followed by adjuvant radiotherapy due to an advanced head and neck malignancy between April 2017 and July 2023. After case matching, patients with and without free flap ORN were assessed for potential predictors using univariate and multivariate analysis.Results112 patients met the inclusion criteria. 21 patients (19%) developed ORN within the free flap. Following case matching, 42 patients (10 females, mean age 61.5 ± 9.1 years) were included in the final analysis. The mean time to ORN diagnosis was 19 (7–56) months after surgery. Total flap loss occurred in 7 patients (33%) following flap ORN. Smoking (76% vs. 38%; OR 5.78; p=0.03) and prior plate exposure (67% vs. 24%; OR 5.61; p=0.03) emerged as robust predictors of flap ORN in uni- and multivariate analysis.ConclusionOsseous free flap ORN is a severe radiooncologic complication, often resulting in total flap loss and subsequently increased morbidity. Smoking and prior plate exposure were identified as key predictors of flap ORN development. Individual risk assessment and careful evaluation of osseous free flap irradiation must be evaluated in future radiooncological concepts.
The consistency and efficacy of optical coherence tomography for the evaluation of ocular torsion angle in children
Authors: Liu Rongjun , Zhao Jingjing , Yu Kaili , Zhu Dehai 
Published: January 2025

PurposeTo explore the consistency and efficacy of optical coherence tomography (OCT) for the evaluation of ocular torsion angle compared to fundus photography in children.MethodPatients who had undergone fundus photography and OCT within 1 day were included in this study. The fundus photographs were taken using a fundus camera and then imported into the Adobe Photoshop 2021 software. The fovea-disc angle (FDA) was calculated manually. OCT was used to automatically detect the centers of the optic disc and fovea, and the software then calculated the FDA on the same device. The means and ranges of variables were calculated in this study. Group differences were assessed using the paired t-test. Statistical significance was determined when P-values were &lt;0.05. Bland–Altman plots were constructed to verify the agreement of the FDAs measured by fundus photography and OCT respectively.ResultsA total of 100 patients were included and the objective measurements of the ocular torsion angles via OCT and fundus photography were similar. The mean FDA of 32 patients aged 2–6 years old was −7.84 ± 4.67° via fundus photography and −6.71 ± 6.19° via OCT. The mean FDA of 68 patients aged 6–18 years old was −8.47 ± 5.22° via fundus photography and −8.97 ± 5.41° via OCT. According to the receiver operational characteristic (ROC) curves of the FDA for diagnosing ocular extorsion, the area under the ROC curve was greatest for OCT (0.943, 95% CI: 0.902–0.984), followed by fundus photography (0.92, 95% CI: 0.86–0.979). With the Youden method, the optimal cut-off point for diagnosing ocular extorsion with OCT was −6.35°. OCT demonstrated a sensitivity of 100% and a specificity of 49.2%. Furthermore, the optimal cut-off point for diagnosing ocular extorsion with fundus photography was −6.5°.ConclusionThe comparison of FDAs showed good agreement between fundus photography and OCT. Thus, OCT can evaluate ocular torsion angle effectively in children.