bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cryo-EM structure of the NDH–PSI–LHCI supercomplex from Spinacia oleracea
Authors: Introini Bianca , Hahn Alexander , Kühlbrandt Werner 
Published: January 2025

Abstract
The nicotinamide adenine dinucleotide phosphate (NADPH) dehydrogenase (NDH) complex is crucial for photosynthetic cyclic electron flow and respiration, transferring electrons from ferredoxin to plastoquinone while transporting H+ across the chloroplast membrane. This process boosts adenosine triphosphate production, regardless of NADPH levels. In flowering plants, NDH forms a supercomplex with photosystem I, enhancing its stability under high light. We report the cryo-electron microscopy structure of the NDH supercomplex in Spinacia
oleracea at a resolution of 3.0–3.3 Å. The supercomplex consists of 41 protein subunits, 154 chlorophylls and 38 carotenoids. Subunit interactions are reinforced by 46 distinct lipids. The structure of NDH resembles that of mitochondrial complex I closely, including the quinol-binding site and an extensive internal aqueous passage for proton translocation. A well-resolved catalytic plastoquinone (PQ) occupies the PQ channel. The pronounced structural similarity to complex I sheds light on electron transfer and proton translocation within the NDH supercomplex.
A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth
Authors: Saunders Harriet A. J. , van den Berg Cyntha M. , Hoogebeen Robin A. , Schweizer Donna , Stecker Kelly E. , Roepman Ronald , Howes Stuart C. , Akhmanova Anna 
Published: January 2025

Abstract
Cilia are motile or sensory organelles present on many eukaryotic cells. Their formation and function rely on axonemal microtubules, which exhibit very slow dynamics, but the underlying mechanisms are largely unexplored. Here we reconstituted in vitro the individual and collective activities of the ciliary tip module proteins CEP104, CSPP1, TOGARAM1, ARMC9 and CCDC66, which interact with each other and with microtubules and, when mutated in humans, cause ciliopathies such as Joubert syndrome. We show that CEP104, a protein with a tubulin-binding TOG domain, and its luminal partner CSPP1 inhibit microtubule growth and shortening. Another TOG-domain protein, TOGARAM1, overcomes growth inhibition imposed by CEP104 and CSPP1. CCDC66 and ARMC9 do not affect microtubule dynamics but act as scaffolds for their partners. Cryo-electron tomography demonstrated that, together, ciliary tip module members form plus-end-specific cork-like structures that reduce protofilament flaring. The combined effect of these proteins is very slow processive microtubule elongation, which recapitulates axonemal dynamics in cells.
SOFCs integrated with SMES under dynamic power control using Chernobyl disaster optimizer
Authors: Selem Sameh I. , El-Fergany Attia A. , Gouda Eid A. , Kotb Mohamed F. , Ismael Islam 
Published: January 2025

Abstract
The current study uses the Chernobyl disaster optimizer (CDO), a new metaheuristic optimizer, to identify the seven unknown parameters of solid oxide fuel cells (SOFCs). The procedures of the CDO is based on physical behavior of the elaborated radiations from the well-known Chernobyl disaster according to their mass, speed, frequency, and degree of ionization. The sum of square errors (SMSE) among the estimated and the real measured output voltage datasets of SOFCs is minimized employing the CDO. Set of boundaries of the SOFC’s process is taken into consideration with the problem formulation. SOFCs stack’s model is examined at 800οC and 900οC and its performance is confirmed. The CDO extracts more precise SOFCs’ parameters compared to other competitors. The CDO’s convergence patterns and the SOFCs unit’s performance are studied and proved at steady-state by comparing its results to a number of recognized algorithms under varied operating scenarios. A significant SMSE’s values of 3.46 µV2 and 7.38 µV2 are attained at 800οC and 900οC, respectively by the CDO. As a result, the polarization principal curves of the measured and estimated voltage datasets are checked and verified with very close matching. The dynamic behavior of the SOFCs stack is examined in relation to direct load, electric networks, and superconducting magnetic energy storage devices (SMES) for additional validation and illustration. The role of the SOFCs stack in controlling the active and reactive power delivered to the network and direct load is investigated using two controllers: one to control the inverter, which converts the SOFC’s dc output to the main network, and the other to control the SMES. The Simulink/MATLAB environment is used to indicate the validity of the proposed framework under both steady-state and dynamical conditions. The comprehensive assessments show that the CDO capabilities are very effective when used with microgrids.
The daily relations of co-rumination and perseverative cognition
Authors: Kovács Lilla Nóra , Kocsel Natália , Tóth Zsófia , Smahajcsik-Szabó Tamás , Karsai Szilvia , Kökönyei Gyöngyi 
Published: January 2025

Abstract
Emotion regulation plays a crucial role in responding to daily stressors. While intrapersonal emotion regulation has received significant attention, the role of interpersonal emotion regulation remains understudied. This study aimed to explore the associations between intrapersonal perseverative cognition (i.e., rumination and worry) and interpersonal perseverative cognition (i.e., co-rumination), in the context of daily negative events. A daily diary study was conducted with a sample of university students (N = 178). The study comprised a baseline survey assessing trait-level variables, followed by a diary study where participants were sent an approximately two-minute-long survey every evening for 10 consecutive days. The findings indicated that daily co-rumination was associated with daily intrapersonal perseverative cognition, even when controlling for trait-level worry. The association between intra- and interpersonal perseverative cognition was stronger in the presence of daily negative events. Our findings indicate a need for further investigation into the contemporaneous relationship between co-rumination and intrapersonal rumination/worry, to ascertain their unique or joint adverse effects on mental health, especially within the context of daily negative events.
Mobility of South America’s transcontinental drainage divide and shrinkage of the Paraná river basin linked to lithologic and geodynamic controls
Authors: Crelier Caio , Zumba Adriana , Peifer Daniel , Val Pedro 
Published: January 2025

Abstract
The South American Transcontinental Drainage Divide (TDD) is roughly oriented NE-SW from its Atlantic termination in SE Brazil to its NW termination in the limits between the southwestern edges of the Amazon Craton and the Andean Foreland. Based on a weak spatial coincidence with 90 − 70 Ma igneous intrusions, the origin and stability of the TDD have been attributed to intraplate magmatic activity. Using geomorphic analysis of drainage divides, we infer an overall southward migration of the TDD, even in areas with constrained uplift associated with intrusions. Most of the TDD is also the main drainage divide of the Paraná River Basin (PRB). Our analysis of the PRB divides reveals wholesale basin shrinkage, consistent with the southward migration of the TDD. We infer that the basalts of the Serra Geral Group induce topographic inversion of the PRB. Additionally, the PRB’s thicker continental crust contributes to its higher elevation. Non-uniform dynamic subsidence rates since 50 Ma likely lowered relief in the basin headwaters near the TDD and enhanced across-divide relief asymmetry. Together, these internal and external factors keep the PRB elevated, with low relief, and make it topographically vulnerable to long-term drainage area loss. These base-level controls explain the position and mobility of the TDD.
Factors Associated With Social-Pragmatic Understanding in Deaf and Hard of Hearing and Typically Hearing 6-Year-Old Children
Authors: Tuohimaa Krista , Loukusa Soile , Löppönen Heikki , Aarnisalo Antti A. , Dietz Aarno , Hyvärinen Antti , Laitakari Jaakko , Rimmanen Satu , Salonen Jaakko , Sivonen Ville , Tennilä Tanja , Tsupari Teija , Vikman Sari , Virokannas Nonna , Hautala Johanna , Tolonen Anna-Kaisa , Välimaa Taina , Kunnari Sari 
Published: January 2025

Purpose:
Children develop social-pragmatic understanding with the help of sensory, cognitive, and linguistic functions by interacting with other people. This study aimed to explore (a) associations between auditory, demographic, cognitive, and linguistic factors and social-pragmatic understanding in children who use bilateral hearing aids (BiHAs) or bilateral cochlear implants (BiCIs) and in typically hearing (TH) children and (b) the effect of the group (BiHA, BiCI, TH) on social-pragmatic understanding when the effects of demographic, cognitive, and linguistic factors are controlled for.

Method:
The Pragma test was used to assess social-pragmatic understanding in 119 six-year-old children: 25 children who use BiHAs, 29 who use BiCIs, and 65 TH children. The Pragma test is a standardized test that requires answering socially and contextually demanding questions with varying focuses and thus enables a comprehensive assessment of social-pragmatic understanding. Associations between auditory, demographic, cognitive, and linguistic factors and the Pragma test performance were analyzed. Between-groups differences in the Pragma test performance were analyzed while statistically controlling for the effects of factors that were associated with the Pragma test scores.

Results:
The BiHA users who had better unaided pure-tone average hearing thresholds had better social-pragmatic understanding. A higher level of maternal education and nonverbal intelligence were associated with a better social-pragmatic understanding in the BiHA and BiCI groups. Linguistic abilities correlated strongly with social-pragmatic understanding in all groups. The deaf and hard of hearing (DHH) groups differed from the TH children in social-pragmatic understanding even after the effects of maternal education, nonverbal intelligence, and linguistic skills were controlled for.

Conclusions:
Despite early diagnosis of hearing loss and intervention, many DHH children are still at risk for social-pragmatic difficulties. Several factors may associate with social-pragmatic understanding in DHH children, which highlights the importance of a wide-ranging, multidisciplinary assessment, and the planning of focused, assessment-based interventions.
Fast and low‐cost determination of prostate‐specific antigen using paper‐based immunodevice modified with Cu@CuS@Au NPs nanocages
Authors: Duan Yi , Wu Qi , Lin Jiangtao , Duan Yourong , Wang Qi , Li Yuanyuan 
Published: January 2025

AbstractIn this study, the authors designed a paper‐based electrochemical immunodevice modified with copper embedded in copper sulphide hollow nanocages wrapped with Au nanoparticles (Cu@CuS@Au NPs) for the specific detection of prostate‐specific antigen (PSA), aiming to advance point‐of‐care testing. The large specific surface area of Cu@CuS nanocages enables efficient capture of biotin antibodies, leading to the direct amplification of the signal through the inhibition of electron transport in the redox process of Cu, eliminating the need for universal redox electron mediators. Additionally, Au NPs on the surface of Cu@CuS can accelerate charge transfer and conjugate with anti‐PSA. The hierarchical morphology and structure of Cu@CuS nanocages were characterised using scanning electron microscopy and transmission electron microscopy. The fabrication process of the immunodevice was monitored using cyclic voltammetry and electrochemical impedance spectroscopy analyses. PSA was sensitively detected using differential pulse voltammetry on this proposed immunodevice within a linear range from 0 to 100 ng/ml (R2 = 0.996), achieving a low detection limit of 0.077 ng/ml. In addition, the practicality of the developed immunosensor has been proven by successfully detecting PSA in human serum samples obtained from clinical settings. The integration of electrochemical sensors and microfluidic devices holds promise for developing cost‐effective approaches in clinical immunoassays.
La conservation hermétique des céréales : comment les avancées de la recherche ont été ignorées au XXe siècle en France
Authors: Fleurat-Lessard Francis 
Published: January 2025

Pendant la première moitié du XXe siècle, le stockage des grains à la ferme s’est effectué essentiellement en sacs conservés en grenier. À partir des années 1930 sont apparus les premiers silos de stockage de type coopératif. Ces structures de conservation en vrac ont rapidement été confrontées à des difficultés de préservation de la qualité en cas de conservation à long terme. Il en a découlé un besoin de recherche, notamment pour comprendre la dynamique de prolifération des moisissures de stockage et de multiplication des insectes dans ces structures de grand volume. Après la deuxième guerre mondiale et la découverte des premiers insecticides organiques de synthèse, leur utilisation s’est généralisée. À partir de 1960, pour faire face aux nombreuses avaries dues à l’échauffement des grains stockés trop humides, des laboratoires spécialisés dans l’étude des facteurs de détérioration des grains stockés ont été constitués dans tous les pays développés producteurs de céréales, avec un même objectif : améliorer la connaissance sur le fonctionnement dynamique de l’écosystème du stock de céréales. À partir des années 1970, ces groupements de chercheurs se sont organisés en équipes pluridisciplinaires venant présenter régulièrement leurs résultats dans des conférences internationales. Dès 1980, ils se sont intéressés à la conservation des grains en atmosphère modifiée ou sous inertage, en substitution aux traitements insecticides et pour limiter les risques liés aux moisissures à mycotoxines. En France, les acquis de la recherche sur le stockage hermétique ont été ignorés pour plusieurs raisons : i) le manque de relais efficace entre recherche et application des innovations, notamment pour les producteurs-stockeurs en agriculture biologique ; ii) le verrouillage par le secteur commercial de l’option consistant à remplacer les traitements correctifs des avaries par une démarche écosystémique de protection antiparasitaire intégrée ; iii) l’absence de valorisation des systèmes experts permettant une meilleure maîtrise des dérives de qualité pendant la conservation.
The relationship between soil quality and crop productivity across three tillage systems in south central Honduras
Authors: Stine Melissa A. , Weil Ray R. 
Published: January 2025

AbstractAgricultural soil quality (SQ) research has focused on the influence of individual soil properties, particularly those related to soil organic matter (SOM), on soil processes such as water movement and retention, and nutrient cycling. However, few studies have assessed the influence of these SOM-related properties on crop productivity, particularly under different management systems. Furthermore, agricultural SQ research in tropical regions is limited. The objectives of this study were, for a tropical site, to determine (1) the relationship between selected soil characteristics and resulting soil functional properties such as aggregate stability and porosity, and (2) the relationship between SQ indicators and productivity of corn under three different tillage systems. Twelve plots were randomly demarcated in three adjacent fields under different tillage systems. Profiles were described by augering and the surface soil (0–7.5 cm) was sampled just prior to crop harvest and analyzed for texture, standard soil tests (pH, available P, K, Mg and Ca) and SQ parameters including total N, total and active fraction C, porosity and aggregate stability. Corn dry grain m−2, above-ground crop dry matter m−2, and dry grain per plant was measured in each plot. Soil C parameters were highly predictive of macroaggregate stability and soil porosity across different tillage systems. Macroaggregate stability and soil C (particularly active C oxidizable by 0.025m KMnO4) were highly correlated with crop productivity across tillage systems. These findings suggest that soil C in the surface layer, especially the active C fraction, markedly affected the productivity of this tropical soil through its influence on soil structure.
The ecological economics of insecticide use associated with the Maine potato industry, based on a producer survey
Authors: Ziegler C.R. , Donahue D.W. , Drummond F.A. , Smith S.N. 
Published: January 2025

AbstractEnvironmental risks and costs associated with insecticide applications in Maine potato farming have been characterized and estimated using an Aroostook producer survey. The results of this study allow for comparison of pest management strategies based on producer concern for various aspects of the environment, including water quality, human health and animal safety. ‘Willingness to pay’ survey methodology was used to acquire an environmental cost range for insecticide use. Maine potato producers are willing to pay between $1.00 acre−1 (one acre = 0.4047ha) and $26.80acre−1 to avoid or reduce environmental risk associated with a single insecticide application. The Aroostook producer survey indicates that producers with small farms are generally willing to pay more to avoid or reduce environmental risk than producers with large farms. Mean Maine potato farm size has more than tripled over the past 40 years; therefore, concern for environmental quality on Maine potato farms could be decreasing.
Moral confidence in agriculture
Authors: Zimdahl Robert L. 
Published: January 2025

AbstractThose engaged in agriculture possess a definite but unexamined moral confidence or certainty about the correctness of what they do. This paper examines the origins of that confidence and questions its continued validity. The paper argues that those engaged in agriculture are morally confident about the rightness of their activity. The basis of the moral confidence is not obvious to those who possess it or to the public. In fact, the moral confidence that pervades agricultural practice is potentially harmful because it is unexamined. Suggestions for expanding agriculture's moral scope are made to approach the question of where moral values originate, and what are, or ought to be, the moral standards for agriculture in our post-industrial, information-age society. Part of expanding agriculture's moral scope will be giving up some of our hubris about the goodness of our culture and its agriculture. The paper advocates analysis of what it is about our agriculture and our society that thwarts or limits our aspirations for agriculture and needs modification. We must strive to nourish and strengthen those features of our agriculture that are beneficial and change those that are not. To fit ourselves to this task, we must be sufficiently confident to study ourselves and our institutions and dedicated to the task of modifying both.
The wholesale produce auction: An alternative marketing strategy for small farms
Authors: Tubene Stephan , Hanson James 
Published: January 2025

AbstractSmall farmers urgently need alternative marketing strategies if they are to achieve the goals of a more sustainable agriculture. This study was a survey of nine Pennsylvania wholesale produce auctions, all established between 1984 and 1998. The main goals of the auctions were to serve local communities, provide high-quality produce to local consumers and make profits. The results showed that the auctions typically employ an average of 7–10 people per growing season. The five most common commodities sold were cantaloupe, watermelon, tomato, pumpkin and sweet corn. Asparagus and onions were the least sold during a regular growing season. In terms of market share, roadside market operators purchased the largest proportion of produce (40%), followed by farmers (27%), chain food stores (16%), independent grocery stores (11%) and restaurants (6%), making up an average annual gross sale of $3.5 million per auction. The study revealed that the nine Pennsylvania produce auctions were successful in meeting their goals. The reasons for success included private ownership, excellent quality and freshness of produce, good location, local produce recognition, clientele availability and customer-oriented business. The auction managers identified some weaknesses, including inconsistent and poor grading, limited space in the auction facility, produce unavailability and limited volume, lack of cooling facility, price fluctuation and slow service. The study indicated that local wholesale produce auctions are a useful marketing alternative for small farmers in Pennsylvania, by providing marketing outlets and convenient shopping centers for sellers and buyers; by securing a source of fresh and locally grown produce not found in traditional wholesale terminal markets; and by allowing exchange and networking among farmers and buyers. Consequently, the wholesale produce auction can be a useful model for an alternative marketing strategy and can provide considerable benefits to small farm and rural communities.
Killing cover crops mechanically: Review of recent literature and assessment of new research results
Authors: Creamer Nancy G. , Dabney Seth M. 
Published: January 2025

AbstractCover-crop residues left on the soil surface as a mulch in no-till crop production systems protect the soil from erosion, increase water infiltration and suppress weeds. Because many growers using cover crops want to reduce chemical inputs, non-chemical methods of killing or suppressing cover crops are needed. In the first part of this paper we review the current literature and discuss advantages and disadvantages of five mechanical methods for killing cover crops, i.e., mowing, rolling, roll-chopping, undercutting and partial rototilling. We also report on three new studies that broaden the current literature, including planting into freshly killed residue. In the first study, the use of planter attachments to remove surface residues from the planter row improved stands when cotton was no-till planted 2–7 days after mowing cover crops in Mississippi. In the second study, 100% of a rye/vetch cover crop in Missouri was killed by mowing, and greater than 90% was killed by roll-chopping. Cotton stands were reduced by the use of row cleaners that clogged when the cover crop was roll-chopped or mowed on the same day that the crop was planted. The third study evaluated three methods of mechanically killing summer cover crops in North Carolina. Undercutting provided greater than 95% kill for five of six broadleaf species, and two of five grass species. Mowing effectively killed all six broadleaf cover crops, but re-growth occurred with three of five grasses, with the exception of nearly mature German foxtail millet and mature Japanese millet. In general, rolling did not effectively kill broadleaf or grass cover crops, with the exception of nearly mature German foxtail millet, mature Japanese millet and mature buckwheat.
Barriers to the adoption of organic farming by cash-crop producers in Austria
Authors: Schneeberger Walter , Darnhofer Ika , Eder Michael 
Published: January 2025

AbstractMost of Austria's organic farms are located in mountainous areas and produce mainly milk and beef, whereas a low percentage of cash-crop farms are organic. As a result, the demand for organically grown crops cannot be fully met by domestic production. This paper investigates the barriers to adoption of organic agriculture by cash-crop farmers in Austria. In a written survey, 383 conventional farmers provided information on their perception of organic farming and rated the importance of potential barriers to adoption. Technical challenges in cropping and additional labor requirements were rated as the most important barriers, followed by fear of decreased income and marketing problems. Structural and social issues, such as farm size and lack of support from family members, were less important.