bluuebunny/crossref_metadata_2025_split huggingface dataset:
A AMAZÔNIA NO CONTEXTO DA GEOGRAFIA ESCOLAR, UMA DISCUSSÃO NECESSÁRIA A PARTIR DA PERCEPÇÃO DISCENTE
Authors: Maria da Silva Renata 
Published: January 2024

Ao longo de décadas a Amazônia enfrenta formas variadas de saques, violações e espoliação, fenômenos que se intensificaram com o avanço do capital. Mediante a lógica capitalista, é vista apenas como recurso e fonte inesgotável de riquezas. Suas particularidades e sua sociobiodiversidade são desconsideradas e, muitas vezes, negadas. Esta pesquisa se caracteriza como exploratória, com abordagem quali-quantitativa, os procedimentos utilizados consistiram em levantamento e análise bibliográfica, documental e pesquisa de campo. O objetivo deste estudo é analisar como os estudantes do 8º do Ensino Fundamental de uma escola pública, localizada no norte do estado de Mato Grosso, percebem a Amazônia no contexto da Geografia Escolar. Parte dos resultados revelou que a maioria dos participantes identifica a Amazônia como uma “floresta”, habitada apenas por “índios e animais”, cuja manutenção é muito importante para a produção de oxigênio. Sobre a localização, muitos discentes responderam que a Amazônia fica no estado do Amazonas, portanto, não se identificam como amazônida. A situação é preocupante, pois esses alunos desconhecem a temática proposta e ocorre uma negativa voluntária ou involuntária da Amazônia em suas vidas, fato que pode colaborar para o descaso, a desconsideração e o desinteresse da sociedade no tocante à realidade socioambiental da maior floresta tropical do planeta.
NOVAS TERRITORIALIZAÇÕES DA FRONTEIRA ECONÔMICA AMAZÔNICA: O EIXO DA TRANSAMAZÔNICA DE LÁBREA A APUÍ – O AGRONEGÓCIO, CONFLITOS E IMPACTOS AMBIENTAIS
Authors: Da Silva Silvio Simione , Maciel de Araújo João , Vieira da Silva Rita Clara 
Published: January 2024

Este artigo traz um estudo sobre as novas feições territoriais da fronteira econômica da Amazônia Sul-ocidental, tendo como base a questão da expansão do agronegócio de grãos e seus impactos sobre outras formas de produção existentes, tal como sobre a floresta. Teve-se como recorte espacial o eixo da Rodovia Transamazônica entre as cidades de Lábrea e Apuí, no estado do Amazonas. Para isto desenvolveu-se estudos baseados em referenciais bibliográficas, relatórios técnicos, informações jornalísticas sobre a região e, uma atividade de campo, na região referida, com observações sistemáticas in loco. Pode-se então, entender que a expansão do agronegócio na região reflete aspectos da reterritorialização do capitalismo agrário, na atualidade, da sua rearticulação na Amazônia Sul-ocidental. Neste processo, o que ocorre na região de estudo reflete a recolocação espacial de agentes da fronteira provindos desde o Mato Grosso, passando por Rondônia Acre e sul do estado do Amazonas, onde o agronegócio de grãos se expande por terras que antes estavam ocupadas por atividades como a pecuária bovina e agricultura familiar. Por consequência desta perda de espaço para estas atividades, esses criadores de gado e agricultores familiares passaram a procurar outras áreas, sobretudo, mais distantes e/ou florestais que oferecem grandes possibilidades de serem incorporadas ao espaço produtivo, mesmo que necessite de desmatamento para a prática de atividades agropecuárias e agricultura de subsistência. Em tudo isto, viu-se ocorrer grandes expressões dos impactos ambientais, sociais e econômicos, junto ao aumento de indícios de conflitos sociais, no conjunto desta nova territorialização do capitalismo agrário na região.
Spatially organized tumor-stroma boundary determines the efficacy of immunotherapy in colorectal cancer patients
Authors: Zhang Rongxin , Feng Yu , Ma Wenjuan , Zang Yupeng , Guo Yanying , Li Young , Zhang Yixuan , Dong Xuan , Liu Yi , Zhan Xiaojuan , Pan Zhi-Zhong , mei Luo , Wu Miaoqing , Chen Ao , Chen Gong , Liu Longqi , Zhou Jingying 
Published: January 2024

Abstract

Colorectal cancer (CRC) patients with mismatch repair (MMR)-deficient (dMMR) but not MMR-proficient (pMMR) tend to benefit from immune checkpoint blockade (ICB) therapy. To uncover the rules governing these varied therapeutic responses, we integrated spatial enhanced resolution omics-sequencing (Stereo-seq), single-cell RNA sequencing, and multiplexed imaging analysis to create high-definition spatial maps of tumors from treatment-naïve and ICB-treated CRC patients. Our results identified the spatial organization and immune status of the tumor-stroma boundary as a distinctive feature of dMMR and pMMR CRCs, which determines ICB response. The physical interactions and abundance of LAMP3+ DCs and CXCL13+ T cells shape the ICB-responsive tumor-stroma boundary, whereas CXCL14+ cancer-associated fibroblasts remodel extracellular matrix to form a structural barrier in non-responders. Our work therefore uncovered the black box of how the molecular and cellular spatial structures of tumors determine ICB response, raising the possibility of reprogramming tumor-stroma boundary for sensitizing immunotherapies in the majority of CRCs.
Exploring Cellular Gateways: Unraveling the Secrets of Disordered Proteins within Live Nuclear Pores
Authors: Yang Weidong , yu wenlan , Tingey Mark , kelich joseph , Li Yichen , yu jinjie , Junod Samuel , jiang zecheng , Hansen Ian , Guessaymi Nacef 
Published: January 2024

Abstract

Understanding the spatial organization of nucleoporins (Nups) with intrinsically disordered domains within the nuclear pore complex (NPC) is crucial for deciphering eukaryotic nucleocytoplasmic transport. Leveraging high-speed 2D single-molecule tracking and virtual 3D super-resolution microscopy in live HeLa cells, we investigated the spatial distribution of all eleven phenylalanine-glycine (FG)-rich Nups within individual NPCs. Our study reveals a nuanced landscape of FG-Nup conformations and arrangements. Five FG-Nups are steadfastly anchored at the NPC scaffold, collectively shaping a central doughnut-shaped channel, while six others exhibit heightened flexibility, extending towards the cytoplasmic and nucleoplasmic regions. Intriguingly, Nup214 and Nup153 contribute to cap-like structures that dynamically alternate between open and closed states along the nucleocytoplasmic transport axis, impacting the cytoplasmic and nuclear sides, respectively. Furthermore, Nup98, concentrated at the scaffold region, extends throughout the entire NPC while overlapping with other FG-Nups. Together, these eleven FG-Nups compose a versatile, capped trichoid channel spanning approximately 270 nm across the nuclear envelope. This adaptable trichoid channel facilitates a spectrum of pathways for passive diffusion and facilitated nucleocytoplasmic transport. Our comprehensive mapping of FG-Nup organization within live NPCs offers a unifying mechanism accommodating multiple transport pathways, thereby advancing our understanding of cellular transport processes.
Fellow Eye Data for IOL Calculation in Eyes Undergoing Combined Phacovitrectomy
Authors: Kohnen Thomas , Kaiser Klemens , Bucur Julian , Jandeworth Tyll , Lwowski Christoph 
Published: January 2024

Abstract
Purpose To evaluate whether the intraocular lens (IOL) calculation of the fellow eye (FE) can be used in eyes undergoing combined phacovitrectomy.Methods In this retrospective, consecutive case series, we enrolled patients who underwent silicone oil (SO) removal combined with phacoemulsification and IOL implantation at the Department of Ophthalmology, Goethe University, Frankfurt, Germany. Preoperative examinations included biometry using the IOL Master 700 (Carl Zeiss Meditec AG, Jena, Germany). We used the IOL calculation of the FE (FE group) to calculate the prediction error compared with the IOL calculation using only the axial length of the FE (AL-FE group), as well as using the axial length (AL) of the operated eye (OE group) in addition to the measurable biometric parameters. For the IOL calculation, the Barrett Universal II formula was used. We compared the mean prediction error (MPE) as well as the mean (MAE) and median absolute prediction error (MedAE) with each other. In addition, the number of eyes with ± 0.50, ± 1.00, and ± 2.00 diopters (D) deviation from the target refraction was compared.Results In total, 79 eyes of 79 patients met our inclusion criteria. MedAE was lowest in the OE group (0.41 D), followed by FE group (1.00 D) and AL-FE group (1.02 D). Comparison between the AL-FE and FE groups showed no statistically significant difference (p = 0.712). Comparing eyes within ± 0.50 D of the target refraction, the OE group (50 eyes, 63.3%) performed best, followed by the AL-FE group (22 eyes, 27.8%) and the FE group (21 eyes, 26.6%).Conclusion Our results indicate no clinically relevant difference between using the IOL calculation of the fellow eye versus using only the axial length of the fellow eye in addition to the measurable parameters for the IOL calculation. A two-step procedure should always be strived for.
Mucilage effects on the soil water characteristics curve, mechanical consistency, and penetration resistance of compacted heavy and light texture soils.
Authors: Fathinejad Asma , Mohammadi Mohammad Hossein , Etesami Hassan , Zarebanadkouki Mohsen 
Published: January 2024

Abstract
Aims
This study aimed to investigate the effects of mucilage on soil mechanical properties and the soil water characteristic curve(SWC).
Methods
Six soil samples with a wide range of physical and chemical properties were taken and then SWC, soil penetration resistance(SR), and the Atterberg limits were measured at compaction pressures of 100, 200, 300, 400, and 600 kPa, and mucilage(extracted from chia seed)concentrations of 0, 0.1%, 0.3%, and 0.5%. Based on their texture and consistency, the studied soils were divided into two groups: sandy to loamy and clayey soils.
Results
Results showed that the effect of mucilage, especially at 0.1%, on the Atterberg limits was more significant for clayey soils. The mucilage reduces the compaction potential of light-texture soils and improves penetrability, especially when the soil is dry and under a high compaction pressure. This results in more water retention, especially at low matric suction. Mucilage causes fine-texture soils to be more prone to compaction. The inconsistent effects of mucilage on heavy and light-texture soils may be due to mucilage arrangement and concentration of the solution within fine and coarse pores.
Conclusion
It is concluded that the increase in water retention would be significant as long as the compressibility is not amplified due to the presence of the mucilage, especially under high compaction energy. The mucilage can improve water retention and penetrability, but tillage or any disturbance should be conducted with care for intensively cultivated soils, which may have higher root exudate concentration and compressibility than bare and fallow soils.
Inherited defects of piRNA biogenesis cause transposon de-repression, impaired spermatogenesis, and human male infertility
Authors: Stallmeyer Birgit , Bühlmann Clara , Stakaitis Rytis , Dicke Ann-Kristin , Ghieh Farah , Meier Luisa , Zoch Ansgar , MacLeod David MacKenzie , Steingröver Johanna , Okutman Özlem , Fietz Daniela , Pilatz Adrian , Escamilla Antoni Riera , Xavier Miguel , Ruckert Christian , Persio Sara Di , Neuhaus Nina , Gurbuz Ali Sami , Şalvarci Ahmend , May Nicolas Le , McEleny Kevin , Friedrich Corinna , Heijden Godfried van der , Wyrwoll Margot J. , Kliesch Sabine , Veltman Joris A. , Krausz Csilla , Viville Stéphane , Conrad Donald , O'Carroll Donal , Tüttelmann Frank 
Published: January 2024

Abstract

Piwi-interacting RNAs (piRNAs) are crucial for transposon silencing, germ cell maturation, and fertility in male mice. Here, we report on the genetic landscape of piRNA dysfunction in humans and present 39 infertile men carrying biallelic variants in 14 different piRNA pathway genes, including PIWIL1, GTSF1, GPAT2, MAEL, TDRD1, and DDX4 as novel disease genes. The testicular phenotypes repeatedly differ from those of the respective knockout mice and range from complete germ cell loss to the production of a few morphologically abnormal spermatozoa. LINE1 expression in spermatogonia links impaired piRNA biogenesis to transposon de-silencing and serves to classify variants as functionally relevant. Furthermore, an abolished expression of not only the encoded proteins but also of additional piRNA factors reveals co-dependencies within the human pathway. These results establish the disrupted piRNA pathway as a major cause of human spermatogenic failure and provide insights into transposon silencing in human male germ cells.
Predicting Carbon Dioxide Emissions in the United States of America Using Machine Learning Algorithms
Authors: Chukwunonso Bosah Philip , Al-wesabi Ibrahim , Shixiang Li , AlSharabi Khalil , Al-Shamma’a Abdullrahman A. A. , Farh Hassan M. Hussein , Saeed Fahman , Kandil Tarek , Al-Shaalan Abdullah M. 
Published: January 2024

Abstract

In the past few decades, several nations have experienced air pollution and environmental difficulties because of carbon dioxide (CO2) emissions. One of the most crucial methods for regulating and maximizing CO2 emission reductions is precise forecasting. Four machine learning algorithms with high forecasting precision and low data requirements were developed in this study to estimate CO2 emissions in the United States (US). Data from a dataset covering the years 1973/01 to 2022/07 that included information on different energy sources that had an impact on CO2 emissions were examined. Then, four algorithms performed the CO2 emissions forecast from the layer recurrent neural network with 10 nodes (L-RNN), a feed-forward neural network with 10 nodes (FFNN), a convolutional neural network with two layers with 10 and 5 filters (CNN1), and convolutional neural network with two layers and with 50 and 25 filters (CNN2) models. Each algorithm's forecast accuracy was assessed using eight indicators. The three preprocessing techniques used are (1) Without any processing techniques, (2) Processed using max-min normalization technique, and (3) Processed using max-min normalization technique and decomposed by variation mode decomposition (VMD) technique with 7 intrinsic mode functions and 1000 iterations. The latter with L-RNN algorithm gave a high accuracy between the forecasting and actual values. The results of CO2 emissions from 2011/05 to 2022/07 have been forecasted, and the L-RNN algorithm had the highest forecast accuracy. The research makes significant literary contributions. One of the first studies to focus on predicting CO2 emissions in the USA using a combination of three preprocessing approaches and four machine-learning algorithms, predicting the number of overall CO2 emissions while also accounting for a broader range of inputs.
Exerting pulling forces in fluids by directional disassembly of microcrystalline fibres
Authors: Kudernac Tibor , Pantaleone Luis , Calicchia Eleonora , Martinelli Jacopo , Stuart Marc , Browne Wesley , Portale Giuseppe , Tych Katarzyna (Kasia) 
Published: January 2024

Abstract

Bio-molecular polymerization motors are molecular systems that use supramolecular (de-)polymerization to mediate the conversion of chemical potential into useful mechanical work. Microcrystallization is ideally suited to reverse-engineer this strategy in artificial systems since self-organization of molecules in a crystal lattice resembles the process of polymer growth/disassembly but with increased predictability of molecular events in a highly ordered microscopic structure. With the intent to explore new chemo-mechanical transduction strategies, we developed a synthetic mechanically-active molecular system that can generate forces via controlled disassembly while being freely suspended in fluid. The amphiphilic monomer self-assembles into rigid, high-aspect ratio microcrystalline fibres with assembly regulated by a coumarin-based pH switching motif. In the structures formed, the crystal morphology determines the monomer reactivity at the interface, resulting in anisotropic etching of the fibres. This effect is used to exert directional pulling forces on microscopic beads adsorbed on the crystal surface through weak multivalent interactions. Mechanistic insights were provided by optical tweezers force spectroscopy, which quantified a stall force of 2.3 pN (+/- 0.1 pN) exerted by this ratcheting mechanism and the mechanical work produced by the disassembly of the fibres.
Municipal solid waste composting with amendments reduces heavy metals hazard, ecological risks and greenhouse gases emission
Authors: Devi Manisha , Chaudhry Smita , Yadav Rajender Kumar , Yadav Gajender 
Published: January 2024

Abstract
Open dumping and landfilling of municipal solid waste (MSW) in India cause environmental hazards and greenhouse gases (GHGs) emissions. Therefore, studies require to assess the potential of aerobic composting of MSW to minimize these risks. This study evaluates the potential of MSW composting with or without some amendments (i.e. G-gypsum, ES-elemental sulphur and RS-rice straw) in minimizing these risks. The composition, physicochemical characteristics including heavy metals (HMs), the potential pollution hazards and GHGs emissions were assessed for the MSW collected from domestic and industrial sites of Haryana, India. Industrial MSW had higher pH (7.31) and HMs concentrations (e.g. Cr, Cd, Cu, Ni, Pb and Zn), than domestic MSW. HMs were found within the critical limits for both sites except Cr and Cu in industrial MSW. Composting lowered the HMs and ecological risk and increased N, P, K and organic carbon. Co-composting with amendments like gypsum, elemental sulphur and rice straw improved C:N ratio, nutrients concentration and accelerated the degradation process. The pollution indices enrichment factor, contamination factor, geoaccumulation index, ecological risk and potential ecological risk index indicated lower ecological risk and HMs concentration in domestic site MSW. On-site GHGs emission significantly differed in space and seasons. Emission of CO2, CH4 and N2O from domestic landfill site were 20%, 27% and 64% higher than industrial and higher in monsoon season, followed by summer and winter, respectively. The highest GHGs emission was from center (heap top) (924.58 mg m-2 h-1), followed by middle (805.23 mg m-2 h-1) and peripheral (717 mg m-2 h-1) area of sites. Use of ES and RS amendments in co-composting reduced up to 41% GHGs emission than landfill emissions. MSW co-composting with elemental sulphur and rice straw can be a potentially safer alternative for the management of both domestic and industrial MSW than open landfill dumping.
Orally Available Small Molecule Regulates TXNIP Expression and Glucagon Action for the Treatment of Diabetes
Authors: Wang Xiaolei , Luo Zhikuan , Han Jun , Tian Qiong , Jing Ruijun , Dong Ao , Li Lingzhi , Jia Hongbin , Lv Tinghong , Dang Yunkun , Lai Fan , Chen Shili , Ma XuSheng , Zheng Haixue 
Published: January 2024

Abstract
In the progression of diabetes, sustained glucagon secretion facilitates glucose production. Despite its significance, no treatments currently target this vital parameter. We employed the promoter sequence of thioredoxin-interacting protein (TXNIP) as a screening target, evaluated our in-house chemical library, and subsequently undertook high-throughput screening and optimization via various medicinal chemistry techniques. Our efforts led to the identification of SKL-1223, an orally administered, non-toxic small molecule capable of effectively regulating blood glucose levels in both streptozotocin-induced and obesity-induced (db/db) diabetic mice. Furthermore, our study elucidated the hypoglycemic effect of glucagon and its role in reducing hepatic glucose output. On a mechanistic level, SKL-1223 interacts with the E-box region of the TXNIP promoter, consequently suppressing TXNIP transcription and associated signaling pathways. Thus, our study introduces a novel compound, offering a fresh therapeutic avenue for diabetes beyond existing regimens.
Salicylic and jasmonic acid attenuate the salt stress effects in lettuce
Authors: Neto Hozano de Souza Lemos , Neto Hozano de Souza Lemos , Garita Sebastián Andrés , Bernardo Valeria Fernanda , Ruscitti Marcela Fabiana , Arango Maria Cecilia , Wahnan Laura Karina , Guimarães Marcelo de Almeida 
Published: January 2024

Abstract
Salt stress reduces growth and change physiological and biochemical responses of plants. Salicylic acid (SA) and jasmonic acid (JA) are plant hormones involved in plant defense metabolism against abiotic stress. Therefore, the objective was to evaluate whether the foliar application of SA and JA, isolated or combined, attenuate the salt stress effects, as well as its possible effects on the physiological, biochemical and growth responses of lettuce plants. The plants were arranged in a completely randomized design, factorial scheme, with three salinity levels (1.65; 3.65 and 7.65 dS m− 1) and foliar application of SA (0 and 500 µM) and JA (0 and 100 µM). Photosynthesis, transpiration, stomatal conductance, internal carbon concentration, chlorophyll content, malondialdehyde (MDA) content, proline, relative membrane conductivity, soluble proteins, total and reducing sugars and phenols of lettuce leaves and roots were determined. Under the highest salinity level, shoot and root fresh weight reduced by 21.84% and 13.80%. Gas exchange also reduced by salinity, except for net photosynthesis. The combined application (SA + JA) increased the total soluble sugar content in leaves by 74.23%. Under moderate salinity, application of these growth regulators isolated induced the highest total sugar content in leaves. Proline content in leaves was higher under the highest salinity level and without application of growth regulators. However, with SA application, leaf proline content was reduced by 37.92%, while root proline increased with SA application under the highest salinity level. Exogenous application of salicylic acid and jasmonic acid improve salt stress tolerance in lettuce plants, although jasmonic acid is more effective than salicylic acid.
Diffuse Idiopathic Skeletal Hyperostosis: Prevalence and Risk Factors
Authors: Madariaga Maria Cecilia , Groff Hannah , O’Malley Nicholas A. , Thompson Jeffery , Apazidis Alexios 
Published: January 2024

Abstract
Background
Diffuse Idiopathic Skeletal Hyperostosis (DISH) is a degenerative disease of the spine which has been estimated to affect anywhere from 8–37% of the population. In addition, previous literature has shown an association between DISH and serious disability including dysphagia. Previous research detailing prevalence and risk factors for DISH, however, have been limited by studying homogenous populations.
Methods
Patients who received CT scans of the spine during hospital admission were included. Demographic data including age, sex, race/ethnicity, smoking history, alcohol use, and comorbidities were collected via chart review. DISH was defined as the presence of flowing ossifications along ≧ 4 contiguous vertebral bodies on CT. The location, number of levels, and side of ossification were also collected. Results were analyzed using t-test and chi-square analyses. Significance was set to p < 0.05
Results
1097 patients with CT scans were identified, revealing a DISH prevalence of 20.3%. Patients with DISH were significantly older than those without DISH and had significantly higher CCI scores when compared to the non-DISH cohort. Significant differences were also observed between ethnic groups. Men were found to have a significantly higher prevalence of DISH when compared to women. Finally, those with DM, PVD, Dementia, COPD, and CHF were noted to have higher prevalence of DISH when compared to those without DISH.