bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of neoepitope reactive T-cell receptors guided by HLA-A*03:01 and HLA-A*11:01 immunopeptidomics
Authors: Ade Catherine M , Sporn Matthew J , Das Sudipto , Yu Zhiya , Hanada Ken-ichi , Qi Yue A , Maity Tapan , Zhang Xu , Guha Udayan , Andresson Thorkell , Yang James C 
Published: September 2023

BackgroundTumor-specific mutated proteins can create immunogenic non-self, mutation-containing ‘neoepitopes’ that are attractive targets for adoptive T-cell therapies. To avoid the complexity of defining patient-specific, private neoepitopes, there has been major interest in targeting common shared mutations in driver genes using off-the-shelf T-cell receptors (TCRs) engineered into autologous lymphocytes. However, identifying the precise naturally processed neoepitopes to pursue is a complex and challenging process. One method to definitively demonstrate whether an epitope is presented at the cell surface is to elute peptides bound to a specific major histocompatibility complex (MHC) allele and analyze them by mass spectrometry (MS). These MS data can then be prospectively applied to isolate TCRs specific to the neoepitope.MethodsWe created mono-allelic cell lines expressing one class I HLA allele and one common mutated oncogene in order to eliminate HLA deconvolution requirements and increase the signal of recovered peptides. MHC-bound peptides on the surface of these cell lines were immunoprecipitated, purified, and analyzed using liquid chromatography-tandem mass spectrometry, producing a list of mutation-containing minimal epitopes. To validate the immunogenicity of these neoepitopes, HLA-transgenic mice were vaccinated using the minimal peptides identified by MS in order to generate neoepitope-reactive TCRs. Specificity of these candidate TCRs was confirmed by peptide titration and recognition of transduced targets.ResultsWe identified precise neoepitopes derived from mutated isoforms of KRAS, EGFR, BRAF, and PIK3CA presented by HLA-A*03:01 and/or HLA-A*11:01 across multiple biological replicates. From our MS data, we were able to successfully isolate murine TCRs that specifically recognize four HLA-A*11:01 restricted neoepitopes (KRAS G13D, PIK3CA E545K, EGFR L858R and BRAF V600E) and three HLA-A*03:01 restricted neoepitopes (KRAS G12V, EGFR L858R and BRAF V600E).ConclusionsOur data show that an MS approach can be used to demonstrate which shared oncogene-derived neoepitopes are processed and presented by common HLA alleles, and those MS data can rapidly be used to develop TCRs against these common tumor-specific antigens. Although further characterization of these neoepitope-specific murine TCRs is required, ultimately, they have the potential to be used clinically for adoptive cell therapy.
Risk factors, outcomes and healthcare utilisation in individuals with multimorbidity including heart failure, chronic kidney disease and type 2 diabetes mellitus: a national electronic health record study
Authors: Pasea Laura , Dashtban Ashkan , Mizani Mehrdad , Bhuva Anish , Morris Tamsin , Mamza Jil Billy , Banerjee Amitava 
Published: September 2023

BackgroundHeart failure (HF), type 2 diabetes (T2D) and chronic kidney disease (CKD) commonly coexist. We studied characteristics, prognosis and healthcare utilisation of individuals with two of these conditions.MethodsWe performed a retrospective, population-based linked electronic health records study from 1998 to 2020 in England to identify individuals diagnosed with two of: HF, T2D or CKD. We described cohort characteristics at time of second diagnosis and estimated risk of developing the third condition and mortality using Kaplan-Meier and Cox regression models. We also estimated rates of healthcare utilisation in primary care and hospital settings in follow-up.FindingsWe identified cohorts of 64 226 with CKD and HF, 82 431 with CKD and T2D, and 13 872 with HF and T2D. Compared with CKD and T2D, those with CKD and HF and HF and T2D had more severe risk factor profile. At 5 years, incidence of the third condition and all-cause mortality occurred in 37% (95% CI: 35.9%, 38.1%%) and 31.3% (30.4%, 32.3%) in HF+T2D, 8.7% (8.4%, 9.0%) and 51.6% (51.1%, 52.1%) in HF+CKD, and 6.8% (6.6%, 7.0%) and 17.9% (17.6%, 18.2%) in CKD+T2D, respectively. In each of the three multimorbid groups, the order of the first two diagnoses was also associated with prognosis. In multivariable analyses, we identified risk factors for developing the third condition and mortality, such as age, sex, medical history and the order of disease diagnosis. Inpatient and outpatient healthcare utilisation rates were highest in CKD and HF, and lowest in CKD and T2D.InterpretationHF, CKD and T2D carry significant mortality and healthcare burden in combination. Compared with other disease pairs, individuals with CKD and HF had the most severe risk factor profile, prognosis and healthcare utilisation. Service planning, policy and prevention must take into account and monitor data across conditions.
Future prospects of biocleaning application in textile conservation
Authors: Jedrusik Aldona , Krasnan Vladimir , Rehakova Milena , Rebros Martin 
Published: September 2023

AbstractStudies of sustainable preservation methods are an important element of ongoing research into minimising the environmental impact of conservation treatment. Of these methods, the cleaning of antique surfaces using selected microbial cultures is attracting attention in the field of heritage conservation. Due to the highly specific nature of the action of these microorganisms, which is similar to enzymatic cleaning, it is generally assumed that individual cultures can remove dirt without endangering the complex structures of textiles. The emphasis is placed on the use of nonpathogenic microbial cultures that have proven to be effective in the cleaning of other historical materials, and which are active in a neutral environment and show relevant metabolic activity. The aims of this work were to study the application of Pseudomonas putida to clean iron gall ink staining and the feasibility of using a selected bacterial strain to clean historic textiles. A relevant procedure for the application of this method to the controlled biocleaning of textiles was also developed. The use of water-based gel systems as a matrix for microorganisms seems to be optimal in terms of providing suitable living conditions for the bacteria and maintaining controlled contact with the surface of the object while simultaneously ensuring efficiency. Tests were carried out on appropriately prepared model samples consisting of cotton and silk. The changes emerging on the surface were evaluated using optical microscopy, and the rate of cleaning was assessed using FTIR and colorimetric methods. In addition, FTIR spectroscopy was employed for microbial control after biocleaning. The research demonstrates the feasibility of cleaning iron gall ink from textiles with viable microbial cells. The selected microorganism was able to reduce undesired discolouration from iron gall ink on model textiles. The results indicate that P. putida has a profound impact on silk samples, and prove that microbial cleaning can achieve a high level of efficiency in the removal of concentrated dirt.
A Cadaveric Feasibility Study of an Automatic Multi-Degrees of Freedom Surgical Robot for Percutaneous Scaphoid Guide Pin Insertion
Authors: WANG Qiao , SHUAI Mei , XIONG Ge , JIA Hongye 
Published: September 2023

Background: Percutaneous fixation of scaphoid fractures need accurate guide pin insertion. The emergence of computer-assisted navigation and robotic surgery may provide a promising solution to this problem. Methods: This study presents the development of an automatic multi-degrees of freedom (DOF) surgical robot with computer-assisted navigation system, focusing on percutaneous scaphoid guide pin insertion. Using this device, along with a 3D fluoroscopy unit, we have conducted an experimental study on 10 cadavers for percutaneous scaphoid guide pin insertion to verify the feasibility and reliability of the system. Results: The mean operative duration was 29.1 (SD 4.3) minutes. The cadavers required no more than two attempts to achieve desired wire placement, with the mean positioning-error being 2.0 (SD 0.3) mm and the mean angle-deviation 3.6 (SD 0.7)°. Throughout the study, a mean of 2.2 full-cycle fluoroscopy attempts was required for each cadaver during surgery, and no preoperative CT scan was needed. Conclusions: The outcomes show that using the automatic surgical robot to perform the percutaneous scaphoid guide pin insertion is feasible and desired results can be achieved.
Ulnar-Sided Upper Extremity Traumatic Wounds: What Should We Expect to Find?
Authors: COSTA João Q. , LEITE Maria J. , RELVAS Miguel , VIEIRA Paula , NEGRÃO Pedro , VIDINHA Vitor 
Published: September 2023

Background: Upper limb traumatic injuries have a significant impact on social and professional life; however, there is still a paucity of studies focusing on the injuries of the ulnar border of the forearm, wrist and hand. Methods: We designed a retrospective single-blinded study, including all patients with deep traumatic wounds affecting the ulnar side of the forearm, wrist or hand, that received surgical treatment from 2006 until 2016. A characterisation of the sample, assessment of concomitant injuries and clinical outcomes, as well as neurological and functional evaluation were performed. Results: We obtained a sample of 61 patients, 69% with injuries affecting the wrist and 90% of patients with a neurological lesion, most frequently of the ulnar nerve lesion (UNL). Concomitant injuries included tendinous lesions, more frequently of the flexor carpi ulnaris (64%) and fractures (13%). And 39% of patients presented an ulnar artery lesion, without significant differences in outcomes regarding the completion of arteriorrhaphy or not. At the end of the 8.6 years follow-up, 34% of patients had no deficits; however, patients with UNL showed worse functional scores and greater risk of sequelae. Besides motor function compromise, sensory deficits were also associated with worst functional outcomes. Conclusions: The UNL subgroup showed important impairment of the first ray, probably related to the level of UNL. Furthermore, besides the implications of the motor sequelae, sensory deficits were also associated with worst functional scores. Due to the high percentage of neurovascular and tendinous lesions in ulnar-sided upper extremity wounds, the authors recommend surgical exploration of these lesions. Level of Evidence: Level IV (Therapeutic)
Case Series: Wide-Awake Local Anaesthesia Without Tourniquet (WALANT) for Camitz Transfer
Authors: NIEMPOOG Sunyarn , JAROENPORN Woraphon 
Published: September 2023

Background: Wide-Awake Local Anaesthesia Without Tourniquet (WALANT), with the benefit of simplicity and a low complication rate, is a well-recognised procedure in orthopaedic surgery. To date, there is no report using WALANT technique with the Camitz transfer procedure. The aim of this study is to determine the early outcomes of using WALANT for Camitz transfer and compare these with outcomes of similar procedures in literature. Methods: Between January 2012 and January 2018, all patients with severe carpal tunnel syndrome (CTS) with thenar muscle atrophy who underwent the Camitz transfer procedure as day surgery under the WALANT technique at the Thammasat University Hospital were included. With the WALANT technique, the 10 mL of prepared anaesthesia was injected into the area around the palmaris longus tendon from 5-cm proximal to the distal wrist crease to the distal palmar crease, while an additional 10 mL was injected from the radial side of the metacarpophalangeal joint of the thumb to the distal wrist crease before the operation. Upon completion of the operation, the patients’ hand was placed in a thumb spica splint. Patient demographic data, visual analogue scale (VAS) during injection and during procedure, operative time, blood lost and complications were recorded. Results: Thirty-six patients (8 males and 28 females) were included. The average age of the patients was 65.9 (37–87) years old. The average VAS score during the anaesthetic injection was 6.22 (5–8). The average VAS pain score during the operation was 0.52 (0–3). The average operative time was 27.06 (17–47) minutes. The mean intraoperative blood loss was 3 (2.4–6.8) mL. All patients underwent the procedure without any complications. Conclusions: The WALANT technique is safe and can be used as alternative anaesthesia for the Camitz transfer. Level of Evidence: Level IV (Therapeutic)
A Comparison of Distal Nerve Blocks and Brachial Plexus Blocks in Terms of Block Success, Block-related Characteristics, Time to Discharge and Patient Satisfaction
Authors: CAKMAK Hamiyet Şenol , ERTAS Gamze , AKDENİZ Sevda , POLAT Ebru , SONMEZ Ayhan , GUMUS Murat 
Published: September 2023

Background: Ultrasound-guided brachial plexus blocks (BPB) are used as an anaesthetic method in hand and wrist surgeries. The radial, median and ulnar nerves can also be selectively blocked. The objective of this retrospective cohort study was to compare distal nerve blocks (DNB) and BPB in terms of block success, block-related characteristics, time to discharge and patient satisfaction. Methods: The data of patients who underwent elective hand and wrist surgery under regional anaesthetic procedures between 01.01.2022 and 01.09.2022 were analysed. Standard multimodal analgesia was performed in all groups, in addition to either ultrasound-guided BPB or DNB. Demographic characteristics, American Society of Anaesthesiology (ASA) classes, presence of additional diseases, block and volume applied, block-related data such as block performance time, onset time, initial analgesia times, perioperative additional anaesthetic or analgesic requirement, surgery times, types of surgery, discharge times and the presence of additional complaints were recorded. In addition, the anaesthesia quality score were determined using a 5-point Likert scale. Results: There was no difference between demographic data. Average surgical time was similar between the DNB and BPB groups (46.0 ± 8.92 vs. 59.95 ± 22.04 min, p < 0.05). Block onset time was significantly lower in the DNB group (26.4 ± 2.73 vs. 32.17 ± 2.94 min, p < 0.001). When patients discharged before and after 12 hours were compared, 11/22 of the patients in the DNB group and 4/23 of the patients in the BPB group were discharged in the first 12 hours (p < 0.05). Patient satisfaction scores were similar between groups (14.22 ± 0.86 vs. 13.65 ± 1.11, p > 0.05). Conclusions: In surgeries of the hand and wrist, the radial, median and ulnar nerves can be used individually or in combination as an alternative to proximal BPB. Level of Evidence: Level III (Therapeutic)
Distal Interphalangeal Joint Arthroplasty: A Narrative Review
Authors: MODI Devam , SLEVIN Omer , BHALLA Hargun , MCCOMBE David B. , BERGER Anthony C. , THAM Stephan K. Y. 
Published: September 2023

Background: Symptomatic distal interphalangeal (DIP) joint arthritis is frequently treated by arthrodesis, though DIP arthroplasty has been reported as a treatment option since 1977. This study reviews the current evidence on DIP joint arthroplasty for the treatment of arthritis refractory to non-operative management. Methods: A systematic search of PubMed, MEDLINE and Embase databases was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Risk of bias was assessed using the ROBINS-I tool. Results: The search yielded 55 records, of which six studies were included in the narrative review. All the included studies were of level IV evidence (case series or cohort studies). DIP arthroplasty was effective in relieving pain and reducing subsequent dysfunction. The average total arc of motion was 30°–40° but with an extension lag of 10°–15°. The overall complication rate was 15% with a re-operation rate of 8%. Joint instability (incidence of 2.5%) and infection (incidence of 2.1%) were the most common complications, while implant fracture was seen in 1% of cases. Joints that failed after DIP arthroplasty were salvaged by DIP arthrodesis. Conclusions: DIP arthroplasty is an effective treatment for painful arthritis but with a complication rate of 15%. Its main advantage over arthrodesis is the preservation of DIP motion. However, due to the limited high-quality evidence available, its use should be limited to circumstances where there is a desire or vocational need to maintain motion at the DIP joint. Level of Evidence: Level V (Therapeutic)
Lunate Pseudarthrosis: Our Clinical Experience
Authors: TEODONNO Francesca , ARNAOUT Ahlam , MERLINI Lorenzo , GRAS Mathilde 
Published: September 2023

Background: Lunate pseudarthrosis is a complication of lunate fractures and few cases are described in the literature. We designed a case series of lunate pseudarthrosis, to acknowledge this topic and to show our clinical experience. Methods: Four patients were included in this study. Three went through surgical treatment of lunate nonunion. Results: At 2 years’ follow-up, patients had slight improvement of range of motion (ROM) and Quick-DASH score compared to preoperative measures, though two patients developed scaphocapitate, radiolunate or midcarpal osteoarthritis. The fourth patient is asymptomatic and is being followed up. Conclusions: Surgical options depend on the localization of the pseudarthrosis in the lunate. Fragment excision can be a good option when the fragment is small, preserving scapholunate (SL) stability. Caution must be taken with dorsal pole pseudarthrosis, regarding midcarpal osteoarthritis. Level of Evidence: Level IV (Therapeutic)
The Incidence of Heterotopic Ossification in Surgically and Non-surgically Treated Elbow Fractures at a Municipal Hospital in Japan
Authors: HIRAKAWA Akihiro , KOMURA Shingo , KURAMITSU Norishige , OHNO Yoshiyuki , AKIYAMA Haruhiko 
Published: September 2023

Background: Heterotopic ossification (HO) is a well-recognised complication of after elbow trauma. The prevalence and risk factors of HO have been previously reported. However, these reports were based on elbow trauma that had undergone surgical treatment and most of them were from Western countries. This study aimed to assess the incidence of HO in patients with elbow fractures who were treated surgically and non-surgically in Japan. Methods: We retrospectively identified consecutive patients who were treated of elbow fractures and fracture–dislocations at our institution in recent consecutive 3-year periods. We extracted patient demographics, injury mechanisms and treatment details from the medical records. Furthermore, we reviewed radiographs to classify the fracture pattern and identify the presence or absence of HO. Results: HO was identified in 6/97 (6%) patients. Fracture–dislocation was noted in 4/6 patients. The fracture types with HO included terrible triad injury (n = 2), isolated coronoid process fractures (n = 2), distal humerus A-type fractures (n = 1) and radial head fracture (n = 1). According to the Hastings and Graham classification, HO was classified as Class I in five patients and Class II B in one patient who underwent additional surgery for HO resection. Conclusions: The incidence of HO was relatively low in our patients. However, of the 20 conservatively treated elbows, one patient developed clinically relevant HO and required excision of HO. Even patients with elbow fractures treated conservatively should be informed of the potential risk of developing severe HO requiring surgical excision. In addition, surgeons in this region could use these data to inform patients about the risk of HO development after an elbow injury. Level of Evidence: Level IV (Therapeutic)
Swan-Neck Correction by FDS Tenodesis and Dynamic Lateral Band Volar Relocation in the Setting of Cerebral Palsy
Authors: ALEXANDER William , THOMAS Damon , ATKINS Sara , JOHNSTONE Bruce 
Published: September 2023

While there are many proposed surgical treatment options for the correction of swan-neck deformities, none are perfect. We describe a partial flexor digitorum superficialis tenodesis that combines both a static volar plate with a dynamic oblique retinacular ligament vector reconstruction. This is performed through a single, short mid-lateral incision and requires no tendon grafts. The protected early active exercises are encouraged postoperatively, and our long-term results have been promising. The technique was designed for children with cerebral palsy, but the indications have since expanded. Level of Evidence: Level V (Therapeutic)
Pectoralis Major Reinnervation by Retrograde Nerve Regeneration after Complete Paralysis of the Brachial Plexus
Authors: BERTELLI Jayme A. , SHAH Harsh R. , LANZARIN Leonardo D. 
Published: September 2023

Spontaneous neuronal recovery is an expected phenomenon in brachial plexus palsy patients. However, the spontaneous recovery owing to retrograde regeneration is an evolving phenomenon with dearth of adequate research on it. Pectoralis major contraction on stimulation of median nerve (in the arm) is an unexpected phenomenon, in the absence of any anomalous communication and with distal coaptation site of intraplexal nerve repair. We presumably attribute it to the retrograde regrowth of axons. The case described supports our hypothesis. Level of Evidence: Level V (Therapeutic)
Changes in the Attributes and Consumer Preferences of Hungarian Hallyu Fans
Authors: Gajzágó Éva Judit 
Published: September 2023

Abstract
The popularity of Korean culture has increased significantly in the last two decades. This process in general is called ‘Hallyu’. The success of it is based on the professional marketing activity of companies, which use exceptional strategies and activities to reach and motivate their customers. This article introduces the diffusion of the culture of South Korea in Hungary, focusing on the specific marketing aspect of consumer behaviour. The author implemented two pieces of scientific research—one in 2018 and another one in 2022—examining this topic. Comparing the results of the two pieces of research gives an opportunity to examine the changes in the diffusion of Hallyu in this country. The fans of Korea and its culture are a relatively small group in Hungarian society but have specific consumer attributes. Hallyu content (such as music, films, and TV series) consumption is fan based, and the behaviour of customers has specific feature, such as the active usage of social media or a strong interest in other Korean products. Hungarian fans have other unique attributes, like enthusiasm and a close relationship with the Korean language, or the emphasis of personal communication. The article introduces these specialities in detail.
The Intersections of Knowledge: Hobbes, Mersenne, Descartes
Authors: Ariew Roger 
Published: September 2023

Abstract
Gregorio Baldin’s book, La croisée des savoirs, concerns the intellectual relations among Hobbes, Mersenne, and Descartes. The study is limited to the time between 1634 and 1648, starting when Hobbes first met Mersenne in Paris and ending when Mersenne died. It covers three main topics. Part i is devoted to the relations maintained by Hobbes with the circle of Mersenne during 1634–1636, which Baldin thinks are essential for the development of Hobbes’ scientific thought. Part ii develops the theme of the “hidden” influence of Mersenne on Hobbes’ thought, highlighting points of contact between the two authors, such as mechanism, research in optics, and their approach to epistemology. Part iii concerns Hobbes’ influence on Mersenne’s thought. It addresses several themes, starting with Mersenne’s interest in Hobbes’ system, the attitudes of Mersenne, Gassendi, and Hobbes toward Cartesian metaphysics, and ending with scientific themes debated in Mersenne’s circle during the 1640s.