bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Mdm2-p53 Axis Links Cementocyte Survival to Cellular Cementum Volume
Authors: Tian Xue , Yang Guobin , Zheng Huiwen , Pi Yixing , Cao Zhengguo , Duan Peipei , Chen Zhi , Yuan Guohua 
Published: February 2025

Abstract
Cementocytes are terminally differentiated cells embedded in cellular cementum, an important hard tissue covering the apical regions of tooth roots. However, the roles of cementocytes in cellular cementum remain enigmatic. Here, we show that Murine Double Minute 2 (Mdm2), an E3 ubiquitin ligase that plays vital roles in regulating cell proliferation, apoptosis, and differentiation to influence tissue or organ development, is highly expressed in the cementocytes of mice. To investigate the role of cementocyte-expressed Mdm2, Dmp1-Cre;Mdm2flox/flox (Mdm2 cKO)mice were obtained to inactivate Mdm2 in cementocytes. The results showed that Mdm2 was successfully ablated and Mdm2 cKO mice display increased cementocyte apoptosis and reduced cellular cementum volume. p53, the canonical substrate of Mdm2, was accumulated and hyperactivated in the cementocytes of Mdm2 cKO mice and in cultured IDG-CM6 cells (a cementocyte cell line) treated with Nutlin3a, an inhibitor of Mdm2. Further experiments showed that inactivation of one allele of p53 significantly rescued the increased cementocyte apoptosis and the decreased cellular cementum volume in Mdm2 cKO mice. Therefore, p53 is targeted by Mdm2 for degradation and mediates the role of Mdm2 in cementocyte survival and cellular cementum volume. Notably, Mdm2 cKO mice exhibited decreased differentiation of cementoblasts (the cell type primarily responsible for cementum deposition) and reduced rate of cellular cementum deposition. Meanwhile, OCCM-30 cells (a cementoblast cell line) showed diminished migration, proliferation, differentiation, and mineralization ability after culture with conditioned medium (CM) from Nutlin3a-pretreated IDG-CM6 cells. Intriguingly, Mdm2 cKO mice displayed significantly increased osteoclast formation and cementum resorption. Meanwhile, in vitro experiments verified that CM from Nutlin3a-pretreated IDG-CM6 cells induced osteoclast differentiation of bone marrow macrophages. Collectively, these results demonstrate that Mdm2-mediated degradation of p53 promotes cementocyte survival, and that cementocytes affect the cell behaviors of cementoblasts and osteoclasts through a paracrine mode to modulate cellular cementum volume.
Clinical significance of 4 L lymphadenectomy in solid dominant clinical stage I non-small cell lung cancer in the left upper lobe
Authors: Watanabe Yukio , Hattori Aritoshi , Fukui Mariko , Matsunaga Takeshi , Takamochi Kazuya , Suzuki Kenji 
Published: February 2025

Abstract

Objectives
The significance of station 4 L lymph node dissection (LND) for early-stage non-small cell lung cancer (NSCLC) is unknown.

Methods
We evaluated 342 patients who underwent complete anatomical resection and mediastinal LND for radiologically solid dominant clinical (c)-Stage I left upper lobe NSCLC between 2008 and 2022. Solid dominant was defined as a consolidation tumor ratio &gt;0.5, on thin-section computed tomography. After matching, postoperative complications and outcomes between the 4 L LND and non-4 L LND groups were compared. Predictors of 4 L metastasis was also identified using logistic regression analysis.

Results
4LND was performed in 215 patients (63%), and 4 L metastasis was detected in 11 patients. Matching yielded 108 pairs. Recurrent nerve paralysis was more frequent in the 4LND group (P = 0.02) with no significant differences in the overall survival (OS) (5y OS: 85.2% vs. 86.3%, P = 0.66) and recurrence-free survival (RFS) (5y RFS: 78.4% vs. 78.5%, P = 0.51) between the 4 L LND and non-4 L LND groups. Logistic regression analysis determined solid component size &gt;20 mm (P = 0.02) and pleural indentation (P = 0.02) on computed tomography findings as clinical risk factors for 4 L metastasis. The predictive criteria for 4 L metastasis were defined as solid component size ≤20 mm without pleural indentation (specificity: 100%, positive predictive value: 100%).

Conclusions
4 L LND had a higher frequency of recurrent nerve palsy, although no survival improvement was observed in solid dominant c-Stage I left upper NSCLC. 4 L LND may be omitted for solid component size ≤20 mm without pleural indentation.
Jump Risk Implicit in Options Market
Authors: Chen Qiang , Han Yu , Huang Ying , Jiang George J 
Published: February 2025

Abstract
We propose a simple procedure to recover (semi-)moments and cumulants from option data. We further derive jump risk measures based on a general asset return model with double-exponential jumps. Numerical and empirical results show that our jump variation measures outperform existing measures under specific conditions. Using return and option data on the S&P 500 index, we examine the information content of our measures, with a focus on large jumps (LJ). Our measures contribute to market realized variance and excess return prediction suggested by in- and out-of-sample tests. Accounting for LJ identified by jump variation improves market return forecast, implying a distinct impact of large and non-LJ.
CD73 promotes the maturation of murine NK cells and their survival in the tumor microenvironment
Authors: Parra-Tello Brian , García-Gómez Moira , Rekus-Polanska Eva , Malgue Felipe , Charlín Sebastián , Hernández-Oliveras Andrés , Reyes-Alvarez Javiera , Rosemblatt Mario , Lundqvist Andreas , Lladser Alvaro , Bono María Rosa , Sauma Daniela 
Published: February 2025

Abstract
Natural Killer (NK) cells are crucial in recognizing and eliminating tumor cells, making them pivotal in antitumor responses. Adenosine, the product of ATP hydrolysis mediated by CD39 and CD73 ectonucleotidases, has been reported to reduce the proliferation and maturation of NK cells. In this study, we investigate the expression of CD73 in NK cells and its impact on maturation, phenotype, survival, and function. Our findings reveal that while splenic NK cells express minimal levels of CD73, its expression is induced upon activation and in the tumor microenvironment upon adoptive transfer to tumor-bearing mice. Notably, within the tumor microenvironment, CD73 expression in NK cells correlates with elevated levels of PD-L1 and CD226. Accordingly, analysis of human melanoma datasets uncovers a subset of immature tumor-infiltrating NK cells expressing CD73. To further understand the role of CD73 on NK cells, we used a CD73 knockout (KO) murine model and observed that CD73-deficient NK cells display a more immature phenotype and heightened proliferative activity than wild-type (WT) NK cells. Additionally, CD73-deficient NK cells exhibit elevated levels of P2X7R and reduced CD39 expression, suggesting an increased susceptibility to ATP-induced death. Following adoptive transfer to tumor-bearing mice, CD73KO NK cells are present at a lower frequency but demonstrate similar control over tumor growth compared to WT NK cells. In conclusion, our study demonstrates the upregulation of CD73 in NK cells infiltrating tumors and underscores its role as a checkpoint regulating the functional maturation of NK cells.
‘Dwelling’ as an educational concept
Authors: Nardo Aline 
Published: February 2025

Abstract
This article explores an educational philosophy centred around the notion of ‘dwelling’. Building on the analysis of Heidegger, Roth, Dewey, Emerson, and Haraway, the article reflects on how ‘dwelling’ foregrounds a certain quality of engagement with the present as the focal point of educational theory and practice. To this end, it considers how the concept of ‘dwelling’ can contribute to the development of present-oriented theoretical perspectives in education. In particular, the article seeks to explore the potential of ‘dwelling’ to offer a non-individualistic notion of ‘being present’ in the context of education, with special emphasis on the question of how ‘being present’ might be understood in community with others who are fundamentally different from us. Additionally, drawing from Roth’s example of a science education oriented towards ‘rooting’, and Murdoch’s thinking around ‘unselfing’ through aesthetic experience, the article contemplates how ‘dwelling’ might be cultivated in and through education.
Culture studies of phytoplankton isolated from Sumiling Dam and their bioremediation capacity in aquaculture wastewater
Authors: Balisacan Lynette G , Franco Prima Fe R , Aguinaldo Henedine A , Batuyong Mae Ann R , Hernando-Pagaling Alice Geraldine 
Published: February 2025

Abstract
Sumiling Dam in Sarrat, Ilocos Norte, is home to diverse phytoplankton species. This study aims to isolate and identify phytoplankton species, optimize the cultivation of one phytoplankton under varying light, pH, and temperature conditions, and assess its bioremediation capacity in aquaculture wastewater. Three stations were set up at the site, each with three sampling points spaced 3–5 m apart. From each point, 300 ml of water was collected using standard procedures and transported to the lab for phytoplankton identification. Samples were incubated in algae culture broth for 14 days, streak-plated for isolation, and cultured under light. Microscope examination was used to characterize phytoplankton species, and the identification was verified by a professional. Ten phytoplankton species were identified, with Haematococcus sp. selected for wastewater bioremediation due to its optimal growth under specific conditions (81 µmol m−2 s−1, pH 9, 25°C ± 2°C). Experiments showed that Haematococcus sp. significantly reduced nitrogen and phosphorus in wastewater over 7–14 days, with Treatment 3 (1000 mg/l) achieving the highest growth, chlorophyll, and nitrogen reduction, while Treatment 2 (750 mg/l) excelled in phosphorus removal. These results underscore phytoplankton’s potential for sustainable, large-scale wastewater bioremediation.
Global Least Squares Algorithm for 3D Elemental Surface Topography Reconstruction Using Quad-Segment Detector in X-ray Spectrometry
Authors: Gholami Hatam Ebrahim , Pelicon Primož 
Published: February 2025

Abstract
2D elemental imaging techniques such as micro-X-ray fluorescence (micro-XRF) and micro-particle-induced X-ray emission (micro-PIXE) play a critical role in elemental mapping across diverse fields such as biology, geology, materials science, and engineering. However, surface irregularities often introduce shadow effects, hindering accurate spectrometric analysis. Knowing the topography information is essential for addressing this issue. Here, we propose integrating a global least squares algorithm for reconstructing the 3D surface topography in micro-PIXE analysis which is applicable in other similar techniques based on X-ray microscopy. This algorithm utilizes two independent gradient components, distorted by noise, to calculate the gradient vector from X-ray data acquired by an annular quad-segment spectrometer. We demonstrate the capability of this approach on a real homogeneous sample, yielding 3D elemental surface topography. This noniterative code provides surface reconstructions which in turn could find application to enhance the correction of spatial elemental distributions across heterogeneous sample types.
Drug-resistance genes and antifungal susceptibility of Trichophyton verrucosum variants isolated from bovine skin lesions and farm environments
Authors: Lee Han Gyu , Jung Young-Hun , Cho Ara , Do Yoon Jung , Bok Eun-Young , Oem Jae Ku , Hur Tai-Young 
Published: February 2025

Abstract
Trichophyton verrucosum causes a highly contagious disease in bovines and is occasionally transmitted to humans. Azoles are widely used as antifungal drugs in bovines, and act by targeting the ergosterol biosynthesis pathway. However, cases of treatment failure and recurrence of dermatophyte infections have been associated with mutations at the drug target site. Currently, information on T. verrucosum variants is lacking. This study aimed to classify T. verrucosum variants isolated from animals exhibiting clinical symptoms and cattle environments in the Republic of Korea based on their antifungal susceptibility and the presence or absence of ergosterol biosynthesis (ERG) and subtilisin genes. Of 139 clinical and 39 environmental samples, 86 and 21 were found to be infected, respectively. The positivity rate of calves aged 1–6 months was 73.1%, which was significantly higher than that of calves aged &gt;6 months (55.2%). Twenty-seven T. verrucosum strains were identified as T. album (n = 9), T. ochraceum (n = 6), and T. discoides (n = 12). Antifungal susceptibility testing showed that enilconazole had the lowest geometric mean antifungal activity of 1.08, 1, and 0.94 µg/ml against T. album, T. ochraceum, and T. discoides, respectively. The detection ratios of ERG3 and ERG6 differed significantly among the three variants. In conclusion, our study suggests conducting sample cultures and antifungal susceptibility tests on isolates before administering antifungal drugs. Moreover, the expression profile of ERG appears to have a potential link to drug susceptibility. This information may contribute to the prevention and management of dermatophytosis in cattle.
Photometric and spectroscopic studies of six W UMa-type eclipsing binaries
Authors: Zhang Bin , Gao Yi-Dan , Li Kai , Wang Jing-Jing , Zhang Xu-Dong , Xiao Wen-Jun 
Published: February 2025

ABSTRACT
We performed new photometric and spectroscopic studies on six W UMa-type eclipsing binaries (V342 UMa, LX Leo, V1062 Her, V801 And, 1SWASP J165649.13+402738.4, and V1067 Her). From orbital period analysis, V342 UMa and LX Leo show continuous period decrease with a rate of −2.11($\pm$0.06) $\times$ 10$^{-7}$ and −6.02$(\pm 0.13)$ × 10$^{-8}$ d yr$^{-1}$, respectively. Another four targets show cyclic variation, which could be caused by the light-travel-time effect via the third body. In addition, using the spectral subtraction technique, we analysed the low-resolution Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST) spectra of the five objects and detected the ${\rm{H}} \, {\alpha }$ emission line, which implies chromospheric activity. In addition, the light curves of these systems show an obvious O’Connell effect. Using the Wilson–Devinney code, we also obtained their photometric solutions. It is suggested that V1067 Her is a medium contact binary; others are shallow contact eclipsing binary systems. Based on photometric solutions, Gaia and LAMOST data, we calculated the absolute parameters of these targets. We also discussed their evolutionary status and found that these targets are stable for the moment. The third body may play an important role in the formation and evolution of these short-period eclipsing binaries.
Mining the time axis with TRON – II. MeerKAT detects a stellar radio flare from a distant RS CVn candidate
Authors: Smirnov O M , Golden A , Myburgh T , Ngcebetsha B , Tasse C , Heywood I , Ramaila A J T , Thompson M A , Kenyon J S , Perkins S J , Dawson J , Bester H L , Bright J S , Oozeer N , Samboco V G G , Sihlangu I , Choza C 
Published: February 2025

ABSTRACT
Medium-time-scale (minutes to hours) radio transients are a relatively unexplored population. The wide field-of-view and high instantaneous sensitivity of instruments such as MeerKAT provides an opportunity to probe this class of sources, using image-plane detection techniques. The previous letter in this series describes our project and associated Transient Radio Observations for Newbies (TRON) pipeline designed to mine archival MeerKAT data for transient and variable sources. In this letter, we report on a new transient, a radio flare, associated with Gaia DR3 6865945581361480448, a G type star, whose parallax places it at a distance of 1330 pc. Its duration and high degree of circular polarization suggests electron cyclotron maser instability as the mechanism, consistent with an RS CVn variable.
Combinatorial In Vivo Genome Editing Identifies Widespread Epistasis and an Accessible Fitness Landscape During Lung Tumorigenesis
Authors: Hebert Jess D , Tang Yuning J , Szamecz Márton , Andrejka Laura , Lopez Steven S , Petrov Dmitri A , Boross Gábor , Winslow Monte M 
Published: February 2025

Abstract
Lung adenocarcinoma, the most common subtype of lung cancer, is genomically complex, with tumors containing tens to hundreds of non-synonymous mutations. However, little is understood about how genes interact with each other to enable the evolution of cancer in vivo, largely due to a lack of methods for investigating genetic interactions in a high-throughput and quantitative manner. Here, we employed a novel platform to generate tumors with inactivation of pairs of ten diverse tumor suppressor genes within an autochthonous mouse model of oncogenic KRAS-driven lung cancer. By quantifying the fitness of tumors with every single and double mutant genotype, we show that most tumor suppressor genetic interactions exhibited negative epistasis, with diminishing returns on tumor fitness. In contrast, Apc inactivation showed positive epistasis with the inactivation of several other genes, including synergistic effects on tumor fitness in combination with Lkb1 or Nf1 inactivation. Sign epistasis was extremely rare, suggesting a surprisingly accessible fitness landscape during lung tumorigenesis. These findings expand our understanding of the interactions that drive tumorigenesis in vivo.
Different Classifier Types in One Language
Authors: Aikhenvald Alexandra Y 
Published: February 2025

Abstract
Several distinct classifier types may coexist in one language. The cooccurrence of different classifier systems in different morphosyntactic environments constitutes a strong argument in favour of the proposed typology of classifiers based on the morphosyntactic locus of coding of noun categorization devices (together with their scope of categorization, principles of assignment, and the kind of surface realization: see §1.5).
Noun Categorization Devices in Language Acquisition and Dissolution
Authors: Aikhenvald Alexandra Y 
Published: February 2025

Abstract
The behaviour of noun categorization devices in child language acquisition and language development, as well as in language dissolution, provides important clues as to how such devices are assigned, what the categorical interrelations between them are, and which of them is the most stable. It also provides us with information on the psycholinguistic reality of the assignment of noun categorization devices. In this chapter, we consider language acquisition, development, and dissolution of two kinds of noun categorization device, noun classes and numeral classifiers. We have to restrict ourselves to the discussion of just these two because of the limitations of actual psycholinguistic research undertaken thus far. No experiments have been undertaken on language acquisition or dissolution of other classifier types, or of how these processes take place in multiple classifier systems. These are topics for future study.
RF-EMF electromagnetic environment IN the West Bank, Palestine
Authors: Lahham Adnan , Alkhatib Mahmoud 
Published: February 2025

Abstract
With the increase in the distribution of sources of electromagnetic fields in the environment, public exposure to non-ionizing radiation emitted from these sources will increase and change with time. This work aims at the evaluation of public exposure to radiofrequency electromagnetic fields in the West Bank and compares this exposure with previous studies with a time interval difference of 11 years. Measurements of exposure were conducted in the outdoor environment in 149 locations using tri-axial E-field frequency-selective personal exposure meter EME SPY 140 enabling measurements of electric field strength in 14 predefined frequency bands in the range from 80 to 6 GHz. The average field strength from all sources in all investigated locations was 1.4 Vm−1. The maximum exposure measured at any location in the country was 7.43 Vm−1 and was found in Ramallah City center. The exposure quotient corresponding to this value was about 19 times below unity. The total exposure quotient for all locations was 0.001 with FM broadcasting being the main contributor by about 36%, UMTS2100 downlink by 24%, GSM 900 downlink contributing by 17%, WiFi 5GHz by 9% GSM 1800 downlink by 5%. Seven other RF sources contributed together by only 9% including WiMax, TV, WiFi 2 GHz, and others. More than 90% of the electric field strength values were below the level of 3 Vm−1. Within 11 years the average total exposure coefficient increased by a factor of about 2.
Aberrant Functional Connectivity and Brain Network Organization in High-Schizotypy Individuals: An Electroencephalography Study
Authors: Trajkovic Jelena , Ricci Giulia , Pirazzini Gabriele , Tarasi Luca , Di Gregorio Francesco , Magosso Elisa , Ursino Mauro , Romei Vincenzo 
Published: February 2025

Abstract

Background and Hypothesis
Oscillatory synchrony plays a crucial role in establishing functional connectivity across distinct brain regions. Within the realm of schizophrenia, suggested to be a neuropsychiatric disconnection syndrome, discernible aberrations arise in the organization of brain networks. We aim to investigate whether the resting-state functional network is already altered in healthy individuals with high schizotypy traits, highlighting the pivotal influence of brain rhythms in driving brain network alterations.

Study Design
Two-minute resting-state electroencephalography recordings were conducted on healthy participants with low and high schizotypy scores. Subsequently, spectral Granger causality was used to compute functional connectivity in theta, alpha, beta, and gamma frequency bands, and graph theory metrics were employed to assess global and local brain network features.

Study Results
Results highlighted that high-schizotypy individuals exhibit a lower local efficiency in theta and alpha frequencies and a decreased global efficiency across theta, alpha, and beta frequencies. Moreover, high schizotypy is characterized by a lower nodes’ centrality and a frequency-specific decrease of functional connectivity, with a reduced top-down connectivity mostly in slower frequencies and a diminished bottom-up connectivity in faster rhythms.

Conclusions
These results show that healthy individuals with a higher risk of developing psychosis exhibit a less efficient functional brain organization, coupled with a systematic decrease in functional connectivity impacting both bottom-up and top-down processing. These frequency-specific network alterations provide robust support for the dimensional model of schizophrenia, highlighting distinctive neurophysiological signatures in high-schizotypy individuals.