bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paleogene India-Eurasia collision constrained by observed plate rotation
Authors: Wu Xiaoyue , Hu Jiashun , Chen Ling , Liu Liang , Liu Lijun 
Published: November 2023

AbstractThe Cenozoic India-Eurasia collision has had profound impacts on shaping the Tibetan plateau, but its early history remains controversial due to uneven availability of constraints. Recent plate reconstructions reveal two prominent counterclockwise rotation (azimuthal change) rate peaks of the Indian plate at 52-44 and 33-20 Ma, respectively, which could bear key information about this collision history. Using fully dynamic three-dimensional numerical modeling, we show that the first rotation rate peak reflected the initial diachronous collision from the western-central to eastern Indian front, and the second peak reflected the full collision leading to strong coupling between India and Eurasia. Further comparison with observation suggests that the initial and complete India-Eurasia collision likely occurred at 55 ± 5 and 40 ± 5 Ma, respectively, an inference consistent with key geological observations. We suggest that this collision history is instructive for studying the tectonic history of the Tibetan plateau and its surrounding areas.
Malaria surveillance reveals parasite relatedness, signatures of selection, and correlates of transmission across Senegal
Authors: Schaffner Stephen F. , Badiane Aida , Khorgade Akanksha , Ndiop Medoune , Gomis Jules , Wong Wesley , Ndiaye Yaye Die , Diedhiou Younouss , Thwing Julie , Seck Mame Cheikh , Early Angela , Sy Mouhamad , Deme Awa , Diallo Mamadou Alpha , Sy Ngayo , Sene Aita , Ndiaye Tolla , Sow Djiby , Dieye Baba , Ndiaye Ibrahima Mbaye , Gaye Amy , Ndiaye Aliou , Battle Katherine E. , Proctor Joshua L. , Bever Caitlin , Fall Fatou Ba , Diallo Ibrahima , Gaye Seynabou , Sene Doudou , Hartl Daniel L. , Wirth Dyann F. , MacInnis Bronwyn , Ndiaye Daouda , Volkman Sarah K. 
Published: November 2023

AbstractWe here analyze data from the first year of an ongoing nationwide program of genetic surveillance of Plasmodium falciparum parasites in Senegal. The analysis is based on 1097 samples collected at health facilities during passive malaria case detection in 2019; it provides a baseline for analyzing parasite genetic metrics as they vary over time and geographic space. The study’s goal was to identify genetic metrics that were informative about transmission intensity and other aspects of transmission dynamics, focusing on measures of genetic relatedness between parasites. We found the best genetic proxy for local malaria incidence to be the proportion of polygenomic infections (those with multiple genetically distinct parasites), although this relationship broke down at low incidence. The proportion of related parasites was less correlated with incidence while local genetic diversity was uninformative. The type of relatedness could discriminate local transmission patterns: two nearby areas had similarly high fractions of relatives, but one was dominated by clones and the other by outcrossed relatives. Throughout Senegal, 58% of related parasites belonged to a single network of relatives, within which parasites were enriched for shared haplotypes at known and suspected drug resistance loci and at one novel locus, reflective of ongoing selection pressure.
Operando Li metal plating diagnostics via MHz band electromagnetics
Authors: Ishigaki Masanori , Ishikawa Keisuke , Usuki Tsukasa , Kondo Hiroki , Komagata Shogo , Sasaki Tsuyoshi 
Published: November 2023

AbstractA nondestructive detection method for internal Li-metal plating in lithium-ion batteries is essential to improve their lifetime. Here, we demonstrate a direct Li-metal detection technology that focuses on electromagnetic behaviour. Through an interdisciplinary approach combining the ionic behaviour of electrochemical reactions at the negative electrode and the electromagnetic behaviour of electrons based on Maxwell’s equations, we find that internal Li-metal plating can be detected by the decrease in real part of the impedance at high-frequency. This finding enables simpler diagnostics when compared to data-driven analysis because we can correlate a direct response from the electronic behaviour to the metallic material property rather changes in the ionic behaviour. We test this response using commercial Li-ion batteries subject to extremely fast charging conditions to induce Li-metal plating. From this, we develop a battery sensor that detects and monitors the cycle-by-cycle growth of Li-metal plating. This work not only contributes to advancing future Li-ion battery development but may also serve as a tool for Li-metal plating monitoring in real-field applications to increase the useable lifetime of Li-ion batteries and to prevent detrimental Li-metal plating.
Microstimulation of human somatosensory cortex evokes task-dependent, spatially patterned responses in motor cortex
Authors: Shelchkova Natalya D. , Downey John E. , Greenspon Charles M. , Okorokova Elizaveta V. , Sobinov Anton R. , Verbaarschot Ceci , He Qinpu , Sponheim Caleb , Tortolani Ariana F. , Moore Dalton D. , Kaufman Matthew T. , Lee Ray C. , Satzer David , Gonzalez-Martinez Jorge , Warnke Peter C. , Miller Lee E. , Boninger Michael L. , Gaunt Robert A. , Collinger Jennifer L. , Hatsopoulos Nicholas G. , Bensmaia Sliman J. 
Published: November 2023

AbstractThe primary motor (M1) and somatosensory (S1) cortices play critical roles in motor control but the signaling between these structures is poorly understood. To fill this gap, we recorded – in three participants in an ongoing human clinical trial (NCT01894802) for people with paralyzed hands – the responses evoked in the hand and arm representations of M1 during intracortical microstimulation (ICMS) in the hand representation of S1. We found that ICMS of S1 activated some M1 neurons at short, fixed latencies consistent with monosynaptic activation. Additionally, most of the ICMS-evoked responses in M1 were more variable in time, suggesting indirect effects of stimulation. The spatial pattern of M1 activation varied systematically: S1 electrodes that elicited percepts in a finger preferentially activated M1 neurons excited during that finger’s movement. Moreover, the indirect effects of S1 ICMS on M1 were context dependent, such that the magnitude and even sign relative to baseline varied across tasks. We tested the implications of these effects for brain-control of a virtual hand, in which ICMS conveyed tactile feedback. While ICMS-evoked activation of M1 disrupted decoder performance, this disruption was minimized using biomimetic stimulation, which emphasizes contact transients at the onset and offset of grasp, and reduces sustained stimulation.
Engineering sulfonate group donor regeneration systems to boost biosynthesis of sulfated compounds
Authors: Xu Ruirui , Zhang Weijao , Xi Xintong , Chen Jiamin , Wang Yang , Du Guocheng , Li Jianghua , Chen Jian , Kang Zhen 
Published: November 2023

AbstractSulfonation as one of the most important modification reactions in nature is essential for many biological macromolecules to function. Development of green sulfonate group donor regeneration systems to efficiently sulfonate compounds of interest is always attractive. Here, we design and engineer two different sulfonate group donor regeneration systems to boost the biosynthesis of sulfated compounds. First, we assemble three modules to construct a 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration system and demonstrate its applicability for living cells. After discovering adenosine 5’-phosphosulfate (APS) as another active sulfonate group donor, we engineer a more simplified APS regeneration system that couples specific sulfotransferase. Next, we develop a rapid indicating system for characterizing the activity of APS-mediated sulfotransferase to rapidly screen sulfotransferase variants with increased activity towards APS. Eventually, the active sulfonate group equivalent values of the APS regeneration systems towards trehalose and p-coumaric acid reach 3.26 and 4.03, respectively. The present PAPS and APS regeneration systems are environmentally friendly and applicable for scaling up the biomanufacturing of sulfated products.
Non-just-right experiences are more closely related to OCD than tics in Tourette patients
Authors: Brandt Valerie , Otte Jan-Hendrik , Fremer Carolin , Jakubovski Ewgeni , Müller-Vahl Kirsten 
Published: November 2023

AbstractComplex tics and obsessive or compulsive behaviour can be difficult to differentiate diagnostically. The majority of adult patients with Tourette syndrome report experiencing premonitory urges before tics. Some of these experiences have been linked to non-just-right experiences (NJRE), which are frequently reported by patients with obsessive–compulsive disorder or behaviours (OCD/OCB). We aimed to assess whether NJRE are more closely related to tics and tic-associated premonitory urges or whether they are more closely associated with OCD. A total of N = 111 patients (mean age = 34.77 + /−12.93; N = 37 female) with a confirmed diagnosis of Tourette syndrome completed the premonitory urges for tic disorders scale (PUTS), the revised non-just-right experiences scale (NJRE-QR), and questionnaires regarding their tic severity, and comorbid OCD/OCB. A multi-trait-multi-methods matrix was calculated to examine associations amongst scales measuring tic-related and OCB-related phenomena. The PUTS correlated overall higher with tic questionnaires than with OCD/OCB questionnaires. The NJRE correlated higher with OCD symptoms than with tic severity. The results indicate that non-just-right experiences are more closely associated with comorbid OCB than with tics in patients with Tourette syndrome.
Mannich-type modifications of (−)-cannabidiol and (−)-cannabigerol leading to new, bioactive derivatives
Authors: Lőrincz Eszter Boglárka , Tóth Gergely , Spolárics Júlia , Herczeg Mihály , Hodek Jan , Zupkó István , Minorics Renáta , Ádám Dorottya , Oláh Attila , Zouboulis Christos C. , Weber Jan , Nagy Lajos , Ostorházi Eszter , Bácskay Ildikó , Borbás Anikó , Herczegh Pál , Bereczki Ilona 
Published: November 2023

Abstract(−)-Cannabidiol (CBD) and (−)-cannabigerol (CBG) are two major non-psychotropic phytocannabinoids that have many beneficial biological properties. However, due to their low water solubility and prominent first-pass metabolism, their oral bioavailability is moderate, which is unfavorable for medicinal use. Therefore, there is a great need for appropriate chemical modifications to improve their physicochemical and biological properties. In this study, Mannich-type reaction was used for the synthetic modification of CBD and CBG for the first time, and thus fifteen new cannabinoid derivatives containing one or two tertiary amino groups were prepared. Thereafter the antiviral, antiproliferative and antibacterial properties of the derivatives and their effects on certain skin cells were investigated. Some modified CBD derivatives showed remarkable antiviral activity against SARS-CoV-2 without cytotoxic effect, while synthetic modifications on CBG resulted in a significant increase in antiproliferative activity in some cases compared to the parent compound.
Cannabis use and the risk of primary open-angle glaucoma: a Mendelian randomization study
Authors: Katsimpris Andreas , Baumeister Sebastian-Edgar , Baurecht Hansjörg , Tatham Andrew J. , Nolde Michael 
Published: November 2023

AbstractSeveral observational studies have investigated the association between cannabis use and intraocular pressure, but its association with primary open-angle glaucoma (POAG) remains unclear. In this study, we leveraged human genetic data to assess through Mendelian randomization (MR) whether cannabis use affects POAG. We used five single-nucleotide polymorphisms (SNPs) associated with lifetime cannabis use (P-value < 5 × 10–8) from a genome-wide association study (GWAS) (N = 184,765) by the International Cannabis Consortium, 23andMe, and UK Biobank and eleven SNPs associated with cannabis use disorder (P-value < 5 × 10–7) from a GWAS meta-analysis of (17,068 cases and 357,219 controls of European descent) from Psychiatric Genomics Consortium Substance Use Disorders working group, Lundbeck Foundation Initiative for Integrative Psychiatric Research, and deCode. We associated the selected five SNPs from the GWAS of lifetime cannabis use and the eleven SNPs from the GWAS of cannabis use disorder, with the largest to date GWAS meta-analysis of POAG (16,677 cases and 199,580 controls). MR analysis suggested no evidence for a causal association of lifetime cannabis use and cannabis use disorder with POAG (odds ratio (OR) of outcome per doubling of the odds of exposure (95% confidence interval): 1.04 (0.88; 1.23) for lifetime cannabis use and 0.97 (0.92; 1.03) for cannabis use disorder). Sensitivity analyses to address pleiotropy and weak instrument bias yielded similar estimates to the primary analysis. In conclusion, our results do not support a causal association between cannabis use and POAG.
An analysis of slippage effects on a solid sphere enclosed by a non-concentric cavity filled with a couple stress fluids
Authors: Al-Hanaya Amal , El-Sapa Shreen 
Published: November 2023

AbstractThis investigation shows the effect of slippage on the slow spinning of a rigid sphere covered by a non-concentric spherical hollow full of an incompressible couple stress fluid. Moreover, the velocity slip conditions are employed on surfaces of both the rigid sphere and the cavity. In addition, the solid sphere and the cavity are rotating axially at various angular speeds. The solution is obtained semi-analytically at low Reynolds numbers utilizing the superposition with the numerical collocation approach. This paper discusses the hydrodynamic couple exerted by the fluid on the internal particle. The dimensionless torque increases as the slip and spin slip increase by 99%, the couple stress parameter by 49%, and the separation parameter by 79%. Additionally, the non-dimensional torque decreases with the increase of the size ratio by 89%. Consequently, it is found that all the results agreed with the corresponding numerical analysis in the traditional viscous liquids and the revolving of two eccentric rigid spheres with no slippage (Al-Hanaya et al. in J. Appl Mech Tech Phys 63(5):1–9, 2022).
Clinacanthus nutans genetic diversity and its association with anti-apoptotic, antioxidant, and anti-bacterial activities
Authors: Chiangchin Salinee , Thongyim Saruda , Pandith Hataichanok , Kaewkod Thida , Tragoolpua Yingmanee , Inta Angkhana , Watthana Santi , Pongamornkul Wittaya , Jangsutthivorawat Siriphorn , Panya Aussara 
Published: November 2023

AbstractClinacanthus nutans (Burm. f.) Lindau has been extensively utilized in Thai folk medicine. However, there has been no prior exploration of its genetic diversity or its correlation with biological activity and phytochemical profiles. Herein, a total of 10 samples of C. nutans were collected from different geographic locations in different environments of Thailand, encompassing Northern, Northeastern, and Central regions. The genetic diversity study using sequence-related amplified polymorphism (SRAP) markers showed that all C. nutans samples were closely related, as indicated by UPGMA cluster analysis. When comparing the biological activities of C. nutans extracts, our findings demonstrated that those sourced from Northern Thailand exhibited the most potent activity in reducing lipopolysaccharide-inducing cell death, as accessed by cell viability assay. Furthermore, they showed remarkable antioxidant and antibacterial activities against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. High-performance liquid chromatography (HPLC) analysis of phytochemical profiles revealed consistent chromatography peak patterns across all C. nutans extracts. However, they exhibited varying levels of phenolic contents, as judged by the Folin-Ciocalteu assay, which positively correlated with their observed activities. In conclusion, this study highlights the limited genetic variation within C. nutans population in Thailand. Furthermore, it underscores the association between the biological activity and the total phenolic contents which might be mainly impacted by environmental conditions.
IL-4 and IL-13 induce equivalent expression of traditional M2 markers and modulation of reactive oxygen species in human macrophages
Authors: Scott Tara E. , Lewis Caitlin V. , Zhu Mingyu , Wang Chao , Samuel Chrishan S. , Drummond Grant R. , Kemp-Harper Barbara K. 
Published: November 2023

AbstractIn cardiovascular disease, pathological and protective roles are reported for the Th2 cytokines IL-4 and IL-13, respectively. We hypothesised that differential effects on macrophage function are responsible. Type I and II receptor subunit (IL-2Rγ, IL-4Rα and IL-13Rα1) and M2 marker (MRC-1, CCL18, CCL22) expression was assessed via RT-qPCR in IL-4- and IL-13-treated human primary macrophages. Downstream signalling was evaluated via STAT1, STAT3 and STAT6 inhibitors, and IL-4- and IL-13-induced reactive oxygen species (ROS) generation assessed. IL-4 and IL-13 exhibited equivalent potency and efficacy for M2 marker induction, which was attenuated by STAT3 inhibition. Both cytokines enhanced PDBu-stimulated superoxide generation however this effect was 17% greater with IL-4 treatment. Type I IL-4 receptor expression was increased on M1-like macrophages but did not lead to a differing ability of these cytokines to modulate M1-like macrophage superoxide production. Overall, this study did not identify major differences in the ability of IL-4 and IL-13 to modulate macrophage function, suggesting that the opposing roles of these cytokines in cardiovascular disease are likely to be via actions on other cell types. Future studies should directly compare IL-4 and IL-13 in vivo to more thoroughly investigate the therapeutic validity of selective targeting of these cytokines.
Subtyping of COVID-19 samples based on cell-cell interaction in single cell transcriptomes
Authors: Jeong Kyeonghun , Kim Yooeun , Jeon Jaemin , Kim Kwangsoo 
Published: November 2023

AbstractIn single-cell transcriptome analysis, numerous biomarkers related to COVID-19 severity, including cell subtypes, genes, and pathways, have been identified. Nevertheless, most studies have focused on severity groups based on clinical features, neglecting immunological heterogeneity within the same severity level. In this study, we employed sample-level clustering using cell-cell interaction scores to investigate patient heterogeneity and uncover novel subtypes. The clustering results were validated using external datasets, demonstrating superior reproducibility and purity compared to gene expression- or gene set enrichment-based clustering. Furthermore, the cell-cell interaction score-based clusters exhibited a strong correlation with the WHO ordinal severity score based on clinical characteristics. By characterizing the identified subtypes through known COVID-19 severity-associated biomarkers, we discovered a “Severe-like moderate” subtype. This subtype displayed clinical features akin to moderate cases; however, molecular features, such as gene expression and cell-cell interactions, resembled those of severe cases. Notably, all patients who progressed from moderate to severe belonged to this subtype, underscoring the significance of cell-cell interactions in COVID-19 patient heterogeneity and severity.
Experimental production of K-rich metasomes through sediment recycling at the slab-mantle interface in the fore-arc
Authors: Gülmez Fatma , Prelević Dejan , Förster Michael W. , Buhre Stephan , Günther Jennifer 
Published: November 2023

AbstractSediment contribution to the mantle is the key step for the generation of orogenic magmatism to produce its isotopic and geochemical inventory. Even though they are exceptional for the post-collisional settings, there are worldwide examples of arc-related ultrapotassic mafic magmas which require complex multi-stage processes along with sediment melting e.g. in Italy or Pontides of Türkiye. To understand the metasomatism leading mantle to produce ultrapotassic mafic melts, we simulated the reactions of depleted (harzburgite) and fertile (lherzolite) mantle with subducted carbonate-rich sediment at relatively cold (800–850 °C) and shallow (2 GPa, 60–80 km) slab-mantle interfaces. The melting of sediments can trigger the formation of immiscible and conjugate carbonatitic and silicic melts which flux the mantle to develop hydrous minerals and dolomitic melt. The metasomatic growth product is a wehrlite composed of clinopyroxene, phlogopite, carbonate minerals and amphibole, representing a source of choice for Si-undersaturated ultrapotassic lavas. The occurrence of conjugate carbonatitic and silicic melts and their potential physical separation, offer a possibility for fractionation of several canonical trace element ratios such as Th/La, observed in Si-saturated ultrapotassic lavas. The synergy between peridotite-melt interaction and the physical separation of the carbonatitic and extremely K-enriched silicic melts are essential for the compositional evolution of ultrapotassic orogenic magmas and their mantle sources.
Activation-induced cytidine deaminase an antibody diversification enzyme interacts with chromatin modifier UBN1 in B-cells
Authors: Jaiswal Ankit , Roy Rajarshi , Tamrakar Anubhav , Singh Amit Kumar , Kar Parimal , Kodgire Prashant 
Published: November 2023

AbstractActivation-induced cytidine deaminase (AID) is the key mediator of antibody diversification in activated B-cells by the process of somatic hypermutation (SHM) and class switch recombination (CSR). Targeting AID to the Ig genes requires transcription (initiation and elongation), enhancers, and its interaction with numerous factors. Furthermore, the HIRA chaperon complex, a regulator of chromatin architecture, is indispensable for SHM. The HIRA chaperon complex consists of UBN1, ASF1a, HIRA, and CABIN1 that deposit H3.3 onto the DNA, the SHM hallmark. We explored whether UBN1 interacts with AID using computational and in-vitro experiments. Interestingly, our in-silico studies, such as molecular docking and molecular dynamics simulation results, predict that AID interacts with UBN1. Subsequently, co-immunoprecipitation and pull-down experiments established interactions between UBN1 and AID inside B-cells. Additionally, a double immunofluorescence assay confirmed that AID and UBN1 were co-localized in the human and chicken B-cell lines. Moreover, proximity ligation assay studies validated that AID interacts with UBN1. Ours is the first report on the interaction of genome mutator enzyme AID with UBN1. Nevertheless, the fate of interaction between UBN1 and AID is yet to be explored in the context of SHM or CSR.