bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative pulmonary rehabilitation in medical inoperable patients with early stage non-small cell lung cancer and postoperative results
Authors: HAZER Seray , SATAR Seher , CANDEMİR İpek , ERGÜN Pınar , ACAR Leyla Nesrin , GULHAN Selim Şakir Erkmen 
Published: October 2023

Aims: The impact of postoperative complications after surgery for lung cancer is substantial, with the increasing age of patients and the presence of comorbidities. Impairment in exercise capacity is a potential modifiable risk factor for postoperative complications. This study aimed to assess the contribution of preoperative pulmonary rehabilitation (PR) for increasing operability conditions in non-small cell lung cancer (NSCLC) for patients with limited pulmonary functions and postoperative results.
Methods: The patients with NSCLC who had preoperative pulmonary rehabilitation and underwent surgical resection in our clinics between 2010-2019 were evaluated retrospectively. The patients enrolled in a comprehensive, multidisciplinary, supervised outpatient 10-day duration PR program preoperatively, consisting of bronchial hygiene, breathing control, energy conservation techniques, exercise training (endurance and strength), psychological support, and nutritional support. Exercise capacity and VO2 peak were evaluated by using an incremental shuttle walk test (ISWT).
Results: Eighteen patients who underwent surgery due to NSCLC and had a pre-operative pulmonary rehabilitation program were evaluated. All the cases were male; the mean age was 66.2 (53-77) years. The squamous cell/adenocarcinoma ratio was 2.6 (13:5), and the mean tumor size was 4.6 (8-18) cm. The postoperative hospital stay was 12.7 (4-42) days, and they were followed up for an average of 30.2 (2-83) months.
Conclusion: Complete surgical resection is the most effective curative treatment for lung cancer. However, many patients with lung cancer also have severe COPD, increasing their risk of postoperative complications and their likelihood of being considered "inoperable." Preoperative pulmonary rehabilitation (PR) has been proposed as an intervention for risk modification and to decrease surgical morbidity and mortality. The results of our study also revealed the importance of preoperative pulmonary rehabilitation in centers where lung cancer surgery was performed.
Reversal Error in Mathematics: Training Against It Using ε⃔  coach
Authors: Santamarta Vicent , Garcia-Segarra Pablo , Ventura-Campos Noelia , Moreno-Rus Aida , Falomir Zoe 
Published: October 2023

This paper presents ε⃔  coach, a serious game prototype which aims at training players to detect and avoid reversal error when representing word problems of the type “N times as many S as P” algebraically as an equation. The ε⃔  coach visualizes the relative size of the entities in the relation following a qualitative model based on containers. This serious game also provides a quantitative visualization of the entities related using graphics, it helps players to resolve the formulated equation using discrete values and it shows players’ progress.
Age and breed susceptibility of dogs to the causative agent of cystoisosporosis
Authors: Suvorov R. , Melnychuk V. 
Published: October 2023

Cystoisosporosis refers to a protozoan parasitic disease caused by coccidia of the genus Cystoisospora canis in dogs, especially young ones. Pathogens are localized in the small intestine of animals and lead to an imbalance of microflora, indigestion, enteritis, and diarrhea. Knowledge of the epizootological features of this invasion makes it possible to carry out complex preventive measures more effectively and maintain well-being in certain territories. The research aimed to establish the dependence of the infection of dogs with the causative agent of cystoisosporosis on their age and breed. The conducted studies established that the age-related dynamics of cystoisosoporosis in the conditions of the city of Kharkiv were characterized by a decrease in the extent of infestation with the age of the dogs. The most affected were young animals up to 6 months of age, where the extent of infestation was 32.0 %. In dogs aged 6–12 months and 1–3 years, the extent of cystoisosporous infestation decreases to 18.9 and 4.7 %, respectively. Dogs aged 3 to 6 years and older than six were the least affected by cystoisospor oocysts, where the extent of infestation is 4.7 and 2.7 %, respectively. Breed susceptibility to cystoisosprosis is characterized by the most excellent infestation of mixed-breed dogs and purebred animals, where their average infestation was at the level of 22.0 %. Cystoisosporosis was less often diagnosed in dogs of hunting, service, and decorative breeds; the extent of infestation ranged from 10.3 to 14.3 %. In terms of breeds, the highest infestation by protozoan organisms was found in labrador retrievers (extensive infestation 6.0 %), German shepherds (3.9 %), dachshunds (3.5 %), Yorkshire terriers (3.2 %) and cocker spaniels (2.8 %). The obtained data allow us to take into account the obtained results regarding the age and breed susceptibility of dogs to coccidia of the genus Cystoisospora for timely diagnosis and prevention of cystoisosporosis.
Inovasi Pelayanan Kesehatan Jiwa dari Perspektif New Public Service (Studi Kasus Inovasi AKSI LILIN Kabupaten Lebak)
Authors: Barsei Adhityo Nugraha , Sipahutar Nova Yulanda Putri , Prasetyo Antonius Galih , Listanto Virgiawan 
Published: October 2023

Kondisi kesehatan mental di Indonesia berada pada posisi yang mengkhawatirkan. Hasil riset kesehatan dasar menunjukkan bahwa prevalensi gangguan jiwa berat seperti skizofrenia di Indonesia tinggi tetapi masyarakat kurang menyadari penyebab masalah kesehatan jiwa. Selain itu, orang dengan gangguan jiwa enggan atau menunda pengobatan. Dalam menghadapi berbagai permasalahan tersebut, puskesmas sebagai garda terdepan dalam pelayanan kesehatan jiwa harus melakukan kegiatan yang tidak business as usual. Sebagai respon terhadap masalah kesehatan jiwa tersebut, Puskesmas Mandala Kabupaten Lebak menciptakan dan melaksanakan inovasi pelayanan kesehatan jiwa Aksi Lilin (Ajak, Interaksi, Lindungi, Sayangi dengan Nurani). Penelitian ini bertujuan untuk menjelaskan dan menganalisis syarat implementasi inovasi Aksi Lilin sehingga dapat diimplementasikan dengan efektif. Selanjutnya, penelitian ini menganalisis inovasi Aksi Lilin dari perspektif New Public Service (NPS). Penelitian ini menggunakan metode studi kasus untuk menganalisis implementasi inovasi Aksi Lilin. Insentif, otonomi, kepemimpinan dan budaya, dan enabler adalah komponen syarat untuk berinovasi. Implementasi Aksi Lilin juga menerapkan prinsip NPS melalui partisipasi kader kesehatan jiwa. Studi ini mengungkapkan bahwa partisipasi warga melalui kader kesehatan jiwa sangat penting karena puskesmas memiliki sumber daya yang terbatas dalam memberikan layanan publik. Apalagi kehadiran inovasi Aksi Lilin tidak hanya untuk memenuhi target pelayanan tetapi juga untuk mewujudkan tanggung jawab pemerintah adalah memberikan pelayanan terbaik kepada masyarakat, khususnya kepada kelompok rentan seperti masyarakat dengan gangguan jiwa berat.
PRELP secreted from mural cells protects the function of blood brain barrier through regulation of endothelial cell-cell integrity
Authors: Davaapil Hongorzul , Hopkins Jack , Bonnin Nadia , Papadaki Vasiliki , Leung Alex , Kosuge Hirofumi , Tashima Takumi , Nakakido Makoto , Sekido Ryohei , Tsumoto Kouhei , Sagoo Mandeep S. , Ohnuma Shin-Ichi 
Published: October 2023

Introduction: Proline/arginine-rich end leucine-rich repeat protein (PRELP), is a small secreted proteoglycan expressed by pericytes and vascular smooth muscle cells surrounding the brain vasculature of adult mouse.Methods: We utilised a Prelp knockout (Prelp−/−) mouse model to interrogate vasculature integrity in the brain alongside performing in vitro assays to characterise PRELP application to endothelial cells lines. Our findings were supplemented with RNA expression profiling to elucidate the mechanism of how PRELP maintains neurovasculature function.Results:Prelp−/− mice presented with neuroinflammation and reducedneurovasculature integrity, resulting in IgG and dextran leakage in the cerebellum and cortex. Histological analysis of Prelp−/− mice revealed reducedcell-cell integrity of the blood brain barrier, capillary attachment of pericytes andastrocyte end-feet. RNA-sequencing analysis found that cell-cell adhesion andinflammation are affected in Prelp−/− mice and gene ontology analysis as well as gene set enrichment analysis demonstrated that inflammation related processes and adhesion related processes such as epithelial-mesenchymal transition and apical junctions were significantly affected, suggesting PRELP is a regulator of cell-cell adhesion. Immunofluorescence analysis showed that adhesion junction protein expression levels of cadherin, claudin-5, and ZO-1, was suppressed in Prelp−/− mice neurovasculature. Additionally, in vitro studies revealed that PRELP application to endothelial cells enhances cell-cell integrity, induces mesenchymal-endothelial transition and inhibits TGF-β mediated damage to cell-cell adhesion.Discussion: Our study indicates that PRELP is a novel endogenous secreted regulator of neurovasculature integrity and that PRELP application may be a potential treatment for diseases associated with neurovascular damage.
Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of a novel ST3355 ESBL-producing hypervirulent Klebsiella pneumoniae isolate
Authors: Zhou Yi , Cheng Yue , Ma Tianyou , Wang Jun , Li Shaoru , Wang Jingdan , Han Lei , Hou Xinyao , Ma Xinxin , Jiang Sijin , Li Pu , Lv Jia , Han Bei , Da Rong 
Published: October 2023

IntroductionAn extended-spectrum beta-lactamase (ESBL)-hypervirulent Klebsiella pneumoniae (HvKP) strain HKE9 was isolated from the blood in an outpatient.MethodsThe effect of the global regulatory factor RpoS on antimicrobial resistance, pathogenicity, and environmental adaptability was elucidated.ResultsHKE9 is a novel ST3355 (K20/O2a) hypervirulent strain with a positive string test and resistant to cephems except cefotetan. It has a genome size of 5.6M, including two plasmids. CTX-M-15 was found in plasmid 2, and only ompk37 was found in the chromosome. HKE9 could produce bacterial siderophores, and genes of enterobactin, yersiniabactin, aerobactin, and salmochelin have been retrieved in the genome. As a global regulatory factor, knockout of rpoS did not change antimicrobial resistance or hemolytic phenotype while increasing the virulence to Galleria mellonella larvae and showing higher viscosity. Moreover, rpoS knockout can increase bacterial competitiveness and cell adhesion ability. Interestingly, HKE9-M-rpoS decreased resistance to acidic pH, high osmotic pressure, heat shock, and ultraviolet and became sensitive to disinfectants (H2O2, alcohol, and sodium hypochlorite). Although there were 13 Type 6 secretion system (T6SS) core genes divided into two segments with tle1 between segments in the chromosome, transcriptomic analysis showed that rpoS negatively regulated T4SS located on plasmid 2, type 1, and type 3 fimbriae and positively regulate genes responsible for acidic response, hyperosmotic pressure, heat shock, oxidative stress, alcohol and hypochlorous acid metabolism, and quorum sensing.DiscussionHere, this novel ST3355 ESBL-HvKP strain HKE9 may spread via various clonal types. The important regulation effect of rpoS is the enhanced tolerance and resistance to environmental stress and disinfectants, which may be at the cost of reducing virulence and regulated by T4SS.
Cardiac decompensation of patients before transcatheter aortic valve implantation—clinical presentation, responsiveness to associated medication, and prognosis
Authors: Fischer-Rasokat Ulrich , Renker Matthias , Charitos Efstratios I. , Strunk Christopher , Treiber Julia , Rolf Andreas , Weferling Maren , Choi Yeong-Hoon , Hamm Christian W. , Kim Won-Keun 
Published: October 2023

AimsCardiac decompensation (CD) in patients with aortic stenosis is a “red flag” for future adverse events. We classified patients undergoing transcatheter aortic valve implantation (TAVI) into those with acute, prior, or no prior CD at the timepoint of TAVI and compared their clinical presentation, prognosis, and effects of the prescribed medication during follow-up.MethodsRetrospective analysis of patients of one center fulfilling the criteria of 30-day device success after transfemoral TAVI.ResultsFrom those patients with no CD (n = 1,985) ranging to those with prior CD (n = 497) and to those with acute CD (n = 87), we observed a stepwise increase in the proportion of patients in poor clinical condition, NYHA class III/IV, low psoas muscle area, fluid overload (rales, oedema, pleural effusion), reduced ejection fraction, renal insufficiency, and anemia. More diuretics but less renin-angiotensin system inhibitors (ACEI/ARB) were prescribed for patients with acute CD compared to other groups. Prior CD (hazard ratio and 95% CI 1.40; 1.02–1.91) and acute CD (1.72; 1.01–2.91), a reduced general condition (1.53; 1.06–2.20), fluid overload (1.54;1.14–2.08), atrial fibrillation (1.76; 1.32–2.33), and anemia (1.43;1.08–1.89) emerged as strong independent predictors of one-year mortality. In all three classes of CD, prescribing of ACEI/ARB was associated with a substantial improvement of survival.ConclusionsThe clinical presentation of (acute or prior) cardiac decompensation in patients with AS overlapped substantially with that of patients with classical signs of heart failure. Our results may support an early treatment strategy in patients with left ventricular dysfuntion before clinical signs of congestion are manifest. Moreover, these patients require intensive medical attention after TAVI.
Identifying faculty opinions about implementing an online, non-thesis master’s degree
Authors: Maynard Rebekah C. I. , Pennisi Svoboda V. , Lombardini Leonardo 
Published: October 2023

IntroductionAlthough distance education is a growing field, there are benefits and challenges in creating and implementing new online degrees. Faculty play a critical role in forming new online programs and hold differing perceptions about online learning. The purpose of this research was to identify faculty opinions about implementing an online, non-thesis master’s degree as an alternative to the existing thesis-based degree.MethodsIn this study, 17 faculty members in the field of horticulture were surveyed at a major university in the southeastern United States. Q methodology was selected as the research tool to identify the primary opinions faculty held about an online, non-thesis master’s degree. Once participants were surveyed, factor analysis was used to reduce the responses into three perspectives.ResultsThree distinct perspectives were summarized as: (A) in-person instruction is more effective than online education, (B) online programs increase accessibility to graduate degrees, and (C) successful online programs require independent learners. Participants holding the first perspective believed an online program would not benefit student learning and would increase the workload of faculty. Participants with perspectives B and C agreed that an online program would be beneficial in reaching a broader audience of students. Although perspective C placed a high importance on independent learning, perspectives A and B indicated engaging with students was critical to student learning.DiscussionAll factors agreed an online degree would help our department reach non-research-oriented students. However, there was discrepancy in faculty willingness to support the program. Based on the identified faculty perspectives, it is believed that an online, non-thesis master’s would be successful if faculty who are willing to participate in the online degree were trained to be effective online educators and if they encouraged students to hold a deeper level of engagement with the content.
Low BMI, blood calcium and vitamin D, kyphosis time, and outdoor activity time are independent risk factors for osteoporosis in postmenopausal women
Authors: Tang Guo , Feng Long , Pei Yu , Gu Zhaoyan , Chen Tingting , Feng Zeguo 
Published: October 2023

AimTo explore the risk factors of osteoporosis in postmenopausal women in China.MethodThis study collected all patient data from January 2014 to December 2015. Basic information and questionnaires were collected from 524 postmenopausal women in Sanya and Hainan Province. The questionnaire was administered to the enrolled participants by endocrinologists. Biochemical parameters were measured using fasting blood samples, and bone density was measured by dual energy X-ray absorptiometry at the department of radiology of Hainan hospital, PLA General Hospital. Participants with an R-value of ≤-2.5 were diagnosed with osteoporosis. After deleting missing values for each factor, 334 participants were divided into the osteoporosis (n=35) and non-osteoporosis (n=299) groups according to the R-values.ResultsThe participants had a median age of 60.8 years (range: 44–94 years). Among the 334 postmenopausal women included in this study, 35 (10.5%) were diagnosed with osteoporosis. Univariate analysis showed statistically significant differences in age, BMI, type of work, alkaline phosphatase, years of smoking, blood calcium levels, kyphosis, fracture, and asthma between the two groups (P&lt;0.05). In addition, multivariate logistic analysis showed that age (odds ratio [OR]: 1.185, 95% confidence interval [CI]: 1.085–1.293, P&lt;0.001) and kyphosis times (OR:1.468, 95% CI: 1.076–2.001, P=0.015) were positively correlated with postmenopausal osteoporosis, whereas BMI (OR: 0.717, 95% CI: 0.617–0.832, P&lt;0.001), blood calcium levels (OR: 0.920, 95% CI: 0.854-0.991, P=0.027), vitamin D levels (OR: 0.787, 95% CI: 0.674–0.918, P=0.002), and outdoor activity time (OR: 0.556, 95% CI: 0.338-0.915, P=0.021) were negatively correlated with postmenopausal osteoporosis.ConclusionLow BMI, blood calcium and vitamin D levels, kyphosis time, and outdoor activity time are independent risk factors for osteoporosis in postmenopausal women.
Initial effects of crop tree release and traditional thinning on productivity and carbon storage of Cunninghamia lanceolata plantation
Authors: Zou Xianhua , Zheng Ziyang , Yang Chutong , Yang Mengjia , Guo Zhijuan , Wang Yongxin , Huang Zhijun , Zhu Liqin , Xu Liqing , Lin Kaimin 
Published: October 2023

IntroductionThinning has a crucial impact on the carbon dynamics of forest ecosystems. The inconsistencies about the effects of thinning on carbon stocks in previous studies may be attributed to different thinning plans that designed to achieve different management goals.MethodsThis study focuses on Cunninghamia lanceolata as the research object and investigates three treatments: crop tree release (CTR), traditional thinning (TM), and no thinning (CK). The different thinning methods (CTR and TM) had different management objectives and selection of crop trees principle, although with the same thinning density. The short-term effects of CTR and TM treatments on tree growth and carbon storage in Cunninghamia lanceolata plantations were analyzed and compared.ResultsBoth CTR and TM were beneficial to the growth of individual trees when compared to CK, with CTR being more beneficial. The average annual increments in DBH, TH, and volume of a single tree in CTR increased by 62.50%, 61.29%, and 74.07% higher than CK, respectively. So CTR was the best for large-diameter timber yield, which was 77.40% higher than CK, whereas CK had a higher proportion of medium- and small-diameter timber yield. However, CTR had an insignificant short-term boosting effect on stand volume, and its timber volume was considerably higher than that in TM treatment, but not as high as in CK. Therefore, the carbon storage in the tree layer, litter, and different soil layers under different treatments showed that CTR and TM were significantly lower than CK. And the total carbon storage in the three treatments showed that CK &gt; CTR &gt; TM, with CTR showing a 13.07% higher than TM. On the contrary, thinning could effectively improve understory vegetation carbon storage. The carbon storage in the herb and shrub layers under CTR increased by 47.77% and 183.44%, respectively, compared to CK.DiscussionAlthough thinning could significantly promote the growth of individual trees and understory vegetation, their total carbon storage in the short term was lower than that in CK. CTR showed a higher carbon sequestration and sink capacity than TM. It suggests that CTR, as a special type of thinning method, should be encouraged to selected because it has the chance to achieve a faster carbon sequestration rate than traditional thinning and it also can produce higher-quality timber.
Local glucocorticoid synthesis regulates house dust mite-induced airway hypersensitivity in mice
Authors: Merk Verena M. , Phan Truong San , Wiedmann Alice , Hardy Rowan S. , Lavery Gareth G. , Brunner Thomas 
Published: October 2023

BackgroundExtra-adrenal glucocorticoid (GC) synthesis at epithelial barriers, such as skin and intestine, has been shown to be important in the local regulation of inflammation. However, the role of local GC synthesis in the lung is less well studied. Based on previous studies and the uncontentious efficacy of corticosteroid therapy in asthma patients, we here investigated the role of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1/Hsd11b1)-dependent local GC reactivation in the regulation of allergic airway inflammation.MethodsAirway inflammation in Hsd11b1-deficient and C57BL/6 wild type mice was analyzed after injection of lipopolysaccharide (LPS) and anti-CD3 antibody, and in acute and chronic models of airway hypersensitivity induced by house dust mite (HDM) extract. The role of 11β-HSD1 in normal and inflammatory conditions was assessed by high dimensional flow cytometry, histological staining, RT-qPCR analysis, ex vivo tissue cultures, GC-bioassays and protein detection by ELISA and immunoblotting.ResultsHere we show that lung tissue from Hsd11b1-deficient mice synthesized significantly less GC ex vivo compared with wild type animals in response to immune cell stimulation. We further observed a drastically aggravated phenotype in Hsd11b1-deficient mice treated with HDM extract compared to wild type animals. Besides eosinophilic infiltration, Hsd11b1-deficient mice exhibited aggravated neutrophilic infiltration caused by a strong Th17-type immune response.ConclusionWe propose an important role of 11β-HSD1 and local GC in regulating Th17-type rather than Th2-type immune responses in HDM-induced airway hypersensitivity in mice by potentially controlling Toll-like receptor 4 (TLR4) signaling and cytokine/chemokine secretion by airway epithelial cells.