bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of focal cortical cooling on somatosensory evoked potentials in rats
Authors: Gotoh Mizuho , Dezawa Shinnosuke , Takashima Ichiro , Yamamoto Shinya 
Published: December 2023

AbstractAlthough the focal brain cooling technique is widely used to examine brain function, the effects of cortical temperature at various levels on sensory information processing and neural mechanisms remain underexplored. To elucidate the mechanisms of temperature modulation in somatosensory processing, this study aimed to examine how P1 and N1 deflections of somatosensory evoked potentials (SEPs) depend on cortical temperature and how excitatory and inhibitory inputs contribute to this temperature dependency. SEPs were generated through electrical stimulation of the contralateral forepaw in anesthetized rats. The SEPs were recorded while cortical temperatures were altered between 17–38 °C either without any antagonists, with a gamma-aminobutyric acid type A (GABAA) receptor antagonist (gabazine), with aminomethylphosphonic acid (AMPA) receptor antagonist (NBQX), or withN-Methyl-D-aspartic acid (NMDA) receptor antagonist ([R]-CPP). The effects of different gabazine concentrations (0, 1, and 10 µM) were examined in the 35–38 °C range. The P1/N1 amplitudes and their peak-to-peak differences plotted against cortical temperature showed an inverted U relationship with a maximum at approximately 27.5 °C when no antagonists were administered. The negative correlation between these amplitudes and temperatures of ≥27.5 °C plateaued after gabazine administration, which occurred progressively as the gabazine concentration increased. In contrast, the correlation remained negative after the administration of NBQX and (R)-CPP. These results suggest that GABAergic inhibitory inputs contribute to the negative correlation between SEP amplitude and cortical temperature around the physiological cortical temperature.HighlightsFocal cortical cooling altered somatosensory evoked potentials (SEPs).SEP amplitude was negatively correlated with cortical temperatures of 27.5−38.0 °C.GABAAR but not AMPAR nor NMDAR antagonists eliminated the negative correlation.GABAergic signaling is involved in the temperature dependency of SEPs.
Adaptation of a commercial NAD quantification kit to assay the base exchange activity of SARM1
Authors: Cirilli Ilenia , Amici Adolfo , Gilley Jonathan , Coleman Michael P. , Orsomando Giuseppe 
Published: December 2023

ABSTRACTHere we report an adapted protocol using the Promega NAD/NADH-Glo™ Assay kit. The assay normally allows quantification of trace amounts of both oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) by enzymatic cycling, but we now show that the NAD analog 3- acetylpyridine adenine dinucleotide (AcPyrAD) also acts as a substrate. In fact, AcPyrAD generates amplification signals of larger amplitude than those obtained with NAD. We exploited this finding to devise and validate a novel method for assaying the base exchange activity of SARM1 in reactions containing NAD and an excess of the free base 3-acetylpyridine (AcPyr), where AcPyrAD is the product. We also propose an application of this method based on competition between AcPyr and other free bases to rank their preference for SARM1. This has significant advantages over traditional methods for assaying SARM1 base exchange as it is rapid, sensitive, cost-effective, and easily scalable. This could represent a useful tool given current interest in the role of SARM1 base exchange in programmed axon death and related human disorders. It may also be applicable to other multifunctional NAD glycohydrolases (EC 3.2.2.6) that possess similar base exchange activity.
Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest inKomagataella phaffii
Authors: Albacar Marcel , Casamayor Antonio , Ariño Joaquín 
Published: December 2023

ABSTRACTBackgroundThe yeastKomagataella phaffiihas become a very popular host for heterologous protein expression, very often based on the use of theAOX1promoter, which becomes activated when cells are grown with methanol as a carbon source. However, the use of methanol in industrial settings is not devoid of problems, and therefore, the search for alternative expression methods has become a priority in the last few years.ResultsWe recently reported that moderate alkalinization of the medium triggers a fast and wide transcriptional response inK. phaffii. Here, we present the utilization of three alkaline pH-responsive promoters (pTSA1, pHSP12and pPHO89) to drive the expression of a secreted phytase enzyme by simply shifting the pH of the medium to 8.0. These promoters offer a wide range of strengths, and the production of phytase could be modulated by adjusting the pH to specific values. TheTSA1andPHO89promoters offered exquisite regulation, with virtually no enzyme production at acidic pH, while limitation of Pi in the medium further potentiated alkaline pH-driven phytase expression from thePHO89promoter. An evolved strain based on this promoter was able to produce twice as much phytase as the reference pAOX1-based strain. Functional mapping of theTSA1andHSP12promoters suggests that both contain at least two alkaline pH-sensitive regulatory regions.ConclusionsOur work shows that the use of alkaline pH-regulatable promoters could be a useful alternative to methanol-based expression systems, offering advantages in terms of simplicity, safety and economy.
Twitter bots, democratic deliberation and social accountability: the case of #OccupyWallStreet
Authors: Neu Dean , Saxton Gregory D. 
Published: December 2023

PurposeThis study is motivated to provide a theoretically informed, data-driven assessment of the consequences associated with the participation of non-human bots in social accountability movements; specifically, the anti-inequality/anti-corporate #OccupyWallStreet conversation stream on Twitter.Design/methodology/approachA latent Dirichlet allocation (LDA) topic modeling approach as well as XGBoost machine learning algorithms are applied to a dataset of 9.2 million #OccupyWallStreet tweets in order to analyze not only how the speech patterns of bots differ from other participants but also how bot participation impacts the trajectory of the aggregate social accountability conversation stream. The authors consider two research questions: (1) do bots speak differently than non-bots and (2) does bot participation influence the conversation stream.FindingsThe results indicate that bots do speak differently than non-bots and that bots exert both weak form and strong form influence. Bots also steadily become more prevalent. At the same time, the results show that bots also learn from and adapt their speaking patterns to emphasize the topics that are important to non-bots and that non-bots continue to speak about their initial topics.Research limitations/implicationsThese findings help improve understanding of the consequences of bot participation within social media-based democratic dialogic processes. The analyses also raise important questions about the increasing importance of apparently nonhuman actors within different spheres of social life.Originality/valueThe current study is the first, to the authors’ knowledge, that uses a theoretically informed Big Data approach to simultaneously consider the micro details and aggregate consequences of bot participation within social media-based dialogic social accountability processes.
Thermal contact conductance model of rough surfaces with inclination based on three-dimensional fractal theory
Authors: Sun Xianguang 
Published: December 2023

Purpose
The purpose of this study is to establish a thermal contact conductance model of rough surfaces with inclination based on three-dimensional fractal theory.

Design/methodology/approach
The effects of contact load, inclination angle, fractal dimensional and fractal roughness on thermal contact conductance of rough surfaces were studied using numerical simulation.

Findings
The results show that the thermal contact conductance of the rough surface increases with the increase of contact load and fractal dimension and decreases with the increase of fractal roughness and inclination angle. The inclination angle of the rough surface has an important influence on the thermal contact conductance of the rough, and it is a factor that cannot be ignored in the study of the thermal contact conductance of rough surfaces.

Originality/value
A thermal contact conductance model of rough surfaces with inclination based on three-dimensional fractal theory was established in this study. The achievements of this study provide some theoretical basis for the investigation of the thermal contact conductance of rough surfaces.
A systematic review on work from home (WFH) and libraries: service delivery, tools used, challenges and opportunities
Authors: Siddique Nadeem , Safdar Muhammad , Ahmad Adeel , Khan Muhammad Ajmal 
Published: December 2023

Purpose
The paper aims to systematically collect and review the literature on work from home (WFH) and libraries. Another objective of the review is to identify the services professionals offered while WFH, tools used, challenges and opportunities in this connection.

Design/methodology/approach
The study consulted four databases, namely, Web of Science, Scopus, Google Scholar and Library, Information Science and Technology Abstracts, to collect and review the literature on the topic. Preferred Reporting Items for Systematic Reviews and Meta-Analyses guideline was used to select the eligible studies for this review. A total of 27 studies out of 1,571 were considered eligible for the study. The authors also consulted Boynton and Greenhalgh (2004) guide for assessing the quality of the reviewed research. All types of studies and except books were selected for the review. However, the researchers did not apply the time limitation.

Findings
The research identified that libraries provided various services, including Mystery Box, live chat and 24-hour service, using different tools, including MS Teams, Zoom and Google Meet, while WFH. The study shared different challenges and opportunities identified from the reviewed literature, for libraries.

Originality/value
To the best of the authors’ knowledge, this is the first systematic review conducted to systematically collect and review the literature produced on WFH in terms of libraries regarding offered services, tools used to serve the users, challenges and opportunities. The review filled the literature gap and shared implications for library professionals and related stakeholders.
Do we really make a difference? A case study on the value of taught environmental sustainability postgraduate programmes within geography
Authors: Rodway-Dyer Sue , Barr Stewart 
Published: December 2023

Purpose
The purpose of this research was to discover the impacts of taught environmental sustainability-focused geography postgraduate programmes on student attitudes, behaviours and practices in relation to environmental awareness within two research-intensive universities in the UK.

Design/methodology/approach
A case study involved online surveys to measure environmental attitudes, behaviours and practices at the start and end of four taught geography postgraduate programmes.

Findings
There was widespread attitudinal change and an increasing prioritisation of environmental issues reported among participants after they had completed their programme. However, behavioural change was limited, and there was little evidence of greater awareness being translated into changed practices. The learning benefits included a greater focus on interdisciplinarity, holistic thinking and critical self-reflection.

Practical implications
The findings demonstrate that postgraduate taught (PGT) programmes in sustainability improve student awareness and concern about environmental issues but do not necessarily lead to widespread behavioural change. This raises questions for programme convenors about how education for sustainability can be truly transformational and avoid leading students to develop eco-anxieties over the scale of change required.

Originality/value
There is a lack of research engaging with students on PGT programmes, especially in understanding their impacts on environmental attitudes, behaviours and practices. The research provides an evidence base for understanding the effects of PGT programmes in challenging student values, attitudes and practices and by implication knowledge transfer post-graduation, with the potential to help protect the environment and identify ways of living better with the ever-changing planet.
Constructing Spanish smart destinations: a new guide for the tourism industry
Authors: Florido-Benítez Lázaro 
Published: December 2023

Purpose
The purpose of this study is to analyse Benidorm, San Sebastián, Gijón, Málaga, Tenerife Island and Santander smart tourist destinations (STDs) as a touristic model and example to follow by other destinations in Spain and all over the world.

Design/methodology/approach
To fulfil the stated objective, this study follows several phases that introduce and classify a set of measures implemented by the six Spanish smart destinations to be designed as a STD.

Findings
Findings suggest that being a STD requires a high cost, and this is only accessible to big destinations with enough resources. Of the 50 Spanish provinces, eight are STDs, and these are localised in coastal areas. Obviously, this challenge is not within the reach of any Spanish city. Moreover, findings of the current study prove that the six Spanish smart destinations have a good air accessibility through their six airports, but the accessibility in a STD is not just physical; this must provide digital accessibility to tourists through destination marketing organisation’s website and app that will supply them with information on a wide range of services, including accommodations, tourist attractions, restaurants, public transport, museums and monuments’ locations, amongst many others.

Originality/value
From a resident point of view, a STD cannot be only focused on a technological and tourism context; a STD also requires knowing and meeting the needs of local residents and having a voice in decision-making processes. Hence, this study shows a new perspective on STDs that will benefit the literature on STDs.
Key success factors for stability of asymmetric technological collaborations: a bionic engineering approach
Authors: Ghazinoory Sepehr , Aghaei Parvaneh 
Published: December 2023

Purpose
This study aims to investigate the importance and effect of asymmetric technological collaborations’ key success factors in developing countries. The number of collaborations between large enterprises and SMEs, known as asymmetric technological collaborations (ATC) is growing considerably. But this asymmetry in itself can increase the number and intensity of collaboration challenges. So far, limited studies have been conducted on the stability of ATCs, and most of them have been in the context of developed countries. Meanwhile, studying the strength and stability of collaboration in the nano industry with growing market value and increasing newcomers is of particular importance.

Design/methodology/approach
Here, with bionic engineering approach, we used chemistry for the first time to identify the main stability factors of ATCs and build our hypotheses and research model. To this end, we introduced the factors affecting the stability of the dative chemical bond as a bionic counterpart of corporate venture capital (CVC), which is a type of ATC, and proposed 4 hypotheses. We used structural equation modeling (SEM) with partial least squares (PLS) method to examine the hypothesized relationships.

Findings
The analysis of survey questionnaire data from 26 asymmetric collaborations in Iran’s nanotechnology industry shows that “learning of the acceptor company” with a negative effect, “network ties” and “development of the collaboration host region” with a positive effect and “diversity in the collaboration portfolio” with an inverted U-shaped effect are the most influential factors in the stability and continuity of CVCs, respectively.

Originality/value
The findings of this research can be the beginning of a broad path leading to exploring and getting inspiration from chemistry to analyze management issues.
Full Engagement with the NHS in an integrated age: reflections on past endeavours (the Wanless Report) and current challenges (the anti-vaxxer movement)
Authors: Manthorpe Jill , Iliffe Steve , Bourne Richard 
Published: December 2023

PurposeIt is over 20 years since the publication of the Wanless Report, “Securing our Future Health: Taking a Long-Term View”. The Wanless Report argued that the National Health Service (NHS) would survive in its current form only if the population became “fully engaged” with it.Design/methodology/approachIn this discussion paper, the authors explored what “fully engaged” meant to Wanless, what it might mean now (allowing for the impact of the anti-vaxxer movement) and what policymakers could do to enhance public engagement.FindingsAlthough the Wanless Report neatly fitted into other long-term thinking about the NHS, it was unique in that it built economic models to predict the costs and impact of different patterns of NHS performance. Wanless predicted that people’s poor levels of health would put considerable pressure on the NHS. This pressure could swamp efforts to meet healthcare targets and improve health outcomes, despite its sizeable investment of money. Wanless set out three possible scenarios for public engagement with the NHS: solid progress, slow uptake and fully engaged.Practical implicationsThe authors pose questions for policymakers and practitioners. Would a reboot of the Wanless approach be worth the effort for policymakers? If yes, how would it differ from the original? The NHS faces the whole of society; could it be the vehicle for engaging the anti-vaxxer public with the truthfulness of medical science, and will it be this, that is, Wanless' enduring legacy?Originality/valueThe exploration of the Wanless Report is complicated (at least for the time being) by the rise of the anti-vaxxer movement’s resistance to health promotion and mistrust of part of the NHS.
A COVID replication and extension of firms’ resilience to supply chain disruptions
Authors: Inman R. Anthony , Green Kenneth W. , Roberts Matthew D. 
Published: December 2023

Purpose
The purpose is to replicate and extend Ambulkar et al.’s (2015) work testing resource reconfiguration as a mediator of the supply chain disruption/firm resilience relationship and testing risk management infrastructure as a moderator. This study extends the work of Ambulkar in that it uses analysis of survey data gathered from manufacturing firms during an actual disruption event (COVID-19). The previous work is also in extended in that the authors include a pandemic disruption impact variable and supply chain performance is an expanded model.

Design/methodology/approach
Partial least squares structural equation modeling techniques were used to analyze data gathered from 184 US manufacturing managers during the height (Summer 2021) of the COVID-19 pandemic.

Findings
Two of four of Ambulkars et al.’s (2015) hypotheses were confirmed as relevant to firm resilience during the pandemic while two were not confirmed. Results also show that supply chain disruption orientation, risk management infrastructure and resource reconfiguration combine to improve firm resilience, which in turn improves supply chain performance while mitigating the disruption impact of COVID-19.

Originality/value
Previous work is replicated and extended, using data from an actual disruption event (COVID-19). This study presents a more comprehensive model using a newly developed and validated scale to measure pandemic impact and including supply chain performance.
An iron fist in a velvet glove: The cooperation of a novel pyoverdine from Pseudomonas donghuensisP482 with 7‐hydroxytropolone is pivotal for its antibacterial activity
Authors: Jafra Sylwia , Jabłońska Magdalena , Maciąg Tomasz , Matuszewska Marta , Borowicz Marcin , Prusiński Michał , Żmudzińska Wioletta , Thiel Marcel , Czaplewska Paulina , Krzyżanowska Dorota M. , Czajkowski Robert 
Published: December 2023

AbstractPseudomonas donghuensis P482 exhibits broad antimicrobial activity against phytopathogens, including the soft rot bacteria of the Dickeya genus. Here, we report that under limited nutrient availability, the antibacterial activity of P. donghuensis P482 against Dickeya solani requires the reciprocal action of two iron scavengers: 7‐hydroxytropolone (7‐HT) and a newly characterized pyoverdine (PVDP482) and is quenched in the iron‐augmented environment. Further, we show that the biosynthesis of pyoverdine and 7‐HT is metabolically coordinated, and the functional BV82_4709 gene involved in 7‐HT synthesis is pivotal for expressing the BV82_3755 gene, essential for pyoverdine biosynthesis and vice versa. The synthesis of both scavengers is under the control of Gac/Rsm, but only PVD is controlled by Fur. The isoelectric focusing profile of the P482 siderophore differs from that of the other Pseudomonas spp. tested. This finding led to the unveiling of the chemical structure of the new pyoverdine PVDP482. To summarize, the antibacterial activity of P. donghuensis P482 is attributed to 7‐HT and PVDP482 varies depending on the nutrient and iron availability, highlighting the importance of these factors in the competition between P482 and D. solani.
Study of the influence of nanoscale porosity on the microbial electroactivity between expanded graphite electrodes and Geobacter sulfurreducens biofilms
Authors: Ramírez‐Moreno M. , Berenguer R. , Ortiz J. M. , Esteve‐Núñez A. 
Published: December 2023

AbstractExpanded graphite (EG) electrodes gather several advantages for their utilization in microbial electrochemical technologies (MET). Unfortunately, the low microbial electroactivity makes them non‐practical for implementing them as electrodes. The objective of this work is to explore the enhancement of microbial electroactivity of expanded graphite (commercial PV15) through the generation of nanopores by CO2 treatment. The changes in properties were thoroughly analysed by TG, XRD, Raman, XPS, gas adsorption, SEM and AFM, as well as microbial electroactivity in the presence of Geobacter sulfurreducens. Nanopores remarkably enhance the microbially derived electrical current (60‐fold increase). Given the inaccessibility of micron‐sized bacteria to these nanopores, it is suggested that the electric charge exchanged by electroactive microorganisms might be greatly affected by the capability of the electrode to compensate these charges through ion adsorption. The increased microbial current density produced on activated PV15 opens the possibility of using such materials as promising electrodes in MET.