bluuebunny/crossref_metadata_2025_split huggingface dataset:
Larval development of Fasciola hepatica in experimental infections: variations with populations of Lymnaea truncatula
Authors: Vignoles P. , Dreyfuss G. , Rondelaud D. 
Published: February 2003

AbstractA retrospective study was undertaken on 70 French populations of Lymnaea truncatula experimentally infected with Fasciola hepatica to determine whether or not susceptibility of snails to infection influenced redial and cercarial production. Results were compared with those obtained from two control populations, known for prevalences higher than 60% when experimentally infected with F. hepatica. In the 70 other populations examined, the prevalences ranged from 2 to 75%. In 55 of these populations, where the prevalence was more than 20%, a high proportion (50.1–56.8%) of snails died after cercarial shedding, whereas in the other groups (non-shedding snails with the most differentiated larvae being free cercariae, rediae containing cercariae, immature rediae, or sporocysts, respectively), snail death was significantly less. In 11 populations, where the prevalence values were 5–19%, only 14% of snails died after cercarial shedding, whereas snails with free cercariae, rediae with cercariae, or immature rediae showed significant increases in snail mortality. In the remaining four snail populations, with prevalences of less than 5%, the most differentiated larval forms were only immature rediae and/or sporocysts. Overall, the number of rediae containing cercariae significantly decreased with decreasing prevalence values. The low prevalence of experimental infection in several populations of snails might be explained by the occurrence of natural infections with miracidia originating from a mammalian host other than cattle, and/or by genetic variability in the susceptibility of snails to infection.
Redial growth and cercarial productivity of Fasciola hepatica in three species of young lymnaeid snails
Authors: Vignoles P. , Dreyfuss G. , Rondelaud D. 
Published: February 2003

AbstractExperimental infections of 1-mm high snails using three populations of Lymnaea (L. glabra, L. ovata and L. truncatula) and a cattle strain of Fasciola hepatica miracidia were carried out under laboratory conditions to determine if the snail species had an effect on the number of free rediae, their growth, and cercarial productivity in relation to each redial category (R1a, R1b, R2a, or R2b/R3a). The total number of rediae ranged from 6.4 to 7.5 per snail. The mean body length of rediae varied from 1–1.2 mm (R1a) to 0.3–0.4 mm (R2b/R3a). The width of the intrapharyngeal lumen also varied from 26.0–38.8 μm to 3.0–4.2 μm, respectively. The redial category had a significant effect on both measurements, whereas snail species only had a significant influence on body length. The mean number of cercariae produced by all living rediae at day 49 post-exposure ranged from 63.0 in L. glabra to 87.2 in L. truncatula. In L. ovata and L. truncatula, 55.8% and 58.6% of cercariae, respectively, were produced by R2a rediae, whereas 53.9% of cercariae in L. glabra were formed by the R1b rediae. When young snails were infected with F. hepatica, the species of snail had an effect on the number of living rediae, their length and their cercarial productivity.
Immunization with cathepsin L proteinases CL1 and CL2 secreted by Fasciola hepatica elicit a preferential type 1 response based on IgG2a antibodies in rats
Authors: Bentancor A. , Piacenza L. , Carmona C. 
Published: February 2003

AbstractCathepsin L proteinases (CL1 and CL2), the major components of Fasciola hepatica excretion/secretion products (E/S) are considered potential antigens of a vaccine against fascioliasis. The humoral response elicited by CL1 and CL2 in rats either immunized with the enzymes or infected with F. hepatica has been analysed, examining specific IgE and IgG subclass dynamics. The experiment was continued for 10 weeks and peripheral blood eosinophilia was also determined. Infected rats presented peaks of eosinophilia at weeks 3 and 7 post-infection, while those immunized with CL1 and CL2 were no different from controls. Total IgE in infected rats increased up to week 5, reaching 30 μg -1 in some cases, then decreased slowly and rising again towards the end of the experiment. Determination of specific IgE, carried out in sera previously absorbed with Protein G-Sepharose, reached a peak in infected rats between weeks 2 and 5, depending on the individual. In immunized rats both total and specific IgE levels remained around the pre-immunization values. With regard to the IgG subclass responses to E/S products, in infected rats IgG1 predominated over IgG2a, and the reverse was true in rats immunized with CL1 and CL2 and tested against the respective antigens. In all cases an increase in IgG1 and IgG2a antibody titres was seen, with maximum levels being reached later (weeks 6–7) in infected rats than in immunized ones (weeks 4–5). No IgG2b or IgG2c responses were detected in any of the groups studied.
Larval trematode infections in freshwater snails from the highveld and lowveld areas of Zimbabwe
Authors: Chingwena G. , Mukaratirwa S. , Kristensen T.K. , Chimbari M. 
Published: February 2003

AbstractBetween November 1998 and October 2000, freshwater snails were collected monthly from the highveld and lowveld areas of Zimbabwe to determine the occurrence of larval trematodes. A total of 13,789 snails, representing ten species, were collected from 21 sites and 916 (6.6%) harboured patent trematode infections. Eight morphologically distinguishable types of cercariae were identified. Bulinus tropicus had the highest overall prevalence of infection (13.1%). The echinostome was the most common type of cercaria recovered, contributing 38.2% of all infections. Schistosoma cercariae were recovered mainly from the highveld and comprised 8.0% of all infections. Amphistome cercariae contributed 37.6% of all infections and were recorded from both the highveld and lowveld areas with a peak prevalence occurring during the post-rainy period (March–May). The main intermediate host for amphistomes was B. tropicus. Infections in B. globosus, B. forskalii and Biomphalaria pfeifferi with amphistome cercariae are new records for Zimbabwe.
Helminth communities of two sympatric skinks (Mabuya agilis and Mabuya macrorhyncha ) from two ‘restinga’ habitats in southeastern Brazil
Authors: Vrcibradic D. , Rocha C.F.D. , Bursey C.R. , Vicente J.J. 
Published: February 2003

AbstractThe helminth fauna of two sympatric congeneric skinks (Mabuya agilisandM. macrorhyncha) from two distinct ‘restinga’ habitats (Praia das Neves and Grussaí) in southeastern Brazil were studied, totalling four data sets (sample sizes ranging from 11 to 28). A total of ten helminth species were associated with the skinks:Raillietiellasp.,Paradistomum parvissimum,Pulchrosomoides elegans,Oochoristica ameivae,Hexametra boddaertii,parapharyngodon sceleratus,Physalopteroides venancioi,Physalopterasp., an unidentified acuariid nematode and an unidentified centrorhynchid acanthocephalan. Except forHexametra boddaertii(found only in Grussaí) andPulchrosomoides elegans(found only in Praia das Neves), all helminth species were present at both localities. Half of the helminth species were present only as larvae and, in most cases, appear to represent paratenic parasitism. Overall prevalences of infection were high for both host species in both localities.Mabuya agilistended to have richer and more diverse infracommunities thanM. macrorhyncha. Some parameters of infection by individual helminth species seem to be related to the ecology of eachMabuyaspecies. The parasite faunas were qualitatively very similar among species and/or localities, but quantitative similarities were more varied, due to differential representativeness of individual helminth species among host populations. The helminth communities of both skink species can be classified as non-interactive, being composed of site-specialists and immature stages of non-lizard parasites.
Effect of acanthocephalan parasites on hiding behaviour in two species of shore crabs
Authors: Latham A.D.M. , Poulin R. 
Published: February 2003

AbstractThe effect of acanthocephalan parasites (profilicollis spp.) on the hiding behaviour during low tide of two species of shore crabs (intermediate hosts), Macrophthalmus hirtipes (Brachyura: Ocypodidae) and Hemigrapsus crenulatus (Brachyura: Grapsidae), was examined at Blueskin Bay, South Island, New Zealand. Exposed M. hirtipes were found to have significantly higher infection levels than did hidden conspecifics. This pattern was not observed for H. crenulatus. Mean cystacanth numbers were found to be considerably higher in M. hirtipes than H. crenulatus. Crabs exposed at low tide are at a greater risk of predation by definitive shorebird hosts than are hidden conspecifics. Preferential manipulation of one intermediate host species over another could influence diversity within ecosystems.
Long-term stability in the richness and structure of helminth communities in eels, Anguilla anguilla, in Lough Derg, River Shannon, Ireland
Authors: Kennedy C.R. , Moriarty C. 
Published: February 2003

AbstractA data set on intestinal helminth parasites was collected in the course of an 18 year investigation into the biology of eels in Meelick Bay, Lough Derg, River Shannon. This was used to test two hypotheses relating to the composition and structure of intestinal helminth communities, namely that eels in large rivers do not harbour richer and more diverse communities than those in small rivers but that community composition and structure are more stable over time than in small rivers. The helminth community was species poor, with only six species comprising the component community and a maximum infracommunity richness of three species. The community was overwhelmingly dominated by the acanthocephalan Acanthocephalus lucii, reflecting the importance of its intermediate host Asellus aquaticus in the eels' diet. The remaining helminth species contributed to species richness but made very little contribution to community diversity. Population levels of Acanthocephalus lucii fell and remained low between 1992 and 2000, probably reflecting increased movement of eels from other parts of the lough into Meelick Bay. Diversity values were low, but similar to those reported from other rivers in Britain and Europe. The results provided support for both hypotheses and indicated that in respect of richness, diversity and dominance, the helminth communities of eels in the River Shannon were typical of, and comparable to, those of other large rivers throughout Europe.
Sampling description and procedures used to conduct the North/South Ireland Food Consumption Survey
Authors: Kiely M , Flynn A , Harrington KE , Robson PJ , Cran G 
Published: February 2003

AbstractObjectiveThe purpose of this survey was to establish a database of habitual food and drink consumption in a representative sample of Irish adults, aged 18–64 years. The present paper describes the sampling protocol, response rate and characteristics of the survey population in terms of sex and age groups, seasonality, geographical location, marital status, social class, socio-economic status and education level.DesignA cross-sectional food consumption survey was carried out. In the Republic of Ireland, a nationally representative sample of adults was randomly selected with a validated two-stage clustered design, using the electoral register as the sampling frame. This method produced a self-weighting or ‘epsem’ sample of individuals, where each adult who was registered to vote had an equal opportunity of being selected. Similarly, in Northern Ireland, a two-stage random sampling procedure was used. The sampling frame was the electoral register, and the sample was stratified by urban/rural and by an index of material deprivation, to ensure representation of each sector of the community. The recruitment procedure was the same in the North and South. An introductory letter with an information leaflet was posted to each selected individual and these were followed up by a visit from a fieldworker, who invited participation in the survey.SettingNorthern Ireland and Republic of Ireland between 1997 and 1999.ResultsThe response rate, which is the percentage of the total number of people who completed a 7-day food diary (n = 1379) out of the total eligible sample (n = 2177), was 63%. Non-respondents and dropouts constituted 34% and 3%, respectively, of the total eligible sample. Compared with the most recent census figures available, the sample was generally found to be representative in terms of sex and age group profiles, geographical location, marital status, seasonality, social class, socio-economic group and education level. Data on sex and age group and geographical location were collected from non-respondents for comparison with the survey sample. There were no apparent differences between them.ConclusionThe North/South Ireland Food Consumption Survey has established a Samplino relational database of habitual food and drink consumption, in addition to data on Response rate habitual physical activity, anthropometric measurements, socio-demographic factors, Demographics lifestyle, health status indicators and attitudes, in a nationally representative sample Socio-economic factors of the population of the island of Ireland.
The efficacy and safety of nutritional supplement use in a representative sample of adults in the North/South Ireland Food Consumption Survey
Authors: Kiely M , Flynn A , Harrington KE , Robson PJ , O'Connor N , Hannon EM , O'Brien MM , Bell S , Strain JJ 
Published: February 2003

AbstractObjectiveTo describe the current use of nutritional supplements and their contribution to micronutrient intakes in a representative sample of Irish adults, to evaluate the impact of supplement use on the adequacy of micronutrient intakes and to assess the risk to supplement users of exceeding tolerable upper intake levels (UL).Study design and subjectsFood intake data were collected in 1379 (662 male and 717 female) randomly selected Irish adults aged 18 to 64 years using a 7-day food diary. The current use of nutritional supplements was assessed using a selfadministered questionnaire and respondents entered each supplement as it was consumed into the food diary.ResultsTwenty-three per cent of respondents regularly used nutritional supplements. Twice as many women used supplements as men. The intakes of micronutrients were significantly higher (P < 0.001) in supplement users than in non-users. Micronutrient intakes from food sources were similar in male users and non-users of supplements, but were significantly higher (P < 0.01) in female users, by 3 to 13%, for Fe, Mg, Mn, vitamins C and E and niacin than in non-users. The percentage of female users between 18 and 50 years who had mean Fe intakes below the average requirement (AR) (10 mg) decreased from 50 to 25 when the contribution from supplements was included. The use of supplements reduced the percentage of men who had mean intakes below the AR for Zn from 19 to 13, for riboflavin from 14 to 6 and for vitamin A from 20 to 5, and reduced the percentage of women with intakes below the AR for Ca from 23 to 16 and for riboflavin from 23 to 14. Twenty-one women out of 80 aged between 18 and 50 years, who consumed supplemental folate, achieved the intake of 600 |xg recommended to prevent neural tube defects. Twenty-two per cent of the women who took iron and 15% of the women who took vitamin B$ in supplemental form had mean daily intakes that exceeded that UL for these nutrients. Supplement users did not exceed the UL for the other micronutrients.ConclusionsSupplementation appears to be beneficial in promoting adequate intakes of some micronutrients, particularly Fe and folate in women aged 18–50 years and vitamin A in men. There appears to be little risk to supplement users of experiencing adverse side effects due to excessive intakes of micronutrients.
Analyses of the anthropometric data from the North/South Ireland Food Consumption Survey
Authors: McCarthy SN , Harrington KE , Kiely M , Flynn A , Robson PJ , Livingstone MBE , Gibney MJ 
Published: February 2003

AbstractObjectiveTo obtain measured anthropometric data for weight, height and other parameters not previously measured in the Irish population such as waist and hip circumferences and body composition.DesignA cross-sectional survey. Weight, height, waist circumference, hip circumference and body composition were measured according to standard procedures.SettingNorthern Ireland and the Republic of Ireland, 1997–1999.SubjectsRandom representative sample of 1379 adults aged 18–64 years. Results: With the exception of body fat, all anthropometric values were significantly higher for men than women (P < 0.001). All measurements were significantly higher in the 36–50-year-old age group compared with 18–35 year olds. Height was the exception, which decreased significantly with age (P < 0.05). Weight, height and body mass index (BMI) have increased in Ireland since last measured in 1988 and in 1990. Over the last decade, obesity has increased in men 2.5 fold from 8% to 20% and in women by 1.25 fold from 13% to 16%. Significantly more women have a normal BMI than men (50.4% vs. 33.3%; P < 0.05). Cut-off points for a high waist circumference and high waist-to-hip ratio identified 47% and 33% of the population, respectively, to be at an increased risk for cardiovascular disease. Social class did not have any significant effect on mean BMI. Location of residence influenced BMI but not in any consistent manner. Ex-smokers had a significantly higher BMI than nonsmokers and smokers (P < 0.05).ConclusionsA revision of current recommendations for combating obesity is warranted to improve the health of the Irish population. Further research is needed to identify the factors that have contributed to the dramatic increase in the prevalence of obesity in men over the last decade and have resulted in a higher prevalence of obesity in men than in women.
Increased vitamin A intake in children aged 2–5 years through targeted home-gardens in a rural South African community
Authors: Faber Mieke , Venter Sonja L , Benadé AJ Spinnler 
Published: February 2003

AbstractObjectives:To determine vitamin A intake of children aged 2–5 years in a rural South African community one year after the implementation of a home-based food production programme targeting β-carotene-rich fruits and vegetables.Design:Dietary intake of children aged 2–5 years was determined during a cross-sectional survey before and one year after the implementation of a home-based food production programme.Setting:A low socio-economic rural African community, approximately 60 km north-west of the coastal city of Durban in kwaZulu-Natal, South Africa.Subjects:Children aged 2–5 years (n=100); 50 children from households with home-gardens producing β-carotene fruits and vegetables (project gardens), and 50 children from households without project gardens.Results:As compared with baseline data, there was a significant increase in vitamin A intake in children from households with project gardens as well as in children from households without project gardens. However, children from households with project gardens had a significantly higher vitamin A intake than children from households without project gardens. The increased vitamin A intake in those children from households without project gardens can be attributed to the availability of butternuts in the local shop (as a result of the project), and because the mothers negotiated with project garden mothers to obtain these fruits and vegetables for their children.Conclusion:A home-based food production programme targeting β-carotene-rich fruits and vegetables can lead to an increase in vitamin A intake.
Nutrition transition in Chile: determinants and consequences
Authors: Albala Cecilia , Vio Fernando , Kain Juliana , Uauy Ricardo 
Published: February 2003

AbstractObjectives:The purpose of this study was to analyse the determinants and consequences of the nutrition transition in Chile and describe the related health promotion policies.Design and setting: This is a descriptive, population-based study including data on demographic, diet, nutrition and biomedical related variables. Data came from the Food and Agriculture Organization (FAO), the National Institute of Statistics (INE), the Ministries of Planning, Health and Education surveillance systems, and national surveys.Results:As malnutrition decreased during the 1980s, obesity increased rapidly in all age groups. In adults, currently about 25% of women are obese (body mass index > 30 kgm−2); particularly those from low socio-economic levels. Among pre-schoolers, obesity is now 10% while in 6-year-old children it is 17.5% (weight/height greater than two standard deviations (>2SD) of the World Health Organization reference). Nutritional risk factors are prevalent, diet is changing to a ‘Western diet’ with an increasing fat consumption, and sedentarianism is constant in all groups. High blood pressure (>140/90) is greater than 10% in adults. Diabetes is increasing in urban areas, including in the indigenous population, and more than 40% of adults have a cholesterol level of more than 200mgml−1.Conclusions:Promotion of healthy lifestyles is the main strategy to cope with this situation, particularly changing behaviour in food habits, physical activity and psychosocial factors. Changes in lifestyles will not only allow the prolonged life expectancy to be of better quality, but also will favour a decrease in the morbidity and mortality from chronic diseases, mainly cardiovascular diseases.
The nutrition transition in Cuba in the nineties: an overview
Authors: Rodríguez-Ojea Arturo , Jiménez Santa , Berdasco Antonio , Esquivel Mercedes 
Published: February 2003

AbstractObjective:To describe and analyse the changes in diet, physical activity and body composition of the Cuban population during the 1990s and the health implications of these changes.Design and setting: Data on national food balance and physical activity are from government agencies and the Ministry of Public Health; nation-wide and local representative surveys were used to analyse body composition and leisure activities. Data on morbidity and mortality arefrom the Ministry of Public Health.Results:The collapse of the European socialist countries and the Soviet Union, as well as the reinforced United States' blockade, provoked a sudden shortage of fuel, raw materials, imported foods and essential supplies. Per capita energy availability decreased, physical activity increased, and the prevalence of obesity decreased. Nutrition deficiencies were observed in the early 1990s, while the trend of morbidity from non-communicable disease continued to increase. The nutrition transition characteristics following the economic recovery in 1995–1996 resembled those of the 1980s because of the increased food availability, decreased physical activity and increased obesity prevalence.Conclusions:Programmes to deal with the complex situation generated a response in a remarkably short time. Undesirable changes in diet composition and the reduction of physical activity constitute a challenge in the current post-critical stage that must be prevented.