bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prototype of a nanostructured multi-epitope vaccine for the control of Piscirickettsiosis: Proof-of concept in salmonid cells
Authors: Paula  
Published: February 2025

Abstract

The article focuses on the development of a nanostructured multi-epitope vaccine prototype to control Piscirickettsiosis, a serious bacterial infection caused by Piscirickettsia salmonis in salmonids.
Piscirickettsiosis, one of the main causes of mortality in Chilean aquaculture, generates significant economic losses and extensive use of antibiotics, which represents risks to environmental and public health. Despite the existence of vaccines, their efficacy remains limited, especially under field conditions, where the genetic variability of the pathogen and other external factors compromise immunological protection. This research applies reverse vaccinology to identify specific antigenic epitopes of P. salmonis, particularly of the LF-89 and EM-90 genogroups, which are predominant in Chilean aquaculture. The study includes the design and production of four nanoparticles (NPs) with chimeric characteristics, called SkipZ, PulseJ, HopQ and Hoptech, derived from P. salmonis epitopes. These nanoparticles were expressed in Escherichia coli and purified for further immunogenic evaluation. The research analyses the uptake of these nanostructures by salmonid RTS-11 macrophage cells and their ability to induce antigen presentation and pro-inflammatory responses. The results show that the nanoparticles, especially SkipZ and HopQ, effectively stimulate the expression of key markers involved in antigen presentation, such as MHC-II, CD83 and CD86, as well as pro-inflammatory cytokines such as IL-1β and TNF-α, in a dose-dependent manner. These findings suggest that the selected epitopes are capable of enhancing immune responses in salmonid cells. This multi-epitope vaccine approach seeks to offer a more specific and effective strategy to control Piscirickettsiosis, potentially reducing the dependence on antibiotics and improving the long-term protection of salmonid populations. The work highlights the potential of using nanoparticle-based vaccines to induce robust cellular immunity, critical to combat intracellular pathogens such as P. salmonis. This proof-of-concept study paves the way for the development and optimization of vaccines tailored to the pathogen-specific genetic diversity in aquaculture environments.
Trade Policy Uncertainty and Inflation Tax Dynamics: Is the 'Most Beautiful Word' a Tax in Disguise?
Authors: Yayi Constant L 
Published: February 2025

Abstract

Does trade policy uncertainty (TPU) erode consumers’ purchasing power? This paper addresses this question using a TPU-augmented structural New Keynesian Phillips Curve model and panel data from 44 countries spanning 1995–2020. We employ two complementary metrics of TPU: tariff overhang and news-based measures of trade uncertainty. Our results show that TPU has a positive and significant effect on inflation tax, with a one log unit increase in tariff overhang leading to a 4%–6% rise in inflation tax. The findings also reveal that trade structure plays a critical role in amplifying TPU’s effects, particularly in import-reliant economies where higher import prices exacerbate inflationary pressures. Furthermore, we note that exchange rate adjustments mitigate the inflationary impact of TPU under specific conditions, highlighting the interplay between trade and monetary dynamics. Overall, we find that TPU’s effect on inflation tax depends on exchange rate volatility, import prices, and their interaction, underscoring the complex mechanisms at play. These results emphasize the need for integrated policies that address both trade and exchange rate uncertainties to safeguard consumers’ purchasing power.
JEL Classification : D80, E3, F1,.F13
Parents’ perceived barriers and enablers to providing optimal infant oral care
Authors: Walsh Olivia , Chauhan Amrit , Trinh My-Van , Lin Clare , Marshall Sarah , Gray-Burrows Kara A , Silva Mihiri 
Published: February 2025

Abstract

Background
Early childhood caries (tooth decay) can adversely affect child growth, development and well-being and is a leading cause of preventable hospitalisation for pre-school aged children. This necessitates the introduction of preventive measures in infancy, including twice daily toothbrushing and timely dental visits. This study explored the barriers and enablers parents face in providing optimal oral care for their young children.
Methods
We interviewed Australian parents with 0-36-month-old children about two key behaviours related to their child’s oral health: 1) the timing of first dental visit and 2) twice daily toothbrushing. Parents were recruited via social media advertising and all interviews were conducted online via Zoom. Interviews were based on a semi-structured interview guide mapped to the Theoretical Domains Framework (TDF). All interviews were audio recorded and transcribed. Data was coded to the TDF, summarised, and categorised as a barrier or enabler before being grouped into themes and sub-themes using framework analysis.
Results
Fifteen interviews were completed between May 2022 – May 2023. Thirteen of the 14 TDF domains were represented in the data. The three most dominant TDF domains across the dataset were social influences, environmental context and resources, and knowledge. Four themes were developed from the data: 1) Conflict, 2) Family and social norms, 3) Wanting a positive oral health experience, and 4) Uncertainty. These themes represent both barriers and enablers to optimal infant and young children’s oral care. Parents face complex decision-making challenges regarding their young children’s oral health care, particularly managing actual and perceived conflicts with their child. Knowledge and social and family norms influence their approach to managing these barriers.
Conclusions
The key influences enabling or preventing optimal infant oral care identified in this study lay the foundation for interventions to target these behaviours. To encourage a timely first dental visit, parents need consistent messaging from dental and other health professionals. To encourage twice daily toothbrushing, parents need more support in managing their child’s behaviour and competing priorities.
Validating the Korean Version of the Personal Financial Wellness Scale
Authors: Kim Yongseok 
Published: February 2025

Abstract

In South Korea, there are many situations that can threaten financial well-being, a factor that has been known to affect both mental and physical health. However, there has so far not been a standardized instrument that can measure an individual’s subjective financial well-being. This study aims to validate the Korean version of the Personal Financial Wellness (PFW) scale. Widely used in previous studies, the PFW scale is an instrument that measures an individual’s subjective financial well-being. The Korean version of the PFW scale is validated with 2,044 adults who participated in an online survey in South Korea. The evaluation of the psychometric properties of the Korean version of the PFW scale indicates that it is a reliable and valid instrument that exhibits a single-factor structure like the English version. The development of the Korean version of the PFW scale provides a valuable instrument for researchers and practitioners in South Korea where an instrument measuring an individual’s subjective financial well-being does not exist.
Potable water as a source of intermediate and borderline-resistant Aspergillus and Candida strains
Authors: Novak Babič Monika , Gunde-Cimerman Nina 
Published: February 2025

ABSTRACT
The World Health Organization calls to assess possible health risks from emerging fungi originating not only from hospitals but also from the natural environment. Fungal contamination in oligotrophic water systems represents a public health concern due to the potential for the emergence of antifungal-resistant strains. This study focused on the identification of Aspergillus spp. and Candida spp. isolated from different water sources and materials in contact with water. Isolated strains have been tested against nine antifungals to assess the prevalence of resistance in these strains. Only one strain of Aspergillus protuberus was resistant to amphotericin B. On the other hand, all Candida strains were intermediately resistant to anidulafungin and micafungin, 5.8% were borderline resistant to 5-flucytosine and fluconazole, and 3% to voriconazole. Candida parapsilosis sensu stricto isolated from water samples had statistically higher minimal inhibitory concentration (MIC) for anidulafungin than clinical strains and clinical strains had statistically higher MIC for itraconazole. Statistical analysis pointed out habitat to be significant for higher MIC in C. parapsilosis. Our findings show that borderline-resistant strains can be transferred by water; thus, potable water should be considered as a possible source of resistant strains in hospitals and healthcare units.
Drinking private well water: Groundwater quality and management of wells in southern Alabama
Authors: Millner Sidney , Malina Natalia , Rogers Stephanie R. , Henderson Emma , Ojeda Ann S. 
Published: February 2025

ABSTRACT

Private wells provide a source of household water for over 40 million people in the United States and an estimated 1 million people in the Coastal Lowlands Aquifer system along the Gulf of Mexico. Well water quality is dependent on the local geology and factors that contribute to anthropogenic contamination from the surface. Here, we evaluated groundwater quality and well management in southern Alabama, USA, to better understand factors that influence exposures through drinking water from private wells. The most common constituents that exceeded USEPA primary or secondary human health benchmarks were pH (92%), and total coliform (TC) (25%), followed by Fe (7%), Pb (6%), nitrate (1%), and As (1%). Most wells (68%) also displayed temporal changes in the number of exceedances, often showing positive for TC during one sampling campaign and negative in another, while the secondary standard for pH (6.5-8.5) was consistently not met. We also found that the common choices of water treatment did not protect against the most common water quality exceedances. Our results underscore the need to understand well water quality coupled with management practices when assessing potential exposures to the private well population through drinking water.
Epidemiological monitoring of sewage sludge and wastewater from an upflow anaerobic sludge blanket reactor using SARS-CoV-2 as a model
Authors: Wagner João Pedro Stepan , Rech Maria Fernanda , Prandi Bruno Aschidamini , Franco Ana Cláudia , Rigotto Caroline , Horn Fabiana 
Published: February 2025

ABSTRACT

This study explores the potential of sludge-based monitoring from an upflow anaerobic sludge blanket reactor for epidemiological surveillance, using SARS-CoV-2 as a model. We monitored SARS-CoV-2 copy numbers and mutations, and compared concentrations in sludge to concentrations in wastewater samples taken on the same days. From January to August 2021, 32 sludge samples were analyzed; 30 (93%) were positive for SARS-CoV-2, and copy numbers varied from 0.147 to 2.314 copies ×106/L. In wastewater samples collected on the same days, 31 (96%) were positive for SARS-CoV-2, and copy numbers ranged from 0.058 to 3.014 copies ×106/L. The concentration of SARS-CoV-2 in the sludge rose along with confirmed hospitalization cases in March, while wastewater SARS-CoV-2 concentrations rose 2 weeks earlier along with numbers of new confirmed cases. Mutations of variants of concern, Gamma and Delta, were identified in sludge samples in the same months that they became dominant in the corresponding regions. Our results indicate that, although monitoring of sewage sludge was not effective in anticipating infection numbers, it is a promising way to gain insight into the epidemiological situation in a city or region.
Colour discrimination in post-COVID-19 observers assessed by the Farnsworth-Munsell 100-Hue test
Authors: Griber Yulia A. , Paramei Galina V. 
Published: February 2025

Introduction. Post-COVID-19, various ophthalmological symptoms and visual impairments have been reported. We hypothesised that colour vision may be affected too. Methods. We assessed colour discrimination using the Farnsworth-Munsell 100 Hue test (FM-100) in individuals who have had COVID-19 (N = 77; 18–68 years). Results. Total error score (TES) indicated superior colour discrimination in 34 observers. The Vingrys–King-Smith C-index (severity) exceeded the normal cut-off measure in 44 observers. In participants (N = 35) with average TES, the Vingrys–King-Smith analysis revealed subtle colour deficiencies – either a mild tritan defect (‘blue’ or blue-yellow) or moderate defect with a diffuse error pattern. A minor sub-sample manifested poor discrimination (N = 6) or colour vision loss (N = 2), with a tritan or diffuse error pattern. √TES negatively correlated with the post-illness period. In partial error score (√PES), B–Y errors prevailed, regardless of the elapsed post-illness period. Discussion. Overall, the results indicate that about half of those who have recovered from COVID-19 revealed mild Type III acquired colour discrimination loss, characteristic of retinal disorders and vascular disease. Conceivably, coronavirus infection caused hypoperfusion (reduced vascular supply) at the retinal and/or post-retinal stages of the visual system and affected neural mechanisms of colour discrimination. The mild impairment appears to be reversible with a favourite prognosis.
Психологические индикаторы субъектности жизненной позиции
Authors: Фоминых Екатерина С. 
Published: February 2025

Introduction. The relevance of the issues of constructing a person's life path is conditioned by intensive modern transformations and the need to function in conditions of uncertainty, innovation, risks, multitasking and time deficit. The focus of the present study is the identification of psychological indicators of the subjectivity of life position in youth (chronotopic, metacognitive and reflexive). Methods. The diagnostic study was conducted with the participation of girls and young men studying undergraduate programmes at the Orenburg State Pedagogical University (n = 217, age of the subjects 19-24 years old). The psychodiagnostic complex consisted of standardised methods of diagnostics of life position, temporality, psychological boundaries, metacognitive processes and self-constructs: "Personal Life Position Questionnaire (PLQ)", "Temporal Modalities of Life Existence", "Psychological Boundary Diagnostic Methodology", "Analyticity-Holistic Scale", "Self-Efficacy Scale", "Tolerance-Intolerance to Uncertainty Questionnaire", "Related Self-Concept", "Collective and Independent Self-Concept". Results. The results of the conducted research fix the connection of subjectivity of the life position with the balance of modal evaluations, rationalisation of time periods of life realization, giving and taking in functions of psychological boundaries, tolerance to uncertainty, self-efficacy, independent self-concept. Discussion. Psychological indicators of the subjectivity of the position in youth are a holistic and authentic perception of the present, a meaningful attitude to time, its perception as a resource for achieving goals and realising life tasks. The balanced information-energy exchange between the external and internal spaces of the personality, provided by the intensity and vigour of the passing functions of psychological boundaries (taking in and giving away), is significant for the formation of the subjectivity of the life position. The importance of intrasubjective self-constructs in the formation of the subject's position in adolescence is proved. The analysis of significant links between the subjectivity of the position and metacognitive processes - self-efficacy, tolerance to uncertainty - is presented. In adolescence, the identified indicators reveal the potential of personality in the independent choice of goals and strategies for their achievement, building an authentic life line on the basis of an active, responsible and conscious attitude to their own lives, constructive creative self-realisation and self-development.
Imine Derivatives of Hydrochlorothiazide Bear Potential Diuretic Activity in in-vivo Experimental Model by Targeting Renal Outer Medullary Potassium Channel
Authors: Warda Hafiza Saliha , Sultana Kishwar , Ullah Irfan , Jan Afnan , Abdellattif Magda H. , Kashtoh Hamdy , Ur Rehman Najeeb , Khan Ajmal , Al-Harrasi Ahmed 
Published: February 2025

Introduction:
This study involves several recently synthesized Schiff base derivatives
of hydrochlorothiazide (3a-3e), characterized by advanced spectroscopic techniques including
Fourier transform infrared (FTIR), 1H-, 13C-NMR (Nuclear magnetic resonance),
and High-resolution electrospray ionization mass spectrometry (HR-ESI-MS).

Method:
Hydrochlorothiazide (HCTZ, 2 mM) was mixed with 2 mM aldehydes in ethanol
(20 mL) in the presence of glacial acetic acid, and the product was stirred with the help of a
magnetic stirrer under the specified conditions. Keeping in mind the importance of imine
derivatives against DPPH radical, the synthesized compounds were evaluated for antioxidant
activity (in-vitro) using DPPH assay and diuretic activity (in-vivo) by Lipschitz
method. All derivatives demonstrated notable antioxidant activity, exhibiting 52-86% inhibition.

Result:
The compounds 3a-3e also displayed remarkable diuretic effects in an in-vivo model
at a dose of 50 mg/kg. The computational studies of all the newly synthesized compounds
were interpreted for the diuretic activity at the molecular level against human renal outer medullary
potassium channel (ROMK1, PDB: ID) protein present on the nephron involved in
diuresis indicating all compounds showed significant interactions at all receptors.

Conclusion:
As the derivatives were more active than drugs in some regards; therefore, these
derivatives will be beneficial and open new windows for research and additional pharmacological
screening could be planned as part of the project's extension.
Lipid Biochemistry and its Role in Human Diseases
Authors: Nehaoua Amine , Gasmi Amin , Benahmed Asma Gasmi , Noor Sadaf , Bjørklund Geir 
Published: February 2025

Abstract:
Lipids play a variety of roles in living systems. They are a source of extremely
high energy and a part of almost all signaling and biological processes. Despite the liver
being the hub of lipid metabolism, lipid metabolism occurs across the human body.
Any perturbation in the lipid metabolism or lipid storage systems can lead to diseases or
disorders that can hamper the normal functioning of the human body. Lipids have been
explored for their role in cancers. The intake of saturated fatty acids has been found to increase
the metastasis and growth of cancerous cells. The role of lipids has also been
studied in brain diseases. In Tay-Sachs disease, the inability to metabolize GM2 ganglioside
alters normal nerve cell functioning. Similarly, lipids also play critical roles in
Parkinson's and Alzheimer’s disease. Moreover, atherosclerosis is a leading cause of cardiovascular
diseases and brain stroke. Dyslipidemia or excess fatty acids is a leading
cause of non-alcoholic fatty liver disease, insulin resistance, and diabetes mellitus. Dyslipidemia
also leads to jaundice, which, in turn, can seriously damage the kidneys. This
review focuses on the various human diseases occurring because of lipid metabolism.
The Multifaceted Roles of Thrombomodulin: Anti-coagulation, Anti-inflammation, and Anti-tumor Potential
Authors: Yang Jiaying , Gu Yongxing , Wang Tianyin , Zhang Jingjing , Zhang Xiong , Yu Feng 
Published: February 2025

Abstract:
Thrombomodulin (TM) is a single-chain transmembrane glycoprotein with anticoagulant effects.
TM has two forms: membrane type existing on the cell surface and blood type free in plasma and urine. TM
functions as an anticoagulant cofactor for thrombin activation of protein C on the surface of vascular endothelial
cells. Due to the excellent anti-coagulant effects in modulating the coagulation and fibrinolytic system, the
recombinant human soluble TM (rhsTM) has been used for the treatment of disseminated intravascular coagulation
(DIC). In addition to anti-coagulation, many studies have shown that TM can also exert anti-inflammatory
and anti-tumor effects. TM has a lectin-like domain at its N-terminus that has been shown to exhibit direct
anti-inflammatory functions. At the same time, due to its special structure, thrombomodulin plays an important
role in vascular-related mechanistic diseases by participating in the regulation of inflammatory pathways,
complement, HMGB1, etc. In this article, changes in TM expression in the body after injury, composition of
TM structural domains, anticoagulant, anti-inflammatory, and antitumor effects, and related mechanisms of
TM were systematically reviewed, to provide a theoretical basis and reference for the potential clinical implications
of TM in treating various diseases.
A Review Unveiling the Ferroptosis-Regulated Cell Signalling Pathways in Breast Cancer to Elucidate Potent Targets for Cancer Management
Authors: Pandey Pratibha , Pandey Shivam , Ramniwas Seema , Ballal Suhas , Kumar Sanjay , Bhat Mahakshit , Sharma Shilpa , Kumar M. Ravi , Khan Fahad 
Published: February 2025

Abstract:
Recent research suggests that targeting ferroptosis exhibits promise as a potent treatment approach
for breast carcinoma. Specific subtypes of tumor cells exhibit heightened vulnerability to ferroptosis-inducing
chemicals, which selectively trigger tumor stem cells' demise, enhance tumor cells' sensitivity to chemotherapeutic
drugs, and eliminate cancerous cells. Ferroptosis plays a dual role in breast cancer progression, emerging
as both a stimulating and inhibitory component. Ferroptosis is effective in treating cancer cells (mesenchymal
breast), identified by their ability to undergo Epithelial-mesenchymal Transition (EMT) and their resistance
to conventional therapies. Pharmaceutical drugs that hinder the activity of enzymes known as kinases,
which are involved in the AKT/mTOR/PI3K signaling pathway, have shown significant potential in the treatment
of breast carcinoma. This review investigates the molecular mechanisms of different signaling pathways
implicated in ferroptosis in breast carcinoma, with specific emphasis on metastasis, invasion, and proliferation.
Our study contributes to understanding a potentially important target that could be used in developing
therapeutic strategies for breast cancer treatment.
Hsa_circ_0030568 Promotes the Proliferation and Migration of Prostate
Cancer Cells via the miR-141-3p-BRD4 Axis
Authors: Sun Xiaoxiao , Xiang Congming , Wu Yuwei , Ge Dongsheng , Sun Chengwen 
Published: February 2025

Introduction:
Numerous studies have indicated that circular RNAs (circRNAs) play
an important role in diverse cancers. However, the specific molecular mechanism of circRNAs
in prostate cancer (PCa) remains largely unclear.

Method:
In this study, we performed western blotting, real-time quantitative polymerase chain
reaction, CCK-8 assay, cell scratch wound assay, and RNA immunoprecipitation assay.

Results:
These results showed that the expression of hsa_circ_0030568 was remarkably increased in PCa cells by using quantitative real-time PCR combined with dataset analysis.
Mechanistically, circ_0030568 could physically bind to miR-141-3p and elevate the expression of
BRD4 via sponging miR-141-3p in PCa cells, then accelerating the progression of PCa. Upregulation of hsa_circ_0030568 induces tumor progression through the miR-141-3p-BRD4 axis.

Conclusion:
Taken together, these findings suggested that hsa_circ_0030568 may act as a potential biomarker of PCa and a promising target for PCa treatment.