bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between active commuting and low-grade inflammation: a population-based cross-sectional study
Authors: Allaouat Sara , Halonen Jaana I , Jussila Juuso J , Tiittanen Pekka , Ervasti Jenni , Ngandu Tiia , Mikkonen Santtu , Yli-Tuomi Tarja , Jousilahti Pekka , Lanki Timo 
Published: December 2023

Abstract

Background
Prior studies suggest that physical activity lowers circulating C-reactive protein (CRP) levels. However, little is known about the association between regular active commuting, i.e. walking or cycling to work, and CRP concentrations. This study examines whether active commuting is associated with lower CRP.

Methods
We conducted a cross-sectional study using population-based FINRISK data from 1997, 2002, 2007 and 2012. Participants were working adults living in Finland (n = 6208; mean age = 44 years; 53.6% women). We used linear and additive models adjusted for potential confounders to analyze whether daily active commuting, defined as the time spent walking or cycling to work, was associated with lower high-sensitivity (hs-) CRP serum concentrations compared with passive commuting.

Results
We observed that daily active commuting for 45 min or more (vs. none) was associated with lower hs-CRP [% mean difference in the main model: −16.8%; 95% confidence interval (CI) −25.6% to −7.0%), and results were robust to adjustment for leisure-time and occupational physical activity, as well as diet. Similarly, active commuting for 15–29 min daily was associated with lower hs-CRP in the main model (−7.4; 95% CI −14.1 to −0.2), but the association attenuated to null after further adjustments. In subgroup analyses, associations were only observed for women.

Conclusions
Active commuting for at least 45 min a day was associated with lower levels of low-grade inflammation. Promoting active modes of transport may lead not only to reduced emissions from motorized traffic but also to population-level health benefits.
Loss and recovery of ecological diversity associated with evolutionary rescue in abruptly and gradually deteriorating environments
Authors: Zhou Dong-Hao , Zhang Quan-Guo 
Published: December 2023

Abstract
Populations may survive environmental deterioration by evolutionary adaptation. However, such evolutionary rescue events may be associated with ecological costs, such as reduction in growth performance and loss of ecologically important genetic diversity. Those negative ecological consequences may be mitigated by additional adaptive evolution. Both the ecological costs and the opportunities for additional evolution are contingent on the severity of environmental deterioration. Here, we hypothesize that populations evolutionarily rescued from faster, relative to slow, environmental deterioration suffer more severe long-term fitness decline and diversity loss. An experiment with the model adaptive radiation of bacterium Pseudomonas fluorescens exposed to abruptly or gradually increased antibiotic stress supported our hypothesis. The effect of additional adaptive evolution in recovering population size and ecological diversity was far from perfect. Cautions are therefore needed in predicting the role of rapid evolution for mitigating the impacts of environmental changes, in particular very fast environmental deterioration. We also found that bacterial populations rescued from gradually increased antibiotic stress evolved higher levels of antibiotic resistance, lending more support to aggressive chemotherapy in pathogen control.
Pathogenic characteristics of the Vibrio parahaemolyticus which caused a gastroenteritis outbreak event in Huzhou
Authors: Wu Xiaofang , Zhu Yongying , Yan Wei , Zhang Peng , Chen Liping 
Published: December 2023

Abstract
The pathogenic characteristics of V. parahaemolyticus isolated from a gastroenteritis outbreak event in Deqing County of Huzhou City in 2022 were analyzed. Pathogen detection was performed on 30 anal swabs (26 patients, 1 chef and 3 waiters). The isolates of V. parahaemolyticus were analyzed by serum typing, pulsed field gel electrophoresis (PFGE) molecular typing, multiplex fluorescent PCR detection of tdh/trh virulence gene and drug sensitivity test. 15 patients were positive for V. parahaemolyticus, 1 patient was positive for V. parahaemolyticus and Enteroaggregative E. coli (EAEC), 1 patient was positive for EAEC, and the chef was positive for EAEC. The serotype test results of the 16 V. parahaemolyticus were 14 O4:KUT and 2 O10:K4. All samples were negative for other tested bacteria. All V. parahaemolyticus strains were positive for tdh genes and negative for trh gene. The 16 isolates were 100% resistant to ampicillin (AMP), and sensitive to the other12 antibiotics. From the results of serotype and PFGE, the V. parahaemolyticus strains with two serotypes are clustered into two branches according to their serotypes. The three EAEC strains were non-homologous. In conclusion, we detected V. parahaemolyticus and EAEC from an outbreak of gastroenteritis. And V. parahaemolyticus with two serotypes may be the cause of this event, according to the traceability results.
Fabrication of telechelic DNA-bridged food emulsion gel as edible ink for 3D printing
Authors: Zhang Ruyuan , Huang Hao , Ai Rong , Li Dong , Xu Yanqun , Jin Weiwei , Luo Zisheng 
Published: December 2023

Abstract

Objectives
Interdroplet interactions affect the properties and textures of emulsions. In this study, we creatively introduced telechelic DNA to link oil droplets directly at molecular scale to further improve the viscoelastic properties of emulsions.

Materials and Methods
A stable emulsion with 70% oil fraction was prepared by adding 40 mg/mL of whey protein isolate and peach gum polysaccharide complex (WPI–PGP complex). The addition of telechelic DNA (0.125–0.375 μmol/L) made the emulsion change from a cream-like state to a self-supporting gel.

Results
Rheological experiments confirmed that the telechelic DNA could improve the yield stress, storage modulus/loss modulus (Gʹ/Gʹʹ) and apparent viscosity of the emulsion gel in a concentration-dependent manner (0–0.375 μmol/L). Inverted fluorescence images clearly showed the interdroplet network of the emulsion gel linked by telechelic DNA. For 3D printing, the emulsion gel with a higher concentration (0.375 μmol/L) of telechelic DNA displayed better surface quality and dimensional resolution, indicating improved printability.

Conclusion
This study successfully designed a novel telechelic DNA-bridged emulsion gel, which showed great potential as edible ink for 3D printing.
Assembly and annotation of 2 high-quality columbid reference genomes from sequencing of a Columba livia × Columba guinea F1 hybrid
Authors: Maclary Emily T , Holt Carson , Concepcion Gregory T , Sović Ivan , Vickrey Anna I , Yandell Mark , Kronenberg Zev , Shapiro Michael D 
Published: December 2023

Abstract
Pigeons and doves (family Columbidae) are one of the most diverse extant avian lineages, and many species have served as key models for evolutionary genomics, developmental biology, physiology, and behavioral studies. Building genomic resources for columbids is essential to further many of these studies. Here, we present high-quality genome assemblies and annotations for 2 columbid species, Columba livia and Columba guinea. We simultaneously assembled C. livia and C. guinea genomes from long-read sequencing of a single F1 hybrid individual. The new C. livia genome assembly (Cliv_3) shows improved completeness and contiguity relative to Cliv_2.1, with an annotation incorporating long-read IsoSeq data for more accurate gene models. Intensive selective breeding of C. livia has given rise to hundreds of breeds with diverse morphological and behavioral characteristics, and Cliv_3 offers improved tools for mapping the genomic architecture of interesting traits. The C. guinea genome assembly is the first for this species and is a new resource for avian comparative genomics. Together, these assemblies and annotations provide improved resources for functional studies of columbids and avian comparative genomics in general.
Bradysia (Sciara) coprophila larvae up-regulate DNA repair pathways and down-regulate developmental regulators in response to ionizing radiation
Authors: Urban John M , Bateman Jack R , Garza Kodie R , Borden Julia , Jain Jaison , Brown Alexia , Thach Bethany J , Bliss Jacob E , Gerbi Susan A 
Published: December 2023

Abstract
The level of resistance to radiation and the developmental and molecular responses can vary between species, and even between developmental stages of one species. For flies (order: Diptera), prior studies concluded that the fungus gnat Bradysia (Sciara) coprophila (sub-order: Nematocera) is more resistant to irradiation-induced mutations that cause visible phenotypes than the fruit fly Drosophila melanogaster (sub-order: Brachycera). Therefore, we characterized the effects of and level of resistance to ionizing radiation on B. coprophila throughout its life cycle. Our data show that B. coprophila embryos are highly sensitive to even low doses of gamma-irradiation, whereas late-stage larvae can tolerate up to 80 Gy (compared to 40 Gy for D. melanogaster) and still retain their ability to develop to adulthood, though with a developmental delay. To survey the genes involved in the early transcriptional response to irradiation of B. coprophila larvae, we compared larval RNA-seq profiles with and without radiation treatment. The up-regulated genes were enriched for DNA damage response genes, including those involved in DNA repair, cell cycle arrest, and apoptosis, whereas the down-regulated genes were enriched for developmental regulators, consistent with the developmental delay of irradiated larvae. Interestingly, members of the PARP and AGO families were highly up-regulated in the B. coprophila radiation response. We compared the transcriptome responses in B. coprophila to the transcriptome responses in D. melanogaster from 3 previous studies: whereas pathway responses are highly conserved, specific gene responses are less so. Our study lays the groundwork for future work on the radiation responses in Diptera.
Fusion of altimetry-derived model and ship-borne data in preparation of high-resolution marine gravity determination
Authors: Chen Xu , Kong Xiangxue , Zhou Runsheng , Zhang Shengjun 
Published: December 2023

SUMMARY
Satellite altimetry provides major data sources for marine gravity recovery, and typical altimetry derived models, for example, DTU21 and SS V32.1, were usually released with 1 arcmin × 1 arcmin gridding interval. Their true resolution is much lower than the nominal ∼2 km level. By contrast, the in situ ship-borne measurements are considered to have better short-wavelength resolution. In this paper, we aim to propose a new method to fully utilize satellite altimetry data and ship-borne measurements, namely the frequency-domain fusion method, and give certain analysis of new method along with two spatial-domain fusion methods. Comprehensive analysis is focus on four aspects: gravity signals in fusion images, numerical verifications, power spectra, as well as coherence analysis. Initial evaluation indicates that, first, the frequency-domain fusion method has advantage in flexibility, since it can autonomously select dominant bands to fuse different data sets. Secondly, the new method retains medium-long wavelength signals from altimetry-derived model and effectively incorporate dominant short-wavelength signals of in-situ measurements, while the spatial-domain methods are essentially full-wavelength fusion and inevitably diminish the role of satellite altimetry. To some extent, the new method maximize the positive contribution of satellite altimetry measurements and efficiently exploit the benefits of ship-borne data. Finally, verification experiments were similarly designed in three regions with different amount and ratio of ship-borne data to thoroughly evaluate various methods. In two regions with gridded and dense along-cruise ship-borne data, the average accuracy of this frequency-domain fusion results is improved by 0.346 and 0.613 mGal, respectively. In a region with sparse ship-borne data, we still recommend using spatial-domain fusion methods since the new method is unable to align ship-borne data with model grid. It is concluded from the above analysis that the new method effectively incorporates the short-wavelength signals from ship-borne data into the altimetry-derived gravity field model, and it is significant that the new method maximizes the application of advantageous bands from different data sources.
Phosphate deficiency induced by infection promotes synthesis of anthracnose-resistant anthocyanin-3-O-galactoside phytoalexins in the Camellia sinensis plant
Authors: Li Tongtong , Wang Shenrong , Shi Dandan , Fang Wen , Jiang Ting , Zhang Lixin , Liu Yajun , Gao Liping , Xia Tao 
Published: December 2023

Abstract
Tea (Camellia sinensis) is a well-known beverage crop rich in polyphenols with health benefits for humans. Understanding how tea polyphenols participate in plant resistance is beneficial to breeding resistant varieties and uncovering the resistance mechanisms. Here, we report that a Colletotrichum infection-induced ‘pink ring’ symptom appeared outside the lesion, which is highly likely to occur in resistant cultivars. By identifying morphological feature-specific metabolites in the pink ring and their association with disease resistance, and analysis of the association between metabolite and gene expression, the study revealed that the accumulation of anthocyanin-3-O-galactosides, red phytotoxin compounds resistant to anthracnose, plays a pivotal role in the hypersensitive response surrounding infection sites in tea plants. The results of genetic manipulation showed that the expression of CsF3Ha, CsANSa, CsUGT78A15, CsUGT75L43, and CsMYB113, which are involved in anthocyanin biosynthesis, is positively correlated with anthracnose-resistance and the formation of the pink ring. Further phosphorus quantification and fertilization experiments confirmed that phosphate deficiency caused by anthracnose is involved in the occurrence of the pink ring. Genetic manipulation studies indicated that altering the expression levels of Pi transporter proteins (CsPHT2-1, CsPHT4;4) and phosphate deprivation response transcription factors (CsWRKY75-1, CsWRKY75-2, CsMYB62-1) enhances resistance to anthracnose and the formation of the pink ring symptom in tea plants. This article provides the first evidence that anthocyanin-3-O-galactosides are the anthracnose-resistant phytoalexins among various polyphenols in tea plants, and this presents an approach for identifying resistance genes in tea plants, where genetic transformation is challenging.
The Impact of Neighborhood-Level Factors on Perceived Quality of Hospice Service Satisfaction: A Geographic Information System Study
Authors: Sualp Kenan , Beltran Susanny , Chen Guochao 
Published: December 2023

Abstract
Racial/ethnic minorities are underrepresented in hospice care in the United States, despite this being the standard of care for terminally ill individuals. Research indicates that location of services (e.g., rural vs. urban) plays a role in the differences in quality and quantity of services observed. However, the influences of neighborhood structural characteristics on quality of hospice services have not been explored. The purpose of this study was to explore the relationship between hospice neighborhood characteristics and caregiver-rated satisfaction with services in Florida. Data were derived from the Consumer Assessment of Healthcare Providers and Systems Hospice Survey and American Community Survey; ArcGIS was used to generate heat maps to provide visual representations. Findings showed that higher neighborhood immigrant density predicted lower family satisfaction with timely care, communication, spiritual support, and likelihood to recommend the agency services. The U.S. population is projected to continue to grow in its diversity, and thus understanding neighborhood characteristics associated with racial/ethnic minorities’ perception of care quality are important for shaping care improvements.
How smart is a ‘smart factory’?: an organizational view
Authors: Chung Sunghoon , Kim Minho 
Published: December 2023

Abstract
This paper investigates the game-changing paradigm in modern manufacturing, known as the “smart factory,” from an organizational perspective. Factories get smarter—or smartized—by becoming more data-intensive and more tightly integrating operations with management. We design a unique survey to measure this intangible organizational capital accumulation process for 939 Korean manufacturing plants. Observing heterogeneous levels of smartization across factories, we explore their relationship with three performance dimensions: productivity, cost efficiency, and product variety. The drivers of smartization are also identified. Our analysis uncovers a strong association between smartization and heightened productivity, with varying interactions with other performance metrics based on the specific manufacturing process (e.g., lower defect rate only in assembly line process). Importantly, we find that investments in digital technologies drive smartization only when complemented by structured incentive management and proper chief executive officer (CEO) leadership.
El proceso constituyente español de 1873: origen y fracaso de la República FederalThe Spanish constituent process of 1873: origin and failure of the Federal Republic
Authors: Marzal Yetano Elia 
Published: December 2023

Abstract
The Spanish constitutional project of 1873 was intended to lay the foundations of the First Spanish Republic, whose proclamation took place in the context of the abdication of King Amadeo I to the throne. In this article I approach the analysis of the constituent process from a double perspective. On the one hand, that of the methodological rule of the possibility principle, which requires the project having a minimum probability of success in order to contribute to the theory of constituent processes. On the other hand, that of theory building process tracing, which allows us to causally reconstruct the failure and identify the intervening factors. I argue that, although the particular seriousness of the political crisis that existed in Spain at the beginning of the constituent process did not necessarily condemn it to failure, as the process progressed, the worsening of these circumstances, together with the novel but also divisive nature of the project, ended up dynamiting the initial consensus.
El fracaso del proceso constituyente en KeniaThe failure of the constituent process in Kenya
Authors: Arias Guedón Sonsoles 
Published: December 2023

Abstract
Kenya began its democratic era after its declaration of independence and the adoption of its first constitution in 1963. However, this supreme norm of the country would undergo countless reforms by the political elites from the very beginning, mostly to satisfy the partisan demands of the political leaders at the moment. These reforms succeeded in transforming the initially democratic model into an authoritarian, one-party system. After two decades of corruption and abuse of power, the political changes in the country and the wave of democratization after the fall of the Berlin Wall fueled the popular clamour for constitutional change. This article analyses the development of the constitution-making process, which began with the first constitutional amendments introduced to the constitution of the independence, as well as the main factors that led to the rejection of the new draft constitution in the ratification referendum held on 21 November 2005. A key element contributing to the failure of the whole process is the strong influence of political forces in the process and the partisan use of all of them to achieve their political advantages.
Longitudinal clustering of Life’s Essential 8 health metrics: application of a novel unsupervised learning method in the CARDIA study
Authors: Graffy Peter , Zimmerman Lindsay , Luo Yuan , Yu Jingzhi , Choi Yuni , Zmora Rachel , Lloyd-Jones Donald , Allen Norrina Bai 
Published: December 2023

Abstract

Objective
Changes in cardiovascular health (CVH) during the life course are associated with future cardiovascular disease (CVD). Longitudinal clustering analysis using subgraph augmented non-negative matrix factorization (SANMF) could create phenotypic risk profiles of clustered CVH metrics.

Materials and methods
Life’s Essential 8 (LE8) variables, demographics, and CVD events were queried over 15 years in 5060 CARDIA participants with 18 years of subsequent follow-up. LE8 subgraphs were mined and a SANMF algorithm was applied to cluster frequently occurring subgraphs. K-fold cross-validation and diagnostics were performed to determine cluster assignment. Cox proportional hazard models were fit for future CV event risk and logistic regression was performed for cluster phenotyping.

Results
The cohort (54.6% female, 48.7% White) produced 3 clusters of CVH metrics: Healthy & Late Obesity (HLO) (29.0%), Healthy & Intermediate Sleep (HIS) (43.2%), and Unhealthy (27.8%). HLO had 5 ideal LE8 metrics between ages 18 and 39 years, until BMI increased at 40. HIS had 7 ideal LE8 metrics, except sleep. Unhealthy had poor levels of sleep, smoking, and diet but ideal glucose. Race and employment were significantly different by cluster (P &lt; .001) but not sex (P = .734). For 301 incident CV events, multivariable hazard ratios (HRs) for HIS and Unhealthy were 0.73 (0.53-1.00, P = .052) and 2.00 (1.50-2.68, P &lt; .001), respectively versus HLO. A 15-year event survival was 97.0% (HIS), 96.3% (HLO), and 90.4% (Unhealthy, P &lt; .001).

Discussion and conclusion
SANMF of LE8 metrics identified 3 unique clusters of CVH behavior patterns. Clustering of longitudinal LE8 variables via SANMF is a robust tool for phenotypic risk assessment for future adverse cardiovascular events.
Validation of the FSTestTM Aerobic Count Plates Method for Enumeration of Aerobic Bacteria in a Variety of Matrixes and Stainless Steel Environmental Surface: AOAC Performance Tested MethodSM 112301
Authors: Liu Yun , Fan Jun , Sun Xia , Zhou Zican 
Published: December 2023

Abstract

Background
The FSTestTM Aerobic Count (AC) Plates are ready-to-use culture media containing nutrients, a cold-water-soluble gelling agent, and a chromogenic indicator.

Objective
The objective of this study was to validate the FSTest AC plate method for AOAC INTERNATIONAL Performance Tested MethodsSM (PTM) certification for a variety of foods and stainless steel.

Methods
The performance of the FSTest AC plates were compared to the appropriate reference method, for the detection of total aerobic bacterial in a variety of foods matrixes (raw ground beef, raw ground pork, cooked ham, raw chicken breast, raw shrimp, frozen tuna, shredded bagged lettuce, cherry tomato, pasteurized liquid milk, nonfat milk powder) and on stainless steel surfaces. Robustness, consistency, and stability studies of the FSTest AC plate were also conducted.

Results
The results of the matrix study showed the standard deviation of repeatability (sr) was similar in both the FSTest AC plate method and the reference method. The 90% confidence interval of the difference between means between the two methods was found to fall within −0.5 to 0.5 log10 for all matrixes at all levels in the method developer and independent laboratory studies. The data in the report also support that the FSTest AC plate method is robust, manufactured in a consistent manner, and can be stable for 18 months at 4–10°C.

Conclusions
The FSTest AC method is validated to be equivalent to the appropriate reference methods for the enumeration of aerobic bacteria in a variety of food matrixes and on stainless steel surfaces at 36 ± 1°C, and 32 ± 1°C (for dairy matrixes) in 24 ± 1 h.

Highlights
The FSTest AC plate method offers the advantage of saving labor, space, and time, as results are available within 24 h for all tested matrixes.