bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glycoconjugate Binding of Gastric and Enterohepatic
Helicobacter
spp
Authors: Hynes Sean O. , Teneberg Susann , Roche Niamh , Wadström Torkel 
Published: April 2003

ABSTRACT

Helicobacter pylori
is able to utilize several lectin-like, protein-carbohydrate interactions for binding to mucins, cell surfaces, and extracellular matrix proteins. As determined by hemagglutination assays and binding of radiolabeled bacteria to glycosphingolipids on thin-layer chromatograms, strains of gastric helicobacters and enterohepatic helicobacters, including
Helicobacter canis, Helicobacter hepaticus
, and
Helicobacter bilis
, also demonstrated evidence for the presence of lectin-hemagglutinin adhesins. In addition, in
H. hepaticus
and
H. bilis
, binding may be sialic acid dependent. The presence or absence and differences in the levels of activity of lectin adhesins may reflect the species' ecological niche.
Microenvironment‐induced restoration of cohesive growth associated with focal activation of P‐cadherin expression in lobular breast carcinoma metastatic to the colon
Authors: Gronewold Malte , Grote Isabel , Bartels Stephan , Christgen Henriette , Kandt Leonie D , Brito Maria Jose , Cserni Gàbor , Daemmrich Maximilian E , Fogt Franz , Helmke Burkhard M , ter Hoeve Natalie , Lang‐Schwarz Corinna , Vieth Michael , Wellmann Axel , Kuehnle Elna , Kulik Ulf , Riedel Gesa , Reineke‐Plaass Tanja , Lehmann Ulrich , Koorman Thijs , Derksen Patrick WB , Kreipe Hans , Christgen Matthias 
Published: January 2024

AbstractInvasive lobular carcinoma (ILC) is a special breast cancer type characterized by noncohesive growth and E‐cadherin loss. Focal activation of P‐cadherin expression in tumor cells that are deficient for E‐cadherin occurs in a subset of ILCs. Switching from an E‐cadherin deficient to P‐cadherin proficient status (EPS) partially restores cell–cell adhesion leading to the formation of cohesive tubular elements. It is unknown what conditions control EPS. Here, we report on EPS in ILC metastases in the large bowel. We reviewed endoscopic colon biopsies and colectomy specimens from a 52‐year‐old female (index patient) and of 18 additional patients (reference series) diagnosed with metastatic ILC in the colon. EPS was assessed by immunohistochemistry for E‐cadherin and P‐cadherin. CDH1/E‐cadherin mutations were determined by next‐generation sequencing. The index patient's colectomy showed transmural metastatic ILC harboring a CDH1/E‐cadherin p.Q610* mutation. ILC cells displayed different growth patterns in different anatomic layers of the colon wall. In the tunica muscularis propria and the tela submucosa, ILC cells featured noncohesive growth and were E‐cadherin‐negative and P‐cadherin‐negative. However, ILC cells invading the mucosa formed cohesive tubular elements in the intercryptal stroma of the lamina propria mucosae. Inter‐cryptal ILC cells switched to a P‐cadherin‐positive phenotype in this microenvironmental niche. In the reference series, colon mucosa infiltration was evident in 13 of 18 patients, one of which showed intercryptal EPS and conversion to cohesive growth as described in the index patient. The large bowel is a common metastatic site in ILC. In endoscopic colon biopsies, the typical noncohesive growth of ILC may be concealed by microenvironment‐induced EPS and conversion to cohesive growth.
Total scalp irradiation: A study comparing multiple types of bolus and VMAT optimization techniques
Authors: Davis Tanisha M. , Luca Kirk , Sudmeier Lisa J. , Buchwald Zachary S. , Khan Mohammad K. , Yang Xiaofeng , Schreibmann Eduard , Zhang Jiahan , Roper Justin 
Published: January 2024

AbstractPurposeTo investigate bolus design and VMAT optimization settings for total scalp irradiation.MethodsThree silicone bolus designs (flat, hat, and custom) from .decimal were evaluated for adherence to five anthropomorphic head phantoms. Flat bolus was cut from a silicone sheet. Generic hat bolus resembles an elongated swim cap while custom bolus is manufactured by injecting silicone into a 3D printed mold. Bolus placement time was recorded. Air gaps between bolus and scalp were quantified on CT images. The dosimetric effect of air gaps on target coverage was evaluated in a treatment planning study where the scalp was planned to 60 Gy in 30 fractions. A noncoplanar VMAT technique based on gEUD penalties was investigated that explored the full range of gEUD alpha values to determine which settings achieve sufficient target coverage while minimizing brain dose. ANOVA and the t‐test were used to evaluate statistically significant differences (threshold = 0.05).ResultsThe flat bolus took 32 ± 5.9 min to construct and place, which was significantly longer (p < 0.001) compared with 0.67 ± 0.2 min for the generic hat bolus or 0.53 ± 0.10 min for the custom bolus. The air gap volumes were 38 ± 9.3 cc, 32 ± 14 cc, and 17 ± 7.0 cc for the flat, hat, and custom boluses, respectively. While the air gap differences between the flat and custom boluses were significant (p = 0.011), there were no significant dosimetric differences in PTV coverage at V57Gy or V60Gy. In the VMAT optimization study, a gEUD alpha of 2 was found to minimize the mean brain dose.ConclusionsTwo challenging aspects of total scalp irradiation were investigated: bolus design and plan optimization. Results from this study show opportunities to shorten bolus fabrication time during simulation and create high quality treatment plans using a straightforward VMAT template with simple optimization settings.
Hydrogel Alginate Considerations for Improved 3D Matrix Stability and Cell Graft Viability and Function in Studying Type 1 Diabetes In Vitro
Authors: Quiroz Victor M. , Wang Yuanjia , Rakoski Amanda I. , Kasinathan Devi , Neshat Sarah Y. , Hollister‐Lock Jennifer , Doloff Joshua C. 
Published: January 2024

AbstractBiomedical devices such as islet‐encapsulating systems are used for treatment of type 1 diabetes (T1D). Despite recent strides in preventing biomaterial fibrosis, challenges remain for biomaterial scaffolds due to limitations on cells contained within. The study demonstrates that proliferation and function of insulinoma (INS‐1) cells as well as pancreatic rat islets may be improved in alginate hydrogels with optimized gel%, crosslinking, and stiffness. Quantitative polymerase chain reaction (qPCR)‐based graft phenotyping of encapsulated INS‐1 cells and pancreatic islets identified a hydrogel stiffness range between 600 and 1000 Pa that improved insulin Ins and Pdx1 gene expression as well as glucose‐sensitive insulin‐secretion. Barium chloride (BaCl2) crosslinking time is also optimized due to toxicity of extended exposure. Despite possible benefits to cell viability, calcium chloride (CaCl2)‐crosslinked hydrogels exhibited a sharp storage modulus loss in vitro. Despite improved stability, BaCl2‐crosslinked hydrogels also exhibited stiffness losses over the same timeframe. It is believed that this is due to ion exchange with other species in culture media, as hydrogels incubated in dIH2O exhibited significantly improved stability. To maintain cell viability and function while increasing 3D matrix stability, a range of useful media:dIH2O dilution ratios for use are identified. Such findings have importance to carry out characterization and optimization of cell microphysiological systems with high fidelity in vitro.
Trifluoromethyl‐Substituted Perylene Diimides for Rapid and Sensitive Film‐Based Fluorescence Detection of Methamphetamine and Cocaine Hydrochloride
Authors: Chen Ming , Chu Ronan , Babazadeh Mohammad , Kistemaker Jos C. M. , Burn Paul L. , Gentle Ian R. , Shaw Paul E. 
Published: January 2024

AbstractFilm‐based fluorescence sensors are attractive for rapid and on‐site detection of illicit drugs, due to their potential for portability, high sensitivity and reusability. Three new bay‐substituted perylene diimide derivatives are synthesized , P1‐CH3 (1‐methyl), P1‐CF3 (1‐trifluoromethyl), and P1‐(CF3)2 (1,7‐di‐trifluoromethyl) that can be solution processed into thin films capable of detecting methamphetamine hydrochloride (MA•HCl) and cocaine•HCl via a luminescence quenching mechanism. The ionization potentials of the materials are sufficiently high to enable photoinduced hole transfer to occur, which led to the luminescence quenching. The trifluoromethyl‐substituted PDIs exhibit superior sensitivity and reversibility of the luminescence quenching process. In particular, P1‐(CF3)2 shows a limit of detection to MA•HCl of ≈100 ng after a 15‐second exposure, and its luminescence can be fully recovered either through mild heating or being placed in an air flow. Furthermore, by using the luminescence quenching and recovery responses of four PDI sensing materials it is possible to differentiate MA•HCl, cocaine•HCl, from aspirin, caffeine, sugar, and (sebum) skin oil in around a minute.
High Energy Density Solid‐State Lithium Metal Batteries Enabled by In Situ Polymerized Integrated Ultrathin Solid Electrolyte/Cathode
Authors: Hu Jiang‐Kui , Gao Yu‐Chen , Yang Shi‐Jie , Wang Xi‐Long , Chen Xiang , Liao Yu‐Long , Li Shuai , Liu Jia , Yuan Hong , Huang Jia‐Qi 
Published: January 2024

AbstractSolid‐state batteries (SSBs) are regarded as the most promising next‐generation energy storage devices due to their potential to achieve higher safety performance and energy density. However, the troubles in the preparation of ultrathin solid‐state electrolytes (SEs) as well as the resultant compromise in mechanical strength greatly limit the safety application of SSBs. Herein, a novel in situ polymerized integrated ultrathin SE/cathode design is developed. The ultrathin ceramic layer supported on the cathode serves not only as a rigid scaffold to prevent direct contact between the cathode and anode but also as active inorganic fillers to enhance the mechanical properties of in situ polymerized SE film. The unique Li‐ion coordination environments as well as the Li hopping mechanism profoundly promote fast ion transport in composite SEs. The in situ polymerized SEs simultaneously achieve the balance in ultrathin thickness (10 µm), fast ion transport (0.65 mS cm−1), superior Young's modulus (66.8 GPa), and excellent interface contact. The pouch cells with practical Li||LiNi0.8Co0.1Mn0.1O2 configuration achieve an ultrahigh volumetric energy density of 1018 Wh L−1 and safety performance. The in situ polymerized integrated ultrathin SE/cathode design exhibits great promise for the practical application of SSBs with high energy density and safety performance.
Wearable and Regenerable Electrochemical Fabric Sensing System Based on Molecularly Imprinted Polymers for Real‐Time Stress Management
Authors: Hu Xiaokang , Chen Yangyang , Wang Xin , Jia Kangkang , Zhang Huanlei , Wang Yuxin , Chu Hongwei , Zhong Xiaohua , Lin Meng , Chen Peining , Qiu Longbin , Peng Huisheng , He Sisi 
Published: January 2024

AbstractSeamlessly integrating biosensors into clothing for personalized stress level monitoring, specifically targeting cortisol, a stress‐related compound found in sweat, shows great potential for efficiently monitoring and managing individual mental and physical well‐being. Nevertheless, realizing the fabric biosensor faces a notable challenge, as it involves the persistent obstacle of ensuring reusability and washability. Here, a fabric sensing system constructed by a fiber electrode for rapid and reliable cortisol detection with high repeatability, breathability, and stability is reported. The fibers are assembled from aligned carbon nanotubes and functionalized with a molecularly imprinted polymer containing redox‐active nanoreporters. Through washing with ethanol, the fabricated fabric sensor exhibits high reusability for over 100 cycles detecting cortisol. The key is the large active surface area and elaborately designed channel distance in the fiber electrode, ensuring a stable interface between the polymer and the fiber electrode. The resulting fabric sensing system presents convenient monitoring of cortisol levels, allowing assessment of stress levels to understand emotions and health condition better.
Polyamide Nanofilms through a Non‐Isothermal‐Controlled Interfacial Polymerization
Authors: Zhao Guang‐Jin , Li Lu‐Lu , Gao Hai‐Qi , Zhao Zhi‐Jian , Pang Zi‐Fan , Pei Chun‐Lei , Qu Zhou , Dong Liang‐Liang , Rao De‐Wei , Caro Jürgen , Meng Hong 
Published: January 2024

AbstractEfficient thin film composite polyamide (PA) membranes require optimization of interfacial polymerization (IP) process. However, it is challengeable owing to its ultrafast reaction rate coupled with mass and heat transfer, yielding heterogeneous PA membranes with low performance. Herein, a non‐isothermal‐controlled IP (NIIP) method is proposed to fabricate a highly permeable and selective PA membrane by engineering IP at the cryogenic aqueous phase (CAP) to achieve synchronous control of heat and mass transfer in the interfacial region. The CAP also enables the phase transition of the aqueous solution from the liquid to solid state, providing a more comprehensive understanding of the fundamental mechanisms involved in different phase states in the IP process. Consequently, the PA membrane exhibits excellent separation performance with ultrahigh water permeance (42.9 L m−2 h−1 bar−1) and antibiotic desalination efficiency (antibiotic/NaCl selectivity of 159.3). This study provides new insights for the in‐depth understanding of the precise mechanism linking IP to the performance of the targeting membrane.
Elastomeric Protein Bioactive Eutectogels for Topical Drug Delivery
Authors: Picchio Matías L. , Orellano María Soledad , Motta Maria Angela , Huck‐Iriart Cristián , Sánchez‐deAlcázar Daniel , López‐Domene Rocío , Martín‐García Beatriz , Larrañaga Aitor , Beloqui Ana , Mecerreyes David , Calderón Marcelo 
Published: January 2024

AbstractTherapeutic deep eutectic solvents (THEDES) are an emerging family of eutectic mixtures gaining increasing interest in the biomedical space. The immobilization of THEDES into polymer networks allows bioactive eutectogels to expand their application scope to topical drug delivery. Herein, this work presents the first set of elastomeric eutectogels constructed by supporting a therapeutic eutectic system with skin permeation ability in a protein scaffold dynamically crosslinked by a natural polyphenol. In this ionic eutectic, gelatin undergoes gelation through an unexpected mechanism in striking contrast with classical hydrogels, which is herein thoroughly studied. Interestingly, the polyphenol controls the conformation of the protein structure, enabling tuning up the mechanical and viscoelastic behavior of the dynamic eutectogel networks from elastic to hyperelastic. The resultant protein eutectogels exhibit strain‐hardening behavior, thermoreversible gel‐to‐sol transition, and excellent adhesive performance. Furthermore, these versatile materials retain the bioactivity of the liquid THEDES and favor skin occlusion, assisting the delivery of both hydrophilic and hydrophobic substances in ex vivo porcine skin in a time‐dependent penetration process. These ultrastretchable eutectogels show new interplays between protein scaffolds and eutectic mixtures, paving the way for innovative therapeutic soft materials.
DNA‐Mediated, On‐Membrane Sequential Assembly of Conjugated Polymer Nanoparticles for Sensitive Detection of Cell Surface Markers
Authors: Maeda Yuki , Nakamura Noriko , Ohta Seiichi 
Published: January 2024

AbstractFlow cytometry can provide detailed information about protein expression on cell surface and is, therefore, widely used in clinical testing. However, owing to the limited sensitivity of fluorescence signals, detection of low‐expression cell surface markers is challenging. The present report describes a DNA‐mediated, on‐membrane assembly of conjugated polymer nanoparticles (Pdots) that amplifies fluorescence signal from surface markers for sensitive detection via flow cytometry. Single‐stranded DNA (ssDNA)‐conjugated antibodies are first bound to cell surface markers, from which ssDNA‐modified Pdots are sequentially assembled using DNA hybridization. The use of DNA as a linker enables the distance‐controlled assembly of Pdots to prevent fluorescence quenching, whereas their on‐membrane sequential assembly allows amplification of the fluorescence signal without reducing binding ability of antibodies. Thus, two rounds of Pdot assembly achieve 31‐fold amplification of the fluorescence signal from CD19 on Nalm‐6 cells, which is 125‐fold brighter than that obtained using the conventional fluorescent dye‐based method. Moreover, the sequential assembly of 22 nm Pdots shows 24‐fold higher fluorescence than one‐step labeling with 81 nm Pdots, suggesting the advantage of the sequential assembly strategy in avoiding steric hindrance. The proposed method is expected to contribute to the sensitive detection of low‐expression surface markers for early and accurate diagnosis.
A Smart Responsive Nanotheranostic System for MRI of Tumor Response to Immunotherapy and Enhanced Synergism of Thermo‐Immunotherapy
Authors: Li Minghua , Lin Chao , Shen Aijun , Ma Xiaolong , Ni Jiong , Wu Jiaqi , Wang Wei , Wang Peijun , Gao Xiaolong 
Published: January 2024

AbstractThe challenges associated with the assessment of immune checkpoint inhibitor (ICI) therapy and low immunotherapy response observed in patients with poorly immunogenic tumors are addressed. A smart‐responsive nanoprobe MZF@Gd is synthetized based on magnetic resonance tuning (MRET). The nanoprobe is designed to be targeted and cleaved by granzyme B, a marker of cytotoxic T‐cell activation. MZF@Gd consisted of superparamagnetic Mn‐ and Zn‐doped ferrite magnetic nanoparticles (MZF‐MNPs) with a magnetocaloric effect and a paramagnetic contrast agent gadolinium‐diethylenetriamine penta‐acetic acid (Gd‐DTPA). These components are connected by the granzyme B substrate peptides. The activation of the T1WI signal through MRET, following the cleavage of the probe by granzyme B, enabled the accurate evaluation of the ICI therapeutic effect. Subsequently, an active‐targeting magnetothermally responsive nanotheranostic agent FA‐P(MZF@Gd/JQ1) is fabricated. This agent incorporated the MRET probe MZF@Gd, JQ‐1 (an inhibitor of PD‐L1), the thermosensitive copolymer PCL‐b‐P(MEO2MA‐co‐OEGMA), and folic acid on the surface. Nanotheranostic agents killed tumor cells through magnetocaloric effects, stimulated antitumor immunity, and regulated the drug release, thereby achieving a synergistic effect between hyperthermia and immunotherapy. Moreover, MZF@Gd released from the agent sensitively detected the presence of granzyme B at the tumor site, thereby enabling an effective assessment of therapeutic efficacy.
4D Printing of Adaptable “Living” Materials Based on Alkoxyamine Chemistry
Authors: Tran H.B. Duc , Vazquez‐Martel Clara , Catt Samantha O. , Jia Yixuan , Tsotsalas Manuel , Spiegel Christoph A. , Blasco Eva 
Published: January 2024

Abstract4D printing has emerged as a powerful strategy capable of revolutionizing additive manufacturing by enabling objects to dynamically transform overtime on demand. Despite significant progress, the full potential remains unrealized, particularly in the utilization of dynamic covalent chemistry. This study introduces a new approach using a multifunctional cross‐linker with alkoxyamine functionalities for 4D printing. Digital light processing (DLP) is employed for high‐resolution printing of complex objects. Leveraging alkoxyamine bonds' dynamic and living characteristics, the printed structures can be further modified through nitroxide‐mediated polymerization (NMP) using styrene and nitroxide exchange reactions (NER). The resulting “living” printed structures exhibit the unique ability to undergo both “growth” and “degrowth”, dynamically adapting their size as well as the reduced Young's Modulus across a wide range (770 kPa–1.2 GPa). The chain extension by NMP and softening by NER are carefully characterized by IR and EPR spectroscopy. The presented approach opens avenues for the development of 4D printed structures with complex adaptive systems, showcasing enormous potential in a wide range of fields.
Evolution of Grain Boundaries Promoted Hydrogen Production for Industrial‐Grade Current Density
Authors: Cheng Yu , Chen Huanyu , Zhang Lifang , Xu Xinnan , Cheng Huili , Yan Chenglin , Qian Tao 
Published: January 2024

AbstractThe development of efficient and durable high‐current‐density hydrogen production electrocatalysts is crucial for the large‐scale production of green hydrogen and the early realization of hydrogen economic blueprint. Herein, the evolution of grain boundaries through Cu‐mediated NiMo bimetallic oxides (MCu‐BNiMo), which leading to the high efficiency of electrocatalyst for hydrogen evolution process (HER) in industrial‐grade current density, is successfully driven. The optimal MCu0.10‐BNiMo demonstrates ultrahigh current density (>2 A cm−2) at a smaller overpotential in 1 m KOH (572 mV), than that of BNiMo, which does not have lattice strain. Experimental and theoretical calculations reveal that MCu0.10‐BNiMo with optimal lattice strain generated more electrophilic Mo sites with partial oxidation owing to accelerated charge transfer from Cu to Mo, which lowers the energy barriers for H* adsorption. These synergistic effects lead to the enhanced HER performance of MCu0.10‐BNiMo. More importantly, industrial application of MCu0.10‐BNiMo operated in alkaline electrolytic cell is also determined, with its current density reached 0.5 A cm−2 at 2.12 V and 0.1 A cm−2 at 1.79 V, which is nearly five‐fold that of the state‐of‐the‐art HER electrocatalyst Pt/C. The strategy provides valuable insights for achieving industrial‐scale hydrogen production through a highly efficient HER electrocatalyst.
A Science Friction Story: Molecular Interactions in Semiflexible Polymer Networks
Authors: Mollenkopf Paul , Prascevic Dusan , Glaser Martin , Smith David M. , Schnauß Jörg 
Published: January 2024

AbstractEstablished model theories, developed to capture the mechanical behavior of soft, complex materials composed of semiflexible polymers, assume that entropic interactions between filaments are primarily responsible for determining the mechanical response. In recent studies, the generally accepted tube model has been challenged in terms of this basic assumption about filament–filament interactions, but also because of its predictions regarding the frequency dependence of the elastic modulus in the intermediate frequency regime. A central question is how molecular interactions and friction between network constituents influence the rheological response of isotropic entangled networks of semiflexible polymers. It has been previously shown that friction forces between aligned pairs of actin filaments are not negligible. Here, the influence of friction forces and attractive interactions on network rheology is systematically investigated by means of targeted surface modification. It is shown that these forces have a qualitative and quantitative influence on the viscoelastic properties of semiflexible polymer networks and contribute to their response to nonlinear deformations. By comparing two polymer model systems with respect to their surface compositions, a possible explanation is given about the origin of acting forces on a molecular level.