bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic analysis of Anopheles gambiae from Benin reveals overexpression of salivary and cuticular proteins associated with cross-resistance to pyrethroids and organophosphates
Authors: Saizonou Helga , Impoinvil Lucy Mackenzie , Derilus Dieunel , Omoke Diana , Okeyo Stephen , Dada Nsa , Mulder Nicola , Lenhart Audrey , Ochomo Eric , Djogbenou Luc S. 
Published: October 2023

Abstract

Background
Insecticide resistance (IR) is one of the major threats to malaria vector control programs in endemic countries. However, the mechanisms underlying insecticide resistance are poorly understood. Thus, investigating gene expression patterns related to IR can offer important insights into the molecular basis of IR in mosquitoes. In this study, RNA-Seq was used to characterize gene expression in Anopheles gambiae surviving exposure to pyrethroids (deltamethrin, alphacypermethrin) and an organophosphate (pirimiphos-methyl).
Results
Larvae of An. gambiae s.s. collected from Bassila and Djougou in Benin were reared to adulthood and phenotyped for IR using a modified CDC intensity bottle bioassay. The results showed that mosquitoes from Djougou were more resistant to pyrethroids (5X deltamethrin: 51.7% mortality; 2X alphacypermethrin: 47.4%) than Bassila (1X deltamethrin: 70.7%; 1X alphacypermethrin: 77.7%), while the latter were more resistant to pirimiphos-methyl (1.5X: 48.3% in Bassila and 1X: 21.5% in Djougou). RNA-seq was then conducted on resistant mosquitoes, non-exposed mosquitoes from the same locations, and laboratory-susceptible An. gambiae s.s. Kisumu strain. The results showed overexpression of detoxification genes, including cytochrome P450s (CYP12F2, CYP12F3, CYP4H15, CYP4H17, CYP6Z3, CYP9K1, CYP4G16, and CYP4D17), carboxylesterase genes (COEJHE5E, COE22933) and glutathione S-transferases (GSTE2 and GSTMS3) in all three resistant mosquito groups analyzed. Genes encoding cuticular proteins (CPR130, CPR10, CPR15, CPR16, CPR127, CPAP3-C, CPAP3-B, and CPR76) were also overexpressed in all the resistant groups, indicating their potential role in cross resistance in An. gambiae. Salivary gland protein genes related to ‘salivary cysteine-rich peptide’ and ‘salivary secreted mucin 3’ were also over-expressed and shared across all resistant groups.
Conclusion
Our results suggest that in addition to metabolic enzymes, cuticular and salivary gland proteins could play an important role in cross-resistance to multiple classes of insecticides in Benin. These genes warrant further investigation to validate their functional role in An. gambiae resistance to insecticides.
Nivo-BeGEV как подготовка к трансплантации аутологичных гемопоэтических стволовых клеток при рецидивах и рефрактерном течении классической лимфомы Ходжкина: результаты многоцентрового проспективного клинического исследования
Authors: Мангасарова Яна Константиновна , Моисеева Т. Н. , Марголин О. В. , Горенкова Л. Г. , Нестерова Е. С. , Бабаева Ф. Э. , Багова М. О. , Фастова Е. А. , Абдурашидова Р. Р. , Аль-Ради Л. С. , Дорохина Е. И. , Володичева Е. М. , Лапин В. А. , Самойлова О. С. , Кравченко С. К. , Магомедова А. У. , Звонков Е. Е. 
Published: October 2023

Aim. To assess efficacy and safety of the Nivo-BeGEV (nivolumab combined with bendamustine, gemcitabine, and vinorelbine) immunochemotherapy in patients with relapsed/refractory (r/r) classical Hodgkin lymphoma (cHL) selected as candidates for autologous hematopoietic stem cell transplantation (auto-HSCT).
Materials & Methods. During 2018–2022, the study enrolled 51 r/r cHL patients treated with the Nivo-BeGEV immunochemotherapy. The median age was 38 years (range 19–57 years). There were 30 men and 21 women. PET-CT was performed to assess the response according to the LYRIC criteria. Safety and tolerability were analyzed by registering adverse events in line with the NCI CTCAE criteria, version 5.
Results. The median follow-up was 12 months (range 3–54 months). Complete remissions were reported in 100 % of cases. An early relapse was observed in 1 (2 %) patient. The 2-year overall and progression-free survivals were 100 % and 93 %, respectively. During Nivo-BeGEV administration, severe adverse events of grade 3/4 developed in 6 (13 %) out of 51 patients.
Conclusion. The results of this multi-center prospective clinical study of the Nivo-BeGEV immunochemotherapy used as preparation for auto-HSCT in r/r cHL patients showed high efficacy irrespective of prior drug chemotherapy and its duration with an acceptable toxicity profile.
Эффективность трансплантации аутологичных гемопоэтических стволовых клеток у больных множественной миеломой старше 60 лет
Authors: Кострома Иван Иванович , Юдина В. А. , Сабитова Р. Р. , Степченкова Е. С. , Чубукина Ж. В. , Бессмельцев С. С. , Сидоркевич С. В. , Грицаев С. В. 
Published: October 2023

Aim. To compare toxicity and efficacy of high-dose melphalan chemotherapy with subsequent autologous hematopoietic stem cell transplantation (auto-HSCT) in multiple myeloma (MM) patients aged under and over 60 years.
Materials & Methods. The retrospective analysis was conducted on the data of 107 MM patients, 78 of them were aged under 60 years (median 54 years), and 29 of them were aged 61 years and older (median 63 years). All patients received auto-HSCT in the period of 2017–2022. Single and tandem auto-HSCT were performed in 92 and 15 patients, respectively. Patients with tandem auto-HSCT (n = 15), lost to follow-up patients (n = 8), and patients who died during early post-transplant period (n = 4) were excluded from survival analysis. Survival rates were calculated based on the date of auto-HSCT.
Results. A comparative evaluation of the results in two age groups showed a significant difference in the number of patients treated with ixazomib during the induction period (р = 0.019) and cyclophosphamide 3 g/m2 as part of auto-HSC mobilization (р = 0.014), as well as 200 or 140 mg/m2 melphalan as part of conditioning regimen (р = 0.039 and р = 0.009, respectively). With a follow-up median of 13 months (range 1–57 months), the median progression-free survival in the groups ≤ 60 years vs. > 60 years was 32 and 47 months, respectively (hazard ratio [HR] 0.688; 95% confidence interval [95% CI] 0.270–1.754; p = 0.704). The median overall survival in patients aged under 60 years appeared to be 57 months, it was not reached in patients aged 61 years and older (HR 0.689; 95% CI 0.169–2.803; р = 0.577).
Conclusion. The results of the study suggest that all newly diagnosed MM patients aged under 70 years should be regarded as being eligible for auto-HSCT.
Множественная миелома, осложненная костными плазмоцитомами: патогенез, клиническая картина, лечебные подходы (обзор литературы)
Authors: Мамаева Елизавета Андреевна , Соловьева М. В. , Менделеева Л. П. 
Published: October 2023

Bone plasmacytoma is a malignant neoplasm consisting of plasma cells. It develops in the medullary cavities of the skeletal bones. The tumor can destroy bone cortex and proliferate into the surrounding tissues. In contrast to bone plasmacytomas, extramedullary plasmacytomas occur as a result of hematogenous dissemination in various tissues and organs. Based on literature data, the incidence of bone plasmacytomas at multiple myeloma (MM) onset is 7.0 % to 32.5 %, and at relapsed/progression ММ stages it is 9.0 % to 27.4 %. During bone plasmacytoma development, tumor cells acquire a number of new features: expression of adhesion molecules is decreased, new cytogenetic aberrations occur, autocrine secretion and neoangiogenesis are increased. The clinical course of MM complicated by bone plasmacytomas is characterized by minimal bone marrow damage, hemoglobin concentration within reference range, and decreased values of β2-microglobulin, paraprotein, calcium, and lactate dehydrogenase. Acute renal failure and immunoparesis are rare, early MM stages predominate. In literature, the MM form with multiple bone plasmacytomas is referred to as ‘macrofocal MM’. Survival rates of MM patients with bone plasmacytomas are at the intermediate level in terms of prognosis. The MM patients without plasmacytomas have the most favorable prognosis, whereas the MM patients with extramedullary plasmacytomas have the poorest prognosis. There is no unified approach to the treatment of MM complicated by bone plasmacytomas. There are no randomized prospective clinical studies on the efficacy of treating it. A successful use of proteasome inhibitors and immunomodulatory drugs was reported based on a small number of MM cases with plasmacytomas. Some studies proved the efficacy of auto-HSCT in this MM form. Bone plasmacytomas are treated with radiotherapy mainly after systemic chemotherapy.
Evaluation of statistical downscaling model's performance in projecting future climate change scenarios
Authors: Shukla Rituraj , Khare Deepak , Kumar Dwivedi Anuj , Rudra Ramesh Pal , Palmate Santosh S. , Ojha C. S. P. , Singh Vijay P. 
Published: October 2023

Abstract

Statistical downscaling (SD) is preferable to dynamic downscaling to derive local-scale climate change information from large-scale datasets. Many statistical downscaling models are available these days, but comparison of their performance is still inadequately addressed for choosing a reliable SD model. Thus, it is desirable to compare the performance of SD models to ensure their adaptability in future climate studies. In this study, a statistical downscaling model (SDSM) or multi-linear regression and the Least Square Support Vector Machine (LS-SVM) were used to do downscaling and compare the results with those obtained from general circulation model (GCM) for identifying the best SD model for the Indira Sagar Canal Command area located in Madhya Pradesh, India. The GCM, Hadley Centre Coupled Model version 3 (HadCM3), was utilized to extract and downscale precipitation, maximum temperature (Tmax), and minimum temperature (Tmin) for 1961–2001 and then for 2001–2099. Before future projections, both SD models were initially calibrated (1961–1990) and validated (1991–2001) to evaluate their performance for precipitation and temperature variables at all gauge stations, namely Barwani, East Nimar, and West Nimar. Results showed that the precipitation trend was under-predicted owing to large errors in downscaling, while temperature was over-predicted by SD models.
Target urgent study of fresh frozen sections to define the surgical margin status during radical prostatectomy
Authors: Kotov S. V. , Byadretdinov I. Sh. , Guspanov R. I. , Pulbere S. A. , Yusufov A. G. 
Published: October 2023

Introduction. Radical prostatectomy (RP) is the "gold standard" treatment for patients with prostate cancer (PCa). Obviously, one of the predictors actively studied of biochemical recurrence is the presence of a positive surgical margin.Objective. To develop and initially evaluate the techniques of targeted urgent intraoperative histological study of fresh frozen sections during radical prostatectomy.Materials & methods. This study was carried out within the framework of the academic research at the University Clinic of Urology, Pirogov Medical University. We conducted a prospective clinical study of fresh frozen tissue samples during laparoscopic RP (LPR). The subject of the investigation are 112 patients with PCa underwent LRP (extra- and transperitoneal approaches) by one surgeon in 2021 – 2022. Group A involves 62 patients underwent intraoperative examination of fresh frozen sections based on preoperative data (MRI, FUSION biopsy). Group B (control retrospective group) consists of 50 patients with standard surgery without fresh frozen sections.Results. The accumulation of new data allows us to say that the presence of an oncological process was found in the gross specimen obtained by performing cito histology in 42 (67.7%) patients. A primary positive margin was found in 12 (19.4%). Compared to group B, a positive surgical margin was found in 7 (14%). Migration of surgical margin status from initially negative to finally positive margin found in 2 (3.3%). Migration of status from primary positive to negative surgical margin was found in 2 (3.3%). The specificity of the method (true negative margin) was determined in 48 of 50 patients (96.1%), and the sensitivity (true positive margin) was determined in 10 of 12 patients (83.3%). The average time to complete the technique of rapid histological study was 27 minutes.Conclusion. Since the work carried out, we have come to the following conclusion that the obtained results indicate the effectiveness and expediency of using targeted urgent histological study during RP.
Features of blood supply of urethral tissues in women with primary urethral pain syndrome
Authors: Kuyarov A. S. , Kiseleva E. B. , Matveev L. A. , Dudenkova V. V. , Strelzova О. S. 
Published: October 2023

Introduction. Primary urethral pain syndrome (PUPS) is a part of chronic primary pelvic pain syndrome with predominant localisation of pain in the urethra. The pathogenesis of PUPS has not been adequately studied and there are no unified guidelines for the prevention and treatment of this condition. It is known that one of the main factors in the development of chronic pain is a violation of regional blood flow.Objective. To identify the features of blood supply of urethral tissues, as well as to assess the concomitant changes in the connective tissue in patients with PUPS.Materials & methods. The study included 98 patients: 78 patients with primary urethral pain syndrome (group "PUPS"), without clinical manifestations of inflammation; 20 patients with stones of the upper urinary tract without pyelonephritis, in which the urethra is taken as the norm (group "N"). The hemodynamics of the urethral tissues was studied using transvaginal ultrasound (TVUS) and laser Doppler flowmetry (LDF). Changes in the blood supply to organs often lead to disruption of tissue structure; therefore, in vivo cross-polarisation optical coherence tomography (CP OCT) and ex vivo nonlinear confocal microscopy were included in the study.Results. In the PUPS group, the LDF method revealed a violation of the neurogenic regulation of the tone of the microcirculatory vascular wall of the urethral tissues with a predominance of passive regulatory components. The analysis of the LDF parameters by the method of principal components made it possible to establish the division of data into several clusters, which indicates a different pathogenesis of microcirculation disorders in the tissues of the urethra. The TVUS method in the PUPS group revealed an increase in resistance to blood flow in the blood vessels of the urethra and paraurethral plexus, blood stasis in the veins of the small pelvis in 19% of patients. Violation of the urethral blood flow leads to structural changes in tissues: using nonlinear microscopy, it was found that with PUPS in the urethral connective tissue, collagen fibres are quantitatively larger relative to elastic ones, they are more uniform in thickness, and have a co-directional arrangement. These data correlate with the results of an intravital CP OCT study, during which fibrosis of the subepithelial connective tissue structures was revealed.Conclusion. TVUS and LDF made it possible to reveal the presence of microcirculatory and, as a result, structural changes in urethral tissues in vivo. The inclusion of these methods in the diagnostic algorithm will provide more information about the main links in the pathogenesis of PUPS.
Use of the Smart Excretion Care System Linked to Electronic Medical Records to Alleviate Nursing Burden and Enhance Patient Convenience: Mixed Methods Study
Authors: Moon Hui-Woun , Park Da Som Me , Jung Se Young 
Published: October 2023

Background
The surge in older demographics has inevitably resulted in a heightened demand for health care, and a shortage of nursing staff is impending. Consequently, there is a growing demand for the development of nursing robots to assist patients with urinary and bowel elimination. However, no study has examined nurses’ opinions of smart devices that provide integrated nursing for patients’ urinary and bowel elimination needs.

Objective
This study aimed to evaluate the feasibility of the Smart Excretion Care System tethered to electronic medical records in a tertiary hospital and community care setting and discuss the anticipated reductions in the burden of nursing care.

Methods
Focus group interviews were conducted using the Consolidated Criteria for Reporting Qualitative Research (COREQ) guidelines. The interviews were conducted in March 2021 and involved 67 nurses who had worked at Seoul National University Bundang Hospital for more than 1 year and had experience in assisting patients with excretion care. Data were collected using purposive and snowball sampling methods.

Results
A total of four themes relevant to the Smart Excretion Care System were found: (1) expected reductions in the burden of nursing care, (2) applicable indications (by departments and diseases), (3) preferred features/functions, and (4) expected benefits of using the Smart Excretion Care System in clinical facilities. Nurses from comprehensive nursing care wards had the highest burden when it came to excretion care. It was a common opinion that the Smart Excretion Care System would be very useful in intensive care units and should be applied first to patients with stroke or dementia.

Conclusions
Excretion care is one of the most burdensome tasks for nurses, increasing their workload. The development of the Smart Excretion Care System as a digital health intervention could help improve nurses’ work efficiency, reduce their burden, and extend to caregivers and guardians.
Usability of the Experience Sampling Method in Specialized Mental Health Care: Pilot Evaluation Study
Authors: Weermeijer Jeroen Dennis Merlijn , Wampers Martien , de Thurah Lena , Bonnier Rafaël , Piot Maarten , Kuppens Peter , Myin-Germeys Inez , Kiekens Glenn 
Published: October 2023

Background
Mental health problems occur in interactions in daily life. Yet, it is challenging to bring contextual information into the therapy room. The experience sampling method (ESM) may facilitate this by assessing clients’ thoughts, feelings, symptoms, and behaviors as they are experienced in everyday life. However, the ESM is still primarily used in research settings, with little uptake in clinical practice. One aspect that may facilitate clinical implementation concerns the use of ESM protocols, which involves providing practitioners with ready-to-use ESM questionnaires, sampling schemes, visualizations, and training.

Objective
This pilot study’s objective was to evaluate the usability of an ESM protocol for using the ESM in a specialized mental health care setting.

Methods
We created the ESM protocol using the m-Path software platform and tested its usability in clinical practice. The ESM protocol consists of a dashboard for practitioners (ie, including the setup of the template and data visualizations) and an app for clients (ie, for completing the ESM questionnaires). A total of 8 practitioners and 17 clients used the ESM in practice between December 1, 2020, and July 31, 2021. Usability was assessed using questionnaires, ESM compliance rates, and semistructured interviews.

Results
The usability was overall rated reasonable to good by practitioners (mean scores of usability items ranging from 5.33, SD 0.91, to 6.06, SD 0.73, on a scale ranging from 1 to 7). However, practitioners expressed difficulty in personalizing the template and reported insufficient guidelines on how to use the ESM in clinical practice. On average, clients completed 55% (SD 25%) of the ESM questionnaires. They rated the usability as reasonable to good, but their scores were slightly lower and more variable than those of the practitioners (mean scores of usability items ranging from 4.18, SD 1.70, to 5.94, SD 1.50 on a scale ranging from 1 to 7). Clients also voiced several concerns over the piloted ESM template, with some indicating no interest in the continued use of the ESM.

Conclusions
The findings suggest that using an ESM protocol may facilitate the implementation of the ESM as a mobile health assessment tool in psychiatry. However, additional adaptions should be made before further implementation. Adaptions include providing training on personalizing questionnaires, adding additional sampling scheme formats as well as an open-text field, and creating a dynamic data visualization interface. Future studies should also identify factors determining the suitability of the ESM for specific treatment goals among different client populations.
International Legal Regulation of the Problem of Protecting the Environment from Radioactive Contamination
Authors: Paramuzova O. G. 
Published: October 2023

This article deals with modern actual international legal problems of ecological and nuclear law. In the frames of conducted research the special emphasis was made on, that in the modern interstate system is required a new codification of common international law norms, as well as initiation of the process of regional rulemaking.Aim. The main intention of research is search for solutions the problems protecting the environment from radioactive contamination in the context of increasing the effectiveness of the mechanism of international legal regulation in the epoch of modern international relations.Tasks. To determine the relevance and specificity the actual international legal problems of protecting the environment from radioactive contamination in the context of increasing the effectiveness of the mechanism of international legal regulation in the 21th century.Methods. Within the framework of this study, using the methods of studying normative legal acts and international legal doctrines, as well as content analysis, the features of the functioning of the mechanism of international legal regulation in the field of environmental protection from radioactive contamination are revealed.Results. During the study, the author concludes, that in the light of today’s threats and challenges, it is necessary to try to develop legally significant ways out of the crisis associated with solving international legal environmental problems of preventing nuclear damage, as well as eliminating and reducing the negative consequences of its impact on the state of the natural environment. On of the justified ways to solve threes problems is the reform of international legal consciousness in general and in the areas of international environment law and nuclear law, in particular. In the modern interstate system, it seem that a new codification of the norms of general international law is to required, as well as the initiation of a process of regional law-making.Conclusion. Summing up the results of the study, the author of investigation gives his findings and conclusions regarding the resolution of these problems in this area of international legal regulation. In particular, the article determines the validity of the assertion that in the context of modern threats and exports to international peace and security in the 21st century, a qualitative renewal of international legal consciousness, its enrichment and nourishment with new scientific ideas and well-thought-out concepts that can bring international law out of crisis since the legal consciousness that exists today does not meet modern realities and, relying on it, it will be extremely difficult to ensure the effectiveness of the well-coordinated work out of international interstate system.