bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of succinate dehydrogenase sensitizes cyclin E‐driven ovarian cancer to CDK inhibition
Authors: Guo Ting , Gu Chao , Chen Xiuying , Kang Yu , Li Bin , Xu Congjian 
Published: October 2023

AbstractAim: High‐grade serous ovarian cancer (HGS‐OvCa) is characterized by widespread CCNE1 amplification. Current treatments lack specificity to target Cyclin E‐driven OvCa. Methods: By in silico analysis of the TCGA OvCa dataset we searched association between genes involved in glucose metabolism and cell cycle control. Metabolic shift was studied in Cyclin E‐driven OvCa cells treated with CDK inhibition (CDKi). Genetic and pharmaceutical inhibition of succinate dehydrogenase (SDH) was tested in combination with CDKi. Results: OvCa patients with CCNE1 amplification could be divided by concomitant SDHA amplification. A2780 OvCa cells were similar to the Cyclin E‐driven and SDHA neutral genotype. CDKi in A2780 cells using Dinaciclib resulted in compensatory enhancement of tricarboxylicacid cycle (TCA) cycle activity. Combined blockade of CDK and SDH, both genetically and pharmaceutically, showed synergy and resulted in inhibited proliferation, migration, invasion and migration in A2780 cells. The combined inhibition did not further alter cell cycle population, but induced apoptosis of A2780 cells. Conclusion: Cyclin E‐driven OvCa cells appeared addicted to glucose metabolism via TCA. Combined CDKi with modalities targeting TCA, like SDHA inhibition showed promising effects for this genotype. © 2016 BioFactors, 42(2):171–178, 2016
Fabrication of Chitosan‐Gold Nanoshells for γ‐Aminobutyric Acid Detection as a Surface‐enhanced Raman Scattering Substrate
Authors: Lim Jae‐Wook , Kang Ik‐Joong 
Published: October 2023

A surface‐enhanced Raman scattering (SERS) principle‐based brain‐probing optical fiber was produced to analyze changes in the concentration of γ‐aminobutyric acid (GABA). The procedure was carried out within the GABA administration concentration range of 5–30 mM for the patients who should take cranial medicines for GABA components of less than a normal range. The optical fiber, of which the surface had been reformed, was prepared by treatment with chitosan‐gold nanoshell particles, which were synthesized by using an ionic interaction. The treated fiber was subsequently combined with GABA, for the purpose of analyzing its GABA concentration. The calibration curve of the SERS sensitivity (counts) as determined by the concentration levels of GABA adsorbed onto the chitosan‐gold nanoshell without attachment to the optical fiber was as follows: y = 0.2x
2 − 0.6743x + 419.8 (
R
2
= 0.9874), while the corresponding calibration curve of the SERS sensitivity (counts) with attachment to optical fiber was as follows: y = 7.4057x
2 + 93.68x + 1851.4 (
R
2
= 0.9472). Based on the aforementioned two sets of data, it was confirmed that the analysis of GABA using optical fiber had a higher level of sensitivity compared to its analysis in the absence of optical fiber.
President's Page
Authors: Connaway Lynn Silipigni 
Published: October 2023

EDITOR'S SUMMARYASIS&T president Lynn Silipigni Connaway announced the new executive director of ASIS&T, Lydia Middleton. Lydia joined ASIS&T ready to lead after Dick Hill's 27 years of service as executive director. Also announced are the keynote speakers for the 2017 Annual Meeting: Richard Marks, head of PlayStation Magic Lab at Sony Interactive Entertainment, and William Powers, best‐selling author and research scientist at MIT Media Lab. The Annual Meeting will also have an 80th Anniversary Welcome Reception and panel session, and work is underway for an interactive history exhibit at the meeting, coordinated by Kathryn La Barre. Lynn Silipigni Connaway was invited to spend April 26 through May 7 in Hong Kong as a visiting professor working with the master of library and information management program at the University of Hong Kong. Connaway and past president Nadia Caidi also attended the Council for Scientific Society Presidents in Washington, D.C., May 2017, and ASIS&T partnered with the American Association for the Advancement of Science and the Earth Day Network for the March for Science.
Photo‐Induced C−H Methylation Reactions
Authors: Li Qian‐Yu , He Yuhang , Lin Yu‐Mei , Gong Lei 
Published: October 2023

AbstractDirect C−H methylation is a highly valuable approach for introducing methyl groups into organic molecules, particularly in pharmaceutical chemistry. Among the various methodologies available, photo‐induced methylation stands out as an exceptional choice due to its mild reaction conditions, energy efficiency, and compatibility with functional groups. This article offers a comprehensive review of photochemical strategies employed for the direct and selective methylation of C(sp3)−H, C(sp2)−H, and C(sp)−H bonds in various organic molecules. The discussed methodologies encompass transition‐metal‐based photocatalysis, organophotocatalysis, as well as other metal‐free approaches, including electron donor‐acceptor (EDA)‐enabled transformations. Importantly, a wide range of easily accessible agents such as tert‐butyl peroxide, methanol, DMSO, methyl tert‐butyl ether, TsOMe, N‐(acetoxy)phthalimide, acetic acid, methyl halides, and even methane can serve as effective methylating reagents for modifying diverse targets. These advancements in photochemical C−H methylation are anticipated to drive further progress in the fields of organic synthesis, photocatalysis, and pharmaceutical development, opening up exciting avenues for creating novel organic molecules and discovering new drug compounds.
Shedding Light on Highly Emissive 1,4‐Dihydropyrrolo[3,2‐b]pyrrole Derivatives: Synthesis and Aggregate‐Dependent Emission
Authors: Abatti Priscila Pazini , Decarli Nícolas Oliveira , Gogoc Szymon , Data Przemyslaw , Bechtold Ivan H. , Westphal Eduard , Gallardo. Hugo 
Published: October 2023

AbstractThree tetraaryl‐1,4‐dihydropyrrolo[3,2‐b]pyrrole derivatives containing different number of long alkoxy chains (2, 4 and 6) were synthesized, characterized and applied in Organic Light Emitting Diodes (OLEDs). The compounds showed good emission properties with Photoluminescence Quantum Yields (PLQYs) higher than 80 % in solution and 50 % in solid state (thin film). The solvatochromism results revealed a pronounced vibronic emission in methylcyclohexane and toluene, characterized by two distinct sharp emission peaks and a small redshift in the following order: methylcyclohexane>toluene>dichloromethane>tetrahydrofuran>acetonitrile. Also, the compounds formed aggregates with redshifted emission, which can be attributed to excimer formation. This phenomenon was observed in solutions containing 90 % water and with the concentration variation in methylcyclohexane (MCH). Compounds with a greater number of peripheral chains showed the capacity to keep hexagonal columnar organization in films after fast cooling from liquid state. OLEDs fabricated with these compounds showed turn‐on voltages lower than 4.0 V, with luminance higher than 1400 cd m−2, electroluminescence spectra with Full Width at Half Maximum lower than 70 nm and maximum External Quantum Efficiency between 7.2 % and 4.3 %. Overall, this shows that the 1,4‐dihydropyrrolo[3,2‐b]pyrrole moiety is promising for applications where luminescence is paramount, as in organic light‐emitting devices.
Phospholipase D Immobilization on Lignin Nanoparticles for Enzymatic Transformation of Phospholipids
Authors: Rossato Letizia Anna Maria , Morsali Mohammad , Ruffini Eleonora , Bertuzzi Pietro , Serra Stefano , D'Arrigo Paola , Sipponen Mika 
Published: October 2023

AbstractLignin nanoparticles (LNPs) are promising components for various materials, given their controllable particle size and spherical shape. However, their origin from supramolecular aggregation has limited the applicability of LNPs as recoverable templates for immobilization of enzymes. In this study, we show that stabilized LNPs are highly promising for the immobilization of phospholipase D (PLD), the enzyme involved in the biocatalytic production of high‐value polar head modified phospholipids of commercial interest, phosphatidylglycerol, phosphatidylserine and phosphatidylethanolamine. Starting from hydroxymethylated lignin, LNPs were prepared and successively hydrothermally treated to obtain c‐HLNPs with high resistance to organic solvents and a wide range of pH values, covering the conditions for enzymatic reactions and enzyme recovery. The immobilization of PLD on c‐HLNPs (PLD‐c‐HLNPs) was achieved through direct adsorption. We then successfully exploited this new enzymatic preparation in the preparation of pure polar head modified phospholipids with high yields (60–90 %). Furthermore, the high stability of PLD‐c‐HLNPs allows recycling for a number of reactions with appreciable maintenance of its catalytic activity. Thus, PLD‐c‐HLNPs can be regarded as a new, chemically stable, recyclable and user‐friendly biocatalyst, based on a biobased inexpensive scaffold, to be employed in sustainable chemical processes for synthesis of value‐added phospholipids.
Acinic cell carcinoma mimicking Warthin's tumor: A diagnostic challenge on fine‐needle cytology
Authors: Yaprak Bayrak Busra , Ozturk Murat 
Published: October 2023

AbstractThe cytologic diagnosis of acinic cell carcinoma (ACC) can be challenging due to its polymorphous appearance and sharing cytomorphologic characteristics with other benign and malignant neoplasms as well as non‐neoplastic diseases, even though various histomorphological aspects of ACC have been documented. We presented a 39‐year‐old female patient applied with right pre‐auricular parotid swelling spreading infra‐auricular region which was gradually increased in size for 3 months. Ultrasonographic examination revealed hypoechoic well‐circumscribed mass with 17 × 22 × 29 mm size. Magnetic resonance imaging revealed intra‐parotid solid lesion with cystic areas, slightly hypointense on T1 and hyperintense on T2 weighted images. The mass was pushing the retromandibular vein medially, still lateral to it in the caudal images, but in dumbbell‐shape spreading through parapharyngeal space in superiorly cranial images. Fine‐needle aspiration cytology was also performed with guidance of ultrasonography. The cytological examination of the lesion was characterized by the predominance of heterogeneous lymphoid cells, clusters of epithelial cells with a variety of cytologic appearances, including granular, transparent, vacuolated, and oncocytic, and the presence of numerous naked nuclei with a protein‐like foamy background. Due to intense lymphocytic inflammation, it was considered as benign primary parotid tumor such as Warthin's tumor. The excision material was examined histopathologically. Immunohistochemical analysis showed that this carcinoma was positive for DOG1, SOX10, cytokeratin 7 and negative for mammaglobin. This salivary gland tumor was reported as a rare variant of ACC with lymphoid‐rich stroma. To improve the diagnostic accuracy, various morphological aspects of ACC should be considered in the pathological practice.
Examination of the Influence by the Stimulation Coil Arrangement and the Shape of the Stimulation Object in Transcranial Magnetic Stimulation Using a Model
Authors: OIWA KOSUKE , YOSHIMATSU HIROTAKA , NURUKI ATSUO , YUNOKUCHI KAZUTOMO , TAMARI YOZO , JIMBO YASUHIKO 
Published: October 2023

SUMMARYMagnetic stimulation is a way of stimulating nervous and muscular tissue with an electric field induced by a stimulation coil and is widely used in evaluation of the nervous system and in the field of exercise physiology. The focality and the deep stimulation efficiency of magnetic stimulation are affected by the coil arrangement and the shape of the stimulated object, since the induced electric field distribution is varied by these factors. In this study, we used a five‐layer head model and estimated the induced electric field distribution by calculation. The results indicate that the induced electric field distribution is mostly affected by the boundaries between tissues with different conductivities.
Determinants of survival in patients with chronic heart failure: a population‐based study in Reggio Emilia, Italy
Authors: Pedroni Cristina , Djuric Olivera , Mancuso Pamela , Navazio Alessandro , Pinotti Mirco , Greci Marina , Giorgi Rossi Paolo 
Published: October 2023

AbstractAimsWe aim to monitor and improve the quality of the heart failure (HF) integrated assistance model defined by national and regional guidelines and implemented in the province of Reggio Emilia, Italy. Specific aims of the audit were to estimate the prevalence of HF, describe the characteristics of patients with HF and the rate of patients enrolled in the integrated care treated in primary care, and identify socioeconomic and geographic determinants of the 4‐year survival of these patients.Methods and resultsRetrospective analysis of a cohort of prevalent cases of HF, diagnosed before 31 December 2015 in Reggio Emilia, Italy, alive on 1 January 2016, and residing at the time of diagnosis on the provincial territory. Age and sex‐adjusted prevalence of HF by area of residence were calculated according to the standard European population 2013. Patients were followed until death or 31 December 2019, whatever came first. The outcome measure of the study was four‐year case fatality. Cox proportional hazards models, adjusted for age, sex, and duration of disease were used to determine the association between socio‐geographic factors and death. The 4‐year case‐fatality rate was 36.7%, and it was the highest in the mountains (50.8%) compared with hills (34.6%), lowland (35.4%) and city (37.7%). The prevalence of HF was the lowest in the mountain [149.9, 95% confidence interval (CI) 112.1–187.7] and the highest in the lowland (340.8, 95% CI 308.7–372.9) and city (308, 95% CI 276.0–321.2). Patients living in the mountains had a lower deprivation index, and fewer hospitalizations prior to official diagnosis, although these characteristics were not statistically significant determinants of HF death in multivariate analysis. Behavioural (smoking and obesity) and socio‐geographic characteristics (educational level, deprivation index and area of residence) were not significantly associated with mortality in both univariable and multivariable analysis; however, patients who live in mountains (hazard ratio 1.10, 95% CI 0.73–1.66) or hills (hazard ratio 1.11, 95% CI 0.90–1.37) had a slightly higher risk of death than those living in the city. Only 197 (12.1%) of patients in the cohort were enrolled in the integrated care pathway over the course of 4 years.ConclusionsAlthough clinical determinants outweigh the geographic and behavioural disparities in the survival of patients with CHF treated in primary care, effective prevention strategies are needed to address environmental and socio‐geographic inequalities in access to primary care and to hasten equitable linkage to integrated care.
Single‐cell multi‐omics analysis of COVID‐19 patients with pre‐existing autoimmune diseases shows aberrant immune responses to infection
Authors: Barmada Anis , Handfield Louis‐François , Godoy‐Tena Gerard , de la Calle‐Fabregat Carlos , Ciudad Laura , Arutyunyan Anna , Andrés‐León Eduardo , Hoo Regina , Porter Tarryn , Oszlanczi Agnes , Richardson Laura , Calero‐Nieto Fernando J. , Wilson Nicola K. , Marchese Domenica , Sancho‐Serra Carmen , Carrillo Jorge , Presas‐Rodríguez Silvia , Ramo‐Tello Cristina , Ruiz‐Sanmartin Adolfo , Ferrer Ricard , Ruiz‐Rodriguez Juan Carlos , Martínez‐Gallo Mónica , Munera‐Campos Mónica , Carrascosa Jose Manuel , Göttgens Berthold , Heyn Holger , Prigmore Elena , Casafont‐Solé Ivette , Solanich Xavier , Sánchez‐Cerrillo Ildefonso , González‐Álvaro Isidoro , Raimondo Maria Gabriella , Ramming Andreas , Martin Javier , Martínez‐Cáceres Eva , Ballestar Esteban , Vento‐Tormo1 Roser , Rodríguez‐Ubreva Javier 
Published: October 2023

AbstractIn COVID‐19, hyperinflammatory and dysregulated immune responses contribute to severity. Patients with pre‐existing autoimmune conditions can therefore be at increased risk of severe COVID‐19 and/or associated sequelae, yet SARS‐CoV‐2 infection in this group has been little studied. Here, we performed single‐cell analysis of peripheral blood mononuclear cells from patients with three major autoimmune diseases (rheumatoid arthritis, psoriasis, or multiple sclerosis) during SARS‐CoV‐2 infection. We observed compositional differences between the autoimmune disease groups coupled with altered patterns of gene expression, transcription factor activity, and cell–cell communication that substantially shape the immune response under SARS‐CoV‐2 infection. While enrichment of HLA‐DRlow CD14+ monocytes was observed in all three autoimmune disease groups, type‐I interferon signaling as well as inflammatory T cell and monocyte responses varied widely between the three groups of patients. Our results reveal disturbed immune responses to SARS‐CoV‐2 in patients with pre‐existing autoimmunity, highlighting important considerations for disease treatment and follow‐up.
Electroanalytical sensor based on CuInSe2/carbon sphere towards the non‐invasive determination of dopamine
Authors: Ren Shufang , Feng Runyan , Guo Tong , Cao Li , Lv Rui , Liu Xiaohang , Wang Qingtao , Zheng Zhixiang 
Published: October 2023

AbstractDopamine (DA) is known as a crucial neurotransmitter synthesized and secreted by dopaminergic neurons and plays a role in regulating human emotions and behaviors. The determination of DA content is of great significance in clinical application and physiological function research. An electroanalytical sensor based on the ternary semiconductor material copper indium selenide (CuInSe2) coated carbon spheres functionalized glassy carbon electrode (CuInSe2@CS/GCE) towards the non‐invasive determination of DA was constructed in this study. The performances of the sensor were characterized using cyclic voltammetry, impedance method, and differential pulse voltammetry. The results show that wrinkled CuInSe2 completely coated on CS. The sensor exhibits good electrochemical kinetics and response signal towards DA, which results from the good electrochemical activity and excellent electroconductibility of transition metal centers of the composite. The determination limit of the proposed sensor towards DA is 0.59 μM (S/N=3), and the linear measuring range is between 1–800 μM. In addition, the sensor exhibits desirable long‐term robustness, reproducibility, repetitiveness, and interference immunity. In the non‐invasive determination towards real samples of human urine and sweat, the sensor provides good recovery rate and small determination deviation.
Spatiotemporal dedifferentiation of the global brain signal topography along the adult lifespan
Authors: Ao Yujia , Yang Chengxiao , Drewes Jan , Jiang Muliang , Huang Lihui , Jing Xiujuan , Northoff Georg , Wang Yifeng 
Published: October 2023

AbstractAge‐related variations in many regions and/or networks of the human brain have been uncovered using resting‐state functional magnetic resonance imaging. However, these findings did not account for the dynamical effect the brain's global activity (global signal [GS]) causes on local characteristics, which is measured by GS topography. To address this gap, we tested GS topography including its correlation with age using a large‐scale cross‐sectional adult lifespan dataset (n = 492). Both GS topography and its variation with age showed frequency‐specific patterns, reflecting the spatiotemporal characteristics of the dynamic change of GS topography with age. A general trend toward dedifferentiation of GS topography with age was observed in both spatial (i.e., less differences of GS between different regions) and temporal (i.e., less differences of GS between different frequencies) dimensions. Further, methodological control analyses suggested that although most age‐related dedifferentiation effects remained across different preprocessing strategies, some were triggered by neuro‐vascular coupling and physiological noises. Together, these results provide the first evidence for age‐related effects on global brain activity and its topographic‐dynamic representation in terms of spatiotemporal dedifferentiation.
Morphometric properties, scaling laws and hydrologic response of the Greater Paris combined sewer system
Authors: Achour Mohamad , Chahinian Nanée , Chancibault Katia , Andrieu Hervé , Moussa Roger 
Published: October 2023

AbstractMorphometric properties of channel networks are useful tools to classify catchments and calculate their hydrological response. Scaling laws have been established for Optimal Channel Networks (OCNs), which are defined based on a generative geomorphological mechanism of minimizing the total energy dissipation. However, sewer networks obey engineering efficiency rules and are conceived based on local optimizations, both in time and space, for minimal costs. Not all the scaling laws have been verified for artificial sewer networks found in urban areas. This raises questions regarding the applicability of OCN scaling laws to sewer networks and their potential impact on the shape of the Geomorphological Instantaneous Unit Hydrograph (GIUH). Hence, this work aims to study the morphometric properties of the Greater Paris combined sewer system through a case study on twelve nested subcatchments. A two‐step methodology is used. First, the morphometric properties are analysed using the reference Horton‐Strahler, Rodríguez‐Iturbe and Moussa‐Bocquillon scaling laws. The results show that Horton‐Strahler's laws of bifurcation are verified while the length and area laws are not always verified. Rodriguez‐Iturbe and Moussa‐Bocquillon laws are verified with slightly different values of the descriptors in comparison to OCNs. Second, these morphometric properties are used to calculate four GIUHs: the reference Width Function (GWF), the Nash unit hydrograph (GN) using Horton‐Strahler ratios, the Nash Unit Hydrograph equivalent (GNe) using Moussa‐Bocquillon descriptors, and the Hayami function (GH) solution of the diffusive wave equation. We identified four catchments for which the scaling laws are verified and therefore all GIUHs are similar while for four other catchments the scaling laws are not verified and strongly impact the GIUHs. These morphometric descriptors and the GIUHs can be considered as ‘hydrological signatures’ of Combined Sewer Systems (CSSs) and are useful for the comparison and classification of hydrological responses.