bluuebunny/crossref_metadata_2025_split huggingface dataset:
The New Identity of Alginate Composite with Bentonite as a Sustainable Catalytic Natural-Based Material: Methylene Blue Decolorization in Continuous Flow-Packed-Bed Reactor
Authors: Coşkuner Filiz Bilge , Akar Yağmur , Kantürk Figen Aysel 
Published: February 2025

Abstrac
The new identity of alginate composites with bentonite as sustainable catalytic natural-based material for methylene blue (MB) decolorization in a continuous flow-packed-bed reactor was investigated. The small-scale materials were produced by drop-wise coupled freeze drying with natural organic raw ingredients, namely bentonite and alginate. The decolonization experiments were conducted by manipulating the bed depth, flow rate, and temperature systematically. The evaluation revealed that a substantial 66 mm diameter and a flow rate of 0.7 mL/min were required to attain a high removal effectiveness (99%) of continuous MB-colored dyes at a temperature of 45 °C. Alginate-based composites were very appropriate because of their facile manufacture, cost-effectiveness, biocompatibility, renewability, easy separability, absence of secondary pollutants, and ecological benignity. Estimation of the cost and environmental impact of raw material supply and processing were evaluated by using embodied energy and embodied CO2 criteria. The implementation of scale adjustments and material supply was anticipated to result in a reduction of overall expenses. Regarding the environmental impact of the material, the embodied energy values for our alginate-clay production process confirmed that freeze-drying had the highest proportion, similar to cost analysis. In summary, products based on alginate showed potential for effective decolorization in the dye industry. Further research was required to thoroughly assess the technical and commercial viability of these materials, including their unique material properties and appropriate manufacturing methods.
Regulation by Trace Amine-Associated Receptor 1 (TAAR1) of Dopaminergic-GABAergic Interaction in the Striatum: Effects of the Enhancer Drug (-)BPAP
Authors: Harsing Laszlo G. , Szénási Gábor , Fehér Balázs , Miklya Ildikó 
Published: February 2025

Abstract
Although it is well documented that the striatal GABAergic projection neurons receive excitatory and inhibitory dopaminergic innervation via D1 and D2 receptors, the trace amine-associated receptor 1 (TAAR1)-mediated regulation of this neural connection is much less studied. The presence of TAAR1 was originally detected in brain aminergic neurons, with recent evidence indicating its presence in striatal GABAergic neurons as well. The objective of the present study was to demonstrate the role of TAAR1 and signaling in dopaminergic-GABAergic interaction in the neural circuitry of the striatum. Besides trace amines, which are considered natural ligands for TAAR1, series of different exogenous drugs were identified to act on this receptor. Using the dopaminergic activity enhancer compound (-)BPAP ((-)-1-(benzofuran-2-yl)-2-propylaminopentane HCl), a potential agonist for TAAR1, we have found that it increased the electrical stimulation-induced [3H]dopamine release in rat striatal slices. This effect of (-)BPAP occurred parallel with increases of [3H]GABA release in striatum when used in 10–13–10–11 mol/L concentrations. The effects of (-)BPAP on the release of both neurotransmitters were bell-shaped. We speculated that the rising phase of the concentration-effect curves was evoked by an agonist effect of (-)BPAP on TAAR1 whereas the declining phase was a result of heterodimerization of TAAR1 with pre- and postsynaptic dopamine D2 receptors. The bell-shaped curves suggest that the (-)BPAP-induced heterodimerization of TAAR1 with dopamine D2 receptors may switch off TAAR1 signaling and switch on transduction coupled to D2 receptors. We also suggest that (-)BPAP increases synaptic strength in a hypothetical quadrilateral neuronal organization consisting of dopaminergic nerve ending, GABAergic neurons, trace amine-producing D cells, and supportive glial cell processes.
Health-related quality of life in parents of long-term childhood cancer survivors: a report from the Swiss Childhood Cancer Survivor Study - Parents
Authors: Kälin Sonja , Baenziger Julia , Mader Luzius , Harju Erika , Gumy-Pause Fabienne , Niggli Felix , Sommer Grit , Michel Gisela , Roser Katharina 
Published: February 2025

Abstract

Purpose
Having a child with cancer can profoundly impact parents’ health-related quality of life (HRQOL). However, there is a lack of knowledge about the long-term effects of childhood cancer on parents’ well-being. The current study aimed to (1) describe the HRQOL of parents of long-term childhood cancer survivors (CCS) and compare it with that of parents from the general population in Switzerland, and (2) investigate sociodemographic and cancer-related determinants of lower HRQOL in parents of CCS.

Methods
In this cross-sectional study, a total of 751 parents of CCS (mean time since diagnosis = 23.7 years, SD = 6.7 years) and 454 parents from the general population reported their HRQOL by completing the Short Form-36 (SF-36v2). Sociodemographic and cancer-related characteristics were also collected.

Results
Multilevel regression analyses showed that parents of CCS and parents from the general population had similar physical and mental HRQOL. When comparing mothers and fathers separately, there were no differences between the samples, except for higher HRQOL in the domain of physical functioning in mothers of CCS. Cancer-related characteristics were not associated with HRQOL in parents of CCS. Several sociodemographic characteristics such as being female, being from the French or Italian-speaking part of Switzerland, having a lower education, having a chronic condition, and having a migration background were associated with lower HRQOL.

Conclusion
Parents of CCS are doing well a long time after their child’s cancer diagnosis. Nevertheless, tailored support should be provided for at-risk demographic groups.
What is needed to act as a professional change agent for sustainability? A scoping review
Authors: Singer-Brodowski Mandy , Henkel Gianna-Maria , Reith Alexandra , Frank Pascal , Rieckmann Marco 
Published: February 2025

Abstract
Professionals across diverse work environments play a crucial role in facilitating and accelerating essential transformations towards sustainability. Recognising the urgent need to avert severe, irreversible harm to vital Earth systems, professional actors have been increasingly identified as a key target group for sustainability-related training programmes. While Education for Sustainable Development (ESD) scholarship has significantly contributed to identifying key competences of higher education programmes, there is a noticeable gap in research dedicated to competences needed from a professional perspective. This gap makes it challenging to design training programmes to foster such competences. To address this research gap, the authors of this article conducted a scoping review on SCOPUS, Web of Science and ERIC to identify professional sustainability competences outlined in the literature. Furthermore, they examined training activities designed to cultivate these competences and corresponding evaluation practices. By evaluating the abstracts of 475 identified articles, a sample of 37 papers was generated, which were then coded based on a qualitative content analysis. The results show that, consistent with higher education learning outcomes, systems thinking competence, interpersonal competence and strategic competence are particularly relevant. Anticipatory competence, however, assumes a secondary role, while intrapersonal competence and implementation competence – contrary to the higher education context – are considered as not emerging but pivotal. In addition, leadership competence emerges as particularly relevant in the professional field. Finally, this review indicates that intentional competence development in professional training is often not thoroughly evaluated.
Intersectional perspectives on the university belonging of international STEM students
Authors: Preuß Judith Sarah , Zimmermann Julia , Jonkmann Kathrin 
Published: February 2025

Abstract
Previous studies suggested that associations between sociodemographic characteristics and university belonging varied between subject groups. In particular, female (first-generation) students in STEM subjects were assumed to experience a “chilly climate” that was reflected in their lower levels of university belonging. But less is known about the demographic and psychological prerequisites of the university belonging of international students. In this cross-sectional study we investigated how gender, parental academic background, students’ cultural background (i.e., region of origin) and their cultural resources (i.e., study-related language skills and acculturation orientations) as well as their intersectional interplay related to the university belonging of international STEM students in Germany and compared the results to a sample of international non-STEM students. Stepwise multi-group regression analyses with cross-sectional data of N = 1,590 degree-seeking international bachelor students (n
STEM = 882, n
non-STEM = 708) at the end of their first semester in Germany did not reveal substantial differences in the level and predictors of university belonging between STEM and non-STEM international students. In both subject groups, there was no main effect of gender but some significant interactions between gender and cultural background (i.e., region of origin). First-generation students showed lower levels of university belonging than continuous-generation students. Higher levels of study-related language skills and host-culture orientation were consistently related to higher levels of university belonging but none of these variables interacted with gender. The results corroborate the need for further (intersectional) research to adequately capture the heterogeneity amongst international STEM students. Theoretical and practical implications for the implementation of tailored belonging interventions are discussed.
Antiblack Reliance on Last Resort Sanctions: A Theoretical Perspective on School Discipline
Authors: Reeves Jillian 
Published: February 2025

Abstract
This article presents a new approach to understanding higher rates of punitive discipline utilized against Black students and in Black schools by combining the theoretical construct of antiblackness with Robert Emerson’s last resort sanctions to create the framework of Antiblackness in Last Resort Sanctions. This article is the first to apply last resort sanctions to the school setting and the first to incorporate antiblackness into the concept of last resorts. This new theoretical perspective is advanced to interpret the consistent findings of disproportionate discipline of Black students across school types; additionally, this perspective can be used to guide future research on racially disparate discipline. As a framework, Antiblackness in Last Resort Sanctions identifies the ways in which the disciplinary decision-making process of educators within schools as organizations is influenced by the antiblackness that is embedded within the U.S. school system. By including analysis of both antiblackness and discipline as an organizational process, this theoretical framework contributes to existing literature on disciplinary disparities by further exploring discipline as its own setting of antiblackness rather than simply a tool of antiblackness within the education system. In doing so, this article and framework discuss how the disciplinary decision-making process reflects the manifestation of antiblackness in both the structure and purpose of disciplinary interventions. This article concludes with an exploration of how Antiblackness in Last Resort Sanctions can be utilized to inform continuing research on the use of school discipline against Black students.
Biotechnological uses of purified and characterized alkaline cellulase from extremophilic Bacillus pumilus VLC7 from Lake Van
Authors: Yilmaz Aysun Ayse , Gurkok Sumeyra 
Published: February 2025

Abstract
The present study focuses on the isolation and characterization of a bacterium from Lake Van adapted to an alkaline pH environment with significant cellulase production potential. The identified isolate, Bacillus pumilus VLC7 (GenBank Acc No: OR415888.1), exhibited the highest cellulase activity among other alkaliphilic isolates. The purification employing protein precipitation by ammonium sulfate, ultrafiltration, and ion exchange chromatography resulted in 20-fold purification with a specific activity of 16 U/mg protein. Cellulase had a molecular weight of 76 kDa, 3.13 mM Km and 0.160 U/mg Vmax values. The enzyme displayed maximum activity at pH 9.0 and 40 °C and retained at least 80% of its activity at temperatures of 25–60 °C for 90 min and pH 4–12 for one hour. Stability tests also revealed the enzyme’s resilience to various reagents, metal ions, organic solvents, and detergents. Furthermore, the biotechnological applications of cellulase were explored, demonstrating its effectiveness in fabric biopolishing (removing pilling), as well as in the removal of fabric dyes. The research findings underscore the potential of B. pumilus VLC7 as a valuable source for eco-friendly industrial biotechnology applications.

Graphical Abstract
Genomic insights into habitat adaptation of Lactobacillus species
Authors: Mejía-Caballero Alejandra , López-Sánchez Rafael , Ramos-Cerrillo Blanca , Garciarrubio Alejandro , Segovia Lorenzo 
Published: February 2025

Abstract

Lactobacillus is one of the most important genera within the lactic acid bacteria group, due to its importance in the food industry and the health field. This diversity can be explained either by their radiation in different environments or by the domestication process in artificial habitats, such as fermented foods. In this study, we performed a comparative genomic analysis of 1020 Lactobacillus genomes, categorizing them into five broad habitats: insects, vertebrates (including humans and animals), vegetables, free-living environments, and dairy products. Utilizing phylogenetic relationships, genomic distances, and gene presence/absence profiles, we identified distinct clustering patterns associated with specific environmental adaptations. Notably, species within the Lactobacillus delbrueckii clade exhibited GC content variations fivefold greater than those observed in other bacterial genera, indicating significant genomic divergence. Insect-associated species showed a strong correlation between genes for carbohydrate utilization and those for amino acid biosynthesis across all habitats. However, individual gene analyses revealed no consistent correlation between habitat adaptation and phylogenetic proximity, suggesting that Lactobacillus employs strain-specific adaptive mechanisms rather than universal genetic markers. Notably, around 50% of the genes associated with specific habitats are hypothetical. Our findings highlight the genomic complexity of Lactobacillus, driven by diverse adaptive strategies, and underscore the need for more comprehensive sampling to fully elucidate the evolutionary dynamics within this important genus.
Is it the time to reshape entrepreneurship education? State-of-the-art and further perspectives
Authors: Passarelli Mariacarmela , Bongiorno Giuseppe 
Published: February 2025

Abstract
In recent years, entrepreneurship education has attracted increasing interest among scholars, but current literature reviews often focus on specific micro or macro factors, neglecting an integrated perspective. This paper provides a comprehensive systematic literature review and bibliometric analysis of Entrepreneurship Education (EE), aiming to consolidate existing knowledge while identifying emerging trends. To address this gap, using advanced bibliometric tools, we were able to uncover key themes such as the role of human, cultural, and social capital, educational models, and the evolving function of universities in fostering entrepreneurship. Findings suggest a shift towards multidisciplinary approaches, integrating fields like psychology, ethics, and neuroscience, to enrich EE frameworks. By highlighting both established and emerging topics, this review offers actionable insights for reshaping EE to better equip future leaders with the competencies and resilience needed in an entrepreneurial society.
Phase Equilibria and Trace Element Deportments in Fe–O–Al2O3–SiO2 Slags and Molten Copper Matte at 1473 K (1200 °C), Fixed p(O2) and p(SO2), and Silica/Magnetite Saturation
Authors: Tian Miao , Klemettinen Lassi , Chen Min , Taskinen Pekka , Tiljander Mia , O’Brien Hugh , Lindberg Daniel 
Published: February 2025

Abstract
Understanding the role of major components of electric waste during smelting of these materials to recover valuable trace metals is poorly constrained by experimental data. In this study, phase equilibria and trace element deportments were studied at 1473 K (1200 °C) in conditions of combined sulfide concentrate and electric and electronic equipment waste processing in batchwise submerged lance smelting. In this system, liquid slag domain is limited by silica and magnetite saturation boundaries or primary phase fields at all alumina concentrations from alumina-free slags to silica-spinel double saturation. The impact of alumina concentration in slag at fixed oxygen and sulfur dioxide partial pressures on the distributions of Ag, Cu, In, Pb, and Sn was studied. Elemental concentrations in slag, matte, and solid magnetite (a Fe–Al spinel solid solution) were measured using electron probe microanalysis and laser ablation-ICP-mass spectrometry techniques. The observed impact of alumina on the distribution coefficient of silver, copper, and indium favored the matte phase at silica and magnetite saturation but lead and tin behaved differently within different primary phase fields as a function of alumina concentration.
Phenotyping Viral Reservoirs to Reveal HIV-1 Hiding Places
Authors: Chen Wenxuan , Berkhout Ben , Pasternak Alexander O. 
Published: February 2025

Abstract

Purpose of Review
Despite suppressive antiretroviral therapy (ART), HIV-1 reservoirs persist in various cell types and tissues and reignite active replication if therapy is stopped. Persistence of the viral reservoirs in people with HIV-1 (PWH) is the main obstacle to achieving a cure. Identification and characterization of cellular and tissue HIV-1 reservoirs is thus central to the cure research. Here, we discuss emerging insights into the phenotype of HIV-1 reservoir cells.

Recent Findings
HIV-1 persists in multiple tissues, anatomic locations, and cell types. Although contributions of different CD4 + T-cell subsets to the HIV-1 reservoir are not equal, all subsets harbor a part of the viral reservoir. A number of putative cellular markers of the HIV-1 reservoir have been proposed, such as immune checkpoint molecules, integrins, and pro-survival factors. CD32a expression was shown to be associated with a very prominent enrichment in HIV-1 DNA, although this finding has been challenged. Recent technological advances allow unbiased single-cell phenotypic analyses of cells harbouring total or intact HIV-1 proviruses.

Summary
A number of phenotypic markers have been reported by several independent studies to be enriched on HIV-1 reservoir cells. Expression of some of these markers could be mechanistically linked to the reservoir persistence, as they could for instance shield the reservoir cells from the immune recognition or promote their survival. However, so far no single phenotypic marker, or combination of markers, can effectively distinguish HIV-infected from uninfected cells or identify all reservoir cells.
Part-scale numerical simulation for laser-powder directed energy deposition: experimental calibration
Authors: de Peindray d’Ambelle L. , Sánchez Pérez M. A. , Moussaoui K. , Lachaud F. , Mabru C. , Chieragatti R. 
Published: February 2025

Abstract
To increase the environmental sustainability of products, the repair and refurbishment of damaged or obsolete components can be carried out using additive manufacturing (AM) processes. The Laser-powder directed energy deposition (LP-DED) process is an interesting candidate due to its many potential applications, particularly for the repair of metal parts. For a more thorough knowledge and understanding of the process, numerical approaches are necessary to avoid extensive and costly experimental campaigns. In this work, an original 3D thermal FEM model is proposed to predict the temperature during the LP-DED process at the macroscopic scale. The numerical simulation was based on the layer-by-layer method using an equivalent volumetric heat source and then using the superlayer method. The predicted temperature is compared with experimental temperature measurements during the deposition of stainless steel (316L) single-bead walls and multi-layer squares. The experimental results showed the influence of process parameters and deposition strategy on the temperature, which reached a maximum around 220 °C. The numerical simulation showed an overestimation of the temperature of about 120 to 200 °C compared to the experiments for all the tests carried out. A correction factor was applied to the numerical heat source which allowed to reduce the discrepancies to 50 °C. Finally, the superlayer approach led to a reduction of computational time at the expense of accuracy. Current limitations highlighted the importance of the definition of the equivalent heat source definition and boundary conditions. The chosen approach (layer-by-layer vs. superlayer) depends on the applications and the studied geometry.
Contactless in vitro detection of carboxyhemoglobin using hyperspectral imaging (HSI)
Authors: Czarkowski P. , Babian C. , Lüdtke St , Baumann S. , Dreßler J. 
Published: February 2025

Abstract
Hyperspectral imaging (HSI) allows for the contactless analysis of the composition of substances based on the reflected light and is already used in various areas of medicine. The carboxyhemoglobin (CO-Hb) concentration in blood of suspected fire victims serves to prove vitality and the cause of death. However, this metric is usually determined by spectrophotometry in the laboratory. The present study provides the basis for the future development of methods for determining CO-Hb concentrations right at the scene of a corpse or at necropsy using mobile HSI. Human erythrocyte concentrate was mixed with gaseous carbon monoxide using an aerator to produce a series of samples, which were analyzed for their CO-Hb concentration (2.9; 9.7; 18; 27.9; 39.9; 51.9; 62.3; 73.4% CO-Hb) using established spectrophotometric blood gas analysis. These blood samples were stored in a cool place at 4 °C, dripped onto a spot plate every 7 days over a period of 6 weeks, and photographed under standardized conditions (ambient lighting, distance and angle of the camera to the sample, camera settings) using the HSI camera SPECIM IQ. This device analyzes each image in the wavelength range from 400 to 1000 nm in 204 spectral bands. The data sets were used to train a lasso regression model, which provides predicted values for the CO-Hb concentration of the blood sample based on their hyperspectral properties. The results were then compared with the results of spectrophotometric measurements. The lasso regression model allowed the prediction of the CO-Hb concentration of the samples with a mean prediction error of 4.46 percentage points, independent of the sample age. Further investigations regarding pre-analytical influencing factors such as variable ambient light and tissue scattering effects, are planned to validate the robustness of the method and realize practical implementations.