bluuebunny/crossref_metadata_2025_split huggingface dataset:
A pharmacological toolkit for human microglia identifies Topoisomerase I inhibitors as immunomodulators for Alzheimer’s disease
Authors: Haage Verena , Tuddenham John F. , Comandante-Lou Natacha , Bautista Alex , Monzel Anna , Chiu Rebecca , Fujita Masashi , Garcia Frankie G. , Bhattarai Prabesh , Patel Ronak , Buonfiglioli Alice , Idiarte Juan , Herman Mathieu , Rinderspacher Alison , Mela Angeliki , Zhao Wenting , Argenziano Michael G. , Furnari Julia L. , Banu Matei A. , Landry Donald W. , Bruce Jeffrey N. , Canoll Peter , Zhang Ya , Nuriel Tal , Kizil Caghan , Sproul Andrew A. , de Witte Lotje D. , Sims Peter A. , Menon Vilas , Picard Martin , De Jager Philip L. 
Published: February 2024

AbstractWhile efforts to identify microglial subtypes have recently accelerated, the relation of transcriptomically defined states to function has been largely limited toin silicoannotations. Here, we characterize a set of pharmacological compounds that have been proposed to polarize human microglia towards two distinct states – one enriched for AD and MS genes and another characterized by increased expression of antigen presentation genes. Using different model systems including HMC3 cells, iPSC-derived microglia and cerebral organoids, we characterize the effect of these compounds in mimicking human microglial subtypesin vitro. We show that the Topoisomerase I inhibitor Camptothecin induces a CD74high/MHChighmicroglial subtype which is specialized in amyloid beta phagocytosis. Camptothecin suppressed amyloid toxicity and restored microglia back to their homeostatic state in a zebrafish amyloid model. Our work provides avenues to recapitulate human microglial subtypesin vitro, enabling functional characterization and providing a foundation for modulating human microgliain vivo.
Lytic bacteriophages interact with respiratory epithelial cells and induce the secretion of antiviral and proinflammatory cytokines
Authors: Zamora Paula F. , Reidy Thomas G. , Armbruster Catherine R. , Sun Ming , Tyne Daria Van , Turner Paul E. , Koff Jonathan L. , Bomberger Jennifer M. 
Published: February 2024

ABSTRACTPhage therapy is a therapeutic approach to treat multidrug resistant infections that employs lytic bacteriophages (phages) to eliminate bacteria. Despite the abundant evidence for its success as an antimicrobial in Eastern Europe, there is scarce data regarding its effects on the human host. Here, we aimed to understand how lytic phages interact with cells of the airway epithelium, the tissue site that is colonized by bacterial biofilms in numerous chronic respiratory disorders. We determined that interactions between phages and epithelial cells depend on specific phage properties as well as physiochemical features of the microenvironment. Although poor at internalizing phages, the airway epithelium responds to phage exposure by changing its transcriptional profile and secreting antiviral and proinflammatory cytokines that correlate with specific phage families. Overall, our findings indicate that mammalian responses to phages are heterogenous and could potentially alter the way that respiratory local defenses aid in bacterial clearance during phage therapy. Thus, besides phage receptor specificity in a particular bacterial isolate, the criteria to select lytic phages for therapy should be expanded to include mammalian cell responses.
Hypermigration of macrophages through the concerted action of GRA effectors on NF-κB/p38 signaling and host chromatin accessibility potentiatesToxoplasmadissemination
Authors: ten Hoeve Arne L. , Rodriguez Matias E. , Säflund Martin , Michel Valentine , Magimel Lucas , Ripoll Albert , Yu Tianxiong , Hakimi Mohamed-Ali , Saeij Jeroen P. J. , Ozata Deniz M. , Barragan Antonio 
Published: February 2024

AbstractMononuclear phagocytes facilitate the dissemination of the obligate intracellular parasiteToxoplasma gondii. Here, we report how a set of secreted parasite effector proteins from dense granule organelles (GRA) orchestrates dendritic cell-like chemotactic and pro-inflammatory activation of parasitized macrophages. These effects enabled efficient dissemination of the type IIT. gondiilineage, a highly prevalent genotype in humans. We identify novel functions for effectors GRA15 and GRA24 in promoting CCR7-mediated macrophage chemotaxis by acting on NF-κB and p38 MAPK signaling pathways, respectively, with contributions of GRA16/18 and counter-regulation by effector TEEGR. Further, GRA28 boosted chromatin accessibility and GRA15/24/NF-κB-dependent transcription at theCcr7gene locus in primary macrophages.In vivo, adoptively transferred macrophages infected with wild-typeT. gondiioutcompeted macrophages infected with a GRA15/24 double mutant in migrating to secondary organs in mice. The data show thatT. gondii, rather than being passively shuttled, actively promotes its dissemination by inducing a finely regulated pro-migratory state in parasitized human and murine phagocytes via co-operating polymorphic GRA effectors.ImportanceIntracellular pathogens can hijack cellular functions of infected host cells to their advantage, for example, for intracellular survival and for dissemination. However, how microbes orchestrate the hijacking of complex cellular processes, such as host cell migration, remains poorly understood. As such, the common parasiteToxoplasma gondiiactively invades immune cells of humans and other vertebrates and modifies their migratory properties. Here, we show that the concerted action of a number of secreted effector proteins from the parasite, principally GRA15 and GRA24, act on host cell signaling pathways to activate chemotaxis. Further, the protein effector GRA28 selectively acted on chromatin accessibility in the host cell nucleus to selectively boost host gene expression. The joint activities of effectors culminated in pro-migratory signaling within the infected phagocyte. We provide a molecular framework delineating howT. gondiican orchestrate a complex biological phenotype, such as the migratory activation of phagocytes to boost dissemination.
DNA density is a better indicator of a nuclear bleb than lamin B loss
Authors: Bunner Samantha , Prince Kelsey , Srikrishna Karan , Pujadas Emily Marie , McCarthy Antonia Amonu , Kuklinski Anna , Jackson Olivia , Pellegrino Pedro , Jagtap Shrushti , Eweka Imuetiyan , Lawlor Colman , Eastin Emma , Yas Griffin , Aiello Julianna , LaPointe Nathan , Schramm von Blucher Isabelle , Hardy Jillian , Chen Jason , Backman Vadim , Janssen Anne , Packard Mary , Dorfman Katherine , Almassalha Luay , Bahiru Michael Seifu , Stephens A. D. 
Published: February 2024

AbstractNuclear blebs are herniations of the nucleus that occur in diseased nuclei that cause nuclear rupture leading to cellular dysfunction. Chromatin and lamins are two of the major structural components of the nucleus that maintain its shape and function, but their relative roles in nuclear blebbing remain elusive. Lamin B is reported to be lost in blebs by qualitative data while quantitative studies reveal a spectrum of lamin B levels in nuclear blebs dependent on perturbation and cell type. Chromatin has been reported to be decreased or de-compacted in nuclear blebs, but again the data are not conclusive. To determine the composition of nuclear blebs, we compared the immunofluorescence intensity of lamin B and DNA in the main nucleus body and nuclear bleb across cell types and perturbations. Lamin B nuclear bleb levels varied drastically across MEF wild type and chromatin or lamins perturbations, HCT116 lamin B1-GFP imaging, and human disease model cells of progeria and prostate cancer. However, DNA concentration was consistently decreased to about half that of the main nucleus body across all measured conditions. Using Partial Wave Spectroscopic (PWS) microscopy to measure chromatin density in the nuclear bleb vs body we find similar results that DNA is consistently less dense in nuclear blebs. Thus, our data spanning many different cell types and perturbations supports that decreased DNA is a better marker of a nuclear bleb than lamin B levels that vary widely.
Tree Species as Biomonitors of Air Pollution around a Scrap Metal Recycling Factory in Southwest Nigeria: Implications for Greenbelt Development
Authors: Olanipon D. G. , Ayandeyi F. K. , Enochoghene A. E. , Eludoyin O. A. , Adanikin B. A. , Awotoye O. O. 
Published: February 2024

AbstractTrees are biomonitors and sinks for air pollutants but better sinking ability comes from trees with high tolerance for air pollution. Consequently, this study investigated the Air Pollution Tolerance Index (APTI) and Anticipated Performance Index (API) of six dominant tree species around a scrap metal recycling factory in Ile-Ife, Southwest Nigeria. Biochemical and physiological parameters such as the relative water content, total chlorophyll, leaf extract pH and ascorbic acid content of the leaves of the selected tree species were determined and used to compute the APTI. The biological and socio-economic characters of each tree species were equally examined to determine the API. The APTI of the selected tree species during the dry season was in the N.laevis(11.8) >A.boonei(11.2) >S. siamea(11.0) >B. micrantha(10.8)> T. orientalis(10.6)> T. grandis(9.6). According to the API grading,N.laevisandA.booneiwere classified as “good” (62.5% each) tree species for greenbelt development for both dry and wet seasons, whileT.orientaliswas also classified as a “good” (62.5% each) tree species for greenbelt development for the wet season only. Native tree species such asN.laevis,A.booneiandT.orientalisexhibited better tolerance to gaseous pollutants and are recommended for biomonitoring environmental health and greenbelt establishment.
Whole genome sequencing of a family with autosomal dominant features within the oculoauriculovertebral spectrum
Authors: Petrin AL , Machado-Paula LA , Hinkle A , Hovey L , Awotoye W , Chimenti M , Darbro B , Ribeiro-Bicudo LA , Dabdoub SM , Peter T , Breheny P , Murray J , Van Otterloo E , Rengasamy Venugopalan S , Moreno-Uribe LM 
Published: February 2024

AbstractBackgroundOculoauriculovertebral Spectrum (OAVS) encompasses abnormalities on derivatives from the first and second pharyngeal arches including macrostomia, hemifacial microsomia, micrognathia, preauricular tags, ocular and vertebral anomalies. We present genetic findings on a three-generation family affected with macrostomia, preauricular tags and uni- or bilateral ptosis following an autosomal dominant pattern.MethodsWe generated whole genome sequencing data for the proband, affected parent and unaffected paternal grandparent followed by Sanger sequencing on 23 family members for the top 10 candidate genes:KCND2, PDGFRA, CASP9, NCOA3, WNT10A, SIX1, MTF1, KDR/VEGFR2, LRRK1,andTRIM2We performed parent and sibling-based transmission disequilibrium tests and burden analysis via a penalized linear mixed model, for segregation and mutation burden respectively. Next, via bioinformatic tools we predicted protein function, mutation pathogenicity and pathway enrichment to investigate the biological relevance of mutations identified.ResultsRare missense mutations inSIX1, KDR/VEGFR2,andPDGFRAshowed the best segregation with the OAV phenotypes in this family. When considering any of the 3 OAVS phenotypes as an outcome,SIX1had the strongest associations in parent-TDTs and sib-TDTs (p=0.025, p=0.052) (unadjusted p-values). Burden analysis identifiedSIX1(RC=0.87) andPDGFRA(RC=0.98) strongly associated with OAVS severity. Using phenotype-specific outcomes, sib-TDTs identifiedSIX1with uni- or bilateral ptosis (p=0.049) and ear tags (p=0.01), andPDGFRAandKDR/VEGFR2with ear tags (both p<0.01).ConclusionSIX1,PDGFRA, andKDR/VEGFR2are strongly associated to OAVS phenotypes.SIX1has been previously associated with OAVS ear malformations and is co-expressed withEYA1during ear development. Efforts to strengthen the genotype-phenotype co-relation underlying the OAVS are key to discover etiology, family counseling and prevention.
What is the link between internal communication, organizational culture and environmental strategy? The context of company size and employee perception
Authors: Piwowar-Sulej Katarzyna , Popowicz Ewa , Sulich Adam 
Published: February 2024

PurposeThe article explores the linkages between the type of environmental strategy (ES), the use of internal communication (IC), and the greening of organizational culture (OC). Moreover, the article empirically examines whether company size matters in the use of environmental IC practices in the green context. Additionally, the article considers differences between people employed at different organizational hierarchy levels. The basis for such a comparison is their opinions about the effectiveness of communication practices.Design/methodology/approachEmpirical research employed a survey method done on 199 organizations in 2020. Statistical analyses used the chi-squared test, Kendall’s Tau-b correlation coefficient, and the Mann–Whitney U test.FindingsThe research showed that companies with a proactive green strategy more often use different communication practices related to ES and have a greener culture. The study proved that larger companies more often use the analyzed communication practices. However, we found no significant difference in opinion between middle managers and line employees about the effectiveness of these practices.Practical implicationsThe main contribution to business practice is the exploratory model based on the empirical study, which allows organizations to successfully implement the ES.Originality/valueStudies rarely combine the three organizational elements: IC, OC, and ES. This article provides new empirical evidence on relationships between features of OC, green strategy types, and communication practices.
Innovation in higher education institutions towards sustainability using LED technology
Authors: Pereira de Morais José Carlos , Neves Nelson Castro , Soveral Luís Abranches , Lima João 
Published: February 2024

Purpose
There is a growing urgent concern in involving higher education institutions (HEIs) towards an international effort in implementing a more suitable role as conductors of sustainable development. This paper/study aims to present the application of light emiting diode (LED) technology in an HEI assuming technology innovation as part of a larger institutional innovation management strategy addressing multiple dimensions in sustainability.

Design/methodology/approach
Investments in LED technology are analyzed for their impact on consumption results and quantitative comparisons between 2008 and 2022 are impaired with detailed information on the types of luminaires and the amount of installed energy.

Findings
The collected data shows a clear economic advantage of using LED technology, and the results subsidize institutional planning, considering not only ongoing technological innovation, but also educational components and community involvement in the organization sustainability strategy.

Research limitations/implications
The study is limited to a specific HEI and further comparative research should be carried out.

Practical implications
A holistic approach on sustainability objectives encourages further investment in environmental-friendly technology, example to other HEIs.

Social implications
The strategic vision of innovation is confirmed with the involvement of the community, at various levels, such as the academic community, local community, scientific international community.

Originality/value
This study addresses the lack of examples in the literature of structural planning and management systems that see sustainability as a strategy built in HEIs. The elaboration of an environmental sustainability plan places environmental sustainability at the confluence of themes such as education, investigation, use of natural resources, waste separation. For each topic are listed measures, actions, environment improvements, institution improvements and their results.
Effectiveness of oral health education on oral hygiene status among adult male inmates in central jail, Kolkata – an interventional study
Authors: Mitra Radhika 
Published: February 2024

Purpose
Oral health is an integral part of general health. Different population groups have been assessed for oral health status in India, but still, many have been neglected; one such group is the prisoners. The prison population is a unique and challenging one with many health problems, including poor oral health, which may be due to lack of knowledge about good oral health practices. This study aims to assess effectiveness of oral health education on oral health knowledge, attitude and practices and oral hygiene status among the male prison inmates of central jail in Kolkata, India.

Design/methodology/approach
An interventional study was done among 240 male convicts. The data was elicited using a structured proforma; oral health status was assessed by recording OHI-S index, and the severity of gingivitis was assessed by recording the gingival index. Oral health education was delivered by using audio-visual aid. Oral health knowledge, attitude, practices and oral hygiene and gingival status were reassessed among the inmates before and after dental education at the end of three and six months.

Findings
A significant change in oral health knowledge, attitude and practices was seen, which in turn resulted in an appreciable decrease in the mean gingival index score (1.73 ± 0.382 to 1.20 ± 0.321) and OHI-S (3.31 ± 0.815 to 2.57 ± 0.551) in all the inmates after oral health education program over the period of time.

Originality/value
The oral health education with reinforcement proved to be an effective tool to instil good oral hygiene practices in the inmates.
Non-monotonicity of risk-expected return trade-off in pricing of equity securities: the case of premium board of Nigerian Stock Exchange
Authors: Amah Peter Ngozi 
Published: February 2024

PurposeA stylized fact in finance literature is the belief in positive relationship between ex ante return and risk. Hence, a rational investor, by utility preference axiom can only consider committing fund in asset which promises commensurate higher return for higher risk. Questions have been asked as to whether this holds true across securities, sectors and markets. Empirical evidence appears less convincing, especially in developing markets. Accordingly, the author investigates the nature of reward for taking risk in the Nigerian Capital Market within the context of individual assets and markets.Design/methodology/approachThe author employed ex post design to collect weekly stock prices of firms listed on the Premium Board of Nigerian Stock Exchange for period 2014–2022 to attempt to answer research questions. Data were analyzed using a unique M Vec TGarch-in-Mean model considered to be robust in handling many assets, and hence portfolio management.FindingsThe study found that idea of risk-expected return trade-off is perhaps more general than as depicted by traditional finance literature. The regression revealed that conditional variance and covariance risks reveal minimal or no differences in sign and sizes of coefficients. However, standard errors were also found to be large suggesting somewhat inconclusive evidence of existence of defined incentive structure for taking additional risk in the market.Originality/valueIn terms of choice of methodology and outcomes, this research adds substantial value to body of knowledge. The adapted multivariate model used in this paper is a rare approach especially for management of portfolios in developing markets. Remarkably, the research found empirical evidence that positive risk-expected return trade-off, as known in mainstream literature, is not supported especially using a typical developing country data.
What are the main determinants of social expenditure? A panel data approach for EU and OECD countries
Authors: Karaalp-Orhan Hacer Simay , Evcim Nurgül , Deyneli Fatih 
Published: February 2024

PurposeThe aim of this study is to analyze which socioeconomic factors (economic, demographic, and political) most commonly affect the social expenditure of the European Union (EU) and Organization for Economic Co-operation and Development (OECD) countries.Design/methodology/approachA panel data fixed-effects model is employed for 34 OECD and 23 EU countries between 2000 and 2020.FindingsResults indicate that, in all country groups, economic factors have the most significant influence on social expenditures, with income being the primary determinant, particularly in EU countries. The negative impacts of unemployment and inflation underscore the importance of counter-cyclical measures adopted by countries to maintain stability in their social expenditures. The most influential demographic factor is found as the old-age-dependency ratio. While the rule of law affects social expenditure positively, government effectiveness and female labor force participation affect it negatively. The positive effect of Konjunkturforschungsstelle (KOF) indexes shows the globalization effect, which can be attributable to the compensation hypothesis.Practical implicationsGovernments enforce inclusive and sustainable policies to boost economic activities and GDP, thus combating inflation and unemployment and regulating the labor market and socioeconomic problems about aging populations and women’s economic participation to control social expenditures. The rule of law and institutional quality will also boost economic growth.Originality/valueThis study focuses on the effects of social expenditures in a broader view within the framework of the three main factors (economic, demographic, political) and attempts to determine the key factors that account for the differences in social expenditure between the OECD and EU countries.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-05-2023-0384
Synthesizing qualitative insights: a meta-ethnography of tourism disaster management literature
Authors: Asif Muhammad , Fazel Hesham 
Published: February 2024

Purpose
Disasters can affect the important sector of tourism across the world. This study aims to combine qualitative findings from 13 publications to enhance disaster management plans for the security and resilience of the tourist industry.

Design/methodology/approach
The authors used Noblit and Hare’s seven-step meta-ethnography method, identifying 13 qualitative studies on tourism disaster management through extensive searches in key databases (WoS, Scopus and Google Scholar), meeting inclusion criteria. NVivo 12 aided in coding, translation and comprehension of related ideas.

Findings
Five interrelated and third-ordered conceptual categories were identified: extreme natural and malevolent events, lack of risk management and emerg+ency response, sustainable tourism, tourism resilience factors, disaster awareness and preparedness. Effective strategies demand collaboration, resource allocation and local engagement.

Originality/value
This study offers a novel synthesis of qualitative research in tourism disaster management, enhancing understanding of resilience in the industry. It provides unique insights on cross-cultural dynamics, stakeholder engagement and integrated strategies while showcasing the utility of meta-ethnography in business and management research.