bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unleashing creativity in people with Parkinson’s disease: a pilot study of a co-designed creative arts therapy
Authors: Spee Blanca T. M. , de Vries Nienke M. , Zeggio Sara , Plijnaer Marjoke , Koksma Jan-Jurjen , Duits Annelien A. , Stap Thieme , Pasman Gert , Haeyen Suzanne , Darweesh Sirwan , Crone Julia , Bloem Bastiaan R. , Pelowski Matthew 
Published: January 2025

Abstract

Background
Conventional medical management, while essential, cannot address all multifaceted consequences of Parkinson’s disease (PD). This pilot study explores the potential of a co-designed creative arts therapy on health-related quality of life, well-being, and pertinent non-motor symptoms.

Methods
We conducted an exploratory pilot study with a pre-post design using validated questionnaires. Eight individuals with PD participated in the program. The investigated intervention was a 10-week creative arts therapy with weekly 90–120-min sessions, guided by three creative therapists. Participants were allowed to autonomously select from multiple creative media based on their personal preferences. Explored co-primary outcomes included health-related quality of life (PDQ-39), well-being (ICECAP-A), anxiety/depression (HADS), executive functioning (BRIEF-A), resilience/mental flexibility (FIT-60), and self-efficacy (GSES). We used paired sample t tests for pre–post analysis of the co-primary outcomes and Wilcoxon signed-rank tests for PDQ-39 sub-scores. We also included aesthetic responsiveness (AReA) and healthcare consumption (IMCQ adapted for PD) questionnaires reported as descriptive statistics.

Results
The results showed a significant reduction in anxiety and an increase in well-being. We also observed a slight improvement in cognitive functioning. Finally, we noted a reduction in healthcare consumption (fewer visits at neurologists, specialized PD nurses, and allied healthcare professionals).

Conclusion
These findings cautiously suggest that our co-designed, multi-media creative arts therapy has the potential to increase well-being and reduce anxiety, while reducing healthcare consumption. These preliminary findings support the need for a larger, randomized controlled trial to explore the therapeutic potential of creative arts therapy in PD care.
Cancer in multiple sclerosis patients following prolonged exposure to disease-modifying therapies (DMTs): a systematic review and meta-analysis
Authors: Giannopapas Vasileios , Smyrni Vassiliki , Kitsos Dimitrios K. , Stefanou Maria Ioanna , Theodorou Aikaterini , Tzartos John S. , Tsivgoulis Georgios , Giannopoulos Sotirios 
Published: January 2025

Abstract

Introduction
The current literature on the prevalence and potential association between disease-modifying therapies (DMTs) and cancer risk in the MS population has yielded mixed findings.

Methods
This study aimed to estimate cancer prevalence and cancer risk in patients with MS (PwMS) under prolonged DMT exposure. Database search include: MEDLINE PUBMED, SCOPUS, and Google Scholar.

Results
A total of 13 studies involving 333,779 PwMS were included, reporting cancer events over periods ranging from 6 to 32 years. The aggregated pooled prevalence of cancer events in MS patients receiving disease-modifying therapies (DMTs) was 3.8% (95% CI 2.6, 5.2%), with substantial heterogeneity (I
2 = 99.7%, p = 0). Two studies compared cancer events in MS patients who received DMTs versus those who did not. The relative risk of cancer associated with DMTs was 0.8 (95% CI 0.59–1.31, I
2 = 93.6%, p = 0.53), indicating no significant increase in cancer risk due to DMTs. Breast and basal cell carcinomas had a high prevalence (18.4% and 11.3, respectively) in PwMS under DMTs.

Conclusion
This study reports a 3.8% pooled prevalence of cancer in PwMS receiving DMTs. The findings of this study suggest that DMTs alone do not increase cancer risk in PwMS. Breast cancer and basal cell carcinoma had the highest prevalence among the different types of cancer.
A non-native pathogen meets a native host: Austropuccinia psidii infection reduces photosynthesis and alters non-structural carbohydrates in seedlings of Metrosideros excelsa
Authors: Nguyen Hoa , Bellingham Peter J. , Padamsee Mahajabeen , Schwendenmann Luitgard 
Published: January 2025

Abstract

Key message

Austropuccinia psidii infection and increase in diseased leaf area resulted in a reduction of photosynthesis, an upregulation of stomatal conductance, and an increase in leaf starch and sucrose content.

Abstract

Austropuccinia psidii is a biotrophic rust pathogen that causes myrtle rust, affecting over 480 species in the Myrtaceae family. The development of chlorotic and necrotic leaf areas following A. psidii infection has been shown to affect leaf gas exchange. In this study, we quantified photosynthesis, stomatal conductance, and non-structural carbohydrates in seedlings of a long-lived tree, Metrosideros excelsa (pōhutukawa), following A. psidii infection in a glasshouse experiment (infected and control seedlings) conducted over 20 weeks. The diseased leaf area rose from 8% in week 2 to 95% in week 20 after A. psidii inoculation. The photosynthetic rate declined by over 90% within 6 weeks after inoculation and was associated with biochemical damage in CO2 fixation. Stomatal conductance decreased over the first 4 weeks after inoculation and then increased. An increase in lesions and necrotic cells may inhibit stomatal regulation. Starch content was threefold higher in infected than control leaves 20 weeks after inoculation. Increased starch accumulation in the infected leaf area could be due to reduced export of newly fixed carbon from the infected leaves. Meanwhile, glucose + fructose content was 31% lower in infected leaves at the experiment’s end, likely because of leaf necrosis. If the pathogen-induced damage and loss of leaves, reduction in photosynthesis and changes in non-structural carbohydrates shown in this study also occur in wild M. excelsa seedlings and reduces their biomass, this may in turn reduce their competitive ability in the primary successions that they currently often dominate.
Phylogenetic analysis and molecular structure of NS1 proteins of porcine parvovirus 5 isolates from Mexico
Authors: Vargas-Ruiz Alejandro , Araiza-Hernández Diana Michele , González-Díaz Francisco Rodolfo , Marín-Flamand Ernesto , Sánchez Betancourt José Ivan , Sánchez-Mendoza Ana Elvia , García-Camacho Lucia Angélica 
Published: January 2025

Abstract
Porcine parvovirus 5 (PPV5) is an unclassified member of the family Parvoviridae with no reported pathogenicity, although it is associated with multisystemic, reproductive, and respiratory diseases. Its open reading frame 1 (ORF1) encodes non-structural protein 1 (NS1), which is predicted to have helicase activity that is essential for viral replication. This protein contains a C-motif with an invariant asparagine residue that forms the core of the enzyme's active site, in conjunction with the Walker A and B motifs. The aim of this study was the phylogenetic and molecular characterization of the NS1 of PPV5 through nested PCR and sequencing of three Mexican PPV5-positive samples. Subsequently, a phylogenetic tree, identity matrices of nucleotide and amino acid sequences, and a three-dimensional model of NS1 were constructed. The amplified sequences, which represented 96.9% of the PPV5 ORF1, occupied the same branch in the phylogenetic tree and exhibited the most nucleotide sequence similarity to the corresponding region of PPV4 and the most amino acid sequence similarity to the NS1 proteins of PPV4 and PPV6. A three-dimensional model of NS1 displayed a C-motif characteristic of superfamily 3 (SF3) helicases. The phylogenetic proximity of PPV5 to PPV4 and PPV6 suggests that it may belong to the genus Copiparvovirus. Further studies on helicases from viruses infecting domestic animals may be useful in developing antiviral drugs for both human and veterinary medicine.
Kollaborative Forschung und Patientenregister: Anforderungen und Perspektiven für Grundlagenforscher
Authors: Jung Georg , Lattmann Thomas , Leinweber Maria-Elisabeth , Attigah Nicolas , Hakimi Maani 
Published: January 2025

Zusammenfassung
Patientenregister sind ein zentrales Instrument der medizinischen Versorgungsforschung. Die erhobenen Daten dienen der Darstellung der Versorgungsrealität, unterstützen die Entwicklung evidenzbasierter Leitlinien und fördern die Translation von Forschungsergebnissen in die klinische Praxis.
Diese Arbeit betont die Bedeutung kollaborativer Forschungsansätze durch Patientenregister mit Fokus auf die Einhaltung komplexer regulatorischer Rahmenbedingungen.
Methodische Ansätze wie „team science“ und die Einhaltung von Qualitätsprinzipien der Dateninfrastruktur und Datenintegrität (FAIR und ALCOAplus) sind Werkzeuge zur Optimierung der Zusammenarbeit zwischen Einrichtungen verschiedener Versorgungsstufen bzw. der Qualität eines Patientenregisters.
Die Einhaltung ethischer, rechtlicher und regulatorischer Anforderungen wie der DSGVO und medizinischer Leitlinien zum Betrieb von Patientenregistern ist für die erfolgreiche Auswertung solcher Registerdaten unerlässlich.
Diese Arbeit soll zeigen, dass die Translation in diverse Bereiche der akademischen Medizin möglich ist und durch die Nutzung von Registerdaten erleichtert werden kann. Patientenregister schlagen eine Brücke zwischen Versorgungsrealität und Forschung und tragen darüber hinaus zur Verbesserung der medizinischen Ausbildung bei.
Co-culture of Helicobacter pylori with oral microorganisms in human saliva
Authors: Scholz Konstantin Johannes , Höhne Annabelle , Wittmer Annette , Häcker Georg , Hellwig Elmar , Cieplik Fabian , Waidner Barbara , Al-Ahmad Ali 
Published: January 2025

Abstract

Objective

Helicobacter pylori is known for colonizing the gastric mucosa and instigating severe upper gastrointestinal diseases such as gastritis, gastroduodenal ulcers, and gastric cancer. To date, there is no data available on the oral cavity as transmission site, whether H. pylori can survive in the oral cavity or in human saliva. The aim of the study was to investigate the influence of oral microorganisms and human saliva on the survival of H. pylori in human saliva.

Methods

H. pylori strains KE, a motile derivate of type strain H. pylori 26695, and H. pylori SS1, a clinical isolate from a gastric biopsy, were grown in human pooled saliva (pooled from 4 healthy human donors, 0.22 μm filter-sterilized) or in BBF (Brucella browth formula; control) either as mono-cultures or in co-culture with Streptococcus mutans, Streptococcus oralis, Actinomyces naeslundii, Lacticaseibacillus casei and Candida dubliniensis. Bacterial survival of H. pylori and the oral microorganisms were investigated using colony forming units (CFU) assay and MALDI-TOF MS at baseline and after 24, 48 and 168 h.

Results
In saliva, H. pylori KE demonstrated enhanced survival in co-culture with S. mutans, A. naeslundii, and C. dubliniensis, enduring for at least 48 h. In contrast, L. casei and S. oralis inhibited H. pylori KE in saliva. H. pylori KE could not be cultured after 168 h in saliva, neither in mono- nor co-culture. In contrast, H. pylori SS1 in saliva could be cultured after 168 h in co-culture with S. mutans and C. dubliniensis, but not in mono-culture. In BBF, H. pylori KE could be cultured after 168 h with S. mutans, L. casei and C. dubliniensis, and H. pylori SS1 with L. casei and C. dubliniensis, but not with S. mutans. Notably, the co-cultured microorganisms survived at high CFU numbers similar to those of the monocultures.

Conclusion
The study suggests that H. pylori can transiently survive in human saliva and even with presence of certain oral microorganisms. However, it may not be a permanent resident of the oral microbiota. The co-survival with oral microorganisms emphasizes the necessity for studying the role of the oral microbiota in the infectious and transmission cycle of H. pylori.
Reconstructing the long-term ecological history of Long Island, Furneaux Group (Bass Strait), Lutruwita/Tasmania
Authors: Bessell-Koprek Madeleine , Haberle Simon Graeme , Ondei Stefania , Harris Stephen , Bowman David M. J. S. 
Published: January 2025

Abstract
The Bass Strait islands are the elevated remnants of a now-submerged continental land bridge that connected the mainland of Australia to Lutruwita/Tasmania during low sea level phases of the Quaternery period. The Furneaux Group is made up of around 100 islands, harbouring a rich diversity of plants derived from glacial refugia and stepping-stone dispersal as millennial-scale climate change altered the land and seascape configuration of the land bridge. Despite the region’s significance, long-term ecological and environmental dynamics of most of these islands remain poorly known. We present the first palaeoecological study of Long Island, a small granite island of the Furneaux group, currently covered by extensive grasslands in the west and patches of forest and woodland in the east and north of the island. We use decadal to centennial-scale resolution palaeoecological evidence for vegetation, animal and fire dynamics, alongside historical accounts of vegetation changes inferred from aerial photo analysis, to develop a comprehensive record of the island’s long-term ecology. Results show that grasslands have been an important feature of the island for at least the last 1000 years, and are reminiscent of Last Glacial Maximum grasslands of the now-submerged Bassian Plain. Both aerial photo analysis and the palaeoecological record show increases in forest cover on the island’s eastern corner over the past four decades. We discuss these ecological dynamics in the context of environmental and climatic shifts. This study emphasises the importance of palaeoecological studies, specifically their value in understanding modern ecosystems in their historic context. This data is critical in understanding the island’s current landscape and how this might change into the future.
Margin matters: analyzing the impact of circumferential margin involvement on survival and recurrence after incomplete total mesorectal excision for rectal cancer
Authors: Alipouriani A. , Almadi F. , Rosen D. R. , Liska D. , Kanters A. E. , Ban K. , Gorgun E. , Steele S. R. 
Published: January 2025

Abstract

Background
Incomplete mesorectal excision during rectal cancer surgery often leads to positive circumferential margins, with uncertain prognostic impacts. This study examines whether negative margins can mitigate the poorer prognosis typically associated with incomplete total mesorectal excision (TME) in rectal cancer surgery, thus potentially challenging the prevailing emphasis on complete mesorectal excision.

Patients and methods
A retrospective analysis was conducted on patients who underwent proctectomy for rectal adenocarcinoma with incomplete TME at a single center from 2010 to 2022. Patients were stratified by margin status as determined by pathologic analysis into three groups: involved, not involved with closest margin distance ≤ 2 mm, and not involved with closest margin distance > 2 mm. Outcomes included recurrence and survival. Effects of neoadjuvant therapy protocols on margin status were also assessed.

Results
From 2010 to 2022, 7941 patients underwent proctectomy for rectal cancer, with 236 (3%) having incomplete TME. The median age of these patients was 64 years, and 63% were male. Overall, margin involvement was observed in 54 (23%) patients. The median tumor size was 3.05 cm (interquartile range (IQR): 2–6) for the whole group. Involved margins (23.2%) had reduced overall survival (60.5 months versus 87.3 months, p < 0.001), increased local recurrence (20.4% versus 9.4%, p = 0.024), and lower disease-free survival (45.2 versus 58.9 months, p = 0.006) versus uninvolved margins. Margin involvement was prognostic for decreased survival even after adjusting for confounders (p < 0.05). Among uninvolved margins, distance (> 2 mm versus ≤ 2 mm) did not affect outcomes. Total neoadjuvant therapy (versus standard chemoradiation) was associated with lower involved margins (p = 0.007).

Conclusions
Positive margins retain negative prognostic impact with incomplete TME. Optimization of surgical resection remains vital. Total neoadjuvant therapy was associated with a lower rate of margin involvement.
CT angiography of acute aortic syndrome in patients with chronic kidney disease
Authors: Papachristodoulou Angeliki , Ghibes Patrick , Pentara Natalia Valeria , Alexandratou Maria , Levitin Abraham , Gadani Sameer , Partovi Sasan , Psoma Elizabeth , Rafailidis Vasileios , Prassopoulos Panos 
Published: January 2025

Abstract
The term acute aortic syndrome (AAS) refers to a range of different entities, including dissection, intramural haematoma and penetrating atherosclerotic ulcer. Patients with chronic renal disease and particularly those with dominant polycystic kidney disease are susceptible to this pathology, given the underlying renal arteriopathy and hypertension. Imaging plays a crucial role in diagnosing, grading and guiding management of these patients, with computed tomography angiography (CTA) being on the frontline. Albeit of overlapping of imaging findings between these conditions, specific imaging characteristics help discriminate and guide treatment. Given the nephrotoxic contrast agent involved, tailored CTA protocols or alternative imaging modalities such as MRI or US are necessary in this patient population. This review article discusses the main imaging findings of entities found in the spectrum of AAS, as well as the appropriate use and protocol of imaging modalities, focusing on the appropriate use of nephrotoxic contrast agents, the preservation of renal function and maintenance of optimal diagnostic accuracy.
Polynomial Chaos Expansion: Efficient Evaluation and Estimation of Computational Models
Authors: Fehrle Daniel , Heiberger Christopher , Huber Johannes 
Published: January 2025

Abstract
We apply Polynomial chaos expansion (PCE) to surrogate time-consuming repeated model evaluations for different parameter values. PCE represents a random variable, the quantity of interest (QoI), as a series expansion of other random variables, the inputs. Repeated evaluations become inexpensive by treating uncertain parameters of a model as inputs, and an element of a model’s solution, e.g., the policy function, second moments, or the posterior kernel as the QoI. We introduce the theory of PCE and apply it to the standard real business cycle model as an illustrative example. We analyze the convergence behavior of PCE for different QoIs and its efficiency when used for estimation. The results are promising both for local and global solution methods.
Large Deformation Behavior of Plane Periodic Truss Networks. Part 1. Closed-Form Solution for Single Node Cells
Authors: Cuomo Massimo , Boutin Claude , Pannitteri Carmelo 
Published: January 2025

Abstract
This article focuses on the derivation of explicit descriptions of networks in large deformation through the homogenization method of discrete media. Analytical models are established for the in-plane behavior of a planar periodic truss, whose cell contains a single node, as frequently encountered in practice. The cell is composed of bars that support only axial forces and are connected by perfect hinges. For the considered type of trusses, (given that the equilibrium conditions of the node and of the cell coincide) closed-form expressions for the local behaviour in the case of large deformations can be derived. This case makes it possible to combine the non-linearities arising from large deformations on the one hand and rheological characteristics on the other, and to compare their respective effects as a function of cell morphology. The results are illustrated by the shear and extension responses of specific trusses. The analysis is carried out for bars with stiffening, linear or softening behavior. The combination of the effects of geometrical non-linearities, rheological non-linearities and anisotropy results in particularly rich behaviors of the network.
Heterogeneous fracture toughness of human cortical bone tissue
Authors: Levy Maxime , Yosibash Zohar 
Published: January 2025

Abstract
CT-based finite element analysis (FEA) of human bones helps estimate fracture risk in clinical practice by linking bone ash density (

$$\rho _{ash}$$

ρ

ash

) to mechanical parameters. However, phase field models for fracture prediction require the heterogeneous fracture toughness

$$G_{Ic}$$

G

Ic

, which can be derived from the critical stress intensity factor

$$K_{Ic}$$

K

Ic

, determined through various experimental methods. Due to a lack of standards for determining cortical bone’s

$$K_{Ic}$$

K

Ic

, an experimental campaign is presented using 53 cortical specimens from two fresh frozen femurs to investigate whether a correlation exists between

$$K_{Ic}$$

K

Ic

and

$$\rho _{ash}$$

ρ

ash

. We investigated various experimental techniques for correlating

$$K_{Ic}$$

K

Ic

with

$$\rho _{ash}$$

ρ

ash

. We conducted FEAs employing the phase field method (PFM) to determine the most suitable correlation among the five possible ones stemming from the experimental methods. The ASTM standard using displacement at force application point was found to be the recommended experimental method for the estimation of

$$K_{Ic}$$

K

Ic

perpendicular to osteons’ direction

$$\begin{aligned} K_{Ic} [MPa\sqrt{m}]{=}1.89\left( \rho _{ash} [gr/cc] \right) ^{1.88} \,\, R^2{=}0.5374. \end{aligned}$$

K

Ic

[
M
P
a

m

]

=
1.89

ρ

ash

[
g
r
/
c
c
]

1.88

R
2

=
0.5374
.

The corresponding statistical critical energy release rate bounds were determined:

$$\begin{aligned} G_{Ic}[N/m]= 321.94 (\rho _{ash}[gr/cc])^{1.69} \times exp(\pm 2SD), \end{aligned}$$

G

Ic

[
N
/
m
]

=
321.94

(

ρ

ash

[
g
r
/
c
c
]

)

1.69

×
e
x
p

(
±
2
S
D
)

,

with a standard deviation

$$SD= 0.30$$

S
D
=
0.30

representing a 95.4% confidence interval. The average

$$G_{Ic}$$

G

Ic

resulted in good correlations between the predicted fracture force by PFM-FEA of four representative specimens and experimental fracture forces. The proposed correlations will be used in CT-based PFM FEA to estimate the risk of hip and humeral fractures.
Prenatal diagnosis following preimplantation genetic testing for monogenic conditions: a single centre record linkage study
Authors: Poulton Alice , Menezes Melody , Hardy Tristan , Lewis Sharon , Hui Lisa 
Published: January 2025

Purpose
Professional bodies currently advise all pregnant individuals undertake confirmatory prenatal diagnostic testing following preimplantation genetic testing for monogenic conditions (PGT-M). We aimed to ascertain the uptake of prenatal diagnostic testing following PGT-M in a large single-centre population.

Methods
This observational linkage study was undertaken using routinely collected outcome data from PGT-M cycles performed at one of Australia’s largest PGT-M providers and a statewide dataset of all prenatal samples undergoing cytogenetic analysis in Victoria, Australia, between 2015 and 2022.

Results
During the study period, there were 176 clinical pregnancies following the transfer of a PGT-M-tested embryo in 132 patients. Eleven patients undertook confirmatory prenatal diagnostic testing in 12 pregnancies, representing a confirmatory testing rate of 8.3% [95% CI: 4.7–14.3%] per patient and 6.8% [95% CI: 3.9–11.5%] per pregnancy. The 176 clinical pregnancies resulted in 154 (87.5%) live births and pregnancies ongoing at the time of reporting, 21 (11.9%) pregnancy losses ≤ 20 weeks gestation, and 1 (0.6%) stillbirth.

Conclusions
Most patients who conceive following the transfer of a PGT-M-tested embryo do not undertake confirmatory prenatal diagnostic testing. The low uptake of confirmatory testing raises important considerations for genetic counselling for PGT-M and the acceptability of current clinical practice recommendations.