bluuebunny/crossref_metadata_2025_split huggingface dataset:
Daytime DNase-I Administration Protects Mice From Ischemic Stroke Without Inducing Bleeding or tPA-Induced Hemorrhagic Transformation, Even With Aspirin Pretreatment
Authors: Di Gaohong , Vázquez-Reyes Sandra , Díaz Blanca , Peña-Martinez Carolina , García-Culebras Alicia , Cuartero María I. , Moraga Ana , Pradillo Jesús M. , Esposito Elga , Lo Eng H. , Moro María A. , Lizasoain Ignacio 
Published: January 2025

BACKGROUND:
Acute ischemic stroke treatment typically involves tissue-type plasminogen activator (tPA) or tenecteplase, but about 50% of patients do not achieve successful reperfusion. The causes of tPA resistance, influenced by thrombus composition and timing, are not fully clear. Neutrophil extracellular traps (NETs), associated with poor outcomes and reperfusion resistance, contribute to thrombosis. DNase-I, which degrades neutrophil extracellular traps, could improve thrombolytic efficacy. However, more studies are needed to understand the impact of DNase-I in tPA-sensitive stroke models, the safety of coadministering DNase-I and tPA regarding hemorrhagic transformation (HT), optimal timing for use, and effects on aspirin-treated animals.

METHODS:
We used in situ thromboembolic stroke, a tPA-sensitive model, where late tPA administration causes HT. Middle cerebral artery occlusion was induced at different zeitgeber times (ZT) to study the optimal timing for administration. DNase-I, tPA, and aspirin were administered at various times to evaluate their effects.

RESULTS:
DNase-I reduced infarct volume and improved functional outcomes 24 hours post-middle cerebral artery occlusion by decreasing plasma and cortical neutrophil extracellular trap levels. DNase-I caused no bleeding or impact on HT induced by late tPA. Its protective effect was only seen when given during the daytime (rodent inactive phase; ZT4–7), not overnight (active phase; ZT13–16). Chronic aspirin pretreatment increased tPA-induced HT but did not change the protective effects of DNase-I, with or without tPA.

CONCLUSIONS:
Our study demonstrates that daytime (inactive phase) DNase-I administration is a safe and effective treatment for experimental stroke. This is particularly important given the 2 ongoing clinical trials for stroke patients.

REGISTRATION:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT05203224 and NCT05880524.
Neural Correlates of the Musicianship Advantage to the Cocktail Party Effect
Authors: Ostrand Avery E. , Johnson Vinith , Gazzaley Adam , Zanto Theodore P. 
Published: January 2025

Abstract
Prior research has indicated musicians show an auditory processing advantage in phonemic processing of language. The aim of the current study was to elucidate when in the auditory cortical processing stream this advantage emerges in a cocktail-party-like environment. Participants (n = 34) were aged 18–35 years and deemed to be either a musician (10+-year experience) or nonmusician (no formal training). EEG data were collected while participants were engaged in a phoneme discrimination task. During the task, participants were asked to discern auditory “ba” and “pa” phonemes in two conditions: one with competing speech (target with distractor [TD]) and one without competing speech (target only). Behavioral results showed that musicians discriminated phonemes better under the TD condition than nonmusicians, whereas no performance differences were observed during the target only condition. Analysis of the EEG ERP showed musicianship-based differences at both early (N1) and late (P3) processing stages during the TD condition. Specifically, musicians exhibited decreased neural activity during the N1 and increased neural activity during the P3. Source localization of the P3 showed that musicians increased activity in the right superior/middle temporal gyrus. Results from this study indicate that musicians have a phonemic processing advantage specifically when presented in the context of distraction, which arises from a shift in neural activity from early (N1) to late (P3) stages of cortical phonemic processing.
Between Science and Architecture: Exhibiting Science and Technology in Interwar Europe
Authors: Surman Jan 
Published: January 2025

Abstract
Museums and exhibitions of science and technology have received considerable attention in recent historiography. However, little has been done to look beyond individual localities and national borders. Using Yehuda Elkana's concept of “images of knowledge,” this article shows how a comparison of four interwar projects located across Europe - in Czechoslovakia, Germany, the Soviet Union, and Switzerland - helps to highlight commonalities in the understanding of science at the time. Although these exhibition projects were located in different political systems and their proponents superficially presented irreconcilable ideas about science, their ideas about progress or the need to overcome national and disciplinary boundaries show a preponderance of similarities. However, the political-ideological divide is visible in the way they conceive of the relationship between science and society. Finally, by looking at how images of knowledge were co-produced at the interface of science, architecture, and politics, this article argues for a broadening of the source base to include non-scientific and non-human actors.
Evaluation and Statistical Correction of Area-Based Heat Index Forecasts That Drive a Heatwave Warning Service
Authors: Loveday Nicholas , Carroll Maree 
Published: January 2025

Abstract
This study evaluates the performance of the area-based, district heatwave forecasts that drive the Australian heatwave warning service. The analysis involves using a recently developed approach of scoring multicategorical forecasts using the fixed risk multicategorical (FIRM) scoring framework. Additionally, we quantify the stability of the district forecasts between forecast updates. Notably, at longer lead times, a discernible overforecast bias exists that leads to issuing severe and extreme heatwave district forecasts too frequently. Consequently, at shorter lead times, forecast heatwave categories are frequently downgraded with subsequent revisions. To address these issues, we demonstrate how isotonic regression can be used to conditionally bias correct the district forecasts. Finally, using synthetic experiments, we illustrate that even if an area warning is derived from a perfectly calibrated gridded forecast, the area warning will be biased in most situations. We show how these biases can also be corrected using isotonic regression which could lead to a better warning service. Importantly, the evaluation and bias correction approaches demonstrated in this paper are relevant to forecast parameters other than heat indices.

Significance Statement
Heatwaves have terrible impacts on society. Understanding the performance of heatwave forecasts and warnings is important so that we can understand how they can be improved to reduce negative health impacts. We demonstrate techniques to evaluate the performance, biases, and stability of area-based forecasts that are used by a warning service. We illustrate how area-based forecasts can be improved through conditional bias correction. Additionally, we conduct a synthetic experiment that shows that deriving an area-based warning from a perfectly calibrated gridded forecast may lead to biased warnings.
A Comparison of Wound Related Complications Between Intramedullary and Lateral Plate Osteosynthesis After Fibula Osteotomy in the Lateral Approach Total Ankle Replacement
Authors: Phegan Michael , Wines Andrew 
Published: January 2025

Background: Total ankle replacement (TAR) has evolved in the last decade from a procedure rife with complication and failure to a promising alternative to arthrodesis. The ability to maintain ankle joint range of motion is showing great promise in patient-reported outcomes, postsurgical pain, as well as long-term sequalae of joint fusion. Although TAR can be performed via either an anterior or lateral approach both with their own sets of benefits and potential complications, the consensus seems to be that one is no better than the other when performed by high-volume surgeons. Methods: Data were gathered over a 6-year period looking at an age- and gender-matched cohort of lateral approach TARs with either plate osteosynthesis or fibula nail fixation in the management of the fibula osteotomy and compared reoperation and infection rates with the working hypothesis that nail fixation would afford better outcomes. Secondary outcomes including rates of fibula union, tourniquet time as an adjunct to procedure length, and patient-reported outcome measures (PROMs) in the form of FAOS Quality scores were included. Results: A total of 234 TARs were analyzed. Forty-five of these used intramedullary nail fixation (IM), and a control group of 45 plate osteosynthesis (PO) fixation cases was correlated. The Foot and Ankle International guidelines on reporting for complications arising from total ankle arthroplasty that resulted in reoperation were applied to the data set, and it was noted that 13 of 19 reoperations were noted in the PO group, which was statistically significant. Also of note, 12 of 16 patients treated with antibiotics, which included all those cases within the reoperation group, were noted to come from the PO group. This was also of statistical significance. Interestingly, the rate of fibula nonunion in the PO group was lower (8.88%) than that of the IM group (23.25%). Conclusion: Our results confirmed that the rates of reoperation and infection were statistically significantly improved with the use of nail fixation over plate fixation. This was tempered by a concerningly high nonunion rate. This article shows promising results for the use of a simple technique in improving long-term outcomes in ankle arthroplasty.
Exploring reverse silicate weathering across geological time: a review
Authors: Baldermann Andre , Banerjee Santanu , Löhr Stefan C. , Rudmin Maxim , Warr Laurence N. , Chakraborty Arpita 
Published: January 2025

Abstract
Marine clay mineral authigenesis, referred to as reverse (silicate) weathering, is one of the first-order controls on seawater pH through the generation of acidity and thus plays a significant role in controlling carbon cycling between marine sediments, oceans and the atmosphere over geological timescales. Reverse weathering is mainly regulated by the rates of silicate and carbonate weathering on the continents, the reactivity of detritus supplied to the oceans and the rates of seafloor weathering. These processes provide essential dissolved components (e.g. K+, Mg2+, Ca2+, Si(OH)4, Al3+, Fe2+/3+) to the marine porewater inventory that cause authigenic clay minerals, such as odinite, glauconite, celadonite and greenalite, to form close to the sediment–seawater interface. Such clay mineral reactions impact the sedimentary cycling versus sequestration of chemical elements, importantly Si, Fe, Mg and K, and consequently contribute to the fluctuations in climate and seawater composition recorded in marine archives over geological time. This review explores the links between reverse silicate weathering and the climate system across geological timescales and provides estimates of the elemental uptake fluxes associated with modern-day clay mineral authigenesis. Novel isotope proxies (e.g. δ41K and δ30Si) and promising new dating techniques (e.g. in situ Rb/Sr geochronology) provide improved constraints on the timing, kinetics and environmental significance of clay mineral reactions on the ocean floor. We also consider recent geoengineering developments linked to reverse weathering reactions, such as ongoing attempts to reduce atmospheric CO2 concentrations via marine alkalinity enhancement and the application of marine clay mineral-based slow-release fertilizers to soils to optimize nutrient availability.
Monocytes serve as Shiga toxin carriers during the development of hemolytic uremic syndrome
Authors: Sun Xinlei , Qu Shuang , Zhou Fenglian , Shi Fujie , Wu Yunfei , Gu Lin , Liu Minghui , Bian Zhen , Shi Lei , Liu Zhihong , Liu Yuan , Zen Ke 
Published: January 2025

Abstract
Shiga toxin (Stx)-induced hemolytic uremic syndrome (HUS) poses a life-threatening complication for which a definitive treatment remains elusive. To exert its cytotoxic effect on renal cells, Stx must be delivered from the infected intestines to the kidney. However, the mechanism underlying Stx delivery remains unclear. Here we pinpoint monocytes as the primary carriers responsible for transporting Stx2 to the renal region. Through single-cell sequencing analysis of Stx2-B-bound peripheral white blood cells sorted by flow cytometry, we observe that nearly all monocytes exhibit strong Stx2-B binding, whereas less than 10% of neutrophils are associated with Stx2-B, albeit with a lower affinity. Further examination of the single-cell dataset and cell binding assays suggest that monocytes likely bind to Stx2-B through the Toll-like receptor 4. Remarkably, Stx-laden monocytes demonstrate their ability to transport Stx2 to human renal glomerular endothelial cells (HRGEC), subsequently inducing apoptosis in HRGEC. In a mouse model of Stx1/2-positive EDL933 infection-induced HUS, the presence of Stx2-positive monocytes in peripheral blood and infiltrated kidney tissues was observed. Finally, depleting monocytes through the usage of a CD14 neutralizing antibody or blocking monocyte chemotaxis via inhibition of CCL2 notably mitigates kidney injury and dysfunction caused by lipopolysaccharide (LPS)/Stx2 treatment. Our findings unveil the pivotal role of monocytes in Stx delivery during STEC infection and offer a promising therapeutic approach for Stx-induced HUS.
Cu-Ag/SBA-15 nano catalysts for the control of microorganisms in water
Authors: Ganji Saidulu , Kola Ramesh , Gullapelli Kumaraswamy , Martha Ramesh 
Published: January 2025

Abstract
Because of their uniform and regular channels, adjustable pore size, large surface area, controllable wall composition, high hydrothermal stability, ease of functional modification, and good accessibility of larger reactant molecules, mesoporous siliceous SBA-15 is of excellent catalyst carrier that is highly versatile and has been used extensively to prepare a variety of supported catalysts with ideal catalytic properties. In this study, we report the synthesis, characterization, and catalytic application of Cu-Ag/ SBA-15 nanoalloy catalysts towards the control of microorganisms in drinking water has been reported. The Cu-Ag/SBA-15 nanoalloy catalysts with different molar mass ratio of copper to silver (Cu:Ag = 1: 0, 0.75: 0.25, 0.5: 0.5, 0.25: 0.75, 0: 1) keeping 1weight % total loading of copper and silver metals on SBA-15 support have been prepared by incipient wetness impregnation method and characterized by various characterization techniques like, low angle XRD, wide angle XRD, N2-physcisorption and scanning electron microscopy techniques. The anti-bacterial activity of the catalysts was measured qualitatively by testing the presence of coliforms in water after contacting with the catalyst at room temperature. These nanoalloy catalysts found to be effective in controlling the microorganisms in drinking water. Among the series of the catalysts prepared, 0.25Cu-0.75Ag /SBA-15 catalyst showed superior catalytic activity. The high catalytic performance of the catalyst is due to its high surface area.
A resource of longitudinal RNA-seq data of Holstein cow rumen, duodenum, and colon epithelial cells during the lactation cycle
Authors: Gao Yahui , Liu George E. , Ma Li , Li Cong-jun , Baldwin Ransom L 
Published: January 2025

Abstract

Objective
As one of the most important ruminant breeds, Holstein cattle supply a significant portion of milk and dairy for human consumption, playing a crucial role in agribusiness. The goal of our study was to examine the molecular adaptation of gastrointestinal tissues that facilitate milk synthesis in dairy cattle.

Data description
We performed RNA-seq analysis on epithelial cells from the rumen, duodenum, and colon at eight different time points: Days 3, 14, 28, 45, 120, 220, and 305 in milk, as well as the dry period. Samples were taken from five multiparous dairy cows as biological replicates per tissue per stage, except for Days 14 and 28, for which the sample size was three. These tissues each serve critical and distinct roles in the digestion and absorption of nutrients and are all vital for providing the necessary substrates required for milk production. Understanding the intricate connections between the tissues involved in providing nutrients necessary to support milk synthesis and their role in digestion can deepen the understanding of lactation physiology. This resource aims to deliver in-depth insights into cattle lactation, highlighting the distinct traits of gastrointestinal tissues and illuminating the intricate transcriptomic dynamics throughout the lactation period.
Time and cost of linking administrative datasets for outcomes assessment in a follow-up study of participants from two randomised trials
Authors: Shahbaz Mohammad , Harding Jane E. , Milne Barry , Walters Anthony , Underwood Lisa , von Randow Martin , Jacob Lena , Gamble Greg D. ,   for the ANCHOR Study Group, Crowther Caroline A , Dalziel Stuart R , Eagleton Carl L , McKinlay Chris JD 
Published: January 2025

Abstract

Background
For the follow-up of participants in randomised trials, data linkage is thought a more cost-efficient method for assessing outcomes. However, researchers often encounter technical and budgetary challenges. Data requests often require a significant amount of information from researchers, and can take several years to process. This study aimed to determine the feasibility, direct costs and the total time required to access administrative datasets for assessment of outcomes in a follow-up study of two randomised trials.

Methods
We applied to access administrative datasets from New Zealand government agencies. All actions of study team members, along with their corresponding dates, were recorded prospectively for accessing data from each agency. Team members estimated the average time they spent on each action, and invoices from agencies were recorded. Additionally, we compared the estimated costs and time required for data linkage with those for obtaining self-reported questionnaires and conducting in-person assessments.

Results
Eight agencies were approached to supply data, of which seven gave approval. The time from first enquiry to receiving an initial dataset ranged from 96 to 854 days. For 859 participants, the estimated time required to obtain outcome data from agencies was 1,530 min; to obtain completed self-reported questionnaires was 11,025 min; and to complete in-person assessments was 77,310 min. The estimated total costs were 20,827 NZD for data linkage, 11,735 NZD for self-reported questionnaires, and 116,085 NZD for in-person assessments. Using this data, we estimate that for a cohort of 100 participants, the costs would be similar for data linkage and in-person assessments. For a cohort of 5,000 participants, we estimate that costs would be similar for data linkage and questionnaires, but ten-fold higher for in-person assessments.

Conclusions
Obtaining administrative datasets demands a substantial amount of time and effort. However, data linkage is a feasible method for outcome ascertainment in follow-up studies in New Zealand. For large cohorts, data linkage is likely to be less costly, whereas for small cohorts, in-person assessment has similar costs but is likely to be faster and allows direct assessment of outcomes.
SQUARE-IT: a proposed approach to square the identified research problem in the literature with the objectives, the appropriate clinical research question, and the research hypothesis
Authors: Alfuth Martin , Klemp Jonas , Schmidt Annette , Streese Lukas , Ramadanov Nikolai , Prill Robert 
Published: January 2025

Abstract
The purpose of this article is to design and introduce the SQUARE-IT approach to help scientists and clinicians in research to align important research problems with the objectives, the appropriate clinical research questions to be answered, and the research hypotheses to be investigated in medical and therapeutic specialties. Research ideas can be generated primarily through simple methods such as brainstorming and mind mapping. However, transforming ideas into a valid research question is not as easy as it may seem, as the mere presence of an idea does not guarantee that the researcher has already uncovered existing knowledge on a particular topic or identified the actual research problem. Therefore, the SQUARE-IT items are developed, described, and critically discussed with reference to the scientific literature. They ask whether the identified research problem is ‘Specific’, ‘Quantifiable’, ‘Usable’, ‘Accurate’, ‘Restricted’, ‘Eligible’, ‘Investigable’, and ‘Timely’. Before formulating the focused clinical question, SQUARE-IT can be used as a preparatory step to enable researchers to organize the relevant information that has been explored to date and to assess whether additional information is needed, thereby identifying current research gaps. In addition, it should facilitate the effectiveness and efficiency of evidence-based practice to ensure high quality patient care. Using SQUARE-IT as a framework, further elaboration of the approach and addition of other aspects are warranted to advance the discussion and improve methods of evidence-based practice in medical and therapeutic specialties for quality improvement of patient care.
Validation of preoperative predictor score for difficult laparascopic cholecystectomy and a modified intraoperative grading score of the difficulty of laparascopic cholecystectomy: from a resource limited setting
Authors: Ahmed Nurhussen Mossa , Djote Surafel Mulatu , Alemayehu Getachew Desta , Amtataw Wondwossen , Ahmed Sitotaw Mossa 
Published: January 2025

Abstract

Background
Difficult laparascopic cholecystectomy has greater risk of biliary, vascular and visceral injuries. A tool to predict the difficulty help to prepare a head and avoid complications.

Aim
the aim of this study is validation of preoperative predictor score and a modified intraoperative grading score for difficulty of laparascopic cholecystectomy.

Methods
This study was a cross sectional, hospital based study on 200 patients. There are total of 10 scores for preoperative predictor score and 16 scores for the modified intraoperative grading of LC. Structured checklist questionnaire was used.

Result
prevalence of difficult LC was 40%. age greater than or equal to 50years, history of admission for acute cholecystitis, BMI > 30, palpable GB, impacted stone on imaging, adhesion burying GB, time to identify cystic artery/duct, bile/stone spillage and type of ligature were statistically significantly factors for difficult laparascopic cholecystectomy.

Conclusion
The preoperative scoring is statistically and clinically a good test for predicting the difficult level of laparascopic cholecystectomy (area under ROC = 0.948). The modified intraoperative measure of LC score is a statistically and clinically a good test for classifying the operative outcome of LC (area under ROC = 0.94).