bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Piezo channel is a mechano-sensitive complex component in the mammalian inner ear hair cell
Authors: Lee Jeong Han , Perez-Flores Maria C. , Park Seojin , Kim Hyo Jeong , Chen Yingying , Kang Mincheol , Kersigo Jennifer , Choi Jinsil , Thai Phung N. , Woltz Ryan L. , Perez-Flores Dolores Columba , Perkins Guy , Sihn Choong-Ryoul , Trinh Pauline , Zhang Xiao-Dong , Sirish Padmini , Dong Yao , Feng Wayne Wei , Pessah Isaac N. , Dixon Rose E. , Sokolowski Bernd , Fritzsch Bernd , Chiamvimonvat Nipavan , Yamoah Ebenezer N. 
Published: January 2024

AbstractThe inner ear is the hub where hair cells (HCs) transduce sound, gravity, and head acceleration stimuli to the brain. Hearing and balance rely on mechanosensation, the fastest sensory signals transmitted to the brain. The mechanoelectrical transducer (MET) channel is the entryway for the sound-balance-brain interface, but the channel-complex composition is not entirely known. Here, we report that the mouse utilizes Piezo1 (Pz1) and Piezo2 (Pz2) isoforms as MET-complex components. The Pz channels, expressed in HC stereocilia, and cell lines are co-localized and co-assembled with MET complex partners. Mice expressing non-functional Pz1 and Pz2 at the ROSA26 locus have impaired auditory and vestibular traits that can only be explained if the Pzs are integral to the MET complex. We suggest that Pz subunits constitute part of the MET complex and that interactions with other MET complex components yield functional MET units to generate HC MET currents.
Neuromorphic hardware for somatosensory neuroprostheses
Authors: Donati Elisa , Valle Giacomo 
Published: January 2024

AbstractIn individuals with sensory-motor impairments, missing limb functions can be restored using neuroprosthetic devices that directly interface with the nervous system. However, restoring the natural tactile experience through electrical neural stimulation requires complex encoding strategies. Indeed, they are presently limited in effectively conveying or restoring tactile sensations by bandwidth constraints. Neuromorphic technology, which mimics the natural behavior of neurons and synapses, holds promise for replicating the encoding of natural touch, potentially informing neurostimulation design. In this perspective, we propose that incorporating neuromorphic technologies into neuroprostheses could be an effective approach for developing more natural human-machine interfaces, potentially leading to advancements in device performance, acceptability, and embeddability. We also highlight ongoing challenges and the required actions to facilitate the future integration of these advanced technologies.
NAD+ dependent UPRmt activation underlies intestinal aging caused by mitochondrial DNA mutations
Authors: Yang Liang , Ruan Zifeng , Lin Xiaobing , Wang Hao , Xin Yanmin , Tang Haite , Hu Zhijuan , Zhou Yunhao , Wu Yi , Wang Junwei , Qin Dajiang , Lu Gang , Loomes Kerry M. , Chan Wai-Yee , Liu Xingguo 
Published: January 2024

AbstractAging in mammals is accompanied by an imbalance of intestinal homeostasis and accumulation of mitochondrial DNA (mtDNA) mutations. However, little is known about how accumulated mtDNA mutations modulate intestinal homeostasis. We observe the accumulation of mtDNA mutations in the small intestine of aged male mice, suggesting an association with physiological intestinal aging. Using polymerase gamma (POLG) mutator mice and wild-type mice, we generate male mice with progressive mtDNA mutation burdens. Investigation utilizing organoid technology and in vivo intestinal stem cell labeling reveals decreased colony formation efficiency of intestinal crypts and LGR5-expressing intestinal stem cells in response to a threshold mtDNA mutation burden. Mechanistically, increased mtDNA mutation burden exacerbates the aging phenotype of the small intestine through ATF5 dependent mitochondrial unfolded protein response (UPRmt) activation. This aging phenotype is reversed by supplementation with the NAD+ precursor, NMN. Thus, we uncover a NAD+ dependent UPRmt triggered by mtDNA mutations that regulates the intestinal aging.
Higher local Ebola incidence causes lower child vaccination rates
Authors: Das Upasak , Fielding David 
Published: January 2024

AbstractEbola is a highly infectious and often fatal zoonotic disease endemic to West and Central Africa. Local outbreaks of the disease are common, but the largest recorded Ebola epidemic originated in Guinea in December 2013, spreading to Liberia, and Sierra Leone in the following year and lasting until April 2016. The epidemic presented a serious challenge to local healthcare systems and foreign aid agencies: it degraded services, caused the loss of healthcare professionals, disrupted the economy, and reduced trust in modern healthcare. This study aims to estimate the extent to which variation in one long-term measure of the quality of local healthcare (the child vaccination rate) is a consequence of local variation in the intensity of the epidemic. Applying a “difference-in-differences” model to household survey data from before and after the epidemic, we show that in 2018–2019, overall rates of vaccination for BCG, DPT, measles, and polio are lower in Guinean and Sierra Leonean districts that had a relatively high incidence of Ebola; statistical analysis indicates that this is a causal effect. The effects of the epidemic on access to healthcare have been local effects, at least in part.
Decoration of Ag nanoparticles on CoMoO4 rods for efficient electrochemical reduction of CO2
Authors: Ray Schindra Kumar , Dahal Rabin , Ashie Moses D. , Bastakoti Bishnu Prasad 
Published: January 2024

AbstractHydrothermal and photoreduction/deposition methods were used to fabricate Ag nanoparticles (NPs) decorated CoMoO4 rods. Improvement of charge transfer and transportation of ions by making heterostructure was proved by cyclic voltammetry and electrochemical impedance spectroscopy measurements. Linear sweep voltammetry results revealed a fivefold enhancement of current density by fabricating heterostructure. The lowest Tafel slope (112 mV/dec) for heterostructure compared with CoMoO4 (273 mV/dec) suggested the improvement of electrocatalytic performance. The electrochemical CO2 reduction reaction was performed on an H-type cell. The CoMoO4 electrocatalyst possessed the Faraday efficiencies (FEs) of CO and CH4 up to 56.80% and 19.80%, respectively at  − 1.3 V versus RHE. In addition, Ag NPs decorated CoMoO4 electrocatalyst showed FEs for CO, CH4, and C2H6 were 35.30%, 11.40%, and 44.20%, respectively, at the same potential. It is found that CO2 reduction products shifted from CO/CH4 to C2H6 when the Ag NPs deposited on the CoMoO4 electrocatalyst. In addition, it demonstrated excellent electrocatalytic stability after a prolonged 25 h amperometric test at  − 1.3 V versus RHE. It can be attributed to a synergistic effect between the Ag NPs and CoMoO4 rods. This study highlights the cooperation between Ag NPs on CoMoO4 components and provides new insight into the design of heterostructure as an efficient, stable catalyst towards electrocatalytic reduction of CO2 to CO, CH4, and C2H6 products.
A systematic review and meta-analysis of the relationship between magnesium levels and malaria severity
Authors: Kotepui Kwuntida Uthaisar , Mahittikorn Aongart , Wilairatana Polrat , Masangkay Frederick Ramirez , Kotepui Manas 
Published: January 2024

AbstractMagnesium is associated with Plasmodium infections and malaria severity. This systematic review and meta-analysis was conducted to synthesize the link between Plasmodium infections and magnesium levels for improved clinical guidance and therapeutic interventions in malaria-affected regions. A systematic literature search was conducted across multiple databases, including ProQuest, Scopus, Embase, Ovid, MEDLINE, PubMed, and Google Scholar. The risk of bias in the selected studies was assessed using the Joanna Briggs Institute critical appraisal tools. A thematic synthesis was employed to demonstrate the magnesium levels across selected studies, for analyzing and grouping based on geographic regions, age demographics, and clinical manifestations of malaria. Meta-analyses determined differences in magnesium levels between individuals with malaria, uninfected controls, and patients with different clinical severities of malaria. The effect sizes from individual studies were pooled using the random-effects model. Out of 2533 records identified, 13 studies were included in the review. The thematic synthesis revealed complex and varied results, with studies showing different magnesium levels in malaria patients across different geographies, age groups, and clinical presentations. The meta-analysis indicated elevated magnesium levels in malaria patients compared with uninfected controls (P < 0.01, Hedges’ g: 1.94, 95% CI 0.86–3.03, I2: 98.38%, 9 studies). No statistically significant difference was observed in magnesium levels between patients with severe and nonsevere malaria (P: 0.34, Hedges’ g: 0.62, 95% CI − 0.64–1.88, I2: 91.46%, 2 studies). A significant increase in magnesium levels was seen in patients with malaria who died compared with those who survived (P < 0.01, Hedges’ g: 0.39, 95% CI 0.13–0.64, I2: 3.39%, 3 studies). This systematic review and meta-analysis presented relationship between magnesium levels and malaria. While the meta-analysis indicated a general trend of increased magnesium levels in patients with malaria, the substantial heterogeneity and instability of the results hint toward a rich yet uncharted territory requiring more research depth. The intricate interplay between magnesium levels and malaria beckons a multidimensional approach in future studies.
Transcriptional dysregulation of autophagy in the muscle of a mouse model of Duchenne muscular dystrophy
Authors: Nakashima Ryuta , Hosoda Ryusuke , Tatekoshi Yuki , Iwahara Naotoshi , Saga Yukika , Kuno Atsushi 
Published: January 2024

AbstractIt has been reported that autophagic activity is disturbed in the skeletal muscles of dystrophin-deficient mdx mice and patients with Duchenne muscular dystrophy (DMD). Transcriptional regulations of autophagy by FoxO transcription factors (FoxOs) and transcription factor EB (TFEB) play critical roles in adaptation to cellular stress conditions. Here, we investigated whether autophagic activity is dysregulated at the transcription level in dystrophin-deficient muscles. Expression levels of autophagy-related genes were globally decreased in tibialis anterior and soleus muscles of mdx mice compared with those of wild-type mice. DNA microarray data from the NCBI database also showed that genes related to autophagy were globally downregulated in muscles from patients with DMD. These downregulated genes are known as targets of FoxOs and TFEB. Immunostaining showed that nuclear localization of FoxO1 and FoxO3a was decreased in mdx mice. Western blot analyses demonstrated increases in phosphorylation levels of FoxO1 and FoxO3a in mdx mice. Nuclear localization of TFEB was also reduced in mdx mice, which was associated with elevated phosphorylation levels of TFEB. Collectively, the results suggest that autophagy is disturbed in dystrophin-deficient muscles via transcriptional downregulation due to phosphorylation-mediated suppression of FoxOs and TFEB.
Comprehensive genomic exploration of class III peroxidase genes in guava unravels physiology, evolution, and postharvest storage responses
Authors: Gull Shaista , Ali Muhammad Moaaz , Ejaz Shaghef , Ali Sajid , Rasheed Majeeda , Yousef Ahmed Fathy , Stępień Piotr , Chen Faxing 
Published: January 2024

AbstractPeroxidases (PRXs) play multifaceted roles in plant growth, development, and stress responses. Here, we present a comprehensive analysis of the PRX gene family in guava, a globally significant fruit. In the guava genome, we identified 37 PRX genes, a number lower than that of Arabidopsis, suggesting a distinctive gene family expansion pattern. Phylogenetic analysis unveiled close relationships with Arabidopsis PRXs, with 12 PgPRX genes forming ortholog pairs, indicating a specific expansion pattern. Predictions placed most PRX proteins in the chloroplast and extracellular regions. Structural analysis of PgPRX proteins revealed commonalities in domain structures and motif organization. Synteny analysis underscored the dynamic role of segmental duplication in the evolution of guava's PRX genes. We explored the dynamic expression of PgPRX genes across guava tissues, exposing functional diversity. Furthermore, we examined changes in peroxidase levels and gene expressions during postharvest fruit storage, providing insights for preserving fruit quality. This study offers an initial genome-wide identification and characterization of Class III peroxidases in guava, laying the foundation for future functional analyses.
Analysis of vitamin D receptor binding affinities of enzymatically synthesized triterpenes including ambrein and unnatural onoceroids
Authors: Ueda Daijiro , Matsuda Natsu , Takaba Yuka , Hirai Nami , Inoue Mao , Kameya Taichi , Abe Tohru , Tagaya Nao , Isogai Yasuhiro , Kakihara Yoshito , Bartels Florian , Christmann Mathias , Shinada Tetsuro , Yasuda Kaori , Sato Tsutomu 
Published: January 2024

AbstractOnoceroids are a rare family of triterpenes. One representative onoceroid is ambrein, which is the main component of ambergris used as a traditional medicine. We have previously identified the onoceroid synthase, BmeTC, in Bacillus megaterium and succeeded in creating ambrein synthase by introducing mutations into BmeTC. Owing to the structural similarity of ambrein to vitamin D, a molecule with diverse biological activities, we hypothesized that some of the activities of ambergris may be induced by the binding of ambrein to the vitamin D receptor (VDR). We demonstrated the VDR binding ability of ambrein. By comparing the structure–activity relationships of triterpenes with both the VDR affinity and osteoclastic differentiation-promoting activity, we observed that the activity of ambrein was not induced via the VDR. Therefore, some of the activities of ambergris, but not all, can be attributed to its VDR interaction. Additionally, six unnatural onoceroids were synthesized using the BmeTC reactions, and these compounds exhibited higher VDR affinity than that of ambrein. Enzymatic syntheses of onoceroid libraries will be valuable in creating a variety of bioactive compounds beyond ambergris.
Efficient synthesis of novel thiadiazolo[2,3-b]quinazolin-6-ones catalyzed by diphenhydramine hydrochloride-CoCl2⋅6H2O deep eutectic solvent
Authors: Korbekandi Mehri Moeini , Mohammadpoor-Baltork Iraj , Moghadam Majid , Tangestaninejad Shahram , Mirkhani Valiollah , Notash Behrouz 
Published: January 2024

AbstractIn this research, a new Lewis acid-based deep eutectic solvent (LA-DES) was synthesized using diphenhydramine hydrochloride and CoCl2·6H2O, (2[HDPH]:CoCl42−), and identified by FT-IR and 1HNMR techniques. The physicochemical properties of this LA-DES, such as thermal behavior, thermal stability, and solubility in common solvents were also investigated. The catalytic ability of 2[HDPH]:CoCl42− was ascertained in the efficient synthesis of a novel array of thiadiazolo[2,3-b]quinazolin-6-one scaffolds via a one-pot three-component reaction of dimedone/1,3-cyclohexanedione, aldehydes, and 5-aryl-1,3,4-thiadiazol-2-amines/3-(5-amino-1,3,4-thiadiazol-2-yl)-2H-chromen-2-one under solvent-free conditions. This catalyst was also successfully utilized for the synthesis of mono- and bis-thiadiazolo[2,3-b]quinazolin-6-ones from dialdehydes or bis-1,3,4-thiadiazol-2-amine. The simplicity of enforcement, short reaction time, avoidance of toxic organic solvents, scalability of the synthesis procedure, excellent atom economy, high reaction mass efficiency, and low E-factor are other outstanding advantages of this newly developed method. Furthermore, due to the convenient recovery and reuse of LA-DES, this protocol is economically justified and environmentally friendly.
Exploring the pathogenesis and key genes associated of acute myocardial infarction complicated with Alzheimer’s disease
Authors: Liu Chaosheng , Pan Fuzhi , Sun Zhiyu , Chen Ziyu , Wang Junjie 
Published: January 2024

AbstractDespite mounting evidence linking Acute Myocardial Infarction (AMI) to Alzheimer’s disease (AD), the shared mechanism of these two conditions’ occurrence remains unclear. This research aims to delve deeper into the molecular process of the occurrence of the two diseases. We retrieved the gene expression profiles of AD (GSE5281) and AMI (GSE66360) from the Gene Expression Omnibus database. Then, a total of 22 common differentially expressed genes (DEGs) including one downregulated gene and 21 upregulated genes were chosen for further analysis. Following the discovery of the common DEGs between AMI and AD, we performed protein–protein interaction analysis and hub gene identification analysis. Next, ten important hub genes were identified. Additionally, the key genes were identified by the least absolute shrinkage and selection operator and support vector machine‐recursive feature elimination and multivariable logistic regression analysis. The BCL6 was identified to be the most connected with AMI and AD. Finally, the BCL6 gene was validated in the GSE40680 (AMI) and GSE122063 (AD) datasets. Our research indicates that AMI and AD share a comparable pathophysiology. The Hub genes, especially BCL6, were essential in developing AMI and AD. In addition, these hub genes and shared pathways can offer fresh perspectives for additional mechanism investigation.
The Mediator kinase module enhances polymerase activity to regulate transcriptional memory after heat stress in Arabidopsis
Authors: Crawford Tim , Siebler Lara , Sulkowska Aleksandra , Nowack Bryan , Jiang Li , Pan Yufeng , Lämke Jörn , Kappel Christian , Bäurle Isabel 
Published: January 2024

AbstractPlants are often exposed to recurring adverse environmental conditions in the wild. Acclimation to high temperatures entails transcriptional responses, which prime plants to better withstand subsequent stress events. Heat stress (HS)-induced transcriptional memory results in more efficient re-induction of transcription upon recurrence of heat stress. Here, we identified CDK8 and MED12, two subunits of the kinase module of the transcription co-regulator complex, Mediator, as promoters of heat stress memory and associated histone modifications in Arabidopsis. CDK8 is recruited to heat-stress memory genes by HEAT SHOCK TRANSCRIPTION FACTOR A2 (HSFA2). Like HSFA2, CDK8 is largely dispensable for the initial gene induction upon HS, and its function in transcriptional memory is thus independent of primary gene activation. In addition to the promoter and transcriptional start region of target genes, CDK8 also binds their 3ʹ-region, where it may promote elongation, termination, or rapid re-initiation of RNA polymerase II (Pol II) complexes during transcriptional memory bursts. Our work presents a complex role for the Mediator kinase module during transcriptional memory in multicellular eukaryotes, through interactions with transcription factors, chromatin modifications, and promotion of Pol II efficiency.
Multifunctional molecularly imprinted nanozymes with improved enrichment and specificity for organic and inorganic trace compounds
Authors: Ge Zhanyi , Zhao Yilin , Li Jiayi , Si Zhaobo , Du Wenbo , Su Haijia 
Published: January 2024

The Fe3O4 molecularly imprinted peroxidase nanozymes were developed by modelling the substrate channels of natural enzymes with controlled selectivity.
Losses after Harvesting and Management
Authors: Mohamed Aezeden , Liet Zachariah , Goru Donovan , David Japhet 
Published: January 2024

It is widely recognized that postharvest losses in agriculture have a significant impact on the sustainability and security of the world’s food supply. This essay aims to investigate the reasons behind these losses, their effects, and possible solutions. Several factors, such as insufficient infrastructure, inadequate storage facilities, improper handling procedures, and limited market access, are analyzed as potential causes of postharvest losses. The discussion primarily focuses on the negative consequences of these losses on farmers’ livelihoods, food supply, and resource utilization, as well as the economic, environmental, and social implications.
The paper suggests several strategies to minimize postharvest losses, including strengthening value chains, promoting appropriate technology for processing and storage, enhancing farmer education and training, and upgrading transportation and storage infrastructure. Additionally, the significance of market processes and policy interventions in reducing losses is highlighted. By reducing postharvest agricultural losses, it is expected that food security, resource efficiency, and sustainable development will be improved.
Digenic Inheritance of PROC and SERPINC1 Mutations Contributes to Multiple Sites Venous Thrombosis
Authors: Li Xiangui , Zhu Jiabao , lv Fanzhen , Ma Wenqi , Zhou Weimin , Zhang Wenwen 
Published: January 2024

AbstractVenous thromboembolism (VTE) represents a worldwide health challenge, impacting millions of people each year. The genesis of venous thrombosis is influenced in part by genetic components. Hereditary thrombosis is described as a genetically determined susceptibility to VTE. In the present study, a male patient was referred to our department presenting with multiple venous thrombosis events in different locations. Given a lack of identifiable risk factors, we aimed to investigate the possible genetic factor underlying venous thrombosis. Whole-exome sequencing was employed to examine genes linked to inherited thrombophilia in the proband. Putative variants were subsequently confirmed through Sanger sequencing within the family. The proband was identified as carrying two genetic mutations. One is the novel c.400G > C (p.E134Q) mutation affecting the final nucleotide of exon 5 in the PROC gene, potentially impacting splicing. The other is a previously reported heterozygous nonsense variant c.1016G > A (p.W339X) in the SERPINC1 gene. The proband inherited the former from her mother and the latter from her father. The presence of digenic inheritance in the patient reflects the complex phenotype of venous thrombosis and demonstrates the significance of an unbiased approach to detect pathogenic variants, especially in patients with a high risk of hereditary thrombosis.
Häufigkeit von chronischem Schmerz im Alter,
Schmerzmechanismen und Schmerzchronifizierung, Schmerzursachen und
Neuroplastizität – Besonderheiten beim älteren
Menschen
Authors: Lenth Marcel , Schwarz Hermann 
Published: January 2024

ZusammenfassungDie Häufigkeit chronischer Schmerzen steigt im höheren
Lebensalter, aber die Diagnose wird oft durch verschiedene Faktoren erschwert,
z. B. durch andere Krankheiten oder eingeschränkte geistige
Fähigkeiten der Patienten. Die häufigsten Ursachen chronischer
Schmerzzustände im Alter sind degenerative Prozesse im Körper
und altersassoziierter Abbau. Die Schmerzbeurteilung und -behandlung bei
älteren Menschen erfordert angepasste Methoden, um eine Untererfassung
aufgrund möglicher geistiger Einschränkungen der Patienten zu
vermeiden. Es ist wichtig, mögliche kognitive, emotionale und
psychologische Beeinträchtigungen und Beschwerden zu
berücksichtigen, um eine detaillierte und genaue Therapie einzuleiten.
Eine genaue Kontrolle des Therapieerfolges ist unerlässlich. Im
Folgenden wird ein Überblick über die Epidemiologie, die Arten
und die Erfassung von Schmerzen bei älteren Patienten gegeben.
Darüber hinaus werden die Neuroplastizität im Zusammenhang mit
chronischen Schmerzen, schmerzverstärkende Faktoren und das
Gebrechlichkeitssyndrom betrachtet.