bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural Shifts of the Parvovirus B19 Capsid Receptor-binding Domain:
A Peptide Study
Authors: Khrustalev Vladislav Victorovich , Stojarov Aleksander Nicolaevich , Akunevich Anastasia Aleksandrovna , Baranov Oleg Evgenyevich , Popinako Anna Vladimirovna , Samoilovich Elena Olegovna , Yermalovich Marina Anatolyevna , Semeiko Galina Valeryevna , Sapon Egor Gennadyevich , Cheprasova Victoria Igorevna , Shalygo Nikolai Vladimirovich , Poboinev Victor Vitoldovich , Khrustaleva Tatyana Aleksandrovna , Khrustaleva Olga Victorovna 
Published: December 2023

Background::
Binding appropriate cellular receptors is a crucial step of a lifecycle for
any virus. Structure of receptor-binding domain for a viral surface protein has to be determined before
the start of future drug design projects.

Objective::
Investigation of pH-induced changes in the secondary structure for a capsid peptide
with loss of function mutation can shed some light on the mechanism of entrance.

Methods::
Spectroscopic methods were accompanied by electrophoresis, ultrafiltration, and computational
biochemistry.

Results::
In this study, we showed that a peptide from the receptor-binding domain of Parvovirus
B19 VP1 capsid (residues 13-31) is beta-structural at pH=7.4 in 0.01 M phosphate buffer, but alpha-
helical at pH=5.0, according to the circular dichroism (CD) spectroscopy results. Results of infra-
red (IR) spectroscopy showed that the same peptide exists in both alpha-helical and beta-structural
conformations in partial dehydration conditions both at pH=7.4 and pH=5.0. In contrast, the
peptide with Y20W mutation, which is known to block the internalization of the virus, forms mostly
alpha-helical conformation in partial dehydration conditions at pH=7.4. According to our hypothesis,
an intermolecular antiparallel beta structure formed by the wild-type peptide in its tetramers
at pH=7.4 is the prototype of the similar intermolecular antiparallel beta structure formed
by the corresponding part of Parvovirus B19 receptor-binding domain with its cellular receptor
(AXL).

Conclusion::
Loss of function Y20W substitution in VP1 capsid protein prevents the shift into the
beta-structural state by way of alpha helix stabilization and the decrease of its ability to turn into
the disordered state.
Dielectric Properties of Ni-Co Nanoferrites Synthesized using Green
Synthesis Process
Authors: Dhanda Neetu , Thakur Preeti , Thakur Atul 
Published: December 2023

Aim:
A series of nickel doped cobalt (NixCo1-xFe2O4, x=0.0 to 1.0) were successfully synthesized using green synthesized process.

Methods:
Tensile strain of all positive slope samples observed from the W-H (Williamson
Hall) plot. It was discovered that as Ni-substitution increased, the dielectric constant (є’) increased from 148.07 to 243.62. Conversely, when the amount of Ni-substitution increases, the
dielectric loss (tan δ from 0.23 to 0.05), imaginary part (є’’ from 98.81 to 17.87), and ac conductivity (σac from 1.62 to 0.15) all decreases at 1MHz frequency.

objective:
The purpose of current work is to investigate Williamson Hall effect and dielectric properties to find out the effects of nickel doping on the vibrational mode and crystal structure of cobalt ferrite.

Results:
This demonstrates that when Ni-substitution increases, energy losses at high frequencies decrease. Dielectric constant and ac conductivity, of all samples act in accordance with
Koop's theory, the Maxwell-Wagner polarization procedure, and electron hopping

Conclusion:
This makes them suitable materials for high-frequency applications
Molecular Characterization of Maturase K Gene and Protein Prediction of Vanda spathulata (L.) - An Ayurinformatics Approaches
Authors: Rani J Jeline , V Nandagopalan 
Published: December 2023

Vanda spathulata (L.) Spreng. is an important plant of the orchid family of botanical and horticultural importance in the Ayurvedic medicinal system. The Maturase K (matK) gene of plant chloroplast is a strong marker in studying plant molecular systematics, evolution, and genetic polymorphism and is considered as the standard DNA barcode for plants. The gene has an important role in the phylogenetic reconstruction of terrestrial plants. The present study investigated the phylogenetic characterization of matK gene obtained from the plant species Vanda spathulata (L.) through extraction, purification, PCR amplification, and gel elution. The Nucleotide BLAST search revealed similarity with 103 sequences in various closely and distantly related orchid taxa. Mega software and a real-time divergence tree discovered Apostasia odorata has the primitive species for all the sequences including the Vanda spathulata which diverged about 114 million years ago. Limited gene flow was observed among the Vanda and related taxa and the Tajima neutrality test was negative indicating the purifying selection in the matK gene sequence, that evolutionary selection had played a major role rather than the neutral evolution. The species and related other species exhibited higher polymorphism at the nucleotide level. Secondary structure was predicted for the single-stranded matK gene of V. spathulata and the ancestor plant species A. odorata using the Zucker MFold web server and translated through a comparison of ORF with NCBI and BLASTN. The protein was modeled in SWISS MODELLER and compared between the two species. The sequence had high similarity with its ancestor gene sequence and the protein modeled showed complex structure than the ancestor protein as a result of significant evolutionary changes.
РОЛЬ ДИСФУНКЦІЙНИХ КОГНІТИВНИХ СХЕМ У ПАТОГЕНЕЗІ КОМОРБІДНИХ АФЕКТИВНИХ ПОРУШЕНЬ В ОСІБ З СОЦІАЛЬНОЮ ТРИВОГОЮ ТА СОЦІАЛЬНОЮ ФОБІЄЮ
Authors: Аврамчук Олександр 
Published: December 2023

Мета: емпірично дослідити вплив когнітивних дисфункційних схем на вираженість коморбідної афективної патології, на прикладі симптомів депресивного стану в осіб з соціальною фобією.
Методи. Пошук попередніх досліджень здійснювався за допомогою сервісів PubMed, Web-of-Science та Research Gate з фокусом уваги на дослідження соціальної фобії та коморбідних до неї депресивних станів. Емпіричне дослідження охопило показники вибірок з клінічно значущими ознаками депресивної симптомів згідно критеріїв DSM-5 – основна група: 737 осіб з вираженою соціальною тривогою та 582 особи, контрольна група, без ознак соціальної фобії. Результати оцінювалися за методиками YSQ-S, SPIN, PHQ-9 та відповідними субшкалами методики SCL-90-R.
Результати. Продемонстровано, що рівень вираженості симптомів депресивних порушень й станів в осіб з соціальним тривожним розладом вищий за контрольну групу. Суттєву відмінність також продемонстрували показники загальної тяжкості симптомів та індекс симптомологічного дистресу. Аналіз актуальних ранніх дисфункційних схем продемонстрував суттєві відмінності (p<0,05) у порівнянні з контрольною групою для наступних категорій: соціальної ізоляції/відчуження, емоційної депривації, дефективності/сорому, емоційна інгібіція, самопожертви, надвисоких стандартів, очікування неминучих невдач/некомпетентності. Внесок дисперсії у показники соціальної тривоги та депресивної симптоматики можуть бути пояснені когнітивним, емоційним та поведінковим реагуванням відповідно до схем: соціальної ізоляції/відчуження, надвисоких стандартів, дефективності/сорому та неминучих невдач/некомпетентності. Типові переконання, які є характерними для соціальної фобії та пов'язані з очікуванням негативної оцінки від інших, підсилюються негативними думками, які є характерними для депресивного настрою. Уникаючи прямих соціальних контактів через страх покинення, людина обирає непродуктивні стратегії захисної поведінки, такі як емоційна депривація та соціальне відсторонення, а також раціоналізує порушене почуття соціальної приналежності.
Висновки. Аналіз результатів емпіричного дослідження підтверджує тісний зв'язок між дисфункційними переконаннями та вираженістю ключової депресивної психопатології в осіб з соціальним тривожним розладом. Подальші дослідження можуть бути скеровані на розширення ролі ранніх дисфункційних схем у патогенезі коморбідних станів завдяки медіаційному аналізу ролі механізмів уникнення когнітивного, емоційного (негативного) чи поведінкового у здобутті коригуючого досвіду як аспекту психологічної негнучкості та порушень адаптації.
PELATIHAN PEMBUATAN IKLAN PENYULUHAN BERBASIS APLIKASI CANVA DI KECAMATAN SAKO PALEMBANG
Authors: Aprilyanti Selvia , Faritzie Hariman Al , Azhari Azhari 
Published: December 2023

Sebuah media dalam penyuluhan iklan suatu kegiatan atau barang salah satunya berbasis aplikasi canva. Canva merupakan salah satu aplikasi dibidang desain grafis. Berdasarkan survey dilapangan staf dan masyarakat diKecamatan Sako Kota Palembang belum memahami tentang media promosi secara mandiri menggunakan digital advertising berbasis aplikasi canva untuk mendesain dan membuat media promosi atau penyuluhan kegiatan. Untuk itu pelaksanaan kegiatan pengabdian kepada masyarakat ini dilaksanakan melalui penyuluhan dan pelatihan penggunaan aplikasi Canva. Dengan tujuan memberikan edukasi kepada staf kantor dalam membuat media penyuluhan atau promosi menggunakan digital advertising. Kegiatan ini diharapkan dapat membantu masyarakat dalam menyebarkan atau promosi kegiatannya melalaui media digital. Kegiatan pengabdian ini dilaksanakan di Kantor Lurah Kelurahan Sialang Kecamatan Sako Kota Palembang kepada mitra yang berjumlah 30 orang warga. Metode pelaksanaan terdiri atas beberapa tahapan antara lain Koordinasi dengan mitra, Penyuluhan Materi dan Pelatihan. Adapun hasil pelatihan ini adanya tingkat pemahaman mitra yang mengikuti kegiatan. Para peserta mempraktikkan langsung sesuai arahan materi pelatihan. Evaluasi kegiatan yang dilakukan yaitu pembagian kuisioner dengan beberapa indikator yaitu kemudahan penggunaan, kejelasan materi yang disampaikan, dan manfaat yang dirasakan oleh peserta. Darii total 30 peserta yang terdiri atas 33% peserta telah memahami sedikit materi dari penyuluhan dan 67% peserta sudah sangat paham dengan materi penyuluhan tentang pemanfaatan dan penggunaan aplikasi Canva. Evaluasi kegiatan yang dilakukan yaitu pembagian kuisioner dengan beberapa indikator yaitu kemudahan penggunaan, kejelasan materi yang disampaikan, dan manfaat yang dirasakan oleh peserta. Peningkatan signifikan terjadi pada pengetahuan mitra. Aplikasi Canva akan membantu mitra dalam membuat media penyuluhan yang menarik dan persuasif.
Effects of Teaching Materials on Learning Salt Factory Topics Based on Ethnoscience to Improve Mastery of Students Concepts and Life Skills
Authors: Sarwi Sarwi , Fathonah Siti , Anis Meutia , Rozi Fahrur , Wardani Sri , Winarto Winarto 
Published: December 2023

Understanding concepts and life skills is important for students to develop. This study aims to analize effects of teaching materials on learning salt factory topics based on ethnoscience to improve mastery of concepts and life skills. This research is a quasi-experimental study using a non-equivalent design. Data collection techniques in this study used tests to measure concepts and questionnaires to measure life skills. The research instruments were multiple choice tests and a Likert scale questionnaire. The instrument meets the validity and reliability. The sample in this study was 77 elementary school students. Data analysis technique used paired t-tests, and one ways ANOVA. The application of ethnoscience-based thematic learning tools in experimental 1 and experiment 2 showed higher concept understanding posttest results compared to control groups with conventional learning. This was proved by the sig value. < 0.05 and the average posttest score in experiment class 1 was 80.32 and experiment 2 was 83.37 higher than the control class of 75.25. learning physics salt factory materials sourced from local wisdom can increase mastery of concepts and increase understanding of life skills. This teaching effective enough in improving the mastery of concepts and developing students' life skills.
TANGGUNGJAWAB PENGUSAHA TAMBANG TERHADAP KESEHATAN MASYARAKAT AKIBAT DARI POLUSI YANG DITIMBULKAN
Authors: YANI AHMAD , Suryani Hamzah Any 
Published: December 2023

Penelitian ini bertujuan untuk mengetahui pengaturan tanggungjawab sosial perusahaan tambang menurut undang-undang, faktor-faktor penyebab dari dampak yang ditimbulkan serta tanggungjawab perusahaan atas dampak polusi yang ditimbulkan. Penelitian ini menggunakan jenis penelitian hukum empiris dengan metode analisis kualitatif melalui pendekatan perundang-undangan, konseptual dan sosiologis. Hasil penelitian menunjukkan bahwa undang-undang yang mengatur tanggungjawab sosial perusahaan tambang yaitu UU No 20/2020 Tentang Minerba, UU No 32/2009 Tentang lingkungan hidup, UU No 36/2009 Tentang kesehatan, UU No 17/2019 Tentang sumber daya air, PMK No 61/2013 Tentang Kesehatan Matra dan PP No 22/2021 Tentang lingkungan hidup. Adapun faktor penyebab dari dampak yang ditimbulkan yaitu berasal dari kegiatan pertambangan yang terbuka, minimnyanya drainase, lalu lintas kendaraan pengangkut hasil pertambangan serta pengolahaan hasil tambang yang terbuka tanpa penyiraman. Dampak yang ditimbulkan antara lain pencemaran udara, rusaknya jalan, berpotensi terjadinya erosi tanah dan penurunan kualitas air. Pertanggungjawaban perusahaan tambang atas dampak yang ditimbulkan yaitu dengan melakukan penyiraman dan penutupan bak kendaraan tambang, memberikan CSR, ganti rugi terhadap masyarakat yang lahan pertaniannya terkena dampak, melakukan reklamsi lahan bekas tambang serta pembuatan drainase.
Understanding the Warburg Effect Yields New Insights into the Metabolic Control of Cancer
Authors: Bahrami Hassan , Tafrihi Majid 
Published: December 2023

Human cells may use either aerobic or anaerobic cellular respiration processes to produce energy, depending on cellular conditions. When there is enough oxygen, cells respire aerobically, but in case of oxygen deficiency, anaerobic cellular respiration is used, which leads to lactic acidosis and an increased risk of cancer according to Warburg's hypothesis.
This paper reviews key aspects related to the historical evolutionary origins of metabolic pathways in cancer cells and compares similarities between cancer cells and ancient unicellular organisms to address the origins of metabolic change in cancer cells and provide new insights into the metabolic control of cancer.
Understanding the main causes of cancer and the biological origin of their behavioral abnormalities is essential for the metabolic control of cancer. Environmental stressors to cells may include lack of essential nutrients, poor oxygenation, excess acids, viruses, infections, and exposure to chemicals, toxins, and radiation. These cellular stressors can cause normal cells to mutate and become cancerous in an attempt to survive in the harsh conditions.
According to the research findings, creating appropriate conditions at the cellular level in terms of pH, sufficient oxygenation and the availability of good sugars, essential vitamins, minerals, enzymes and coenzymes through a healthy diet can lead to a metabolic switch in cancer cells that controls mutations, which can help prevent and control cancer.
Sanando a nuestros médicos: una reflexión sobre nuestra salud
Authors: Robinson Davis Eugenia C. 
Published: December 2023

Todos los médicos buscamos siempre apoyarnos en la evidencia científica y ampliar nuestros conocimientos para ponerlos al servicio de nuestros pacientes. Nuestros pacientes son nuestra razón de ser. Sin embargo es importante reflexionar sobre la vulnerabilidad de nuestra población médica con trastornos en la salud física y mental que van desde depresión, ansiedad, “burnout”, eventos coronarios, cerebrovasculares, neoplasias, demencia, suicidios y farmacodependencia entre otros. Es una morbilidad sentida a la cual no podemos seguir indiferentes.
Estas situaciones no solo dependen de nuestra información genética, sino que también son desencadenados en gran medida por el estrés generado por las cargas depositadas en nosotros, las angustias y dolencias de nuestros pacientes, las presiones de las instituciones y del sistema de salud sumado a nuestros propios avatares internos. Como consecuencia se va deteriorando no solo nuestra salud sino también la empatía con el paciente la cual más allá del conocimiento científico es esencial para su recuperación. Esta situación no solo afecta a los médicos en ejercicio sino también a los médicos en formación.
Este artículo les ofrece a mis queridos colegas una reflexión sobre algunas herramientas que están a nuestro alcance para reducir dicha vulnerabilidad y continuar ejerciendo esta maravillosa profesión.
Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues.
Authors: Lelo Abdelameer K. , Abdulmohaimen Nidhal , Aftan Faeza 
Published: December 2023

Background: Uncontrolled tumor cell proliferation is a reality in tumor cells, and the progression from a normal cell into a transformed cell probably includes genetic events affecting checkpoints in the cell cycle machinery.Materials and methods: This study investigated the immunoexpression of p16INK4a in the paraffin sections from 43-cancers of colorectal tissue (CRC),26-hyperplastic polyps and adenomas(CRHPA) and 35-normal tissues (CRN),using immunohistochemical assay. We correlated the expression patterns with tumor histopathological type, site of the tumor, distance metastasis according to the TNM system.Results: In colorectal cancer (CRC) patients’ p16INK4a,immunoexpression was detected in 32/43, there was significant association for p16INK4a, which showed high reaction with monoclonal antibodies in the distal colon and rectal area with P value < 0.001. There was no significant correlation between tissue invasion stage and p16INK4a. There was no significant difference in the immunoexpression of p16INK4a in relation to the two groups of patients with the stage of the disease.Conclusion: p16INK4a, expression have high level in CRHPA than in CRN tissue. Also, their expression in CRC is more than in CRN tissue. In addition, p16INK4a immunoexpression in CRC was significantly more than in CRHPA.
Ocular pulse amplitude (OPA) in canine ADAMTS10-open-angle glaucoma (ADAMTS10-OAG)
Authors: Raphtis Vanessa A. , Sharma Dhruv , Wang Sichao , Kim Jae Y. , Jacobson Amanda L. , Harman Christine D. , Komáromy András M. 
Published: December 2023

Introduction: The role of ocular rigidity and biomechanics remains incompletely understood in glaucoma, including assessing an individual’s sensitivity to intraocular pressure (IOP). In this regard, the clinical assessment of ocular biomechanics represents an important need. The purpose of this study was to determine a possible relationship between the G661R missense mutation in the ADAMTS10 gene and the ocular pulse amplitude (OPA), the difference between diastolic and systolic intraocular pressure (IOP), in a well-established canine model of open-angle glaucoma (OAG).Methods: Animals studied included 39 ADAMTS10-mutant dogs with different stages of OAG and 14 unaffected control male and female dogs between 6 months and 12 years (median: 3.2 years). Dogs were sedated intravenously with butorphanol tartrate and midazolam HCl, and their IOPs were measured with the Icare® Tonovet rebound tonometer. The Reichert Model 30™ Pneumotonometer was used to measure OPA. Central corneal thickness (CCT) was measured via Accutome® PachPen, and A-scan biometry was assessed with DGH Technology Scanmate. All outcome measures of left and right eyes were averaged for each dog. Data analysis was conducted with ANOVA, ANCOVA, and regression models.Results:ADAMTS10-OAG-affected dogs displayed a greater IOP of 23.0 ± 7.0 mmHg (mean ± SD) compared to 15.3 ± 3.6 mmHg in normal dogs (p &lt; 0.0001). Mutant dogs had a significantly lower OPA of 4.1 ± 2.0 mmHg compared to 6.5 ± 2.8 mmHg of normal dogs (p &lt; 0.01). There was no significant age effect, but OPA was correlated with IOP in ADAMTS10-mutant dogs.Conclusion: The lower OPA in ADAMTS10-mutant dogs corresponds to the previously documented weaker and biochemically distinct posterior sclera, but a direct relationship remains to be confirmed. The OPA may be a valuable clinical tool to assess ocular stiffness and an individual’s susceptibility to IOP elevation.
Impact of Baizhu, Daqingye, and Hehuanhua extracts on the human gut microbiome
Authors: Lemons Johanna M. S. , Narrowe Adrienne B. , Liu LinShu , Firrman Jenni , Mahalak Karley K. , Van den Abbeele Pieter , Baudot Aurélien , Deyaert Stef , Li Yanfang , Yu Liangli (Lucy) 
Published: December 2023

IntroductionIn traditional Chinese medicine, the rhizome of Atractylodes macrocephala (Baizhu), the leaves of Isatis indigotica (Daqingye), and the flowers of Albizia julibrissin (Hehuanhua) have been used to treat gastrointestinal illnesses, epidemics, and mental health issues. Modern researchers are now exploring the underlying mechanisms responsible for their efficacy. Previous studies often focused on the impact of purified chemicals or mixed extracts from these plants on cells in tissue culture or in rodent models.MethodsAs modulation of the human gut microbiome has been linked to host health status both within the gastrointestinal tract and in distant tissues, the effects of lipid-free ethanol extracts of Baizhu, Daqingye, and Hehuanhua on the human adult gut microbiome were assessed using Systemic Intestinal Fermentation Research (SIFR®) technology (n=6).Results and discussionBaizhu and Daqingye extracts similarly impacted microbial community structure and function, with the extent of effects being more pronounced for Baizhu. These effects included decreases in the Bacteroidetes phylum and increases in health-related Bifidobacterium spp. and short chain fatty acids which may contribute to Baizhu’s efficacy against gastrointestinal ailments. The changes upon Hehuanhua treatment were larger and included increases in multiple bacterial species, including Agathobaculum butyriciproducens, Adlercreutzia equolifaciens, and Gordonibacter pamelaeae, known to produce secondary metabolites beneficial to mental health. In addition, many of the changes induced by Hehuanhua correlated with a rise in Enterobacteriaceae spp., which may make the tested dose of this herb contraindicated for some individuals. Overall, there is some evidence to suggest that the palliative effect of these herbs may be mediated, in part, by their impact on the gut microbiome, but more research is needed to elucidate the exact mechanisms.