bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insights into Yeast Response to Chemotherapeutic Agent through Time Series Genome-Scale Metabolic Models
Authors: Karabekmez Muhammed Erkan 
Published: January 2024

AbstractBackgroundOrganism-specific genome-scale metabolic models (GSMMs) can unveil molecular mechanisms within cells and are commonly used in diverse applications, from synthetic biology, biotechnology, and systems biology to metabolic engineering. There are limited studies incorporating time-series transcriptomics in GSMM simulations. Yeast is an easy-to-manipulate model organism for tumor research;MethodsHere, a novel approach (TS-GSMM) was proposed to integrate time-series transcriptomics with GSMMs to narrow down the feasible solution space of all possible flux distributions and attain time-series flux samples. The flux samples were clustered using machine learning techniques, and the clusters’ functional analysis was performed using reaction set enrichment analysis;ResultsA time series transcriptomics response of Yeast cells to a chemotherapeutic reagent – doxorubicin - was mapped onto a Yeast GSMM. Eleven flux clusters were obtained with our approach, and pathway dynamics were displayed. Induction of fluxes related to bicarbonate formation and transport, ergosterol and spermidine transport, and ATP production were captured;ConclusionsIntegrating time-series transcriptomics data with GSMMs is a promising approach to reveal pathway dynamics without any kinetic modeling and detects pathways that cannot be identified through transcriptomics-only analysis. The codes are available athttps://github.com/karabekmez/TS-GSMM.
Antigen density and applied force control enrichment of nanobody-expressing yeast cells in microfluidics
Authors: Sanicas Merlin , Torro Rémy , Limozin Laurent , Chames Patrick 
Published: January 2024

AbstractIn vitro display technologies such as yeast display have been instrumental in developing the selection of new antibodies, antibody fragments or nanobodies that bind to a specific target, with affinity towards the target being the main factor that influences selection outcome. However, the roles of mechanical forces are being increasingly recognized as a crucial factor in the regulation and activation of effector cell function. It would thus be of interest to isolate binders behaving optimally under the influence of mechanical forces. We developed a microfluidic assay allowing the selection of yeast displaying nanobodies through antigen-specific immobilization on a surface under controlled hydrodynamic flow. This approach enabled enrichment of model yeast mixtures using tunable antigen density and applied force. This new force-based selection method opens the possibility of selecting binders by relying on both their affinity and force resistance, with implications for the design of more efficient immunotherapeutics.
Contrast-enhanced photon-counting micro-CT of tumor xenograft models
Authors: Li Mengzhou , Guo Xiaodong , Verma Amit , Rudkouskaya Alena , McKenna Antigone M. , Intes Xavier , Wang Ge , Barroso Margarida 
Published: January 2024

AbstractPhoton-counting micro computed tomography (micro-CT) offers new potential in preclinical imaging, particularly in distinguishing materials. It becomes especially helpful when combined with contrast agents, enabling the differentiation of tumors from surrounding tissues. There are mainly two types of contrast agents in the market for micro-CT: small molecule-based and nanoparticle-based. However, despite their widespread use in liver tumor studies, there is a notable gap in research on the application of these commercially available agents for photon-counting micro-CT in breast and ovarian tumors. Herein, we explored the effectiveness of these agents in differentiating tumor xenografts from various origins (AU565, MDA-MB-231, and SKOV-3) in nude mice, using photon-counting micro-CT. Specifically, ISOVUE-370 (a small molecule-based agent) and Exitrone Nano 12000 (a nanoparticle-based agent) were investigated in this context. To improve tumor visualization, we proposed a novel color visualization method for photon-counting micro-CT, which changes color tones to highlight contrast media distribution, offering a robust alternative to traditional material decomposition methods with less computational demand. Ourin vivoexperiments confirm its effectiveness, showing distinct enhancement characteristics for each contrast agent. Qualitative and quantitative analyses suggested that Exitrone Nano 12000 provides superior vasculature enhancement and better quantitative consistency across scans, while ISOVUE-370 gives more comprehensive tumor enhancement but with a significant variance between scans due to its short blood half-time. This variability leads to high sensitivity to timing and individual differences among mice. Further, a paired t-test on mean and standard deviation values within tumor volumes showed significant differences between the AU565 and SKOV-3 tumor models with the nanoparticle-based (p-values < 0.02), attributable to their distinct vascularity, as confirmed by immunohistochemistry. These findings underscore the utility of photon-counting micro-CT in non-invasively assessing the morphology and anatomy of different tumor xenografts, which is crucial for tumor characterization and longitudinal monitoring of tumor development and response to treatments.
Dietary methionine/choline deficiency affects behavioural and molecular circadian rhythms
Authors: Saer Benjamin , Taylor George , Hayes Andrew , Ananthasubramaniam Bharath , Fustin Jean-Michel 
Published: January 2024

AbstractThe feeding/fasting cycles controlled by our circadian clock impose great daily metabolic and physiological changes, and yet investigations into the consequences of metabolic deficiencies, either dietary or genetic, have often ignored the time of day or the circadian time of the animals or subjects. In addition, these deficiencies may themselves disrupt our circadian clock, causing secondary metabolic, physiological and behavioural disorders.Dietary methionine/choline deficiency in rodents is a common model for human non-alcoholic steatohepatitis, but methionine and choline are nutrients essential for many other processes beyond fatty acid synthesis in the liver, including biological methylations and 1-carbon metabolism, regulation of translation notably via the mTOR pathway, phospholipid synthesis, polyamine pathway and glutathione synthesis.We have previously shown that circadian rhythms in many organisms are highly sensitive to deficiency or excesses of 1-carbon metabolites. Using a methionine/choline deficient diet in mice, we illustrate the nutrigenomic crosstalk between circadian rhythms and 1-carbon metabolism. We show not only that circadian locomotor activity behaviour is profoundly, rapidly and reversibly affected by methionine/choline deficiency, but also that the effects of methionine/choline deficiency on gene expression and 1-carbon metabolites are dependent on circadian time, illustrating the importance of considering circadian rhythms in metabolic studies. This study also highlights the impact of what we eat, or don’t, on our behaviour and biological rhythms.
Selfish behavior requires top-down control of prosocial motivation
Authors: Numano Shotaro , Frith Chris , Haruno Masahiko 
Published: January 2024

AbstractIndividuals must regularly choose between prosocial and proself behaviors. While past neuroscience research has revealed the neural foundations for prosocial behaviors, many studies have oversimplified proself behaviors, viewing them merely as a reward-maximization process. However, recent behavioral evidence suggests that response times for proself behaviors are often slower than those for prosocial behaviors, suggesting a more complex interdependence between prosocial and proself neural computations. To address this issue, we conducted an fMRI experiment with the ultimatum game, where participants were requested to accept (money distributed as proposed) or reject (both sides receive none) offers of money distribution. In the decisions, the participants could maximize self-interest by accepting the offer (i.e., proself), while by rejecting it, they could punish unfair proposers and promote the “equity” social norm (i.e., prosocial). We constructed a drift diffusion model (DDM) that considers both behavioral choices and response times and used the DDM parameters in our fMRI analysis. We observed that participants who suppressed inequity-driven rejection behaviors displayed heightened dACC activity in response to disadvantageous inequity. Importantly, our functional connectivity analysis demonstrated that the dACC exhibited negative functional connectivity with the amygdala when unfair offers were presented. Furthermore, the PPI connectivity encoded the average reaction time for accepting unfair offers (i.e., proself behaviors). Considering that the amygdala also responded to disadvantageous inequity in these experiments and previous studies, these results show that the top-down control of prosocial motives (i.e., aversion to disadvantageous inequity) plays a key role in implementing proself behaviors.
Linking brain-heart interactions to emotional arousal in immersive virtual reality
Authors: Fourcade A. , Klotzsche F. , Hofmann S. M. , Mariola A. , Nikulin V. V. , Villringer A. , Gaebler M. 
Published: January 2024

AbstractThe subjective experience of emotions is linked to the contextualized perception and appraisal of changes in bodily (e.g., heart) activity. Increased emotional arousal has been related to attenuated high-frequency heart rate variability (HF-HRV), lower EEG parieto-occipital alpha power, and higher heartbeat-evoked potential (HEP) amplitudes. We studied emotional arousal-related brain- heart interactions using immersive virtual reality (VR) for naturalistic yet controlled emotion induction. 29 healthy adults (13 women, age: 26±3) completed a VR experience that included rollercoasters while EEG and ECG were recorded. Continuous emotional arousal ratings were collected during a video replay immediately after. We analyzed emotional arousal-related changes in HF-HRV as well as in BHIs using HEPs. Additionally, we used the oscillatory information in the ECG and the EEG to model the directional information flows between the brain and heart activity.We found that higher emotional arousal was associated with lower HEP amplitudes in a left fronto- central electrode cluster. While parasympathetic modulation of the heart (HF-HRV) and parieto- occipital EEG alpha power were reduced during higher emotional arousal, there was no evidence for the hypothesized emotional arousal-related changes in bidirectional information flow between them. Whole-brain exploratory analyses in additional EEG (delta, theta, alpha, beta and gamma) and HRV (low-frequency, LF, and HF) frequency bands revealed a temporo-occipital cluster, in which higher emotional arousal was linked to decreased brain-to-heart (i.e., gamma→HF-HRV) and increased heart-to-brain (i.e., LF-HRV→gamma) information flow. Our results confirm previous findings from less naturalistic experiments and suggest a link between emotional arousal and brain-heart interactions in temporo-occipital gamma power.
Developmental Reprogramming of Hypothalamic-Pituitary Axis in Mice by Common Environmental Pollutants
Authors: Mogus Joshua P. , Marin Marjorie , Arowolo Olatunbosun , Salemme Victoria , Suvorov Alexander 
Published: January 2024

AbstractHumans are exposed to a large number of endocrine disrupting chemicals (EDCs). Many studies demonstrated that exposures to EDCs during critical windows of development can permanently affect endocrine health outcomes. Most of experimental studies address changes in secretion of hormones produced by gonads, thyroid gland and adrenals, and little is known about the ability of EDCs to produce long-term changes in the hypothalamic-pituitary (HP) control axes. Here, we examined the long-term effects of three common EDCs on male mouse HP gene expression, following developmental exposures. Pregnant mice were exposed to 0.2 mg/ml solutions of bisphenol S (BPS), 2,2’,4,4’-tetrabromodiphenyl ether (BDE-47), or 3,3’,5,5’-tetrabromobisphenol A (TBBPA) from pregnancy day 8 through lactation day 21 (weaning day). Male offspring were left untreated until postnatal day 140, where pituitaries and hypothalami were collected. Pituitaries were assed for gene expression via RNA sequencing, while specific genes were assessed for expression in hypothalami via RT-qPCR. Differential expression, as well as gene enrichment and pathway analysis, indicated that all three chemicals induced long-term changes, (mostly suppression) in pituitary genes involved in its endocrine function. BPS and BDE-47 produced effects overlapping significantly at the level of effected genes and pathways. All three chemicals altered genes and pathways of gonad and liver HP axes, while BPS altered HP-adrenal and BDE-47 altered HP-thyroid pathways specifically. All three chemicals also reduced expression of immune genes in the pituitaries. Targeted gene expression in the hypothalamus indicates a down regulation of hypothalamic endocrine control genes by BPS and BDE-47 groups, concordant with changes in the pituitary and suggests that these chemicals suppress the overall HP endocrine function. Interestingly, all three chemicals altered pituitary genes of GPCR-mediated intracellular signaling molecules, many of which are key signalers common to many pituitary responses to hormones. The results of this study show that developmental exposures to common and ubiquitous EDCs have long-term impacts on hormonal feedback control at the hypothalamic-pituitary level.
Femtosecond-laser direct-write photoconductive patterns on tellurite glass
Authors: Torun Gözden , Romashkina Anastasia , Kishi Tetsuo , Bellouard Yves 
Published: January 2024

We report the formation of arbitrary photoconductive patterns made of tellurium (Te) nanocrystals by exposing a tellurite (TeO2-based) glass to femtosecond laser pulses. During this process, Te/TeO2-glass nanocomposite interfaces with photoconductive properties form on the tellurite glass substrate. We show that these laser-written patterns exhibit a photoresponse, from the near ultraviolet (263 nm) to the visible spectrum, stable over a few months. Specifically, high responsivity (16.55 A/W) and detectivity (5.25 × 1011 Jones) of a single laser-written line pattern are measured for an illumination dose of 0.07 mW/cm2 at 400 nm. This work illustrates a pathway for locally turning a tellurite glass into a functional photoconductor of arbitrary shape, without adding materials and using a single laser process step.

Published by the American Physical Society
2024
The IFRS 16 implementation in Asia-Pacific countries: enhancing asset pronouncements or opaque information’s conveyance
Authors: Utami Evy Rahman , Sumiyana Sumiyana , Mustakini Jogiyanto Hartono , Barokah Zuni 
Published: January 2024

Purpose
The purpose of this study is to investigate the implementation of International Financial Reporting Standard (IFRS) 16 in developing countries to enhance asset pronouncements or the quality of opaque accounting information for listed firms’ leasing transactions.

Design/methodology/approach
This study designed ordinary least square (OLS) regression models to examine the hypotheses in two ordered tests. The first-order test ascertained the association between fundamental accounting information and earnings or stock prices. Then, the second-order test was nested to add the instrument variable to the first-order one. In addition, the researchers selected 17 Asia-Pacific countries.

Findings
First, this study contributes to the fair value of firms’ asset measurements, and the accounting discipline requires adaptive scalability to produce future potential cash flows. Second, it reduces literature gaps between the pros and cons of the opaqueness of assets. In addition, these research arguments would be the referee for reducing information’s opacity. Finally, this study demonstrates the impact of IFRS 16’s implementation on firms’ conservatism levels and entropy’s information quality, requiring the regulators to accommodate these issues.

Originality/value
Due to the implementation of IFRS 16, the authors are neutral about the impacted financial statements and political consequences for these Asia-Pacific listed firms and countries. First, we propose the uniqueness of problematic elaboration since implementing IFRS 16 results in a more pronounced or opaque information quality due to vulnerable complexities in the financial statements. Second, this implementation is associated with hierarchical information and conservatism, producing accounting information entropy or negentropy. However, the hierarchy theory suggests various levels of conservatism that could increase or decrease the information’s quality.
Statistical analysis of radiative solar trough collectors for MHD Jeffrey hybrid nanofluid flow with gyrotactic microorganism: entropy generation optimization
Authors: Kumar Anup , Sharma Bhupendra Kumar , Bin-Mohsen Bandar , Fernandez-Gamiz Unai 
Published: January 2024

Purpose
A parabolic trough solar collector is an advanced concentrated solar power technology that significantly captures radiant energy. Solar power will help different sectors reach their energy needs in areas where traditional fuels are in use. This study aims to examine the sensitivity analysis for optimizing the heat transfer and entropy generation in the Jeffrey magnetohydrodynamic hybrid nanofluid flow under the influence of motile gyrotactic microorganisms with solar radiation in the parabolic trough solar collectors. The influences of viscous dissipation and Ohmic heating are also considered in this investigation.

Design/methodology/approach
Governing partial differential equations are derived via boundary layer assumptions and nondimensionalized with the help of suitable similarity transformations. The resulting higher-order coupled ordinary differential equations are numerically investigated using the Runga-Kutta fourth-order numerical approach with the shooting technique in the computational MATLAB tool.

Findings
The numerical outcomes of influential parameters are presented graphically for velocity, temperature, entropy generation, Bejan number, drag coefficient and Nusselt number. It is observed that escalating the values of melting heat parameter and the Prandl number enhances the Nusselt number, while reverse effect is observed with an enhancement in the magnetic field parameter and bioconvection Lewis number. Increasing the magnetic field and bioconvection diffusion parameter improves the entropy and Bejan number.

Originality/value
Nanotechnology has captured the interest of researchers due to its engrossing performance and wide range of applications in heat transfer and solar energy storage. There are numerous advantages of hybrid nanofluids over traditional heat transfer fluids. In addition, the upswing suspension of the motile gyrotactic microorganisms improves the hybrid nanofluid stability, enhancing the performance of the solar collector. The use of solar energy reduces the industry’s dependency on fossil fuels.
Unraveling the link between innovative work behavior and despotic leadership: the roles of supervisor conflict and dispositional resistance to change trait
Authors: Dar Nasib , Ahmad Saima , Badar Kamal , Kundi Yasir Mansoor 
Published: January 2024

Purpose
This paper aims to probe the prevailing belief that engaging in innovative work behavior (IWB) will invariably lead to favorable outcomes. To do so, the paper integrates followership theory and cognitive dissonance theory to investigate the connection between employees’ IWB and despotic leadership, and the mediating role of interpersonal conflict with the supervisor in this connection. Moreover, the moderating impact of the supervisor’s dispositional resistance to change trait on the direct and indirect relationship between IWB and despotic leadership is explored.

Design/methodology/approach
The data were collected through a multi-wave survey of 350 employees and 81 supervisors working in 81 public schools.

Findings
The findings show that IWB has a positive and significant relationship with despotic leadership, and this relationship is mediated by interpersonal conflict with the supervisor.

Originality/value
To the best of the authors’ knowledge, this is the first study that examines the link between IWB and despotic leadership via interpersonal/dyadic conflict and explores the moderating effect of leadership dispositional resistance to change trait in this indirect relationship.
Scholarship of teaching and learning at AACSB accredited business school: who’s doing it, and how’s it captured?
Authors: Siddiqui Sanobar , Lento Camillo 
Published: January 2024

PurposeThis paper explores who among the AACSB categorization of academics conducts the scholarship of teaching and learning (SoTL) research within business schools and how AACSB-accredited business schools capture SoTL research as part of their portfolio of intellectual contributions.Design/methodology/approachThis study adopts a qualitative-method research design by collecting primary data through surveys, semi-structured interviews and secondary data in policy documents focused on AACSB-accredited business schools in Canada and the United States.FindingsThe findings establish that scholarly and practice academics who possess rigorously acquired research skills due to their terminal degrees are most likely to conduct SoTL research. The results also reveal an even split among respondents regarding whether their AACSB-accredited business school captures SoTL with their journal ranking frameworks.Practical implicationsBased on the findings, two recommendations are offered to foster more SoTL research at AACSB-accredited schools. First, higher education leaders (e.g. business school deans) can further inculcate a culture of SoTL research at the department and institutional levels by creating communities of practice (CoPs). Second, AACSB-accredited business schools could adopt more inclusive journal ranking frameworks to capture better and incentivize SoTL research.Originality/valueThis is the first known study to explore how AACSB Standards 3 and 8 are implemented and operationalized regarding SoTL research. Understanding how these standards are adopted and implemented could help institutional leaders, standard setters and administrators better facilitate SoTL research.