bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological and therapeutic implications of RKIP in Gastrointestinal Stromal Tumor (GIST): an integrated transcriptomic and proteomic analysis
Authors: Campanella Nathália Cristina , Gomes Izabela Natalia Faria , Alves Ana Laura Vieira , Leal Leticia Ferro , Evangelista Adriane Feijó , Rosa Marcela Nunes , Melendez Matias Eliseo , Silva Viviane Aline Oliveira , Dias Richard Lucas Konichi , Abrahão-Machado Lucas Faria , Santana Iara , Martinho Olga , Guimarães Denise Peixoto , Faça Vitor Marcel , Reis Rui Manuel 
Published: October 2023

AbstractBackgroundGastrointestinal stromal tumors (GIST) represent a significant clinical challenge due to their metastatic potential and limited treatment options. Raf kinase inhibitor protein (RKIP), a suppressor of the MAPK signaling pathway, is downregulated in various cancers and acts as a metastasis suppressor. Our previous studies demonstrated low RKIP expression in GIST and its association with poor outcomes. This study aimed to expand on the previous findings and investigate the biological and therapeutic implications of RKIP loss on GIST.MethodsTo validate the RKIP prognostic significance, its expression was evaluated by immunohistochemistry in 142 bona fide GIST cases. The functional role of RKIP was evaluated in vitro, using the GIST-T1 cell line, which was knocked out for RKIP. The biological and therapeutic implications of RKIP were evaluated by invasion, migration, apoptosis, and 2D / 3D viability assays. Additionally, the transcriptome and proteome of RKIP knockout cells were determined by NanoString and mass spectrometry, respectively.ResultsImmunohistochemical analysis revealed the absence of RKIP in 25.3% of GIST cases, correlating with a tendency toward poor prognosis. Functional assays demonstrated that RKIP knockout increased GIST cells’ invasion and migration potential by nearly 60%. Moreover, we found that RKIP knockout cells exhibited reduced responsiveness to Imatinib treatment and higher cellular viability in 2D and 3D in vitro models, as assessed by apoptosis-related protein expression. Through comprehensive genetic and proteomic profiling of RKIP knockout cells, we identified several putative RKIP-regulated proteins in GIST, such as COL3A1.ConclusionsUsing a multidimensional integrative analysis, we identified, for the first time in GIST, molecules and pathways modulated by RKIP that may potentially drive metastasis and, consequently, poor prognosis in this disease.
PLGA nanoparticles engineering extracellular vesicles from human umbilical cord mesenchymal stem cells ameliorates polyethylene particles induced periprosthetic osteolysis
Authors: Xie Jie , Hu Yihe , Su Weiping , Chen Sijie , Wang Jiahao , Liang Shuailong , Chen Mingyu , Wang Haoyi , Ma Tianliang 
Published: October 2023

AbstractThe wear particle-induced dissolution of bone around implants is a significant pathological factor in aseptic loosening, and controlling prosthetic aseptic loosening holds crucial social significance. While human umbilical cord mesenchymal stem cell-derived exosomes (HucMSCs-Exos, Exos) have been found to effectively promote osteogenesis and angiogenesis, their role in periprosthetic osteolysis remains unexplored. To enhance their in vivo application, we engineered HucMSCs-Exos-encapsulated poly lactic-co-glycolic acid (PLGA) nanoparticles (PLGA-Exos). In our study, we demonstrate that PLGA-Exos stimulate osteogenic differentiation while inhibiting the generation of reactive oxygen species (ROS) and subsequent osteoclast differentiation in vitro. In vivo imaging revealed that PLGA-Exos released exosomes slowly and maintained a therapeutic concentration. Our in vivo experiments demonstrated that PLGA-Exos effectively suppressed osteolysis induced by polyethylene particles. These findings suggest that PLGA-Exos hold potential as a therapeutic approach for the prevention and treatment of periprosthetic osteolysis. Furthermore, they provide novel insights for the clinical management of osteolysis.
Insight into extracellular vesicles in vascular diseases: intercellular communication role and clinical application potential
Authors: Liu Wenxiu , Jin Meiqi , Chen Qiuyan , Li Qiaoyu , Xing Xiaoyan , Luo Yun , Sun Xiaobo 
Published: October 2023

Abstract
Background
Cells have been increasingly known to release extracellular vesicles (EVs) to the extracellular environment under physiological and pathological conditions. A plethora of studies have revealed that EVs contain cell-derived biomolecules and are found in circulation, thereby implicating them in molecular trafficking between cells. Furthermore, EVs have an effect on physiological function and disease development and serve as disease biomarkers.

Main body
Given the close association between EV circulation and vascular disease, this review aims to provide a brief introduction to EVs, with a specific focus on the EV cargoes participating in pathological mechanisms, diagnosis, engineering, and clinical potential, to highlight the emerging evidence suggesting promising targets in vascular diseases. Despite the expansion of research in this field, some noticeable limitations remain for clinical translational research.

Conclusion
This review makes a novel contribution to a summary of recent advances and a perspective on the future of EVs in vascular diseases.
Bilateral ovarian fibromas as the sole manifestation of Gorlin syndrome in a 22-year-old woman: a case report and literature review
Authors: Zhu Menghan , Li Jun , Duan Jie , Yang Jing , Gu Weiyong , Jiang Wei 
Published: October 2023

Abstract
Background
Nevoid basal cell carcinoma syndrome (NBCCS, Gorlin syndrome) is a rare autosomal dominantly inherited disorder that is characterized by multisystem disorder such as basal cell carcinomas, keratocystic odontogenic tumors and skeletal abnormalities. Bilateral and/or unilateral ovarian fibromas have been reported in individuals diagnosed with NBCCS.

Case presentation
A 22-year-old female, presented with low back pain, and was found to have bilateral giant adnexal masses on pelvic ultrasonography, which had been suspected to be malignant ovarian tumors. Positron emission tomography/computed tomography showed multiple intracranial calcification and skeletal abnormalities. The left adnexa and right ovarian tumor were resected with laparotomy, and pathology revealed bilateral ovarian fibromas with marked calcification. We recommended the patient to receive genetic testing and dermatological examination. No skin lesion was detected. Germline testing identified pathogenic heterozygous mutation in PTCH1 (Patched1).

Conclusions
The possibility of NBCCS needs to be considered in patients with ovarian fibromas diagnosed in an early age. Skin lesions are not necessary for the diagnosis of NBCCS. Ovarian fibromas are managed with surgical excision with an attempt at preserving ovarian function. Follow-up regime and counseling on options for future fertility should be offered to patients.
Results of the transition from posterolateral to anterior minimally invasive approach for total hip arthroplasty
Authors: Obando Daniel Vernaza , Gallego Kelly Johana , Gonzalez Sofía , Álvarez Alejandro Gallego , Bautista María , Sánchez-Vergel Alfredo 
Published: October 2023

Abstract
Background
The anterior minimally invasive (AMI) approach reduces soft tissue damage, risk of dislocation and enhances recovery, but it is associated with certain complications. The aim of this study is to compare the outcomes of patients who underwent total hip arthroplasty (THA) through posterolateral (PL) and AMI approaches performed by the same surgeon, in order to determine the learning curve associated with this new approach.

Methods
This retrospective cohort study included patients who underwent THA via PL and AMI approach between 2017 and 2022, with a minimum follow-up of 1 year. Hip fracture and oncologic patients were excluded. Demographic variables, functional scores and perioperative complications were assessed. A bivariate analysis was performed to identify differences between groups.

Results
Data of 124 AMI and 120 PL patients were analyzed. Demographic characteristics among groups were homogeneous. Functional outcomes at 3 months were superior for AMI (Oxford: 43 vs. 38; p < 0.05), no dislocations were identified (0% vs. 4.2%; p < 0.05) and no differences in the transfusion rate were found (6.5% AMI vs. 6.7% PL; p = 0.996). Infection rate was 4% for AMI and 3.4% for PL (p = 0.572). Surgical time was shorter for the PL approach, but the median surgical time of the last 25 AMI cases was shorter.

Conclusions
The AMI approach is an excellent alternative for patients requiring THA. Although surgical time and perioperative bleeding were greater during the learning curve, this approach offers improved functional outcomes and a lower dislocation rate, without significant differences in transfusion and infection outcomes, demonstrating that responsible innovation and safe implementation of new techniques is possible.
Continuous data capture of gait and mobility metrics using wearable devices for postoperative monitoring in common elective orthopaedic procedures of the hip, knee, and spine: a scoping review
Authors: Natarajan Pragadesh , Fonseka R. Dineth , Maharaj Monish Movin , Koinis Lianne , Mobbs Ralph Jasper 
Published: October 2023

Abstract
Background
Surgical procedures involving the hip, knee, or spine represent a majority of orthopaedic procedures performed electively in the health care system. Postoperative care is a key aspect of surgery and mobilisation without injury is the primary objective. Recent advances in wearable technologies allow objective evaluation of walking metrics to inform and guide postoperative care following orthopaedic surgery.

Purpose
The aim of this scoping review is to explore current applications of wearable devices, objective data capture and gait analysis in monitoring postoperative recovery following commonly performed elective orthopaedic procedures of the hip, knee and spine.

Methods
A search against pre-defined criteria was performed on the following scientific databases from date of inception to February 28th, 2021: Medline (via OvidSP), Embase (via OvidSP) and Cochrane Library (via CENTRAL). Data were collected according to a predetermined checklist including study participants, surgery, wearable device (model), sensor location, and monitoring parameters such as mobility metrics, monitoring timepoints and monitoring duration for each study included in our review. Quality was assessed independently using the Newcastle Ottawa Scale (NOS).

Conclusions
To our knowledge, this is the first review of wearable monitoring (of postoperative recovery) following hip, knee and spine surgery. Patients undergoing elective orthopaedic procedures may benefit from wearable monitoring of their walking health and mobility metrics.
Robustness of ad hoc microphone clustering using speaker embeddings: evaluation under realistic and challenging scenarios
Authors: Kindt Stijn , Thienpondt Jenthe , Becker Luca , Madhu Nilesh 
Published: October 2023

AbstractSpeaker embeddings, from the ECAPA-TDNN speaker verification network, were recently introduced as features for the task of clustering microphones in ad hoc arrays. Our previous work demonstrated that, in comparison to signal-based Mod-MFCC features, using speaker embeddings yielded a more robust and logical clustering of the microphones around the sources of interest. This work aims to further establish speaker embeddings as a robust feature for ad hoc microphone clustering by addressing open and additional questions of practical interest, arising from our prior work. Specifically, whereas our initial work made use of simulated data based on shoe-box acoustics models, we now present a more thorough analysis in more realistic settings. Furthermore, we investigate additional important considerations such as the choice of the distance metric used in the fuzzy C-means clustering; the minimal time range across which data need to be aggregated to obtain robust clusters; and the performance of the features in increasingly more challenging situations, and with multiple speakers. We also contrast the results on the basis of several metrics for quantifying the quality of such ad hoc clusters. Results indicate that the speaker embeddings are robust to short inference times, and deliver logical and useful clusters, even when the sources are very close to each other.
Structural characterization, physicochemical property, and antioxidant activity of polysaccharide components from Eucommia ulmoides leaves
Authors: Liu Mengpei , Wang Yan , Wang Rong , Du Qingxin , Wang Lu 
Published: October 2023

Abstract
Background
Eucommia ulmoides (E. ulmoides) leaves are identified as a new resource of medicine and food homology. In this study, the structural characterization, physicochemical properties, and antioxidant activity of E. ulmoides polysaccharides (EUP) were studied.

Results
Three components with different molecular weights of 1.51 × 105 Da (EUP1), 3.05 × 104 Da (EUP2) and 1.17 × 105 Da (EUP3) were purified from E. ulmoides leaves. They were composed of l-rhamnose (Rha), d-arabinose (Ara), d-mannose (Man), d-glucose (Glu) and d-galactose (Gal), while EUP2 also contained small amounts of d-xylose (Xyl). Three components all had typical polysaccharides absorption peaks, which may be polysaccharides with β configuration of pyranose structure, and amorphous structure of acid polysaccharides with good thermal stability below 270 ℃. However, the molecular weight, monosaccharide composition and apparent morphology of the three components were different, resulting in a stronger scavenging ability of EUP2 and EUP3 against DPPH and OH free radicals.

Conclusion
The results will provide a theoretical reference for developing EUP-related foods and drugs.

Graphical Abstract
Attribute Selection Hybrid Network Model for risk factors analysis of postpartum depression using Social media
Authors: Gopalakrishnan Abinaya , Gururajan Raj , Venkataraman Revathi , Zhou Xujuan , Ching Ka Chan , Saravanan Arul , Sen Maitrayee 
Published: October 2023

Abstract
Background and objective
Postpartum Depression (PPD) is a frequently ignored birth-related consequence. Social network analysis can be used to address this issue because social media network serves as a platform for their users to communicate with their friends and share their opinions, photos, and videos, which reflect their moods, feelings, and sentiments. In this work, the depression of delivered mothers is identified using the PPD score and segregated into control and depressed groups. Recently, to detect depression, deep learning methods have played a vital role. However, these methods still do not clarify why some people have been identified as depressed.

Methods
We have developed Attribute Selection Hybrid Network (ASHN) to detect the postpartum depression diagnoses framework. Later analysis of the post of mothers who have been confirmed with the score calculated by the experts of the field using physiological questionnaire score. The model works on the analysis of the attributes of the negative Facebook posts for Depressed user Diagnosis, which is a large general forum. This framework explains the process of analyzing posts containing Sentiment, depressive symptoms, and reflective thinking and suggests psycho-linguistic and stylistic attributes of depression in posts.

Results
The experimental results show that ASHN works well and is easy to understand. Here, four attribute networks based on psychological studies were used to analyze the different parts of posts by depressed users. The results of the experiments show the extraction of psycho-linguistic markers-based attributes, the recording of assessment metrics including Precision, Recall and F1 score and visualization of those attributes were used title-wise as well as words wise and compared with daily life, depression and postpartum depressed people using Word cloud. Furthermore, a comparison to a reference with Baseline and ASHN model was carried out.

Conclusions
Attribute Selection Hybrid Network (ASHN) mimics the importance of attributes in social media posts to predict depressed mothers. Those mothers were anticipated to be depressed by answering a questionnaire designed by domain experts with prior knowledge of depression. This work will help researchers look at social media posts to find useful evidence for other depressive symptoms.
Parenting by mothers in immigrant families from Poland, Russia and Turkey in Germany: Migration-related similarities or origin-related differences?
Authors: Öztürk Yasmin , Reisenauer Eveline , Castiglioni Laura , Walper Sabine 
Published: October 2023

AbstractEven if families in migration contexts have been the subject of an increasing amount of attention in migration research in the recent years, there is a noticeable knowledge deficit with regard to current parenting practices and socialization goals of immigrant families living in Germany. This is particularly striking since child-rearing is of central importance for children’s development and their educational pathways. This paper seeks to fill this gap, drawing on the survey “Growing up in Germany: Everyday Life” (AID:A) provided by the German Youth Institute (DJI). Similarities and differences in attitudes towards parenting among immigrant mothers compared to mothers without migration backgrounds were analyzed with regard to parenting practices (emotional warmth, punishment and child’s active participation) and socialization goals (performance/self-control and positive social behavior). In a sample of 5870 mothers reporting on a child under the age of nine, mothers in families where both they and the father had Turkey, Russia or Poland as their country of origin were included and compared to autochthonic families. The results revealed significant differences in parenting practices and socialization goals between mothers with and without migration backgrounds. Concerning parenting practices, Turkish and Russian mothers differed significantly from German mothers with regard to emotional warmth and punishment. In terms of socialization goals, all immigrant mothers placed more emphasis on both performance/self-control, as well as positive social behavior for their children than mothers without migration backgrounds in Germany.
Translation to German and linguistic validation of the Rapid Assessment of Physical Activity (RAPA) questionnaire
Authors: Kulnik Stefan Tino , Gutenberg Johanna , Mühlhauser Kathrin , Topolski Tari , Crutzen Rik 
Published: October 2023

Abstract
Purpose
To produce a culturally adapted translation of the Rapid Assessment of Physical Activity (RAPA) questionnaire for German speaking Austrians and to conduct a linguistic validation of the new language version.

Methods
The original English RAPA questionnaire was translated into German for Austria and underwent an independent forward and back translation, followed by cognitive debriefing interviews with older adults aged 55 to 78 years with and without health conditions (n = 13), for linguistic validation.

Results
Several distinct choices were made in the translation of the RAPA questionnaire to German, including the use of colloquial terms for ‘physical activity’ and ‘intensity’; and the decision to keep to the original examples and images of different physical activities for illustrating the intensity levels (light, moderate, vigorous) of physical activity. In cognitive debriefing, interviewees commented that some example activities for the respective intensity levels could – depending on the individual – also represent a higher or lower intensity level; and that the wording of RAPA items 4 and 5, which describe the category ‘under-active regular’ aerobic activity, was difficult to understand. Both issues were addressed and resolved through minor iterative modifications made during the cognitive debriefing process.

Conclusions
A new version of the RAPA questionnaire in German for Austria has been produced by forward and back translation and linguistic validation. The questionnaire may now undergo psychometric evaluation.
Efficacy and safety of baricitinib in treatment of systemic lupus erythematosus: a systematic review and meta-analysis
Authors: Allam Abdallah R. , Alhateem Mohamed Salah , Mahmoud Abdelrahman Mohamed 
Published: October 2023

Abstract
Background
SLE is an autoimmune disease marked by broad immunological dysregulation and multi-system inflammation. Baricitinib is one of the novel treatments for SLE. We conducted this meta-analysis to evaluate its safety and effectiveness in treating SLE.

Method
We looked for all published randomized controlled trials in PubMed, Scopus, Web of Science, and Cochrane and included all RCTs comparing baricitinib and placebo in the treatment of SLE. Review Manager 5.4 program was used for data analysis.

Results
Three trials with a total of 1849 individuals were included. Participants in the baricitinib group were significantly more likely to attain SRI-4 response than those in the placebo group [RR = 1.11, 95% CI (1.02, 1.21), P = 0.01]. Additionally, baricitinib performed better than the placebo in terms of reduction of ≥ 4 points from baseline in SLEDAI-2 K score [RR = 1.13, 95% CI (1.04, 1.22), P = 0.004]. In terms of SLEDAI-2 K remission of arthritis or rash, baricitinib was also superior to placebo [RR = 1.08, 95% CI (1.00, 1.17), P = 0.04]. Treatment-emergent adverse events did not differ significantly [RR = 1.01, 95% CI (0.97, 1.05), P = 0.61].

Conclusion
Baricitinib is potentially safe and effective in the treatment of SLE. It has successfully met the study’s primary endpoint and some secondary endpoints highlighting its potential to improve the outcomes of SLE. Despite achieving an SRI-4 response, glucocorticoids sparing and some other secondary outcomes weren’t reached by baricitinib.
Effectiveness of integrated education and relaxation program on migraine-related disability: a randomized controlled trial
Authors: Hisham Sara , Manzour Ayat , Fouad Mohammed M. , Amin Randa M. , Hatata Hisham A. , Marzouk Diaa 
Published: October 2023

Abstract
Background
Migraine is one of the most disabling disorders worldwide. Globally, in 2019, headache disorders were the cause of 46.6 million years of disability, with migraine accounting for 88.2% of these. The value of integrative strategies in migraine management has been raised due to the recurrent and provoked nature of migraine. So, the current study aimed to evaluate the effectiveness of implementing a therapeutic patient education and relaxation training program versus usual pharmacological treatment alone on the frequency, severity, and duration of migraine attacks as the primary outcome and migraine-related disability and quality of life as the secondary outcome. A randomized controlled trial was conducted at the specialized headache clinic of a tertiary referral center. Sixty patients were randomly assigned to intervention or control groups. Participants in the intervention arm received the education and relaxation training program and were instructed to perform daily relaxation exercises in addition to their routine pharmacological treatment, whereas the control group only received their routine treatments. Follow-up was done after 1 and 3 months using a headache diary and a migraine-specific quality of life questionnaire (MSQ).

Result
After implementation of the program, there was a significant reduction in migraine attack severity in the intervention group compared to the control group, and they also had significantly fewer migraine headache days/month and duration of migraine attacks compared to patients in the control group. Statistically significant improvement in the role-function restrictive, role-function preventive, and emotional function domains of MSQ.

Conclusion
An integrated migraine management program has a significant effect on reducing the burden of migraine attacks and improving the daily activities of migraine sufferers.