bluuebunny/crossref_metadata_2025_split huggingface dataset:
Statistical crystallography reveals an allosteric network in SARS-CoV-2 Mpro
Authors: Creon Anne , Scheer T. Emilie S. , Reinke Patrick , Mashhour Aida Rahmani , Günther Sebastian , Niebling Stephan , Schamoni-Kast Kira , Uetrecht Charlotte , Meents Alke , Chapman Henry N. , Sprenger Janina , Lane Thomas J. 
Published: January 2025

AbstractTo interpret and transmit biological signals, proteins use correlated motions. Experimental determination of these dynamics with atomic resolution remains a key challenge. Here, using thousands of crystals of the main protease (Mpro) from SARS-CoV-2, we were able to infer a model of the protein’s correlated motions. Mprois regulated by concentration, becoming enzymatically active after forming a homodimer. To understand the correlated motions that enable dimerization to activate catalysis, we employed our model, predicting which regions of the dimerization domain are structurally linked to the active site. Mutations at these positions, expected to disrupt catalysis, resulted in a dramatic reduction in activity in one case, a mild effect in the second, and none in the third. Additional crystallography and biophysical experiments provide a mechanistic explanation for these results. Our work suggests that a statistical crystallography can determine protein correlated motions and rationalize their biological function.TeaserCrystallography at scale goes beyond a single structure, revealing native-state protein dynamics.
Experimental assessment of 3D-printed traps and chemical attractants for the collection of wildDrosophila melanogaster
Authors: Keene-Snickers Alexandra H. , Dunham Tillie J. , Stenglein Mark D. 
Published: January 2025

ABSTRACTDrosophila melanogaster, the common fruit fly, has been instrumental to our understanding of evolution, genetics and disease. There are benefits to studying these flies in the wild, including assessment of their naturally occurring microbiota. To facilitate efforts to catch wildD. melanogaster, we designed two fly traps and evaluated several candidate attractants. The first trap utilized a stable food substrate that can be used to catch live flies to establish new lab colonies. The second trap was designed to be reusable and easy to ship to enable the collection of flies over time from diverse locations. We evaluated several chemical attractants derived from banana and from marula fruit, which is the proposed ancestral food host ofD. melanogaster. We found that wild flies were preferentially attracted to banana-based odorants over marula-derived ones. Overall, these traps and attractants represent an inexpensive and simple option for the collection of wildD. melanogasterand related species for sampling or colony establishment.
Multiplexing of visual-auditory signals in a predominantly auditory brain region
Authors: Schmehl Meredith N. , Chen Yunran , Tokdar Surya T. , Groh Jennifer M. 
Published: January 2025

AbstractA recently proposed theory of neural representation postulates that when more than one stimulus is to be encoded, neurons in that representation may fluctuate between encoding each item. To date, such work has considered only cases in which both stimuli are of the same sensory modality, such as how two sounds are encoded in an auditory brain region. Here, we consider the influence of sensory modality on this process, asking whether stimuli of a non-dominant sensory modality can evoke or influence the fluctuating activity patterns observed for combinations of stimuli of a dominant sensory modality. Specifically, we investigated the impact of combining visual and auditory stimuli on single-unit activity in the inferior colliculus, a predominantly auditory structure, in monkeys performing a behavioral task involving reporting all stimulus locations in a given trial. We found that fluctuating activity is evident when a single sound and a single visual stimulus are paired at the same spatial location, indicating that fluctuating activity can be induced when one item is not from a brain area’s preferred modality, and even when only a single spatial location is involved and spatial attention is directed to only one place. Fluctuating activity was also identified when visual stimuli were paired with sounds presented at two spatial locations. The IC appeared to maintain a representation of all stimuli, regardless of the sequence in which the monkey localized the targets (although in one monkey there was a bias toward greater representation of the second reported location). Together, these results indicate that fluctuating activity occurs in response to multimodal stimuli in a predominantly unimodal brain structure and is geared toward preservation of all sensory information rather than attentional filtering.Impact StatementWe found that when visual stimuli are paired with sounds, they can induce fluctuating activity in neurons that are primarily sensitive to sound alone. Such activity fluctuations may permit the representation of both the visual and auditory stimuli in an overlapping population of neurons.
A modular architecture for trial-by-trial learning of redundant muscle activity patterns in novel sensorimotor tasks
Authors: Rebelo Dal’Bello Lucas , Berger Denise Jennifer , Borzelli Daniele , Burdet Etienne , d’Avella Andrea 
Published: January 2025

AbstractThe coordination of the multiple degrees-of-freedom of the human body may be simplified by muscle synergies, motor modules which can be flexibly combined to achieve various goals. Studies investigating adaptation to novel relationships between muscle activity and task outcomes found that altering the recruitment of such modules is faster than the learning of their structuresde novo. However, how learning new synergy recruitments or new synergy structures may occur remains unclear. While trial-by-trial learning of novel sensorimotor tasks has been successfully modeled at the level of task variables, few models accounted for the redundancy of the motor system, particularly at the muscular level. However, these models either did not consider a modular architecture of the motor system, or assumeda prioriknowledge of the sensorimotor task. Here, we present a computational model for the generation of redundant muscle activity where explicitly defined modules, implemented as spatial muscle synergies, can be updated together with their recruitment coefficients through an error-based learning process dependent on a forward model of the sensorimotor task, which is not assumed to be known a priori. Our model can qualitatively reproduce the experimental observations of slower learning and larger changes in the structure of the muscle activity under sensorimotor tasks that require the learning of novel patterns of muscle activity, providing further insights into the modular organization of the human motor system.Author summaryIt has been proposed that muscles are recruited in modules, called muscle synergies, rather than on a muscle-by-muscle basis. This modular organization was shown to affect the learning of novel tasks, where simulated remapping of the forces generated by the muscles (“virtual surgeries”) that make the synergies ineffective are more difficult to learn. Previous models of trial-by-trial learning in a modular architecture have assumed prior knowledge of the motor task (in particular, how to modify the muscles / synergies recruitment given an error), which might not be the case for tasks in which novel patterns of muscle activity are required. In contrast, models which assumed no prior knowledge of the task have not investigated the role of modularity. Here, we propose a computational model of the trial-by-trial adaptation of muscle synergies, their recruitment, and the concurrent learning of the internal model of the musculoskeletal system responsible for error correction. Our results show that this model replicates experimental observations of slower learning and larger changes in the structure of the muscle activity in sensorimotor tasks that require the learning of novel patterns of muscle activity, providing insight into learning-related changes of muscle activity in novel sensorimotor tasks.
Anterior cingulate cortex in complex associative learning: monitoring action state and action content
Authors: Huang Wenqiang , Hall Arron F , Kawalec Natalia , Opalka Ashley N , Liu Jun , Wang Dong V 
Published: January 2025

AbstractEnvironmental changes necessitate adaptive responses, and thus the ability to monitor one’s actions and their connection to specific cues and outcomes is crucial for survival. The anterior cingulate cortex (ACC) is implicated in these processes, yet its precise role in action monitoring and outcome evaluation remains unclear. To investigate this, we developed a novel discrimination–avoidance task for mice, designed with clear temporal separation between actions and outcomes. Our findings show that ACC neurons primarily encode post-action variables over extended periods, reflecting the animal’s preceding actions rather than the outcomes or values of those actions. Specifically, we identified two distinct subpopulations of ACC neurons: one encoding the action state (whether an action was taken) and the other encoding the action content (which action was taken). Importantly, increased post-action ACC activity was associated with better performance in subsequent trials. These findings suggest that the ACC supports complex associative learning through extended signaling of rich action-relevant information, thereby bridging cue, action, and outcome associations.
Mathematical modelling of tissue growth control by a negative feedback
Authors: Kazmierczak B. , Volpert V. 
Published: January 2025

AbstractThis study investigates the regulation of tissue growth through mathematical modeling of systemic and local feedback mechanisms. Employing reaction-diffusion equations, the models explore the dynamics of tissue growth, emphasizing endocrine signaling and inter-tissue communication. The analysis identifies critical factors influencing the emergence of spatial structures, bifurcation phenomena, the existence and stability of stationary pulse and wave solutions. It also elucidates mechanisms for achieving coordinated tissue growth. In particular, if negative feedback is sufficiently strong, their final finite size is provided by a stable pulse, otherwise they manifest unlimited growth in the form of a wave. These findings contribute to the theoretical insights into biological processes such as embryogenesis, regeneration, and tumor development, while highlighting the role of feedback systems in maintaining physiological homeostasis.
Identification of M4muscarinic acetylcholine receptor expressing astrocytes that regulate locomotion and survival in murine prion disease
Authors: Tejeda Gonzalo S. , Molloy Collin , Jones Carrie K. , Lindsley Craig W. , Milligan Graeme , Tobin Andrew B. 
Published: January 2025

AbstractWhilst there has been much focus on the function of neuronally expressed members of the muscarinic acetylcholine receptor family (mAChR) less attention has been paid to the expression profile and role of the five members of this family (M1-M5mAChRs) in non-neuronal cells of the brain. Using genetically engineered mice we identify a previously unappreciated sub-population of astrocytes expressing the M4mAChR subtype. These are located in various brain regions that include the brainstem, hypothalamus and, most abundantly, the cerebellum. Signalling and proteomic analysis of M4mAChR positive astrocytes from the cerebellum established the functional expression of this receptor subtype and its role in the regulation of protein expression. Genetic ablation of M4mAChR positive astrocytes in mice revealed a specific role in locomotion behaviour. Importantly, in the context of murine prion disease, a model of terminal neurodegeneration associated with profound neuroinflammation, we observed a significant expansion of M4mAChR positive astrocytes and report ablation experiments that established that this astrocyte sub-population has a detrimental effect on late-stage disease. Together we provide evidence of a sub-population of M4mAChR expressing astrocytes that play a specific role in normal neurophysiology and the progression of inflammatory neurodegenerative disease.
Unveiling the
CP
-odd Higgs boson in a generalized 2HDM at a muon collider
Authors: Das Nandini , Ghosh Nivedita 
Published: January 2025

We revisit the generalized two-Higgs-doublet model (2HDM) with a lepton specific (Type-X) Yukawa sector in context of the muon collider. The model offers a large region of parameter space where it can provide a solution to the observed muon (g−2) excess. Specifically the low mass CP odd scalar of this model plays an important role to fit this anomaly. Interestingly, the coupling of the pseudoscalar with the muons is proportional to tanβ (which equals to the ratio of vacuum expectation values of the Higgs doublets) which dominantly controls the one loop and two loop contributions of the muon magnetic moment, therefore it can be probed directly at a muon collider with greater sensitivity in comparison to the Large Hadron Collider. Primarily focusing on a parameter space where muon g−2 anomaly is satisfied, we explore the available theoretical and experimental constraints arising from vacuum stability, unitarity, lepton flavor violation, electroweak precision constraint, B-meson decay, and collider experiments on the model. In the parameter space where all these constraints are satisfied, we propose to study the production of the light pseudoscalar A in association with a photon at a 3 TeV muon collider. Subsequent decay of A into a τ pair will produce a striking l+l−γ plus missing energy signature.

Published by the American Physical Society
2025
Influence mechanisms of digital construction organizations’ capabilities on performance: evidence from SEM and fsQCA
Authors: Chen Yonghong , Qiu Wenyi , Xiao Mengxia 
Published: January 2025

PurposeWith the rise of digital construction, using organizational capabilities to improve project performance in a turbulent environment has become critical for the high-quality development of the construction industry. However, the complex relationships among them remain unclear. Therefore, this study explores these linear relationships under the digital construction mode and reveals the driving mechanism of multi-factor linkage on project performance.Design/methodology/approachData were collected from 263 project participants in digital construction projects in China using a questionnaire. Hypothesis testing was conducted using partial least square structural equation modeling, and the differentiated patterns of project performance formation were revealed through fuzzy-set qualitative comparative analysis.FindingsOrganizational information technology, innovation, coordination, integration management and emergency management capabilities improve project performance. Environmental turbulence is a positive moderator between coordination capabilities and project performance, while other capabilities do not align with environmental turbulence. The research obtained five equivalent configurations for achieving high project performance, such as “capability layout” and “internal driven,” and two paths that lead to non-high project performance. Finally, in contrast to existing studies, we discovered the outstanding contribution of emergency management capabilities to project performance and the auxiliary effect of information technology capabilities.Originality/valueThis study innovatively integrates a dimensional framework of construction project organizations’ capabilities under a digital construction mode and extends the organizational capabilities to the specific and operational capability dimension level. Furthermore, this study opens the “black box” of the influence of organizational capabilities on project performance in environmental turbulence and reveals the differentiated and equivalent configurations for the formation of project performance. The study broadens the theoretical perspective of organizational capabilities on project performance research in the digital context and provides practical enlightenment for guiding the capability configuration of construction project organizations in a turbulent environment. The study broadens the theoretical perspective of organizational capabilities on project performance research in the digital context and provides practical enlightenment for guiding the capability configuration of construction project organizations in a turbulent environment.
Assessment of the challenges of integrating cyber-physical systems in Nigerian construction
Authors: Oke Ayodeji Emmanuel , Adekunye Olusegun Johnson , Adeosun Femi Emmanuel , Aliu John , Akanni Prince Oluleye , Aghimien Douglas , Aigbavboa Clinton Ohis 
Published: January 2025

PurposeThe internet’s revolutionary impact on communication, information access and business transactions exemplifies how technology can reshape human behaviour. Similarly, cyber-physical systems (CPSs) hold immense potential to transform our interaction and control of the physical environment. This study investigates the challenges hindering the adoption of CPSs in Nigeria’s construction industry, aiming to pave the way for a more sustainable future in construction.Design/methodology/approachThis study examined challenges related to CPSs in Nigerian sustainable construction projects. Data were gathered through a literature review and a questionnaire survey of Lagos-based construction professionals (architects, quantity surveyors, engineers and builders). Statistical methods were used to analyse the data. Mean and standard deviation determined respondents’ opinions’ central tendency and variability. The data’s suitability for factor analysis was confirmed through Kaiser–Meyer–Olkin (KMO) and Bartlett’s sphericity tests, indicating strong correlations between variables. Factor analysis subsequently identified key challenges hindering CPSs implementation in Nigerian construction.FindingsThe mean and standard deviation analysis findings indicated that the most significant challenges of CPSs for sustainable construction projects in Nigeria are lack of awareness, inadequate public–private partnership, professional complacencies, data overload and lack of capital. In addition, the analysis was also conducted using factor analysis, which includes five groupings: financial inhibition, management barrier, geopolitical inhibition, national security and lack of awareness.Originality/valueThis study recommended that the challenges listed in this study should be considered and worked on through financial, political and professional support of CPSs for sustainable construction projects, such as Sustainable Development Goals (SDGs) programmes and national awareness programmes.
The moderating role of perceived trust in predicting the adoption intention of consumers toward online pharmacies using the application of UTAUT model
Authors: Saraswat Sanjeev , Singh Pankaj 
Published: January 2025

Purpose
This study aims to identify the factors influencing consumer perception and adoption intention toward online pharmacy.

Design/methodology/approach
A questionnaire was formulated based on the unified theory of acceptance and use of technology (UTAUT) framework scales available in the existing literature. An aggregate of 451 respondents participated in the survey. To test the formulated hypothesis, confirmatory factor analysis and structure equation modeling are used for analyzing the structural relationships between dependent and independent variables.

Findings
This analysis inspects the significant effect of perceived trust, consumer perception and different UTAUT framework constructs on the adoption intention of online pharmacy consumers. The results determine the substantial moderating effect of perceived trust between adoption intention and consumers’ perception of online pharmacies.

Research limitations/implications
This research extended the application of the UTAUT model with the adoption intention of online pharmacy consumers. Only specific constructs have been selected from the UTAUT model in this study.

Practical implications
The study outcomes are useful to pharmacy managers to realize the role of technological platforms in facilitating the online selling of medicines to pharmacy consumers.

Social implications
The findings are helpful for pharmaceutical companies to improve their existing online buying process for pharmacy consumers seeking purchase of medicines through online pharmacies.

Originality/value
This analysis is unique and contains newness in the form of the moderating role of perceived trust between constructs of UTAUT and customer adoption of online pharmacies.
New experiences in the medical industry: online-to-offline (O2O) commerce usage intentions
Authors: Chin Chee Hua , Wong Jacqueline Wei-Chi , Chan Wei Chiang 
Published: January 2025

Purpose
This study aims to propose a research framework grounded in the technology acceptance model (TAM) to assess the new service experiences in the medical industry through examining the intention to use online-to-offline (O2O) medical platforms. It focuses on the connections between immediacy of connection, context-based affordability, reliability and perceived convenience and perceived usefulness. This study also looks at how these determinants affect users’ intentions to use O2O medical platforms, with a particular emphasis on the moderating effect of customer online reviews.

Design/methodology/approach
This study was a quantitative research endeavour grounded in the TAM model that analysed usage intention of O2O medical platforms. Conducted in Sarawak, within the Borneo region, a total of 251 rows of data were collected by questionnaire and underwent initial analysis using SPSS, followed by a more in-depth two-stage partial least squares structural equation model analysis conducted with SmartPLS4.

Findings
According to this study’s findings, perceived usefulness and perceived convenience are significantly impacted by the proposed predictors – immediacy of connection, context-based affordability and reliability. Furthermore, intention to use O2O medical platforms was discovered to have a positive and significant impact from perceived usefulness and perceived convenience. It is noteworthy that the association among perceived convenience and perceived usefulness – which leads to usage intention of O2O medical platforms – did not show the moderating effect of customer online reviews.

Research limitations/implications
The findings suggested that context-based affordability, immediacy of connection and reliability need to be focused on by the information and communication technology developers, policymakers and medical professionals, as these variables can influence perceived convenience and usefulness, which will further impact the intention to use O2O medical platforms.

Originality/value
The formulated research framework holds significance in comprehending the usage intention of O2O medical platforms among consumers in Sarawak. O2O medical platforms have experienced global growth; however, limited studies were found in the Malaysian context. Hence, the objective of this study is to examine the intention to use O2O medical platforms in a relatively understudied area, specifically in Sarawak, an island located in Borneo, Malaysia.