bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete genome sequence of a novel mitovirus isolated from the fungus Fusarium oxysporum f. sp. ginseng causing ginseng root rot
Authors: Ma Kai Ge , Cai Li Ping , Wang Ruo Jing , Wang Jun , Zhan Hao Xin , Ni He Chi , Lu Bao Hui , Zhang Yan Jing , gao jie 
Published: November 2023

Abstract
A novel Mitoviridaevirus, “Fusarium oxysporum mitovirus 2” (FoMV2), was isolated from the pathogenic Fusarium oxysporum f. sp. ginseng strain 0414 infesting Panax ginseng. The FoMV2 genome was 2388 nt in length and 30.57% in GC content. It carried an ORF encoding a 713-amino-acid RNA-dependent RNA polymerase (RdRp) with a molecular weight of 83.05 kDa. The homology between FoMV2 and the viruses isolated from “Botrytis cinerea mitovirus 8” and “Fusarium verticillioides mitovirus 1” was 87.94% and 77.85%, respectively. Phylogenetic analysis showed that FoMV2 belonged to Mitoviridae. To our knowledge, this is the first mycovirus isolated from F. oxysporum by ginseng root rot.
Rosuvastatin-laden Nanofibrous Patches for Effective Treatment of Corneal Ulcer in Experimental Animals
Authors: Helmy Abdelrahman M. , Hamed Asmaa A. , Abdelghany Ahmed A. , Hafez Sara Mohamed Naguib Abdel , Rouby Waleed M.A. El , Aly Usama F. 
Published: November 2023

Abstract
Corneal ulceration is a "silent epidemic" that impacts laborers’ lives, particularly in low-income countries, and calls for a unique ophthalmic drug delivery strategy that overcomes ocular barriers to promptly achieve therapeutic outcomes while remaining cost-effective. Rosuvastatin, a third-generation statin that significantly accelerates tissue regeneration and wound healing, could be proposed as a potential treatment for corneal ulcers. Based on these pillars, the current study aims to develop a nanofibers-based corneal patch laden with rosuvastatin, for the first time, as a novel approach for the treatment of corneal ulcers. Several characterizations were performed for the rosuvastatin-laden nanofibers (e.g., drug encapsulation efficiency, drug loading capacity, morphological analysis, elemental mapping, compatibility with other ingredients, and in vitro release studies) indicating the nanofibers' feasibility for the stated purpose. The therapeutic effects of rosuvastatin-laden nanofibers were examined on a surgically generated corneal ulcer rabbit model. The treatment outcomes were evaluated both macroscopically (e.g., corneal opacity degree and area of ulceration) and histologically demonstrating that the rosuvastatin-laden nanofibers heal corneal ulcers promptly. In light of this, the study emphasizes that rosuvastatin has a significant potential for healing corneal ulcers and that fast-release nanofibers may be an ideal drug delivery platform for treating corneal diseases topically.
Post-COVID-19 Retinal Manifestations in Pediatric Patient with Retinoblastoma: A Case Report
Authors: Ng Wei Loon , Tungsattayathitthan Usanee , Trinavarat Adisak , Atchaneeyasakul La-ongsri 
Published: November 2023

Abstract

Background
The coronavirus disease 2019 (COVID-19) has been associated with various ocular manifestations, with limited literature on posterior segment findings in pediatric cases, especially in the context of retinoblastoma (RB). We present a unique case of a pediatric patient with unilateral RB who developed posterior uveitis and retinal vasculitis in the fellow eye following a COVID-19 infection.
Case presentation
A one-year and four-month-old boy with unilateral RB developed small retinal infiltrates and retinal vasculitis in the fellow eye at six months after a COVID-19 infection. Laboratory work-up did not identify infectious or specific autoimmune disorders. Given the critical nature of retinoblastoma, it raised concerns about a tumor-related cause in the fellow eye. Ancillary investigations, including fundus fluorescein angiography, were performed to differentiate RB-related changes. However, the results did not align with the typical features of RB. Spontaneous resolution of retinal lesions and retinal vasculitis was observed six weeks later.
Conclusions
Pediatric patients with posterior uveitis or retinal vasculitis post-COVID-19 should undergo comprehensive diagnostic workup, akin to adults, to exclude alternative causes before attributing it to SARS-CoV-2-induced immune responses. Notably, in unique cases like RB, a thorough evaluation of tumor-related cause is imperative.
Descriptive sensory analysis and evaluation of lipid oxidation in mortadella developed with curcumin microcrystals and cochineal carmine
Authors: Henrique Jacqueline Thomé , Biason Maria Victória , Mendes Poliana dos Santos , Cardoso Flávia Aparecida Reitz , Leimann Fernanda Vitória , Gonçalves Odinei Hess , Bona Evandro , de Oliveira Anielle , Marques Leila Larisa Medeiros , Fuchs Renata Hernandez Barros , Droval Adriana Aparecida 
Published: November 2023

Abstract

The effects of adding cochineal carmine and annatto dyes in five mortadella formulations made with curcumin microcrystals were compared, evaluating the preference and describing them sensorially. Based on the optimized formulation obtained with color parameters, two formulations were elaborated, both added curcumin microcrystals and cochineal carmine. During 60 days pH, objective color, water retention capacity, lipid oxidation, and texture profile analyses were performed. The results demonstrate the possibility of excluding sodium erythorbate from formulations containing curcumin microcrystals. There was no significant difference in lipid oxidation between the samples, presenting at the end of 60 days a value of 0.11 mg and 0.10 mg of MDA kg− 1 for the two samples, respectively. There were also no relevant significant differences between for the two samples, nor between the evaluated storage times, and the average values obtained for pH, CRA, objective color, and TPA were expected for this type of cooked meat sausage. In the presence of curcumin microcrystals, the synthetic antioxidant, sodium erythorbate, can be eliminated from the formulations, as it does not affect the physical-chemical parameters studied, such as pH, water retention capacity, color objective, and texture profile.
A Novel Electronics Control Unit for Early Detection of Vehicle
Authors: Arafa Osama , Mansour Ahmed , Abdallah Mohamed , Omar Mohamed , Syam Fathy 
Published: November 2023

Abstract
Internal Combustion Engines (ICE) gets overheated due to several unavoidable reasons. The consequences of missing a timely shutdown of the ICE can be serious and the engine can be considerably injured. In this paper, a novel, efficient and reliable electronic control unit for providing ICE Overheating Early Detection and Alarm (OTEDA) is proposed, implemented and tested. Regular sampling of the engine block temperature and the rate of temperature change within a cooling system stressing period is used to establish an engine’s thermal signature. The theory of early detection based on the engine thermal signature is explained. The OTEDA hardware is built around the latest temperature sensing digital technology provided by Texas Instruments. The main OTEDA unit building blocks and the software structure are explained. Field test on several commercial vehicles of different make and brands proves the value added by the proposed Electronic Control Unit (ECU) in protecting the engine from incidental overheating.
Identification of m6A/m5C-related lncRNA signature for prediction of prognosis and immunotherapy efficacy in esophageal squamous cell carcinoma
Authors: Wang Jian , Xu Chao , Yu Bo , Cai Yiling , Wang Jianlin 
Published: November 2023

Abstract
Background
N6-methyladenosine (m6A) and 5-methylcytosine (m5C) RNA modifications have garnered significant attention in the field of epigenetic research due to their close association with human cancers. This study we focus on elucidating the expression patterns of m6A/m5C-related long non-coding RNAs (lncRNAs) in esophageal squamous cell carcinoma (ESCC) and assessing their prognostic significance and therapeutic potential.
Methods
Transcriptomic profiles of ESCC were derived from public resources. m6A/m5C-related lncRNAs were obtained from TCGA using Spearman’s correlations analysis. The m6A/m5C-lncRNAs prognostic signature was selected to construct a RiskScore model for survival prediction, and their correlation with the immune microenvironment and immunotherapy response was analyzed.
Results
A total of 606 m6A/m5C-lncRNAs were screened, and ESCC cases in the TCGA cohort were stratified into three clusters, which showed significantly distinct in various clinical features and immune landscapes. A RiskScore model comprising ten m6A/m5C-lncRNAs prognostic signature were constructed and displayed good independent prediction ability in validation datasets. Patients in the low-RiskScore group had a better prognosis, a higher abundance of immune cells (CD4 + T cell, CD4 + naive T cell, class-switched memory B cell, and Treg), and enhanced expression of most immune checkpoint genes. Importantly, patients with low-RiskScore were more cline benefit from immune checkpoint inhibitor treatment (P < 0.05).
Conclusion
Our findings underscore the potential of RiskScore system comprising ten m6A/m5C-related lncRNAs as effective biomarkers for predicting survival outcomes, characterizing the immune landscape, and assessing response to immunotherapy in ESCC.
Familial Dilated Cardiomyopathy in a Child: A Case Report
Authors: Ismail Ali , Khreis Dima , Assaad Amani , Majdalani Marianne Nimah 
Published: November 2023

Abstract
Background
Dilated cardiomyopathy (DCM) commonly leads to heart failure (HF) and represents the most common indication for cardiac transplantation in the pediatric population. Clinical manifestations of DCM are mainly the symptoms of heart failure; it is diagnosed by EKG, chest x- ray and echocardiography. For the idiopathic and familial diseases cases of DCM, there are no definite guidelines for treatment in children as they are treated for prognostic improvement.
Case presentation
We report the case of a 2-year-old girl diagnosed with dilated cardiomyopathy associated with homozygous mutation in the Myosin Light Chain 3 gene admitted for edema in lower extremities, muscle weakness, lethargy and vomiting, and she was found to be in cardiogenic shock. Chest x-ray showed cardiomegaly and EKG showed first degree atrioventricular block. Echocardiogram showed severe biventricular systolic and diastolic dysfunction. After 70 days of hospitalization, the patient went into cardiac arrest with cessation of electrical and mechanical activity of the heart, despite cardiopulmonary resuscitative efforts.
Conclusion
Although rare, pediatric DCM carries a high risk of morbidity and mortality and a lack of curative therapy.
Evaluation of the Relationship between ADHD and Comorbid Psychiatric Conditions: A Comprehensive Study on a Large Cohort
Authors: Sarwar Adeel , Vitetta Frank 
Published: November 2023

Abstract
This study investigates the prevalence and nature of psychiatric comorbidities in individuals diagnosed with attention-deficit/hyperactivity disorder (ADHD), with a focus on diverse gender categories including transgender and non-binary individuals. Utilizing data from 1,528 participants sourced from the ADHDtest.ai website and under the guidance of MD Adeel Sarwar, employed standardised diagnostic tools to identify ADHD and subsequent comorbid psychiatric conditions. This investigation highlights a pronounced prevalence of anxiety, particularly among non-binary (63.2%) and transgender individuals (37.5%), suggesting the need for specialized care. Additionally, we observed a substantial number of females diagnosed with ADHD (44.97%) didn’t report any pre-existing conditions, a distinctive manifestation of ADHD within this demographic group. The discussion emphasizes the intertwined nature of ADHD with other conditions and the importance of gender-specific considerations in diagnosis and treatment. The study conclusion aligns with the National Institute of Mental Health's findings on ADHD comorbidity, underscoring the imperative for a multifaceted evaluative approach to improve clinical outcomes. This research enriches the comprehension of the intricate psychiatric terrain associated with ADHD, highlighting the imperative for ongoing investigations into its concurrent disorders and the repercussions these have on tailored therapeutic approaches.
Detection of Alport gene variants in children and young people with persistent haematuria
Authors: Ng Natasha Su Lynn , Yamamura Tomohiko , Shenoy Mohan , Stuart Helen M , Lennon Rachel 
Published: November 2023

Abstract
Background
Genetic kidney disease is an important cause of persistent microscopic haematuria in children and young people. We aimed to determine the frequency of variants in the Alport syndrome genes (COL4A3, COL4A4 or COL4A5) in individuals under 18 years of age presenting with persistent microscopic haematuria to a single specialist centre in the United Kingdom over a 10-year period.
Methods
We conducted a retrospective longitudinal study of individuals referred to a tertiary paediatric nephrology service with persistent microscopic haematuria between April 2012 to 2022.
Results
224 individuals (female 51.8%) were evaluated with persistent microscopic haematuria of greater than 6 months duration. The age at presentation was 7.5±4.3 years (mean±SD). Targeted exome sequencing was performed in 134 with 91 individuals (68%) identified to have a pathogenic or likely pathogenic variant in COL4A3, COL4A4 or COL4A5. Only 49.5% of individuals with identified variants had a family history of microscopic haematuria documented and 57.1% had additional proteinuria. COL4A5 was the commonest gene affected and missense variants affecting glycine residues were the most common variant type.
Conclusion
Over two thirds of children and young people who underwent genetic testing had an identifiable genetic basis for their microscopic haematuria and over half did not have a documented family history. Genetic testing should be part of the evaluation of persistent microscopic haematuria despite a negative family history.
Enhanced quantum coherence of plasmonic resonances with a chiral exceptional point
Authors: Yuwei Lu , Liu Jing-Feng , Liu Renming , Jiang Haoxiang 
Published: November 2023

Abstract

Strong dissipation of plasmonic resonances is detrimental to quantum manipulation. In this work, we showcase the quantum coherence of plasmonic resonances can be significantly enhanced by integrating with photonic cavity operated at a chiral exceptional point (CEP), where the phase of light offers a new degree of freedom for flexibly manipulating quantum dynamics. The few-mode quantization theory is employed to demonstrate the advantages and related quantum-optics applications of the proposed hybrid cavity in both off- and on-resonance plasmon-photon coupling. For the former case, local density of states (LDOS) can evolve into sub-Lorentzian lineshape, with order-of-magnitude enhancement and linewidth narrowing compared to that without CEP. This results in the enhanced coherent light-matter interaction accompanied by reduced dissipation of polaritonic states. On resonance two mechanisms are responsible for significant improvement of quantum yield at CEP, by reducing plasmonic absorption through Fano interference and enhancing cavity radiation through superscattering. The latter allows achieving near-unity quantum yield in cryostat and enhancing room-temperature quantum yield by two orders of magnitude. Therefore, CEP-engineered plasmonic resonances can serve as a promising platform for exploring the quantum states control by virtue of the non-Hermiticity of open resonators and building high-performance quantum devices for sensing, spectroscopy, and quantum computing.
Implementation of a Crisis Resolution Team service improvement programme: A qualitative study of the critical ingredients for success
Authors: Lamb Danielle , Milton Alyssa , Forsyth Rebecca , Lloyd-Evans Brynmor , Akther Syeda , Fullarton Kate , O'Hanlon Puffin , Johnson Sonia , Morant Nicola 
Published: November 2023

Abstract

Background
Crisis Resolution Teams (CRTs) offer home-based care for people in mental health crisis, as an alternative to hospital admission. The success of CRTs in England has been variable. In response to this, the CRT Optimization and RElapse prevention (CORE) study developed and trialled a 12-month Service Improvement Programme (SIP) based on a fidelity model. This paper describes a qualitative evaluation of the perspectives of CRT staff, managers, and programme facilitators. We identify barriers and facilitators to implementation, and mechanisms by which service improvements took place.
Methods
Managers and staff from six purposively sampled CRTs were interviewed, as well as six facilitators who were employed to support the implementation of service improvement plans. Semi-structured focus groups and individual interviews were conducted and analysed using thematic analysis.
Results
A majority of participants viewed all components of the SIP as helpful in improving practice, although online resources were under-used. Perceived barriers to implementation centred principally around lack of staff time and ownership. Support from both senior staff and facilitators was essential in enabling teams to undertake the work associated with the SIP. All stakeholder groups reported that using the fidelity model to benchmark their CRT work to best practice and feel part of a ‘bigger whole’ was valuable.
Conclusion
CRT staff, managers and programme facilitators thought that a structured service improvement programme helped to increase fidelity to a best practice model. Flexibility (from all stakeholders) was key to enable service improvement actions to be manageable within time- and resource-poor teams.
The landscape of Hepatocellular Carcinoma and Tumor-associated Telocytes Integrated Single-nucleus RNA Sequence and Transcriptomics Technology
Authors: Xu Ying , Li Mengyu , Zhang Jinqing , Ding Ning 
Published: November 2023

Abstract
Background: Hepatocellular carcinoma (HCC) is the main malignant tumor in humans, known for its high metastasis and lethality. However, the molecular mechanisms underlying tumorigenesis in HCC are still unclear.
Methods: To address this, we conducted a comprehensive analysis using single nucleus RNA sequencing (snRNA-seq) assay and transcriptomic technology. This approach allowed us to uncover genetic changes in cancer cells and other cells within the tumor microenvironment, leading to the identification of potential hub genes for HCC.
Results: Our study analyzed snRNA-seq and transcriptomics data, revealing novel differentially expressed genes (DEGs) in HCC and identifying characteristic biomarkers for telocytes (TCs) through bioinformatics analysis. Notably, STAB2, OIT3, BMPER, MARCO, CRHBP, and FCN2 showed high specificity and sensitivity in predicting the prognosis or development of HCC.
Conclusion: TMC5 and TRABD2B were identified as unique biomarkers for TCs in the liver. These DEGs warrant further investigation to validate their potential implications in HCC.
Assessment of American Bullfrog (Lithobates catesbeianus) spreading in the Republic of Korea using rule learning of elementary cellular automata
Authors: Oh Gyujin , Wi Yunju , Kang Hee-Jin , Cheon Seung-ju , Sung Ha-Cheol , Kim Yena , Jin Hong Sung 
Published: November 2023

Abstract

The spread of American Bullfrog, one of the 100 of the World’s Worst Invasive Alien Species, has a great impact on the surrounding ecosystem. Little is known about the tendancy and pattern of how they are spreading in South Korea geographically. It is important to study the tendancy of their spreading so that a proper mitigation can be applied when needed. This study is based on the results of national surveys that observed the distribution. The entire data is divided into 25 regional clusters using the divisive hierarchical clustering method. In order to estimate the degree of spreading, a sequence of spatial distribution is constructed for each cluster using the agglomerative clustering method. ECA(elementary cellular automata) is introduced to find rules governing the pattern variation in the sequence. Each cell represents either the observed or unobserved site of bullfrog. The number of Bullfrog Observed Site (BOS) in a sequence of each cluster is counted and used to define the spreading intensity. The rules of ECA are trained and learned by the CNN(Convolution Neural Network) method and used to estimate and predict the spreading intensity by counting the number of BOS. Taking environmental factors into account, habitat suitability is used and obtained using Maxent. The spreading intensity is multiplied by the habitat suitability to get an assessment of bullfrogs spreading. The relative spreading assessment is estimated, which is classified into 4 groups; spreading intensively, spreading slowly, maintaining or declining population.
Metabolomic and Transcriptomic Analyses Reveals Candidate Genes and Pathways Involved in Secondary Metabolism in Bergenia purpurascens
Authors: Yu Fang , Zhang Xuebin , Xu Nuomei , Chen Jingyu , Lyu Xin , Wu Yufeng , Zeng Hongyan , Zhu Qiankun 
Published: November 2023

Abstract
Bergenia purpurascens is an important medicinal, edible and ornamental plant. The lack of omics information hinders the study of its metabolic pathways and related genes. In order to investigate candidate genes and pathways involved in secondary metabolism in B. purpurascens, roots, stems and leaves of B. purpurascens were subjected to metabolomic and transcriptomic analyses in this study. A total of 351 differentially accumulated secondary metabolites were identified. We identified 111 candidate enzyme genes in phenylpropanoid and flavonoid biosynthesis pathway. 22 candidate key genes related to phenylpropanoid and flavonoid synthesis were obtained by WGCNA analysis, from which 14 structural genes in phenylpropanoid and flavonoid synthesis and five transcription factors related to flavonoid synthesis were identified. This study provides data resources and new insights for further studies on the biosynthesis of major active components in B. purpurascens.
Sources of resistance to Fusarium oxysporum f. sp. vanillae in Vanilla spp
Authors: Bautista-Aguilar José R , Iglesias-Andreu Lourdes G. , Luna‑Rodríguez Mauricio , Sánchez-Coello Nadia G 
Published: November 2023

Abstract
Fusarium oxysporum f. sp. vanillae (Fov) is the most devastating pathogen of the vanilla crop. A possible solution to this phytosanitary problem is to obtain promising genotypes with some degree of resistance to Fov. The use of biotechnological tools has been very useful for this purpose, so the present study was proposed to evaluate the degree of resistance to Fov in four accessions of Vanilla: Vanilla F1 hybrid (V. planifolia x V. pompona), V. planifolia "RH350" (V. planifolia resistant to Fov strain H3), V. planifolia "La Gran Lucha" (LGL, wild morphotype from Oaxaca, Mexico), and V. planifolia "Mansa" (commercial morphotype from Veracruz, Mexico). For this purpose, the mean lethal concentration (LC50) of the culture filtrates (FC) obtained from the pathogenic strain M21C5 of Fov was first determined based on the survival of V. planifolia shoots. The LC50 value for the Fov (strain evaluated) was determined to be 35%. Differences were found in the survival of in vitro seedlings subjected to 40% of the Fov culture filtrates. The highest survival values (75% and 70%) were found in V. planifolia "RH350" and V. planifolia "La Gran Lucha" accessions, respectively. In vivo tests under greenhouse conditions indicated that V. planifolia "RH350" and V. planifolia "La Gran Lucha" showed high values of survival (44± 2.6 and 37± 2.2, respectively) and Fov resistance (36±2.1 and 30±1.8, respectively). The results obtained in this study are of great interest for the development of genetic improvement work currently being developed for this crop.