bluuebunny/crossref_metadata_2025_split huggingface dataset:
Panorama™ Ab Microarray Cell Signaling kit: A unique tool for protein expression analysis
Authors: Kopf Eliezer , Shnitzer Dalia , Zharhary Dorit 
Published: May 2005

AbstractAntibody arrays are a promising new tool for mass analysis of protein level changes in cells responding to different stimuli. Here we describe a novel antibody array system called Panorama™ Ab Microarray Cell Signaling, that contains 224 antibodies spotted on FAST™ nitrocellulose‐coated slides that can detect protein levels as low as a few nanograms per mL. The antibodies spotted are specific for proteins important in various areas of cell signaling such as phosphorylation, cell cycle, apoptosis, nuclear signaling and cytoskeleton proteins. Furthermore, for some of the protein targes, the Panorama™ Ab Microarray can detect phosphorylated and nonphosphorylated forms of the traget protein. We found that treatment of the slides post‐spotting is important for the array performance (ratio of signal to background) and its stability. Panorama™ Ab Microarray was used to analyze changes in protein expression in F9 embryonic carcinoma cells stimulated to differentiate by all‐trans retinoic acid. We found that the level of several proteins, among them cell cycle regulators and kineases, was either up‐ or down‐regulated. For more than ten protein targets, the results obtained by the Panorama™ Ab Microarray were confirmed by immunoblotting.
Structural defects in poly(vinyl chloride)
Authors: Starnes William H. 
Published: May 2005

AbstractThis article describes, in narrative style, the research of the author and his associates, performed over a period of 30 years, that led to the identification and quantification of the anomalous structures in poly(vinyl chloride) (PVC) and to detailed descriptions of their mechanisms of formation. Also examined here are the implications of this work for the thermal stability of PVC, for the overall chemical mechanism of vinyl chloride polymerization, and for other relevant aspects of the chemistry and technology of vinyl chloride homopolymers and copolymers. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 2451–2467, 2005
Coping among older‐adult, long‐term cancer survivors
Authors: Deimling Gary T. , Wagner Louis J. , Bowman Karen F. , Sterns Samantha , Kercher Kyle , Kahana Boaz 
Published: May 2005

AbstractThis research focuses on the coping behavior of older adult, long‐term cancer survivors. Specifically, it identifies the personal (including race and gender) and illness/treatment characteristics of survivors that are significantly associated with the use of specific coping styles: planning, acceptance, venting, denial and seeking social support. It also examines the mediating role that these forms of coping play in terms of psychological distress: anxiety, depression and cancer‐related worries. Multivariate analysis of data from a random sample of 321 long‐term survivors in a major cancer center tumor registry is used to address the above issues. The most prominent forms of coping used by long‐term survivors were planning and acceptance; least used were venting and denial. Increased age was associated with lower use of all forms of coping, but cancer type was not. Denial as a form of coping was associated with higher anxiety, depression and cancer‐related worries. While race was not found to be a significant predictor of coping style, it was a significant predictor of three dimensions of psychological distress, anxiety, depression and cancer‐related worries, with African Americans exhibiting lower levels of distress than Whites. The relevance of these findings for health and social service practitioners is discussed. Copyright © 2005 John Wiley & Sons, Ltd.
Risk factors of bronchial hyperresponsiveness in children with wheezing‐associated respiratory infection
Authors: Futrakul Sitthivuddhi , Deerojanawong Jitladda , Prapphal Nuanchan 
Published: May 2005

AbstractThe objectives of this study were to identify possible risk factors of bronchial hyperesponsiveness (BHR) in children up to 5 years of age with wheezing‐associated respiratory infection (WARI), and to study the prevalence of BHR. Children up to 5 years of age with WARI were enrolled in the study. The parents or caregivers of children were asked about their demographic data and clinical histories. Physical examination and clinical score assessment were performed. Pulmonary function tests, i.e., tidal breathing flow volume (TBFV), were performed to measure tidal breathing parameters before and after salbutamol nebulization. If volume at peak tidal expiratory flow/expiratory tidal volume and time to peak expiratory flow/total expiratory time increased ≥20%, or tidal expiratory flow at 25% of tidal volume/peak tidal expiratory flow increased ≥20% after nebulization therapy, BHR was diagnosed. The number in the positive BHR group was used to calculate the prevalence of BHR, and clinical features were compared with those of the negative BHR group. Categorical data were analyzed for statistical significance (P < 0.05) by chi‐square test or Fisher's exact test, or Student's t‐test, as appropriate. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated for those with statistical significance. One hundred and six wheezing children underwent pulmonary function tests before and after salbutamol nebulization. With the aforementioned criteria, 41 cases (38.7%) were diagnosed with BHR. History of reactive airway disease, (OR, 6.31; 95% CI, 1.68–25), maternal history of asthma (OR, 3.45; 95% CI, 1.34–9), breastfeeding less than 3 months (OR, 3.18; 95% CI, 1.26–8.12), and passive smoking (OR, 3; 95% CI, 1.15–7.62) were significant risk factors of BHR. The eosinophil count was significantly higher in the BHR (+) group particularly, in children 1–5 years of age (P ≤ 0.01). Patchy infiltrates were more commonly found in patients with negative BHR but not statistically significant. In conclusion, a history of reactive airway disease, maternal history, breastfeeding less than 3 months, and passive smoking were significant risk factors for BHR. Pediatr Pulmonol. © 2005 Wiley‐Liss, Inc.
Sildenafil and an early stage of chronic hypoxia‐induced pulmonary hypertension in newborn piglets
Authors: Binns‐Loveman Karen M. , Kaplowitz Mark R. , Fike Candice D. 
Published: May 2005

AbstractDevising therapies that might prevent the onset or progression of pulmonary hypertension in newborns has received little attention. Our major objective was to determine whether sildenafil, a selective phosphodiesterase inhibitor, prevents the development of an early stage of chronic hypoxia‐induced pulmonary hypertension in newborn pigs. Another objective was to determine whether sildenafil causes pulmonary vasodilation without systemic vasodilation in piglets with chronic pulmonary hypertension. Piglets were raised in room air (control, n = 5) or 10–11% O2 (hypoxic, n = 17) for 3 days. Some piglets (n = 4) received oral sildenafil, 12 mg/kg/day, throughout exposure to hypoxia. All piglets were anesthetized and catheterized, and pulmonary arterial pressure (Ppa), pulmonary wedge pressure (Pw), aortic pressure (Ao), and cardiac output (CO) were measured. Then for some piglets raised in hypoxia for 3 days, a single oral sildenafil dose (3 mg/kg, n = 6) or placebo (n = 5) was given, and hemodynamic measurements were repeated. For piglets raised in hypoxia for 3 days, mean Ppa and calculated PVR were elevated above respective values in control piglets. Mean Ppa and PVR did not differ between piglets that received sildenafil throughout exposure to hypoxia and those that did not. For piglets with chronic hypoxia‐induced pulmonary hypertension that received a single oral dose of sildenafil, mean Ppa and PVR decreased, while mean Pw, CO, mean Ao, and systemic vascular resistance remained the same. All hemodynamic measurements were unchanged after placebo. Oral sildenafil did not influence the early stage of chronic hypoxia‐induced pulmonary hypertension in newborn piglets. However, a single oral dose of sildenafil caused pulmonary vasodilation, without systemic vasodilation, in piglets with chronic hypoxia‐induced pulmonary hypertension, which may have therapeutic implications. © 2005 Wiley‐Liss, Inc.
Poly[2‐methoxy‐5‐(2′‐ethyl‐hexyloxy)‐1,4‐phenylene vinylene] (MEH‐PPV) thin film spin‐coated on porous n‐type GaAs
Authors: Beji L. , Ben Jomaa T. , Ltaeif A. , Bouazizi A. 
Published: May 2005

AbstractPoly[2‐methoxy‐5‐(2′‐ethyl‐hexyloxy)‐1,4‐phenylene vinylene] (MEH‐PPV) was grown on porous GaAs (π‐GaAs) by spin coating and studied via scanning electron microscopy (SEM) and room temperature photoluminescence (PL). The SEM investigation revealed that MEH‐PPV was grown deeply in the porous structure and had a filament‐like shape. The PL result showed that a strong quenching effect occurred when using π‐GaAs. This effect was suggested to be due to photoinduced charge transfer at the MEH‐PPV/GaAs nanocrystallite interface. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Investigation of the ac magnetic field dependence of the first and the third harmonics of the ac susceptibility in a granular Bi‐2223 sample
Authors: Ozogul O. 
Published: May 2005

AbstractThe field and temperature dependencies of the first and third harmonics of the ac magnetic susceptibilities of a granular Bi‐2223 superconductor have been investigated. Model calculations have been performed on the basis of a theoretical model that incorporates collective creep concepts in a critical‐state equation by considering the temperature‐dependent effective volume fraction. The predictions of the model for the field and temperature dependencies of the first and third harmonics were found to be in good agreement with experimental data. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Humidity and temperature dependences of oxide lines fabricated by atomic force microscopy based nanolithography
Authors: Ai F. , Wang J. C. , Luo L. , Liu Y. , Zhou Y. F. , Pan Z. L. 
Published: May 2005

AbstractLocal nano‐oxidation has been used to write oxide lines on hydrogen‐passivated silicon surfaces by atomic force microscopy. The experimental results suggest that the temperature and humidity have considerable influence on the dimension of as‐grown oxide lines. It is concluded that the size of oxide lines (width and height) increase following the increase of relative humidity or the decrease of temperature of substrate, these can be explained by the variation of the amount of absorbed water layer on the sample surface under different parameters. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Complementary investigations using the MCDF and RQDO methods
Authors: Charro E. , Martín I. 
Published: May 2005

AbstractTheoretical oscillator strengths for fine‐structure lines of electric dipole (E1) allowed transitions for a number of ions belonging to the Be, As, and Ge sequences, are reported. Both ab initio (multiconfiguration Dirac–Fock) and model potential (relativistic quantum defect orbital) techniques have been used. A comparative analysis of the two sets of f‐values, as well as with other data found in the literature, has been carried out. For most of the studied ions and transitions, the results achieved with the two methodologies are, overall, in rather good agreement. © 2005 Wiley Periodicals, Inc. Int J Quantum Chem, 2005
Surgical lessons from the Intergroup Rhabdomyosarcoma Study (IRS) pertaining to extremity tumors
Authors: Lawrence Walter , Hays Daniel M. , Heyn Ruth , Beltangady Mohan , Maurer Harold M. ,   Intergroup Rhabdomyosarcoma Study Committee
Published: May 2005

AbstractThe large number of patients entered into the Intergroup Rhabdomyosarcoma Study (IRS) has allowed a study of prognostic factors and clinical management decisions for a specific primary site‐the extremities. A retrospective review of IRS I (1972–1978) and IRS II (1978–1984) included 217 patients with sarcomas of the extremities of 1,415 patients without distant metastases. Disease‐free survival and survival data from this subgroup of patients demonstrated that: (a) the UICC clinical staging system has clear‐cut validity, (b) total gross resection of sarcomas of the extremities is an optimal therapeutic approach in spite of proven benefits from nonoperative therapies, (c) results of treatment are improved by reexcision of the initial resection site if the histologic margin is involvedor the initial resection was an unplanned procedure for diagnosis, (d) regional lymph node biopsy or dissection is an important procedure for planning and conduct of the treatment program, and (e) vigorous and thorough surgical procedures are required for optimal end results of treatment of extremity sarcomas despite the proven value of nonoperative therapies.
Immediate definitive surgery for perforated duodenal ulcer does not increase operative mortality: A prospective controlled trial
Authors: Hay Jean ‐Marie , Lacaine François , Kohlmann Gérard , Fingerhut Abe ,   Association for Surgical Research
Published: May 2005

AbstractOne hundred and sixty‐two patients with nonsealed perforated duodenal ulcer have been studied prospectively. These patients were randomized to undergo simple closure (83 patients) or closure and vagotomy (79 patients); the vagotomy was either a truncal vagotomy (71 patients) or a proximal gastric vagotomy (8 patients). One operative death was recorded in the simple closure group, that of a 92‐year‐old woman operated 36 hours after the onset of perforation, who died on the seventh postoperative day of infectious pulmonary complications. There were no deaths in the vagotomy group. The mortality in the 2 groups, simple closure and vagotomy, can be assumed to be similar according to a decision‐making rule based on confidence interval limits (p<0.05). Complications and postoperative course were similar in both groups except for wound infection, which was more frequent in the vagotomy group than in the simple closure group (p<0.05). Two previous prospective clinical trials have shown that relief of symptoms was better in the group treated by definitive surgery than in the control group treated by simple closure. Demonstration of similar mortality rates in simple closure and vagotomy groups failed in these 2 trials because of a high type II error. We conclude therefore, that because of equal postoperative mortality (p<0.05) and better long‐term results, definitive surgery is better than simple closure in the treatment of nonsealed perforated duodenal ulcer.
Lymphography of the breast as an accessory in the early diagnosis and detection of carcinoma of the breast: Lymphography as a prognostic tool
Authors: Rosenblum Izhak , Wischnitzer Theodor , Stadier Jona A. , Bar‐On M. Jack 
Published: May 2005

AbstractRadionuclide lymphography of the internal mammary and axillary lymph nodes performed on 63 patients with suspected carcinoma of the breast showed good correlation with the histological findings. Twenty‐seven of these patients proved to have carcinoma, 14 with lymph node involvement and 13 without. The remaining 36 had benign lesions.Radionuclide lymphography was accurate in 60 of the 63 cases (95%), giving a sensitivity of 13/14(93%) and a specificity of 47/49(96%). Thus, the technique appears to be sufficiently accurate to suggest its use as a routine noninvasive preoperative examination, enabling the surgeon to reach a decision on patient management, type of operation or radiation therapy. Important additional factors influencing this decision are the possibility of crossover in lymphatic drainage from one breast to the other or a possible malignant lesion on the breast that was not biopsied and would thus go undetected had lymphography not been performed.
Relationship Between Successful Ablation Sites and the Scar Border Zone Defined by Substrate Mapping for Ventricular Tachycardia Post‐Myocardial Infarction
Authors: VERMA ATUL , MARROUCHE NASSIR F , SCHWEIKERT ROBERT A. , SALIBA WALID , WAZNI OUSSAMA , CUMMINGS JENNIFER , ABDUL‐KARIM AHMAD , BHARGAVA MANDEEP , BURKHARDT J. DAVID , KILICASLAN FETHI , MARTIN DAVID O. , NATALE ANDREA 
Published: May 2005

Introduction: It is unknown if identification of scar border zones by electroanatomical mapping correlates with successful ablation sites determined from mapping during ventricular tachycardia (VT) post‐myocardial infarction (MI). We sought to assess the relationship between successful ablation sites of hemodynamically stable post‐MI VTs determined by mapping during VT with the scar border zone defined in sinus rhythm. Methods and Results: Forty‐six patients presenting with hemodynamically stable, mappable monomorphic VT post‐MI and who had at least one such VT successfully ablated were prospectively included in the study. In each patient, VT was ablated by targeting regions during VT that exhibited early activation, ± isolated mid‐diastolic potentials, and concealed entrainment suggesting a critical isthmus site. Prior to ablation, a detailed sinus‐rhythm CARTO voltage map of the left ventricle was obtained. A voltage <0.5 mV defined dense scar. Successful VT ablation sites were registered on the sinus voltage map to assess their relationship to the scar border zone. Of the 86 VTs, 68% were successfully ablated at sites in the endocardial border zone. The remaining VTs had ablation sites within the scar in (18%), in normal myocardium (4%), and on the epicardial surface (10%). There were no significant differences in VT recurrence amongst the different groups. Conclusion: Successful ablation sites of hemodynamically stable, monomorphic VTs post‐MI are often located in the scar border zone as defined by substrate voltage mapping. However, in a sizable minority, ablation sites are located within endocardial scar, epicardially, and even in normal myocardium.
Method to Predict Isthmus Location in Ventricular Tachycardia Caused by Reentry with a Double‐Loop Pattern
Authors: CIACCIO EDWARD J. , TOSTI ALEXIS C. , SCHEINMAN MELVIN M. 
Published: May 2005

Background: During electrophysiologic study, induction and mapping of clinical reentrant ventricular tachycardia can be difficult. Hence, analysis of sinus‐rhythm electrograms for reentry localization is of potential clinical relevance. Herein is described a method of sinus‐rhythm electrogram shape analysis, that does not require arbitrary threshold values, for localization of double‐loop reentrant circuits that drive clinical tachycardias. Methods and Results: Reentrant ventricular tachycardia was induced by premature stimulation in 23 postinfarction canine hearts 4–5 days after left anterior descending (LAD) ligation. Sinus‐rhythm activation maps were constructed from bipolar electrograms acquired at 196–312 sites in the epicardial border zone. The timing of all electrogram peak deflections during one cycle of sinus rhythm was determined, and the mean (MPD) and deviation (DPD) were taken, respectively, as estimates of the time of activation wavefront crossing, and the duration of local electrical activity. These variables were then mapped on a computerized grid. The line of most uniform and sharp MPD gradient predicted the propagation direction through the double‐loop reentrant circuit isthmus that would occur upon tachycardia induction. The sharpest transitions in DPD bounding this line predicted the positions of arcs of block that would border the reentrant circuit isthmus during tachycardia. The actual and estimated isthmuses overlapped by a mean of 84.1 ± 3.8%. In six experiments lacking inducible reentrant tachycardia, no line of sharp MPD gradient was present in the MPD maps. Conclusions: Analysis of multiple sinus‐rhythm deflections can localize the reentrant ventricular tachycardia isthmus without introduction of arbitrary threshold points and peak choices that may lead to error.
Decomposition of high-pressure (400 kPa) SF6–CO2, SF6–CO, SF6–N2–CO2and SF6–N2–CO mixtures under negative dc coronas
Authors: Casanovas A M , Casanovas J 
Published: May 2005

Sulfur hexafluoride, SF6, is successfully employed by the electric power industry for gas insulated equipment. However, it is a potent greenhouse gas and mixtures of SF6with more friendly environmental gases have recently been proposed. It was demonstrated that SF6mixed with N2and/or CO2could be a good substitute for some insulation applications such as gas-insulated transmission lines (GILs).Considering the possible occurrence of corona discharges in GILs, the decomposition of these gas mixtures under such stress must be studied. In this paper, the chemical stability under negative corona of SF6and SF6–N2(10 : 90) or (5 : 95) mixtures was investigated at 400 kPa on adding percentages of CO2up to 80%.The corona discharges were generated at 23°C in a 340 cm3experimental cell between a stainless steel point connected to a high voltage source and a plane of aluminium. The gaseous decomposition products were assayed by gas chromatography at the end of each run carried out over a range of transported charges covering 8–17 C.The presence of CO2in SF6and SF6–N2mixtures submitted to negative coronas leads to:a considerable increase in the formation of the major compound group (SOF4+SO2F2),formation of similar large quantities of COF2,abundant production of CO with diluted SF6in nitrogen,decreased formation of S2F10.
Complementary and Alternative Medicine in Pakistan: Prospects and Limitations
Authors: Shaikh Babar T. , Hatcher Juanita 
Published: May 2005

Despite all the marvelous advancements in modern medicine, traditional medicine has always been practiced. More than 70% of the developing world′s population still depends on the complementary and alternative systems of medicine (CAM). Cultural beliefs and practices often lead to self‐care or home remedies in rural areas and consultation with traditional healers. Evidence‐based CAM therapies have shown remarkable success in healing acute as well as chronic diseases. Alternative therapies have been utilized by people in Pakistan who have faith in spiritual healers, clergymen, hakeems, homeopaths or even many quacks. These are the first choice for problems such as infertility, epilepsy, psychosomatic troubles, depression and many other ailments. The traditional medicine sector has become an important source of health care, especially in rural and tribal areas of the country. The main reasons for consulting a CAM healer is the proximity, affordable fee, availability, family pressure and the strong opinion of the community. Pakistan has a very rich tradition in the use of medicinal plants for the treatment of various ailments. It necessitates the integration of the modern and CAM systems in terms of evidence‐based information sharing. The health‐seeking behavior of the people especially in developing countries calls for bringing all CAM healers into the mainstream by providing them with proper training, facilities and back‐up for referral. A positive interaction between the two systems has to be harnessed to work for the common goal of improving health of the people.Corrigendum to
“Complementary and Alternative Medicine in Pakistan: Prospects and Limitations”dx.doi.org/10.1093/ecam/nel113