bluuebunny/crossref_metadata_2025_split huggingface dataset:
DEVELOPMENT OF THE RECYCLING PROCEDURE FOR RAPID ANTIGEN TESTS
Authors: Rudolf Rebeka , Feizpour Darja , Jelen Žiga , Majerič Peter , Švarc Tilen , Zadravec Matej , Gomboc Timi , Kocijan Aleksandra 
Published: February 2024

The article presents the problem of rapid antigen tests when they become mass waste after use. Based on this, the hypothesis was made that rapid antigen tests can be recycled. Rapid antigen tests, which were used in the Covid-19 epidemic to quickly detect infections in the population or to confirm the presence of the Sars-Cov 2 virus in patients, were intended to limit the spread of the epidemic. To confirm the hypothesis of recycling for rapid antigen tests, the LFIA-REC ATP 150 project was prepared, which was selected for co-financing by the Norwegian Fund.
Rapid antigen tests consist of a sample and conjugate pad, detectable or nitrocellulose membranes and absorbent pads and a plastic case. The function of the sample pad is to evenly absorb the sample (mucus, blood) and lead it to the conjugate pad with a steady flow. Gold nanoparticles (labels) are deposited on the conjugate pad. The key is that the gold nanoparticles are conjugated with capture molecules capable of binding to potentially present antibodies or virus in the sample.
The scope of the research problem thus required the inclusion of various characterization techniques that must be applied to the individual material in the rapid antigen test to subsequently develop an efficient recycling process for the rapid antigen tests. The result of the research presented in this paper represents a newly developed algorithm of characterization techniques, which includes a recommended description of the preparation of samples of key materials from rapid antigen tests. This algorithm successfully achieved the characterization of gold nanoparticles from rapid antigen tests. Based on the developed algorithm, the final part of the project will validate the recycling process of rapid antigen tests, so that they can be recycled, i.e. gold nanoparticles or plastic used in new products. The paper presents the algorithm of characterization techniques with a description of the preparation of material samples from rapid antigen tests.
Preclinical Assessment of <i>Crocus sativus</i> Extract Loaded Nano Emulsion for Scopolamine Induced Cognitive Impairment: A Comprehensive Safety and Efficacy Study
Authors: Parikh Namrata , Patel Komal 
Published: February 2024

Background
Crocus sativus is popular herb used to improve and treat the memory, cognition related functions of brain and thereby impose significant role in improve and maintain brain health. The medicinal important part of Crocus sativus is stigma of flower which is rich in apocarotenoids crocin, picrocrocin, safranal and many more volatile compounds. The pharmacological actions such as antioxidant, anti-amyloidogenic, anti-depressant, anti depressant, anti cancer, anti inflammatory etc. properties are attributed due to of presence of caratenoids. However the poor stability, solubility and enzymatic degradation of bioactives of Crocus sativus have substantially limited their therapeutic application. In this study, a saffron nano emulsion of carotenoid rich fraction of Crocus sativus extract was assessed for its safety and efficacy.
Methodology
The single dose acute oral toxicity assessment of nanoemulsion was performed as per OECD 420 to determine the lethal dose (LD50). The subacute repeated 28 days oral toxicity was performed to provide the effective dose for the preclinical assessment as per OECD guideline 407 The animals were observed during the entire toxicity experimentation for any toxic signs and changes of hematological, biochemistry parameters. The efficacy study of saffron nano emulsion was evaluated by two behavioral test models- Passive avoidance test and Morris water maze test using scopolamine induced memory impairment.
Results & Conclusions
Animals administered 500 mg/kg/day as single dose orally did not cause any signs of toxicity or mortality in rats and results of acute toxicity assessment of saffron nano emulsion has indicated that the NOAEL (No Observed Adverse Effective Level) of saffron nano emulsion was found to be 500 mg/kg. In subacute toxicity study three different dose of 75, 100 & 125 mg/kg/day for 28 days administered orally in animals revealed no significant changes in body weight, hematological, biochemical parameters. The histopathological study has indicated no pathological changes observed in the vital organs of rats treated with the 125 mg/kg/day.
The efficacy assessment of saffron nano emulsion using two behavioral models have suggested that nano emulsion at the dose of 10 mg/kg/day was significantly reduced the Scopolamine induced memory impairment.
Addiction Patient’s Relationship to Self and Predictions on the Estimated Hospitalization Duration
Authors: Lescai Alina-Maria , Anghele Mihaela , Baltă Alexia Anastasia , Dumitrache Anghele Aurelian , Dragomir Liliana , Ciubara Bogdan 
Published: February 2024

Addictions are physical and/or psychological dependency disorders characterized by repetitive and compulsive behaviours in which the individual has difficulty controlling impulses. In this study, behaviours are defined as chronic alcohol or substance use. Thus, compulsions will generate negative consequences on a person's quality of life. Among the causes of addiction are genetic and biological factors, environmental factors, trauma, abuse, lack of emotional support, stress, social pressures, poor coping mechanisms, social and cultural factors.
Material and method
In this study, part of a larger study, 81 patients diagnosed with toxic-nutritional liver cirrhosis, chronic alcohol users, aged between 32 and 68 years were included. The study period was of two years, and anamnestic data (number of hospitalization days, hospitalization frequency) were collected for the period 2015-2022. In order to carry out the linear research, SPSS statistical software was used. Patients, after obtaining consent, were administered a psychological questionnaire designed to assess unconditional self-acceptance, based on the hypothesis that low self-tolerance generates self-destructive behaviours, i.e. addictions.

Results
The necessary statistical steps were followed in order to check the database and it was possible to obtain correlations between the number of hospitalization days, the hospitalization frequency and the scores obtained in the questionnaire. Finally, it was possible to generate a simple linear regression prediction with the number of hospitalisation days /inpatient frequency as the dependent variable and the test score as the independent variable.
The results showed that as unconditional self-acceptance decreases (decreasing score), the hospitalization duration or the number of patient presentations to the doctor increases.
Conclusions
Unconditional self-acceptance, environmental tolerance, coping mechanisms have a major impact on the patient's well-being and compliance with treatment. Psychosomatic disorders accompany the addiction patient to a much greater extent than the diagnosis made by clinicians. This demonstrates the need for a diagnostic tool, the lack of collaboration with the psychiatrist, ultimately generating costs on the health system and reducing the quality of the patient’s life.
In order to optimise the diagnosis, a tool within the clinician's reach (internal medicine doctor, gastroenterologist, etc.) and a real collaboration with the psychiatrist or clinical psychologist is necessary.
PENGEMBANGAN KELOMPOK USAHA MADU KELULUT DI DESA ANTAR RAYA KECAMATAN MARABAHAN KABUPATEN BARITO KUALA
Authors: Irwansyah  , Saladin Ghalib  , Siti Rusidah  , Fitriyadi  , Sugiharto  , Riski Muslim Hidayat  
Published: February 2024

ABSTRAK: Madu merupakan sumber komoditi yang banyak diperlukan bagi industri farmasi, kosmetik, dan makanan, di samping konsumsi sehari-hari. Madu menurut hasil riset diketahui mengandung 24 macam zat gula, di samping mengandung zat ferment, vitamin mineral, asam, asam-asam amino, hormon, zat bakterisidal an bahan-bahan aromati. Adapun kegiatan – kegiatan yang dilakukan oleh para peserta pada kegiatan Pengabdian pada masyarakat didesa tersebut adalah Ramah Tamah & Focus Group discussion (FGD) dan Kegiatan Kunjungan ke kelompok UMKM. Pelaksanaan kegiatan Pengabdian Pada masyarakat (P2M) yang diprogram secara konsepsi dengan menargetkan sasaran masyarakat kelompok pelaku usaha kecil, dengan menyesuaikan antara basis keilmuan dengan realitas lapangan adalah merupakan hal yang dinilai sangat baik untuk selalu dipertahankan dan ditingkatkan. Begitu juga demikian halnya konsepsi dari Pengabdian Pada Masyarakat (P2M), yang selama ini telah berdasarkan atas kebutuhan masyarakat dalam hal ini Kelompok Usaha dan bukan hanya paket program yang jauh dari kebutuhan dan permasalahan yang dihadapi oleh masyarakat khususnya pada masyarakat kelompok Usaha Madu kelulut desa Antar raya kecamatan Marabahan Batola.
Kata kunci : Kelompok Usaha, Madu Kelulut, Desa Antara Raya

ABSTRACT: Kelulut honey is a commodity source that is much needed for the pharmaceutical, cosmetic and food industries, in addition to daily consumption. Honey, according to research results, is known to contain 24 kinds of sugars, in addition to containing ferment substances, vitamins, minerals, acids, amino acids, hormones, bactericidal substances and aromatic ingredients. The activities carried out by the participants in the Community Service activities in the village were Friendly & Focus Group Discussions (FGD) and Visits to MSME groups. The implementation of Community Service activities which are programmed conceptually by targeting the community as small business actors, by adjusting the scientific base with the realities of the field is something that is considered very good to always be maintained and improved. Likewise, the concept of Community Service, which so far has been based on the needs of the community, in this case the Business Group and not just a program package that is far from the needs and problems faced by the community, especially in the Kelulut Village Honey Business group. highway Marabahan Batola sub-district. Keywords: Business Group, Kelulut Honey, Antara Raya Village
Keywords: Business Group, Kelulut Honey, Antara Raya Village
Distribution, Accumulation and Translation Characteristics of Heavy Metals CD in Different Varities of Edible Rape Under CD Stress
Authors: Liu Jing , Kang Yun Ling , Du Feng Lian , Liao Qiang Shang , Dong Wei , Ma Ting Mao , Zou Yuan Guan , Li Jiang Shun 
Published: February 2024

To investigate the differences in cadium (Cd) uptake and translocation among edible rapes and select suitable rape varieties for safe production, a field experiment was carried out with 25 varieties of edible rapes on a farmland polluted with Cd in the central souothern part of Hunan Province. The growing characteristics and Cd contents in rape tissues were measured, and the amount of Cd removed was calculated. The results showed that the growth of 25 rape varieties was not inhibited without withering or inconsistent changes in leaf and so on. The Cd contents in stem-leaf and root of rapes were in the range 0.05-0.26mg·kg-1 and 0.04-0.26mg·kg-1, respectively. Basing on BCF, the stem-leaf showed a greater capacity for Cd transport than root. And the total Cd removed by rape were 0.002-0.016 mg·plant-1. Except for plant height, BCF of soil available Cd from soil to stem-leaf, TCF of Cd from root to stem-leaf and Cd taken away by rape, there was no significant difference among edible rape varieties. Although Cd contents in stem-leaf of 25 rape varieties does not exceed the national safety standards, planting “Lvjin 1”, “Guanyou Qingjing” and “Guanyou brassica” not only reduced soil pollution but also allowed the production of safe leafy rape.
Los alfares islámicos en el entorno de las Ollerías (Córdoba): dispersión, cronología y tipología
Authors: Salinas Pleguezuelo Elena 
Published: February 2024

En este trabajo se realiza una aproximación a los hornos y alfares islámicos cordobeses, especialmente a raíz de los hallazgos de los últimos años. Se han analizado aspectos como la distinta tipología de los hornos cordobeses, su evolución a través de los siglos islámicos –desde época emiral a almohade-, la ubicación de las distintas áreas alfareras en Madīnat Qurtuba, principalmente la zona de las Ollerías, y los elementos necesarios en un alfar.
This paper presents a summary of the Islamic pottery craft areas and kilns in the medieval Cordoba, especially in the light of the recent findings. For this study, different types of kilns, its evolution through the Middle Ages -from the Emiral to the Almohad periods-, the location of the different pottery craft areas of Madīnat Qurtuba -mainly the Ollerías area- and the elements needed have been analysed.
Sucralose or rebaudioside A at recommended doses did not alter the gut microbiota composition in rats under two dietary conditions
Authors: Ramos-García Meztli , Genis-Mendoza Alma Delia , García-Vázquez Carlos , Martínez-Magaña José Jaime , Olvera-Hernández Viridiana , Martínez-López Mirian Carolina , Díaz-Zagoya Juan Cuauhtémoc , Alvarez-Villagomez Carina Shianya , Juárez-Rojop Isela Esther , Nicolini Humberto , Ble-Castillo Jorge Luis 
Published: February 2024

Abstract
Research reported that non-nutritive sweeteners (NNS), including sucralose, elicit metabolic changes through the gut microbiota (GM) modulation, nonetheless, this topic remains controversial. Furthermore, the impact of rebaudioside A (reb A) on GM has received limited scrutiny. Consequently, we aim to investigate the response of GM composition to both sucralose and reb A in rats, considering two distinct dietary conditions. Male Wistar rats (150–200 g) fed either a normal diet (ND) or a high-fat diet (HFD) were randomly assigned to receive sucralose (SCL), reb A (REB), glucose (GLU, control), or sucrose (SUC). The NNS were administered in water at doses equivalent to the human acceptable daily intake (ADI). Following eight weeks, the GM composition in fecal samples was analyzed through 16S ribosomal RNA gene sequencing. The NNS did not modify the diversity, the structure, the composition at the phylum level, and the Firmicutes/Bacteroidetes (F/B) ratio of the GM. At the class level, REB with HFD decreased Bacilli and increased Faecalibacterium abundance. SCL and REB in combination with ND reduced the genera Romboutsia and Lactobacillus. On the other hand, the analysis of the effect of the diet without NNS showed that HFD increased the proportions of Bacilli and Coriobacteriia, despite not observing changes in the F/B ratio. Our study indicates that when sucralose or reb A is consumed at recommended doses, there is no observed alteration in the diversity and composition of the GM at the phylum level. Consequently, our data suggest that these NNS do not substantially impact the GM. We encourage further research to delve into the potential modifications of the GM at the level of specific bacterial taxa, as this could offer valuable insights for clinical interventions.
Litter Production and Decomposition in Agro-ecosystems of Aleta Chuko District of Sidama Region, Ethiopia
Authors: Argado Zenebe , Wele Ambachew , Amdie Tesfaye 
Published: February 2024

Abstract

Litter fall and decomposition in agro-ecosystems varies by tree species where indigenous and exotic species were mixed. In such diversified species, litter decomposition and nutrient dynamics are not well understood in Aleta Chuko. This study was aimed at measuring litter fall of seven abundant woody species in Coffee-Enset based agroforestry (CEA) and eucalyptus camaldulensis woodlots; determining decomposition rate and nutrient contents of leaf litter. Three replicates for each species which were distributed over three transect lines, were used for litter fall and in situ decomposition experiment for one year by using litter traps and nylon-mesh litter-bag respectively. Mean monthly litter fall ranges from 40.9 for M. indica to 79.7 g m− 2 for O. kenyensis in CEA while it was 54.8 g m− 2 for E. camaldulensis. There was a significant difference in litter production among studied species (P < 0.05). Also, there was a significant difference in decay constant among studied species (P < 0.05). Indigenous species such as C. arabica, C. africana and M. ferruginea showed greater value of decay constant as compared to exotic species such as E.camaldulensis, P.americana and M. indica. C. arabica showed fastest decomposition with decay constant of 2.4 while it was 1.1 for E.camaldulensis. There was a significant loss of initial litter Ca, K and Mg contents within annual decomposition for all studied species. Whereas loss-gain condition of P, Na and N contents vary by woody species. Further research is required on nutrient dynamics of diverse plant species; particularly on factors responsible for increase or decrease in nutrient contents.
Investigation of the Bioconversion of Urea with Modified Lignosulfonate Biomass as Slow-Release Urea in Aqueous Medium and Rumen Fluid
Authors: Seifdavati Jamal , Mohammadzadeh Qasem , Shayesteh Keivan , Pourbayramian Roghayeh 
Published: February 2024

Abstract
For producing a combination of urea with acetylated lignosulfonate in the pellet form, the urea release was studied under real conditions in the livestock rumen. Morphological, chemical, and mechanical features of slow-release urea combination with acetylated lignosulfonate and comparing it with lignosulfonate, acetylated lignosulfonate, and urea were studied. The produced pellets with weight percentages (10, 30, 50, and 65) showed the potential of acetylated lignosulfonate in water. For the study of the amount of nitrogen release, slow-release urea (a combination of 65 wt% of acetylated lignosulfonate with 35 wt% of urea) together with three other experimental ratio samples including control ration without urea, urea control supplement ration and Nitro-Za inside the livestock rumen were studied and measured during a period of 0.5 to 24 h. The changes range in Nitro-Za ammonia Nitrogen concentration and the slow-release substance are aligned and similar and the slow-release substance belonging to the urea ration combined with acetylated lignosulfonate showed better performance from the fourth hour. The study results showed positive results regarding supplementing the feed ration with slow-release urea compared to urea and comparable to Nitro-Za.
Saikogenin A improves ethanol-induced liver injury by targeting SIRT1 to modulate lipid metabolism
Authors: Wang Yufeng , Jiang Ming-zhu , Feng Ying , Wang Jing-xian , Xu Xiang , Liu Ze-gan , Li Tongfei , Ma Shi-nan , Guo Xing Rong , Du Shi-ming 
Published: February 2024

Abstract

Chronic alcohol consumption leads to a spectrum of liver disorders, including steatosis, inflammation, and fibrosis, collectively known as alcohol liver disease (ALD). Steatosis is a critical hallmark of ALD, making it an important target for therapeutic intervention. Saikosaponin A (SSa), a compound found in Radix Bupleuri, has previously shown promising hepatoprotective, anti-inflammatory, and antioxidant properties. However, its role in ALD remains understudied, with direct regulatory targets and mechanisms yet to be fully elucidated in vivo. Moreover, the instability of SSa in gastric juice raises questions about the efficacy of oral SSa solutions.
In this study, we employed cell-based screening models and a chronic-plus-binge ethanol-fed mouse model to investigate the protective mechanisms of SSa and its metabolite Saikogenin A (SGA), against ethanol-induced hepatocyte injury. Our RNA-seq analysis in mice unveiled that SSa primarily acts through the mTOR and PPAR-α signaling pathways in the liver. Biophysical assays and loss-of-function experiments confirmed that SGA directly binds to and modulates the activity of the SIRT1 protein, mitigating ethanol-induced cell injury via the SIRT1-mTOR-PPAR-α axis.
Furthermore, our liver-specific knockdown of SIRT1 in an ALD mouse model demonstrated that the action of the liver proteins affected by oral SSa solutions is mediated through SIRT1. Significantly, SGA displayed a superior safety profile for hepatocytes compared to SSa. Our findings highlight the role of SGA in binding to the SIRT1 protein and enhancing its activity, thereby regulating hepatic fatty acid oxidation and adipogenesis in ALD. This suggests that SGA holds promise as a potential therapeutic agent for ALD.
Tumor relapse-free survival prognosis related consistency between cancer tissue and adjacent normal tissue in drug repurposing for solid tumor via connectivity map
Authors: Hao Mingyue , Li Dandan , Qiao Yuanyuan , Xiong Ming , Li Jun , Ma Wei 
Published: February 2024

Abstract

Traditional drug discovery encounters challenges, including high costs, time-intensive processes, and inherent risks. Drug repurposing emerges as a compelling alternative strategy to identify new indications for investigational or approved drugs, circumventing these obstacles. Among the various drug repurposing methods, the Disease-specific Signature-based Connectivity Map (Cmap) approach is widely utilized. However, the commonly employed method for constructing disease-specific signatures, known as Differentially Expressed Genes (DEG), faces issues related to inconsistencies between dysregulated genes and the prognosis of genes in tumor tissue, as well as discrepancies in prognosis genes between tumor and normal tissues. In this study, we propose a novel approach, Prognosis Consistency Scoring (PCS), aimed at addressing these inconsistencies. PCS measures the consistency of gene prognosis between tumor and normal tissues by combining the Recurrence-Free Survival (RFS) prognosis power of genes in both contexts. Disease-specific signatures are then constructed based on PCS, and drug repurposing is performed using the Cmap and Lincs Unified Environment (CLUE). Validation of predicted drugs is conducted using data from DrugBank and ClinicalTrials databases. Our findings reveal that the aforementioned inconsistencies are pervasive. Compared to signatures based on DEGs, PCS-based signatures exhibit superior performance, identifying more drugs with higher prediction accuracy, as confirmed by DrugBank annotations. Notably, a significant proportion of predicted drugs without corresponding indications were subsequently validated in the ClinicalTrials database. Additionally, PCS-based signatures demonstrate elevated disease specificity and association with Drug Related Gene (DRG).