bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seasonal variation of surface water quality and streamflow in Rispana: A tributary of Ganges river, India
Authors: Pant Manish , Singh Jabrinder 
Published: February 2024

AbstractTo evaluate the seasonal changes in quality and quantity of river Rispana (a tributary of river Ganges—Dehradun region of India) water quality index (WQI) was calculated at four major locations by using the weighted arithmetic method. Sixteen samples in all were taken throughout the pre‐monsoon (April 2022), monsoon (July 2022), post‐monsoon (November 2022), and the Winter season (January 2023). The major parameters, namely, temperature, dissolved oxygen (DO), pH, electrical conductivity (EC), and total dissolved solids (TDS), E‐coliform, sulphate, total alkalinity (TA), total hardness (TH), and biochemical oxygen demand (BOD) were considered for calculation of WQI. The WQI varied from minimum 44.53 (good) at site 1 – Kairwaan Gaon during monsoon season to maximum 302.69 (unfit for drinking) at site 4 – Mothrowala during the pre‐monsoon period. In 75% samples quality of water was unfit for domestic purpose and the contributing factors are DO, BOD, and Coliform which exceeds permissible limit in all samples. The major factors attributed for decline in the Rispana water quality are discharge of sewage, commercial sites effluent, and urban runoff. Pre‐monsoon, monsoon, post‐monsoon, and winter had average total coliform levels of 1849.6, 2419.6, 2419.6, and 1546.25, respectively, in MPN units. The average value of E‐coli for the four season vary from 1306.8 to 1888.1 MPN. The pollution load (physico‐chemical parameters and bacteriological factors) increases as the river Rispana flows to downstream in urban locations. Streamflow was observed as very lean except monsoon season (~1500 cfs) due to more extraction of Rispana's fresh water in upstream and unplanned changes in its catchment basin; thus establishes an extensive decline in availability of fresh water sources (Domestic use) in downstream Dehradun city area. In monsoon season, more streamflow (rain water influx) maintains the WQI also in good‐moderate category. The land use/land cover – LULC analysis in Rispana basin area, depicted 71% enhancement in urbanization (24.16%–41.55%) takes place in 15 years’ span (2003–2017). The study establishes the potential causes (natural and anthropogenic) for Rispana deterioration and provides a baseline (quality index) for aquatic and ecological rejuvenation of its watershed by executing a proper management strategy with people participation. This work contributes discernments for effective water resource management to address the smart city objectives and sustainable development goal‐SDG:6, urban and municipal authorities must take cognizance for revival of this domestic water resource.
Analysis of blood composition by porcine breeding cycle
Authors: Lee Seongju , Lee Sang Chul , Jeon Yubyeol 
Published: February 2024

AbstractBackgroundPigs for biomedical research are administered commercial fluids made for human consumption when they receive intravenous (IV) fluid therapy. Although pigs and humans have slightly different bodily fluid compositions, the composition shift happens at the same rate as their rapid growth.ObjectiveThe study aimed to analyse the composition of porcine blood according to breeding cycle using a portable blood analyser and provides data for developing customized IV fluids for pigs.MethodsPigs were sorted 25, 50, 100 and 120 days after birth, and sows were classified into candidate, pregnant and farrowing groups. A blood sample was collected from the external jugular vein and analysed using the EPOC blood analysis system using haematological, biochemical and gas parameters.ResultsThere was no difference among pig groups by age, but hematocrit and haemoglobin amounts decreased in sows after farrowing, but their concentrations were higher as compared to pigs. Glucose gradually reduced as age increased in pigs and during pregnancy in sows.ConclusionThis study provided a comprehensive analysis of porcine blood composition by breeding cycle and highlighted the importance of glucose supplementation for IV fluid therapy in pigs.
Surgical treatment and outcome of haematomyelia with a traumatic cause in a dog and a cat
Authors: Guo Shanshan 
Published: February 2024

AbstractTwo surgically treated haematomyelia cases were documented. One dog and one cat were presented for acute progressive paraplegia following a fall from height incident.Neurological examinations suggested a L4–S3 myelopathy in both cases. Radiography and magnetic resonance imaging in both cases revealed no fracture or subluxation of the spine, but well‐defined intramedullary mass lesions in lower lumbar regions compatible with haemorrhage and haematoma formation. Exploratory surgeries were performed over the lesions. Dark‐red friable masses were removed via myelotomy. Histopathological examinations revealed organizing haematomas at the acute to subacute stage. Postoperatively, both cases improved and regained ambulation. The dog walked normally but remained urinary and faecal incontinent 9 months after the surgery. The cat was continent and ambulatory with a paraparetic gait 5 months after the surgery. In both cases, the outcomes and the patients’ quality of life were considered satisfactory by the owners.
Gentlemen, you can't fight in here. Or can you?: How cyberspace operations impact international security
Authors: Maesschalck Sam 
Published: February 2024

AbstractThe military now views cyberspace as a new warfare domain, with constant cyber operations potentially causing significant consequences. Internationally, countries are heavily involved in cyberspace, but international law lags behind this evolution, raising questions about its application and retaliation measures. This article investigates international law in cyberspace and cyber operations in warfare and terrorism, exploring recent calls for increased legislation. The impact of cyberspace nonregulation on international security is examined from both positive and negative perspectives. It argues that solving anonymity and attribution issues requires state collaboration, with an initial step of cooperation against cyber‐terrorism. The conclusion emphasizes the necessity of cyberspace regulation and legislation for international and national security, offering a starting point for discussion.
Klimawandel und Gesundheit
Authors: Augustin Jobst , Burkart Katrin , Endlicher Wilfried , Herrmann Alina , Jochner-Oette Susanne , Koppe Christina , Menzel Annette , Mücke Hans-Guido , Sauerborn Rainer 
Published: February 2024

ZusammenfassungDie Autorinnen und Autoren betrachten in diesem Kapitel mögliche direkte und indirekte gesundheitliche Auswirkungen des Klimawandels in Deutschland und die jeweils spezifischen Anpassungsmaßnahmen. Thematisiert werden besonders klimasensible Erkrankungen, die im Zusammenhang mit Hitze und Kälte, UV-Strahlung, Pollenflug, Luftverschmutzung und Infektionserregern stehen. Abschließend wird auf wichtige gesundheitliche Aspekte des Klimaschutzes verwiesen, vor allem auf gesundheitliche co-benefits und die Rolle von nachhaltigen, resilienten Gesundheitssystemen.
CircYthdc2 generates polypeptides through two translation strategies to facilitate virus escape
Authors: Zheng Weiwei , Wang Linchao , Geng Shang , Xu Tianjun 
Published: February 2024

AbstractIt is known that about 10 circular RNAs (circRNAs) can encode functional polypeptides in higher mammals. However, it is not clear whether the functional polypeptides that can be translated by circRNAs are only the products of the evolution of higher animals, or also widely exist in other lower organisms. In addition, it is also unclear whether the two ways of translating polypeptides using IRES and m6A in the one circRNA are exclusive or coexistent. Here, we discovered a novel circRNA derived from the 3′-5′ RNA helicase Ythdc2 (Ythdc2) gene in lower vertebrate fish, namely circYthdc2, which can translate into a 170 amino acid polypeptide (Ythdc2-170aa) through IRES sequence or m6A modification, and is involved in antiviral immune of fish. Moreover, SCRV infection can promote circYthdc2 translate Ythdc2-170aa. Then, we found that both Ythdc2-170aa and Ythdc2 can promote the degradation of STING by promoting the ubiquitination modification of K11 and K48 link of STING, and weaken the host’s antiviral innate immunity. Notably, when circYthdc2 is abundant, Ythdc2 preferentially degrades circYthdc2 and no longer promotes the degradation of STING. Further studies have shown that circYthdc2 is highly conserved from lower vertebrates to higher mammals, and human circYthdc2 can also encode the same polypeptide and play a similar function to that of fish circYthdc2. This discovery confirms for the first time that the ability of circRNA to encode functional proteins is evolutionarily conserved, and finds that the ways of polypeptide translation by the same circRNA were diverse, which is of great significance for further elucidating the function and evolution of circRNAs in vertebrates.
Multiplicity results for Hamiltonian systems with Neumann-type boundary conditions
Authors: Fonda Alessandro , Mamo Natnael Gezahegn , Obersnel Franco , Sfecci Andrea 
Published: February 2024

AbstractWe prove some multiplicity results for Neumann-type boundary value problems associated with a Hamiltonian system. Such a system can be seen as the weak coupling of two systems, the first of which has some periodicity properties in the Hamiltonian function, the second one presenting the existence of a well-ordered pair of lower/upper solutions.
Long-term functional outcomes in polytrauma: a fundamentally new approach is needed in prediction
Authors: Meakes Simone , Enninghorst Natalie , Weaver Natasha , Hardy Benjamin M. , Balogh Zsolt J. 
Published: February 2024

Abstract
Purpose
Modern trauma care has reduced mortality but poor long-term outcomes with low follow-up rates are common with limited recommendations for improvements. The aim of this study was to describe the impact of severe injury on the health-related quality of life, specifically characterise the non-responder population and to identify modifiable predictors of poorer outcomes.

Methods
Five-year (2012–2016) prospective cohort study was performed at a level 1 trauma centre. Baseline Short-Form Health Survey (SF36) was collected at admission, and at 6 and 12 months postinjury together with demographics, injury mechanism and severity, psychosocial wellbeing, and return to work capacity.

Results
Of the 306 consecutive patients [age 52 ± 17 years, male 72%, ISS 21 (17, 29), mortality 5%], 195 (64%) completed questionnaires at baseline, and at 12 months. Preinjury physical health scores were above the general population (53.1 vs. 50.3, p < 0.001) and mental health component was consistent with the population norms (51.7 vs. 52.9, p = 0.065). One year following injury, both physical health (13.2, 95% CI 14.8, 11.6) and mental health scores (6.0, 95% CI 8.1, 3.8) were significantly below age- and sex-adjusted preinjury baselines. Non-responders had similar ISS but with a lower admission GCS, and were more likely to be younger, and without comorbidities, employment, or university education.

Conclusion
Contrary to their better than population norm preinjury health status, polytrauma patients remain functionally impaired at least 1 year after injury. The identified high risk for non-responding group needs more focused efforts for follow-up. A fundamentally different approach is required in polytrauma research which identify modifiable predictors of poor long-term outcomes.
Frankfurter Konzept einer stationären Cochleaimplantat-Frührehabilitation
Authors: Bruschke Stefanie , Zeh Roland , Baumann Uwe , Helbig Silke , Stöver Timo 
Published: February 2024

Zusammenfassung
Hintergrund
Mit der im Jahr 2020 aktualisierten AWMF-Leitlinie zur Versorgung mit einem Cochleaimplantat (CI) wurde erstmals der gesamte Prozess einer CI-Versorgung definiert. In der vorliegenden Studie wurden die Machbarkeit und die Ergebnisse einer sehr frühen Rehabilitationsmaßnahme (Reha) untersucht.

Methodik
Es wurden 54 Patienten in die Interventionsgruppe (IG) eingeschlossen, bei der die Reha innerhalb von 14 (maximal 28) Tagen nach der Implantation eingeleitet wurde. In eine Kontrollgruppe (KG, n = 21) wurden Patienten mit deutlich längerer Wartezeit eingeschlossen. Neben dem Beginn und der Dauer der Reha wurde das mit CI erreichte Sprachverstehen zu verschiedenen Zeitpunkten innerhalb von 12 Monaten erfasst. Zusätzlich wurde mit Fragebögen der Aufwand der Anpassung des CI-Prozessors und die Zufriedenheit der Patienten mit dem Ergebnis sowie dem Zeitpunkt des Beginns der Reha ermittelt.

Ergebnisse
Die Wartezeit zwischen Implantation und Beginn der Reha lag in der IG bei 14 Tagen und in der KG bei 106 Tagen (Mediane). Es konnten 92,6 % der Patienten der IG die Reha innerhalb von 14 Tagen antreten. Der Effekt der Reha lag in der IG bei 35 und in der KG bei 25 Prozentpunkten (Freiburger Einsilbertest). Nach 6 und 12 Monaten (M) CI-Nutzung zeigten beide Gruppen sowohl in der Testbedingung in Ruhe (IG/KG 6M: 70 %/70 %; 12M: 70 %/60 %, Freiburger Einsilbertest) als auch im Störgeräusch (IG/KG 6M: −1,1 dB SNR/–0,85 dB SNR; 12M: −0,65 dB SNR/0,3 dB SNR, Oldenburger Satztest) vergleichbare Ergebnisse. Die mittels des Fragebogens Speech, Spatial and Qualities of Hearing Scale (SSQ) erfassten Ergebnisse für die Einschätzung der Hörqualität zeigten nach 6 Monaten eine bessere Bewertung in der IG, die sich nach 12 Monaten an die Ergebnisse der KG anglich. Die IG war mit dem Zeitpunkt des Beginns der Reha deutlich zufriedener als die KG. Alle anderen aus Fragebögen ermittelten Daten zeigten keine Unterschiede zwischen den beiden Gruppen.

Schlussfolgerung
Der sehr frühe Beginn einer stationären Reha nach Cochleaimplantation ist erfolgreich umsetzbar. Die Reha konnte innerhalb von 7 Wochen nach der Implantation abgeschlossen werden. Der Vergleich der Ergebnisse der Tests des Sprachverstehens vor und nach der Reha zeigte eine deutliche Steigerung. Somit ist ein deutlicher Reha-Effekt nachweisbar. Die Aufnahme der CI-Rehabilitation in den Katalog der Anschlussheilbehandlungen ist somit wissenschaftlich begründet und damit dringend zu empfehlen.
Feasibility of prevention of type 2 diabetes in low- and middle-income countries
Authors: Kengne Andre P. , Ramachandran Ambady 
Published: February 2024

AbstractType 2 diabetes is a leading cause of global mortality and morbidity. Nearly 80% of individuals with diabetes live in low- and middle-income countries (LMICs), where nearly half of those with the condition remain undiagnosed. The majority of known cases have sub-optimal clinical outcomes. Moreover, large populations with impaired glucose tolerance and/or impaired fasting glucose contribute to the rapid increase in type 2 diabetes. Globally, priority should be given to limit the population with diabetes, especially in LMICs, alongside actions to optimise the care of people diagnosed with diabetes. Primary prevention studies in LMICs have generated evidence to show the efficacy and scalability of strategies to fully prevent or delay the development of diabetes in high-risk groups. However, these are mainly limited to certain countries in Asia, particularly China and India. The studies have indicated that prevention policies are effective in populations with a high risk of type 2 diabetes, and they also have long-term benefits, not only for the risk of type 2 diabetes but also for the risk of associated metabolic disorders, such as CVDs. For the effective conduct of national programmes, innovative mechanisms must be implemented, such as the use of information technology, joint efforts of multiple teams implementing similar programmes, and involvement of governmental and non-governmental partnerships. Continuous monitoring and long-term studies are required to assess the utility of these programmes. The effectiveness of such programmes in LMICs has not been proven over the longer term, except in China. Despite the available evidence, the feasibility of prevention strategies for type 2 diabetes in LMICs at population level remains an enigma. There remain challenges in the form of cultural, societal and economic constraints; insufficient infrastructure and healthcare capacity; and the non-fully elucidated natural history and determinants of type 2 diabetes in LMICs.
Graphical Abstract
Carbamoylase-based impedimetric electronic tongue for rapid detection of paralytic shellfish toxins
Authors: Raposo Mariana , Soreto Silvia , Moreirinha Catarina , Gomes Maria Teresa S. R. , Costa Sara T. , Botelho Maria João , Melo Bruno M. G. , Costa Luís Cadillon , Rudnitskaya Alisa 
Published: February 2024

AbstractPhytotoxins produced by marine microalgae, such as paralytic shellfish toxins (PSTs), can accumulate in bivalve molluscs, representing a human health concern due to the life-threatening symptoms they cause. To avoid the commercialization of contaminated bivalves, monitoring programs were established in the EU. The purpose of this work is the implementation of a PST transforming enzyme—carbamoylase—in an impedimetric test for rapid simultaneous detection of several carbamate and N-sulfocarbamoyl PSTs. Carbamoylase hydrolyses carbamate and sulfocarbamoyl toxins, which may account for up to 90% of bivalve toxicity related to PSTs. Conformational changes of carbamoylase accompanying enzymatic reactions were probed by Fourier transform mid-infrared spectroscopy (FT-MIR) and electrochemical impedance spectroscopy (EIS). Furthermore, a combination of EIS with a metal electrode and a carbamoylase-based assay was employed to harness changes in the enzyme conformation and adsorption on the electrode surface during the enzymatic reaction as an analytical signal. After optimization of the working conditions, the developed impedimetric e-tongue could quantify N-sulfocarbamoyl toxins with a detection limit of 0.1 µM. The developed e-tongue allows the detection of these toxins at concentration levels observed in bivalves with PST toxicity close to the regulatory limit. The quantification of a sum of N-sulfocarbamoyl PSTs in naturally contaminated mussel extracts using the developed impedimetric e-tongue has been demonstrated.
Nitric oxide synthase expression in Pseudomonas koreensis MME3 improves plant growth promotion traits
Authors: Labarthe María M. , Maroniche Guillermo A. , Lamattina Lorenzo , Creus Cecilia M. 
Published: February 2024

Abstract
The development of novel biotechnologies that promote a better use of N to optimize crop yield is a central goal for sustainable agriculture. Phytostimulation, biofertilization, and bioprotection through the use of bio-inputs are promising technologies for this purpose. In this study, the plant growth–promoting rhizobacteria Pseudomonas koreensis MME3 was genetically modified to express a nitric oxide synthase of Synechococcus SyNOS, an atypical enzyme with a globin domain that converts nitric oxide to nitrate. A cassette for constitutive expression of synos was introduced as a single insertion into the genome of P. koreensis MME3 using a miniTn7 system. The resulting recombinant strain MME3:SyNOS showed improved growth, motility, and biofilm formation. The impact of MME3:SyNOS inoculation on Brachypodium distachyon growth and N uptake and use efficiencies under different N availability situations was analyzed, in comparison to the control strain MME3:c. After 35 days of inoculation, plants treated with MME3:SyNOS had a higher root dry weight, both under semi-hydroponic and greenhouse conditions. At harvest, both MME3:SyNOS and MME3:c increased N uptake and use efficiency of plants grown under low N soil. Our results indicate that synos expression is a valid strategy to boost the phytostimulatory capacity of plant-associated bacteria and improve the adaptability of plants to N deficiency.

Key points
• synos expression improves P. koreensis MME3 traits important for rhizospheric colonization
• B. distachyon inoculated with MME3:SyNOS shows improved root growth
• MME3 inoculation improves plant N uptake and use efficiencies in N-deficient soil
Can Exclusion of Feral Ecosystem Engineers Improve Coastal Floodplain Resilience to Climate Change? Insight from a Case Study in North East Arnhem Land, Australia
Authors: Sloane Daniel R. , Ens Emilie , Wunungmurra Yumutjin , Mununggurr Lanydjana , Falk Andrew , Wunungmurra Richard , Gumana Goninyal , Towler Gillian , Preece Dave ,   The Yirralka Rangers
Published: February 2024

AbstractGlobal climate change can interact with local drivers, such as ecosystem engineers, to exacerbate changes in ecosystem structure and function, with socio-ecological consequences. For regions of Indigenous interest, there may also be cultural consequences if species and areas affected are culturally significant. Here we describe a participatory approach between the Indigenous (Yolngu) Yirralka Rangers and non-Indigenous researchers that explored the interaction between sea level rise and feral ungulate ecosystem engineers on culturally significant floodplains in the Laynhapuy Indigenous Protected Area (IPA), northern Australia. A feral ungulate exclusion fence array (12 fenced and 12 unfenced plots) was stratified by elevation/salinity to disentangle the effects of salinity and ungulates on floodplain soil and vegetation. We found that exclusion of feral ungulates improved ground cover vegetation, which, according to our literature-derived ecosystem process model, may enhance soil trapping and reduce evapotranspiration to provide the antecedent conditions needed to improve floodplain resilience to sea level rise. The mid-zone of the supratidal floodplain study site was suggested as the region where the benefits of fencing were most pronounced after two years and ground cover species diversity was highest. Ongoing monitoring is required to investigate whether removal of feral ungulates can increase resilience against sea level rise and recruitment of eco-culturally significant Melaleuca species. An interview with a key Yolngu Traditional Owner of the study site demonstrated the importance and effectiveness of the partnership. Yolngu land owners and rangers were active co-researchers and will decide if, when and how to integrate results into feral ungulate management and climate adaptation responses, highlighting the importance of industry-university partnerships in maximising biocultural conservation outcomes.
Calibration of agent based models for monophasic and biphasic tumour growth using approximate Bayesian computation
Authors: Wang Xiaoyu , Jenner Adrianne L. , Salomone Robert , Warne David J. , Drovandi Christopher 
Published: February 2024

AbstractAgent-based models (ABMs) are readily used to capture the stochasticity in tumour evolution; however, these models are often challenging to validate with experimental measurements due to model complexity. The Voronoi cell-based model (VCBM) is an off-lattice agent-based model that captures individual cell shapes using a Voronoi tessellation and mimics the evolution of cancer cell proliferation and movement. Evidence suggests tumours can exhibit biphasic growth in vivo. To account for this phenomena, we extend the VCBM to capture the existence of two distinct growth phases. Prior work primarily focused on point estimation for the parameters without consideration of estimating uncertainty. In this paper, approximate Bayesian computation is employed to calibrate the model to in vivo measurements of breast, ovarian and pancreatic cancer. Our approach involves estimating the distribution of parameters that govern cancer cell proliferation and recovering outputs that match the experimental data. Our results show that the VCBM, and its biphasic extension, provides insight into tumour growth and quantifies uncertainty in the switching time between the two phases of the biphasic growth model. We find this approach enables precise estimates for the time taken for a daughter cell to become a mature cell. This allows us to propose future refinements to the model to improve accuracy, whilst also making conclusions about the differences in cancer cell characteristics.