bluuebunny/crossref_metadata_2025_split huggingface dataset:
Online collaborative tools for science education: Boosting learning outcomes, motivation, and engagement
Authors: Ateş Hüseyin , Köroğlu Mustafa 
Published: January 2024

AbstractBackgroundOnline collaboration tools have been identified as potentially effective means for enhancing student learning, motivation, and engagement in science education. However, their effectiveness in improving science education outcomes among middle school students remains uncertain.ObjectivesThe study aimed to investigate the impact of online collaboration tools on science education outcomes among middle school students, focusing on learning achievement, engagement, and motivation.MethodsA quasi‐experimental design with a pretest‐posttest control group was used in this study. A total of 60 eighth‐grade middle school students were involved in the study, with both the experimental and control groups comprising 30 students each. The experimental group incorporated the use of digital collaboration platforms, including Asana, Slack, and Team Viewer, as instrumental components of group project execution and discussion in their science lessons. Conversely, the control group adhered to the current educational approach in Turkey, characterized by the employment of an inquiry‐based learning strategy in their science instruction. Data was collected over eight weeks using a science achievement test, engagement scale, and science motivation scale.ResultsThe experimental group demonstrated significantly higher post‐test scores compared to the control group, suggesting that online collaboration tools positively impacted science education outcomes. Additionally, survey data indicated high levels of engagement and motivation among the experimental group students when using the online collaboration tools.ConclusionsThe findings support the notion that online collaboration tools can effectively enhance learning, motivation, and engagement in science education among middle school students. These results have significant implications for educators, educational institutions, policymakers, and curriculum developers. Further research is needed to examine the potential of these tools in various educational contexts and with different student populations. This will help broaden the understanding of how online collaboration tools can be integrated into diverse learning environments, potentially benefiting a larger number of students and further improving science education outcomes.
Exosome‐delivered miR‐410‐3p reverses epithelial‐mesenchymal transition, migration and invasion of trophoblasts in spontaneous abortion
Authors: Chen Zhen‐yue , Li Zhen , Zong Yun , Xia Bo , Luo Song‐ping , Deng Gao‐pi , Gao Jie 
Published: January 2024

AbstractCurrent studies have indicated that insufficient trophoblast epithelial‐mesenchymal transition (EMT), migration and invasion are crucial for spontaneous abortion (SA) occurrence and development. Exosomal miRNAs play significant roles in embryonic development and cellular communication. Hereon, we explored the roles of serum exosomes derived from SA patients on trophoblast EMT, migration and invasion. Exosomes were isolated from normal control (NC) patients with abortion for unplanned pregnancy and SA patients, then characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting. Exosomal miRNA profiles were identified by miRNA sequencing. The effects of serum exosomes on trophoblast migration and invasion were detected by scratch wound healing and transwell assays, and other potential mechanisms were revealed by quantitative real‐time PCR (RT‐PCR), western blotting and dual‐luciferase reporter assay. Finally, animal experiments were used to explore the effects of exosomal miR‐410‐3p on embryo absorption in mice. The serum exosomes from SA patients inhibited trophoblast EMT and reduced their migration and invasion ability in vitro. The miRNA sequencing showed that miR‐410‐3p was upregulated in SA serum exosomes. The functional experiments showed that SA serum exosomes restrained trophoblast EMT, migration and invasion by releasing miR‐410‐3p. Mechanistically, SA serum exosomal miR‐410‐3p inhibited trophoblast cell EMT, migration and invasion by targeting TNF receptor‐associated factor 6 (TRAF6) at the post‐transcriptional level. Besides, SA serum exosomal miR‐410‐3p inhibited the p38 MAPK signalling pathway by targeting TRAF6 in trophoblasts. Moreover, milk exosomes loaded with miR‐410‐3p mimic reached the maternal‐fetal interface and aggravated embryo absorption in female mice. Clinically, miR‐410‐3p and TRAF6 expression were abnormal and negatively correlated in the placental villi of SA patients. Our findings indicated that exosome‐derived miR‐410‐3p plays an important role between SA serum and trophoblasts in intercellular communication, suggesting a novel mechanism by which serum exosomal miRNA regulates trophoblasts in SA patients.
MicroRNA‐26a alleviates tubulointerstitial fibrosis in diabetic kidney disease by targeting PAR4
Authors: Qu Gaoting , Li Xingyue , Jin Ran , Guan Dian , Ji Jialing , Li Shanwen , Shi Huimin , Tong Pingfan , Gan Weihua , Zhang Aiqing 
Published: January 2024

AbstractOur previous study found that miR‐26a alleviates aldosterone‐induced tubulointerstitial fibrosis (TIF). However, the effect of miR‐26a on TIF in diabetic kidney disease (DKD) remains unclear. This study clarifies the role and possible mechanism of exogenous miR‐26a in controlling the progression of TIF in DKD models. Firstly, we showed that miR‐26a was markedly decreased in type 2 diabetic db/db mice and mouse tubular epithelial cells (mTECs) treated with high glucose (HG, 30 mM) using RT‐qPCR. We then used adeno‐associated virus carrying miR‐26a and adenovirus miR‐26a to enhance the expression of miR‐26a in vivo and in vitro. Overexpressing miR‐26a alleviated the TIF in db/db mice and the extracellular matrix (ECM) deposition in HG‐stimulated mTECs. These protective effects were caused by reducing expression of protease‐activated receptor 4 (PAR4), which involved in multiple pro‐fibrotic pathways. The rescue of PAR4 expression reversed the anti‐fibrosis activity of miR‐26a. We conclude that miR‐26a alleviates TIF in DKD models by directly targeting PAR4, which may provide a novel molecular strategy for DKD therapy.
Complex adaptive organisations: How three‐dimensional visualisations can help to understand their structures and behaviours
Authors: Sturmberg Joachim P. , Gainsford Len 
Published: January 2024

AbstractMany organisations struggle to achieve their true potential. In part it is a problem of organisational design, which is an outcome of a particularly common—command and control—leadership philosophy. The traditional linear hierarchical structure of organisations suggests that all knowledge and power concentrates at the top organisational layer, and that people in the lower layers need to be told what to do and when. These arrangements disempower people at the lower level, preventing them from providing the necessary feedback to pre‐emptively address emerging concerns. Systems and complexity thinking provide different ways of understanding organisations and their complex adaptive behaviours. In this paper, we first describe the key characteristics of complex adaptive organisations—their structure and dynamic behaviours—and then propose a three‐dimensional visualisation of a complex adaptive organisation that allows its members to clearly see and appreciate its interdependencies.
Latitudinal biodiversity gradients of rocky intertidal assemblages: Spatial scales and complex associations with environmental factors
Authors: Hadiyanto Hadiyanto , Prince Jane , Hovey Renae K. 
Published: January 2024

AbstractLatitudinal biodiversity gradients vary across taxonomic groups and spatial scales, and various environmental factors have been associated with those patterns. However, it remains unknown whether taxonomic groups with strong ecological associations have similar or different latitudinal patterns. Macroalgae are foundation assemblages on rocky intertidal shores and are often inhabited by invertebrates, predominantly polychaetes. This study analysed latitudinal patterns of macroalgae and their associated polychaetes at different spatial scales and determined how environmental factors influenced those patterns. Macroalgae and polychaetes were collected from transects within 38 rocky intertidal shores of Western Australia at 14 latitudes between 18° S and 34° S. Latitudinal gradients in species richness, diversity (Simpson's diversity index) and abundance were evaluated at transect, site and latitudinal scales. Relationships between environmental factors and rocky intertidal assemblages were analysed using piecewise structural equation modelling based on direct, indirect and complex models. Macroalgae showed increases in species richness, diversity and abundance at transect and site scales towards high latitudes, but species richness and diversity patterns were unclear at the latitudinal scale where transect and site data were pooled. In contrast, polychaete diversity decreased towards high latitudes, although this pattern was unclear at the transect scale. Polychaete richness and abundance tended to follow parabolic patterns that peaked at 27° S. Relationships between environmental factors and rocky intertidal assemblages were best described by a complex model, with significant relationships more often at transect and site scales. Sea surface temperatures showed the strongest relationship with macroalgal and polychaete distributions.
Incidence of invasive fungal diseases in inflammatory bowel disease patients: A nationwide study in South Korea
Authors: Na Ji Eun , Jung Sung Hoon , Choi Arum , Kim Sukil , Kim Tae‐Oh 
Published: January 2024

AbstractBackgroundLimited reports exist regarding invasive fungal diseases (IFDs) in inflammatory bowel disease (IBD) patients.ObjectivesThis study aims to investigate the incidence and risk factors of IFDs, specifically invasive candidiasis, aspergillosis and pneumocystosis, in IBD patients in South Korea using nationwide data.Patients/MethodsA population‐based retrospective cohort of 42,913 IBD patients between January 2010 and December 2018 was evaluated using the Health Insurance Review and Assessment database. The primary outcome was the incidence of IFDs, including invasive candidiasis, aspergillosis and pneumocystosis, while the secondary outcome involved analysing the risk factors associated with each specific infection.ResultsThe study included a total of 42,913 IBD patients, with 29,909 (69.7%) diagnosed with ulcerative colitis (UC) and 13,004 (30.3%) diagnosed with Crohn's disease (CD). IFDs occurred in 166 IBD patients (0.4%), with 93 cases in UC patients and 73 cases in CD patients. The incidence rates of invasive candidiasis, aspergillosis and pneumocystosis in IBD patients were 0.71 per 1000 person‐years (PYs), 0.15 per 1000 PYs and 0.12 per 1000 PYs, respectively. The cumulative incidence of invasive candidiasis (adjusted p‐value <.001) and Pneumocystosis (adjusted p‐value = .012) was found to be higher in CD patients than in UC patients. Each IFD had different risk factors, including IBD subtypes, age at diagnosis, anti‐tumour necrotic factor agents or the Charlson comorbidity index.ConclusionBased on nationwide data in South Korea, this study shows that IFDs occur consistently in patients with IBD, albeit with a low frequency.
Transmission of high arterial pressure into renal microvessels during venous‐clamping augments ischaemia/reperfusion‐induced acute kidney injury in anaesthetized rats
Authors: Maftoon‐Azad Mohammad Javad , Nazari Somayeh , Keshavarz Somayeh , Owji Seyed Mohammad , Moosavi Seyed Mostafa Shid 
Published: January 2024

AbstractAimIn two recent studies, we observed that a 30‐min renal vein clamping caused formation of interstitial haemorrhagic congestion in ischaemic and ischaemic/reperfused kidney along with the development of severer acute kidney injury (AKI) than renal artery or pedicle clamping. It was suggested that the transmission of high arterial pressure into renal microvessels during vein occlusion probably causes the occurrence of interstitial haemorrhagic congestion that augments AKI. The present investigation aimed to evaluate this suggestion by reducing renal perfusion pressure (RPP) during renal venous occlusion.MethodsAnaesthetized male Sprague–Dawley rats were divided into three groups (n = 8), which underwent a 2‐h reperfusion period following 30‐min bilateral renal venous clamping along with reduced RPP (VIR‐rRPP group) or without reduced RPP (VIR group) and an equivalent period after sham‐operation (Sham group).ResultsThe VIR‐rRPP group compared with VIR group had lower levels of kidney malondialdehyde and tissue damages as epithelial injuries of proximal tubule and thick ascending limb, vascular congestion, intratubular cast and oedema, along with the less reductions in renal blood flow, creatinine clearance, Na+‐reabsorption, K+ and urea excretion, urine osmolality and free‐water reabsorption. Importantly, the formation of intensive interstitial haemorrhagic congestion in the VIR group was not observed in the VIR‐rRPP group.ConclusionThese results indicate that the transmission of high arterial pressure into renal microvessels during venous occlusion leads to rupturing of their walls and the formation of interstitial haemorrhagic congestion, which has an augmenting impact on ischaemia/reperfusion‐induced renal structural damages and haemodynamic, excretory and urine‐concentrating dysfunctions.
The management of osteoradionecrosis in palliative head and neck cancer patients
Authors: AlHakim Reem , Jawad Nadeen , Sproat Chris , Patel Vinod 
Published: January 2024

AbstractObjectiveOsteoradionecrosis (ORN) is a well‐recognised complication of radiotherapy in the cured head and neck cancer (HNC) patients. More recently with improved survivorship in the palliative HNC group, there is an increasing population that is also burdened by radiation late effects such as ORN. Currently, there is no proven approach to manage ORN in this cohort of patients. This study reports the management and outcome of a case series of palliative care HNC patients with established ORN.Materials and MethodsThe clinical records of 20 palliative care HNC patients with an established ORN diagnosis were identified and reviewed retrospectively.ResultsAll 20 patients were managed via a conservative approach. Medical management via PENTO (n = 4, 20%) or PENTOCLO (n = 8, 40%) was the most popular approach. All but two patients had ORN that healed (4/20, 20%), or was stabilised (14/20, 70%). The two patients with progressive ORN had a tumour diagnosis adjacent to the necrotic site.ConclusionMedical management via PENTO/CLO provided promising results in this small cohort of palliative patients with ORN. The overall combined healing and stability of ORN status are comparable to those in the literature, though most cases are stabilised as opposed to cured.
Increased prevalence of metabolic syndrome and non‐alcoholic fatty liver disease in children with atopic dermatitis: A case–control study from northern India
Authors: Reddy Pratheeksha , Mahajan Rahul , Mehta Hitaishi , De Dipankar , Bhatia Anmol , Kumar Rakesh , Handa Sanjeev 
Published: January 2024

AbstractBackground/ObjectivesAtopic dermatitis (AD) is a chronic inflammatory skin disorder that affects children worldwide, with potential associations to metabolic syndrome (MetS) and non‐alcoholic fatty liver disease (NAFLD). Limited research exists on the interplay between AD, MetS, and NAFLD in the pediatric population. This study aimed to investigate the prevalence and potential relationships among AD, MetS, and NAFLD in children.MethodsA case–control study design was employed, recruiting 50 children with AD (median age: 9.5 years) and 50 age‐ and sex‐matched healthy controls (median age: 11.5 years, p = .051). Data on demographic characteristics, clinical features, disease severity, treatment history, anthropometric measurements, and laboratory evaluations were collected. MetS and NAFLD were diagnosed based on established criteria.ResultsThe prevalence of MetS was significantly higher in children with AD compared with controls (24% vs. 2%, p = .002). Significant differences for systolic blood pressure (p < .001), diastolic blood pressure (p = .012), and waist circumference (p = .040) were observed between AD patients and controls. Children with AD had higher triglyceride levels (p = .005). NAFLD was exclusively seen in moderate to severe AD cases (6% vs. 0%, p = .242). AD severity showed associations with increased body mass index (p = .020).ConclusionThis study highlights the increased prevalence of MetS and the potential association with NAFLD in children with AD. The findings suggest that AD may contribute to the development of metabolic abnormalities at an early age. Further research is needed to elucidate the underlying mechanisms and explore preventive strategies for these interconnected conditions.
Evolutionary studies of the basic helix–loop–helix (bHLH) IVc gene family in plants and the role of AtILR3 in Arabidopsis response to ABA stress
Authors: Jiang Min , Niu Yuqian , Wen Guosong , Zhao Changling , Gao Qing , Li Guiqiong 
Published: January 2024

AbstractThe basic helix–loop–helix (bHLH) IVc transcription factors (TFs) play central roles in controlling iron (Fe) homeostasis and biotic stress responses. However, their evolutions and functions in other abiotic stresses are poorly understood. In this study, the IAA‐LEUCINE RESISTANT3 (ILR3) homologs were traced roughly back to before the early origin of land plants and divided into six main clades (Clade A‐F). Further analysis found that the ILR3 orthologs were angiosperm‐specific, suffering from motif‐acquisition events and loose purifying selection. Synteny analysis displayed that the whole genome duplications (WGDs) contributed to the establishment of the IRON DEFICIENCY TOLERANT1 (IDT1, also called bHLH34)/bHLH104 lineage prior to the divergence of angiosperms. Sequence analysis revealed that the ILR3 homologs had some novel and conserved motifs except bHLH and leucine zipper (ZIP) domains. Particularly, Arabidopsis thaliana ILR3 (AtILR3) was a nuclear protein and greatly activated by ABA and CdCl2 stresses. Simultaneously, the molecular and genetic analyses suggested that the AtILR3 acted as a positive regulator in the ABA stress response through enhancing the ability of reactive oxygen species (ROS)‐scavenging, such as the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). The inductively coupled plasma mass spectrometry (ICP‐MS) analyses exhibited that the AtILR3 affected the absorption of nutrient elements, especially iron elements, under ABA stress. Collectively, our findings could shed deep light on the origin and evolution of the plant ILR3s, as well as the functions of the AtILR3 under ABA stress.
Genetic Interactions Between BEN1‐ and Cytochrome P450‐Mediated Brassinosteroid Inactivation
Authors: Tayengwa Reuben , Westenskow Shelby R. , Peng Hao , Hulbert Anna K. , Neff Michael M. 
Published: January 2024

AbstractBrassinosteroids (BRs) are essential plant growth‐promoting hormones involved in many processes throughout plant development, from seed germination to flowering time. Since BRs do not undergo long‐distance transport, cell‐ and tissue‐specific regulation of hormone levels involves both biosynthesis and inactivation. To date, ten BR‐inactivating enzymes, with at least five distinct biochemical activities, have been experimentally identified in the model plant Arabidopsis thaliana. Epigenetic interactions between T‐DNA insertion alleles and genetic linkage have hindered analysis of higher‐order null mutants in these genes. A previous study demonstrated that the bas1‐2 sob7‐1 ben1‐1 triple‐null mutant could not be characterized due to epigenetic interactions between the exonic T‐DNA insertions in bas1‐2 and sob7‐1, causing the intronic T‐DNA insertion of ben1‐1 to revert to a partial loss‐of‐function allele. We used CRISPR‐Cas9 genome editing to avoid this problem and generated the bas1‐2 sob7‐1 ben1‐3 triple‐null mutant. This triple‐null mutant resulted in an additive seedling long‐hypocotyl phenotype. We also uncovered a role for BEN1‐mediated BR‐inactivation in seedling cotyledon petiole elongation that was not observed in the single ben1‐2 null mutant but only in the absence of both BAS1 and SOB7. In addition, genetic analysis demonstrated that BEN1 does not contribute to the early‐flowering phenotype, which BAS1 and SOB7 redundantly regulate. Our results show that BAS1, BEN1, and SOB7 have overlapping and independent roles based on their differential spatiotemporal tissue expression patterns
Inversion formula for an integral geometry problem over surfaces of revolution
Authors: Ustaoglu Zekeriya 
Published: January 2024

AbstractAn integral geometry problem is considered on a family of ‐dimensional surfaces of revolution whose vertices lie on a hyperplane and directions of symmetry axes are fixed and orthogonal to this plane, in . More precisely, the reconstruction of a function , , , from the integrals of the form extended over a chosen side of all surfaces of revolution of a given family is investigated. Unlike the usual Radon transform, the integrals considered here are not taken with respect to the surface area element. A Fourier slice identity and a backprojection‐type inversion formula are obtained with a method based on the Fourier and Hankel transforms. The reconstruction procedure and some analytical and numerical implementations of the obtained inversion formulas in the cases of and are provided.
Performance evaluation of the Sysmex XN‐1000V in side‐by‐side comparison with the Siemens ADVIA 120 and manual methods for healthy CD Sprague–Dawley rats and CD‐1 mice
Authors: Schroeder Jill M. , Lee Hsiu‐Yung C. , Schultze Albert E. 
Published: January 2024

AbstractBackgroundThe Sysmex XN‐1000V automated hematology analyzer with multispecies software was released in June 2017 for use in research laboratories. Laser light, impedance, fluorescent staining, and fluorescent flow cytometry are used to analyze whole blood for CBC, reticulocyte counts, and WBC counts, including a 5‐part differential leukocyte analysis.ObjectivesA side‐by‐side comparison of the Sysmex XN‐1000V with the Siemens ADVIA 120 in analyzing blood from healthy mice and rats will provide insight into the performance of the new analyzer and its capabilities for use in drug development studies. Method correlation analyses on normal mouse and rat hematology data collected with both analyzers and manual reference methods will help determine the reliability of the data produced using the Sysmex XN‐1000V analyzer.MethodsWhole blood samples collected in K2EDTA from healthy CD‐1 mice and CD Sprague–Dawley rats were analyzed in parallel with the XN‐1000V and ADVIA 120 analyzers. Male and female mice, approximately 6–9 weeks old, and male and female rats, approximately 7–9 weeks old, were included in this study. Manual reference methods for WBC differential leukocyte analysis and packed cell volume (PCV) measurements were also performed. EP Evaluator version 11.2 (Data Innovations LLC, South Burlington, VT, USA) was used for method comparison statistical analysis.ResultsMost hematologic parameters for naïve mice and rats achieved correlation in the fair to excellent range, with the majority showing very good to excellent correlation with low biases (<11.0%) for cohorts analyzed separately and when cohort data were combined.ConclusionsThe Sysmex XN‐1000V Hematology Analyzer provided comparable results to those obtained from the Siemens ADVIA 120. We found the Sysmex XN‐1000V Hematology Analyzer to be acceptable for use in drug development studies for rats and mice.