bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enzyme kinetics of deoxyuridine triphosphatase from Western corn rootworm
Authors: Riera-Ruiz Carlos , Moriyama Hideaki 
Published: November 2023

Abstract
Objective
The Western corn rootworm (WCR), Diabrotica virgifera virgifera, is a highly adaptable insect pest that has evolved resistance to a variety of control strategies, including insecticides. Therefore, it is interesting to examine how housekeeping proteins in WCR have been changed under WCR-controlling strategies. In this study, we focused on one of such proteins in WCR, a ubiquitous enzyme 5'-triphosphate nucleotidohydrolase (dUTPase). In the thymidine synthetic pathway, dUTPase hydrolyzes deoxyuridine triphosphate (dUTP) and supplies the substrate, deoxyuridine monophosphate, for the thymidylate synthase (TS). It decreases the cellular content of uracil, reducing uracil misincorporation into DNA. Suppressing the dUTPase activity, therefore, contributes to thymineless death. In this study, we investigated the enzymatic properties of dUTPase.

Results
The WCR dUTPase gene (DUT) was synthesized with the addition of His-tag corresponding DNA sequence and then cloned and expressed in Escherichia coli, and the protein product was purified. The product of WCR DUT hydrolyzed dUTP and was designated as dUTPase. WCR dUTPase did not hydrolyze dATP, dTTP, dCTP, or dGTP. WCR dUTPase was analyzed via size-exclusion chromatography and exhibited a molecular weight corresponding to that of trimer. The present format can be interpreted as nuclear trimer type. Possible isomers will be examined once transcriptome analyses are conducted.
Heterogeneity of prostate-specific membrane antigen (PSMA) and PSMA-ligand uptake detection combining autoradiography and postoperative pathology in primary prostate cancer
Authors: Wang Hui , Remke Marianne , Horn Thomas , Schwamborn Kristina , Chen Yiyao , Steiger Katja , Weichert Wilko , Wester Hans-Jürgen , Schottelius Margret , Weber Wolfgang A. , Eiber Matthias 
Published: November 2023

Abstract
Background
Targeting prostate-specific membrane antigen (PSMA) has been highly successful for imaging and treatment of prostate cancer. However, heterogeneity in immunohistochemistry indicates limitations in the effect of imaging and radionuclide therapy of multifocal disease. 99mTc-PSMA-I&S is a γ-emitting probe, which can be used for intraoperative lesion detection and postsurgical autoradiography (ARG). We aimed to study its intraprostatic distribution and compared it with (immuno)-histopathology.

Results
Seventeen patients who underwent RGS between 11/2018 and 01/2020 with a total of 4660 grids were included in the preliminary analysis. Marked intratumor and intra-patient heterogeneity of PSMA expression was detected, and PSMA negative foci were observed in all samples (100%). Heterogeneous intra-patient PSMA-ligand uptake was observed, and no significant correlation was present between the degree of heterogeneity of PSMA expression and PSMA-ligand uptake. Higher PSMA-ligand uptake was observed in GS ≥ 8 than GS < 8 (p < 0.001). The appearance of Gleason Pattern (GP) 4 was strongly associated with higher uptake (coefficient: 0.43, p < 0.001), while GP 5 also affected the uptake (coefficient: 0.07, p < 0.001).

Conclusion
PSMA expression and PSMA-ligand uptake show marked heterogeneity. Prostate carcinoma with GP 4 showed significantly higher uptake compared with non-neoplastic prostate tissue. Our analyses extend the scope of applications of radiolabeled PSMA-ligands to ARG for identifying high-grade disease and using its signal as a noninvasive biomarker in prostate cancer.
Trends in Israeli community-based opioid prescribing, 2010–2020, an observational study of the country’s largest HMO
Authors: Dressler Reuven L. , Kaliner Ehud , Cohen Matan J. 
Published: November 2023

Abstract
Background
Prescription opioids are widely used for pain control and palliative care but have been associated with a variety of untoward effects, including opioid use disorder, addiction, and increased mortality. Patterns of opioid use in Israel are to date poorly described.

Methods
Using a community-based database, the authors performed a retrospective analysis of filled opioid prescriptions of Israeli HMO members 18 years of age or older during the years of 2010–2020 that filled at least one opioid prescription. Morphine milligram equivalent (MME) calculations were stratified by presence or absence of oncology diagnosis and by specific opioid medication.

Results
The percentage of HMO members who filled at least one opioid prescription increased every year from 2.1% in 2010 to 4.2% in 2020. There was an increase in the MME per prescription (44.2%), daily MME per capita (142.1%) and MME per prescription-filling patient (39%) from 2010 to 2020. Increased prescription opioid use is driven by a small group of non-oncological patients, which is less than 1.5% of opioid-prescribed patients and 0.1% of the adult population, primarily owing to fentanyl use.

Conclusion
Supervision and control of opioid prescriptions in Israel should be a focused effort directed at patients prescribed uniquely high dosages rather than a population-wide strategy that focuses on all patients prescribed opioids. This should be complemented by improved physician training and access to non-opioid therapies, as well as improved data collection and analysis.
Associations between nature exposure, screen use, and parent–child relations: a scoping review protocol
Authors: Torjinski Marina , Horwood Sharon 
Published: November 2023

Abstract
Background
Today’s youth are growing up in an evolving digital world, and concerns about the potential detrimental effects of excessive screen use on biopsychosocial outcomes in childhood are mounting. Parents worry about the impacts of screen-use on their children’s wellbeing but at the same time frequently fail to meet their own ideal screen time limits regarding their children’s screen use. There is an opportunity to shift research focus away from inflexible and often unrealistic childhood screen time guidelines towards exploration of positive parenting strategies that may have multiple beneficial and significant effects on children’s screen-related outcomes. An emerging body of literature suggests that screen time and nature exposure act on psychosocial outcomes in contrasting ways. There is evidence to suggest that exposure to natural environments may counteract some of the potential negative psychosocial effects of excessive screen use; however, this relationship is poorly understood. The overarching aim of this scoping review is to source, categorise, and synthesise existing research exploring the associations between nature exposure, screen use, and parenting across childhood.

Methods
This mixed-methods systematic scoping review will be conducted following Arksey and O’Malley’s framework with methodological enhancements from Levac and associates and recommendations from the Joanna Briggs Institute’s methodological guidance for conducting scoping reviews. Five electronic databases will be searched from August 2022 onwards. Two reviewers will independently screen titles, abstracts, and full-text articles. Peer reviewed articles related to the constructs of nature exposure, screen use, and parent/child relations will be considered in the context of early to late childhood. Study characteristics will be collated using a data charting tool collaboratively developed by the research team. Evidence will be presented using tabular and textual form and described using qualitative thematic analysis.

Discussion
This review will gather information about how key definitions are conceptualised, defined, and measured across the literature and map existing trends and areas for future research. It is intended that this review will inform and guide future research direction, recommendations, and programs aimed at supporting parents to navigate the challenges of parenting in a digital age.

OSF registration
https://doi.org/10.17605/OSF.IO/TFZDV
Mode of HIV exposure and excess burden of neurocognitive impairment in people living with HIV: a protocol for systematic review and meta-analysis of controlled studies
Authors: Ayu Astri Parawita , Rahadi Arie , Kristian Kevin , Sani Tara Puspitarini , Putra Aditya , Halim Glenardi , Mangkuliguna Ghea , Kusumoputri Theresia Puspoarum , Turana Yuda 
Published: November 2023

Abstract
Background
Chronic HIV infection significantly elevates the risk of brain pathology, precipitating neurocognitive impairment (NCI) among people living with HIV (PLWH). The diagnosis of NCI in PLWH hinges on evaluating deviations in neuropsychological test performance in comparison to HIV-seronegative normative controls. However, the adverse psychosocial conditions experienced by PLWH can also result in reduced test performance, potentially confounding the accurate NCI attribution to HIV infection. This planned systematic review aims to investigate potential disparities in the excess burden of NCI among PLWH in two groups of studies: (a) studies enrolling controls who shared a similar mode of HIV exposure (MoHE) with the PLWH participants (MoHE-adjusted) and (b) studies enrolling normative controls or controls without undefined MoHE (MoHE-naive).

Methods
We will systematically search five electronic databases (MEDLINE, Embase, PsycINFO, Web of Science, ProQuest) and registries (OpenGrey, ClinicalTrials.gov, ISRCTN registry). Studies reporting NCI in PLWH and HIV-seronegative controls with cross-sectional or baseline measurements, published from January 2007 to September 2023, will be included. To be classified as MoHE adjusted, a study must evidence ≥ 90% enrolment of both PLWH and their seronegative controls from the same MoHE group (e.g. men who have sex with men, people who use drugs or alcohol). Reports of test performance scores will be transformed into NCI proportions using simulated score distributions, applying a global deficit score cut-off ≥ 0.5 to estimate NCI cases.
The Newcastle–Ottawa scale adapted to the purpose of the review will be used to appraise study quality. Random-effects meta-analysis will be used to pool the excess burden of NCI in prevalence ratios and test the difference between MoHE-adjusted and MoHE-naive studies. Furthermore, subgroup analyses and meta-regression will be undertaken across categorical study-level covariates (e.g. study locations, NCI diagnostic criteria) and continuous/ordinal covariates (nadir CD4, number of neurocognitive domains assessed), respectively.

Discussion
This systematic review will contribute towards a greater appreciation of the unique psychosocial conditions of PLWH that are missing from the current case definition of HIV-associated neurocognitive disorder. The findings will additionally highlight possible disparities in the distribution of the excess burden of NCI by MoHE groups, thereby guiding the prioritization of mitigation efforts.

Systematic review registration
PROSPERO CRD42021271358
NuWa: off-state tolerant backscattering system with uncontrolled excitation traffics
Authors: Yang Zhiyi , He Xin , Lin Guiping , Jiang Weiwei , Zhu Yujun , Sun Jianfeng , Xu Yong , Yang Panlong 
Published: November 2023

AbstractBackscatter communication relies on passive reflections of the existing radio frequency (RF) signals, making it suitable for low-power and low-complexity communication in IoT applications. However, the performance of existing systems severely degrades in real-life environments, due to irregular “on” and “off” states of ambient signals like WiFi, which are not controllable. In this paper, we propose a joint coding and framing scheme for the backscattering physical layer to fight against the off-state in the excitation signal. We first design transmission schemes including both the Reed-Solomon (RS) codes and the frame structure, to correct the burst error caused by the off states. In order to implement the codes at the resource-constrained tag, we design a look-up table for the encoding process. We prototype our system NuWa that could efficiently backscatter with uncontrolled traffics generated randomly. We demonstrate that NuWa could achieve a 1 Mbps transmission throughput when the tag is over 1 m away from the receiver in high traffic load and 150 kbps in low traffic load. Finally, we evaluate the throughput with respect to the distance change between the tag and the receiver, and 950 kbps is achieved at a distance of $$6\,m$$

6

m

.
The Spanish language version of the TAPS tool: protocol for a validation and implementation study in primary care
Authors: Gryczynski Jan , Sanchez Katherine , Carswell Steven B. , Schwartz Robert P. 
Published: November 2023

Abstract
Background
The TAPS Tool (“Tobacco, Alcohol, Prescription drug, and illicit Substance use”) is a screening and brief assessment for detecting unhealthy substance use in healthcare settings that was developed by the National Institute on Drug Abuse Clinical Trials Network and validated in a multisite study. Our team developed a Spanish language version of the TAPS Tool that supports provider- and self-administration screening using a mobile/web-based platform, the TAPS Electronic Spanish Platform (TAPS-ESP).

Methods
This article describes the protocol and rationale for a study to validate the TAPS-ESP in a sample of Spanish-speaking primary care patients recruited from a network of community-based clinics in Texas (target N = 1,000). The TAPS-ESP will be validated against established substance use disorder diagnostic measures, alternative screening tools, and substance use biomarkers. The study will subsequently examine barriers and facilitators to screening with the TAPS-ESP from a provider workflow perspective using qualitative interviews with providers.

Discussion
Validating a Spanish language version of the TAPS Tool could expand access to evidence-based, linguistically accurate, and culturally relevant substance use screening and brief assessment for an underserved health disparity population.
Trial registration: The study was registered with www.clinicaltrials.gov: NCT05476588, 07/22/2022.
Review of surgical treatment of iatrogenic iliofemoral artery injury in the pediatric population after catheterization
Authors: Lozano-Corona Rodrigo , Torres-Machorro Adriana , Ortiz-Beitz Reinhard , Reyes-Monroy Aristeo , García-Lugo Ignacio , Ruben-Castillo Christopher , Guerrero-Galindo Luis Angel 
Published: November 2023

AbstractTrauma is the leading cause of death in the pediatric population. Although vascular trauma has an incidence of 6% in civilian population, iatrogenic injuries are the leading cause, and the most frequent injured vessel is the iliofemoral sector. However, little information is available and there are no guidelines about its treatment. Therefore, this review aimed to describe the information available concerning pediatric iatrogenic arterial trauma, focusing on the iliofemoral segment and present 3 cases. We described 11 articles with 171 patients, of whom 61% underwent surgery to treat iatrogenic trauma. Mean age was 3.28 years (standard deviation of 3.5 years), and 54% were female. Most iliofemoral injuries occurred after arterial catheterization for hemodynamic monitorization and therapeutic or diagnostic cardiac catheterization (due to congenital heart diseases, including septal defects, tetralogy of Fallot, aortic coarctation, and patent ductus arteriosus). For acute complications, arterial thrombosis was the leading injury, followed by pseudoaneurysm, hematoma, dissection, transection, avulsion, eversion, and combined lesions.
Can women’s empowerment in livestock farming improve household food security? Empirical evidence from rural households in Malawi
Authors: Mataka Tamala , Kaitibie Simeon , Ratna Nazmun N. 
Published: November 2023

Abstract
Background
Over the years the Government of Malawi has launched several initiatives to improve food security in the country. Despite these efforts there have been limited improvements in food security, raising the spectre of persistently elevated levels of food insecurity. Efforts to reduce food insecurity may involve women who play a central role in the production, processing, preparation and control of vital livestock products for food security. However, women’s ability to achieve food security is limited by their lack of access to productive resources and limited ability to participate in decision-making. The main objective of this study is to estimate the impact of women’s empowerment in household livestock production and marketing decisions on household food security.

Methods
This study uses data from a proportionate random sample of 400 households in two major livestock producing Extension Planning Areas in the Nsanje district to estimate the relationship between women’s empowerment and household food security in rural households in Malawi.

Results
Tobit regression results show that the empowerment of women in the livestock sector, especially in decisions pertaining to agricultural production, nutrition, and income control, improves household food security. In addition, factors such as household income, household size, and the main occupation of the household head play a significant role in ensuring household food security.

Conclusion
The results suggest that nutrition-sensitive programmes should target women’s agency in livestock production and nutrition decisions for improved food security among rural households in Malawi. As part of their food security strategy, Government of Malawi could develop programs to sensitize households on the importance of enhancing women’s agency in agriculture and nutrition decision-making, while also providing targeted income-support for women.
Pivoting injury prevention efforts during a pandemic: results of an international survey
Authors: Charyk Stewart Tanya , Unni Purnima , Hanson Holly Renee , Gilliland Jason , Clark Andrew , Fraser Douglas D. 
Published: November 2023

AbstractBackgroundThe COVID-19 a pandemic changed the world. Public health directives to socially distance with stay-at-home orders altered injury risk factor exposure, resulting injury patterns and conducting injury prevention (IP). The objective of this study was to determine the impact the COVID-19 pandemic on injury and IP at North American trauma centers (TC).ResultsSixty-two responses were received from pediatric (44%), adult (11%), and combined (31%) TC, from 22 American states, 5 Canadian provinces and Australia. The majority (91%) of programs targeted age groups from birth to 15 years old. Nearly one-third reported IP to be less of an institutional priority with funding redistributed in 15% of centers [median (IQR) − 25% (− 43, 1)], and resultant staffing changes at 38% of centers. A decrease in IP efforts was reported at 64% of TC. Overall, the majority of respondents reviewed injury data, with the top reported increased mechanisms mainly intentional: Firearm-related (75%), assaults (72%), and abuse (71%). Leading increased unintentional injuries were injuries occurring in the home such as falls (70%), followed by ATV (62%), and cycling (57%). Sites pivoted by presenting (74%) or participating (73%) in IP education virtually, social media posts (61%) and the addition of technology (29%). Top barriers were redeployment of partners (45%) and staff (31%), as well as lack of technology (40%) in the target population. Facilitators were technology at TC (74%), support of trauma program (63%), and having IP funding maintained (55%).ConclusionsNearly two-thirds of TC decreased IP efforts during the pandemic due to staffing and funding reductions. The leading reported increased injuries were intentional, indicating that violence prevention is needed, along with support for mental health. While TC successfully pivoted by using technology, access issues in the target population was a barrier resulting in health inequities.
Assessment HOMA as a predictor for new onset diabetes mellitus and diabetic complications in non-diabetic adults: a KoGES prospective cohort study
Authors: Lee Jibeom , Kim Moon-hyun , Jang Ji-Yong , Oh Chang-Myung 
Published: November 2023

Abstract
Background
Homeostasis model assessment for insulin resistance (HOMA-IR) is a biomarker for type 2 diabetes mellitus (T2DM). However, the role of HOMA-IR in the non-diabetic is unclear. This study aimed to determine whether IR measured HOMA-IR value is associated with new onset diabetes as well as vascular disease and can be used as an early predictor for diabetes and vascular diseases in non-diabetic participants.

Methods
From a prospective community-based cohort of 10,030 individuals, 4314 individuals younger than 65 years and without diabetes were enrolled and divided into three groups by baseline HOMA-IR tertiles: low (n = 1454), moderate (n = 1414), and high (n = 1446). The primary outcome was new onset T2DM. Secondary outcomes were chronic kidney disease (CKD) and a composite of coronary artery disease, myocardial infarction, and stroke as macrovascular events.

Results
The mean age was 51 years. The prevalence of hypertension and cholesterol and HbA1c were higher in the high HOMA-IR group. New onset T2DM (5.8%) and CKD (12.2%) incidence in the high HOMA-IR group was higher than that in the others. The prevalence of macrovascular events did not differ among groups. High-HOMA-IR was an independent risk factor for new onset T2DM (odds ratio 1.86 [1.17–2.96]; p = 0.01) and CKD (1.49 [1.12–1.98]; p = 0.01).

Conclusions
High HOMA-IR was an early predictor of new onset T2DM and CKD, regardless of HbA1c in non-diabetic individuals. Further research on the specific cut off value will be needed.
Usefulness of the skin-wrinkling test in a patient with probable small fiber neuropathy and Gaucher disease
Authors: Ducci Renata Dal-Prá , Ribas Michelle Zonkowski , Martinazzo Enzo Oku , Fustes Otto Jesus Hernandez , Lorenzoni Paulo José , Kay Claudia Suemi Kamoi , Scola Rosana Hermínia 
Published: November 2023

Abstract
Background
Gaucher disease (GD) is an autosomal recessive disease characterized by an inborn metabolic error. Although pain attributed to skeletal involvement is one of the most frequent symptoms of this condition, some patients may have neuropathic pain. Therefore, recent studies have analyzed the occurrence of peripheral polyneuropathy in patients with GD. In these studies, in cases when normal neurological examination and electroneuromyography were found, small fiber neuropathy (SFN) was investigated, mainly using skin biopsy and quantitative sensory testing.

Case presentation
We demonstrate the usefulness of the skin-wrinkling test performed by immersion in water for the evaluation of SFN in a 27-year-old woman with GD who presented with neuropathic pain. After excluding other causes, the main hypothesis was an association between SFN and GD, which was recently described in the literature and possibly underdiagnosed.

Conclusions
Although only skin biopsy can confirm the diagnosis of SFN, the skin-wrinkling test can be useful for the complimentary assessment of pain in patients with GD, owing to its easy application and wide availability.