bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cassava (Manihot esculenta Crantz)—A potential source of phytochemicals, food, and nutrition—An updated review
Authors: Scaria Shilpa Susan , Balasubramanian Balamuralikrishnan , Meyyazhagan Arun , Gangwar Jaya , Jaison Juhi Puthukulangara , Kurian Jissa Theresa , Pushparaj Karthika , Pappuswamy Manikanatan , Park Sungkwon , Joseph Kadanthottu Sebastian 
Published: January 2024

AbstractCassava (Manihot esculenta Crantz) is believed to be an important staple food crop providing potential valuable food source as well as variety of phytoconstituents. Its starchy tubers provide a significant source of energy for around 500 million individuals. Among staple crops, it is regarded to be one of the top suppliers of carbohydrates. Its physicochemical qualities, as well as its availability, have made it a captivating food component. Cassava starch is a valuable raw material used to make a variety of both native and modified starch for cooking purposes. They have also been used for a variety of industrial uses. Cassava starch and flour have the potential to be valuable alternatives to rice, maize, and wheat crops. The advantages included being a staple diet for humans, a component of animal feeds, a raw ingredient for food processing, edible coatings, locally produced alcoholic beverages, and ethanol manufacturing. The roots consist of cyanogenic glycosides, which can lead to lethal cyanide poisoning if tubers arse not properly detoxified using different processing methods include washing, fermentation, boiling, peeling and chemical processing to escape toxin content. The current review summarizes cassava's bioactive components which could be a potential source of various pharmaceutical drugs as well as a source of traditional and modern food applications.
Protective effect of secretory APE1/Ref‐1 on doxorubicin‐induced cardiotoxicity via suppression of ROS and p53 pathway
Authors: An Soo Yeon , Jin Seon‐Ah , Seo Hee Jeong , Lee Yu Ran , Kim Sungmin , Jeon Byeong Hwa , Jeong Jin‐Ok 
Published: January 2024

AbstractAimsThe clinical application of doxorubicin (DOX), a potent anthracycline anticancer drug that effectively treats various malignancies, is limited by its side effects, such as cardiomyopathy. Apurinic/apyrimidinic endonuclease 1/redox factor‐1 (APE1/Ref‐1) is a multifunctional protein that can be secreted and is a promising target for the reduction of DOX‐induced inflammation and oxidative stress. We aimed to investigate the protective role of secretory APE1/Ref‐1 against DOX‐induced cardiac injury.Methods and resultsDesignated adenoviral preprotrypsin‐leading sequence APE1/Ref‐1 (Ad‐PPTLS‐APE1/Ref‐1) was used to overexpress secretory APE1/Ref‐1 and assess its role in preventing DOX‐induced cardiomyopathy in vitro. Our findings revealed that exposure to secretory APE1/Ref‐1 significantly decreased N‐terminal pro‐B‐type natriuretic peptide levels in DOX‐treated H9C2 cells. In addition, secretory APE1/Ref‐1 reduced the severity of cardiomyocyte injury and apoptosis in both in vitro and in vivo DOX‐induced cardiotoxicity models. The observed cardioprotective effects of secretory APE1/Ref‐1 were mediated via inhibition of the p53 signalling pathway and enhancement of cell viability through attenuation of oxidative stress in DOX‐treated cardiomyocytes.ConclusionsOur study provides evidence that secretory APE1/Ref‐1 has the potential to inhibit DOX‐induced cardiac toxicity by inhibiting oxidative stress and p53 related apoptosis both in vitro and in vivo. These findings suggest that secretory APE1/Ref‐1 supplementation is a promising strategy to attenuate DOX‐induced cardiomyocyte damage in a preclinical model. Further clinical investigations are essential to validate the therapeutic efficacy and safety of the intervention in human subjects.
A comparison of neuromechanical adjustments to traditional and eccentric load reduction weighted jump squats
Authors: Zhang Liang , Yan Bing , Li Nan , Cai Yanni , Wang Jiale , Zhao Xinrong , Girard Olivier 
Published: January 2024

AbstractWe compared neuromechanical adjustments between traditional and eccentric load reduction weighted jump squats (WJSs). On separate visits, sixteen strength‐trained men performed three sets of six consecutive WJS with 0% (traditional WJS) or 50% eccentric loading reduction with a mechanical braking unit, while concentric load was set at 30% of 1 RM in all trials. A force platform and a motion capture system were used to assess neuromechanical parameters during eccentric and concentric phases of WJS. Peak power output (+4.5 ± 4.1%; p < 0.001), maximal concentric velocity (+3.2 ± 3.6%; p = 0.004), jump height (+19.6 ± 17.4%; p < 0.001), and reactive strength index (+28.2 ± 20.0%; p < 0.001) were higher when eccentric load was reduced by 50% versus 0%. Compared with traditional jump squat, eccentric phase duration (−6.7 ± 9.9%; p = 0.014), contact time (−6.3 ± 7.2%; p = 0.004), and time to reach peak power output (−7.8 ± 7.6%; p < 0.001) were shorter, while peak vertical force (−15.2 ± 17.5%; p = 0.005) and eccentric mechanical work (−23.9 ± 6.6%; p < 0.001) were lower with eccentric load reduction. Eccentric depth (p = 0.613), eccentric velocity (p = 0.070), amortization time (p = 0.060), maximal concentric force (p = 0.727), and concentric mechanical work (p = 0.396) did not differ significantly between conditions. Compared with traditional isoinertial loading, eccentric load reduction promoted more favorable neuromechanical adjustments during landing and propulsion phases in turn maximizing WJS performance.
Enhanced hydrogen storage capacity of graphene oxide through doping with copper ferrite nanoparticles
Authors: Hammoodi Omar G. , Mohammed Israa K. , Majeed Abdulwahhab H. , Alheety Mustafa A. , Mohammed Leqaa A. , Yahya M. Z. A. 
Published: January 2024

AbstractThe aim of this study was to prepare and characterize nanocomposites consisting of graphene oxide (GO) and copper ferrite (CuFe2O4) in different doping ratios (1%‐5%) and investigate their potential for storing hydrogen gas. The prepared nanocomposites were characterized using FTIR, XRD, SEM, and TEM. The results showed that hydrogen storage at 20°C was not possible with GO alone, but the incorporation of different proportions of copper ferrite nanoparticles significantly improved the hydrogen storage properties of the composite materials. The optimal percentage of copper ferrite was found to be around 3%, and it was observed that the percentage of ferrite added is crucial in determining the hydrogen storage properties of the nanocomposites. The best hydrogen storage value of 3.3% was achieved at a temperature of 20°C and a pressure of 80 bar within just 60 seconds. The adsorption of the composite material was found to be of the second type, with a re‐creation coefficient greater than 0.97 for all the materials tested, indicating favorable adsorption. The b value was up to 0, further confirming the favorable adsorption. Overall, the results demonstrate that the incorporation of copper ferrite nanoparticles can significantly improve the hydrogen storage properties of graphene oxide, and careful selection of the doping ratio is necessary to achieve the optimal performance. These findings have potential implications for the development of efficient hydrogen storage materials for clean energy applications.
Understanding the impact of socioeconomic and health factors on geriatric depression: A comparative study in rural and urban Bangladesh
Authors: Hossain Mohammad Kamal , Islam Md. Nazrul , Uddin Mohammed Taj , Hossain Md Sabbir 
Published: January 2024

AbstractBackground and AimsThe prevalence of depression among the elderly is a growing concern, and this study examines the differences between urban and rural areas in terms of geriatric depression.MethodsUsing a two‐stage random sampling approach in urban areas and a multistage random sampling approach in rural areas, the study surveyed 944 elderly individuals of both sexes.ResultsThe results indicate that the prevalence of depression was high, with 52.5% of the elderly population experiencing mild to severe depression. The study found that increasing age, female gender, nuclear family structure, and involvement of housewives or others were significant factors affecting depression in urban areas, while increasing age and elderly people without spouses were significant factors in rural areas. Additionally, the study identified hearing impairment, asthma, and arthritis as risk factors for depression in rural areas, and bronchitis, heart disease, and thyroid illness as significant factors in urban areas.ConclusionThese findings highlight the need for policymakers to focus on addressing the mental health needs of older people, particularly women and those without spouses.
Sustainability of medicinal animal products: Tokay geckos and pangolin scales as traditional Chinese medicine
Authors: Wang Yifu , Dufour Pauline C. , Yeung Kit Yi , Lo Sum Yi , Cheung Cherry Cheuk Yiu , Dingle Caroline , Bonebrake Timothy C. , Mumby Hannah S. 
Published: January 2024

AbstractTraditional Chinese Medicine (TCM) utilizes animal products derived from hundreds of species. For some species, such as the pangolins (family Manidae), demand from the medicinal trade has been recognized as a major threat and is under strict regulations to conserve declining endangered populations. In parallel, species such as the tokay gecko (Gekko gecko and Gekko reevesii) have only recently attracted conservation attention due to the high volumes in trade despite an apparently still abundant wild population. In this study, we collected market data to assess the trade in pangolin (illegal trade) and tokay gecko (legal trade) medicinal products. We also collect general public's attitude toward the trade and their understanding on the medicinal use of high‐profile endangered species (pangolins) and less well‐known species (tokay gecko) in Hong Kong. We interviewed a total of 169 shops/clinics and 153 members of the public. We found that tokay gecko was sold in 59% of the surveyed outlets. We estimate that trade volume may reach as many as 0.2 million individuals per year in Hong Kong. The substantial demand for tokay gecko products estimated in this study underscores the urgent need for a thorough assessment of trade sustainability and continuous monitoring of the wild population status of gecko species. In contrast, pangolin scale products were rarely reported, and there was a high level of awareness among sellers regarding the illegality of such products. Our public survey revealed that awareness of the legal status of these products was the most influential factor shaping public choice to support the trade and consequently consume the products. Therefore, setting up legal protection status and enhancing public awareness about the legality of wildlife trade are essential steps toward responsible consumption and conservation. Furthermore, support for the use of alternatives among TCM practitioners presents a promising avenue. This highlights the opportunity to transiting trade toward sustainably sourced alternatives while respecting the traditions associated with TCM.
Sibling and peer bullying victimization in adolescence: Masculinity, femininity, and the moderating role of sex and popularity
Authors: Gaunt Lily , Guy Alexa , Wolke Dieter , Lee Kirsty S. 
Published: January 2024

AbstractIntroductionWe investigated whether gender‐typed traits (masculinity and femininity) contemporaneously predicted self‐reported peer victimization, peer‐reported peer victimization, and sibling victimization. We also tested the moderating role of sex and popularity.MethodsA sample of 2782 British pupils aged 11–16 from Central England, UK was screened for bullying involvement and popularity using self‐report and peer nominations, and a subsample of 704 (52.7% girls) completed a measure of gender‐typed traits (masculinity and femininity).ResultsHierarchical multiple regression analyses revealed that low levels of masculine traits were associated with greater risk of self‐reported peer victimization, there were no associations with peer‐reported peer victimization, and low levels of feminine traits were associated with greater risk of self‐reported sibling victimization. The effects were not moderated by sex, while popularity decreased the risk of self‐ and peer‐reported peer victimization.ConclusionsBullying prevention interventions could benefit from including the positive facets of feminine and masculine traits.
LINC00624 affects hepatocellular carcinoma proliferation and apoptosis through the miR‐342‐3p/DNAJC5 axis
Authors: Xu Huawei , Shen Peng , Fang Jian , Jiang Jihua , Shi Yinsheng , Xu Pengcheng , Jiang Renya , Wang Zhengfei 
Published: January 2024

AbstractLINC00624 is a long noncoding RNA (lncRNA) which was seldom investigated before. The goal of our study is to clarify the expression and underlying network of LINC00624 in hepatocellular carcinoma (HCC). Here, both HCC and normal living cell lines were employed. Real‐time quantitative PCR and western blot were used to determine the pattern of genes and proteins. Colony formation, flow cytometry and western blot tests were used to determine cell proliferation and apoptosis, respectively. Dual luciferase was used to verify molecule‐molecule interactions. LINC00624 expression was increased in HCC cell lines and miR‐342‐3p was decreased. Elimination of LINC00624 increased proliferation while decreasing cell apoptosis. LINC00624 acted as a molecular sponge for miR‐342‐3p, hence facilitating DNAJC5 expression. Functional tests demonstrated that miR‐342‐3p suppression could reverse the effect of LINC00624 silence and overexpression of DNAJC5 significantly mitigated the biological consequences of miR‐342‐3p. These finding demonstrated that LINC00624 aggravated HCC progression by modulating proliferation and apoptosis via targeting miR‐342‐3p/DNAJC5 axis. These data support that inhibition of LINC00624 may a potential treatment strategies of HCC.
Synthesis of novel [1,2,3]triazolo[4′,5′:3,4]pyrrolo[1,2‐a]thieno[2,3‐d] pyrimidines: Potent EGFR targeting anti‐breast cancer agents
Authors: Samala Rajkumar , M Ranadheer Kumar , Bapuram Ashok Kumar , Nasipireddy Venkatarathnam , Narsimha Sirassu 
Published: January 2024

AbstractIn this study, we designed and synthesized several novel fused [1,2,3]triazolo [4′,5′:3,4]pyrrolo[1,2‐a]thieno[2,3‐d]pyrimidine derivatives using in a single [3 + 2] reaction cycloaddition reaction of 3‐(3‐iodoprop‐2‐yn‐1‐yl)thieno[2,3‐d]pyrimidin‐4(3H)‐one (4) followed by C‐C bond coupling with various aryl azides in a PEG‐400 medium. All of the newly synthesized compounds were evaluated in vitro for EGFR kinase inhibitory action as well as anti‐breast cancer activity against MDA‐MB‐231 and MCF‐7 breast cancer cell lines. When compared to the reference molecule, erlotinib, the majority of the compounds demonstrated adequate efficacy. The most promising compounds, 5g and 5i, demonstrated excellent anticancer activity against both cancer cell lines, with IC50 values ranging from 04.17 ± 0.55 to 8.65 ± 0.89 μM, respectively, as well as excellent kinase inhibitory activities (EGFR: IC50 = 0.467 ± 0.063 and 0.412 ± 0.081 μM). The in silico studies of five potent compounds 5f, 5g, 5h, 5i, and 5k were also carried out to identify the interactions against the EGFR receptor and found that the energy calculations were covenant with the obtained IC50 values.
Optimization of an allelic discrimination real‐time RT‐PCR assay for detection of H275Y oseltamivir resistance gene mutation among influenza A(H1N1)pdm09 patients from 2020 to 2022
Authors: Harish Gandhapu , Shetty Ujwal , Varamballi Prasad , Mukhopadhyay Chiranjay , Jagadesh Anitha 
Published: January 2024

AbstractInfluenza virus is known to cause mild to severe respiratory infections and is also prone to genetic mutations. Of all the mutations, neuraminidase (NA) gene mutations are a matter of concern, as most approved antivirals target this protein. During the 2020 influenza season, an emergence of mutation in the NA gene, affecting the binding of the World Health Organization (WHO)‐recommended probes to the specific site of the NA gene, was reported by our group. As a result of this mutation, the WHO‐recommended allelic discrimination real‐time reverse transcriptase polymerase chain reaction (RT‐PCR) assay was unable to detect wild‐type (H275) or mutant oseltamivir‐resistant (Y275) strains of influenza A(H1N1)pmd09 viruses. In the current study, the WHO‐recommended probes were redesigned according to the mutation in the probe binding site. Fifty undetermined samples (2020–2021) from the previous study were retested with the newly designed probes and found to be positive for H275 and/or Y275. The results obtained were similar to the Sanger sequencing results from the previous study, suggesting that the redesigned probes were efficient in discriminating between wild‐type and mutant‐type viruses. Furthermore, 133 samples from 2022, making a total of 183 samples (2020–2022), were tested using improved allelic discrimination real‐time RT‐PCR, and the overall prevalence rate of oseltamivir resistance in 2020–2022 was found to be 0.54%.
The benefits of inclusive organizational behavior: Why diversity climate improves mental health and retention among women during a crisis
Authors: Lacerenza Christina N. , Johnson Stefanie K. , Lambert Brittany , Van Wagoner H. Phoenix 
Published: January 2024

SummaryThe sudden proliferation of virtual work and isolation during the COVID‐19 pandemic has resulted in mental health concerns and increased workplace exits, particularly for women. We examine how inclusive organizational behavior in the form of a positive diversity climate impacts stereotype threat, anxiety, and turnover intentions. Across two studies, we find support for the relationship between diversity climate and stereotype threat for women which relates to anxiety and turnover intentions. Capitalizing on a naturalistic experiment created by COVID‐19, we find women report significant increases in anxiety and turnover intentions after the initial stay‐at‐home orders. We explore the impact to mental health further and find that women working in an organization with a low diversity climate exhibited symptoms that increased to severe levels of anxiety (based on validated clinical cut‐offs) after the initial stay‐at‐home orders. In contrast, women working in a more positive diversity climate did not experience as drastic an increase in anxiety. We interpret these findings through the lens of the job demands–resources model; as a critical job resource, diversity climate reduces gender‐specific job demands for women (i.e., stereotype threat), providing them the ability to cope when they are faced with unforeseen job demands.
Characterization of a Diverse Set of Conditionally Reprogrammed Head and Neck Cancer Cell Cultures
Authors: Ow Thomas J. , Mehta Vikas , Li Daniel , Thomas Carlos , Shrivastava Nitisha , Kawachi Nicole , Gersten Adam J. , Zhu Jing , Schiff Bradley A. , Smith Richard V. , Rosenblatt Gregory , Augustine Stelby , Prystowsky Michael B. , Yin Shanye , Gavathiotis Evripidis , Guha Chandan 
Published: January 2024

ObjectiveTo establish and characterize a diverse library of head and neck squamous cell cancer (HNSCC) cultures using conditional reprogramming (CR).MethodsPatients enrolled on an IRB‐approved protocol to generate tumor cell cultures using CR methods. Tumor and blood samples were collected and clinical information was recorded. Successful CR cultures were validated against banked reference tumors with short tandem repeat genotyping. Cell morphology was archived with photodocumentation. Clinical and demographic factors were evaluated for associations with successful establishment of CR culture. Human papilloma virus (HPV) genotyping, clonogenic survival, MTT assays, spheroid growth, and whole exome sequencing were carried out in selected cultures.ResultsForty four patients were enrolled, with 31 (70%) successful CR cultures, 32% derived from patients who identified as Black and 61% as Hispanic. All major head and neck disease sites were represented, including 15 (48%) oral cavity and 8 (26%) p16‐positive oropharynx cancers. Hispanic ethnicity and first primary tumors (vs. second primary or recurrent tumors) were significantly associated with successful CR culture. HPV expression was conserved in CR cultures, including CR‐024, which carried a novel HPV‐69 serotype. CR cultures were used to test cisplatin responses using MTT assays. Previous work has also demonstrated these models can be used to assess response to radiation and can be engrafted in mouse models. Whole exome sequencing demonstrated that CR cultures preserved tumor mutation burden and driver mutations.ConclusionCR culture is highly successful in propagating HNSCC cells. This study included a high proportion of patients from underrepresented minority groups.Level of EvidenceNot Applicable Laryngoscope, 134:2748–2756, 2024
NT5DC2 knockdown suppresses progression, glycolysis, and neuropathic pain in triple‐negative breast cancer by blocking the EGFR pathway
Authors: Sang Rui , Yu Xiaoping , Xia Han , Qian Xingxing , Yong Jiacheng , Xu Yan , Sun Yan , Yao Yiran , Zhou Jing , Zhuo Shuangshuang 
Published: January 2024

AbstractTriple‐negative breast cancer (TNBC) is an exceptionally aggressive breast cancer subtype associated with neuropathic pain. This study explores the effects of 5′‐nucleotidase domain‐containing protein 2 (NT5DC2) on the progression of TNBC and neuropathic pain. Microarray analysis was conducted to identify differentially expressed genes in TNBC and the pathways involved. Gain‐ and loss‐of‐function assays of NT5DC2 were performed in TNBC cells, followed by detection of the extracellular acidification rate, adenosine triphosphate (ATP) levels, lactic acid production, glucose uptake, proliferation, migration, and invasion in TNBC cells. Macrophages were co‐cultured with TNBC cells to examine the release of polarization‐related factors and cytokines. A xenograft tumor model was established for in vivo validation. In addition, a mouse model of neuropathic pain was established through subepineural injection of TNBC cells, followed by measurement of the sciatic functional index and behavioral analysis to assess neuropathic pain. NT5DC2 was upregulated in TNBC and was positively correlated with epidermal growth factor receptor (EGFR). NT5DC2 interacted with EGFR to promote downstream signal transduction in TNBC cells. NT5DC2 knockdown diminished proliferation, migration, invasion, the extracellular acidification rate, ATP levels, lactic acid production, and glucose uptake in TNBC cells. Co‐culture with NT5DC2‐knockdown TNBC cells alleviated the M2 polarization of macrophages. Furthermore, NT5DC2 knockdown reduced tumor growth and neuropathic pain in mice. Importantly, activation of the EGFR pathway counteracted the effects of NT5DC2 knockdown. NT5DC2 knockdown protected against TNBC progression and neuropathic pain by inactivating the EGFR pathway.
The interplay of information sharing and distribution mode choice under cap‐and‐trade policy in the ecommerce era
Authors: Li Ling , Guo Qiuyu , Jiang Yushan 
Published: January 2024

AbstractThis paper endeavors to examine the interplay between information sharing and distribution mode in the context of low carbon, while considering the cap‐and‐trade. The findings indicate that under the non‐information sharing, the manufacturer tends to opt for the reselling mode when the carbon price is high; under the information sharing, the manufacturer is inclined towards the reselling mode when both the carbon price and investment cost coefficient are high. Interestingly, we find that the information sharing decision of the platform triggers an induced effect on the distribution mode selection of the manufacturer, and this effect is bi‐directional and conditional.