bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated Prenatal and Postnatal Management for Neonates with Transposition of the Great Arteries: Thirteen-Year Experience at A Single Center
Authors: lin xieyi , Huang Ying , Xie Wen , Chen Lu , Huang Yuping , Huang Yu , Ma Bingyu , Wen Shusheng , Pan Wei 
Published: December 2023

Abstract

Background
Transposition of the great arteries (TGA) is the most common cyanotic congenital heart defect in neonates with low prenatal detection rate. This study sought to review the prenatal diagnosis, associated abnormalities, and mid-term postnatal outcomes of fetuses with TGA and investigate the integrated prenatal and postnatal management for TGA neonates.
Methods
Children born with prenatal diagnosis of TGA in Guangdong Provincial People’s Hospital from January 2009 to December 2022 were included in the study. The prenatal ultrasound data and neonatal records were reviewed to assess the accuracy of prenatal diagnosis. Univariate and multivariate logistic and Cox analyses were used to analyze the correlation between risk factors and prognosis of TGA patients.
Results
134 fetuses with prenatal diagnosis of TGA were included in this study and assessed at a median follow-up of 1.57 years [interquartile range (IQR) 0.07–4.28]. The population originated from 40 cities in 10 provinces in China, with integrated antenatal and postnatal management rate reaching 94.0% (126/134) and a high accuracy rate (99.3%) of prenatal primary diagnosis. There were 3 (2.2%) postnatal deaths, 118 (88.1%) patients undergoing Arterial switch operation (ASO), 3 (2.2%) undergoing Restelli operations and 5 (3.7%) doing stage operations. Of 118 patients receiving ASO, the major morbidity occurred in 64 patients, with gestational ages at birth (OR = 0.953, 95% CI 0.910–0.991; P = 0.025) and cardiopulmonary bypass time (OR = 1.010, 95% CI 1.000-1.030; P = 0.038) as independent risk factors. 26.3 percent (n = 31) experienced right ventricular outflow tract obstruction (RVOTO) after surgery. Independent risk factors was aortic cross-clamping time (HR = 1.030, 95% CI 1.000-1.050; P = 0.017).
Conclusion
Premature infants is significantly associated with increased morbidity. Integrated prenatal and postnatal management is recommended for those with prenatal diagnosis of TGA.
Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae s.l mosquitoes after feeding
Authors: Egyirifa Richardson K. , Akorli Jewelna 
Published: December 2023

Abstract
Background
The discovery of high prevalence of Microsporidia MB in both sexes of Anopheles has informed the need to also include male mosquitoes in the study of mosquito-associated microbiome which had mainly been female-focused. In this study, the microbiota of male and female Anopheles gambiae s.l were compared to identify similarity or otherwise of bacterial composition between the two sexes.
Methods
Late larval instars of Anopheles mosquitoes were collected from the field and raised to adults. Equal numbers of males and females of 1-day-old non-sugar-fed, 4–5-day old sugar-fed and post blood-fed females were randomly selected for whole body analyses of bacteria 16S rRNA.
Results
Results reveal that male and female mosquitoes generally share similar microbiota except when females are blood fed. Compared to newly emerged unfed mosquitoes, having sugar and/or blood meal increased dissimilarity in microbial diversity in both males and females with the variation being greater among females (39% P = 0.01) than in males (29% P = 0.03). Elizabethkingia meningoseptica and Asaia siamensis were highly abundant bacteria species (> 0.01 average relative abundance) that were common significant discriminants between groups based on their feeding status. E. meningoseptica was shown to be the only common differentially significant bacteria in male and female mosquitoes that had fed compared to their unfed counterparts (LDA score > 4.0, P < 0.05). Its relative abundance did not change in blood fed females while A. siamensis reduced. In males, however, A. siamensis did not differ significantly post sugar meal.
Conclusions
Our results indicate the opportunities for stable infection in mosquitoes should these promising species be used in bacteria-mediated disease control. Further studies are recommended for clearer understanding of multiple strain tissue tropism for selecting most appropriate and effective transmission blocking strategies.
Predictive Modeling of Land Use and Land Cover Changes Using Q-GIS to Improve Geospatial Decision-Making and Sustainable Strategies
Authors: Dipto Abir Mahmud , Rasel H. M. , Hossain ABM Shafkat , Hossain Iqbal , Saki Saleh Ahmad , Ahad S. M. Abdullah Al 
Published: December 2023

Abstract

A systematic approach to comprehending both physical and non-physical interactions between ecosystems in nature with the aim of ecological sustainability is called LULC alteration analysis. An extensive overview of the development potential for both present and future is provided through an investigation of spatially shifting behaviors of LULC and modeling of prospective eventualities. With a 20-year approximation from 2000 to 2020, we used substantial multi-temporal data collected via satellite to explore opportunities for evolution from one spatiotemporal transition state to a subsequent and prospective LULC model. In the MOLUSCE plugin of Q-GIS, uncorrelated parameters (DEM, gradient, and location concerning roadways) have been combined with an incorporated CA-ANN technique. Throughout previous three decades, impervious surface area increased from 12.48% to 28.91%, while water levels increased from 1.43% to 1.39%, demonstrating that physical and social driving forces had significant influence on landscape pattern. Urbanization and development are reflected from rising water table and shrinking impermeable surface. Land use shifts or climatic variability may result decline in water levels, whereas the rise in impermeable surfaces points in the direction of proliferation of metropolitan region. The quantity of dry land decreased from 48.28% to 43.11%, the verdant area was 27.34% to 13.77%, and woodland shrank from 22.70% to 12.64%. The estimates from 2030 to 2040 further endorse an increasing shift toward impermeable terrain at the expense of substantial forests and natural habitats. For successful land management, urban planning, and sustainable development, LULC trends must be precisely predicted. In-depth reviews and analyses of predictive modeling approaches utilized for LULC prediction are provided in the study. This study investigates frequently used data sources and preprocessing methods, looks at difficulties with LULC prediction, and offers critical assessment of modeling strategies. The report also outlines future research priorities and explores possible uses of the LULC prediction model uses.
Recommendations to improve use and dissemination of patient versions of oncological clinical practice guidelines in Germany: results of a multi-stakeholder workshop
Authors: Könsgen Nadja , Hauprich Julia , Wahlen Sarah , Hellbrecht Irma , Becker Monika , Bühn Stefanie , Meyer Nora , Blödt Susanne , Carl Günther , Follmann Markus , Frenz Stefanie , Langer Thomas , Nothacker Monika , Schaefer Corinna , Pieper Dawid , Breuing Jessica 
Published: December 2023

Abstract

Background:
Oncological patients have high information needs that are often unmet. Patient versions of oncological clinical practice guidelines (PVG) translate clinical practice guidelines into laypersons’ language and might help to address patients’ information needs. Currently, 30 oncological PVG have been published in Germany and more are being developed. Following a large multi-phase project on oncological PVGs in Germany, recommendations to improve use and dissemination of PVG were adopted in a multi-stakeholder workshop.
Methods:
Organisations representing users of PVGs (patients, medical personnel, and multipliers), creators, initiators/funding organisations of PVGs, and organisations with methodological expertise in the development of clinical practice guidelines or in patient health information were invited to participate. The workshop included a World Café for discussion of pre-selected recommendations and structured consensus procedure for of all recommendations. Recommendations with agreement of > 75% were approved, and in case of ≤ 75% agreement, recommendations were rejected.
Results:
The workshop took place on 24th April 2023 in Cologne, Germany. Overall, 23 people from 24 organisations participated in the discussion. Of 35 suggested recommendations 28 recommendations reached consensus and were approved. The recommendations referre to the topics dissemination (N = 13), design and format (N = 7), (digital) links (N = 5), digitalisation (N = 4), up-to-dateness (N = 3), and use of the PVG in collaboration between healthcare providers and patients (N = 3).
Conclusion:
The practical recommendations consider various perspectives and can help to improve use and dissemination of oncological PVG in Germany. The inclusion of different stakeholders could facilitate the transfer of the results into practice.
Bile Acid Metabolism Analysis Insight into the Vascular Endothelial Injury in High Salt-Induced Hypertensive Rats
Authors: Zeng Baihan , Peng Xile , Chen Yixiao , Chen Li , Xia Yanglin , Xia Lina 
Published: December 2023

Abstract
Background: High salt diet has become one of the leading risk factors for cardiovascular diseases worldwide. There is already research to prove that excessive salt intake can cause hypertension and endothelial damage. However, the potential mechanism requires further exploration. In the present study, we aimed to reveal the bile acid metabolism in high-salt-induced vascular endothelial injury in rats.
Methods: A high-salt diet (HSD) induced rats model was established by evaluating the levels of blood pressure and vascular endothelial injury factors. Bile acid metabolism analysis was performed to identify differential bile acids between the normal group and the HSD group. Correlation analysis further detected the relevance between vascular endothelial injury factors and bile acids.
Results: The results reflected that HSD can cause disorders in bile acid metabolism. 10 potential bile acids in plasma were found to be involved in the effects of high salt on vascular endothelium. Further correlation analysis indicated that endothelin-1 (ET-1) has a negative correlation with glycocholic acid, and nitric oxide (NO) has a positive correlation with ursocholic acid.
Conclusion: This study identified which bile acids associated with high-salt-induced vascular endothelial injury, and provided a new idea for the prevention and treatment of high salt-induced cardiovascular diseases.
Molecular epidemiology and multilocus genotyping of Giardia duodenalis in individuals attending major public hospitals in Shiraz, southwestern Iran: A public health concern
Authors: Asghari Ali , Mahdavi Farzad , Shamsi Laya , Mohammadi-Ghalehbin Behnam , Shahabi Saeed , Asgari Qasem , Motazedian Mohammad Hossein , Sadrebazzaz Alireza 
Published: December 2023

Abstract

Giardia duodenalis is one of the most common causes of waterborne disease worldwide, and is often associated with outbreaks of diarrhea in areas with poor sanitation and hygiene. This study aimed to assess the prevalence and genetic diversity of G. duodenalis assemblages in individuals attending major public hospitals in Shiraz, southwestern Iran. From August 2022 to October 2023, a total of 614 stool samples from individuals were collected and initially examined for G. duodenalis cysts using parasitological techniques, sucrose flotation, and microscopy. Microscopy-positive samples were validated by SSU-PCR amplification of the parasite DNA. A multilocus genotyping (MLG) scheme, which focused on the triose phosphate isomerase (tpi) and the glutamate dehydrogenase (gdh) genes, was employed for genotyping purposes. G. duodenalis cysts were found in 7.5% (46/614) and 8.5% (52/614) of samples through microscopy and SSU-PCR, respectively. Successful amplification and sequencing results were obtained for 77.3% (17/22) and 45.5% (10/22) of the infected samples at the tpi and gdh loci, respectively. MLG data for the two loci were available for only five samples. Out of the 22 samples genotyped at any loci, 54.5% (12/22) were identified as assemblage A, while 45.5% (10/22) were identified as assemblage B. AII was the most predominant sub-assemblage identified [54.5% (12/22)], followed by BIII [27% (6/22)], discordant BIII/BIV [13.6% (3/22)], and BIV [4.5% (1/22)]. This study found no assemblages suitable for animal hosts (e.g., C-F), indicating that the transmission of human giardiasis in Shiraz is mainly anthroponotic. Further molecular-based analyses are necessary to confirm and expand upon these findings.
Association between daily activities and cognitive impairment among elderly adults with depression: A nomogram prediction model
Authors: Huang Lifeng , Li Ping 
Published: December 2023

Abstract
Objective: We aim to use the Chinese Longitudinal Healthy Longevity Survey (CLHLS) to develop and validate a targeted model for predicting the risk of cognitive impairment in elderly adults with depression.
Methods: 3658 and 1569 patients from CLHLS were enrolled in the training and validation cohort, respectively. Cognitive function was tested by the Chinese version of the Mini-Mental State Examination (MMSE). Forward stepwise multivariable logistic regression analysis was performed to identify independent risk factors for cognitive impairment in elderly adults with depression, and a visible nomogram was conducted. The evaluation of nomogram’s performance was measured by the area under curve (AUC), calibration curve and decision curve analysis (DCA).
Results: In the final prediction model for cognitive impairment risk, seven essential factors were selected: age, education, frequency of reading newspaper, frequency of watching TV, ability to shop independently, ability to dress independently, and ability to cook. The model performed well, with AUCs of 0.857 and 0.831 in the training and validation cohorts, respectively. Based on the above-mentioned analytical results, a nomogram was created. The calibration curve indicated that the nomogram was well-calibrated. The DCA demonstrated the nomogram's clinical utility.
Conclusion: A model for predicting the risk of cognitive impairment in elderly adults with depression was successfully developed.
Exploring Implementing Questioning the Author in Teaching Reading Texts in EFL Classes
Authors: Alzahrani Mohammed 
Published: December 2023

Abstract
Reading plays an important role in acquiring English for EFL learners. Teaching reading provides opportunities to acquire knowledge of the language, as well as helping learners to interact with the culture and values of the writers. Numerous recent educational reforms in the Saudi society increasingly emphasise the enhancement of learning outcomes in public schools, including the teaching of English. Hence, it is necessary to examine whether the current reading English curriculum in secondary public schools is appropriate for students. One of the perennial problems in this regard is that the reading texts in the English curriculum do not arouse the interest of students. Reading passages in the Saudi English curriculum are predominantly short, unrepresentative of students’ lives and with poor opportunities for critical analysis, which leads to denying learners enjoyment of the literature. Although EFL teachers seem to be enthusiastic about teaching reading and are willing to include enrichment sources in teaching reading, they struggle with the competing demands of completing textbooks on time, as well as poorly resourced libraries in schools. The findings of this study demonstrate that teachers are not able to provide students with hard-copy reading books, or to persuade students to read extra sources, not included in their textbooks. Importantly, English teachers expressed an interest in contributing to enhancing reading contents and teaching strategies for reading in EFL classes. The study findings reveal that the teachers were optimistic about using the QtA in their classrooms, because it contributed to fostering a more positive attitude to reading among their students.
Comprehensive Analysis Revealed P4Hs as New Biomarkers for Prognosis and Immunotherapy in Head and Neck Cancer
Authors: Zhou Huan , Lei Yulin , Luo Jing , Wang Jianmei , Peng Lin , Mou Kelin , Xiang Li , Luo Yuhao 
Published: December 2023

Abstract

Prolyl 4-hydroxylases (P4Hs) are a family of key modifying enzymes in collagen synthesis. Notably, P4Hs have been confirmed to be closely associated with the occurrence and development of tumors. However, the P4Hs expression in Head and Neck Cancer (HNSC), as well as its relationship with prognosis and tumor immunity infiltration has not yet been analyzed. In this article, we investigated the transcriptional expression, survival data and immune infiltration of P4Hs in patients with HNSC from multiple databases. Intriguingly, we found that P4H1-3 expression was significantly higher in head and neck tumor tissues than in normal tissues. Moreover, P4HA1 and P4HA2 were associated with tumor stage, patient prognosis, and immune cell infiltration. P4HA3 was related to patient prognosis, and immune cell infiltration. Correlation experiments have also confirmed that P4HA1 can be a prognosis biomarker and promote the progression of nasopharyngeal carcinoma. These findings suggest that P4HA1-3 may be a novel biomarker for the prognosis and immunotherapy of head and neck cancer, which will be beneficial in providing new therapies for patients with head and neck tumors and thereby improving patient outcomes.
Medicinal chemistry lens on patent data in SureChEMBL
Authors: Gadiya Yojana , Shetty Simran , Hofmann-Apitius Martin , Gribbon Philip , Zaliani Andrea 
Published: December 2023

Abstract
In the pharmaceutical industry, the patent protection of drugs and medicines is accorded particular importance because of the high costs of research and development (R&D), associated with the considerable risks inherent in the creation of novel drugs. Consequently, patents play a pivotal role in ensuring a suitable return on R&D investments, especially those directed toward clinical trials. The patent corpus has been analyzed by researchers to identify freedom-to-operate spaces for novel drug candidates. Several well-established public patent data repositories have enabled automated methodologies for extracting information on therapeutic agents. In this manuscript, we delve into one such publicly available patent database, SureChEMBL, which catalogues patents related to life sciences. Our exploration begins by evaluating the coverage of compounds within SureChEMBL in comparison to other chemical data resources. Additionally, we pinpoint critical sections of patent documents where detailed chemical annotations can be found. Next, we exhibit the compounds' potential to serve as drug candidates by evaluating their conformity to the Rule of Five (Ro5) and its extension, the beyond Ro5 (bRo5). Furthermore, we examine the development stage (preclinical or clinical) reported for these compounds as documented in public databases like ChEMBL. In summation, our investigation aims to provide an estimate of the relevance of the compounds catalogued within the SureChEMBL database in the context of the drug discovery domain.
3D bioprinted CRC model brings to light the replication necessity of an oncolytic vaccinia virus encoding FCU1 gene to exert an efficient anti-tumoral activity
Authors: Marquette Christophe A. , Petiot Emma , Spindler Anita , Ebel Caroline , Nzepa Mael , Moreau Baptiste , Erbs Philippe , Ballou Jean-Marc , Quemeneur Eric , Zaupa Cécile 
Published: December 2023

Abstract
The oncolytic virus represents a promising therapeutic strategy involving the targeted replication of viruses to eliminate cancer cells, while preserving healthy ones. Despite ongoing clinical trials, this approach encounters significant challenges. This study delves into the interaction between an oncolytic virus and the extracellular matrix (ECM). A three-dimensional colorectal cancer model, enriched with ECM through bioprinting, was subjected to infection by an oncolytic virus derived from the vaccinia virus (oVV). The investigation revealed prolonged expression and sustained oVV production. However, the absence of a significant antitumor effect suggested that the virus's progression towards non-infected tumoral clusters was hindered by the ECM. Effective elimination of tumoral cells was achieved by introducing an oVV expressing FCU1 (an enzyme converting the prodrug 5-FC into the chemotherapeutic compound 5-FU) alongside 5-FC. Notably, this efficacy was absent when using a non-replicative vaccinia virus expressing FCU1. Our findings underscore then the crucial role of oVV proliferation in a complex ECM, facilitating payload expression and generating a bystander effect to eradicate tumors. Additionally, this study emphasizes the utility of 3D bioprinting for assessing ECM impact on oVV and demonstrates how enhancing oVV capabilities allows overcoming these barriers. This showcases the potential of 3D bioprinting technology in designing purpose-fit models for such investigations.
Assembly and analysis of the complete mitochondrial genome of a medicinal and economic plant, Santalum album, revealed the existence of a complex branched structure
Authors: Liu Guanghua , Zuo Youwei , Shan Yuanyu , Li Jiaxi , Chen Ying , Gong Xinyi , Liao Xiaomin 
Published: December 2023

Abstract

BackgroundSantalum albumL. is an evergreen tree which is mainly distributes throughout tropical and temperate regions. And it has a great medicinal and economic value. Results In this study, the complete mitochondrial genome (mitochondrial genome) ofS. albumwere assembled and annotated, which could be described by a complex branched structure consisting of three contigs. The lengths of these three contigs are 165,122 bp, 93,430 bp and 92,491 bp. We annotated 34 genes coding for proteins (PCGs), 26 tRNA genes, and 4 rRNA genes. The analysis of repeated elements shows that there are 89 SSRs and 242 pairs of dispersed repeats inS. albummitochondrial genome. Also we found 20 MTPTs among the chloroplast and mitochondria. The 20 MTPTs sequences span a combined length of 22,353 bp, making up 15.52% of the plastome, 6.37% of the mitochondrial genome. Additionally, by using the Deepred-mt tool, we found 628 RNA editing sites in 34 PCGs. Moreover, significant genomic rearrangement is observed betweenS. albumand its associated mitochondrial genomes. Finally, based on mitochondrial genome PCGs, we deduced the phylogenetic ties betweenS. albumand other angiosperms. Conclusions We reported the mitochondrial genome from Santalales for the first time, which provides a crucial genetic resource for our study of the evolution of mitochondrial genome.