bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensitive ESR Determination of Intracellular Oxidative Stress by Using Acyl-Protected Hydroxylamines as New Spin Reagents
Authors: Itoh Osamu , Aoyama Masaaki , Yokoyama Hidekatsu , Obara Heitaro , Ohya Hiroaki , Kamada Hitoshi 
Published: December 2003

Abstract
Several acyl-protected hydroxylamines were synthesized as new spin reagents for ESR measurements of intracellular oxidative stress. These compounds were stable non-radical compounds, but were easily deprotected with esterase to yield hydroxylamines, which were oxidized by oxidants to yield ESR- detectable nitroxide radical. Using an acyl-protected hydroxylamine, a highly sensitive ESR determination procedure was successfully conducted to analyze oxidative stress in human leukocytes.
DNA Cleavage by a Polyethylenimine-appended Porphyrin
Authors: Suenaga Hikaru , Nango Mamoru , Sugiyama Tadatsugu , Iwasaki Toyota , Takeuchi Yoshito , Shinkai Seiji 
Published: December 2003

Abstract
Polyethylenimine (PEI)-appended porphyrin 2 bound DNA more strongly than non-polyethylenimine-appended porphyrins by creation of cationic charges of polyethylenimine and photochemically cleaved a double-stranded DNA.
Paecilospirone, a Unique Spiro[chroman-2,1′(3′H)-isobenzofuran] Derivative Isolated from Tropical Marine Fungus Paecilomyces sp.
Authors: Namikoshi Michio , Kobayashi Hisayoshi , Yoshimoto Takeshi , Meguro Shiori 
Published: December 2003

Abstract
A spiroacetal compound named paecilospirone was isolated from a marine fungus Paecilomyces sp. Collected at Yap Island. Paecilospirone was found by the screening assay for detecting inhibitors of microtubule assembly using conidia of Pyricularia oryzae. The structure was assigned based on the spectroscopic data. This is the first report of the isolation and characterization of spiro[chroman-2,1′(3′H)-isobenzofuran] derivative as a natural product.
Enormous Size Growth of Thiol-passivated Gold Nanoparticles Induced by Near-IR Laser Light
Authors: Niidome Yasuro , Hori Ayako , Sato Takuro , Yamada Sunao 
Published: December 2003

Abstract
Irradiation of pulsed 1064-nm laser light to dodecanethiol-passivated gold nanoparticles in cyclohexane resulted in enormous growth of the particles. The size increased up to ∼200 nanometers.
Sensitization of Europium(III) Luminescence by DTPA Derivatives
Authors: Ozaki Hiroaki , Suda Emiko , Nagano Toshihisa , Sawai Hiroaki 
Published: December 2003

Abstract
Novel ligands, diethylenetriaminepentaacetic acid bearing several aromatic amines, were synthesized and their chelates with a europium ion were prepared. The lanthanide luminescence of the chelates of 1-aminonaphthalene and 7-amino-4-methylcoumarin was largely enhanced by the energy transfer from the ligand to the europium ion.
Photooxidation of Methane to Methanol by Molecular Oxygen On Water-preadsorbed Porous TiO2-based Catalysts
Authors: Chen Xihui , Li Shuben 
Published: December 2003

Abstract
At the temperature below 350 K, methanol was formed from the photooxidation of CH4 by molecular oxygen on water preadsorbed porous TiO2-based catalysts, Mo-containing porous TiO2 catalysts exhibit higher catalytic activity than pure TiO2.
Highly Efficient Photon-to-Electron Conversion of Mercurochrome-sensitized Nanoporous ZnO Solar Cells
Authors: Hara Kohjiro , Horiguchi Takaro , Kinoshita Tohru , Sayama Kazuhiro , Sugihara Hideki , Arakawa Hironori 
Published: December 2003

Abstract
Mercurochrome showed efficient performance as an organic dye photosensitizer for a solar cell based on nanoporous ZnO thin film electrodes. A 2.5% solar energy conversion efficiency was accomplished under AM 1.5 (99 mW cm−2) with a short-circuit photocurrent density (Jsc) of 7.44 mA cm−2, an open-circuit photovoltage (Voc) of 0.52 V, and a fill factor of 0.64. A monochromatic incident photon-to-current efficiency (IPCE) reached 69% at 510 nm.
Enzyme-catalyzed Ring-opening Polymerization of β-Butyrolactone Using PHB Depolymerase
Authors: Suzuki Yoichi , Ohura Takeshi , Kasuya Ken-ichi , Toshima Kazunobu , Doi Yoshiharu , Matsumura Shuichi 
Published: December 2003

Abstract
Poly(3-hydroxybutyrate) [P(3HB)] was prepared by the ring-opening polymerization of (R,S)-β-butyrolactone (BL) using two types of PHB depolymerase with or without substrate-binding domains (SBD) as the catalyst. The SBD lacking PHB depolymerase exhibited better catalytic activities for the ring-opening polymerization of BL.
Morphological Behavior for Micelle from Polystyrene-b-poly[2-(β-d-glucopyranosyloxy)ethyl acrylate] upon Changing the Copolymer Concentration
Authors: Liang Yu-Zeng , Li Zi-Chen , Li Fu-Mian 
Published: December 2003

Abstract
Multiple morphologies of molecular assemblies of polystyrene-b-poly[2-(β-d-glucopyranosyloxy)ethyl acrylate] (PSt-b-PGEA) were obtained by initially dissolving it in DMF and then adding water to the organic solution. With increasing the initial copolymer concentration (ICC) from 0.1 wt% to 2.0 wt% in DMF, the molecular assemblies of PSt77-b-PGEA6 were transformed from spheres, to rods, to vesicles, to tubules, and again to vesicles. Concanavalin (Con A) was used to characterize the surface properties of the resulting aggregates.
Analysis of Two Stages Dehydrochlorination of Poly(vinyl chloride) Using TG-MS
Authors: Yoshioka Toshiaki , Akama Tetsuhiro , Uchida Miho , Okuwaki Akitsugu 
Published: December 2003

Abstract
Degradation behavior of poly(vinyl chloride) (PVC) was analyzed by means of simultaneous thermogravimetry-mass spectrometry (TG-MS). Weight loss and the production behavior of hydrogen chloride and organic materials with PVC degradation was clearly divided into three stages at lower heating rate. Dehydrochlorination was occurred mainly in the first and second stages. Benzene was formed mainly in the first stage with dehydrochlorination.
Conformational Dynamics of Alkyl Chains in the Lamellar Phase of a Nonionic Surfactant C12E3 Studied by Deuterium Nuclear Magnetic Resonance
Authors: Tonegawa Akimitsu , Ohno Keiichi , Matsuura Hiroatsu , Yamada Koji , Okuda Tsutomu 
Published: December 2003

Abstract
Deuterium NMR spectroscopy was applied to a study of conformational dynamics of alkyl chains in the lamellar phase of a C12E3 (nonionic surfactant)–water system. The order parameter derived has shown that addition of the surfactant in the lamellar phase causes conformational disordering of alkyl chains, leading to a transition to an isotropic solution phase, while addition of water, which eventually leads to phase separation, causes no substantial disordering of alkyl chains.
Lauryl and Stearyl Thioglycosides: Preparation and Reactivity of the Glycosyl Donor
Authors: Matsui Hideaki , Furukawa Jun-ichi , Awano Takuro , Nishi Norio , Sakairi Nobuo 
Published: December 2003

Abstract
Lauryl and stearyl thioglycosides were prepared by the Lewis acid catalyzed reaction of 1-O-acetylated sugars with non-volatile and almost odorless 1-dodecanethiol and 1-octadecanethiol, respectively. These thioglycosides were activated by N-iodosuccinimide-TfOH to glycosyl donors producing disaccharides in good yields.
Unexpected Formation of Novel [4.3.3]Propellane-type Trilactone by Dehydration of Aliphatic Tetracarboxylic Acid
Authors: Kato Jun , Kudo Kazuaki , Shiraishi Shinsaku 
Published: December 2003

Abstract
Dehydration of 3-carboxy-3-(carboxymethyl)hexanedioic acid with excess acetic anhydride resulted in the formation of a novel propellane-type trilactone (orthoester), 2,9,10-trioxa[4.3.3]propellane-3,8,11-trione. This is the first one-step synthesis of a propellane from an acyclic compound.
A Universal Topology in Nonlinear Electrochemical Systems
Authors: Li Zelin , Yu Yan , Liao Hua , Yao Shouzhuo 
Published: December 2003

Abstract
In this paper we present an interesting universal topology occurring in different kinds of electrochemical oscillatory systems, namely, a crossing cycle in the cyclic voltammograms, which means there are a pair of overlapping negative and positive nonlinear feedbacks. The topology can provide with a simple intuition prediction as to whether or not an electrochemical system is possible to oscillate with an appropriate choice of parameters.
Surface Properties of Polysilane Copolymers with Siloxane Pendant Groups
Authors: Matsukawa Kimihiro , Ishikawa Masami , Inoue Hiroshi 
Published: December 2003

Abstract
Polysilane copolymers with disiloxane pendant groups were prepared from methylphenyldichlorosilane and pentamethyldisiloxypropylphenyldichlorosilane as a comonomer by Wurtz coupling. Disiloxane pendant groups were accumulated at the surface of copolysilane films, which was evidenced by XPS analysis. Photoconductivity of these copolysilanes by Xerographic discharge measurement was not much affected by the disiloxane groups.
Improved Synthesis and Crystal Structure of TiCl3(tmeda)(thf): A Highly Stereoselective Pinacol Coupling Reagent for Aromatic Aldehydes
Authors: Oshiki Toshiyuki , Kiriyama Takayuki , Tsuchida Kazuaki , Takai Kazuhiko 
Published: December 2003

Abstract
A monomeric titanium(III) complex, TiCl3(tmeda)(thf) (1), has been prepared by a reaction of TiCl4 with TMEDA, Zn, and a catalytic amount of PbCl2 in an almost quantitative yield. The solid state structure of 1 is revealed by X-ray crystallographic analysis. The complex 1 couples aromatic aldehydes under mild conditions to give 1, 2-diols in good to excellent yields with high dl-selectivities.
Efficient Diastereoselective Pinacol Reaction of Aliphatic and Aromatic Aldehydes by Combined Use of Newly Utilized Titanium(II) Bromide and Copper
Authors: Mukaiyama Teruaki , Kagayama Akifumi , Igarashi Koji 
Published: December 2003

Abstract
Newly utilized low-valent titanium reductant, titanium(II) bromide, was conveniently prepared by treating titanium(IV) bromide with hexamethyldisilane. The pinacol reaction of aromatic and aliphatic aldehydes including primary aldehydes proceeded smoothly in dichloromethane-pivalonitrile at temperatures ranging from −23 to 0 °C by the combined use of soluble titanium(II) bromide and copper to give 1,2-diols in good to high yields with good to high dl-selectivities.
Photochemical Transformation of γ,δ-Unsaturated Ketone O-(p-Cyanophenyl)oximes to 3,4-Dihydro-2H-pyrrole Derivatives
Authors: Mikami Tetsuhiro , Narasaka Koichi 
Published: December 2003

Abstract
γ,δ-Unsaturated ketone O-(p-cyanophenyl)oximes are transformed to 3,4-dihydro-2H-pyrroles by the photosensitized electron transfer process.
Electrochemistry of Fullerene C60 Embedded in a Gel-like Membrane of Tetraoctylphosphonium Bromide on an Electrode in Aqueous Solution
Authors: Nakanishi Takashi , Murakami Hiroto , Sagara Takamasa , Nakashima Naotoshi 
Published: December 2003

Abstract
In this communication, we describe the first finding that fullerene C60 embedded in a gel-like membrane formed from tetraoctylphosphonium bromide on an electrode in an aqueous system gives stable electrochemistry leading to the formation of C60−, C602− and C603−.
Direct Observation of Conformers Caused by the Difference in Ligand Orientation in Low Spin Iron(III) Porphyrin Complex
Authors: Ikeue Takahisa , Yamaguchi Tatsuya , Ohgo Yoshiki , Nakamura Mikio 
Published: December 2003

Abstract
EPR spectrum of low spin bis(dimethylaminopyridine)(3,8,13,18-tetramesityl-2,7,12,17-tetramethylporphyrinato)iron(III) perchlorate taken in frozen CH2Cl2 solution at 4.2 K showed both large gmax and rhombic type signals. The result has been explained in terms of the presence of two conformers where the dihedral angles between two ligands are different.
TEM Observation of Interstratified Ni-Serpentine/Smectite Compounds
Authors: Nagase Takako , Ebina Takeo , Torii Kazuo , Iwasaki Takashi , Hayashi Hiromichi , Onodera Yoshio , Chatterjee Maya 
Published: December 2003

Abstract
Interstratified Ni-serpentine/smectite = 2/1 compound was described by transmission electron microscopy (TEM) for the first time. The image of the lattice fringe coincided with the result of simulation, and it is clearly different from that of chlorite/smectite = 1/1 mineral which has a similar periodicity in X-ray diffraction pattern. The image of domains curving to the direction of their serpentine-part, suggested that the surface of the particle was mainly consisting of Si-O sheets.
High Spin Molecules: [Mn12O12(O2CCH2Cl)16(H2O)4] and the One-electron Reduction Product [PPh4][Mn12O12(O2CCH2Cl)16(H2O)3]
Authors: Tsai Hui-Lien , Jwo Tyn-Yih , Lee Gene-Hsiang , Wang Yu 
Published: December 2003

Abstract
The complex of [Mn12O12(O2CCH2Cl)16(H2O)4] was reduced by PPh4I to give a reduced complex [PPh4][Mn12O12(O2CCH2Cl)16(H2O)3]. Crystal structures of both complexes were determined by the X-ray method. The dc magnetic susceptibility measurements indicate that the neutral and reduced complexes are high spin species.
Photochromism of Titanium Oxide Gels Prepared by the Salt-Catalytic Sol-Gel Process
Authors: Matsumoto Taki , Murakami Yasushi , Takasu Yoshio 
Published: December 2003

Abstract
Photochromism for the monolithic gel of titanium oxide prepared by the catalytic sol-gel process was observed. When hydrazine monohydrochloride was used as a catalyst, an intense blue color development was observed with the formation of Ti3+ on the irradiation of UV light (&lt; ca. 350 nm). The degree of coloration and the recovery time were strongly affected by a kind of salt catalysts although the color was independent. Presence of active salt catalysts in the gel was indispensable for color development.
Encapsulation of Two Types of Chloroform Dimers in the Cavities of a Coordination Polymer
Authors: Biradha Kumar , Fujita Makoto 
Published: December 2003

Abstract
CHCl3 molecules were found to form two types of dimers via C-H···Cl and C-Cl···Cl-C interactions in the cavities of a coordination polymer. The Cambridge Structural Database studies indicated that the Cl···Cl dimer is the first example of CHCl3 with Cl to Cl distance (3.478(4), 3.501(3) Å) below the sum of the van der Waals radii of two Cl-atoms.
HPLC Enantioseparation with Cellulose Tris(3,5-dichlorophenylcarbamate) in Aqueous Methanol as a Mobile Phase
Authors: Chankvetadze Bezhan , Yamamoto Chiyo , Okamoto Yoshio 
Published: December 2003

Abstract
The appropriate design of mobile and stationary phase combinations allowed the use of cellulose tris(3,5-dichlorophenylcarbamate) (CDCPC) as the chiral stationary phase (CSP) in high-performance liquid chromatography (HPLC). Together with previous data obtained in n-hexane/2-propanol as a mobile phase the present study indicates very high chiral resolving ability of CDCPC.
Methylene Blue Sensitized Degradation of Sodium Hyaluronate through Photoinduced Electron Transfer
Authors: Kojima Masanobu , Takagi Tomoko , Matsubara Chie , Nakamura Kimitoshi 
Published: December 2003

Abstract
Sodium hyaluonate (NaHA) and Methylene Blue (MB) formed a complex through charge transfer (CT) interaction or ion association in aqueous solution. Irradiation of the complex using a 313-nm light under argon caused degradation of NaHA. The mechanism proposed on the basis of the effect of excitation wavelength and oxygen, and by comparison with the degradation of NaHA using Rose Bengal (RB) and TiO2, was that photoinduced electron transfer from NaHA to upper excited state MB initiated the degradation.
Hydrolysis of Oligonucleotides by Homogeneous Ce(IV)/EDTA Complex
Authors: Igawa Tomoyuki , Sumaoka Jun , Komiyama Makoto 
Published: December 2003

Abstract
Homogeneous Ce(IV) complex of EDTA promptly hydrolyzes oligonucleotides under physiological conditions. In contrast, dinucleotides are not hydrolyzed to measurable extents. The degree of polymerization of DNA substrate is crucial for the present catalysis.
A Simple Route to Selective Diels-Alder Reactions Using Modified Zeolite Y
Authors: Najafi H Mahmoudi , Ghandi M , Farzaneh F 
Published: December 2003

Abstract
Synthetically useful Diels-Alder reactions of different dienophiles with cyclopentadiene in the presence of chirally modified zeolite Y as simple and accessible medium led to high rate and diastereoselectivity and low to moderate enantioselectivity.
Color and Fluorescence Imaging with t-Boc-protected Dihydroxynaphthacenedione
Authors: Kim Jong-Man , Kim Jun-Young , Kim Tae-Ho , Han Dong Keun , Ahn Kwang-Duk 
Published: December 2003

Abstract
In recent years, generation of patterned images in polymer films has become a central focus in a variety fields of chemistry. Recently, generation of functional images by selective immobilization of organic dyes in the polymer film has been reported.1-3 Selective removal of acid labile protecting groups through photoinduced chemical transformation followed by chemisorption of organic dyes from solution into the patterned polymer film afforded µm-scale functional images.
Generation, Structure, and Reactivity of o-Iodobenzoyloxyl Radicals. Pulsed Laser Photolysis of 1-(o-Halobenzoyloxy)-2-pyridones
Authors: Hashimoto Ji-ichiro , Segawa Katsunori , Itoh Hiroki , Sakuragi Hirochika 
Published: December 2003

Abstract
The transient absorption spectrum of o-iodobenzoyloxyl radicals was observed for the first time in acetonitrile using 1-(o-iodobenzoyloxy)-2-pyridone as a precursor. On the basis of the comparison of the spectra of o-halobenzoyloxyl radicals, it is proposed that the o-iodobenzoyloxyl radicals take a planar structure with a strong interaction between the radical center oxygen atom and the neighboring iodine atom.
Photoinduced Deracemization of [Co(acac)3] (acac− = acetylacetonate) with Chiral Δ-[Ru(menbpy)3]2+ (menbpy = 4,4′-bis{(1R,2S,5R)-(−)-menthoxycarbonyl}-2,2′-bipyridine)
Authors: Hamada Taisuke , Ohtsuka Hiroshi , Sakaki Shigeyoshi 
Published: December 2003

Abstract
Deracemization of a racemic mixture of Δ- and Λ-[Co(acac)3] (acac− = acetylacetonate) was successfully carried out with a chiral ruthenium(II) complex, Δ-[Ru(menbpy)3]2+ [menbpy = 4,4′-bis{(1R,2S,5R)-(−)-menthoxycarbonyl}-2,2′-bipyridine], in acetnitrile-H2O (7:3 v/v), in the presence of either triethylamine or NaOH under visible light irradiation (420 &lt; λ &lt; 470 nm). The Λ-isomer was obtained preferentially with the enantiomeric excess of 38%.
The Juliá-Colonna Type Asymmetric Epoxidation Reaction Catalyzed by Soluble Oligo-l-leucines Containing an α-Aminoisobutyric Acid Residue: Importance of Helical Structure of the Catalyst on Asymmetric Induction
Authors: Takagi Ryukichi , Shiraki Akiko , Manabe Toshiki , Kojima Satoshi , Ohkata Katsuo 
Published: December 2003

Abstract
Length-defined organic solvent soluble oligo-l-leucines containing an Aib residue were prepared by stepwise elongation and fragment condensation methods, and were used as catalysts in the Juliá–Colonna asymmetric epoxidation reaction. The yield and enantioselectivity rose by increasing the number of amino acid units in the catalyst. The enantioselectivity was very sensitive to the reaction solvent. The IR characteristic bands (the amide I region) in CH2Cl2 indicated the soluble catalysts to be of helical structure in solution.
Highly Efficient Generation of Ammonium Eneselenolates, Their Reactions and Electronic Properties
Authors: Murai Toshiaki , Hayakawa Shuuya , Kato Shinzi 
Published: December 2003

Abstract
The ammonium eneselenolates generated from selenothioic acid S-esters and ammonium fluoride were reacted with carbon electrophiles to furnish ketene selenothioacetals with high stereoselectivities. The spectroscopic properties of the ammonium eneselenolates have suggested that the electrons on the selenium atom efficiently delocalize on the carbon-carbon double bond.
Properly Assembled Dendrons Can Be Immobilized into Dendrimers by in situ Cross-link
Authors: Numata Munenori , Ikeda Atsushi , Shinkai Seiji 
Published: December 2003

Abstract
Aggregates obtained from saccharide-containing dendrons were immobilized by in situ cross-link with 1,3-phenylene diisocyanate. Characterizations of the resultant oligomers by dynamic light scattering and MALDI-TOF Mass have established that the higher generation dendrons which tend to aggregate into a spherical structure result in a dendritic polymer whereas the lower generation dendrons which tend to aggregate into a disordered large particle cannot result in such a dendrimer-shaped oligomer.
Transesterification vs Hydrolysis: the Reactivity of Metal-Bound Hydroxo Moiety in [TPANi(II)(μ-OH)2Ni(II)TPA](ClO4)2 (TPA = tris(2-pyridylmethyl)amine)
Authors: Ito Masami , Kawano Hiroyuki , Takeuchi Takashi , Takita Yu-saku 
Published: December 2003

Abstract
In the reaction of triphenyl phosphate with ethanol in the presence of complex 1, the transesterification to give diethyl phenyl phosphate was observed, in which system direct nucleophilic attack of ligated hydroxo moiety in 1 toward triphenyl phosphate also proceeded to give µ-phosphate complex, 2.
A Photocrosslinkable Dendrimer Consisting of a Nucleobase
Authors: Tominaga Masahide , Konishi Katsuaki , Aida Takuzo 
Published: December 2003

Abstract
Upon irradiation at 280 nm in CH3CN, dendritic polyuracils LnU-OBn (n = 3, 4) were crosslinked by a [2+2] photodimerization of the uracil units to give intramolecularly “locked” dendrimers, which were unlocked by irradiation at 240 nm.
Photocatalytic Reduction of Acetylpyridine to Pinacol Using [fac-Re(bpy)(CO)3{4-(MeCO)py}]+ (bpy = 2,2′-bipyridine, py = pyridine)
Authors: Hori Hisao , Koike Kazuhide , Takeuchi Koji , Ishitani Osamu 
Published: December 2003

Abstract
[fac-Re(bpy)(CO)3{4-(MeCO)py}]+ can act as an efficient photocatalyst for the reduction of 4-acetylpyridine to a pinacol (py-CMeOHCMeOH-py) in the presence of triethanolamine. The reaction proceeds via formation of a pinacol complex [fac-Re(bpy)(CO)3(py-CMeOHCMeOH-py)]+.
Three-Dimensional Ordered Macroporous Structures With Mesoporous Silica Walls
Authors: Luo Qian , Li Li , Yang Bai , Zhao Dongyuan 
Published: December 2003

Abstract
Three-dimensional (3D) cubic ordered macroporous (140 nm) and binary macroporous (140, 80 nm) silica structures with ordered mesoporous (7.7 nm) walls have been synthesized by using cubic closed-packing colloidal crystals as templates. The colloidal crystals can be made from equal-sized or binary-sized latex spheres by the simple methods of self-sedimentation on the film or pressing into a pellet.
Decomposition of N-Nitrosamines over Zeolites
Authors: Shen Bin , Ma Li Li , Zhu Jian Hua , Xu Qin-hua 
Published: December 2003

Abstract
The strong adsorption of zeolite Y and ZSM-5 for N-nitrosamine such as N-nitrosodimethylamine and N-nitrosopyrrolidine was firstly revealed by use of adsorption, temperature programmed surface reaction and TG-DTA techniques. Zeolite can adsorb N-nitrosamines without interference of organic solvent, which will be helpful for removal of the N-nitrosamines pollution in environmental protection.
Low Temperature Synthesis of Titania Gel Containing Anatase and Rutile
Authors: Uekawa Naofumi , Kajiwara Jyunichi , Miyatake Kazuki , Kakegawa Kazuyuki , Sasaki Yoshinori 
Published: December 2003

Abstract
A crystallized titania gel was prepared without gel-collapse and precipitation by heat treatment at 348K for 24h of an aqueous ethanol solution of the Ti-peroxy compound obtained from Ti(OiPr)4 and H2O2. The structure of the TiO2 in the gel changed from rutile to anatase as the ethanol concentration in the gel increased.
Facile Preparation of a Fluorescence-labeled Plasmid
Authors: Ishii Tsuyoshi , Okahata Yoshio , Sato Toshinori 
Published: December 2003

Abstract
We developed a facile method for labeling plasmid DNA with a fluorescent probe (FITC). The obtained FITC-labeled plasmid kept luciferase activity, and had enough fluorescence intensity to monitor with flow cytometer and confocal laser scanning microscope.