bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia
Authors: Bisaccia Paola , Magarotto Fabio , D’Agostino Stefania , Dedja Arben , Barbon Silvia , Guidolin Diego , Liboni Cristina , Angioni Roberta , De Lazzari Giada , Caicci Federico , Viola Antonella , Jurga Marcin , Kundrotas Gabrielis , Stevens Dimitri , Mancuso Domenico , Gramegna Elisabetta , Seitaj Bruno , Kashyap Rudra , De Vos Beatrice , Macchi Veronica , Baraldi Eugenio , Porzionato Andrea , De Caro Raffaele , Muraca Maurizio , Pozzobon Michela 
Published: November 2023

Abstract
Oxidative stress and fibrosis are important stress responses that characterize bronchopulmonary dysplasia (BPD), a disease for which only a therapy but not a cure has been developed. In this work, we investigated the effects of mesenchymal stromal cells-derived extracellular vesicles (MSC-EVs) on lung and brain compartment in an animal model of hyperoxia-induced BPD. Rat pups were intratracheally injected with MSC-EVs produced by human umbilical cord-derived MSC, following the Good Manufacturing Practice-grade (GMP-grade). After evaluating biodistribution of labelled MSC-EVs in rat pups left in normoxia and hyperoxia, oxidative stress and fibrosis investigation were performed. Oxidative stress protection by MSC-EVs treatment was proved both in lung and in brain. The lung epithelial compartment ameliorated glycosaminoglycan and surfactant protein expression in MSC-EVs-injected rat pups compared to untreated animals. Pups under hyperoxia exhibited a fibrotic phenotype in lungs shown by increased collagen deposition and also expression of profibrotic genes. Both parameters were reduced by treatment with MSC-EVs. We established an in vitro model of fibrosis and another of oxidative stress, and we proved that MSC-EVs suppressed the induction of αSMA, influencing collagen deposition and protecting from the oxidative stress. In conclusion, intratracheal administration of clinical-grade MSC-EVs protect from oxidative stress, improves pulmonary epithelial function, and counteracts the development of fibrosis. In the future, MSC-EVs could represent a new cure to prevent the development of BPD.
Procoagulant Properties of Mesenchymal Stem Cells and Extracellular Vesicles: A Novel Aspect of Thrombosis Pathogenesis
Authors: Yang Bianlei , Long Yaoying , Zhang Anyuan , Wang Hongxiang , Chen Zhichao , Li Qiubai 
Published: November 2023

Abstract
Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types and secrete extracellular vesicles (EVs) that transport bioactive molecules and mediate intercellular communication. MSCs and MSC-derived EVs (MSC-EVs) have shown promising therapeutic effects in several diseases. However, their procoagulant activity and thrombogenic risk may limit their clinical safety. In this review, we summarize current knowledge on procoagulant molecules expressed on the surface of MSCs and MSC-EVs, such as tissue factor and phosphatidylserine. Moreover, we discuss how these molecules interact with the coagulation system and contribute to thrombus formation through different mechanisms. Additionally, various confounding factors, such as cell dose, tissue source, passage number, and culture conditions of MSCs and subpopulations of MSC-EVs, affect the expression of procoagulant molecules and procoagulant activity of MSCs and MSC-EVs. Therefore, herein, we summarize several strategies to reduce the surface procoagulant activity of MSCs and MSC-EVs, thereby aiming to improve their safety profile for clinical use.
A Genome Sequence Resource of Diaporthe nobilis Causing Kiwifruit Rot
Authors: Li Huanhuan , Yu Shukun , Tang Xiaofeng , Liu Yongsheng , Miao Min 
Published: November 2023

Diaporthe spp. are a significant plant pathogen causing wood canker, blight, dieback, and fruit decay in numerous economically important plant hosts worldwide. D. nobilis has been identified as a highly pathogenic isolate responsible for causing kiwifruit ( Actinidia spp.) rot, representing one of the most significant threats to the kiwifruit industry. Here, a comprehensive genome-wide sequence of D. nobilis strain DJY16A 5-1 utilizing advanced Nanopore third-generation sequencing technology is presented. The genome of D. nobilis strain DJY16A 5-1 was assembled into 26 contigs containing a combined size of 59.28 Mb and N50 length of 4.47 Mb. The virulence-related genes were analyzed, including 3,407 transmembrane proteins, 1,410 secreted proteins, 1,866 signal peptides, 135 effector proteins, 1,069 carbohydrate-active enzymes, 143 membrane transporter proteins, and 4,995 pathogen–host interaction-related proteins. The genome assembly and annotation facilitate the analysis of pathogen–host interaction, laying the foundation for in-depth research on the infection mechanism of pathogens. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Associations between weekly gestational exposure of fine particulate matter, ozone, and nitrogen dioxide and preterm birth in a North Carolina Birth Cohort, 2003–2015
Authors: Krajewski Alison K. , Luben Thomas J. , Warren Joshua L. , Rappazzo Kristen M. 
Published: November 2023

Background:
Preterm birth (PTB; <37 weeks completed gestation) is associated with exposure to air pollution, though variability in association magnitude and direction across exposure windows exists. We evaluated associations between weekly gestational exposure to fine particulate matter (PM2.5), nitrogen dioxide (NO2), and ozone (O3) with PTB in a North Carolina Birth Cohort from 2003 to 2015 (N = 1,367,517).

Methods:
Daily average PM2.5 and daily 8-hour maximum NO2 concentration estimates were obtained from a hybrid ensemble model with a spatial resolution of 1 km2. Daily 8-hour maximum census tract-level concentration estimates for O3 were obtained from the EPA’s Fused Air Quality Surface Using Downscaling model. Air pollutant concentrations were linked by census tract to residential address at delivery and averaged across each week of pregnancy. Modified Poisson regression models with robust errors were used to estimate risk differences (RD [95% confidence intervals (CI)]) for an interquartile range increase in pollutants per 10,000 births, adjusted for potential confounders.

Results:
Associations were similar in magnitude across weeks. We observed positive associations for PM2.5 and O3 exposures, but generally null associations with NO2. RDs ranged from 15 (95% CI = 11, 18) to 32 (27, 37) per 10,000 births for PM2.5; from −7 (−14, −1) to 0 (−5, 4) for NO2; and from 4 (1, 7) to 13 (10, 16) for O3.

Conclusion:
Our results show that increased PM2.5 exposure is associated with an increased risk of PTB across gestational weeks, and these associations persist in multipollutant models with NO2 and/or O3.
Keratoconus Management in the Community: A Survey of Optometrists
Authors: Angelo Lize , Gokul Akilesh , McGhee Charles N.J. , Ziaei Mohammed 
Published: November 2023

Objectives:
Defining the patterns of practice and referral criteria of optometrists within New Zealand to investigate the diagnosis and management of keratoconus.

Methods:
Optometrists recruited through the New Zealand Association of Optometrists, Cornea and Contact Lens Society of New Zealand, and private practices were invited to complete an anonymous survey.

Results:
Responses were received from 168 optometrists (representing 20.0% of the optometrist population). Half (48%) of optometrists had ≥15 years of experience, and 22% prescribed soft contact lenses daily, whereas only 6.4% prescribed rigid gas-permeable (RGP) lenses daily. The main barriers to prescribing RGPs were experience with fitting, low market demand, and patient discomfort. When referring to an ophthalmologist, 41% reported referring on progression of corneal parameters, 27% on initial diagnosis, 21% at no set time, and 10% with a reduction in visual acuity. Most optometrists (64%) would refer for possible surgery when visual acuity dropped between 6/9 and 6/12. Optometrists with greater experience were more likely to prescribe RGP lenses and co-manage patients with ophthalmologists. Ownership of a corneal imaging unit suggested an increased likelihood of prescribing RGP lenses but did not alter referral patterns.

Conclusion:
This survey provides an overview of current practice and highlights the importance of optometrists in the diagnosis and management of keratoconus. There was a significant discrepancy in keratoconus management, regarding optical correction modality and referral criteria for ophthalmology review. Further interdisciplinary work is required between optometry and ophthalmology to standardize referral guidelines and enhance visual outcomes.
Accuracy of an Air-Puff Dynamic Tonometry Biomarker to Discriminate the Corneal Biomechanical Response in Patients With Keratoconus
Authors: Lombardo Giuseppe , Alunni-Fegatelli Danilo , Serrao Sebastiano , Mencucci Rita , Roszkowska Anna Maria , Bernava Giuseppe Massimo , Vestri Annarita , Aleo Danilo , Lombardo Marco 
Published: November 2023

Purpose:
The aim of this study was to assess accuracy of the mean corneal stiffness (k

c
, N/m) parameter to discriminate between patients with keratoconus and age-matched healthy subjects.

Methods:
Dynamic Scheimpflug imaging tonometry was performed with Corvis ST (Oculus Optikgeräte GmbH, Germany) in patients with keratoconus (n = 24; study group) and age-matched healthy subjects (n = 32; control). An image processing algorithm was developed to analyze the video sequence of the Corvis ST air-puff event and to determine the geometric and temporal parameters that correlated with the corneal tissue biomechanical properties. A modified 3-element viscoelastic model was used to derive the k

c
parameter, which represented the corneal tissue resistance to deformation under load. Receiver operating characteristic curves were used to assess the overall diagnostic performance for determining the area under the curve, sensitivity, and specificity of the k

c
in assessing the corneal tissue deformation to the Corvis ST air-puff event in keratoconus and control eyes. The Corvis Biomechanical Index (CBI) was analyzed for external validation.

Results:
The k

c
parameter was significantly different between keratoconus and controls (P < 0.001), ranging from 24.9 ±3.0 to 34.2 ±3.5 N/m, respectively. It was highly correlated with CBI (r = −0.69; P < 0.001); however, the k

c
parameter had greater specificity (94%) than CBI (75%), whereas the 2 biomarkers had similar area under the curve (0.98 vs. 0.94) and sensitivity (96% vs. 92%) in predicting the occurrence of keratoconus.

Conclusions:
The k

c
parameter extracted by video processing analysis of dynamic Scheimpflug tonometry data was highly accurate in discriminating patients with clinically manifest keratoconus compared with controls.
Home Care Workers’ Objective and Subjective Recovery From Work
Authors: Säynäjäkangas Pihla , Mänttäri Satu , Selander Kirsikka , Laitinen Jaana 
Published: November 2023

Objective
The aim of this cross-sectional study was to examine the recovery from work in Finnish home care nurses with objective and subjective recovery measures.

Methods
Heart rate and heart rate variability recordings were performed in home care nurses over a period of one work shift and the following night. Following the measurements, the participants (N = 91) answered a questionnaire including questions about their self-rated recovery from work and sleep disturbances.

Results
The objectively measured recovery was within the recommended heart rate variability range, yet the self-rated recovery from work was only mediocre. Subjective recovery was not associated with objectively measured recovery.

Conclusions
There is a discrepancy between objectively and subjectively measured recovery from work. Therefore, an additional objective method in occupational field studies, along with questionnaires, is recommended to measure physiological recovery.
Parkes Weber Syndrome: Contribution of the Genotype to the Diagnosis
Authors: Andreoti Themis-Areti A. , Tuleja Aleksandra , Döring Yvonne , Maiolo Massimo , Schaller André , Vassella Erik , Zweier Christiane , Boon Laurence M. , Vikkula Miikka , Rössler Jochen , Bernhard Sarah M. , Baumgartner Iris 
Published: November 2023

Objectives:
Parkes Weber syndrome (PWS) is a rare disorder that combines overgrowth, capillary malformations, and arteriovenous malformations (AVM)/arteriovenous fistulas, for which underlying activating mutations in the ras/mitogen-activated protein kinase/extracellular-signal-regulated kinase signaling pathway have been described. The clinical overlap with Klippel-Trenauny syndrome, associated with mutations in PIK3CA, is significant. This case series aimed to elaborate on the phenotypic description of PWS, to underline its clinical overlap with Klippel-Trenauny syndrome and nonsyndromic AVM, and to evaluate the contribution of genotypic characterization to the diagnosis.

Methods:
All patients diagnosed with PWS upon enrollment in the Bernese VAScular COngenital Malformations (VASCOM) cohort were included. The diagnostic criteria of PWS were retrospectively reviewed. A next-generation sequencing (NGS) gene panel (TSO500, Illumina) was used on tissue biopsy samples.

Results:
Overall, 10/559 patients of the VAScular COngenital Malformations cohort were initially diagnosed with PWS. Three patients were reclassified as nonsyndromic AVM (Kristen Rat Sarcoma Viral oncogene homolog [KRAS], KRAS+tumor protein p53, and protein tyrosine phosphatase non-receptor type 11). Finally, 7 patients fulfilled all clinical diagnostic criteria of PWS. Genetic testing was available in 5 PWS patients. Only 1 patient had the classic RASA1 mutation; another patient had mutations in G protein subunit alpha q (GNAQ) and phosphatase and tensin homolog. In a third case, a PIK3CA mutation was detected. In 2 patients, no mutations were identified.

Conclusion:
Overgrowth syndromes with vascular malformations are rare and their clinical overlap hampers the classification of individual phenotypes under specific syndrome labels, sometimes even despite genetic testing. To provide optimal patient care, an accurate phenotypic description combined with the identification of molecular targets for precision medicine may be more meaningful than the syndrome classification itself.
Effect of White Noise on Pain Response, Heart Rate, and Oxygen Saturation During Heel Puncture in Premature Infants
Authors: Kim Min Hye , Song Ju-Eun , Ahn Jeong-Ah , You Mi-Ae 
Published: November 2023

Objective:
This study aimed to investigate the effect of white noise on pain response, heart rate, and oxygen saturation during heel puncture in premature infants.

Methods:
A randomized, controlled, pretest-posttest design was used. The participants were premature infants admitted to the neonatal intensive care unit of a university hospital in Gyeonggi Province. Sixty premature infants were assigned to either an experimental (n = 30) or control (n = 30) group. The experimental group was exposed to white noise during heel puncture, and the measured variables were pain response, heart rate, and oxygen saturation. The data were analyzed using the independent t test, chi-squared test, and analysis of covariance.

Results:
Premature infants in the experimental group had a lower pain response and heart rate than the control group (F = 81.26, P < .01; F = 7.05, P = .01), and higher oxygen saturation than the control group (F = 4.76, P = .03).

Conclusion:
These results demonstrated that the white noise intervention is an effective nursing intervention to reduce the pain response and stabilize heart rate and oxygen saturation in premature infants during heel puncture.
Prongs or Mask for Nasal Continuous Positive Airway Pressure in Neonates
Authors: Arcagok Baran Cengiz , Bilgen Hulya , Memisoglu Asli , Ozdemir Hulya , Sakarya Sibel , Ozek Eren 
Published: November 2023

Background:
Nasal continuous positive airway pressure (NCPAP) is a common mode of respiratory support in neonatal intensive care units. Our objective was to compare whether NCPAP given with nasal prongs compared with a nasal mask reduces the pain scores in preterm infants with respiratory distress.

Methods:
Preterm infants on NCPAP due to respiratory distress were included in the study. All infants received NCPAP via the Infant Flow SiPAP. The COVERS pain scale was used to score the infants' pain. Each infant was studied alternating between nasal prongs and a nasal mask. Heart rate, respiratory rate, oxygen saturation, and transcutaneous CO2 (tcCO2) were monitored. Blood pressure and the infants' pain scores were determined every 30 minutes and the average of measurements was taken.

Results:
The median (interquartile range) values of pain scores, respiratory rates, oxygen saturations, tcCO2 levels, and systolic blood pressures differed significantly and favored the nasal mask.

Conclusions:
This study demonstrates that continuous positive airway pressure via a nasal mask leads to a significant reduction in pain scores without altering the respiratory parameters of babies. On the basis of this study, it is possible to conclude that NCPAP applied via nasal mask may be a good alternative to NCPAP applied via nasal prongs.
Focus Group Study of Heart Failure Nurses’ Perceptions of the Feasibility of Cognitive Behavioral Therapy for Insomnia
Authors: O’Connell Meghan , Feder Shelli L. , Nwanaji-Enwerem Uzoji , Redeker Nancy S. 
Published: November 2023

Background
People with heart failure (HF) often report insomnia with daytime consequences, including fatigue and decreased functional performance. Cognitive behavioral therapy for insomnia is an efficacious treatment, but few have access because of a shortage of trained sleep specialists. Access may be improved by offering it where people with HF receive care.

Objectives
The purpose of this study was to explore the perceptions of nurses who specialize in HF regarding the value of cognitive behavioral therapy for insomnia to their patients, the feasibility of offering it in HF clinical settings, its delivery by nurses, and preferences for modes of delivery.

Methods
We used a descriptive qualitative study design. We recruited focus group participants via e-mail to American Association of Heart Failure Nurses members and through requests for nurse collaborators to distribute within their networks. We conducted focus groups via Zoom. After describing cognitive behavioral therapy for insomnia and its efficacy for people with HF, we elicited perceptions about its value if provided in the HF outpatient clinical setting, facilitators and barriers to implementation, and other ways to increase access. We audio-recorded and transcribed the discussions. Two researchers coded the data and performed thematic analysis.

Results
Four focus groups included 23 registered nurses and advanced practice nurses employed in outpatient HF clinics. We identified five themes: “Insomnia Overlooked,” “Cognitive Behavioral Therapy for Insomnia Works,” “Nurses’ Role,” “Barriers and Supports,” and “Modes of Delivery.” Nurses endorsed the importance of insomnia to people with HF and the value of providing cognitive behavioral therapy. They expressed interest in evaluating and addressing sleep, the need for increased resources to address it, and multiple modes of delivery. All nurses believed they had a role in promoting sleep health but differed in their views about providing cognitive behavioral therapy for insomnia.

Discussion
Nurses specializing in HF support the implementation of cognitive behavioral therapy for insomnia. Implementation studies are needed to identify effective methods to increase access to this efficacious treatment in outpatient HF clinical settings, including support and training for nurses who are interested and able to deliver it.
Delay Between Actual Occurrence of Patient Vital Sign and the Nominal Appearance in the Electronic Health Record: Single-Center, Retrospective Study of PICU Data, 2014–2018
Authors: Schlosser Metitiri Katherine R. , Perotte Adler 
Published: November 2023

OBJECTIVES:Patient vital sign data charted in the electronic health record (EHR) are used for time-sensitive decisions, yet little is known about when these data become nominally available compared with when the vital sign was actually measured. The objective of this study was to determine the magnitude of any delay between when a vital sign was actually measured in a patient and when it nominally appears in the EHR.DESIGN:We performed a single-center retrospective cohort study.SETTING:Tertiary academic children’s hospital.PATIENTS:A total of 5,458 patients were admitted to a PICU from January 2014 to December 2018.INTERVENTIONS:None.MEASUREMENTS AND MAIN RESULTS:We analyzed entry and display times of all vital signs entered in the EHR. The primary outcome measurement was time between vital sign occurrence and nominal timing of the vital sign in the EHR. An additional outcome measurement was the frequency of batch charting. A total of 9,818,901 vital sign recordings occurred during the study period. Across the entire cohort the median (interquartile range [IQR]) difference between time of occurrence and nominal time in the EHR was in hours:minutes:seconds, 00:41:58 (IQR 00:13:42–01:44:10). Lag in the first 24 hours of PICU admission was 00:47:34 (IQR 00:15:23–02:19:00), lag in the last 24 hours was 00:38:49 (IQR 00:13:09–01:29:22;p< 0.001). There were 1,892,143 occurrences of batch charting.CONCLUSIONS:This retrospective study shows a lag between vital sign occurrence and its appearance in the EHR, as well as a frequent practice of batch charting. The magnitude of the delay—median ~40 minutes—suggests that vital signs available in the EHR for clinical review and incorporation into clinical alerts may be outdated by the time they are available.