bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trends in Mortality and Morbidities for Infants Born 24 to 28 Weeks in the US: 1997–2021
Authors: Horbar Jeffrey D. , Greenberg Lucy T. , Buzas Jeffrey S. , Ehret Danielle E.Y. , Soll Roger F. , Edwards Erika M. 
Published: December 2023

BACKGROUND
Mortality and morbidity for very preterm infants in the United States decreased for years. The current study describes recent changes to assess whether the pace of improvement has changed.

METHODS
Vermont Oxford Network members contributed data on infants born at 24 to 28 weeks’ gestation from 1997 to 2021. We modeled mortality, late-onset sepsis, necrotizing enterocolitis, chronic lung disease, severe intraventricular hemorrhage, severe retinopathy of prematurity, and death or morbidity by year of birth using segmented relative risk regression, reporting risk-adjusted annual percentage changes with 95% confidence intervals overall and by gestational age week.

RESULTS
Analyses of data for 447 396 infants at 888 hospitals identified 3 time point segments for mortality, late onset sepsis, chronic lung disease, severe intraventricular hemorrhage, severe retinopathy of prematurity, and death or morbidity, and 4 for necrotizing enterocolitis. Mortality decreased from 2005 to 2021, but more slowly since 2012. Late-onset sepsis decreased from 1997 to 2021, but more slowly since 2012. Severe retinopathy of prematurity decreased from 2002 to 2021, but more slowly since 2011. Necrotizing enterocolitis, severe intraventricular hemorrhage, and death or morbidity were stable since 2015. Chronic lung disease has increased since 2012. Trends by gestational age generally mirror those for the overall cohort.

CONCLUSIONS
Improvements in mortality and morbidity have slowed, stalled, or reversed in recent years. We propose a 3-part strategy to regain the pace of improvement: research; quality improvement; and follow through, practicing social as well as technical medicine to improve the health and well-being of infants and families.
ANÁLISE DA QUALIDADE DE VIDA ENTRE GRADUANDOS DA ÁREA DA SAÚDE DURANTE A PANDEMIA DE COVID-19
Authors: Campos Tainá Lina de , Lima Tamara Aparecida de Oliveira , Ide Patricia Haranaka 
Published: December 2023

A graduação é uma etapa marcada por descobertas, adaptações e mudanças. Essa rotina pode influenciar positivamente ou negativamente, modificando a qualidade de vida (QV) desses graduandos. Objetivo: Analisar a qualidade de vida de graduandos de diferentes cursos da área da saúde. Método: Trata-se de um estudo transversal, descritivo composto por graduandos dos cursos de enfermagem, farmácia e fisioterapia de todos os semestres. Utilizou-se um formulário sociodemográfico para caracterizar a população e o Medical Outcomes Study 36-item Short-Form Health Survey (SF-36), para avaliar a QV desses participantes.
Palavras-chave: Qualidade de Vida; Estudantes de Ciências da Saúde; Universidades.
Educational Technology Undergraduates’ Performance in a Distance Learning Course Using Three Courseware Formats
Authors: Falode Oluwole Caleb , Mohammed Ibrahim Abba 
Published: December 2023

Most educators’ inability to provide learning contents that suit different learning styles has caused a lot of problems in terms of performance. Thus, to cater to students’ preferences in terms of access to learning contents, the distance learning regulatory body in Nigeria emphasized that course materials should be developed in mixed-media formats. This study was carried out to compare the effects of printed, video, and Moodle-based courseware on educational technology students’ achievement, retention, and satisfaction in a distance learning course. A quasi-experimental design was employed for the study involving 108 participants from three experimental groups. The learning content and instruments, subjected to validation and reliability tests, where values of 0.78 and 0.86 were obtained using the Pearson product moment correlation and Cronbach’s alpha for achievement and satisfaction inventory, respectively, were administered within a four-week period. Data collected were analyzed using descriptive and inferential statistics. Findings indicated that the printed, video, and Moodle-based courseware formats improved students’ achievement with mean gain scores of 47.92, 40.89, and 43.03, respectively. A significant difference was observed in the achievement (F (2,104) = 8.67, p < 0.05), retention (F (2,104) = 29.406, p < 0.05), and satisfaction scores (F (2,104) = 5.662, p < 0.05) of the three groups. Open and distance learning administrators in Nigeria are recommended to produce and deploy printed, video, and Moodle-based formats of courseware to meet different students’ learning preferences.
The Urgency of Karyotyping Examination in Male Infertility Patients with Primary Hypogonadism
Authors: Putu Dyah Agustina Lestari  , I Gusti Ngurah Pramesemara  
Published: December 2023

Infertility is a failure to get pregnant after one year of sexual intercourse without using contraception. The causes of infertility, especially in men, are very complex, including the aging process, hormonal disorders, lifestyle, environment, metabolic disease, and genetic problems. One of the most difficult causes in male infertility is genetic problems.
In this case, a 35 year old man was reported with primary infertility and often experienced premature ejaculation and even anejaculation. Previously, the patient had repeatedly consulted about his condition at other fertility service centers. The conclusion obtained was that the patient had azoospermia with bilateral varicoceles and had been given hormone therapy but had not yet found a final diagnosis so the patient was still confused about what had happened to him. At the first visit the patient underwent a sperm analysis and the results were azoospermia. Then, based on the results of the history and physical examination, which indicated hypogonadism, a Y chromosome microdeletion examination was carried out, and a deletion was found in the AZFc region, which is a marker of infertility that causes spermatogenic failure. The examination was continued with karyotyping, the result was 47.XXY, consistent with the condition of Klinefelter syndrome.
Conclusion: Carry out a karyotyping examination if you find signs and symptoms that suggest primary hypogonadism in male infertility. This can be done to streamline the diagnostic approach time in patients with primary hypogonadism, especially those with infertility.
Kepastian Hukum Dalam Permohonan Kewarganegaraan Republik Indonesia Secara Elektronik
Authors: Dimas Tri Sunu  
Published: December 2023

Abstract
Since the issuance of the Regulation of the Minister of Law and Human Rights Number 47 of 2016 regarding Procedures of Indonesian Citizenship Application through an Electronic System, people can apply citizenship services through an application called SAKE. Using SAKE surely can simplify and accelerate the citizenship services process in Indonesia. The writing of this article uses the normative method and uses the conceptual method as well. And based on these method, there were several problems where the applicants that have right to be able to submit their application were not regulated as applicants in the Regulation. Also, the Regulation had not regulated regarding the supervision performed by the government on SAKE. Therefore, the government must carry out maximum law enforcement and supervision so that existing regulations can be maintained and improved.
Keywords: Citizenship Application; Electronic System; SAKE; Government Supervision.
Abstrak
Sejak diterbitkannya Peraturan Menteri Hukum dan Hak Asasi Manusia Nomor 47 Tahun 2016 tentang Tata Cara Permohonan Kewarganegaraan Indonesia Melalui Sistem Elektronik, masyarakat dapat mengajukan layanan kewarganegaraan melalui aplikasi bernama SAKE. Penggunaan SAKE tentunya dapat mempermudah dan mempercepat proses layanan kewarganegaraan di Indonesia. Penulisan artikel ini menggunakan metode normatif dan menggunakan metode konseptual pula. Dan berdasarkan cara tersebut, terdapat beberapa permasalahan dimana pemohon yang mempunyai hak untuk dapat mengajukan permohonannya tidak diatur sebagai pemohon dalam Peraturan tersebut. Selain itu, peraturan tersebut juga belum mengatur mengenai pengawasan yang dilakukan pemerintah terhadap SAKE. Oleh karena itu, pemerintah harus melakukan penegakan hukum dan pengawasan secara maksimal agar peraturan yang ada dapat dipertahankan dan diperbaiki.
​Kata Kunci: Permohonan Kewarganegaraan; Secara Elektronik; SAKE; Pengawasan Pemerintah;
Biochemical and morphological basis of resistance in okra (Abelmoschus esculentus L.) against whitefly and jassid
Authors: Barman Mritunjoy , Samanta Snigdha , Atta Kousik , Dutta Subham , Dey Soumik , Samanta Arunava , Tarafdar Jayanta , Ahmed Bulbul 
Published: December 2023

Abstract

A major constraint in okra production is damage caused by insect pests, whitefly and jassids, which results in low yield. Identification of resistant varieties is a convenient and eco-friendly way towards sustainable management of insect pests attacking okra. Therefore, population dynamics of the two major pests were recorded in fifteen hybrid varieties of okra during two consecutive years in 2019 and 2020. Significant varietal difference in terms of insect infestation were recorded with, Kajari, NOH-1684, Japani jhar, Singham and Rohini being comparatively resistant varieties. Correlation studies revealed significant negative correlation of whitefly and jassid population with hair density, hair length and total phenols; positive correlation with total protein and total sugar content. The comparative analysis of superoxide dismutase and peroxidase activity revealed varieties more susceptible to whitefly and jassid attack resulted in higher enzyme activity in all the growth stages. The genotypes Kajari NOH-1684, Japani jhar, Singham and Rohini exhibit low infestations of whitefly and jassid, suggesting a tolerance based on their antixenotic properties. To enhance tolerance against these sucking pests, it is recommended to introduce the donor parents Kajari NOH-1684, Japani jhar, Singham and Rohini into okra breeding programs.
Dreaming during gastrointestinal endoscopy under propofol, ciprofol or remimazolam anesthesia: study protocol for a randomized, controlled, single-center trial
Authors: Zhou Rui , Xia Leqiang , Deng Rui , Zhou Dan , Han Jia , Gao Sanjie , Zhao Zhifu , Zhou Yukai , Zhang Xianjie , Xiong Lize 
Published: December 2023

Abstract

BackgroundDreaming sometimes occurs during sedation. It has been reported that factors such as different anesthetics, depth of anesthesia, age, sex, and preoperative psychological state may affect dreams. Ciprofol and remimazolam are novel choices for painless endoscopy. Herein, we aimed to investigate dreaming during gastrointestinal endoscopy under propofol, ciprofol and remimazolam anesthesia respectively.MethodsThis is a prospective, randomized, controlled, single-center clinical trial.Three hundred and sixty subjects undergoing elective painless gastroscopy, colonoscopy or gastroenteroscopy will be enrolled. Eligible subjects will undergo propofol-, ciprofol- or remimazolam-induced anesthesia to finish the examination. Interviews about the modified Brice questionnaire will be conducted in the recovery room. Incidence of dreaming is set as the primary outcome. Secondary outcomes include type of dreams, improvement of sleep quality, evaluation of patients, incidence of insufficient anesthesia and intraoperative awareness. Safety outcomes are the incidences of hypotension and hypoxia during examination and adverse events during recovery.DiscussionThis study may observe different incidences of dreaming and diverse types of dreams, which might lead to different evaluationsto the anesthesia procedure. Based on the coming results, anesthesiologists can make a better medication plan for patients who are going to undergo painless diagnosis and treatment.Trial registrationThis trial was registered at the Chinese Clinical Trial Registry on May 18, 2023 (registration number ChiCTR2300071565).
Residue Evaluation of Spiromesifen in Tomato fruits, Leaves, and Soil in Baghdad-Iraq using QuEChERS Extraction via FID-Gas Chromatography
Authors: Kadhum Hanan Abdulsalam , Hammood Mohammad K. , Arif Mokhtar Abdulsattar 
Published: December 2023

Abstract

Spiromesifen is an insecticide used to control whiteflies, red spider mites, and other pests in agriculture. This study has investigated a selective gas chromatography (GC) analytical method to determine spiromesifen residue levels in tomato fruits, leaves, and soil. Samples were extracted and cleaned utilizing a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method prior to GC analysis. Validation studies were performed by spiking blank tomato fruits, leaves, and soil samples at 0.5, 1, and 10 µg/mL spiromesifen. Satisfactory recoveries were achieved with percentage recoveries of 98.74%, 93.92%, and 94.18% for fruits, leaves, and soil, respectively. Negligible matrix effects were observed, with values of -7.9%, -7.8%, and − 5.3% for fruits, leaves, and soil correspondingly. The method exhibited strong linearity across a spiromesifen concentration range of 0.002-20 µg/mL, with R2 values exceeding 0.9900. Repeatability was also demonstrated via relative standard deviations of < 3%. Confirmatory studies were performed via GC–MS in selective ion monitoring mode within a mass range of 271–274 (m/z). Under greenhouse conditions, spiromesifen residues on the tomato fruits, leaves and soil were 3.39, 8.24 and 1.39 mg.kg− 1, respectively. The dissipation of spiromesifen residues followed first-order rate kinetics and degraded within a dissipation time of 50% of 1.6 days. This study was indicates that the proposed GC analytical methodology enables selective, accurate, and precise quantification of spiromesifen residues in tomato fruits, leaves, and soil. The method could be utilized for monitoring spiromesifen levels in agricultural environments to ensure compliance with insecticide regulations.
Genome-wide identification and expression analysis of the SET domain-containing gene family in potato (Solanum tuberosum L.)
Authors: Suppiyar Vithusan , Bonthala Venkata Suresh , Shrestha Asis , Krey Stephanie , Stich Benjamin 
Published: December 2023

Abstract

Genes containing the SET domain can catalyse histone lysine methylation, which in turn has the potential to cause changes to chromatin structure and regulation of the transcription of genes involved in diverse physiological and developmental processes. However, the functions of SET domain-containing (StSET) genes in potato still need to be studied. The objectives of our study were to (i) identify StSET genes in the potato genome, (ii) systematically analyse gene structure, chromosomal distribution, gene duplication events, promoter sequences, and protein domains, (iii) perform phylogenetic analyses, (iv) compare the SET domain-containing genes of potato with other plant species with respect to protein domains and orthologous relationships, (v) analyse tissue-specific expression, and (vi) study the expression of StSET genes in response to drought and heat stresses. In this study, we identified 57 StSET genes in the potato genome, and the genes were physically mapped onto eleven chromosomes. The phylogenetic analysis grouped these StSET genes into six clades. We found that tandem duplication through sub-functionalisation has contributed only marginally to the expansion of the StSET gene family. The protein domain TDBD (PFAM ID: PF16135) was detected in StSET genes of potato while it was absent in all other previously studied species. This study described three pollen-specific StSET genes in the potato genome. Expression analysis of four StSET genes under heat and drought in three potato clones revealed that these genes might have non-overlapping roles under different abiotic stress conditions and durations. The present study provides a comprehensive analysis of StSET genes in potatoes, and it serves as a basis for further functional characterisation of StSET genes towards understanding their underpinning biological mechanisms in conferring stress tolerance.
Predicting Monoclonal Antibody Binding Sequences from a Sparse Sampling of All Possible Sequences
Authors: Woodbury Neal , Bisarad Pritha , Kelbauskas Laimonas , Singh Akanksha , Taguchi Alexander 
Published: December 2023

Abstract

A general approach is described for creating a comprehensive and quantitative model for predicting the binding of a monoclonal antibody (mAb) to any short linear sequence of amino acids. Binding of 8 different mAbs with continuous epitopes to a common library of ~122,000 near-random peptide sequences arrayed on a silica substrate was used to train neural network models resulting in a general sequence-binding relationship for each mAb. The model was then used to predict mAb binding to 1,000,000 randomly generated sequences as well as the cognate sequence of each mAb. In every case the cognate sequence ranked in the top 250 sequences, and for 5 of the 8 mAbs it ranked in the top 10 out of one million. Practically, this approach has potential utility in selecting highly specific mAbs for therapeutics or diagnostics. Conceptually, this demonstrates that very sparse, but near random, sampling of a large sequence space is sufficient to generate comprehensive models predictive of highly specific molecular recognition.
Beyond Disaster: Investigating the Varied Responses of Regional Entrepreneurship to Natural Disasters
Authors: Demirdag Ismail , Nirwansyah Anang Widhi 
Published: December 2023

Abstract

Natural disasters can cause large-scale damage to various infrastructures, preventing sustainable industrialization, innovation activities, and economic growth and prosperity. This article, which tries to examine to what extent entrepreneurial activities, which are considered the critical source of new businesses, employment, innovations and economic development, are affected by natural disasters and how the level of impact differs by region, offers remarkable results. First, it employs Hierarchical Multiple Regression (HMR) analysis to investigate the impact of natural disasters on regional entrepreneurship, considering various factors including supply side, demand side, political and environmental factors. An important finding of the research is that loss of human life, rather than physical damage, emerges as a critical factor shaping regional entrepreneurship development. Second, the study utilizes Discriminant Function Analysis (DFA) to identify regional determinants that contribute to the resilience of entrepreneurship in the face of natural disasters. The research highlights the significance of regional features, government policies and environmental conditions in shaping regional entrepreneurial resilience. Future research avenues could investigate the regional impact of specific types of disasters, conduct subsector-specific analyses, and investigate the role of institutional quality in mitigating the effects of disasters at the regional level.
DNMT3L Inhibits Hepatocellular Carcinoma Progression through DNA Methylation of CDO1: Insights from Big Data to Basic Research
Authors: Yan Xiaokai , Qi Yao , Yao Xinyue , Zhou Nanjing , Ye Xinxin , Chen Xing 
Published: December 2023

Abstract
Background
DNMT3L is a crucial DNA methylation regulatory factor, yet its function and mechanism in hepatocellular carcinoma (HCC) remain poorly understood. Bioinformatics-based big data analysis has increasingly gained significance in cancer research. Therefore, this study aims to elucidate the role of DNMT3L in HCC by integrating big data analysis with experimental validation.
Methods
Dozens of HCC datasets were collected to analyze the expression of DNMT3L and its relationship with prognostic indicators, and were used for molecular regulatory relationship evaluation. The effects of DNMT3L on the malignant phenotypes of hepatoma cells were confirmed in vitro and in vivo. The regulatory mechanisms of DNMT3L were explored through MSP, western blot, and dual-luciferase assays.
Results
DNMT3L was found to be downregulated in HCC tissues and associated with better prognosis. Overexpression of DNMT3L inhibits cell proliferation and metastasis. Additionally, CDO1 was identified as a target gene of DNMT3L and also exhibits anti-cancer effects. DNMT3L upregulates CDO1 expression by competitively inhibiting DNMT3A-mediated methylation of CDO1 promoter.
Conclusions
Our study revealed the role and epi-transcriptomic regulatory mechanism of DNMT3L in HCC, and underscored the essential role and applicability of big data analysis in elucidating complex biological processes.
Comparison of models for predicting intracranial pressure by optic nerve sheath diameter in combination with other indicators: A study based on ultrasound measurements
Authors: cheng Mingqing , Liu Tingting , Guo Geng , Hui Zili , Zhang Lei , Hu Jianqiang , Zhang Jing , Fang Yalan 
Published: December 2023

Abstract
Introduction:
The optic nerve sheath diameter (ONSD) assessment of elevated intracranial pressure is increasingly emphasized as a noninvasive modality. Still, quantitative assessment is less common in current studies, and study criteria are variable. We constructed four prediction models related to ONSD and compared their accuracy in predicting intracranial pressure (ICP).
Methods
This study included 223 patients who completed lumbar puncture (LP) at the First Hospital of Shanxi Medical University with values of ICP and Mindray-M58 ultrasound-measured ONSD, optic nerve sheath (OND), and eyeball transverse diameter (ETD). The patients were randomly assigned 7:3 to model and test groups, and predictive models (ONSD model, ONSD combined OND model, ΔONSD model, and ONSD/ETD model) were established in the model group using linear regression analysis. The test group evaluated the agreement between the models' predicted and actual ICP values using Bland‒Altman analysis and the intragroup correlation coefficient (ICC).
Results
ICP values were strongly correlated with ΔONSD, ONSD, and ONSD/ETD. The ONSD combined OND model showed the highest predictive power for increased ICP (area under the receiver operating characteristic curve [AUC] = 0.933). The four mathematical equations established in the model group (n = 154) were as follows: 1. ONSD combined OND model: predicted ICP = 139.394×ONSD-112.428×OND-267.461; 2. ONSD model: predicted ICP = 108.227× ONSD-404.446; 3. ΔONSD model: predicted ICP = 140.216 × ΔONSD-196.335; 4. ONSD/ETD model: predicted ICP = 1624.283×ONSD/ETD-196.904. In the test group (n = 66), it was shown that ONSD combined OND (ICC = 0.88) prediction accuracy was the highest.
Conclusion
Bedside ultrasound measurement of ONSD combined OND is a practical method to assess ICP values. This study indicated that the efficacy of the ONSD combined with the OND model and the ΔONSD model in predicting ICP was better than that of ONSD alone, reflecting the fact that intracranial pressure changes mainly affect the optic nerve sheath rather than the optic nerve.