bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous multislice imaging with slice‐multiplexed RF pulses
Authors: Lee K.J. , Wild J.M. , Griffiths P.D. , Paley M.N.J. 
Published: September 2005

AbstractA method for simultaneous multislice imaging is presented that uses a multislice RF pulse that imparts a different linear phase profile to each slice. During readout, slices are unaliased by using extra slice‐select gradient lobes, which rephase and dephase individual slices one at a time. Compared to other simultaneous slice methods, this method avoids distortion by slice‐select gradients, and does not require extra views or additional hardware. However, because one echo per slice is required, the method requires a longer read period. This can cause non‐ideal rephasing of the individual slices due to susceptibility gradients, which manifests itself as crosstalk between slices. There is also a concomitant increase in the minimum TR of the sequence. The method is demonstrated with phantom and in vivo images using gradient‐echo and spin‐echo versions. Magn Reson Med, 2005. © 2005 Wiley‐Liss, Inc.
Experimental analysis of parallel excitation using dedicated coil setups and simultaneous RF transmission on multiple channels
Authors: Ullmann Peter , Junge Sven , Wick Markus , Seifert Frank , Ruhm Wolfgang , Hennig Jürgen 
Published: September 2005

AbstractAn experimental implementation and first performance analysis of parallel spatially selective excitation with an array of transmit coils and simultaneous transmission of individual waveforms on multiple channels is presented. This technique, also known as Transmit SENSE, uses the basic idea of parallel imaging to shorten the k‐space trajectories that accompany multidimensional excitation pulses, and hence shorten the duration of such pulses. So far, this concept has only been presented in simulations and semi‐experimental studies since no hardware setup had been available for a full experimental realization. In this study, a hardware solution, in combination with a dedicated coil setup, is presented to overcome this limitation, and in several experiments of localized excitation and transmit field inhomogeneity compensation the practical feasibility of Transmit SENSE is demonstrated and a first performance analysis is given. Magn Reson Med, 2005. © 2005 Wiley‐Liss, Inc.
Indicative oligodendrocyte dysfunction in spinal cords of human fetuses suffering from a lethal motoneuron disease
Authors: Pakkasjärvi Niklas , Gentile Massimiliano , Saharinen Juha , Honkanen Jarno , Herva Riitta , Peltonen Leena , Kestilä Marjo 
Published: September 2005

AbstractHuman spinal cord development is still poorly understood and detailed molecular analyses of human motoneuron diseases could improve our understanding of the normal developmental processes of the spinal cord. Lethal Congenital Contracture Syndrome (LCCS, MIM 253310) provides a human model to study the early motoneuronal development. A typical phenotype of LCCS fetuses consists of multiple joint contractures, distinct facial features, and hydrops. Tissue pathology is characterized by severe muscle atrophy, lung hypoplasia, and degeneration of the anterior horn of the spinal cord as the hallmark of the syndrome. In this study we performed a global transcript analysis of LCCS spinal cords. The RNA expression profiles of these spinal cords were compared to age‐matched healthy control fetuses, aborted for nonrelated causes. In addition, we applied phylogenetic footprinting methods to decipher the mechanisms of transcriptional regulation in the affected transcripts. Changes in transcripts involved with the development of the CNS and oligodendrocytes were obvious and the transcription factor PAX6 was identified as a key regulator during spinal cord development. In addition, transcript pathway analysis clearly indicated genes belonging to groups with neuronal functions to be affected. Our findings support the hypothesis that human motoneurons and oligodendrocytes are dependent on each other during their development and are influenced by distinct transcription factors previously known to act during murine and chick motoneuron development. These data provide valuable information about the molecular pathways putatively active in motoneuron diseases. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005
Isolation and culture of hair cell progenitors from postnatal rat cochleae
Authors: Zhai Suoqiang , Shi Li , Wang Bu‐er , Zheng Guiliang , Song Wei , Hu Yinyan , Gao Wei‐Qiang 
Published: September 2005

AbstractCochlear hair cells are a terminally differentiated cell population that is crucial for hearing. Although recent work suggests that there are hair cell progenitors in postnatal mammalian cochleae, isolation and culture of pure hair cell progenitors from a well‐defined cochlear area have not been reported. Here we present an experimental method that allows isolation and culture of hair cell progenitors from postnatal rat cochleae. These progenitor cells are isolated from the lesser epithelial ridge (LER, or outer spiral sulcus cell) area of pre‐plated neonatal rat cochlear segments. They express the same markers as LER cells in vivo, including ZO1, Islet1, Hes1, and Hes5. When these cells are induced to express Hath1, they show the potential to differentiate into hair cell‐like cells. Interestingly, these cells can be lifted from monolayer cultures and maintained in aggregate cultures in which spheres can be formed. Hair cell progenitors in the spheres display their proliferating capability and express only epithelial markers. Furthermore, when these spheres are mixed with dissociated mesenchymal cells prepared from postnatal rat utricular whole mounts, and replated onto a collagen substratum, the epithelial progenitor cells are able to differentiate into cells expressing markers of hair cells and supporting cells in epithelial islands, which mirrors the inner ear sensory epithelium in vivo. Successful isolation and culture of hair cell progenitors from the mammalian cochlea will facilitate studies on gene expression profiling and mechanism of differentiation/regeneration of hair cells, which are crucial for repairing hearing loss. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005
Extramedullary plasmacytoma of the adenoids
Authors: Mann Georg , Trebo Monika M. , Minkov Milen , Simonitsch Ingrid , Chott Andreas , Gadner Helmut 
Published: September 2005

AbstractExtramedullary plasmacytomas are extremely rarely diagnosed in children. We report two cases of extramedullary plasmacytoma detected coincidentally in the adenectomy specimens of children younger than 4 years. We show that these children are disease‐free after local excision as the only treatment. Surgical treatment without anti‐neoplastic therapy in pediatric plasma cell dyscrasias of the pharyngeal lymphoid tissue may be a sufficient curative therapy. Pediatr Blood Cancer 2007;48:361–362. © 2005 Wiley‐Liss, Inc.
Primary renal lymphoma presenting with hypertension
Authors: Becker Amy M. , Bowers Daniel C. , Margraf Linda R. , Emmons Jacqueline , Baum Michel 
Published: September 2005

AbstractPrimary renal lymphoma (PRL) is a rare lymphoma which usually presents with hematuria, flank pain, abdominal mass, and weight loss. PRL is more diagnosed in adults than children. We describe an asymptomatic child who presented with hypertension and was subsequently diagnosed with primary renal lymphoma. This case represents an atypical presentation for PRL. Pediatr Blood Cancer 2007;48:711–713. © 2005 Wiley‐Liss, Inc.
Outcome of hematopoietic stem cell transplantation for pediatric patients with therapy‐related acute myeloid leukemia or myelodysplastic syndrome
Authors: Woodard Paul , Barfield Raymond , Hale Gregory , Horwitz Edwin , Leung Wing , Ribeiro Raul , Rubnitz Jeffrey , Srivistava Deo Kumar , Tong Xin , Yusuf Usman , Raimondi Susana , Pui Ching‐Hon , Handgretinger Rupert , Cunningham John M. 
Published: September 2005

AbstractBackgroundTherapy‐related myelodysplastic syndrome (t‐MDS) and acute myeloid leukemia (t‐AML) carry a poor prognosis. We analyzed the results of allogeneic HSCT in 38 children to determine which factors, if any, affected outcome.ProcedureThe effects of demographic, donor, and disease‐related factors were analyzed to determine their effects on overall and disease‐free survival (OS, DFS), relapse, and non‐relapse mortality (NRM).ResultsOS and DFS for t‐AML and t‐MDS were similar. Three‐year OS and EFS were the same (15.4 ± 5.8%) and the 3‐year NRM was 59.6 ± 8.4%. The 1‐year cumulative risk of grade III–IV acute graft‐versus‐host disease (GVHD) and relapse were 23.7 ± 7.0% and 18.7 ± 6.5%, respectively. The percentage of pre‐transplant bone marrow (BM) blasts was positively associated with relapse (P = 0.05), while the percentage of BM blasts at diagnosis of therapy‐related disease tended to associate with NRM (P = 0.07). Alternative donor and matched sibling donor grafts had similar outcomes. NRM was higher among patients who did not develop acute GVHD as compared to those with grade 1–2 acute GVHD (69.2 ± 14.2% vs. ± 12.7%, respectively), while NRM was 100% in patients with grade III–IV acute GVHD (P = 0.007).ConclusionsThe percentage of BM blasts is associated with relapse in these disorders. High rates of NRM negatively impact the outcome of allogeneic HSCT for children with t‐AML and t‐MDS. Future studies should focus on reducing NRM. © 2005 Wiley‐Liss, Inc.
Faisalabad histiocytosis mimics Rosai‐Dorfman disease: Brothers with lymphadenopathy, intrauterine fractures, short stature, and sensorineural deafness
Authors: Rossbach Hans‐Christoph , Dalence Carlos , Wynn Tung , Tebbi Cameron 
Published: September 2005

AbstractRosai‐Dorfman disease (RDD) is a rare, sporadic histiocytic disorder characterized by painless but protracted lymphadenopathy. Its etiology remains unclear. The observation of congenital disease and reports of familial cases with seven pairs of siblings including three sets of identical twins suggests a genetic predisposition in some patients with this condition. We now report two brothers of consanguineous Palestinian parents, whose lymphadenopathy, lymph node histology, and polyclonal hypergammaglobulinemia indicated RDD. The presence of intrauterine fractures, short stature, and sensorineural hearing impairment suggested a rare familial form of the disorder. Moynihan et al. recently described a Pakistani family with a familial histiocytic disorder highly reminiscent of the brothers reported here, whose lymph node morphology was apparently consistent with RDD as well. The presence of sensorineural deafness, short stature, and joint contractures, however, suggested a separate, rare autosomal recessive syndrome referred to as Faisalabad histiocytosis, after the family's place of origin. We believe that the brothers described here represent a second family with Faisalabad histiocytosis, which mimics RDD histologically. Pediatr Blood Cancer 2006; 47:629–632. © 2005 Wiley‐Liss, Inc.
Proteomic analysis of proteins expressed by Helicobacter pylori under oxidative stress
Authors: Chuang Ming‐Hong , Wu Ming‐Shiang , Lin Jou‐Town , Chiou Shyh‐Horng 
Published: September 2005

AbstractHelicobacter pylori is a spiral, slow growing gram‐negative microaerophilic bacterium. It has been shown to be the etiological agent of gastroduodenal diseases, such as chronic gastritis, gastric and duodenal ulcers, and gastric cancer. To address the influence of oxidative stress and its underlying mechanisms, we have compared proliferation, urease activity and protein expression profile of H. pylori incubated under normal microaerophilic (5% O2) and aerobic stress (20% O2) conditions. Oxidative‐stress cells displayed coccoid morphology and time‐dependent decrease in proliferation. The urease activity was completely abrogated after 32 h. We have further compared the protein expression profiles of H. pylori under normal growing and oxidative‐stress conditions by a global proteomic analysis, which includes high‐resolution 2‐DE followed by MALDI‐TOF‐MS and bioinformatic databases search/peptide‐mass comparison. The results revealed that more than ten proteins were differentially expressed under oxidative stress. Most notably, the protein expression levels of urease accessory protein E (UreE, an essential metallochaperone for urease activity) and alkylhydroperoxide reductase (AhpC) with antioxidant potential are greatly decreased under stress conditions. Measurements of messenger RNA transcription level by performing RT‐PCR on total mRNA also confirmed that gene expressions for these two proteins are consistently repressed under oxygen tension. These changes form a firm basis to account for the loss of urease activity and anti‐oxidative ability of H. pylori after long‐term exposure to reactive oxygen. Conceivably, UreE and AhpC may thus be listed as potential targets for the development of therapeutic drugs against H. pylori.
Plasma membrane‐focused proteomics: Dramatic changes in surface expression during the maturation of human dendritic cells
Authors: Watarai Hiroshi , Hinohara Atsushi , Nagafune Jun , Nakayama Toshinori , Taniguchi Masaru , Yamaguchi Yasunori 
Published: September 2005

AbstractThe differential expression of surface molecules on dendritic cells (DC) reflects their functional differences as immature and mature subsets. It is difficult, however, to characterize differences in surface expression by standard proteomic approaches, due mainly to the hydrophobic nature and low abundance of the individual proteins in question. We have established a method for obtaining high‐yield plasmalemma preparations which contain surface molecules enriched more than 200‐fold by coating cells with beads conjugated with antibody against a cell type‐specific cell‐surface molecule, followed by nitrogen cavitated disruption, magnetic separation, and density gradient ultracentrifugation. We identified and quantified 339 human monocyte‐derived DC transmembrane proteins, including 33 previously uncharacterized molecules. Whereas 106 proteins were selectively expressed in immature cells or down‐regulated after maturation, 191 proteins were selectively expressed in mature cells or up‐regulated after maturation.
Wheat cultivar‐specific proteins in grain revealed by 2‐DE and their application to cultivar identification of flour
Authors: Yahata Eriko , Maruyama‐Funatsuki Wakako , Nishio Zenta , Tabiki Tadashi , Takata Kanenori , Yamamoto Yoshihiko , Tanida Masatoshi , Saruyama Haruo 
Published: September 2005

AbstractWheat flour proteins were studied to identify the cultivar‐specific proteins and use them to identify cultivars in flours. Proteins extracted from flours of Japanese wheat (cultivars Hokushin, Horoshirikomugi, Kitanokaori and Kachikei 33) and Canadian wheat (Canada Western Red Spring Wheat No. 1; 1CW) were analyzed by 2‐DE with IEF gels over three pH ranges: pH 4–7, pH 5–8, and pH 6–11. This system enabled detection of more than 1600 protein spots. We recognized that among 50 protein spots showing cultivar‐dependent qualitative changes, 25 proteins were wheat cultivar specific. These 50 protein spots were analyzed by N‐terminal Edman degradation microsequencing and MALDI‐TOF‐MS; 21 protein spots were storage proteins, such as gliadin and low‐molecular mass glutenin subunit. Five protein spots were identified as dehydroascorbate reductase (Triticum aestivum), triticin precursor (T. aestivum), alpha‐amylase inhibitor (Oryza sativa), DNA‐binding with one finger (Dof) zinc family protein (O. sativa), and nonphototropic hypocotyl 1 (NPH1) protein (Avena sativa). The other protein spots appeared to be hypothetical proteins (O. sativa or Arabidopsis thaliana) or functional unknown proteins. These specific proteins can be used as markers to identify wheat cultivars in blended flour composed of two or three flours.
Poly(N‐phenylmaleimide‐co‐acrylic acid)–copper(II) and poly(N‐phenylmaleimide‐co‐acrylic acid)–cobalt(II) complexes: Synthesis, characterization, and thermal behavior
Authors: Marambio O. G. , Pizarro G. del C. , Jeria‐Orell M. , Huerta M. , Olea‐Azar Claudio , Habicher W. D. 
Published: September 2005

AbstractThe free‐radical copolymerization of N‐phenylmaleimide (N‐PhMI) with acrylic acid was studied in the range of 25–75 mol % in the feed. The interactions of these copolymers with Cu(II) and Co(II) ions were investigated as a function of the pH and copolymer composition by the use of the ultrafiltration technique. The maximum retention capacity of the copolymers for Co(II) and Cu(II) ions varied from 200 to 250 mg/g and from 210 to 300 mg/g, respectively. The copolymers and polymer–metal complexes of divalent transition‐metal ions were characterized by elemental analysis, Fourier transform infrared, 1H NMR spectroscopy, and cyclic voltammetry. The thermal behavior was investigated with differential scanning calorimetry (DSC) and thermogravimetry (TG). The TG and DSC measurements showed an increase in the glass‐transition temperature (Tg) and the thermal stability with an increase in the N‐PhMI concentration in the copolymers. Tg of poly(N‐PhMI‐co‐AA) with copolymer composition 46.5:53.5 mol % was found at 251 °C, and it decreased when the complexes of Co(II) and Cu(II) at pHs 3–7 were formed. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 4933–4941, 2005
Effect of alkyl substituents structures and added ions on the phase transition of polymers and gels prepared from methyl 2‐alkylamidoacrylates
Authors: Mori Takeshi , Hamada Masafumi , Kobayashi Toru , Okamura Hirokazu , Minagawa Keiji , Masuda Seizo , Tanaka Masami 
Published: September 2005

AbstractThe effects of structure alteration of alkyl groups and addition of ions to solutions of new thermosensitive polymer series of poly(methyl 2‐alkylamidoacrylate)s were investigated. Potential advantages of the thermosensitive polymers are their simplicity of functionalization and transition temperature control that result from their unique α,α‐disubstituited structures. Poly(methyl 2‐propionamidoacrylate) (PMPA) and poly(methyl 2‐isobutyracrylate) (PMIBA) were thermosensitive polymers, and poly(methyl 2‐acetamidoacrylate) and poly(methyl 2‐n‐butyramidoacrylate) were completely water‐soluble and ‐insoluble, respectively. The PMIBA solution showed endotherm during the phase transition, while endotherm was not detected for PMPA. The difference between the two polymers resulted from the size of the hydrophobic groups. MPA gel was prepared by copolymerization with N,N′‐methylenebis(acrylamide) and temperature‐induced volume change of the gel was continuous. The salting‐out effect of inorganic ions on PMPA solution and MPA gel followed the Hofmeister series. The inorganic (I− and SCN−) and organic ions (nPr4N+ and nBu4N+), which showed the salting‐in effect, were indicated to directly interact with PMPA chains. These ions widened the temperature range of the phase transition of the PMPA solutions. This reduced cooperativity of the phase transition was caused by size decrease of cooperative domains, which resulted from the interaction of the ions with the polymer. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 4942–4952, 2005
Towards a full reference library of MSn spectra. Testing of a library containing 3126 MS2 spectra of 1743 compounds
Authors: Milman Boris L. 
Published: September 2005

AbstractA library consisting of 3766 MSn spectra of 1743 compounds, including 3126 MS2 spectra acquired mainly using ion trap (IT) and triple‐quadrupole (QqQ) instruments, was composed of numerous collections/sources. Ionization techniques were mainly electrospray ionization and also atmospheric pressure chemical ionization and chemical ionization. The library was tested for the performance in identification of unknowns, and in this context this work is believed to be the largest of all known tests of product‐ion mass spectral libraries. The MS2 spectra of the same compounds from different collections were in turn divided into spectra of ‘unknown’ and reference compounds. For each particular compound, library searches were performed resulting in selection by taking into account the best matches for each spectral collection/source. Within each collection/source, replicate MS2 spectra differed in the collision energy used. Overall, there were up to 950 search results giving the best match factors and their ranks in corresponding hit lists. In general, the correct answers were obtained as the 1st rank in up to 60% of the search results when retrieved with (on average) 2.2 ‘unknown’ and 6.2 reference replicates per compound. With two or more replicates of both ‘unknown’ and reference spectra (the average numbers of replicates were 4.0 and 7.8, respectively), the fraction of correct answers in the 1st rank increased to 77%. This value is close to the performance of established electron ionization mass spectra libraries (up to 79%) found by other workers. The hypothesis that MS2 spectra better match reference spectra acquired using the same type of tandem mass spectrometer (IT or QqQ) was neither strongly proved nor rejected here. The present work shows that MS2 spectral libraries containing sufficiently numerous different entries for each compound are sufficiently efficient for identification of unknowns and suitable for use with different tandem mass spectrometers. Copyright © 2005 John Wiley & Sons, Ltd.
A strategy for distinguishing modified peptides based on post‐digestion 18O labeling and mass spectrometry
Authors: Sun Gang , Anderson Vernon E. 
Published: September 2005

AbstractThe simultaneous identification of multiple different protein modifications, with or without known mass changes, is a challenging application of mass spectrometry. In this contribution, a strategy for distinguishing modified peptides within a large background of unmodified peptides was demonstrated by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) analysis of cytochrome c (Cyt‐c) modified with 4‐hydroxy‐2‐nonenal (HNE), based on post‐digestion 18O labeling. Labeling of control Cyt‐c peptides obtained from in‐solution or in‐gel digestion with 18O, prior to mixing in the ratio of 1:1 with peptides derived from a modified sample, identified more HNE modifications than a method based on a known mass increment search (Isom AL, Barnes S, Wilson L, Kirk M, Coward L, Darley‐Usmar V. J. Am. Soc. Mass Spectrom. 2004; 15: 1136), demonstrating the potential of this strategy to enhance the detection of modified peptides by mass spectrometry. A virtue of the strategy is that it obviates the need for isotopic labeling of the modifier, making the method applicable to the detection of modifications occurring in vivo. Additionally, this technique identified protease auto‐cleavage peptides by their altered mass isotopomer distribution due to incomplete 18O exchange, and modified peptides containing ‘protein carbonyls’ by partial 18O exchange, allowing these peptides to be differentiated during data analysis. Copyright © 2005 John Wiley & Sons, Ltd.