bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding the conditions included in data-driven patterns of multimorbidity: a scoping review
Authors: Sukumaran Luxsena , Winston Alan , Sabin Caroline A 
Published: October 2023

Abstract

Background
Despite the growing utilization of data-driven methods to investigate multimorbidity patterns, there is currently no consensus or guidance on the conditions to include when identifying patterns. This scoping review aims to systematically examine the nature of conditions included in existing studies using data-driven techniques.

Methods
A comprehensive search of three electronic databases (MEDLINE, Web of Science and Scopus) was conducted to identify relevant publications from inception to 28 February 2022 using predefined search terms and inclusion/exclusion criteria. The reference lists and citations of relevant papers were also searched.

Results
Among 7326 search results, 5444 relevant articles were identified. After screening against the eligibility criteria, 60 articles were included in the review. Half of the reviewed studies reported selection criteria for conditions, with prevalence in the population of interest being the most common criterion (40%). Most studies included at least one neurological [59 (98.3%)], musculoskeletal [58 (96.7%)], respiratory [57 (95.0%)] or mental health [56 (93.3%)] condition. In contrast, only a small proportion of studies included skin [17 (28.3%)], infections [14 (23.3%)] or autoimmune conditions [10 (16.7%)]. Nine conditions (hypertension, diabetes, cancer, arthritis, COPD, asthma, depression, stroke and osteoporosis) were included by more than half of the studies.

Conclusions
This review highlights the considerable heterogeneity among the conditions included in analyses of multimorbidity patterns. Researchers should provide a clear rationale for the selection of conditions to facilitate comparisons across studies and ensure reproducibility, as well as consider selecting a diverse range of conditions to capture the complexity of multimorbidity.
Effect of gum tragacanth–sodium alginate coatings incorporated with epigallocatechin gallate on the quality and shelf life of large yellow croaker (Larimichthys crocea) during superchilling storage
Authors: Xu Zhilong , Pei Juxin , Mei Jun , Yu Huijie , Chu Siyuan , Xie Jing 
Published: October 2023

Abstract
The effect of gum tragacanth (GT) and sodium alginate (SA) active coatings incorporated with epigallocatechin gallate (EGCG; 0.16%, 0.32%, and 0.64%, respectively) on the quality of large yellow croaker fillets was evaluated during superchilling storage at –3 ˚C for 42 d. The results revealed that the GT-SA-EGCG active coatings delayed microbial spoilage by reducing the total viable counts of Pseudomonas spp. and psychrophile bacteria and retarded the degradation of the physical properties of water-holding capacity, cooking loss, color, odor and texture. GT-SA-EGCG coatings could also maintain fillet freshness by maintaining lower total volatile basic nitrogen, K value, and histidine content, inhibiting myofibrillar protein degradation during superchilling storage. These results suggested that the active coatings embedded with GT-SA-EGCG could be used as a preservative to enhance the quality of large yellow croaker and prolong the shelf life by 7–14 d during superchilling storage at –3 ˚C.
Inhibitory effect of bilobalide on Staphylococcus aureus von Willebrand factor-binding protein and its therapeutic effect in mice with pneumonia
Authors: Hou Juan , Jiang Yijing , Xu Yangming , Zhao Chunhui , Cao Yali , Song Wu , Wang Bingmei 
Published: October 2023

Abstract

Aims
Disabling bacterial virulence with small molecules has been proposed as a potential strategy to prevent bacterial pathogenicity. The von Willebrand factor-binding protein of Staphylococcus aureus was identified previously as a key virulence determinant. Our objective was to discover a von Willebrand-factor binding protein (vWbp) inhibitor distinct from the antibiotics used to prevent infections resulting from S. aureus.

Methods and results
Using coagulation assays, we found that the sesquiterpene trilactone bilobalide blocks coagulation mediated by vWbp, but has no impact on the growth of S. aureus at a concentration of 128 μg ml−1. Moreover, a mouse model of pneumonia caused by S. aureus indicated that bilobalide could attenuate S. aureus virulence in vivo. This effect is achieved not by interfering with the expression of vWbp but by binding to vWbp, as demonstrated by western blotting, thermal shift assays, and fluorescence quenching assays. Using molecular dynamic simulations and point mutagenesis analysis, we identified that the Q17A and R453A residues are key residues for the binding of bilobalide to vWbp.

Conclusions
Overall, we tested the ability of bilobalide to inhibit S. aureus infections by targeting vWbp and explored the potential mechanism of this activity.
The effect of bone and analytical methods on the assessment of bone mineralization response to dietary phosphorus, phytase, and vitamin D in nursery pigs
Authors: Williams Hadley R , Chin Taylor E , Tokach Mike D , Woodworth Jason C , DeRouchey Joel M , Goodband Robert D , Bergstrom Jon R , Rahe Michael C , Siepker Christopher L , Sitthicharoenchai Panchan , Radke Scott L , Ensley Steve M , Gebhardt Jordan T 
Published: October 2023

AbstractA total of 360 pigs (DNA 600 × 241, DNA; initially 11.9 ± 0.56 kg) were used in a 28-d trial to evaluate the effects of different bones and analytical methods on the assessment of bone mineralization response to dietary P, vitamin D, and phytase in nursery pigs. Pens of pigs (six pigs per pen) were randomized to six dietary treatments in a randomized complete block design with 10 pens per treatment. Dietary treatments were designed to create differences in bone mineralization and included: (1) 0.19% standardized total tract digestibility (STTD) P (deficient), (2) 0.33% STTD P (NRC [2012] requirement) using monocalcium phosphate, (3) 0.33% STTD P including 0.14% release from phytase (Ronozyme HiPhos 2700, DSM Nutritional Products, Parsippany, NJ), (4) 0.44% STTD P using monocalcium phosphate, phytase, and no vitamin D, (5) diet 4 with vitamin D (1,653 IU/kg), and (6) diet 5 with an additional 50 µg/kg of 25(OH)D3 (HyD, DSM Nutritional Products, Parsippany, NJ) estimated to provide an additional 2,000 IU/kg of vitamin D3. After 28 d on feed, eight pigs per treatment were euthanized for bone (metacarpal, 2nd rib, 10th rib, and fibula), blood, and urine analysis. The response to treatment for bone density and ash was dependent upon the bone analyzed (treatment × bone interaction for bone density, P = 0.044; non-defatted bone ash, P = 0.060; defatted bone ash, P = 0.068). Thus, the response related to dietary treatment differed depending on which bone (metacarpal, fibula, 2nd rib, or 10th rib) was measured. Pigs fed 0.19% STTD P had decreased (P &lt; 0.05) bone density and ash (non-defatted and defatted) for all bones compared to 0.44% STTD P, with 0.33% STTD P generally intermediate or similar to 0.44% STTD P. Pigs fed 0.44% STTD P with no vitamin D had greater (P &lt; 0.05) non-defatted fibula ash compared to all treatments other than 0.44% STTD P with added 25(OH)D3. Pigs fed diets with 0.44% STTD P had greater (P &lt; 0.05) defatted second rib ash compared to pigs fed 0.19% STTD P or 0.33% STTD P with no phytase. In summary, bone density and ash responses varied depending on bone analyzed. Differences in bone density and ash in response to P and vitamin D were most apparent with fibulas and second ribs. There were apparent differences in the bone ash percentage between defatted and non-defatted bone. However, differences between the treatments remain consistent regardless of the analytic procedure. For histopathology, 10th ribs were more sensitive than 2nd ribs or fibulas for the detection of lesions.
Operative Innovation and Surgical Conservatism in Twentieth-Century Ulcer Surgery
Authors: Crenner Christopher 
Published: October 2023

Abstract
Peptic ulcers were a common, and seemingly intractable, problem for surgeons in the US through the early twentieth century. Initial surgical efforts reduced operative mortality and achieved short term successes but failed to establish a definitive solution. The flawed successes of early ulcer surgery drove sustained effort to improve, producing a stream of novel operations over the decades. An examination of the history of ulcer surgery confirms the recent observation that surgical operations of this period were malleable entities subject to constant tinkering and repurposing. Yet, this dynamism in surgical practice remained in tension with conservative pressures, as surgeons hung on to familiar practices and sought to codify agreement on which operation served best for which purpose. Ulcer surgery became a workshop for attempts to resolve this tension. In this context, a canon of recognized operations emerged that accommodated novelties while preserving in surgical discourse an awareness of older operations. Established operations that fell from use literally remained on the books for decades. This compromise between innovation and operative conservatism favored the creative reuse of older ulcer operations, some repurposed, and some combined with other operations in new modular configurations. Ulcer surgery demonstrated recurring patterns of operative repurposing, reconfiguration, and modular recombination. This feature of twentieth-century ulcer surgery also highlights the attachment in modern surgical culture to the historicity of their endeavor, manifested for example in the wide use of eponyms and a fondness for deep genealogies of mentoring and training.
Amoxicillin treatment of pneumococcal pneumonia impacts bone marrow neutrophil maturation and function
Authors: Mondemé Mélanie , Zeroual Yasmine , Soulard Daphnée , Hennart Benjamin , Beury Delphine , Saliou Jean-Michel , Carnoy Christophe , Sirard Jean-Claude , Faveeuw Christelle 
Published: October 2023

Abstract
Pneumonia caused by Streptococcus pneumoniae is a leading cause of death worldwide. A growing body of evidence indicates that the successful treatment of bacterial infections results from synergy between antibiotic-mediated direct antibacterial activity and the host's immune defenses. However, the mechanisms underlying the protective immune responses induced by amoxicillin, a β-lactam antibiotic used as the first-line treatment of S. pneumoniae infections, have not been characterized. A better understanding of amoxicillin's effects on host-pathogen interactions might facilitate the development of other treatment options. Given the crucial role of neutrophils in the control of S. pneumoniae infections, we decided to investigate amoxicillin's impact on neutrophil development in a mouse model of pneumococcal superinfection. A single therapeutic dose of amoxicillin almost completely eradicated the bacteria and prevented local and systemic inflammatory responses. Interestingly, in this context, amoxicillin treatment did not impair the emergency granulopoiesis triggered in the bone marrow by S. pneumoniae. Importantly, treatment of pneumonia with amoxicillin was associated with a greater mature neutrophil count in the bone marrow; these neutrophils had specific transcriptomic and proteomic profiles. Furthermore, amoxicillin-conditioned, mature neutrophils in the bone marrow had a less activated phenotype and might be rapidly mobilized in peripheral tissues in response to systemic inflammation. Thus, by revealing a novel effect of amoxicillin on the development and functions of bone marrow neutrophils during S. pneumoniae pneumonia, our findings provide new insights into the impact of amoxicillin treatment on host immune responses.
Prevalence of pelvic pain in transgender individuals on testosterone
Authors: Grimstad Frances W , Boskey Elizabeth R , Clark Rachael S , Ferrando Cecile A 
Published: October 2023

Abstract

Background
Pelvic pain has been reported in transmasculine individuals taking testosterone. There is a need for further investigation to increase understanding of the prevalence and risk factors of this pain.

Aim
We sought to determine the prevalence of pelvic pain reported by transmasculine individuals who had both a uterus and ovaries and were taking testosterone.

Methods
We conducted an institutional review board–approved retrospective study of all transmasculine individuals who had been taking testosterone for at least 1 year and had a uterus and ovaries at the time of testosterone initiation. Charts of participating patients were reviewed to determine patient characteristics, testosterone use, and pelvic pain symptoms both before and after initiation of testosterone.

Outcomes
Patients reported experiences of pelvic pain while on testosterone.

Results
Of 280 individuals who had been on testosterone for at least 1 year, 100 (36%) experienced pelvic pain while on testosterone. Of those patients, 71% (n = 71) had not experienced pelvic pain prior to starting testosterone. There were 42 patients (15%) who had pelvic pain prior to starting testosterone, 13 (31%) of whom no longer experienced pain once starting testosterone. The median (IQR) age at initiation of testosterone was 22 (19-41) years and duration of testosterone treatment was 48 (27-251) months.
Those patients who experienced pelvic pain while on testosterone were significantly more likely to have also reported pelvic pain prior to starting testosterone (29% vs 7%, P &lt; .001). These patients were also more likely to have a pre-existing diagnosis of dysmenorrhea (27% vs 7%, P &lt; .001), endometriosis (6% vs 2%, P = .049), or ovarian cysts and/or masses (12% vs 2% P &lt; .001). Patients with pelvic pain were also more likely to have been on a menstrual suppression agent prior to and overlapping testosterone initiation (22% vs 12%, P = .03) and to have used menstrual suppression for longer durations (median [IQR] 18 [6-44] vs 8 [4-15] months, P = .04).

Clinical Implications
Pelvic pain is common in transmasculine individuals who are initiating testosterone treatment, although testosterone has both positive and negative effects on pelvic pain in different individuals.

Strengths and Limitations
The major strengths of this study included large numbers of patients, ability to assess for documentation of pelvic pain prior to testosterone, and ability to determine an actual prevalence of pelvic pain. Major limitations included the study being a retrospective analysis in a single tertiary care center, the limitations of clinical documentation, and the lack of a standard pelvic pain evaluation process.

Conclusion
More than one-third of transmasculine patients with a uterus and ovaries had pelvic pain while on testosterone, with the majority reporting onset of pain after initiating testosterone.
Erskine Nicol and the representation of national and religious identities in nineteenth-century Ireland
Authors: NicGhabhann Niamh 
Published: October 2023

Abstract
This article investigates the visual representation of Irish Catholicism in mid-nineteenth century genre painting through a close analysis of St Patrick’s Day (1856), a picturesque genre painting by the Scottish artist Erskine Nicol. In doing so, it will explore why artists like Nicol, Frederic William Burton and others chose to equate Irish rural Catholicism with romantic ideas of nature and outdoor ‘primitive’ worship. This tradition of representation will be examined in the context of contemporary Catholic institutional expansion across Ireland following the removal of the legal disabilities known as the ‘penal laws’ in 1829, and the parallel emphasis on the church building as the location of worship, the sacraments, and Catholic devotional life.
To date, scholars of Nicol’s oeuvre have tended to focus on Nicol’s representation of an Irish national ‘type’ or ‘character’. My reading of Nicol’s St Patrick’s Day, however, aims to extend the art historical investigation of the signification of the Irish stereotype in Victorian painting, and to examine Nicol’s painting within the interwoven frameworks of national identity and religious identity. This analysis of St Patrick’s Day is rooted in a consideration of both genre and historical context, facilitating new insights into ideas of ‘peasant’ or ‘primitive’ Catholicism as central to mid-Victorian representations of the Irish ‘national character’.
The gain-of-function mutation blf13 in the barley orthologue of the rice growth regulator NARROW LEAF1 is associated with increased leaf width
Authors: Jöst Moritz , Soltani Ouad , Kappel Christian , Janiak Agnieszka , Chmielewska Beata , Szurman-Zubrzycka Miriam , McKim Sarah M , Lenhard Michael 
Published: October 2023

Abstract
Canopy architecture in cereals plays an important role in determining yield. Leaf width represents one key aspect of this canopy architecture. However, our understanding of leaf width control in cereals remains incomplete. Classical mutagenesis studies in barely identified multiple morphological mutants, including those with differing leaf widths. Of these, we characterized the broad leaf13 (blf13) mutant in detail. Mutant plants form wider leaves due to increased post-initiation growth and cell proliferation. The mutant phenotype perfectly co-segregated with a missense mutation in the HvHNT1 gene which affected a highly conserved region of the encoded protein, orthologous to the rice NARROW LEAF1 (NAL1) protein. Causality of this mutation for the blf13 phenotype is further supported by correlative transcriptomic analyses and protein–protein interaction studies showing that the mutant HvNHT1 protein interacts more strongly with a known interactor than wild-type HvHNT1. The mutant HvHNT1 protein also showed stronger homodimerization compared with wild-type HvHNT1, and homology modelling suggested an additional interaction site between HvHNT1 monomers due to the blf13 mutation. Thus, the blf13 mutation parallels known gain-of-function NAL1 alleles in rice that increase leaf width and grain yield, suggesting that the blf13 mutation may have a similar agronomic potential in barley.
Efficacy of a membrane concentration method combined with real-time PCR for detection of Giardia and Cryptosporidium in drinking water
Authors: Jingyi Jiang , Ping Yao , Jian Xu , Jia Chen , Xujian Mao , Qiong Li , Bowen Tu , Fengming Wang 
Published: October 2023

Abstract
The occurrence of Giardia and Cryptosporidium (oo)cysts in drinking source water poses a serious public health risk. Here, we established a method that combines membrane concentration and real-time polymerase chain reaction (PCR) to quantify Giardia and Cryptosporidium in drinking water. The water samples were filtered through a cellulose membrane to collect Giardia and Cryptosporidium, and then nucleic acids were extracted. Specific primers and probes were designed and synthesized according to the gph gene sequence of Giardia and 18S rRNA gene sequence of Cryptosporidium. The concentrations of the two targets were determined using real-time PCR technology. The sensitivity, specificity, and stability of the method were evaluated. Our findings revealed that the detection limits of real-time PCR method for detecting Giardia and Cryptosporidium were 0.926 and 0.65 copy/µL, respectively; the spiked recovery rates were above 60% and 38%, respectively, and relative standard deviations were under 0.95% and 2.26%, respectively. Therefore, this effective procedure based on the membrane concentration method and real-time PCR will be useful for detecting Giardia and Cryptosporidium in drinking water for purpose of continuous environmental monitoring.
Constraining the FRB mechanism from scintillation in the host galaxy
Authors: Kumar Pawan , Beniamini Paz , Gupta Om , Cordes James M 
Published: October 2023

ABSTRACT
Most fast radio burst (FRB) models can be divided into two groups based on the distance of the radio emission region from the central engine. The first group of models, the so-called ‘nearby’ or magnetospheric models, invoke FRB emission at distances of 109 cm or less from the central engine, while the second ‘far-away’ models involve emission from distances of 1011 cm or greater. The lateral size for the emission region for the former class of models (≲107 cm) is much smaller than the second class of models (≳109 cm). We propose that an interstellar scattering screen in the host galaxy is well-suited to differentiate between the two classes of models, particularly based on the level of modulations in the observed intensity with frequency, in the regime of strong diffractive scintillation. This is because the diffractive length scale for the host galaxy’s interstellar medium scattering screen is expected to lie between the transverse emission-region sizes for the ‘nearby’ and the ‘far-away’ class of models. Determining the strength of flux modulation caused by scintillation (scintillation modulation index) across the scintillation bandwidth (∼1/2πδts) would provide a strong constraint on the FRB radiation mechanism when the scatter broadening (δts) is shown to be from the FRB host galaxy. The scaling of the scintillation bandwidth as ∼ν4.4 may make it easier to determine the modulation index at ≳ 1 GHz.
Cosmology from LOFAR Two-metre Sky Survey Data Release 2: angular clustering of radio sources
Authors: Hale C L , Schwarz D J , Best P N , Nakoneczny S J , Alonso D , Bacon D , Böhme L , Bhardwaj N , Bilicki M , Camera S , Heneka C S , Pashapour-Ahmadabadi M , Tiwari P , Zheng J , Duncan K J , Jarvis M J , Kondapally R , Magliocchetti M , Rottgering H J A , Shimwell T W 
Published: October 2023

ABSTRACT
Covering $\sim 5600\, \deg ^2$ to rms sensitivities of ∼70−100 $\mu$Jy beam−1, the LOFAR Two-metre Sky Survey Data Release 2 (LoTSS-DR2) provides the largest low-frequency (∼150 MHz) radio catalogue to date, making it an excellent tool for large-area radio cosmology studies. In this work, we use LoTSS-DR2 sources to investigate the angular two-point correlation function of galaxies within the survey. We discuss systematics in the data and an improved methodology for generating random catalogues, compared to that used for LoTSS-DR1, before presenting the angular clustering for ∼900 000 sources ≥1.5 mJy and a peak signal-to-noise ≥ 7.5 across ∼80 per cent of the observed area. Using the clustering, we infer the bias assuming two evolutionary models. When fitting angular scales of $0.5 \le \theta \lt 5{^\circ }$, using a linear bias model, we find LoTSS-DR2 sources are biased tracers of the underlying matter, with a bias of $b_{\rm C}= 2.14^{+0.22}_{-0.20}$ (assuming constant bias) and $b_{\rm E}(z=0)= 1.79^{+0.15}_{-0.14}$ (for an evolving model, inversely proportional to the growth factor), corresponding to $b_{\rm E}= 2.81^{+0.24}_{-0.22}$ at the median redshift of our sample, assuming the LoTSS Deep Fields redshift distribution is representative of our data. This reduces to $b_{\rm C}= 2.02^{+0.17}_{-0.16}$ and $b_{\rm E}(z=0)= 1.67^{+0.12}_{-0.12}$ when allowing preferential redshift distributions from the Deep Fields to model our data. Whilst the clustering amplitude is slightly lower than LoTSS-DR1 (≥2 mJy), our study benefits from larger samples and improved redshift estimates.