bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrastructural and functional changes during metamorphosis of a proleg muscle and its innervation in Galleria mellonella (L.) (Lepidoptera: Pyralididae)
Authors: Randall Winnifred C. , Pipa Rudolph L. 
Published: February 2005

AbstractStructural and functional changes have been correlated during metamorphic degeneration of a single muscle fiber, the plantar retractor of G. mellonella, its axon, and their junctions to determine which features persist as long as muscle contractility. Changes commence simultaneously in muscle and nerve near cuticular attachments, and spread towards the center. Alterations associated with the muscle, including appearance of collapsed tracheoles and mitochondria with dense bodies, begin late in the last larval instar. Within 12 hours after pupal ecdysis some tracheolar withdrawal occurs, sarcoplasmic reticulum becomes reduced, and many mitochondria have dense bodies, dense membranes, or are enlarged. By 17–19 hours primary myofilaments and striations begin to disappear, microtubules and autophagic vacuole‐like bodies appear, and phagocytes invade the muscle. It remains partially contractile upon electrically stimulating its nerve, the ventral nerve, until these changes spread throughout the fiber.Neuromuscular junction changes, including appearance of dense mitochondria and isolation bodies, begin late in the last larval instar. Junctions become fewer, and none remain in those muscle areas where tracheoles, sarcoplasmic reticulum, and primary myofilaments have disappeared.Preliminary studies on nerve discharge activity to the muscle suggest that nerve silence occurs at approximately the time when the muscle loses all contractility. In some axons isolation bodies appear and neurotubules are lost, other axons remain unchanged, and new ones develop later in the pupal state. Phagocytes invade the neural lamella and it disappears in the late pupa, but it reappears in the adult.The adult ventral nerve has over three times more axons and a thinner layer of glial cells than the larval nerve.
Mechanics of respiration in the snapping turtle, Chelydra serpentina (Linné)
Authors: Gaunt Abbot S. , Gans Carl 
Published: February 2005

AbstractThe mechanism of lung ventilation in chelonians has been much debated. Electromyographic studies show that the basic mechanism in the snapping turtle, Chelydra serpentina, is dependent on the activities of four major respiratory muscles that are capable of varying the volume of the visceral cavity. The precise mechanism utilized varies in response to environmental factors, especially the depth to which the animal is submerged. Chelydra tends to reduce muscular activity to a minimum, and hydrostatic pressure or gravity replaces muscular effort whenever possible. The response is subject to hysteresis. Both the mechanics and pattern of ventilation in Chelydra differ from those of Testudo. The differences appear to be attributable in part to Chelydra's markedly reduced plastron and more extensive respiratory musculature and in part to the different habitats occupied by the two species.
Functional components versus mechanical units in descriptive morphology
Authors: Gans Carl 
Published: February 2005

AbstractThe concept of functional components was initially proposed by van der Klaauw ('45, '52) to indicate overlap of functional influences particularly in mammalian skulls; his analysis marked a departure from the study of single characters to that of function‐modified systems. A very similar set of terms is now coming into vogue to describe the mechanically separable components of highly kinetic fish, amphibian and reptilian skulls. In these cases the term functional unit often pertains only to the musculo‐skeletal system and is utilized during the process of description; it is often applied before a complete functional analysis has been carried out.Yet, any structure tends to be affected by the influence of multiple functions, and any function will almost certainly affect multiple characteristics of the animal. Since functional components overlap, the term should not be used to label an essentially topographical dissection of the animal. It cannot be expected that each loosely connected component of a kinetic skull subserves as a single “function,” and that this function does not overlap onto other units.It is suggested that the term mechanical unit be substituted as a label for the mechanical sub‐divisions often utilized to organize descriptions. The concept of functional units in the original sense then remains available as an analytical tool.
A comparative study of the adrenergic innervation of the iris and ciliary structures in 18 species in phylogenesis
Authors: Staflova Jaroslava 
Published: February 2005

AbstractA detailed topography of adrenergic innervation in invertebrates (lobster), low vertebrates (fish, amphibians, reptiles, birds), and nine species of mammals is presented. Flack and Hillarp's specific fluorescent histochemical method using freeze‐dried material was used. Phylogenetically, adrenergic innervation appeared earlier under the ciliary epithelium and in the muscle than surrounding the vessels, and in all species many fibers were without any connection to the vessel walls. Adrenergic innervation was very rich in the dilator muscle extending toward the epithelium of the posterior chamber; a surprisingly rich network was found in the sphincter muscle and also in ciliary spaces of some species. Numerous fluorescent mast cells were visualized in the pecten of the bird eye and in the ciliary tissue of the sheep and cow.
Evidence for the fusion of myoblasts in amphibian embryos. I. Homoplastic transplantations of somitic material labeled with tritiated thymidine
Authors: Loeffler Clarence A. 
Published: February 2005

AbstractIn order to obtain more direct evidence for the occurrence of myoblast fusion in the developing amphibian embryo, the following transplantations were performed in vitro. The nuclei of early embryos. Ambystoma tigrinum and A. maculatum, were labeled with tritiated thymidine. Portions of prospective somite areas from these labeled donors were grafted homoplastically and orthotopically into unlabeled hosts of the same, or nearly the same, stage. The stages employed were: neurula, early tail bud, and late tail bud. Hosts were raised until they had developed into more advanced larval forms, fixed, sectioned, and prepared for radioautographic processing according to the customary procedures.The histological preparations contained varying numbers of multinucleate myotubes of a “composite” nature: that is, individual myotubes contained labeled nuclei of the donor, side by side with unlabeled nuclei of the host. There was no doubt that the mononucleate myoblasts of the grafts had fused with those of the host species to form the mutlinucleate composite myotubes.In addition to the above determinations, the method of thymidine labeling has proven to be a satisfactory method of tracing, in the context of the intact organism, somitic cell derivatives up to the feeding larval stage. Mesenchymal cells from the grafted labeled somitic tissues were consequently found in: dermatomic, sclerotomic and intermyotomic locations; the matrix of the dorsal fin; the limb bud; the abdominal muscles.
The ultrastructure of the cornea‐lens epidermis in the lateral eye of Limulus polyphemus
Authors: Whitehead Russell A. , Purple Richard L. , Hopper Kenneth 
Published: February 2005

AbstractThe structure of the “Corneagen,” i.e., the epidermis lying beneath the cornea‐lens of the lateral eyes of the adult intermolt Limulus polyphemus was studied with light and electron microscopy.This layer is composed of heavily columnar cells containing a striking number of cytoplasmic microtubules. Many of the microtubules are grouped into compact bundles or fascicles, generally each cell having at least one microtubule bundle. The cornealens end of each cell has numerous microvilli, each with a core of delicate filaments. The crypts between microvilli end in extracellular expansions and plaques of electron dense amorphous material are associated with these terminal expansions. Cytoplasmic microtubules appear to insert into these dense areas.The basal ends of the cells are thrown into many pseudopodial processes which extend into the surrounding extracellular space. The cytoplasm of the pseudopodia is composed largely of microtubules and their associated low density halos.Junctional complexes consisting of zonulae adhaerens and septate desmosomes are present between adjacent cells. Mitochondria, ER, cytoplasmic vesicles, Golgi stacks and other ultrastructural details of the epidermal cells are described. The ultrastructure of a column of pigment free processes lying between the apex of the lens cone and the underlying photoreceptive portion of the ommatidium is also described. Ducts or vessels of uncertain origin are present in the inter‐ommatidial spaces.Possible roles played by the microtubules, the significance of their disposition and of their association with the dense subsurface plaques are discussed in terms of intracellular support, epidermis‐lens attachment and extracellular pattern determination. In addition, the likelihood of the dense plaques being the site of microtubule assembly is considered.
A comparative study of the ring glands from wild type and 1(2)gl mutant Drosophila melanogaster
Authors: Aggarwal Surinder K. , King Robert C. 
Published: February 2005

AbstractDrosophila melanogaster homozygous for the second chromosomal, recessive lethal gene 1(2)gl form puparia much later than wild type (+) and are unable to metamorphose. Implantation of + ring glands accelerates puparium formation by 1(2)gl hosts. The ring gland is a compound structure containing the prothoracic glands (pg), corpus allatum (ca), and corpus cardiacum (cc). An electron microscopic study demonstrates that in both the pg and ca the most common subcellular component is smooth surfaced endoplasmic reticulum (ser). In + flies the amount of ser per pg increases by ten times during the four hour prepupal period. In 1(2)gl flies which have spent two days in the prepupal period the pg looks juvenile and contains only 1% the amount of ser per cell found in the + prepupal pg. The ca cells look alike in both + and 1(2)gl individuals, and the cortical cells of the cc in both contain abundant neurosecretory spheres. We suggest that the + allele of 1(2)gl indirectly influences the synthesis of ser by the pg and that this is the site where dietary cholesterol is transformed to ecdysone.
Genesis of the reproductive system of mosquitoes. I. Female of Aedes stimulans (Walker)
Authors: Ronquillo Maria C. , Horsfall William R. 
Published: February 2005

AbstractThe feminine dimorph has unique structures that produce eggs, select salubrious sites for the offspring, store sperm, and void the eggs. This paper provides a time table for development of these parts in Aedes stimulans based on preparations examined at 5‐hour intervals when reared at 21°C. All growths of imaginal parts proceeds independent of activities in the larval tissues.Ovaries produce the eggs in terminal follicles of the ovarioles. Besides ovarioles each ovary contains sheaths for the ovarioles, pedicels attaching them to a central canal, the calyx, ovarian sheath and muscles. Ovaries are recognizable in newly hatched larvae as caps of cells on larger masses which become part of the delivery system for eggs. Each ovary grows forward from its attachment first as a column of cells that differentiates into the several tissues by the time the insect enters pupal life. Prior accounts have considered the ovary as the whole mass of cells on each side of the hemocoel of segment 6. Only the most anterior cells recognizably distinct at the end of embryogeny are generative.The delivery system for eggs is composed of the lateral oviducts and median or common oviduct. Primordia from which the former are derived are present from the end of embryogeny and throughout larval life as two distinct parts. Two ovoid masses occur in the hemocoel of segment 6. To each of these is attached a filament extending backward to an attachment ventrally and caudally in segment 7. They are rapidly changed into definitive lateral oviducts late in pupal life. The single primordium for generating the median genital tract appears during instar 3 as a caudal ventral plate of cells in segment 8 between a pair of bilateral buds and invaginates during instar 4 to form (1) the common oviduct from a midventral pouch, (2) three spermathecae from two lateral invaginations and (3) the elaborate vaginal area. The bilateral buds form no parts of the female.The post‐vaginal area or atrium with its accessory organs is derived in part from the ventral plate of segment 8 and that of segment 9. The imaginal disc in segment 9 is present at the end of embryogeny as primordial buds and ventral plate and development is delayed until early pupal life when it projects inward to form part of the atrium and pouches once to form the common opening for the duct of the accessory gland and the canal to the bursa copulatrix. The buds of this disc produce no feminine parts.During the second larval instar lateral primordia appear as a pair of shields in the anal segment. They develop slowly until pupation when they extend caudally as two flaps called “cerci” in culicid literature and this paper.
Differentiation and degeneration in the motor horn of the foetal mouse
Authors: Flanagan Ann Elizabeth Harris 
Published: February 2005

AbstractThe development of the motor horn of the foetal mouse was investigated. A sampling technique was evolved using cresyl violet stained material.A decline in the number of motor neuroblasts occurred during the development, on each side, of four definitive motor regions from a single longitudinal column. The total number of motor horn cells fell from about 100,000 on the eleventh day after mating to about 25,000 on the fourteenth day. The early stages of this decline (between 11 and 12 days) are probably explained by the fact that not all neuroblasts in the region differentiate into motor cells; he later decrease can be entirely accounted for by the number of degenerations.Irradiation of the foetal mouse with a dose of 50 rads of cobalt‐60 gamma‐radiation resulted in an excess of about 20,000 differentiating motor cells on the thirteenth and fourteenth days. Irradiation increased the actual number, but not the percentage, of degenerations. This suggests that differentiation is a phase in the growth process which proceeds degeneation.
The hatching thread of praying mantids: An unusual chintinous structure
Authors: Kenchington W. 
Published: February 2005

AbstractThe hatching threads of praying mantis embryos are silk‐like in appearance, but cellular in origin. Their development can be divided into four phases. In phase 1 each embryonic cercus produces a hollow column of cells which is pushed out dorso‐laterally, on either side of the embryo, between the epidermis and the chorion. In phase 2 each column becomes transformed into an unbroken helical cellular filament. The terminal five or six cells at the distal end of each filament become permanently attached to the inner surface of the chorion. The cellular arrangement of the filament is superseded by an apparent syncytial condition. In phase 3 the extensive proximal parts of the two filaments become folded into a compact space, flanked by the cerci and styli, at the tip of the abdomen. Throughout phase 4 the filaments remain in two tightly coiled groups, connected to the chorionic attachments by loosely coiled distal regions. Progressive secretion of chitin by, and around, each filament forms a sheath, 1–2 μ thick, which provides the tensile component of the hatching threads. During emergence these threads become unravelled to form a double thread which allows the insect to hang from the ootheca and complete its first ecdysis.
Studies on the nature and localization of acid phosphatase in normal and lens‐regenerating urodele eyes. II. Biochemical assay
Authors: Miller Neil R. , Rafferty Nancy S. 
Published: February 2005

AbstractBiochemical assay of acid phosphatase in normal and lens‐regenerating eyes of the urodele Diemictylus viridescens, using p‐nitrophenyl phosphate as substrate, demonstrates both soluble and lysosomal fractions of the enzyme. While the specific activity of the soluble fraction remains unchanged during lens regeneration, the lysosomal fraction shows four distinct rises in specific activity during the thirty‐day regeneration period studied. These peak activities on the second, eighth, fifteenth, and twenty‐second days post‐lentectomy apparently correspond to lysosomal activity in the processes of wound healing, iris depigmentation, and lens differentiation which occur during urodele lens regeneration. On the basis of biochemical and histochemical studies as well as observations of morphological changes in the urodele eye as lens regeneration proceeds, it is postulated that there is a significant correlation between these morphological changes and the level and localization of the lysosomal acid hydrolases in the tissues in which the changes occur.
A comparative study of the embryological development of the median cord in hemiptera
Authors: Springer Charles Anthony , Rutschky Charles W. 
Published: February 2005

AbstractMedian cord development is uniform in six families of Hemiptera and five non‐hemipterans. The median cord arises independently from the lateral cords and is histologically distinguishable from the latter throughout development. Intrasegmentally, median cord nuclei possess prominent nucleoli and many small chromatin granules surrounded by clear nuclear sap. This region forms what appear to be glial elements at the midline of the neuropile. Intersegmentally, a spherical clump of eight to twelve large nuclei develops surrounded by dark‐staining granular cytoplasm. Each intersegmental clump migrates anteriorly into the preceding ganglionic region but degenerates soon after katatrepsis.
Morphology of the trunk of Corynosoma hamanni (acanthocephala: Polymorphidae)
Authors: Holloway Harry L. , Nickol Brent B. 
Published: February 2005

AbstractMorphological study of Corynosoma hamanni (Linstow, 1892) was continued with the intention of presenting a morphological description of a single, well defined species.Sexual dimorphism is exhibited in body size, males being larger, and in body spination. Trunk spines are arranged in a pattern similar to that of Corynosoma semerme and do not extend onto the bursa as reported in the original description of C. hamanni.Two testes, located side by side, are followed by six cement glands arranged in two groups of three glands each. One group is behind each testis. Cement ducts from glands on the right side cross over Saefftigen's pouch and join to form a reservoir on the left side; ducts from cement glands on the left side fuse forming a second reservoir. The two cement reservoirs unite before emptying into the ejaculatory duct. The bursa possesses rays on its outer wall and numerous, well‐developed papillae in the muscular cap.In females, a ruptured genital ligament sac is attached partially on the dorsal wall of the uterine bell and partially on a column of six cells projecting from the bell's base into its lumen. Postericrly the dorsal wall of the bell has two protruding pockets behind which occur ten cells composing the selector apparatus. Dorsal openings into the pseudocoelom occur on either side of the uterine bell. Two muscular tubes conduct eggs into the uterus. Female C. hamanni frequently form genital vestibules in which the genital pore occurs along the dorsal wall and not, as reported for some species, at the anterior tip.Eggs of C. hamanni possess four membranes rather than the previously reported three.
Seasonal changes in testicular histology of three species of the lizard genus Uma
Authors: Mayhew Wilbur W. , Wright Shirley J. 
Published: February 2005

AbstractDesert‐dwelling male Uma notata, U. inornata and U. scoparia were autopsied monthly from 1959 through 1962. Seasonal changes in the histological morphology of the testes were observed. A series of eight stages, indicating these changes in spermatogenic activity, was established. These categories arbitrarily divide a continuous process into several recognizable stages.Present evidence indicates that male breeding cycles can be determined rather precisely by examining any of several reproductive features. Data from several sources reveal that the male breeding season of U. scoparia is shorter than that of U. notata or U. inornata. Nevertheless, the testes of all three species appear to function reproductively during the hottest month of the year. Possible explanations for this ability are suggested.Little difference was observed in the time the seminiferous tubules of U. notata testes reached full breeding condition (stage 6) following dry winters. This is in contrast to the time motile sperm appeared at the cloacal end of the vas deferens in these animals. Thus is appears that the major cause of postponement in male U. notata reproductive activity following dry winters is due to a delay in sperm reaching the vas deferens rather than a retardation in sperm production.
The spleen of the cowbird
Authors: Ewart David F. , McMillan Donald B. 
Published: February 2005

AbstractA histological study of the spleen of the Brown‐headed cowbird, Molothrus ater, is presented.One of the most striking differences from the mammalian spleen is the lack of trabeculae and of smooth muscle in the capsule which would suggest that the spleen is not an organ of storage or pumping of blood. Without trabeculae to foster the close association of the major arteries and veins, these vessels take separate courses. Their support is provided by elaboration of the collagenous and reticular fibres of the stroma.A peculiar ovoid structure, the ampulla, carries the blood from the terminal arterioles of the white pulp to both the sinusoids and the reticular cords of the red pulp so that both open and closed circulations are seen but the open circulation predominates. The ampulla has perforated walls consisting of a simple cuboidal endothelium surrounded by a dense reticular sleeve. Leucocytes were seen passing through the holes in the walls of the ampullae by diapedesis. It is suggested that the ampullae may be contractile and act as sphincters controlling the flow of blood through the spleen.The major functions of the spleen appear to be haemopoiesis, production of antibodies, and filtration of blood.
Ultrastructure of human labial salivary glands. III. Myoepithelium and ducts
Authors: Tandler Bernard , Denning Carolyn R. , Mandel Irwin D. , Kutscher Austin H. 
Published: February 2005

AbstractMyoepithelial cells were present between the basal lamina and the acinar secretory cells of human labial salivary glands. In form and disposition, they resembled myoepithelial cells in the major salivary glands. Many of these cells possessed single cilia on their upper surfaces. Such cilia occasionally extended into invaginations of the overlying secretory cell.The intercalated ducts were variable in occurrence. Their epithelium ranged from columnar to squamous, and showed few signs of secretory activity.Few intralobular ducts possessed basal striations. While mitochondria were abundant in non‐striated cells, they were randomly disposed in both basal and apical cytoplasm, and the basal plasmalemma showed only occasional infoldings. The paucity of true striated ducts in labial salivary glands may be responsible for the high concentration of sodium and chloride in unstimulated labial gland salivary secretions.