bluuebunny/crossref_metadata_2025_split huggingface dataset:
TAXONOMIC REVISION OF THE CONJUGATOPHYCEAN FAMILY PENIACEAE ON THE BASIS OF CELL WALL ULTRASTRUCTURE1
Authors: C.Kouwets Frans A. , M.Coesel Peter F. 
Published: November 2004

ABSTRACTAn ultrastructural investigation of the cell wall of Penium silvae‐nigrae Raban. and P. spinospermum Josh. showed that these species possess true pores with a pore apparatus and overlapping semi‐cell walls. It follows that these two taxa belong not to the Peniaceae, but to the Desmidiaceae sensu stricto; they are referred to the genus Actinotaenium Teil. on account of the shape of their cells and chloroplasts. Two other species previously included in Penium Bréb. are referred to Actinotaenium. Although their cell wall structure could not be studied, they are distinguished from “typical” representatives of Penium by the following photomicroscopically observable complex of features: (pseudo‐) girdle bands none, cell wall pores in longitudinal rows, zygospores not globose but of irregular shape. The following new combinations ensued: Actinotaenium borgeanum (Skuja), A. phymatosporum (Nordst.), A. silvae‐nigrae (Raban.), A. silvaenigrae var. parallelum (Krieger) and A. spinospermum (Josh.). In addition the diagnosis of the genus Penium was emended and P. margaritaceum (Ehr.) ex Bréb. was selected as the lectotype species. The family Gonatozy‐gaceae is merged into the Peniaceae on the basis of cell wall structure.
STRUCTURE AND SIGNIFICANCE OF THE CRYPTOMONAD FLAGELLAR APPARATUS. I.CRYPTOMONAS OVATA(CRYPTOPHYTA)1
Authors: Roberts Keith R. 
Published: November 2004

ABSTRACTThe absolute configuration of the flagellar apparatus inCryptomonas ovatahas been elucidated and found to be similar to that reported forChilomonas paramecium.Variations apparent in the flagellar apparatus ofCryptomonas ovatainclude the presence of striations in the mitochondrion associated lamella, a rhizostyle which does not bear wing‐like extensions from the microtubules and does not lie close to the nucleus, and a striated fibrous anchoring structure associated with one basal body which has not hitherto been described. The flagellar apparatus also includes a four stranded microtubular root which traverses into the anterior dorsal lobe of the cell, a striated fibrous root which is associated with a five stranded microtubular root, and a two stranded Cr root. The homologous nature of these roots to those in the larger cryptomonads is discussed in relation to the apparent reduction in flagellar apparatus size and complexity among the smaller cryptomonads. A diagrammatic reconstruction of the flagellar apparatus ofCryptomonas ovatais also presented.
The CCR5Δ32 allele is associated with reduced liver inflammation in hepatitis C virus infection
Authors: Wald O. , Pappo O. , Ari Z. B. , Azzaria E. , Wiess I. D. , Gafnovitch I. , Wald H. , Spengler U. , Galun E. , Peled A. 
Published: November 2004

SummaryCCR5Δ32 is a deletion mutation in the chemokine receptor CCR5. Liver inflammatory activity was found to be significantly reduced (P = 0.005) in Jewish Israeli patients infected with the hepatitis C virus (HCV) carrying the CCR5Δ32 allele. The CCR5Δ32 allele does not alter susceptibility to HCV infection; however, it may play a role in the progression and outcome of the disease.
Establishment of T cell‐specific and natural killer cell‐specific unigene sets: towards high‐throughput genomics of leukaemia
Authors: Illiger J. , Herwig R. , Steinfath M. , Przewieslik T. , Elge T. , Bull Ch. , Radelof U. , Lehrach H. , Janitz M. 
Published: November 2004

SummaryWe report the establishment of highly non‐redundant unigene sets consisting of cDNA clones derived from T lymphocytes and natural killer cells. Each set consists of 10 506 and 13 409 clones, respectively, arrayed on nylon membranes in duplicate. The sets provide an excellent tool for genome‐wide gene expression analysis studies in immunology research.
Gypsy moth feeding in the canopy of a CO2‐enriched mature forest
Authors: Hättenschwiler Stephan , Schafellner Christa 
Published: November 2004

AbstractRising atmospheric carbon dioxide (CO2) concentration is expected to change plant tissue quality with important implications for plant–insect interactions. Taking advantage of canopy access by a crane and long‐term CO2 enrichment (530 μ mol mol−1) of a natural old‐growth forest (web‐free air carbon dioxide enrichment), we studied the responses of a generalist insect herbivore feeding in the canopy of tall trees. We found that relative growth rates (RGR) of gypsy moth (Lymantria dispar) were reduced by 30% in larvae fed on high CO2‐exposed Quercus petraea, but increased by 29% when fed on high CO2‐grown Carpinus betulus compared with control trees at ambient CO2 (370 μ mol mol−1). In Fagus sylvatica, there was a nonsignificant trend for reduced RGR under elevated CO2. Tree species‐specific changes in starch to nitrogen ratio, water, and the concentrations of proteins, condensed and hydrolyzable tannins in response to elevated CO2 were identified to correlate with altered RGR of gypsy moth larvae. Our data suggest that rising atmospheric CO2 will have strong species‐specific effects on leaf chemical composition of canopy trees in natural forests leading to contrasting responses of herbivores such as those reported here. A future change in host tree preference seems likely with far‐ranging consequences for forest community dynamics.
Advantages of Different Tillage Systems and Their Effects on the Economically Important Pests, Thrips tabaci Lind. and Aphis gossypii Glov. in Cotton Fields
Authors: Gencsoylu I. , Yalcın I. 
Published: November 2004

AbstractAn experiment to assess advantages of different tillage and their effects on Aphis gossypii Glov. and Thrips tabaci Lind was conducted during the 2000 and 2001 cotton growing seasons in cotton fields at Adnan Menderes University, Agricultural Faculty Research Center, located in Aydın Province, Turkey. The tillage systems examined were conventional, strip, precision and ridge tillage. Two insect population densities were not affected by the type of tillage systems during either year. However, significant differences in populations were observed in thrips populations on 31 May 2000 and 18 May 2001 and aphid populations on 24 May 2000 and 11 May 2001. The results show that the highest yield was observed in all conservation tillage systems in 2000 and ridge tillage system in 2001. Early maturity was higher in both precision and ridge tillage systems. In addition, tillage systems did not affect fibre properties. As a result, the application of conservation tillage is more advantageous in cotton in respect of early maturity and total yield.
Impact of Soil Tillage and Crop Rotation on Barley (Hordeum vulgare) and Weeds in a Semi‐arid Environment
Authors: Ghosheh H. Z. , Al‐Hajaj N. A. 
Published: November 2004

AbstractExperiments were conducted to evaluate the effect of mouldboard‐ or chisel‐ploughing and rotations on barley crops and associated weeds in a semi‐arid location. Two primary soil tillage operations and eight crop rotation‐tillage operation combinations were evaluated over two successive seasons. Drought conditions prevailed (<152 mm annual precipitation) and affected the measured parameters. Barley grown in mouldboard‐ploughed plots had higher biomass compared with chisel‐ploughed plots. Barley grain yield was greater in mouldboard‐ploughed plots in a fallow‐fallow‐barley rotation. Weed species densities varied between tillage systems and rotations. Density of Hordeum marinum, for example, was high in fallow‐barley‐fallow in chisel‐ploughed plots, and was high under more continuous fallow in mouldboard‐ploughed plots. Similar variations were also observed in weed fresh weights and in numbers of seed produced. The results describe the productivity of barley under extremely dry conditions, where an advantage for mouldboard ploughing was observed. The results also indicate the complexity of weed communities in their response towards different tillage‐rotation combinations.
New microsatellite primers for Daphnia galeata mendotae
Authors: FOX JENNIFER A. 
Published: November 2004

AbstractIn order to study genetic changes in populations of Daphnia galeata mendotae, I characterized seven polymorphic microsatellite loci. Primers to amplify these loci were tested on individual eggs from the resting egg bank of Onondaga Lake, NY. Levels of polymorphism and cross‐amplification in D. g. galeata indicate that they will be useful markers for ecological genetic studies on both adults and diapausing eggs of these species.
Ten species specific microsatellite loci for Lahontan cutthroat trout, Oncorhynchus clarki henshawi
Authors: PEACOCK MARY M. , NEVILLE HELEN , KIRCHOFF VERONICA S. 
Published: November 2004

AbstractTen polymorphic microsatellite loci (containing tri and tetra‐nucleotide repeats) were developed for the Lahontan cutthroat trout (Oncorhynchus clarki henshawi), a subspecies of cutthroat trout listed as threatened under the United States Endangered Species Act. Polymorphism was assessed for 445 individuals from 12 populations representing eight watersheds spread throughout the three Distinct Population Segments defined for this subspecies. All loci were polymorphic (X̄ = 19, range 7–26 alleles). All loci were in Hardy–Weinberg equilibrium (HWE) except for one locus (OCH 9) in a single population (P < 0.00014 after Bonferroni correction for multiple tests).
Polymorphic microsatellite DNA markers from the Oriental Fruit Fly Bactrocera dorsalis (Hendel)
Authors: DAI S. M. , LIN C. C. , CHANG C. 
Published: November 2004

AbstractSix polymorphic microsatellite loci are isolated from the Oriental fruit fly Bactrocera dorsalis (Hendel), an agricultural pest in Asia, including Taiwan. To assess their potential utility as high‐resolution genetic markers, polymerase chain reaction (PCR) primers, amplification conditions, and an automated fluorescence detection protocol were developed. In analyses of 71 individual flies from six different areas of Taiwan, allele numbers ranged from five to 25 were detected for each locus. The observed heterozygosity ranged between 0.268 and 0.737 among these loci. No linkage disequilibrium was found. These microsatellite markers have potential utility to population structure and gene flow studies of B. dorsalis (Hendel).
Characterization of microsatellite loci in Primula modesta Bisset et Moore (Primulaceae)
Authors: SHIMONO A. , UENO S. , TSUMURA Y. , WASHITANI I. 
Published: November 2004

AbstractEleven microsatellite loci were isolated from alpine plant Primula modesta. Enriched repeat libraries were constructed and screened by colony hybridization. Forty‐eight polymerase chain reaction (PCR) primer pairs were designed, of which 11 pairs producted successful amplification. A total of 31–35 adult individuals were genotyped for allelic diversity. The polymorphism ranged from three to 14 alleles, and the heterozygosity ranged from 0.161 to 0.828. No linkage disequilibrium was found between these 11 loci.
Isolation of five polymorphic microsatellite markers in Utetheisa ornatrix (Lepidoptera: Arctiidae)
Authors: BEZZERIDES ALEXANDER , BEZZERIDES JULIE , EISNER THOMAS 
Published: November 2004

AbstractWe isolated five polymorphic microsatellite markers for the arctiid moth Utetheisa ornatrix as part of an effort to investigate the role of inbreeding in the degree of polyandry seen in natural populations of the moth. We tested the primers on 105 individuals collected from scrub habitat in central Florida. These microsatellite loci provide a new research tool for understanding the population structure and reproductive behaviour of U. ornatrix.
A new set of microsatellite markers for the peach palm (Bactris gasipaesKunth); characterization and across‐taxa utility within the tribe Cocoeae
Authors: BILLOTTE N. , COUVREUR T. , MARSEILLAC N. , BROTTIER P. , PERTHUIS B. , VALLEJO M. , NOYER J.‐L. , JACQUEMOUD‐COLLET J.‐P. , RISTERUCCI A.‐M. , PINTAUD J.‐C. 
Published: November 2004

AbstractA (GA)nmicrosatellite‐enriched library was constructed and a new set of 18 nuclear simple sequence repeat loci was isolated inBactris gasipaesvar.gasipaes. The loci were found to be highly variable in the target species and readily transferable to relatedBactrisspecies as well as to theAstrocaryumandElaeisgenera of the same Cocoeae tribe. These microsatellite resources are made available to study the genetic diversity and gene flow within theBactriscomplex for a better understanding of the domestication process of the peach palm and for further Cocoeae genetics.
Isolation and characterization of five microsatellite loci in the ponerine ant Pachycondyla inversa (Hymenoptera, Formicidae)
Authors: TRINDL A. , HEINZE J. , D’ETTORRE P. 
Published: November 2004

AbstractWe characterized five polymorphic DNA microsatellite loci for the neotropical ponerine ant Pachycondyla inversa. The variability was initially tested in 19 workers from nine colonies from a Brazilian population. We found five to 12 alleles per locus, with observed heterozygosities between 0.72 and 0.95. The allele size ranged from 73 to 197 bp. The primers also successfully amplified DNA at all five loci in the closely related species P. villosa.
Isolation and characterization of six polymorphic microsatellite loci in the western flower thrips Frankliniella occidentalis (Insecta, Thysanoptera)
Authors: BRUNNER P. C. , FREY J. E. 
Published: November 2004

AbstractHighly polymorphic microsatellite markers can provide important demographic information on founder events and range expansion following initial introduction of invasive insect species. Six microsatellite loci were isolated from an enriched DNA library in order to study the invasion patterns of the western flower thrips, Frankliniella occidentalis. All loci tested were found to be polymorphic and successfully amplified in all individuals. The number of alleles per locus ranged from five to nine and heterozygosity ranged from 45 to 73%. Some of the loci were also successfully amplified in other thrips species.
Characterization of microsatellite loci in Kearney's bluestar (Amsonia kearneyana) and cross‐amplification in other Amsonia species
Authors: TOPINKA J. RICK , DONOVAN ARTHUR J. , MAY BERNIE 
Published: November 2004

AbstractKearney's bluestar (Amsonia kearneyana) is a highly endangered herbaceous perennial in the family Apocynaceae. The species is found only in the Baboquivari Mountains of southern Arizona. We report the isolation and development of 12 microsatellite loci for Kearney's bluestar. Numbers of alleles ranged from two to four and observed heterozygosities ranged from 0.20 to 0.80 in the nine loci found to be polymorphic in the test population. All loci were also tested for cross‐amplification in five other Amsonia species representing two subgenera from the southwestern United States. Some loci that were not polymorphic in the Kearney's bluestar were polymorphic in other species.
A set of microsatellite markers for use in Sitka spruce (Picea sitchensis) developed from Picea glauca ESTs
Authors: A'HARA S. W. , COTTRELL J. E. 
Published: November 2004

AbstractFew microsatellite markers have been specifically developed for Picea sitchensis. In January 2004 the appearance of over 10 000 sequences of expressed regions of DNA from P. glauca in GenBank presented an opportunity for the development of additional microsatellite markers in Sitka spruce. Mono‐ and dinucleotide repeat sequences were located in these sequences and primers were designed around these regions. Amplification was attempted in Sitka material from a broad geographical range and the level of polymorphism was assessed. Primers were also tested in progeny of a controlled cross. Nine polymorphic loci that demonstrated Mendelian inheritance in Sitka were discovered in this study.
Primers for 12 polymorphic microsatellite DNA loci from the guppy (Poecilia reticulata)
Authors: OLENDORF ROBERT , REUDI BETH , HUGHES KIMBERLY A. 
Published: November 2004

AbstractTwelve polymorphic microsatellite DNA loci were isolated from genomic guppy DNA. We assessed the level of genetic diversity for these loci using individuals from five native Trinidadian populations. All the loci were polymorphic although there were considerable differences among loci and populations in allele frequencies. Within populations, the number of alleles ranged from 1 to 22 and observed heterozygosities ranged from 0.000 to 0.990. Allele frequencies differed substantially between populations suggesting divergence.
matesoft: a program for deducing parental genotypes and estimating mating system statistics in haplodiploid species
Authors: MOILANEN ATTE , SUNDSTRÖM LISELOTTE , PEDERSEN JES SØE 
Published: November 2004

AbstractWith the advent of sophisticated genetic markers, studies on mating systems and reproductive apportionment have become increasingly feasible. In particular, paternity analyses in haplodiploid species have gained in power as hemizygous paternal genotypes allow maternal and paternal genotypes to be directly inferred from offspring genotypes. The computer program matesoft offers both newly developed algorithms for inferring maternal and paternal genotypes, and integrated estimation and correction procedures for calculating mating frequency statistics. Standard data are offspring genotypes of male‐haplodiploid organisms, including social Hymenoptera.
Characterization of microsatellite loci in Sacramento perch (Archoplites interruptus)
Authors: SCHWARTZ RACHEL S. , MAY BERNIE 
Published: November 2004

AbstractWe characterized 23 polymorphic tetranucleotide microsatellite loci for Sacramento perch (Archoplites interruptus). This species is extirpated in its native range, the Sacramento–San Joaquin Delta (California, USA), and is therefore targeted for recovery. A concerted effort is currently underway to re‐establish self‐sustaining populations of Sacramento perch in its native range. These microsatellites will be used to analyse the population structure of the species and, in conjunction with life history and physiological data, develop a comprehensive recovery plan.
Polymorphic microsatellite markers from the formicine ant Lasius (Dendrolasius) fuliginosus
Authors: FALK KARL H. , LIEBIG JÜRGEN , GADAU JÜRGEN 
Published: November 2004

AbstractSix polymorphic microsatellite loci were isolated and characterized from the temporary social parasitic ant Lasius fuliginosus by a highly efficient enrichment procedure. Observed allele numbers ranged from three to 20 per locus, whereby four out of the six tested loci had more than 10 alleles and showed both observed and expected heterozygosities greater than 70%. For each locus we present suitable primer sequences. With these microsatellite markers we will be able to reveal colony and population structure of L. fuliginosus.
genecap: a program for analysis of multilocus genotype data for non‐invasive sampling and capture‐recapture population estimation
Authors: WILBERG MICHAEL J. , DREHER BRIAN P. 
Published: November 2004

AbstractWe createdgenecapto facilitate analysis of multilocus genotype data for use in non‐invasive DNA sampling and genetic capture‐recapture studies.genecapis a Microsoftexcelmacro that uses multilocus genetic data to match samples with identical genotypes, calculate frequency of alleles, identify sample genotypes that differ by one and two alleles, calculate probabilities of identity, and match probabilities for matching samples.genecapallows the user to include background data and samples with missing genotypes for multiple loci. Capture histories for each user‐defined sampling period are output in formats consistent with commonly employed population estimation programs.
Skin tensile properties revisited during ageing. Where now, where next?
Authors: Hermanns‐Lê Trinh , Uhoda Isabelle , Smitz Simon , Piérard Gérald E 
Published: November 2004

SummarySkin and its subcutaneous layer represent a complex composite of tissues, whose mechanical characteristics depend upon the mutual interdependence of their constituent parts. The molecular and microanatomical structures of skin allow it to meet normal mechanical demands. They also determine the orientation both of Langer's lines and of relaxed skin tension lines. Ageing, photodamage, hormones, drugs, cosmetic products and dermatological interventions may modify the skin's overall tensile properties. In turn, any variation in mechanical stresses and strains imposed upon the skin's connective tissue influences the metabolic activity and phenotypic expression of fibroblasts and dermal dendrocytes.The viscoelastic functions of ageing skin can be tested by altering the orientation and magnitude of imposed stresses and strains over time. Assessment can be made of various biomechanical properties of skin: tensile, torsional, acoustic shear wave, indentation, impact and elevation. Such objective biomechanical assessments may be applied to dermocosmetic interventions, so providing opportunity for progress in cosmetic dermatological science.
Interventions to support reflection and learning: a qualitative study
Authors: Holmström Inger , Rosenqvist Urban 
Published: November 2004

AbstractThe aim of this research was to explore the role of a professional development supervisor in helping health professionals to reflect on their role in patient consultations. This article is written from the viewpoint of patient‐centred care and seeks to examine how the mentor/supervisor can facilitate reflection and learning by discussing, with professionals, videotapes of their regular meetings with patients. The specific context of the article is an educational intervention in Sweden, based on modern theories of competence development for professionals in diabetes care. A purposeful sample of 18 patients with type 2 diabetes agreed to have their regular meeting with a general practitioner (GP) or diabetes nurse videotaped. The GPs or diabetes nurses of these patients then participated in an intervention, comprising sessions when they reviewed with a supervisor their consultations with the patients. These supervising sessions were videotaped and are investigated in this article. The supervisor's role was to make observations that could alter the professional's understanding of the diabetes consultation through video‐cued narrative reflection. Five overarching themes were identified from the content (the what‐aspect) of the intervention: medical problems; patient characteristics and reactions; patient education; the consultation as such; and the healthcare organization. The activities used in carrying out the intervention (the how‐aspect) were: confirmation and feedback; interpretation; reflective questioning; suggestions and corrections; and closed‐end questions or brief answers. It was hypothesized that this way of conducting reflective educational interventions would be beneficial for healthcare professionals when working with patients with various longstanding medical conditions, recognizing that a patient‐centred approach which included the patient's learning and possibilities for self‐management are more suitable for the chronic illness encounter.