bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis
Authors: Liu Ying , Murazzi Ileana , Fuller Ashley M. , Pan Hehai , Irizarry-Negron Valerie M. , Devine Ann , Katti Rohan , Skuli Nicolas , Ciotti Gabrielle E. , Pak Koreana , Pack Michael A. , Simon M. Celeste , Weber Kristy , Cooper Kumarasen , Eisinger-Mathason T.S. Karin 
Published: February 2024

AbstractIntratumoral hypoxia correlates with metastasis and poor survival in patients with sarcoma. Using an impedance sensing assay and a zebrafish intravital microinjection model, we demonstrated here that the hypoxia-inducible collagen-modifying enzyme lysyl hydroxylase PLOD2 and its substrate collagen type VI (COLVI) weaken the lung endothelial barrier and promote transendothelial migration. Mechanistically, hypoxia-induced PLOD2 in sarcoma cells modified COLVI, which was then secreted into the vasculature. Upon reaching the apical surface of lung endothelial cells, modified COLVI from tumor cells activated integrin β1 (ITGβ1). Furthermore, activated ITGβ1 colocalized with Kindlin2, initiating their interaction with F-actin and prompting its polymerization. Polymerized F-actin disrupted endothelial adherens junctions and induced barrier dysfunction. Consistently, modified and secreted COLVI was required for the late stages of lung metastasis in vivo. Analysis of patient gene expression and survival data from The Cancer Genome Atlas (TCGA) revealed an association between the expression of both PLOD2 and COLVI and patient survival. Furthermore, high levels of COLVI were detected in surgically resected sarcoma metastases from patient lungs and in the blood of tumor-bearing mice. Together, these data identify a mechanism of sarcoma lung metastasis, revealing opportunities for therapeutic intervention.Significance:Collagen type VI modified by hypoxia-induced PLOD2 is secreted by sarcoma cells and binds to integrin β1 on endothelial cells to induce barrier dysfunction, which promotes sarcoma vascular dissemination and metastasis.
Single-Cell Analysis Identifies NOTCH3-Mediated Interactions between Stromal Cells That Promote Microenvironment Remodeling and Invasion in Lung Adenocarcinoma
Authors: Xiang Handan , Pan Yidan , Sze Marc A. , Wlodarska Marta , Li Ling , van de Mark Karyn Ann , Qamar Haleema , Moure Casey J. , Linn Douglas E. , Hai Josephine , Huo Ying , Clarke James , Tan Tze Guan , Ho Samantha , Teng Karen W. , Ramli Muhammad N. , Nebozhyn Michael , Zhang Chunsheng , Barlow Julianne , Gustafson Corinne E. , Gornisiewicz Savanna , Albertson Thomas P. , Korle Stephanie L. , Bueno Raphael , Moy Lily Y. , Vollmann Elisabeth H. , Chiang Derek Y. , Brandish Philip E. , Loboda Andrey 
Published: February 2024

Abstract

Cancer immunotherapy has revolutionized the treatment of lung adenocarcinoma (LUAD); however, a significant proportion of patients do not respond. Recent transcriptomic studies to understand determinants of immunotherapy response have pinpointed stromal-mediated resistance mechanisms. To gain a better understanding of stromal biology at the cellular and molecular level in LUAD, we performed single-cell RNA sequencing of 256,379 cells, including 13,857 mesenchymal cells, from 9 treatment-naïve patients. Among the mesenchymal cell subsets, FAP+PDPN+ cancer-associated fibroblasts (CAF) and ACTA2+MCAM+ pericytes were enriched in tumors and differentiated from lung-resident fibroblasts. Imaging mass cytometry revealed that both subsets were topographically adjacent to the perivascular niche and had close spatial interactions with endothelial cells (EC). Modeling of ligand and receptor interactomes between mesenchymal and ECs identified that NOTCH signaling drives these cell-to-cell interactions in tumors, with pericytes and CAFs as the signal receivers and arterial and PLVAPhigh immature neovascular ECs as the signal senders. Either pharmacologically blocking NOTCH signaling or genetically depleting NOTCH3 levels in mesenchymal cells significantly reduced collagen production and suppressed cell invasion. Bulk RNA sequencing data demonstrated that NOTCH3 expression correlated with poor survival in stroma-rich patients and that a T cell–inflamed gene signature only predicted survival in patients with low NOTCH3. Collectively, this study provides valuable insights into the role of NOTCH3 in regulating tumor stroma biology, warranting further studies to elucidate the clinical implications of targeting NOTCH3 signaling.

Significance:
NOTCH3 signaling activates tumor-associated mesenchymal cells, increases collagen production, and augments cell invasion in lung adenocarcinoma, suggesting its critical role in remodeling tumor stroma.
Adding New Dimensions to 3D Cancer Models
Authors: Chu Kevan , Dow Lukas E. 
Published: February 2024

Abstract
Understanding patient-specific responses to anticancer therapies and how individual tumors interact with their tumor microenvironment (TME) is a challenging task. To measure the impact of the TME on diverse and clinically relevant treatments, Ramos Zapatero and colleagues coupled patient-derived organoid (PDO) and cancer-associated fibroblast (CAF) cocultures with high-throughput mass cytometry–based assessment of cell state. Using a newly developed “Trellis” algorithm enabled integration and analysis of highly complex, multidimensional treatment response data. This work showed that tumor cell response to chemotherapy was associated with both intrinsic and nonintrinsic signaling states, whereby proliferative rate, growth factor signaling, and CAFs interaction influenced chemoprotection. Furthermore, the work suggests a potential role for the TME in promoting lineage plasticity associated with drug resistance. In all, the pipeline described provides a blueprint for exploring the intricate interplay of factors influencing cancer treatment response.
Association of HIV and HCV Infection With Carotid Artery Plaque Echomorphology in the MACS/WIHS Combined Cohort Study
Authors: Bravo Claudio A. , Moon Jee-Young , Davy Krista , Kaplan Robert C. , Anastos Kathryn , Rodriguez Carlos J. , Post Wendy S. , Gange Stephen J. , Kassaye Seble G. , Kingsley Lawrence A. , Lazar Jason M. , Mack Wendy J. , Pyslar Nataliya , Tien Phyllis C. , Witt Mallory D. , Palella Frank J. , Li Yanjie , Yan Mingzhu , Hodis Howard N. , Hanna David B. 
Published: February 2024

BACKGROUND:
HIV and hepatitis C virus (HCV) are associated with increased risk of carotid artery atherosclerotic plaque and stroke. We examined associations of HIV- and HCV-related factors with echomorphologic features of carotid artery plaque.

METHODS:
This cross-sectional study included participants from the MACS (Multicenter AIDS Cohort Study)/WIHS (Women’s Interagency HIV Study) Combined Cohort Study who underwent high-resolution B-mode carotid artery ultrasound. Plaques were characterized from 6 areas of the right carotid artery. Poisson regression controlling for demographic and cardiometabolic risk factors determined adjusted prevalence ratios (aPRs) and 95% CIs for associations of HIV- and HCV-related factors with echomorphologic features.

RESULTS:

Of 2655 participants (65% women, median age 44 [interquartile range, 37–50] years), 1845 (70%) were living with HIV, 600 (23%) were living with HCV, and 425 (16%) had carotid plaque. There were 191 plaques identified in 129 (11%) women with HIV, 51 plaques in 32 (7%) women without HIV, 248 plaques in 171 (28%) men with HIV, and 139 plaques in 93 (29%) men without HIV. Adjusted analyses showed that people with HIV and current CD4
+
count <200 cells/µL had a significantly higher prevalence of predominantly echolucent plaque (aPR, 1.86 [95% CI, 1.08–3.21]) than those without HIV. HCV infection alone (aPR, 1.86 [95% CI, 1.08–3.19]) and HIV-HCV coinfection (aPR, 1.75 [95% CI, 1.10–2.78]) were each associated with higher prevalence of predominantly echogenic plaque. HIV-HCV coinfection was also associated with higher prevalence of smooth surface plaque (aPR, 2.75 [95% CI, 1.03–7.32]) compared with people without HIV and HCV.

CONCLUSIONS:
HIV with poor immunologic control, as well as HCV infection, either alone or in the presence of HIV, were associated with different echomorphologic phenotypes of carotid artery plaque.
Interim Report on Gribshunden (1495) Excavations: 2019–2021
Authors: Foley Brendan 
Published: February 2024

Abstract
We present an interim archaeological report on the shipwreck of the Danish-Norwegian flagship Gribshunden. Built in 1485, extensively employed by King Hans as his floating castle, and ultimately lost in June 1495 en route to Kalmar, the ship carried people and material selected to further the political goal of re-unifying the Nordic region. Three field campaigns led by Lund University and Blekinge Museum after 2019 have delivered a compelling assemblage of mundane artefacts and high-status items, some without precedent, and all conveying new information beyond the events of the ship’s loss. Highlights from these efforts and the 2019 excavation include wooden casks, a milled tankard, a purse of silver coins, artillery and accoutrements, several crossbows and accessories, mail armour elements, and an assortment of exotic spices. Laboratory analyses of these and other finds allow new avenues of inquiry and provide deep insights into the late medieval world. This manuscript is derived from a conference presentation. It delivers an overview of recent scientific results, alerts readers to detailed studies already published, and offers waypoints to ongoing research threads.
The Impact of Martin Luther’s Religious Reform on the Conversion of New Chinese Immigrants to Christianity
Authors: Zeng Weifu 
Published: February 2024

Abstract
Through a study of the M Church in Bangkok, this research paper explores the influence of Martin Luther’s Reformation spirit of freedom of religion on the conversion of new Chinese immigrants to Christianity. With the increase in the number of Chinese who have emigrated overseas since the reform and opening up, Christianity has become increasingly popular among new Chinese immigrants, and the concept of freedom of religion advocated by Martin Luther’s Reformation has played an active role in this process. This study first, introduces the background of Martin Luther’s Reformation and his advocacy of religious freedom. Then, the literature related to Martin Luther’s Reformation, the Christian faith of new immigrants in China, and the Christian faith of Chinese in Thailand is reviewed. Then, through field research, the history and current situation of the new Chinese immigrants’ participation in the M Church in Bangkok are analyzed, and the transmutation of their beliefs in this chapel is discussed. Through interviews and surveys, the paper further analyzes the reasons for the conversion of Chinese new immigrants to Christianity. The study shows that the concept of religious freedom advocated by Martin Luther’s Reformation has played a positive role in the conversion of Chinese new immigrants to Christianity. However, Chinese new immigrants face many difficulties and challenges in the process of converting to Christianity, such as understanding and identifying with the faith and understanding the cultural differences between China and the West. Therefore, this paper argues that the concept of religious freedom advocated by Martin Luther’s Reformation has played an important role in the conversion of new Chinese immigrants to Christianity, but this process also needs to be combined with the integration and development of traditional Chinese culture.
A Mixed Methods Analysis of Standardized Documentation of Serious Illness Conversations Within an Electronic Health Record Module During Hospitalization
Authors: Serna Myrna Katalina , Yoon Catherine , Fiskio Julie , Lakin Joshua R. , Schnipper Jeffrey L. , Dalal Anuj K. 
Published: February 2024

Background Analysis of documented Serious Illness Conversations (SICs) in the inpatient setting can help clinicians align management to address patient and caregiver needs. Methods We conducted a mixed methods analysis of the first instance of standardized documentation of a SIC within a structured module among hospitalized general medicine patients from 2018 to 2019. Percentage of documentations that included a description of patient or family understanding of the patient’s medical condition and use of radio buttons to answer the “prognostic information shared,” “hopes,” and “worries” modules are reported. Using grounded theory approach, physicians analyzed free text entries to: “What is important to the patient/family?” and “Recommendations or next steps planned.” Results Out of 5142 patients, 59 patients had a documented SIC. Patient or family understanding of the medical condition(s) was reported in 56 (95%). For “prognostic information shared,” the most frequently selected radio buttons were: 49 (83%) incurable disease and 28 (48%) prognosis of weeks to months while those for “hopes” were: 52 (88%) be comfortable and 27 (46%) be at home and for “worries” were: 49 (83%) other physical suffering and 36 (61%) pain. Themes generated from entries to “What’s important to patient/family?” included being with loved ones; comfort; mentally and physically present; and reliable care while those for “Recommendations” were coordinating support services; symptom management; and support and communication. Conclusions SIC content indicated concern about pain and reliable care suggesting the complex, intensive nature of caring for seriously ill patients and the need to consider SICs earlier in the life course of patients.
Effects of Scarcity-Induced Demand on Crowdfunding Market
Authors: Zhou Zhijin , Liu Ye , Ma Chaoliang , Tan Yong 
Published: February 2024

Fundraisers on reward-based crowdfunding platforms have increasingly sought to base their promotional strategy on the principle of scarcity. Although previous studies find that using a limited-edition inventory contributes to the success of campaigns, it remains unknown how fundraisers should leverage a scarcity strategy in the upfront design of reward options. Using a unique dynamic panel dataset from a leading reward-based crowdfunding platform, we develop a hierarchical Bayesian model to capture the dynamic arrival process of backers at the reward option level. We find that backers are driven by excess demand rather than limited supply. Putting quantity restrictions on a reward option can discourage demand if it fails to accumulate an adequate number of backers first. In addition, the scarcity strategy is more effective in high-priced reward options than in low-priced reward options. This study offers practical implications for the implementation of a scarcity strategy for both platform managers and fundraisers.
It’s Showtime: Live-Streaming E-commerce and Optimal Promotion Insertion Policy
Authors: Huang Wenpo , Jiang Wei , Luo Xinggang , Mei Xiaowei , Wei Lai 
Published: February 2024

Live-streaming e-commerce has gained tremendous success as a new form of business model over the past few years. Nevertheless, there has been scarce research examining the effect of exogenous stimulus-driven factors (i.e., social cues such as promotion coupons) on the profit of the live-streaming platform. We model the continuous evolvement of the aggregate viewer involvement level with geometric Brownian motion and investigate the optimal timing and depth of promotion insertion policy. Our findings reveal that the optimal promotion insertion policy for the live-streaming room is a threshold policy that consists of a start-to-promote threshold and an involvement target when the trend of the aggregate involvement level is not large and the promotion cost is not high. Under some scenarios, the promotion planning process is constrained by various business rules, such as the promotion depth being limited by a discrete set (e.g., integral multiples of [Formula: see text]). Although this problem involves more complex constraints, we analytically show that a variation of the abovementioned threshold policy is the optimal strategy among all feasible policies, i.e., when the aggregate viewer involvement drops below a certain level ([Formula: see text]), the live-streaming host promotes with the depth of the least integral multiple of [Formula: see text] to raise the viewer involvement level above [Formula: see text]. Finally, to examine the effectiveness of our proposed policy, we compare it with prevalent industry practices. The result shows that the proposed promotion insertion policy outperforms prevailing ones significantly in generating profit for the live-streaming room.
Factors associated with false lumen changes in patients with superior mesenteric artery dissection
Authors: Mei Junhao , Yuan Yuan , Yan Hui , Zhao Xi , Xue Tongqing , Su Haobo , Jia Zhongzhi 
Published: February 2024

Background: False lumen changes (FLCs) are the main reference for the prognosis judgment and treatment plan selection for type IIa superior mesenteric artery dissection (SMAD). Methods: For this retrospective study, 55 patients with symptomatic type IIa SMAD were included. Computational fluid dynamics (CFD) analysis was used to explore the hemodynamic basis of FLCs. Correlation and multiple linear regression analyses were performed to identify clinical, morphological and hemodynamic factors associated with FLCs. Results: The FLCs of patients with successful conservative treatment ( n = 29) are significantly higher than those with failed conservative treatment ( n = 26) (58.5 ± 21.1% vs 10.9 ± 17.4%, p < 0.0001). Positive correlations were seen between FLCs and the morphological parameters false lumen length (FLL)/dissection entrance length (DEL) and FLL. In terms of hemodynamic parameters, negative correlations were seen between FLCs and time-averaged wall shear stress (TAWSS), vorticity, and high areas of TAWSS and vorticity, whereas positive correlations were seen between FLCs and oscillatory shear index (OSI), relative residence time (RRT), and high areas of OSI and RRT. Multiple linear regression analysis identified symptom duration (odds ratio [OR], 0.93; 95% CI, 0.91–0.96; p < 0.0001), FLL/DEL (OR, 1.30; 95% CI, 1.01–1.67; p = 0.044), and high RRT area (OR, 2.03; 95% CI, 1.48–2.78; p < 0.0001) as predictors of FLCs. Conclusion: The clinical predictor symptom duration, morphological factor FLL/DEL, and the hemodynamic factor high RRT area can serve as predictors of FLCs in patients with symptomatic type IIa SMAD.
The Surprising Effect of Priming on SNOT-22 Results
Authors: Mohammad Ibtisam , Stack Taylor , Norris Meghan , Kim Sulgi , Lamb Meredith , Thorp Brian D. , Klatt-Cromwell Christine , Ebert Charles S. , Kimple Adam J. , Senior Brent A. 
Published: February 2024

Background Priming is a psychological phenomenon where subconscious cues in the environment impact our behavioral responses in certain situations. Well studied in the worlds of business, marketing, and even politics, it is unclear how the priming phenomenon impacts patient perception of their own disease state nor how they report that perception using tools like the Sinonasal Outcomes Test (SNOT-22), used to measure that perception in chronic rhinosinusitis. Objective To determine the impact of positive or negative priming on self-reported patient perception of their chronic rhinosinusitis disease using the SNOT-22 disease-specific quality of life instrument. Methods Single-blind, randomized, prospective cohort pilot study of 206 consecutive adult patients with a clinical diagnosis of chronic rhinosinusitis presenting to a university rhinology clinic. Patients were randomized to receive “positive priming” (103) or “negative priming” (103) by reading a passage about the positive or negative aspects of chronic sinusitis and its treatment respectively. Patients were then asked to fill out the SNOT-22 and results between the two groups were compared. Results The negative priming group had a higher median SNOT-22 score of 49 [IQR = 39] compared to the positive priming groups’ score of 22 [IQR = 27], p < 0.0001), a difference of nearly three times the minimal clinical impactful difference (MCID). This effect was consistent regardless of age or sex of the patient. Subgroup analysis revealed a greater impact when priming was performed by the senior male attending regardless of patient age or sex (p < 0.001), while priming performed by the younger female research fellow had greater impact on older patients (>59 years, p = 0.001) and female patients (p = 0.003). Conclusions Priming impacts how patient's perceive their chronic rhinosinusitis as determined by the SNOT-22. It is imperative that the rhinologist understand this when using this instrument in research applications and in clinical decision-making for patients.
Comprehensive genotyping analysis of single nucleotide polymorphisms responsible for beef marbling in Japanese Black cattle
Authors: Sasazaki Shinji , Kondo Hina , Moriishi Yurika , Kawaguchi Fuki , Oyama Kenji , Mannen Hideyuki 
Published: February 2024

Abstract
Background
Beef marbling is considered a desirable trait in the meat industry. Therefore, understanding the genetic factors that cause marbling is important. Previously, we performed a genome-wide association study to examine genetic factors associated with beef marbling in Japanese Black cattle and identified a candidate region between 10–30 Mbp on chromosome 7. We verified the effect of the SNPs in this region on beef marbling using linkage disequilibrium block analysis. We narrowed down the candidate region to a range of 15.8–16.1 Mbp. In this study, we comprehensively detected all of the SNPs in this region and verified their effects on beef marbling.

Results
Genome resequencing using four animals exhibiting high beef marbling standard (BMS) and four with low BMS revealed a total of 1,846 polymorphisms within the candidate region. Based on the annotation, we selected 13 SNPs exhibiting a moderate impact, as no high-impact SNPs were detected. All of the SNPs represented missense polymorphisms and were located in the following seven genes: RDH8, ANGPTL6, DNMT1, MRPL4, ICAM1, ICAM3, and ICAM5. Finally, we determined the effects of these SNPs on the BMS of a Japanese Black cattle population (n = 529). Analysis of variance revealed that the five SNPs were located in genes encoding the intercellular adhesion molecules (ICAM1, ICAM3, and ICAM5), and showed a highly significant association compared with the remainder (p < 0.01). The lowest p-value was observed for ICAM3_c.739G > A (p = 1.18E-04). Previous studies have suggested that intercellular adhesion molecules (ICAM) may be an upstream factor that regulates adipocyte differentiation. Therefore, considering the polymorphism and putative gene function, we suggest that ICAM1 is potentially responsible for beef marbling. c.470C > G and/or c.994G > A on ICAM1 may be responsible for this quantitative trait locus.

Conclusions
Promising SNP candidates responsible for beef marbling were identified using extensive polymorphism verification in a previously reported QTL region. We aim to elucidate the mechanism of beef marbling in future studies by investigating how these polymorphisms alter protein structure and function.