bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural basis for antibody recognition of the proximal MUC16 ectodomain
Authors: Lee Kwangkook , Perry Kay , Xu Mengyao , Veillard Irva , Kumar Raj , Rao Thapi Dharma , Rueda Bo R , Spriggs David R , Yeku Oladapo O 
Published: February 2024

Abstract
Background
Mucin 16 (MUC16) overexpression is linked with cancer progression, metastasis, and therapy resistance in high grade serous ovarian cancer and other malignancies. The cleavage of MUC16 forms independent bimodular fragments, the shed tandem repeat sequence which circulates as a protein bearing the ovarian cancer biomarker (CA125) and a proximal membrane-bound component which is critical in MUC16 oncogenic behavior. A humanized, high affinity antibody targeting the proximal ectodomain represents a potential therapeutic agent against MUC16 with lower antigenic potential and restricted human tissue expression.

Results
Here, we demonstrate the potential therapeutic versatility of the humanized antibody as a monoclonal antibody, antibody drug conjugate, and chimeric antigen receptor. We report the crystal structures of 4H11-scFv, derived from an antibody specifically targeting the MUC16 C-terminal region, alone and in complex with a 26-amino acid MUC16 segment resolved at 2.36 Å and 2.47 Å resolution, respectively. The scFv forms a robust interaction with an epitope consisting of two consecutive β-turns and a β-hairpin stabilized by 2 hydrogen bonds. The VH-VL interface within the 4H11-scFv is stabilized through an intricate network of 11 hydrogen bonds and a cation-π interaction.

Conclusions
Together, our studies offer insight into antibody-MUC16 ectodomain interaction and advance our ability to design agents with potentially improved therapeutic properties over anti-CA125 moiety antibodies.
The efficacy of virtual reality technology for the postoperative rehabilitation of patients with cervical spondylotic myelopathy (CSM): a study protocol for a randomized controlled trial
Authors: Wu Jiajun , Sun Zhongchuan , Ge Zhichao , Zhang Dong , Xu Jianghan , Zhang Rilin , Liu Xuecheng , Zhao Qing , Sun Hao 
Published: February 2024

Abstract
Background
Patients with cervical spondylosis myelopathy (CSM) may experience severe neurological dysfunction due to untimely spinal cord compression after surgery. These disorders may lead to sensory and motion disorders, causing considerable psychological distress. Recent studies found that virtual reality (VR) technology can be an effective tool for treating spinal cord injuries. Owing to this discovery, we developed an exploratory research project to investigate the impact of this intervention on the postoperative recovery of patients with CSM.

Methods
The purpose of this randomized controlled trial was to evaluate the efficacy of combining VR technology with conventional rehabilitation strategies for the postoperative rehabilitation of patients with CSM. A total of 78 patients will be recruited and randomized to either the conventional rehabilitation group or the group subjected to VR technology combined with conventional rehabilitation strategies. The Japanese Orthopaedic Association (JOA) scale will be the main tool used, and secondary outcomes will be measured via the visual analogue scale (VAS), neck disability index (NDI), and functional MRI (fMRI). The data analysis will identify differences between the intervention and control groups as well as any relationship between the intragroup changes in the functional area of the brain and the subjective scale scores after the intervention.

Discussion
The aim of this trial is to investigate the effect of VR training on the postoperative rehabilitation of patients with CSM after 12 intervention treatments. Positive and negative outcomes will help us better understand the effectiveness of the intervention and its neural impact. If effective, this study could provide new options for the postoperative rehabilitation of patients with CSM.

Trial registration
Chinese Clinical Trial Registry (ChiCTR2300071544). Registered 17 May 2023, https://www.chictr.org.cn/.
Spectrophotometric resolution for quantitative analysis of aspirin and rivaroxaban combination therapy in biological fluids using simple and eco-friendly procedure
Authors: Mohamed Heba M. , Essam Hebatallah M. 
Published: February 2024

AbstractPatients diagnosed with symptomatic peripheral artery disease (PAD) in the lower extremities have a higher likelihood of suffering from major vascular events. Recently, FDA has approved the combination therapy of aspirin (ASP) and rivaroxaban (ROX) to reduce acute limb ischemia and other comorbidities in (PAD) patients. Zero order and ratio absorption spectra were employed in three simple and accurate spectrophotometric techniques (dual wavelength (DW), ratio difference (RD) and derivative ratio (1DD) for concurrent detection and quantification of ASP and ROX in their pure forms, lab synthetic mixtures and in biological fluid. Our approach involves careful parameter optimization, including solvent selection, sample volumes, and instrumental settings, to reduce the analysis environmental impact. The acquired recovery percentages of accuracy were within 98–102% for pure active pharmaceutical ingredients and 90–110% for pharmaceutical formulations and biological determinations. A comprehensive assessment was done to compare the three methods regarding their ease of use, linearity, sensitivity, conditions, and limitations. The specificity of the proposed methods was evaluated by analyzing the lab synthetic mixtures. The suggested spectrophotometric methods were validated in compliance with ICH guidelines to confirm the validity claims. Also, statistical analysis was done to compare the outcomes obtained from the suggested methods with those obtained from the official ones and they agreed with null hypothesis regarding accuracy and precision. Furthermore, a comprehensive assessment of the environmental sustainability of the developed method was carried out using the Analytical Greenness Calculator, AGREE algorithm. The selected drugs can be efficiently, safely and economically analyzed by the suggested methods in pharmaceutical and biological matrices with no pretreatment or preliminary separation steps and thereby increasing their greenness level.
Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses
Authors: Terradas Gerard , Manzano-Alvarez Jaime , Vanalli Chiara , Werling Kristine , Cattadori Isabella M. , Rasgon Jason L. 
Published: February 2024

AbstractBackgroundIncreasing global temperatures and unpredictable climatic extremes have contributed to the spread of vector-borne diseases. The mosquitoAedes aegyptiis the main vector of multiple arboviruses that negatively impact human health, mostly in low socioeconomic areas of the world. Co-circulation and co-infection of these viruses in humans have been increasingly reported; however, how vectors contribute to this alarming trend remains unclear.MethodsHere, we examine single and co-infection of Mayaro virus (D strain,Alphavirus) and dengue virus (serotype 2,Flavivirus) inAe. aegyptiadults and cell lines at two constant temperatures, moderate (27 °C) and hot (32 °C), to quantify vector competence and the effect of temperature on infection, dissemination and transmission, including on the degree of interaction between the two viruses.ResultsBoth viruses were primarily affected by temperature but there was a partial interaction with co-infection. Dengue virus quickly replicates in adult mosquitoes with a tendency for higher titers in co-infected mosquitoes at both temperatures, and mosquito mortality was more severe at higher temperatures in all conditions. For dengue, and to a lesser extent Mayaro, vector competence and vectorial capacity were higher at hotter temperature in co- vs. single infections and was more evident at earlier time points (7 vs. 14 days post infection) for Mayaro. The temperature-dependent phenotype was confirmed in vitro by faster cellular infection and initial replication at higher temperatures for dengue but not for Mayaro virus.ConclusionsOur study suggests that contrasting kinetics of the two viruses could be related to their intrinsic thermal requirements, where alphaviruses thrive better at lower temperatures compared to flaviviruses. However, more studies are necessary to clarify the role of co-infection at different temperature regimes, including under more natural temperature settings.Graphical Abstract
Expression of sex-specific molecular markers by Babesia bovis gametes
Authors: Hussein Hala E. , Johnson Wendell C. , Taus Naomi S. , Ueti Massaro W. 
Published: February 2024

Abstract
Background
Bovine babesiosis caused by Babesia bovis is one of the most important tick-borne diseases of cattle in tropical and subtropical regions. Babesia bovis parasites have a complex lifecycle, including development within the mammalian host and tick vector. In the tick midgut, extracellular Babesia parasites transform into gametes that fuse to form zygotes. To date, little is known about genes and proteins expressed by male gametes.

Methods and results
We developed a method to separate male gametes from in vitro induced B. bovis culture. Separation enabled the validation of sex-specific markers. Collected male gametocytes were observed by Giemsa-stained smear and live-cell fluorescence microscopy. Babesia male gametes were used to confirm sex-specific markers by quantitative real-time PCR. Some genes were found to be male gamete specific genes including pka, hap2, α-tubulin II and znfp2. However, α-tubulin I and ABC transporter, trap2-4 and ccp1-3 genes were found to be upregulated in culture depleted of male gametes (female-enriched). Live immunofluorescence analysis using polyclonal antibodies confirmed surface expression of HAP2 by male and TRAP2-4 by female gametes. These results revealed strong markers to distinguish between B. bovis male and female gametes.

Conclusions
Herein, we describe the identification of sex-specific molecular markers essential for B. bovis sexual reproduction. These tools will enhance our understanding of the biology of sexual stages and, consequently, the development of additional strategies to control bovine babesiosis.

Graphical Abstract
Role of gastric bypass surgery in control of blood sugar in obese uncontrolled type 2 diabetic patients
Authors: El-Shreef Hala Khalf Allah , Taha Osama Yaseen Mostafa , Ahmed abd El Hafeez Heba , Shoka Amal Ibrahim Abd El-Rheem Abo 
Published: February 2024

Abstract
Background and goal
The pandemic of the twenty-first century is diabetes. Both type 2 diabetes mellitus and obesity pose severe problems for public health. Despite significant improvements in diagnosing and managing both conditions, diabetes mellitus remains poorly controlled, and diabetic complications are more common than ever. Internists have discovered over the past 20 years that obese people with type 2 diabetes who have gastric bypass surgery to shed weight have improved glycemic control. Thus, interventional diabetology has a growing significance in patients’ ability to reverse type 2 diabetes mellitus. We want to evaluate the impact of gastric bypass on blood sugar regulation and look for potential causes.

Patients and methods
Between 2018 and 2020, a prospective interventional study was carried out. Ninety patients in total were enrolled in the trial. The two patient groups (A and B) contained 45 obese T2DM patients with a body mass index (BMI) of over 35 kg/m2. Group B received its antidiabetic medications, either oral hypoglycemic or insulin, while Group A underwent gastric bypass surgery. Each patient underwent a comprehensive history review and clinical assessment. Both groups had their HA1c and blood sugar levels measured; group A had their insulin, GLP-1, and HOMA-IR (Homeostasis Model Assessment for Insulin Resistance) levels calculated at time O and one year later.

Results
The demographic differences between the two study groups were negligible. After a one-year follow-up, group A had significantly lower anthropometric measurement data for BMI and waist circumference (cm), lipid profile data for triglycerides, total cholesterol, and LDL levels, and systolic and diastolic blood pressure. Fasting plasma glucose and HbA1c, two metrics of glucose metabolism, significantly decreased in group A. Regarding indicators of glucose metabolism, there was a drop in fasting insulin level and HOMA-IR and an increase in GLP1 level in the gastric bypass group.

Conclusion
As a result of improving all indicators, gastric bypass is an effective treatment for patients with uncontrolled T2DM. Future research that is confirmed is needed.
Association between ACTN3 (R577X), ACE (I/D), BDKRB2 (-9/+9), and AGT (M268T) polymorphisms and performance phenotypes in Brazilian swimmers
Authors: de Albuquerque-Neto Severino Leão , Santos Marcos Antonio Pereira dos , Silvino Valmir Oliveira , Herrera Jose Juan Blanco , Rosa Thiago Santos , Silva Glauber Castelo Branco , Couto Bruno Pena , Ferreira Cirley Pinheiro , Silva Alexandre Sérgio , de Almeida Sandro Soares , de Melo Gislane Ferreira 
Published: February 2024

Abstract
Background
The influence of genetic polymorphisms on athletic performance has been widely explored. This study investigated the interactions between the polymorphisms ACTN3 (R577X), ACE (I/D), BDKRB2 (-9/+9), and AGT (M/T) and their association with endurance and strength phenotypes in Brazilian swimmers.

Methods
123 athletes (aged 20–30 years) and 718 controls participated in the study. The athletes were divided into elite and sub-elite (N = 19 and 104, respectively) and strength and endurance experts (N = 98 and 25, respectively). Hardy-Weinberg equilibrium was observed in all groups.

Results
Considering the ACE polymorphism, it was observed a higher frequency of the DD genotype than expected in the strength experts of the elite group, whereas the strength experts sub-elite athletes had a higher frequency of the ID genotype (χ2 = 8.17; p = 0.01). Subjects with XX genotypes of ACTN3 are more likely to belong to the athlete group when compared to the control group (OR = 1.79, p = 0.04). The DD homozygotes of the ACE are more likely to belong to the elite group with strength phenotypes than the group of sub-elite (OR = 7.96, p = 0.01) and elite strength experts compared to elite endurance (OR = 18.0, p = 0.03). However, no significant differences were observed in the allelic distribution of the polymorphisms evaluated when comparing Elite, sub-elite athletes and controls.

Conclusion
ACE and ACTN3 allele frequencies should be considered with regard to performance influencing factors in Brazilian swimmers.
Streamlining performance prediction: data-driven KPIs in all swimming strokes
Authors: Staunton Craig A. , Romann Michael , Björklund Glenn , Born Dennis-Peter 
Published: February 2024

Abstract
Objective
This study aimed to identify Key Performance Indicators (KPIs) for men’s swimming strokes using Principal Component Analysis (PCA) and Multiple Regression Analysis to enhance training strategies and performance optimization. The analyses included all men’s individual 100 m races of the 2019 European Short-Course Swimming Championships.

Results
Duration from 5 m prior to wall contact (In5) emerged as a consistent KPI for all strokes. Free Swimming Speed (FSS) was identified as a KPI for 'continuous' strokes (Breaststroke and Butterfly), while duration from wall contact to 10 m after (Out10) was a crucial KPI for strokes with touch turns (Breaststroke and Butterfly). The regression model accurately predicted swim times, demonstrating strong agreement with actual performance. Bland and Altman analyses revealed negligible mean biases: Backstroke (0% bias, LOAs − 2.3% to + 2.3%), Breaststroke (0% bias, LOAs − 0.9% to + 0.9%), Butterfly (0% bias, LOAs − 1.2% to + 1.2%), and Freestyle (0% bias, LOAs − 3.1% to + 3.1%). This study emphasizes the importance of swift turning and maintaining consistent speed, offering valuable insights for coaches and athletes to optimize training and set performance goals. The regression model and predictor tool provide a data-driven approach to enhance swim training and competition across different strokes.
Unusual presentation of talonavicular joint pigmented villonodular synovitis: a case report
Authors: Elahifar Omid , Torkaman Ali , Ghaeini Moein , Eslami Arvin 
Published: February 2024

Abstract
Background
Pigmented villonodular synovitis is a rare yet locally invasive disorder impacting synovial tissues. This case report delineates the atypical manifestation of pigmented villonodular synovitis in the talonavicular joint, detailing its diagnostic complexity and successful management.

Case presentation
A 56-year-old Iranian patient with a 4-year history of chronic ankle pain, initially diagnosed with degenerative joint disease post-trauma based on imaging, underwent talonavicular fusion surgery. An unexpected pigmented villonodular synovitis mass was encountered during the procedure. Subsequent interventions encompassed tumor resection, talonavicular joint fusion, and allograft bone grafting. Despite the initial intervention, persistent pain and nonunion necessitated a secondary procedure, involving joint surface curettage and autograft bone grafting. At the 12-month follow-up, the patient remained pain-free without tumor recurrence.

Conclusion
This case report highlights the significance of considering pigmented villonodular synovitis as a crucial differential diagnosis in chronic ankle pain, even when there is evidence of degenerative joint disease and a history of trauma. Magnetic resonance imaging serves a crucial role in accurate diagnosis. Treatment necessitates precise tumor removal, appropriate bone grafting techniques and secure fixation.
Level of evidence: IV.
Variation in the affinity of three representative avian adenoviruses for the cellular coxsackievirus and adenovirus receptor
Authors: Song Yapeng , Tao Mingyue , Liu Lin , Wang Yang , Zhao Zhenchao , Huang Zongmei , Gao Wenming , Wei Qiang , Li Xinsheng 
Published: February 2024

AbstractAccording to previous studies, three representative avian adenoviral strains utilize coxsackievirus–adenovirus receptor (CAR) as a receptor and seem to exhibit diverse binding affinities and modes. Thus, further revealing the exact molecular mechanism underlying the interaction between different FAdVs and the attachment receptor CAR is necessary. In this study, we successfully solved the crystal structure of the FAdV-4 fiber1 knob at 1.6 Å resolution. The interaction between the fibre knob and different domains of CAR was verified by confocal microscopy, coimmunoprecipitation and surface plasmon resonance (SPR) analysis. The fibre knobs of the three representative fowl adenoviruses specifically recognized CAR domain 1 (D1), but the recognition of CAR domain 2 (D2) by chicken embryo lethal orphan (CELO) strains was weak. These results provide insights into the differences in adenovirus‒host cell interactions and have important implications for the exploration of viral invasion mechanisms.
Wood, knots and bark extractives for oak, beech and Douglas fir: a dataset based on a review of the scientific literature
Authors: Richard Béatrice , Bénard Alain , Dumarçay Stéphane , Colin Francis 
Published: February 2024

Key messageWood_db-chemistry gathers information on oak, beech and Douglas fir extractives studied between 1950 and 2020. The data can be used by researchers, stakeholders in the forest-wood sector and the chemical industry to make the most of the extractives and their bioactive properties through knowledge of their diversity in the forest resource. Dataset access is at https://doi.org/10.57745/QZYPUA. Associated metadata are available at https://metadata-afs.nancy.inra.fr/geonetwork/srv/fre/catalog.search#/metadata/4f8c07d2-c0f6-4958-8f74-936054a9870a.
Image fusion research based on the Haar-like multi-scale analysis
Authors: Zhu Xiaoliang , Wen Mengke 
Published: February 2024

AbstractIn view of the serious color and definition distortion in the process of the traditional image fusion, this study proposes a Haar-like multi-scale analysis model, in which Haar wavelet has been modified and used for the medical image fusion to obtain even better results. Firstly, when the improved Haar wavelet basis function is translated, inner product and down-sampled with each band of the original image, the band is decomposed into four sub-images containing one low-frequency subdomain and three high-frequency subdomains. Secondly, the different fusion rules are applied in the low-frequency domain and the high-frequency domains to get the low-frequency sub-image and the high-frequency sub-images in each band. The four new sub-frequency domains are inverse-decomposed to reconstruct each new band. The study configures and synthesizes these new bands to produce a fusion image. Lastly, the two groups of the medical images are used for experimental simulation. The Experimental results are analyzed and compared with those of other fusion methods. It can be found the fusion method proposed in the study obtain the superior effects in the spatial definition and the color depth feature, especially in color criteria such as OP, SpD, CR and SSIM, comparing with the other methods.
Target tracking using video surveillance for enabling machine vision services at the edge of marine transportation systems based on microwave remote sensing
Authors: Li Meiyan , Wang Qinyong , Liao Yuwei 
Published: February 2024

AbstractAutomatic target tracking in emerging remote sensing video-generating tools based on microwave imaging technology and radars has been investigated in this paper. A moving target tracking system is proposed to be low complexity and fast for implementation through edge nodes in a mini-satellite or drone network enabling machine intelligence into large-scale vision systems, in particular, for marine transportation systems. The system uses a group of image processing tools for video pre-processing, and Kalman filtering to do the main task. For testing the system performance, two measures of accuracy and false alarms probability are computed for real vision data. Two types of scenes are analyzed including the scene with single target, and the scene with multiple targets that is more complicated for automatic target detection and tracking systems. The proposed system has achieved a high performance in our tests.