bluuebunny/crossref_metadata_2025_split huggingface dataset:
The TomatoAux/IAATranscription FactorIAA9Is Involved in Fruit Development and Leaf Morphogenesis
Authors: Wang Hua , Jones Brian , Li Zhengguo , Frasse Pierre , Delalande Corinne , Regad Farid , Chaabouni Salma , Latché Alain , Pech Jean-Claude , Bouzayen Mondher 
Published: August 2005

AbstractAuxin/indole-3-acetic acid (Aux/IAA) proteins are transcriptional regulators that mediate many aspects of plant responses to auxin. While functions of most Aux/IAAs have been defined mainly by gain-of-function mutant alleles in Arabidopsis thaliana, phenotypes associated with loss-of-function mutations have been scarce and subtle. We report here that the downregulation of IAA9, a tomato (Solanum lycopersicum) gene from a distinct subfamily of Aux/IAA genes, results in a pleiotropic phenotype, consistent with its ubiquitous expression pattern. IAA9-inhibited lines have simple leaves instead of wild-type compound leaves, and fruit development is triggered before fertilization, giving rise to parthenocarpy. This indicates that IAA9 is a key mediator of leaf morphogenesis and fruit set. In addition, antisense plants displayed auxin-related growth alterations, including enhanced hypocotyl/stem elongation, increased leaf vascularization, and reduced apical dominance. Auxin dose–response assays revealed that IAA9 downregulated lines were hypersensitive to auxin, although the only early auxin-responsive gene that was found to be upregulated in the antisense lines was IAA3. The activity of the IAA3 promoter was stimulated in the IAA9 antisense genetic background, indicating that IAA9 acts in planta as a transcriptional repressor of auxin signaling. While no mutation in any member of subfamily IV has been reported to date, the phenotypes associated with the downregulation of IAA9 reveal distinct and novel roles for members of the Aux/IAA gene family.
Plasmodesmal-Associated Protein Kinase in Tobacco andArabidopsisRecognizes a Subset of Non-Cell-Autonomous Proteins
Authors: Lee Jung-Youn , Taoka Ken-ichiro , Yoo Byung-Chun , Ben-Nissan Gili , Kim Dong-Jin , Lucas William J. 
Published: August 2005

AbstractCell-to-cell communication in plants involves the trafficking of macromolecules through specialized intercellular organelles, termed plasmodesmata. This exchange of proteins and RNA is likely regulated, and a role for protein phosphorylation has been implicated, but specific components remain to be identified. Here, we describe the molecular characterization of a plasmodesmal-associated protein kinase (PAPK). A 34-kD protein, isolated from a plasmodesmal preparation, exhibits calcium-independent kinase activity and displays substrate specificity in that it recognizes a subset of viral and endogenous non-cell-autonomous proteins. This PAPK specifically phosphorylates the C-terminal residues of tobacco mosaic virus movement protein (TMV MP); this posttranslational modification has been shown to affect MP function. Molecular analysis of purified protein established that tobacco (Nicotiana tabacum) PAPK is a member of the casein kinase I family. Subcellular localization studies identified a possible Arabidopsis thaliana PAPK homolog, PAPK1. TMV MP and PAPK1 are colocalized within cross-walls in a pattern consistent with targeting to plasmodesmata. Moreover, Arabidopsis PAPK1 also phosphorylates TMV MP in vitro at its C terminus. These results strongly suggest that Arabidopsis PAPK1 is a close homolog of tobacco PAPK. Thus, PAPK1 represents a novel plant protein kinase that is targeted to plasmodesmata and may play a regulatory role in macromolecular trafficking between plant cells.
Chronic administration of phosphodiesterase 5 inhibitor improves erectile and endothelial function in a rat model of diabetes
Authors: AHN GOOK JUN , YU JAE YOUNG , CHOI SEUL MIN , KANG KYUNG KOO , AHN BYOUNG OK , KWON JONG WON , KANG SUNG KEUN , LEE BYEONG CHUN , HWANG WOO SUK 
Published: August 2005

SummaryThis study was conducted to determine if the long‐term administration of the phosphodiesterase type 5 (PDE 5) inhibitor, DA‐8159, to diabetic rats can ameliorate the development of erectile dysfunction (ED) and endothelial dysfunction. After inducing diabetes with streptozotocin, DA‐8159 was orally administered at a dose of 3 mg/kg or 10 mg/kg for 8 weeks. To examine the effect on erectile response, electrostimulation of the cavernous nerve with the parameters of 3 V, 5 ms, 5 Hz or 10 Hz, was performed to measure the intracavernous pressure (ICP) and mean arterial pressure (MAP). Thoracic aorta relaxation in vitro was evaluated by adding acetycholine (Ach) cumulatively to the bathing medium. In addition, the plasma endothelin‐1 (ET‐1) levels were measured in order to investigate the effect of DA‐8159 on endothelial dysfunction. The area under the curve (AUC) from the ICP/MAP ratio in the 10 Hz stimulation showed a significantly increased AUC after the 10 mg/kg treatment compared with the diabetic group (8891 ± 619 vs. 6316 ± 1016, respectively, p < 0.05). At the 5 Hz frequency, DA‐8159 10 mg/kg also induced a significant increase in the AUC compared with the diabetic control. The maximum ICP/MAP ratio (%) of the 10 mg/kg treatment group was significantly higher in both the 10 Hz and 5 Hz frequency groups (p < 0.05). A treatment of 3 mg/kg tended to increase the AUC and peak ICP/MAP but was not statistically significant. The Ach EC50 value of the diabetic group was significantly higher than in the normal control (120.50 ± 22.90 nm vs. 86.80 ± 9.30 nm, respectively), and 10 mg/kg treatment group showed a significantly lower EC50 value (88.38 ± 19.7 nm). The ET‐1 level was lower in groups treated with DA‐8159, 3 mg/kg and 10 mg/kg treatment induced a statistical difference compared with the diabetic control (1.15 ± 0.34 fmol/mL vs. 2.51 ± 0.55 fmol/mL, respectively, p < 0.05). These results demonstrate that chronic administration of DA‐8159 could attenuate the development of the ED in diabetes and its effect is associated with an improvement in the endothelial function.
From spermatocytes to spermatozoa in an infertile XYY male
Authors: RIVES NATHALIE , MILAZZO JEAN PIERRE , MIRAUX LUDIVINE , NORTH MARIE‐ODILE , SIBERT LOUIS , MACÉ BERTRAND 
Published: August 2005

SummarySex chromosome distribution and aneuploidy as well as germ cell degeneration were evaluated in meiotic and post‐meiotic cells from an infertile XYY male. Sex chromosome distribution was assessed by multicolour fluorescence in situ hybridization on meiotic preparations. Post‐meiotic cell aneuploidy was characterized by a method combining multicolour fluorescence in situ hybridization and immunocytochemistry using the proacrosin‐specific monoclonal antibody (mAb 4D4). TUNEL assay was carried out on seminiferous tubules to evaluate germ cell degeneration. At the prophase stage of the first meiotic division, 63.64% of cells at the pachytene stage carried three sex chromosomes. The ratio of X‐bearing to Y‐bearing spermatids and spermatozoa differed significantly from 1 : 1 with an excess of Y‐bearing spermatids and spermatozoa. The frequency of hyperhaploid XY spermatids was increased in the XYY male, as well as the incidence of YY, XY and disomic 18 ejaculated spermatozoa. A preferential elimination of germ cells by apoptosis occurred in spermatocytes I. The persistence of the extra Y chromosome during meiosis of an XYY male is associated with a high rate of spermatocyte I degeneration and a low rate of aneuploid spermatozoa.
Molecular evaluation of CFTR sequence variants in male infertility of testicular origin
Authors: LARRIBA S. , BONACHE S. , SARQUELLA J. , RAMOS M. D. , GIMÉNEZ J. , BASSAS L. , CASALS T. 
Published: August 2005

SummaryAlthough the involvement of the CFTR gene has been well established in congenital agenesia of vas deferens, its role in non‐obstructive (NOb) infertility is still a matter of debate. In order to definitively define the involvement of the CFTR gene in spermatogenic impairment and a potential synergistic contribution to known genetic and clinical factors, genetic variants in the entire coding sequence and the immediately flanking regions of the CFTR gene, along with a thorough clinical evaluation, were analysed in 83 NOb infertile patients and 87 clinically well‐defined fertile individuals as controls. The results of our study showed no statistical difference between CFTR carrier frequency in the infertile and fertile population. Specifically, the IVS8‐6(5T) allele carrier frequency was similar in NOb infertile patients when compared with fertile men, but it is noteworthy that, when fertile men were classified into having optimal and suboptimal fertility, no 5T allele was found among the 35 men with optimal fertility parameters. In conclusion, extensive CFTR analysis in infertile individuals and fertile population as adequate control definitively excludes the involvement of the CFTR gene variants in sperm production and stresses the importance of carefully identifying those individuals with obstructive defects, in whom CFTR screening will be beneficial.
Feasibility study for the centralized measurement of sperm concentration
Authors: JONCKHEERE JOSEPH , CALLEWAERT NICO , GROOTENHUIS ARIJAN J. , KERSEMAEKERS WENDY M. , COOPER TREVOR G. 
Published: August 2005

SummaryDespite several efforts to standardize methods of semen analysis, sperm count is known to be subject to large interlaboratory differences. This is especially a problem in multicentre clinical trial settings and protocols for the preparation of semen for centralized assessment of sperm concentration are suggested here. The stability of semen has been tested after fixation with formalin at different dilutions and at different temperatures for different sperm concentrations. Sperm concentrations in formalin‐fixed semen (at dilutions 1 + 0.5 to 1 + 19) were stable (<20% difference from original) at all concentrations (0.1 × 106/mL to 100 × 106/mL) for at least 5 days at room temperature and 4 °C. Prolonged stability up to 5 weeks at 4 °C was demonstrated for the lower dilutions (1 + 0.5 and 1 + 1). Freezing of fixed semen samples was unacceptable. These results illustrate that centralized assessment of sperm concentrations is feasible.
Aromatase and oestrogens in human male germ cells
Authors: LAMBARD SOPHIE , CARREAU SERGE 
Published: August 2005

SummaryThe mammalian testis serves two main functions: production of spermatozoa and synthesis of steroids, among them oestrogens are the end products obtained from the irreversible transformation of androgens by aromatase (P450arom). Up today P450arom has been demonstrated in male germ cells of all mammals so far studied (mice, rat, bank vole, bear, monkey). In man Leydig cells and immature germ cells as well as ejaculated spermatozoa express a biologically active aromatase. Moreover germ cells and spermatozoa contain oestrogen receptors (ER‐α and ER‐β) and it is of note that a truncated form of ER‐α is present in spermatozoa. These observations clearly suggest that oestrogens are likely concerned in various stages of male germ cell development.
Zosteriform cutaneous metastases from breast adenocarcinoma
Authors: Bassioukas Konstantinos , Nakuci Mikel , Dimou Sofia , Kanellopoulou Malama , Alexis Ioannis 
Published: August 2005

ABSTRACTCutaneous metastases from breast adenocarcinoma are usually nodular, single or multiple. Their zosteriform distribution is very rare. We present a 54‐year‐old woman with cutaneous zosteriform nodular metastases on the right side of her thorax, and infiltration of the corresponding arm, 3 months after the excision of adenocarcinoma of her right breast.
Sweet's syndrome (acute febrile neutrophilic dermatosis) associated with adenocarcinoma of prostate and transitional cell carcinoma of urinary bladder
Authors: Hussein K , Nanda A , Al‐Sabah H , Alsaleh QA 
Published: August 2005

ABSTRACTAssociation of Sweet's syndrome (SS) with solid tumours is found in about 15% of all malignancy‐associated cases, but an association with two malignancies occurring in the same patient has been rarely reported. In the present report, we describe an 82‐year‐old male with SS in association with adenocarcinoma of the prostate and transitional cell carcinoma of the urinary bladder.
Sildenafil citrate‐aided radiotherapy for the treatment of Kaposi's sarcoma of the penis
Authors: EkmekcI TR , Kendirci M , Kizilkaya O , Koslu A 
Published: August 2005

ABSTRACTWe report a case of classical Kaposi's sarcoma involving multiple sites of the penis in a 62‐year‐old male who was treated with sildenafil citrate‐aided electron beam therapy, resulting in complete resolution of the lesions.
Cutaneous manifestations of familial amyloidotic polyneuropathy
Authors: Rocha N , Velho G , Horta M , Martins A , Massa A 
Published: August 2005

ABSTRACTFamilial amyloidotic polyneuropathy is an autosomal dominant amyloidosis, characterized by the systemic deposition of amyloid with a particular involvement of the peripheral nerves.The disease generally manifests as a severe sensory, motor and autonomic neuropathy. Cardiomyopathy, nephropathy, vitreous opacities and carpal tunnel syndrome may occur in a variable association with the neuropathy. Trophic dermatological lesions are frequent in the more advanced stages of the disease. We examined the skin of 142 patients. The cutaneous manifestations more frequently observed were: xerosis (81.6%), seborrheic dermatitis (21.8%), traumatic and burn lesions (19.7%), acne (18.3%), neurotrophic ulcers (14%) and onychomycosis (10.5%). Among the hepatic transplanted patients (31%), seborrheic dermatitis and acne were the most frequent diagnoses.
Neurofibromatosis of atypical presentation
Authors: Tato BP , Sáez AC , Recuero JLD , Dorado MM , Fernández PR , De Paz FS 
Published: August 2005

ABSTRACTNeurofibromatosis (NF) is considered to be a heterogeneous neuroectodermal disease clinically defined by the presence of neurofibromas, multiple café‐au‐lait spots, intertriginous freckles and Lisch nodules. Mosaicism explains atypical presentations of the disease. Early mutations, before tissue differentiation, give rise to generalized disease. We report an atypical presentation of neurofibromatosis with an unusual distribution of neurofibromas, a peculiar, clinically and pathologically, neurofibroma on the trunk and the association with an ovarian serous cystoadenofibroma.
Painful penile ulceration in a patient with malignant atrophic papulosis
Authors: Aydogan K , Alkan G , Karadogan Koran S , Adim SB , Kiyici M , Tokgoz N 
Published: August 2005

ABSTRACTMalignant atrophic papulosis (MAP) is a rare, obliterating vasculopathy affecting multiple systems, frequently with a poor prognosis. Although cutaneous lesions are often the initial presentation, systemic involvement is also common, usually with a fatal outcome. Involvement of the genitalia is very rare. We describe a 45‐year‐old male patient with multisytemic manifestation of MAP accompanied by painful penile ulceration. The pathogenesis of MAP is not yet fully understood and effective treatment choices are limited. In our case, the combination of pentoxifylline and dipyridamole failed to provide a beneficial effect on the progression of the disease and the patient died due to intestinal and intrathoracic manifestation of MAP. In the present case, attention should be drawn to the following clinical course and therapeutic properties: (i) we describe the second patient in the literature diagnosed with MAP and painful penile ulceration; (ii) to our knowledge, this is the first reported case with oesophageal fistula due to MAP; (iii) we could not confirm the efficacy of pentoxifylline, the recently reported treatment modality, in our patient.
Scleroderma with type III glomerulonephritis and MPO‐ANCA antibodies in the serum
Authors: Herrera‐Esparza R , Aguilar J‐L , Saucedo A , González I , López‐Robles E , Avalos‐Díaz E 
Published: August 2005

ABSTRACTScleroderma is an autoimmune disease characterized by early inflammatory infiltrates followed by fibrosis in the skin and internal organs. CREST is a relatively benign cutaneous variant of scleroderma that features calcinosis, Raynaud's phenomenon, oesophageal dysfunction, sclerodactyly and telangiectases. Glomerulonephritis is a rare association of CREST. We are reporting a patient with CREST who developed glomerulonephritis and had anticentromere and antineutrophil cytoplasmic autoantibodies (ANCA) in her serum.
Lupus erythematosus associated with erythema multiforme: report of two cases and review of the literature
Authors: Aydogan K , Karadogan SK , Balaban Adim S , Tunali S 
Published: August 2005

ABSTRACTRowell's syndrome (RS) is a rare presentation of lupus erythematosus (LE) with erythema multiforme‐like lesions associated with antinuclear, anti‐La (SS‐B)/anti‐Ro (SS‐A) antibodies and rheumatoid factor (RF) positivity. This syndrome is suggested to be a different variant of cutaneous lupus erythematosus by some authors in literature. Here we present a 64‐year‐old woman with LE and a 51‐year‐old woman with LE and Sjögren syndrome (SS) who had erythema multiforme‐like eruptions and discuss the coexistence of lupus erythematosus and erythema multiforme.
Cutaneous larva migrans with folliculitis: a new clinical presentation of this infestation
Authors: Veraldi S , Bottini S , Carrera C , Gianotti R 
Published: August 2005

ABSTRACTTwo patients (a 35‐year‐old woman and a 33‐year‐old man, just returned from trips to Jamaica and Barbados, respectively) presented with cutaneous larva migrans with folliculitis, a rare and atypical clinical presentation of this infestation. Histopathological examination revealed a follicular and perifollicular infiltrate prevalently consisting of lymphocytes and neutrophils, with numerous eosinophils. Both patients were successfully treated with oral albendazole.
Neutrophil dermatosis of the dorsal hands
Authors: Larsen HK , Danielsen AG , Krustrup D , Weismann K 
Published: August 2005

AbstractNeutrophil dermatosis of the dorsal hands (NDDH) is a recently described skin manifestation regarded as a subset of acute febrile neutrophilic dermatotis (Sweet syndrome). We describe 5 cases with pustular and ulcerative plaques and/or bullae and vesicles of the dorsal hands. Three of the patients also had skin changes at sides other than the hands. Associated conditions were found in two patients, one patient treated with hemo‐dialysis for chronic glomerulonephritis, and one patient had suffered from a streptococcal tonsillitis prior to the eruption. Two of the patients had fever, two had neutrophil leucocytosis in peripheral blood and two had elevated sedimentation rates. Histological findings showed signs of vasculitis in biopsies from two of the patients. NDDH is discussed on the basis of prior case reports concerning the subject, and it is concluded that Neutrophil dermatosis of the dorsal hands should be regarded as a localized variety of Sweet syndrome.
An open‐label study of alefacept plus ultraviolet B light as combination therapy for chronic plaque psoriasis
Authors: Ortonne JP , Khemis A , Koo JYM , Choi J 
Published: August 2005

ABSTRACTBackground  Alefacept, a fully human LFA‐3/IgG1 fusion protein, is a selective biological agent approved in the United States for the treatment of chronic plaque psoriasis. In phase 3 trials, clinical improvement and prolonged off‐treatment remission of psoriasis correlated with reductions in circulating memory T cells. Reductions in pathogenic epidermal T cells in psoriatic lesions also have been noted following phototherapy with ultraviolet B (UVB) light. Because alefacept and UVB target T cells in different ways, combination therapy with these two agents may lead to greater efficacy.Objectives  To determine the safety, tolerability, and efficacy trends of combination therapy with alefacept plus UVB light in patients with chronic plaque psoriasis.Methods  In an open‐label, parallel‐group study conducted at two sites, one in France and one in the United States, patients with chronic plaque psoriasis who were candidates for phototherapy received 12‐weekly intramuscular injections of alefacept, 15 mg. In addition, patients were randomized to one of three treatment arms: no UVB treatment, 6‐week UVB treatment, and 12‐week UVB treatment. UVB treatment consisted of narrowband (NB) UVB at the site in France and broadband (BB) UVB at the site in the United States. The 12‐week treatment period was followed by a 12‐week follow‐up period. Clinic visits occurred weekly during treatment and every 2–4 weeks during follow‐up.Results  A total of 60 patients (n = 30/site) were enrolled in the study. Alefacept was well tolerated when administered in combination with UVB treatment and as monotherapy. There was no evidence of increased phototoxicity or photosensitivity with the combination. At each study site, alefacept/UVB provided a higher overall response rate and led to a more rapid onset of response compared with alefacept monotherapy. Of patients who achieved ≥ 50% reduction from baseline Psoriasis Area Severity Index (PASI 50) at 2 weeks after the last dose of alefacept, 75–100% in the combination therapy groups maintained this response throughout follow‐up in the absence of further psoriasis therapy.Conclusions  In patients with chronic plaque psoriasis, combination therapy with alefacept plus short‐term (6–12 weeks) UVB treatment is well tolerated with a trend toward greater and more rapid efficacy than alefacept alone.
Randomized placebo‐controlled trial of a flavonoid‐rich plant extract‐based cream in the treatment of rosacea
Authors: Rigopoulos D , Kalogeromitros D , Gregoriou S , Pacouret JM , Koch C , Fisher N , Bachmann K , Brown M , Schwarz E , Camel E , Katsambas A 
Published: August 2005

ABSTRACTBackground  Biological research suggests that vascular changes may play a major role in rosacea pathogenesis. Chrysanthellum indicum is a plant‐based extract containing a unique combination of phenylpropenoic acids, flavonoids and saponosids, and has a well‐documented effect on vascular wall permeability and increase of the mechanical resistance of capillaries.Objective  To determine the efficacy and safety of a cream containing 1%C. indicum extract with vitamin P properties in the treatment of rosacea.Methods  This study included 246 patients diagnosed clinically as having moderate rosacea. Patients were randomly allocated to C. indicum extract‐based cream (n = 125) and placebo (n = 121) groups. Patients were advised to apply the products on their face twice a day for a 12‐week period. The patients were examined at the end of each 4‐week period. Severity of erythema (graded by reference to six photographs), surface of erythema and rosacea overall severity scores were recorded at each visit on days 0, 28, 56 and 84. Investigators carried out a final efficacy assessment at the end of week 12. Volunteers’ final overall efficacy assessment was recorded in a self‐administered questionnaire. Adverse events were identified through examination, interview and collection of comments in patients’ questionnaires.Results  Treatment with the C. indicum extract‐based cream resulted in significant improvement (P < 0.05) in severity of erythema, overall rosacea severity compared to baseline and placebo, and investigator and patient overall efficacy assessment scores (P = 0.046 and P = 0.001, respectively) compared with placebo scores. Adverse reactions were mild, and did not differ between the C. indicum extract‐based cream and the placebo groups.Conclusion  Chrysanthellum indicum extract‐based cream is an effective and well‐tolerated topical agent for the treatment of moderate rosacea. The mode of action of the active ingredient suggests that additional efficacy might be expected from combination with other topical treatments.