bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ulnar Lengthening for Children with Forearm Deformity from Hereditary Multiple Exostoses: A Retrospective Study from A Tertiary Medical Center
Authors: Wang Shuo , Herman Bomansaan , Wu Yiheng , Lei Ming , Mutasa Blessing , Hong Pan , Li Jin 
Published: November 2023

Abstract

Background Patients with hereditary multiple exostosis (HME) usually present with forearm deformity with or without radial head dislocation. Ulna lengthening has been proposed to address this condition. Exostosis resection plus ulna lengthening has been adopted in our hospital since 2008, and patients with this condition were retrospectively reviewed. Herein, we aimed to investigate the optimal timing and clinical outcomes of this surgical approach.Methods In all, twenty-seven patients (32 forearms), including 18 boys and 9 girls, were enrolled in our study from July 2014 to September 2020. We divided the patients into 4 groups based on the age when they received surgery and the status of the radial head. Pronation and supination of the forearm, flexion and extension of the elbow, wrist ulnar deviation and wrist radial deviation, and radiological parameters including ulnar length (UL), ulnar variance (UV), the percentage of radial bowing (RB/RL), radio articular angle (RAA) and carpal slip (CS), were assessed and recorded.Results The mean UL was significantly improved after surgery in four Groups (P༜0.05). For the patients with radial head dislocation, the juniors demonstrated better improvement in % RB and RAA (p༜0.05, p = 0.003 and 0.031).Conclusion Exostosis resection and ulna lengthening with unilateral external fixation can effectively improve the function and radiological parameters of forearm deformity in HME children. For patients with radial head dislocation, early surgery can achieve better results. For patients not associated with radial head dislocation, we recommend regular follow-up and surgical treatment after 10 years of age.
Method of Home Tube Feeding and 2–3-year Neurodevelopmental Outcome
Authors: Dupont Tara , Fisher Allison , Ling Con Yee , Ermath Anna , Brinker Kristin 
Published: November 2023

Abstract

Objective: To describe the Bayley Scales of Infant Development 3rd Edition (Bayley-III) of infants discharged home receiving tube feeds
Study Design: Retrospective review of infants discharged with nasogastric or gastrostomy tube feeds and completed a Bayley-III assessment at 2-3-years of age through a neonatal follow-up program. Results were reported using descriptive statistics.
Results: Of infants discharged with nasogastric feeds, median Bayley-III scores were in the low-average to average range and full oral feeds were achieved in 75%. Of infants discharged with gastrostomy tube feeds, median Bayley-III scores were in the extremely low range and full oral feeds were achieved in 36%. Our data set did not demonstrate a distinct patient demographic that correlated to the type of feeding tube at discharge.
Conclusion: Neurodevelopmental outcome at 2–3 years does not appear to be negatively impacted by the decision to discharge an infant from the NICU with home NG feedings.
Clostridium butyricum Inhibits the Inflammation in Children with Primary Nephrotic Syndrome by Regulating Th17 / Tregs Balance via Gut-Kidney Axis
Authors: Li Ting , Ma Xiaolong , Wang Ting , Tian Wenyan , Liu Jian , Shen Wenke , Liu Yuanyuan , Li Yiwei , Zhang Xiaoxu , Ma Junbai , Zhang Xiaoxia , Ma Jinhai , Wang Hao 
Published: November 2023

Abstract
Background
Primary nephrotic syndrome (PNS) is a common glomerular disease in children. Clostridium butyricum (C.butyricum), a probiotic producing butyric acid, exerts effective in regulating inflammation. This study was designed to elucidate the effect of C.butyricum on PNS inflammation through the gut-kidney axis.
Methods
BALB/c mice were randomly divided into 4 groups: normal control group (CON), C.butyricum control group (CON + C.butyricum), PNS model group (PNS) and PNS with C.butyricum group (PNS + C.butyricum). The PNS model was established by a single injection of doxorubicin hydrochloride (DOX) through the tail vein. After 1 week of modeling, the mice were treated with C.butyricum or normal saline for 6 weeks. At the end of the experiment, the mice were euthanized and associated indications were investigated.
Results
Since the successfully modelling of the PNS, the 24h urine protein, blood urea nitrogen (BUN), serum creatinine (SCr), urine urea nitrogen (UUN),urine creatinine (UCr), lipopolysaccharides (LPS), pro-inflammatory interleukin (IL)-6, IL-17A were increased, the kidney pathological damage was aggravated, while a reduction of body weights of the mice and the anti-inflammatory IL-10 significantly reduced. However, these abnormalities could be dramatically reversed by C.butyricum treatment. The crucial Th17-Tregs axis in PNS inflammation also was proved to be effectively regulated by C.butyricum treatment. This probiotic intervention notably affected the expression levels of signal transducer and activator of transcription 3 (STAT3), Heme oxygenase-1 (HO-1) protein, and retinoic acid-related orphan receptor gamma t (RORγt). 16S rRNA sequencing showed that C.butyricum could regulate the composition of intestinal microbial community and found Proteobacteria was more abundant in urine microorganisms in mice with PNS. Short-chain fatty acids (SCFAs) were measured and showed that C.butyricum treatment increased contents of acetic acid, propionic acid, butyric acid in feces and acetic acid, valeric acid in urine. Correlation analysis showed that there was a closely complicated correlation among inflammatory indicators, metabolic indicators, microbiota and associated metabolic SCFAs in gut-kidney axis.
Conclusion
C.butyricum regulates Th17 / Tregs balance via the gut-kidney axis to suppress the immune inflammatory response in mice with PNS, which may potentially contribute to a novel, inexpensive, and safe therapeutic reagent for the control of the disease.
Comparison of the formula accuracy for calculating multifocal intraocular lens power in Asian patients
Authors: Kim Jinchul , JOONSUNG PARK , JO YOONJUNG 
Published: November 2023

Abstract
This study evaluated the accuracy of newer formulas (Barrett Universal II, EVO 2.0, Kane, Hoffer QST, and PEARL-DGS) and the Haigis formula in Asian patients with the Alcon TFNT multifocal intraocular lens. In total, 3,100 randomly selected eyes of 3,100 patients were retrospectively reviewed. After constant optimization, the standard deviation (SD) of the prediction error was assessed for the entire group, and the root mean square error (RMSE) was compared for short and long axial length (AL) subgroup analysis. The Cooke-modified AL (CMAL) was experimentally applied to the Haigis formula. All the newer formulas performed well, but they did not significantly outperform the Haigis formula. In addition, all the newer formulas exhibited clinically significant myopic outcomes in long eyes. Application of the CMAL to the Haigis formula with single constant optimization produced similar behavior and higher correlation with the newer formulas. The CMAL-applied triple-optimized Haigis formula yielded a smaller SD than the newer formulas. The AL modification algorithms such as the CMAL used in newer formulas to cope with optical biometry's overestimation of the AL in long eyes seemed to overcompensate, particularly in the long eyes of the Asian population.
Establishment and application of blocking ELISA method for detection of against Duck reovirus σC antibody
Authors: Han Jingyi , Huang Cong , Li Hongchang , Zhao Lu , Li Jia , Huang Yuanling , Tang Qijiang , Liu Yingnan , Liu Jingyi , Chen Hongjun , Shu Gang , Chen Zongyan 
Published: November 2023

Abstract
Duck reovirus disease is a duck-transmitted disease characterized by homorrhage and necrosis of the livers and spleens. Blocking ELISA has high sensitivity and simple operation, which can be used as a serological test for duck infection, to improve the detection efficiency of DRV infection. Duck reovirus σC protein is the main protein involved in the body's immune system, which can induce the production of neutralizing antibodies. Currently, there is no established blocking ELISA detection method based on this protein. This study aimed to produce and characterize σC monoclonal antibodies with the ultimate goal of developing a monoclonal antibody-based blocking ELISA for DRV antibody detection. This established detection method had no response to the serum after infection with ARV and MDRV, so it can also be used to distinguish DRV from ARV and MDRV infections. To evaluate the performance of the detection method, 87 serum samples (53 negative and 34 positive samples) were analyzed and receiver-operating characteristic (ROC) analysis was applied to determine the Cut-Off value. According to ROC analysis, the area under the curve (AUC) was 0.9789 (95% confidence interval: 95.13 to 100%), the Cut-Off value was set to 15.49%. The coefficients of inter- and intra-batches were < 10%, indicating the good repeatability of the method. The maximum dilution of positive standard serum detected by this blocking ELISA method was 1:512 and did not cross-react with other duck-derived viruses, illustrating strong specificity. In conclusion, the σC mAb-based blocking ELISA detection method established in this study has high sensitivity and specificity, which provides a reliable and useful tool for on-site surveillance and epidemiological studies in duck flocks.
Potential Involvement of beta-lactamase homologous proteins in resistance to beta-lactam antibiotics in gram-negative bacteria of the ESKAPEE group
Authors: Souza Joyce , Vieira Alexandre Zanatta , Santos Hellen Geremias dos , Faoro Helisson 
Published: November 2023

Abstract
Background
Enzymatic degradation mediated by beta-lactamases constitutes one of the primary mechanisms of resistance to beta-lactam antibiotics in gram-negative bacteria. This enzyme family comprises four molecular classes, categorized into serine beta-lactamases (Classes A, C, and D) and zinc-dependent metallo-beta-lactamases (Class B). Gram-negative bacteria producing beta-lactamase are of significant concern, particularly due to their prevalence in nosocomial infections. A comprehensive understanding of the evolution and dissemination of this enzyme family is essential for effective control of these pathogens.
Results
In this study, we conducted the prospecting, phylogenetic analysis, and in silico analysis of beta-lactamases and homologous proteins identified in 1827 bacterial genomes with phenotypic data on beta-lactam resistance. These genomes were distributed among Klebsiella pneumoniae (45%), Acinetobacter baumannii (31%), Pseudomonas aeruginosa (14%), Escherichia coli (6%), and Enterobacter sp. (4%). Using an HMM profile and searching for conserved domains, we mined 2514, 8733, 5424, and 2957 proteins for molecular classes A, B, C, and D, respectively. This set of proteins encompasses canonical subfamilies of beta-lactamases as well as hypothetical proteins and other functional groups. Canonical beta-lactamases were found to be phylogenetically distant from hypothetical proteins, which, in turn, are closer to other representatives of the penicillin-binding-protein (PBP-like) and metallo-beta-lactamase (MBL) families. Motif analysis revealed that, in general, catalytic residues are less conserved in homologous groups than in beta-lactamases. Comparison of the frequency of presence of protein groups in genomes of resistant strains in relation to sensitive ones, indicated that some groups of homologous proteins of classes B and C are more frequent in genomes of resistant than sensitive strains, particularly in those resistant to carbapenems.
Conclusions
The beta-lactamase-like domain is present in various proteins from the PBP-like and MBL superfamilies, widely distributed in gram-negative bacteria of the ESKAPEE group. Despite hypothetical proteins with this domain not demonstrating beta-lactamase characteristics, they are closely related to homologous families that exhibit enzymatic promiscuity against beta-lactam antibiotics beyond their traditional functions. The association of these homologous enzymes with canonical beta-lactamases and the selective pressure from the extensive use of beta-lactam antibiotics may favor the eventual optimization of these functions.
“They seemed to be like cogs working in different directions”: a longitudinal qualitative study on Long COVID healthcare services in the United Kingdom from a person-centred lens
Authors: Fang Chao , Baz Sarah Akhtar , Sheard Laura , Carpentieri JD 
Published: November 2023

Abstract
Background
Long COVID (LC) has presented significant challenges to healthcare, as people with persistent and fluctuating symptoms often face prolonged uncertainty when seeking medical support. Negative experiences with healthcare systems and trauma from previous health conditions may further compound this uncertainty. Despite an increasingly rich picture of LC healthcare challenges being captured, qualitative studies on LC so far have primarily focused on individual experiences rather than examining the structural aspects of healthcare
Methods
To better understand the healthcare experiences of those with LC, we conducted a longitudinal qualitative study with 80 participants and over 10 healthcare practitioners in the UK. We collected a total of 178 interviews (with some attrition) across two rounds, from November 2021 to March 2022, and from June to October 2022.
Results
Adopting a framework of person-centredness that acknowledges and supports interconnected individual, relational, and existential needs in healthcare, we explored LC healthcare experiences across primary, secondary, and specialist integrated care. Through this approach, we identified three overarching themes. Theme 1 focuses on the continued hurdle of accessing primary care as the first point of contact for LC healthcare; theme 2 highlights the complexity of navigating secondary care; and theme 3 captures the unique challenges of propagating LC integrated care. These themes were interconnected, with individuals often bouncing between systems and practitioners cooperating across their remits.
Conclusion
Using a person-centred lens allowed us to identify the multifaceted needs of LC sufferers and understand how these needs shape individual health and healthcare experiences in a nuanced way, considering their identity as a key source shaping their healthcare needs. The study aimed to explore how person-centred care can be delivered more effectively and sustainably across different healthcare settings and over time to better meet the needs of LC sufferers.
Medical History and Preoperative Coagulation Profile as Predictors of Outcomes in Elective Neurosurgery: A Meta-Analysis of 78,909 patients
Authors: Pichardo-Rojas Pavel S. , Calvillo-Ramirez Alejandro , Rio-Martinez Christopher J. , Fukumoto-Inukai Kenzo A. , Gonzalez-Hernandez Diana , Casas-Huesca Ana Paulina , Villarreal-Guerrero Cristina , Shah Siddharth 
Published: November 2023

Abstract
Introduction: In patients with unremarkable medical history, the role of comprehensive preoperative hemostasis screening in elective neurosurgical procedures remains debated. Comprehensive medical history has shown to be non-inferior to coagulation profile to evaluate surgical outcomes. This study aims to evaluate the predictiveness of preoperative coagulation screening and medical history for surgical outcomes.
Objective: We conducted a meta-analysis to analyze the predictiveness of preoperative hemostasis screening and clinical history for patient outcomes in elective neurosurgical procedures.
Methods: Databases were searched until April 22nd 2023 for observational cohort studies that reported preoperative hemostasis screening and clinical history prior to elective neurosurgical procedures. Outcomes of interest included postoperative transfusion, mortality, and complications. Pooled relative risk ratios (RR) were analyzed using random-effects models.
Results: Out of 604 studies, 3 cohort studies with a patient population of 78,909 met our inclusion criteria. Prolonged PTT (RR=1.42, 95%CI=1.14-1.77, p=0.002), elevated INR (RR=2.01, 95%CI=1.14-3.55, p=0.02), low platelet count (RR=1.58, 95%CI=1.34-1.86, p<0.00001), and positive bleeding history (RR=2.14, 95%CI=1.16-3.93, p=0.01) were associated with postoperative transfusion risk. High PTT (RR=2.42, 95%CI=1.24-4.73, p=0.010), High INR (RR=8.15, 95%CI=5.97-11.13, p<0.00001), low platelet count (RR=4.89, 95%CI=3.73-6.41, p<0.00001), and bleeding history (RR=7.59, 95%CI=5.84-9.86, p<0.00001) were predictive of mortality. Prolonged PTT (RR=1.53, 95%CI=1.25,1.86; p=<0.0001), a high INR (RR=3.41, 95%CI=2.63,4.42; p=< 0.00001), low platelets (RR=1.63, 95%CI=1.40,1.90; p=<0.00001), and medical history (RR=2.15, 95%CI=1.71,2.71; p=<0.00001) were predictive of complications.
Conclusion: Medical history was a non-inferior predictor to coagulation profile for postoperative transfusion, mortality, and complications in elective neurosurgery. Standardized clinical risk stratification tools, and cost-effective alternatives should be explored to promote affordable-patient care.
Assessment and Characterisation of Coronary Disorder in a Rheumatoid Arthritis Cohort: A Cross-Sectional Study
Authors: Guillaud-Rollin S. , Djaileb L. , De Leris N. , Canu M. , Barone-Rochette G. , Gaudin P. , Romand X. , Gastaldi R , Baillet A 
Published: November 2023

Abstract
Purpose
To assess the prevalence of macrovascular or microvascular coronary dysfunction in a cohort of rheumatoid arthritis patients with a heart score > 1%.
Materials and methods
We included, in a cross-sectional study, RA patients treated with biologic or targeted synthetic Disease Modifying Anti Rheumatic Drug, without history of cardiovascular disease, ≥ 50 years old and a pathological Heart Score > 1%. First, myocardial perfusion reserve (MPR), the ratio of myocardial blood flow in stress to myocardial blood flow at rest, was assessed with single photon emission computed tomography (SPECT). RA patients with impaired MPR < 2 were referred for invasive coronary angiography to assess macrovascular or microvascular coronary dysfunction. Demographics, RA outcomes and cardiovascular risk factors were recorded. A multivariate analysis was performed with variables significantly associated with coronary dysfunction.
Results
A total of 108 RA patients were screened for eligibility. Sixty patients were included. A total of 37/60 (61%) patients had impaired MPR on SPECT, of whom one patient presented significant ischemia on myocardial perfusion imaging. According to invasive coronary angiography, 17/31 (54%) had coronary microvascular dysfunction, 7/31 (22%) had macrovascular dysfunction and 7/31 (22%) had no coronary dysfunction. A total of 23/31 patients (74%) had cardiovascular medical treatment optimization. RA characteristics significantly associated with coronary dysfunction were higher disease activity and shorter disease duration.
Conclusion
This is the first study to reveal, on invasive coronary angiography, a high proportion of microvascular dysfunction in RA patients with impaired MPR. Coronary dysfunction screening could be considered in RA patients with a heart score > 1% (ESC guidelines), as SPECT and invasive coronary angiography showed coronary dysfunction in 44% of RA patients, which led to substantial cardiovascular treatments.
Trial registration
: Ethics approval was obtained from Comité de protection des personnes Sud-Est (22.04002.000195). Participants gave informed consent.
Abies pinsapo and forest fires: a long history of surviving extreme events in Sierra Bermeja (SW Baetic System)
Authors: Gómez-Zotano José , Pardo-Martínez Rubén , Olmedo-Cobo José Antonio , Martos-Martín Javier 
Published: November 2023

Abstract
Background Abies pinsapo is a species classified as endangered and currently occupies a very small and fragmented area of distribution in the southwest of the Baetic System. The uniqueness of these relict forests has motivated their study from different perspectives; however, the paleoecological characterisation of this fir is still clearly deficient. Consequently, there is hardly any information on the impact that fire has had on this conifer during the Holocene, nor the real effect that this disturbance has had on the Spanish fir in recent decades. To fill this knowledge gap, the present research uses the pedoanthracological network developed over the last few years in one of the natural habitats of the Spanish fir forest most affected by fire: Sierra Bermeja. This discipline is based on the analysis of the charcoals present in the natural soils, which are an unexplored resource and particularly useful for the reconstruction of paleo-fires and the knowledge of the dynamics of the vegetation. This paleoecological approach has also been complemented by the mapping of recent forest fires. Results The identification of Abies charcoal in places where this taxon is not present today supports the idea that the Spanish fir was more widely distributed in the past. Likewise, the available dates have revealed the existence of up to 28 events of forest fires that have affected the Spanish fir during the Holocene. The recurrence of fire could have caused its local extinction in the Sierra Palmitera. This regressive dynamic of this fir as a consequence of fire, as can be inferred from the cartography carried out, has been particularly intense during the last few decades. Conclusions Results report on the important role played by fire in the dynamics of the serpentine-soil Spanish firs of Sierra Bermeja from the Last Glacial Maximum to the present day. This is a phenomenon that has intensified in recent decades, affecting the Spanish fir in a very worrying way. To help ensure effective protection for the most important serpentine-tolerant ecosystem in Spain it is essential to include the whole of Sierra Bermeja in the Spanish National Park Network.
DHODH inhibition represents a therapeutic strategy and improves abiraterone treatment in castration-resistant prostate cancer
Authors: Wang Junjian , shaoqiang guo , miaomiao Miao , yufeng Wu , Qinyan Wu , dongyue Pan , Zhanfang Kang , Jianwen Zeng , Liu Chengfei , Guoping Zhong 
Published: November 2023

Abstract
Castration-resistant prostate cancer (CRPC) is an aggressive disease with poor prognosis, and there is an urgent need for more effective therapeutic targets to address this challenge. Here, we showed that Dihydroorotate dehydrogenase (DHODH), an enzyme crucial in the pyrimidine biosynthesis pathway, is a promising therapeutic target for CRPC. The transcript levels of DHODH were significantly elevated in prostate tumors and were negatively correlated with the prognosis of patients with prostate cancer. DHODH inhibition effectively suppressed CRPC progression by blocking cell cycle progression and inducing apoptosis. Notably, treatment with BAY2402234 activated androgen biosynthesis signaling in CRPC cells. However, the combination treatment with BAY2402234 and abiraterone decreased intratumoral testosterone levels and induced apoptosis, which inhibited the growth of CWR22Rv1 xenograft tumors and patient-derived xenograft organoids. Taken together, these results establish DHODH as a key player in CRPC and as a potential therapeutic target for advanced prostate cancer.