bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection and Quantification of Polymorphic MICA and MICB Molecules in Immunoassays: Initial Insights
Authors: Stolbovaya A. Yu. , Pinevich A. A. , Gryazeva I. V. , Krutetskaya I. Yu. , Zharinov G. M. , Morozova M. A. , Kneev A. Yu. , Terekhina L. A. , Ishchuk S. A. , Shashkova O. A. , Vartanian N. L. , Samoylovich M. P. , Bezhenar V. F. , Sokolov D. I. , Selkov S. A. , Smirnov I. V. 
Published: February 2025

ABSTRACTThe MICA and MICB molecules, expressed on the cell membrane in response to cellular stress or cancer transformation, pose significant challenges for immunoassays. They exhibit high sequence and structural similarity, alongside considerable allelic polymorphism, with 291 and 53 known protein sequences, respectively. Some researchers treat MICA and MICB as a unified target because of their structural and functional similarities, while others distinguish between them. However, which approach is superior and under what circumstances remains unknown. Moreover, information about assays' reactivity with MICA and MICB allelic variants is often missing. In this study, we developed 10 monoclonal antibodies (mAbs) and two sandwich ELISAs for the detection and quantification of these molecules. We assembled a panel of recombinant proteins representing the diversity of MICA and MICB in the European population and profiled the reactivities of the mAbs and ELISAs. The performance of these sandwich ELISAs was evaluated using samples from prostate cancer patients and pregnant women experiencing premature rupture of membranes. Our study assessed the impact of MICA and MICB polymorphism on their detection and quantification by immunological methods, providing evidence to support differential or non‐differential approaches for their detection.
Identification of Two Novel Alleles HLA‐B*14:136 and HLA‐B*58:159 by Next‐Generation Sequencing
Authors: Guan Qingdong , Jacobson Kristin , Gentile Aggie , Suniga Hernan 
Published: February 2025

ABSTRACTTwo novel HLA‐B alleles, HLA‐B*14:136 and ‐B*58:159, identified by NGS during the routine HLA typing.
The Novel HLA‐DQB1*02:01:01:27 Allele Identified by HLA‐Targeted HiFi Sequencing in a Chinese Individual
Authors: Li Yankun , Zhao Yuanli , Han Yue , Chen Yingjie , Gao Peng 
Published: February 2025

ABSTRACTHLA‐DQB1*02:01:01:27 differs from HLA‐DQB1*02:01:01:01 by the deletion of a single Thymine nucleotide in intron 2.
PlantGENE: Advancing plant transformation through community engagement
Authors: Malzahn Aimee A. , Kaeppler Heidi , Gordon‐Kamm William , Lee Keunsub , Taylor Nigel , Veena Veena , Parrott Wayne , Van Eck Joyce 
Published: February 2025

SUMMARYPlant transformation is an important part of plant research and crop improvement. Transformation methods remain complex, labor intensive, and inefficient. PlantGENE is a community of scientists from academia, industry, non‐profit research institutes, and government organizations working to improve plant transformation. PlantGENE hosts virtual training, interactive webinars, and a website with career opportunities, directories, and more. The plant science community has shown great interest and support for PlantGENE.
The DNA methylomes of Echinochloa species provide insights into polyploidization‐driven adaptation and orphan crop domestication
Authors: Chu Qinjie , Gong Xiaojiao , Hu Yiyu , Zhu Qian‐Hao , Fan Longjiang , Ye Chu‐Yu 
Published: February 2025

SUMMARYThe genus Echinochloa (Poaceae) contains problematic weeds worldwide and two domesticated orphan crops. In this study, through sequencing DNA methylomes and transcriptomes, we aimed to reveal the epigenome changes and their relationship with gene expression in Echinochloa species during their polyploidization and domestication. Compared with hexaploid crop bread wheat, we found common and distinctive methylation patterns in hexaploid Echinochloa crus‐galli. More diverse methylation patterns were uncovered during hexaploidization of E. crus‐galli, suggesting more plasticity of the weed genome, which might contribute to its environmental adaptation. In addition, less changes in DNA methylation were observed in the two cultivated Echinochloa species, as compared with rice, indicating incomplete domestication of the Echinochloa orphan crops. Our results provide new insights into plant polyploidization and orphan crop domestication from an epigenomic perspective.
Full Galois groups of polynomials with slowly growing coefficients
Authors: Bary‐Soroker Lior , Goldgraber Noam 
Published: February 2025

AbstractChoose a polynomial uniformly at random from the set of all monic polynomials of degree with integer coefficients in the box . The main result of the paper asserts that if grows to infinity, then the Galois group of is the full symmetric group, asymptotically almost surely, as . When grows rapidly to infinity, say , this theorem follows from a result of Gallagher. When is bounded, the analog of the theorem is open, while the state‐of‐the‐art is that the Galois group is large in the sense that it contains the alternating group (if , it is conditional on the Extended Riemann Hypothesis). Hence the most interesting case of the theorem is when grows slowly to infinity. Our method works for more general independent coefficients.
Nonlinear Damping Amplifier Friction Bearings
Authors: Chowdhury Sudip , Adhikari Sondipon 
Published: February 2025

Abstract
This article introduces damping amplifiers integrated into the core material of conventional nonlinear friction base isolators to improve their vibration attenuation capabilities and mitigate their shortcomings. Four new types of damping amplifier friction bearings are introduced: levered, nested, compound, and damping. The H2 optimization strategy is utilized to obtain the closed-form analytical solution for the optimized designed parameters. The analytical investigation confirms the efficiency of the recently obtained closed-form equations for the optimal design parameters. The frequency response function was used to develop closed-form formulas for the isolator and main structure’s dynamic responses. The harmonic and random white noise excitation served as the basis. Furthermore, numerical analysis by considering real earthquake load has confirmed the novel isolators’ efficiency. The vibration reduction capacities of the H2 optimized damping amplifier friction bearings, compound damping amplifier friction bearings, nested damping amplifier friction bearings, and levered damping amplifier friction bearings are 76.1% superior to the optimum conventional base isolator. These findings demonstrate how the proposed designs may improve structural resistance to dynamic loads.
Aortic Dissection During and After Investigation Using CFD Modelling
Authors: Al-Rawi Mohammad , Belkacemi Djelloul , Khashram Manar 
Published: February 2025

Abstract
Cardiovascular disease is a leading cause of death in economically developed countries, despite significant improvements to monitoring and treatment technologies. Early diagnosis for the purposes of targeting interventions is critical, as advanced stages of the disease are frequently fatal. One complication of advanced cardiovascular disease is aortic dissection, which is a catastrophic failure of the endothelial wall. Computational modelling can be used to create 3D models with clinical data, and the clinical blood and pressure waveforms’ model can be used to form boundary conditions for assessing wall shear stress (WSS) along the aorta to predict the development of cardiovascular disease. In this study, we present patient-specific data for a rare case of severe Type A aortic dissection, which was fatally simulated using the k-omega with shear stress transport (SST), turbulent and non-Newtonian assumption. The investigated data for the patient was based on CT scan images taken nine months apart. Both cases were compared regarding pressure waveforms at the ascending and descending aorta and the aortic arch. The results for the before-dissection situation show that the pressure waveform at the ascending aorta is higher, and the systolic pressure is lagging at the descending aorta. For the after-dissection situation, we observe the same outcome; however, the amplitude for the waveform (systolic pressure) at the ascending aorta increased for the false lumen by 25% compared to the true lumen by 3%; also, the waveform peak (systolic) is leading by 0.01s.
High-sensitivity C-reactive protein and risk of retinal artery occlusion and ischaemic stroke: a cross-cohort study
Authors: Wang Yaxin , Zhang Xiayin , Liang Anyi , Niu Yongyi , Chen Daiyu , Du Zijing , Wu Wei , Zhang Feng , Wu Guanrong , Fang Ying , Shang Xianwen , Zhu Zhuoting , Hu Yijun , Chen Xiangjun , Yu Honghua , Hu Lidan 
Published: February 2025

AimsPrevious studies have suggested an association between retinal artery occlusion (RAO) and ischaemic stroke (IS), both associated with elevated inflammatory factors. However, the role of high-sensitivity C-reactive protein (hs-CRP) in the sequential onset of these two diseases is still unclear. Based on this evidence, we evaluated the association of hs-CRP with RAO and IS.MethodsWe examined hs-CRP from both the large multicentre cohort study UK Biobank and Chinese Retinal Artery Occlusion study. Cox proportional hazard models were used to study the association of hs-CRP with incident RAO and IS during the long-term follow-up in the UK Biobank. Logistic regression analysis was employed to assess the cross-sectional relationship between hs-CRP with RAO and IS in the Chinese cohort. A restricted cubic spline (RCS) approach was employed to evaluate potential non-linear associations of hs-CRP with IS.ResultsAfter exclusions, the analysis included 459 188 participants from the UK and 338 participants from China. Over a median follow-up of 12.2 years, 136 cases of incident RAO and 3206 cases of incident IS events were recorded in the UK Biobank. After multivariable adjustment, higher hs-CRP (per 10 mg/L) level was associated with increased risks of RAO (HR: 1.34, 95% CI: 1.01 to 1.76) and IS (HR: 1.24, 95% CI: 1.17 to 1.33). RCS analysis revealed a significant non-linear relationship between hs-CRP levels and incident IS (Pnon-linear<0.001). Furthermore, RAO patients with higher hs-CRP levels were more likely to be combined with IS (2.81 mg/L vs 10.14 mg/L, p<0.001). In the Chinese cohort, the association between hs-CRP with RAO and IS was further confirmed. Higher hs-CRP (per 1 mg/L) level was associated with increased risks of RAO (OR: 1.43, 95% CI: 1.15 to 1.78) and IS (OR: 1.13, 95% CI: 1.03 to 1.24).ConclusionsOur findings underscore hs-CRP as a robust risk factor for both RAO and IS. Controlling hs-CRP levels might reduce the incidence of RAO and secondary stroke.
Association of glycemic control with Long COVID in patients with type 2 diabetes: findings from the National COVID Cohort Collaborative (N3C)
Authors: Soff Samuel , Yoo Yun Jae , Bramante Carolyn , Reusch Jane E B , Huling Jared Davis , Hall Margaret A , Brannock Daniel , Sturmer Til , Butzin-Dozier Zachary , Wong Rachel , Moffitt Richard 
Published: February 2025

IntroductionElevated glycosylated hemoglobin (HbA1c) in individuals with type 2 diabetes is associated with increased risk of hospitalization and death after acute COVID-19, however the effect of HbA1c on Long COVID is unclear.ObjectiveEvaluate the association of glycemic control with the development of Long COVID in patients with type 2 diabetes (T2D).Research design and methodsWe conducted a retrospective cohort study using electronic health record data from the National COVID Cohort Collaborative. Our cohort included individuals with T2D from eight sites with longitudinal natural language processing (NLP) data. The primary outcome was death or new-onset recurrent Long COVID symptoms within 30–180 days after COVID-19. Symptoms were identified as keywords from clinical notes using NLP in respiratory, brain fog, fatigue, loss of smell/taste, cough, cardiovascular and musculoskeletal symptom categories. Logistic regression was used to evaluate the risk of Long COVID by HbA1c range, adjusting for demographics, body mass index, comorbidities, and diabetes medication. A COVID-negative group was used as a control.ResultsAmong 7430 COVID-positive patients, 1491 (20.1%) developed symptomatic Long COVID, and 380 (5.1%) died. The primary outcome of death or Long COVID was increased in patients with HbA1c 8% to <10% (OR 1.20, 95% CI 1.02 to 1.41) and ≥10% (OR 1.40, 95% CI 1.14 to 1.72) compared with those with HbA1c 6.5% to <8%. This association was not seen in the COVID-negative group. Higher HbA1c levels were associated with increased risk of Long COVID symptoms, especially respiratory and brain fog. There was no association between HbA1c levels and risk of death within 30–180 days following COVID-19. NLP identified more patients with Long COVID symptoms compared with diagnosis codes.ConclusionPoor glycemic control (HbA1c≥8%) in people with T2D was associated with higher risk of Long COVID symptoms 30–180 days following COVID-19. Notably, this risk increased as HbA1c levels rose. However, this association was not observed in patients with T2D without a history of COVID-19. An NLP-based definition of Long COVID identified more patients than diagnosis codes and should be considered in future studies.
Diabetes beliefs, perceived risk and health behaviours: an embedded mixed-methods analysis from the Richmond Stress and Sugar Study
Authors: Khosrovaneh Katherine , Kalesnikava Viktoryia A , Mezuk Briana 
Published: February 2025

IntroductionEfforts to increase engagement with diabetes prevention programmes largely focus on increasing diabetes awareness, with the logic that risk knowledge will motivate behaviour change. However, the salience of perceived risk as it relates to diabetes prevention is contested. The goal of this cross-sectional, embedded mixed-methods study was to examine the relationships between perceived risk, diabetes beliefs and prevention behaviours among adults at elevated risk of type 2 diabetes.MethodsData come from the Richmond Stress and Sugar Study (n=125). Diabetes beliefs (ie, internal, chance, powerful others) were assessed using the Multidimensional Health Locus of Control. Preventive behaviours (eg, changing diet, exercise, tobacco, alcohol) were measured by self-report. Perceived risk of developing diabetes was measured using a probability scale (0%–100%). Logistic and Poisson regression models quantified the relationships between beliefs, behaviours and perceived risk. Qualitative themes regarding challenges and facilitators to preventive behaviours were abstracted from open-ended questions and summarised using content analysis.ResultsPerceived risk of developing diabetes was low (median: 35% likelihood) and only 10% of participants had ever attended a prevention class. None of the diabetes belief domains were significantly associated with either engagement in preventive behaviours or perceived diabetes risk. Perceived diabetes risk was not associated with engagement in preventive behaviours; however, having a family history of diabetes was strongly related to perceived risk (OR: 3.35, 95% CI: 1.42 to 7.86). Qualitative facilitators and barriers of preventive behaviours reflected psychosocial factors (eg, determination, stress, preferences) and resources (eg, social support, time, overall health).ConclusionsPerceptions of risk and health beliefs are not correlated with engagement in preventive behaviours among adults at clinically elevated risk of diabetes. Awareness campaigns may benefit from incorporating family health history information. Diabetes prevention programmes should address barriers beyond health beliefs to promote engagement in behaviour change.
Are dizziness/lightheadedness helpful symptoms for telephone triage of females and males calling the out-of-hours service for acute chest discomfort? A cross-sectional study
Authors: Dobbe Anna S M , Zwart Dorien L , van der Hem Laurens , Erkelens D Carmen , Wouters Loes T C M , van Doorn Sander , den Ruijter Hester M , Rutten Frans H 
Published: February 2025

ObjectivesSymptoms of dizziness/lightheadedness are more often reported by females than males with an acute coronary syndrome (ACS). Therefore, we investigated if these symptoms are helpful for triage and diagnosis of ACS in females and males with acute chest discomfort.DesignCross-sectional study.SettingNine out-of-hours service in primary care (OHS-PC) in the Netherlands participated, covering both rural and urban areas, serving a total population of 1.5 million people.Participants2195 patients were selected calling the OHS-PC with acute chest discomfort, 55.4% were females. Calls were selected based on International Classification of Primary Care codes and keywords, that is, chest pain, heart complaints, heart, myocardial infarction, heart attack, heart infarction or common abbreviations of these keywords. Recordings were excluded if (1) they were of poor quality, (2) the conversation was not a triage conversation, (3) patients were <18 years, (4) patients did not live in the vicinity of the OHS-PC, and (4) general practitioners did not want to provide follow-up information including the final diagnosis.Primary and secondary outcome measuresThe prevalence of dizziness/lightheadedness among patients calling the OHS-PC with acute chest discomfort and the relation between dizziness/lightheadedness and (1) urgency allocation and (2) a final diagnosis of ACS.ResultsAmong 2195 patients who called the OHS-PC with acute chest discomfort, 251 (11.4%) had an ACS (8.3% females, 15.3% males). Females more often reported concurrent dizziness/lightheadedness than males (14.7% vs 10.9%, p=0.008). However, this was not observed in those with confirmed ACS (9.9% of the females vs 9.3% of the males, p=0.881). Reporting of dizziness/lightheadedness was not positively related to an ACS diagnosis in females (OR 0.61 (95% CI 0.31 to 1.21)) or in males (OR 0.82 (95% CI 0.45 to 1.47)). In contrast, both females and males with concurrent dizziness/lightheadedness more often received a high urgency allocation than those without these symptoms.ConclusionsDizziness/lightheadedness are common symptoms but are not positively related to an ACS diagnosis in both females and males with acute chest discomfort. It seems not worthwhile to give females and males with acute chest discomfort and concurrent dizziness/lightheadedness a higher urgency allocation than those without these symptoms.Trial registration numberNTR7331.
Impact of the COVID-19 pandemic on the volume and diagnoses of patients admitted to the paediatric emergency department: a retrospective analysis from a third-level hospital in Northeastern Mexico
Authors: Blanco-Murillo Paulina , Estrada-Mendizabal Ricardo J , Madrazo-Aguirre Katheryne Paola , Ruelas-Martinez Manuel Guillermo , Escobedo-Berumen Lucia , Pineda-Magaña Rommy Elizabeth , Garcia-Lima Maria Guadalupe , Tamez-Rivera Oscar 
Published: February 2025

BackgroundDuring the COVID-19 pandemic, there were noticeable changes in the number of admissions to paediatric emergency departments (EDs) worldwide, as well as in the patient’s diagnoses. There is scarce evidence about the impact of the pandemic on paediatric EDs in Latin America and Mexico. This study aimed to analyse the changes in volume and discharge diagnoses of patients admitted to a third-level paediatric ED in northeastern Mexico before and during the COVID-19 pandemic.MethodsA retrospective observational study was carried out to compare the number of admissions and discharge diagnoses between two 3-month periods: December 2020–February 2021 (prepandemic) and December 2019–February 2020 (pandemic). Other variables, such as age, sex and comorbidities, were analysed. χ2and Mann-Whitney U tests were used depending on the examined variable.ResultsThere was a 44.1% decrease in the total volume of patients admitted to the paediatric ED during the analysed pandemic period. The percentage of infectious diseases decreased from 45.7% to 19.3% (p<0.001), and respiratory diseases from 4.3% to 1.9% (p<0.001), including asthma exacerbations (3.1%–1.5%, p=0.012). There was an increase in the percentage of patients admitted due to accidents (6.6%–14.7%, p<0.001).ConclusionsA significant decrease in the total volume of patients, especially those who were admitted due to infectious and respiratory diseases, was documented. Gaining deeper knowledge of the impact of global health emergencies on the use of healthcare services is crucial for an adequate planning of resource allocation in case of future pandemics.
Role of peripheral nerve stimulation and percutaneous cryoneurolysis in preventing chronic postsurgical pain
Authors: Finneran John J , Ilfeld Brian M 
Published: February 2025

BackgroundPoorly controlled pain during the acute postoperative period is associated with the development of persistent or ‘chronic’ pain lasting months or years after surgery. Relatively small trials suggest that local anesthetic-based peripheral nerve blocks lasting hours or a few days may decrease persistent postsurgical pain for some surgical procedures, but definitive data is lacking. Two possible alternatives—percutaneous cryoneurolysis and peripheral nerve stimulation—are analgesic modalities with the potential to provide weeks or months of pain relief following surgery. This increase in analgesic duration raises the possibility of decreased transition from acute to chronic postsurgical pain.ObjectiveThis review aims to summarize the available evidence involving the use of percutaneous cryoneurolysis and peripheral nerve stimulation within the immediate perioperative period and its effects on decreasing chronic postoperative pain.FindingsTwo randomized trials (n=66 and 16) comparing active percutaneous peripheral nerve stimulation to sham stimulation and two randomized trials (n=60 and 7) comparing percutaneous cryoneurolysis to a sham procedure for postoperative pain are described in this review. In each trial, participants were followed for at least three months.ConclusionThis review describes percutaneous cryoneurolysis and peripheral nerve stimulation for perioperative analgesia as well as the available evidence supporting their use to prevent persistent postsurgical pain.
In-situ shear modulus reduction with strain in stiff fissured clays and weathered mudstones
Authors: Briggs Kevin Mark , Trinidad González Yuderka , Meijer Gerrit Johannes , Powrie William , Butler Simon , Sartain Nick 
Published: February 2025

The non-linear stress-strain behaviour of stiff clays and weak rocks at small and medium strains may be a critical consideration in the design of geotechnical structures. Empirical methods have been developed for estimating the maximum shear modulus and the normalised shear modulus reduction with strain of fine-grained soils. These are usually expressed as functions of the void ratio (or specific volume) and average effective (confining) stress, based on results from laboratory tests. However, the fidelity of these equations has not been widely evaluated in-situ. This paper describes the use of in-situ measurements from an instrumented embankment to calculate the operational in-situ shear modulus of the underlying stiff clays and weathered mudstones at medium and large strains. It is shown that the shear modulus at very small strain of the weathered clays increased linearly with depth, consistent with empirical equations. The gradient of the normalised, non-linear stiffnesses of the clays were comparable with those measured in laboratory tests of fine-grained soils, at a range of strains. However, the values for the reference strain, where the maximum shear modulus reduces by 50%, were lower than was predicted by the empirical equations.