bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect ofSaccharomyces cerevisiaePostbiotics and Essential Oil on Growth Performance and Intestinal Health of Weanling Pigs During K88 ETEC Infection
Authors: Yan Hui , Xing Qian , Xiao Xiarui , Yu Bing , He Jun , Mao Xiangbing , Yu Jie , Zheng Ping , Luo Yuheng , Wu Aimin , Pu Junning , Lu Ping , Wei Ming , Khafipour Ehsan , Chen Daiwen 
Published: January 2024

AbstractEnterotoxigenic Escherichia coli (ETEC) is one of the major bacterial infections, causing substantial economic losses globally in the swine industry. This study aimed to investigate the impact of low Saccharomyces cerevisiae fermentation postbiotics (SCFP), high SCFP, essential oil (EO), or their combination on the growth performance and health of weanling pigs during ETEC infection. Forty-eight male weanling pigs were randomly allocated to five groups: 1) control group (CON—basal diet, n = 16); 2) low SCFP group (LSC—basal diet + 1.25 g/kg SCFP, n = 8); 3) high SCFP group (HSC—basal diet + 2 g/kg SCFP, n = 8); 4) essential oil group (EO—basal diet + 0.4 g/kg EO, n = 8); 5) the SCFP and EO combination group (SE—basal diet + 1.25 g/kg SCFP + 0.4 g/kg EO, n = 8). On day 15 of the trial, pigs in CON were divided into positive control (PC) and negative control (NC), and all pigs, except in NC, were challenged with ETEC. Under the normal condition, dietary LSC, HSC, EO, and EO all increased average daily gain (ADG) (P &lt; 0.05), and decreased F:G ratio (P &lt; 0.05) accompanied by decreased malondialdehyde (MDA) and increases in catalase (CAT), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) indicating enhanced anti-oxidative capacity, as well as decreased IL-2, IL-8, INF-γ, indicating mitigated systemic inflammation. During ETEC infection, all treatments alleviated ETEC-induced ADG reduction, diarrhea, damages in intestinal permeability and morphology, and down-regulation of tight junctions (Claudin1, ZO-1, and Occludin), while HSC and EO exhibited additional protections. All treatments increased CAT, T-SOD, and T-AOC, and decreased MDA in serum and jejunal mucosa at similar degrees (P &lt; 0.05). Moreover, all treatments alleviated ETEC-induced inflammation as shown by decreased IL-6, TNF-α, INF-γ, and increased IL-4 and IL-10 in serum or jejunal mucosa (P &lt; 0.05), and enhanced the immunity by increased serum IgG and mucosal sIgA (P &lt; 0.05). HSC and SE further reduced mucosal INF-γ and TNF-α than LSC or EO aligning with their additional protection against diarrhea during ETEC infection. Additionally, the key gut bacteria (e.g., Terrisporobacter) related to the benefits of SCFP and EO were identified. In sum, all treatments enhanced growth performance and protected against ETEC-induced intestinal damage through the regulation of redox and immune homeostasis. HSP and SE offered extra protection during disease for their additional control of inflammation. Our study provided new insight into the use of feed additives in the context of animal health states.
Phenylalanine requirements using the direct amino acid oxidation technique, and the effects of dietary phenylalanine on food intake, gastric emptying, and macronutrient metabolism in adult cats
Authors: Lambie Jocelyn G , Pezzali Júlia G , Richards Taylor L , Ellis Jennifer L , Verbrugghe Adronie , Shoveller Anna K 
Published: January 2024

Abstract
Despite Phe being an indispensable amino acid for cats, the minimum Phe requirement for adult cats has not been empirically defined. The objective of study 1 was to determine the minimum Phe requirement, where Tyr is in excess, in adult cats using the direct amino acid oxidation (DAAO) technique. Four adult male cats were used in an 8 × 4 Latin rectangle design. Cats were adapted to a basal diet for 7 d, top dressed with Phe to meet 140% of the adequate intake (NRC, 2006. Nutrient requirements of dogs and cats. Washington, DC: Natl. Acad. Press). Cats were randomly assigned to one of eight experimental Phe diets (0.29%, 0.34%, 0.39%, 0.44%, 0.54%, 0.64%, 0.74%, and 0.84% Phe in the diet on a dry matter [DM] basis). Following 1 d of diet adaptation, individual DAAO studies were performed. During each DAAO study, cats were placed into individual indirect calorimetry chambers, and 75% of the cat’s daily meal was divided into 13 equal meals supplied with a dose of L-[1-13C]-Phe. Oxidation of L-[1-13C]-Phe (F13CO2) during isotopic steady state was determined from the enrichment of 13CO2 in breath. Competing models were applied using the NLMIXED procedure in SAS to determine the effects of dietary Phe on 13CO2. The mean population minimum requirement for Phe was estimated at 0.32% DM and the upper 95% population confidence limit at 0.59% DM on an energy density of 4,200 kcal of metabolizable energy/kg DM calculated using the modified Atwater factors. In study 2, the effects of a bolus dose of Phe (44 mg kg−1 BW) on food intake, gastric emptying (GE), and macronutrient metabolism were assessed in a crossover design with 12 male cats. For food intake, cats were given Phe 15 min before 120% of their daily food was offered and food intake was measured. Treatment, day, and their interaction were evaluated using PROC GLIMMIX in SAS. Treatment did not affect any food intake parameters (P &gt; 0.05). For GE and macronutrient metabolism, cats were placed into individual indirect calorimetry chambers, received the same bolus dose of Phe, and 15 min later received 13C-octanoic acid (5 mg kg−1 BW) on 50% of their daily food intake. Breath samples were collected to measure 13CO2. The effect of treatment was evaluated using PROC GLIMMIX in SAS. Treatment did not affect total GE (P &gt; 0.05), but cats receiving Phe tended to delay time to peak enrichment (0.05 &lt; P ≤ 0.10). Overall, Phe at a bolus dose of 44 mg kg−1 BW had no effect on food intake, GE, or macronutrient metabolism. Together, these results suggest that the bolus dose of Phe used may not be sufficient to elicit a GE response, but a study with a greater number of cats and greater food intake is warranted.
Gift Giving in Enduring Dyadic Relationships: The Micropolitics of Mother–Daughter Gift Exchange
Authors: Liu Chihling , Kozinets Robert V , Patterson Anthony , Zhao Xin 
Published: January 2024

Abstract
This article investigates the dynamics of long-term gift exchange between British mothers and their adult daughters, delving into the processes behind dyadic gift giving. Through 54 comprehensive interviews, we elaborate the micropolitics that characterize these dynamics. Micropolitics refers to the subtle, everyday interactions, including gift exchange, that shape the ongoing negotiation of roles and the management of conflict or consensus within relationships. The study uncovers how these micropolitics manifest through four distinct processes of gift exchange: confirming, endorsing, connoting, and commanding. Gifts emerge as key instruments in this negotiation, serving as a medium for the reciprocal regulation of role behavior concerning gender, identity, and both endo-dyadic (within the dyad) and exo-dyadic (outside the dyad) roles. In contrast to previous research that adopts a synchronic (snapshot) approach to gift giving, our diachronic (over time) perspective emphasizes how power dynamics, intent, and identity politics evolve to sustain and transform relationships. Our findings illustrate the important communicative and power-laden processes of gift exchange in enduring relations, explaining why even unwanted gifts can have significant bonding value. Our study offers fresh perspectives on the continuous evolution of relationship and role dynamics, as viewed through the practices of gift exchange.
Descriptive Analysis of Community Based Needs among Asylum Seekers in the Greater Rhode Island Area before and after COVID-19: Evidence from a Student-Run Asylum Clinic
Authors: Angenent-Mari Victoria , Kasthuri Viknesh S , Montoya Hannah , Toll Elizabeth 
Published: January 2024

Abstract
The Brown Human Rights Asylum Clinic (BHRAC) is a medical-student-run asylum clinic which provides pro-bono medical and psychological affidavits for people in immigration proceedings. At the time of the evaluations for affidavits, a BHRAC student administers a Social Determinants of Health (SDoH) screener, evaluating clients for access to healthcare and social needs such as assistance with utilities payments and housing. Medical students follow up later and connect clients with trusted resources. This descriptive analysis reviews the population demographics; demonstrates common resource requests within our population before and after the start of COVID-19; and suggests possible improvements to our clinic model. Of clients who completed the SDoH screening, 89 per cent requested at least one form of assistance; their most common request was for mental health services, followed by English classes and medical services. No change to community resource request trends appeared before and after the start of COVID-19. Barriers encountered included delays and difficulty administering the survey to clients who did not speak English or Spanish, the ethical concern of the evaluation team also offering community resources for future care, and the changes resulting from annual rotation in BHRAC student leadership.
Dynamics of dimorphic workers of Constrictotermes cyphergaster (Blattodea: Termitidae) during nest repair
Authors: Andrade Marllon Rinaldo de Lima , Eloi Igor , de Oliveira Mário Herculano , Bezerra-Gusmão Maria Avany 
Published: January 2024

Abstract
Termite nest repairs are considered a defensive conduct as they reduce the colony’s exposure to the external environment. Repair activities are carried out by worker castes that can be polymorphic, representing a relationship between polymorphism and divisions of functions that can enhance task completion. Repairs are influenced by the extent of damage, nest volume, and the population dynamics of the building species, which regulate the recruitment of individuals for this activity. Our objective was to verify the performances (recruitment for repair) of dimorphic workers of Constrictotermes cyphergaster (Silvestri, 1901) during the damage repair activities performed on the external walls of termite nests of different sizes. We found a significant difference in the presence of dimorphic workers that performed repairs, with greater recruitment of the small morphotype, and observed an alternation of morphotypes between initial and final repair activities, with no influence of morphotype on the replacement pattern. Our results also showed that the total number of recruited workers decreased with increasing nest volume. These results help to better understand the social organization of a Nasutitermitinae termite species and the strategies adopted to protect its colonies.
Propionate functions as a feeding state–dependent regulatory metabolite to counter proinflammatory signaling linked to nutrient load and obesity
Authors: Han Kim , Meadows Allison M , Rodman Matthew J , Russo Anna Chiara , Sharma Rahul , Singh Komudi , Hassanzadeh Shahin , Dagur Pradeep K , Huffstutler Rebecca D , Krause Fynn N , Griffin Julian L , Baumer Yvonne , Powell-Wiley Tiffany M , Sack Michael N 
Published: January 2024

Abstract
Generally, fasting and refeeding confer anti- and proinflammatory effects, respectively. In humans, these caloric-load interventions function, in part, via regulation of CD4+ T cell biology. However, mechanisms orchestrating this regulation remain incomplete. We employed integrative bioinformatics of RNA sequencing and high-performance liquid chromatography–mass spectrometry data to measure serum metabolites and gene expression of peripheral blood mononuclear cells isolated from fasting and refeeding in volunteers to identify nutrient-load metabolite-driven immunoregulation. Propionate, a short chain fatty acid (SCFA), and the SCFA-sensing G protein–coupled receptor 43 (ffar2) were coordinately and inversely regulated by fasting and refeeding. Propionate and free fatty acid receptor agonists decreased interferon-γ and interleukin-17 and significantly blunted histone deacetylase activity in CD4+ T cells. Furthermore, propionate blunted nuclear factor κB activity and diminished interleukin-6 release. In parallel, propionate reduced phosphorylation of canonical T helper 1 (TH1) and TH17 regulators, STAT1 and STAT3, respectively. Conversely, knockdown of free fatty acid receptors significantly attenuated the anti-inflammatory role of propionate. Interestingly, propionate recapitulated the blunting of CD4+ TH cell activation in primary cells from obese individuals, extending the role of this metabolite to a disease associated with low-grade inflammation. Together, these data identify a nutrient-load responsive SCFA–G protein–coupled receptor linked pathway to regulate CD4+ TH cell immune responsiveness.
The Effective Use of Economics in the EU Digital Markets Act
Authors: Fletcher Amelia , Crémer Jacques , Heidhues Paul , Kimmelman Gene , Monti Giorgio , Podszun Rupprecht , Schnitzer Monika , Scott Morton Fiona , de Streel Alexandre 
Published: January 2024

Abstract
Economic thinking and analysis lie at the heart of the objectives and the design of the EU Digital Markets Act (DMA). However, the design of the DMA reflects a very deliberate—and reasonable—intention to ensure clarity, speed, administrability, and enforceability. In doing so, this pro-competitive regulation omits several elements of standard competition law where economics has typically played a key role. Nonetheless, we believe that economic insights and analysis—including behavioural economic thinking—will continue to play an important role in enabling the DMA to achieve its ambitious and laudable goals, albeit in a somewhat different way.
Natural products derived from herbs as promising source for diarrhea predominant-irritable bowel syndrome via gut microbiota–serotonin pathway
Authors: Zhang Mengmeng , Dang Ming , Li Yao , Wei PeiFeng , Zhao Chongbo , Dong Taiwei 
Published: January 2024

Abstract

Objective
Irritable bowel syndrome (IBS) is one of the most common gastrointestinal disorders, with diarrhea predominant IBS (IBS-D) as the most common subtype. Increasing evidence reported that the gut microbiota-mediated serotonin pathway plays a crucial role in the pathogenesis of IBS-D. In this study, potential herbal medicine, plant extracts and its monomers that can be employed as the candidate molecules for IBS-D through gut microbiota-mediated serotonin pathway were reviewed.

Key findings
The bacteria indigenous to gut microbiota regulates serotonin pathway, mainly increasing tryptophan hydroxylase (TPH) and decreasing serotonin reuptake transporter (SERT), by activating cyclooxygenase/prostaglandin E2 (COX/PGE2) signaling. It further accelerated gastrointestinal motility and visceral hyperalgesia. Herbal medicine prescription including Tongxie yaofang and Shugan decoction, as well as some monomers of flavonoid and polyphenol compounds can be regarded as the potential agents for IBS-D. The predominate mechanisms were related to regulating serotonin pathway by driving on the specific bacterial abundance (such as Firmicutes and Bacteroidetes). However, there are few reports on which specific bacteria species play a regulatory role in serotonin pathway, and most of these effective agents were only evidenced by preclinical studies. We hope this review will provide some useful directions for the treatment strategy of IBS-D.
Integrative conformal p-values for out-of-distribution testing with labelled outliers
Authors: Liang Ziyi , Sesia Matteo , Sun Wenguang 
Published: January 2024

Abstract
This paper presents a conformal inference method for out-of-distribution testing that leverages side information from labelled outliers, which are commonly underutilized or even discarded by conventional conformal p-values. This solution is practical and blends inductive and transductive inference strategies to adaptively weight conformal p-values, while also automatically leveraging the most powerful model from a collection of one-class and binary classifiers. Further, this approach leads to rigorous false discovery rate control in multiple testing when combined with a conditional calibration strategy. Extensive numerical simulations show that the proposed method outperforms existing approaches.
Chikungunya infection in returned travellers: results from the GeoSentinel network, 2005–2020
Authors: Bierbrier Rachel , Javelle Emilie , Norman Francesca F , Chen Lin Hwei , Bottieau Emmanuel , Schwartz Eli , Leder Karin , Angelo Kristina M , Stoney Rhett J , Libman Michael , Hamer Davidson H , Huits Ralph , Connor Bradley A , Simon Fabrice , Barkati Sapha ,   for the GeoSentinel Network
Published: January 2024

Abstract

Background
Chikungunya is an important travel-related disease because of its rapid geographical expansion and potential for prolonged morbidity. Improved understanding of the epidemiology of travel-related chikungunya infections may influence prevention strategies including education and vaccination.

Methods
We analysed data from travellers with confirmed or probable chikungunya reported to GeoSentinel sites from 2005 to 2020. Confirmed chikungunya was defined as a compatible clinical history plus either virus isolation, positive nucleic acid test or seroconversion/rising titre in paired sera. Probable chikungunya was defined as a compatible clinical history with a single positive serology result.

Results
1202 travellers (896 confirmed and 306 probable) with chikungunya were included. The median age was 43 years (range 0–91; interquartile range [IQR]: 31–55); 707 (58.8%) travellers were female. Most infections were acquired in the Caribbean (28.8%), Southeast Asia (22.8%), South Central Asia (14.2%) and South America (14.2%). The highest numbers of chikungunya cases reported to GeoSentinel were in 2014 (28.3%), 2015 (14.3%) and 2019 (11.9%). The most frequent reasons for travel were tourism (n = 592; 49.3%) and visiting friends or relatives (n = 334; 27.7%). The median time to presentation to a GeoSentinel site was 23 days (IQR: 7–52) after symptom onset. In travellers with confirmed chikungunya and no other reported illnesses, the most frequently reported symptoms included musculoskeletal symptoms (98.8%), fever/chills/sweats (68.7%) and dermatologic symptoms (35.5%). Among 917 travellers with information available, 296 (32.3%) had a pretravel consultation.

Conclusions
Chikungunya was acquired by international travellers in almost 100 destinations globally. Vector precautions and vaccination where recommended should be integrated into pretravel visits for travellers going to areas with chikungunya or areas with the potential for transmission. Continued surveillance of travel-related chikungunya may help public health officials and clinicians limit the transmission of this potentially debilitating disease by defining regions where protective measures (e.g. pretravel vaccination) should be strongly considered.
TARGET OF MONOPTEROS: key transcription factors orchestrating plant development and environmental response
Authors: Chen Min , Dai Yani , Liao Jiamin , Wu Huan , Lv Qiang , Huang Yu , Liu Lichang , Feng Yu , Lv Hongxuan , Zhou Bo , Peng Dan 
Published: January 2024

AbstractPlants have an incredible ability to sustain root and vascular growth after initiation of the embryonic root and the specification of vascular tissue in early embryos. Microarray assays have revealed that a group of transcription factors, TARGET OF MONOPTEROS (TMO), are important for embryonic root initiation in Arabidopsis. Despite the discovery of their auxin responsiveness early on, their function and mode of action remained unknown for many years. The advent of genome editing has accelerated the study of TMO transcription factors, revealing novel functions for biological processes such as vascular development, root system architecture, and response to environmental cues. This review covers recent achievements in understanding the developmental function and the genetic mode of action of TMO transcription factors in Arabidopsis and other plant species. We highlight the transcriptional and post-transcriptional regulation of TMO transcription factors in relation to their function, mainly in Arabidopsis. Finally, we provide suggestions for further research and potential applications in plant genetic engineering.
The CRISPR–Cas system in clinical strains of Acinetobacter baumannii: an in-silico analysis
Authors: Martínez-Trejo Arturo , Ruiz-Ruiz Juan Manuel , Gonzalez-Avila Luis Uriel , Saldaña-Padilla Andres , Hernández-Cortez Cecilia , de Jesús Colmenero-Solís Raúl , Bello-López Juan Manuel , Castro-Escarpulli Graciela 
Published: January 2024

Abstract
Acinetobacter baumannii is a relevant bacterium due to its high-resistance profile. It is well known that antimicrobial resistance is primarily linked to mutations and the acquisition of external genomic material, such as plasmids or phages, to which the Clustered Regularly Interspaced Short Palindromic Repeats associated with Cas proteins, or CRISPR–Cas, system is related. It is known that the system can influence the acquisition of foreign genetic material and play a role in various physiological pathways. In this study, we conducted an in-silico analysis using 91 fully assembled genomes of clinical strains obtained from the NCBI database. Among the analyzed genomes, the I-F1 subtype of the CRISPR–Cas system was detected showcasing variations in architecture and phylogeny. Using bioinformatic tools, we determined the presence, distribution, and specific characteristics of the CRISPR–Cas system. We found a possible association of the system with resistance genes but not with virulence determinants. Analysis of the system’s components, including spacer sequences, suggests its potential role in protecting against phage infections, highlighting its protective function.