bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep-underground search for the decay of $$^{180\textrm{m}}\text {Ta}$$ with an ultra-low-background HPGe detector
Authors: Cerroni R. , Dell’Oro S. , Formicola A. , Ghislandi S. , Ioannucci L. , Laubenstein M. , Lehnert B. , Nagorny S. S. , Nisi S. , Pagnanini L. 
Published: October 2023

Abstract$$^{180\textrm{m}}\text {Ta}$$

180
m

Ta

is the longest-lived metastable state presently known. Its decay has not been observed yet. In this work, we report a new result on the decay of $$^{180\textrm{m}}\text {Ta}$$

180
m

Ta

obtained with a 2015.12-g tantalum sample measured for 527.7 d with an ultra-low background HPGe detector in the STELLA laboratory of the Laboratori Nazionali del Gran Sasso (LNGS), in Italy. Before the measurement, the sample has been stored deep-underground for ten years, resulting in subdominant background contributions from cosmogenically activated $$^{182}\text {Ta}$$

182

Ta

. We observe no signal in the regions of interest and set half-life limits on the process for the two channels EC and $$\beta ^-$$

β
-

: $$T_{1/2,~\textrm{EC}}> 1.6 \times 10^{18}$$

T

1
/
2
,

EC

>
1.6
×

10
18

year and $$T_{1/2,~\beta ^-}> 1.1\times 10^{18}$$

T

1
/
2
,

β
-

>
1.1
×

10
18

year (90% C. I.), respectively. We also set the limit on the $$\gamma $$
γ
de-excitation / IC channel: $$T_{1/2,~\textrm{IC}}> 4.1 \times 10^{15}$$

T

1
/
2
,

IC

>
4.1
×

10
15

year (90% C. I.). These are, as of now, the most stringent bounds on the decay of $$^{180\textrm{m}}\text {Ta}$$

180
m

Ta

worldwide. Finally, we test the hypothetical scenarios of de-excitation of $$^{180\textrm{m}}\text {Ta}$$

180
m

Ta

by cosmological Dark Matter and constrain new parameter space for strongly-interacting dark-matter particle with mass up to $$10^5$$

10
5

GeV.
Genetic and Nongenetic Components of Stroke Family History: A Population Study of Adopted and Nonadopted Individuals
Authors: Mayerhofer Ernst , Parodi Livia , Narasimhalu Kaavya , Harloff Andreas , Georgakis Marios K. , Rosand Jonathan , Anderson Christopher D. 
Published: October 2023

Background
Genetic and nongenetic factors account for the association of family history with disease risk. Comparing adopted and nonadopted individuals provides an opportunity to disentangle those factors.

Methods and Results

We examined associations between family history of stroke and heart disease with incident stroke and myocardial infarction (MI) in 495 640 UK Biobank participants (mean age, 56.5 years; 55% women) stratified by childhood adoption status (5747 adoptees). We estimated hazard ratios (HRs) per affected family member, and for polygenic risk scores in Cox models adjusted for baseline age and sex. A total of 12 518 strokes and 23 923 MIs occurred over a 13‐year follow‐up. In nonadoptees, family history of stroke and heart disease was associated with increased stroke and MI risk, with the strongest association of family history of stroke for incident stroke (HR, 1.16 [95% CI, 1.12–1.19]) and family history of heart disease for incident MI (HR, 1.48 [95% CI, 1.45–1.50]). In adoptees, family history of stroke associated with incident stroke (HR, 1.41 [95% CI, 1.06–1.86]), but family history of heart disease was not associated with incident MI (
P
>0.5). Polygenic risk scores showed strong disease‐specific associations in both groups. In nonadoptees, the stroke polygenic risk score mediated 6% risk between family history of stroke and incident stroke, and the MI polygenic risk score mediated 13% risk between family history of heart disease and incident MI.

Conclusions
Family history of stroke and heart disease increases risk for their respective conditions. Family history of stroke contains substantial potentially modifiable nongenetic risk, indicating a need for novel prevention strategies, whereas family history of heart disease represents predominantly genetic risk.
Abstract GS.08: Cardiolipin Is A Novel Target Of Therapy To Mitigate Myocardial Injury Due To Veno-arterial Extracorporeal Membrane Oxygenation (va-ecmo)
Authors: Kapur Navin K , Swain Lija , Bhave Shreyas , Qiao Xiaoying , Reyelt Lara A , Mahmoudi Elena , Everett Kay , Warner Mary , Awata Junya , Chin Michael T 
Published: October 2023

Cardiolipin (CL) is a mitochondrial specific phospholipid that maintains integrity of the electron transport chain (ETC) and plays a central role in myocardial ischemia-reperfusion injury. VA-ECMO use to provide hemodynamic support for acute myocardial infarction (AMI) has grown exponentially; is associated with poor outcomes; and is the subject of active clinical trials, yet the mechanistic impact of VA-ECMO on myocardial damage in AMI remains poorly understood. We hypothesized that VA-ECMO acutely depletes myocardial CL and promotes myocardial damage in AMI.
We first report that levels of CL and tafazzin, a key enzyme required for CL synthesis, are significantly reduced in the left ventricles (LV) of human subjects requiring VA-ECMO compared to subjects without VA-ECMO prior to heart transplantation. Six hours of exposure to VA-ECMO also decreased LV levels of CL and tafazzin in healthy swine compared to sham controls. To explore whether CL depletion by VA-ECMO increases infarct size, we performed left anterior descending artery (LAD) occlusion for a total of 120 minutes followed by 180 minutes of reperfusion in adult swine in the presence and absence of MTP-131, an amphipathic molecule that interacts with CL to stabilize the inner mitochondrial membrane. Compared to reperfusion alone, VA-ECMO activation beginning after 90 minutes of LAD occlusion increased infarct size (33±11% vs 48±7%, p<0.001). VA-ECMO also decreased CL and tafazzin levels, disrupted mitochondrial structural integrity, reduced ETC function and promoted oxidative stress. Compared to reperfusion alone or VA-ECMO before reperfusion, intracoronary delivery of MTP-131 five minutes before VA-ECMO activation reduced infarct size (22±8%, p=0.03 vs IRI and p<0.001 vs VA-ECMO). Treatment with MTP-131 also restored CL and tafazzin levels, stabilized mitochondrial function, and reduced oxidative stress in the LV.
Our results identify a novel mechanism by which VA-ECMO promotes myocardial dysfunction and further identify cardiolipin as an important target of therapy to reduce infarct size and preserve mitochondrial function for patients requiring VA-ECMO for acute myocardial infarction. Future clinical studies are required to test the translational utility of this approach.
Abstract P1116: Histone Deacetylase Complexes Regulate Direct Cardiac Conversion From Human Fibroblasts
Authors: Wang Meimei , Lu Yu-An , Zhou Xinyue , Lu Rui , Zhou Yang 
Published: October 2023

Direct cardiac reprogramming is a promising approach to generate functional cardiomyocytes from non-myocytes for regenerative medicine, drug screening, and disease modeling. This method involves the transformation of specialized cells into functional cardiomyocytes using specific transcription factors or molecular signals. Epigenetic modulators, such as histone deacetylase (HDAC) complexes, may play a critical role in chromatin remodeling underlying cardiac reprogramming.
To investigate the role of HDAC complexes in direct cardiac conversion, induced cardiomyocyte-like cells (iCMs) were derived from human fibroblasts through ectopic expression of reprogramming factors, and the changes of histone acetylation were evaluated by western blotting. The results showed decreased levels of H3K9ac, H3K14ac, and H3K27ac along with direct cardiac reprogramming, suggesting the significant chromatin remodeling and gene regulation.
A loss-of-function screen of all the components associated with HDAC complex was performed to determine the responsible histone acetylation modifiers. The screen results showed that most of the HDAC components are necessary for direct cardiac reprogramming. Further investigation focused on a newly identified HDAC complex, mitotic deacetylase complex (MiDAC), consisting of mitotic deacetylase associated SANT domain protein MiDEAS (ELMSAN1), DNTTIP1, and HDAC1. Knocking down each of the MiDAC components led to similar reprogramming repression and cell death, indicating that MiDAC plays a critical role in cardiac reprogramming. In addition, the expression analysis of DNTTIP1 and HDAC1 showed a gradual decrease throughout the conversion, while ELMSAN1 diminished at the early stage of reprogramming, suggesting that ELMSAN1 is likely required for MiDAC stability. Furthermore, overexpression of ELMSAN1 significantly improved reprogramming efficiency and led to the increase of H3K9ac, H3K14ac, and H3K27ac levels globally, suggesting that ELMSAN1 and MiDAC might be responsible for the regulation of such histone modifications during the process.
Overall, our findings provide novel insights into the epigenetic regulation of direct cardiac reprogramming and reveal the potential role of MiDAC in this process.
Abstract P3001: Regulation Of Perivascular Adipose Tissue By Follistatin In Hypertension
Authors: Kuganathan Ann , Leal Marcos , Lu Vincent , MacDonald Melissa , Gao Bo , Dickhout Jeffrey , Krepinsky Joan 
Published: October 2023

Follistatin lowers BP and improves resistance artery health in spontaneously hypertensive rats (SHR), a model of essential HTN. Functionally, follistatin improves smooth muscle contraction and endothelium-dependent relaxation. Structurally, increased medial collagen was inhibited. Vascular ROS were also reduced. Perivascular adipose tissue (PVAT) regulates vascular tone via release of vasoactive substances. Oxidative stress in PVAT, as in HTN, induces release of contractile agents. Transition from white to brown PVAT is associated with decreased risk of CVD. We analyze effects of follistatin on PVAT and its regulation of vessel function in SHR and normotensive Wistar Kyoto (WKY) rats.

White and brown PVAT were dissected from treated rats; arteries were isolated from untreated donors. PVAT was introduced near strain-matched donor arteries and assessed for ROS

levels and contractility using DHE and wire myography, respectively. In some vessels, endothelium was denuded to assess its contribution to contraction.

Follistatin reduced ROS, hypercontractility, and improved relaxation in a PVAT-dependent manner. Brown PVAT induced significant anti-contractile and antioxidant effects. Elevated expression of adipose tissue browning markers and decreased adipocyte area with follistatin, support PVAT browning effects. Endothelium removal results in augmented vessel constriction, effects reduced by follistatin.
Follistatin improves SHR smooth muscle tone in a PVAT-dependent manner, likely through the inhibition of vascular ROS and browning of PVAT. Future work will profile PVAT effects of follistatin through unbiased proteomic analysis. Although most vessels are surrounded by PVAT, studies of PVAT-vessel interaction in HTN are few. By characterizing PVAT dysfunction, we aim to outline specific PVAT-mediated pathways involved in lowering BP and improving vascular function and structure. Results will provide new insights into PVAT-targeted therapy for essential HTN, allowing potential translation of benefits to obesity and other diseases exhibiting PVAT dysfunction.
Abstract P3046: The Effect Of MicroRNA-367-3p And MicroRNA-148a-3p On Vascular Endothelial Cells In Context Of Senescence And Infarct Healing
Authors: Hehl Laura , Kalies Katrin , Daniel Fiene , Knoepp Kai , Dutzmann Jochen , SEDDING Daniel , Köster Jan Michael , Guerlach Simon , Boettcher Solveig 
Published: October 2023

To improve the healing after myocardial infarction (MI), a well-coordinated angiogenic response is necessary. MicroRNAs (miRNAs) were identified as regulators of angiogenesis, however, strategies to improve angiogenesis by targeting miRNAs are challenging. Here, we evaluate miRNA-367-3p and 148a-3p as novel miRNA candidates regarding their therapeutic potential toward neovascularization after MI.
Following expression analysis of the respective miRNAs in human vascular endothelial cells (EC) and mice tissue, their contribution to EC function after either pre- or anti-miR transfection was assessed. Further, potential targets were identified and studied on mRNA and protein level.

Initial screenings show that miRNA-148a-3p and miRNA-367-3p are differently expressed in both a restenosis model via wire-induced injury and aged mice tissue (p<0.05). Under growth conditions, miRNA-148a-3p expression was upregulated in ECs. In senescent ECs, miRNA-367-3p shows a significant 50-fold upregulation of its expression (p<0.01); miRNA-148a-3p is also overexpressed in senescent ECs (p<0.01). Hypoxia increases the effect observed in senescence (p<0.05). Downregulation of either miRNA-367-3p or miRNA 148a-3p leads to an improvement in migration capacity (p<0.0001) and proliferation of ECs (p<0.0001). Systematic target research
in silico
identified DKK3 as potential target of miRNA-367-3p and DDX6 as potential target of miRNA-148a-3p. Downregulation of either miRNA-148a-3p or miRNA-367-3p verified these predictions by significantly upregulating DKK3 respectively DDX6. In further experiments, we study the dysfunctional effect of miRNA-148a-3p on vascular endothelial cells by targeting DDX6 as well as the anti-angiogenic impact of miRNA-367-3p by targeting DKK3.

Summarizing different expression levels of miRNA-367-3p and miRNA-148a-3p exerted distinct effects on EC function, especially in senescent cells and under hypoxic conditions. Our experimental data regarding cellular function show that these miRNAs are involved in the angiogenetic response and hold the potential to serve as therapeutic targets. In future experiments, we will evaluate the miRNA potential on MI healing
in vivo.
Bibliography of Women and Gender in China (2018-2022)
Authors: Yates Robin D.S. , Cai Danni 
Published: October 2023

Abstract
This bibliography is the third iteration of a bibliography of women and gender in China studies. The first was published in book form by Brill in 2009 and the second was published in this journal, Nan Nü: Men, Women and Gender in China, in 2018. It has been compiled in celebration of the twenty-fifth anniversary of the journal. It contains approximately 1200 citations of works published primarily since 2018 (with some items missed in the previous bibliographies) in the form of books, chapters in books, or journal articles, with some items published only in online journals. The articles have been culled from approximately 200 journals. Most of the items were published in English in multiple disciplines. The index lists all the authors in 18 categories, some of whom appear in more than one category.
Zeolitic imidazolate framework-8 modified bacterial cellulose/sodium alginate composite aerogel for efficient removal of tetracycline hydrochloride
Authors: Yang Xu , Bismark Sarkodie , Wang Dengbing , Liu Zuyi , Tao Yun , Sun Ziwei , Han Xu , Feng Quan 
Published: October 2023

Antibiotics released in the water pose a serious threat to human and ecological health. Therefore, it is of great importance to effectively remove antibiotics from wastewater. In this study, recyclable bacterial cellulose/sodium alginate/zeolitic imidazolate framework-8 (BC/SA/ZIF-8) aerogel composites were prepared by chemical cross-linking and in situ growth in freeze-drying. The successful preparation of the composite aerogel was confirmed by scanning electron microscope (SEM), Fourier Transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. The results reveal that BC/SA/ZIF-8 aerogel composite has significantly high BET specific surface area of 165.24 m2/g than that of BC/SA at 7.39 m2/g. The BC/SA/ZIF-8 composite aerogel exhibited excellent adsorption performance for tetracycline hydrochloride, with a maximum adsorption capacity of 746.27 mg/g. The adsorption process followed the pseudo-second-order kinetic model and Langmuir adsorption isotherm model. Moreover, the reusability test on BC/SA/ZIF-8 composite aerogel revealed an insignificant decline in adsorption after six cycles. Therefore, BC/SA/ZIF-8 composite aerogel has a high potential as a novel and recyclable adsorbent for efficient removal of tetracycline hydrochloride from water.
The relationship between the perceived risk of infection at the workplace and quality of work life (QoWL): Healthcare workers’ perspectives
Authors: Alolayyan Main Naser , Alyahya Mohammad , Momani Salam 
Published: October 2023

Background One of today’s most significant organizational priorities, including healthcare organizations, is to improve the quality of work-life (QoWL), which leads to offering high-quality services to patients. Aim The study aimed to explore the effect of the perceived risk of infection and its impact on the quality of work-life (QoWL) on HCWs during the COVID-19 pandemic. Methods A cross-sectional survey was conducted on 484 healthcare workers. Respondents were recruited using convenience sampling methods. Results : The study findings revealed a better QoWL with a decreased perceived risk of COVID-19 infection. Conclusion Increasing the quality of work life will be reflected in adherence to health protocols, which creates a work environment capable of dealing with challenges, including the risk of infection such as COVID-19.
A survey of Israeli physical therapists regarding reactive balance training
Authors: Margalit Noam , Kurz Ilan , Wacht Oren , Mansfield Avril , Melzer Itshak 
Published: October 2023

Abstract
Background
‘Reactive balance training’ (RBT) was developed to improve balance reactions to unexpected losses of balance. Although this training method is effective, its practical usage in the field of physical-therapy in Israel and world-wide is still unclear.

Aims
This study aimed to evaluate the extent of RBT use in physical-therapy clinics in Israel, to identify the significant barriers to/facilitators for implementing RBT in clinical practice among physical therapists, and to determine which aspects of RBT most interest physical therapists in Israel.

Methods
Physical therapists in Israel completed a survey using a questionnaire regarding their knowledge and use of RBT in their clinical practices. We compared the specific use of RBT among users; non-users; and open-to-use physical therapists. The odds ratios of the facilitators and barriers were calculated using univariate and multivariate logistic regression models.

Results
Four-hundred and two physical therapists responded to a yes/no question regarding their use of RBT. Three-quarters (75.4%) of physical therapists reported using RBT in their practices. The most prevalent barrier cited was insufficient space for setting up equipment and most prevalent facilitator was having a colleague who uses RBT. Most of the respondents wanted to learn more about RBT, and most of the non-users wanted to expand their knowledge and mastery of RBT principles.

Conclusions
There are misconceptions and insufficient knowledge about RBT among physical therapists in Israel, indicating that they may falsely believe that RBT requires large and expensive equipment, suggesting they categorize RBT as external perturbation training only. Reliable information may help to improve general knowledge regarding RBT, and to facilitate the more widespread implementation of RBT as an effective fall-prevention intervention method.
CDC6, a key replication licensing factor, is overexpressed and confers poor prognosis in diffuse large B-cell lymphoma
Authors: Shen Mingfang , Zhang Yunfeng , Tang Lun , Fu Qinyan , Zhang Jiawei , Xu Yang , Zeng Hui , Li Yuan 
Published: October 2023

Abstract
Background
Cell division cycle 6 (CDC6) is a key licensing factor in the assembly of pre-replicative complexes at origins of replication. The role of CDC6 in the pathogenesis of in diffuse larger B-cell lymphoma (DLBCL) remains unknown. We aim to investigate the effects of CDC6 on the proliferation, apoptosis and cell cycle regulation in DLBCL cells, delineate its underlying mechanism, and to correlate CDC6 expression with clinical characteristics and prognosis of patients with DLBCL.

Methods
Initial bioinformatic analysis was performed to screen the potential role of CDC6 in DLBCL. Lentiviral constructs harboring CDC6 or shCDC6 was transfected to overexpress or knockdown CDC6 in SUDHL4 and OCI-LY7 cells. The cell proliferation was evaluated by CCK-8 assay, cell apoptosis was detected by Annexin-V APC/7-AAD double staining, and cell cycle was measured by flow cytometry. Real time quantitative PCR and western blot was used to characterize CDC6 expression and its downstream signaling pathways. The clinical data of DLBCL patients were retrospectively reviewed, the CDC6 expression in DLBCL or lymph node reactive hyperplasia tissues was evaluated by immunohistochemistry.

Results
In silico data suggest that CDC6 overexpression is associated with inferior prognosis of DLBCL. We found that CDC6 overexpression increased SUDHL4 or OCI-LY7 cell proliferation, while knockdown of CDC6 inhibited cell proliferation in a time-dependent manner. Upon overexpression, CDC6 reduced cells in G1 phase and did not affect cell apoptosis; CDC6 knockdown led to significant cell cycle arrest in G1 phase and increase in cell apoptosis. Western blot showed that CDC6 inhibited the expression of INK4, E-Cadherin and ATR, accompanied by increased Bcl-2 and deceased Bax expression. The CDC6 protein was overexpressed DLBCL compared with lymph node reactive hyperplasia, and CDC6 overexpression was associated with non-GCB subtype, and conferred poor PFS and OS in patients with DLBCL.

Conclusion
CDC6 promotes cell proliferation and survival of DLBCL cells through regulation of G1/S cell cycle checkpoint and apoptosis. CDC6 is overexpressed and serves as a novel prognostic marker in DLBCL.
Visual acuity assessment of central retinal artery occlusion patients with or without paracentral acute middle maculopathy via OCT-A
Authors: Gong Hongxia , Wu Bin , Xie Shiyong 
Published: October 2023

Abstract
Purpose
The association between paracentral acute middle maculopathy (PAMM) and visual acuity in patients with central retinal artery occlusion (CRAO) is still unclear. The present study investigated the visual acuity of CRAO patients with and without PAMM.

Methods
CRAO patients with PAMM or without PAMM were included. Optical coherence tomography angiography (OCT-A) was used to record the macular retinal thickness and density of shallow and deep vessels. The Best-corrected visual acuity (BCVA) was converted to a logarithm of the minimum angle of resolution (LogMAR) for statistical analysis.

Results
There were 34 CRAO patients with PAMM (43.13%), other 30 CRAO patients without PAMM (46.87%). Compared with the no-PAMM group, PAMM group had better LogMAR BCVA (1.48 (0.49, 1.85) Vs. 1.85 (1.70, 1.96), P < 0.01). There was also a significant difference in retinal thickness of the central macular sulcus (328.00 (304.50–332.25) Vs. 352.50 (311.75–420.50), P = 0.01). A significant correlation between LogMAR BCVA and macular retinal thickness was found (r = 0.42; P < 0.01).

Conclusion
CRAO patients with PAMM had significantly better visual acuity and less macular edema. OCT-A can be used to distinguish different levels of damage due to CRAO.