bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expansion Grade and Morphokinetic Parameters Associated with Aneuploidy Status of Embryo Fifth Day
Authors: Harzif Achmad K. , Natadisastra Muharam , Suryandari Dwi A. , Werdhani Retno A. , Jatmiko Wisnu , Budiono Arief , Bayuaji Hartanto , Wiweko Budi 
Published: December 2023

Objective: To determine the potential of examining embryo morphology, and morphokinetic parameters in predicting the chromosomal status of embryos.
Methods: This study is a cross-sectional that requires patients undergoing IVF followed by chromosome examination with NGS that was conducted at the IVF Center at Pondok Indah Hospital and Morula IVF Center at Bunda Hospital from December 2021 to December 2022. Each embryo that reaches the blastocyst stage on day 5 or 6 will be washed and put into a PCR tube for a week; then, embryologists annotate them to determine morphological assessment and morphokinetic parameters using Time-Lapse Microscopy. The chi-square test was used to analyse bivariate variables.
Results: One hundred twenty-four samples were collected on day 5 of patients undergoing the IVF procedure. 50.8% of the samples were aneuploid chromosomes, and 49.2% were euploid. The morphokinetic characteristics median was 3.86 fold. It was found that expansion grade, time to pro-nuclear fading, and time to the synchrony of the third cell cycle were significantly associated with euploid status (p = 0.000; 0.041 and 0.036).
Conclusion: The expansion grade has been proven as the most influential component for accurately predicting the ploidy status of embryos.
Keyword: blastocyst, embryo, euploid status, expansion grade, morphokinetics

Abstrak
Tujuan: Untuk mengetahui potensi pemeriksaan morfologi embrio, dan parameter morfokinetik dalam memprediksi status kromosom embrio.
Metode: Penelitian ini merupakan penelitian potong lintang yang memerlukan pasien yang sedang menjalani program bayi tabung yang dilanjutkan dengan pemeriksaan kromosom dengan NGS yang dilakukan di Pusat IVF RS Pondok Indah dan Pusat IVF Morula RS Bunda pada bulan Desember 2021 hingga Desember 2022. Setiap embrio yang mencapai tahap blastokista pada hari ke 5 atau 6 akan dicuci dan dimasukkan ke dalam tabung PCR selama seminggu; kemudian, ahli embriologi membuat anotasi untuk menentukan penilaian morfologi dan parameter morfokinetik menggunakan Mikroskop Time-Lapse. Uji chi-square digunakan untuk menganalisis variabel bivariat.
Hasil: Seratus dua puluh empat sampel dikumpulkan pada hari ke 5 dari pasien yang menjalani prosedur IVF. 50,8% sampel adalah kromosom aneuploid, dan 49,2% adalah euploid. Median karakteristik morfokinetik sebesar 3,86 kali lipat. Ditemukan bahwa tingkat ekspansi, waktu menuju pemudaran pro-nuklir, dan waktu hingga sinkronisasi siklus sel ketiga berhubungan secara signifikan dengan status euploid (p = 0,000; 0,041 dan 0,036).
Kesimpulan: Tingkat ekspansi telah terbukti sebagai komponen yang paling berpengaruh dalam memprediksi status ploidi embrio secara akurat.
Kata kunci: blastosist, embrio, status euploid, tingkat ekspansi, morfokinetik
Коморбідна ендокринна патологія як наслідок функціонально-метаболічних порушень печінки та її корекція новим засобом
Authors: Морозюк А. Ю. , Мельниківська Н. В. , Кудря М. Я. , Устенко Н. В. , Кустова С. П. , Бойко М. О. , Ганненко С. Г. , Матвєєва Т. В. 
Published: December 2023

У медичній практиці все більше поширюється таке явище, як коморбідність, тобто, існування в пацієнта двох або більше захворювань і синдромів, що пов’язані між собою, співпадаючих за часом, або тих, що є наслідком основного захворювання та його лікування. Оскільки печінка є одним з найважливіших метаболічних органів, розповсюдження захворювань печінки значно збільшує кількість пов’язаних з ними коморбідних патологій. Однією з поширених патологій гепатобіліарної системи в світі вважають неалкогольну жирову хворобу печінки, розвиток якої може призвести також до пору­шень з боку щитовидної залози, сперматогенезу тощо. Слід зазначити, що діагностика та лікування коморбідних патологій значно ускладнені через неспецифічні симптоми хвороби. Для ефективної корекції коморбідної патології, що поєднує порушення сперматогенезу з розвитком гіпотиреозу на фоні гепатиту, є перспективною розробка комбінованих лікарських засобів, які б поєднували спер­ матостимулюючу активність з гепатопротекторною та відновлюючим впливом на тиреоїдний статус організму. У ДУ «Інститут проблем ендокринної патології ім. В. Я. Данилевського НАМН України» розроблено такий інноваційний засіб на основі камфорної кислоти та селеновмісної сполуки.
Мета дослідження – оцінити вплив нового засобу на основі дикарбонової кислоти та селеновміс­ної сполуки на процеси сперматогенезу, тиреоїдний статус за умов токсичного та медикаментозно­го гепатиту.
Дослідження проведено на 80 безпородних білих щурах­самцях масою 200–250 г. Відтворено дві моделі експериментального гепатиту, токсичний (ураження тетрахлорметаном) і медикаментозний (парацетамол­індукований). Верифікацію ушкодження печінки проводили біохімічними методами: визначали активність АлАТ, АсАТ, ГГТ та ЛФ, також досліджували процеси сперматогенезу та рівень гормонів щитовидної залози.
В умовах як токсичного, так і медикаментозного ураження печінки в тварин виявлені значні зміни активності печінкових ферментів. Також відмічено суттеве зниження рівня тиреоїдних гормонів і значні порушення процесів сперматогенезу. В умовах корекції обох форм індукованого гепатиту новим засобом відмічена нормалізація активності печінкових ферментів, підвищення рівня тиреоїд­них гормонів у піддослідних тварин і нормалізація показників спермограми.
Отже, новий засіб на основі похідного дикарбонової кислоти та селеновмісної сполуки є ефектив­ним і перспективним як потенційний лікарський засіб для корекції коморбідної патології, що виникає внаслідок функціонально-­метаболічних порушень печінки.
An investigation of tendon strains in jersey finger injury load cases using a finite element neuromuscular human body model
Authors: Nölle Lennart V. , Alfaro Eduardo Herrera , Martynenko Oleksandr V. , Schmitt Syn 
Published: December 2023

Introduction: A common hand injury in American football, rugby and basketball is the so-called jersey finger injury (JFI), in which an eccentric overextension of the distal interphalangeal joint leads to an avulsion of the connected musculus flexor digitorum profundus (FDP) tendon. In the field of automotive safety assessment, finite element (FE) neuromuscular human body models (NHBMs) have been validated and are employed to evaluate different injury types related to car crash scenarios. The goal of this study is to show, how such a model can be modified to assess JFIs by adapting the hand of an FE-NHBM for the computational analysis of tendon strains during a generalized JFI load case.Methods: A jersey finger injury criterion (JFIC) covering the injury mechanisms of tendon straining and avulsion was defined based on biomechanical experiments found in the literature. The hand of the Total Human Model for Safety (THUMS) version 3.0 was combined with the musculature of THUMS version 5.03 to create a model with appropriate finger mobility. Muscle routing paths of FDP and musculus flexor digitorum superficialis (FDS) as well as tendon material parameters were optimized using literature data. A simplified JFI load case was simulated as the gripping of a cylindrical rod with finger flexor activation levels between 0% and 100%, which was then retracted with the velocity of a sprinting college football player to forcefully open the closed hand.Results: The optimization of the muscle routing node positions and tendon material parameters yielded good results with minimum normalized mean absolute error values of 0.79% and 7.16% respectively. Tendon avulsion injuries were detected in the middle and little finger for muscle activation levels of 80% and above, while no tendon or muscle strain injuries of any kind occurred.Discussion: The presented work outlines the steps necessary to adapt the hand model of a FE-NHBM for the assessment of JFIs using a newly defined injury criterion called the JFIC. The injury assessment results are in good agreement with documented JFI symptoms. At the same time, the need to rethink commonly asserted paradigms concerning the choice of muscle material parameters is highlighted.
TIMP-1 in the prognosis of patients who underwent coronary artery bypass surgery: a 12-year follow-up study
Authors: Huang Jui-Tzu , Sung Shih-Hsien , Hsu Chiao-Po , Chiang Chern-En , Yu Wen-Chung , Cheng Hao-Min , Huang Cheng-Hsiung 
Published: December 2023

IntroductionMatrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) have been linked to clinical outcomes in patients with coronary artery disease (CAD). However, the prognostic value of TIMP-1 in patients with CAD who underwent coronary artery bypass grafting (CABG) has not been elucidated. We aimed to investigate the correlations of TIMP-1 with high-sensitivity C-reactive protein (hs-CRP) and N-terminal pro-brain natriuretic peptide (NT-proBNP) in the long-term prognosis of consecutive patients who underwent CABG.MethodsA total of 234 patients (age: 70.4 ± 10.5 years, 84.6% men) with CAD who underwent CABG were prospectively enrolled. Preoperative levels of MMPs, TIMP-1, hs-CRP, and NT-proBNP were recorded. Major adverse cardiovascular events (MACE) were defined as non-fatal myocardial infarction, non-fatal stroke, and cardiovascular death.ResultsDuring a median follow-up of 12.1 years, 120 deaths were recorded. The deceased were older, had more manifest acute coronary syndrome (ACS), a lower left ventricular ejection fraction (LVEF), and an estimated glomerular filtration rate (eGFR), but significantly higher MMP13, TIMP-1, hs-CRP, and NT-proBNP compared with the survivors. After adjusting for age, sex, manifest ACS, eGFR, LVEF, total cholesterol, and triglycerides, TIMP-1 (hazard ratio and 95% confidence intervals per SD: 1.506, 1.183–1.917), hs-CRP (1.349, 1.183–1.561), and NT-ProBNP (1.707, 1.326–2.199) were all independently associated with all-cause mortality. The mediation analysis revealed that the mortality risks of TIMP-1 were partially mediated by NT-proBNP (62.2%) and hs-CRP (25.3%). The associations of TIMP-1 with MACE were partially mediated by NT-proBNP (54.4%) but not hs-CRP.ConclusionsTIMP-1 was an independent predictor of long-term outcomes after CABG, with possible roles in subclinical inflammation and postoperative cardiac remodeling.
The feasibility of relaxation-enhanced angiography without contrast and triggering for preprocedural planning of transcatheter aortic valve implantation
Authors: Wang Rui , Liu Xinmin , Yao Jing , Schoepf U. Joseph , Griffith Joseph , Wang Jiayang , Lian Jianxiu , Jiang Ke , Song Guangyuan , Xu Lei 
Published: December 2023

BackgroundCardiovascular MRI is advantageous in transcatheter aortic valve implantation (TAVI) planning. This study aimed to evaluate the feasibility of comprehensive non-contrast MRI [relaxation-enhanced angiography without contrast and triggering (REACT)] combined with a three-dimensional whole-heart MRI protocol for preprocedural planning of TAVI vs. computed tomography angiography (CTA).MethodsThirty patients with severe aortic stenosis were prospectively enrolled. The anatomical properties of the aortic root anatomy, including the perimeter and area of the virtual aortic valve annulus and coronary heights, were determined from 3D whole-heart MRI and cardiac CTA (CCTA) images, respectively. The diameters of the aorta (thoracic and abdominal aorta) and iliofemoral arteries were measured from REACT and aortic CTA (ACTA) images, respectively. A paired t-test was used to compare these two modalities. Bland–Altman plots were used to assess cardiovascular MRI and CTA measurements. Transcatheter heart valve (THV) sizing was performed based on CCTA measurements and compared with 3D whole-heart MRI measurements. The extent of annular calcification on 3D whole-heart MRI images was evaluated by a four-point grading scale and compared with CCTA data.ResultsAll 30 patients completed CTA and cardiovascular MRI examinations, with the TAVI procedure being administered in 25 patients. The mean acquisition time of the comprehensive MRI protocol was 18 ± 3.2 min. There were no significant differences between ACTA and REACT data in regard to the diameters of aortic and iliofemoral arteries, including the ascending thoracic aorta (37 ± 4.6 mm vs. 37.7 ± 5.2 mm, p = 0.085), descending thoracic aorta (24.3 ± 2.8 mm vs. 24.3 ± 2.8 mm, p = 0.832), abdominal aorta (20.9 ± 2.5 mm vs. 20.8 ± 2.5 mm, p = 0.602), bilateral common iliac arteries (right: 8.36 ± 1.44 mm vs. 8.42 ± 1.27 mm, p = 0.590; left: 8.61 ± 1.71 mm vs. 8.86 ± 1.46 mm, p = 0.050), and bilateral femoral arteries (right: 6.77 ± 1.06 mm vs. 6.87 ± 1.00 mm, p = 0.157; left: 6.75 ± 1.02 mm vs. 6.90 ± 0.80 mm, p = 0.142). Both modalities showed similar aortic valve morphology and semi-quantitative valve calcification (all, p's &gt; 0.05). Overall agreement for implanted THV was found in all 25 (100%) patients assessed with both modalities.ConclusionREACT combined with 3D whole-heart MRI enables reliable measurements of aortic root anatomy, annular calcification, and aorta and iliofemoral access in patients under evaluation for TAVI.
Characteristics and prognosis of isolated aortic valve infective endocarditis in patients with bicuspid aortic valves: a propensity matched study
Authors: Bohbot Yohann , Denev Seyhan , Benvenga Rossella M. , Philip Mary , Michelena Hector I. , Citro Rodolfo , Habib Gilbert , Tribouilloy Christophe 
Published: December 2023

IntroductionBicuspid aortic valve (BAV) is the most common congenital heart disease with an increased risk of infective endocarditis (IE). Few data are available on isolated native BAV-IE. The aim of this study was to compare patients with tricuspid aortic valve (TAV) IE and BAV-IE in terms of characteristics, management and prognosis.Material and methodsWe included 728 consecutive patients with IE on isolated native aortic valve in 3 centres: Amiens and Marseille Hospitals in France and Salerno Hospital in Italy. We studied in hospital and long-term mortality before and after matching for age, sex and comorbidity index. Median follow-up was 67.2 [IQR: 19–120] months.ResultsOf the 728 patients, 123 (16.9%) had BAV. Compared with patients with TAV-IE, patients with BAV-IE were younger, had fewer co-morbidities and were more likely to be male. They presented more major neurological events and perivalvular complications (both p &lt; 0.05). Early surgery (&lt;30 days) was performed in 52% of BAV-IE cases vs. 42.8% for TAV-IE (p = 0.061). The 10-year survival rate was 74 ± 5% in BAV-IE patients compared with 66 ± 2% in TAV-IE patients (p = 0.047). After propensity score matching (for age, gender and comorbidities), there was no difference in mortality between the two groups, with an estimated 10-year survival of 73 ± 5% vs. 76 ± 4% respectively (p = 0.91).ConclusionBAV is a frequent finding in patients with isolated aortic valve IE and is associated with more perivalvular complications and neurological events. The differences in survival with TAV-IE are probably related to the age and comorbidity differences between these two populations.
Do short-term, reduced-volume methods accurately reflect chronic toxic effects in the cladoceran Ceriodaphnia dubia? A study with the reference toxicant hexavalent chromium
Authors: Martínez-Jerónimo Fernando , Martínez-Jerónimo Laura 
Published: December 2023

Cladocerans are frequently used as test organisms in aquatic toxicology studies. For practical reasons (bioethical and economic), efforts are currently made in search of alternative animal models and in defining short-lasting test methods that will allow reliable evaluation of the chronic effect of chemical contaminants. The use of small-sized invertebrates, like the Ceriodaphnia dubia cladoceran, represents a good option and has been included in diverse testing protocols; however, the use of reduced volumes and shortened exposure times, as well as higher temperatures to accelerate their development could influence the results and lead to imprecise conclusions. The present study aimed to evaluate the effects of different test volumes, temperature, and exposure time on the toxicity of hexavalent chrome in C. dubia. In acute and chronic assays, three test volumes (5, 15, and 25 mL) were used. Once the LC50 was determined, four sublethal concentrations (0.01, 0.02, 0.04, and 0.08 mg L−1) of Cr(VI) were applied daily to each of the three test volumes for 21 days, evaluating the progeny and survival of adults. The LC50 values did not differ at 20°C, but at 25°C they were significantly lower in 5 mL. The chronic toxic effects varied depending on the test volume and exposure time. Significant effects were observed on day 7 of exposure (corresponding to the third reproduction) in the accumulated progeny only in the volume of 5 mL. Based on the aforementioned, it is necessary to revise whether the short-term and small-scale methods are not underestimating the detection of chronic effects.
Sex-related difference in the retinal structure of young adults: a machine learning approach
Authors: Farias Flávia Monteiro , Salomão Railson Cruz , Rocha Santos Enzo Gabriel , Sousa Caires Andrew , Sampaio Gabriela Santos Alvarez , Rosa Alexandre Antônio Marques , Costa Marcelo Fernandes , Silva Souza Givago 
Published: December 2023

PurposeTo compare the accuracy of machine learning (ML) algorithms to classify the sex of the participant from retinal thickness datasets in different retinal layers.MethodsThis cross-sectional study involved 26 male and 38 female subjects. Data were acquired using HRA + OCT Spectralis, and the thickness and volume of 10 retinal layers were quantified. A total of 10 features were extracted from each retinal layer. The accuracy of various algorithms, including k-nearest-neighbor, support vector classifier, logistic regression, linear discriminant analysis, random forest, decision tree, and Gaussian Naïve Bayes, was quantified. A two-way ANOVA was conducted to assess the ML accuracy, considering both the classifier type and the retinal layer as factors.ResultsA comparison of the accuracies achieved by various algorithms in classifying participant sex revealed superior results in datasets related to total retinal thickness and the retinal nerve fiber layer. In these instances, no significant differences in algorithm performance were observed (p &gt; 0.05). Conversely, in other layers, a decrease in classification accuracy was noted as the layer moved outward in the retina. Here, the random forest (RF) algorithm demonstrated superior performance compared to the others (p &lt; 0.05).ConclusionThe current research highlights the distinctive potential of various retinal layers in sex classification. Different layers and ML algorithms yield distinct accuracies. The RF algorithm’s consistent superiority suggests its effectiveness in identifying sex-related features from a range of retinal layers.
A long-term ketogenic diet in young and aged rats has dissociable effects on prelimbic cortex and CA3 ensemble activity
Authors: Hernandez Abbi R. , Barrett Maya E. , Lubke Katelyn N. , Maurer Andrew P. , Burke Sara N. 
Published: December 2023

IntroductionAge-related cognitive decline has been linked to distinct patterns of cellular dysfunction in the prelimbic cortex (PL) and the CA3 subregion of the hippocampus. Because higher cognitive functions require both structures, selectively targeting a neurobiological change in one region, at the expense of the other, is not likely to restore normal behavior in older animals. One change with age that both the PL and CA3 share, however, is a reduced ability to utilize glucose, which can produce aberrant neural activity patterns.MethodsThe current study used a ketogenic diet (KD) intervention, which reduces the brain’s reliance on glucose, and has been shown to improve cognition, as a metabolic treatment for restoring neural ensemble dynamics in aged rats. Expression of the immediate-early genes Arc and Homer1a were used to quantify the neural ensembles that were active in the home cage prior to behavior, during a working memory/biconditional association task, and a continuous spatial alternation task.ResultsAged rats on the control diet had increased activity in CA3 and less ensemble overlap in PL between different task conditions than did the young animals. In the PL, the KD was associated with increased activation of neurons in the superficial cortical layers, establishing a clear link between dietary macronutrient content and frontal cortical activity. The KD did not lead to any significant changes in CA3 activity.DiscussionThese observations suggest that the availability of ketone bodies may permit the engagement of compensatory mechanisms in the frontal cortices that produce better cognitive outcomes.
Increase of glutamate in satellite glial cells of the trigeminal ganglion in a rat model of craniofacial neuropathic pain
Authors: Cho Yi Sul , Mah Won , Youn Dong Ho , Kim Yu Shin , Ko Hyoung-Gon , Bae Jin Young , Kim Yun Sook , Bae Yong Chul 
Published: December 2023

IntroductionSatellite glial cells (SGCs) that envelop the cell bodies of neurons in sensory ganglia have been shown to both release glutamate, and be activated by glutamate in the context of nociceptive signaling. However, little is known about the subpopulations of SGCs that are activated following nerve injury and whether glutamate mechanisms in the SGCs are involved in the pathologic pain.MethodsTo address this issue, we used light and electron microscopic immunohistochemistry to examine the change in the glutamate levels in the SGCs and the structural relationship between neighboring neurons in the trigeminal ganglion (TG) in a rat model of craniofacial neuropathic pain, CCI-ION.ResultsAdministration of ionomycin, ATP and Bz-ATP induced an increase of extracellular glutamate concentration in cultured trigeminal SGCs, indicating a release of glutamate from SGCs. The level of glutamate immunostaining in the SGCs that envelop neurons of all sizes in the TG was significantly higher in rats with CCI-ION than in control rats, suggesting that SGCs enveloping nociceptive as well as non-nociceptive mechanosensitive neurons are activated following nerve injury, and that the glutamate release from SGCs increases in pathologic pain state. Close appositions between substance-P (SP)-immunopositive (+) or calcitonin gene-related peptide (CGRP)+, likely nociceptive neurons, between Piezo1+, likely non-nociceptive, mechanosensitive neurons and SP+ or CGRP+ neurons, and between SGCs of neighboring neurons were frequently observed.DiscussionThese findings suggest that glutamate in the trigeminal SGCs that envelop all types of neurons may play a role in the mechanisms of neuropathic pain, possibly via paracrine signaling.