bluuebunny/crossref_metadata_2025_split huggingface dataset:
SISTEM PENDUKUNG KEPUTUSAN PENENTUAN DOSEN BERPRESTASI MENGGUNAKAN METODE SIMPLE ADDATIVE WEIGHTING (STUDI KASUS FMIPA UHO)
Authors: Rial Muhammad , Saidi Laode , Surimi La , Hamundu Ferdinand Murni 
Published: December 2023

ABSTRAK
Pegawai adalah seseorang yang diberikan pekerjaan oleh orang yang memberikan pekerjaan kepadanya berdasarkan ketentuan yang sudah disepakati, untuk diangkat sebagai Pegawai ASN secara tetap oleh pejabat pembina kepegawaian untuk menduduki jabatan pemerintahan. Tujuan penelitian ini adalah untuk menghasilkan sistem pendukung keputusan menggunakan metode Simple Additive Weighting yang dapat membantu penyeleksi dalam menentukan pegawai berprestasi.
Penelitian ini menggunakan metode Simple Additive Weighting. Metode ini dapat menentukan nilai bobot untuk setiap kriteria, kemudian dilanjutkan dengan proses perankingan yang akan menyeleksi alternatif terbaik dari sejumlah alternatif sehingga penentuan dosen berprestasi dapat dilakukan secara objektif dengan nilai akhir yang tertinggi yang direkomendasikan untuk mendapatkan predikat pegawai berprestasi.
Dari hasil pengujian kinerja sistem menyatakan bahwa sistem mampu memberikan perankingan pegawai berprestasi dengan baik sesuai dengan kriteria yang digunakan. Fungsi penelitian ini adalah sistem yang dapat membantu user dalam mengolah data pegawai, menyeleksi pegawai, mempercepat proses penyeleksian serta mempermudah dalam menentukan pegawai berprestasi. Metode SAW yang diterapkan dalam sistem dapat digunakan untuk menentukan pegawai berprestasi dengan model perankingan yang sesuai dengan kriteria yang berlaku kemudian pengujian dengan menggunakan Black-box memberikan dokumentasi hasil pengujian yang menginformasikan kesesuaian sistem yang diuji dan dilanjutkan dengan perhitungan pengujian Confusion Matrix diperoleh tingkat akurasi sebesar 89%
Comparison of the Healthy Lifestyle Behaviors of Resident Russian Women and Their Children in Antalya with Their Turkish Peers: A Descriptive Study
Authors: EMİR Sevda , GÖZÜM Sebahat 
Published: December 2023

Objective: The migration movement from Russia to Antalya is predominantly women and children. The aim of this research was to compare the healthy lifestyle behaviors of Russian women residing in Antalya and their primary school children with their Turkish peers.
Methods: This is descriptive comparative research. The study with a purposive sample method included students in grades 1-8 of one private Russian primary school and their mothers, as well as students from three private Turkish schools in the same region and their mothers to ensure similarity. 122 Turkish and 76 Russian mothers and child couples participated in the study. Russian women and Turkish peers completed Healthy Lifestyle Behaviors Scale and the International Physical Activity Questionnaire. The Nutritional Behavior Scale and the Child Physical Activity Questionnaire (CPAQ) were fulfilled by children.
Results: Russian women were better healthy lifestyle behaviors than Turkish peers in health responsibility (z=-3,91; p
Trilliumosides K and L, two novel steroidal saponins from rhizomes of Trillium govanianum, as potent anti-cancer agents targeting apoptosis in the A-549 cancer cell line
Authors: Lone Bashir Ahmad , Tabassum Misbah , Bhushan Anil , Rani Dixhya , Dhiman Urvashi , Ahmad Ajaz , Mir Hilal Ahmad , Gupta Prem N. , Mondhe D. M. , Gairola Sumeet , Gupta Prasoon 
Published: December 2023

Two novel steroidal saponins, trilliumosides K (1) and L (2), were isolated from the rhizomes of Trillium govanianum led by bioactivity-guided phytochemical investigation along with seven known compounds: govanoside D (3), protodioscin (4), borassoside E (5), 20-hydroxyecdysone (6), 5,20-hydroxyecdysone (7), govanic acid (8), and diosgenin (9). The structure of novel compounds 1-2 was established using analysis of spectroscopic data including 1D and 2D nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HR-ESI-MS) data. All isolated compounds were evaluated for in vitro cytotoxic activity against a panel of human cancer cell lines. Compound 1 showed significant cytotoxic activity against the A-549 (Lung) and SW-620 (Colon) cancer cell lines with IC50 values of 1.83 and 1.85 µM, respectively whereas the IC50 value of Compound 2 against the A-549 cell line was found to be 1.79 µM. Among the previously known compounds 3, 5, and 9, the cytotoxic IC50 values were found to be in the range of 5–10 µM. Comprehensive anti-cancer investigation revealed that Compound 2 inhibited in vitro migration and colony-forming capability in the A-549 cell line. Additionally, the mechanistic analysis of Compound 2 on the A-549 cell line indicated distinctive alterations in nuclear morphology, increased reactive oxygen species (ROS) production, and decreased levels of mitochondrial membrane potential (MMP). By upregulating the pro-apoptotic protein BAX and downregulating the anti-apoptotic protein BCL-2, the aforementioned actions eventually cause apoptosis, a crucial hallmark in cancer research, which activates Caspase-3. To the best of our knowledge, this study reports the first mechanistic anti-cancer evaluation of the compounds isolated from the rhizomes of T. govanianum with remarkable cytotoxic activity in the desired micromolar range.
Indexes of ferroptosis and iron metabolism were associated with the severity of diabetic nephropathy in patients with type 2 diabetes mellitus: a cross-sectional study
Authors: Zhao Pingping , Lv Xiaoyu , Zhou Zhicong , Yang Xiaolan , Huang Ying , Liu Jingfang 
Published: December 2023

ObjectiveTo explore the correlations between diabetic nephropathy (DN) and serum levels of glutathione peroxidase 4 (GPX4), acyl-CoA synthetase long-chain family member 4 (ACSL4), iron, transferrin (Tf), and ferritin in patients with type 2 diabetes mellitus (T2DM).MethodsAccording to the urinary albumin excretion rate(UAER) or estimated glomerular filtration rate (eGFR) levels, a total of 123 patients with T2DM were separately divided into normoalbuminuria (NO), microalbuminuria (MI), macroalbuminuria (MA) groups, and G1 (eGFR ≥ 90 mL/min), G2 (eGFR ≤ 60 mL/min to &lt; 90 mL/min), and G3 groups (eGFR&lt; 60 mL/min), with 33 healthy participants as the control (HC). The differences in serum GPX4, ACSL4, iron, Tf, and ferritin levels between groups were compared, and the relationships between these levels were analysed. The independent correlations between UAER or DN severity and serum GPX4, ACSL4, iron, Tf, and ferritin levels were analysed by multiple linear and multinomial logistic regression, respectively.ResultsTo the patients with T2DM, with the increase in UAER levels, GPX4, iron, and Tf levels gradually decreased, whereas ACSL4 levels increased, meanwhile with the decrease in eGFR levels, GPX4 and Tf levels gradually decreased, whereas ACSL4 levels increased. UAER were independently and positively correlated with ACSL4 [β = 17.53, 95% confidence interval (CI; 11.94, 23.13)] and negatively correlated with GPX4 [β = −1.633, 95% CI (−2.77, -0.496)] and Tf [β = −52.94, 95% CI (-95.78, −10.11)].The NO and MI groups were considered as reference groups, respectively. The severity of DN was negatively correlated with serum GPX4 [odds ratio (OR) = 0.925 and 0.902, p =0.015 and 0.001], and Tf (OR = 0.109 and 0.119, p =0.043 and 0.034), and positively correlated with ACSL4 (OR = 1.952 and 1.865, both p &lt;0.001) in the MA group.ConclusionDN severity was negatively correlated with serum GPX4 and Tf levels and positively correlated with serum ACSL4 levels in patients with T2DM.
B-cell-specific MhcII regulates microbiota composition in a primarily IgA-independent manner
Authors: Roland Mary Melissa , Peacock Tori E. , Hall Nia , Mohammed Ahmed Dawood , Ball Ryan , Jolly Amy , Alexeev Sergei , Dopkins Nicolas , Nagarkatti Mitzi , Nagarkatti Prakash , Kubinak Jason L. 
Published: December 2023

BackgroundThe expression of major histocompatibility complex class II (MhcII) molecules on B cells is required for the development of germinal centers (GCs) in lymphoid follicles; the primary sites for the generation of T-cell-dependent (TD) antibody responses. Peyer’s patches (PPs) are secondary lymphoid tissues (SLOs) in the small intestine (SI) that give rise to high-affinity, TD antibodies (mainly immunoglobulin A (IgA)) generated against the microbiota. While several studies have demonstrated that MhcII antigen presentation by other immune cells coordinate TD IgA responses and regulate microbiota composition, whether or not B-cell-specific MhcII influences gut microbial ecology is unknown.MethodsHere, we developed a novel Rag1-/- adoptive co-transfer model to answer this question. In this model, Rag1-/- mice were reconstituted with naïve CD4+ T cells and either MhcII-sufficient or MhcII-deficient naïve B cells. Subsequent to this, resulting shifts in microbiota composition was characterized via 16S rRNA gene sequencing of SI-resident and fecal bacterial communities.ResultsResults from our experiments indicate that SLO development and reconstitution of an anti-commensal TD IgA response can be induced in Rag1-/- mice receiving T cells and MhcII-sufficient B cells, but not in mice receiving T cells and MhcII-deficient B cells. Results from our 16S experiments confirmed that adaptive immunity is a relevant host factor shaping microbial ecology in the gut, and that its impact was most pronounced on SI-resident bacterial communities.ConclusionOur data also clearly establishes that MhcII-mediated cognate interactions between B cells and T cells regulates this effect by maintaining species richness in the gut, which is a phenotype commonly associated with good health. Finally, contrary to expectations, our experimental results indicate that IgA was not responsible for driving any of the effects on the microbiota ascribed to the loss of B cell-specific MhcII. Collectively, results from our experiments support that MhcII-mediated antigen presentation by B cells regulates microbiota composition and promotes species richness through an IgA-independent mechanism.
HELIOS-expressing human CD8 T cells exhibit limited effector functions
Authors: Neyens Damien , Hirsch Thibault , Abdel Aziz Issa Abdel Hadi Achraqat , Dauguet Nicolas , Vanhaver Christophe , Bayard Alexandre , Wildmann Claude , Luyckx Mathieu , Squifflet Jean-Luc , D’Hondt Quentin , Duhamel Céline , Huaux Antoine , Montiel Virginie , Dechamps Mélanie , van der Bruggen Pierre 
Published: December 2023

IntroductionThe transcription factor HELIOS is primarily known for its expression in CD4 regulatory T cells, both in humans and mice. In mice, HELIOS is found in exhausted CD8 T cells. However, information on human HELIOS+ CD8 T cells is limited and conflicting. MethodsIn this study, we characterized by flow cytometry and transcriptomic analyses human HELIOS+ CD8 T cells.ResultsThese T cells primarily consist of memory cells and constitute approximately 21% of blood CD8 T cells. In comparison with memory HELIOS- T-BEThigh CD8 T cells that displayed robust effector functions, the memory HELIOS+ T-BEThigh CD8 T cells produce lower amounts of IFN-γ and TNF-α and have a lower cytotoxic potential. We wondered if these cells participate in the immune response against viral antigens, but did not find HELIOS+ cells among CD8 T cells recognizing CMV peptides presented by HLA-A2 and HLA-B7. However, we found HELIOS+ CD8 T cells that recognize a CMV peptide presented by MHC class Ib molecule HLA-E. Additionally, a portion of HELIOS+ CD8 T cells is characterized by the expression of CD161, often used as a surface marker for identifying TC17 cells. These CD8 T cells express TH17/TC17-related genes encoding RORgt, RORa, PLZF, and CCL20. DiscussionOur findings emphasize that HELIOS is expressed across various CD8 T cell populations, highlighting its significance beyond its role as a transcription factor for Treg or exhausted murine CD8 T cells. The significance of the connection between HELIOS and HLA-E restriction is yet to be understood.
Comparison of neoadjuvant chemoimmunotherapy and chemotherapy alone for resectable stage III non-small cell lung cancer: a real-world cohort study
Authors: Zhou Sihao , Liu Yi , Liu Kejun , Zhang Junkai , Liang Hanlin , Wu Yingmeng , Ye Hongyu , Liang Yi , Zhang Jingjing , Huang Weizhao 
Published: December 2023

BackgroundWe compared the real-world efficacy and safety of neoadjuvant chemoimmunotherapy to chemotherapy alone in patients with stage III non-small-cell lung cancer (NSCLC).Participants and methodsA total of 59 consecutive patients were finally selected and divided into two groups: the neoadjuvant chemotherapy group (n = 33) and the neoadjuvant chemoimmunotherapy group (n = 26). The primary endpoint was disease-free survival (DFS). The secondary endpoints were pathological response, clinical response, and adverse events. All patients were followed up to collect perioperative pathology and clinical data.ResultsThe objective response rate (ORR), pathological complete response (pCR), and major pathological response (MPR) were significantly higher in the neoadjuvant chemoimmunotherapy group than in the neoadjuvant chemotherapy group (73.1% vs. 45.5%, 34.6% vs. 3.0%, and 65.3% vs. 15.1%, respectively; P &lt; 0.05). There was no statistically significant difference in disease-free survival between the neoadjuvant chemoimmunotherapy and neoadjuvant chemotherapy groups (P = 0.129). Patients in the neoadjuvant chemoimmunotherapy group had a higher rate of tumor regression than those in neoadjuvant chemotherapy group (37.0% [25 patients] vs. 29.0% [33 patients], P = 0.018). However, no discernible correlation between MPR achievement and the degree of tumor shrinkage was observed in either group (P &gt; 0.05). The cumulative MPR rates were 42.3, 50, and 65.3% for 2, 3, and ≥ 4 cycles, respectively, in the neoadjuvant chemoimmunotherapy group and 9.1, 12.1, and 15.1% for ≤ 2, 3, and ≥ 4 cycles, respectively, in the neoadjuvant chemotherapy group. Moreover, No statistical difference was observed between the two groups regarding postoperative complications, resection range, operation time, surgical method, and extent of resection (P &gt; 0.05). Although the incidence of grades III–IV adverse events was higher in the neoadjuvant chemotherapy group than in the neoadjuvant chemoimmunotherapy group (33.3% vs. 4.6%, P = 0.042), there was no significant difference in the incidence of adverse events between the two groups (64.6% vs. 83.6%, P = 0.072).ConclusionIn stage III NSCLC, neoadjuvant chemoimmunotherapy achieved higher pathological and clinical remission rates than chemotherapy alone, with compromising safety, making it an attractive choice for neoadjuvant therapy.
Trawling-induced change in benthic effect trait composition – A multiple case study
Authors: Beauchard Olivier , Bradshaw Clare , Bolam Stefan , Tiano Justin , Garcia Clément , De Borger Emil , Laffargue Pascal , Blomqvist Mats , Tsikopoulou Irini , Papadopoulou Nadia K. , Smith Christopher J. , Claes Jolien , Soetaert Karline , Sciberras Marija 
Published: December 2023

IntroductionThe importance of the response-effect trait dichotomy in marine benthic ecology has garnered recent attention. Response traits, characterising species responses to environmental variations, have been a dominant focus in the development of ecological indicators for ecosystem health assessment. In contrast, effect traits, expressing effects of organism activities on the ecosystem, still do not benefit from an equal interest in spite of the complementary facet that they provide to complete our understanding of functional diversity and ecosystem vulnerability. In this study, we explore the consequences of disturbance by bottom trawl fisheries on benthic effect trait composition.MethodsTo this end, we used different contexts of environmental and trawling conditions from thirteen case studies in European waters and apply the same analytical procedure to derive a gradient that solely account for trawling-induced disturbance (Partial RLQ analysis).ResultsBottom trawling was found to be a selective force of benthic effect trait composition in a majority of case studies. In general, tube-dwelling species were more typical of low trawling frequencies, whereas deep burrowing species were more resistant at high trawling frequencies. Although we report significantly deleterious effects of trawling on benthic ecosystem functions, the effect trait pattern along the gradient was never related to life span, a key response trait generally assumed to express recoverability following disturbance. Furthermore, we show that trends in species multi-functionality and community functional diversity can be negative or positive along the trawling intensity gradient.DiscussionWe discuss the relevance of these results in light of recent developments in the framework of response and effect trait dichotomy, and provide guidelines of trait data analysis in the context of trawl fisheries impact on the sea floor. Our findings emphasize the importance of fundamental concepts from functional ecology in this context and represent a first step toward an assessment of trawling effect more oriented on benthos-mediated biogeochemical processes.
Analysis of the microbial diversity in takin (Budorcas taxicolor) feces
Authors: Ma Xiaoping , Wang Weichen , Cai Lijun , Xiao Mei , He Fang , Liu Zhen , Chen Dong , Wang Ya , Shen Limin , Gu Yu 
Published: December 2023

IntroductionThe intestinal tract of animals is a complex and dynamic microecosystem that is inextricably linked to the health of the host organism. Takin (Budorcas taxicolor) is a threatened species, and its gut microbiome is poorly understood. Therefore, this study aimed to analyze the microbial community structure and potential pathogens of takin.MethodsTakin fecal samples were collected from five sites in a nature reserve to ensure the uniformity of sample collection, determine the effects of different geographical locations on gut microbes, and analyze the differences in microbial communities between sites. Subsequently, high-throughput 16S rDNA gene sequencing was performed to analyze the microbial diversity and potential pathogens in the gut; the findings were verified by isolating and culturing bacteria and metagenomic sequencing.Results and discussionThe takin gut microflora consisted mainly of four phyla: Firmicutes (69.72%), Bacteroidota (13.55%), Proteobacteria (9.02%), and Verrucomicrobiota (3.77%), representing 96.07% of all microorganisms. The main genera were UCG-005 (20.25%), UCG-010_unclassified (12.35%), Firmicus_unclassified (4.03%), and Rumino coccsea_unclassified (3.49%), while the main species were assigned to Bacteria_unclassified. Potential pathogens were also detected, which could be used as a reference for the protection of takin. Pseudomonas presented the highest abundance at Shuichiping and may represent the main pathogen responsible for the death of takin at the site. This study provides an important reference for investigating the composition of the bacterial community in the intestine of takin.
Causal associations between gut microbiota and regional cortical structure: a Mendelian randomization study
Authors: Zhou Maochao , Chen Song , Chen Yan , Wang Chunhua , Chen Chunmei 
Published: December 2023

IntroductionObservational studies have reported associations between gut microbiota composition and central nervous system diseases. However, the potential causal relationships and underlying mechanisms remain unclear. Here, we applied Mendelian randomization (MR) to investigate the causal effects of gut microbiota on cortical surface area (SA) and thickness (TH) in the brain.MethodsWe used genome-wide association study summary statistics of gut microbiota abundance in 18,340 individuals from the MiBioGen Consortium to identify genetic instruments for 196 gut microbial taxa. We then analyzed data from 56,761 individuals from the ENIGMA Consortium to examine associations of genetically predicted gut microbiota with alterations in cortical SA and TH globally and across 34 functional brain regions. Inverse-variance weighted analysis was used as the primary MR method, with MR Egger regression, MR-PRESSO, Cochran’s Q test, and leave-one-out analysis to assess heterogeneity and pleiotropy.ResultsAt the functional region level, genetically predicted higher abundance of class Mollicutes was associated with greater SA of the medial orbitofrontal cortex (β = 8.39 mm2, 95% CI: 3.08–13.70 mm2, p = 0.002), as was higher abundance of phylum Tenericutes (β = 8.39 mm2, 95% CI: 3.08–13.70 mm2, p = 0.002). Additionally, higher abundance of phylum Tenericutes was associated with greater SA of the lateral orbitofrontal cortex (β = 10.51 mm2, 95% CI: 3.24–17.79 mm2, p = 0.0046). No evidence of heterogeneity or pleiotropy was detected.ConclusionSpecific gut microbiota may causally influence cortical structure in brain regions involved in neuropsychiatric disorders. The findings provide evidence for a gut-brain axis influencing cortical development, particularly in the orbitofrontal cortex during adolescence.
Efficacy and safety of rituximab in elderly patients with membranous nephropathy
Authors: Guo Yanhong , Zhao Huayan , Ren Mingjing , Wang Yulin , Wang Liuwei , Tang Lin 
Published: December 2023

Objectives: Advancing age is a risk factor for treatment-related side effects and mortality in membranous nephropathy (MN) patients treated with traditional immunosuppressive regimens. This study aimed to determine the efficacy and safety of rituximab (RTX) in the treatment of elderly patients with MN.Methods: We performed a single center retrospective review of 37 consecutive MN patients aged 70 and older at the time of RTX infusion. We also enrolled 76 young patients (&lt;70 years old) with MN as the control group. We assessed clinical and laboratory indices, remission rates, and adverse events at RTX infusion, 3 months, and last visit.Results: A total of 37 elderly patients with MN were included, with a median follow-up period of 15.50 (10.00, 24.40) months. Of the 37 patients, 75.68% were male, and mean age was 71.89 ± 2.47 years. At last visit, 7 (18.92%) patients achieved complete remission, and 26 (70.27%) patients achieved complete or partial remission. There were no differences in the complete remission rate and complete or partial remission rate at last visit compared to young patients (26.32% vs. 18.92%, p = 0.387; 85.53% vs. 70.27%, p = 0.055). After RTX treatment, three of 6 elderly patients with pneumonia died due to ineffective treatment of the infection in RTX therapy courses. The results of multivariant regression analysis showed that elderly patients have an increased risk of serious infection, compared with patients younger than 70 years (OR = 32.874, 95% CI 1.300–831.490, p = 0.034). For each increase of 1 g/L in serum albumin, the risk of serious infection would decrease by 43.2% (OR = 0.568, 95% CI 0.334–0.969, p = 0.038).Conclusion: This study demonstrates that RTX is effective in the treatment of elderly patients with MN. However, we also observed a high incidence of infectious complications. Our experience was limited by its retrospective design and relatively small sample size, and further randomized controlled studies with large sample size are needed to confirm our preliminary findings.