bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomal miR-223-3p from bone marrow mesenchymal stem cells targets HDAC2 to downregulate STAT3 phosphorylation to alleviate HBx-induced ferroptosis in podocytes
Authors: Chen Yueqi , Yang Xiaoqian , Feng Moxuan , Yu Yani , Hu Yongzheng , Jiang Wei 
Published: February 2024

Background: Hepatitis B virus associated-glomerulonephritis (HBV-GN) is one of the major secondary renal diseases in China, and microRNAs (miRNAs) in bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exo) can attenuate HBV-X protein (HBx)-induced ferroptosis in renal podocytes, but the exact mechanism remains unclear. This study aimed to investigate the protective mechanism of miR-223-3p in BMSC-Exo in HBx-induced ferroptosis in podocytes.Methods: The study employed human renal podocyte cells (HPCs), bone marrow-derived mesenchymal stem cells (BMSCs), as well as kidney tissue from C57BL/6 mice and HBx transgenic mice. Initially, the correlation between STAT3 phosphorylation and ferroptosis was authenticated through the administration of signal transducer and activator of transcription 3 (STAT3) phosphorylation inhibitors in both in vivo and in vitro settings. Furthermore, the effect of HDAC2 overexpression on STAT3 phosphorylation was examined. Subsequently, the association between BMSC-Exo carrying miR-223-3p, HDAC2, and the phosphorylation of STAT3 in HPCs ferroptosis and injury induced by HBx was assessed. The interaction between miR-223-3p and HDAC2 was confirmed via RNA immunoprecipitation assay. Various techniques such as cell counting kit-8 assay, western blot, RT-qPCR, immunofluorescence, flow cytometry, lipid peroxidation assay kit, iron assay kit, transmission electron microscopy, and hematoxylin-eosin staining were employed to visualize the extent of HBx-induced podocyte injury and ferroptosis in both in vivo and in vitro.Results: The attenuation of podocyte ferroptosis can be achieved by inhibiting the phosphorylation of STAT3 in podocytes induced by HBx. Conversely, the upregulation of HDAC2 can enhance STAT3 phosphorylation, thereby promoting podocyte ferroptosis. MiR-223-3p was capable of directly exerting negative regulation on HDAC2 expression. BMSC-Exo carrying miR-223-3p can effectively suppress the expression of HDAC2, ultimately leading to reduce HBx-induced ferroptosis in podocytes by targeting HDAC2 with miR-223-3p and downregulating STAT3 phosphorylation.Conclusion: This study evidences the potential of BMSC-Exo mediated delivery of miR-223-3p in mitigating HBx-induced ferroptosis in podocytes, thereby offering a novel therapeutic target and approach for treating HBV-GN and alleviating renal injury.
Case report: Sustained complete remission with all-oral MEPED therapy in a patient with Hodgkin’s disease developing resistance to pembrolizumab
Authors: Reuthner K. , Aubele P. , Menhart K. , Rath P. , Harrer D. C. , Herr W. , Hahn J. , Vogelhuber M. , Heudobler D. , Lueke F. , Reichle A. , Grube M. 
Published: February 2024

Targeted chemotherapy and immune checkpoint inhibitors (ICPi) have expanded the spectrum of therapies for patients with relapsed/refractory (r/r) Hodgkin’s disease and significantly improved the proportion of patients with long-term disease control. However, there is no standardized therapeutic option in case of further progression. Recently, we demonstrated that therapy with MEPED (metronomic chemotherapy, everolimus, pioglitazone, etoricoxib, dexamethasone) is highly effective in patients with r/r Hodgkin’s disease. The benefit after pre-treatment with ICPi has not been studied, yet. Here, we report a patient with progressive Hodgkin’s disease on Pembrolizumab for the first time who achieved sustained complete remission (CR) after initiation of MEPED therapy. A 57-year-old patient was pre-treated with brentuximab vedotin for relapsed advanced Hodgkin’s disease and had received Pembrolizumab for progression from November 2020 to July 2022. Due to further progression, MEPED therapy was started in August 2022 and continued until May 2023. It consisted of a strictly oral daily (28-day cycle) application of low-dose treosulfan 250 mg, everolimus 15 mg, pioglitazone 45 mg, etoricoxib 60 mg, and dexamethasone 0.5 mg. Treatment response was evaluated by F-18 FDG-PET/CT (PET/CT). CR was defined by a negative Deauville score (DS) of 1-3. Already 3 months after starting MEPED, a CR (DS: 3) was confirmed by PET/CT in November 2022. The next follow-up in May 2023 continued to show CR (DS: 3). The therapy was very well tolerated. No hematological or other organ toxicity was observed. However, in May 2023 the patient presented with leg edema and weight gain, most likely due to pioglitazone and the PET/CT revealed suspected everolimus-induced pneumonitis, so MEPED was discontinued and diuretic therapy and treatment with prednisolone was started with gradual dose reduction. This resulted in a rapid complete resolution of the symptoms. The next PET-CT in July 2023 continued to show CR (DS: 3) without evidence of pneumonitis. Currently, therapy with MEPED has not been resumed. In conclusion, we demonstrate for the first time that MEPED therapy is highly effective in a patient with Hodgkin’s disease who has been refractory to ICPi. Sustained CR was achieved over 11 months after initiation of MEPED therapy. Further studies on a larger patient cohort should be performed.
Changes of brain-derived neurotrophic factor (BDNF) levels after different exercise protocols: a systematic review of clinical studies in Parkinson’s disease
Authors: Paterno Andrea , Polsinelli Giovanni , Federico Bruno 
Published: February 2024

Background: Brain-Derived Neurotrophic Factor (BDNF) serum levels are reduced in patients with Parkinson’s Disease (PD).Objectives: This study aimed to assess the effect of exercise intensity, volume and type on BDNF levels in patients with PD.Methods: We searched clinicaltrials.gov, CINAHL, Embase, PubMed, Scopus, Web of Science for both controlled and non-controlled studies in patients with PD, published between 2003 and 2022, which assessed Brain-Derived Neurotrophic Factor before and after different exercise protocols. Exercise intensity was estimated using a time-weighted average of Metabolic Equivalent of Task (MET), while exercise volume was estimated by multiplying MET for the duration of exercise. Exercise types were classified as aerobic, resistance, balance and others. We computed two distinct standardized measures of effects: Hedges’ g to estimate differences between experimental and control group in pre-post intervention BDNF changes, and Cohen’s d to measure pre-post intervention changes in BDNF values for each study arm. Meta-regression and linear regression were used to assess whether these effect measures were associated with intensity, volume and type. PROSPERO registration number: CRD42023418629.Results: Sixteen studies (8 two-arm trials and 8 single-arm trials) including 370 patients with PD were eligible for the systematic review. Selected studies had a large variability in terms of population and intervention characteristics. The meta-analysis showed a significant improvement in BDNF levels in the exercise group compared to the control group, Hedges’ g = 0.70 (95% CI: 0.03, 1.38), with substantial heterogeneity (I2 = 76.0%). Between-group differences in intensity were positively associated with change in BDNF in a subset of 5 controlled studies. In the analysis which included non-controlled studies, intensity and total exercise volume were both positively associated with BDNF change. No difference was found according to exercise type.Conclusion: Exercises of greater intensity may increase BDNF levels in patients with PD, while the role of volume of exercise needs to be further explored.
Feasibility and acceptability of the smart pillbox and medication label with differentiated care to support person-centered tuberculosis care among ASCENT trial participants – A multicountry study
Authors: Tadesse Amare W. , Mganga Andrew , Dube Tanyaradzwa N. , Alacapa Jason , van Kalmthout Kristian , Letta Taye , Mleoh Liberate , Garfin Anna M. C. , Maraba Noriah , Charalambous Salome , Foster Nicola , Jerene Degu , Fielding Katherine L. 
Published: February 2024

IntroductionDigital adherence technologies (DATs) can offer alternative approaches to support tuberculosis treatment medication adherence. Evidence on their feasibility and acceptability in high TB burden settings is limited. We conducted a cross-sectional survey among adults with drug-sensitive tuberculosis (DS-TB), participating in pragmatic cluster-randomized trials for the Adherence Support Coalition to End TB project in Ethiopia (PACTR202008776694999), the Philippines, South Africa and Tanzania (ISRCTN 17706019).MethodsFrom each country we selected 10 health facilities implementing the DAT intervention (smart pillbox or medication labels, with differentiated care support), ensuring inclusion of urban/rural and public/private facilities. Adults on DS-TB regimen using a DAT were randomly selected from each facility. Feasibility of the DATs was assessed using a standardized tool. Acceptability was measured using a 5-point Likert-scale, using the Capability, Opportunity, Motivation, Behavior (COM-B) model. Mean scores of Likert-scale responses within each COM-B category were estimated, adjusted for facility-level clustering. Data were summarized by country and DAT type.ResultsParticipants using either the pillbox (n = 210) or labels (n = 169) were surveyed. Among pillbox users, phone ownership (79%), use of pillbox reminders (87%) and taking treatment without the pillbox (22%) varied by country. Among label users, phone ownership (81%), paying extra to use the labels (8%) and taking treatment without using labels (41%) varied by country. Poor network, problems with phone charging and access, not having the pillbox and forgetting to send text were reasons for not using DATs. Overall, people with TB had a favorable impression of both DATs, with mean composite scores between 4·21 to 4·42 across COM-B categories. Some disclosure concerns were reported.ConclusionFrom client-perspective, pillboxes and medication labels with differentiated care support were feasible to implement and acceptable in variety of settings. However, implementation challenges related to network, phone access, stigma, additional costs to people with TB to use DATs need to be addressed.
Ecological and Syntaxonomic Analysis of the Communities of Glebionis coronaria and G. discolor (Malvion neglectae) in the European Mediterranean Area
Authors: Cano Eusebio , Cano-Ortiz Ana , Quinto Canas Ricardo , Piñar Fuentes Jose Carlos , Rodrigues Meireles Catarina , Raposo Mauro , Pinto Gomes Carlos , Laface Valentina Lucia Astrid , Spampinato Giovanni , Musarella Carmelo Maria 
Published: February 2024

Nitrophilous communities dominated by Glebionis coronaria and Glebionis discolor in the European Mediterranean area were studied. The nomenclature was corrected according to the current taxonomy, following the International Code of Phytosociological Nomenclature (ICPN). The statistical analysis revealed six new associations and one subassociation, with four in Spain, one in Greece, and one in Italy. Additionally, a subassociation of high relevance due to its endemic character was identified. These grasslands exhibit requirements for organic matter and other edaphic nutrients that are closer to those of Malva neglecta communities than to those of Hordeum murinum subsp. leporinum. We confirmed the published syntaxon with the rank of Resedo albae-Glebionenion coronariae suballiance and its subordination to the Malvion neglectae alliance, and we established the type association for this suballiance. Sisimbrietalia officinalis J. Tüxen in Lohmeyer et al. 1962 em. Rivas-Martínez, Báscones, T. E. Díaz, Fernández-González & Loidi 1991. Stellarietea mediae Tüxen, Lohmeyer & Preising ex von Rochow 1951.
ATLAS: Latest Advancements and First Observations
Authors: Pandeirada João , Bergano Miguel , Marques Paulo , Coelho Bruno , Barbosa Domingos , Figueiredo Mário 
Published: February 2024

The increasing amount of space debris poses a significant threat to operational satellites and space-based services. This article updates the community on the current status of the development of ATLAS, a tracking radar that is part of the EUSST network and aims to detect space objects in low Earth orbits. This article focuses on the latest activities performed: calibration of the pointing system and initial observations of space objects. The calibration procedure consisted of cross-scanning the Solar disk and yielded great results, obtaining an offset of 5.3° in azimuth and 0.10° in elevation. The first observation campaign resulted in 33 range detections of the International Space Station (ISS) with a probability of false alarm of 10−9. The observations were then used to readjust the radar equation to assess the real-world performance of the system.
BIOCONVERSION OF PROTEIN AND FEED ENERGY INTO MEAT PRODUCTS OF HEIFERS OF DIFFERENT GENOTYPES
Authors: Косилов В.И. , Миронова И.В. , Долженкова Г.М. , Хабибуллин Р.М. , Галиева З.А. 
Published: February 2024

В статье приводятся результаты оценки эффективности трансформации питательных веществ и энергии кормов рациона чистопородными тёлками чёрно-пёстрой породы (I группа), её помесями с голштинами первого поколения (½ голтштин × ½ чёрно-пёстрая – II группа) и помесями второго поколения (¾ голштин × ¼ чёрно-пёстрая – III группа). Новизна работы заключается в том, что исследования такого плана при использовании в качестве объекта чистопородных и помесных тёлок проведены впервые. Установлено, что помесные тёлки II и III групп отличались меньшим, чем чистопородные сверстницы I группы, расходом сырого протеина и энергии на 1 кг прироста живой массы на 2,35–3,78% и 4,58–10,62% соответственно. При этом чистопородные тёлки I группы уступали помесям II и III групп по массе съедобных частей туши на 11,20–16,68 кг (6,85–10,21%), содержанию белка – на 2,82–4,61 кг (9,58–15,65%), экстрагируемого жира – на 3,68–5,26 кг (17,79–24,24%). Помесный молодняк II и III групп превосходил чистопородных сверстников I группы по выходу белка, экстрагируемого жира и энергии на 1 кг предубойной живой массы. Коэффициент биоконверсии протеина корма в мясной продукции у тёлок I, II и III групп в белок находился на уровне 7,25%, 7,53%, 7,97%, а энергии – 6,15%, 6,40% и 6,51% соответственно.
The results of the efficiency assessment of nutrients transformation and energy of ration feed by purebred Black-and-White heifers (group I), its crossbreeds with the first Holstein generation (½ Holtstein × ½ Black-and-White – group II) and second generation crossbreeds (¾ Holstein × ¼ Black-and-White – group III) are given in article. The novelty of the work is that research of this kind using purebred and mixed bred heifers as an object was carried out for the first time. It was established that mixed bred heifers of groups II and III differed in less than the purebred herdmates of group I the consumption of raw protein and energy per 1 kg of live weight gain by 2.35–3.78% and 4.58–10.62%, respectively. At the same time, purebred heifers of group I were inferior to crossbreds of groups II and III in terms of weight of edible parts of the carcass by 11.20–16.68 kg (6.85–10.21%), protein content – by 2.82–4.61 kg (9.58–15.65%), extractable fat – by 3.68–5.26 kg (17.79–24.24%). Mixed bred young animals of groups II and III were superior to purebred herdmates of group I in terms of protein yield, extractable fat and energy per 1 kg of preslaughter live weight. The bioconversion coefficient of the feed protein in meat products in heifers of groups I, II and III into protein was at the level of 7.25%, 7.53%, 7.97%, and energy – 6.15%, 6.40% and 6. 51%, respectively.
Multi-focus manipulation system based on separable natural evolution strategy aberration self-calibration
Authors: Liu Linxian , Liu Jiahao , Ding Chunxu , Yang Jiamiao , Gao Jia , Qu Yuan , He Qiaozhi , Shao Rongjun 
Published: February 2024

Abstract
Wavefront shaping using digital micromirror devices (DMDs) allows inertia-free focus manipulation with numerous modulation modes and high refresh rates. However, the aberration caused by the curvature of DMDs affects the focusing performance. Here, we propose an aberration self-calibration method based on separable natural evolution strategies. This method searches optimal Zernike coefficients of aberration globally and completes compensation using super-pixel encoding. Compared to the genetic algorithm method, we improve the speed by 62% and achieve better-focused spots. The method enables simultaneous scanning of 25 independent focal spots. This advancement supports wavefront-shaping applications in optical imaging, industrial inspection, and laser processing.
A two-stage insulation method for suppressing thermal crosstalk in microarray sensitive units
Authors: Zhu Shining , Li Xin , Luo Zhengjie , Jia Xuguang , Qin Yue , Guo Hao , Tang Jun , Li Zhonghao , Wen Huanfei , Ma Zongmin , Liu Jun 
Published: February 2024

Abstract
Thermal crosstalk between array structures is a key factor in limiting the sensitivity of micro-nano array sensors. We propose a two-stage thermally isolated structure with thermal holes and heat dissipation layer and pulsed voltage heating to reduce thermal crosstalk. Through finite element thermal simulation analysis as well as thermal interference test, the results show that the thermal crosstalk of the two-stage structure is reduced by 12.89% and 39.67%, respectively, in the steady state compared to the structure with no thermal isolation, and pulsed voltage heating leads to the thermal crosstalk of the two-stage structure to be <10%.
Extraction of interfacial thermal resistance across an organic/semiconductor interface using optical-interference contactless thermometry
Authors: Yu Jiawen , Hanafusa Hiroaki , Higashi Seiichiro 
Published: February 2024

Abstract
We have developed an experimental method to extract interfacial thermal resistance (ITR) at an organic/semiconductor interface based on optical-interference contactless thermometry. The proposed technique was applied to a SU-8/SiC bilayer sample, and clear oscillations in reflectivity induced by optical interference during pulse heating and cooling were observed. After fitting the observed reflectivity waveform with simulation results by a two-dimensional (2D) double-layer heat conduction model and multi-reflection calculations, ITR was extracted as 190 mm2 K W−1, which resulted in a temperature drop of 11 K at the interface. Moreover, the 2D transient temperature distribution of the sample throughout pulse heating and cooling was obtained.
UPAYA PENGENDALIAN RESISTENSI ANTIBIOTIK MELALUI PENYERAHAN ANTIBIOTIK SECARA TEPAT DI APOTEK WILAYAH SEBERANG ULU PALEMBANG
Authors: Simamora Sarmalina , Subiyandono Subiyandono , Sarmadi Sarmadi , Tedi Tedi 
Published: February 2024

Penjualan antibiotik tanpa resep dokter, khususnya sediaan oral maupun parenteral sesungguhnya adalah hal yang tidak benar, karena antibiotik termasuk golongan obat keras. Beberapa jenis obat keras memang dapat diserahkan oleh Apoteker dalam jumlah tertentu, termasuk antibiotika jenis sediaan topikal. Penjualan antibiotika oral secara bebas dapat menjadi pemicu terjadinya resiko yang tidak diinginkan. Selain resiko efek samping, resistensi bakteri merupakan resiko yang berdampak luas pada masyarakat.
Pengetahuan merupakan salah satu faktor yang mempengaruhi prilaku petugas kesehatan, demikian juga petugas apotek. Pengetahuan yang rendah menimbulkan rendahnya kepedulian akan akibat dari penggunaan antibiotika secara sembarangan.Pengetahuan dan perilaku ini dapat ditingkatkan melalui berbagai upaya, salah satunya adalah dengan pendekatan edukasi yang bersifat informal. Cara ini dapat dilakukan dalam berbagai jenis situasi dan berbagai tingkatan pengetahuan. Karena sifatnya yang informal kegiatan ini dilakukan di lokasi apotek wilayah Seberang Ulu Palembang ( 17 apotek).
Pengetahuan petugas yang umumnya masih rendah sampai sedang, terutama pemahaman akan Obat Wajib Apotek menjadi alasan mereka menyerahkan antibiotik tanpa resep dokter. Setelah diberikan edukasi, terdapat perubahan prilaku petugas sekalipun belum seluruhnya. Beberapa apotek sudah tidak melayani penjualan antibiotik tanpa resep, sedang yang lainnya sudah memberikan KIE (Komunikasi, Informasi dan Edukasi) saat penyerahan obat.
Effectiveness, Safety, and Costs of Thromboprophylaxis with Enoxaparin or Unfractionated Heparin Among Medical Inpatients With Chronic Obstructive Pulmonary Disease or Heart Failure
Authors: Amin Alpesh N. , Kartashov Alex , Ngai Wilson , Steele Kevin , Rosenthal Ning 
Published: February 2024

Background: Chronic obstructive pulmonary disease (COPD) and heart failure (HF) are risk factors for venous thromboembolism (VTE). Enoxaparin and unfractionated heparin (UFH) help prevent hospital-associated VTE, but few studies have compared them in COPD or HF. Objectives: To compare effectiveness, safety, and costs of enoxaparin vs UFH thromboprophylaxis in medical inpatients with COPD or HF. Methods: This retrospective cohort study included adults with COPD or HF from the Premier PINC AI Healthcare Database. Included patients received prophylactic-dose enoxaparin or UFH during a >6-day index hospitalization (the first visit/admission that met selection criteria during the study period) between January 1, 2010, and September 30, 2016. Multivariable regression models assessed independent associations between exposures and outcomes. Hospital costs were adjusted to 2017 US dollars. Patients were followed 90 days postdischarge (readmission period). Results: In the COPD cohort, 114 174 (69%) patients received enoxaparin and 51 011 (31%) received UFH. Among patients with COPD, enoxaparin recipients had 21%, 37%, and 10% lower odds of VTE, major bleeding, and in-hospital mortality during index admission, and 17% and 50% lower odds of major bleeding and heparin-induced thrombocytopenia (HIT) during the readmission period, compared with UFH recipients (all P <.006). In the HF cohort, 58 488 (58%) patients received enoxaparin and 42 726 (42%) received UFH. Enoxaparin recipients had 24% and 10% lower odds of major bleeding and in-hospital mortality during index admission, and 13%, 11%, and 51% lower odds of VTE, major bleeding, and HIT during readmission (all P <.04) compared with UFH recipients. Enoxaparin recipients also had significantly lower total hospital costs during index admission (mean reduction per patient: COPD, $1280; HF, $2677) and readmission (COPD, $379; HF, $1024). Among inpatients with COPD or HF, thromboprophylaxis with enoxaparin vs UFH was associated with significantly lower odds of bleeding, mortality, and HIT, and with lower hospital costs. Conclusions: This study suggests that thromboprophylaxis with enoxaparin is associated with better outcomes and lower costs among medical inpatients with COPD or HF based on real-world evidence. Our findings underscore the importance of assessing clinical outcomes and side effects when evaluating cost-effectiveness.