bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sexual Support Experiences of Australian Adults Living with a Spinal Cord Injury
Authors: Doig Connor , Allen Andrew , Mason Jonathan , Heck Marita , Cudmore Timothy 
Published: November 2023

Abstract
Objectives: Sexuality is a key domain of wellbeing that is significantly compromised by SCI but often left unaddressed during rehabilitation, with a typical focus being genital functioning and fertility. Using a theory-driven qualitative methodology and applying Material, Discursive, Intrapsychic Theory (encompassing multiple dimensions of sexuality), this study explored the support experiences of nine Australian adults with an SCI.
Design: This qualitative methodology took a deductive, critical and constructionist theoretical perspective, which prioritised the collection of information-rich data over acquiring a large sample size, and utilised a contextual approach to understanding the facets of sexual wellbeing.
Setting & Methods: Male (N = 8) and female (N = 1) participants with varying levels of SCI completed an online interview which was primarily participant-led.
Results: Participants’ responses reflected previous research on sexuality in SCI, noting an emphasis on physical aspects (e.g., erection and ejaculation ability) to the neglect of the psychological and interpersonal. The three core components of Material, Discursive, Intrapsychic Theory were mapped onto themes from the data, with multiple sub-themes comprised in each, demonstrating that aspects aside from sensation loss like gender identity, self-confidence, and disability stereotypes all effect sexual wellbeing. An additional fourth theme of relationship dynamics was also identified, including aspects such as connectedness and perceived safety in relationships.
Conclusions: This provides practical implications for support experiences such as the inclusion of partner support in rehabilitation. Further investigation is recommended to provide specific interventions for regaining sexuality in the context of a SCI.
Giant electric field-induced second harmonic generation in polar skyrmions
Authors: Li Qian , Wang Sixu , Li Wei , Deng Chenguang , Hong Zijian , Gao Hanbin , Li Xiaolong , Gu Yueliang , Zheng Qiang , Wu Yongjun , Evans Paul , Li Jing-Feng , Nan Ce-Wen 
Published: November 2023

Abstract
Electric field-induced second harmonic generation (EFISH) allows electrically controlling nonlinear light-matter interactions crucial for emerging integrated photonics applications. Despite its wide presence in materials, the figures-of-merit of EFISH are yet to be elevated to enable novel device functionalities. Here, we show that the polar skyrmions, a topological phase spontaneously formed in PbTiO3/SrTiO3 ferroelectric superlattices, exhibit an outstanding comprehensive EFISH performance. The second-order nonlinear susceptibility and modulation depth, measured under non-resonant 800 nm excitation, reach ~54.2 pm V-1 and ~664% V-1, respectively, and high response bandwidth (at gigahertz scales), wide operating temperature range (up to ~400 K) and good fatigue resistance (>1010 cycles) are also demonstrated. Through combined in-situ experiments and phase-field simulations, we establish the microscopic links between the exotic polarization configuration and field-induced transition paths of the skyrmions and their EFISH response. Our study not only presents a highly competitive thin-film material platform ready for constructing on-chip devices, but opens up new avenues of utilizing topological polar structures in the fields of photonics and optoelectronics.
Cenozoic India-Asia collision driven by mantle dragging the cratonic root
Authors: Li Yanchong , Liu Lijun , Peng Diandian , Cao Zebin 
Published: November 2023

Abstract

The driving force behind the Cenozoic Indian-Asia collision remains elusive. Using global-scale geodynamic modeling, we find that the continuous motion of the Indian plate is driven by a prominent upper-mantle flow pushing the thick Indian lithospheric root, originated from the northward rollover of the detached Neo-Tethyan slab and sinking slabs below East Asia. The maximum mantle drag occurs within the strong Indian lithosphere and is comparable in magnitude to that of slab pull (1013 N/m). The thick cratonic root enhances both lithosphere-asthenosphere coupling and upper-plate compressional stress, thereby sustaining the topography of Tibetan Plateau. We show that the calculated resistant force from the India-Asia plate boundary is also close to that due to the gravitational potential energy of Tibetan Plateau. Our findings suggest that this mantle flow is key for the formation of the Tibetan Plateau and represents part of a hemispheric convergent flow pattern centered on central Asia.
Deciphering the influence of centrosome-related genes on osteosarcoma to develop a prognostic signature
Authors: Zhong Yujian , Wei Sixing , Zeng Xiaoshuang , Jin Wenyi , Chen Junwen , Zhong Changheng , Huang Liangkun , Zhu Zhengjie , Xu Hanzhe , Zhou Jianlin , Peng Hao 
Published: November 2023

Abstract
The centrosome, a vital component in mitosis in eukaryotes, plays a pivotal role in cancer progression by influencing the proliferation and differentiation of malignant cells, making it a significant therapeutic target. We collected genes associated with centrosomes from existing literature and established a prognostic model for 85 osteosarcoma patients from the TARGET database. Genes associated with prognosis were identified through univariate Cox regression. We then mitigated overfitting by addressing collinearity using LASSO regression. Ultimately, a set of five genes was selected for the model through multivariable Cox regression. Model performance was assessed using ROC curves, which yielded a training set AUC of 0.965 and a validation set AUC of 0.770, indicating satisfactory model performance. We further identified genes with differential expression in high and low-risk groups and conducted functional enrichment analysis using KEGG, GO, Progeny, GSVA, and GSEA. Results revealed significant variances in various immune-related pathways between high and low-risk cohorts. Analysis of the immune microenvironment using ssGSEA and ESTIMATE indicated that individuals with unfavorable prognoses had lower immune scores, stromal scores, and ESTIMATE scores, coupled with higher tumor purity. This suggests that high-risk individuals have compromised immune microenvironments, potentially contributing to their unfavorable prognoses. Additionally, drug sensitivity and molecular docking analysis revealed increased responsiveness to paclitaxel in high-risk individuals, implying its prognostic value. The JTB-encoded protein exhibited a negative binding energy of -5.5 kcal/mol when interacting with paclitaxel, indicating its potential to enhance the patient's immune microenvironment. This framework enables patient prognosis prediction and sheds light on paclitaxel's mechanism in osteosarcoma treatment, facilitating personalized treatment approaches.
Predictive value of spectral dual-detector computed tomography for PD-L1 expression in stage I lung adenocarcinoma: Development and validation of a nomogram
Authors: Wang Tong , Fan Zheng , Yue Yong , Lu Xiaomei , Deng Xiaoxu , Hou Yang 
Published: November 2023

Abstract

Background
Programmed death ligand 1 (PD-L1) expression is a predictive biomarker for the efficacy of immune checkpoint inhibitors in treating early-stage lung adenocarcinoma (LA). Given that studies on the relationship between PD-L1 expression and spectral computed tomography (SDCT) quantification, qualitative parameters, and clinical biomarkers are limited, we aimed to explore this aspect for stage I LA and developed a nomogram to aid in preoperative individualized identification.
Methods
We analyzed SDCT parameters and PD-L1 expression in patients with invasive non-mucinous LA diagnosed by postoperative pathology. Based on the expression of PD-L1 (threshold, 1%), the patients were divided into positive and negative groups.
Results
Overall, 356 participants were included in the analysis. After univariate analysis, 18 parameters were associated with PD-L1 expression. Multiple regression analysis was performed on the significant parameters with AUC > 0.6, further, CT.value, CT40kev.a, Zeff.a, ED.a, ED.v, TK1, and Dsolid were found to be independent risk factors for PD-L1 expression in stage I LA. The clinical decision-making curve of the SDCT parameter-clinical nomogram had the highest net benefit over a wide range of threshold probabilities, and the clinical impact curve indicated its clinical validity. Model comparisons revealed the higher discriminatory accuracy of the nomogram than that of any individual variable.
Conclusion
Quantitative parameters based on SDCT can predict the expression of PD-L1 in early-stage LA, with Zeff.a playing a prominent role; our nomogram showed excellent predictive performance and good calibration. This approach may promote better non-invasive prediction of PD-L1 expression.
Changes in Abortion Accessibility and Its Effects: Assessing the Impact of the Dobbs Decision
Authors: Burkey Mark L. , Li Huan , Kelly Stephanie 
Published: November 2023

Abstract
Background: Previous research studies have used natural experiments to estimate the causal effects of changes in geographic accessibility to abortion clinics in the U.S. These effects include reductions in observed abortions, and births of so-called “marginal children” due to decreased access to abortion who may be raised in poor conditions. We measure changes in geographic accessibility to abortion procedures after the Dobbs decision repealed nationwide accessibility to abortion in the U.S., describe neighborhoods that are most affected, and use the results of previous studies to make estimates of the effects.
Methods: This paper measures road network distances and estimated travel times from each Census block group to the nearest abortion procedure provider before, and after the Dobbs decision when 21 states prohibited most abortion procedures ("Ban States"). These block groups comprise 236,883 neighborhoods of around 1,400 people each. Using the state of legislation changes at the end of 2022, we describe the socioeconomic characteristics of the neighborhoods most affected by the decision, and to forecast the changes in abortion rates and births in 2023.
Results: We find that the median distance to the nearest abortion provider nearly doubled to 24.68 miles, while the average distance for those in the 4th quartile increased from 82 to 364 miles. We estimate that more than 30,000 additional children will be born in 2023 due to the Dobbs decision. The neighborhoods that are the most affected by the Dobbs decision have high rates of families lacking in health insurance, education and internet connectivity, and high concentrations of people of color.
Conclusion: This study provides estimates of the changes in geographical accessibility to abortion clinics as a result of the Dobbs decision. The findings suggest that the impact extended beyond women in the 21 Ban States, affecting approximately 900,000 women in other states who will also experience reduced access to abortion services. The magnitude of the impacts and the demographic profile of those impacted will vary over time as states change their laws, but observing a snapshot of some of the impacts in 2023 is an important part of understanding this change in abortion policies.
Staphylococcus aureus pneumonia complicated with lower limb gangrene secondary to influenza A virus：a case report
Authors: Zhu Xiao-Ling , Zhang Juan , Zhang Dong-Ping , Wang Mao-Juan , Zhang Lian , Xie Hong 
Published: November 2023

Abstract

Background Millions of people worldwide die from lower respiratory tract infections every year, and a significant portion of these deaths can be attributed to seasonal influenza virus infections, due to the continuous emergence of new variants of influenza A virus (IAV) and secondary bacterialinfection with other viruses or bacteria. This report describes a female patient with IAV who secondary bacterial infection with Staphylococcus aureus and lower limb gangrene.
Case presentation The patient was a 39-year-old female who had persistent cough, sputum and tight breath for 2 days, worsening for 11+hours. She received VV-ECMO treatment for severe pneumonia. Her alveolar lavage fluid showed Staphylococcus aureus, Influenza A virus was positive for H3N2. After anti infection treatment, the patient's condition improved, and gangrene occurred in the lower limbs and amputation surgery was performed.
Conclusions Subsequent aureus infections after influenza can exacerbate respiratory failure in patients, leading to multiple organ dysfunction and even endangering patient safety. Early identification, identification of pathogens, and effective anti-infection treatment can improve prognosis.
The phthalocyanine derivative is less cytotoxic and does not impair in vitro wound healing compared to chlorhexidine
Authors: Santos Caique Andrade , Pessoa Adriano de Souza , Vilhena Fabiano Vieira , de Oliveira Rodrigo Cardoso , Santos Paulo Sérgio da Silva , Damante Carla Andreotti , Zangrando Mariana Schutzer Ragghianti 
Published: November 2023

Abstract
Objectives: To compare the cytotoxicity of different concentrations of a phthalocyanine (PHY) to chlorhexidine (CHX) and the influence of these compounds on fibroblast cell migration.
Methodology: Different concentrations of CHX and PHY (0.0075% to 0.12%) were evaluated using NIH 3T3 fibroblasts. Cell viability was assessed by the MTT and crystal violet (CV) assay; CHX and PHY (0.0075% and 0.12%) were also evaluated by in vitro wound healing assay.
Results: PHY was less cytotoxic compared to CHX, based on cell viability assays. PHY did not interfere with experimental healing, allowing cell migration similar to the positive control with both concentrations (PHY 0.0075% and 0.12%) and only 0.0075% CHX allowed cell migration. In a comparative analysis, PHY showed less cytotoxicity than CHX and PHY concentrations of 0.0075% and 0.015% was non-toxic even after 48 hours of contact with the cells.
Conclusion: This in vitroevaluation demonstrated that PHY was less cytotoxic to NIH 3T3 fibroblasts compared to CHX. Furthermore, the different concentrations of PHY did not interfere negatively in the healing of experimental wounds.
Clinical Relevance: Due to the observed clinical adverse effects and cytotoxicity, it becomes necessary to search for alternatives to CHX. Respecting the limitations of the current in vitro study, PHY showed satisfactory and promising results as a potential alternative to CHX.
Resected lung adenocarcinoma with lymph node metastasis: Is ground glass opacity component a prognostic factor?
Authors: Deng Chaoqiang , Jiang Chenyu , Ma Xiangyi , Fu Fangqiu , Wang Shengping , Li Yuan , Zhang Yang , Chen Haiquan 
Published: November 2023

Abstract
Purpose
Ground glass opacity (GGO)-featured lung adenocarcinoma generally has excellent prognosis, and rarely has lymph node metastasis. We aim to explore the prognostic impact of GGO component in node-positive lung adenocarcinomas.
Methods
A total of 669 patients with pathologic N1/N2 lung adenocarcinoma receiving R0 resection and systemic lymph node dissection from 2008 to 2015 were reviewed, including 635 solid and 34 part-solid lesions. Propensity score matching (PSM) was performed to compare survival outcomes of solid and part-solid lesions, in order to determine the prognostic value of GGO component. Cox proportional hazard model was performed to identify significant prognostic factors for resected node positive lung adenocarcinoma.
Results
About 5.1% (34 of 669) resected node-positive lung adenocarcinoma presented as part-solid nodules on CT images in this cohort. The median nodule size on CT of 34 part-solid lesions was 31mm (range: 15-68mm), median solid component size on CT was 24mm (range: 12-62mm), and median consolidation/tumor ratio was 0.8 (range: 0.64–0.95). After 1:4 PSM, 136 patients and 34 patients were matched from solid and part-solid groups. No significant difference in either RFS (P = 0.71) or OS (P = 0.82) were found between solid and part-solid groups. Multivariable Cox regression pN stage as the strongest prognostic factors for RFS and OS. GGO component was not an independent prognostic factor toward either RFS [P = 0.753, HR(95%CI) = 0.93(0.59–1.46)] or OS [P = 0.528, HR(95%CI) = 1.19(0.69–2.05)].
Conclusion
A minority of resected node-positive lung adenocarcinoma present as GGO component on CT. The presence of GGO component does not predict better prognosis in node-positive lung adenocarcinoma.
Transport and inhibition of the sphingosine-1-phosphate exporter SPNS2
Authors: Sauer David , Li Huanyu , Pike Ashley , Chang Yung-Ning , Prakaash Dheeraj , Gelova Zuzana , Stanka Josefina , Moreau Christophe , Scott Hannah , Wunder Frank , Wolf Gernot , Scacioc Andreea , McKinley Gavin , Batoulis Helena , Mukhopadhyay Shubhashish , Garofoli Andrea , Pinto-Fernandez Adan , Kessler Benedikt , Burgess-Brown Nicola , Štefanic Saša , Wiedmer Tabea , Dürr Katharina , Puetter Vera , Ehrmann Alexander , Khalid Syma , Ingles-Prieto Alvaro , Superti-Furga Giulio 
Published: November 2023

Abstract

Sphingosine-1-phosphate (S1P) is a signaling lysolipid critical to heart development, immunity, and hearing. Accordingly, mutations in the S1P transporter SPNS2 are associated with reduced white cell count and hearing defects. SPNS2 also exports the S1P-mimicking FTY720-P (Fingolimod) and thereby is central to the pharmacokinetics of this drug when treating multiple sclerosis. Here, we use a combination of cryo-electron microscopy, immunofluorescence, in vitro binding and in vivo S1P export assays, and molecular dynamic simulations to probe SPNS2’s substrate binding and transport. These results reveal the transporter’s binding mode to its native substrate S1P, the therapeutic FTY720-P, and the SPNS2-targeting inhibitor 33p. Further capturing an inward-facing apo state, our structures illuminate the protein’s mechanism for exchange between inward-facing and outward-facing conformations. Finally, using these structural, localization, and S1P transport results, we identify how pathogenic mutations ablate the protein’s export activity and thereby lead to hearing loss.
The Significance of Filling Ultrasonography for Preoperative Staging of Colon Cancer Diagnosis: A Prospective Study
Authors: Feng Yingkai , Zhao Junmei , Fa Xinyu , Sun Xuan 
Published: November 2023

Abstract
Purpose
To investigate the value of filling ultrasonography in the preoperative staging diagnosis of colon cancer.
Methods
This study was a prospective, single-center, observational study. We performed CT examination and filling ultrasonography in 118 patients diagnosed with colon cancer by preoperative pathologic biopsy and compared the accuracy of filling ultrasonography and CT examination and the combination of the two examinations in the diagnosis of preoperative staging of colon cancer, using postoperative pathologic findings as the gold standard.
Results
In 118 patients, the overall diagnostic compliance rate of ultrasonography for T stage of colon cancer was 69.5% (86/118), of which the diagnostic compliance rate for T1-T2 stage, 75% (63/84) for T3 stage, and 63.6% (14/22) for T4 stage. the overall diagnostic compliance rate for lymph node metastasis of colon cancer was 68.6% (81/118), of which the diagnostic compliance rate for N0 was 81.7% (49/60), N1 59.6% (28/47), and N2 36.4% (4/11).The total diagnostic compliance rate of CT for the T-stage of colon cancer was 55.9% (68/118), of which the diagnostic compliance rate for the T1-T2 stage was 33.3% (4/12), 52.38% (44/84) for T3 stage and 90.9% (20/22) for T4 stage; the total diagnostic compliance rate of CT for lymph node metastasis of colon cancer was 39.8% (47/118), of which the diagnostic compliance rate was 28.3% (17/60) for N0, 44.6% (21/47) for N1 and 81.8% (9/11) for N2. By ROC analysis, ultrasonography was superior to CT for the diagnosis of T-stage (0.438 vs 0.243, P < 0.05) and N-stage (0.480 vs 0.231, P < 0.005).
Conclusion
Filling Ultrasound is valuable in the diagnosis of tumor characterization, localization, and preoperative staging, and the combined diagnosis with CT significantly improves the accuracy of colon cancer diagnosis and makes up for the shortcomings of the examination.
Isolation of KH2PO4 from crude glycerol purified using Acromonia aculleata charcoal as adsorbent
Authors: Freitas Milton Souza , Godinho João Victor , Nelson David Lee , Wentz Alexandre P. , Neri Claudio Roberto , Zanatta Lucas D. , Pessoa Fernando L. P. , Barbosa Sandro L. 
Published: November 2023

Abstract
Activated charcoal was prepared from Acrocomia aculeata (macaúba) endocarp by ZnCl2 activation. The charcoal was used for the adsorptive purification of pretreated crude glycerol (CG) and potassium dihydrogen phosphate (KH2PO4 or KDP) was formed by the addition of H3PO4 to the crude glycerol containing KOH, which was used as catalyst in the production of biodiesel and glycerol. The KH2PO4 was isolated and purified by recrystallization from aqueous solution. The KH2PO4 was characterized by X-Ray diffraction (XRD), thermal analysis (TGA/DTA) and spectrophotometry in the infrared region – FTIR. From 216.0 m of crude glycerol, 20.1038 g of purified KH2PO4 was obtained.
Impaired homeostasis of T follicular helper cells in elderly patients with COVID-19
Authors: Wang Yihan , Wang Qiu , He Furong , Qiao Nan , Li Xuejun , Wei Liqun , Sun Lingjin , Dai Weiqian , Li Ying , Pang Xueyang , Hu Jiayi , Huang Chuan , Yang Guangchen , Pang Chongjie , Hu Zhidong , Xing Man , Wan Chunxiao , Zhou Dongming 
Published: November 2023

Abstract
Background
In December 2022, the discontinuation of China’s dynamic zero policy resulted in a nationwide surge of Corona Virus Disease 2019 (COVID-19)-related cases and hospitalizations. We sought to probe the immune profile of the elderly with COVID-19 and explore the feasibility of a certain cell population as biomarker for risk stratification, which may provide foundation for the diagnosis and treatment of upcoming COVID-19 wave this winter. Our study recruited 57 elderly patients with acute COVID-19 and 27 convalescent donors. SARS-CoV-2-specific adaptive immunity including binding antibodies, neutralizing antibodies and T-cell responses were assessed across the COVID-19 severity spectrum.
Results
Patients with acute illness underwent impaired CD4+ T homeostasis, preferential loss of follicular helper T cell (Tfh) subsets including Tfh-em, Tfh-cm, Tfh1, Tfh2, Tfh17 and T follicular regulatory cells (TFR), which correlated with antibody production through different pathways, were observed. Severity of acute respiratory distress syndrome correlated with the degree of Tfh deficiency, which may act as biomarkers for risk stratification of elderly patients with COVID-19. Moreover, vaccination ameliorated Tfh and TFR deficiency and helped to promote NAb production.
Conclusion
The elderly had gone through severity-dependent CD4+-biased lymphopenia post SARS-CoV-2 infection, and vaccination contributed to ameliorate prognosis of them via alleviating the impairment degree of Tfh subsets.