bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combination platelet glycoprotein IIb/IIIa receptor and lepirudin administration during percutaneous coronary intervention in patients with heparin‐induced thrombocytopenia
Authors: Pinto Duane S. , Sperling Robert T. , Tu Thomas M. , Cohen David J. , Carrozza Joseph P. 
Published: January 2003

AbstractWe evaluated a combination therapy using glycoprotein IIb/IIIa receptor antagonism and direct thrombin inhibition in nine patients with heparin‐induced thrombocytopenia (HIT) undergoing 10 percutaneous coronary interventions (PCIs). In selected patients with HIT, the combination of a direct thrombin inhibitor, lepirudin, and abciximab, tirofiban, or eptifibatide appears to be a safe and effective anticoagulation strategy for PCI. Cathet Cardiovasc Intervent 2003;58:65–68. © 2003 Wiley‐Liss, Inc.
Endovascular treatment of aortic rupture during angioplasty for aortic in‐stent restenosis in aortoarteritis
Authors: Tyagi Sanjay , Rangesetty Umamahesh C. , Kaul Upkar A. 
Published: January 2003

AbstractAortic rupture during balloon angioplasty for in‐stent restenosis without attempting to overexpand it is a rarity. We report a case of a young woman with aortoarteritis who had aortic rupture during angioplasty for in‐stent restenosis. The balloon used was of the same diameter as the previously implanted stent and was completely within the stent during inflation. The disruption was successfully treated by percutaneous placement of a self‐expandable endovascular stent graft. Cathet Cardiovasc Intervent 2003;58:103–106. © 2003 Wiley‐Liss, Inc.
Neutral and Ionic Hydrogen Bonding in Schiff Bases
Authors: Dominiak Paulina M. , Grech Eugeniusz , Barr Gordon , Teat Simon , Mallinson Paul , Woźniak Krzysztof 
Published: February 2003

AbstractLow‐temperature, high‐resolution X‐ray studies of charge distributions in the three Schiff bases, the dianil of 2‐hydroxy‐5‐methylisophthaldehyde, 3,5‐dinitro‐N‐salicylidenoethylamine and 3‐nitro‐N‐salicylidenocyclohexylamine, have been carried out. These structures exhibit interesting weak interactions, including two extreme cases of intramolecular hydrogen bonds that are ionic N+H⋅⋅⋅O−and neutral OH⋅⋅⋅N in nature. These two types of hydrogen bond reflect differences in geometrical parameters and electron density distribution. At the level of geometry, the neutral OH⋅⋅⋅N hydrogen bond is accompanied by an increase in the length of the C(1)O(1) bond, opening of theipso‐C(1) angle, elongation of the aromatic CC bonds, shortening of the C(7)N(2) bond and increased length of the C(1)C(7) bond, relative to the ionic hydrogen bond type. According to the geometrical and critical point parameters, the neutral OH⋅⋅⋅N hydrogen bond seems to be stronger than the ionic ones. There are also differences between charge density parameters of the aromatic rings consistent with the neutral hydrogen bond being stronger than the ionic ones, with a concomitant reduction in the aromaticity of the ring. Compounds with the ionic hydrogen bonds show a larger double‐bond character in the CO bond than appears in the compound containing a neutral hydrogen bond; this suggests that the electronic structure of the former pair of compounds includes a contribution from a zwitterionic canonical form. Furthermore, in the case of ionic hydrogen bonds, the corresponding interaction lines appear to be curved in the vicinity of the hydrogen atoms. In the 3‐nitro‐N‐salicylidenocyclohexylamine crystal there exists, in addition to the intramolecular hydrogen bond, a pair of intermolecular O⋅⋅⋅H interactions in a centrosymmetric dimer unit.
Combinatorial Library of Low Molecular‐Weight Organo‐ and Hydrogelators Based on Glycosylated Amino Acid Derivatives by Solid‐Phase Synthesis
Authors: Kiyonaka Shigeki , Shinkai Seiji , Hamachi Itaru 
Published: February 2003

AbstractA combinatorial approach for the synthesis of supramolecular gelators as new organic materials is described herein. In the course of the development of a convenient and flexible solid‐phase synthesis of the artificial glycolipids, some of these compounds were accidentally found to act as low molecular‐weight gelators toward organic solvents. Using this combinatorial solid‐phase synthesis of glycosylated amino acetates, screening and optimization of low molecular‐weight organo/hydro‐gelators were efficiently carried out. We found that an N‐acetyl‐galactosamine‐appended amino acid ester (GalNAc‐aa) efficiently gelates a broad spectrum of organic solvents. More interestingly, some GalNAc‐aa derivatives displayed an excellent hydrogelation capability. Transmission electron microscopy, scanning electron microscopy, confocal laser scanning microscopy, and FT‐IR were used for characterization of the gel structure. It is indicated that supramolecular fibers supported by strong hydrogen‐bonding networks are entangled so that the resulting spaces can immobilize a number of solvent molecules effectively. In addition, the supramolecular hydrogel consisting of GalNAc‐suc‐glu(O‐methyl‐cyc‐pentyl)2 is stable even under high salt concentrations probably due to its nonionic character and as a result, a native protein is successfully entrapped in the gel matrix without denaturation.
Neurochemical comparison of synaptic arrangements of parvocellular, magnocellular, and koniocellular geniculate pathways in owl monkey (Aotus trivirgatus) visual cortex
Authors: Shostak Yuri , Ding Yuchuan , Casagrande Vivien A. 
Published: January 2003

AbstractAs in other primates, the lateral geniculate nucleus (LGN) of owl monkeys contains three anatomically and physiologically distinct relay cell classes, the magnocellular (M), parvocellular (P), and koniocellular (K) cells. M and P LGN cells send axons to the upper and lower tiers of layer IV, and K cells send axons to the cytochrome oxidase (CO) blobs of layer III and to layer I of primary visual cortex (V1). Our objective was to compare the synaptic arrangements made by these axon classes. M, P, and K axons were labeled in adult owl monkeys by means of injections of wheat germ agglutinin‐horseradish peroxidase into the appropriate LGN layers. The neurochemical content of both pre‐ and postsynaptic profiles were identified by postembedding immunocytochemistry for γ‐aminobutyric acid (GABA) and glutamate. Our key finding is that the synaptic arrangements made by M, P, and K axons in owl monkey exhibit more similarities than differences. They are exclusively presynaptic, contain glutamate and form asymmetric synapses mainly with glutamate‐positive dendritic spines. The majority of the remaining axons synapse with glutamatergic dendritic shafts. There are also differences between LGN pathways. M and P terminals are significantly larger and more likely to make multiple synapses than K axons, although M and P axons do not differ from each other in either of these characteristics. Of interest, a larger percentage of M and K axons than P axons make synapses with GABAergic dendritic shafts. Cells directly postsynaptic to M and K axons are known to exhibit orientation selectivity and, in some cases, direction selectivity. Cells postsynaptic to P axons do not show these properties, but instead tend to reflect their LGN inputs more faithfully; therefore, it is possible that these physiologic differences seen in the cortical cells postsynaptic to different LGN pathways reflect the differential involvement of inhibitory circuits. J. Comp. Neurol. 456:12–28, 2003. © 2002 Wiley‐Liss, Inc.
Acquisition of neuronal and glial markers by neural crest–derived cells in the mouse intestine
Authors: Young Heather M. , Bergner Annette J. , Müller Thomas 
Published: January 2003

AbstractEnteric neurons and glia arise from the neural crest. The phenotype of crest‐derived cells was examined as they differentiated into neurons or glia in the mouse small and large intestine. Previous studies have shown that undifferentiated enteric crest–derived cells are Phox2b+/Ret+/p75+/Sox10+, and at embryonic day (E) 10.5, about 10–15% of the crest‐derived cells in the small intestine have started to differentiate into neurons. In the current study, by E12.5 and E14.5, about 25% and 47%, respectively, of Phox2b+ cells in the small intestine were immunoreactive to the pan‐neuronal protein, ubitquitin hydrolase (PGP9.5), and the percentage did not change dramatically from E14.5 onward. The differentiation of crest‐derived cells into neurons in the colon lagged behind that in the small intestine by several days. Differentiating enteric neurons showed high Ret, low p75, and undetectable Sox10 immunostaining. Glial precursors were identified by the presence of brain‐specific fatty acid binding protein (B‐FABP) and detected first in the fore‐ and rostral midgut at E11.5. Glial precursors appeared to be B‐FABP+/Sox10+/p75+ but showed low Ret immunostaining. S100b was not detected until E14.5. Adult glial cells were B‐FABP+/Sox10+/p75+/S100b+. A nucleic acid stain (to identify all ganglion cells) was combined with immunostaining for PGP9.5 and S100b to detect neurons and glial cells, respectively, in the postnatal intestine. At postnatal day 0, fewer than 5% and 10% of cells in myenteric ganglia of the small and large intestine, respectively, were neither PGP9.5+ nor S100b+. Because some classes of neurons are not present in significant numbers until after birth, the expression of PGP9.5 by developing enteric neurons appeared to precede the expression of neuron type–specific markers. J. Comp. Neurol. 456:1–11, 2003. © 2002 Wiley‐Liss, Inc.
Ontogeny of calcitonin receptor mRNA and protein in the developing central nervous system of the rat
Authors: Tolcos Mary , Tikellis Christos , Rees Sandra , Cooper Mark , Wookey Peter 
Published: January 2003

AbstractIn this study, the expression of receptors for calcitonin (CTR), the CTR C1a and C1b isoforms, was investigated during development of the fetal rat central nervous system (CNS) by using in situ hybridization and immunohistochemistry. Coincident expression with both techniques was evident. Immunohistochemical evidence for the expression of the C1a isoform alone was found. Expression was first observed at embryonic day 12/13 (E12/E13) within and adjacent to the ventricular zones known to include primary matrices of proliferation, in regions of the preoptic area, anterior and posterior hypothalamus, anterior and posterior pons, medulla, and spinal cord. At later times, with the decline in the density of immunoreactivity at these loci (E15), expression in primary matrices was found later at distinct loci within the ventricular zones of cerebellum (E17), and at E19, the tectum, lateral ventricle, and cortical subplate. By E19, the density of staining had increased and was widespread throughout the expanding CNS. In the rostral domains, moderate to high density was found in the external plexiform layer; the medial preoptic area and nucleus; the ventromedial, dorsomedial, and arcuate hypothalamic nuclei; and the lateral and posterior hypothalamic areas. In the midbrain, similar levels of expression were noted in the central nucleus of raphe; the deep mesencephalic, dorsal raphe, and laterodorsal tegmental nuclei; and the ventral periaqueductal gray. In the pons, positive loci included the locus coeruleus and the gigantocellular and pontine reticular nuclei. In the medulla, high expression was evident in the gigantocellular, intermediate, magnocellular, and medullary reticular, spinal trigeminal and cuneate nuclei; and the nucleus tractus solitarius. In the spinal cord, moderate to high density of staining was found in the ventral, dorsal, and lateral horns, and in the ventral, dorsal, and cuneate funiculi. On the other hand, transitory expression was found in the diagonal band, bed nucleus of the stria terminalis, amygdala, and the lateral mamillary and anterobasal nuclei of the hypothalamus. These studies indicate a role for CTR in the activation of some premigratory neuroblasts in the CNS as well as a possible role later in an undefined function associated with mature neurons of particular nuclei. J. Comp. Neurol. 456:29–38, 2003. © 2002 Wiley‐Liss, Inc.
Expression and distribution of tuberoinfundibular peptide of 39 residues in the rat central nervous system
Authors: Dobolyi Arpad , Palkovits Miklós , Usdin Ted Björn 
Published: January 2003

AbstractTuberoinfundibular peptide of 39 residues (TIP39) has been recently purified and identified as a selective ligand for the parathyroid hormone 2 receptor. As a next step toward understanding its functions, we report the expression and distribution of TIP39 in the rat central nervous system. In situ hybridization histochemistry and immunocytochemistry revealed TIP39‐containing cell bodies in three distinct areas. The major one comprises the subparafascicular area posterior through the intralaminar nucleus of the thalamus; a second is the medial paralemniscal nucleus at the pontomesencephalic junction; and a third is in the dorsal and dorsolateral hypothalamic areas, which contained a few, scattered cell bodies. We found, in contrast to the highly restricted localization of TIP39‐containing cell bodies, a much more widespread localization of TIP39‐containing fibers. The highest density of fibers was observed in limbic areas such as the septum, the amygdala, and the bed nucleus of the stria terminalis; in areas involved in endocrine regulation, such as the hypothalamic dorsomedial, paraventricular, periventricular, and arcuate nuclei; in auditory areas, such as the ectorhinal and temporal cortices, inferior colliculus, medial geniculate body, and some of the nuclei of the superior olivary complex; and in the dorsolateral funiculus of the spinal cord. The localization of TIP39‐containing nuclei and fibers provides an anatomical basis for previously demonstrated endocrine and nociceptive effects of TIP39 and suggests additional functions for TIP39, one apparent candidate being the regulation of auditory information processing. J. Comp. Neurol. 455:547–566, 2003. Published 2002 Wiley‐Liss, Inc.
Follicular thyroid lesions coexisting with Hashimoto's thyroiditis: Incidence and possible sources of diagnostic errors
Authors: Kollur Sharanamma M. , El Sayed Salah , El Hag Imad Abdien 
Published: January 2003

AbstractOur objectives were to study the types and incidence of thyroid follicular lesions coexisting with Hashimoto's thyroiditis (HT), the pitfalls in their cytodiagnosis, and the effect on management. All cases of HT diagnosed by fine‐needle aspiration (FNA) and/or histology over a 7‐yr period were retrospectively studied. HT coexisted with follicular adenoma (FA) in 6 cases, follicular variant of papillary carcinoma (FVPC) in 1 case, and goitrous nodule (GN) in 2 cases. The overall incidence rates of thyroid neoplasm and goitrous nodules coexistent with HT were 15% and 3.5%, respectively. A preoperative FNA diagnosis was available in 10 histologically proven cases of HT. A false‐positive diagnosis of follicular neoplasm (FN) that led to unnecessary thyroidectomies was given in 3 cases. In 2 of these, the cytological diagnosis was HT with the possibility of coexisting FN, and in the third case, the cytological finding of HT was misinterpreted as FN. The main causes of these diagnostic pitfalls were the presence of hyperplastic follicular cells with nuclear pleomorphism, a paucity of lymphoid cells in burned‐out HT, and lack of ones exposure. Nuclear pleomorphism was observed in none of the follicular adenomas. FNA diagnosed accurately the coexisting lesions in 6 cases; 3 FA, 1 FVPC, and 2 GN, but it did not sample HT. In one case, FNA diagnosed correctly both HT and the coexisting FA. Therefore, the presence of a coexistent neoplasm or goitrous nodule reduced the chances of sampling HT by 85.7%, with no false‐negative results. Indeed, aspiration on and around the thyroid nodule helps in sampling HT. However, HT may dominate the smear and obscure neoplasia. This can be avoided if the procedure is performed by the pathologist and the aspiration is done on the nodule only. The overlapping cytological features of FN and HT were the main causes of false‐positive results. This can be reduced by avoiding the diagnosis of FN in the presence of follicular‐cell pleomorphism and/or moderate to excessive numbers of lymphoid cells, provided proper aspiration technique is maintained. Diagn. Cytopathol. 2003;28:35–38. © 2003 Wiley‐Liss, Inc.
Perineal nodule due to enterobiasis: An aspiration cytologic diagnosis
Authors: Kumar Neeta , Sharma Prashant , Sachdeva Ritesh , Jain Shyama 
Published: January 2003

AbstractSubcutaneous swelling due to Enterobious vermicularis infection is rare. Their presence in perineal subcutaneous tissue is unusual and a clinical curiosity. A case of subcutaneous nodule in the perineum due to E. vermicularis infection diagnosed on fine‐needle aspiration is described. Tissue reaction was granulomatous with neutrophils and eosinophils. Lack of familiarity with the morphology of parasites or its eggs, degeneration, and inadequate sampling may make the diagnosis difficult. This case report provides the morphologic clues to cytopathologists toward rendering a diagnosis and alerts them to the possibility of parasitic infection in unusual locations. Diagn. Cytopathol. 2003;28:58–60. © 2003 Wiley‐Liss, Inc.
Expression of Sox9 in granulosa cells lacking the estrogen receptors, ERα and ERβ
Authors: Dupont Sonia , Dennefeld Christine , Krust Andrée , Chambon Pierre , Mark Manuel 
Published: January 2003

AbstractOvaries from adult mice lacking both estrogen receptors ERα and ERβ (ERαβKO mice) contain abnormal cells sharing morphologic features with Sertoli cells, which are located mainly in the interstitial compartment. We show here that these cells express the Sertoli cell markers TIF1β, TIF2, and Sox9. In ERαβKO ovaries, Sox9 is expressed by granulosa cells before the morphologic appearance of Sertoli cells, but neither by granulosa cell precursors nor by non‐Sertolian interstitial cells. These findings suggest that functional Sertoli cells can transdifferentiate from mature granulosa cells devoid of estrogen receptors as a result of Sox9 expression. © 2002 Wiley‐Liss, Inc.
Transitions in the model of epithelial patterning
Authors: Přibyl Michal , Muratov Cyrill B. , Shvartsman Stanislav Y. 
Published: January 2003

AbstractWe analyze pattern formation in the model of cell communication in Drosophila egg development. The model describes the regulatory network formed by the epidermal growth factor receptor (EGFR) and its ligands. The network is activated by the oocyte‐derived input that is modulated by feedback loops within the follicular epithelium. We analyze these dynamics within the framework of a recently proposed mathematical model of EGFR signaling (Shvartsman et al. [2002] Development 129:2577–2589). The emphasis is on the large‐amplitude solutions of the model that can be correlated with the experimentally observed patterns of protein and gene expression. Our analysis of transitions between the major classes of patterns in the model can be used to interpret the experimentally observed phenotypic transitions in eggshell morphology in Drosophila melanogaster. The existence of complex patterns in the model can be used to account for complex eggshell morphologies in related fly species. © 2002 Wiley‐Liss, Inc.
Effect of olive paste kneading process time on the overall quality of virgin olive oil
Authors: Ranalli Alfonso , Pollastri Luciano , Contento Stefania , Iannucci Emilia , Lucera Lucia 
Published: February 2003

AbstractThe influence of the olive paste malaxation time on the composition and the industrial output of oil was investigated. To this purpose, three Italian olive varieties (Leccino, Dritta, Caroleo) were processed with a centrifugal system for six malaxation periods (0, 15, 30, 45, 60 and 75 min). The concentrations of the majority of the oil constituents changed during the malaxation. However, these changes were not significant for all of them: the contents of β‐carotene, the major xanthophylls, chlorophylls a and b, pheophytins a and b in the oils increased progressively with increasing malaxing times, whereas the contents of simple and hydrolysable phenols (secoiridoid derivatives), o‐diphenols and total phenols decreased. A significant increase in total volatiles and green volatiles of the lipoxygenase cascade (C6 aldehydes, C6 alcohols, C5 alcohols and C5 carbonyls) was detected. An opposite trend was observed for the green C6 esters. As a result, the global analytical quality, flavour, aroma and shelf‐life of the oils were negatively affected. The oil yield increased substantially up to 45 min of paste malaxation times. Beyond 60 min, the yields tended to decrease.
Evaluation of Structure, Nucleation and Growth Mechanism of Electropolymerized Polypyrrole on Highly Oriented Pyrolytic Graphite Electrode
Authors: Hwang Bing‐Joe , Santhanam Raman , Lin Yi‐Liang 
Published: February 2003

AbstractChronoamperometry (i–t) and atomic force microscopy (AFM) were used to study the nucleation and growth mechanism of electropolymerized polypyrrole formed on highly oriented pyrolytic graphite (HOPG) substrate surface. From i–t measurements it was found that the nucleation and growth mechanism was a 3‐D progressive after nuclei overlapping. However, before nuclei overlapping, it was not clear whether the mechanism was 2‐D instantaneous or 3‐D progressive. This ambiguity in the nucleation and growth mechanism was confirmed as 3‐D progressive from the results of AFM measurements. In addition to nucleation and growth mechanism, possible models were proposed for different structures observed on the AFM image of polypyrrole, obtained from very early stages of polymerization.
New mutations and protein variants of NBS1 are identified in cancer cell lines
Authors: Tessitore Alessandra , Biordi Leda , Flati Vincenzo , Toniato Elena , Marchetti Paolo , Ricevuto Enrico , Ficorella Corrado , Scotto Luigi , Giannini Giuseppe , Frati Luigi , Masciocchi Carlo , Tombolini Vincenzo , Gulino Alberto , Martinotti Stefano 
Published: January 2003

AbstractAlterations of the NBS1 gene are responsible for Nijmegen breakage syndrome (NBS), which is characterized by chromosomal instability, radiosensitivity, and cancer predisposition. NBS1 protein (Nibrin) is part of a molecular complex (NBS1– MRE11A–RAD50) that is functionally involved in DNA double‐strand–break repair. Defects in recombination or in repair mechanisms at the level of DNA breakage can lead to chromosomal aberrations, genetic instability, as well as cancer predisposition syndromes (i.e., NBS, ataxia‐telangiectasia, Bloom syndrome). In this study, we examined 20 cancer cell lines to evaluate the potential involvement of NBS1 in tumoral pathogenesis. Three different mutations, generating truncated or aberrant NBS1 transcripts, were identified at the level of NBS1 mRNA. In addition, two shorter NBS1 protein variants were detected in two cell lines. These data suggest a possible involvement of NBS1 in tumor development. © 2003 Wiley‐Liss, Inc.
Intravenous leiomyomatosis is characterized by a der(14)t(12;14)(q15;q24)
Authors: Dal Cin Paola , Quade Bradley J. , Neskey David M. , Kleinman Michael S. , Weremowicz Stanislawa , Morton Cynthia C. 
Published: January 2003

AbstractIntravenous leiomyomatosis (IVL) is a rare smooth‐muscle proliferation that is of special interest because of its quasi‐malignant behavior. Our finding of a specific chromosomal aberration, a der(14)t(12;14)(q15;q24), in a second case of IVL suggests that it may be characteristic of IVL. We propose that IVL arises from a uterine leiomyoma with a t(12;14)(q15;q24). The presence of an extra copy of 12q15‐qter and/or loss of 14q24‐qter may be a critical genetic event(s) leading to intravascular intrusion and proliferation. © 2003 Wiley‐Liss, Inc.