bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early life adversity and White Matter Microstructural Organization - a systematic review
Authors: Mitchell Orla , Roddy Darren W , Connaughton Michael 
Published: February 2025

AbstractEarly life adversity, defined as exposure to stressful events during childhood, is a significant risk factor for the development of psychiatric disorders. Diffusion tensor imaging studies employing tract-based spatial statistics have shown microstructural abnormalities in white matter among individuals exposed to early life adversity; however, robust conclusions are yet to be drawn. This systematic review synthesizes findings of previous tract-based spatial statistics studies to identify the white matter alterations in adult brains exposed to early life adversity, in papers with methodological consistency. The literature search (April 2024) was conducted to identify tract-based spatial statistics studies that compared diffusion metrics between adults exposed to early life adversity and adults not. Embase, Pubmed, and PsycInfo were searched, retrieving 2458 articles. Following deduplication, 1739 titles and/or abstracts were screened. 1699 articles were excluded, and 40 full texts were reviewed. Seven articles, reporting on 764 subjects, met the inclusion criteria and were included in the narrative synthesis. Compared to controls, adults exposed to early life adversity showed lower fractional anisotropy values in white matter tracts of the limbic and visual processing systems, specifically the anterior thalamic radiation, inferior longitudinal fasciculus, corona radiata, uncinate fasciculus, inferior fronto-occipital fasciculus, and cingulum bundle. This systematic review highlights that early life adversity may underlie emotional dysregulation and contribute to an increased risk of psychopathology in later life and explores the potential neurobiological mechanisms that underpin these structural changes. Understanding these associations is crucial for developing targeted interventions aimed at mitigating the long-term impact of early life adversity.
Inferring binding rates from enzymatic turnover time statistics
Authors: Singh Divya , Urbakh Michael , Reuveni Shlomi 
Published: February 2025

AbstractWe present a method to extract enzyme-substrate binding rates from observations of enzymatic turnover times. Our approach involves segregating the short-time statistics of the turnover time distribution and inferring from it the binding rate in a general and robust manner. Beyond determining binding rates, the approach developed herein also allows us to infer: (i) the fertile collision probabilityp, i.e., the probability that a collision between an enzyme and a substrate will result in the formation of an enzyme-substrate complex; and (ii) the fertile catalysis probabilityϕcat, i.e., the probability that the enzyme-substrate complex will lead to the formation of a product. It has long been known thatpϕcat≪ 1, indicating that most enzymes operate far from maximal efficiency, yet separating the contributions of fertile binding and catalysis was not possible in lieu of direct binding rate measurements. Our method overcomes this limitation by enabling precise inference of binding rates from turnover times, which in turn opens the door for a more detailed understanding of enzymatic efficiency.
The mechanics of a continuous self-assemblingsurface-layer aids cell division in an archaeon
Authors: Foo Sherman , Caspy Ido , Cezanne Alice , Bharat Tanmay A.M. , Baum Buzz 
Published: February 2025

AbstractThe surface layer or “S-layer” is a planar lattice of glycosylated proteins that coats a wide range of archaea and bacteria instead of a classical cell wall or capsular polysaccharides, insulating them from the extracellular space and providing the cell membrane with physical support. Although the S-layer’s role as a mechanical support for the membrane might be expected to hinder cell division, we show that in rapidly dividingSulfolobus acidocaldariuscells, the S-layer protein SlaA self-assembles into flexible lattice that helps flattens the cytokinetic furrow to accelerate ESCRT-III dependent cell division - a role that is especially important under conditions of mechanical stress. Taken together, these results generated using mutational analysis, live and fixed cellular imaging, along with electron cryomicroscopy, define the rules governing S-layer self-assembly and show how the mechanical properties of flexible lattice coats can enhance membrane functions to both physically support a cell and help to drive ESCRT-III dependent cell division.Significance statementThe bounding membrane of a cell must be protected from environmental insults. In many bacteria and archaea that lack a cell wall, this is achieved by an enveloping S-layer. By virtue of its rigid, planar structure, the S-layer is also expected to act as a barrier to the cell shape changes required for cytokinesis. In this study of the S-layer lattice in the archaeonSulfolobus acidocaldariushowever, we demonstrate a role for this self-assembling mechanical support in re-shaping the cytokinetic furrow as an aid to ESCRT-III-mediated division. In showing how mechanically active and passive structures can work together to give rise to mechanically stable cells that can divide, this reconciles what seems like a trade-off between resilience and flexibility.
Programmedin vivoexcision of prophage Φ13 is an essential timing device for theStaphylococcus aureusinfectious process
Authors: Poupel Olivier , Kenanian Gérald , Touqui Lhousseine , Abrial Charlotte , Msadek Tarek , Dubrac Sarah 
Published: February 2025

ABSTRACTMobile genetic elements play an essential part in the infectious process of major pathogens, yet the role of prophage dynamics inStaphylococcus aureusremains poorly understood. Here we studied the impact of the Φ13hlb-converting prophage, whose integration inactivates thehlbβ-toxin gene, on staphylococcal pathogenesis. We showed that prophage Φ13 is lost in approximately half the bacterial population during the course of infection. Inactivation of the Φ13intrecombinase gene, essential for insertion/excision, locked the prophage in the bacterial chromosome, leading to a significant loss of virulence in a murine systemic infection model. In contrast, the non-lysogen strain (ΔΦ13), where thehlbbeta-hemolysin gene is reconstituted, displayed strongly increased virulence. Accordingly, histopathological analyses revealed more severe nephritis in mice infected with bacteria lacking prophage Φ13 (ΔΦ13), in contrast to infection with the parental strain and particularly with the Δintmutant, which led to less severe renal lesions. Cytokine induction in a human neutrophil model showed significantly increased IL-6 expression following infection with the beta-hemolysin producing strain (ΔΦ13). Our results indicate that programmedin vivoexcision of the Φ13 prophage is essential for progression of theStaphylococcus aureusinfectious process, since early excision rapidly kills the host whereas the absence of excision significantly lowers staphylococcal virulence.IMPORTANCEThis study highlights prophage Φ13 excision as a critical factor inS. aureuspathogenesis, influencing infection outcomes by balancing rapid host killing with reduced bacterial virulence. This mechanism may represent a bet-hedging strategy in genetic regulation, resulting in a mixed bacterial population capable of rapidly switching between two processes: bacterial colonization and host damage. Unraveling this dynamic opens new possibilities for developing targeted therapies to disrupt or modulate prophage activity, offering a novel approach to mitigatingS. aureusinfections.
Axon collateral pattern of a sparse locus coeruleus norepinephrine neuron in mouse cerebral cortex
Authors: Wu Yu-Ming , Hung Wei-Chen , Chang Yun , Min Ming-Yuan , Yang Hsiu-Wen 
Published: February 2025

AbstractThe locus coeruleus (LC) contains predominantly norepinephrine (NE) neurons with widespread axonal projections throughout the brain and plays a critical role in the control of behavior, particularly arousal. Historically, the LC-NE system was thought to perform this function by releasing NE uniformly throughout most brain regions. However, recent evidence suggests that the cortical projections of the LC are modularly organized, allowing coordination of diverse and sometimes opposing functions, such as fear memory formation and extinction. However, many details remain unclear, and answers require data from axon collaterals of sparse neurons. We modified a viral tracing protocol using a dual recombinase system to trace the axonal collaterals of sparse LC neurons projecting to the cingulate cortex (CgC). Our results show that even a small number of LC neurons still have broad cortical projections, but the pattern is not uniform. There are a few major projections with dense fibers and numerous minor projections with sparse innervation. Notably, the major projections, including the rostrosplenial cortex, dorsal hippocampus, somatosensory cortex, and CgC (the injection site for retrograde viral labeling), are functionally related and may coordinate goal-directed navigation. In conclusion, our results suggest that a single LC neuron projects divergently and non-uniformly to the cortex, with major projections coordinating related cortical areas to regulate specific functions, while minor projections appear less functionally specific. It’s likely that each LC-NE neuron plays a role in maintaining arousal through the minor projections.
In silicoclassification and identification co-purified protein complexes yield new structures and multiple MSP assembly states
Authors: Zhang Qingyang , Murthy Abhinandan Venkatesha , Mim Carsten 
Published: February 2025

AbstractNative protein complexes have garnered interest as targets for structural dissemination. Cryogenic electron microscopy (cryoEM) with its ability to image protein mixtures is the most promising tool to enable structural proteomics. Additionally, image processing has evolved to deal with conformational and compositional heterogeneity. Integrative approaches, namely mass spectrometry in conjunction with cryoEM have made it possible to characterize and identify complex mixtures. However, this comes at a cost of generating models and interpreting mass spectra. Here we present a modified approach that only requires electron micrographs and a computer for unsupervised model building and protein identification. We were able to identify co-purified membrane proteins, resulting in a novel structure and unexpected nanodisc assemblies, which imply direct interaction between membrane proteins and membrane scaffolding proteins.
Maturation-dependent complement production and C3 processing in human retinal pigment epithelium cells
Authors: Schikora Juliane , Nickel Antonia , Bergert Jasmin , Hähnel Rike , Dort Aaron , Schayan-Araghi Stella Y. , Banerjee Pratiti , Wolf Hannah N. , May-Simera Helen , Pauly Diana 
Published: February 2025

AbstractInduced pluripotent stem cell-derived retinal pigment epithelial (iPSC-RPE) cells, closely resembling healthy RPE, offer valuable insights for retinal disease modelling. This study evaluates immature and mature phenotypes of iPSC-RPE and ARPE-19 cells, comparing RPE characteristics, cell-associated complement profiles, and TGF-β1-mediated stress responses across transcript expression, secretion, and protein levels. Statistical analyses were performed using independent meanst-test and Wilcoxon rank-sum test. Mature iPSC-RPE cells exhibited characteristic RPE-morphology, with elevated secretion of complement components (C3, FH/FHL-1, FI), unique FB secretion, and apical complement localisation. Intracellular C3 processing revealed cleavage products different from known blood-derived C3 fragments and showed maturation-dependent differences; only mature iPSC-RPE cells secreted active C3 forms (C3(H2O), C3a) and exhibited the intact C3 β-chain. Mature ARPE-19 and iPSC-RPE cells demonstrated resistance to TGF-β1 treatment, which reduced complement secretion without affecting C3a release. In conclusion, ARPE-19 and iPSC-RPE cells demonstrated local production of complement components and maturation-dependent intracellular processing of C3 into active forms. These findings highlight the impact of RPE maturation on local C3 activation, providing a basis for future studies on C3 functionality in the RPE and its potential pathological effects.Impact statementCell state-dependent local processing of complement C3 in retinal pigment epithelium cells generates active C3 forms that may evade anti-C3 therapies, influencing age-related macular degeneration and treatment outcomes.Funding statementThis project has received funding from the PRO RETINA, Amberg, Germany, under grant agreement number “Pro-Re/Projekt/Schikora – Pauly.05-2022”, and Deutsche Forschungsgemeinschaft (DFG), under grant agreement number “498244102 (MA 6139/5-1)“. Open access funding provided by the Open Access Publishing Fund of University Marburg. Funding sources were not involved in study design, data collection and interpretation, or the decision to submit the work for publication.Graphical abstract
Cost-sensitive feature selection for multi-label classification: multi-criteria decision-making approach
Authors: Mohanrasu S.S. , Phan Le Thi , Rajan Rakkiyappan , Manavalan Balachandran 
Published: February 2025

PurposeIn multi-label classification, selecting the most relevant features is crucial for enhancing predictive performance and reducing computational complexity. Real-world scenarios often involve significant costs in data acquisition, including time, financial and computational resources. However, most existing feature selection methods overlook the associated costs.Design/methodology/approachMulticriteria decision-making (MCDM) has emerged as a powerful tool for addressing complex problems involving multiple, often conflicting criteria. This study proposes a novel cost-sensitive multi-label feature selection method that fuses feature importance with feature cost within an MCDM framework. The proposed method transforms a cost-sensitive multi-label feature selection problem into an MCDM problem by leveraging mutual information. Furthermore, the data were converted into Fermatean fuzzy sets, and the Fermatean fuzzy simple weighted sum product (WISP) method was employed to rank features based on their relevance to labels and associated costs.FindingsExtensive experiments conducted on ten benchmark datasets against five evaluation metrics demonstrated the superiority of the proposed method in selecting relevant features while minimizing costs and consistently outperforming existing methods.Originality/valueUnlike existing methods that integrate costs through penalties and select features via a greedy search, the proposed approach adopts an MCDM-based strategy for feature ranking. This method aims to achieve globally optimal outcomes by balancing the trade-offs between conflicting objectives, marking a significant advancement over existing techniques.Graphicalabstract
Impact of time-based variability on planning reliability in construction projects: analysis of production management systems
Authors: León William , Guevara Jose , Garcia-Lopez Nelly P. 
Published: February 2025

PurposeThis research article proposes a quantitative method for analyzing planning reliability and risk evaluation in construction projects through probabilistic numerical simulation models. The aim is to assess the combined time-based variability of activities across the project and its impact on planning reliability in various production management systems.Design/methodology/approachThe study examines different methods for comparing the impact of production management strategies, specifically standardization and lean construction practices, on project reliability. The article introduces Monte Carlo, importance sampling and first-order-second moment methods as quantitative tools for decision-making and continuous improvement in construction projects.FindingsThe utilization of multiple numerical simulation models demonstrates how the variability in activity durations directly affects planning reliability, leading to uncertainty and increased risk in construction projects. The study highlights the significance of lean construction principles and industrialized processes, such as standardization of processes and construction methods, in reducing variability and improving the average duration of activities. Furthermore, it quantifies the differential impact of various production management systems on project performance and planning reliability.Research limitations/implicationsThis study is based on a limited number of case studies, which may affect the generalizability of the findings. The results are specific to the construction projects analyzed in Colombia. Expanding the research to include a broader range of projects will improve the applicability of the findings. Developing more comprehensive numerical models can also enhance understanding of planning reliability across different construction management systems.Originality/valueCurrently, there are limited quantitative methods available for project managers to evaluate the impact of industrialized construction practices and production management strategies on planning reliability as well as quantifying the associated risk level in project delays. Consequently, the adoption of innovative construction methods is often limited to a few pilot projects, as managers lack numerical evidence to support the implementation of new methodologies that require changes in traditional operational practices.
Metaverse adoption and its implications for entrepreneurial innovation management: the influence of Gen Z’s perception of innovation, privacy and trust
Authors: Hernández-Tamurejo Álvaro , Fernández-Fernández María , González-Padilla Paula 
Published: February 2025

PurposeThis study investigates Generation Z’s perceptions of entrepreneurial innovation through the Metaverse, including user perceptions of privacy and trust, and their impact on the acceptance of Metaverse technology.Design/methodology/approachUsing a quantitative approach to examine relationships between the targeted factors, this study employs a structural equation modeling (SEM) based on the technology acceptance model extended with the variables related to privacy, trust and product innovation. The data were collected through a survey distributed to a representative sample of Gen Z individuals.FindingsThe results reveal that user perception of trust and product innovation positively influences attitudes toward using the Metaverse and the behavioral intention to use it. However, while privacy risk is found to significantly influence trust, it does not affect user attitudes or intention to use the Metaverse.Practical implicationsThe results of this study provide useful insights for enterprises, raising considerations to maximize the innovative potential of the Metaverse in the current business ecosystem. The understanding of Gen Z’s perceptions can help enterprises to better adapt their innovation management practices so as to effectively engage this demographic, ensuring the successful adoption of Metaverse technologies.Originality/valueThis study is among the first empirical investigations on the impact of Gen Z on innovation management through the Metaverse, which is an emerging and increasingly important area.
Employee moonlighting: the role of human resource management practices, perceived employability and self-direction value
Authors: Tran Huy Phuong 
Published: February 2025

PurposeMoonlighting has become more popular today in many fields such as IT, healthcare and education. This research investigates the direct association between high-performance work system (HPWS) and employee moonlighting (EM) and the mediating effect of perceived employability (PE) in the above relationship. Moreover, the personal value of self-direction is included as a moderator between PE and moonlighting.Design/methodology/approachThis research follows a quantitative design. An online questionnaire survey is used to collect data. Data from 456 Vietnamese employees were analyzed with partial least square-structural equation modelling (PLS-SEM) technique.FindingsHPWS is directly linked with EM. Perceived external employability fully mediates the above relationship. In addition, the personal value of self-direction intensifies the positive relationship between PE and EM.Research limitations/implicationsThe research suggests that HPWS can exert its influence on EM via both the universal and the critical pathways. Employees who perceive they have the ability to perform well in other jobs may decide to moonlight. Also, employees who find their jobs insecure under the coverage of HPWS may engage in moonlighting for security reasons. The implementation improves the employees’ PE which may encourage them to hold more jobs. In addition, attention should be paid to the personal value of the employees.Originality/valueThis study offers new insight into the HPWS and EM literature because it suggests that management practices can significantly influence EM, in addition to financial and security factors. Moreover, this study explores the indirect impact of PE in the HPWS–EM linkage. Finally, the moderation role of self-direction value represents a novelty.
Prioritizing fire risk factors in historic urban cores of India: an analytic hierarchy process approach
Authors: Goswami Shipra , Kolte Rushikesh , Kumar Ashwani , Pipralia Satish 
Published: February 2025

Purpose
This study aims to present a comprehensive examination of fire risk components in the historic core areas of Indian cities through the analytic hierarchy process (AHP) lens. Urban fire incidents pose significant threats to public safety and infrastructure, necessitating a systematic evaluation of key risk factors.

Design/methodology/approach
This study presents a comprehensive examination of fire risk components in the historic core areas of Indian cities through the AHP lens. Urban fire incidents pose significant threats to public safety and infrastructure, necessitating a systematic evaluation of key risk factors. Leveraging the AHP methodology, this research identifies and prioritizes critical criteria, including area-level vulnerabilities, socio-economic vulnerabilities, building-level capacities, emergency service capacities and historical fire incidents along with other crucial components. The hierarchical structure facilitates a nuanced analysis, allowing for comparing criteria and alternatives to derive precise priority weights.

Findings
The findings provide a strategic understanding of the factors contributing to fire risk in the historic urban cores, offering valuable insights for urban planners, emergency service personnel and policymakers. The study emphasizes strategic prioritization through the AHP framework. This study contributes to the broader discourse on urban resilience by offering a robust methodology for assessing and managing fire risks in the dynamic urban landscape of Indian cities.

Originality/value
This study contributes to the broader discourse on urban resilience by offering a robust methodology for assessing and managing fire risks in the dynamic urban landscape of Indian cities.