bluuebunny/crossref_metadata_2025_split huggingface dataset:
Navigating the influence of professional abstinence on pharmacy students: Observations and solutions from their perspective
Authors: Hall Jill J. , Tong Haley , Watson Kaitlyn E. , Tsuyuki Ross T. , Biggs Catherine , Charrois Theresa L. 
Published: January 2025

Purpose: As the scope of practice continues to evolve for pharmacists, professional abstinence is being observed by students in workplaces and practicums. Professional abstinence is defined as “consciously choosing not to provide the full scope of patient care activities”. Exposure of students to professional abstinence may cause cognitive dissonance, as they are challenged by practices that do not match what they are taught in school. This study explores pharmacy student perspectives on professional abstinence in community practice settings and their thoughts on how it could be addressed. Methods: Pharmacy students enrolled at an accredited Canadian pharmacy school with previous experience at a community practice site were invited to participate via email and social media. Data were collected between March and November 2021 through virtual individual interviews using a semistructured guide and the Zoom platform. Recordings were transcribed verbatim, and data were analyzed using cognitive dissonance theory to guide the inductive coding and interpretation of results. Results: Twenty students representing 6 pharmacy schools were recruited. Themes identified regarding students’ experiences with professional abstinence were “introspection and reflection” and “the system”, with the subthemes of emotion, excuses, professional identity and infrastructure, and inertia/disengagement, respectively. Regarding how to address professional abstinence, the themes “self as professional” and “empowering professionals” emerged. Self as professional included the subthemes of continuous learning, role modelling/mentorship, and internal drive/initiative, while empowering professionals included improved logistics, self-care, and advocacy. Conclusion: Students’ experiences with professional abstinence led to mixed feelings and influences on their professional identity. Pharmacy schools should be made aware of the phenomenon of professional abstinence and its effect on students, as they can potentially help manage learners’ cognitive dissonance and best prepare them for their future full-scope practices.
Kienböck’s Disease – Incidence and operations in Finland: a nationwide register study
Authors: Wernér Krista , Anttila Turkka , Viljakka Timo , Ryhänen Jorma , Hulkkonen Sina 
Published: January 2025

This study assessed the nationwide incidence of Kienböck’s Disease, and operations performed for Kienböck’s Disease from the Care Register for Health Care in Finland 1996–2022. The mean annual total standardized incidence rate per million person-years was 9.60 (95% CI: 9.10–10.11) and for men and women, 10.38 (95% CI: 9.63–11.13) and 8.78 (95% CI: 8.10–9.45), respectively. The incidence rate was highest for 50–59-year-olds, at 15.49. Compared with women, the incidence rate for men was higher across all age groups up to 60–69 years. Altogether, 44% of the patients were operated on, and 19% of them had multiple operations. Partial arthrodesis was the most performed procedure and salvage procedures covered 62% of all operations. Our findings provide comprehensive new evidence about Kienböck’s Disease distribution across Finland’s population and offer insights into the burden caused by operations. Level of evidence: III
Hyperconnectivity in resting-state fMRI as a marker of disease severity in Myotonic Dystrophy Type 1
Authors: Garmendia Joana , Labayru Garazi , Jiménez-Marín Antonio , Villanúa Jorge , Cortés Jesús , López de Munain Adolfo , Sistiaga Andone 
Published: January 2025

Introduction: Myotonic dystrophy type 1 (DM1) patients show both structural and functional brain alterations, including abnormal resting-state (RS) functional connectivity. Although some studies have investigated RS functional connectivity in DM1, methodological differences make it challenging to draw consistent conclusions. Objectives: This study aims to analyze 1) RS functional connectivity in DM1 patients compared to healthy controls (HC), 2) graph theory metrics, 3) longitudinal connectivity variations, and 4) the relationship between connectivity and clinical, cognitive, and structural brain data. Methodology: Twenty-one DM1 patients and 21 matched HCs underwent 3 T MRI scans, including RS fMRI. Of these, 15 DM1 patients and 13 HCs participated in the follow-up after 3 years. Additionally, DM1 patients underwent baseline clinical, molecular and cognitive assessments. Functional connectivity analysis (ROI-to-ROI) and graph theory measures were employed. Longitudinal changes in connectivity were examined, and total hyperconnectivity and hypoconnectivity values were calculated to explore correlations with clinical, brain, and cognitive correlates. Results: DM1 patients showed widespread hyperconnectivity compared to HCs. Although no statistically significant differences were found in graph theory measures, patients tended to show decreased efficiency, strength, and clustering (with moderate effect sizes). Patients remained hyperconnected over time, with a progression similar to HCs. Hyperconnectivity was associated with more severe disease, greater muscular impairment, and molecular defects, as well as lower cognitive performance. Conversely, hypoconnectivity was associated with less severe disease. Discussion: DM1 patients are characterized by brain hyperconnectivity and a less efficient brain network organization. Hyperconnectivity is discussed as a compensatory mechanism and is suggested as a disease severity marker.
Examining the Contours and Challenges of National Cybercrime Reporting Portal in Countering Digital Financial Frauds
Authors: Chandra Dakshina 
Published: January 2025

Digitalization and ease of financial transactions have spurted a ubiquitous rise in cybercrimes. In 2021, 60.8 per cent of reported cybercrime cases registered were for the motive of fraud. To tackle mounting digital financial fraud complaints, the National Cybercrime Reporting Portal was created by the Indian Cyber Crime Coordination Centre and the Ministry of Home Affairs. The portal is an integrated platform where stakeholders, such as law enforcement agencies, banks, financial intermediaries and others, are connected to prevent the outflow of money from the system, as money once out of the system becomes untraceable and non-retrievable. The portal enables requests to be sent to identified stakeholders by law enforcement authorities to block financial transactions or create a lien on the disputed money while the money is with them. The article has been written against the backdrop of Herbert Packer’s two models of the criminal process, describing two competing goals that underlie criminal legal regimes: the crime-control model and the due process model. As crime is committed in cyberspace and technology is developed to counter it, often the urgency of resolving the crime and the opacity surrounding the technology, blur the lines of permissible and impermissible actions. The article argues that this technical solution created for tackling digital financial frauds is far from perfect, and, at best, a quick fix while larger, looming questions remain unanswered. Through a series of high court cases, the article brings forth how due process requirements are often sidelined in favour of the more immediate goal of crime control in the guise of this PoliceTech, even as questions of the efficiency of the tech remain unaddressed. Nonetheless, the article recommends reforms to ensure the safe deployment of PoliceTech in India, minimize public harm and create accountability for misuse of harms arising from technology.
Disrupting of IGF2BP3-stabilized CLDN11 mRNA by TNF-α increases intestinal permeability in obesity-related severe acute pancreatitis
Authors: Lin Lihui , Lin Yansong , Guo Xianwen , Zhang Ruoyi , Ling Xin , Zhang Zewen , Lin Rong , Ding Zhen 
Published: January 2025

Abstract

Background
Obesity is a significant risk factor for severe acute pancreatitis (SAP) and is typically associated with increased intestinal permeability. Understanding the role of specific molecules can help reduce the risk of developing SAP. Claudin 11 (CLDN11), a member of the Claudin family, regulates the permeability of various internal barriers. However, the role and mechanism of CLDN11 in the intestinal permeability of obesity-related SAP remain unclear.

Methods
We evaluated intestinal permeability and the expression of CLDN11 in experimental obesity-related SAP. A recombinant adeno-associated virus carrying CLDN11 was used to treat experimental obesity-related SAP. The interaction between CLDN11 mRNA and insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) protein was predicted through bioinformatics analysis and validated by RNA immunoprecipitation and RNA pull-down assay. Additionally, tumor necrosis factor-α (TNF-α) treatment in Caco-2 cells was conducted, and the IGF2BP3/CLDN11 axis was detected. Moreover, we conducted anti-TNFα therapy and evaluated intestinal permeability and pancreatic inflammation in experimental obesity-related SAP.

Results
Downregulation of CLDN11 was observed in the intestinal epithelial cells of experimental obesity-related SAP. When the expression of CLDN11 in intestinal epithelial cells of experimental obesity-related SAP was increased exogenously, intestinal epithelial permeability and pancreatic inflammation were relieved. Overexpression of CLDN11 reduced the paracellular permeability of Caco-2 monolayer cells, while knockdown of CLDN11 increased it. IGF2BP3 bound to and regulated the stability of CLDN11 mRNA. TNF-α treatment downregulated IGF2BP3 and CLDN11 in vitro. Anti-TNFα therapy reduced intestinal permeability, alleviated pancreatitis, and improved the expression of IGF2BP3 and CLDN11 in intestinal epithelial cells in experimental obesity-related SAP.

Conclusion
CLDN11 regulates intestinal permeability in obesity-related SAP. Mechanistically, an increase in TNF-α impaired the stability of IGF2BP3-dependent CLDN11 mRNA in obesity-related SAP.
Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China
Authors: Qin Ling , Lin Fan-kang , Lv Yun-long , Tai Ze-Lin , Zhang Xu , Li Hong-lian , Li Yu , Wang Ke 
Published: January 2025

Abstract
Sorghum (Sorghum bicolor) is an important food and feed crop. Root-lesion nematodes (Pratylenchus spp.) are a group of pathogenic nematodes that cause severe economic losses in various food and cash crops. This study identified diseased sorghum plants with stunted growth and brown, rotting roots in sorghum fields in Shanxi Province, China. A species of root-lesion nematode was isolated by modified Baermann funnel method and named the GL-1 population. Afterward, the GL-1 population of root-lesion nematodes was identified as P. coffeae through a combination of morphological, rDNA-ITS and rDNA-28 S D2-D3 region techniques for molecular biological identification. We also conducted greenhouse experiments to assess the parasitism and pathogenicity of GL-1 and four other P. coffeae populations on sorghum through pot inoculation. At 60 days after inoculation, the results indicated that all five populations of P. coffeae were capable of infecting and causing damage to the sorghum plants. Sorghum is a suitable host for P. coffeae (with a reproduction factor > 1). Moreover, compared with those in the control group, the aboveground fresh weights and root fresh weights of sorghum in the five inoculation groups were significantly lower, and brown spots or even necrotic rot appeared on the roots. All five populations were highly pathogenic to sorghum, but there were significant differences in pathogenicity among the populations. This study provides a scientific basis for identifying and detecting root-lesion nematodes in sorghum.
The cell colony development is connected with the accumulation of embryogenesis-related proteins and dynamic distribution of cell wall components in in vitro cultures of Fagopyrum tataricum and Fagopyrum esculentum
Authors: Zaranek Magdalena , Pinski Artur , Skupien-Rabian Bozena , Jankowska Urszula , Godel-Jedrychowska Kamila , Sala-Cholewa Katarzyna , Nowak Katarzyna , Kurczyńska Ewa , Grzebelus Ewa , Betekhtin Alexander 
Published: January 2025

Abstract

Background
Due to the totipotency of plant cells, which allows them to reprogram from a differentiated to a dedifferentiated state, plants exhibit a remarkable regenerative capacity, including under in vitro culture conditions. When exposed to plant hormones, primarily auxins and cytokinins, explant cells cultured in vitro can undergo differentiation through callus formation. Protoplast culture serves as a valuable research model for studying these processes in detail. This knowledge is particularly relevant for improving common and Tartary buckwheat species. To gain deeper insights into the stages of cell development from protoplasts—such as cell division, cell colony formation, and microcalli development—we focused on analyzing proteomes, cell wall composition, and changes in the expression profiles of selected genes in Fagopyrum protoplast cultures.

Results
The results demonstrate a significant accumulation of somatic embryogenesis-related proteins like late embryogenesis abundant proteins (embryogenic protein-DC-8-like, seed biotin-containing protein) and endochitinases during the developmental path of protoplast-derived cultures. Additionally, we noted an extensive increase in seed storage proteins like vicilin, oleosins, and seed biotin-containing proteins during the culture. Investigation of somatic embryogenesis-associated transcription factors revealed massive up-regulation of LEAFY COTYLEDON1 for the 50th day of F. tataricum protoplast-derived cultures. However, for BABY BOOM, the transcription factor was noted to be down-regulated during the development of cell colonies. Furthermore, we demonstrated the variable distribution of cell wall components like pectin side chains, arabinogalactan proteins (AGPs) and extensins (EXTs), indicating the reorganisation of cell wall composition during the culture period.

Conclusions
This study revealed changes correlating with regaining embryogenic competence during the development of Fagopyrum protoplast-derived cell colonies. Our findings revealed variable expression levels of genes and proteins associated with somatic embryogenesis. This analysis identified an increase in seed storage proteins that play a significant role in the somatic somatic embryogenesis pathway of regeneration. Furthermore, the relationship between transcription factors and these processes seems to be connected with regaining somatic cells’ totipotency and promoting embryogenic competence of protoplast-derived cell colonies. Additionally, we observed dynamic changes in cell wall composition during the development of the protoplast-derived cultures.

Clinical trial number
Not applicable.
Uncoded chronic kidney disease prevalence in secondary care: a retrospective audit with population health implications
Authors: Dolan Samantha , Anand Ajitesh , Kalra Philip A. , Stewart Stuart 
Published: January 2025

Abstract

Background
One million patients are estimated to have undiagnosed chronic kidney disease (CKD) in England. Clinical coding in CKD is associated with improved management and lower acute kidney injury (AKI), unscheduled care and mortality risk. Primary care’s role in coding CKD is well documented. However, there is scant evidence on CKD coding quality in secondary care. Primary aims: to measure total and coded/uncoded CKD prevalence on admission and discharge, and conversion of uncoded to coded CKD in secondary care. Secondary aims: to map coding status to kidney health inequality themes and to measure predictors of coding, death and AKI.

Methods
Retrospective audit in an acute medical hospital ward in England, April 2022-February 2023. Descriptive statistics include counts/percentages for categorical data, prevalence estimates and rates. Logistic regression measured significant predictors (p = < 0.05) of receiving a diagnostic CKD code on discharge, risk of death, and of AKI.

Results
Uncoded CKD prevalence using discharge estimated GFR (eGFR) was 58.7% (n = 283), equating to 1.1 cases uncoded CKD per bed/month and 13.7 cases uncoded CKD per bed/year. Conversion of uncoded to coded CKD at discharge was only 6.7%. Hypertension and advanced CKD were significant predictors of coding CKD on discharge in uncoded patients. Age, sex, indices of multiple deprivation, and AKI were significant predictors of death during admission. Advanced CKD was a significant predictor of AKI during admission.

Conclusions
Uncoded CKD is highly prevalent in an acute medical hospital ward highlighting opportunity to improve coding in another part of the health system in addition primary care.
Socioeconomic disparities in child malnutrition: trends, determinants, and policy implications from the Kenya demographic and health survey (2014 - 2022)
Authors: Okutse Amos O. , Athiany Henry 
Published: January 2025

Abstract

Background
Child malnutrition remains a critical public health problem, with socioeconomic factors playing a significant role. Socioeconomic factors include household income, parental education, and access to healthcare, which influence a child’s nutritional status. Despite overall progress in reducing under-five child malnutrition in Kenya, disparities persist. This paper analyzes changes, and determinants of child malnutrition, contributions of these determinants to health inequality, and their potential use in the screening for chronic malnutrition in children under five years.

Methods
We use data from the Kenyan Demographic and Health Survey (KDHS 2014 and 2022) and analyze malnutrition using three indicators: Stunting, underweight, and wasting. The determinants of malnutrition are analyzed using multivariate logistic regression. Trends in socioeconomic inequality are analyzed using concentration indices and visualized using concentration curves. Wagstaff decomposition is used to explore the contributions of determinants to inequality in child malnutrition. We investigate diagnostic utility using sensitivity, specificity, predictive values, and area under the ROC curve.

Results
Socioeconomic inequality in under-five child malnutrition increased between 2014 and 2022, with children from the poorest socioeconomic quintiles being disproportionately affected. A child’s age (in months) (Adjusted Odds Ratio [AOR] = 1.01; 95% Confidence Interval [CI]: 1.01 – 1.02), being born to a household in the poorest socioeconomic quintile (AOR = 2.67; 95%CI: 1.92 - 3.72), and sex (male) (AOR = 1.50; 95%CI: 1.35 – 1.67) were associated with an increased risk of stunting. The mother’s age, sex of the child (male), and household socioeconomic status (poorest) was associated with an increased risk of being underweight and wasted, whereas residence was associated with an increased risk of wasting alone after adjusting for potential confounders. A household’s socioeconomic status was the largest contributing factor to health inequality. Sensitivity, specificity, and AUC values were 67.4% (95% CI: 66.4% – 68.4%), 50.6% (95%CI: 50.0% - 51.1%), and 0.59 (95%CI: 0.58 – 0.60), respectively, when using socioeconomic status as a screening tool for stunting.

Conclusion
Socioeconomic disparities are a major barrier to reducing child malnutrition in Kenya, with children from lower socioeconomic quintiles at a greater risk of stunting, underweight, or wasting. This study identifies a child’s sex, age, and household socioeconomic status as key predictors of malnutrition, highlighting the need to include these factors in public health interventions. Addressing these disparities with targeted strategies considering immediate health risks and underlying socioeconomic challenges is essential for equitably improving child health outcomes.
A longitudinal blended learning curriculum for bedside ultrasound education in pulmonary and critical care fellowship
Authors: Kuperstein Harry , Gada Kunal , Alam Werda , Ahmad Sahar 
Published: January 2025

Abstract

Background
There exists no standardized longitudinal curriculum for teaching bedside ultrasonography (US) in Pulmonary and Critical Care Medicine (PCCM) fellowship programs. Given the importance of mastering bedside US in clinical practice, we developed an integrated year-long US curriculum for first-year PCCM fellows.

Methods
11 first-year PCCM fellows completed the entire seven-step Blended Learning Curriculum. We provide results from an evaluation of Step I, the initial training course. Evaluation included a 17-question multiple-choice knowledge test and a hands-on skill exam delivered pre-, immediately post-, and 6 months post-course. Performance on these same evaluation measures was compared between learners who completed a traditionally designed curriculum, which contained a formal in-person didactic course, and learners who completed a blended learning curriculum covering the same learning objectives.

Results
All learners showed a significant improvement immediately after the course in both knowledge (p = 0.007) and skills (p = 0.004) with adequate retention of both knowledge and skills after 6 months. Scores on a multiple-choice knowledge test increased from a median (interquartile range [IQR]) of 24% (15–41%) pre-course to a median of 71% (59–82%) post-course, while scores on a hands-on skill exam increased from a median of 16% (7–45%) pre-course to a median of 87% (74–94%) post-course. There was no difference in learning or retention between those who learned via the blended learning model as compared with a more traditional model. Learners agreed the course was well-designed, with relevant learning topics, sufficient time to learn, and fair evaluation modalities. The blended learning model required 15 fewer faculty-hours than the traditional learning model.

Discussion
A blended learning model for bedside US education implemented at a single PCCM fellowship performs comparably to a traditional model for both acquisition and retention of knowledge and skills. The incorporation of asynchronous learning mitigates the barrier of insufficient time and quantity of US skilled teaching faculty that many PCCM fellowships face and provides flexibility to both instructors and learners.