bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of non-small cell lung cancer (NSCLC) proliferation through targeting G6PD
Authors: Chanda Makamas , Anuntasomboon Pornchai , Ruangritchankul Komkrit , Cheepsunthorn Poonlarp , Cheepsunthorn Chalisa L. 
Published: December 2023

Background
Mounting evidence has linked cancer metabolic reprogramming with altered redox homeostasis. The pentose phosphate pathway (PPP) is one of the key metabolism-related pathways that has been enhanced to promote cancer growth. The glucose 6-phosphate dehydrogenase (G6PD) of this pathway generates reduced nicotinamide adenine dinucleotide phosphate (NADPH), which is essential for controlling cellular redox homeostasis.

Objective
This research aimed to investigate the growth-promoting effects of G6PD in non-small cell lung cancer (NSCLC).

Methods
Clinical characteristics and G6PD expression levels in lung tissues of 64 patients diagnosed with lung cancer at the King Chulalongkorn Memorial Hospital (Bangkok, Thailand) during 2009-2014 were analyzed. G6PD activity in NSCLC cell lines, including NCI-H1975 and NCI-H292, was experimentally inhibited using DHEA and siG6PD to study cancer cell proliferation and migration.

Results
The positive expression of G6PD in NSCLC tissues was detected by immunohistochemical staining and was found to be associated with squamous cells. G6PD expression levels and activity also coincided with the proliferation rate of NSCLC cell lines. Suppression of G6PD-induced apoptosis in NSCLC cell lines by increasing Bax/Bcl-2 ratio expression. The addition of D-(-)-ribose, which is an end-product of the PPP, increased the survival of G6PD-deficient NSCLC cell lines.

Conclusion
Collectively, these findings demonstrated that G6PD might play an important role in the carcinogenesis of NSCLC. Inhibition of G6PD might provide a therapeutic strategy for the treatment of NSCLC.
Restudy of shoulder motion in the theropod dinosaur Mononykus olecranus (Alvarezsauridae)
Authors: Senter Philip J. 
Published: December 2023

Background
Range of motion in the forelimb of the Upper Cretaceous theropod dinosaur Mononykus olecranus, a member of the family Alvarezsauridae, has previously been investigated. However, the method used to investigate range of motion at the shoulder in M. olecranus did not follow the standardized procedure used in subsequent studies. The latter procedure yields more reliable results, and its standardization provides that its results are directly comparable to the results of similar studies in other species. I therefore reinvestigated the range of motion at the shoulder in M. olecranus, using the latter procedure.

Methods
Casts of the left scapula and coracoid of M. olecranus were posed on a horizontal surface, supported from beneath with modeling clay, with the medial surface of the scapula facing toward the horizontal surface. A cast of the left humerus was posed at the limits of motion through the transverse and parasagittal planes. Photos of the poses in orthal views were superimposed and used to measure range of motion, which was measured as the angle between lines drawn down the long axis of the humerus in each position.

Results
Through the transverse plane, the humerus of M. olecranus could be elevated to a subhorizontal position and depressed to a subvertical position. It could move through the parasagittal plane from a subvertical position at full protraction to a position above the horizontal at full retraction. These results correct the previous mischaracterization of shoulder motion in M. olecranus as restricted to a small arc with the arms held in a permanent sprawl. The range of humeral motion in M. olecranus is much greater than that found by the previous method and allowed the animal to tuck its arms in at the sides, in addition to allowing them to sprawl so as to orient the palm downward. The wide range of humeral motion allowed M. olecranus to forage for insects by employing hook-and-pull digging at surfaces with a wider range of orientations than the previous study showed to be possible.
Origin and In-Office Treatment of Retrograde Cricopharyngeus Dysfunction
Authors: Mailly Marie , Baudouin Robin , Thibault Claire , Hans Stephane , Lechien Jerome R. 
Published: January 2025

ImportanceRetrograde cricopharyngeus dysfunction (R-CPD) is an emerging disorder associated with disabling symptoms. The origin of R-CPD remains unknown.ObjectiveTo investigate the development of symptoms, diagnosis approach, and therapeutic outcomes of R-CPD in patients treated with in-office botulinum toxin injection (BTI) into the cricopharyngeus.Design, Setting, and ParticipantsThis was a case series including patients with R-CPD who were consecutively and prospectively recruited from April 2022 to May 2024 in an academic hospital. Semistructured interviews were conducted to collect and analyze data on each patient’s clinical history, potential causes or factors associated with R-CPD development, diagnostic approaches, and symptom presentation.InterventionClinic-based (in-office) BTI into the cricopharyngeus.Outcomes and MeasuresAssociations with laryngopharyngeal reflux disease, patients’ Reflux Symptom Score-12 (RSS-12), and BTI effectiveness, revisions, and complications were evaluated.ResultsThe case series comprised 106 patients with R-CPD treated with BTI (52 females [49.1%] and 54 males [51.9%]). Their mean (SD) age at symptom onset was 13.6 (7.7) years, and at diagnosis, 30.4 (6.4) years. Sixty-eight patients (64.2%) had potential congenital R-CPD, according to the parents’ testimonies. A family history was reported in 18 of 62 cases (29.0%). In 105 cases (99.1%), patients made the diagnosis themselves despite medical consultations (n = 162), empirical treatments (n = 113), and additional examinations (n = 92). The cumulative success rate of BTI was 90.6% (96 of 106 patients). In 26 cases (24.5%), additional injections were administered to address the symptoms. Family history of R-CPD was a negative predictor of single-BTI success. Dysphagia was the primary adverse effect occurring after 89 of 126 BTIs (70.6%) and lasted a mean (SD) of 16.3 (12.0) days. In 10 cases, operating-room BTI was administered after primary in-office BTI.Conclusions and RelevanceR-CPD is an emerging and poorly known disorder associated with high rates of ineffective consultations, additional examinations, and self-diagnosis by patients. In-office BTI was associated with a high rate of partial or total symptom relief and long-term effectiveness.
Enhancing Immunomodulation and Osseointegration of Bone Implants via Thrombin‐Activated Platelet‐Rich Plasma Self‐Assembly
Authors: Shi Xiaotong , Wang Zongliang , Bi Zhiguo , Liu Jianguo , Zhang Peibiao 
Published: January 2025

AbstractPlatelet‐rich plasma (PRP) is characterized by elevated concentrations of growth factors that facilitate bone repair. Nonetheless, the effective integration of PRP with bone implants and the sustained release of its active constituents pose significant challenges. In this study, thrombin is grafted onto the surface of polyetheretherketone (PEEK) via an N,N'‐Disuccinimidyl Carbonate (DSC) linker and the retained enzymatic activity of thrombin enables the controlled activation of PRP self‐assembly, resulting in the formation of a functional bio‐gel layer. The optimal thrombin concentration to be 100 U/ mL−1 is determined, at which point both the grafting amount and enzymatic activity of thrombin reaches their peak, with no further increases observed at higher concentrations. PRP solutions with varying platelet enrichment ratios are subsequently activated on the thrombin‐grafted PEEK surface, yielding self‐assembled bio‐gels capable of sustained growth factor release for up to 16 days. The thrombin‐activated PRP bio‐gel on PEEK surface not only enhances in vitro cell adhesion, proliferation, osteogenic differentiation, vascularization and specific polarization of macrophages, but also effectively facilitates in vivo angiogenesis, immunomodulation and bone formation in a platelet dose‐dependent manner. Consequently, the thrombin‐activated PRP gel presents a promising strategy for the biological functionalization of PEEK implants in orthopedic applications.
Mesenchymal Stem Cells With Polydopamine‐Coated NaGdF4 Nanoparticles with Ca2+ Chelation Ability for Ischemic Stroke Therapy
Authors: Wu Shiman , Chu Xu , Lv Guanglei , Gao Jiahao , Huang Yuxin , Li Huiyan , Jiang Xingwu , Liu Yanyan , Zhang Jiawen , Fang Xiangming , Yao Zhenwei , Bu Wenbo 
Published: January 2025

AbstractMesenchymal stem cells (MSCs) transplantation is a promising therapeutic strategy for ischemic stroke. However, the survival of transplanted MSCs is often compromised by the excessive levels of reactive oxygen species (ROS) and calcium ions (Ca2+) in the ischemic microenvironment following blood flow occlusion. In this study, a protective strategy is developed using functional nanomaterials to escort and shield MSCs. Specifically, NaGdF4@PDA‐ALD nanoparticles (NPANs) are synthesized, featuring a NaGdF4 core coated with polydopamine (PDA) for ROS scavenging and further modified with alendronate sodium (ALD) for Ca2+ chelation. The internalization of NPANs by MSCs protected them from oxidative damage and calcium overload, thereby promoting their viability and functionality. Furthermore, NaGdF4 generated T1 signal enhancement, enabling in vivo tracking of MSCs via magnetic resonance imaging. The NPANs‐treated MSCs demonstrated improved survival and migration to the ischemic region, promoting blood flow restoration and angiogenesis. These findings confirm the feasibility of employing functional nanoparticles to augment MSCs‐based therapies, offering a promising strategy to improve their therapeutic efficacy in ischemic stroke treatment.
Vertical Memristive Crossbar Array for Multilayer Graph Embedding and Analysis
Authors: Han Janguk , Jang Yoon Ho , Moon Ji Won , Shim Sung Keun , Cheong Sunwoo , Lee Soo Hyung , Park Tae Won , Han Joon‐Kyu , Hwang Cheol Seong 
Published: January 2025

AbstractGraph data structures effectively represent objects and their relationships, enabling the modeling of complex connections in various fields. Recent work demonstrate that metal at diagonal crossbar arrays (m‐CBA) can effectively represent planar graphs. However, they are unsuitable for representing multilayer graphs having multiple relationships across different layers. Using conventional software, embedding multilayer graphs in high‐dimensional Euclidean spaces introduces significant mathematical complexity and computational burden, often resulting in information loss. This study proposes a unique graph embedding (mapping) method utilizing a fabricated vertical m‐CBA (vm‐CBA), where a custom‐built measurement system thoroughly validated its functionality. This structure directly maps multilayer graphs into a 3D vm‐CBA, accurately representing inter‐layer and intra‐layer connections. The practical link prediction and information scores across various real‐world datasets demonstrated that vm‐CBA achieved enhanced accuracy compared to conventional embeddings, even with a significantly decreased number of operations.
Phenanthrenequinone‐Modified Conjugated Polymer Enabling Photocatalytic H2O2 Generation via Efficient O2− Conversion
Authors: Sun Xinyu , Yang Tingyu , Dong Yuming , Ji Rong , Zhao Hui , Zhang Jiawei , Zhu Yongfa 
Published: January 2025

AbstractThe photocatalytic O2 reduction reaction offers a promising approach to synthesizing H2O2. Nevertheless, the low conversion efficiency of O2− constrains the efficiency of photocatalytic H2O2 production. This is because O2 can readily obtain electrons to generate O2−, but it is challenging to reacquire electrons after generating O2−. Accordingly, this study proposes the improvement of the conversion efficiency of O2− through the directional enrichment of photogenerated charges at sites of O2− reduction and stabilization of O2−. This study introduces a phenanthrenequinone group with adjacent carbonyl groups as the O2− reduction reaction site. The directional enrichment of photogenerated charges at the O2− reduction site enhances the probability of O2− obtaining electrons. Concurrently, the special adsorption configuration enhances the adsorption, and stabilizes·O2− on the catalyst surface, accelerating the conversion of O2− to H2O2 and achieving an H2O2 generation rate of 3400 µmol g−1 h−1. This work presents an innovative tactic regarding the efficient reduction of O2− to H2O2, which informs the structural design of advanced photocatalytic systems for the production of H2O2.
Functional Analyses of SATB2 Variants Reveal Pathogenicity Mechanisms Linked With SATB2‐Associated Syndrome
Authors: Ukita Nao , Ogawa Takuya , Yamada Mamiko , Takeuchi Chisen , Kosaki Kenjiro , Moriyama Keiji 
Published: January 2025

ABSTRACTSATB2‐associated syndrome (SAS) is characterized by intellectual disability, neurodevelopmental disorders, cleft palate, and dental abnormalities. SAS is caused by variants in the special AT‐rich sequence‐binding protein 2 (SATB2), which encodes a transcription factor containing two CUT domains and a homeobox (HOX) domain. Here, we report the case of a 16‐year‐old male diagnosed with SAS using exome sequencing and investigate the functional consequences of previously reported SATB2 variants, including those in this case. The patient carried a heterozygous missense variant (c.1147G>C, p.A383P) in SATB2, which was predicted to be pathogenic in silico but was absent from public databases. Immunofluorescence assays demonstrated that SATB2 proteins with variants in the CUT2 domain predominantly localized to the cytoplasm. Functional analysis further revealed that wild‐type SATB2 increased the activity of the Msx1 promoter, which is involved in palatogenesis and tooth development, whereas variants in the CUT1 domain disrupted this transcriptional activation. These findings suggest that the nuclear localization signal of SATB2 resides in the CUT2 domain and that Msx1 promoter impairment owing to SATB2 variants may contribute to the pathogenesis of cleft palate and tooth agenesis in SAS. This research highlights a novel pathogenic variant and the functional implications for understanding SAS.
Supporting ‘Doing Development Differently’ Effectively: Analysing Attributes, Networks and Impacts of Donor‐Supported Pacific Development Coalitions
Authors: Craney Aidan , Chamberlain Dan , Roche Chris 
Published: January 2025

ABSTRACTDespite growing appreciation for the complexity of development and the need for adaptability, there remains a limited evidence base of how, where and why adaptive approaches to development improve development outcomes. This paper examines the organisational and political attributes of support provided to locally led development initiatives in the Pacific region supported by an Australian government development programme, as well as the extent to which these attributes were present in initiatives deemed by programme staff to be more or less successful. Findings include that positive outcomes are more likely to emerge from partners led by women working in politically stable environments. Rather than endorsing donors to support only such projects, we share a combination of findings to offer insights into how the impacts of development initiatives working in politically tricky settings may be understood holistically and with nuance and flexibility, leading to better project design and evaluation.
An Inertia Correction Scheme for Hydrodynamic Lubrication Problems
Authors: Ozen Seyhan , Azeloglu C. Oktay 
Published: January 2025

ABSTRACTA new simplified numerical approach for accurately calculating the bearing pressure distribution in one‐dimensional hydrodynamic lubrication problems, particularly including convective fluid inertia and film discontinuities, is presented. The method proposes a simple inertia correction scheme using a non‐uniform finite difference method based on the Reynolds equation. Two possible approaches to estimating the pressure correction due to fluid inertia are discussed: the Bernoulli effect and the averaged inertia. The results obtained for various operating conditions, especially by employing the average fluid inertia method, are found to be almost identical to the full Navier–Stokes (CFD) results and are more generalized. The proposed method may provide extremely fast calculation with accuracy.
Anatomy of the Critically‐Endangered Anji Salamander (Hynobius amjiensis) Provides New Insights Into Morphological Evolution of Salamanders
Authors: Chen Cang‐Song , Jia Jia , Wang Xian‐Ting , Yang Jia , Gao Ke‐Qin 
Published: January 2025

ABSTRACTThe Anji Salamander (Hynobius amjiensis) is a critically‐endangered amphibian endemic to the Tianmushan Mountain area in southeastern China. As most of its congeneric species in the ancestral salamander family Hynobiidae, the osteology of H. amjiensis has remained essentially unknown and has hampered efforts in understanding morphological evolutionary patterns of early salamanders. Here, we investigate the skeletal anatomy of H. amjiensis based on microcomputed tomography scans of post‐metamorphosed juvenile and adult specimens. Our results reveal Hynobiidae has more early‐tetrapod‐like plesiomorphic characters than expected, as H. amjiensis has a stapedial foramen in the middle ear and two centralia and a centrale‐radius contact in the limb. We demonstrate that Hynobius amjiensis is the first known living salamander species with a stapedial foramen whose absence was believed to unite salamanders and anurans, and hence opens major questions on the evolution of the middle ear in modern amphibians: if some salamanders and caecilians had a stapedial foramen inherited from their common ancestor, when and how many times was the foramen lost independently in modern amphibians, and how did this structural loss impact the phylogenetic evolution of salamander clades? Our findings of hyper‐ossified pectoral and pelvic girdles and loss of postminimus in the pes in H. amjiensis demonstrate that functional morphological features in hynobiids are potentially informative in phylogeny and ontogeny of early salamanders.
Reliable Methodology for Measuring SERS Enhancement Factor on Colloidal and Solid Substrates: A Practical Guide
Authors: Mercedi Anna , Cardoni Francesco , Toffanello Francesca , Reguera Javier , Meneghetti Moreno , Litti Lucio 
Published: January 2025

ABSTRACTThis study presents a robust and practical methodology for quantifying the Surface‐Enhanced Raman Spectroscopy (SERS) Enhancement Factor (EF) on both colloidal and solid substrates. SERS, a powerful technique for enhancing the Raman signals of analytes near plasmonic surfaces, has found extensive applications in fields such as biomedicine and materials science. However, inconsistencies in EF measurement methods have hindered cross‐comparisons between different SERS substrates. This work addresses these challenges by providing a standardized protocol for EF determination for a wide variety of substrates composed of gold and silver nanoparticles, as well as nanostructured surfaces. Using p‐mercaptobenzoic acid (p‐MBA) as a model non‐resonant probe, the approach accounts for key variables including substrate geometry, sampling volume, and substrate inhomogeneity. This reliable and reproducible method offers a practical framework for researchers to assess the performance of SERS substrates with simple instruments and techniques that should be easily available in a Raman spectroscopy laboratory.
A Bibliometric Review of Digitalization in Tourism Supply Chains in the Context of Industry 4.0
Authors: Luu Nghia Thi Minh , Pham Trang Huong , Siriwardana Anushka , Nguyen Lam Canh , Tran Duy Le Anh 
Published: January 2025

ABSTRACTThe tourism industry has experienced significant turbulence over the past two decades, characterized by dramatic advancements and formidable challenges. One notable development has been the application of Industry 4.0 technologies to various areas of the tourism supply chain. This review employs the bibliometric method to identify the research evolution of technologies in the tourism supply chain domains. The findings based on research clusters and streams indicate that, before Industry 4.0, literature predominantly focused on fundamental technologies such as IT, ICT, Internet, and RFID. After its introduction in 2011, research began incorporating some forms of advanced technologies, albeit in limited ways. However, following the outbreak of COVID‐19, the application of Industry 4.0 technologies has become more widespread, emerging as a primary research theme. Throughout the period, sustainability has been a consistent research interest, with the belief that technological applications could enhance business performance and customer experience, and mitigate social and environmental impacts. A discussion of the future research directions in the surveyed domain follows the research trend analysis.
Association Analysis of Rare CNTN5 Variants With Autism Spectrum Disorder in a Japanese Population
Authors: Hadi Abdul Fuad , Arta Reza K. , Kushima Itaru , Egawa Jun , Watanabe Yuichiro , Ozaki Norio , Someya Toshiyuki 
Published: January 2025

ABSTRACTBackgroundContactin‐5 (CNTN5), a neural adhesion molecule involved in synaptogenesis and synaptic maturation in the auditory pathway, has been associated with the pathophysiology of autism spectrum disorder (ASD), particularly hyperacusis. To investigate the role of rare CNTN5 variants in ASD susceptibility, we performed resequencing and association analysis in a Japanese population.MethodsWe resequenced the CNTN5 coding regions in 302 patients with ASD and prioritized rare putatively damaging variants. The prioritized variants were then genotyped in 313 patients with ASD and 1065 controls. Subsequently, we conducted an association study of selected variants with ASD in 614 patients with ASD and 61 057 controls. Clinical data were reviewed for patients carrying prioritized variants.ResultsThrough resequencing, we prioritized three rare putatively damaging missense variants (W69G, I227L, and L1000S) in patients with ASD. Although we found a nominally significant association between the I227L variant and ASD, it did not remain significant after post hoc correction. Hyperacusis was found in three out of nine patients carrying prioritized variants.ConclusionThis study does not provide evidence for the contribution of rare CNTN5 variants to the genetic etiology of ASD in the Japanese population.