bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlations between primary tumour location, biomarkers of inflammation and lung injury, and postoperative pulmonary complications in patients underwent laparoscopic colorectomy: a propensity score matched analysis of 300 patients
Authors: Li Hui-xian , Che Li , Li Yuan , Wang Tai-hang , Min Fang-di , Xu Lei , Wang Meng , Zheng Zhao-xu , Qu Shi-ning , Wang Fei , Tang Wei , Wei Shi-jing , Sun Yu-lin , Zheng Hui , Yan Tao 
Published: January 2025

IntroductionThe impact of distinct primary colorectal cancer (CRC) sites on lung injury and complications remains largely unexplored, despite the palpable differences in surgical positions, procedures, and the resulting mechanically induced respiratory pressures at each site.Materials and methodsThis study employed a forwards-looking approach utilising the propensity score matching (PSM) method; 300 patients with pathological CRC after laparoscopic surgery from April 2019 to May 2023 were enrolled. Two categories were bifurcated based on their surgical locations: the rectosigmoid colon (RSC) group and the descending/ascending colon (DAC) group, with a 2:1 ratio. The occurrence of postoperative pulmonary complications (PPCs) within a 30-day postoperative period was meticulously evaluated. Additionally, assessments have been performed for plasma biomarkers of immune response dynamics and lung injury (plasma soluble advanced glycation end-product receptor [sRAGE], angiopoietin-2 [ANG-2], interleukin-1β/6 [IL-1β/IL-6]) and other parameters.ResultsAlthough the increase in postoperative lung epithelial damage, as indicated by the plasma sRAGE levels, was significant in the RSC group (DAC vs. RSC; 1029.6 [576.8–1365.2] vs. 1271.6 [896.3–1587.6]; odds ratio=0.999; 95% CI: 0.998 to 1.000; P=0.007), a significantly increased percentage of PPCs was observed in the DAC group (DAC vs. RSC; hazard ratio=1.669; 95% CI, 1.141 to 2.439; P=0.008). A univariate Cox proportional hazards model revealed that sRAGE, ANG-2, IL-1β, and IL-6 levels were not correlated with the incidence of time-to-PPCs across the two cohorts (P&gt;0.05). Propensity score-weighted Cox regression and causal mediation analysis further demonstrated that the DAC site directly affected the incidence of PPCs, regardless of the other baseline confounders and clinical covariates related to the tumour site and PPCs.ConclusionThe primary site of CRC is an independent predictor of the development of PPCs. Despite the steep Trendelenburg position of the RSC group inciting more pulmonary stress, inflammation and lung epithelial injury, as indicated by higher sRAGE, it demonstrated a lower PPCs occurrence relative to its DAC counterpart, with a slightly inclined or reversed Trendelenburg position. None of the plasma biomarkers of inflammation or lung injury indicated sufficient prognostic value for PPCs.
Growth differentiation factor-15 as a biomarker for intensive care unit-acquired weakness: a meta-analysis
Authors: Wang Bing-Han , Qi Meng-Ying , Yang Zheng , He Gui-Lan , Meng Si-Ya 
Published: January 2025

BackgroundGrowth differentiation factor-15 (GDF-15) may be a potential biomarker for intensive care unit-acquired weakness (ICU-AW). In this study, we aimed to quantitative analysis the levels of GDF-15 in patients with ICU-AW and in non-ICU-AW, and then to determine its potential diagnostic utility.MethodsTwo researchers separately conducted a systematic search of the relevant studies up to May 2023 in various literature databases (PubMed, Cochrane, Web of Science, Embase, and CINAHL). Studies were selected according to the inclusion and exclusion criteria, and quality evaluation of the included studies was conducted by using QUADAS-2 provided by Review Manager 5.3. The software packages Meta Disc (C1.4) and Stata17.0 were used for the meta-analysis. The data were combined with fixed-effects model, and the summary receiver operating characteristic curve was drawn to evaluate the overall diagnostic accuracy of GDF-15.ResultsWe identified 6 eligible studies comprising 401 patients with ICU-AW. The sensitivity, specificity, diagnostic odds ratio (DOR), and area under the curve (AUC) for the discriminative performance of GDF-15 as a diagnostic biomarker were 0.82 (95% confidence interval (CI):0.78–0.86), 0.83 (95% CI: 0.61–0.88), 21.39 (95% CI: 13.36–34.24), and 0.88 (95% CI: 0.85–0.91), respectively.ConclusionGDF-15 is a candidate biomarker in diagnosing of ICU-AW from non-ICU-AW.
Genome-wide identification of short-chain dehydrogenases/reductases genes and functional characterization of ApSDR53C2 in melanin biosynthesis in Arthrinium phaeospermum
Authors: Liao Jiao , Wang Yisi , Liu Han , Liu Sijia , Yan Peng , Chen Hang , Li Shujiang 
Published: January 2025

IntroductionArthrinium phaeospermum can cause large areas wilted and death of Bambusa pervariabilis × Dendrocalamopsis grandis, resulting in serious ecological and economic losses. Previous studies found that the appressorium of A. phaeospermum must form to invade the host cells and cause disease. A short-chain dehydrogenase/reductase gene has been shown to maintain the osmotic pressure of the appressorium by synthesizing fungal melanin to penetrate the plant epidermis and cause disease. The SDR gene family of A. phaeospermum was found to be highly expressed during the penetration in the transcriptome sequencing results. Still, the relationship with melanin biosynthesis of A. phaeospermum is not clear.MethodsWe aimed to predict the biological function of the SDR gene family in A. phaeospermum, identify key ApSDR genes with pathogenic roles, and explore the pathogenic mechanism. We have characterized the SDR family of A. pheospermum bioinformatically. Candidate ApSDRs screened by transcriptome sequencing were compared by qPCR experiments to obtain key ApSDRs that may play an important role in infestation and adversity resistance. Knockout mutants, the co-knockout mutant, and backfill mutants of key ApSDRs were obtained for phenotypic and stress conditions analysis. We explored and validated the pathogenic mechanisms through cellulose membrane penetration experiments and analysis of melanin-related gene synthesis levels.Results and discussion180 ApSDRs were identified bioinformatically. After screening six candidate ApSDRs with noticeably elevated expression using transcriptome sequencing, qPCR experiments revealed that ApSDR53C2 and ApSDR548U2 had the highest expression. The results of phenotypic and stress conditions analysis indicate that ApSDRs are critical for the growth, development, stress response, and fungicide resistance of A. phaeospermum. The pathogenicity analysis revealed that ApSDR53C2 and ApSDR548U2 play important roles in virulence, with ApSDR53C2 having a stronger effect. A comparison of melanin synthesis levels between wild-type and ΔApSDR53C2 strains showed that ApSDR53C2 positively regulates melanin biosynthesis to promote penetration. The findings demonstrate that ApSDRs are essential for A. phaeospermum to withstand stress and facilitate melanin biosynthesis, which in turn contributes to its virulence.
Improving cognitive impairment through chronic consumption of natural compounds/extracts: a systematic review and meta-analysis of randomized controlled trials
Authors: Hoang Long Ngo , Lee Haesung , Lee Sook Jeong 
Published: January 2025

IntroductionThis systematic review and meta-analysis aimed to compare the efficacy of extended supplementation (≥6 weeks) with natural compounds or extracts in improving cognitive function in patients with mild cognitive impairment (MCI) or Alzheimer’s disease (AD).MethodsA comprehensive literature search was conducted across Cochrane, PubMed, PsycARTICLES, Scopus, and Web of Science databases from inception to April 10, 2024. Eligible studies were randomized controlled trials evaluating cognitive outcomes in patients with MCI or AD using the Mini-Mental State Examination (MMSE) and the Alzheimer’s Disease Assessment Scale-Cognitive Subscale (ADAS-Cog).ResultsFrom an initial pool of 6,687 articles, 45 were deemed relevant for qualitative analysis. Of these, 37 studies demonstrated improvements or positive trends in cognitive outcomes with natural compound or extract supplementation. A total of 35 studies met the criteria for meta-analysis. The meta-analysis, involving 4,974 participants, revealed significant improvements in ADAS-Cog scores (pooled standardized mean difference = −2.88, 95% confidence interval [CI]: −4.26 to −1.50; t24 = −4.31, p &lt; 0.01) following supplementation. Additionally, a suggestive trend toward improvement in MMSE scores was observed in a subgroup analysis of 1,717 participants (pooled standardized mean difference = 0.76, 95% CI: 0.06 to 1.46, t18 = 2.27, p = 0.04).ConclusionThese findings support the potential cognitive benefits of extended (≥6 weeks) supplementation with natural compounds or extracts in individuals with MCI or AD. Further research is warranted to confirm these results and elucidate the underlying mechanisms.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/.
Temporal and geographical dynamics of early-onset Parkinson’s disease burden: insights from the Global Burden of Disease Study 2021
Authors: Li Yong , Tan Dianhui , Luo Cheng , Chen Junchen 
Published: January 2025

IntroductionEarly-onset Parkinson’s disease (EOPD) is a rare degenerative condition of the nervous system that primarily affects individuals of working age. Its distinct clinical and genetic features make it a critical area of study in neurological research and public health.Methods and materialsThis analysis utilized data from the Global Burden of Disease Study 2021, covering 371 diseases and injuries across 204 countries and territories from 1990 to 2021. The study focused on individuals aged 15–49 to characterize EOPD. Data on occurrence, frequency, mortality, and disability-adjusted life years (DALYs) were collected. Sociodemographic measures were used to analyze patterns and differences. Statistical methods, including joinpoint regression and decomposition analysis, were employed to identify temporal patterns and factors influencing variations in EOPD burden over time.ResultsThe global incidence of EOPD saw a significant increase between 2000 and 2009, with an average annual percentage change (AAPC) of 4.3%, continuing at a slower rate (AAPC 1.6%) from 2010 to 2021. By 2021, the incidence reached 2.1 cases per 100,000 population, up from 1.0 in 1990. Males had a higher incidence (AAPC 2.3%) compared to females (AAPC 0.8%). In 2021, 59.7% of the 81,047 global EOPD cases were male. Significant increases were observed in East Asia (AAPC 5.1%), Andean Latin America (AAPC 2.0%), and North Africa/Middle East (AAPC 1.1%), with a decline in High-income North America. China had the highest incidence in 2021 (5.17 cases per 100,000), followed by Peru and Bolivia. Saudi Arabia had the highest DALYs attributable to EOPD. The decomposition study indicated that the rise in global incidence and DALYs from 1990 to 2021 was mainly driven by epidemiological changes and population growth, with regional variations in impact.ConclusionThis global analysis highlights the need for targeted interventions and research to address gender-specific risk factors, regional disparities, and the effects of epidemiological changes on the growing EOPD burden.
Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling
Authors: Chuang Cheng-Hung , Tai Yu-An , Wu Ting-Jing , Ho Ying-Jui , Yeh Shu-Lan 
Published: January 2025

IntroductionCancer-related fatigue (CRF) is a common symptom induced by chemotherapy. The main objective of the present study was to investigate whether quercetin regulates the hypothalamic-pituitary-adrenal (HPA) axis and chemoattractant protein-1 (MCP-1) signaling, two factors contributing to CRF in mice exposed to cisplatin.MethodsMale BALB/c mice were randomly assigned to the following five groups for 15 weeks: Control, CDDP, CDDP+TAK779 (an antagonist of MCP-1 receptor, human CC chemokine receptor R2 (CCR2)), CDDP+OQ (a diet containing 1% quercetin) and CDDP+IQ (quercetin given by ip, 10 mg/kg, 3 times/week).ResultsThe results first showed that OQ and IQ significantly increased grip strength and locomotor activity, decreased plasma cortisol/corticosterone levels, and decreased the corticotropin releasing hormone (CRH) mRNA level in the brain tissues in mice exposed to CDDP. OQ and IQ also decreased CDDP-induced plasma levels of MCP-1 as well as the mRNA expression of MCP-1 and CCR2 in the brain stem. TAK779 significantly increased grip strength and tended to decrease the cortisol/corticosterone levels in CDDP-exposed mice, indicating the association between the HPA axis and MCP-1 signaling.ConclusionTaken together, the study suggests that quercetin could attenuate CDDP-induced CRF through the mechanisms associated with downregulation of the HPA axis and MCP-1 signaling in mice.
Effects of dexmedetomidine-ropivacaine assisted combined spinal-epidural anesthesia on neutrophil-lymphocyte ratio and postoperative delirium in elderly patients with intertrochanteric femoral fracture
Authors: Bai Lili , Zhao Lina , Jia Fang , Liu Ying , Li Ping 
Published: January 2025

ObjectiveIntertrochanteric femoral fracture (IFF) is a public issue in the old. Combined spinal-epidural anesthesia (CSEA) is commonly utilized for lower limb orthopedic surgery in elderly patients. Therefore, this study explored the application of dexmedetomidine (Dex) and ropivacaine (Rop) assisted CSEA in elderly IFF patients.MethodsTotally 187 elderly IFF patients were assigned into the Rop assisted CSEA (Rop-CSEA), low-dose Dex-Rop assisted CSEA (low Dex and Rop-CSEA) and high-dose Dex-Rop assisted CSEA (high Dex and Rop-CSEA) groups. We compared block effects, hemodynamic indicators [heart rate (HR)/respiratory rate (RR)/mean arterial pressure (MAP)] at time before anesthesia (T0)/skin incision (T1)/10 min postoperatively (T2)/suture postoperatively (T3)/anesthesia recovery (T4), postoperative pain mediator release [substance P (SP)/prostaglandin E2 (PGE2)/5-hydroxytryptamine (5-HT)], neutrophil-lymphocyte ratio (NLR), adverse reactions, delirium and cognitive dysfunction incidence.ResultsCompared with the Rop-CSEA group, low/high Dex and Rop-CSEA groups had shortened onset times, prolonged recovery times in sensory/motor block, elevated HR/RR/MAP, repressed pain mediator release, and reduced postoperative delirium and cognitive dysfunction incidences. HR/RR/MAP exhibited reductions followed by elevations at T2-T4, and SP/PGE2/5-HT levels revealed elevations in all groups postoperatively. NLR level displayed enhancement followed by reduction, and NLR in the low/high Dex and Rop-CSEA groups was abated on postoperative days 1–5. Total incidence of adverse reactions in the high Dex and Rop-CSEA group was enhanced.ConclusionDex and Rop assisted CSEA shortens the onset time of anesthesia, maintains perioperative hemodynamic stability, inhibits pain mediator release, reduces postoperative NLR level and the incidence of delirium and cognitive dysfunction in IFF patients.
Soil chemical factors contributing to differences in bacterial communities among tea field soils and their relationships with tea quality
Authors: Zhang Shuning , Yanagisawa Naoki , Asahina Mio , Yamashita Hiroto , Ikka Takashi 
Published: January 2025

IntroductionSoil chemical properties and bacterial communities play key roles in shaping tea plant nutrient status and quality. While the relationships between soil bacterial communities and plant nutrient status have been investigated, the specific role by which soil bacterial communities interacted with soil properties to influence tea plant nutrients and quality remained underexplored.MethodsIn this study, different soil types were collected from tea gardens and designated as soil A (pH 3.41), soil B (pH 3.75), soil C (pH 4.16), soil D (pH 4.17) and soil E (pH 5.56) based on the initial soil pH. We conducted pot cultivation of tea plant ‘Yabukita’ to investigate how soil chemical factors affect bacterial communities and their influences on the nutrient status and quality of tea plants, and finally explored the complex relationships between soil bacterial features and tea quality.Results and discussionThe results showed that soil bacterial α-diversity was higher level in soils D and E, with distinct β-diversity patterns separating higher pH soils (D and E) from lower pH soils (A, B, and C). The dominant amplicon sequence variants (ASVs) in soils were Proteobacteria (28.12%), Actinobacteriota (25.65%), Firmicutes (9.99%) at phylum level, and Acidothermaceae (7.24%), Solirubrobacteraceae (4.85%), and Acetobacteraceae (4.50%) at family level. Soil pH, exchangeable Mg2+, and Ca2+ were identified as key factors shaping bacterial community composition and positively correlated with bacterial diversity. Differentially abundant ASVs (DAAs) among all soils were also identified including the phylum Firmicutes and families such as Paenibacillaceae, Alicyclobacillaceae, JG36-TzT-191, KF-JG30-C25, and Acidobacteriaceae_subgroup1. Besides, the nutrient content of tea new leaves varied significantly among soil types and harvests. Combined with Mantel-test association analysis, soil chemical properties and soil bacterial communities were jointly correlated with the contents of total nitrogen, potassium, calcium, aluminum, magnesium, free amino acids, and caffeine in tea new leaves. These findings highlight the dynamic interactions between soil properties, bacterial communities, and tea nutrients, emphasizing the importance of optimizing soil health and bacterial networks to improve tea quality.
Multidimensional evaluation of nutritional security policies in the Republic of Moldova: gaps, progress, and alignment with international standards
Authors: Siminiuc Rodica , Țurcanu Dinu , Siminiuc Sergiu 
Published: January 2025

IntroductionNutritional security is increasingly recognized as a critical component of public health, particularly in addressing the growing burden of non-communicable diseases such as obesity, diabetes, and cardiovascular disease. In the Republic of Moldova, rapid changes in food availability and consumption patterns have intensified the need for robust public policies to promote healthy dietary habits.MethodsThis study evaluates Moldova’s public policies on nutritional security using a multidimensional model adapted from the Policy Evaluation Network framework. The model assesses five key policy categories: educational, strategic, labeling, monitoring, and fiscal policies, through a comprehensive 28-indicator structure.ResultsThe findings indicate that Moldova’s policy efforts in nutritional security are at a “satisfactory” level, with foundational strategies in place but significant gaps in policy cohesion, implementation, and monitoring. Educational and strategic policies show moderate progress, yet labeling, monitoring, and fiscal policies are underdeveloped, limiting their impact on public health.DiscussionThe results emphasize the need for a more coordinated, multi-sectoral approach, incorporating targeted fiscal incentives, systematic monitoring frameworks, and comprehensive educational reforms.
Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis
Authors: Sa Rengeerli , Zhang Fengshuo , Zhang Xianhua , Gao Wei , Zhang Yu , Gan Jiacheng , Hou Shengzhen , Gui Linsheng 
Published: January 2025

IntroductionThis study employed lipidomics to investigate the effects of varying lysine (Lys)- to-methionine (Met) ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep fed a low-protein diet.MethodsNinety healthy male Tibetan sheep of similar body weight were randomly allocated into three groups. These sheep were fed a low-protein diet containing Lys/Met ratios of 1:1, 2:1, and 3:1. Ultra-High Performance Liquid Chromatography–tandem Mass Spectrometry (UHPLC–MS/MS) was employed to explore the changes in various lipid subclasses in subcutaneous adipose tissue. The expression of genes associated with adipogenesis, antioxidant capacity, and fatty acid metabolism was also examined.ResultsThe results indicated that the 1:1 Lys/Met group exhibited significantly higher antioxidant capacity (glutathione peroxidase, GSH-Px), with more orderly adipocyte arrangement, uniform cell size, and a general increase in unsaturated fatty acid levels. Additionally, several lipid molecules associated with the phenotype (Antioxidant index and fatty acid content) were identified, namely, DG(38:3e) + Na, PE(17:1_22:2)-H, PI(17:0_20:3)-H, TG(33:0e) + NH4, Cer(d14:0_17:1) + H, and CL(81:13)-2H. Furthermore, the findings showed that the upregulation of PPARγ, FASN, FAD4, CPT1A, and GPX4 can enhance adipocyte differentiation and lipid accumulation, thereby improving metabolic function in subcutaneous adipose tissue via the regulation of lipid metabolism and oxidative defense mechanisms.DiscussionIn summary, this study provides a theoretical foundation for optimizing precision feeding strategies for Tibetan sheep, offering crucial data to support enhancements in production efficiency and meat quality.
Effects of Deficit Irrigation and Intercropping of Dry Beans with Two African Leafy Vegetable Crops on Growth and Yield
Authors: Lekgoathi Boitumelo Patience , Kgopa Pholosho Mmateko , Soundy Puffy 
Published: January 2025

The instability between water demand and availability is making it challenging to cultivate viable, sustainable crops. To address this, improved irrigation scheduling regimes need to be adopted, focusing on full crop water requirements to determine optimal water allocation. This study aimed to identify suitable intercropping combinations of leguminous dry bean plants with indigenous African leafy vegetables (specifically cleome gynandra and cucumis myriocarpus) that could enhance interactions with regulated deficit irrigation. The experiment was designed in 3 (irrigation levels: 100%, 75%, and 50%) × 5 (three monocrops and two intercrops) split plot arrangement within a randomized complete block design (RCBD), replicated three times across four growth stages. The study was designed to rigorously assess the effectiveness of two intercrops compared to three monocrops, each cultivated under varying irrigation levels of 100%, 75%, and 50%. There was an increase in selected gaseous parameters, such as stomatal conductance and photosynthesis rates when dry beans were intercropped with cucumis myriocarpus and subjected to 75% deficit irrigation during the mid-pod stage of the dry bean. In contrast, decreased gaseous parameters were noted under a 50% deficit irrigation across all dry bean growth stages, which also negatively affected the growth of African leafy crops. Regarding yield, an inverse relationship was observed between the crops. The yield of dry beans significantly increased under 75% irrigation during the mid-pod stage. Meanwhile, yields of cucumis myriocarpus increased under both 75% and 100% irrigation levels during the early-pod stage of the dry beans, which corresponded to the vegetative stage of the two African leafy crops. In conclusion, regulating irrigation under 75% deficit irrigation during the mid-growth stage is recommended as an ideal irrigation level for intercropping dry beans with cucumis myriocarpus, and this can potentially save 25% of water.