bluuebunny/crossref_metadata_2025_split huggingface dataset:
Entangled harms: A reparative approach to climate justice
Authors: Petersmann Marie 
Published: February 2025

Abstract
Transnational climate litigation has become a strategic tool to press state and non-state actors into action. An analysis of international and domestic cases shows how rights and obligations are being materially, subjectively, spatially and temporally stretched in judicial proceedings. This article focuses on three distinct grammars of climate justice activated in climate litigation. The analysis exposes a shift from a traditional to a progressive grammar that moves from actual to potential climate harms, from human to nonhuman rights, from territorial to extra-territorial obligations, and from present to future generations. Beyond a traditional liberal framing of rights-based approaches to climate justice, we witness here a progressive critical grammar that broadens the scope of who can be considered legally affected by climate change, where, and how. A more radical understanding of climate justice, however, exceeds the capacity of these registers to confer structure, order, and meaning to climate harms across matter, subjects, space and time. Against this backdrop, a reparative grammar of climate justice is envisioned, which reconfigures the material boundary from potential to entangled harms, the subjective boundary from nonhuman victims to more-than-human care, the spatial boundary from extra-territorial to terrestrial spatiality, and the temporal boundary from future to enduring temporalities. In doing so, the analysis opens up a register of political thought for climate justice that starts in the law yet vastly exceeds and disrupts it.
Predictive utility of cardiac index-systemic vascular resistance plot and perfusion pressure in late post-Fontan patients
Authors: Kawasaki Yuki , Sasaki Takeshi , Kobayashi Daisuke 
Published: February 2025

Abstract

Introduction:
Cases of Fontan failure with normal Fontan pressure have been reported. This study aimed to identify catheterisation-derived haemodynamic predictors of heart transplantation/death, other than Fontan pressure, in late post-Fontan patients.

Methods:
This retrospective study evaluated post-Fontan patients who underwent cardiac catheterisation at age ≥10 years between 1993 and 2018. The predictive effect of cardiac index-systemic vascular resistance index plot and perfusion pressure on freedom from the primary outcome (heart transplantation/death) was evaluated. Patients were categorised into haemodynamic categories A (cardiac index ≥ 3, systemic vascular resistance index ≥ 13), B (cardiac index < 3, systemic vascular resistance index ≥ 13), C (cardiac index ≥ 3, systemic vascular resistance index < 13), and D (cardiac index < 3, systemic vascular resistance index < 13).

Results:
In total, 79 patients (median age: 15.7 [range: 10.1–50.2] years) were included; of them, the primary endpoint occurred in 10 (13%; median follow-up: 1.9 [range: 0.1–18.8] years). Category C patients had significantly shorter freedom from the endpoint than categories A and B patients. Univariate analysis identified significant haemodynamic predictors, including Fontan pressure, pulmonary/systemic vascular resistance index, pulmonary/systemic flow, systemic arterial oxygen saturation, systemic venous oxygen saturation, systemic vascular resistance index, perfusion pressure, perfusion pressure < 53 mmHg, and category C. In multivariable analysis, perfusion pressure < 53 mmHg and category C emerged as predictors of heart transplantation/death alongside Fontan pressure.

Conclusion:
Haemodynamic profiling of late post-Fontan patients using the cardiac index-systemic vascular resistance index plot can aid to comprehend the post-Fontan status and predict clinical prognosis.
How does skill distribution shape comparative advantage across industries? Theory and evidence
Authors: Li Zhongda , Liu Jieyi , Liu Lu 
Published: February 2025

Abstract
This paper revisits the role of skill distribution in shaping regional comparative advantage. Theoretically, we show that it is the relative skill dispersion between exporters and importers, rather than the absolute skill dispersion of exporters, that matters for the pattern of international trade. Using industry-level data on Chinese provincial export flows, we demonstrate that regions with a more dispersed skill distribution relative to their trading partners export more goods produced by sectors with lower skill complementarity. Exploring the potential mechanisms, we further find that the trade-promoting effect from relative skill dispersion probably operates through improving product quality and diversity.
Hume’s Scepticism and Kant’s Transcendental Deduction
Authors: Tulipana Paul , King Dustin 
Published: February 2025

Abstract
Kant’s aim in the Transcendental Deduction is to prove that the a priori categories of the understanding necessarily apply to objects of experience. He claims that he will do this simply by explaining how they could so apply. But the idea that a mere explanation of this possibility should provide a defence of the categories’ actual (let alone necessary) applicability is surprising. We argue that it can be understood by attending to the source of the scepticism that the Critique’s Analytic is supposed to overcome: Hume’s inability to explain causal knowledge in the Enquiry.
The HRAS-binding C2 domain of PLCη2 suppresses tumor‐like synoviocytes and experimental arthritis in rheumatoid arthritis
Authors: Jeon Hyun Min , Noh Hae Sook , Jeon Min-Gyu , Park Jin-Ho , Lee Young-Sun , Seo Gyunghwa , Cheon Yun-Hong , Kim Mingyo , Han Myung-Kwan , Park Jae-Yong , Lee Sang-Il 
Published: February 2025

Abstract
Fibroblast-like synoviocytes (FLSs), which are stromal cells that play key roles in rheumatoid arthritis (RA) pathophysiology, are characterized by a tumor-like phenotype and immunostimulatory actions. C2 domains in various proteins play roles in intracellular signaling and altering cellular characteristics, and some C2 domain-containing proteins exacerbate or alleviate certain malignant or inflammatory diseases. However, the roles of C2 domains in regulating the functions of RA FLSs remain unclear. Here we performed functional C2 domainomics with 144 C2 domain-containing viral vectors and identified the C2 domain of PLCη2 as a key regulator of RA FLSs. In mice, overexpressing PLCη2 or only its C2 domain PLCη2 (PLCη2_C2) diminished the proliferation, migration, invasion and inflammatory responses of RA FLSs, mitigating RA pathology; the absence of PLCη2 amplified these proinflammatory and destructive processes in RA FLSs in vivo. Mechanistically, PLCη2 and PLCη2_C2 participate in the pathological signaling of RA FLSs in a calcium-independent manner through protein–protein interactions. Specifically, PLCη2_C2 disrupted HRAS–RAF1 interactions, suppressing downstream signaling pathways, including the NF-κB, JAK–STAT and MAPK pathways. Collectively, these findings establish PLCη2 and PLCη2_C2 as novel inhibitory regulators in RA, suggesting promising therapeutic avenues for addressing FLS-driven disease mechanisms.
Development and validation of a prediction model for bronchopulmonary dysplasia using respiratory severity score
Authors: Kanzawa Takahiro , Kinoshita Fumie , Namba Fumihiko , Tanaka Taihei , Oshiro Makoto , Sugiura Takahiro , Kato Yuichi , Miyata Masafumi , Yamada Yasumasa , Iwata Osuke , Hayakawa Masahiro , Sato Yoshiaki ,   BPD Prediction Model Collaborative Clinical Research Team, Hattori Tetsuo , Boda Hiroko , Fujino Masayuki , Kawai Yuri , Kojima Arisa , Manabe Masahiko , Nakauchi Chiharuko , Funato Yusuke , Kamino Shigemitsu , Tsuda Kennosuke , Kato Shin , Muratmatsu Kanji , Haga Mitsuhiro , Konishi Asami , Noda Haruka , Shinohara Osamu , Hayashi Seiji , Murai Yuko , Ieda Kuniko , Honbe Kazuya , Asai Masami , Nagasaki Rika , Yamamoto Hikaru , Yamada Midori , Takemoto Koji , Nagaya Yoshiaki , Yamamoto Kazuyuki , Yasuda Kazushi , Kawai Satoru , Yokoyama Takehiko , Hamasaki Sayako , Fujishiro Naozumi , Tanaka Ryo ,   the Neonatal Research Network of Japan
Published: February 2025

Abstract

Background
To develop and validate a prediction model for severe bronchopulmonary dysplasia (BPD) that integrates the respiratory severity (RS) score with early postnatal risk factors.

Methods
This retrospective cohort study included preterm infants born at less than 32 weeks gestation or with a birth weight of less than 1500 g, from Aichi Prefecture (training dataset) and Saitama Medical University (validation dataset) from April 1, 2016, to March 31, 2020. The primary outcome was severe BPD, defined as the use of home oxygen therapy or death due to BPD. We used classification and regression tree (CART) analysis to explore the relationship between outcomes and BPD risk factors in the training dataset.

Results
The incidence of severe BPD was 149 out of 2026 (7.3%) in the training dataset and 35 out of 387 (8.9%) in the validation dataset. CART analysis identified gestational age and the RS score as significant predictors of outcome in the day 7 and day 14 models, with C-statistics of 0.789 and 0.779, respectively. When applied to the validation dataset, these models achieved C-statistics of 0.753 and 0.827, respectively.

Conclusion
Our prediction models demonstrated the ability to predict severe BPD, with the RS score being a crucial predictor.

Impact

Many existing prediction models for bronchopulmonary dysplasia (BPD) use multiple predictors, and do not provide specific cutoff values, which complicates their clinical application.

To address this issue, we developed a prediction model for severe BPD based on a score derived from mean airway pressure and inhaled oxygen concentration at 1–2 weeks of age.

This user-friendly model can be easily integrated into clinical practice, facilitating treatment decisions based on predicted probabilities.
Regenerative potential of concentrated growth factor compared to platelet-rich fibrin in treatment of necrotic mature teeth: a randomized clinical trial
Authors: Salah Taghreed , Hussein Wael , Abdelkafy Heba 
Published: February 2025

Abstract

Aim
This study aimed to compare the potential of two regenerative endodontic procedures, platelet-rich fibrin (PRF) and concentrated growth factor (CGF), in the treatment of mature permanent teeth with necrotic pulps and periapical radiolucency.

Methods
This randomized clinical trial was written according to the Preferred Reporting Items for Randomized Trials in Endodontics 2020 guidelines. The study was registered in the Clinical Trials Registry (ClinicalTrials.gov) with identifier number NCT06227533. Eighteen patients with mature necrotic maxillary incisors with periapical lesions were randomly divided into two groups, one group received treatment with PRF (n = 9), while the other received CGF (n = 9). Radiographs were taken at the beginning, 6 and 12 months after treatment, and confirmed with CBCT to assess periradicular healing. An electric pulp tester and cold test were used to determine whether pulp sensibility had been regained during the follow-up period. The statistical analysis used an independent t-test to compare different groups. Repeated measures ANOVA to compare pre-operative, after 6 months, and after 1 year in the same group.

Results
Both groups experienced a considerable increase in healing at 6 and 12 months compared to baseline, with no significant differences seen regarding lesion size (p-value was 0.34) and relative bone density (p-value was 0.27). There was no substantial change in the tooth sensibility reading between both groups at 6 months (p-value = 0.34), After 1 year, both groups exhibited identical patterns.

Conclusion
Revascularization using PRF or CGF successfully preserved mature teeth with necrotic pulps.
Selective peptide bond formation via side chain reactivity and self-assembly of abiotic phosphates
Authors: Sharma Arti , Dai Kun , Pol Mahesh D. , Thomann Ralf , Thomann Yi , Roy Subhra Kanti , Pappas Charalampos G. 
Published: February 2025

Abstract
In the realm of biology, peptide bonds are formed via reactive phosphate-containing intermediates, facilitated by compartmentalized environments that ensure precise coupling and folding. Herein, we use aminoacyl phosphate esters, synthetic counterparts of biological aminoacyl adenylates, that drive selective peptide bond formation through side chain-controlled reactivity and self-assembly. This strategy results in the preferential incorporation of positively charged amino acids from mixtures containing natural and non-natural amino acids during the spontaneous formation of amide bonds in water. Conversely, aminoacyl phosphate esters that lack assembly and exhibit fast reactivity result in random peptide coupling. By introducing structural modifications to the phosphate esters (ethyl vs. phenyl) while retaining aggregation, we are able to tune the selectivity by incorporating aromatic amino acid residues. This approach enables the synthesis of sequences tailored to the specific phosphate esters, overcoming limitations posed by certain amino acid combinations. Furthermore, we demonstrate that a balance between electrostatic and aromatic stacking interactions facilitates covalent self-sorting or co-assembly during oligomerization reactions using unprotected N-terminus aminoacyl phosphate esters. These findings suggest that self-assembly of abiotic aminoacyl phosphate esters can activate a selection mechanism enabling the departure from randomness during the autonomous formation of amide bonds in water.
Constraining the equation of state in neutron-star cores via the long-ringdown signal
Authors: Ecker Christian , Gorda Tyler , Kurkela Aleksi , Rezzolla Luciano 
Published: February 2025

Abstract
Multimessenger signals from binary neutron star (BNS) mergers are promising tools to infer the properties of nuclear matter at densities inaccessible to laboratory experiments. Gravitational waves (GWs) from BNS merger remnants can constrain the neutron-star equation of state (EOS) complementing constraints from late inspiral, direct mass-radius measurements, and ab-initio calculations. We perform a series of general-relativistic simulations of BNS systems with EOSs constructed to comprehensively cover the high-density regime. We identify a tight correlation between the ratio of the energy and angular-momentum losses in the late-time portion of the post-merger signal, called the long ringdown, and the EOS at the highest pressures and densities in neutron-star cores. Applying this correlation to post-merger GW signals significantly reduces EOS uncertainty at densities several times the nuclear saturation density, where no direct constraints are currently available. Hence, the long ringdown can provide stringent constraints on material properties of neutron stars cores.
RECQ4-MUS81 interaction contributes to telomere maintenance with implications to Rothmund-Thomson syndrome
Authors: Ashraf Raghib , Polasek-Sedlackova Hana , Marini Victoria , Prochazkova Jana , Hasanova Zdenka , Zacpalova Magdalena , Boudova Michala , Krejci Lumir 
Published: February 2025

Abstract
Replication stress, particularly in hard-to-replicate regions such as telomeres and centromeres, leads to the accumulation of replication intermediates that must be processed to ensure proper chromosome segregation. In this study, we identify a critical role for the interaction between RECQ4 and MUS81 in managing such stress. We show that RECQ4 physically interacts with MUS81, targeting it to specific DNA substrates and enhancing its endonuclease activity. Loss of this interaction, results in significant chromosomal segregation defects, including the accumulation of micronuclei, anaphase bridges, and ultrafine bridges (UFBs). Our data further demonstrate that the RECQ4-MUS81 interaction plays an important role in ALT-positive cells, where MUS81 foci primarily colocalise with telomeres, highlighting its role in telomere maintenance. We also observe that a mutation associated with Rothmund-Thomson syndrome, which produces a truncated RECQ4 unable to interact with MUS81, recapitulates these chromosome instability phenotypes. This underscores the importance of RECQ4-MUS81 in safeguarding genome integrity and suggests potential implications for human disease. Our findings demonstrate the RECQ4-MUS81 interaction as a key mechanism in alleviating replication stress at hard-to-replicate regions and highlight its relevance in pathological conditions such as RTS.
Large-angle Lorentz Four-dimensional scanning transmission electron microscopy for simultaneous local magnetization, strain and structure mapping
Authors: Kang Sangjun , Töllner Maximilian , Wang Di , Minnert Christian , Durst Karsten , Caron Arnaud , Dunin-Borkowski Rafal E. , McCord Jeffrey , Kübel Christian , Mu Xiaoke 
Published: February 2025

Abstract
Small adjustments in atomic configurations can significantly impact the magnetic properties of matter. Strain, for instance, can alter magnetic anisotropy and enable fine-tuning of magnetism. However, the effects of these changes on nanoscale magnetism remain largely unexplored. In particular, when strain fluctuates at the nanoscale, directly linking structural changes with magnetic behavior poses a substantial challenge. Here, we develop an approach, LA-Ltz-4D-STEM, to map structural information and magnetic fields simultaneously at the nanoscale. This approach opens avenues for an in-depth study of structure-property correlations of magnetic materials at the nanoscale. We applied LA-Ltz-4D-STEM to image strain, atomic packing, and magnetic fields simultaneously in a deformed amorphous ferromagnet with complex strain variations at the nanoscale. An anomalous magnetic configuration near shear bands, which reside in a magnetostatically high-energy state, was observed. By performing pixel-to-pixel correlation of the different physical quantities across a large field of view, a critical aspect for investigating industrial ferromagnetic materials, the magnetic moments were classified into two distinct groups: one influenced by magnetoelastic coupling and the other oriented by competition with magnetostatic energy.
Nature-inspired platform nanotechnology for RNA delivery to myeloid cells and their bone marrow progenitors
Authors: Hofstraat Stijn R. J. , Anbergen Tom , Zwolsman Robby , Deckers Jeroen , van Elsas Yuri , Trines Mirre M. , Versteeg Iris , Hoorn Daniek , Ros Gijs W. B. , Bartelet Branca M. , Hendrikx Merel M. A. , Darwish Youssef B. , Kleuskens Teun , Borges Francisca , Maas Rianne J. F. , Verhalle Lars M. , Tielemans Willem , Vader Pieter , de Jong Olivier G. , Tabaglio Tommaso , Wee Dave Keng Boon , Teunissen Abraham J. P. , Brechbühl Eliane , Janssen Henk M. , Fransen P. Michel , de Dreu Anne , Schrijver David P. , Priem Bram , Toner Yohana C. , Beldman Thijs J. , Netea Mihai G. , Mulder Willem J. M. , Kluza Ewelina , van der Meel Roy 
Published: February 2025

Abstract
Nucleic acid therapeutics are used for silencing, expressing or editing genes in vivo. However, their systemic stability and targeted delivery to bone marrow resident cells remains a challenge. In this study we present a nanotechnology platform based on natural lipoproteins, designed for delivering small interfering RNA (siRNA), antisense oligonucleotides and messenger RNA to myeloid cells and haematopoietic stem and progenitor cells in the bone marrow. We developed a prototype apolipoprotein nanoparticle (aNP) that stably incorporates siRNA into its core. We then created a comprehensive library of aNP formulations and extensively characterized their physicochemical properties and in vitro performance. From this library, we selected eight representative aNP-siRNA formulations and evaluated their ability to silence lysosomal-associated membrane protein 1 (Lamp1) expression in immune cell subsets in mice after intravenous administration. Using the most effective aNP identified from the screening process, we tested the platform’s potential for therapeutic gene silencing in a syngeneic murine tumour model. We also demonstrated the aNP platform’s suitability for splice-switching with antisense oligonucleotides and for protein production with messenger RNA by myeloid progenitor cells in the bone marrow. Our data indicate that the aNP platform holds translational potential for delivering various types of nucleic acid therapeutics to myeloid cells and their progenitors.
Environmental and health risk assessment of polycyclic aromatic hydrocarbons and toxic elements in the red sea using Monte Carlo simulation
Authors: Sayed F. Alshaima , Eid Mohamed Hamdy , El-Sherbeeny Ahmed M. , Abdel-Gawad Gouda Ismail , Mohamed Essam A. , Abukhadra Mostafa R. 
Published: February 2025

Abstract
This research evaluates the environmental and health risks linked to potentially toxic elements (PTEs) and PAHs along the western coast of the Gulf of Suez, Egypt. This study investigated the concentration of 16 PAH compounds in the Suez Gulf, revealing significantly higher levels than the EU (0.20 µg/L) and US (0.030 µg/L) standards. The average total PAH concentration across eight locations was significantly higher, with the Suez area having the highest concentration at 479 µg/L. Pyrene (Pyr) was the dominant PAH with a concentration of 443 µg/L in Suez, while acenaphthylene (Ace) had the lowest concentration at 0.120 µg/L in Northern Zaafarana. Carcinogenic PAHs (CAR) ranged from 8.67 µg/L at Ras Gharib to 29.62 µg/L at Suez, highlighting the urgent need for regulatory measures. Confirmatory ratios pointed to industrial and shipping influences as petrogenic sources. Elevated total organic carbon (TOC) levels in Suez Bay indicated aggravated organic pollution, exacerbated by oil rigs and refineries. The ecological risk assessment highlighted substantial risks, particularly in Suez, necessitating immediate interventions to combat PAH contamination and preserve the environmental balance of the Red Sea. The dominant metals in water samples were arranged in descending order as follows: Pb > Fe > Cr > Cu > Zn > Mn > Cd > Ni. The study evaluated environmental and human health risks using a multifaceted approach, including cluster analysis, principal component analysis, and various indices (HPI, RI, MI, HQ, HI, and CR). Most water samples exhibited high pollution risks, surpassing permissible limits for HPI (> 100) and MI (> 6). Notably, HI oral values indicated significant non-carcinogenic risks for adults and children. While HI values for adults suggested low-risk dermal contact, those for children showed a substantial proportion in the high-risk category. Most water samples displayed CR values exceeding 1 × 10–4 for Cd, Cr, and Pb, indicating vulnerability to carcinogenic effects in both age groups. Monte Carlo simulations reinforced these findings, revealing a significant carcinogenic impact on children and adults. The identified clusters, reflective of industrial, petroleum-related, and urban runoff contamination sources, were consistently validated and clarified through PCA, enhancing the reliability of the findings. In light of these results, urgent and comprehensive water treatment measures are imperative to mitigate carcinogenic and non-carcinogenic health risks. These insights provide a foundation for implementing targeted management strategies to effectively address the challenges of heavy metal contamination in the Red Sea.