bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of physical activity changes on exercise capacity and health-related quality of life in young patients with CHD: a 3-year follow-up study
Authors: Kim Hyun Jeong , Yoon Ja-kyoung , Cho Min Jeong , Kunutsor Setor K. , Kim Seong-Ho , Jae Sae Young 
Published: December 2023

AbstractObjective:This study examined the relationship between changes in physical activity and their impact on exercise capacity and health-related quality of life over a 3-year span in patients with CHD.Methods:We evaluated 99 young patients with CHD, aged 13–18 years at the outset. Physical activity, health-related quality of life, and exercise capacity were assessed via questionnaires and peak oxygen uptake measurements at baseline and after 3 years; changes in measures were estimated between the two time points and categorised into quartiles. Participants were stratified according to achieved (active) or not-achieved (inactive) recommended levels of physical activity (≥150 minutes/week) at both time points.Results:Despite increases in physical activity, exercise capacity, and health-related quality of life over 3 years, the changes were not statistically significant (all p > 0.05). However, a positive association was found between physical activity changes and exercise capacity (ß = 0.250, p = 0.040) and health-related quality of life improvements (ß = 0.380, p < 0.001). Those with the most pronounced physical activity increase showed notable exercise capacity (p < 0.001) and health-related quality of life increases (p < 0.001) compared with patients with the largest decline in physical activity. The active-inactive category demonstrated a notable decline in exercise capacity compared to the active-active group, while the inactive-active group showed health-related quality of life improvements.Conclusions:Over 3 years, increased physical activity was consistently linked to increases in exercise capacity and health-related quality of life in patients with CHD, highlighting the potential of physical activity augmentation as an intervention strategy.
Evaluation of quality of life and parental attitudes in patients who underwent atrial septal defect closure in childhood
Authors: Torunoğlu Bek Zeynep , Oğuz A. Deniz , Soysal Acar A. Şebnem , Terlemez Semiha , Canbeyli Fatma Hayvacı , Kula Serdar , Tunaoğlu Fatma Sedef 
Published: December 2023

AbstractObjective:In this study, we aimed to evaluate quality of life and parental attitudes in children who underwent an atrial septal defect closure procedure with a transcatheter or surgical method in childhood and whether they continued their lives with similar activities to their healthy peers by comparing the two groups.Methods:Patient forms to define sociodemographic and clinical features, the Questionnaire for Measuring Health-Related Quality of Life in Children and Adolescents (KINDL) to measure the quality of life of children, and the Parent-Child Relationship Test (Parental Attitude Research Instrument) to measure parental attitudes were used.Results:The groups were similar in terms of age and sex. The mean quality of life scale scores were high in all groups, and there was no statistically significant difference between the scores. Parents of the patients who underwent closure received higher scores from the demographic attitudes and recognition of quality subdimension compared to the parents of the healthy group.Conclusions:The quality of life of children with atrial septal defect closure was found to be similar to their healthy peers. Additionally, the effects of surgical or percutaneous closure of atrial septal defect on quality of life are similar. Children with atrial septal defect closure perceive their health status as well as their healthy peers, and this perception does not cause any difference in the attitudes and behaviours of families.
Dual-band transmissive linear to circular polarization converter with angular-stable and orthogonal polarizations
Authors: Zhang Bianmei , Wang Chen , Yu Shuo , Yang Xiaofan , Fang Zhibin , Liu Xiaoming 
Published: December 2023

AbstractA dual-band angular-stable transmissive linear to circular polarization converter based on metasurface is proposed and demonstrated in this work. The converter consists of three layers. The top and bottom layers are formed by an array of double split-ring layers. The unit cell of the central layer contains a square loop nesting a slant dipole. The split-rings create two resonances, enabling dual-band operation. The slant dipole and square loop are useful for improving the quality of circular polarization conversion. It is shown that the proposed polarization converter converts the incident linearly polarized wave into circularly polarized wave with opposite polarization modes over the frequency ranges of 8.77–10.58 and 17.59–19.88 GHz. The angular stability is up to 60° for 3 dB axial ratio. Moreover, the thickness of unit cell has a wavelength below 0.06 at the lower band. Compared with other designs in the literature, the structure bears merits of wideband response, high angular stability, and low-profile property within dual-band operational region. To validate the design, a sample prototype was designed, fabricated, and measured. The measured results are in good agreement with the simulated ones.
Location and Timing of Magnetic Reconnections in Earth's Magnetotail: Accomplishments of the 29‐Year Geotail Near‐Earth Magnetotail Survey
Authors: Nagai T. , Shinohara I. , Saito Y. , Ieda A. , Nakamura R. 
Published: December 2023

AbstractThe spacecraft Geotail surveyed the near‐Earth plasma sheet from XGSM = −10 to −31 RE and YGSM = −20 to +20 RE during the period from 1994 to 2022. It observed 243 magnetic reconnection events and 785 tailward flow events under various solar wind conditions during plasma sheet residence time of over 23,000 hr. Magnetic reconnections associated with the onset of magnetospheric substorms occur mostly in the range XGSM = −23 to −31 RE. When the solar wind is intense and high substorm activities continue, magnetic reconnection can occur closer to the Earth. The YGSM locations of magnetic reconnections depend on the solar wind conditions and on previous substorm activity. Under normal solar wind conditions, magnetic reconnection occurs preferentially in the pre‐midnight plasma sheet. Under conditions with intense (weak) solar wind energy input, however, magnetic reconnection can occur in the post‐midnight (duskside) plasma sheet. Continuous substorm activity tends to shift the magnetic reconnection site duskward. The plasma sheet thinning proceeds faster under intense solar wind conditions, and the loading process that provides the preconditions for magnetic reconnection becomes shorter. When magnetic flux piles up during a prolonged period with a strongly northward‐oriented interplanetary magnetic field (IMF) Bz, the time necessary to provide the preconditions for magnetic reconnection becomes longer. Although the solar wind conditions are the primary factors that control the location and timing of magnetic reconnections, the plasma sheet conditions created by preceding substorm activity or the strongly northward IMF Bz can modify the solar wind control.
Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate
Authors: Asparuhova Maria B. , Song Xiaoqing , Riedwyl Dominic , van Geest Geert , Bosshardt Dieter D. , Sculean Anton 
Published: December 2023

AbstractThe present study aimed to assess the molecular profiles of subepithelial connective tissue grafts (CTGs) obtained at different locations and depths in the human palate. Sixty-four CTGs belonging to anterior deep (AD), anterior superficial (AS), posterior deep (PD), and posterior superficial (PS) groups were subjected to RNA-Sequencing and their transcriptomes were analyzed computationally. Functional correlations characterizing the CTG groups were validated by cell biological experiments using primary human palatal fibroblasts (HPFs) extracted from the CTGs. A clearly more pronounced location-dependent than depth-dependent difference between the grafts, with a minimal number of genes (4) showing no dependence on the location, was revealed. Epithelial, endothelial, and monocytic cell migration was strongly (P < 0.001) potentiated by AD- and PS-HPFs. Moreover, significantly increased expression of genes encoding C-C and C-X-C motif chemokine ligands as well as significantly (P < 0.01) activated p38 signaling suggested immunomodulatory phenotype for AD- and PS-HPFs. Increased growth factor gene expression and significantly activated (P < 0.001) Erk and Akt signaling in HPFs originating from A-CTGs implied their involvement in cell survival, proliferation, and motility. Prominent collagen-rich expression profile contributing to high mechanical stability, increased osteogenesis-related gene expression, and strongly activated (P < 0.001) Smad1/5/8 signaling characterized HPFs originating from P-CTGs. The present data indicate that in humans, differences between palatal CTGs harvested from different locations and depths appear to be location- rather than depth-dependent. Our findings provide the basis for future personalization of the therapeutic strategy by selecting an optimal graft type depending on the clinical indications.
HOMER3 promotes non-small cell lung cancer growth and metastasis primarily through GABPB1-mediated mitochondrial metabolism
Authors: Sun Teng , Song Chao , Zhao Guoqing , Feng Shoujie , Wei Jianhao , Zhang Lixia , Liu Xiangming , Li Zhuoqun , Zhang Hao 
Published: December 2023

AbstractCancer metabolism has emerged as a major target for cancer therapy, while the state of mitochondrial drugs has remained largely unexplored, partly due to an inadequate understanding of various mitochondrial functions in tumor contexts. Here, we report that HOMER3 is highly expressed in non-small cell lung cancer (NSCLC) and is closely correlated with poor prognosis. Lung cancer cells with low levels of HOMER3 are found to show significant mitochondrial dysfunction, thereby suppressing their proliferation and metastasis in vivo and in vitro. At the mechanistic level, we demonstrate that HOMER3 and platelet-activating factor acetylhydrolase 1b catalytic subunit 3 cooperate to upregulate the level of GA-binding protein subunit beta-1 (GABPB1), a key transcription factor involved in mitochondrial biogenesis, to control mitochondrial inner membrane genes and mitochondrial function. Concurrently, low levels of HOMER3 and its downstream target GABPB1 led to mitochondrial dysfunction and decreased proliferation and invasive activity of lung cancer cells, which raises the possibility that targeting mitochondrial synthesis is an important and promising therapeutic approach for NSCLC.
Mint3-depletion-induced energy stress sensitizes triple-negative breast cancer to chemotherapy via HSF1 inactivation
Authors: Tanaka Noritaka , Okada Hikari , Yamaguchi Kiyoshi , Seki Masahide , Matsubara Daisuke , Gotoh Noriko , Suzuki Yutaka , Furukawa Yoichi , Yamashita Taro , Inoue Jun-ichiro , Kaneko Shuichi , Sakamoto Takeharu 
Published: December 2023

AbstractGiven the lack of therapeutic targets, the conventional approach for managing triple-negative breast cancer (TNBC) involves the utilization of cytotoxic chemotherapeutic agents. However, most TNBCs acquire resistance to chemotherapy, thereby lowering the therapeutic outcome. In addition to oncogenic mutations in TNBC, microenvironment-induced mechanisms render chemoresistance more complex and robust in vivo. Here, we aimed to analyze whether depletion of Munc18-1 interacting protein 3 (Mint3), which activates hypoxia-inducible factor 1 (HIF-1) during normoxia, sensitizes TNBC to chemotherapy. We found that Mint3 promotes the chemoresistance of TNBC in vivo. Mint3 depletion did not affect the sensitivity of human TNBC cell lines to doxorubicin and paclitaxel in vitro but sensitized tumors of these cells to chemotherapy in vivo. Transcriptome analyses revealed that the Mint3–HIF-1 axis enhanced heat shock protein 70 (HSP70) expression in tumors of TNBC cells. Administering an HSP70 inhibitor enhanced the antitumor activity of doxorubicin in TNBC tumors, similar to Mint3 depletion. Mint3 expression was also correlated with HSP70 expression in human TNBC specimens. Mechanistically, Mint3 depletion induces glycolytic maladaptation to the tumor microenvironment in TNBC tumors, resulting in energy stress. This energy stress by Mint3 depletion inactivated heat shock factor 1 (HSF-1), the master regulator of HSP expression, via the AMP-activated protein kinase/mechanistic target of the rapamycin pathway following attenuated HSP70 expression. In conclusion, Mint3 is a unique regulator of TNBC chemoresistance in vivo via metabolic adaptation to the tumor microenvironment, and a combination of Mint3 inhibition and chemotherapy may be a good strategy for TNBC treatment.
RIF1 regulates early replication timing in murine B cells
Authors: Malzl Daniel , Peycheva Mihaela , Rahjouei Ali , Gnan Stefano , Klein Kyle N. , Nazarova Mariia , Schoeberl Ursula E. , Gilbert David M. , Buonomo Sara C. B. , Di Virgilio Michela , Neumann Tobias , Pavri Rushad 
Published: December 2023

AbstractThe mammalian DNA replication timing (RT) program is crucial for the proper functioning and integrity of the genome. The best-known mechanism for controlling RT is the suppression of late origins of replication in heterochromatin by RIF1. Here, we report that in antigen-activated, hypermutating murine B lymphocytes, RIF1 binds predominantly to early-replicating active chromatin and promotes early replication, but plays a minor role in regulating replication origin activity, gene expression and genome organization in B cells. Furthermore, we find that RIF1 functions in a complementary and non-epistatic manner with minichromosome maintenance (MCM) proteins to establish early RT signatures genome-wide and, specifically, to ensure the early replication of highly transcribed genes. These findings reveal additional layers of regulation within the B cell RT program, driven by the coordinated activity of RIF1 and MCM proteins.
GRAF1 integrates PINK1-Parkin signaling and actin dynamics to mediate cardiac mitochondrial homeostasis
Authors: Zhu Qiang , Combs Matthew E. , Liu Juan , Bai Xue , Wang Wenbo B. , Herring Laura E. , Liu Jiandong , Locasale Jason W. , Bowles Dawn E. , Gross Ryan T. , Pla Michelle Mendiola , Mack Christopher P. , Taylor Joan M. 
Published: December 2023

AbstractThe serine/threonine kinase, PINK1, and the E3 ubiquitin ligase, Parkin, are known to facilitate LC3-dependent autophagosomal encasement and lysosomal clearance of dysfunctional mitochondria, and defects in this process contribute to a variety of cardiometabolic and neurological diseases. Although recent evidence indicates that dynamic actin remodeling plays an important role in PINK1/Parkin-mediated mitochondrial autophagy (mitophagy), the underlying signaling mechanisms remain unknown. Here, we identify the RhoGAP GRAF1 (Arhgap26) as a PINK1 substrate that regulates mitophagy. GRAF1 promotes the release of damaged mitochondria from F-actin anchors, regulates mitochondrial-associated Arp2/3-mediated actin remodeling and facilitates Parkin-LC3 interactions to enhance mitochondria capture by autophagosomes. Graf1 phosphorylation on PINK1-dependent sites is dysregulated in human heart failure, and cardiomyocyte-restricted Graf1 depletion in mice blunts mitochondrial clearance and attenuates compensatory metabolic adaptations to stress. Overall, we identify GRAF1 as an enzyme that coordinates cytoskeletal and metabolic remodeling to promote cardioprotection.
Elucidation of the structural basis for ligand binding and translocation in conserved insect odorant receptor co-receptors
Authors: Pacalon Jody , Audic Guillaume , Magnat Justine , Philip Manon , Golebiowski Jérôme , Moreau Christophe J. , Topin Jérémie 
Published: December 2023

AbstractIn numerous insects, the olfactory receptor family forms a unique class of heteromeric cation channels. Recent progress in resolving the odorant receptor structures offers unprecedented opportunities for deciphering their molecular mechanisms of ligand recognition. Unexpectedly, these structures in apo or ligand-bound states did not reveal the pathway taken by the ligands between the extracellular space and the deep internal cavities. By combining molecular modeling with electrophysiological recordings, we identified amino acids involved in the dynamic entry pathway and the binding of VUAA1 to Drosophila melanogaster’s odorant receptor co-receptor (Orco). Our results provide evidence for the exact location of the agonist binding site and a detailed and original mechanism of ligand translocation controlled by a network of conserved residues. These findings would explain the particularly high selectivity of Orcos for their ligands.
Effect of activated carbon microstructure and adsorption mechanism on the efficient removal of chlorophyll a and chlorophyll b from Andrographis paniculata extract
Authors: Liang Di , Ji Bao-yu , Wang Yun , Li Xia , Gao Wen-Yuan 
Published: December 2023

AbstractIn order to reveal the effect of activated carbon (AC) properties on the adsorption of chlorophyll a (Chl a) and chlorophyll b (Chl b) in Andrographis paniculata extract, four commercial activated carbons were first tested and characterized. The results showed that activated carbon 1 (AC1) had the best surface area, pore structure and adsorption capacity. Therefore, adsorption isotherms, adsorption kinetics and adsorption mechanism were further carried out on AC1. The application of Langmuir model (R2 > 0.978) and Freundlich model (R2 > 0.977) indicated that the adsorption process of Chl a and Chl b on AC1 may be a complex adsorption process of single-layer and multilayer adsorption. The adsorption kinetics indicated that the pseudo-second-order kinetic model (R2 > 0.999) was dominant and was mainly chemisorption. The intra-particle diffusion model (R2 > 0.937) shows that the intra-particle diffusion is the rate-limiting step. The decrease of adsorption of AC1 to Chl a and Chl b due to the oxidation of acrylic acid proves the importance of π–π interaction.
F﻿irst detection ﻿of herpesvirus and hemosporidians in the endangered Pyrenean Capercaillie (Tetrao urogallus aquitanicus)
Authors: de Francisco Olga Nicolás , Sacristán Irene , Ewbank Ana Carolina , Velarde Roser , Afonso Ivan , Garcia-Ferré Diego , Martín-Maldonado Bárbara , Esperón Fernando , Iglesias Irene , de la Torre Ana , Margalida Antoni , Sacristán Carlos 
Published: December 2023

AbstractPathogens affect wild bird populations worldwide, contributing to their decline. Considering the scarce health data regarding the endangered Pyrenean Capercaillie (Tetrao urogallus aquitanicus), we molecularly surveyed selected pathogens (Newcastle disease virus, Avian influenza virus, Chlamydia psittaci, avian pathogenic Escherichia coli, Campylobacter jejuni, and Salmonella spp.) in 30 Pyrenean Capercaillie feces collected in the field (Catalonia, northeastern Spain). Additionally, swab and tissue samples from eight wild Pyrenean Capercaillies of Catalonia and Andorra were molecularly tested for herpesvirus and hemosporidians (Plasmodium spp., Haemoproteus spp., and Leucocytozoon spp.). All fecal samples were negative for the pathogens tested. Nevertheless, we detected a novel herpesvirus in 50% (4/8) of the Pyrenean Capercaillies, and hemosporidian DNA in 62.5% (5/8) of the tissue samples (i.e., Haemoproteus sp. [4 of 8] and/or Leucocytozoon sp. [3 of 8]). To our knowledge, this is the first detection of herpesvirus and hemosporidians infections in Pyrenean Capercaillies. The putative novel herpesvirus belongs to the genus Iltovirus. The presence of hemosporidian parasites in this mountain bird species is of concern, and could be related to the marked increase in the average temperature in the Pyrenees as a consequence of climate change. Our findings are fundamental to improve the conservation plans for the endangered Pyrenean Capercaillie population.
A multiscale assessment of bowel impairment in an Italian multiple sclerosis cohort
Authors: Tavazzi Eleonora , Zito Antonio , Montomoli Cristina , Bergsland Niels , Colombo Elena , La Malfa Alessandro , Bergamaschi Roberto 
Published: December 2023

AbstractBowel dysfunctions (BD) in multiple sclerosis (MS) are under reported despite their clinical relevance. Scales usually applied do not thoroughly assess constipation and fecal incontinence. Instead, a proper qualitative and quantitative description of these symptoms might have relevant clinical and scientific consequences. The aim of this project is to study the prevalence of BD in a cohort of persons with MS (pwMS). Four-hundred and forty-seven pwMS (330 relapsing–remitting MS-RRMS and 117 progressive MS-PMS) were recruited. Three different questionnaires were administered: the neurogenic bowel dysfunction score (NBDS), the Wexner constipation scale (WexCon) and the Wexner incontinence scale (WexInc). All the scales were divided in subscores according to symptom severity. The prevalence of BD, considered as NBDS > 0, was 53.7%. Mean scores in pwMS group were as follows: NBDS 2.6 (SD 3.5), WexInc 1.1 (SD 2.4), WexCon 4.4 (SD 5.9). NBDS, WexCon and WexInc were significantly higher in PMS vs RRMS (p < 0.001), and significantly associated with disease duration, EDSS, multiple sclerosis severity score (p < 0.001), as well as with each other (p < 0.001). Our study confirms the presence of bowel dysfunctions in a large group of pwMS with a wide range of disability and their association with progressive disease phenotype and clinical disability.
Genetic analysis of chorionic villus tissues in early missed abortions
Authors: Xue Huili , Guo Qun , Yu Aili , Lin Min , Chen Xuemei , Xu Liangpu 
Published: December 2023

AbstractChromosomal abnormalities are the most common etiology of early spontaneous miscarriage. However, traditional karyotyping of chorionic villus samples (CVSs) is limited by cell culture and its low resolution. The objective of our study was to investigate the efficiency of molecular karyotyping technology for genetic diagnosis of early missed abortion tissues. Chromosome analysis of 1191 abortion CVSs in early pregnancy was conducted from August 2016 to June 2021; 463 cases were conducted via copy-number variations sequencing (CNV-seq)/quantitative fluorescent-polymerase chain reaction (QF-PCR) and 728 cases were conducted using SNP array. Clinically significant CNVs of CVSs were identified to clarify the cause of miscarriage and to guide the couples’ subsequent pregnancies. Among these, 31 cases with significant maternal cell contamination were removed from the study. Among the remaining 1160 samples, 751 cases (64.7%) with genetic abnormalities were identified, of which, 531 (45.8%) were single aneuploidies, 31 (2.7%) were multiple aneuploidies, 50 (4.3%) were polyploidies, 54 (4.7%) were partial aneuploidies, 77 (6.6%) had submicroscopic CNVs (including 25 with clinically significant CNVs and 52 had variants of uncertain significance), and 8 cases (0.7%) were uniparental disomies. Our study suggests that both SNP array and CNV-seq/QF-PCR are reliable, robust, and high-resolution technologies for genetic diagnosis of miscarriage.