bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chmp1A regulates pancreatic cancer cell growth via SIM-medicated SUMOylation and UIM-mediated ubiquitination, opposed through NLS-mediated signaling activities
Authors: Park Maiyon , Cai Mei , Thacker Austin Tyler , Roberts Carson , Qin Tiantian , Sakamoto Devin 
Published: December 2023

Abstract
Background Chmp1A is a member of ESCRT-III family that functions in the lysosomal degradation of proteins via the formation of MVBs at the final stage of lysosomal degradation. ESCRT-0, -I, and –II were shown to contain UIM for interaction with ubiquitinated cargo proteins destined for MVBs. However, Chmp1A of ESCRT-III has not previously been reported to contain UIM. The purpose of this study is to examine if Chmp1A contains motifs such as UIM through which it is modified and to examine the effect of these modifications on pancreatic cancer cell growth.Methods Protein database search was employed to investigate whether Chmp1A contains UIM or similar motif. Immunoprecipitation assays were used for examining posttranslational modifications, WB for protein expression, microscopy for subcellular localization, MTT assays for proliferation, transfection method for overexpression of various Chmp1A constructs in pancreatic cancer cells, and in vitro Ubiquitination assays for ubiquitination of rhChmp1A.Results We found that Chmp1A contains putative SIMs and a UIM. We observed that Chmp1A is slightly SUMOylated in normal pancreatic epithelial cells, which increases as pancreatic cancer progresses towards malignancy. In pancreatic cancer cells, full-length Chmp1A protein is significantly multi-SUMOylated but slightly mono-ubiquitinated. The NLS-deleted Chmp1A eliminated the PTMs by Sumo and ubiquitin, which were verified by SIM and UIM mutants. When overexpressed, Chmp1A was detected at the nucleus and the cytoplasm of which overlaps with late endosomal protein Lamp1. However, both mutants had no expression in the nucleus. SIM mutant showed spotty cytoplasm expression that overlaps with Lamp1. UIM mutant exhibited uniform cytoplasmic expression with little overlap with Lamp1. Both mutants of Chmp1A inhibited cell growth significantly, demonstrating that PTMs of Chmp1A are required for cancer cell growth.Conclusion We identified SIMs and a UIM of Chmp1A and verified its PTM by Sumo and ubiquitin. Based on our data, we conclude that SUMOylation and/or ubiquitination is essential for the nuclear signaling mediated cancer inhibitory functions of Chmp1A. We also conclude that the excess PTM of Chmp1A by Sumo and/or ubiquitin could lead to cancer promotion either by inhibiting tumor suppressors such as ATM or p53 or by activating oncogenic activities.
Nomogram for assistant diagnosing acute suppurative cholangitis: a case-control study
Authors: He Yu-qi , Han Wang , Zhao Yi-hang , Lv Guan-ting , Liu Zi-jun 
Published: December 2023

Abstract

Background:
Acute suppurative cholangitis (ASC) lacks sensitive and specific preoperative diagnostic criteria. This study aimed to investigate the relationship between the grading system of acute cholangitis (AC) according to the Tokyo Guidelines 2018 (TG18) and the diagnosis of acute suppurative cholangitis (ASC), searching for independent risk factors of ASC and develop a nomogram to discriminate ASC from acute nonsuppurative cholangitis (ANSC) accurately.
Methods:
After applying the inclusion and exclusion criteria, 401 patients with acute cholangitis (AC) were retrospectively analyzed at Nanjing First Hospital between January 2015 and July 2023. Based on the presence of suppurative bile, the patients were divided into two groups. SPSS version 27.0 and R studio software were used to analyze data obtained from medical records.
Results:
Among the 401 patients, 102 had suppurative bile (the ASC group; AC grade I: 40 [39.2%], AC grade II: 27 [26.5%], AC grade III: 35 [34.3%]), whereas 299 did not have (the ANSC group; AC grade I: 157 [52.5%], AC grade II: 92 [30.8%], AC grade III: 50 [16.7%]). Multivariate logistic regression analysis identified concurrent cholecystitis, CRP, PCT, TBA, and bile duct diameter as independent risk factors for suppurative bile, and all of these factors were included in the nomogram. The calibration curve exhibited consistency between the nomogram and the actual observation, and the area under the curve was 0.875 (95% confidence interval: 0.835–0.915), sensitivity was 86.6%, and specificity was 75.5%.
Conclusion:
The nomogram demonstrated excellent predictive performance in assisting in diagnosing ASC.
Chemotherapy-induced febrile neutropenia (FN) in the US: healthcare resource utilization (HCRU) and costs
Authors: Flanigan Jeanine A. , Yasuda Marie , Chen Chi-Chang , Li Edward C. 
Published: December 2023

Abstract
Purpose
Febrile neutropenia (FN) is a known side effect of chemotherapy, often requiring hospitalization. Economic burden increases with an FN episode and estimates of cost per episode should be updated from real-world data.
Methods
A retrospective claims analysis of FN episodes in patients with non-myeloid malignancies from 2014 to 2021 was performed in IQVIA PharMetrics® Plus database. FN episodes were defined as having same-day claims for neutropenia and fever or infection, plus antibiotic in outpatient settings, following a claim for chemotherapy; index date was defined as the first claim for neutropenia/fever/infection. Patients receiving bone marrow/stem cell transplant and CAR-T therapy were excluded, as were select hematologic malignancies or COVID-19. Healthcare utilization and costs were evaluated and described overall, by episode type (w/wo hospitalization), index year, malignancy type, NCI comorbidity score, and age group.
Results
7,033 FN episodes were identified from 6,825 patients. Most episodes had a hospitalization (91.2%) and 86% of patients had ≥ 1 risk factor for FN. Overall, FN episodes had a mean (SD) FN-related cost of $25,176 ($39,943). Episodes with hospitalization had higher average FN-related costs versus those without hospitalization ($26,868 vs $7,738), and costs increased with comorbidity score (NCI = 0: $23,095; NCI > 0–2: $26,084; NCI ≥ 2: $26,851).
Conclusions
FN continues to be associated with significant economic burden, and varied by cancer type, comorbidity burden, and age. In this analysis, most FN episodes were not preceded by GCSF prophylaxis. The results of this study highlight the opportunity to utilize GCSF in appropriate oncology scenarios.
Time required for psychological testing of dementia is useful for predicting asymptomatic brain lesions: validation using CADi2
Authors: Iwasa Kenichi , Takamura Masahiro , Kuroda Yoko , Asayama Kosuke , Aritake Shun , Abe Satoshi , Nagai Atsushi 
Published: December 2023

Abstract
Cognitive test duration is related to brain lesions. However, few studies have addressed automated timing. CADi2 is a cognitive evaluation software. This study aimed to evaluate the use of automatically calculating the time required to answer questions in a 10-item in CADi2, in addition to the questionnaire score, to assess cognitive function and estimate asymptomatic brain lesions. Among 556 patients who underwent a brain scan at the Shimane Institute of Health Science between March 28, 2019 and March 24, 2021 and interacted with CADi2, we enrolled 429 patients aged ≥ 50 years with paraventricular and deep subcortical white matter hyperintensity scores of ≥ 2 as per the Fazekas classification, microbleeds, and asymptomatic brain lesions. CADi2 total time was more accurate at predicting the presence of a silent brain infarction (SBI) than the Mini-Mental State Examination Particularly, the time required to answer questions 2, 7, and 8 was related to the presence of SBI. The odds ratio of having SBI was 3.75 (2.00–7.05) when using the summed time to answer these three questions. The time required for cognitive testing using CADi2 is useful for predicting the presence of asymptomatic lesions and could be used as a non-invasive, prehospital stroke risk screening tool.
Non-malignant pathological results from CT-guided biopsy for pulmonary nodules: A predictive model for identifying false-negative results
Authors: Wang Xu-Zhou , Wang Jing-Ya , Meng Tao , Shi Yi-Bing , Sun Jin-Jun 
Published: December 2023

Abstract
Background: Computed tomography (CT)-guided biopsy (CTB) procedures are commonly used to aid in the diagnosis of pulmonary nodules (PNs). When CTB findings indicate a non-malignant lesion, however, it is vital that false-negative results are accurately identified. Accordingly, the present study was designed with the goal of identifying relevant predictors for the construction of a model capable of predicting false-negative cases among patients undergoing CTB for PNs who receive non-malignant results.
Materials and Methods: Consecutive patients from two centers who received CTB-based non-malignant pathological results when undergoing evaluation for PNs from January 2016 to December 2020 were retrospectively evaluated. A training cohort was used to identify factors that were predictive of false negative results, enabling the establishment of a predictive model. The remaining patients were used to establish a testing cohort that served to validate predictive model accuracy.
Results: The training cohort enrolled 102 patients with PNs exhibiting CTB-based non-malignant pathological findings, each of whom underwent CTB for a single nodule. Of these patients, 85 and 17 respectively exhibited true negative and false negative PNs. Through univariate and multivariate analyses, higher standardized maximum uptake values (SUVmax, P = 0.001) and CTB-based findings of suspected malignant cells (P = 0.043) were identified as being predictive of false negative results. These two predictors were then combined to establish a predictive model. The area under the receiver operating characteristic curve (AUC) for this model was 0.945, with corresponding sensitivity and specificity values of 88.2% and 87.1%. The testing cohort included 62 patients, each of whom had a single PN. When the developed model was used to evaluate this testing cohort, this yielded an AUC value of 0.851.
Conclusions: The predictive model developed herein exhibited good diagnostic utility when identifying false-negative CTB-based non-malignant pathological results among patients with PNs.
Clinical characteristics of severe influenza and molecular epidemiology of influenza viruses
Authors: Bo Yan , Miao Chuhan , Li Beilei , Yang Yumeng , He Jianxun 
Published: December 2023

Abstract
Background
The protocol aims to investigate the clinical features of severe cases of influenza and the genetic diversity and transmission patterns of influenza viruses.
Methods
The study collects clinical data from patients with severe flu, including symptoms, disease progression, and outcomes. Additionally, molecular analysis will be conducted to identify and characterise the influenza viruses circulating in the population.
Discussion
The findings from this study will provide valuable insights into the clinical manifestations of severe influenza and the genetic variations of influenza viruses, which can contribute to the development of effective prevention and control strategies.
• Guiding significance for improving the understanding of recent influenza epidemic patterns and diagnosis and treatment capabilities.
• Provide practical biological evidence for influenza diagnosis and prevention.
Trial registration
retrospectively registered.
Strengthening initial teacher education programs to deliver confident, effective, classroom-ready graduates: A feasibility study
Authors: Riley Nicholas , Leahy Angus A. , Eather Narelle , Verswijveren Simone J.J.M. , Lander Natalie 
Published: December 2023

Abstract
Purpose TransformEd is an evidence-based program that targets initial teacher education to equip future teachers with innovative strategies that increase the health, wellbeing and education outcomes of school children. Feasibility, effectiveness and implementation research demonstrate that TransformEd significantly enhances pre-service teachers’ confidence and competence to deliver such strategies. However, to date, the program has only been tested in undergraduate teaching degrees. It is important to gather more evidence for the feasibility and efficacy of the program at all levels of initial teacher education, including at postgraduate level.
Methods A pilot feasibility study was conducted to examine the feasibility of embedding TransformEd in a Master of Teaching degree at one Australian university. Preliminary efficacy was measured by pre- and post-program surveys completed by Master of Teaching pre-service teachers who received the 12-week TransformEd program. Implementation was measured by adherence checklists completed by lecturers who delivered TransformEd. Post-program focus groups and interviews with senior academics and lecturers, respectively, explored perceptions of all eight domains of feasibility.
Results Survey results demonstrated that the TransformEd program was positively received by pre-service teachers (n=21), resulting in improvements in several measures of teaching confidence and competence in regard to delivering strategies to improve school children’s health, wellbeing and learning. The TransformEd strategies were well adopted by lecturers (n=3) and implementation increased over time. Both lecturers and senior academics (n=2) had positive perceptions of the program, highlighting its feasibility in postgraduate initial teacher education.
Conclusion This pilot study provides support for the feasibility TransformEd program in the context of postgraduate initial teacher education. The findings have direct implications for the progression of TransformEd from pilot feasibility to a future definitive trial in postgraduate initial teacher education, which may provide a population –level impact on the health, wellbeing and education outcomes of all Australian children.
A study on the causes of chronic obstructive pulmonary disease
Authors: Jebbeh Omar Al , Khalil Mohammed Abdulbasset , Ali Diaa Haj , Al-Bardan Hussam 
Published: December 2023

Abstract
Background: The importance of the research lies in that it sheds light on an important medical issue, which is the causes leading to chronic obstructive pulmonary disease, and shows the extent of its danger to the individual and its negative role in exacerbating the disease.
Materials and methods: A retrospective cohort study, was conducted in Damascus Hospital in the Syrian Arab Republic in the city of Damascus from April 2020 to November 2023. The study included patients with chronic obstructive pulmonary disease (COPD)
Results: a sample of 200 patients with COPD who participated in the study, the largest percentage were from the group of 61 years and over with a percentage of 68%, and the largest percentage were from males with 78%. 98% of the sample were smokers. 21% suffered from asthma, 20% used firewood, and the dominant symptom was dyspnea, at a rate of 91%, followed by cough, at a rate of 88%, and 94.3 % of those who had a cough were productive, not dry. 51.9% had mucous sputum.
48.3% of patients had dyspnea of the fourth degree, and 90% of patients had performed chest X-ray.
Conclusion: It is necessary to give special importance to the causes leading to chronic obstructive pulmonary disease in terms of management and limiting its spread, as it effectively contributes to reducing the severity of the disease and preserving the organ.
Stress and Coping Strategies among Medical Students in Dubai, United Arab
Emirates, in 2020: A Cross-Sectional Study
Authors: Boushehri Yas K. , Jeyaseelan Lakshmanan , Sultan Meshal A. 
Published: December 2023

Background::
Studies conducted worldwide have found that medical students have higher levels of stress than students in other fields. Chronic stress can result in
increased rates of fatigue, depression, and impairment in function. Although this phenomenon has been studied globally, there has been limited
attention in our region. Therefore, the purpose of this study is to assess stress levels in medical students in Dubai and evaluate their coping
strategies to highlight the importance of shedding light on the psychological well-being of this population.

Methods::
A cross-sectional design was utilized for this study. The target population consisted of all medical students enrolled at a local university for the
2020-2021 academic year. The participants completed a survey that collected information on stress and coping strategies. The Perceived Stress
Scale (PSS-10) and the Brief COPE inventory were utilized to gather the data.

Results::
The total of 97 individuals completed the study questionnaire. The rates of high stress levels according to the Perceived Stress Scale (PSS-10) were
higher among medical students in the first to the third year (43.6%), when compared to medical students in the fourth and fifth years (7.7%).
Results of the Brief COPE inventory indicated that the average score for a Problem Focused coping style among medical students fell in the
medium to high range (22 out of 32).

Conclusion::
Levels of stress are elevated among medical students. Coping responses reflect the individual’s psychological adjustment and well-being. Our
study sample revealed a medium to high rate of engagement in healthy coping strategies. Future research that evaluates the psychological impact of
stress in-depth and explores effective strategies to enhance coping is highly warranted.
Teologiczna ocena moralna cywilizacji naukowo-technicznej w świetle nauki Jana Pawła II
Authors: Rel Małgorzata 
Published: December 2023

„Człowiek dzisiejszy zdaje się być stale zagrożony przez to, co jest jego własnym wytworem, co jest wynikiem pracy jego rąk, a zarazem – i bardziej jeszcze – pracy jego umysłu, dążeń jego woli. Owoce tej wielorakiej działalności człowieka (…), w jakimś pochodnym i pośrednim zakresie skutków – skierowują się przeciw człowiekowi.” (Jan Paweł II). Papieski postulat domagający się proporcjonalnego do rozwoju cywilizacji naukowo – technicznej, rozwoju moralności i etyki, wskazuje na możliwe postawy współczesnego człowieka inspirowane antropologią chrześcijańską. Na przykładzie encyklik Redemptor Hominis i Fides et Ratio Jana Pawła II, chciałabym przedstawić rozważania dotyczące relacji człowieka wobec rozwoju cywilizacji naukowo – technicznej oraz zastanowić się: „czy człowiek jako człowiek, w kontekście tego postępu staje się lepszy, (…) bardziej gotowy świadczyć i nieść pomoc wszystkim?”.
Respons Pertumbuhan dan Hasil Produksi Pegagan pada Pemberian Pupuk Organik Cair dan Pupuk Kandang Sapi
Authors: Firgiyanto Refa , Antika Hermin , Elfina Vera , Kusparwanti Tri Rini , Rohman Hanif Fatur , Siswadi Edi , Sukri M. Zayin 
Published: December 2023

Tanaman pegagan merupakan tanaman yang memiliki banyak manfaat untuk kesehatan sebagai tanaman obat, sehingga diperlukan upaya untuk meningkatkan produktivitas tanaman. Salah satu upaya yang dilakukan antara lain perbaikan teknik budidaya melalui penambahan pupuk organik. Tujuan penelitian yaitu untuk mengetahui pengaruh tunggal dan interaksi pemberian konsentrasi pupuk organik cair bonggol pisang dan dosis pupuk kandang sapi. Penelitian dilakukan di Screen house Laboratorium Tanaman, Politeknik Negeri Jember (89 mdpl). Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) Faktorial. Faktor pertama adalah konsentrasi pupuk organik cair 0, 50, dan 100 ml/l dan faktor kedua yaitu dosis pupuk kandang 0, 200, dan 400 g/polybag. Variabel pengamatan pada penelitian ini antara lain jumlah daun, jumlah stolon, jumlah anakan, panjang stolon terpanjang, panjang tangkai terpanjang, berat basah tanaman, berat kering tanaman, berat akar tanaman, kandungan klorofil tanaman, dan kandungan flavonoid tanaman pegagan. Hasil penelitian menunjukkan bahwa faktor tunggal pemberian POC belum berpengaruh secara nyata pada seluruh variabel pengamatan sedangkan pemberian pupuk kandang sapi mampu meningkatkan secara nyata panjang tangkai tanaman pegagan dengan dosis terbaik pada perlakuan 200 dan 400 g/polybag. Interaksi pemberian konsentrasi POC dan pupuk kandang berpengaruh nyata pada variabel jumlah daun 2 dan 4 MST, panjang stolon, dan berat akar tanaman. Pemberian terbaik didapatkan pada perlakuan pemberian POC 100 ml/l dan dosis pupuk kandang sapi 200 g/polybag.
Analisis Pektin dan Vitamin C untuk Evaluasi Sifat Tahan Simpan pada Generasi F2 Buah Cabai LB
Authors: Izi Muhammad Nur , Chairunisa Chairunisa , Wahyuni Wahyuni , Herliana Lina , Seftiono Hermawan 
Published: December 2023

Cabai (Capsicum annuum L.) merupakan salah satu komoditas sayuran terpenting dan strategis di Indonesia. Sebagai salah satu komoditas terpenting, cabai memiliki masa simpan pascapanen yang relatif singkat yang seringkali menjadi penyebab kerugian ekonomi. Ketahanan daya simpan cabai dapat ditingkatkan melalui upaya persilangan. Persilangan antara dua varietas cabai, Laris (daya hasil tinggi) dan SSP (tahan simpan) telah menghasilkan generasi kedua (F2) cabai LB. Buah cabai F2 LB matang merah telah dikelompokkan berdasarkan daya simpannya, dilihat dari susut bobot buah pascapanen. Pada studi ini, dilakukan pengukuran kandungan pektin dan vitamin C pada buah cabai F2 LB matang merah. Pektin dan vitamin C merupakan bagian komponen struktural dan antioksidan penjaga keutuhan dinding sel yang penting untuk ketahanan simpan buah. Tujuan penelitian adalah menyelidiki korelasi antara kandungan pektin atau vitamin C dengan sifat ketahanan simpan buah cabai. Analisis juga dilakukan pada buah cabai tetua Laris dan SSP sebagai kontrol. Pengukuran pektin dilakukan menggunakan Pectin Identification Kit (Megazyme) dengan dua protokol tambahan. Kandungan pektin dan vitamin C diukur secara semi kuantitatif menggunakan spektrofotometer. Hasil menunjukkan bahwa kandungan pektin dan vitamin C pada buah cabai F2 LB tidak berbeda signifikan antara yang tahan simpan dengan yang tidak tahan simpan, maupun dengan kedua tetuanya. Hasil ini mengindikasikan bahwa variasi ketahanan simpan yang teramati pada sampel buah cabai tidak bergantung pada kandungan pektin maupun vitamin C. Kami menduga bahwa aktivitas enzimatik, terutama yang terlibat dalam proses degradasi komponen dinding sel, lebih berperan dalam menentukan sifat ketahanan simpan cabai.
Pemberdayaan Masyarakat Dalam Pengelolan Sampah Rumah Tangga di Desa Peliatan Ubud
Authors: Cahyawati Putu Nita , Santosa Agus , Lestarini Asri , Aryastuti Anak Agung Sri Agung 
Published: December 2023

Ubud merupakan salah satu destinasi wisata yang terkenal di Bali. Desa Peliatan merupakan salah satu desa yang berlokasi di Ubud. Sampah merupakan salah satu dampak negatif akibat perkembangan pariwisata desa ini. Mitra pada kegiatan pengabdian ini adalah warga Desa Peliatan. Metode pelaksanaan kegiatan terdiri dari 3 tahapan yaitu persiapan, pelaksanaan, dan monitoring. Indikator keberhasilan program yaitu partisipasi mitra, peningkatan pengetahuan mitra, dan pemberian bantuan pada mitra. Mitra yang hadir pada kegiatan ini berjumlah 20 orang. Pemberian materi dilaksanakan sebanyak 1 kali pertemuan. Pada awal kegiatan dilakukan pretest untuk menilai pengetahuan awal mitra. Hasil pretest menunjukkan rerata nilai pretest sebesar 5,05. Pada akhir kegiatan dilakukan postest. Hasil penilaian postest ditemukan rerata nilai postest sebesar 8,1. Bantuan tim pengabdian diterima secara simbolis oleh Kepala Desa. Kegiatan ini mampu meningkatkan pengetahuan mitra dari rerata nilai 5,05 menjadi 8,1. Seluruh indikator keberhasilan program telah terpenuhi sehingga dapat disimpulkan bahwa kegiatan telah terlaksana dengan baik.