bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of lung metastasis on the treatment and prognosis of patients with gestational trophoblastic neoplasia: A systematic review and meta‐analysis
Authors: Zhang Taohong , Guo Ying , He Xinyi , Hou Meng , Wang Lisha , An Ruifang , Gao Li 
Published: January 2024

AbstractIntroductionGestational trophoblastic neoplasia (GTN) is a highly invasive tumor, mainly spreading to the lungs. However, lung metastasis in GTN is usually not considered as an adverse prognostic factor. Therefore, the aim of this study was to summarize the results of previous studies and evaluate the effects of lung metastasis on the treatment and prognosis of GTN.Material and methodsThe study was prospectively registered in PROSPERO (CRD42023372371). Electronic databases including PubMed, Embase, the Cochrane Library, Chinese National Knowledge Infrastructure, Wanfang, and China Biomedical Literature Database were used for a systematical search of relevant studies published up to November 21, 2022. The observational studies reporting the clinical outcomes of GTN patients with and without lung metastasis were selected. The incidences of resistance, relapse, and mortality of GTN patients were extracted and successively grouped based on the presence of lung metastasis. The pooled relative risks (RRs) and 95% confidence interval (95% CI) of the eligible studies were calculated. The qualities of included studies were assessed with the Newcastle‐Ottawa Scale and the certainty of evidence was graded based on the GRADE. The meta‐analysis was performed using Stata 12.0 and GradePro software.ResultsFive publications with 3629 GTN patients were included. The meta‐analysis revealed that the GTN with lung metastasis was strongly correlated with first‐line chemoresistance (pooled RR = 1.40, 95% CI: 1.22 to 1.61, p < 0.001), recurrence (pooled RR = 3.03, 95% CI: 1.21 to 7.62, p = 0.018), and disease‐specific death (pooled RR = 22.11, 95% CI: 3.37 to 145.08, p = 0.001). Ethnicity was also an important factor and Caucasian GTN patients with lung metastasis showed a higher risk of recurrence as revealed by the subgroup analysis (pooled RR = 5.10, 95% CI: 2.38 to 10.94, p < 0.001).ConclusionsGTN patients with lung metastasis exhibited a higher risk of chemoresistance, relapse, and disease‐specific death. Patients with lung metastasis among the Caucasian population had a higher risk of recurrence than Asian populations. Therefore, the presence of lung metastases might be considered as a high‐risk factor for prognosis of GTN and deserves more attention in the choice of first‐line chemotherapy regimens and follow‐up.
“You don't know what you don't know”: A qualitative study of informational needs of patients, family members, and living donors to inform transplant system metrics
Authors: Hart Allyson , Schaffhausen Cory R. , McKinney Warren T. , Gonzales Kristina , Perugini Julia , Snyder Jon J. , Ladin Keren 
Published: January 2024

AbstractIntroductionInformational needs and potential use of transplant metrics, especially among patients, remain understudied and a critical component of the transplant community's commitment to patient‐centered care. We sought to understand the perspectives and needs of patients, family members/caregivers, living donors, and deceased donor family members.MethodsWe examined decision‐making experiences and perspectives on the needs of these stakeholder groups for data about the national transplant system among 58 participants of 14 focus groups and 6 interviews.ResultsThree major themes emerged: 1) informational priorities and unmet needs (transplantation system processes, long‐term outcomes data, prelisting data, patient‐centered outcomes, and ability to compare centers and regions); 2) challenges obtaining relevant and trustworthy information (patient burden and effort, challenges with medical jargon, and difficulty finding trustworthy information); and 3) burden of facing the unknown (stress and anxiety leading to difficulty processing information, challenges facing the transplant journey when you “don't know what you don't know”).ConclusionPatient, family member, and living donor participation in shared decision‐making has been limited by inadequate access to patient‐centered information. New metrics and patient‐facing data presentations should address these content gaps using best practices to improve understanding and support shared decision‐making.
Macrophages enhance regeneration of lateral line neuromast derived from interneuromast cells through TGF‐β in zebrafish
Authors: Hsu Wei‐Lin , Lin Yu‐Chi , Lin Meng‐Ju , Wang Yi‐Wen , Lee Shyh‐Jye 
Published: January 2024

AbstractMacrophages play a pivotal role in the response to injury, contributing significantly to the repair and regrowth of damaged tissues. The external lateral line system in aquatic organisms offers a practical model for studying regeneration, featuring interneuromast cells connecting sensory neuromasts. Under normal conditions, these cells remain dormant, but their transformation into neuromasts occurs when overcoming inhibitory signals from Schwann cells and posterior lateral line nerves. The mechanism enabling interneuromast cells to evade inhibition by Schwann cells remains unclear. Previous observations suggest that macrophages physically interact with neuromasts, nerves, and Schwann cells during regeneration. This interaction leads to the regeneration of neuromasts in a subset of zebrafish with ablated neuromasts. To explore whether macrophages achieve this effect through secreted cytokines, we conducted experiments involving tail amputation in zebrafish larvae and tested the impact of cytokine inhibitors on neuromast regeneration. Most injured larvae remarkably regenerated a neuromast within 4 days post‐amputation. Intriguingly, removal of macrophages and inhibition of the anti‐inflammatory cytokine transforming growth factor‐beta (TGF‐β) significantly delayed neuromast regeneration. Conversely, inhibition of the pro‐inflammatory cytokines interleukin‐6 (IL‐6) and tumor necrosis factor‐alpha (TNF‐α) had minor effects on the regeneration process. This study provides insights into how macrophages activate interneuromast cells, elucidating the pathways underlying neuromast regeneration.
Dyadic effects of stigma on quality of life in people with schizophrenia and their family caregivers: Mediating role of patients' perception of caregivers' expressed emotion
Authors: Peng Yanan , Xu Ronghua , Li Yan , Li Ling , Song Lanjun , Xi Juzhe 
Published: January 2024

AbstractSchizophrenia, as a stressful diagnosis, profoundly impacts the whole family, especially people with schizophrenia and their caregivers. This study tested the potential mediating role of expressed emotion in the association between mental health stigma and quality of life in caregiver‐patient dyads. Using a 2‐wave longitudinal design with a 6‐month interval between assessments, 161 dyads of patients with schizophrenia and their family caregivers (one patient and one caregiver) completed measures of mental health stigma, expressed emotion, and quality of life. The results showed that patients' self‐stigma had no significant actor or partner effect on expressed emotion or quality of life. In contrast, caregivers' stigmatizing attitudes toward patients had a significant partner effect on patients' perception of caregivers' expressed emotion and quality of life. The mediating effect of patients' perception of caregivers' expressed emotion in the association between caregivers' stigmatizing ideas toward patients and patients' quality of life was significant. By focusing on the interdependence of patients and their caregivers, this study highlights the role of caregivers' stigmatizing attitudes toward patients and patients' perception of caregivers' expressed emotion on patients' quality of life. Psychoeducation and interventions should not only aim to reduce the self‐stigma of people with schizophrenia but also their caregivers' stigmatizing ideas toward patients. Family interventions targeted at reducing the EE level of caregivers and patients' perception of caregivers' EE would also benefit the adaptation and quality of life of people with schizophrenia and their caregivers.
Patient‐reported outcome measures in adult HIV care: A rapid scoping review of targeted outcomes and instruments used
Authors: Engler Kim , Avallone Francesco , Cadri Abdul , Lebouché Bertrand 
Published: January 2024

AbstractObjectiveThere is international interest in the integration of patient‐reported outcome measures (PROMs) into routine HIV care, but little work has synthesized the content of published initiatives. We conducted a rapid scoping review primarily to identify their selected patient‐reported outcomes and respective instruments.MethodsFour databases were searched on 4 May 2022 (Medline, Embase, CINAHL and PsychINFO) for relevant English language documents published from 2005 onwards. Dual review of at least 20% of records, full texts and data extraction was performed. Outcomes and instruments were classified with an adapted 14‐domain taxonomy. Instruments with evidence of validation were described.ResultsOf 13 062 records generated for review, we retained a final sample of 94 documents, referring to 60 distinct initiatives led mostly in the USA (n = 29; 48% of initiatives), Europe (n = 16; 27%) and Africa (n = 9; 15%). The measured patient‐reported outcome domains were: mental health (n = 42; 70%), substance use (n = 23; 38%), self‐management (n = 16; 27%), symptoms (n = 12; 20%), sexual/reproductive health (n = 12; 20%), physical health (n = 9; 15%), treatment (n= 8; 13%), cognition (n = 7; 12%), quality of life (n = 7; 12%), violence/abuse (n = 6; 10%), stigma (n = 6; 10%), socioeconomic issues (n = 5; 8%), social support (n = 3; 5%) and body/facial appearance (n = 1; 2%). Initiatives measured 2.6 outcome domains, on average (range = 1–11). In total, 62 distinct validated PROMs were identified, with 53 initiatives (88%) employing at least one (M = 2.2). Overwhelmingly, the most used instrument was any version of the Patient Health Questionnaire to measure symptoms of depression, employed by over a third (26; 43%) of initiatives.ConclusionPublished PROM initiatives in HIV care have spanned 19 countries and disproportionately target mental health and substance use.
Use of clinical biological tests of haemostasis to evaluate topical haemostatics
Authors: Nativel Fabien , Tollec Sophie , Sellal Kamel‐Olivier , Trossaërt Marc , Grimandi Gaël 
Published: January 2024

AbstractIntroductionIn addition to traditional means, topical haemostatics are currently used to avoid haemorrhage during surgery. Although they have been reported to be effective, there is a low level of proof of their clinical efficacy, which is at odds with their levels of use. This study used two methods to better understand their in vitro mechanism of action.MethodsTwo clinical biology assays were used to measure the action of topical haemostatics on primary and secondary haemostasis. Calibrated samples of collagen sponges and polypropylene non‐woven gauze were tested. Platelet aggregation was assessed using a multichannel aggregometer. A thrombin generation assay (TGA) was used with a fluorogenic readout. Tissue factor solutions were used to activate coagulation.ResultsIn terms of primary haemostasis, collagen sponges stimulated platelet aggregation, in particular between 2 and 5 min after incubation with platelet‐rich plasma and with no dose effect. In regard to coagulation, the kinetics of thrombin generation was enhanced. Polypropylene non‐woven gauze did not exhibit any effect on platelet aggregation, although it did have a weak effect on the kinetics of thrombin generation.ConclusionCollagen is well known to exert a haemostatic effect due to its action on platelet aggregation. By contrast, polypropylene non‐woven gauze has not been shown to have any effect on platelet aggregation other than a minor impact on thrombin generation. The results obtained with the devices tested are in agreement with the literature. Platelet aggregation biological assays and TGA measurements appear to be suitable for evaluation of these medical products.
Fabrication of vanadium telluride anchored on carbon nanotubes nanocomposite for overall water splitting
Authors: Bano Nigarish , Manzoor Sumaira , Sami Abdus , Shah Syed Imran Abbas , Junaid Ali , Rehman Muhammad Yousaf Ur , Alshgari Razan A. , Ehsan Muhammad Fahad , Ashiq Muhammad Naeem 
Published: January 2024

AbstractElectrocatalytic water splitting is an essential hydrogen production method for resolving present energy shortage and progress toward more efficient technologies. For this purpose, a versatile and cheap electrocatalysts are the main challenge along the way. In this report, we synthesized vanadium telluride and carbon nanotube (VTe–CNT)‐based nanocomposite via facile hydrothermal route. The VTe–CNTs are characterized by X‐ray diffraction analysis, scanning electron microscopy, energy‐dispersive spectroscopy, Fourier transform infrared spectroscopy, and Brunauer–Emmett–Teller. These characterizations depict nanosphere structures, morphology, and high surface area that maintains high porosity, which are essential for inclusive water‐splitting phenomena in 1.0 M solution of KOH. Additionally, the electrochemical performance of VTe–CNTs has shown best O2 evolution reactions activity with of onset potential of 1.42 V versus reversible hydrogen electrode and required 10 mA/cm2 of current density at 278 mV overpotential, which is excellent among other electrocatalysts, VTe (342 mV@10 mA/cm−2) and CNTs (365 mV@10mA/cm−2). Moreover, VTe–CNT exhibits remarkable stability for almost 20 h. It also requires a low onset potential of 0.05 V with a small Tafel slope of 47 mV/dec for H2 evolution reactions. Hence, this research might facilitate the easy transportation of electrons and open up the new era, serving as an excellent replacement for noble metal–derived materials.
X‐ray‐activated super long persistent ultraviolet A luminescence from Ca3(PO4)2: Ce3+
Authors: Wang Shuya , Sun Wenzhi , Zhao Tingting , Fu Chunqing , Fu Xiaoyan , Zhang Hongwu 
Published: January 2024

AbstractIn this paper, X‐ray‐activated super long persistent ultraviolet A luminescence from Ca3(PO4)2: Ce3+ has been observed. The crystal structure analysis has shown that the as‐prepared samples are pure β‐Ca3(PO4)2 phases with rhombohedral structure and R3c space group. The photoluminescent spectra of the samples all consist of a broad band in 340–400 nm UVA1 region, which is assigned to the 5d1–4f1 electronic transition of Ce3+ ions. More important, under the 3 min X‐ray irradiation, Ca3(PO4)2: Ce3+ show super long persistent luminescence in the UVA1 region, which can persist for more than 16 h. And its afterglow intensity after 16 h decay is still 4.10 times stronger of the back ground intensity. Furthermore, only 10 s X‐ray irradiation can induce excellent long persistent UVA luminescence of Ca3(PO4)2: 0.08Ce3+, which can persist for more than 5 h and afterglow emission intensity is about 3.03 times of the background intensity. The trap analysis has confirmed that the different X‐ray irradiation time do not create new traps and there is the same trap with 0.75 eV trap depth existing even under the extremely low X‐ray dose (10 s X‐ray irradiation). Furthermore, under 10 s X‐ray irradiation, the thermoluminescence (TL) intensity of Ca3(PO4)2: 0.08Ce3+ still reaches 0.32 times of the initial TL intensity even after 120 min, which thus leads to the super long persistent luminescence. Thus, the large amounts of trapped electrons induced by the high energy X‐ray irradiation are the principal cause for super long persistent UVA luminescence of Ca3(PO4)2: Ce3+.
Predictors of first pass isolation of the pulmonary veins in real world ablations: An analysis of 2671 patients from the REAL‐AF registry
Authors: Kreidieh Omar , Hunter Tina D. , Goyal Sandeep , Varley Allyson L. , Thorne Christopher , Osorio Jose , Silverstein Josh , Varosy Paul , Metzl Mark , Leyton‐Mange Jordan , Singh David , Rajendra Anil , Moretta Antonio , Zei Paul C. ,   Investigators of the REAL AF registry
Published: January 2024

AbstractIntroductionDuring atrial fibrillation ablation (AFA), achievement of first pass isolation (FPI) reflects effective lesion formation and predicts long‐term freedom from arrhythmia recurrence. We aim to determine the clinical and procedural predictors of pulmonary vein FPI.MethodsWe reviewed AFA procedures in a multicenter prospective registry of AFA (REAL‐AF). A multivariate ordinal logistic regression, weighted by inverse proceduralist volume, was used to determine predictors of FPI.ResultsA total of 2671 patients were included with 1806 achieving FPI in both vein sides, 702 achieving FPI in one, and 163 having no FPI. Individually, age, left atrial (LA) scar, higher power usage (50 W), greater posterior contact force, ablation index >350 posteriorly, Vizigo™ sheath utilization, nonstandard ventilation, and high operator volume (>6 monthly cases) were all related to improved odds of FPI. Conversely sleep apnea, elevated body mass index (BMI), diabetes mellitus, LA enlargement, antiarrhythmic drug use, and center's higher fluoroscopy use were related to reduced odds of FPI. Multivariate analysis showed that BMI > 30 (OR 0.78 [0.64–0.96]) and LA volume (OR per mL increase = 1.00 [0.99–1.00]) predicted lower odds of achieving FPI, whereas significant left atrial scarring (>20%) was related to higher rates of FPI. Procedurally, the use of high power (50 W) (OR 1.32 [1.05–1.65]), increasing force posteriorly (OR 2.03 [1.19–3.46]), and nonstandard ventilation (OR 1.26 [1.00–1.59]) predicted higher FPI rates. At a site level, high procedural volume (OR 1.89 [1.48–2.41]) and low fluoroscopy centers (OR 0.72 [0.61–0.84]) had higher rates of FPI.ConclusionFPI rates are affected by operator experience, patient comorbidities, and procedural strategies. These factors may be postulated to impact acute lesion formation.
CLOSE protocol versus lower ablation index value for paroxysmal atrial fibrillation: A randomized noninferior clinical trial
Authors: Zeng Lijun , Chen Shi , Zeng Rui , Hu Hongde , Yang Qing , Cui Kaijun , Chen Qingyong , Fu Hua , Pu Xiaobo 
Published: January 2024

AbstractIntroductionThe optimized ablation index (AI) value for catheter ablation of atrial fibrillation (AF) remains to be defined. We aimed to compare the efficacy and safety of CLOSE protocol and lower AI protocol in paroxysmal AF.Methods and ResultsPatients with symptomatic, drug‐resistant paroxysmal AF for first ablation were prospectively enrolled from September 2020 to January 2022. The patients were randomly divided into CLOSE group (AI ≥ 550 for anterior/roof segments and ≥400 for posterior/inferior segments) and lower AI group (AI ≥ 450 for anterior/roof segments and ≥350 for posterior/inferior segments). First‐pass isolation, acute pulmonary vein (PV) reconnections, 1‐year arrhythmia recurrence, and major complications were assessed. Of the 270 enrolled patients, 238 completed 1‐year follow‐up (118 in CLOSE group and 120 in lower AI group). First‐pass isolation in left PVs was higher in CLOSE group (71.2% vs. 53.3%, p = .005). Acute PV reconnections were comparable between groups (9.3% vs. 14.2%, p = .246). At 1 year, 86.4% in CLOSE group versus 81.7% in lower AI group were free from atrial arrhythmia (log rank p = .334). The proportion difference was −4.8% (95% CI: −14.1% to 4.6%), and p = .475 for noninferiority. Stroke occurred in four patients of lower AI group, and no cardiac tamponade, atrioesophageal fistula, major bleeding or death occurred post procedure.ConclusionFor patients with paroxysmal AF and treated by AI‐guided PV ablation, lower AI is not noninferior to CLOSE protocol.
Development and characterization of brain cell line from Trachinotus blochii and its application in virological and gene expression studies
Authors: Nafeez Ahmed A. , Mithra S. , Suryakodi S. , Taju G. , Abdul Wazith M. J. , Kanimozhi K. , Rajkumar V. , Badhusha A. , Abdul Majeed S. , Sahul Hameed A. S. 
Published: January 2024

AbstractA permanent cell line, SPB (Snubnose pompano brain) was established from Trachinotus blochii by the explant culture method. It has been sub‐cultured more than 75 passages and showed optimal growth at 28°C using L‐15 medium supplemented with 15% to 20% FBS. The SPB cells were cryopreserved at different passage levels for various applications. SPB cells were composed of fibroblastic and epithelial‐like cells. The SPB cells were tested for mycoplasma contamination which was found to be negative. The origin of the SPB cell line from T. blochii was confirmed by amplification of the mitochondrial cytochrome oxidase I (COI) gene. The transfection efficiency of SPB cell line is 15% assessed by expression of green fluorescent protein using pEGFP‐N1 plasmid. In addition, two CMV promotor plasmids pFNCPE42‐DNA and pcDNAVP28 were transfected to SPB cells and it shows high expression levels of FNCP of fish nodavirus and VP28 protein of white spot syndrome virus by immunostaining. The SPB cells showed susceptibility to SJNNV and the infection was confirmed by RT‐PCR, Western blot, ELISA, TCID50 and RT‐qPCR. Experimental infection was carried out in T. blochii using SJNNV propagated in SPB cell line and found 100% mortality with clinical signs. The infection was confirmed by RT‐PCR. The SPB cell line can be used for propagation of fish viral pathogens and production of the recombinant proteins.
MdbHLH162 connects the gibberellin and jasmonic acid signals to regulate anthocyanin biosynthesis in apple
Authors: An Jian‐Ping , Xu Rui‐Rui , Wang Xiao‐Na , Zhang Xiao‐Wei , You Chun‐Xiang , Han Yuepeng 
Published: January 2024

ABSTRACTAnthocyanins are secondary metabolites induced by environmental stimuli and developmental signals. The positive regulators of anthocyanin biosynthesis have been reported, whereas the anthocyanin repressors have been neglected. Although the signal transduction pathways of gibberellin (GA) and jasmonic acid (JA) and their regulation of anthocyanin biosynthesis have been investigated, the cross‐talk between GA and JA and the antagonistic mechanism of regulating anthocyanin biosynthesis remain to be investigated. In this study, we identified the anthocyanin repressor MdbHLH162 in apple and revealed its molecular mechanism of regulating anthocyanin biosynthesis by integrating the GA and JA signals. MdbHLH162 exerted passive repression by interacting with MdbHLH3 and MdbHLH33, which are two recognized positive regulators of anthocyanin biosynthesis. MdbHLH162 negatively regulated anthocyanin biosynthesis by disrupting the formation of the anthocyanin‐activated MdMYB1‐MdbHLH3/33 complexes and weakening transcriptional activation of the anthocyanin biosynthetic genes MdDFR and MdUF3GT by MdbHLH3 and MdbHLH33. The GA repressor MdRGL2a antagonized MdbHLH162‐mediated inhibition of anthocyanins by sequestering MdbHLH162 from the MdbHLH162‐MdbHLH3/33 complex. The JA repressors MdJAZ1 and MdJAZ2 interfered with the antagonistic regulation of MdbHLH162 by MdRGL2a by titrating the formation of the MdRGL2a‐MdbHLH162 complex. Our findings reveal that MdbHLH162 integrates the GA and JA signals to negatively regulate anthocyanin biosynthesis. This study provides new information for discovering more anthocyanin biosynthesis repressors and explores the cross‐talk between hormone signals.