bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ten polymorphic autosomal microsatellite loci for the Eider duck Somateria mollissima and their cross‐species applicability among waterfowl species (Anatidae)
Authors: Paulus K. B. , Tiedemann R. 
Published: May 2003

AbstractWe describe the isolation and characterization of the first microsatellite loci specifically developed for the Common Eider duck, Somateria mollissima. Our 10 loci show Mendelian inheritance and no linkage disequilibrium between any pair of loci. In the Eider duck, we observed between two and 16 alleles per locus, an expected heterozygosity between 0.31 and 0.97, and an observed heterozygosity between 0.14 and 1.00. Most primers also yield reproducible results in several other anatid species. These loci comprise a set of autosomal molecular markers for various applications, from moderately polymorphic loci suitable for population studies to highly polymorphic loci for pedigree analysis in waterfowl.
New microsatellite markers for the endemic Mediterranean seagrass Posidonia oceanica
Authors: Alberto F. , Correia L. , Arnaud‐Haond S. , Billot C. , Duarte C. M. , Serrão E. 
Published: May 2003

AbstractThe seagrass Posidonia oceanica is endemic to the Mediterranean Sea, where it plays an important role in coastal ecosystem dynamics. Because of this important role and concerns about the observed regression of some meadows, population genetic studies of this species have been promoted. However, the markers used until now were not polymorphic enough to efficiently assess the level and spatial pattern of genetic variability. Hypervariable molecular markers were obtained by screening a genomic library enriched for microsatellite dinucleotide repeats. Among 25 primer pairs defined, eight amplified polymorphic microsatellites with an encouraging level of variability at the two geographical scales sampled.
Novel tetranucleotide microsatellite DNA markers for the wood frog, Rana sylvatica
Authors: Julian Shannon E. , King Tim L. 
Published: May 2003

AbstractFifteen tetranucleotide microsatellite loci were identified and characterized for wood frogs (Rana sylvatica) collected from three vernal pools in the southeastern US. These markers revealed a high degree of genetic diversity (nine to 34 alleles per locus), heterozygosity (30.6–92.3%) and allelic heterogeneity (69% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.40 and 0.55 and all FST values (0.02–0.05) were statistically significant. Genotypic assignment tests correctly classified 103 of 113 individuals to their respective collection. These markers should prove useful for investigating fine‐scale population structure and metapopulation dynamics.
Laboratory temperature variation is a previously unrecognized source of genotyping error during capillary electrophoresis
Authors: Davison Angus , Chiba Satoshi 
Published: May 2003

AbstractCapillary electrophoresis is being used increasingly for microsatellite genotyping. Here, we report that laboratory temperature greatly affects the estimated allele size, with 5 °C changing the estimated allele size by up to 0.7 bp. With accurate room temperature control, this variation would be relatively unimportant but unfortunately, a questionnaire shows that daily and seasonal laboratory temperature differences are often substantial. One solution is to use a set of reference genotypes, which also greatly assists comparisons between different platforms.
Identification and characterization of microsatellite loci in Betula maximowicziana Regel
Authors: Ogyu K. , Tsuda Y. , Sugaya T. , Yoshimaru H. , Ide Y. 
Published: May 2003

AbstractThe sequences of eight primer pairs of microsatellite loci screened from a genomic library of Betula maximowicziana are reported in this paper. Seven loci showed polymorphism in 44 individuals of the Mitomi population in the central area of Japan and the observed and expected heterozygosities ranged from 0.045 to 0.659 (mean = 0.412) and from 0.044 to 0.642 (mean = 0.397), respectively. Although the one remaining locus is not polymorphic there, it showed variation in other populations. Thus, these eight microsatellite markers might be useful tools for studying the population and ecological genetics of B. maximowicziana.
Microsatellite primers for the Western Pebble‐mound Mouse (Pseudomys chapmani) that show cross amplification for other species of Australian rodent
Authors: Moro D. , P. B. S. Spencer  
Published: May 2003

AbstractWe describe 12 dinucleotide and one tetranucleotide microsatellite loci for the Western Pebble‐mound Mouse (Pseudomys chapmani) that can be amplified with the polymerase chain reaction. All primers produced clear and highly polymorphic amplification patterns containing between seven and 14 alleles and with high expected heterozygosities. The amplification of these primers across seven related conspecifics makes them useful for population genetic studies and conservation work in several of these species.
Characterization of six microsatellite loci in Echium vulgare (Boraginaceae)
Authors: Korbecka G. , Vrieling K. , Squirrell J. , Hale M. L. , Wolff K. 
Published: May 2003

Abstract Echium vulgare is a tetraploid plant with a very low selfing rate in the field. We suspect that cryptic self incompatibility plays a role in this species. In order to show lower success of self pollen/selfed embryos, after pollination with a mixture of self and outcross pollen, a paternity analysis has to be done. For the purpose of such analysis we developed microsatellites in E. vulgare. In this study, we report on six microsatellite loci which are easy to score, polymorphic, with a number of alleles per locus ranging from two to eight and, therefore, suitable for paternity analysis.
Characterization of 10 trinucleotide microsatellite loci in the Critically Endangered Pyrenean yam Borderea chouardii (Dioscoreaceae)
Authors: Segarra‐Moragues J. G. , Palop‐Esteban M. , González‐Candelas F. , Catalán P. 
Published: May 2003

AbstractThe low levels of allozymic variability found in the Critically Endangered Borderea chouardii prompted us to develop microsatellite markers to assess the genetic variability and population structure for the adequate conservation management of this species. A (CTT)n‐enriched partial genomic library was constructed. Ten polymorphic microsatellite loci were isolated from it, rendering 51 alleles in 47 individuals analysed. The allelic pattern observed for all of the loci with more than two alleles suggests that B. chouardii is tetraploid.
Isolation and characterization of microsatellite loci from two inbreeding bark beetle species (Coccotrypes)
Authors: Berg P. R. , Dawson D. A. , Pandhal J. , Kirkendall L. R. , Burke T. 
Published: May 2003

AbstractWe developed 14 microsatellite markers in Coccotrypes carpophagus and 14 in C. dactyliperda. These loci will be used for studying genetic structure and the level of inbreeding in populations in the Canary Islands and Madeira. As a result of long‐term inbreeding, genetic variability is relatively low in these bark beetle species. We found one to five alleles per locus in 29 C. carpophagus and 41 C. dactyliperda from various localities. Eleven of the markers developed for C. carpophagus amplified in C. dactyliperda and seven of the markers developed for C. dactyliperda amplified in C. carpophagus.
Isolation and characterization of microsatellite loci in Greater Sage‐Grouse (Centrocercus urophasianus)
Authors: Taylor S. E. , Oyler‐Mccance S. J. , Quinn T. W. 
Published: May 2003

AbstractPrimers for five polymorphic microsatellite loci were developed for Greater Sage‐Grouse (Centrocercus urophasianus) using an enrichment/detection protocol. The high level of polymorphism (nine to 33 alleles) suggests that these loci will be applicable for investigating mating systems and paternity analysis as well as population genetics. Cross‐species amplification was successful for each locus in at least two other galliform species.
Microsatellites for the Tasmanian devil (Sarcophilus laniarius)
Authors: Jones Menna E. , Paetkau David , Geffen Eli , Moritz Craig 
Published: May 2003

AbstractThe Tasmanian devil (Sarcophilus laniarius), a medium‐sized predator/scavenger, is the largest member of the short‐lived carnivorous marsupial Family Dasyuridae. Now restricted to Tasmania, populations are impacted by habitat clearance and anthropogenic mortality and genetic studies could be of value in informing levels of genetic diversity, mating system, dispersal and the effects of natural and anthropogenic landscape features on gene flow. Microsatellite markers were isolated from a partial, size‐selected genomic library that was enriched for microsatellite sequences. Primer pairs were developed for 11 polymorphic dinucleotide microsatellite loci that conform with Hardy–Weinberg equilibrium and reveal moderate genetic variability across the species range.
A set of polymorphic SSR loci for subarctic willow (Salix lanata, S. lapponum and S. herbacea)
Authors: Stamati K. , Blackie S. , Brown J. W. S. , Russell J. 
Published: May 2003

AbstractWe report the development of seven microsatellite markers from a library enriched with [CA]15, [GA]15, [AAG]8 and [ATG]8. The library was constructed from Icelandic Salix lanata leaf DNA, and seven highly polymorphic single locus products were tested on a range of Scottish and Icelandic S. lanata samples. These seven loci also amplify polymorphic products when tested for cross amplification in a range of Scottish and Icelandic samples of two other subarctic willows (Salix lapponum and Salix herbacea). The microsatellites developed in this study will provide the framework for comparisons with other ecosystem components, and will ultimately inform conservation strategies.
Development of EST‐SSRs from the Alpine Lady‐fern, Athyrium distentifolium
Authors: Woodhead M. , Russell J. , Squirrell J. , Hollingsworth P. M. , Cardle L. , Ramsay L. , Gibby M. , Powell W. 
Published: May 2003

AbstractThe utility of EST‐simple sequence repeats (EST‐SSRs) was evaluated in the fern Athyrium distentifolium. From 1152 frond cDNA clones, 165 microsatellites, including di‐, tri‐, tetra and penta‐nucleotide repeat motifs, were identified. Primer design was possible for 74 of the SSRs; subsequent screening of 10 loci on 186 individuals from six natural populations revealed between two and seven alleles per locus and expected heterozygosity (HE) estimates ranging from 0.027 to 0.809. Eight of these loci were further examined for cross‐species and cross‐generic amplification in other Woodsiaceae species, and polymorphic products were detected. EST‐derived SSRs provide robust, informative and potentially transferable polymorphic markers suitable for biodiversity research.
Microsatellite loci for the North American tortoises (genus Gopherus) and their applicability to other turtle species
Authors: Schwartz T. S. , Osentoski M. , Lamb T. , Karl S. A. 
Published: May 2003

AbstractWe describe the development of nine microsatellite loci from the gopher tortoise (Gopherus polyphemus). Screening 270 individuals, we found these loci to be highly variable (from two to 15 alleles per locus) and thus likely to be applicable in parentage and population‐level analyses for G. polyphemus. All nine loci amplified readily in the other three species of Gopherus as well. This observation, together with successful cross amplification of several loci in two additional families (Cheloniidae, Kinosternidae), underscores their potential utility among turtles in general.
Population genetic differentiation and multiple paternity determined by novel microsatellite markers from the Mountain Log Skink (Pseudemoia entrecasteauxii)
Authors: Stapley Jessica , Hayes Christine M. , Scott Keogh J. 
Published: May 2003

AbstractA total of 15 microsatellite primer pairs were developed from the Mountain Log Skink, Pseudemoia entrecasteauxii. Nine were used to screen 46 individuals from four populations, and a representative from P. spenceri and P. pagenstecheri. Seven of the loci exhibited large allele variation (16–30) and high heterozygosity (0.24–0.82), and the three populations were genetically differentiated. The markers were also used to screen 36 clutches of known maternity and identified high levels of multiple paternity clutches (57%). The primers developed will provide useful markers for the study of population biology and mating system of these lizards.
Isolation and characterization of polymorphic microsatellites in the groundnut seed beetle, Caryedon serratus (Coleoptera, Bruchidae)
Authors: Sembène M. , Vautrin D. , Silvain J. F. , Rasplus J. Y. , Delobel A. 
Published: May 2003

Abstract Caryedon serratus feeds on the seeds of four genera of wild Caesalpinioideae. It recently became a major pest of groundnut in Africa. To determine the extent of gene flow and isolation between wild and groundnut‐feeding populations, we isolated and characterized 10 microsatellite loci in this species. Polymorphism ranged from three to 15 alleles in the 80 individuals collected in four localities in Senegal on five host plants.
Isolation of polymorphic microsatellite markers in the great tit (Parus major minor)
Authors: Kawano Katsura M. 
Published: May 2003

AbstractSix microsatellite loci were developed for a passerine bird, the great tit (Parus major), using two methods. These loci were polymorphic (3–8 alleles per locus) and exhibited expected heterozygosities from 0.45 to 0.77. At one locus the genotypic frequencies deviated significantly from Hardy–Weinberg expectations.
Microsatellites in the bromeliads Tillandsia fasciculata and Guzmania monostachya
Authors: Boneh L. , Kuperus P. , Van Tienderen P. H. 
Published: May 2003

AbstractWe developed microsatellites for the bromeliads Tillandsia fasciculata and Guzmania monostachya, epiphytes of Central‐American montane rain forests. Fragments obtained using amplified fragment length polymorphism (AFLP) pre‐amplification were enriched by hybridization with biotin‐labelled repeat sequences, and subsequently cloned and sequenced. Primers were designed for 15 loci; seven produced interpretable and repeatable amplification products and five were polymorphic in one or both species. We will use these primers to study the population structure of Bromeliaceae in relation to their breeding system as well as to their role in (re‐)colonization of secondary rain forests.
Isolation and characterization of microsatellite loci in the antMyrmica scabrinodis
Authors: Henrich Kai‐Oliver , Sander Anna‐Christine , Wolters Volkmar , Dauber Jens 
Published: May 2003

AbstractFive polymorphic microsatellite loci were developed for the antMyrmica scabrinodisusing a magnetic bead hybridization selection protocol. The number of alleles per locus varied between three and six. Cross‐species amplification of four of the loci yielded positive amplification products in fourMyrmicaspecies, suggesting their general suitability for microsatellite analysis within this taxonomic group.
Fast isolation of microsatellite loci of very diverse repeat motifs by library enrichment in echinoderm species, Amphipholis squamata and Echinocardium cordatum
Authors: Chenuil A. , Le Gac M. , Thierry M. 
Published: May 2003

AbstractWe conceived a microsatellite enrichment protocol in which probes of several repeat motifs are mixed during the whole procedure. The repeats found generally differed from those of the probes and were very diverse, from mononucleotides to pentanucleotides. We tested several modifications with alternative: (i) digestion/ligation buffers; (ii) polymerases; and (iii) purification methods. The simplest methods always worked as well as classical ones or even better, resulting in probably the simplest protocol for isolating microsatellites of diverse motifs from genomes with low microsatellite frequency. The proportion of positive clones, polymorphism levels and cross‐specific amplification of microsatellite loci significantly vary between species.
Identification and characterization of microsatellites loci in Brycon opalinus (Cuvier, 1819) (Characiforme, Characidae, Bryconiae)
Authors: Barroso R. M. , Hilsdorf A. W. S. , Moreira H. L. M. , Mello A. M. , Guimarães S. E. F. , Cabello P. H. , Traub‐Cseko Y. M. 
Published: May 2003

Abstract Brycon species are present in various basins in Brazil and were or still are part of commercial fisheries and aquaculture activities (Ferreira et al. 1996, Mendonça 1994). Despite the importance of this group of fish, natural populations of some Brycon species are endangered (Lins et al. 1997). Here, we describe the characterization of seven microsatellite loci that will be useful for the genetic studies in natural and captive populations for these and other species of Brycon.
Five hundred and fifty microsatellite markers for the study of the honeybee (Apis mellifera L.) genome
Authors: Solignac Michel , Vautrin Dominique , Loiseau Anne , Mougel Florence , Baudry Emmanuelle , Estoup Arnaud , Garnery Lionel , Haberl Michael , Cornuet Jean‐Marie 
Published: May 2003

SummaryMicrosatellites are currently considered the most useful genetic markers with wide applications in genomics, quantitative and population genetics. We present here the structure of the core sequence of 552 microsatellites, together with the sequences of the primers and the length of the sequenced allele. These microsatellites were isolated from several libraries constructed from either fractions of total genomic DNA or from clones of a bacterial artificial chromosome (BAC) library. All 552 loci are polymorphic in the honeybee. Many of them were also successfully amplified in three other species of Apis: A. cerana (58%), A. dorsata (59%) and A. florea (38%). A summary of the variability of 36 loci in the three main evolutionary lineages of A. mellifera is given.
Species‐specific microsatellite loci for the European squid (Loligo vulgaris)
Authors: Guarniero Ilaria , Garoia Flavio , Di Placido Raffaele , Ramsak Andreja , Mannini Piero , Tinti Fausto 
Published: May 2003

AbstractA microsatellite dinucleotide‐enriched library was obtained from the European squid (Loligo vulgaris) and five species‐specific dinucleotide markers were optimized. These markers are highly polymorphic with average expected heterozygosity ranging from 0.706 to 0.927 and allele number ranging from 7 to 17. This set of primers is suitable for population genetic studies.
Polymorphic dinucleotide microsatellites for the Mediterranean angler species (Lophiidae)
Authors: Garoia Flavio , Guarniero Ilaria , Tinti Fausto 
Published: May 2003

AbstractA microsatellite‐enriched genomic library was obtained from black‐bellied angler Lophius budegassa and six polymorphic dinucleotide markers were successfully optimized. These markers showed levels of polymorphism ranging from 0.363 to 0.662 and allele numbers ranging from three to seven. These markers were also found to successfully amplify in the closely related Lophius piscatorius (angler) and thus appear to be useful for genetic stock structure analysis in both species.
Microsatellite loci for the field cricket, Gryllus bimaculatus and their cross‐utility in other species of Orthoptera
Authors: Dawson Deborah A. , Bretman Amanda J. , Tregenza Tom 
Published: May 2003

AbstractWe have isolated 16 microsatellite loci in the field cricket, Gryllus bimaculatus. Nine loci were found to be polymorphic in G. bimaculatus and the number of alleles varied from seven to 14. All 16 loci were tested for amplification in nine other species. In the five species tested belonging to the same subfamily (Gryllinae), a minimum of nine loci amplified. These loci will be used to determine paternity as part of a study to investigate the genetic benefits of polyandry.
The Lorenz theory of beauty
Authors: Fournier Pierre F 
Published: May 2003

SummaryKonrad Lorenz's theory of beauty is developed in detail. To elicit a protective desire, an adult's face must display childlike traits or expressions, called beauty. The emotion of beauty is subjective. The personality and the qualities of the individuals also relevant. In those who feel this emotion, it is largely influenced by the emotions of their earlier life.
Facial blushing treated by sympathetic denervation – longlasting benefits in 831 patients
Authors: Drott Christer , Claes Göran , Rex Lars 
Published: May 2003

SummaryBackground  Severe facial blushing may have a strong negative impact on the quality of life and is one of the cardinal symptoms of social phobia. If traditional therapeutic options such as psychotherapy and pharmacological treatment fail, interruption of the sympathetic innervation to the face offers good results, however long‐term results may not be good.Aim  To investigate whether endoscopic thoracic sympathecotomy (ETS) remains an effective treatment of facial blushing more than one year's follow up.Method  1314 consecutive patients with severe facial blushing were treated with bilateral ETS. The results were evaluated by questionnaire and symptoms assessed with visual analogue scales (0–10).Results  The questionnaire was completed by 831 patients (63%) a mean of 29 months (± 11 days) after surgery. Facial blushing was reduced from 8.8 ± 0.05 to 2.5 ± 0.09, P < 0.0001 by the operation. The quality of life was substantially improved. The main side‐effect was redistribution of sweating from the upper to the lower part of the body (compensatory sweating). Increased sweating of the trunk occurred in 83% of the responses. Overall, 85% of the respondents were satisfied with the result and 15% were to some degree not satisfied.Conclusions  As this is an open study and 37% of patients did not respond to the questionnaire, the results must be viewed with caution. ETS, however, appears to be an effective, safe and lasting surgical method for the treatment of severe facial blushing. Side‐effects, especially compensatory sweating on the trunk and legs are common, and may be severe but only rarely result in the patient regretting the operation.
Facial lipoatrophy: report of five cases after injection of synthetic filler into naso‐labial folds
Authors: André P , Wechsler J , Revuz J 
Published: May 2003

SummaryBackground  Facial lipoatrophies are rare disfiguring syndromes, most commonly characterized by loss of buccal fat pads.Aims  To demonstrate a relationship between facial lipoatrophy and a new synthetic filler device (Profill) used to correct naso‐labial folds.Methods  Five cases of lipoatrophy, appeared 9 months or later after Profill injections. No systematic abnormalities were found. In one case biopsy reported a destruction of adipocytes with intense fibrosis in the hypodermis.Discussion  The culpability of this new biodegradable product is highlighted. To our knowledge, this is the first time this adverse reaction has been reported.Conclusion  Serious adverse reactions may appear months after Profill injection, skin testing is not always predictive or sufficient. Clinical studies with long‐term follow‐up need to be carried out before new filler devices are launched on the market.
Human exposure to solar ultraviolet radiation
Authors: Diffey Brian L 
Published: May 2003

SummarySolar ultraviolet (UV) irradiation depends upon the local UV climate, people's behaviour. Behaviour includes the time spent outdoors and the use of photoprotective agents.In adult life, a British indoor worker in the UK might typically receive 30% of his or her annual UV exposure from sun‐seeking holidays, 40% from summer weekends, 20% from casual weekday exposure between April and September and just 10% from sun exposure during the 6 month period October to March.Whilst climatic factors do influence levels of UV radiation (UVR) at the Earth's surface, it is people's behaviour out of doors that has a much greater impact on personal solar UV irradiation. Methods of personal protection include: avoiding direct sunlight in summer around noon; seeking the shade; wearing clothing absorbs UVR; wearing hats that shade face and neck; and using topical sunscreens.