bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional analysis and treatment of bizarre vocalizations exhibited by an adult with schizophrenia: a replication and extension
Authors: Wilder David A. , White Holly , Yu Michael L. 
Published: January 2003

AbstractA functional analysis suggested that the bizarre vocalizations exhibited by a woman with a diagnosis of schizophrenia were maintained by attention. A treatment consisting of awareness training, competing response training, differential reinforcement of the competing response, and attention extinction was effective in reducing bizarre vocalizations. The use of self‐management based treatment components with this population and target behavior is discussed. Copyright © 2003 John Wiley & Sons, Ltd.
Brief mother–infant treatment: Psychoanalytically informed video feedback
Authors: Beebe Beatrice 
Published: January 2003

AbstractSpecific patterns of interactive regulation documented by microanalytic methods of infant research can be applied to clinical interventions with mothers and infants. A brief treatment model is described that includes face‐to‐face split‐screen videotaping (one camera on each partner) and therapeutic observation of the videotape with the parent. The intervention uses “video feedback” informed by a psychoanalytic approach, including positive reinforcement, modeling, and information giving, as well as interpretation, while watching the videotape. Specific interactions in the areas of attention, arousal, affect, and timing regulation are evaluated. The psychoanalytic intervention links the “story” of the presenting complaints, the “story” seen in the videotape, and the “story” of the parent's own upbringing. An attempt is made to identify specific representations of the baby that may interfere with the parents's ability to observe and process the nonverbal interaction. The mother's powerful experience of watching herself and her baby interact, and our joint attempts to translate the action‐sequences into words, facilitates the mother's ability to “see” and to “remember,” stimulating a rapid integration of the mother's procedural and declarative modes of information‐processing. One treatment case, involving six contacts, is presented to illustrate the approach. By applying the specificity of interactive regulation identified by microanalysis of videotape into the psychodynamic treatment of mother–infant pairs, basic research can be translated into clinical practice. ©2003 Michigan Association for Infant Mental Health.
Putting the pieces together: Maternal depression, maternal behavior, and toddler helplessness
Authors: Kelley Sue A. , Jennings Kay Donahue 
Published: January 2003

AbstractThis study assessed relations between maternal depression, maternal behavior, and helplessness in toddlers. Helplessness was assessed behaviorally in 25‐ and 32‐month‐old toddlers while the toddlers were engaged with an impossible task. Maternal behavior (warmth, negativity, control, intrusiveness) was assessed during a mother–child teaching task when toddlers were 18 and 25 months of age. Mothers who reported more depressive symptoms on the BDI had 32‐month‐old toddlers who displayed more affect‐related helplessness. No direct relations were found between maternal diagnosis of depression and helplessness in toddlers. Few differences emerged in the behavior of depressed and nondepressed mothers while interacting with their toddlers, and few relations were found between maternal behavior alone and toddlers' helplessness. However, results suggest that maternal behavior moderates the relation between maternal depression (diagnosis, recency, and symptoms) and helplessness. ©2003 Michigan Association for Infant Mental Health.
Remote forcing of East African rainfall and relationships with fluctuations in levels of Lake Victoria
Authors: Mistry Vinay V. , Conway Declan 
Published: January 2003

AbstractThis study investigates the climatological variables responsible for fluctuations in Lake Victoria levels, in particular the causal mechanism responsible for a major anomaly that occurred in 1961. A Lake Victoria rainfall series (LVRS) correlates significantly (5%) with Lake Victoria levels and is utilized for subsequent analysis of rainfall variability. Relationships between annual and seasonal (March–May and October–December (OND)) LVRS and a number of tropical and extra‐tropical series were analyzed. The results illustrate the dominance of the El Niño–Southern Oscillation phenomenon in modulating LVRS. The greatest correlation is found between LVRSOND and the Southern Oscillation index (r = −0.39, significant at the 1% level), although the relationship is non‐linear over the course of the century.Velocity potential χ is employed as the principal diagnostic variable. Seasonal composite maps and empirical orthogonal function (EOF) analysis of Indian Ocean region χ‐fields are utilized to test the hypothesis that an Indian Ocean Walker cell is responsible for the anomalous 1961 rainfall episode. Subsequent analysis leads to the rejection of this hypothesis. EOF analysis of OND 200 hPa χ‐fields reveals a number of modes of tropical variability. EOF analysis of the Indian Ocean basin illustrates the emergence of a meridional circulation directed over the Indian Ocean (EOF5). This time series correlates significantly with LVRSOND (r = −0.40, 1% level), although the spatial pattern only explains a small proportion (2.35%) of the total variance.χ analysis for OND over the broader tropical region reveals significant relationships (5%) between OND EOF3* and July–September Sahel rainfall for the preceding season and subsequent year. This relationship may assist future long‐lead seasonal forecast schemes for the Sahel region. Copyright © 2003 Royal Meteorological Society.
North American weather‐type frequency and teleconnection indices
Authors: Sheridan Scott C. 
Published: January 2003

AbstractThe impact of teleconnections upon the surface climate has largely been examined via a response in monthly mean temperature or total precipitation. In this paper, a different approach is undertaken, by examining the response of synoptic weather‐type frequencies to different teleconnection phases.For over 330 stations in the USA and Canada, the Spatial Synoptic Classification scheme has classified each day in each station's period of record into one of seven weather‐type categories, based on thermal, moisture, and other characteristics. The differences in how frequently these different weather types occur in different phases of the North Atlantic Oscillation (NAO) and Pacific–North American (PNA) teleconnection patterns is assessed, for Canadian stations from 1953 to 1993, and for US stations from 1950 to 1999.For PNA, a significant shift in the transitional frequency is observed, suggesting changes in storm track. Concomitantly, a large shift in Dry Polar and Moist Tropical frequencies is observed across the continent. Across the West, in +PNA wintertime months far fewer Dry Polar days are observed. Across the eastern USA, these polar intrusions are more common, and Moist Tropical is diminished significantly.The frequency of the transitional situation is also correlated with NAO phase, with differences as large as a factor of two across much of Canada and the northern USA. In northeastern Canada, there is a large replacement of Moist Polar conditions with Dry Polar conditions during +NAO. Farther south, however, across the eastern USA, both polar weather types occur much less often with +NAO. Although previous research has discovered eastern North American connections to the NAO, this research has shown that the connections often extend into the interior West during much of the year. Particularly strong in the spring, Dry Tropical conditions are much more common with +NAO throughout much of the continent, as far west as the Great Basin. Copyright © 2003 Royal Meteorological Society.
El Niño–southern oscillation influences on the Mahaweli streamflow in Sri Lanka
Authors: Zubair Lareef 
Published: January 2003

AbstractDespite advances over the last two decades in the capacity to predict the evolution of the El Niño–southern oscillation (ENSO) phenomenon and advances in understanding of the relationship between ENSO and climate, there has been little use of climate predictions for water resources management in the tropics. As part of an effort to develop such a prediction scheme, the ENSO influences on streamflow and rainfall in the upper catchment of the Mahaweli river in Sri Lanka were investigated with correlation analysis, composite analysis and contingency tables. El Niño conditions were often associated with decreased annual flows and La Niña with increased flows. The relationship of streamflow and rainfall with the ENSO index of NINO3 contrasted between January to September and October to December. During El Niño episodes the streamflow declines from January to September, but from October to December there is no clear relationship. On the other hand, rainfall shows a clear increase from October to December and declines during January, February, March, July and August. The simultaneous correlations of NINO3 with the aggregate January to September streamflow (r = −0.50), with January to September rainfall (r = −0.44) and with October to December rainfall (r = 0.48) are all significant at the 99% level. The correlation between one‐season‐in‐advance NINO3 with both January to September streamflow and October to December rainfall remained significant at the 99% level.This study demonstrates the potential of using ENSO‐based predictors for a seasonal hydro‐climatic prediction scheme in the Mahaweli basin. It shows the significant contrasts in ENSO influence on rainfall and streamflow due to various hydrological processes. It has demonstrated that the potential for prediction is improved by investigating ENSO influences for the appropriate season for the given river catchment. Copyright © 2003 Royal Meteorological Society.
Herpes Simplex Virus Resistance to Acyclovir and Penciclovir after Two Decades of Antiviral Therapy
Authors: Bacon Teresa H. , Levin Myron J. , Leary Jeffry J. , Sarisky Robert T. , Sutton David 
Published: January 2003

SUMMARY

Acyclovir, penciclovir, and their prodrugs have been widely used during the past two decades for the treatment of herpesvirus infections. In spite of the distribution of over 2.3 × 10
6
kg of these nucleoside analogues, the prevalence of acyclovir resistance in herpes simplex virus isolates from immunocompetent hosts has remained stable at approximately 0.3%. In immuncompromised patients, in whom the risk for developing resistance is much greater, the prevalence of resistant virus has also remained stable but at a higher level, typically 4 to 7%. These observations are examined in the light of characteristics of the virus, the drugs, and host factors.
Indoor Mold, Toxigenic Fungi, andStachybotrys chartarum: Infectious Disease Perspective
Authors: Kuhn D. M. , Ghannoum M. A. 
Published: January 2003

SUMMARYDamp buildings often have a moldy smell or obvious mold growth; some molds are human pathogens. This has caused concern regarding health effects of moldy indoor environments and has resulted in many studies of moisture- and mold-damaged buildings. Recently, there have been reports of severe illness as a result of indoor mold exposure, particularly due to Stachybotrys chartarum. While many authors describe a direct relationship between fungal contamination and illness, close examination of the literature reveals a confusing picture. Here, we review the evidence regarding indoor mold exposure and mycotoxicosis, with an emphasis on S. chartarum. We also examine possible end-organ effects, including pulmonary, immunologic, neurologic, and oncologic disorders. We discuss the Cleveland infant idiopathic pulmonary hemorrhage reports in detail, since they provided important impetus for concerns about Stachybotrys. Some valid concerns exist regarding the relationship between indoor mold exposure and human disease. Review of the literature reveals certain fungus-disease associations in humans, including ergotism (Claviceps species), alimentary toxic aleukia (Fusarium), and liver disease (Aspergillys). While many papers suggest a similar relationship between Stachybotrys and human disease, the studies nearly uniformly suffer from significant methodological flaws, making their findings inconclusive. As a result, we have not found well-substantiated supportive evidence of serious illness due to Stachybotrys exposure in the contemporary environment. To address issues of indoor mold-related illness, there is an urgent need for studies using objective markers of illness, relevant animal models, proper epidemiologic techniques, and examination of confounding factors.
Molecular Host-Pathogen Interaction in Brucellosis: Current Understanding and Future Approaches to Vaccine Development for Mice and Humans
Authors: Ko Jinkyung , Splitter Gary A. 
Published: January 2003

SUMMARYBrucellosis caused by Brucella spp. is a major zoonotic disease. Control of brucellosis in agricultural animals is a prerequisite for the prevention of this disease in human beings. Recently, Brucella melitensis was declared by the Centers for Disease Control and Prevention to be one of three major bioterrorist agents due to the expense required for the treatment of human brucellosis patients. Also, the economic agricultural loss due to bovine brucellosis emphasizes the financial impact of brucellosis in society. Thus, vaccination might efficiently solve this disease. Currently, B. abortus RB51 and B. melitensis REV.1 are used to immunize cattle and to immunize goats and sheep, respectively, in many countries. However, these genetically undefined strains still induce abortion and persistent infection, raising questions of safety and efficiency. In fact, the REV.1 vaccine is quite virulent and apparently unstable, creating the need for improved vaccines for B. melitensis. In addition, Brucella spp. may or may not provide cross-protection against infection by heterologous Brucella species, hampering the acceleration of vaccine development. This review provides our current understanding of Brucella pathogenesis and host immunity for the development of genetically defined efficient vaccine strains. Additionally, conditions required for an effective Brucella vaccine strain as well as the future research direction needed to investigate Brucella pathogenesis and host immunity are postulated.
Herpes Simplex Virus Type 1 and Bovine Herpesvirus 1 Latency
Authors: Jones Clinton 
Published: January 2003

SUMMARYPrimary infection by herpes simplex virus type 1 (HSV-1) can cause clinical symptoms in the peripheral and central nervous system, upper respiratory tract, and gastrointestinal tract. Recurrent ocular shedding leads to corneal scarring that can progress to vision loss. Consequently, HSV-1 is the leading cause of corneal blindness due to an infectious agent. Bovine herpesvirus 1 (BHV-1) has similar biological properties to HSV-1 and is a significant health concern to the cattle industry. Latency of BHV-1 and HSV-1 is established in sensory neurons of trigeminal ganglia, but latency can be interrupted periodically, leading to reactivation from latency and spread of infectious virus. The ability of HSV-1 and BHV-1 to reactivate from latency leads to virus transmission and can lead to recurrent disease in individuals latently infected with HSV-1. During latency, the only abundant HSV-1 RNA expressed is the latency-associated transcript (LAT). In latently infected cattle, the latency-related (LR) RNA is the only abundant transcript that is expressed. LAT and LR RNA are antisense to ICP0 or bICP0, viral genes that are crucial for productive infection, suggesting that LAT and LR RNA interfere with productive infection by inhibiting ICP0 or bICP0 expression. Numerous studies have concluded that LAT expression is important for the latency-reactivation cycle in animal models. The LR gene has recently been demonstrated to be required for the latency-reactivation cycle in cattle. Several recent studies have demonstrated that LAT and the LR gene inhibit apoptosis (programmed cell death) in trigeminal ganglia of infected animals and transiently transfected cells. The antiapoptotic properties of LAT map to the same sequences that are necessary for promoting reactivation from latency. This review summarizes our current knowledge of factors regulating the latency-reactivation cycle of HSV-1 and BHV-1.
Peptides Trap the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Fusion Intermediate at Two Sites
Authors: He Yong , Vassell Russell , Zaitseva Marina , Nguyen Nga , Yang Zhongning , Weng Yongkai , Weiss Carol D. 
Published: January 2003

ABSTRACT
Human immunodeficiency virus type 1 (HIV-1) entry into target cells requires folding of two heptad-repeat regions (N-HR and C-HR) of gp41 into a trimer of N-HR and C-HR hairpins, which brings viral and target cell membranes together to facilitate membrane fusion. Peptides corresponding to the N-HR and C-HR of gp41 are potent inhibitors of HIV infection. Here we report new findings on the mechanism of inhibition of a N-HR peptide and compare these data with inhibition by a C-HR peptide. Using intact envelope glycoprotein (Env) under fusogenic conditions, we show that the N-HR peptide preferentially binds receptor-activated Env and that CD4 binding is sufficient for triggering conformational changes that allow the peptide to bind Env, results similar to those seen with the C-HR peptide. However, activation by both CD4 and chemokine receptors further enhances Env binding by both peptides. We also show that a nonconservative mutation in the N-HR of gp41 abolishes C-HR peptide but not N-HR peptide binding to gp41. These results indicate that there are two distinct sites in receptor-activated Env that are potential targets for drug or vaccine development.
Mouse Polyomavirus Utilizes Recycling Endosomes for a Traffic Pathway Independent of COPI Vesicle Transport
Authors: Mannová Petra , Forstová Jitka 
Published: January 2003

ABSTRACT
Mouse polyomavirus enters host cells internalized, similar to simian virus 40 (SV40), in smooth monopinocytic vesicles, the movement of which is associated with transient actin disorganization. The major capsid protein (VP1) of the incoming polyomavirus accumulates on membranes around the cell nucleus. Here we show that unlike SV40, mouse polyomavirus infection is not substantially inhibited by brefeldin A, and colocalization of VP1 with β-COP during early stages of polyomavirus infection in mouse fibroblasts was observed only rarely. Thus, these viruses obviously use different traffic routes from the plasma membrane toward the cell nucleus. At approximately 3 h postinfection, a part of VP1 colocalized with the endoplasmic reticulum marker BiP, and a subpopulation of virus was found in perinuclear areas associated with Rab11 GTPase and colocalized with transferrin, a marker of recycling endosomes. Earlier postinfection, a minor subpopulation of virions was found to be associated with Rab5, known to be connected with early endosomes, but the cell entry of virus was slower than that of transferrin or cholera toxin B-fragment. Neither Rab7, a marker of late endosomes, nor LAMP-2 lysosomal glycoprotein was found to colocalize with polyomavirus. In situ hybridization with polyomavirus genome-specific fluorescent probes clearly demonstrated that, regardless of the multiplicity of infection, only a few virions delivered their genomic DNA into the cell nucleus, while the majority of viral genomes (and VP1) moved back from the proximity of the nucleus to the cytosol, apparently for their degradation.
Properly Folded Nonstructural Polyprotein Directs the Semliki Forest Virus Replication Complex to the Endosomal Compartment
Authors: Salonen Anne , Vasiljeva Lidia , Merits Andres , Magden Julia , Jokitalo Eija , Kääriäinen Leevi 
Published: January 2003

ABSTRACT

The late RNA synthesis in alphavirus-infected cells, generating plus-strand RNAs, takes place on cytoplasmic vacuoles (CPVs), which are modified endosomes and lysosomes. The cytosolic surface of CPVs consists of regular membrane invaginations or spherules, which are the sites of RNA synthesis (P. Kujala, A. Ikäheimonen, N. Ehsani, H. Vihinen, P. Auvinen, and L. Kääriäinen J. Virol. 75:3873-3884, 2001). To understand how CPVs arise, we have expressed the individual Semliki Forest virus (SFV) nonstructural proteins nsP1 to nsP4 in different combinations, as well as their precursor polyprotein P1234 and its cleavage intermediates. A complex of nsPs was obtained from P123 or P1234, indicating that the precursor stage is essential for the assembly of the polymerase complex. To prevent the processing of the polyprotein and its cleavage intermediates, constructs with the mutation C478A (designated with a superscript CA) in the active site of the protease domain of nsP2 were used. Uncleaved polyproteins containing nsP1 were membrane bound and palmitoylated, and those containing nsP3 were phosphorylated, reflecting properties of authentic nsP1 and nsP3, respectively. Similarly, polyproteins containing nsP1 or nsP2 had enzymatic activities specific for the individual proteins, indicating that they were correctly folded in the precursor state. Uncleaved P12
CA
was localized almost exclusively to the plasma membrane and filopodia, like nsP1 alone, whereas P12
CA
3 and P12
CA
34 were found on cytoplasmic vesicles, some of which contained late endosomal markers. In immunoelectron microscopy these vesicles resembled CPVs in SFV-infected cells. Our results indicate that the nsP1 domain alone is responsible for the membrane association of the nonstructural polyprotein, whereas the nsP1 domain together with the nsP3 domain targets it to the intracellular vesicles.
Arenavirus-Mediated Liver Pathology: Acute Lymphocytic Choriomeningitis Virus Infection of Rhesus Macaques Is Characterized by High-Level Interleukin-6 Expression and Hepatocyte Proliferation
Authors: Lukashevich Igor S. , Tikhonov Ilia , Rodas Juan D. , Zapata Juan C. , Yang Yida , Djavani Mahmoud , Salvato Maria S. 
Published: January 2003

ABSTRACTLymphocytic choriomeningitis virus (LCMV) and Lassa virus can cause hemorrhagic fever and liver disease in primates. The WE strain of LCMV (LCMV-WE) causes a fatal Lassa fever-like disease in rhesus macaques and provides a model for arenavirus pathogenesis in humans. LCMV-WE delivered intravenously or intragastrically to rhesus macaques targets hepatocytes and induces high levels of liver enzymes, interleukin-6 (IL-6), soluble IL-6 receptor (sIL-6R), and soluble tumor necrosis factor receptors (sTNFRI and -II) in plasma during acute infection. Proinflammatory cytokines TNF-α and IL-1β were not detected in plasma of infected animals, but increased plasma gamma interferon was noted in fatally infected animals. Immunohistochemistry of acute liver biopsies revealed that 25 to 40% of nuclei were positive for proliferation antigen Ki-67. The increases in IL-6, sIL-6R, sTNFR, and proliferation antigen that we observe are similar to the profile of incipient liver regeneration after surgical or toxic injury (N. Fausto, Am. J. Physiol. 277:G917-G921, 1999). Although IL-6 was not directly induced by virus infection in vitro, peripheral blood mononuclear cells from acutely infected monkeys produced higher levels of IL-6 upon lipopolysaccharide stimulation than did healthy controls. Our data confirm that acute infection is associated with weak inflammatory responses in tissues and initiates a program of liver regeneration in primates.