bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hemodynamic study of serial double‐filtration plasmapheresis
Authors: Yeh Jiann‐Horng , Chen Wei‐Hung , Chiu Hou‐Chang 
Published: August 2002

AbstractAlthough hypotension is a potential complication for all procedures involving extracorporeal circulation, including plasmapheresis, the effects of serial double‐filtration plasmapheresis (DFP) on hemodynamic status have rarely been reported. Blood pressure (BP) and pulse rate (PR) were prospectively monitored at 30‐minute intervals (baseline, M30, M60, M90, and Ml20) during procedures for 20 myasthenia gravis patients who underwent one course of five consecutive DFP treatments on alternate days, with hemodynamic parameters recorded and analyzed for all sessions. To evaluate the hemodynamic influence of protein loss resulting from serial DFP treatment, additional analysis of serum protein levels including albumin and globulin was conducted before and after the entire course of treatment. Longitudinal analysis on the systolic BP (SBP) changes over five sessions revealed that the SBP at baseline and at M30 dropped significantly during the third and fourth sessions, in comparison to the first (P < 0.05). By contrast, SBP at M120 rose significantly (P < 0.05) after the second session of treatment. A similar trend was revealed for the diastolic BP (DBP) with a significant fall recorded at baseline and at M30 for the fourth session. The PR did not differ significantly during consecutive DFP treatments. Globulin removal rates were correlated significantly with falls in SBP (r2 = 0.250, P = 0.048) and DBP (r2 = 0.405, P = 0.008). However, analogous albumin removal rate was not correlated with these hemodynamic parameters. In conclusion, our results confirm that hypoproteinemia is an important factor for contributing to unstable hemodynamics during serial DFP. J. Clin. Apheresis 17:33–37, 2002. © 2002 Wiley‐Liss, Inc.
Discontinuous‐flow plasmapheresis and patent foramen ovale: a possible cause of paradoxical embolism
Authors: Vicenzini E. , Altieri M. , Di Piero V. , Lenzi G. L. 
Published: August 2002

AbstractA 68‐year‐old woman was admitted for a subacute polyradiculoneuropathy and submitted to PE. A central right jugular venous access was placed after the third PE procedure due to a failing peripheral blood access. During the fourth PE, performed with a discontinuous‐flow system (continuous‐flow used for 3 procedures), she developed multiple embolic ischemic lesions in the left middle cerebral artery (MCA) territory. A thorough cerebrovascular screening showed only a patent foramen ovale (PFO). In our case, a possible increase of the right atrium blood pressure induced by the positive flux of the discontinuous‐flow PE could have been responsible for a paradoxical embolism through the PFO. Plasma exchange (PE) is used in several neurologic disorders. It is commonly thought to be a relatively safe procedure. However, a number of adverse events may occur: paradoxical embolism has not been reported to occur. J. Clin. Apheresis 17:47–48, 2002. © 2002 Wiley‐Liss, Inc.
Collection efficiencies of MNC subpopulations during autologous CD34+ peripheral blood progenitor cell (PBPC) harvests in small children and adolescents
Authors: Witt V. , Fischmeister G. , Scharner D. , Printz D. , Pöttschger U. , Fritsch G. , Gadner H. 
Published: August 2002

AbstractThere is increasing demand for mononuclear cell (MNC) harvests not only for PBPC but also for immune therapies using dendritic cells and donor lymphocytes. We determined the collection efficiencies (CE) of various MNC subpopulations during CD34+ cell harvests using a Fenwal CS 3000 Plus Omnix system in small children and adolescents. The cell content of 140 leukapheresis products (LP) was prospectively evaluated in 45 pretreated patients with solid tumors and hematological malignancies. The median age was 12 years (range 0.8–22), and the median body weight (BW) 43 kg (range 9–92). Depending upon the BW of the patients, the media used for priming were saline (SP) in 86, human albumin (HA, HAP) in 10, and packed red blood cells (BP) in 44 apheresis procedures. The major nucleated cell (NC) fractions collected were monocytes (52% of NC) and CD3+ T cells (26%). The median cell yield for monocytes was 174 × 106/kg (range 24–613) representing a CE of 55%. The median number of CD3+ T cells was 84 × 106/kg (range 5.6–380; CE = 74%). CD34+ cells represented a very small cell fraction of the LP (1.3% of NC), with a median yield of 4.2 × 106/kg (range 0.2–87) and a CE of 63%. The cell yield of various MNCs was significantly correlated with the cell count in the peripheral blood (PB) and with the blood volume processed (ANOVA, P < 0.0001). No influence on the CE was observed for the priming procedure, the patients' age or sex, or the other adaptations used in the harvesting protocol. In conclusion, the Fenwal CS 3000 Plus OMNIX system with the CD34+ cell program and the described adaptations, is also predictably useful for harvest of monocytes or lymphocytes in pediatric patients. We present regression equations that predict the cell yield of various MNC subpopulations in apheresis products. J. Clin. Apheresis 16:161–168, 2001. © 2001 Wiley‐Liss, Inc.
Presurgical plasma exchange for severe factor V deficiency
Authors: Baron Beverly W. , Mittendorf Robert , Baron Joseph M. 
Published: August 2002

AbstractWe report two patients with severe congenital factor V deficiency, one of whom also had a factor V inhibitor, who required correction of their coagulopathy prior to surgical procedures. They underwent plasma exchange (PE) with fresh frozen plasma or solvent/detergent treated plasma (S/DP), with achievement of factor V levels satisfactory for hemostasis for their procedures. PE makes it possible to raise factor levels quickly and sufficiently without volume overload. In addition, transient reduction of inhibitor titers by PE may improve the level of correction achievable during the perioperative period. The advent of S/DP promises to provide an added increment of safety in patients exposed to significant volumes of plasma during PE. J. Clin. Apheresis. 16:29‐30, 2001. © 2001 Wiley‐Liss, Inc.
Prolonged culture of HOS 58 human osteosarcoma cells with 1,25‐(OH)2‐D3, TGF‐beta, and dexamethasone reveals physiological regulation of alkaline phosphatase, dissociated osteocalcin gene expression, and protein synthesis and lack of mineralization
Authors: Siggelkow Heide , Schenck Michael , Rohde Manfred , Viereck Volker , Tauber Simone , Atkinson Michael J. , Hüfner Michael 
Published: August 2002

AbstractCultured rodent osteoblastic cells reiterate the phenotypic differentiation and maturation of osteoblasts seen in vivo. As previously shown, the human osteosarcoma cell line HOS 58 represents a differentiated stage of osteoblast development. The potential of HOS 58 for still further in vitro differentiation suggests the line can serve as a model of osteoblast maturation. Using this cell line, we have investigated the influence of 1,25‐(OH)2‐D3 (D3), TGF‐beta and Dexamethasone (Dex) on proliferation and on the protein and mRNA levels of alkaline phosphatase (AP), procollagen 1 (Col 1), and osteocalcin (Oc), as well as mineralization during 28 days in culture. AP mRNA and protein were highly expressed throughout the culture period with further increase of protein AP activity at constant gene expression levels. A differentiation inhibiting effect of either TGF‐beta or Dex was seen. Col 1 was investigated without the use of ascorbic acid and showed only minor changes during culture time or stimulation. The gene expression for Oc increased continually whereas protein synthesis peaked at confluence and decreased thereafter. TGF‐beta and Dex treatments decreased Oc mRNA and protein levels. Stimulation by D3 was maximal at day 7 with a decrease thereafter. HOS 58 cells showed no mineralization capacity when stimulated with different agents, as measured by energy‐dispersive X‐ray microanalysis. This was not due to absence of Cbfa1 expression. In conclusion, the HOS 58 osteosarcoma cell line represents a differentiated cell line with highly expressed and physiologically regulated AP expression during further differentiation in culture. We observed a dissociation between osteocalcin gene expression and protein secretion which may contribute to the lack of mineralization in this cell line. J. Cell. Biochem. 85: 279–294, 2002. © 2002 Wiley‐Liss, Inc.
Caspase cleavage product lacking amino‐terminus of IκBα sensitizes resistant cells to TNF‐α and TRAIL‐induced apoptosis*
Authors: Kim Ki‐Woo , Kim Byung Ju , Chung Chul‐Woong , Jo Dong‐Gyu , Kim In‐Ki , Song You‐Hyun , Kwon Yun‐Kyung , Woo Ha‐Na , Jung Yong‐Keun 
Published: August 2002

AbstractIn response to a diverse array of signals, IκBα is targeted for phosphorylation‐dependent degradation by the proteasome, thereby activating NF‐κB. Here we demonstrate a role of the cleavage product of IκBα in various death signals. During apoptosis of NIH3T3, Jurkat, Rat‐1, and L929 cells exposed to tumor necrosis factor (TNF)‐related apoptosis‐inducing ligand (TRAIL), Fas, serum deprivation, or TNF‐α, respectively, IκBα was cleaved in a caspase‐dependent manner. In vitro and in vivo cleavage assays and site‐directed mutagenesis showed that caspase‐3 cleaved IκBα between Asp31 and Ser32. Expression of the cleavage product lacking amino‐terminus (1–31), ΔIκBα, sensitized otherwise resistant NIH3T3 fibroblast cells to apoptosis induced by TNF‐α or TRAIL, and HeLa tumor cells to TNF‐α. ΔIκBα was more pro‐apoptotic compared to wild type or cleavage‐resistant (D31E)IκBα mutant and the sensitization elicited by ΔIκBα was as effective as that by the dominant negative mutant, (S32,36A)IκBα, in NIH3T3 cells. ΔIκBα suppressed the transactivation of NF‐κB induced by TNF‐α or TRAIL, as reflected by luciferase‐reporter activity. Conversely, expression of the p65 subunit of NF‐κB suppressed TNF‐α‐, TRAIL‐, and serum deprivation‐induced cell death. On the contrary, ΔIκBα was less effective at increasing the death rate of HeLa cells that were already sensitive to death signals including TRAIL, etoposide, or taxol. These results suggest that ΔIκBα generated by various death signals sensitizes cells to apoptosis by suppressing NF‐κB activity. J. Cell. Biochem. 85: 334–345, 2002. © 2002 Wiley‐Liss, Inc.
VEGF165 mediates formation of complexes containing VEGFR‐2 and neuropilin‐1 that enhance VEGF165‐receptor binding
Authors: Soker Shay , Miao Hua‐Quan , Nomi Masashi , Takashima Seiji , Klagsbrun Michael 
Published: August 2002

AbstractCo‐expression of NRP1 and (VEGFR‐2) KDR on the surface of endothelial cells (EC) enhances VEGF165 binding to KDR and EC chemotaxis in response to VEGF165. Overexpression of NRP1 by prostate tumor cells in vivo results in increased tumor angiogenesis and growth. We investigated the molecular mechanisms underlying NRP1‐mediated angiogenesis by analyzing the association of NRP1 and KDR. An intracellular complex containing NRP1 and KDR was immunoprecipitated from EC by anti‐NRP1 antibodies only in the presence of VEGF165. In contrast, VEGF121, which does not bind to NRP1, did not support complex formation. Complexes containing VEGF165, NRP1, and KDR were also formed in an intercellular fashion by co‐culture of EC expressing KDR only, with cells expressing NRP1 only, for example, breast carcinoma cells. VEGF165 also mediated the binding of a soluble NRP1 dimer to cells expressing KDR only, confirming the formation of such complexes. Furthermore, the formation of complexes containing KDR and NRP1 markedly increased 125I‐VEGF165 binding to KDR. Our results suggest that formation of a ternary complex of VEGF165, KDR, and NRP1 potentiates VEGF165 binding to KDR. These complexes are formed on the surface of EC and in a juxtacrine manner via association of tumor cell NRP1 and EC KDR. J. Cell. Biochem. 85: 357–368, 2002. © 2002 Wiley‐Liss, Inc.
Recombinant Semliki Forest virus enhanced plasminogen activator inhibitor 1 expression and storage in the megakaryocytic cell line MEG‐01
Authors: Chuang Joseph L. , Schleef Raymond R. 
Published: August 2002

AbstractPlatelet plasminogen activator inhibitor I (PAI‐1), a trace α‐granule protein, is a key physiological regulator of fibrinolysis. Because information on the packaging of PAI‐1 into α‐granules during megakaryocytopoiesis may reveal novel approaches for controlling hemostasis, this study investigated basal, plasmid‐mediated, and alphavirus‐mediated PAI‐1 packaging into α‐granules‐like structures in the megakaryocytic cell line MEG‐01. Differentiation of MEG‐01 cells with phorbol myristate acetate (PMA) was observed to result in a four‐fold increase in both secreted and cell‐associated PAI‐1 antigen over a four day period. Subcellular fractionation of PMA‐treated MEG‐01 cells on 45% self‐forming Percoll gradients was employed to separate low density membrane and Golgi‐rich fractions from a high density granule‐containing region. A subsequent 30–60% pre‐formed Percoll gradient was employed to remove contaminating lysosomes from the PAI‐1/glycoprotein IIbIIIa‐containing granules. Electron microscopy showed that these MEG‐01 granules share a similar size distribution (350–600 nm) and morphology to platelet α‐granules. PAI‐1 (40 ng/mg protein) in isolated MEG‐01 storage granules was ∼10% of the levels present in isolated platelet α‐granules. To elevate PAI‐1 production/storage, two expression systems were investigated. Experiments with plasmids encoding PAI‐1 and β‐galactosidase resulted in low transfection efficiency (0.001%). In contrast, Semliki Forest virus (SFV)‐mediated gene transfer increased cellular PAI‐1 by 31‐fold (1,200 ng/106 cells at 10 MOI) in comparison to mock‐infected cells. Pulse‐chase experiments demonstrated that SFV/PAI‐1 mediated gene expression could enhance PAI‐1 storage 6–9‐fold, reaching levels present within platelets. To document the ability of PAI‐1 to be stored in a rapidly releasable form in MEG‐01 cells, we isolated platelet‐like particles from the media conditioned by the cells and examined secretagogue‐induced release of PAI‐1. Particles from SFV/PAI‐1 infected cells display a 5‐fold enhanced secretion of PAI‐1 following treatment with ADP in comparison to particles incubated in the absence of secretagogue. These results suggest that SFV mediated gene expression in MEG‐01 cells provides a useful framework for analyzing the production and storage of α‐granule proteins. J. Cell. Biochem. 82: 277–289, 2001. © 2001 Wiley‐Liss, Inc.
Caveolae and the organization of carbohydrate metabolism in vascular smooth muscle
Authors: Lloyd Pamela G. , Hardin Christopher D. 
Published: August 2002

AbstractWe have previously found that glycolysis and gluconeogenesis occur in separate “compartments” of the VSM cell. These compartments may result from spatial separation of glycolytic and gluconeogenic enzymes (Lloyd and Hardin [1999] Am J Physiol Cell Physiol. 277:C1250‐C1262). We have also found that an intact plasma membrane is essential for compartmentation to exist (Lloyd and Hardin [2000] Am J Physiol Cell Physiol. 278:C803‐C811), suggesting that glycolysis and gluconeogenesis may be associated with distinct plasma membrane microdomains. Caveolae are one such microdomain, in which proteins of related function colocalize. Thus, we hypothesized that membrane‐associated glycolysis occurs in association with caveolae, while gluconeogenesis is localized to non‐caveolae domains. To test this hypothesis, we disrupted caveolae in vascular smooth muscle (VSM) of pig cerebral microvessels (PCMV) with β methyl‐cyclodextrin (CD) and examined the metabolism of [2‐13C]glucose (a glycolytic substrate) and [1‐13C]fructose 1,6‐bisphosphate (FBP, a gluconeogenic substrate in PCMV) using 13C nuclear magnetic resonance spectroscopy. Caveolar disruption reduced flux of [2‐13C]glucose to [2‐13C]lactate, suggesting that caveolar disruption partially disrupted the glycolytic pathway. Caveolae disruption may also have resulted in a breakdown of compartmentation, since conversion of [1‐13C]FBP to [3‐13C]lactate was increased by CD treatment. Alternatively, the increased [3‐13C]lactate production may reflect changes in FBP uptake, since conversion of [1‐13C]FBP to [3‐13C]glucose was also elevated in CD‐treated cells. Thus, a link between caveolar organization and metabolic organization may exist. J. Cell. Biochem. 82:399–408, 2001. © 2001 Wiley‐Liss, Inc.
Constitutive and heat‐shock induced expression of Hsp70 mRNA during chicken testicular development and regression
Authors: Mezquita Belén , Mezquita Jovita , Durfort Mercè , Mezquita Cristóbal 
Published: August 2002

AbstractThe constitutive and heat shock induced expression of Hsp70 mRNA was investigated in normal adult chicken testis and in adult testis after testicular regression induced by diethylstilbestrol (DES) treatment. In addition to the canonical form of Hsp70 mRNA, we have detected transcripts with an extended 5′UTR and transcripts containing, in the 5′UTR, sequences of the 18S ribosomal RNA. Hsp70 was expressed in unstressed male gonads in adult and regressed testis, being the expression much lower in regressed testis. Upon heat shock at 44°C or 46°C, Hsp70 was highly induced in both tissues. However, when testicular seminiferous tubules were incubated at the chicken internal temperature of 39°C, no induction of Hsp70 was observed in mature testis, while the expression markedly increased in regressed testis. Induction at 39°C was completely inhibited in the presence of 6 mM aspirin. Aspirin in the range 3–10 mM decreases the expression of Hsp70 in unstressed and stressed testicular cells, in striking contrast with the effect observed in other tissues as liver. These data suggest that the expression of Hsp70 is regulated in a specific manner in chicken testis and particularly in the male gonad undergoing regression. J. Cell. Biochem. 82:480–490, 2001. © 2001 Wiley‐Liss, Inc.
Cellular and molecular properties associated with osteosarcoma cells
Authors: Benayahu D. , Shur I. , Marom R. , Meller I. , Issakov J. 
Published: August 2002

AbstractOsteosarcoma cells are recognized by abnormal function that causes a primary bone tumor. Osteosarcoma cells U2OS and SAOS‐2 were analyzed for the expression of cell surface markers. High expression was quantified for hyaloronidase receptor (CD‐44) > moderate for integrins (CD‐51 and ‐61), > and lower for selectins (CD‐62). High mitotic capacity were demonstrated by gene expression (measured by RT‐PCR) and the protein level (measured by FACS) for cFOS, cMYC, and cJUN. The basic definition of osteosarcoma is excessive production of pathological osteoid. Expression of mRNA for matrix genes osteocalcin, osteonectin, and biglycan was studied. Osteocalcin and osteonectin were detected in RNA from primary cultured marrow stromal, trabecular bone cells, and osteosarcoma cell lines (U2OS, SAOS‐2). mRNA for biglycan was detected only in primary cells and MG‐63 cell line and was undetectable in RNA from U2OS, SAOS‐2 osteosarcoma cell lines and by RNA extracted from bone biopsies of osteosarcoma patients. The absence of biglycan message observed in osteosarcoma samples provides evidence for the alterations in the extra cellular matrix which result with non‐mineralized osteoid produced by the osteosarcoma cells. J. Cell. Biochem. 84: 108–114, 2002. © 2001 Wiley‐Liss, Inc.
Effect of fibroblast growth factors 1, 2, 4, 5, 6, 7, 8, 9, and 10 on avian chondrocyte proliferation
Authors: Praul Craig A. , Ford Bonnie C. , Leach Roland M. 
Published: August 2002

AbstractIt has been demonstrated that fibroblast growth factor receptors are key regulators of endochondral bone growth. However, it has not been determined what fibroblast growth factor ligand(s) (FGFs) are important in this process. This study sought to determine whether FGFs 1, 2, 4, 5, 6, 7, 8, 9, and 10 were capable of stimulating avian chondrocyte proliferation in vitro. We have found that FGFs 2, 4, and 9 strongly stimulate avian chondrocyte proliferation while FGFs 6 and 8 stimulate proliferation to a lesser extent. RT‐PCR indicates that FGF‐2 and FGF‐4 are expressed in the postnatal avian epiphyseal growth plate (EGP) while FGF‐8 and FGF‐9 are not. Thus, FGF‐2 and FGF‐4 stimulate chondrocyte proliferation and are both present in the EGP. This suggests that FGF‐2 and FGF‐4 may be important ligands, in vivo, for the regulation of endochondral bone growth. These observations coupled with our observation that multiple avian FGF receptors (Cek1, Cek2, Cek3, and FREK) are expressed in proliferative chondrocytes highlights the complexity of FGF signaling pathways in postnatal endochondral bone growth. J. Cell. Biochem. 84: 359–366, 2002. © 2001 Wiley‐Liss, Inc.
Incidence matrices and inequalities for combinatorial designs
Authors: Raghavarao D. , Shrikhande S.S. , Shrikhande M.S. 
Published: August 2002

AbstractIn this paper we use incidence matrices of block designs and row–column designs to obtain combinatorial inequalities. We introduce the concept of nearly orthogonal Latin squares by modifying the usual definition of orthogonal Latin squares. This concept opens up interesting combinatorial problems and is expected to be useful in planning experiments by statisticians. © 2002 John Wiley & Sons, Inc. J Combin Designs 10: 17–26, 2002
Development of two ELISA for estrogen and progesterone receptor with sufficient sensitivity for fine needle aspirate and core biopsy
Authors: Sun C.‐F. , Wu T.‐L. , Tsao K.‐C. , Wu J.T. 
Published: August 2002

AbstractThe quantitative determination of estrogen and progesterone receptors (PR and ER) in breast tumor cytosol has been routinely performed in clinical laboratories to aid in the selection between hormonal and chemotherapy and also to predict prognosis. However, the small amount of tissue available from the increasingly popular fine‐needle aspiration and core biopsies from breast cancer patients requires more sensitive immunoassays for receptor quantification. We have developed two sensitive immuno‐assays for ER and PR on microplate with the use of recently available anti‐ER and anti‐PR antibodies of higher affinity and a powerful signal magnification agent, namely Amdex. The calibrator was a pooled breast tumor cytosol used as calibrator and calibrated against Abbott kits. The protein concentration of the cytosol and the upper normal cutoffs for our assays were reduced to approximately 0.2 mg/mL and 3 fmol/0.2 mg/mL, respectively. Both assays have sensitivities close to 1 fmol/mL, which are sufficiently sensitive for the receptor quantification in fine‐needle aspiration biopsies and cord biopsies of breast tumor. J. Clin. Lab. Anal. 15:138–143, 2001. © 2001 Wiley‐Liss, Inc.
Counteracting the effects of invisibility in work with lesbian patients
Authors: Saari Carolyn 
Published: August 2002

AbstractLesbian women frequently experience “invisibility,” the failure of others to recognize the significance of their sexuality and partnership relations. Such invisibility can have deleterious effects, such as a reduced ability to relate life stories to others and thereby to extend and integrate aspects of identity and its healthy complexity. Although this invisibility often is intertwined with coming‐out phenomena, it continues to exist in many social contexts throughout life. Therefore, it is important for psychotherapists to recognize and understand the concept of invisibility so that they do not perpetuate it within the therapy. Several case vignettes are used as examples of how invisibility can affect a woman's life and functioning and can be counteracted in therapy. © 2001 John Wiley & Sons, Inc. J Clin Psychol/In Session 57: 645–654, 2001.