bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pan-cancer analysis identifies RNF43 as a prognostic, therapeutic and immunological biomarker
Authors: Xu Yingting , Lin Zhengjun , Ji Yuqiao , Zhang Chen , Tang Xianzhe , Li Chuan , Liu Tang 
Published: October 2023

Abstract
Background
RING finger protein 43 (RNF43), an E3 ubiquitin ligase, is a homologous gene mutated in several cancers. However, the pan-cancer panoramic picture of RNF43 and its predictive value for tumor immune phenotypes and immunotherapeutic efficacy are still largely unclear. Our study aims to clarify the functions of RNF43 in predicting the prognosis, immune signature, and immunotherapeutic efficacy in pan-cancer.

Methods
By using RNA-seq, mutation, and clinical data from the TCGA database, the expression levels and prognostic significance of RNF43 in pan-cancer were analyzed. The genetic alteration characteristics of RNF43 were displayed by the cBioPortal database. Gene Set Enrichment Analysis (GSEA) was performed to investigate the potential biological functions and signaling pathways modulated by RNF43 in cancers. The relationship of RNF43 expression with immune cell infiltration, and immune modulators expression was interpreted by the ESTIMATE algorithm, CIBERSORT algorithm, and TISIDB database. The correlations between RNF43, microsatellite instability (MSI), and tumor mutation burden (TMB) were also investigated. Furthermore, the predictive value of RNF43 for immunotherapeutic efficacy and drug sensitivity was further illustrated. Besides, immunohistochemistry (IHC) was employed to validate the expression of the RNF43 in different cancer types by our clinical cohorts, including patients with lung cancer, sarcoma, breast cancer, and kidney renal clear cell carcinoma.

Results
The results demonstrated that RNF43 was abnormally expressed in multiple cancers, and RNF43 is a critical prognosis-related factor in several cancers. RNF43 was frequently mutated in several cancers with a high frequency of 4%, and truncating mutation was the most frequent RNF43 mutation type. RNF43 expression was linked to the abundance of several immune cell types, including CD8+ T cells, B cells, and macrophages within the tumor immune microenvironment. Furthermore, RNF43 expression was significantly correlated with the efficacy of anti-PD-1/PD-L1 treatment, and it could predict the sensitivity of various anti-cancer drugs. Finally, IHC explored and validated the different expression levels of RNF43 in different cancers by our clinical samples.

Conclusion
Our results first present the expression pattern and the mutation signature of RNF43, highlighting that RNF43 is an important prognostic biomarker in pan-cancer. Furthermore, RNF43 seems to be a critical modulator in the tumor immune microenvironment and can function as a promising biomarker for predicting the immunotherapeutic efficacy of anti-PD-1/PD-L1 treatment, and drug sensitivity in cancer treatment.
Is it possible to quantify the current resilience level of an agri-food system? A review of the literature
Authors: Tebaldi Letizia , Vignali Giuseppe 
Published: October 2023

AbstractNowadays being resilient is a requirement of all companies and more in general supply chains, as a consequence of the frequent disruptions which repeatedly affect systems and challenge markets from different sides. But how to state whether a company and its related supply chain are resilient or not? To address the present issue, a literature review was carried out on documents proposing quantitative tools or metrics for quantifying the resilience level of an agri-food supply chain, which is a specific field subjected to several threats and accordingly deserving attention. Due to the limited number of documents retrieved (i.e., 26 articles), stressing the gap to be filled in literature, mainly bibliometric analyses were performed on the sample, but contents were also deepened, resuming the different tools available at present. Results reflect the call for the development of models aiming at assessing the resilience of these systems before disruptions and non-controlled events occur; moreover, the industrial level turned out to be neglected, given the fact that all the studies deal with the farm stage (and in general agricultural activities).
Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models
Authors: Rikken Gijs , Meesters Luca D. , Jansen Patrick A. M. , Rodijk-Olthuis Diana , van Vlijmen-Willems Ivonne M. J. J. , Niehues Hanna , Smits Jos P. H. , Oláh Peter , Homey Bernhard , Schalkwijk Joost , Zeeuwen Patrick L. J. M. , van den Bogaard Ellen H. 
Published: October 2023

Abstract
Background
Following descriptive studies on skin microbiota in health and disease, mechanistic studies on the interplay between skin and microbes are on the rise, for which experimental models are in great demand. Here, we present a novel methodology for microbial colonization of organotypic skin and analysis thereof.

Results
An inoculation device ensured a standardized application area on the stratum corneum and a homogenous distribution of bacteria, while preventing infection of the basolateral culture medium even during prolonged culture periods for up to 2 weeks at a specific culture temperature and humidity. Hereby, host-microbe interactions and antibiotic interventions could be studied, revealing diverse host responses to various skin-related bacteria and pathogens.

Conclusions
Our methodology is easily transferable to a wide variety of organotypic skin or mucosal models and different microbes at every cell culture facility at low costs. We envision that this study will kick-start skin microbiome studies using human organotypic skin cultures, providing a powerful alternative to experimental animal models in pre-clinical research.
Spatio-temporal disparities of Clonorchis sinensis infection in animal hosts in China: a systematic review and meta-analysis
Authors: Liu Kai , Tan Jing , Xiao Lu , Pan Rui-Tai , Yao Xiao-Yan , Shi Fu-Yan , Li Shi‐Zhu , Li Lan‐Hua 
Published: October 2023

Abstract
Background
Clonorchissinensis, one of the most important food-borne zoonotic trematodes, remains prevalent in China. Understanding its infection status in animals is crucial for controlling human clonorchiasis. Here we conducted a systematic review and meta-analysis to focus on the spatio-temporal disparities of C.sinensis infection in animals in China.

Methods
Data on C.sinensis prevalence in snails, the second intermediate hosts, or animal reservoirs in China were extracted from electronic databases including PubMed, Embase, Web of Science, Chinese Wanfang database, CNKI, VIP, and China Biomedical Literature database. A random-effects meta-analysis model was utilized to estimate the pooled prevalence in each of the above animal hosts. Subgroup analysis and multivariable meta-regression were performed to explore potential sources of heterogeneity across studies and compare the temporal disparity of infection rates between high and low epidemic areas. Scatter plots were used to depict the biogeographical characteristics of regions reporting C.sinensis infection in animals.

Results
The overall pooled prevalence of C.sinensis was 0.9% (95% CI: 0.6–1.2%) in snails, 14.2% (12.7–15.7%) in the second intermediate host, and 14.3% (11.4–17.6%) in animal reservoirs. Prevalence in low epidemic areas (with human prevalence < 1%) decreased from 0.6% (0.2–1.2%) before 1990 to 0.0% (0.0–3.6%) after 2010 in snails (P = 0.0499), from 20.3% (15.6–25.3%) to 8.8% (5.6–12.6%) in the second intermediate hosts (P = 0.0002), and from 18.3% (12.7–24.7%) to 4.7% (1.0–10.4%) in animal reservoirs. However, no similar decrease in prevalence was observed in high epidemic areas (with human prevalence ≥ 1.0%). C.sinensis infections were predominantly reported in areas with altitudes below 2346 m and annual cumulative precipitation above 345 mm and were mostly concentrated in eastern China.

Conclusions
There are spatio-temporal disparities in the animal infections of C.sinensis in different areas of China. Animal infections are primarily concentrated in regions with low altitude and high precipitation. The results suggest that implementing One Health-based comprehensive measures targeting both humans and animals, especially in high epidemic areas, is essential for successful eradication of C.sinensis in China.
Evaluation of effectiveness of school-based nutrition education in improving the consumption of pulses-based food among female adolescents in Northwest Ethiopia: a cluster randomized controlled trial
Authors: Mekonnen Fantahun Ayenew , Biks Gashaw Andargie , Azale Telake , Mengistu Netsanet Worku 
Published: October 2023

AbstractBackgroundProtein undernutrition is a prevalent health problem in Ethiopia severely affecting the reproductive outcome of women. This is mainly because of inadequate consumption of protein due to the high cost of animal-origin food and the lack of knowledge about the benefits and the methods of preparation of pulses-based foods. Therefore, this trial was conducted to evaluate the effectiveness of nutrition education in improving the consumption of pulses-based foods among female adolescents.MethodsA two-arm pragmatic cluster randomized controlled trial was conducted among female adolescents in Northwest Ethiopia. Clusters were schools assigned into intervention and control groups by cluster randomization. The trial participants were female adolescents. The intervention was pulses-based nutrition education, and the comparator was the usual dietary practice of adolescent girls. The education was delivered over four weeks on a 45–60 min session per week basis. The primary outcome of the intervention was pulses-based food consumption, and the secondary outcomes were knowledge and attitude about pulses food. Data on the outcome and the confounding variables were collected at baseline and end-line of the intervention. The analysis was based on intention-to-treat analysis, and a log-binomial logistic regression model was fitted to the data to calculate relative risk with the correspondingpvalue adjusted for baseline characteristics. The intervention was considered effective when thepvalue was < 0.05.ResultsA total of 269 intervention and 278 control participants from the four clusters completed the trial making response rates of 92.1% and 95.2%, respectively. The pulses-based nutrition education enabled participants in the intervention group to maintain their pulses-based food consumption state, while participants in the control group significantly reduced their consumption by about threefold [ARR; 95% CI 2.99 (1.87, 4.79)] from harvesting to non-harvesting season. The consumption of pulses-based food was higher by 16% among the intervention participants as compared to the control participants [ARD; 95% CI 0.16 (0.10, 0.21)].ConclusionPulses-based nutrition education is effective in improving the consumption of pulses-based food among female adolescents. Therefore, policies and strategies are required to integrate this intervention in the school nutrition program.Trial registration: The trial was registered in the Pan African Clinical Trials Registry (PACTR202111813445259) on 02 November 2021.
Chemical Structure Evaluations of Amine Hardeners to Ensure and Predict the Performance of Wet Adhesion of Epoxies
Authors: Nakamura Yasuyuki , Hibi Yusuke , Naito Kimiyoshi , Yamamoto Norie , Hanamura Misato 
Published: October 2023

Abstract
The relationship between the chemical structure and performance of a water-sorbed epoxy adhesive (wet adhesion) provides fundamental data for epoxy adhesives for application in wet and underwater environments. However, data on the effect of the chemical structure on wet adhesion remains insufficient. This study systematically examined the wet adhesion strengths of epoxies comprising bisphenol A diglycidyl ether and various amines. The use of numerical parameters quantifying the features of the chemical structure and physicochemical properties via theoretical calculations to analyze the correlation between wet adhesion and the chemical structure of amine yielded clear linear relationships. This enabled the extraction of the amine molecular structural features that were superior in wet adhesion, in addition to quantification of the certainties of the features contributing to the physical properties. Furthermore, a prediction model for wet adhesive strength was prepared using machine-learning least absolute shrinkage and selection operator regression analysis. The model exhibited a reasonable accuracy, even using only 14 experimental values, and its effectiveness was verified experimentally. This process facilitates the rational design and selection of amine hardeners for preparing epoxies with excellent performance in wet conditions and underwater environments.
If You Build It, Will They Come? A Quasi-experiment of Sidewalk Improvements and Physical Activity
Authors: Knell Gregory , Durand Casey P. , Shuval Kerem , Kohl Harold W. , Salvo Deborah , Olyuomi Abiodun , Gabriel Kelley Pettee 
Published: October 2023

ABSTRACT
Improving sidewalks could lead to more physical activity through improved access, while providing a safe and defined space to walk. However, findings on the association between sidewalks and physical activity are inconclusive. The purpose of this study was to examine changes in self-reported and accelerometer-derived physical activity associated with living near recently improved sidewalks in a diverse, community-based sample from the Houston Travel Related Activity in Neighborhoods Study. Data are from 430 adults and include baseline and first annual follow-up (2014–2017). Fully adjusted, two-step regression models were built to test the hypothesis that living near (within 250 m) an improved sidewalk was associated with greater levels of physical activity compared with not living near an improved sidewalk. Most participants were female, were non-Hispanic black, had low income, and had low education, and nearly half lived near at least one improved sidewalk. After adjustment, among participants reporting some physical activity, living near two sidewalk improvements was associated with 1.6 times more minutes per week of walking and leisure-time physical activity compared with those not living near a sidewalk improvement (P < 0.05). On the basis of accelerometry, which does not specifically quantify domain-specific physical activity, there were no significant associations. In conclusion, although these mixed findings warrant further research, results suggest that improving sidewalks may have an effect on participants' physical activity. Nonspecific definitions of sidewalk improvements could be contributing to type 1 error. Future work should also examine behavioral interventions alongside changes to the built environment to determine the effects on physical activity.
Quick Response Under Strategic Manufacturer
Authors: Chen Jiguang , Hu Qiying , Shi Duo , Zhang Fuqiang 
Published: October 2023

Problem definition: Quick response is a classic operations strategy that allows a retailer to place a rapid replenishment order during the selling season using information learned from early sales. The benefits of quick response are widely studied in the literature under the condition that the manufacturer’s wholesale prices are exogenously given. Motivated by the practice of emerging small and medium-sized enterprise (SME) fashion brands, this paper revisits the value of quick response for a retailer when a manufacturer can strategically set its wholesale prices. Methodology/results: We develop a game-theoretic model consisting of one manufacturer and one retailer. In contrast to the traditional quick response setting, the manufacturer can dynamically adjust wholesale prices for both regular and replenishment orders. First, we investigate whether and when quick response still benefits the retailer. We find that, under low or significantly high demand uncertainties, the firms share a common preferred ordering strategy, and quick response benefits the retailer as well as the supply chain. But, under moderately high demand uncertainty, the retailer’s favored ordering strategy conflicts with the manufacturer’s interest; as a result, the manufacturer would set wholesale prices to counter the retailer’s ordering strategy, which makes quick response detrimental to the retailer. Second, we search for mechanisms that can resolve this conflict and restore the beneficial effect of quick response. We show that letting the manufacturer commit to wholesale prices up front is ineffective in fixing the problem. However, if the retailer can propose a take-it-or-leave-it wholesale price for the replenishment order (possibly with the replenishment quantity) once the regular wholesale price is set, then quick response leads to a win–win outcome for both firms. Managerial implications: The findings caution retailers with weak power (e.g., SMEs) when adopting quick response, especially when facing moderately high demand uncertainties. The retailer, although weak, should be aware of the retailer’s natural ability to propose replenishment terms because, otherwise, the retailer can always forgo quick response; this opens up an opportunity to design more favorable arrangements. Funding: The work of J. Chen was supported by the National Natural Science Foundation of China [Grants 72171202, 72232007]; the work of Q. Hu was supported by the National Natural Science Foundation of China [Grants 72271057, 72091211]; the work of D. Shi was supported by the National Natural Science Foundation of China [Grant 72102205] and Shenzhen Stable Support Program for Higher Education Institutions; the work of F. Zhang was supported by the National Natural Science Foundation of China [Grants 71929201, 72131004]. Supplemental Material: The online supplement is available at https://doi.org/10.1287/msom.2021.0561 .
Sobrecarga emocional del personal sanitario del Servicio Navarro de Salud durante la pandemia del SARS-CoV-2
Authors: Serrano-Domínguez Silvia , Lahera-Martín Matilde , Ibáñez-Beroiz Berta , Alsúa-Fernández Concepción , Ordoqui-García Elena , Furtado-Eraso Beatriz , Mollov Asan , Asenjo-Redín Belén 
Published: October 2023

Objetivo: Los trabajadores de la salud se enfrentaron a niveles sin precedentes de carga emocional relacionada con la pandemia de la COVID-19. Este estudio pretende conocer la autopercepción del estado de sobrecarga emocional de los profesionales del Servicio Navarro de Salud-Osasunbidea (SNS-O) en dos momentos durante la pandemia y sus factores asociados.
Método: Estudio transversal mediante el uso de un cuestionario que incluye la Escala de Autoevaluación de Sobrecarga Emocional, enviado por correo electrónico a todos los profesionales sanitarios del SNS-O en dos momentos de la pandemia, en octubre de 2020 y octubre de 2021. Los resultados de la escala global y de las subescalas ‘“miedos-ansiedad”’ y ‘“respuesta afectiva”’ se compararon entre los dos momentos de medición según puesto de trabajo, ámbito, área y servicio, mediante modelos ANCOVA?.
Resultados: Participaron en la encuesta de 2020 1.485 (11,3%) sanitarios y 950 (7,3%) en la encuesta de 2021 de. La diferencia media para el score global entre 2020 y 2021 fue -1.87 (95% IC: -2.38, -1.35), indicando una reducción de la sobrecarga emocional con la evolución de la pandemia, con algunas diferencias entre categorías profesionales.
Conclusiones: El año 2020 tuvo gran impacto en la sobrecarga emocional que disminuyó al año siguiente. Uno de los mayores temores del personal sanitario durante su exposición laboral a enfermedades infecciosas epidémicas sigue siendo el miedo a contraer la enfermedad y transmitirla a sus familiares y entorno social. Conforme aumenta la proximidad con el paciente y su entorno, incrementa la probabilidad de afectación psicoemocional.
Desarrollo de funciones de la enfermería del trabajo en la vigilancia de la salud laboral por exposición a tóxicos: el tolueno como ejemplo
Authors: De Pedro Jiménez Domingo 
Published: October 2023

Introducción: El tolueno, hidrocarburo aromático presente en varios productos, se absorbe principalmente por vía respiratoria y afecta diferentes sistemas corporales. Los enfermeros/as del trabajo son clave para detectar exposiciones agudas y crónicas. Además, son responsables de recolectar y procesar las muestras para su biomonitorización. Sin embargo, la información sobre los requisitos de recogida y manipulación, así como de intervenciones enfermeras es escasa y heterogénea.
Objetivo: Revisar la bibliografía disponible sobre el tolueno y la enfermería del trabajo, identificar requisitos de recogida y procesamiento de muestras, así como los signos y síntomas de exposición y relacionarlos con diagnósticos e intervenciones enfermeras.
Métodos: Se realizó una revisión bibliográfica aplicando la estrategia PICO, no limitada por tipo de documento, fecha o idioma en PubMed, Web of Science y Scopus. Se clasificaron los signos y síntomas según sistema y prueba de enfermería realizada durante el examen de salud, identificando diagnósticos e intervenciones enfermeras.
Resultados: No se encontraron estudios sobre tolueno y enfermería del trabajo. De 60 citas identificadas, 6 artículos señalaban requisitos de recogida de muestras. Se identificaron signos y síntomas de acuerdo a las pruebas de enfermería y se relacionaron con diagnósticos e intervenciones de enfermería.
Conclusiones: La presencia de la enfermería del trabajo en la literatura científica sobre tolueno es escasa. Los criterios de recogida de muestras son heterogéneos y limitados. No se han encontrado estudios que relacionen signos y síntomas de exposición al tolueno con diagnósticos e intervenciones enfermeras. Se propone un cuadro relacional novedoso. Son necesarios estudios de toxicología laboral desde la perspectiva de la enfermería del trabajo.
Using Open-Path Dual-Comb Spectroscopy to Monitor Methane Emissions from Simulated Cattle Grazing
Authors: Morris Lindsay C. , Weerasekara Chinthaka , Malarich Nathan , Giorgetta Fabrizio R. , Herman Daniel I. , Cossel Kevin C. , Newbury Nathan R. , Owensby Clenton E. , Welch Stephen M. , DePaola Brett D. , Coddington Ian , Santos Eduardo A. , Washburn Brian R. 
Published: October 2023

A controlled methane release study was performed using dual comb spectroscopy to simulate cattle emissions at a grazing system. The DCS system can measure a 10 nmol/mol methane enhancement above the atmospheric background concentration.
Work of the US Government and not subject to copyright.
New Biomarkers for Cardiovascular Disease
Authors: Kim Stephanie J. , Mesquita Fernanda C. P. , Hochman-Mendez Camila 
Published: October 2023

Abstract
Cardiovascular disease is the leading cause of death and disability worldwide. Early detection and treatment of cardiovascular disease are crucial for patient survival and long-term health. Despite advances in cardiovascular disease biomarkers, the prevalence of cardiovascular disease continues to increase worldwide as the global population ages. To address this problem, novel biomarkers that are more sensitive and specific to cardiovascular diseases must be developed and incorporated into clinical practice. Exosomes are promising biomarkers for cardiovascular disease. These small vesicles are produced and released into body fluids by all cells and carry specific information that can be correlated with disease progression. This article reviews the advantages and limitations of existing biomarkers for cardiovascular disease, such as cardiac troponin and cytokines, and discusses recent evidence suggesting the promise of exosomes as cardiovascular disease biomarkers.