bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSIII-3 Correlations Between Body Surface and Rectal Temperature of Newly Weaned Sows
Authors: Willard Naomi C , Moest Nicole K , Vande Pol Katherine D , Shull Caleb M , Pimpinella Rich , Grein Christoph , Ellis Mike 
Published: October 2023

Abstract
There is interest in using body temperature changes in sows as an indicator of the onset of estrus. However, the optimum location to measure body temperature has not been established. The objective of this study was to evaluate the relationships between sow body surface temperature (SBST) measured at various locations and rectal temperature. The study involved 98 multiparous sows weaned in 6 groups between September and November. Sows were housed in individual stalls and were checked daily for standing reflex from day 3 to 7 post-weaning. Temperature measurements were taken daily for 10 days post-weaning [at 06:15 (feeding time), 07:00, 07:30 (heat checking time), 09:00, 11:00, 13:00, 15:00, 17:00, 19:00, 21:00, and 23:00]. Surface temperature was measured using a handheld thermal camera at 4 locations: outer ear, back of the head, vulva, and ham. Rectal temperature was measured using a clinical thermometer. The ambient temperature in the room was measured at the same times as the animal measurements were taken. The PROC CORR procedure of SAS was used to determine correlations between SBST, rectal temperature, and average room temperature at the time of each measurement (RMT). All correlations between SBST at the different measurement locations were strong and positive (P &lt; 0.0001; Table 1) with r values ranging from 0.64 (between vulva and the outer ear) to 0.89 (between vulva and ham). However, correlations between SBST and rectal temperature were relatively weak (range r = 0.14 to 0.18; P &lt; 0.0001). Rectal temperature was not correlated with RMT (r = 0.02; P = 0.12); correlations between RMT and SBST measurements were positive, with the strongest correlations being with the outer ear and the back of the head (r = 0.45 and 0.50, respectively; P &lt; 0.0001). In conclusion, this study suggests strong relationships between body surface temperatures at different locations but relatively weak relationships between these measurements and rectal temperature. In addition, these results suggest room temperature has a greater influence on body surface temperature than on rectal temperature.
PSIII-1 Preference Testing of Three Materials on Sows Prior to Farrowing
Authors: Underwood Lauren L , Patel Anjali , Garcia Arlene , Anderson Nichole C 
Published: October 2023

Abstract
Enrichment increases the ability of a sow to express innate behavior in a barren environment and can reduce aggressive behaviors, stress, and promote animal welfare. The objective of this study was to determine sow interaction with different types of enrichment materials provided before farrowing. A total of 11 sows were randomly assigned an enrichment combination of cardboard, paper, and banana leaves. Sows received two enrichment types, then enrichment was switched for the following day, until all combinations were completed. The enrichment material hung from 2x4 wood using ropes that hung the enrichment and chains placed in the ceiling of the farrowing room that held the 2x4 above the pen of each sow. Therefore, enrichment was placed within the nose reach of each sow. Enrichment material (113 g) was placed in the pens at 0700 h and left for 24 h each day before changing with another enrichment combination. Any enrichment material remaining was weighed. Final weight varied by treatment (P = 0.01), with sows consuming the least of cardboard (24.9 ± 10.0 g) and having an equal preference for banana leaves and paper (74.1 ± 10.0 g and 50.1 ± 11.3 g, respectively). Scan sampling was utilized to measure the frequency of interaction with the enrichments. The scans were designed with the first hour recording every minute a sow interacted with enrichment and the following 3 hours recording every 10 minutes. Continuous observation for the first hour of placement was performed for count data. Each time the sow made contact with the enrichment with her nose, the observation was recorded. The results of the behavior observation suggested that time in contact with enrichment did not differ (P &gt; 0.05), nor did the number of times the pig-initiated contact with enrichment (P &gt; 0.05). In conclusion, sow preference showed that bananas and papers were preferred over cardboard. However, sows will engage with any enrichment material provided before farrowing. Future research should be done to see if any environmental factors play a role in sow’s preference.
PSII-4 Effects of Rye or Triticale with or without Crimson Clover on Forage Yield and Quality, and Gestating Beef Female Performance
Authors: Ewing Kylie P , Shike Daniel W 
Published: October 2023

Abstract
The objective was to evaluate the effects of rye or triticale with or without crimson clover on forage yield and quality, and gestating beef female performance. In experiment 1, four cover crop treatments (trt) were randomly assigned to plot (0.49 ha/plot; 6 plots/trt) in a 2×2 factorial design. Treatments included cereal rye or triticale with or without crimson clover. Plots were seeded mid-September (rye or triticale: 101 kg/ha; crimson clover inclusion: 84 kg/ha cereal and 9 kg/ha legume). Treatments were mechanically harvested for haylage at the end of May. Plot yield was determined by total forage mass collection. Prior to ensiling, samples (12/plot) were composited within plot to evaluate forage quality. Data were analyzed using the MIXED procedure of SAS 9.4. Forage yield was greater (P &lt; 0.01) with inclusion of crimson clover than without (3,208 vs. 2,654 kg/ha, respectively). Forage yield was not different (P = 0.17) between cereal type. Crimson clover inclusion reduced dry matter (P &lt; 0.01). Between cereal types, rye had greater (P &lt; 0.01) DM than triticale. Crimson clover inclusion increased CP (P &lt; 0.01; 9.6% vs 8.6%, respectively). Neutral detergent fiber and acid detergent fiber were greater (P &lt; 0.01) in rye compared with triticale. In experiment 2, spring-calving cows and heifers (n = 105; BW = 633 ± 100 kg; gestation = 198 ± 17 days) were randomly assigned to 1 of 5 treatment diets in a 42-day feeding trial to determine feed value of cover crops. Four diets were based on cover crops (60% inclusion) from experiment one with an additional control diet (CON; 85% alfalfa hay and 15% modified wet distillers grains). All treatments were limit fed (11.3 BW∙animal-1∙d-1/). Body weights (BW) and body condition scores (BCS) were assessed at trial initiation and conclusion. Data were analyzed using the MIXED procedure of SAS 9.4. Female final BW and BW change were greater (P &lt; 0.01) in cover crop treatments compared with CON. Body weight change did not differ (P ≥ 0.21) between cereal type or with inclusion of crimson clover. Initial BCS was not different (P ≥ 0.47); however, final BCS was greater (P &lt; 0.01) in cover crop treatments compared with CON. Crimson clover inclusion increased (P = 0.02) BCS change. In summary, inclusion of crimson clover increased forage yield, CP, and female BCS. Although rye cover crops had decreased quality prior to ensiling, there were no differences in cow performance compared with triticale. Substituting cover crops for alfalfa hay resulted in increased cow BW and BCS, suggesting the cover crops evaluated have greater feed value than alfalfa hay.
PSII-11 Supplementation of Non-Protein Nitrogen to a Nitrogen-Deficient Diet Increases Lysine Requirement for Nitrogen-Retention
Authors: Buchinski Miranda , Camire Carley M , Panisson Josiane C , Shoveller Anna K , Columbus Daniel A 
Published: October 2023

Abstract
Low protein (LP) diets have improved nutrient utilization while maintaining animal performance. Regardless, these diets may be limiting in nitrogen (N) content to meet non-essential amino acid (NEAA) requirements, potentially altering essential amino acid (EAA) utilization and requirements. Inclusion of a non-protein nitrogen (NPN) may be beneficial for improving utilization of EAA in LP diets. The objective of this study was to determine the impact of NPN in an N-deficient diet, as indicated by EAA-N:total N ratio (EAA-N:TN) on the Lys requirement for nitrogen retention (NR). A total of 90 growing barrows (initial BW of 20.4 ± 0.46 kg) were randomly assigned to 1 of 10 dietary treatments (n = 9 pigs/treatment) in 9 blocks in a 2 × 5 factorial design. Diets contained no ammonium phosphate (NAP; EAA-N:TN of 0.56) or were supplemented with 1.7% ammonium phosphate (AP; EAA-N:TN of 0.50) with graded levels of dietary lysine [Lys; 0.8%, 0.9%, 1.0%, 1.1% and 1.2% standardized ileal digestible (SID)]. Diets were corn and soybean meal-based and formulated to meet or exceed nutrient requirements according to NRC (2012). Diets were fed at 2.8 × maintenance metabolizable energy requirements in two equal meals per day. A 4-d nitrogen balance collection period was conducted following a 7-d dietary adaptation period. Urine was collected quantitatively, and fresh fecal samples were obtained daily. Nitrogen retention was determined as the difference between N intake and output (fecal and urinary). Linear breakpoint modeling with PROC NLIN was used to estimate Lys requirement. Nitrogen retention was optimized at 1.00% SID Lys (15.6 g/d NR; R2 = 0.68) in pigs fed NAP diets and 1.09% SID Lys (16.4 g/d NR; R2 = 0.61) in pigs fed AP diets. Overall, deficient dietary N reduced NR and Lys requirement, which were increased with NPN supplementation.
PSIII-17 Evaluation of Cheese co-Products in Comparison with Specialty Proteins on Growth Performance of Weaned Pigs
Authors: Hung Yuan-Tai , Fruge Emily , Reznik Gary , Droegmiller Jon , Lutz Trevor 
Published: October 2023

Abstract
Plant protein, animal protein, and milk protein are common protein sources in nursery diets. To ensure growth performance of pigs and profitability of feeding programs, specialty protein ingredients are strategically selected by nutritionists primarily based on energy value, digestible amino acid (AA) content, nutrient variability, and cost. Indeed, standardized ileal digestibility of AA and metabolizable energy are greater in cheese co-products than fishmeal or enzymatic-treated soybean meal (ESBM). Therefore, two experiments were conducted to determine if replacing specialty protein sources with a novel cheese co-product in nursery diets could improve growth variables and profitability. In experiment 1, a total of 540 pigs (PIC 800 x Camborough; 7.1 kg) were blocked by body weight and allotted to 3 treatments, 10 replicates per treatment, and 18 pigs per pen. The three dietary treatments were a control diet containing ESBM and microbial biomass (MB) or 5% of cheese co-product from manufacturer A or B added to the basal diet with replacement of ESBM and MB. In experiment 2, a total of 1,080 pigs (PIC 800 x Fast 276; 6.7 kg) were blocked by body weight and allotted to 4 treatments, 15 replicates per treatment, and 18 pigs per pen. The four treatments were a control diet with ESBM and MB, control with 5% of cheese co-product by replacing ESBM; control with 0.5% of functional protein; control with 4% of bovine plasma. All experimental diets were formulated on an equal SID Lys basis and a common diet was provided after approximately 3 weeks post-weaning. Data were analyzed using generalized linear mixed models with treatment as a fixed effect and block as a random effect. Results from experiment 1 showed that pigs fed cheese co-products regardless of the source had greater ADG (P &lt; 0.05) by 4.4% than those fed control. Results from experiment 2 showed that pigs fed cheese co-product had greater (P &lt; 0.05) intake in week 2 and week 3, which resulted in an overall increase in feed intake by 7.5% compared with those fed functional protein. However, there were no difference in overall gain, ADG, and feed efficiency during the entire 40-day study. Economic assessment showed that pigs fed cheese co-products had better income over feed cost than control in experiment 1 and had an equivalent income over feed cost compared with control in experiment 2. These results suggest that the novel cheese co-products can effectively substitute for ESBM and other specialty proteins in nursery diets and provide some flexibility to substitute expensive protein sources without jeopardizing growth and profitability.
PSIII-9 Effects of a Specialty Energy Source on Growth Performance and Fecal Microbiota in Nursery Pigs
Authors: Wang Tingting , Tsai Tsungcheng Cheng , Maxwell Charles V , Frank Jason 
Published: October 2023

Abstract
The objective of this study was to evaluate the effects of a specialty energy source, which comprises of 28% fat and 55% corn-syrup solids (Solu-E; International Ingredient Corporation, Fenton, MO) on growth performance and fecal microbiota population of nursery pigs. Two hundred and eighty-eight (initial body weight = 5.91 kg) mixed gender pigs were assigned to 4 dietary treatments in a randomized complete block design with body weight as a blocking factor. There were 12 replicate pens per treatment with 6 pigs per pen. Experimental diets were fed in the first 2 phases (d 0 to 21) in pellet form followed by a common phase 3 (d 21 to 42) diet in mash form. Four levels of Solu-E (0%, 4%, 8%, and 12%) were used to formulate 4 dietary treatments for phase 1 (d 0 to 7) and phase 2 (d 7 to 21) at the expense of poultry fat and corn to be isocaloric. There was no difference in average daily feed intake among four treatments during this experiment (Table 1). Average daily gain (ADG) in phase 1 linearly increased (P &lt; 0.05) with increasing inclusion level of Solu-E in experimental diets (from 0.092 to 0.113 kg/d). Pigs fed diets with 8% Solu-E had greater (P &lt; 0.05) ADG and feed efficiency compared with 4% and 12% Solu-E in phase 2, but no difference from 0% Solu-E. Pigs fed diets with 8% Solu-E had greater (P &lt; 0.05) ADG and feed efficiency compared with 0%, 4%, and 12% Solu-E from d 0 to 21, which is consistent with the heavier (P &lt; 0.05) body weight after 21 days of feeding 8% Solu-E diets than other diets. Real-time PCR assay for Enterotoxigenic Escherichia coli, Clostridium perfringens, Salmonella spp., and Lawsonia intracellularis were performed on pooled fecal samples from each treatment collected on d 7. The effect of Solu-E on fecal microbiota was not observed in this study as the PCR assay results were negative for all four treatments. In summary, 8% Solu-E can be used as an excellent energy source to enhance growth performance in the first three weeks of nursery.
PSV-20 Evaluation of Dietary Fiber Inclusion on Semen Production and Semen Characteristics in Mature AI Boars
Authors: Scaff Taw , Richert Brian T , Stewart Kara R , Denton Alicia N , Hedrick Hailey M 
Published: October 2023

Abstract
This experiment was conducted at Purdue University swine research and education center. The objective of this study was to determine if the inclusion of fiber (8.48% vs 14.85% NDF) in the diet of boars influenced semen characteristics, concentration, motility, and morphology. Twenty-seven boars from two age groups (7 months and 18 months) and two genetic lines (maternal and terminal) were utilized in this study over a 12-week period with one week before the study data serving as a baseline for statistical analysis. Boars were blocked by age and breed and randomly allotted to receive 2.72 kg/d of corn-soybean meal diet (CON, n=13) formulated to meet NRC (2012) requirements or an isocaloric (estimated ME = 3,285 Kcal/kg) corn-soybean meal diet with 14.3% soyhulls and supplemental choice white grease (4.65%) inclusions (FIBER, n = 14). Semen was collected once per week per boar using the gloved hand method. While at the farm 3 mL of semen was mixed with 27 mL of extender (Androhep Plus, Minitube) to make a 1:10 dilution. The semen was then driven approximately 19 km to Purdue University where concentration (Nucleocounter SP-100), motility (Computer Assisted Sperm Analysis, CASA, CEROS II), and sperm cell morphology were analyzed. Statistical analyses were conducted using PROC MIXED in SAS 9.4 (Cary, NC), with the main effects of diet, age, and breed with week as a repeated measure. Significance was determined at P &lt; 0.05 and a trend was observed at 0.05 &lt; P ≤ 0.10. Fiber in the diet did not affect any semen characteristics throughout the study. However, the percent morphologically normal sperm was greater in young boars compared with the older boars (80.6% vs 74.1%, P = 0.0143) resulting from fewer head/tail abnormalities (1.8% vs 2.4%, P = 0.0249) and distal droplets (7.2% vs 9.6%, P = 0.0508). Maternal boars had fewer proximal droplets (6.9% vs 9.9%, P = 0.0325) and tended to have fewer head/tail abnormalities (1.8% vs 2.3%, P = 0.0800). Terminal boars tended to have fewer distal midpiece reflex abnormalities than maternal boars (3.3% vs 4.8%, P = 0.0948). Overall feeding boars a high fiber diet had no effect on semen characteristics; however there were effects of age and breed of boar on semen characteristics. Future research is needed with varying levels and types of fiber inclusion in the diet along with differing ages and breeds of boars to better understand how fiber may impact semen characteristics.
PSVI-18 Effects of Vitamin U Supplementation on Growth Performance, Carcass and Gastric Keratinization Scores in Aspirin-Induced Gastric Ulcer Finishing Pigs
Authors: Jang Se Yeon , Yu Sun Gu , Lim Chai Bin , Shanchuan Cao , Kim In Ho 
Published: October 2023

Abstract
Oesophagogastric ulceration is a common disease in pigs. It is almost exclusively confined to the non-glandular pars-oesophagea. The prevalence of this gastric ulcer has been raised globally. Vitamins and trace elements play important roles in different physiological processes in pigs. Requirements of these elements will depend on ages, physiological statuses and feeds. Hence, there were only limited studies conducted on Methyl methionine sulfonium chloride (also known as Vitamin U), particularly in barrows and gilts. Thus, we investigated the beneficial effects of Vitamin U supplementation on the gastric ulcer problem of finishing pigs. One hundred-forty [(Landrace × Yorkshire) × Duroc] barrows and gilts (86.5 ± 1.5 kg) were used in a 28-d experiment in a randomized complete blocked design and assigned to TRT1 (basal diet); TRT2- (basal diet + stress), TRT3 (basal diet + 0.05 % Vitamin U), TRT4 (basal diet + aspirin 15g (3 days) + stress), and TRT5 (basal diet + aspirin 15g (3 days) + stress + 0.1 Vitamin U ), respectively. The active ingredients of Vitamin U: 50mg/g, MgO 60mg/g, Na2CO3 70mg/g. TRT 5 group pigs showed a significant increase (P &lt; 0.05) in final body weight and average daily gain (ADG) compared with other groups. However, there was no significant difference in body condition scores between treatment groups (P &gt; 0.05). Stomach keratinization scores were significantly greater in TRT2 and 4 than in TRT 3 (P &lt; 0.05). In summary, the addition of Vitamin U (stayvill) to the diet improved the degree of gastric ulcer to a certain extent and effectively improve growth performance in finishing pigs.
PSIV-8 The Effects of Quercetin Supplementation During Oocyte Maturation on the in Vitro Production of Pig Embryos
Authors: Nau Cameron , Throop Makayla , Christy Alayna , Whitaker Brian D 
Published: October 2023

Abstract
Oocyte viability can be reduced during in vitro maturation due to oxidative stress and a lack of antioxidant presence in the environment. Additionally, oxidative stress is a culprit in the relatively low success rate of in vitro fertilization (IVF) in pigs. Quercetin is a natural antioxidant dietary flavonoid that has the ability to suppress free radical formation. The purpose of this study was to determine the effects of the quercetin supplementation during oocyte maturation on IVF embryonic development success rates in pigs. Oocytes were matured for 40 to 44 h supplemented with quercetin (0, 0.25, 0.5, 0.75 mM; Sigma-Aldrich Co.) then fertilized using frozen-thawed boar semen (1.0 x 105 sperm cells/mL) and co-incubated for 6-8 h followed by embryo culture for 144 h. After maturation, oocytes were evaluated for cumulus cell expansion (n = 300, r = 6) under a stereomicroscope (scale 0 to 4) then denuded to determine reactive oxygen species (ROS) production (n = 135) by measuring the fluorescent intensity from the oxidation of 2’,7’-dichlorodihydrofluorescein diacetate. After IVF, zygotes were fixed, permeabilized, and stained with bisBenzimide H 33342 trihydrochloride and evaluated for penetration, polyspermy, and pronucleus formation rates (n = 480) and embryos were evaluated for cleavage and blastocyst formation (n = 599) at 48 h and 144 h post-IVF, respectively. Cumulus cell expansion score percentages were determined and analyzed using the Chi-squared test. Fluorescent intensities of oocytes were analyzed using a GLM and compared using LSD after adjusting the control group value to 1. The IVF and embryo development data were reported as the percent observed/drop and mean percentages, and were analyzed using GLM. Means were analyzed using Tukey’s test. The average cumulus cell expansion post-maturation when no quercetin was supplemented was 2.78 ± 0.15. As quercetin supplementation increased, cumulus cell expansion decreased (1.33 ± 0.11 for 0.75 mM quercetin). Supplementation of 0.25 mM quercetin decreased (P &lt; 0.05) ROS levels (0.52 ± 0.12) but 0.75 mM quercetin increased (P &lt; 0.05) ROS levels (1.73 ± 0.11) compared with no quercetin. Supplementation of quercetin decreased (P &lt; 0.05) oocyte penetration rates, increased (P &lt; 0.05) polyspermic oocytes and pronuclear formation. Quercetin supplementation during maturation also reduced embryonic development success through decrease (P &lt; 0.05) of cleavage and blastocyst formation rates compared with no quercetin (78.69 ± 0.02 and 31.18 ± 0.03, respectively). It appears that quercetin supplementation to the oocyte maturation media in concentrations greater than 0.25 mM are detrimental during in vitro production of pig embryos. Supplementation of 0.25 mM quercetin appears to not be different to supplementing quercetin to the maturation media except for reduction in ROS concentrations in the oocyte.