bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro response of MC3T3‐E1 preosteoblasts within three‐dimensional apatite‐coated PLGA scaffolds
Authors: Chou Yu‐Fen , Dunn James C. Y. , Wu Benjamin M. 
Published: July 2005

AbstractBiomimetic apatites have been reported to promote osteogenic activities in numerous in vivo and in vitro models, but the precise mechanism by which the apatite microenvironment promotes such activities is not well understood. Such mechanistic studies require reproducible model systems that are relevant to tissue engineering practices. Although two‐dimensional (2D) apatite‐coated polystyrene culture dishes provide practicality and reproducibility, they do not simulate the effects of the three‐dimensional (3D) microenvironment and degrading polymeric substrates. A simple 3D model system to address these relevant effects, and its utilization in the investigation of apatite‐promoted osteoblastic differentiation in vitro is reported in this paper. Apatite coating was achieved by sequentially immersing poly(lactide‐co‐glycolide) (PLGA) scaffolds into different simulated body fluids (SBF). SEM, EDX, FTIR, TEM electron diffraction confirmed the apatite coating to comprise of calcium‐deficient carbonated hydroxyapatite crystals. While both apatite‐coated and noncoated PLGA scaffolds supported MC3T3‐E1 attachment, spreading, and proliferation, significant differences in osteoblastic differentiation were observed. Relative to noncoated controls, quantitative real‐time PCR revealed significant apatite‐associated suppression of alkaline phosphatase (ALP), early upregulation of osteopontin (OPN) at 3 days, and upregulation of osteocalcin (OCN) and bone sialoprotein (BSP) at 4 weeks. In summary, apatite‐promoted osteoblastic differentiation can be observed in a 3D model system that is relevant to tissue engineering. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005
Functional transfer of eukaryotic expression plasmids to mammalian cells by Listeria monocytogenes: a mechanistic approach
Authors: Zelmer Andrea , Krusch Stefan , Koschinski Andreas , Rohde Manfred , Repp Holger , Chakraborty Trinad , Weiss Siegfried 
Published: July 2005

AbstractBackgroundCystic fibrosis (CF) is one of the most common monogenic disorders in the caucasian population. Gene therapy for CF is principally feasible and bacterial transfer systems might provide novel possibilities for therapy. However, transfection efficiencies are low and need to be improved. Thus, more detailed understanding of the DNA transfer mechanism is necessary to systematically eliminate these restrictions.MethodsFunctional transfer of GFP‐CFTR (cystic fibrosis transmembrane conductance regulator) to eukaryotic cells using attenuated Listeria monocytogenes mediated gene transfer (bacteriofection) was shown by fluorescent microscopy, flow cytometry, immunoblotting and whole cell patch clamping. The characteristics of plasmid transfer were studied by use of electron and fluorescence microscopy, flow cytometry and Southern blotting. Polymerase chain reaction (PCR) was used to screen the genome of bacteriofected cells for cotransfer of chromosomal bacterial DNA.ResultsCorrect intracellular localization and functionality of the GFP‐CFTR fusion protein after bacteriofection was shown. Efficient bacterial lysis and release of bacterial content was demonstrated using antibiotics to kill intracellular bacteria. Although only low transfection rates were observed, high numbers of transferred plasmids were detected in host cells under these conditions. However, they were associated with high molecular weight entities and not available to cytosolic transcription. Cotransfer of bacterial genomic DNA was observed in transfectants but occurred at low frequencies.ConclusionsIn this work we demonstrate that low rates of bacteria‐mediated transfection are not due to poor invasion of bacteria, insufficient bacterial lysis, or plasmid DNA degradation. Our data suggest that the transferred plasmid DNA is associated with higher macromolecular structures inhibiting nuclear transport and transgene transcription. Copyright © 2005 John Wiley & Sons, Ltd.
Laparoscopy‐assisted distal gastrectomy with systemic lymph node dissection: A phase II study following the learning curve
Authors: Fujiwara Michitaka , Kodera Yasuhiro , Miura Shinichi , Kanyama Yasuaki , Yokoyama Hiroyuki , Ohashi Norifumi , Hibi Kenji , Ito Katsuki , Akiyama Seiji , Nakao Akimasa 
Published: July 2005

AbstractBackground and ObjectivesA preliminary study on the use of laparoscopy‐assisted approach to treat gastric carcinoma resulted in higher morbidity.Study DesignA prospective phase II study of laparoscopy‐assisted distal gastrectomy (LADG) was performed for patients with preoperative diagnosis of T1 N0 stage cancer located in the lower or middle‐third stomach. Bleeding amount, operating time, mortality, morbidity, and the number of lymph node retrieval were recorded and compared with the preliminary series reported previously by the same authors.ResultsBetween 2000 and 2002, 47 patients were accrued. The mean blood loss and postoperative hospital stay were significantly decreased compared with the previous series, whereas the operating time was not. There were no in‐hospital deaths, with the incidence of anastomotic leakage significantly decreased. All patients remain disease‐free to date.ConclusionsLADG can be performed safely and morbidity, no longer, is a drawback by experienced hands that have reached plateau of the learning curve, although it remains a time‐consuming procedure. Its application to gastric cancer surgery is feasible for early stage cancer, and its applicability to the treatment of T2 stage cancer will be the next issue to be explored. J. Surg. Oncol. 2005;91:26–32. © 2005 Wiley‐Liss, Inc.
Retroperitoneal sarcoma: 25 years of experience with aggressive surgical treatment at the Institute of Oncology, Ljubljana
Authors: Erzen Darja , Sencar Mojca , Novak Janez 
Published: July 2005

AbstractBackground and ObjectivesRetroperitoneal sarcomas are rare malignant tumors with an aggressive course of disease and high local recurrence rate. Local disease is the main cause of death. A retrospective study was undertaken to evaluate the results of aggressive surgical treatment in a series of patients of primary and recurrent retroperitoneal sarcomas.Patients and methodsFrom 1975 to 2000, 155 patients with retroperitoneal tumors were operated on at the Institute of Oncology, Ljubljana. Patients with fibromatosis and children less than 16 years were excluded from further calculation. Out of the rest 139 patients, 102 patients had localized retroperitoneal sarcoma, and 37 patients had retroperitoneal sarcoma with distant metastases Leiomyosarcoma was the most common type of histology, followed by liposarcoma and schwannoma. Their referral status was as follow: 56 had primary sarcoma, 20 residual sarcoma after operation elsewhere and 26 already recurrent sarcoma. Our treatment approach was aggressive. We removed surgically primary tumor, recurrent sarcoma and metastases, whenever possible. To this end, we performed, 235 operations for retroperitoneal sarcomas on 139 patients (up to 8 operations on 1 patient).ResultsComplete resection was done in 97of 102 patients with localized sarcoma (resectability rate 95%); in 55 patients, R0 resection was made, in 42 patients, R1 resection, in 3 patients, debulking procedure, and in 2 patients biopsy alone. In 118, patients at least one organ was resected completely or partially en bloc with tumor. The 5‐, 10‐, and 15‐year survivals of patients with localized sarcoma were 52%, 36%, and 22%, respectively. The 5‐year survival of patients with metastases was 12% (P = 0.0002), the 5‐ and 10‐year survival rates of patients with R0 resection were 75% and 64%, respectively, and of those with R1 resection, 25% and 8%, respectively (0 < 0.00001). High tumor grade was associated with poor survival. The 5‐year local recurrence rate of patients with primary sarcoma and of those with locally recurrent sarcoma was 37% and 71%, respectively (P = 0.04). After the first local recurrence, the 5‐ and 10‐year survival rates were 42% and 26%, after the second local recurrence 45% and 22%, after the third local recurrence 43% and 11%, and after the fourth local recurrence 51% and 17%, respectively.ConclusionComplete surgical resection without microscopic residuum and contamination is likely to offer the best chances for long‐term survival. Until there are no other treatment modalities, aggressive surgery for recurrent sarcoma is recommended. J. Surg. Oncol. 2005;91:1–9. © 2005 Wiley‐Liss, Inc.
Inguinal canal as an anatomic sanctuary site of relapse in peritoneal carcinomatosis previously treated with intraperitoneal chemotherapy
Authors: Liberale Gabriel , Elias Dominique , Sideris Lucas , Lasser Philippe , Malka David , Sabourin Jean‐Christophe , Pocard Marc 
Published: July 2005

AbstractEarly postoperative intraperitoneal chemotherapy (EPIC) and intraoperative peritoneal hypertermic chemotherapy (IPHC) are used in addition with cytoreductive surgery to treat with curative intent peritoneal carcinomatosis arising from colorectal adenocarcinomas. Three patients with such a disease were treated with perioperative intraperitoneal chemotherapy in addition to cytoreductive surgery and presented isolated local recurrence located in the inguinal canal (round ligament in two and spermatic cord in one). All these patients were treated by local surgical excision. No patient showed evidence of intra‐abdominal recurrence at the last follow‐up, but one developed pulmonary metastasis. When communicating with the peritoneal cavity, the inguinal canal may act as a sanctuary site for peritoneal carcinomatosis, since it is not totaly soaked by the intraperitoneal chemotherapy solution. A local recurrence is thus possible. New clinical presentations such as this one have first to be described in order to improve patient follow‐up. J. Surg. Oncol. 2005;91:73–76. © 2005 Wiley‐Liss, Inc.
Comparison of laparoscopy‐assisted by conventional open distal gastrectomy and extraperigastric lymph node dissection in early gastric cancer
Authors: Kim Min‐Chan , Kim Ki‐Han , Kim Hyung‐Ho , Jung Ghap‐Joong 
Published: July 2005

AbstractBackground and ObjectivesLaparoscopy‐assisted gastrectomy with lymph node dissection for gastric cancer is considered technically more complicated than the open method. Moreover, the safety and efficacy of laparoscopy‐assisted distal gastrectomy (LADG) with extraperigastric lymph node dissection in patients with gastric cancer have not been established yet. To evaluate short‐term surgical validity, surgical outcome of the laparoscopy‐assisted distal gastrectomy (LADG) with extraperigastric lymph node dissection was compared with that of the conventional open distal gastrectomy (CODG) in patients with early gastric cancer.MethodsOne hundred and forty‐seven patients with early gastric cancer received radical distal gastrectomy during 2002 and 2003, where LADG was undergone in 71 patients. The clinicopathologic characteristics, postoperative outcomes and courses, and postoperative morbidities and mortalities were compared between the two groups. Data were retrieved from the stomach cancer database at Dong‐A University Medical center.ResultsBaseline characteristics, including sex, age, body mass index (BMI), American Society of Anesthesiology (ASA) class, tumor size, T stage, and lymph node metastasis were similar between the two groups. No significant differences were found between these groups in terms of the number of retrieved lymph nodes with respect to D1 + α (D1 + no. 7) and D1 + β (D1 + no. 7, 8a, and 9) lymphadenectomy. In the LADG group, wound size was smaller (P < 0.0001), but operation time was longer (P = 0.0001) than in the CODG group. Perioperative recovery was faster in the LADG group than in the CODG group, as reflected by a shorter hospital stay (P = 0.0176) and less times of additional analgesics (P = 0.0370). Serum albumin level in LADG was higher (P = 0.0002) on day 7 than that in CODG, and the leukocyte count in LADG lower (P = 0.0445) on day 1 than that in CODG. Postoperative morbidities and mortalities were not significantly different between the two groups.ConclusionsOur data confirmed that LADG with extraperigastric (no. 7, 8, and 9) lymph node dissection proved to be feasible and acceptable surgical technique for early gastric cancer. At least taking a surgical point of view, LADG with extraperigastric lymph node dissection is suggested to be a preferred surgical option for patients with early gastric cancer. Its oncologic validity awaits larger and prospective multicenter trials. J. Surg. Oncol. 2005;91:90–94. © 2005 Wiley‐Liss, Inc.
The role of glial cell line‐derived neurotrophic factor (GDNF) and integrins for invasion and metastasis in human pancreatic cancer cells
Authors: Funahashi Hitoshi , Okada Yuji , Sawai Hirozumi , Takahashi Hiroki , Matsuo Yoichi , Takeyama Hiromitsu , Manabe Tadao 
Published: July 2005

AbstractBackground and ObjectivesIt is generally accepted that the malignancy of pancreatic cancer is dependent upon the extent of invasion as well as metastasis. However, the factors and mechanisms are incompletely understood. We investigated whether glial cell lined‐derived neurotrophic factor (GDNF) enhances the invasive and adhesive behaviors of pancreatic cancer cells by altering of the expression of integrins.MethodsThe expression of the GDNF receptor in pancreatic cancer cell lines (SW1990 and Capan‐2) was confirmed by RT‐PCR. Then we determined the expression of integrin subunits and the alteration of their expression by GDNF using flow‐cytometric analysis and a cellular enzyme‐linked immunosorbent assay (CELISA). Adhesion and invasion assay were performed to investigate whether increased integrin expression affected the interaction between cancer cells and ECM proteins.ResultsThe GDNF receptor subunits were expressed in pancreatic cancer cells. GDNF enhanced the expression of some of the integrin subunits and increased their adhesive and invasive abilities. The enhanced expression and associated increase in adhesive and invasive abilities were inhibited by blocking the GDNF receptor or the integrin β1 subunit.ConclusionThe enhancement of integrin expression by GDNF signaling through the GDNF receptor strongly influences invasion and adhesion to ECM proteins by pancreatic cancer cells. J. Surg. Oncol. 2005;91:77–83. © 2005 Wiley‐Liss, Inc.
Predictive value of p53 and 14‐3‐3σ for the effect of chemoradiation therapy on esophageal squamous cell carcinoma
Authors: Okumura Hiroshi , Natsugoe Shoji , Matsumoto Masataka , Yokomakura Naoya , Uchikado Yasuto , Takatori Hiroyuki , Ishigami Sumiya , Takao Sonshin , Aikou Takashi 
Published: July 2005

AbstractBackground and ObjectivesThe p53 family regulates cell‐cycle arrest, triggers apoptosis, repairs DNA damage caused by various genotoxic stresses, and protects cells from death upon irradiation. The purpose of the present study was to examine the expressions of p53 and one of the p53 family proteins, 14‐3‐3σ, in biopsy specimens and to predict the clinical and histological responses to chemoradiation therapy (CRT) in patients with esophageal squamous cell carcinoma (ESCC).MethodsWe investigated with the relationship between p53 and 14‐3‐3σ expressions in biopsy specimens obtained from 62 patients with ESCC and analyzed these patients' clinical and histological responses to CRT. Chemoradiation therapy consisted of 5‐fluorouracil plus cisplatin and 40 Gy of radiation.ResultsFollowing CRT, 71.0% of patients showed a positive clinical response and 52.8% showed a positive histological response. The rate of positive expression was 43.5% for p53 and 58.1% for 14‐3‐3σ. Statistically significant correlations were found between p53 expression and clinical response to CRT (P = 0.001) and histological response to CRT (P = 0.041), and between 14‐3‐3σ expression and histological response to CRT (P = 0.01). Furthermore, in p53‐positive tumors, CRT was more effective in tumors with 14‐3‐3σ‐positive expressions than those with 14‐3‐3σ‐negative expressions (P = 0.037). The survival rate of the patients with 14‐3‐3σ‐positive tumors was better than those with 14‐3‐3σ‐negative tumors in patients with p53‐positive tumors (P = 0.047).ConclusionsWe demonstrated that p53‐negative or 14‐3‐3σ‐positive expressions were closely related to the response to CRT. It is clinically useful to examine the expression of these genes in biopsy specimens for predicting the CRT outcomes in patients with ESCC. J. Surg. Oncol. 2005;91:84–89. © 2005 Wiley‐Liss, Inc.
Butyrate regulates the expression of c‐Src and focal adhesion kinase and inhibits cell invasion of human colon cancer cells
Authors: Lee Jenq‐Chang , Maa Ming‐Chei , Yu Hsiu‐Shan , Wang Jung‐Hui , Yen Chia‐Kuang , Wang Shan‐Tair , Chen Yen‐Jen , Liu Yuan , Jin Ying‐Tai , Leu Tzeng‐Horng 
Published: July 2005

AbstractEpidemiological studies indicate that dietary fiber‐derived fermentation products such as butyrate can prevent colon cancer development. To further dissect the role of butyrate in anticarcinogenesis, its effect on cellular growth and invasion as well as the expression of c‐Src and FAK, two mutually interactive nonreceptor tyrosine kinases, in three different human colon cancer cell lines (Caco‐2, SW480, and SW620) were investigated. In addition to growth inhibition, butyrate treatment results in a significant downregulation of c‐Src and FAK in human colon cancer cells, which can be attributable to their reduced transcripts and implicates the participation of a butyrate‐sensitive pathway in modulating their expression. Concurrent to butyrate‐reduced c‐Src and FAK expression is the decrease of FAK Tyr‐decrease 397 phosphorylation. Besides, butyrate also abolished the secretion of MMP‐2 and MMP‐9. And these butyrate‐mediated effects severely impaired invasion of SW620 cells through Matrigel in vitro. Interestingly, in situ parallel enhancement of c‐Src and FAK was also observed in human colorectal tumor specimens. These results imply that by virtue of suppression of c‐Src and FAK along with other butyrate targets in colonocytes, butyrate could effectively inhibit tumor growth and invasion. © 2005 Wiley‐Liss, Inc.
Transcultural comparison of psychogenic movement disorders
Authors: Cubo Esther , Hinson Vanessa K. , Goetz Christopher G. , Garcia Ruiz Pedro , Garcia de Yebenes Justo , Marti María José , Rodriguez Oroz Maria Cruz , Linazasoro Gurutz , Chacón José , Vázquez Antonio , López del Val Javier , Leurgans Sue , Wuu Joanne 
Published: July 2005

AbstractPrompted by the lack of cross‐cultural comparative data, and because a better understanding in the different clinical presentations of psychogenic movement disorders (PMDs) is relevant to neurological assessment and interventions, we compared the phenomenology, anatomical distribution, and functional impairment of PMDs in the United States and Spain. Consecutive patients diagnosed with PMD by a movement disorder specialist from one US site and from eight Spanish university centers were included in the study. The two groups were similar in their movement types, anatomical distribution, and functional impairment. PMDs were more prevalent in women than in men and were most common in upper and lower extremities. Gait and speech dysfunctions were distributed similarly in both countries. We found action tremor to be the most frequent PMD in both countries. © 2005 Movement Disorder Society
No going home for hospitalized Huntington's disease patients
Authors: Dubinsky Richard M. 
Published: July 2005

AbstractLittle is known as to what happens to people with Huntington's disease (HD) at the end of life. Exploratory analysis was performed for all admissions of people with HD in the Nationwide Inpatient Sample database, a 20% stratified sample of all US hospitalizations, from 1996 to 2002. Using the principal diagnosis and procedure, admission cohorts were determined. The common HD‐associated reasons for admission were pneumonia (including aspiration, 22%), psychiatric (21.5%), debilitation (hypovolemia, nutritional deficiencies, and decubitus ulcers, 15.5%), trauma (5.7%), and nonpulmonary infections (urinary tract infections and sepsis, 11.%). Twenty‐two percent were admitted for medical problems unrelated to HD. Emergency departments were the most frequent source of admission (60%), most had Medicare or Medicaid as the expected payer (80.6%), 54.4% of all admissions were discharged to long‐term care facilities (LTCFs), and 3.7% died. The highest mortality was seen in the pneumonia (7.68%) and sepsis (7.39%) cohorts. Of the elective admissions, between 49.71% and 60.31% were discharged to LTCFs, representing new LTCF admissions. Based on this exploratory analysis, hospitalized HD patients are admitted late in the course of their illness, are severely disabled, and are commonly discharged to LTCFs. With 60.31% of the discharges representing new LTCF admissions, this finding shows that there is no going home from the hospital for people with HD. © 2005 Movement Disorder Society
Defining the breakpoints of the quakingviable mouse mutation reveals a duplication from a Parkin intron
Authors: Dapper Jason D. , Justice Monica J. 
Published: July 2005

AbstractThe quakingviable (qkv) mutant mouse shows a recessive neurological phenotype that includes central nervous system (CNS) dysmyelination, seizures, and tremor associated with voluntary movement. The molecular defect of qkv has been previously reported to be a spontaneous ∼1 megabase (Mb) deletion in the proximal region of mouse chromosome 17 that occurred in the DBA mouse strain more than four decades ago. The mutation has recently been shown to affect three genes in the region: Quaking (qk), Parkin‐coregulated gene (Pacrg), and Parkin. Here we determine the exact deletion breakpoints and demonstrate that the mutation is not just comprised of a ∼1.1 Mb deletion, but also harbors a small 163 bp duplication fragment between the deletion breakpoints. Although the distal deletion breakpoint is within the fifth intron of the mouse Parkin gene, the duplicated sequence is derived from the sixth Parkin intron and shows positive transcriptional activity on a reporter gene in vitro. This complexity provides insight into a well‐studied neurological mutant and may have a role in affecting the phenotype observed. © 2005 Movement Disorder Society
Poly(caprolactone‐co‐lactide)/perfluoropolyether block copolymers: Synthesis, thermal, and surface characterization
Authors: Bongiovanni Roberta , Malucelli Giulio , Messori Massimo , Pilati Francesco , Priola Aldo , Tonelli Claudio , Toselli Maurizio 
Published: July 2005

AbstractPoly[(caprolactone‐co‐lactide)‐b‐perfluoropolyether‐b‐(caprolactone‐co‐lactide)] copolymers (TXCLLA) were prepared by ring‐opening polymerization of D,L‐dilactide (LA2) and caprolactone (CL) in the presence of α,ω‐hydroxy terminated perfluoropolyether (Fomblin Z‐DOL TX) as macroinitiator and tin(II) 2‐ethylexanoate as catalyst. 1H NMR analysis showed that LA2 is initially incorporated into the copolymer preferentially with respect to CL. A blocky structure of the polyester segment was also indicated by the sequence distribution analysis of the monomeric units. Differential scanning calorimetry analysis showed the compatibility between poly(lactide) (PLA) and poly(caprolactone) (PCL) blocks inside the amorphous phase with glass‐transition temperature values increasing from −60 to −15 °C by increasing the PLA content. Copolymers with high average length of CL blocks were semicrystalline with a melting temperature ranging from +35 to +47 °C. Surface analysis showed a high surface activity of TXCLLA copolymers with values of surface tension independent from the PLA/PCL content and very close to those of pure TX. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 3588–3599, 2005