bluuebunny/crossref_metadata_2025_split huggingface dataset:
HYQTD Drug-containing Serum Alleviates H2O2-induced Endothelial Oxidative Damage by Increasing Mitochondrial ATP Synthesis and Inhibiting ROS
Authors: Wu Jin , Chen Lijuan , Du Ying , Leng Xue , Yuan Dongchao , Yang Mingqian , Zhao Yeyu , Lv Bin , Jia Lianqun 
Published: January 2025

Background:
Atherosclerosis (AS) is caused by the endothelium injury associated
with oxidative stress. Previous studies have shown that the Phlegm-Eliminating and Stasis-
Transforming Decoction (Huayu Qutan Decoction, HYQTD) has mitochondrial protective function.
The objective of this research was to explore how HYQTD drug-containing serum
(HYQTD-DS) could potentially protect mitochondrial energy production in endothelial cells
(ECs) from injury caused by hydrogen peroxide (H2O2)-induced oxidative damage in AS
through SIRT1/PGC-1α/ Nrf2 pathway.

Methods:
After preparation of containing serum, the cells were divided into various categories,
such as control group, H2O2 group (an oxidative damage model), HYQTD group, Selisistat
(EX527, a SIRT1 inhibitor) combined with H2O2 group, and EX527 combined with HYQTD
group. The evaluation of oxidative stress involved measuring reactive oxygen species (ROS) and
malondialdehyde (MDA) generation, as well as Superoxide Dismutase (SOD) activity. Mitochondrial
function and ultrastructure were measured by Transmission electron microscopy
(TEM), mitochondrial membrane potential (MMP), rate of oxygen consumption (OCR), respiratory
chain complex activities, and ATP production. The key proteins and gene levels in the
SIRT1/PGC-1α/Nrf2 pathway was quantified by quantitative real-time PCR (RT-PCR) and
Western blotting analysis.

Results:
We found oxidative stress, mitochondrial damage, and mitochondrial energy disorder in
H2O2-induced ECs. However it indicated a marked reversal after pretreated with HYQTD-DS.
Mechanistically, EX527 induced increased oxidative stress, worse mitochondrial dysfunction,
and less ATP synthesis.

Conclusion:
We demonstrated that HYQTD-DS attenuated oxidative stress, improved mitochondrial
function, and up-regulated mitochondrial ATP synthesis by activating SIRT1/PGC-
1α/Nrf2 pathway-induced mitochondrial biogenesis and its downstream NADH dehydrogenase
(ubiquinone) flavoprotein 2 (NDV2).
Effect of Combustion Material on the Level of Persistent Organic Pollutants in Smoked Pork
Authors: Uyen Nguyen Thu , Giang Do Hoang , Thuy Le Minh , Xuyen Nguyen Thi , Minh Nguyen Thi Thu , Duong Hoang Thuy , Le Bui Thi Nhat , Nhi Luu Hai , Anh Nguyen Thi Hong , Luyen Nguyen Thi , Minh Bui Quang , Nam Vu Duc , Dat Nguyen Tien 
Published: January 2025

Background:
Although smoking has historically been one of the most prevalent methods
for food preservation, it was considered a source of carcinogenic and mutagenic persistent organic
pollutants (POPs), such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls
(PCBs), and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) due to incompletely
combustion process. In this study, POPs in pork samples smoked with different materials: sawdust
only (NP group), sawdust and polyethylene (PE group), sawdust and polystyrene (PS group), and
mixed sawdust (M group) were detected and quantified.

Method:
The GC-MS method was applied to quantify several POPs in pork samples smoked by various
materials. After that, multivariate statistics were used to elucidate differences among groups and
chemical markers associated with each smoking material, underscoring the need to evaluate the implications
of smoking materials on food safety and public health.

Results:
The total PAH and PCB contents were lowest in the NP samples (smoked with sawdust
only), with PAH levels ranging from 500 to 2000 μg/kg lipid and PCB levels from 0.5 to 1.3 μg/kg
lipid. In contrast, the PE, PS, and M groups, which were smoked with sawdust combined with different
polymers and contaminants, showed higher levels. The PAH content in these groups exceeded
3000 μg/kg lipid, while PCB levels in PE and PS ranged from 1.5 to over 3.0 μg/kg lipid, and M
samples had the widest variation, spanning from 0.6 to 3.2 μg/kg lipid. Regarding PCDD/Fs, none
were detected in the NP samples, while the PE and PS groups contained between 1.2 and 1.5 pg/g
lipid, with specific compounds identified (e.g., 234678-HxCDF in PE and 12378-PeCDF and
123478-HxCDF in PS). The M samples exhibited the highest PCDD/Fs content, ranging from 5.0 to
14.0 pg/g lipid, with up to six compounds formed, including one PCDD and five PCDFs.

Conclusion:
The analysis also revealed that the presence of polyethylene and polystyrene significantly
elevated POP levels in the respective groups. Meanwhile, samples in the M group possessed
various chemical compositions with multiple concentrations, presented by the large area on the PCA
biplot and usually intermingled with samples of other groups. Especially, OCDD was the only PCDD
that was detected only in M group simples.
Semaphorin 3A Confers Protection against Oxidative Stress-induced Damage in Periodontal Ligament Stem Cells through the Activation of the
Wnt/β-catenin Signaling Pathway
Authors: He Haipeng , Yuan Xueshun , Chen Yanyan , Hu Tianyong , Cheng Baohui , Du Ruitian , Huang Jiamin , Geng Xiaorui , Li Hongwen , Liu Senqing , Liu Zhiqiang 
Published: January 2025

Objectives:
The osteogenic potential of periodontal ligament stem cells (PDLSCs) is
crucial for periodontal tissue regeneration. Prolonged and excessive oxidative stress (OS) impairs
the osteogenic function of PDLSCs. Recently, Semaphorin 3A (Sema3A) has been reported to
have multiple roles in bone protection. This study aimed to investigate the protective effect of Sema3A
on the osteogenic differentiation of PDLSCs under OS conditions induced by hydrogen peroxide
(H2O2).

Methods:
PDLSCs were subjected to H2O2 treatment to induce OS. The OS indices in PDLSCs
were evaluated by analyzing levels of reactive oxygen species (ROS), cell viability, and expression
of antioxidant factors using relevant assay kits. A small molecule inhibitor, XAV-939, was
employed to block the Wnt/β-catenin pathway. Osteogenic differentiation was assessed using alkaline
phosphatase (ALP) activity staining and Alizarin Red S (ARS) staining for mineralized nodules.
Expression levels of osteogenic gene markers and β-catenin were determined via real-time
quantitative polymerase chain reaction (RT-qPCR) or western blot (WB) analysis.

Results:
The stimulation of H2O2 induced OS in PDLSCs, resulting in a downregulation of Sema3A
expression and a decrease in osteogenic markers, including ALP activity, mineralized nodule
formation, and the expression of osteogenic genes (RUNX2 and ALP). However, the application
of recombinant human Sema3A (rhSema3A) counteracted H2O2-induced OS and restored these
osteogenic markers in PDLSCs under OS induced by H2O2. Mechanistic studies revealed that
these effects were associated with an upregulation of β-catenin levels. Moreover, inhibiting β--
catenin expression compromised the protective effect of Sema3A on osteogenesis in PDLSCs under
OS.

Conclusion:
Sema3A exerts a protective effect against H2O2-induced OS and activates the Wnt/β--
catenin pathway to restore osteogenic differentiation impaired by OS in PDLSCs.
Adenoviral Therapy for Cervical Cancer: From Targeted Modification to Immunotherapy
Authors: Li Yufeng , Zhang Haibin , Xin Wenhu , Qin Tiansheng 
Published: January 2025

Abstract:
Cervical cancer is a significant global health threat, ranking as the fourth most common malignancy
among women and resulting in over 300,000 deaths annually. Although screening and vaccination initiatives have
led to a decline in incidence rates, treatment options for advanced or recurrent cervical cancer remain inadequate,
often proving ineffective and costly. In this context, adenoviral therapy has emerged as a promising strategy to
enhance therapeutic outcomes. Adenoviruses are non-enveloped viruses that can efficiently infect a wide range of
cells, including tumor cells, while exhibiting a favorable safety profile, making them suitable candidates for clinical
applications. Adenoviral vectors possess the unique ability to package large segments of therapeutic genes,
allowing for diverse treatment approaches, including oncolytic virotherapy, which selectively targets and destroys
tumor cells while stimulating robust immune responses. By engineering adenoviruses to express tumor suppressor
genes such as p53, researchers can restore critical apoptotic pathways often disrupted in cervical cancer. Furthermore,
genetic modifications to capsid proteins can enhance the targeting of tumor cells and reduce the immunogenicity
associated with these viral vectors. Additionally, adenoviral vectors can serve as delivery systems for
therapeutic vaccines against HPV oncogenes E6 and E7, promoting effective immune responses and potentially
preventing disease progression. The combination of adenoviral therapy with immune checkpoint inhibitors offers
a novel approach to overcoming the immunosuppressive tumor microenvironment, enhancing overall antitumor
immunity. Overall, this review highlights the significant advancements in adenoviral therapy for cervical cancer,
emphasizing the need for further research to optimize these strategies and translate preclinical successes into
effective clinical applications. By harnessing the full potential of adenoviral vectors, we can improve treatment
options for patients who have cervical cancer, paving the way for more personalized and effective therapeutic
interventions.
Recent Advances in Bioactive Flavonoids-based Nanotherapeutics as Promising Neuroprotectants in Epilepsy
Authors: Pradhan Priyabrata , Jana Koushik , Ghosh Somnath , Debnath Biplab , Rout Saroj Kumar , Rai Vineet Kumar , Kar Biswakanth , Kar Durgamadhab , Ghosh Goutam , Rath Goutam 
Published: January 2025

Background:
Epilepsy is a prevalent neurological disorder that can be characterized
by seizures and can be caused by abnormal electrical impulses in the brain. Various genetic, environmental, age-related, and lifestyle factors are associated with its pathogenesis, which causes
neuronal cells to degenerate over time.

Methodology:
Epilepsy often results from an imbalance between excitatory neurotransmitters,
such as glutamate, and inhibitory neurotransmitters, such as GABA. Abnormalities in glutamate
receptors like N-methyl-D-aspartate (NMDA), and alpha-amino-3-hydroxy-5-methyl-4-
isoxazolepropionic acid (AMPA) can lead to excessive neuronal excitation, while dysfunctions
in GABA receptors can result in insufficient inhibition, both of which can provoke seizures. Additionally, a variety of receptors and pathways like NF-κB, DAPK, Trκb, COX-2, etc. are associated with the expression of epilepsy. This disorder often faces various limitations in treatment
with current anti-epileptic drugs (AEDs), such as drug resistance, adverse effects, and high costs.
In context, flavonoids exhibit significant neuroprotective properties in epilepsy through various
mechanisms such as antioxidant activity, anti-inflammatory effects, neurotransmitter systems,
and receptor modulation.

Results:
Flavonoids communicate with different signaling pathways and adjust their activities,
prompting valuable neuroprotective impacts. Essential flavonoids such as quercetin, rutin,
apigenin, luteolin, genistein, fisetin, chrysin, vitexin, naringin, baicalin, catechin, morin,
hesperetin, kaempferol, gallic acid, silibinin, wogonin, etc. have shown promising results in
channel regulation, reduced oxidative stress and neuroinflammation, and neuronal excitability in
experimental models of epilepsy. Given their inherent neuroprotective properties and ability to
modulate multiple pathways involved in epilepsy, flavonoids hold considerable promise as multitargeted, accessible, and low-cost alternatives to conventional AEDs. Although there are
challenges with target specificity and bioavailability, innovative approaches such as
nanotechnology and chemical modifications are being developed to enhance these aspects.

Conclusion:
Focusing on the mechanisms of action and neuroprotective benefits the paper
highlights the promising role of flavonoids and flavonoid-based nanotherapeutics as a beneficial
addition to epilepsy treatment strategies.
Research on Pole-climbing Robots that Can Perform Multi-degree-offreedom Motion and Working at the Top of Distribution Network Poles
Authors: Feng Yu , Wu Shaolei , Wu Kai , Guo Feng , Wei Chunjie , Wang Wei 
Published: January 2025

Background:
In order to complete power maintenance, equipment renewal and other
loss reduction work and reduce customer outage time at the same time, live electrical work has become
a necessary means. Pole-climbing robots, as an important auxiliary tool for power-carrying
operations, frequently appear in papers and patents.

Objective:
The pole climbing robot is designed to work at any position in the working space with
electric distribution pole, effectively and safely replacing manual work.

Methods:
Based on the physical parameters of the cement utility pole and the range of space for
live electrical work, the functional relationships are established between the structural parameters of
the robot and the kinematic and dynamical models, and the optimal range of parameters is solved.

Results:
The robot can rotate and climb around the pole with five steering angles: 0°, 22.5°, 45°,
67.5° and 90°. The control insulated operating rod can transport special tools to any station within
1m radius of the distribution pole for live work. It has a maximum load of 11kg and a maximum
climbing speed of 33.6mm/s.

Conclusion:
The pole-climbing robot can climb cement utility pole, manipulate the insulated operating
lever it carries, and transport special tools to the workstation on the cable for carrying out live
electrical work.
A Machine Learning Approach Using Topic Modeling to Identify and Assess Experiences of Patients With Colorectal Cancer: Explorative Study
Authors: Voigt Kelly , Sun Yingtao , Patandin Ayush , Hendriks Johanna , Goossens Richard Hendrik , Verhoef Cornelis , Husson Olga , Grünhagen Dirk , Jung Jiwon 
Published: January 2025

Abstract

Background
The rising number of cancer survivors and the shortage of health care professionals challenge the accessibility of cancer care. Health technologies are necessary for sustaining optimal patient journeys. To understand individuals’ daily lives during their patient journey, qualitative studies are crucial. However, not all patients wish to share their stories with researchers.

Objective
This study aims to identify and assess patient experiences on a large scale using a novel machine learning–supported approach, leveraging data from patient forums.

Methods
Forum posts of patients with colorectal cancer (CRC) from the Cancer Survivors Network USA were used as the data source. Topic modeling, as a part of machine learning, was used to recognize the topic patterns in the posts. Researchers read the most relevant 50 posts on each topic, dividing them into “home” or “hospital” contexts. A patient community journey map, derived from patients stories, was developed to visually illustrate our findings. CRC medical doctors and a quality-of-life expert evaluated the identified topics of patient experience and the map.

Results
Based on 212,107 posts, 37 topics and 10 upper clusters were produced. Dominant clusters included “Daily activities while living with CRC” (38,782, 18.3%) and “Understanding treatment including alternatives and adjuvant therapy” (31,577, 14.9%). Topics related to the home context had more emotional content compared with the hospital context. The patient community journey map was constructed based on these findings.

Conclusions
Our study highlighted the diverse concerns and experiences of patients with CRC. The more emotional content in home context discussions underscores the personal impact of CRC beyond clinical settings. Based on our study, we found that a machine learning-supported approach is a promising solution to analyze patients’ experiences. The innovative application of patient community journey mapping provides a unique perspective into the challenges in patients’ daily lives, which is essential for delivering appropriate support at the right moment.
Researchers and Research Data: Improving and Incentivising Sharing and Archiving
Authors: Ventsel Minna , Montague-Hellen Beth 
Published: January 2025

There has been a lot of discussion within the scientific community around the issues of reproducibility in research, with questions being raised about the integrity of research due to failure to reproduce or confirm the findings of some of the studies. Researchers need to adhere to the FAIR (findable, accessible, interoperable, and reusable) principles to contribute to collaborative and open science, but these open data principles can also support reproducibility and issues around ensuring data integrity. This article uses observations and metrics from data sharing and research integrity related activities, undertaken by a Research Integrity and Data Specialist at the Francis Crick Institute, to discuss potential reasons behind a slow uptake of FAIR data practices. We then suggest solutions undertaken at the Francis Crick institute which can be followed by institutes and universities to improve the integrity of research from a data perspective. One major solution discussed is the implementation of a data archive system at the Francis Crick Institute to ensure the integrity of data long term, comply with our funders’ data management requirements, and to safeguard our researchers against any potential research integrity allegations in the future.
Factores emergentes para la comprensión de calor y temperatura en jóvenes de educación media en un colegio público en Bogotá Colombia
Authors: Jaramillo-Pinto Luisa María , Jardey Suárez Oscar , Gamboa-Suárez Audin Aloiso 
Published: January 2025

El estudio de las nociones de calor y temperatura en la enseñanza de la termodinámica, resultan siendo objeto de estudio estructural. Para el caso de la investigación, el objetivo es identificar los factores intrínsecos en la comprensión de calor y temperatura, en dos grupos de jóvenes estudiantes de educación media, en un colegio público de la ciudad de Bogotá-Colombia, al utilizar videos tutoriales y trabajo entre pares, como mediación en su estudio. Teóricamente, se fundamenta en las pedagogías activas, así como en las nociones de calor y temperatura aceptadas en el contexto de la física. El enfoque epistemológico predominante es cuantitativo, con dos grupos y tres observaciones (pretest, pos-test y pos-pos-test). El instrumento utilizado es el de Heat and Temperature Conceptual Evaluation (HTCE). Los resultados muestran que los factores a priori (Calor y temperatura, Tasa de enfriamiento, Calorimetría, Tasa de transferencia de calor, Percepción de temperatura, Cambio de fase y Conductividad térmica) hay un mayor avance en la comprensión de las nociones de calor y temperatura, del estudiantado del grupo con pedagogías activas; sin embargo, después de la interacción entre pares, los grupos incrementan sustancial y estadísticamente significativas, las puntuaciones medias. A manera de conclusión, las pedagogías activas, en particular el trabajo entre pares, articulados con la actividad experimental y los video tutoriales, son elementos que contribuyen a la comprensión de las nociones de calor y temperatura.
Proyecto ambiental escolar- PRAE como contribución a la cultura socio-ambiental del Megacolegio La Frontera del Municipio de Villa del Rosario Norte de Santander
Authors: Mendoza-Ríos Johanatan Darío 
Published: January 2025

Introducción: La presente investigación busca contribuir en la construcción de la cultura socio-ambiental desde la comunicación social en perspectiva del- PRAE del Megacolegio La Frontera de Villa del Rosario a través de una estrategia de multimedia que permita dinamizar su formación integral. Pregunta de investigación: ¿Hay Articulación del PRAE con el PEI de la Institución educativa o está aislados? ¿Cuáles son las potencialidades o fortalezas del PRAE, en relación con la problemática de la localidad?Objetivo: i) Contribuir en la construcción de la cultura socio-ambiental desde la comunicación social en perspectiva del Proyecto Ambiental Escolar- PRAE del Megacolegio La Frontera de Villa del Rosario a través de una estrategia multimedia que permita dinamizar su formación integral. Metodología: Para esta investigación se realizó un enfoque cualitativo puesto que el objetivo es contribuir en la construcción de la cultura socio-ambiental desde la comunicación social en perspectiva del PRAE del Megacolegio La Frontera de Villa del Rosario a través de una estrategia multimedia que permita dinamizar su formación integral Resultados: Los resultados metodológicos exponen la realización de los objetivos a una población afectada, desde los ámbitos socio – cultural de tal forma que permitió determinar las causas, y el alcance sobre la zona de estudio. Conclusiones: Se identifica las potencialidades y debilidades del PRAE, las debilidades y potencialidades ambientales dejan un impacto positivo y negativo de los mismos no sólo en los sistemas naturales, sino también en los sistemas sociales.
Application of Neural Networks for Solving Nonlinear Boundary Problems for Complex-Shaped Domains
Authors: Galaburdin A. V. 
Published: January 2025

Introduction. Many practically significant tasks reduce to nonlinear differential equations. In this study, one of the applications of neural networks for solving specific nonlinear boundary problems for complex-shaped domains is considered. Specifically, the focus is on solving a stationary heat conduction differential equation with a thermal conductivity coefficient dependent on temperature.Materials and Methods. The original nonlinear boundary problem is linearized through Kirchhoff transformation. A neural network is constructed to solve the resulting linear boundary problem. In this context, derivatives of singular solutions to the Laplace equation are used as activation functions, and these singular points are distributed along closed curves encompassing the boundary of the domain. The weights of the network were tuned by minimizing the mean squared error of training. Results. Results for the heat conduction problem are obtained for various complex-shaped domains and different forms of dependence of the thermal conductivity coefficient on temperature. The results are presented in tables that contain the exact solution and the solution obtained using the neural network. Discussion and Conclusion. Based on the computational results, it can be concluded that the proposed method is sufficiently effective for solving the specified type of boundary problems. The use of derivatives of singular solutions to the Laplace equation as activation functions appears to be a promising approach.