bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utilizing DNA barcoding approach to study the diversity of the blue swimming crab from Tuban District, East Java, Indonesia
Authors: JOESIDAWATI MARITA IKA , NURSALIM NINING , KHOLILAH NENIK , KURNIASIH EKA MAYA , CAHYANI NI KADEK DITA , AMBARIYANTO AMBARIYANTO 
Published: January 2024

Abstract. Joesidawati MI, Nursalim N, Kholilah N, Kurniasih EM, Cahyani NKD, Ambariyanto A. 2023. Utilizing DNA barcoding approach to study the diversity of the blue swimming crab from Tuban District, East Java, Indonesia. Biodiversitas 24: 4731-4737. Blue swimming crabs are one of the economically significant fisheries resources in Indonesia. However, Indonesia's blue swimming crab fisheries still depend on wild catches that lead to a non-target species harvest, especially the cryptic swimming crabs. This study aims to investigate the genetic diversity of blue swimming crab specimens and other cryptic species using a molecular approach (DNA Barcoding). The samples were caught from the waters of Tuban District, East Java, Indonesia, using traditional foldable traps or "bubu lipat". This study utilized the Polymerase Chain Reaction (PCR) method targeting the Cytochrome c Oxidase Subunit I (COI) gene. The sequences were then compared to the NCBI database to get the closest species based on the sequences. 21 out of 23 samples were identified as Portunus pelagicus, and two samples were identified as Charybdis feriata with percentage of similarity ranging from 99.68% to 100%. The phylogenetic trees were constructed using Neighbor Joining and Maximum Likelihood method with sequences from Scylla serrataas an outgroup. The genetic distance between P. pelagicus and C. feriata was 16%, whereas the genetic distance between P. pelagicus and C. feriata to the outgroup was 20%. The molecular method is crucial for species identification and population stock assessment. This information helps in determining stock boundaries, estimating population sizes, and understanding the genetic health of populations. Genetic data will help the government to have a better management of the fisheries activities in Indonesia by providing the diversity and population data.
Isolation of bacteria displaying potent antagonistic activity against fungi causes anthracnose disease in chili
Authors: TRAM TRAN THI NGOC , QUANG HOANG TAN , VU NGUYEN QUANG HOANG , NGUYEN PHAN THI THAO , THI TON NU MINH , PHUONG TRUONG THI BICH , THI PHAM THI DIEM 
Published: January 2024

Abstract. Tram TTN, Quang HT, Vu NQH, Nguyen PTT, Thi TNM, Phuong TTB, Thi PTD. 2023. Isolation of bacteria displaying potent antagonistic activity against fungi causes anthracnose disease in chili. Biodiversitas 24: 4919-4926. Anthracnose is a common disease on many crops caused by Colletotrichum species, and it leaves serious consequences, especially on chili. Beta-1,3 glucanase is a semi-constitutive hydrolytic enzyme that is involved in the biological regulation of plant pathogens. Its main function is the breakdown of ?-1,3-glucan, a major constituent of the fungal cell wall. In a study conducted in Vietnam's Quang Tri and Thua Thien Hue Provinces, researchers isolated five bacterial strains from soil where chili plants were grown. To induce the production of beta-1,3 glucanase, these isolates were cultivated in a nutrient-rich medium supplemented with 1% laminarin. The activity of beta-1,3-glucanase in the bacterial strains ranged from 0.31 to 1.72 U mL-1, and antagonistic activity against Colletotrichum spp. causes of anthracnose reached from 50.68 to 69.04%. Strain AT4 had strong antagonistic activity against C. scovillei HUCL1, C. siamensis PV6, and CL3 of 76.44, 62.65, and 68.04%, respectively. Molecular identification based on 16S-rRNA sequences showed that the strains were Paenibacillus polymyxa AT4, B. siamensis ML3, B. tequilensis ML4 and ML6, and B. velezensis GL7. AT4 and ML6 are potential strains for the control of anthracnose diseases caused by Colletotrichum spp. on chili peppers.
Response diversity and traits related to pepper yellow leaf curl disease resilience for resistant plants selection
Authors: SAYEKTI TRI WAHONO DYAH AYU , SYUKUR MUHAMAD , HIDAYAT SRI HENDRASTUTI , MAHARIJAYA AWANG , SOBIR SOBIR 
Published: January 2024

Abstract. Sayekti TWDA, Syukur M, Hidayat SH, Maharijaya A, Sobir. 2023. Response diversity and traits related to pepper yellow leaf curl disease resilience for resistant plants selection. Biodiversitas 24: 5057-5064. Many chili species (Capsicum spp.) have been used and consumed, among them five species, namely Capsicum annuum, C. frutescens, C. chinense, C. baccatum and C. pubescens, are widely used. Most of these varieties are susceptible to Pepper Yellow Leaf Curl Disease (PYLCD) caused by Pepper yellow leaf curl virus (PYLCV) (Begomovirus, Geminiviridae). To control PYLCD, it is essential to gather resistant plant varieties to effectively shield cultivated plants from viral infections. An effective selection activity related to the target character is needed to get highly productive resistant plants. The objective of this study was to evaluate the relationship between resistance characters to crop production and to obtain appropriate selection criteria to increase the effectiveness of selecting resistant plants with good production. This study was conducted at Cikabayan Field, IPB University, Bogor, Indonesia. Twenty-nine genotypes of chili pepper were used, which consists of four species, including C. annuum, C. frutescens, C. chinense, and C. baccatum, arranged in a nested block design with three replications. The observed symptoms were yellowing of leaves followed by leaf malformations, but there were differences in the yellowing symptoms. From observation on visual symptoms, resistance characteristics, plant performance and productivity, there were differences in the response of each species to PYLCV attack. Correlation and path analysis results show that there was also differences in performance drop between C. annuum species and the non-C. annuum group. In C. frutescens, C. chinense, and C. baccatum, direct selection on resistance characters was able to increase the resistance level of selected plants while maintaining good crop production. Whereas for C. annuum species, the plant resistance level was not correlate with productivity reduction, so in the selection process, the percentage of yield decline needs to be considered to obtain resistant plants and maintain crop production.
Forests over limestone in Guiuan, Eastern Samar, the Philippines: Species composition and influencing environmental factors
Authors: BUOT JR. INOCENCIO E. , VILLANUEVA ELAINE LOREEN C. , ORIGENES MARNE G. , OBEÑA REN DIVIEN R. 
Published: January 2024

Abstract. Buot Jr IE, Villanueva ELC, Origenes MG, Obeña RDR. 2023. Forests over limestone in Guiuan, Eastern Samar, the Philippines: Species composition and influencing environmental factors. Biodiversitas 24: 5123-5136. Forests over limestone are critical ecosystems providing numerous ecosystem services. However, the ecology of these ecosystems is less studied like the Guiuan Marine Resource Protected Landscape and Seascape (GMRPLS) in Eastern Samar, Philippines which incidentally is affected by strong typhoons. Studies on forests over limestone are even more essential to understand the vegetation ecology providing insights in crafting appropriate management strategy to sustain ecosystem services. Hence, the study aims to identify the woody vegetation diversity and composition of forests over limestone in GMRPLS, and to analyze the environmental factors influencing the physiognomy and ecology of the forests. Standard vegetation techniques were used and a total of nine plots with size of 20 × 20m each were established to assess the tree species (?1m height). Plant abundance data were recorded and computed. Soil samples were collected while climate data were obtained from the Guiuan, Eastern Samar Weather Station. Cluster analysis using relative density of trees per plot was done in order to identify major plant communities. Subsequently, a canonical correspondence analysis was carried out to pinpoint which environmental variables influenced the plant data set. Results show a total of 37 species belonging to 17 families, with 18 species are listed under by IUCN and seven species are listed by DAO 2017-11. Cluster analysis indicated 3 vegetation groupings or clusters named after the dominant species per plot, namely Cluster I (Hancea-Calophyllum-Macaranga-Ficus-Gnetum), Cluster II (Monoon-Wallaceodendron-Artocarpus-Hancea-Bago-eho), and Cluster III (Bridelia-Monoon-Neonauclea-Gnetum-Artocarpus-Sterculia-Hancea). GUI 02 plot (Cluster 1) was observed as the most diverse with the highest Simpson’s index and Fisher’s alpha, as well as the highest in total number of tree species. Canonical correspondence analysis indicated elevation, temperature and anthropogenic disturbances as the major factors influencing the vegetation diversity and composition. This can be used to formulate forest management strategies for the sustainable conservation of forests over limestone in GMRPLS.
Genetic variation and phylogenetic position of Taxus sumatrana (Miq.) de Laub. (Taxaceae) in Sumatra, Indonesia based on trnL-trnF region
Authors: KAMAL IRSYAD , ARROFAHA NAWWALL , SUSILA SUSILA , YULITA KUSUMADEWI SRI , RACHMAT HENTI HENDALASTUTI , SUSILOWATI ARIDA 
Published: January 2024

Abstract. Kamal I, Arrofaha N, Susila, Yulita KS, Rachmat HH, Susilowati A. 2023. Genetic variation and phylogenetic position of Taxus sumatrana (Miq.) de Laub. (Taxaceae) in Sumatra, Indonesia based on trnL-trnF region. Biodiversitas 24: 5155-5166. Taxus sumatrana (Miq.) de Laub. is a synonym of T. wallichiana Zucc. by some authors based on morphological characters and its distribution. This species is a medicinal plant and contains taxol as an anti-cancer with slow natural regeneration rate and increasing threats to their existence. This study aimed to determine the genetic diversity and the phylogenetic analysis using trnL-trnF for T. sumatrana growing in Sumatra, Indonesia. The total of 37 individuals from three natural populations of T. sumatrana were studied, consisting of the current new collection from Toba - North Sumatra and previously studied population of Dempo - South Sumatra and Sungai Penuh, Kerinci Seblat National Park - Jambi. The Toba population did not show variation at individual level within the population as similar results were obtained from the previous study. However, one indel (- and A) was found at 217 bp from all three T. sumatrana populations from Sumatra, Indonesia. When the sequences were aligned with the reference sequence from GenBank, single base substitutions were found at 344, 478,722, and 757 bp consisting of one indel and three single nucleotide polymorphisms (SNPs). Phylogeny tree showed that T. sumatrana from Sumatra population clustered together with T. mairei and T. wallichiana var. mairei and later combined with other T. sumatrana available in NCBI.
Carrying capacity estimation and habitat suitability of Sumatran elephant in Datuk Gedang Wildlife Corridor, Bukit Tigapuluh Landscape, Jambi, Indonesia
Authors: FIKRI NAUFAL , RAHMAN DEDE AULIA , SANTOSO NYOTO 
Published: January 2024

Abstract. Fikri N, Rahman DA, Santoso N. 2023. Carrying capacity estimation and habitat suitability of Sumatran elephant in Datuk Gedang Wildlife Corridor, Bukit Tigapuluh Landscape, Jambi, Indonesia. Biodiversitas 24: 5548-5557. Rapid land use changes, including deforestation and land conversion into human areas, lead to the fragmentation of Sumatran elephant (Elephas maximus sumatranus) habitat. This study aimed to assess food capacity and determine habitat suitability for Sumatra elephants in Datuk Gedang Wildlife Corridor Bukit Tigapuluh Landscape. The research was conducted from January to May 2023. Data on potential, productivity, and carrying food capacity were collected with a direct method in the field with vegetation analysis. Carrying capacity estimation is based on data on productivity, land cover area, proper use, and Sumatran elephant food needs in a day. The habitat suitability model used the present coordinate point data from the GPS collar period of January-December 2022 with eight environment variables. The data were then analyzed for the build Sumatera Elephant habitat suitability model using the Maximum Entropy (MaxEnt) algorithm. We recorded 95 species of the potential Sumatran elephant food. The total feed productivity is 1,032,740.722 kg/ha /day and the carrying capacity is 258 elephants. There were three models of habitat suitability categories: high 4,238.66 ha (7%), medium 8,522.34 ha (14%), and low 11,130.36 ha (18%). The studied landscape can adequately accommodate the current population of Sumatran elephants depending on landscape governance (policy and spatial planning) outside conservation areas.
Diversity of root bacterial community associated with seven orchid species from Mount Merbabu National Park, Central Java, Indonesia
Authors: PANGASTUTI ARTINI , PITOYO ARI , SUSILOWATI ARI , MEISARI RIZKA , YULIANA IRVY , AULIA KHATSANATUL , PUSPITASARI LIANA PUTRI , RUSIANI RUSIANI , PRASOJO HENDRO 
Published: January 2024

Abstract. Pangastuti A, Pitoyo A, Susilowati A, Meisari R, Yuliana I, Aulia K, Puspitasari LP, Rusiani, Prasojo H. 2023. Diversity of root bacterial community associated with seven orchid species from Mount Merbabu National Park, Central Java, Indonesia. Biodiversitas 24: 5676-5684. Indonesia has a high diversity of wild orchids. One of the orchid habitats that has not been widely explored is Mount Merbabu National Park (TNGMb), Central Java, Indonesia. The dependence of orchids on specific microorganisms for survival and growth, especially during early development, has not been widely studied. Endophytes, such as bacteria, can help the host plant grow, tolerate stress, resist disease, acquire nutrients, or form symbiotic relationships. However, bacterial endophytes associated with orchids have not been extensively explored. This research examined the diversity of bacteria associated with orchid roots in Mount Merbabu National Park using a culture-independent method. Seven orchid species were sampled for root bacterial community analysis, that identified as Spathoglottis plicata, Dendrobium sagittatum, Malaxis kobi, Apendicula alba, Pholidota carnea, Dendrobium tenellum, and Bulbophyllum compressa. Bacterial diversity in all orchid roots was high, with several dominant bacterial species. Proteobacteria, Firmicutes, Bacteriodota, and Actinobacteriota were the four most abundant phyla, with Proteobacteria having the highest abundance (37 to 90%) in all samples. The top ten bacteria genera were Pseudomonas, Serratia, Rhodanobacter, Acinetobacter, Escherichia, Bifidobacterium, Clostridium, Parabulkholderia, Faecalibacterium, and Muribaculaceae family that could not be identified at the genus level. Overall, 61, 42, 838, 98, 78, 973, and 1383 OTUs were unique to S. plicata, D. sagittatum, M. kobi, A. alba, P. carnea, D. tenellum, and B. compressa, respectively, whereas all seven species shared 335 OTUs. Our research suggested that environmental factors and the host plant's genetics affect the plant microbiome's composition and diversity. These elements might be essential for ecosystem function and conservation.
Morpho-physiology and metabolite content of Cosmos caudatus Kunth. and yellow and orange Cosmos sulphureus Cav.
Authors: SALEH ISMAIL , AZIZ SANDRA ARIFIN , MELATI MAYA , ANDARWULAN NURI 
Published: January 2024

Abstract. Saleh I, Aziz SA, Melati M, Andarwulan N. 2023. Morpho-physiology and metabolite content of yellow and orange Cosmos sulphureus Cav. and Cosmos caudatus Kunth. Biodiversitas 24: 5739-5746. Cosmos sp., known as kenikir in Indonesia, is an underutilized vegetable commodity. Cosmos sulphureus Cav. (C. sulphureus), with orange and yellow flowers, is commonly found as an ornamental plant. C. sulphureus can also be used as vegetable crops, although the Cosmos species widely used as a vegetable is Cosmos caudatus Kunth. (C. caudatus). This study investigated the growth, shoot production, primary and secondary metabolite content, and antioxidant activity of yellow and orange C. sulphureus and C. caudatus. This experiment was arranged using a Randomized Completely Block Design (RCBD) with one factor: Cosmos species (yellow and orange C. sulphureus and C. caudatus) with six replications. The result showed that plant height, leaf number, and branch number were not significantly different among the three Cosmos studied; however, the shoot weight per plant of orange C. sulphureus was significantly higher than the others. The antioxidant activity and phenol content of C. sulphureus were lower than that of C. caudatus. Meanwhile, crude fiber, flavonoid, anthocyanin, and carotenoid content were not significantly different among the three kinds of Cosmos. Orange C. sulphureus could be a functional vegetable, although its antioxidant activity is lower than C. caudatus.
Effects of meliponiculture Tetragonula laeviceps on pollinator diversity and visitation rate and citrus productivity in West Java, Indonesia
Authors: NURDIANSYAH MUHAMAD ALDI , Abduh Muhammad Yusuf , Permana Agus Dana 
Published: January 2024

Abstract. Nurdiansyah MA, Abduh MY, Permana AD. 2023. Effects of meliponiculture Tetragonula laeviceps on pollinator diversity and visitation rate and citrus productivity in West Java, Indonesia. Biodiversitas 24: 5757-5763. Citrus reticulata var. Rimau Gerga Lebong (RGL) is a prominent Indonesian citrus variety, yet its global productivity is facing challenges due to decreasing diversity in pollinators. This study aimed to bolster the productivity of C. reticulata var. RGL in Bandung by introducing Tetragonula laeviceps Smith 1857 (Hymenoptera: Apidae). Simultaneously, the study explored the influence of pollinator diversity, foraging behavior, and visitation rates and compared the results with open and wind pollination. Field investigations were conducted to observe the pollination activities of T. laeviceps and eight wild pollinator species, including Apis cerana Fabricius 1793, Ceratina cognata Smith 1879, Xylocopa confusa Pérez 1901, Xylocopa latipes Drury 1773, Vespa affinis Linnaeus 1764, Dolichoderus thoracicus Smith 1860, Papilio demoleus Linnaeus 1758, and Catopsilia pyrantel Linnaeus 1758. T. laeviceps exhibited the highest relative abundance (34.87%) and an average time spent of 72.11 seconds per flower. Visitation rates were highest during noon (10:00 to 13:00) at 0.31 pollinators/hour/flower, influenced by temperature, relative humidity, and light intensity. The phenological stages of C. reticulata var. RGL spanned 240 days. Fruit set, fruit weight, and estimated citrus productivity were significantly higher in T. laeviceps and open pollination compared to wind pollination. This study highlights the suitability of T. laeviceps as an effective pollinator for C. reticulata var. RGL. The findings have implications for the conservation and management of pollinator populations to ensure sustainable citrus production in the declining global diversity.
The potential of bamboo and the contribution of bamboo craftsmen to the community's income in Cibadak Village, Bogor City, Indonesia
Authors: Muttaqin Zainal , Rusli Abdul Rahman , Supriyanto Bambang 
Published: January 2024

Abstract. Muttaqin Z, Rusli AR, Supriyanto B. 2023. The potential of bamboo and the contribution of bamboo craftsmen to the community's income in Cibadak Village, Bogor City, Indonesia. Biodiversitas 24: 5796-5807. Bamboo is a plant of rural communities that can be used for various purposes in people's lives. Cibadak Village is thought to have a substantial number of bamboo potentials, but the exact amount has yet to be discovered. Likewise, the contribution of income obtained from bamboo-cage craftsmen is also unknown, so studies on this issue are essential. This research aimed to determine the potential availability of plants and the contribution of bamboo craftsmen to the income of the people of Cibadak Village, Tanah Sareal Sub-district, Bogor City, West Java Province, Indonesia. The collection of data was conducted through field research and bibliographic research. This research concluded that the potential of bamboo in Cibadak Village was only 6.72 ha or 2.04% of the total area, and the contribution of bamboo craftsmen to the community's income was less significant (27.03%) compared to the average total income of the community. However, the income of bamboo craftsmen more than the other income contribution came from drivers (25.00%), merchants (21.67%), and construction workers (26,30%). Only 20% of respondents consider the revenue of bamboo craftsmen as the main job, and 80% of the revenue is side income to complete revenue from the other job. The species of bamboo were found of bambu tali (Gigantochloa apus), bambu betung (Dendrocalamus asper), bambu ampel hijau (Bambusa vulgaris var. vitata), bambu ampel kuning (Bambusa vulgaris var. striata), and bambu andong (Gigantochloa pseudoarundinacea). Bambu tali are the most commonly found in Cibadak Village, consisting of 279 clumps (80.87%) and 25,028 culms (90.70%), while other bamboo species were found in small numbers. The bamboo craftsmen use all raw materials of Bambu tali (Gigantochloa apus), and a few people also utilize bamboo shoots of Bambu betung and Bambu ampel for self-consumption as complementary food vegetables.
Liberalism and Shia Political Thought in the Thought of Allameh Naieni, A Theoretical Framework for the Iranian Constitutional Revolution
Authors: Barkhordari Aref 
Published: January 2024

The purpose of writing this article is to analyze and describe the political thought of Allameh Mohammad Hossein Naeini as one of the most important theorists of the Iranian Constitutional Revolution (1284 AH), who tried to reconcile the components of liberalism and classical Shiite political thought to provide a Theoretical basis for the Iranian Constitutional Revolution. In this article, the treatise "Tanbih al-Ulama and Tanzih al-Mulla is, the most important work of Naeini and some other works that have studied her thought, are examined. In Iranian Constitutional Revolution, those who defended the Constitution against supporters of tyranny began to write their treatises, mainly during period of minor tyrann[1]. The most important goal of these thinkers in writing the treatises is to eliminate tyranny, reduce king, s power and rulers in political system of Qajar period and limiting them to the law of parliament and public will on one hand and explaining the concepts and foundations of liberalism and constitutionalism in a way that is compatible with Islamic law on the other hand. In justifying constitutionalism these treatises has referred more to weaknesses and harms of authoritarian system, as well as to poverty and backwardness of Iranian society, and considered constitutionalism to be the only solution to problems.The aim of these dissertations was to demonstrate the efficiencies and capabilities of Constitution in preserving and strengthening the Iranian society. In fact, they are in line with times have sought to implement a religious copy of liberal system and Western constitution in Iranian society. The most important treatise that written in this field is the book of Tanbih al-Allameh and Tanzih al-Mella written by Mohammad Hussein Naeini which we will examine his theories in this article.