bluuebunny/crossref_metadata_2025_split huggingface dataset:
ResCol‐Versuche zur Prüfung der dynamischen Langzeitstabilität von TA/TM‐Böden unter Eisenbahnverkehr
Authors: Hu Yifeng , Haupt Wolfgang , Müllner Bernd 
Published: July 2004

AbstractZur Bewertung der dynamischen Langzeitstabilität wurden ResCol‐Versuche an Tertiärtonen (TA/TM Böden), die aus den erdstatisch abzusichernden Zonen im Einschnittsbereich des Loses Süd der Neubaustrecke Nürnberg–Ingolstadt entnommen wurden, durchgeführt. Aus den Versuchsergebnissen wurden die Eingangsparameter für die dynamische Bemessung mit einem Verfahren, das aufgrund bekannter bodendynamischer Erkenntnisse speziell für die Erdbauwerke unter Eisenbahnverkehr entwickelt worden ist, ermittelt. Damit wurden für die untersuchten Böden die Prüfkriterien bei der Bauausführung hinsichtlich dynamischer Langzeitstabilität abgeleitet. In diesem Beitrag werden das Bemessungsverfahren, die Versuchsergebnisse und ihre Anwendung in der Praxis beschrieben.
Mechanisms of biosorption of different heavy metals by brown marine macroalgae
Authors: Raize Ofer , Argaman Yerachmiel , Yannai Shmuel 
Published: July 2004

AbstractThe biosorption mechanisms of different heavy metallic cations (Cd, Ni, Pb) to active chemical groups on the cell wall matrix of the nonliving brown marine macroalga, Sargassum vulgaris in its natural form, were examined by the following instrumental and chemical techniques: Fourier‐transform infrared (FTIR) analysis, X‐ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and extraction of alginic acid and sulfated polysaccharides, which act as metal‐binding moieties present in cell wall. From the different techniques used and the known chemical composition of the algal cell wall, it was observed that biosorption of the metallic cations to the algal cell wall component was a surface process. The binding capacities of the different metal cations were between 1 and 1.2 mmol metal/g on a dry weight basis. The main chemical groups involved in the metallic cation biosorption were apparently carboxyl, amino, sulfhydryl, and sulfonate. These groups were part of the algal cell wall structural polymers, namely, polysaccharides (alginic acid, sulfated polysaccharides), proteins, and peptidoglycans. The main cadmium cation sequestration mechanism by the algal biomass was apparently chelation, while the nickel cation sequestration mechanism was mainly ion exchange. Lead cations exhibit higher affinity to the algal biomass, and their binding mechanism included a combination of ion exchange, chelation, and reduction reactions, accompanied by metallic lead precipitation on the cell wall matrix. During the ion exchange process, calcium, magnesium, hydrogen cations, and probably other cations (sodium and potassium) in the algal cell wall matrix were replaced by the tested heavy metals. © 2004 Wiley Periodicals, Inc.
Growth‐rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose‐phosphate pathway
Authors: Flores Salvador , de Anda‐Herrera Ramón , Gosset Guillermo , Bolívar Francisco G. 
Published: July 2004

AbstractExpression of plasmid‐encoded genes in bacteria is the most common strategy for the production of specific proteins in biotechnological processes. However, the synthesis of plasmid‐encoded proteins and plasmid‐DNA replication often places a metabolic load (metabolic burden) into the cell's biochemical capacities that usually reduces the growth rate of the producing culture (Glick BR. Biotechnol Adv 1995;13:247–261). This metabolic burden may be related to a limited capacity of the cell to supply the extra demand of building blocks and energy required to replicate plasmid DNA and express foreign multicopy genes. Some of these required blocks are intermediaries of the pentose phosphate (PP) pathway, e.g., ribose‐5‐phosphate, erythrose‐4‐phosphate. Due to the important impact of metabolic burden on biotechnological processes, several groups have worked on developing strategies to overcome this problem, like reduction of plasmid copy number (Seo JH, Bailey JE. Biotechnol Bioeng 1985;27:1668–1674; Jones KL, Kim S, Keasling JD. Metab Eng 2000;3:328–338), chromosomal insertion of the gene which product is desired, or changing the plasmid‐coded antibiotic resistance gene (Hong Y, Pasternak JJ, Glick BR. Can J Microbiol 1995;41:624–628). However, few efforts have been attempted to overcome the reduction of growth rate due to protein over‐expression, by modifying central metabolic pathways (Chou C‐H, Bennett GN, San KY. Biotechnol Bioeng 1994;44:952–960). We constructed a high‐copy number plasmid carrying the gene for glucose‐6‐phosphate dehydrogenase, zwf, under the control of an inducible trc promoter (pTRzwf04 plasmid). By transforming a wild‐type strain and inducing with IPTG, it was possible to recover growth‐rate from 0.46 h−1 (uninduced) to 0.64 h−1 (induced). The same transformation in an Escherichia coli zwf−, allows a growth‐rate recovery from 0.43 h−1 (uninduced) to 0.62 h−1 (induced). We also studied this effect as part of a laboratory‐scale biotechnology process: production of a recombinant insulin peptide by co‐transforming E. coli JM101 strain with pTRzwf07, a low‐copy‐number plasmid that carries the same inducible construction as pTRzwf04, and with the pTEXP‐MMRPI vector that carries a TrpLE‐proinsulin hybrid gene. In this system, production of TrpLE‐proinsulin strongly reduces growth rate; however, overexpression of zwf gene recovers with a growth rate from 0.1 h−1 in the TrpLE‐proinsulin induced strain, to 0.37 h−1 when both zwf and TrpLE‐proinsulin genes were induced. In this paper, we show that the engineering of the pentose phosphate pathway by modulation of the zwf gene expression level partially overcomes the possible bottleneck for the supply of building blocks and reducing power synthesized through the PP pathway, that are required for plasmid replication and plasmid‐encoded protein expression. © 2004 Wiley Periodicals, Inc.
Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm
Authors: Jewett Michael C. , Swartz James R. 
Published: July 2004

AbstractCytoplasmic mimicry has recently led to the development of a novel method for cell‐free protein synthesis called the “Cytomim” system. In vitro translation with this new system produced more than a 5‐fold yield increase of chloramphenicol acetyl transferase (CAT) relative to a conventional method using pyruvate as an energy substrate. Factors responsible for activating enhanced protein yields, and causes leading to protein synthesis termination have been assessed in this new system. Enhanced yields were caused by the combination of three changes: growing the extract source cells on 2× YTPG media versus 2× YT, replacing polyethylene glycol with spermidine and putrescine, and reducing the magnesium concentration from conventional levels. Cessation of protein synthesis was primarily caused by depletion of cysteine, serine, CTP, and UTP. Substrate replenishment of consumed amino acids, CTP, and UTP extended the duration of protein synthesis to 24 h in fed‐batch operation and produced 1.2 mg/mL of CAT. By also adding more T7 RNA polymerase and plasmid DNA, yields were further improved to 1.4 mg/mL of CAT. These results underscore the critical role that nucleotides play in the combined transcription–translation reaction and highlight the importance of understanding metabolic processes influencing substrate depletion. © 2004 Wiley Periodicals, Inc.
BioLogic gates enable logical transcription control in mammalian cells
Authors: Kramer Beat P. , Fischer Cornelius , Fussenegger Martin 
Published: July 2004

AbstractThe architecture of gene regulatory networks is reminiscent of electronic circuits. Modular building blocks that respond in a logical way to one or several inputs are connected to perform a variety of complex tasks. Gene circuit engineers have pioneered the construction of artificial gene regulatory networks with the intention to pave the way for the construction of therapeutic gene circuits for next‐generation gene therapy approaches. However, due to the lack of a critical amount of eukaryotic cell‐compatible gene regulation systems, the field has so far been limited to prokaryotes. Recent development of several mammalian cell‐compatible expression control systems laid the foundations for the assembly of transcription control modules that can respond to several inputs. Herein, three approaches to evoke combinatorial transcription control have been followed: (i) construction of artificial promoters with up to three operator sites for regulatory proteins, and (ii) parallel and (iii) serial linking of two gene regulation systems. We have combined tetracycline‐, streptogramin‐, macrolide‐, and butyrolactone transcription control systems to engineer BioLogic gates of the NOT IF‐, AND‐, NOT IF IF‐, NAND‐, OR‐, NOR‐, and INVERTER‐type in mammalian cells, which are able to respond to up to three different small molecule inputs. BioLogic gates enable logical transcriptional control in mammalian cells and, in combination with modern transduction technologies, could serve as versatile tools for regulated gene expression and as building blocks for complex artificial gene regulatory networks for applications in gene therapy, tissue engineering, and biotechnology. © 2004 Wiley Periodicals, Inc.
Dinuclear Barium(II) Complexes Based on a Calix[4]arene Scaffold as Catalysts of Acyl Transfer
Authors: Cacciapaglia Roberta , Casnati Alessandro , Di Stefano Stefano , Mandolini Luigi , Paolemili Daniele , Reinhoudt David N. , Sartori Andrea , Ungaro Rocco 
Published: July 2004

AbstractTwo novel regioisomeric calix[4]arene derivatives (2 and 3), decorated with two aza[18]crown‐6 units at vicinal (1,2) or diagonal (1,3) positions of the upper rim, were synthesized. The catalytic activities of their dinuclear Ba2+ complexes were investigated in the ethanolysis of esters 8–11, endowed with a carboxylate anchoring group. Major results are as follows: 1) the two metal ions in the dinuclear catalysts work together in a cooperative fashion; 2) the vicinal calix[4]arene catalyst 2 is far superior to its diagonal regioisomer 3 in the reactions of all of the investigated esters; and 3) the distance between the carboxylate and ester carbonyl, which increases regularly from 8 to 11, influences reactivity of catalytic ester cleavage in a way that is decidedly suggestive of the importance of a good match between ester size and metal‐to‐metal distance. However, the superiority of the vicinal catalyst 2 relative to 3 cannot be explained on the basis of the putative match of ester size to intermetal distance, thus providing an indication that additional, still poorly understood effects may contribute significantly to catalytic efficiency.
Structure, Energetics, Electronic, and Hydration Properties of Neutral and Anionic Al3O6, Al3O7, and Al3O8 Clusters
Authors: Gowtham S. , Lau Kah Chun , Deshpande Mrinalini , Pandey Ravindra , Gianotto Anita K. , Groenewold Gary S. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Study of Sialon S‐Phases M2AlxSi12‐xN16‐xO2+x, M: Ba and Ba0.9Eu0.1, by X‐Ray Single Crystal Diffraction, X‐Ray Powder Diffraction, and Solid‐State Nuclear Magnetic Resonance.
Authors: Esmaeilzadeh S. , Grins J. , Shen Z. , Eden M. , Thiaux M. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Molluscicidal Activity of (2‐Substituted)‐spiro(cyclohexane)‐1′,5‐[1,3,4]oxa/thiadiazolo [3,2‐c]thiazolines.
Authors: Nizamuddin Nizamuddin , Singh A. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Enantioselective Iodolactonization Catalyzed by Chiral Quaternary Ammonium Salts Derived from Cinchonidine.
Authors: Wang Mang , Gao Lian Xun , Mai Wen Peng , Xia Ai Xiang , Wang Fang , Zhang Suo Bo 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Palladium‐Catalyzed Enantioselective 1,3‐Rearrangement of Racemic Allylic Sulfinates: Asymmetric Synthesis of Allylic Sulfones and Kinetic Resolution of an Allylic Sulfinate.
Authors: Jagusch Thomas , Gais Hans‐Joachim , Bondarev Oleg 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Allyltriphenylphosphonium Peroxodisulfate (CH2=CHCH2PPh3) 2S2O8: An Efficient and Convenient Reagent for the Oxidation of Alcohols and Silyl and THP‐Ethers under Nonaqueous Conditions.
Authors: Tajbakhsh M. , Lakouraj M. M. , Fadavi A. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Copper Sulfate‐Pentahydrate‐1,10‐phenanthroline Catalyzed Amidations of Alkynyl Bromides. Synthesis of Heteroaromatic Amine Substituted Ynamides.
Authors: Zhang Yanshi , Hsung Richard P. , Tracey Michael R. , Kurtz Kimberly C. M. , Vera Eymi L. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Highly Stereoselective Synthesis of (1E)‐2‐Methyl‐1,3‐dienes by Palladium‐Catalyzed trans‐Selective Cross‐Coupling of 1,1‐Dibromo‐1‐alkenes with Alkenylzinc Reagents.
Authors: Zeng Xingzhong , Qian Mingxing , Hu Qian , Negishi Ei‐ichi 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Environmentally Benign Processes for Making Useful Fluorocarbons: Nickel‐ or Copper(I) Iodide‐Catalyzed Reaction of Highly Fluorinated Epoxides with Halogens in the Absence of Solvent and Thermal Addition of CF2I2 to Olefins.
Authors: Yang Zhen‐Yu 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Double Nucleophilic Addition of Azide and Tetraallyltin of the Latent α,β‐Unsaturated Aldehydes Using in situ Hydrolysis of the Imino Functionality Promoted by Tin(IV) Chloride Pentahydrate.
Authors: Shimizu Makoto , Nishi Takafumi 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Asymmetric Synthesis of the Carbocyclic Nucleoside Building Block (R)‐(+)‐4‐Aminocyclopentenone Using δ‐Amino β‐Ketophosphonates and Ring‐Closing Metathesis (RCM).
Authors: Davis Franklin A. , Wu Yongzhong 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Rapid and Highly Chemoselective Biomimetic Oxidation of Organosulfur Compounds with Tetrabutylammonium Peroxymonosulfate in the Presence of Manganese meso‐Tetraphenylporphyrin and Imidazole.
Authors: Iranpoor Nasser , Mohajer Daryoush , Rezaeifard Abdol‐Reza 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Access to New 2‐Oxofuro[2,3‐b]pyrroles and 2‐Methylenepyrroles Through the Reaction of 1,2‐Diaza‐1,3‐butadienes and γ‐Ketoesters.
Authors: Attanasi Orazio A. , De Crescentini Lucia , Favi Gianfranco , Filippone Paolino , Mantellini Fabio , Santeusanio Stefania 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
From 1,4‐Diketones to N‐Vinyl Derivatives of 3,3′‐Bipyrroles and 4,8‐Dihydropyrrolo[2,3‐f]indole in Just Two Preparative Steps.
Authors: Trofimov Boris A. , Zaitsev Alexey B. , Schmidt Elena Yu. , Vasil'tsov Alexander M. , Mikhaleva Al'bina I. , Ushakov Igor' A. , Vashchenko Alexander V. , Zorina Nadezhda V. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Nonracemic 9‐(1‐Methoxycarbonylethyl)‐1,2,3,4‐tetrahydrocarbazole.
Authors: Kurkin A. V. , Nesterov V. V. , Karchava A. V. , Yurovskaya M. A. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Rearrangement of Cyclic Allenes via 1,2‐Dehydro[10]annulenes: Intramolecular Dehydro Diels—Alder of 3‐(Trimethylsilyl)propiolamides.
Authors: Rodriguez David , Castedo Luis , Dominguez Domingo , Saa Carlos 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Methanesulfonamide Group at Position‐4 of the C‐5‐Phenyl Ring of 1,5‐Diarylpyrazole Affords a Potent Class of Cyclooxygenase‐2 (COX‐2) Inhibitors.
Authors: Singh Sunil Kumar , Vobbalareddy Saibaba , Shivaramakrishna Samala , Krishnamraju A. , Rajjak Shaikh Abdul , Casturi Seshagiri Rao , Akhila Vangoori , Rao Yeleswarapu Koteswar 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Mild and Efficient Procedure for the Synthesis of 1‐(Alkyldithiocarbonyl)‐imidazoles.
Authors: Wang Yi‐Qiang , Ge Ze‐Mei , Hou Xue‐Ling , Cheng Tie‐Ming , Li Run‐Tao 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Clean One‐Pot Synthesis of Tetrahydrobenzo[b]pyran Derivatives Catalyzed by Hexadecyltrimethyl Ammonium Bromide in Aqueous Media.
Authors: Jin Tong‐Shou , Wang Ai‐Qing , Wang Xin , Zhang Jian‐She , Li Tong‐Shuang 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Clean Synthesis of 1,8‐Dioxo‐octahydroxanthene Derivatives Catalyzed by p‐Dodecylbenzenesulfonic Acid in Aqueous Media.
Authors: Jin Tong‐Shou , Zhang Jian‐She , Xiao Jin‐Chong , Wang Ai‐Qing , Li Tong‐Shuang 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Simple and Convenient Synthesis of 4,6‐Disubstituted 3‐Cyanopyridin‐2(1H)‐ones under Solvent‐Free Microwave Conditions.
Authors: Dave Chaitanya G. , Shah Dhaval A. , Agrawal Yadvendra K. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
A New Synthetic Method of 1,4‐Dihydro‐2H‐3,1‐benzoxazin‐2‐ones: Selenium‐Catalyzed Reductive Carbonylation of Aromatic Nitro Compounds with Carbon Monoxide.
Authors: Nishiyama Yutaka , Naitoh Yoshitaka , Sonoda Noboru 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
New 1,3‐Dihydroazepin‐2‐one Derivatives by [3,3]‐Sigmatropic Rearrangement of Suitably Substituted 2‐Alkenylcyclopropyl Isocyanates.
Authors: Reissig Hans‐Ulrich , Boettcher Gesine , Zimmer Reinhold 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Chromophoric Macrocycles from the Oxidation of Bis(aminofurazanylic) Ethers of 1,2‐Diols.
Authors: Sheremetev Aleksei B. , Shatunova Elena V. , Averkiev Boris B. , Dmitriev Dmitrii E. , Petukhov Viktor A. , Antipin Mikhail Yu. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Part 1. Efficient Strategies for the Construction of Variably Substituted Bicyclo[5.3.1]undecenones. (AB Taxane Ring Systems).
Authors: Laurent Alain , Villalva‐Servin Nidia P. , Forgione Pat , Wilson Peter D. , Smil David V. , Fallis Alex G. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 3α,7α,14α‐Trihydroxy‐5β‐cholan‐24‐oic Acid: A Potential Primary Bile Acid in Vertebrates.
Authors: Kakiyama Genta , Iida Takashi , Goto Takaaki , Mano Nariyasu , Goto Junichi , Nambara Toshio 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Solvent‐Free Cyclocondensation of Lactim Ethers with Anthranilic Acid under Microwave Irradiation.
Authors: Cledera Pilar , Sanchez J. Domingo , Caballereo Esmeralda , Avendano Carmen , Ramos M. Teresa , Menendez J. Carlos 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Evaluation of Analogues of S‐Adenosyl‐L‐methionine, as Inhibitors of the E. coli Cyclopropane Fatty Acid Synthase.
Authors: Guerard Christine , Breard Maud , Courtois Fabienne , Drujon Thierry , Ploux Olivier 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Carminomycin, 14‐Hydroxycarminomycin and Its Novel Carbohydrate Derivatives Potently Kill Human Tumor Cells and Their Multidrug Resistant Variants.
Authors: Tevyashova Anna N. , Shtil Alexander A. , Olsufyeva Eugenia N. , Simonova Valeria S. , Samusenko Alexei V. , Preobrazhenskaya Maria N. 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
SF2809 Compounds, Novel Chymase Inhibitors from Dactylosporangium sp. Part 1. Taxonomy, Fermentation, Isolation and Biological Properties.
Authors: Tani Masato , Gyobu Yasuhiro , Sasaki Toru , Takenouchi Osami , Kawamura Takashi , Kamimura Takashi , Harada Toshiaki 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Biological Activities of Methyl‐4‐[[(2E)‐3,7‐dimethyl‐2,6‐octadienyl]oxy]‐3‐hydroxybenzoate.
Authors: Baek Seung Hwa , Oh Hyun Ju , Lim Jin A. , Chun Hyun Ja , Lee Hyun Ok , Ahn Jong Woong , Perry Nigel B. , Kim Hyung Min 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Nanoparticles Produced by Laser Ablation of HfS3 in Liquid Medium: Inorganic Fullerene‐Like Structures of Hf2S.
Authors: Nath Manashi , Rao C. N. R. , Popovitz‐Biro Ronit , Albu‐Yaron Angi , Tenne Reshef 
Published: July 2004

AbstractFor Abstract see ChemInform Abstract in Full Text.
Diagnostic limitations in testicular cytopathology: To what extent is fine‐needle aspiration reliable for the diagnosis of epidermoid cyst of the testis?
Authors: Pérez‐Guillermo Miguel , García‐Solano José , Sánchez‐Sánchez Carlos , Montalbán‐Romero Socorro , Acosta‐Ortega Jesús 
Published: July 2004

AbstractThis article describes the cytologic and histologic findings of a epidermoid cyst of the testis diagnosed by means of fine‐needle aspiration (FNA) cytology. The gross and cytologic features are creamy aspirate, squamous cells, squamae, and fragments of granulomatous tissue. The cytologic features are fairly typical and similar to those observed in cutaneous epidermoid cysts; however, in this setting, the differential diagnosis should be carried out mainly with teratoma and dermoid cysts. The patient's age and precise location of the mass are paramount in the differential diagnosis. We believe that FNA is a reliable tool for the diagnosis of testicular epidermoid cysts, but the differential diagnosis with dermoid cysts should be based on histology. Diagn. Cytopathol. 2004;31:83–86. © 2004 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of bilateral renal malakoplakia
Authors: Gupta Mamta , Venkatesh Sudhakar K. , Kumar Anant , Pandey Rakesh 
Published: July 2004

AbstractIsolated bilateral renal malakoplakia in the absence of concomitant involvement of the urinary tract is a rare occurrence. We report imaging, cytologic, and histologic findings of such a case diagnosed initially by fine‐needle aspiration (FNA) cytology. A 26‐yr‐old female presented with pain in the left flank, fever, anorexia, and weight loss for the past 2 mo. A left lumbar lump was palpable on physical examination. Imaging studies showed an enlarged nonfunctioning left kidney and a small lesion in the right kidney with preserved function. FNA from both kidneys yielded purulent material positive for E. coli on culture. The smears were inflammatory, with a predominance of neutrophilic polymorphs and numerous histiocytes along with some intracellular and extracellular Michaelis‐Guttman bodies, which were highlighted with the use of a PAS stain. Histology of the nephrectomy specimen showed ill‐defined nodules, composed of foamy histiocytes intermingled with neutrophils, plasma cells, and many variably sized concentric laminated bodies. The right‐side lesion resolved with the use of broad‐spectrum antibiotics in conjunction with ascorbic acid and bethanecol. When imaging studies are suggestive of a chronic inflammatory process, renal malakoplakia must always be considered in the differential diagnosis even if aspirated material shows a predominance of polymorphonuclear leukocytes. The use of special stains like PAS, Von Kossa, and Perl's helps in reaching the correct diagnosis. Diagn. Cytopathol. 2004;31:116–117. © 2004 Wiley‐Liss, Inc.