bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in microcirculation variables in an acute endotoxaemic equine model
Authors: Sauter Philipp K. , Steblaj Barbara , Kästner Sabine B. R. , Söbbeler Franz J. , Reiners Julia K. , Kutter Annette P. N. , Bautitsta Alvaro J. Gutiérrez , Neudeck Stephan 
Published: January 2025

AbstractBackgroundMicrocirculation is the essential link between macrocirculation and cellular metabolism.ObjectivesTo test our hypotheses that microcirculation variables will show a heterogeneous flow pattern during experimental endotoxaemia, and that fluid therapy and noradrenaline (NA) infusion will normalise altered microcirculation variables.Study designIn vivo experiments.MethodsSix healthy adult horses were anaesthetised with dexmedetomidine, ketamine, and diazepam and were mechanically ventilated under isoflurane anaesthesia. Endotoxaemia was induced with 30 ng kg−1 Escherichia coli lipopolysaccharide intravenously. One hundred and twenty minutes later fluid bolus and noradrenaline (NA) infusion were administered to produce normotension. Pulse rate (PR) and mean arterial blood pressure (MAP) were measured and microcirculation variables were obtained by side‐stream darkfield technique (de Backer density (DBD), perfused de Backer density (PDBD), proportion of perfused vessels, microvascular flow index (MFI), heterogeneity index (HI)), laser Doppler flowmetry (blood flow) and white light spectrometry (tissue oxygen saturation (tSO2)) in sublingual, jejunal and genital area. Measurements were obtained at baseline, after endotoxin, at 60 and 120 min and during the normotensive phase. Data were analysed by mixed model variance analysis and Tukey–Kramer.ResultsThe PPV decreased significantly over time by 30% (p < 0.001) at the jejunum. MFI decreased from baseline to ET60 and from baseline to ET120 in sublingual and genital mucosa (2.9 vs. 1.4, p < 0.001 and 2.8 vs. 1.9, p < 0.01), respectively. The sublingual HI increased from baseline to ET60, ET120 and NA (0.1 vs. 0.9, p = 0.02; vs. 0.6, p = 0.01; vs. 0.3, p = 0.01), respectively. The genital HI increased from baseline to ET120 (0.2 vs. 1.1, p ≤ 0.01) and NA (0.16 vs. 0.53, p < 0.05, respectively). Moderate agreement between observers for MFI assessment was present (kappa = 0.4). The PR significantly increased, and MAP significantly decreased from baseline over time.Main limitationsThe obtained data could be influenced by secretions, pressure artefacts, the experience of the examiner and the sampling location. Blood flow was not quantified and there was no control group.ConclusionsOverall, short‐term experimental endotoxaemia did negatively alter MFI and HI; however, it did not alter tSO2, blood flow, DBD, PDBD or proportion of perfused vessels. Intravenous fluid therapy and NA did not restore MFI and HI to baseline values.
Senolytic Targeting of Anti‐Apoptotic Bcl Family Increases Cell Death in UV‐Irradiated Senescent Melanocytes: Search for Senolytics
Authors: Kim Jin Cheol , Kim Na Yeon , Kim Yeongeun , Baek Du Jin , Park Tae Jun , Kang Hee Young 
Published: January 2025

ABSTRACTSenescent melanocytes have been suggested to play a role in the development of ageing‐associated pigmentary changes and skin ageing. Here, we assessed the senolytic capacity of recognised senolytic chemicals and natural compounds in UV‐irradiated senescent melanocytes. Among the tested agents, only ABT‐737 and ABT‐263 showed a significant reduction in the number of SA‐β‐Gal‐positive senescent melanocytes and in the expressions of p16INK4A and p21Waf1. The senolytic effects of the ABT drugs were associated with increased expression of cleaved caspase‐3, which was hindered with a caspase inhibitor, Z‐VAD. These findings indicate that ABT‐737 and ABT‐263 eliminate senescent melanocytes through caspase‐mediated apoptosis, suggesting their future potential to address ageing skin.
Flying Fish Habitat and Co‐Occurrence With Seabirds in the Northern Gulf of Mexico
Authors: Michael Pamela E. , Haney J. Christopher , Gleason Jeffrey S. , Hixson Kathy M. , Satgé Yvan G. , Jodice Patrick G. R. 
Published: January 2025

ABSTRACTFlying fish (family Exocoetidae) play an important role in marine food webs, linking sub‐surface and aerial predators. The association of seabirds with sub‐surface predators in subtropical and tropical regions through facilitated foraging events is a well‐known phenomenon and is sometimes used to identify fishing grounds for flying fish, flying fish roe, and tunas. In the northern Gulf of Mexico (nGoM), few studies have assessed flying fish distribution, and none have directly evaluated flying fish–seabird co‐occurrence. Using vessel‐based observations of surfacing flying fish flights, we characterized the distribution of flying fish and their co‐occurrence patterns with seabirds in the nGoM. We modeled the distribution and relative density of flying fish flights using Generalized Additive Models. We then assessed co‐occurrence patterns of flying fish with all seabird species seen in the area, encompassing the footprint of flying fish detections. Flying fish were detected across the U.S. Exclusive Economic Zone, with flight densities greater on the mid‐continental shelf and into pelagic waters south of Louisiana, and greater flight densities were associated with regionally low chlorophyll‐a and warm water. Flying fish flights were greatest in spring months through early fall months. Sooty terns (Onychoprion fuscatus), considered near‐obligate commensals with tuna, contributed a much higher percent of the cumulative density of the seabirds co‐occurring with versus without flying fish. Flying fish could be an ecological attractant for high abundances of visually conspicuous sooty terns, the presence of which may lead to the formation of ephemeral facilitated foraging events consisting of mixed‐species seabird flocks.
Creating the 5A Nursing Response Model for the Emerging Pandemic: A Qualitative, Theory‐Generating Study
Authors: Rim Dahae , Shin Hyunsook , Jeon Hyejin , Lee Young‐Me , Shon Soonyoung , Shim Kaka , Kim Kyung Mi , Simonovich Shannon D. 
Published: January 2025

ABSTRACTAimThe aim of this qualitative exploratory study aimed was to explore how nurses experienced and responded to the COVID‐19 pandemic crisis in clinical settings to develop a response model.DesignFocus group interviews.MethodsThirteen focus group interviews (n = 50) with nurses and nurse leaders practicing in diverse clinical settings were conducted between May and July of 2021 in South Korea. A content analysis was utilised to analyse and interpret the transcribed texts of the recorded focus group interviews.ResultsFive main themes emerged about nurses' responses through the progression of the COVID‐19 pandemic: (1) Alarm, (2) Assessment, (3) Adaptation, (4) Amplification, and (5) Affect, forming a 5A nursing response model. The first four themes describe a time‐ordered sequence, while the fifth theme describes the fluctuations in nurses' affect over time.ConclusionsOur findings explain how nurses deal with an unknown crisis, innovate and lead in the middle of that crisis, and provide a valuable theoretical foundation for developing the 5A response model to prepare for future pandemics. Understanding these core response experiences is vital to preventing the breakdown of the medical system and improving the quality of care provided in emergencies in hospitals and communities.Implications for the Profession (Or Patient Care)Nursing professionals experienced significant emotional upheaval during the pandemic as they continuously adapted to an evolving situation. They assessed the circumstances, addressed emerging challenges, and broadened their nursing competencies to meet unprecedented demands.ImpactThe 5A response model could serve as a blueprint for nurses to effectively respond to unknown crises, innovate, and lead in the middle of a crisis in the future.Reporting MethodWe used the COREQ guidelines.Patient or Public ContributionNo patient or public contribution.
DNA hypomethylation‐related expression of hsa‐miR‐184 contributes to invasive growth of gonadotroph neuroendocrine pituitary tumors
Authors: Tsegaye Biniyam , Kober Paulina , Mossakowska Beata Joanna , Baluszek Szymon , Maksymowicz Maria , Buchalska Barbara , Kunicki Jacek , Bujko Mateusz 
Published: January 2025

AbstractGonadotroph neuroendocrine pituitary tumors are among the most common intracranial neoplasms. A notable proportion of these tumors is characterized by invasive growth which hampers the treatment results and worsens prognoses of patients. Increased hsa‐miR‐184 expression was observed in invasive as compared to non‐invasive gonadotroph tumors. This study aimed to determine the role of hsa‐miR‐184 expression in invasive growth of gonadotroph tumors. QRT‐PCR and bisulfite pyrosequencing were used for evaluating hsa‐miR‐184 expression and MIR184 DNA methylation levels, respectively, in tumors and normal pituitary samples. LβT2 and αT3‐1 gonadotroph cells were used to test the effect of miR‐184 on cell viability (MTT test), proliferation (BrdU incorporation), and migration (scratch assay). RNA sequencing was applied for transcriptome profiling in miR‐184‐treated and untreated LβT2 cells. Differential genes expression analysis combined with target prediction served for identification of miR‐184 targets. MiRNA‐mRNA interaction was subsequently validated with Luciferase reporter assay. Analysis of tissue samples showed that hsa‐miR‐184 is upregulated in gonadotroph tumors and its expression is higher in invasive than in noninvasive ones. Promoter of MIR184 is demethylated in tumors, and the methylation level is negatively correlated with hsa‐miR‐184 expression. Transfecting LβT2 and αT3‐1 with miR‐184 mimic resulted in increased cellular proliferation and viability. Differentially expressed genes were identified when comparing miR‐184‐treated and untreated cells, including Nus1 as the only predicted miR‐184 target. The interaction between miR‐184 and 3'UTR of Nus1 was confirmed in vitro in both LβT2 and αT3‐1. Overexpression of Nus1 resulted in lowering cell viability in both cell lines and proliferation in LβT2. The expression level of NUS1 was lower in invasive than in noninvasive tumors. Our results indicate that DNA hypomethylation‐related increase of hsa‐mir‐184 expression contributes to invasive growth of gonadotroph pituitary tumors through targeting NUS1, being one of the various molecular mechanisms involved in conferring aggressive growth potential.
Severe Liver‐Related Outcomes in Patients With Hepatitis Delta: Results From a Multi‐Ethnic Multicenter Long‐Term Follow‐Up Study
Authors: Furquim d’Almeida Arno , Ho Erwin , Govaerts Liesbeth , Michielsen Peter , Sersté Thomas , Bourgeois Stefan , Delwaide Jean , Moreno Christophe , Orlent Hans , Van Vlierberghe Hans , de Galocsy Chantal , Peeters Michael , Padalko Elizaveta , Van Gucht Steven , Vanwolleghem Thomas 
Published: January 2025

ABSTRACTHepatitis B virus (HBV)–hepatitis delta virus (HDV) coinfection is the most severe form of chronic viral hepatitis, but the factors that determine disease progression and severity are incompletely characterised. This long‐term follow‐up study aims to identify risk factors for severe liver‐related outcomes. In this multicentre national cohort study, data from admission until the last visit between 2001 and 2023 was retrospectively collected from 162 HBV‐HDV coinfected patients. The inclusion criteria were HBsAg or HBV DNA positivity, anti‐HDV or HDV RNA positivity, and at least one follow‐up visit. The median follow‐up was 6.2 years (IQR 3.3–10.2). At baseline, 68/152 (44.7%) patients were diagnosed with advanced liver fibrosis. Forty patients (24.7%) had at least one severe liver‐related outcome during follow‐up. HDV viremia was detectable in 92 patients (64.3%) at last evaluation and was more frequently detectable in patients of European origin (p < 0.001). HDV RNA‐positive patients had a 4.7‐fold higher risk for severe liver‐related outcomes (p < 0.001) and were more frequently diagnosed with advanced fibrosis at baseline (p = 0.007) compared to HDV RNA‐negative patients. Multivariate analyses identified HDV RNA positivity, as well as several markers for liver disease severity, such as INR, platelet count, and advanced fibrosis at baseline, and age at admission as independent risk factors for severe liver‐related outcomes. In conclusion, almost one in four HBV‐HDV coinfected patients developed a severe liver‐related outcome during follow‐up. Several markers for liver disease severity and HDV RNA positivity were the strongest predictors for outcomes.
Echocardiographic evaluation of the size of the main pulmonary artery and right pulmonary artery in dogs with pulmonary hypertension
Authors: Grosso Giovanni , Tognetti Rosalba , Domenech Oriol , Della Pina Andrea , Marchesotti Federica , Patata Valentina , Vezzosi Tommaso 
Published: January 2025

AbstractBackgroundEvaluating the size of the pulmonary artery (PA) is key for the echocardiographic assessment of pulmonary hypertension (PH) in dogs.Hypothesis/ObjectivesTo compare the diagnostic accuracy of the main PA (MPA) and right PA (RPA) sizes for the echocardiographic detection of PH in dogs, and to evaluate differences between precapillary and postcapillary PH dogs.AnimalsFour hundred four dogs; 136 controls and 268 with PH.MethodsProspective, multicenter, observational study. The MPA, maximum and minimum RPA diameter were normalized to body weight (MPA_N, RPAmax_N, and RPAmin_N). The MPA was also indexed to the ascending aorta (MPA/AO), while the RPA size was indexed to the aortic annulus (RPAmax/Aod and RPAmin/Aod). The right pulmonary artery distensibility index (RPADi) was also calculated. The diagnostic accuracy of PA parameters for PH was assessed through the receiver operating characteristic curve analysis and area under the curve (AUC). Measurement variability was assessed trough the coefficient of variation (CV).ResultsThe RPADi, RPAmin_N, and RPAmin/Aod showed similar diagnostic accuracy for the detection of PH (AUC = 0.975, AUC = 0.971, and AUC = 0.953, respectively), higher than MPA/AO (AUC = 0.926), MPA_N (AUC = 0.880), RPAmax_N (AUC = 0.814), and RPAmax/Aod (AUC = 0.803; P < .05). Aside from RPAmax variables, no differences were found between precapillary and postcapillary PH. RPA size parameters showed lower CVs in comparison to MPA/AO and RPADi.Conclusions and Clinical ImportanceAlthough MPA/AO showed an excellent sensitivity and specificity for the detection of PH, the RPAmin exhibited a higher diagnostic accuracy and less measurement variability, thus could represent a new useful parameter for the detection of PH in dogs.
Molecular Epidemiology and Antifungal Susceptibility Profile of Candidozyma Isolates From Argentina
Authors: Taverna Constanza Giselle , Córdoba Susana , Haim Maria Sol , Lombardo Micaela , Vivot Matías Ezequiel , Arias Bárbara Abigail , Vivot Walter , Szusz Wanda , Abbey Darren , Poklépovich Tomas Javier , Canteros Cristina Elena 
Published: January 2025

ABSTRACTBackgroundEpidemiological surveillance of Candidozyma sp. has become important because many species of this new genus have been reported to be responsible for nosocomial outbreaks and to exhibit elevated minimal inhibitory concentrations (MIC) to one or more classes of antifungal drugs.ObjectivesTo describe the genetic relationships among Argentinian clinical isolates belonging to the Candidozyma genus and to study the molecular mechanisms associated with antifungal resistance.MethodsWe performed whole‐genome sequencing of 41 isolates. Identification was based on ribosomal DNA sequencing and susceptibility testing was determined according to the EUCAST document. Phylogenetic analysis, non‐synonymous mutations in genes associated with antifungal resistance and the presence of copy number variations (CNVs) were investigated.ResultsWe identified 12 Candidozyma haemuli, 11 Candidozyma haemuli var. vulneris, 5 Cz. haemuli/ Cz. haemuli var. vulneris ITS hybrids, 8 Candidozyma duobushaemuli and 5 Candidozyma cf. pseudohaemuli. Phylogenetic analysis, together with clinical data, demonstrated nosocomial transmission events. In addition, Cz. haemuli and Cz. haemuli var. vulneris were not separated in the phylogenetic tree; the Cz. cf. pseudohaemuli isolates clustered distantly from the Cz. pseudohaemuli type strain. Most isolates were resistant to amphotericin B, and two Cz. haemuli isolates showed fluconazole resistance and Y132F mutation in ERG11. We did not find CNV in genes associated with antifungal resistance.ConclusionsThese findings highlight the need for epidemiological surveillance of these species and the study of molecular mechanisms associated with antifungal resistance. Furthermore, we propose a taxonomic revision for Cz. haemuli var. vulneris and Cz. pseudohaemuli based on genomic data.
Revision of subfamily Calonecrinae (Coleoptera: Cucujoidea: Nitidulidae) systematics and natural history of a mysterious group
Authors: Lee Min Hyeuk , Lee Seunghyun , Jelínek Josef , Leschen Richard A. B. , Bai Ming , Yap Sheryl A. , Lee Seunghwan 
Published: January 2025

AbstractCalonecrinae represent a unique but small subfamily of Nitidulidae that is endemic to South Asia. Their habitats, the South Asian lowland forests, are under the imminent threat of degradation, posing a risk of rapid extinction for these species in their specific locales. Despite the looming threat to their existence, our understanding of this enigmatic group remains limited. We conducted an examination of museum and newly collected specimens, alongside a review of the literature, which led to the discovery of a new species, Calonecrus mindanaoensis sp. nov., from Mindanao, Philippines. Additionally, we provide detailed illustrations of various type specimens of the genus Calonecrus Thomson. For the first time, the immature stages of Calonecrinae were described based on Calonecrus jendeki, with detailed photographs including scanning electron microscopy images. Additionally, their natural history was uncovered, revealing that all life stages are uniquely adapted to complete their entire life cycle in sticky sap. We reevaluate the phylogenetic placement of the Calonecrinae based on phylogenetic analyses of mitochondrial genomes. Calonecrinae positioned within the Epuraeinae clade and are phylogenetically related to the epuraeine genus Trimenus. Consequently, this study proposes reclassifying Calonecrinae as Calonecrini stat. nov., an extremely modified tribe within the Epuraeinae. This research significantly broadens our understanding of the ecology of Calonecrini. Along with the taxonomic and phylogenetic results, we provide a solid foundation for the conservation of this group and further research into their unique habitat preferences and evolutionary behaviour.
Effectiveness of Combined Physical and Psychological Interventions on Anxiety and Depression Symptoms in Adult Patients With Chronic Obstructive Pulmonary Disease: a Systematic Review Protocol
Authors: Ramos Sílvia , Gomes Aramid , Rosinhas Ana , Silva Rosa , Goes Margarida , Sampaio Francisco 
Published: January 2025

Objective:
The objective of this review is to evaluate the effectiveness of combined physical and psychological interventions on anxiety and depression symptoms in adult patients with chronic obstructive pulmonary disease (COPD).

Introduction:
By 2030, COPD is expected to be the third-leading cause of death and the seventh in terms of overall health impact, measured in disability-adjusted life years. As with other comorbidities, anxiety and depression disorders influence the prognosis. Combined physical and psychological interventions may have better results than isolated interventions for symptoms of anxiety and depression.

Inclusion criteria:
Studies with adult patients with COPD and anxiety and depression symptoms who have undergone combined physical and psychological interventions will be considered for inclusion. This review will primarily include randomized clinical trials.

Methods:
Articles will be searched in CINAHL, Cochrane Central Register of Controlled Trials, Academic Search Complete, Psychology and Behavioral Sciences Collection (via EBSCOhost), APA PsycINFO, PubMed, Web of Science Core Collection, and Scopus. Two independent reviewers will select the studies and apply the JBI tools for critical appraisal and data extraction. Studies will be pooled in a meta-analysis whenever possible. The χ2 test and I2
statistics will be the standard tools for assessing heterogeneity. Statistical analyses will be carried out using the random effects model. The fixed effects model will be applied if there is low heterogeneity between included studies (I2
< 50, or P ≥ 0.5). The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach will be used to grade the certainty of the evidence, and a Summary of Findings will be presented.

Review registration:
PROSPERO CRD42024550523
Effects of noradrenaline and phenylephrine on cerebral oxygen saturation during cardiopulmonary bypass in cardiac surgery
Authors: Marques Edouard , Couture Etienne J. , Bussières Jean S. , Langevin Stephan , Poirier Paul , Voisine Pierre , Caouette Manon , Brassard Patrice 
Published: January 2025

AbstractCardiopulmonary bypass (CPB) in cardiac surgery is associated with a high risk of postoperative neurological complications. Perioperative use of vasopressors is common to counteract arterial hypotension in this setting. However, use of α‐agonist vasopressors has been associated with cerebral desaturations. Given that reductions in cerebral oxygen saturation () can increase postoperative neurological dysfunction, we aimed to investigate the impact of noradrenaline (NA) and phenylephrine (PE) on during the CPB period of a cardiac surgery in 36 patients scheduled for an elective cardiac surgery. Patients were randomized to the intra‐operative use of either NA or PE. During CPB, mean arterial pressure (MAP) was elevated pharmacologically to predefined thresholds of 60 and 80 mmHg, while CPB flow was kept constant. The values were recorded for 5 min per MAP threshold. The MAP increased adequately between thresholds of 60 and 80 mmHg (NA, 59 ± 3 vs. 81 ± 3 mmHg and PE, 61 ± 4 vs. 81 ± 3 mmHg; P ˂ 0.01). The decreased between pressure thresholds of 60 and 80 mmHg (NA, 70 ± 11 vs. 69 ± 11 mmHg and PE, 64 ± 11 vs. 63 ± 11 mmHg; P ˂ 0.01). Reduction in did not differ between vasopressors. The mean relative decrease in across groups was 2.0% (95% confidence interval: 0.6 to 2.1). Elevation in MAP mediated solely by vasopressors induces significant decreases in during cardiac surgery under CPB. However, their impact on remains clinically non‐significant according to current guidelines.
Study of Deformation Mechanism and Surface Formation of Laser Coupled Ultrasonic Vibration-Assisted Turning of SiCp/Al Composites
Authors: Hao ZhaoPeng , Zhuang XiaoHan , Fan YiHang 
Published: January 2025

Abstract
In order to enhance the machinability of SiCp/Al composites and improve the surface quality after machining, a laser-ultrasonic vibration-assisted machining (LA-UVAM) method is presented. In order to investigate the deformation mechanism in the material and the mechanism of surface formation in LA-UVAM, a finite element model of LA-UVAM was established. Using the secondary development imported into abaqus, the Johnson–Cook principal equations containing strain gradients were programmed into the user subroutine VUMAT for simulation. Combining the simulation and experimental results, the influence of dislocation motion in the temperature, stress, and strain gradient effects on cutting deformation during SiCp/Al composite machining was analyzed from the perspective of material dislocation theory. The results show that the presence of SiC particles changes the microstructure of the matrix material and induces high strain gradients in the matrix. In addition, the damage of the particles is closely related to their relative position to the tool. LA-UVAM significantly improves the surface quality with fewer broken particles and defects on the machined surface compared to the conventional machining method. The simulation results are in good agreement with the cutting experiment results. Therefore, LA-UVAM can be used for the efficient machining of SiCp/Al composites.