bluuebunny/crossref_metadata_2025_split huggingface dataset:
Place‐Based Accentedness Ratings Do Not Predict Sensitivity to Regional Features
Authors: Campbell‐Kibler Kathryn 
Published: February 2025

ABSTRACTDiscussions of sociolinguistic awareness are often about how patterns observed in one practice (often linguistic production) appear in others (often person perception or metalinguistic commentary). Models like Labov's indicator/marker/stereotype trichotomy force this complexity into a single dimension, due to presupposing a conscious/unconscious distinction unsupported in current cognitive psychology. A more effective approach takes a theoretical step back, asking basic questions about how analogous sociolinguistic meanings relate across activities. In this article, I do so by asking whether explicit verbal reports and speaker evaluations of accentedness in Ohio correlate in strength across individual language users. Such a correlation would suggest a shared representation and/or a shared learning process. A total of 1106 participants listened to Ohio talkers reading word lists of trap, dress, lot, or goose tokens. Participants rated each talker's accentedness, then the accentedness of seven Ohio places. The expected main effects emerged: southern and rural Ohio were most accented, then northern Ohio, and lastly cities and central Ohio. Likewise, the acoustic features influenced talker ratings. Crucially, however, these two effects largely did not interact: those most likely to describe northern (southern) Ohio as accented were no more or less sensitive to northern (southern) vowel features. These results support the small but growing evidence that indexical relationships are learned and used independently across linguistic practices. They also move us further from a unidimensional model of awareness toward an approach where different systems are treated independently.
Adaptive Divergence and Functional Convergence: The Evolution of Pulmonary Gene Expression in Amphibians of the Qingzang Plateau
Authors: Chang Liming , Zhu Wei , Chen Qiheng , Zhao Chunlin , Sui Lulu , Shen Cheng , Zhang Qunde , Wang Bin , Jiang Jianping 
Published: February 2025

ABSTRACTThe Qingzang Plateau, with its harsh environmental conditions—low oxygen, high ultraviolet radiation and significant temperature fluctuations—demands specialised adaptations for survival. While genetic adaptations have been extensively studied, gene expression's role in amphibian adaptation to high elevations remains understudied. This study analysed pulmonary gene expression in 119 amphibians across the plateau to explore how genetic and environmental factors shape expression evolution. Transcriptomic analyses revealed significant interspecies variation, driven by environmental factors like temperature, oxygen levels, UVB radiation and precipitation. Principal Component and Mantel analyses found no significant correlation between gene expression divergence and genetic distance. Instead, species‐specific traits and environmental pressures were pivotal in shaping expression patterns. PERMANOVA analysis showed environmental factors had varying impacts on species. For instance, Bufo gargarizans exhibited a strong gene expression response to multiple environmental factors, while Scutiger boulengeri was less influenced, reflecting diverse adaptive strategies. Functional enrichment analysis highlighted convergence in key biological processes, such as energy metabolism, apoptosis and autophagy, despite species‐specific gene expression differences. These processes are critical for surviving the plateau's extremes. The findings suggest that gene expression evolution in amphibians on the Qingzang Plateau is shaped by both genetic diversity and environmental pressures. Although gene expression profiles vary, they converge on essential functions, offering insights into adaptation mechanisms in extreme environments.
Novel Regulators and Their Epistatic Networks in Arabidopsis' Defence Responses to Alternaria alternata Infection
Authors: Zeng Qi , Liu Xifan , Yan Xuemei , Zhang Jiahao , Li Chao , Yan Chengtai , Zhang Yanfeng , Kliebenstein Daniel , Li Baohua 
Published: February 2025

ABSTRACTNecrotrophic pathogens cause serious threats to agricultural crops, and understanding the resistance genes and their genetic networks is key to breeding new plant cultivars with better resistance traits. Although Alternaria alternata causes black spot in important leafy brassica vegetables, and leads to significant loss of yield and food quality, little is known about plant–A. alternata interactions. In this study, we used a unique and large collection of single, double and triple mutant lines of defence metabolite regulators in Arabidopsis to explore how these transcription factors and their epistatic networks may influence A. alternata infections. This identified nine novel regulators and 20 pairs of epistatic interactions that modulate Arabidopsis plants' defence responses to A. alternata infection. We further showed that the glucosinolate 4‐methoxy‐indol‐3‐ylmethyl is the only glucosinolate consistently responsive to A. alternata infection in Col‐0 ecotype. With the further exploration of the regulators and the genetic networks on modulating the accumulation of glucosinolates under A. alternata infection, an inverted triangle regulatory model was proposed for Arabidopsis plants' defence responses at a metabolic level and a phenotypic level.
Alternating inverse modulation of xylem K+/NO3− loading by HY5 and PIF facilitates diurnal regulation of root‐to‐shoot water and nutrient transport
Authors: Jing Si , Zhang Han , Yang Zidan , Du Xin‐Qiao , Hu Yingying , Wang Shao‐Shuai , Wang Shuwei , Zhang Kaina , Li Zhen , Wu Wei‐Hua , Kudla Jörg , Li Jigang , Wang Yi 
Published: February 2025

Summary
Diurnal light–dark cycles regulate nutrient uptake and transport; however, the underlying molecular mechanisms remain largely unknown.
Transcription factor MYB59 and ion transporter NPF7.3 participate in root‐to‐shoot K+/NO3− translocation in Arabidopsis. In this study, transcriptional analyses and western blotting experiments revealed the diurnal expression of the MYB59‐NPF7.3 module. ChIP‐qPCR and EMSA showed that transcription factors HY5 and PIF directly bind to the MYB59 promoter. Phenotype analyses and ion content measurement indicated that HY5 and PIF antagonistically control root‐to‐shoot K+/NO3− translocation through the MYB59‐NPF7.3 module.
We found HY5 proteins accumulate in roots and repress MYB59 transcription during daytime, while PIF proteins promote MYB59 transcription in the dark. The expression levels of the NPF7.3 transcript and protein are gradually decreased during daytime, but increased at night. The enhancement of K+/NO3− loading into the xylem mediated by NPF7.3 could increase root pressure at night, which maintained the root‐to‐shoot water/nutrient translocation.
This study reveals a synergistic mechanism between light signaling and nutrient transport in plants, and defines a diurnal molecular switch of driving forces for root‐to‐shoot water/nutrient translocation.
Population genomics of the gametophyte‐only fern Vittaria appalachiana provides insights into clonal plant evolution
Authors: Pelosi Jessie A. , Sorojsrisom Elissa S. , Barbazuk William Brad , Sessa Emily B. 
Published: February 2025

Summary
How asexually reproducing organisms maintain genetic diversity and adaptive potential is a long‐standing question in evolutionary biology. Asexual lineages have historically been thought of as evolutionary dead ends, yet some exhibit remarkable persistence through time.
The gametophyte‐only fern Vittaria appalachiana is a clonal eukaryote, the focus of extensive study due to its peculiar habit and life history, and is an excellent system to explore the consequences of asexuality. Using reduced representation sequencing and life cycle simulations, we assess theoretical expectations for genomic consequences of long‐term asexual reproduction and test hypotheses about its origin and demographic history.
We show that V. appalachiana colonies are not patches of single genotypes but are mosaics of genetic diversity, and the accumulation of mutations in the absence of recombination plays an important role in driving this diversity. We identify increased genomic variation, excess heterozygosity, decreased population differentiation, and increased effective population size, all of which are consistent with the expectations for prolonged clonality. Our analyses support the hypothesis that the loss of sexual reproduction in V. appalachiana occurred during the Last Glacial Maximum.
Our results from empirical and simulation‐based analyses illuminate how an asexual eukaryote generates, retains, and partitions genomic diversity.
Haplotype‐resolved genome assembly provides new insights into the genomic origin of purple colour in Prunus mume
Authors: Meng Juan , Li Ziwei , Wang Haoning , Miao Runtian , Liu Xu , Miao Dapeng , Zhao Chunxu , Wang Guijia , Cheng Tangren , Zhang Qixiang , Sun Lidan 
Published: February 2025

SummaryPrunus mume, an important ornamental woody plant in the Rosaceae family, contains many interspecific hybridizations. Purple colour is a breeding trait of aesthetic value for P. mume, but little is known about the origin and genetic architecture of this trait. Here we address these issues by producing a haplotype‐resolved genome from an interspecific hybrid cultivar of P. mume (M) and P. cerasifera ‘Pissardii’ (C), named P. mume ‘Meiren’, followed by a detailed molecular characterization. The final length of the diploid genome is 499.47 Mb, with 250.66 Mb of haplotype M (HM) and 248.79 Mb of haplotype C (HC). Approximately 95.42% (476.61 Mb) of the phased assembly is further anchored to 16 homologous chromosomes. Based on the genomic variation, we identify a 1.8 Mb large‐fragmented inversion (INV) on chromosome 1b of HC, which co‐segregates with purple colour traits of ‘Meiren’ inherited from its purple C parent ‘Pissardii’. We find that a MYB transcription factor, PmmMYB10.5b, resides at the distal breakpoint of the INV, which displays consistent allele‐specific expression (ASE). By directly binding to the promoter of anthocyanin synthetic alleles, PmmMYB10.5b serves as a co‐activator to promote anthocyanin accumulation in ‘Meiren’ organs. Notably, the INV identified in ‘Meiren’ is generated from ‘Pissardii’ rather than P. cerasifera, which alters the promoter sequence of PmmMYB10.5b, activates its expression and results in the purple colour trait. Results from this study shed light on the evolutionary origin of purple colour in ‘Meiren’ and could potentially provide guidance on the genetic improvement of colour traits in ornamental woody plants.
Mining of Candidate Novel Alleles Using GWAS and Haplotype Identification for Rice Blast Resistance
Authors: Mohanavel Williams , Ramalingam Ajay Prasanth , Ayyenar Bharathi , Rajagopalan Veera Ranjani , Mohanavel Vignesh , Subburaj Samuthirapandi , Manickam Sudha , Rajasekaran Raghu , Chellappan Gopalakrishnan , Swaminathan Manonmani , Perumal Ramasamy , Muthurajan Raveendran , Prasad P. V. Vara 
Published: February 2025

ABSTRACTBlast caused by Pyricularia oryzae (synonym Magnaporthe oryzae) is a major fungal disease affecting productivity and quality in rice‐growing areas globally. A genome‐wide association study (GWAS) was performed using the blast score(s) and whole‐genome sequence data pertaining to a subset of 280 diverse accessions of the IRRI 3 K Rice Genome panel. Continuous variation was observed, with 8.9% of the total accessions resistant, 39.6% moderately resistant, 37.2% moderately susceptible and 14.2% susceptible, with average disease severity ratings of 0 to 3, > 3 to 5, > 5 to 7 and > 7 to 9, respectively, indicating the polygenic inheritance of the trait. We identified potential accessions in different subpopulations with a resistance disease reaction (IRGC 127151 [subtrop], 127738 [ind2], 132245 [aro] and 127130 [aus]), having the lowest average disease ratings of < 2. GWAS revealed the significant association of 22 quantitative trait nucleotides (QTNs)—20 main effect and two interaction effect—linked to blast resistance that led to the identification of 43 plant defence‐related candidate genes. The haplo‐pheno association analysis of the candidate genes (LOC_Os02g06470, LOC_Os02g06510, LOC_Os07g34370, LOC_Os08g29760, LOC_Os10g07470 and LOC_Os12g34290) associated with the superior haplotypes were identified and play a critical role in defence mechanisms. The identified resistant sources hold promise for stacking the superior haplotypes into an elite genetic background using a haplotype‐based breeding approach for the development of cultivars with blast resistance to a wide range of pathotypes.
Petrography, Mineral Chemistry, and Geochemistry of Calc‐Silicate Skarn Mineralization at Kuh‐e‐Gabri, Rafsanjan, Kerman, Southeastern Iran: A Possible Potential of Industrial Andradite–Wollastonite–Quartz Resource
Authors: Shafizadeh Gholamhossein , Atapour Habibeh 
Published: February 2025

ABSTRACTThe prograde calcic skarn mineralization at Kuh‐e‐Gabri area in Kerman province, Iran, is composed of andradite–grossularite, wollastonite, diopside, hematite, and vesuvianite formed by intrusion of Oligo‐Miocene highly differentiated I‐type granitic stocks into the Cretaceous limestone and conglomerate. The retrograde skarn includes epidote, calcite, hematite, and quartz overprinting on the prograde skarn mineralization. This was followed by impregnations of hydrothermal veins of hematite, quartz, fluorite, and calcite. Supergene oxidation processes led to the occurrence of chrysocolla, dioptase, malachite, and goethite. XRD, DTA, FTIRS, EDXS, XRF, and ICP‐OES data indicate that the garnets are enriched in andradite (22.97%–99.50%)–grossularite (62.50%–75.52%) compositions. The composition of wollastonite is highly depleted in iron, manganese, and hazardous elements and close to pure wollastonite (98.31%–99.79%). The mineral assemblages of quartz–calcite–wollastonite as well as hematite–quartz–calcite–andradite suggest that the prograde skarn formed at about 390°C–500°C under oxygen fugacity of 10−33—10−15 bar. The incompatible element patterns in andradite–grossularite and wollastonite display enrichment in Cs, U, Pb, LREE, and negative Eu anomaly. The occurrence of andradite–grossularite skarn at the Kuh‐e‐Gabri may be the most suitable mineral for abrasive without creating environmental hazards. Acicular wollastonite‐rich skarn may be used for production of ceramics and metallurgical applications. Development of colored andradite, transparent and colored quartz in the skarn and veins may be considered as the valuable resource for the semi‐gem cutting industry. The light‐colored marble is a suitable raw material for metallurgical and refractory applications.
A Comparison of Various Diagenetic Controls by Zeolites on Reservoir Quality: Permian–Triassic Conglomerates, Junggar Basin, Northwestern China
Authors: Zuo Rusi , Zeng Xiang , Cao Zhongxiang , Cai Jingong , Zhang Kuihua , Zhang Guanlong , Sun Zhenmeng 
Published: February 2025

ABSTRACTZeolites are widely distributed in the Permian–Triassic conglomerates of the Junggar Basin and play an important role in reservoir quality. A better understanding of the matching relations between reservoir qualities and zeolitic diagenesis is of great importance for oil exploration in basins. In this study, Permian–Triassic conglomerates from southeast Junggar (SEJ), middle Junggar (MJ), and northwest Junggar (NWJ) were investigated, using casting thin section observations, SEM analysis, EPMA analysis, porosity, and permeability measurements. The Permian–Triassic conglomerates of all three areas have abundant volcanic lithics. The conglomerates from NWJ and MJ contain higher contents of sedimentary lithics and feldspars than those from SEJ. Zeolites of SEJ and NWJ originate from the alteration of volcanic lithics, occurring as pore filling in stage B of eodiagenesis. The zeolitic diagenetic process of SEJ is volcanic lithic–clinoptilolite–analcime–heulandite–laumontite, while that of NWJ is volcanic lithic–analcime. Zeolites of MJ originate from albitization of plagioclase, occurring as pore filling and replacement in stage A of mesodiagenesis. Its diagenetic process is a replacement of plagioclase by laumontite. Diverse zeolitic diagenesis played different roles in reservoir quality. Zeolite cementation and compaction both destroyed the reservoir qualities of SEJ and NWJ. In MJ, the reservoir quality was mainly destroyed by compaction, resulting in strong heterogeneity. In later diagenesis, the dissolution of zeolite cements improved reservoir qualities. SEJ is located farther from hydrocarbon generation sags than NWJ and MJ. As a result, zeolite dissolution pores are poorly developed in SEJ. Previous research has revealed that analcime dissolves more easily than laumontite. Zeolite dissolution pores developed better in NWJ than in MJ. In comparison of the conglomerates with zeolite dissolution pores in the Junggar Basin, NWJ conglomerates have the best reservoir quality, indicating that zeolitic diagenesis can provide implications for oil exploration in the Junggar Basin.
The Public Consequences of Presidential Legitimacy Rhetoric
Authors: Wise Stephanie 
Published: February 2025

ABSTRACTObjectiveThree extant theories alternatively argue that presidential legitimacy rhetoric is highly effective among co‐partisans, largely ineffective due to inattention or misunderstanding, or most likely to backfire and produce a counter‐message effect due to its radicalism. This study explores these theories and extends previous work on the Supreme Court to consider public support for Congress and democratic norms.MethodsAn original survey experiment with 1368 participants is employed.ResultsPresidential legitimacy rhetoric on Congress is largely ineffective but produces a mild backlash effect, particularly among independents. Democratic norm attitudes are distinct from attitudes on the legitimacy of Congress.ConclusionThe results attenuate claims about the power of presidents to upend the legitimacy of major US institutions through rhetoric alone. Support for democratic norms and support for particular US institutions may be distinct attitudes and should not be conflated.
Long-term care insurance and implementation for older people in China: a systematic review of textual evidence protocol
Authors: Yang Xiaoman , Yuan Shuqi , Hu Yan 
Published: February 2025

Objective:
The objective of this systematic review is to synthesize and compare policies and implementation strategies on long-term care insurance for older people in China.

Introduction:
Current information about China’s long-term care insurance from a textual perspective is limited. Due to late inception and fragmented management, textual evidence on this topic will offer a unique perspective on the structure and implementation strategies of long-term care insurance policies in China.

Inclusion criteria:
The population of this review will be adults aged 60 years or over. The phenomena of interest will be long-term care insurance policies and implementation strategies in China. Published sources and gray literature in English and Chinese since 2016 will be included. Exclusions will apply to hospitalized individuals as well as those under 60 years of age, who are permanently disabled due to illness or disability.

Methods:
The proposed systematic review will be conducted in line with the JBI methodology for systematic reviews of textual evidence. Databases to be searched will include PubMed, Embase, (Ovid), CINAHL (EBSCOhost), and China National Knowledge Infrastructure (CNKI). For gray literature, we will conduct an advanced Google search as well as searching ProQuest Dissertations & Theses Global. Additionally, we will search the websites of local governments in pilot cities. The JBI critical appraisal checklist for textual evidence will be used to evaluate the quality of textual sources. All identified sources will be retrieved and assessed in detail by 2 independent reviewers. Data extraction and synthesis will be conducted using the JBI System for the Unified Management, Assessment and Review of Information. A meta-aggregative approach will be employed to synthesize data and conclusions, which will be graded using ConQual. A recommendation will be issued as a Good Practice Statement.

Systematic review registration number:
PROSPERO CRD42023414967
Engaging with patients with diabetes: the role of social media in low-income healthcare organisations
Authors: Cano Andrea , Uddin Mohy , Caceres Fernanda , Rodriguez José , Syed-Abdul Shabbir 
Published: February 2025

BackgroundType 2 diabetes is the fastest-growing global health concern, and its global prevalence is projected to affect 643 million individuals by 2030. Social media platforms, like Facebook, have become crucial channels for healthcare organisations to engage with the public to promote prevention and disease management, especially in low-resource settings like Honduras. This study aims to perform a retrospective analysis of Honduran healthcare organisations’ Facebook posts to understand how effectively they engage diabetes-related content with their followers.MethodsThe top 10 followed healthcare organisations’ Facebook pages were taken as a sample. Data were retrieved from October 2023 to March 2024. Diabetic-related posts were identified using keywords and categorised based on their contents and features.ResultsFindings reveal significant disparities in the frequencies of posts and public engagement among different types of organisations. The majority of posts were classified under the miscellaneous category and text+image feature. Recipes and food-related posts were liked and shared the most among the followers.ConclusionThe results of the study found that patients’ engagement with diabetes-related content was low in social media. The gap between patients’ participation and engagement highlights the need for reassessment and refinement of social media communication strategies for healthcare organisations to empower patients with diabetes through social media and increase public engagement.
Partial transvenous embolization as an intermediate strategy for large brain arteriovenous malformations: A technical note
Authors: Noda Ryuichi , Lima Vinicius Moreira , Iosif Christina , Galon José Eduardo Vitorino , Alabbas Faisal , Saleme Suzana , Rouchaud Aymeric , Mounayer Charbel 
Published: February 2025

BackgroundEndovascular transvenous embolization (TVE) was developed as a curative technique for brain arteriovenous malformations (bAVMs) with small nidi and a single draining vein. We describe a partial transvenous embolization technique (pTVE) as an intermediate embolization session to decrease hemorrhagic complications of larger nidi with multiple venous outlets.MethodWe reviewed our single-center database of bAVMs treated endovascularly between January 2023 and March 2024. Six consecutive patients underwent 10 sessions of pTVE for ruptured AVMs.ResultsThe median age of patients was 24.5 years. The median nidus size was 5 cm (range 4–6 cm). Three patients had grade III lesions according to the Spetzler-Martin classification, two were grade IV, and one was grade V. Four patients had draining veins with multiple efferent veins with each collecting vein, one patient had multiple efferent veins with one collecting vein, and one patient had one efferent vein with a long bifurcation. pTVE achieved partial occlusion in all cases without any hemorrhagic complications. One patient had the AVM totally occluded with two sessions of pTVE. Decision-making was facilitated by creating subcategories of venous angioarchitecture and detailing the technical particularities in the corresponding category.ConclusionWe described a novel treatment technique of transvenous embolization as an adjunct strategy for large bAVMs.