bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gingival lesions in a patient with chronic oral graft‐versus‐host disease: a case report
Authors: Argiriadou Annie S. , Sardella Andrea , Demarosi Federica , Carrassi Antonio 
Published: December 2004

AbstractBackground: The patient reported in this study was diagnosed with acute nonlymphocytic leukemia and underwent an allogenic bone marrow transplantation. She was referred for persisting oral ulceration and pain associated with the transplant procedure.Aim: To present an unusual involvement of gingival tissues during a case of oral chronic graft‐versus‐host‐disease.Conclusion: This is one of the very few reports in the dental literature of a case of oral chronic graft‐versus‐host disease that includes the unusual manifestation of prominent gingival lesions.
Deproteinized cancellous bovine bone (Bio‐Oss®) as bone substitute for sinus floor elevation
Authors: Tadjoedin E. S. , De Lange G. L. , Bronckers A. L. J. J , Lyaruu D. M , Burger E. H. 
Published: December 2004

AbstractObjectives: To study in detail the performance of deproteinized cancellous bovine bone (DPBB, Bio‐Osso®) granules as a bone substitute, a histomorphometric was performed on five patients treated with DPBB for reconstruction of the severely atrophic maxilla.Material and Methods: DPBB was used as mixture with autogenous bone particles, in concentrations that increased from 20% to 100% DPBB, with the time of healing increasing accordingly from 5 to 8 months. A total of 20 vertical biopsies was taken at the time of fixture installation and used for histomorphometry as undecalcified Goldner stained sections.Results: The results show that in all cases, the DPBB granules had been interconnected by bridges of vital newly formed bone. The volume of bone in the grafted area correlated inversely with the concentration of DPBB grafted, and varied between 37% and 23%. However, the total volume of mineralized material (bone plus DPI3B granules) remained within the same range in all five patients (between 53% and 59%). The high values for osteoid and resorption surface, and the presence of tartrate‐resistant acid phosphatase‐positive multinucleated osteoclasts in resorption lacunae, indicated that bone remodeling was very active in all grafts. Osteoclasts were also observed in shallow resorption pits on DPBB surfaces. The percentage DPBB surface in contact with bone remained stable at about 35% and could not be related to the proportion of DPBB grafted.Conclusion: Although the number of patients examined was limited, the data suggest that deproteinized cancellous bovine bone, preferably combined with autogenous bone particles, is a suitable material for sinus floor elevation in the severely atrophic human maxilla.
Change of antibiotic susceptibility following periodontal therapy
Authors: Buchmann Rainer , Müller Rüdiger F. , Van Dyke Thomas E. , Lange Dieter E. 
Published: December 2004

AbstractBackground:  The hypothesis was tested that bacterial susceptibilities in aggressive periodontitis change upon administration of systemic antibiotics as adjuncts to periodontal therapy.Methods:  In 23 subjects (average age 38.9±6.7 years) with aggressive periodontitis, microbial parameters were assessed prior to and 1 year after completion of comprehensive mechanical/surgical and systemic antimicrobial therapy. Following identification of five selected pathogens with the Rapid ID 32 A system, their susceptibilities towards amoxicillin/clavulanate potassium, metronidazole, and tetracycline were examined with the E‐test. Antibiotics were administered according to the test results, and the minimal inhibitory concentrations (MIC90) were reevaluated after 1 year. Statistical analysis was performed on a patient basis, with the site data used for evaluation of the MIC levels.Results:  Bacterial MIC levels remained constant among the three antibiotic treatment groups compared with baseline. Mean MIC90 values ranged from <0.02 to 0.11 μg/ml (amoxicillin/clavulanate potassium), <0.02 to 0.27 μg/ml (metronidazole), and <0.02 to 0.11 μg/ml (tetracycline). Observed changes in susceptibility were attributed to the elimination of single bacterial taxa in the subgingival environment after antibiotic therapy. There were no statistically significant differences in clinical parameters among the treatment groups. Single tetracycline MICs were 1.5‐ to 6‐fold enhanced compared to amoxicillin/clavulanate potassium and metronidazole.Conclusion:  The periodontal pathogens investigated prior to and 1 year after periodontal therapy are tested sensitive to the antimicrobial agents. In aggressive periodontitis, changes in bacterial susceptibility upon the administration of systemic antibiotics are associated with the limited number of isolates tested following therapy.
Gentamicin used as an adjunct to GTR
Authors: Stavropoulos A. , Kostopoulos L. , Mardas N. , Nyengaard J. R. , Karring T. 
Published: December 2004

AbstractObjectives: To evaluate in a discriminating “capsule” model whether local application of gentamicin may have an added effect on bone formation produced by Bio‐Oss® and guide tissue regeneration (GTR).Material and Methods: Thirty male 3‐month‐old Wistar rats were used. After elevation of muscle‐periosteal flaps, a rigid hemispherical Teflon capsule, loosely packed with 0.025 g of Bio‐Oss® impregnated with 2 mg/ml gentamicin sulfate (Garamycin®), was placed with its open part facing the lateral bone surface of the mandibular ramus (test) in one side of the jaw. A capsule filled only with Bio‐Oss® (control) was placed on the contralateral side of the jaw. After healing periods of 1, 2 and 4 months, groups of 10 animals were sacrificed and the specimens were processed for histological examination. The volumes of (1) the space created by the capsule, (2) newly formed bone, (3) Bio‐Oss® particles, (4) loose connective tissue, and (5) acellular space in the capsule were estimated by a point‐counting technique in three to four histological sections of each specimen, taken by uniformly random sampling.Results: The histological evaluation showed limited but increasing bone fill in the capsules from 1 to 4 months in both the test and control sides. After 4 months, the newly formed bone occupied 11.9% (CV: 0.39) of the space created by the capsules at the test sides versus 13.2% (CV: 0.41) at the control sides. There was no statistical significant difference between test and control specimens at any observation time (p>0.05).Conclusion: It is concluded that local application of gentamicin has no added effect on bone formation when combined with Bio‐Oss® and GTR.
The relationship of stress and anxiety with chronic periodontitis
Authors: Vettore M.V. , Leão A.T.T. , Monteiro da Silva A.M. , Quintanilha R.S. , Lamarca G.A. 
Published: December 2004

AbstractAim: This case–control study investigates the relationship of stress and anxiety with periodontal clinical characteristics.Method: Seventy‐nine selected patients (mean age 46.8±8 years) were assigned to three groups in accordance with their levels of probing pocket depth (PPD): control group (PPD≤3 mm, n=22), test group 1 (at least four sites with PPD ≥4 mm and ≤6 mm, n=27) and test group 2 (at least four sites with PPD >6 mm, n=30). An inclusion criterion of the study required that patients presented a plaque index (PI) with a value equal to or larger than 2 in at least 50% of dental surfaces. All subjects were submitted to stress and anxiety evaluations. Stress was measured by the Stress Symptom Inventory (SSI) and the Social Readjustment Rating Scale (SRRS), while the State–Trait Anxiety Inventory (STAI) was used to assess anxiety. Clinical measures such as PI, gingival index (GI), PPD and clinical attachment level (CAL) were collected. Patient's medical history and socioeconomic data were also recorded.Results: The mean clinical measures (PI, GI, PPD and CAL) obtained for the three groups, were: control group, 1.56±0.32, 0.68±0.49, 1.72±0.54 and 2.04±0.64 mm; group 1, 1.56±0.39, 1.13±0.58, 2.67±0.67 and 3.10±0.76 mm, group 2, 1.65±0.37, 1.54±0.46, 4.14±1.23 and 5.01±1.60 mm. The three groups did not differ with respect to percentage of clinical stress, scores of the SRRS, trait and state anxiety. Frequency of moderate CAL (4–6 mm) and moderate PPD (4–6 mm) were found to be significantly associated with higher trait anxiety scores after adjusting for socioeconomic data and cigarette consumption (p<0.05).Conclusions: Based on the obtained results, individuals with high levels of trait anxiety appeared to be more prone to periodontal disease.
Osteogenesis by guided tissue regeneration and demineralized bone matrix
Authors: Mardas N. , Kostopoulos L. , Stavropoulos A. , Karring T. 
Published: December 2004

AbstractAim:  To evaluate in a discriminating capsule model whether bone formation by guided tissue regeneration (GTR) may be influenced by concomitant implantation of demineralized bone matrix (DBM).Materials and Methods:  Thirty 4‐month‐old male albino rats of the Wistar strain were used in the study. Following surgical exposure of the mandibular ramus, a hemispherical, Teflon capsule (5.0 mm in diameter), loosely packed with a standardized amount of DBM, was placed with its open part facing the lateral bone surface of the ramus. At the contralateral side, an empty capsule was placed, serving as control. After healing periods of 15, 30, and 120 days, groups of 10 animals were sacrificed and 40–70 μm thick undecalcified sections of the capsules were produced. In the sections, the cross‐sectional areas of (1) the space created by the capsule, (2) newly formed bone, (3) DBM particles, (4) loose connective tissue as well as the (5) height of the capsules, and (6) that of the newly formed bone were measured.Results:  Increasing bone fill was observed in both test and control sites from 30 to 120 days. After 30 days of healing, the mean amount of bone was approx. 3% of the cross‐sectional area of the capsules at the test sites while it was 8% in the control sites (p<0.05). However, no statistically significant differences were observed between the test (46%) and control (64%) sites after 120 days regarding any of the measured parameters (p>0.05). The newly formed bone in the DBM group at 120 days, on the other hand, appeared more dense than that in the control capsules.Conclusion:  DBM used as an adjunct to GTR did not provide any added effect on bone formation but increased the density of the newly formed bone.
Gingival bleeding on probing increases after quitting smoking
Authors: Nair P. , Sutherland G. , Palmer R. M. , Wilson R. F. , Scott D. A. 
Published: December 2004

AbstractBackground: Gingival inflammation associated with plaque accumulation is delayed or impaired in smokers. Anecdotal evidence suggests that smokers who quit experience an increase in gingival bleeding.Method: A group of 27 subjects on a Quit‐smoking programme were examined for changes in gingival health over a 4–6‐week period.Results: The bleeding on probing with a constant force probe increased from 16% of sites to 32% of sites, despite improvements in the subjects oral hygiene.Conclusion: This provides further evidence that tobacco smoking affects the inflammatory response and that these changes are reversible on quitting.
Modulations of nerve growth factor and Bcl‐2 in ultraviolet‐irradiated human epidermis
Authors: Stefanato Catherine M. , Yaar Mina , Bhawan Jag , Phillips Tania J. , Kosmadaki Maria G. , Botchkarev Vladimir , Gilchrest Barbara A. 
Published: December 2004

Background:  Ultraviolet (UV) irradiation to the skin causes apoptosis of keratinocytes. Melanocytes are more resistant to UV‐induced apoptosis, due, in part, to high levels of antiapoptotic proteins such as Bcl‐2. In vitro studies have shown that nerve growth factor (NGF), a neurotrophic polypeptide, is produced by keratinocytes and exerts a protective role for melanocytes by upregulating Bcl‐2. The purpose of this study was to determine NGF and Bcl‐2 modulations in UV‐irradiated human skin.Methods:  Nine volunteers were irradiated with two minimal erythema doses using solar‐simulated UV irradiation. Seventy‐two hours post irradiation, skin biopsies were obtained from irradiated and sun‐protected skin. The skin specimens were stained with anti‐tyrosinase‐related protein‐1 monoclonal antibody IgG2a (Mel‐5), anti‐Bcl‐2 (monoclonal antibody IgG‐kappa), and with anti‐NGF (polyclonal antibody IgG).Results:  NGF staining was identified within the cytoplasm of epidermal melanocytes, similar to the staining observed for TRP‐1 and Bcl‐2. While no significant difference in the number of TRP‐1‐ and Bcl‐2‐positive melanocytes was observed between irradiated and non‐irradiated skin within 72 h, the number of NGF‐positive melanocytes decreased significantly, 72 h after UV irradiation (p < 0.024). NGF was also identified within keratinocytes, and while non‐irradiated skin exhibited cytoplasmic NGF staining throughout the epidermis, NGF staining was reduced in the lower epidermal layers after UV irradiation.Conclusions:  This is the first in vivo study showing NGF to be present in melanocytes, as well as showing modulations of NGF and Bcl‐2 in melanocytes, following solar‐simulated UV irradiation.
Expression of stratum corneum chymotryptic enzyme in ichthyoses and squamoproliferative processes
Authors: Johnson Brad , Horn Thomas , Sander Christian , Kohler Sabine , R. Smoller Bruce 
Published: December 2004

Objective:  Stratum corneum chymotryptic enzyme (SCCE) is a serine protease, which is thought to play a role in the desquamation of skin via the proteolysis of desmosomes in the stratum corneum. The objective of this study was to investigate the expression of SCCE in ichthyoses and squamoproliferative processes, conditions in which the shedding and replacement of epidermal cells is disrupted.Design:  Tissue samples from cases of Netherton's syndrome, congenital ichthyosiform erythroderma, ichthyosis vulgaris, actinic keratosis, squamous cell carcinoma in situ, and invasive squamous cell carcinoma were examined for expression of SCCE using immunohistochemistry.Main outcome measures:  The slides were qualitatively analyzed for the expression of SCCE by a certified dermatopathologist.Results:  In all disease states, we found that the expression of SCCE was absent in areas of parakeratotic stratum corneum of normal thickness. In areas of mixed orthokeratosis and parakeratosis where the stratum corneum was greatly thickened as might correspond clinically to a cutaneous horn, SCCE staining was either absent or focally aggregated without regard to orthokeratosis or parakeratosis. Of note, complete absence of SCCE expression was not observed in any of the cases of ichthyosis examined, nor was there increased expression of SCCE in the atypical cells of the squamoproliferative disorders.Conclusions:  These results suggest that SCCE is abnormally expressed in skin where epidermal cell kinetics are disrupted due to inherited and acquired defects. Further investigation is needed to determine causality between the abnormal expression of SCCE and the altered cell kinetics in these diseases.
Localized epidermolytic hyperkeratosis of the female external genitalia
Authors: Swann Michael H. , Pujals John S. , Pillow Jessica. , Collier Susannah L . , Hiatt Kim , Smoller Bruce R. 
Published: December 2004

Background:  Epidermolytic hyperkeratosis (EH) is most commonly associated with the diffuse involvement of congenital ichthyosiform erythroderma, but can also be found in a localized pattern. Localized EH is rare, but mucocutaneous lesions have been been identified, most commonly in the mouth.Methods:  We observed a 58‐year‐old African‐American female who noted spots on her genitalia for approximately 2 years. The lesions were increasing in size, darkening, and had become pruritic and sore over the past 6 months.Results:  Physical examination revealed seven scattered, tan to brown, verrucoid papules on the labia and mons pubis, resembling condylomata acuminata or Bowenoid papulosis. Biopsy of a single labial papule revealed epidermal acanthosis, compact hyperkeratotic papillomatosis, perinuclear clear zones, granular keratohyalin clumping, hypergranulosis, and dyskeratosis resulting in intracellular eosinophilic globules, all characteristic of EH.Conclusions:  Because of the rarity of localized genital EH and similar appearance to common diagnoses, clinical confusion may occur without biopsy.
Regional dynamics and dispersal in the vascular plant Silene viscosa (L.) inhabiting small islands
Authors: Löfgren Anders , Jerling Lenn 
Published: December 2004

We investigated and compared the patch occupancy and colonisation and extinction over a 40‐yr period in the vascular plant Silene viscosa in two archipelagos off the Swedish east coast. We also assessed the importance of regional vs local dynamics for regional persistence by examining factors affecting colonisation and extinction processes.

We found an effect of isolation on the patch occupancy in 1957, but this effect disappeared 1997. The effect of isolation was not detected when analysing colonisations and extinctions during the 40‐yr period separately, which suggests that the colonisations and extinctions since 1957 have changed the patch occupancy of S. viscosa. The change in the patch occupancy is attributed to an increased colonisation, presumably mediated by the increase of the greylag goose Anser anser. The large amplitude of population size change, the high persistence of populations during a 40‐yr period, and the long term persistence for some populations all suggest that the distribution pattern approximates a distribution that is expected to be generated from an Island‐Mainland model, where a number of large populations are persistent and a number of smaller populations are more prone to extinction due to catastrophes (nesting cormorants) and environmental stochasticity (summer drought). Most evidence suggests that regional processes are of less importance than local processes for regional persistence on a time scale of 40 yr for S. viscosa.
Validation and clinical implication of a quantitative real‐time PCR determination of MDR1 gene expression: comparison with semi‐quantitative PCR in 101 patients with acute myeloid leukemia
Authors: Olesen L.H. , Nørgaard J.M. , Pallisgaard N. , Bukh A. , Hokland P. 
Published: December 2004

Abstract: Introduction: The multidrug resistance protein 1 (MDR1) has the capacity to extrude chemotherapeutics and has been implicated in treatment failure in acute myeloid leukemia (AML). Previous methods for determination of MDR1 expression have included dye exclusion, demonstration of P‐glycoprotein by flow cytometry and/or immunohistochemistry, and molecular polymerase chain reaction (PCR)‐based assays for RNA expression. However, these assays have either proven difficult to standardize or tedious to perform. We have therefore designed a real‐time quantitative (RQ)‐PCR based assay measuring MDR1 gene expression and validated it in AML patients by direct comparison with a competitive reverse transcriptase polymerase chain reaction (RT‐PCR) assay.Patients and methods: Bone marrow or peripheral blood from 101 AML patients diagnosed (1987–96) at our department were assessed for quantitative expression of MDR1 employing TaqMan RQ‐PCR. These data were compared with results obtained by a semi‐quantitative competitive PCR assay employing an artificial internal RNA construct.Results: While the RQ‐PCR method was able to determine MDR1 gene expression in a continuous fashion over five logs, the semi‐quantitative PCR only yielded data in a discontinuous fashion and over four logs at best. Compared with the MDR1 positive and negative cell lines 8226 DOX40 and REH AML cells exhibited variation of 10 PCR cycles, equivalent to a 1000‐fold difference. A significant correlation was observed between the two methods, Spearman's correlation coefficient = –0.502, P‐value = 10−5.Conclusion: We conclude that, RQ‐PCR is a novel methodology, which enables sensitive and quantitative measurement of MDR1 gene expression. This assay is moreover suitable because of its high throughput for longitudinal follow‐up and large number of patients.
Melanocytic matricoma: case confirmation of a recently described entity
Authors: Williams Christy M. , Bozner Peter , Oliveri Cecilia V. , Horenstein Marcelo G. 
Published: December 2004

Background:  In 1999, Carlson et al. reported two cases of a matrical neoplasm that recapitulates the bulb of the anagen hair follicle, which they designated as melanocytic matricoma.Methods:  Here we report a similar case in a 78‐year‐old white male, who presented with a 0.4 cm purple‐black firm papule in the left preauricular area.Results:  Histologically, the tumor is composed of a dual cell population including admixed epithelial matrical and supramatrical cells with “shadow” cell formation and pigmented dendritic melanocytes. Immunohistochemical studies for cytokeratin highlighted the epithelial component and studies for S‐100 protein, HMB‐45, and vimentin confirmed the melanocytic component. The differential diagnosis considered includes pigmented variants of pilomatricoma, matrical carcinoma, basal cell carcinoma and malignant melanoma.Conclusions:  The case reported herein is the first confirmation of melanocytic matricoma, a distinctive adnexal neoplasm with characteristic clinical and pathologic features, which differentiate it from pigmented pilomatricoma.
Diagnostic imaging of melanocytic skin tumors
Authors: Gerger Armin , Smolle Josef 
Published: December 2004

Background:  In tissue counter analysis, digital images are dissected into subregions (elements), and the digital information in each element is used for statistical analysis. The aim of this study was to test the applicability of tissue counter analysis and CART (Classification and Regression Tree) to the diagnostic discrimination of benign common nevi and malignant melanoma in dermatopathology.Methods:  Two hundred cases each of benign nevi and malignant melanoma were consecutively sampled. CART analyses of background versus tissue elements, cellular versus ‘other’ tissue elements and benign versus malignant cellular elements were performed. For diagnostic assessment, only the percentage of cellular elements suggestive for malignancy in each case was used.Results:  CART analysis led to a correct classification of 99% of background versus tissue elements, 96% of cellular versus ‘other’ tissue elements and 79.1% of benign versus malignant cellular elements. When the percentage of cellular elements suggestive for malignancy in each case was evaluated, 29.5 ± 14% (range 4.1–62.4) ‘malignant’ elements were found in benign nevi (n = 200), in contrast to 75.9 ± 13.9% (range 32.8–97.3) in melanoma (n = 200; z =−16.72, p < 0.001). It turned out that a threshold level of 52.51% provides a correct classification of 192 nevi and 186 melanoma out of 200 each (specificity 96%, sensitivity 93%, positive predictive value 95.9%).Conclusions:  Tissue counter analysis combined with CART may be a useful method for diagnostic purposes in histopathology.