bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved isolation and detection of shiga toxin-producing Escherichia coli O157 and non-O157 from retail imported frozen beef marketed in Saudi Arabia using immunomagnetic separation and multiplex-PCR
Authors: Almulhim Ahlam , Alomar Amer , Alhabib Ibrahim , Yamani Lamya Zohair , Elhadi Nasreldin 
Published: January 2024

Abstract

Shiga toxin-producing Escherichia coli (STEC) and E. coli O157:H7 are responsible for a wide range of foodborne outbreaks worldwide. Both E. coli O157 and non-O157 are causing life-threatening complications, such as hemolytic colitis and hemolytic uremic syndrome, which can result in kidney failure. This study was conducted to determine the presence of both STEC and E. coli O157 in imported frozen beef marketed in the Eastern Province of Saudi Arabia using immunomagnetic beads (IMB), CHROMagr O157 medium, and multiplex-PCR. A total of 201 frozen beef samples were purchased from different supermarkets in the Eastern Province of Saudi Arabia and enriched in tryptic soy broth (TSB). One-milliliter portions from enriched samples in TSB were mixed with immunomagnetic beads (IMB) coated with specific antibodies against E. coli O157 O antigen, separated beads with captured bacteria streaked on CHROMagar O157, and Sorbitol MacConkey (SMAC) agar. The extracted DNA template was examined for potential virulence gene markers (stx-1, stx-2, and eae) using multiplex-PCR. ERIC-PCR DNA fingerprinting was used to identify the clonal relationship among the strains isolated from frozen beef imported from different countries. Among 201 examined frozen beef samples, 88 (43.8%) and 106 (52.7%) were positive for E. coli and produced colorless and mauve colonies on SMAC agar and CHROMagar O157, respectively. Out of 298 isolates in total, 124 and 174 isolates of E. coli were isolated without and with IMB enrichment, respectively. The highest detection rate of virulence gene markers were found among isolates that had been isolated using IMB enrichment, where 25 (8.4%), 2 (0.7%) and 12 (4%) isolates tested positive for stx1, stx2, and eae genes respectively. The least number of virulence gene markers were found among isolates that were isolated without using IMB enrichment with a rate of 12 (4%) and 2 (0.7%) isolates testing positive for stx1 and eae genes, respectively. Among 42 isolates harboring potential virulence gene markers, 11 isolates were identified as E. coli O157 (stx1+/eae+ or stx2+/eae+). ERIC-PCR DNA fingerprinting was able to type the 42 identified isolates of E. coli O157 and E. coli non-O157 into 10 types with four identical related clusters and a genetic similarity rate above 90% homology from the identified isolates. This study confirmed that the imported frozen beef marketed in the Eastern Province of Saudi Arabia harbored virulence gene markers of E. coli O157. IMB and multiplex-PCR methods proved to be more reliable and adequate for detecting shiga toxin-producing E. coli O157 in imported frozen beef.
Optimization-based Tuning Algorithms for Attitude Control of a Delta-wing Unmanned Aerial Vehicle
Authors: Gulivindala Kishore , Kumar Neetesh , Saderla Subrahmanyam , Kim Yoonsoo 
Published: January 2024

Abstract
In the realm of commertial and open source Unmanned Aerial Vehicles(UAVs) flight controllers, extensive outdoor trails and repeated flight tests of UAVs with unknown handelling qualities, poses a great challenge for the process of manual controller tunning. In the current manuscript the aformentioned problem is addressed by proposing a novel optimization-based controller gain estimation method. The proposed method relies on output error formulation of maximum likelihood costfunction and the respective minimization is carried by using two different techniques namely Gauss-Newton optimization and particle swarm optimisation (PSO). The attitude stabilisation of a delta wing UAV is achieved by adopting a Proportional-Integral (PI) and feedforward (FF)-based controller architecture. The PI and FF gains are estimated using both methods and the corresponding results obtained are compared. The estimated gains assisted the controller for accurate tracking of the desired attitude for both simulated and flight test data. It is observed that the results obtained by the proposed method using PSO technique has root mean square error less than 1 deg for pitch and roll angles and 2 deg for yaw angles respectively, which is 28% less compared to gradient based gauss newton optimization method.
Prognostic assessment of patients with parotid gland carcinoma: a nomogram based on coagulation-related factors
Authors: Jiang Wen-Mei , Li Guoli , Xiao Guo-Ming , Li Rui-Yu , Deng Honghao , Yang Si-Wei , Zhang Quan , CHEN YANFENG 
Published: January 2024

Abstract

Objectives This study aimed to construct an effective nomogram based on the clinical and laboratory characteristics to predict the prognosis of patients with parotid gland carcinoma. Methods A total of 125 patients diagnosed with parotid gland carcinoma from Sun Yat-sen University Cancer Center (1999–2016) were retrospectively analyzed, including clinical and pathological data. Overall survival (OS) was the primary endpoint, and it was estimated using the Kaplan–Meier method and the Cox proportional hazard regression model. The prediction nomogram was constructed based on the clinicopathological factors and coagulation indices, and then evaluated using the concordance index. Results Activated partial thromboplastin time, international normalized ratio, and fibrinogen were risk factors for OS (allP < 0.05), and their comprehensive influence on OS was identified by coagulation scores (CS). The tumor grade, neck dissection, and CS emerged as independent factors for OS according to the multivariable analyses. Based on the nomogram, patients were stratified into low- and high-risk groups and significant differences in OS were found between these groups (P < 0.0001). The concordance index of this nomogram was 0.762 (SE = 0.035), higher than that of the T-stage (C-index = 0.652; SE = 0.039) and N-stage (C-index = 0.663; SE = 0.043). Conclusions We established and validated a prognostic nomogram including coagulation factors. The proposed nomogram presented a better prediction ability for the survival of patients with parotid gland carcinoma.
LAMP2A regulates cisplatin resistance in colorectal cancer through mediating autophagy
Authors: Shi Zhi-Liang , Yang Shuting , Shen Chenglong , Shao Jiazhe , Zhou Fang , Liu Haichen , Zhou Guo-Qiang 
Published: January 2024

Abstract

Background
Drug resistance is an important constraint on clinical outcomes in advanced cancers. LAMP2A is a limiting protein in molecular chaperone-mediated autophagy. This study was aimed to explore LAMP2A function in cisplatin resistance colorectal cancer (CRC) to seek new ideas for CRC clinical treatment.
Methods
In this study, LAMP2A expression was analyzed by molecular experimental techniques, such as qRT-PCR and western blot. Then, LAMP2A in cells was interfered by cell transfection experiments. Subsequently, the function of LAMP2A on proliferation, migration, invasion, DDP sensitivity, and autophagy of CRC/DDP cells were further investigated by a series of experiments, such as CCK-8, transwell, and western blot.
Results
We revealed that LAMP2A was clearly augmented in DDP-resistant CRC and was releated to poor patient prognosis. Functionally, LAMP2A insertion remarkably CRC/DDP proliferation, migration, invasion ability and DDP resistance by strengthen autophagy. In contrast, LAMP2A knockdown limited the proliferation, migration, and invasion while heightened cellular sensitivity to DDP by restraining autophagy in CRC/DDP cells. Furthermore, LAMP2A silencing was able to curb tumor formation and enhance sensitivity to DDP in vivo.
Conclusion
In summary, LAMP2A boosted malignant progression and DDP resistance in CRC/DDP cells through mediating autophagy. Clarifying LAMP2A function in DDP resistance is promising to seek cancer therapies biomarkers targeting LAMP2A activity.
OsLSC6 regulates leaf sheath color and cold tolerance in rice revealed by metabolite genome wide association study
Authors: Lv Shuwei , Tang Xuan , Jiang Liqun , Zhang Jing , Sun Bingrui , Liu Qing , Mao Xingxue , Yu Hang , Chen Pingli , Chen Wenfeng , Fan Zhilan , Li Chen 
Published: January 2024

Abstract
Plant metabolites including anthocyanins played an important role in the growth of plants, as well as in regulating biotic and abiotic stress responses to the environment. Here we report comprehensive profiling of 3315 metabolites and a further metabolic-based genome-wide association study (mGWAS) based on 292,485 SNPs obtained from 311 rice accessions, including 160 wild and 151 cultivars. We identified hundreds of common variants affecting a large number of secondary metabolites with large effects at high throughput. Finally, we functionally identified a novel gene named OsLSC6 (Oryza sativa leaf sheath color 6) which encoded a UDP 3-O-glucosyltransferase, and involved in the anthocyanin biosynthesis of Cyanidin-3-Galc (sd1825) responsible for leaf sheath color, and results in significant different accumulation of sd1825 between wild (purple) and cultivars (green). The knockout transgenic experiments results showed that OsLSC6 regulated the biosynthesis and accumulation of sd1825, controlled the purple leaf sheath. Our further research revealed that OsLSC6 also confers resistance to cold stress during the seedling stage in rice. And we identify that a SNP in OsLSC6 is responsible for the leaf sheath color and chilling tolerance, supporting the importance of OsLSC6 in plant adaption. Our study demonstrates that metabolites-to-genes analysis by mGWAS could provide a powerful complementary tool for functional gene identification and OsLSC6 is a vital regulator during anthocyanin biosynthesis and abiotic stress responses, which promising candidate in future rice breeding and improvement.
The correlation of ferroptosis and DNA repair in individuals with colorectal cancer
Authors: Jin Meiying , Yang Jingqing , Li Lei , Li Wei , Jiang Xia , Zhang Peng 
Published: January 2024

Abstract
Crosstalk between ferroptosis and DNA repair is shown in various human illnesses, including malignancies. This study aims to develop a prognostic signature, using ferroptosis and DNA repair-related gene (DRFG), to forecast the prognosis and therapeutic responsiveness of colorectal cancer (CRC) patients. 34 DRFGs related to oxidative stress and ferroptosis were identified. Bioinformatics analysis, utilizing expression profiles of these genes and clinical data, categorized CRC patients into two DRFG clusters, revealing differentially expressed genes (DEGs) and associations with patient survival and immune cell infiltration. Risk scores based on prognosis-linked genes in both clusters were used to construct prognostic signatures. Low-risk patients demonstrated better outcomes, increased immune cell infiltration, and improved responses to chemotherapy and immune checkpoint blockade compared to high-risk patients. These results were successfully validated across multiple independent datasets, suggesting that low-risk CRC could be considered a hot tumor, while high-risk CRC is a cold tumor. The study also verified the expression levels of 6 characteristic genes in CRC and adjacent normal tissues to identify potential biomarkers. In conclusion, the study identified 34 DRFGs and developed a prognostic signature, demonstrating its efficacy in predicting survival and treatment response in CRC patients. These results hold promise for guiding precise treatment strategies in clinical practice and distinguishing between cold and hot tumors in CRC.
Dimethylmercury as a Source of Monomethylmercury in a Highly Productive Upwelling System
Authors: Adams Hannah M. , Cui Xinyun , Lamborg Carl H. , Schartup Amina T. 
Published: January 2024

Abstract

Monomethylmercury (MMHg) is a neurotoxicant that biomagnifies in marine food webs, reaching high concentrations in apex predators. To predict changes in oceanic MMHg concentrations, it is important to quantify its sources and sinks. Here, we study mercury speciation in the California Current System through cruise sampling and modeling. Previous work in the California Current System has found that upwelling impacts mercury biogeochemistry by transporting mercury-enriched deep waters to productive surface waters. These upwelled waters originate within the California Undercurrent water mass and are subsequently advected as a surface water parcel to the California Current. By comparing the two major water masses, we find that the California Undercurrent contains elevated total Hg and DMHg concentrations by 57% and 60%, respectively, compared to the California Current. We explain that these differences result from losses during advection, specifically scavenging and DMHg demethylation. We calculate a net DMHg demethylation rate constant of 1.8 ± 0.9 % d-1 and build an empirically constrained mass budget model to demonstrate that DMHg demethylation accounts for 59% of surface MMHg sources. These findings illustrate that DMHg is a significant source of MMHg in this region, challenging the current understanding of the major sources of marine MMHg.
Automatic Fire Suppression of Home Automation System Using Graphic System Design
Authors: Marimuthu  , Janani  , R Kaviyanjali , N Priyanka , M Suba 
Published: January 2024

Abstract
This project involves the development of Fire Suppression System (FSS) leveraging of Home automation system using Graphical System design (LabVIEW) software and Arduino microcontroller for real-time monitoring and control. The system integrates an Infrared (IR) flame sensor, an MQ2 gas sensor to detect and suppress fire hazards efficiently. The LabVIEW interface facilitates real-time monitoring of sensor data, allowing users to visualize and analyze the environmental conditions. Through this system, fires are automatically subdued, enhancing safety without requiring human intervention. This advances fire safety by swiftly containing threats and minimizing potential damage.
Evaluating ChatGPT’s Accuracy in Providing Screening Mammography Recommendations among Older Women: Artificial Intelligence and Cancer Communication
Authors: Braithwaite Dejana , Karanth Shama D. , Divaker Joel , Schoenborn Nancy , Lin Kenneth , Richman Ilana , Hochhegger Bruno , O'Neill Suzanne , Schonberg Mara 
Published: January 2024

Abstract

Abstract
Objective: The U.S. Preventive Services Task Force (USPSTF) recommends biennial screening mammography through age 74. Guidelines vary as to whether or not they recommended mammography screening to women aged 75 and older. This study aims to determine the ability of ChatGPT to provide appropriate recommendations for breast cancer screening in patients aged 75 years and older.
Methods: 12 questions and 4 clinical vignettes addressing fundamental concepts about breast cancer screening and prevention in patients aged 75 years and older were created and asked to ChatGPT three consecutive times to generate 3 sets of responses. The responses were graded by a multi-disciplinary panel of experts in the intersection of breast cancer screening and aging. The responses were graded as ‘appropriate’, ‘inappropriate’, or ‘unreliable’ based on the reviewer’s clinical judgment, content of the response, and whether the content was consistent across the three responses. Appropriateness was determined through a majority consensus.
Results: The responses generated by ChatGPT were appropriate for 11/17 questions (64%). Three questions were graded as inappropriate (18%) and 2 questions were graded as unreliable (12%). A consensus was not reached on one question (6%) and was graded as no consensus.
Conclusions: While recognizing the limitations of ChatGPT, it has potential to provide accurate health care information and could be utilized by healthcare professionals to assist in providing recommendations for breast cancer screening in patients age 75 years and older. Physician oversight will be necessary, due to the possibility of ChatGPT to provide inappropriate and unreliable responses, and the importance of accuracy in medicine.
Predecir la dislexia evolutiva antes de la instrucción lectora: una revisión sistemática
Authors: Quiroga Bernardos Cristina , López Gómez Santiago , Iglesias-Souto Patricia María , Taboada Ares Eva María 
Published: January 2024

Introducción: La dislexia evolutiva es una alteración neurobiológica con perfil heterogéneo que se diagnostica tras comprobar que un niño no ha aprendido a leer como se esperaba. Su detección, antes del inicio de la instrucción lectora, tiende a ser imprecisa. Objetivo: El objetivo de este estudio fue identificar y valorar habilidades psicolingüísticas que, evaluadas antes de la instrucción formal de la lectura, pueden predecir dificultades de lectura posteriores y guiar una intervención temprana. Método: Se llevó a cabo una revisión sistemática (años 2010-2020) siguiendo la declaración PRISMA en las bases de datos PsycINFO, Medline, Web of Science, Eric y SCOPUS. Resultados: Se seleccionaron 42 estudios que confirman que determinadas habilidades psicolingüísticas son relevantes para predecir el éxito lector. Conclusiones: Conciencia fonológica, velocidad de denominación, conocimiento del alfabeto y memoria fonológica presentan una fuerte correlación con el progreso inicial de la lectura y predicen las habilidades lectoras posteriores y la dislexia evolutiva.
Development of Evidence-based Core Information Guideline for Health Communication of Tobacco Control–the Effect of Smoking on Risks of Female Disease: A Delphi Survey
Authors: Liu Jin , MAO Huijia , HAO Yunyi , CAO Meiling , CHEN Leilei , QI Fei , ZHANG Ying , JIN Xuezheng , WU Yibo 
Published: January 2024

Objective To form the Evidence-based Core Information Guideline for
Health Communication of Tobacco Control-the Effect of Smoking on Risks
of Female Disease and reach a consensus based on Delphi method. Design A
Delphi survey Setting The International Practice Guidelines Registry
Platform (http: //www.guidelines-registry.org/.). The registration
number is IPGRP-2020CN072. Sample 32 multidisciplinary experts including
clinicians, nurses, health communication staff, methodologists,
epidemiologists, pharmacists, health economics experts, audience
representatives and government managers. Methods From May 2020 to March
2021, the preliminary studies of the guideline including collection and
grade of evidence, survey of public preference and value, etc were
completed. Then the Delphi survey involving panel of experts was
undertaken to grade the strength of recommendation integrated with
research evidence, clinical experience, patients’ value and preference
in order. Results After two rounds of Delphi survey, 47 of 51 core
information has reached a consensus (Consensus rate: 92.17%, 12 strong
recommendations and 35 weak recommendations). The positive coefficients
of experts are 100% (32/32) and 84.38% (27/32) respectively. The
coefficient of variation (CV) of each core information is less than
0.35. Conclusion Evidence-based Core Information Guideline for Health
Communication of Tobacco Control-the Effect of Smoking on Risks of
Female Disease has good authority, representativeness and reliability,
which will be of great significance to improve the effectiveness of
health communication of tobacco control and provide structured
suggestions for future guidelines research. Key words Tobacco
control；Smoking；Smoking cessation；Health communication；Female
disease; Delphi Technique
Anatomical and Functional Assessment of Upper Airway Performance Using Innovative NasoOroSpirometer and Cone Beam Computed Tomographic Diagnostic Devices in Children with Adenoid Hypertrophy -- Observational Study
Authors: Zomkowska Edyta , Grzybowska-Detka Sylwia , Zakrzewska Magdalena , Romaszko Jerzy , Barański Robert , Zając Andrzej , Zajączkiewicz Hanna , Nitkiewicz Szymon , Kukwa Andrzej 
Published: January 2024

Introduction. Adenoid hypertrophy leading to upper airway blockage is
the most common cause of sleep apnoea syndrome in children. Moreover,
the following disturbances can occur: normal speech resonance
impairment, middle ear ventilation difficulties, and the development of
an abnormal oral breathing pattern. Abnormal craniofacial growth and
occlusal abnormalities can be observed as well. Objectives.The aim of
the study was to confirm the relationship between respiratory disorders
with the impaired upper airway patency resulting from reduced
nasopharyngeal space. Moreover, an attempt was made to validate a new
medical device, a NasoOroSpirometer, for diagnosing respiratory
disorders resulting from adenoid hypertrophy. Design and Setting The
NasoOroSpirometer is made up of three anemometric sensors (two nasal and
one additional oral sensor) and is used to measure the airflow through
the upper airways (UA). A measurement of the flow of air simultaneously
through both nasal orifices was carried out in 105 children aged 4-8
years. The values obtained included the number of inhalations per
minute, the inspiratory time and the inhalation volume. Results and
discussion. The study demonstrated that children with adenoid
hypertrophy had a statistically significantly lower number of
inhalations through the nose than children from the control group. The
current results demonstrated no statistically significant difference
between the volume and the number of inhalations in both a combined
analysis and in one analysis conducted separately for each nasal
passage. The demonstrated statistically significant difference is most
probably due to the oral compensation for the inefficient nasal
respiratory pattern. A NasoOroSpirometric examination can be a screening
tool in the assessment of UA patency disorders and an indicator for the
eligibility for instrumental or imaging examinations.
Sweet Syndrome following SARS-CoV-2 CoronaVac vaccine
Authors: Nesrine Ben Salah , Korbi Mouna , Fadhel Najeh Ben , Safa Idoudi , Chahed Ferdaous , Njima Manel , Belhadjali Hichem , Amri Montassar , Aouam Karim , Zili Jameleddine 
Published: January 2024

We reported the 5 published case of COVID-19
vaccine inducing SS and the first case to be induced by an inactivated
SARS-CoV-2 vaccine « CoronaVac ». The short latency time strongly
supports the pathogenic role of the vaccine as a cause of SS in our
case.