bluuebunny/crossref_metadata_2025_split huggingface dataset:
Processivity and BDNF-dependent modulation of signalling endosome axonal transport are impaired in aged mice
Authors: Villarroel-Campos David , Rhymes Elena R. , Tosolini Andrew P. , Malik Bilal , Vagnoni Alessio , Schiavo Giampietro , Sleigh James N. 
Published: January 2025

AbstractA healthy nervous system is reliant upon an efficient transport network to deliver essential cargoes throughout the extensive and polarised architecture of neurons. The trafficking of cargoes, such as organelles and proteins, is particularly challenging within the long projections of neurons, which, in the case of axons, can be more than four orders of magnitude longer than cell bodies. It is therefore unsurprising that disruptions in axonal transport have been reported across neurological diseases. A decline in this essential process has also been identified in many aging models, perhaps compounding age-related neurodegeneration. Via intravital imaging, we recently determined that, despite a reduction in overall motility, the run speed and displacement of anterograde mitochondrial transport were unexpectedly enhanced in aged mouse peripheral nerves. Here, to determine how aging impacts a different axonal cargo, we evaluatedin vivotrafficking of signalling endosomes in motor axons of mouse sciatic nerves from 3 to 22 months. Contrasting with mitochondria, we did not detect alterations in signalling endosome speed, but found a consistent rise in pausing that manifested after 18 months. We then treated muscles with brain-derived neurotrophic factor (BDNF), which regulates axonal transport of signalling endosomes in motor neurons; however, we observed no change in the processivity defect at 22 months, consistent with downregulation of the BDNF receptor TrkB at the neuromuscular junction. Together, these findings indicate that aging negatively impacts signalling endosome trafficking in motor axons likely through dampened BDNF signalling at the motor neuron-muscle interface.Graphical Abstract
Immunity gene silencing increases transient protein expression inNicotiana benthamiana
Authors: Dodds Isobel L. , Watts Emma C. , Schuster Mariana , Buscaill Pierre , Tumtas Yasin , Holton Nicholas J. , Song Shijian , Stuttmann Johannes , Joosten Matthieu H. A. J. , Bozkurt Tolga O. , van der Hoorn Renier A. L. 
Published: January 2025

ABSTRACTAgroinfiltration ofNicotiana benthamianais routinely used for transient gene expression in plant sciences and molecular pharming. Here, we depleted transcripts of 21 different immunity-related genes through virus-induced gene silencing (VIGS) to identify genes that hamper transient expression in juvenile plants. These experiments uncovered that silencing ofethylene insensitive- 2(EIN2), receptor-like kinaseCERK1, transcription factor regulatornonexpressor of pathogenesis-related genes 1(NPR1) andisochorismate synthase(ICS), increases transient GFP expression by 2-, 4-, 4- and 11-fold, respectively. Accordingly, thenpr1a/npr1bdouble mutant ofN. benthamianadoes indeed facilitate increased protein accumulation when transiently expressed. These results indicate that glycan perception through CERK1, and ET and SA signaling pathways via EIN2 and NPR1/ICS, respectively, contribute to suppressed transient gene expression in frequently used juvenileN. benthamianaplants.
Novel Laser Technology Enables 10x Faster SRS Imaging and Rapid Tuning in Biological Samples
Authors: Reinkensmeier Lenny , Siegmund René , Bates Mark , Stibenz Gero , Trabs Peter , Popien Stefan , Rimke Ingo , Heuke Sandro , Egner Alexander 
Published: January 2025

AbstractStimulated Raman Scattering (SRS) microscopy was developed for the label-free detection of molecular groups, addressing the speed limitations of spontaneous Raman microscopy. Standard SRS microscopy typically operates with laser sources at an 80 MHz repetition rate and a color-tuning speed of approximately 0.1 Hz to target different molecular groups. Here, we present a novel laser system that overcomes these speed limitations, achieving an order-of-magnitude improvement in both color-tuning and imaging speed. Our system features a reduced repetition rate of 40 MHz, enabling SRS imaging that is ten times faster than standard systems while maintaining the same average power at the sample. This is achieved through increased pulse energy and laser modulation at half the repetition rate. Furthermore, the system provides nearly ten times faster color-tuning across an extended range (660–1010 nm) by employing angle-tuning of nonlinear crystals instead of temperature-tuning. The improved performance is demonstrated in direct comparison with a standard SRS laser system, showcasing the potential for significantly enhanced imaging capabilities.
Safer facility floor designing: a tribological insight into optimising ceramic tile surface textures for enhanced slip resistance
Authors: Kim In-Ju 
Published: January 2025

Purpose
This study aims to enhance pedestrian safety by investigating the slip resistance of facility floors with ceramic tile surfaces and identifying critical surface roughness parameters that influence slip resistance.

Design/methodology/approach
Dynamic friction tests and comprehensive surface roughness analyses were conducted on ceramic tiles. Statistical analysis identified optimal roughness ranges for key parameters (Ra, Rp, Rt, Rz and Rv) to ensure the dynamic friction coefficient exceeds 0.5 to meet safety requirements. This study assessed different textures under dry, damp and foamy conditions.

Findings
The findings reveal that ceramic tile surfaces with higher peak heights and deeper valleys provide superior traction, particularly in damp and foamy conditions. The results demonstrate that specific surface textures can effectively mitigate slip and fall hazards by enhancing grip and increasing surface interaction points. This study establishes optimal roughness ranges for the critical parameters, providing a robust framework for improving slip resistance.

Originality/value
This study offers practical guidelines for designing safer ceramic tiles, emphasising the importance of tailored surface roughness to improve traction. It highlights the importance of considering environmental factors in slip resistance assessments, offering valuable insights for manufacturers, designers and policymakers. By focusing on specific surface textures, this study advances the development of safer built environments in public facilities. Future research directions should explore a broader range of tile finishes, footwear types and contaminant scenarios to refine the understanding of traction performance further.
Comparative transcriptomics and metabolomics provide insight into degeneration‐related physiological mechanisms of Morchella importuna after long‐term preservation
Authors: Chen Ying , Cao Xuelian , Xie Liyuan , Tang Jie , Liu Lixu , Wang Di , Wu Xiang , Liu Tianhai , Yu Yang , Wang Yong , Martin Francis , Peng Weihong , Tan Hao 
Published: January 2025

AbstractAscomycetes fungi are often prone to degeneration. Agricultural production of the prized ascomycete mushroom Morchella importuna (black morel) typically suffers from reduced yield and malformed ascocarps owing to culture degeneration. This study compared M. importuna cultures subjected to five different long‐term preservation treatments, using transcriptomics and metabolomics. Avoiding repeated subculturing in combination with nutrient‐limited conditions was found to be the most beneficial method for maintaining the fruiting capability of morels. The expression of the gene sets involved in cysteine and methionine metabolism and nucleocytoplasmic transport was upregulated under nutrient‐limited and nutrient‐rich conditions, respectively. This increased expression was accompanied by differential accumulation of metabolites involved in nucleobase metabolism. Repeated subculturing triggered dissimilar changes in the functional modules under nutrient‐rich and nutrient‐limited conditions. A diverse set of cellular biochemical processes related to carbon metabolism were altered by repeated subculturing under nutrient‐rich conditions, whereas glycerophospholipid and purine metabolism were key functions affected by repeated subculturing under nutrient‐limited conditions. Altogether, metabolic alterations related to sulfur‐containing amino‐acid biosynthesis, DNA repair, and cellular structural maintenance contributed to improved preservation outcomes in terms of morel fruiting capability. Our findings contribute to a more detailed understanding of the molecular mechanisms related to subculturing and fruiting of ascomycete macrofungi after long‐term preservation.
Uruguayan Grasslands: A Threatened Natural Asset
Authors: Guido Anaclara , López‐Mársico Luis , Rodríguez Claudia , Gallego Federico , Lezama Felipe , Baeza Santiago , Pañella Pedro , Ríos Cecilia , Costa Beatriz , Pezzani Fabiana , García Silvina , Piñeiro Gervasio , Fernández Gastón , Tommasino Andrea , Mello Ana Laura , Altesor Alice , Paruelo José 
Published: January 2025

ABSTRACTGrasslands are often mis‐defined, mainly due to the legacy of colonial narratives in several regions, which have described them as deforested, unproductive or wastelands. They have been largely ignored in global conservation and protection policies, leading to increased exploitation. Although there is much accumulated evidence on the ecosystem services that grasslands provide, in Uruguay, this ecosystem is still neglected in parts of society and for most sustainability policies. Twenty percent of Uruguayan grasslands have been replaced by other land uses (crops and forestry) in recent decades. Nevertheless, large areas of natural grasslands still remain and their preservation is crucial. The aim of this work was to show the value of Uruguayan grasslands through a brief review of their natural history and their multiple natural assets (i.e., biodiversity, ecosystem services, cultural identity and economic activities). We also outline some of the advances in public policy initiatives, but emphasise that such policies are critically underdeveloped in terms of conservation, threatening the existence of grasslands in Uruguay for future generations.
Glycopyrronium 320 μg/mL in children and adolescents with severe sialorrhoea and neurodisabilities: An open‐label study extension of the SALIVA trial
Authors: Fayoux Pierre , Dinomais Mickael , Shaw Helen , Villain Frédéric , Schwartz Déborah , Gautheron Vincent , Letellier Guy , Auvin Stéphane 
Published: January 2025

AbstractAimsTo test the long‐term efficacy, safety, and impact on quality of life (QoL) of an oral paediatric formulation of 320 μg/mL glycopyrronium in the 36‐week SALIVA (Sialanar plus orAl rehabiLitation against placebo plus oral rehabilitation for chIldren and adolescents with seVere sialorrhoeA and neurodisabilities) trial.MethodIn the initial 12‐week blinded period, 87 children with neurodisabilities and severe sialorrhoea were randomized to 320 μg/mL glycopyrronium versus placebo. In the subsequent 24‐week open‐label study extension, 74 children received 320 μg/mL glycopyrronium (37 continued glycopyrronium, 37 switched from placebo).ResultsThe open‐label study extension population included 39 males and 35 females. The median age was 10 years 2 months (quartile 1, quartile 3: 7 years 5 months, 14 years 7 months; range: 3 years 5 months–17 years 8 months). Over 36 weeks, continued 320 μg/mL glycopyrronium resulted in a median 39‐point reduction in Drooling Impact Scale (DIS) score from baseline (quartile 1, quartile 3: −51, −21; p < 0.001), with an 81.1% response rate (DIS improvement ≥ 13.6 points) and a 70.3% good response rate (≥ 28 points). Improvements in the impact of drooling on QoL seen in the blinded period were sustained with continued glycopyrronium. Treatment‐related adverse events occurred most frequently during titration (0–4 weeks: 40.9%; 5–20 weeks: 32.4% in those who switched). Constipation was the most common adverse event.InterpretationLong‐term treatment with 320 μg/mL glycopyrronium resulted in significant sustained improvements in drooling and QoL, with fewer adverse events after initial titration and overall good tolerability.
Enhanced Electricity Forecasting for Smart Buildings Using a TCN‐Bi‐LSTM Deep Learning Model
Authors: Gautam Sandeep Kumar , Shrivastava Vinayak , Udmale Sandeep S. 
Published: January 2025

ABSTRACTIntegration of sensor technology and advanced software empowers consumers to manage energy usage proactively. This proactive approach yields positive impacts at both micro and macro levels, benefiting individuals and contributing to broader environmental conservation efforts. By leveraging predictive models, consumers can make informed decisions that serve their interests and promote a greener and more sustainable future for all. Thus, energy consumption (EC) prediction is crucial for effective resource management. In this study, we propose an innovative deep‐learning approach to predict EC, focusing specifically on smart buildings. Our model utilises a hybrid deep learning architecture to effectively capture low and high information patterns present in multivariate time series data of various sensors deployed in smart buildings and numerous influencing factors. To address the nonlinear and dynamic nature of this data, our model combines a deep neural network (DNN) with a deep learning sequential model (DLS). Specifically, temporal convolutional networks (TCN) within the DNN family are employed to extract various trends from the data, while the DLS model, which consists of Bi‐directional Long Short‐term Memory Networks (Bi‐LSTM), is employed to learn and capture these trends effectively. Consequently, we present a hybrid deep learning framework that leverages for learning multivariate time series data related to EC with shared feature representation. To validate our approach, we extensively evaluate our model using a dataset from an office building in Berkeley, California. Experimental results demonstrate that our model achieves satisfactory accuracy in EC prediction. For the 7‐h horizon and on multivariate TS data, an R2 of 0.97 is realised for the proposed model. This is confirmed by the 1.65% improvement in transiting from univariate to multivariate data, which supports using multiple modalities.
Actually Existing Geopolitics of Urban Knowledge Production. Questioning the ‘From Anywhere’ of Urban Theorising
Authors: Governa Francesca , Iacovone Chiara 
Published: January 2025

ABSTRACTThis article addresses the geopolitics of urban knowledge production by focussing on articles published in the past six years (2018–2023) in six ‘international’ journals. It aims to contribute to ongoing debates on the questioning of Anglo‐American hegemony and the decolonisation of geographic scholarship through the analysis of the where of urban knowledge production in terms of the location of the authors and the places studied. Our analyses clearly highlight the highly selective nature of the geopolitics of urban knowledge production. And yet, this selectivity is not only based on, and related to, the North‐South divide, but also develops between cities and universities, defining an unequal and multiscalar geography of who and from where one can speak and be heard. Moreover, the geography of the places studied contradicts any claims of post/decolonising urban theory, of ‘urban theorising from anywhere’, of Southern, subaltern, alternative urban perspectives, and so on. As it emerges in ‘international’ publishing, the current situation of urban knowledge production clashes with the calls for a global urban knowledge and asks for a common effort to go beyond it.
An Occult Primary Thymic Mucosa‐Associated Lymphoid Tissue Lymphoma With Sjögren's Syndrome Revealed by CXCR4 Targeted 68Ga‐Pentixafor PET/CT
Authors: Gao Xuehan , Peng Xingtong , Wang Rongxi , Qian Zhihong , Zhou Xiaoyun , Zhu Zhaohui , Chen Yeye 
Published: January 2025

ABSTRACTMucosa‐associated lymphoid tissue (MALT) lymphoma is an extranodal low‐grade non‐Hodgkin lymphoma that extremely rarely localises to the mediastinum. A 34‐year‐old female with chronic arthralgia, sicca and rash was found to have a well‐demarcated mediastinal cystic mass with equivocal nodular enhancement within the cystic wall on chest CT during a workup for Sjögren's syndrome. Subsequent 68Ga‐Pentixafor‐PET/CT revealed focal uptake increase within the cystic capsule. The patient underwent thoracoscopic resection of the mediastinal lesion, and pathology revealed MALT lymphoma in the wall of a thymic cyst. This case highlights that 68Ga‐pentixafor PET/CT could be valuable for the non‐invasive detection of occult thymic MALT lymphoma.Trial Registration: ClinicalTrials.gov. (www.clinicaltrials.gov, NCT06086327)
Unveiling Molecular Dynamics of MeCp2, CDKL5 and BDNF in the Hippocampus of Individuals With Intractable Mesial Temporal Lobe Epilepsy
Authors: Meshkinkhood NoorMohammad , Barati Dowom Parastoo , Noorbakhsh Farshid , Ghadipasha Masoud , Gharehdaghi Jaber , Kellinghaus Christoph , Speckmann Erwin‐Joseph , Khaleghi Ghadiri Maryam , Stummer Walter , Gorji Ali 
Published: January 2025

ABSTRACTMutations occurring in the MeCp2, CDKL5 and BDNF genes have been linked to epileptogenesis in various epilepsy syndromes. This study employed bioinformatics analysis of transcriptomic data to examine the interrelationship among these genes in both epileptic and healthy individuals. Moreover, we assessed the expression of MeCp2, CDKL5 and BDNF at both mRNA and protein levels in human hippocampal tissues obtained from 22 patients undergoing epilepsy surgery for mesial temporal lobe epilepsy (MTLE) as well as from 25 autopsied specimens. Bioinformatics findings suggest that MeCp2, CDKL5 and BDNF genes play a role in regulating genes associated with epilepsy and disruptions in these genes may contribute to epilepsy development. Furthermore, the study reveals significantly lower MeCp2 and CDKL5 protein levels in the epileptic hippocampus compared to controls. Positive correlations are observed between MeCp2 and CDKL5 mRNA expression in autopsied samples and between CDKL5 and BDNF mRNA expression in epileptic hippocampal tissues. Differences in mRNA expression correlation patterns of MeCp2 and CDKL5 with BDNF are found between epileptic and control hippocampal tissues. Moreover, a significant positive correlation between MeCp2 and CDKL5 protein expression is noted in control hippocampal tissues. Our data suggest that altered expression of MeCp2, CDKL5 and BDNF within the hippocampus may contribute to epileptogenic processes in MTLE, impacting seizure characteristics, surgical outcomes and responses to antiepileptic drugs. Alterations in the expression of MeCp2, CDKL5 and BDNF within the hippocampus might contribute to the epileptogenic processes in MTLE. These changes could be linked to distinct functional consequences in epilepsy.
Regulatory Fragmentation
Authors: KALMENOVITZ JOSEPH , LOWRY MICHELLE , VOLKOVA EKATERINA 
Published: January 2025

ABSTRACTRegulatory fragmentation occurs when multiple federal agencies oversee a single issue. Using the full text of the Federal Register, the government's official daily publication, we provide the first systematic evidence on the extent and costs of regulatory fragmentation. Fragmentation increases the firm's costs while lowering its productivity, profitability, and growth. Moreover, it deters entry into an industry and increases the propensity of small firms to exit. These effects arise from redundancy and, more prominently, from inconsistencies between government agencies. Our results uncover a new source of regulatory burden, and we show that agency costs among regulators contribute to this burden.
Piloting Multiple Micronutrient Supplementation Within the Routine Antenatal Care System in Ethiopia: Insights From Stakeholders
Authors: Berhanu Anene Tesfa , Defar Atkure , Taye Girum , Daba Alemneh Kabeta , Alemayehu Senait , Tessema Bedasa , Zenebe Kalkidan , Opondo Charles , Tollera Getachew , Hailu Mesay , Schellenberg Joanna , Persson Lars Ake , Marchant Tanya , Tessema Masresha 
Published: January 2025

ABSTRACTIn low and middle‐income countries, addressing maternal and child nutrition needs is crucial. Prenatal multiple micronutrient supplementation (MMS) holds promise in reducing low birthweight and preterm births. Ethiopia is considering a transition from the provision of iron‐folate supplementation to the provision of MMS in antenatal care, guided by WHO guidelines. This paper explores stakeholders' early perceptions and experiences in piloting that transition in five Ethiopian regions, informing decision making about future policy and scale‐up. We used a qualitative study involving interviews and document reviews to assess the MMS pilot, analyzing themes with Nvivo 12 software. Key informant interviews revealed challenges such as supply chain issues and funding constraints, along with proposed improvement strategies such as closer coordination within existing structures. The importance of engaging with communities to create demand was emphasized, as was the need for capacity‐building efforts utilizing existing staff in the health system with a clear plan for ongoing refresher training. Finally, enhancing the monitoring and evaluation framework, including a dedicated regional team for supportive supervision, was vital for accountability. In conclusion, prioritizing supply chain strengthening, community engagement through demand creation, coordination, and continuous capacity building for healthcare workers were thought to be crucial for the MMS program's success.Trial RegistrationClinicalTrials.gov Identifier: NCT05708183 Registered 01 February 2023—Retrospectively registered, https://classic.clinicaltrials.gov/ct2/show/NCT05708183.
Cutaneous Manifestations of NAXD or NAXE Deficiency: A Literature Review for the Dermatologist
Authors: Abdulkarim Boraan , Mittal Setu , Vahidnezhad Hassan , Davis Dawn Marie , Camilleri Michael J. , Mohandesi Nessa Aghazadeh 
Published: January 2025

ABSTRACTNicotinamide adenine dinucleotide phosphate hydrate dehydratase (NAXD) and nicotinamide adenine dinucleotide phosphate hydrate epimerase (NAXE) deficiencies are rare autosomal recessive metabolic disorders characterized by severe neurological manifestations during infancy. In affected individuals, febrile illnesses can trigger progressive encephalopathy often accompanied by distinctive skin eruptions, resulting in high mortality rates. This study reviews the literature on NAXD/NAXE deficiencies, focusing on dermatological manifestations and their correlation with genotypic variations and treatment responses. A comprehensive literature search identified 45 patients with 31 pathogenic/likely pathogenic mutations, and a median age of onset at 1.16 years. Patients with NAXE deficiency exhibited a broader range of age of symptom onset compared to those affected with NAXD deficiency. Fever or infections were identified as the most common triggers for decompensation episodes. Skin manifestations were observed in 31% of patients with whole cell NAXD and NAXE deficiencies. The characteristic skin eruption comprises well‐demarcated erythematous and erosive plaques progressing to blistering and necrosis, predominantly affecting flexural surfaces. The mortality rate was 78%, with survivors experiencing varying degrees of neurological sequelae. Niacin/nicotinamide supplementation resulted in improvements in skin lesions and survival rates. The review underscores the critical role of dermatologists in early diagnosis and intervention of NAXE and NAXD deficiencies. Recognizing characteristic skin manifestations is essential, particularly in patients with acute neurological decline following infections or vaccinations. Early intervention with specific supplements shows promise; however, further research is needed to establish standardized treatment protocols and enhance patient outcomes.
Discovery of the Novel HLA‐C*01:282 Allele by Next‐Generation Sequencing
Authors: Zhao He , Webster Destinie , Parsyak Svetlana , Hassan Abubaker , Mostafa Ahmed 
Published: January 2025

ABSTRACTThe novel allele HLA‐C*01:282 has a single non‐synonymous mutation compared to HLA‐ C*01:02:01:01 in codon 100 in Exon 3.