bluuebunny/crossref_metadata_2025_split huggingface dataset:
Graphene‑Assisted Assembly of Electrically and Magnetically Conductive Ceramic Nanofibrous Aerogels Enable Multifunctionality
Authors: Li Bin , Tian Haoyuan , Li Lei , Liu Wei , Liu Jiurong , Zeng Zhihui , Wu Na 
Published: February 2024

AbstractCeramic aerogels are gaining increasing attention due to their low density, high‐temperature resistance, and excellent chemical stability. However, conventional ceramic aerogels are hindered by their intrinsic brittleness and limited dielectric properties, which restrict their scalable manufacturing and multifunctional applications. Here, ultralight, biomimetic porous, electrically and magnetically conductive ceramic nanofibrous aerogels composed of silicon dioxide (SiO2) nanofibers, graphene, and metal–organic framework (MOF) derivatives are prepared through an ice‐templating freeze‐casting followed by annealing approach. The renewable SiO2 nanofibers form robust bonding points with graphene, constructing interconnected high‐porosity aerogels with good mechanical resilience. This allows for efficient integration of MOF‐derived magnetic nanoparticles associated with a synergistic mechanical enhancement. The synergies of the dielectric and magnetic components, combined with the uniformly arranged sheet‐like cell walls which facilitate the outstanding electromagnetic wave absorption performance. Moreover, the hydrophobic ceramic aerogels showcase excellent magnetothermal conversion, contributing to the application in wireless therapy, antibacterial, and magnetothermal deicing. Furthermore, the nanofibrous aerogels exhibit good thermal stability and insulation properties, rendering them highly suitable for thermal management devices in extreme conditions. With the renewable, convenient, and scalable manufacturing method, these multifunctional ceramic nanofibrous aerogels thus hold great promise in electromagnetic protection, wireless heating, and next‐generation thermal management devices.
Drug‐Loaded Bacillus Calmette–Guérin Bacteria for Immuno‐Chemo Combo Therapy in Bladder Cancer
Authors: Liu Kangkang , Wang Lining , Peng Jing , Lyu Yuanji , Li Yiming , Duan Dengyi , Zhang Wenyi , Wei Guojiang , Li Taipeng , Niu Yuanjie , Zhao Yang 
Published: February 2024

AbstractIntravesical Bacillus Calmette–Guérin (BCG) is a well‐established strategy for managing high‐risk nonmuscle‐invasive bladder cancer (NMIBC); however, over half of patients still experience disease recurrence or progression. Although the combined intravesical instillation of various chemotherapeutic drugs is implemented in clinical trials to enhance the BCG therapy, the outcome is far from satisfying due to severe irritative effects and treatment intolerance at high doses. Therefore, it is adopted the “biotin–streptavidin strategy” to doxorubicin (DOX)‐encapsulated nanoparticles within live BCG bacteria (DOX@BCG) to improve treatment outcomes. Adherence of BCG to the bladder epithelium helps precisely target DOX@BCG to the local tumor cells and simultaneously increases intratumoral transport of therapeutic drugs. DOX@BCG effectively inhibits cancer progression and prolongs the survival of rats/mice with orthotopic bladder cancer owing to synergism between BCG‐immunotherapy, DOX‐chemotherapy, and DOX‐induced immunogenic tumor cell death; furthermore, it exhibits improved tolerance and biosafety, and establishes antitumor immunity in the tumor microenvironment. Therefore, the drug‐loaded live BCG bacterial delivery system holds considerable potential for clinical translation in the intravesical treatment of bladder cancer.
Recent Progress in Wearable Self‐Powered Biomechanical Sensors: Mechanisms and Applications
Authors: Zhang Shaotong , Lin Xiang , Wan Ji , Xu Chen , Han Mengdi 
Published: February 2024

AbstractBiomechanical signals, such as strain variations of the skin, vibrations of the chest and throat, as well as motions of the limbs, hold immense significance in healthcare monitoring, disease diagnosis, and human‐machine interface. Examples span from monitoring blood pressure and pulse waves for atherosclerosis diagnosis, to distinguishing between metatarsalgia patients and healthy individuals by tracking their walking postures, and to voiceprint recognition and hearing aid technology based on vibration sensing. Wearable biomechanical sensors play a crucial role in providing valuable insights into one's health condition and physiological features. However, the development of high‐performance sensors capable of prolonged monitoring poses challenges. Traditional batteries have limited lifespan and pose difficulty in replacement. Using self‐powered devices for the measurement of biomechanical signals represents an attractive solution to tackle the issues caused by batteries. This review focuses on the mechanisms of wearable self‐powered biomechanical sensors, and delves into recent advancements in their applications, covering areas of cardiovascular system monitoring, acoustic signals detection, human motion tracking, and many others associated with biomechanics. A concluding section outlines the potential future prospects in this evolving field of materials and biomedical research.
First‐trimester noninvasive prenatal diagnosis of seven facioscapulohumeral muscular dystrophy type 1 families using SNP‐based amplicon sequencing: An earlier, rapid and safer way
Authors: Fu Xinyu , Zhao Zhenhua , Kong Lingrong , Li Shaojun , Li Feifei , Han Xiujuan , Sun Luming , Wu Di , Wang Yanan , Kong Xiangdong 
Published: February 2024

AbstractThe study is to explore the feasibility and value of SNP‐based noninvasive prenatal diagnosis (NIPD) for facioscapulohumeral muscular dystrophy type 1 (FSHD1) in early pregnancy weeks. We prospectively collected seven FSHD1 families, with an average gestational age of 8+6. Among these seven couples, there were three affected FSHD1 mothers and four affected fathers. A multiplex‐PCR panel comprising 402 amplicons was designed to selective enrich for highly heterozygous SNPs upstream of the DUX4 gene. Risk haplotype was constructed based on familial linkage analysis. Fetal genotypes were accurately inferred through relative haplotype dosage analysis using Bayes Factor. All tests were successfully completed in a single attempt, and no recombination events were detected. NIPD results were provided within a week, which is 4 weeks earlier than karyomapping and 7 weeks earlier than Bionano single‐molecule optical mapping (BOM). Ultimately, five FSHD1 fetuses and two normal fetuses were successfully identified, with a 100% concordance rate with karyomapping and BOM. Therefore, SNP‐based NIPD for FSHD1 was demonstrated to be feasible and accurate in early weeks of gestation, although the risk of recombination events cannot be completely eliminated. In the future, testing of more cases is still necessary to fully determine the clinical utility.
Sex and ancestry patterning of residual correlations in human dental development: Cooperative genetic interaction and phenotypic plasticity
Authors: Sgheiza Valerie 
Published: February 2024

AbstractObjectivesMost research in human dental age estimation has focused on point estimates of age, and most research on dental development theories has focused on morphology or eruption. Correlations between developing teeth using ordinal staging have received less attention. The effect of demographic variables on these correlations is unknown. I tested the effect of reference sample demographic variables on the residual correlation matrix using the lens of cooperative genetic interaction (CGI).Materials and MethodsThe sample consisted of Moorrees et al., Journal of Dental Research, 1963, 42, 1490–1502, scores of left mandibular permanent teeth from panoramic radiographs of 880 London children 3–22.99 years of age stratified by year of age, sex, and Bangladeshi or European ancestry. A multivariate cumulative probit model was fit to each sex/ancestry group (n = 220), each sex or ancestry (n = 440), and all individuals (n = 880). Residual correlation matrices from nine reference sample configurations were compared using Bartlett's tests of between‐sample difference matrices against the identity matrix, hierarchical cluster analysis, and dendrogram cophenetic correlations.ResultsBartlett's test results were inconclusive. Cluster analysis showed clustering by tooth class, position within class, and developmental timing. Clustering patterns and dendrogram correlations showed similarity by sex but not ancestry.DiscussionExpectations of CGI were supported for developmental staging. This supports using CGI as a model for explaining patterns of variation within the dentition. Sex was found to produce consistent patterns of dental correlations, whereas ancestry did not. Clustering by timing of development supports phenotypic plasticity in the dentition and suggests shared environment over genetic ancestry to explain population differences.
An Enzyme‐Cleavable Solubilizing‐Tag Facilitates the Chemical Synthesis of Mirror‐Image Proteins
Authors: Zheng Yupeng , Zhang Baochang , Shi Wei‐Wei , Deng Xiangyu , Wang Tong‐Yue , Han Dongyang , Ren Yuxiang , Yang Ziyi , Zhou Yong‐Kang , Kuang Jian , Wang Zhi‐Wen , Tang Shan , Zheng Ji‐Shen 
Published: February 2024

AbstractMirror‐image proteins (D‐proteins) are useful in biomedical research for purposes such as mirror‐image screening for D‐peptide drug discovery, but the chemical synthesis of many D‐proteins is often low yielding due to the poor solubility or aggregation of their constituent peptide segments. Here, we report a Lys‐C protease‐cleavable solubilizing tag and its use to synthesize difficult‐to‐obtain D‐proteins. Our tag is easily installed onto multiple amino acids such as DLys, DSer, DThr, and/or the N‐terminal amino acid of hydrophobic D‐peptides, is impervious to various reaction conditions, such as peptide synthesis, ligation, desulfurization, and transition metal‐mediated deprotection, and yet can be completely removed by Lys‐C protease under denaturing conditions to give the desired D‐protein. The efficacy and practicality of the new method were exemplified in the synthesis of two challenging D‐proteins: D‐enantiomers of programmed cell death protein 1 IgV domain and SARS‐CoV‐2 envelope protein, in high yield. This work demonstrates that the enzymatic cleavage of solubilizing tags under denaturing conditions is feasible, thus paving the way for the production of more D‐proteins.
Nutrient and bioactive compounds from Neltuma spp. seeds
Authors: Cittadini M. Cecilia , Bodoira Romina , Barrionuevo Damián , Labuckas Diana , Martínez Marcela , Maestri Damián 
Published: February 2024

AbstractIn the search for compounds with nutritional and functional value, seeds from several Neltuma species were examined for lipid, protein and phenolic components. Lipid contents ranged between 9.3 and 12.5 g/100 g (seed dry weight), and showed both oleic and linoleic acids predominating largely. Lipids were also a good source of tocopherols (170–603 mg total tocopherols/kg). Proteins accounted for 14.7–35.5 g/100 g seed. Essential amino acids (EAA) comprised 26.1%–40.2% of the total AA content. Based on the EAA score, proteins from all the species analyzed were deficient in leucine, isoleucine and valine, but could meet requirements for histidine, threonine, phenylalanine, tyrosine, and lysine. The qualitative patterns of phenolic compounds were similar each other (predominance of flavonoids, particularly apigenin derivatives). Keeping in mind the examined chemical components, Neltuma seeds have potential value as a source of healthy macronutrients and bioactive compounds.
Application of a One Welfare‐Based Ecosystem Model to assess wild collection for public aquariums
Authors: Fischer Brittany , Pempek Jessica A. , Flint Jaylene , Wittum Thomas , Flint Mark 
Published: February 2024

Abstract1. Public aquariums are organizations that offer their visitors an opportunity to engage with, gain knowledge of, and contribute to the conservation of species and ecosystems. Unfortunately, the ways many aquariums maintain their populations include primarily sourcing marine species from the wild. This creates the potential for negative impacts to stressed aquatic environments and a need for a holistic approach to quantify the impacts of aquarium population management. 2. The objective of this study was to implement a developed One Welfare‐Based Ecosystem Model to predict impacts of aquarium population management on humans, animals, and the environment. Ecosystem indicators were selected and grouped into physical–chemical, biological, and socio‐economic categories. Data included measurements of environmental and animal indicators at field sites frequently visited by aquariums to collect animals from, aquarium visitor surveys, and publicly available data. Model development included comparing field data to reference information and then adding weightings based on indicator significance and alpha‐testing of the model. 3. At model completion, each institution was allocated a One Welfare status. Confidence ratings of measured indicators and reference data occurred for each field site to determine the appropriateness of use of the model for each aquarium. 4. Findings included a positive One Welfare status, with collaborating institutions scoring in the good and excellent categories (81.92/100 and 92.51/100). Confidence ratings showed positive results (62/100 and 78/100) for model applicability for the field sites studied as well as future use by other institutions assessing similar parameters. 5. To maintain their recognition as leaders in conservation, public aquariums must objectively assess their direct and indirect influence on marine ecosystems moving forward.
Pro‐fluorescent probe with morpholine moiety and its reactivity towards selected biological oxidants
Authors: Modrzejewska Julia , Grzelakowska Aleksandra , Szala Marcin , Michalski Radosław , Zakłos‐Szyda Małgorzata , Podsiadły Radosław 
Published: February 2024

AbstractBiological oxidants participate in many processes in the human body. Their excessive production causes organelle damage, which may result in the accumulation of cytotoxic mediators and cell degradation and may manifest itself in various diseases. Peroxynitrite (ONOO−), hypochlorous acid (HOCl), hydrogen peroxide (H2O2), and peroxymonocarbonate (HOOCO2−) are important oxidants in biology, toxicology, and various pathologies. Derivatives of coumarin, containing an oxidant‐sensitive boronate group, have been recently developed for the fluorescent detection of inflammatory oxidants. Here, we report the synthesis and characterization of 4‐[2‐(morpholin‐4‐yl)‐2‐oxoethyl]‐2‐oxo‐2H‐chromen‐7‐yl boronic acid (MpC‐BA) as a fluorescent probe for the detection of oxidants, with better solubility in water, high stability and fast response time toward peroxynitrite and hypochlorous acid. The effectiveness of the MpC‐BA probe for the detection of peroxynitrite was measured by adding bolus ONOO− or using the co‐generating superoxide and nitrogen oxide system. MpC‐BA is oxidized by ONOO− to 7‐hydroxy‐4‐[2‐(morpholin‐4‐yl)‐2‐oxoethyl]‐2H‐chromen‐2‐one (MpC‐OH). However, peroxynitrite‐specific product (MpC‐H) is formed in the minor reaction pathway. MpC‐OH is also yielded in the reaction of MpC‐BA with HOCl, and the subsequent formation of a chlorinated MpC‐OH gives a specific product for HOCl (MpC‐OHCl). H2O2 slowly oxidizes MpC‐BA. However, the addition of NaHCO3 increased the MpC‐OH formation rate. We conclude that MpC‐BA is potentially an improved fluorescent probe detecting peroxynitrite and hypochlorite in biological settings. Complementation of the fluorescence measurements by HPLC‐based identification of chlorinated and reduced coumarin(s) will help identify the oxidants detected.
Sustainability reporting for boosting national commitment and overcoming challenges: A hierarchical model
Authors: Paridhi  , Ritika  
Published: February 2024

AbstractSustainability reporting (SR) has emerged as a critical mechanism for organizations and nations to communicate their performance in terms of environmental, social, and governance (ESG). The present study investigates the challenges that prevent SR from being useful for system transparency and accountability. It identifies formidable barriers such as intentions, knowledge gaps, resource constraints, and the intricate nature of measurement metrics which necessitating a global collaborative efforts to improve SR's adaptability and acceptance within diverse systems. In addition, this pioneering research examines the factors that influence SR adoption and commitment at the national level. In the study, total interpretive structural modelling (TISM) is used to investigate the hierarchical relationship between factors while MICMAC analysis is administered to identify driver‐dependent connections. It reveals a multi‐layered structure in which macroeconomic factors such as political stability and resource availability drive regulatory effectiveness, operational factors such as administrative efficiency and awareness synergise for progress, and performance factors provide comprehensive insights into SR maturity at national level. The findings contribute significantly to the refinement of theoretical frameworks while emphasizing the importance of SR in advancing the 2030 Agenda for Sustainable Development. It emphasizes on political will, resource allocation, and tailored regulatory frameworks for comprehensive reporting of sustainability. This study provides practical insights for managers, policymakers, and businesses navigating sustainability reporting. It identifies drivers of national SR commitment and guides corporate practices towards sustainable development goals. Understanding these issues strengthens risk management and governance, while advanced measurement tools facilitate assessment and communication. Overall, it empowers stakeholders to elevate their engagement in enhancing sustainability practices, refining governance structures, and informing policy formulation.
Engineering ORR Electrocatalysts from Co8Pt4 Carbonyl Clusters via ZIF‐8 Templating
Authors: Schneider Peter M. , Kollmannsberger Kathrin L. , Cesari Cristiana , Khare Rachit , Boniface Maxime , Roldán Cuenya Beatriz , Lunkenbein Thomas , Elsner Martin , Zacchini Stefano , Bandarenka Aliaksandr S. , Warnan Julien , Fischer Roland A. 
Published: February 2024

AbstractTo reduce the costs of proton exchange membrane fuel cells, the amount of Pt necessary to drive efficient oxygen reduction reaction (ORR) should be minimized. Particle nanostructuring, (nano‐)alloying, and metal‐doping can yield higher activities per Pt mass through tailoring catalysts owning a high number of active sites and precise electronic properties. In this work, the atom‐precise [NBnMe3]2[Co8Pt4C2(CO)24] (Co8Pt4) cluster is encapsulated and activated in a zeolitic imidazolate framework (ZIF)‐8, which unlocks the access to defined, bare Pt−Co nanoclusters, Co8±xPt4±yNC@ZIF‐8, for the fabrication of highly active ORR catalysts. Upon controlled C‐interfacing and ZIF‐8‐digestion, Co‐doped Pt NPs (Pt27Co1) with a homogenous and narrow size distribution of (1.1±0.4) nm are produced on Vulcan® carbon. Restructuring of the Pt27Co1/C catalyst throughout the ORR measurement was monitored via high‐angle annular dark field‐scanning transmission electron microscopy and X‐ray photoelectron spectroscopy. The measured ORR mass activity of (0.42±0.07) A mgPt−1 and the specific activity of (0.67±0.06) mA cmECSA−2 compare favourably with the catalyst obtained by direct C‐interfacing the pristine Co8Pt4 cluster and with state‐of‐the‐art Pt/C reference catalysts. Our results demonstrate the potential of ZIF‐8‐mediated Pt−Co NP synthesis toward devising ORR catalysts with high Pt‐mass activity.
Continuous isomerisation of 2,5‐dimethylfuran to 2,4‐dimethylfuran over Ga‐silicate
Authors: Sauer Christopher , de Reijer Guido J. L. , Wilfinger Barbara , Hellman Anders , Carlsson Per‐Anders 
Published: February 2024

Abstract2,4‐dimethylfuran has a rare disubstitution pattern in the five‐membered heterocyclic furan ring that is highly interesting chemically but challenging to access synthetically. We present a heterogeneously catalysed route to synthesise 2,4‐dimethylfuran from commonly available 2,5‐dimethylfuran using a zeolite packed‐bed flow reactor. As supported by DFT calculations, the reaction occurs inside the zeolite channels, where the acid sites catalyse proton transfer followed by migration of a methyl group. The zeotype Ga‐silicate (MFI type) appears superior to an aluminium‐containing ZSM‐5 by demonstrating higher selectivities and slower catalyst deactivation. This work provides new opportunities for the continuous valorisation of bio‐feedstock molecules in the perspective of the emerging biorefinery era.
ALDH18A1‐related hereditary spastic paraplegia and developmental and epileptic encephalopathy with spike‐wave activation in sleep: Expanding the clinical phenotype
Authors: Ferrara Giusi , Cutillo Gianni , Peterlongo Irene , Minacapilli Eleonora , Iascone Maria , Veggiotti Pierangelo , Fiocchi Isabella 
Published: February 2024

AbstractObjectiveWe present the cases of two sisters, both harboring the same ALDH18A1 gene mutations, who presented with a complex clinical phenotype characterized by spastic paraparesis with ataxia, epileptic encephalopathy, severe psychomotor deficits, and behavioral abnormalities.MethodsCase description of two sisters with ALDH18A1 gene mutations.ResultsThe older patient, a 12‐year‐old girl, exhibited spastic paraparesis with ataxia, microcephaly, facial dysmorphisms, and severe intellectual disability, with an absence of verbal language. An electroencephalogram (EEG) revealed marked spike‐and‐wave activation during sleep (SWAS), although no clinically documented seizures were observed. The younger sister, who was 9 years old, displayed a similar clinical presentation, including spastic paraparesis with ataxia, microcephaly, dysmorphisms, however, she displayed slightly more severe intellectual deficits and polymorphic seizures. EEG revealed a SWAS pattern in this case. Magnetic resonance imaging scans in both cases showed only a thin corpus callosum. Whole exome sequencing unveiled the presence of two likely pathogenic variants in compound heterozygosity within the ALDH18A1 gene. Specifically, these variants included the splice site variant c.88 + 1c.88+1G>A of paternal origin and the variant c.1364c.1364T>C (p.Leu455Ser) of maternal origin. Both sisters displayed normal blood levels of ammonia, ornithine, citrulline, arginine, and other amino acids.InterpretationThese findings were compatible with ALDH18A1‐related HSP complicated with a clinical and EEG pattern reminiscent of DEE‐SWAS. We present the first report of DEE‐SWAS in ALDH18A1‐related HSP, expanding the clinical manifestations of this complex neurodevelopmental condition.