bluuebunny/crossref_metadata_2025_split huggingface dataset:
SYVN1-mediated ubiquitylation directs localization of MCT4 in the plasma membrane to promote the progression of lung adenocarcinoma
Authors: Zhao Meng , Huang Chen , Yang Lexin , Pan Boyu , Yang Shuting , Chang Jiao , Jin Yu , Zhao Gang , Yue Dongsheng , Qie Shuo , Ren Li 
Published: October 2023

AbstractTumour cells mainly generate energy from glycolysis, which is commonly coupled with lactate production even under normoxic conditions. As a critical lactate transporter, monocarboxylate transporter 4 (MCT4) is highly expressed in glycolytic tissues, such as muscles and tumours. Overexpression of MCT4 is associated with poor prognosis for patients with various tumours. However, how MCT4 function is post-translationally regulated remains largely unknown. Taking advantage of human lung adenocarcinoma (LUAD) cells, this study revealed that MCT4 can be polyubiquitylated in a nonproteolytic manner by SYVN1 E3 ubiquitin ligase. The polyubiquitylation facilitates the localization of MCT4 into the plasma membrane, which improves lactate export by MCT4; in accordance, metabolism characterized by reduced glycolysis and lactate production is effectively reprogrammed by SYVN1 knockdown, which can be reversed by MCT4 overexpression. Biologically, SYVN1 knockdown successfully compromises cell proliferation and tumour xenograft growth in mouse models that can be partially rescued by overexpression of MCT4. Clinicopathologically, overexpression of SYVN1 is associated with poor prognosis in patients with LUAD, highlighting the importance of the SYVN1-MCT4 axis, which performs metabolic reprogramming during the progression of LUAD.
A human monoclonal antibody binds within the poliovirus receptor-binding site to neutralize all three serotypes
Authors: Charnesky Andrew J. , Faust Julia E. , Lee Hyunwook , Puligedda Rama Devudu , Goetschius Daniel J. , DiNunno Nadia M. , Thapa Vaskar , Bator Carol M. , Cho Sung Hyun , Wahid Rahnuma , Mahmood Kutub , Dessain Scott , Chumakov Konstantin M. , Rosenfeld Amy , Hafenstein Susan L. 
Published: October 2023

AbstractGlobal eradication of poliovirus remains elusive, and it is critical to develop next generation vaccines and antivirals. In support of this goal, we map the epitope of human monoclonal antibody 9H2 which is able to neutralize the three serotypes of poliovirus. Using cryo-EM we solve the near-atomic structures of 9H2 fragments (Fab) bound to capsids of poliovirus serotypes 1, 2, and 3. The Fab-virus complexes show that Fab interacts with the same binding mode for each serotype and at the same angle of interaction relative to the capsid surface. For each of the Fab-virus complexes, we find that the binding site overlaps with the poliovirus receptor (PVR) binding site and maps across and into a depression in the capsid called the canyon. No conformational changes to the capsid are induced by Fab binding for any complex. Competition binding experiments between 9H2 and PVR reveal that 9H2 impedes receptor binding. Thus, 9H2 outcompetes the receptor to neutralize poliovirus. The ability to neutralize all three serotypes, coupled with the critical importance of the conserved receptor binding site make 9H2 an attractive antiviral candidate for future development.
SARS-CoV-2 genomic surveillance in wastewater as a model for monitoring evolution of endemic viruses
Authors: Yousif Mukhlid , Rachida Said , Taukobong Setshaba , Ndlovu Nkosenhle , Iwu-Jaja Chinwe , Howard Wayne , Moonsamy Shelina , Mhlambi Nompilo , Gwala Sipho , Levy Joshua I. , Andersen Kristian G. , Scheepers Cathrine , von Gottberg Anne , Wolter Nicole , Bhiman Jinal N. , Amoako Daniel Gyamfi , Ismail Arshad , Suchard Melinda , McCarthy Kerrigan 
Published: October 2023

AbstractAs global SARS-CoV-2 burden and testing frequency have decreased, wastewater surveillance has emerged as a key tool to support clinical surveillance efforts. The aims of this study were to identify and characterize SARS-CoV-2 variants in wastewater samples collected from urban centers across South Africa. Here we show that wastewater sequencing analyses are temporally concordant with clinical genomic surveillance and reveal the presence of multiple lineages not detected by clinical surveillance. We show that wastewater genomics can support SARS-CoV-2 epidemiological investigations by reliably recovering the prevalence of local circulating variants, even when clinical samples are not available. Further, we find that analysis of mutations observed in wastewater can provide a signal of upcoming lineage transitions. Our study demonstrates the utility of wastewater genomics to monitor evolution and spread of endemic viruses.
Creating resistance to avian influenza infection through genome editing of the ANP32 gene family
Authors: Idoko-Akoh Alewo , Goldhill Daniel H. , Sheppard Carol M. , Bialy Dagmara , Quantrill Jessica L. , Sukhova Ksenia , Brown Jonathan C. , Richardson Samuel , Campbell Ciara , Taylor Lorna , Sherman Adrian , Nazki Salik , Long Jason S. , Skinner Michael A. , Shelton Holly , Sang Helen M. , Barclay Wendy S. , McGrew Mike J. 
Published: October 2023

AbstractChickens genetically resistant to avian influenza could prevent future outbreaks. In chickens, influenza A virus (IAV) relies on host protein ANP32A. Here we use CRISPR/Cas9 to generate homozygous gene edited (GE) chickens containing two ANP32A amino acid substitutions that prevent viral polymerase interaction. After IAV challenge, 9/10 edited chickens remain uninfected. Challenge with a higher dose, however, led to breakthrough infections. Breakthrough IAV virus contained IAV polymerase gene mutations that conferred adaptation to the edited chicken ANP32A. Unexpectedly, this virus also replicated in chicken embryos edited to remove the entire ANP32A gene and instead co-opted alternative ANP32 protein family members, chicken ANP32B and ANP32E. Additional genome editing for removal of ANP32B and ANP32E eliminated all viral growth in chicken cells. Our data illustrate a first proof of concept step to generate IAV-resistant chickens and show that multiple genetic modifications will be required to curtail viral escape.
Fully automatic transfer and measurement system for structural superlubric materials
Authors: Chen Li , Lin Cong , Shi Diwei , Huang Xuanyu , Zheng Quanshui , Nie Jinhui , Ma Ming 
Published: October 2023

AbstractStructural superlubricity, a state of nearly zero friction and no wear between two contact surfaces under relative sliding, holds immense potential for research and application prospects in micro-electro-mechanical systems devices, mechanical engineering, and energy resources. A critical step towards the practical application of structural superlubricity is the mass transfer and high throughput performance evaluation. Limited by the yield rate of material preparation, existing automated systems, such as roll printing or massive stamping, are inadequate for this task. In this paper, a machine learning-assisted system is proposed to realize fully automated selective transfer and tribological performance measurement for structural superlubricity materials. Specifically, the system has a judgment accuracy of over 98% for the selection of micro-scale graphite flakes with structural superlubricity properties and complete the 100 graphite flakes assembly array to form various pre-designed patterns within 100 mins, which is 15 times faster than manual operation. Besides, the system is capable of automatically measuring the tribological performance of over 100 selected flakes on Si3N4, delivering statistical results for new interface which is beyond the reach of traditional methods. With its high accuracy, efficiency, and robustness, this machine learning-assisted system promotes the fundamental research and practical application of structural superlubricity.
Colossal negative magnetoresistance in field-induced Weyl semimetal of magnetic half-Heusler compound
Authors: Ueda Kentaro , Yu Tonghua , Hirayama Motoaki , Kurokawa Ryo , Nakajima Taro , Saito Hiraku , Kriener Markus , Hoshino Manabu , Hashizume Daisuke , Arima Taka-hisa , Arita Ryotaro , Tokura Yoshinori 
Published: October 2023

AbstractThe discovery of topological insulators and semimetals triggered enormous interest in exploring emergent electromagnetic responses in solids. Particular attention has been focused on ternary half-Heusler compounds, whose electronic structure bears analogy to the topological zinc-blende compounds while also including magnetic rare-earth ions coupled to conduction electrons. However, most of the research in this system has been in band-inverted zero-gap semiconductors such as GdPtBi, which still does not fully exhaust the large potential of this material class. Here, we report a less-studied member of half-Heusler compounds, HoAuSn, which we show is a trivial semimetal or narrow-gap semiconductor at zero magnetic field but undergoes a field-induced transition to a Weyl semimetal, with a negative magnetoresistance exceeding four orders of magnitude at low temperatures. The combined study of Shubnikov-de Haas oscillations and first-principles calculation suggests that the exchange field from Ho 4f moments reconstructs the band structure to induce Weyl points which play a key role in the strong suppression of large-angle carrier scattering. Our findings demonstrate the unique mechanism of colossal negative magnetoresistance and provide pathways towards realizing topological electronic states in a large class of magnetic half-Heusler compounds.
PdCu nanoalloy decorated photocatalysts for efficient and selective oxidative coupling of methane in flow reactors
Authors: Li Xiyi , Wang Chao , Yang Jianlong , Xu Youxun , Yang Yi , Yu Jiaguo , Delgado Juan J. , Martsinovich Natalia , Sun Xiao , Zheng Xu-Sheng , Huang Weixin , Tang Junwang 
Published: October 2023

AbstractMethane activation by photocatalysis is one of the promising sustainable technologies for chemical synthesis. However, the current efficiency and stability of the process are moderate. Herein, a PdCu nanoalloy (~2.3 nm) was decorated on TiO2, which works for the efficient, stable, and selective photocatalytic oxidative coupling of methane at room temperature. A high methane conversion rate of 2480 μmol g−1 h−1 to C2 with an apparent quantum efficiency of ~8.4% has been achieved. More importantly, the photocatalyst exhibits the turnover frequency and turnover number of 116 h−1 and 12,642 with respect to PdCu, representing a record among all the photocatalytic processes (λ > 300 nm) operated at room temperature, together with a long stability of over 112 hours. The nanoalloy works as a hole acceptor, in which Pd softens and weakens C-H bond in methane and Cu decreases the adsorption energy of C2 products, leading to the high efficiency and long-time stability.
Synthesis of piperidines and pyridine from furfural over a surface single-atom alloy Ru1CoNP catalyst
Authors: Qi Haifeng , Li Yurou , Zhou Zhitong , Cao Yueqiang , Liu Fei , Guan Weixiang , Zhang Leilei , Liu Xiaoyan , Li Lin , Su Yang , Junge Kathrin , Duan Xuezhi , Beller Matthias , Wang Aiqin , Zhang Tao 
Published: October 2023

AbstractThe sustainable production of value-added N-heterocycles from available biomass allows to reduce the reliance on fossil resources and creates possibilities for economically and ecologically improved synthesis of fine and bulk chemicals. Herein, we present a unique Ru1CoNP/HAP surface single-atom alloy (SSAA) catalyst, which enables a new type of transformation from the bio-based platform chemical furfural to give N-heterocyclic piperidine. In the presence of NH3 and H2, the desired product is formed under mild conditions with a yield up to 93%. Kinetic studies show that the formation of piperidine proceeds via a series of reaction steps. Initially, in this cascade process, furfural amination to furfurylamine takes place, followed by hydrogenation to tetrahydrofurfurylamine (THFAM) and then ring rearrangement to piperidine. DFT calculations suggest that the Ru1CoNP SSAA structure facilitates the direct ring opening of THFAM resulting in 5-amino-1-pentanol which is quickly converted to piperidine. The value of the presented catalytic strategy is highlighted by the synthesis of an actual drug, alkylated piperidines, and pyridine.
Development and validation of the pandemic fatigue scale
Authors: Lilleholt Lau , Zettler Ingo , Betsch Cornelia , Böhm Robert 
Published: October 2023

AbstractThe existence and nature of pandemic fatigue–defined as a gradually emerging subjective state of weariness and exhaustion from, and a general demotivation towards, following recommended health-protective behaviors, including keeping oneself informed during a pandemic–has been debated. Herein, we introduce the Pandemic Fatigue Scale and show how pandemic fatigue evolved during the COVID-19 pandemic, using data from one panel survey and two repeated cross-sectional surveys in Denmark and Germany (overall N = 34,582). We map the correlates of pandemic fatigue and show that pandemic fatigue is negatively related to people’s self-reported adherence to recommended health-protective behaviors. Manipulating the (de)motivational aspect of pandemic fatigue in a preregistered online experiment (N = 1584), we further show that pandemic fatigue negatively affects people’s intention to adhere to recommended health-protective behaviors. Combined, these findings provide evidence not only for the existence of pandemic fatigue, but also its psychological and behavioral associations.
Oxygen respiration and polysaccharide degradation by a sulfate-reducing acidobacterium
Authors: Dyksma Stefan , Pester Michael 
Published: October 2023

AbstractSulfate-reducing microorganisms represent a globally important link between the sulfur and carbon cycles. Recent metagenomic surveys expanded the diversity of microorganisms putatively involved in sulfate reduction underscoring our incomplete understanding of this functional guild. Here, we use genome-centric metatranscriptomics to study the energy metabolism of Acidobacteriota that carry genes for dissimilation of sulfur compounds in a long-term continuous culture running under alternating anoxic and oxic conditions. Differential gene expression analysis reveals the unique metabolic flexibility of a pectin-degrading acidobacterium to switch from sulfate to oxygen reduction when shifting from anoxic to oxic conditions. The combination of facultative anaerobiosis and polysaccharide degradation expands the metabolic versatility among sulfate-reducing microorganisms. Our results highlight that sulfate reduction and aerobic respiration are not mutually exclusive in the same organism, sulfate reducers can mineralize organic polymers, and anaerobic mineralization of complex organic matter is not necessarily a multi-step process involving different microbial guilds but can be bypassed by a single microbial species.
Achieving decent living standards in emerging economies challenges national mitigation goals for CO2 emissions
Authors: Huo Jingwen , Meng Jing , Zheng Heran , Parikh Priti , Guan Dabo 
Published: October 2023

AbstractEmerging economies, low- and middle-income countries experiencing rapid population and GDP growth, face the challenge of improving their living standards while stabilizing CO2 emissions to meet net-zero goals. In this study, we quantify the CO2 emissions required for achieving decent living standards (DLS) in emerging economies. The results show that, compared to other regions, achieving DLS in emerging Asian and African economies will result in more additional CO2 emissions, particularly in the DLS indicators of Mobility and Electricity. Achievement of DLS in emerging economies will result in 8.6 Gt of additional CO2 emissions, which should not jeopardize global climate targets. However, a concerning trend arises as more than half of the emerging economies (62 out of 121) will face substantial challenges in aligning their expected emission growth for achieving DLS with their national emission mitigation targets.
Positive regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in mice
Authors: Venit Tomas , Sapkota Oscar , Abdrabou Wael Said , Loganathan Palanikumar , Pasricha Renu , Mahmood Syed Raza , El Said Nadine Hosny , Sherif Shimaa , Thomas Sneha , Abdelrazig Salah , Amin Shady , Bedognetti Davide , Idaghdour Youssef , Magzoub Mazin , Percipalle Piergiorgio 
Published: October 2023

AbstractMetabolic reprogramming is one of the hallmarks of tumorigenesis. Here, we show that nuclear myosin 1 (NM1) serves as a key regulator of cellular metabolism. NM1 directly affects mitochondrial oxidative phosphorylation (OXPHOS) by regulating mitochondrial transcription factors TFAM and PGC1α, and its deletion leads to underdeveloped mitochondria inner cristae and mitochondrial redistribution within the cell. These changes are associated with reduced OXPHOS gene expression, decreased mitochondrial DNA copy number, and deregulated mitochondrial dynamics, which lead to metabolic reprogramming of NM1 KO cells from OXPHOS to aerobic glycolysis.This, in turn, is associated with a metabolomic profile typical for cancer cells, namely increased amino acid-, fatty acid-, and sugar metabolism, and increased glucose uptake, lactate production, and intracellular acidity. NM1 KO cells form solid tumors in a mouse model, suggesting that the metabolic switch towards aerobic glycolysis provides a sufficient carcinogenic signal. We suggest that NM1 plays a role as a tumor suppressor and that NM1 depletion may contribute to the Warburg effect at the onset of tumorigenesis.
Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke
Authors: Williamson Michael R. , Le Stephanie P. , Franzen Ronald L. , Donlan Nicole A. , Rosow Jill L. , Nicot-Cartsonis Mathilda S. , Cervantes Alexis , Deneen Benjamin , Dunn Andrew K. , Jones Theresa A. , Drew Michael R. 
Published: October 2023

AbstractStroke enhances proliferation of neural precursor cells within the subventricular zone (SVZ) and induces ectopic migration of newborn cells towards the site of injury. Here, we characterize the identity of cells arising from the SVZ after stroke and uncover a mechanism through which they facilitate neural repair and functional recovery. With genetic lineage tracing, we show that SVZ-derived cells that migrate towards cortical photothrombotic stroke in mice are predominantly undifferentiated precursors. We find that ablation of neural precursor cells or conditional knockout of VEGF impairs neuronal and vascular reparative responses and worsens recovery. Replacement of VEGF is sufficient to induce neural repair and recovery. We also provide evidence that CXCL12 from peri-infarct vasculature signals to CXCR4-expressing cells arising from the SVZ to direct their ectopic migration. These results support a model in which vasculature surrounding the site of injury attracts cells from the SVZ, and these cells subsequently provide trophic support that drives neural repair and recovery.
Photothermally heated colloidal synthesis of nanoparticles driven by silica-encapsulated plasmonic heat sources
Authors: Biswas Aritra , Lemcoff Nir , Shelonchik Ofir , Yesodi Doron , Yehezkel Elad , Finestone Ella Yonit , Upcher Alexander , Weizmann Yossi 
Published: October 2023

AbstractUsing photons to drive chemical reactions has become an increasingly important field of chemistry. Plasmonic materials can provide a means to introduce the energy necessary for nucleation and growth of nanoparticles by efficiently converting visible and infrared light to heat. Moreover, the formation of crystalline nanoparticles has yet to be included in the extensive list of plasmonic photothermal processes. Herein, we establish a light-assisted colloidal synthesis of iron oxide, silver, and palladium nanoparticles by utilizing silica-encapsulated gold bipyramids as plasmonic heat sources. Our work shows that the silica surface chemistry and localized thermal hotspot generated by the plasmonic nanoparticles play crucial roles in the formation mechanism, enabling nucleation and growth at temperatures considerably lower than conventional heating. Additionally, the photothermal method is extended to anisotropic geometries and can be applied to obtain intricate assemblies inaccessible otherwise. This study enables photothermally heated nanoparticle synthesis in solution through the plasmonic effect and demonstrates the potential of this methodology.
Gottesman-Kitaev-Preskill qubit synthesizer for propagating light
Authors: Takase Kan , Fukui Kosuke , Kawasaki Akito , Asavanant Warit , Endo Mamoru , Yoshikawa Jun-ichi , van Loock Peter , Furusawa Akira 
Published: October 2023

AbstractPractical quantum computing requires robust encoding of logical qubits in physical systems to protect fragile quantum information. Currently, the lack of scalability limits the logical encoding in most physical systems, and thus the high scalability of propagating light can be a game changer. However, propagating light also has difficulty in logical encoding due to weak nonlinearity. Here, we propose a synthesizer that encodes Gottesman-Kitaev-Preskill (GKP) qubits in propagating light by exploiting the nonlinearity of photon detectors. This synthesizer is based on an approach what we call Gaussian breeding, leading to the following four advantages: (i) systematic and rigorous synthesis of arbitrary GKP qubits, (ii) use of minimal resources, (iii) high fidelity and high success probability, and (iv) robustness against loss. There has been no protocol that incorporates all these advantages, and thus the proposed synthesizer excels in both performance and feasibility. By employing our method, one can generate GKP qubits using a few to several squeezed light sources, beam splitters and photon detectors.
Large anomalous Hall effect and unusual domain switching in an orthorhombic antiferromagnetic material NbMnP
Authors: Kotegawa Hisashi , Kuwata Yoshiki , Huyen Vu Thi Ngoc , Arai Yuki , Tou Hideki , Matsuda Masaaki , Takeda Keiki , Sugawara Hitoshi , Suzuki Michi-To 
Published: October 2023

AbstractSpecific antiferromagnetic (AF) spin configurations generate large anomalous Hall effects (AHEs) even at zero magnetic field through nonvanishing Berry curvature in momentum space. In addition to restrictions on AF structures, suitable control of AF domains is essential to observe this effect without cancellations among its domains; therefore, compatible materials remain limited. Here we show that an orthorhombic noncollinear AF material, NbMnP, acquired AF structure-based AHE and controllability of the AF domains. Theoretical calculations indicated that a large Hall conductivity of ~230 Ω−1cm−1 originated from the AF structure of NbMnP. Symmetry considerations explained the production of a small net magnetization, whose anisotropy enabled the generation and cancellation of the Hall responses using magnetic fields in different directions. Finally, asymmetric hysteresis in NbMnP shows potential for the development of controllability of responses in AF materials.