bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross‐typic specificity and immunotherapeutic potential of a human HPV16 E7‐specific CTL line
Authors: Youde Sarah J. , McCarthy Corinna M. , Thomas Karen J. , Smith Kelly L. , Man Stephen 
Published: December 2004

AbstractCervical cancer (CaCx) is strongly associated with human papillomavirus (HPV) infection, particularly HPV types 16 and 18. The constitutive expression of HPV E6 and E7 proteins in CaCx makes them attractive targets for CTL based immunotherapy. However cervical carcinomas may have features, e.g., antigen processing defects, that limit the effectiveness of HPV specific CTL. Furthermore most vaccine development has concentrated on HPV type 16, and it is not clear whether such vaccines could induce CTL able to cross‐react on related oncogenic HPV types, e.g., HPV31 and 52. To investigate these potentially important parameters in vitro, we used a CTL (D4) specific for HPV16 E711–20. D4 was able to kill a variety of HPV16+ CaCx cell lines including those with suspected (CaSki) or known antigen processing defects (C33A), and with low HPV DNA copy number (SiHa). D4 was also able to cross react on a related peptide from HPV52 E7 but not HPV31 E7. Further analysis suggested that D4 cross reactivity against related peptides was influenced both by TCR contact residues and a certain threshold for peptide binding. The HPV cross‐reactivity was confirmed at the whole protein level as D4 was also able to recognize the endogenously processed forms of HPV16 and 52 E7 but not 31 E7. These results suggest that HPV16 E711–20 would be a useful epitope for immunotherapy in both HPV 16 and 52 tumours. Despite this, it is difficult to generate these CTL in response to vaccination, emphasizing the need for definition of novel epitopes and more efficient vaccination strategies. © 2005 Wiley‐Liss, Inc.
E‐cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: A comparison of E‐cadherin with desmosomal components
Authors: Bosch Franz X. , Andl Claudia , Abel Ulrich , Kartenbeck Jürgen 
Published: December 2004

AbstractE‐cadherin‐mediated and desmosomal cell‐cell adhesion have been implicated in the suppression of invasive and metastatic behavior of squamous cell carcinomas. Whether the adhaerens junction represented by E‐cadherin and the desmosomes interplay or have distinct and separate roles in squamous cell cancer progression is still unclear. We have studied a cohort of 200 primary tumors and 56 lymph node metastases from different anatomic sites of the head and neck region for changes in synthesis of E‐cadherin, desmoplakin and desmoglein by immunohistochemistry (IHC). Selected cases were studied by indirect immunofluorescence (IIF) and electron microscopy (EM). Only frozen sections were evaluated since they gave stronger and reproducible staining results. IHC data obtained were compared to clinical parameters. While some reduction in immunostaining was found in virtually all invasive tumors, at least partial expression, including that of E‐cadherin, persisted in most late stage tumors and in lymph node metastases. Reduced desmosomal staining correlated with desmosomes reduced in numbers, size or in structural defects by EM analysis. By univariate analysis, reduction in synthesis of both E‐cadherin and the desmosomal components that were generally linked (i.e., they showed positive rank correlations) were significantly associated with clinical parameters including overall and disease‐free survival. However, by multivariate analysis including a Cox proportional hazards regression model (backward selection), the desmosomal components were not significant as independent prognostic factors. By contrast, E‐cadherin was strongly associated with patient prognosis. In line with the highly significant association of reduced E‐cadherin synthesis with an increased relative risk of follow up events, i.e., regional lymph node (p = 0.0007) and distant metastasis (p < 0.0001), as well as local recurrences (p < 0.0001), the prognostic strength of E‐cadherin was independent of and stronger than histological grading, N stage, tumor site, and even stronger than the TNM stage. Based on these results, evaluation of E‐cadherin in squamous cell carcinomas by immunostaining is recommended as a significant prognostic marker. © 2004 Wiley‐Liss, Inc.
Association of amino acid substitution polymorphisms in DNA repair genes TP53, POLI, REV1 and LIG4 with lung cancer risk
Authors: Sakiyama Tokuki , Kohno Takashi , Mimaki Sachiyo , Ohta Tsutomu , Yanagitani Noriko , Sobue Tomotaka , Kunitoh Hideo , Saito Ryusei , Shimizu Kimiko , Hirama Chie , Kimura Junko , Maeno Go , Hirose Hiroshi , Eguchi Takashi , Saito Daizo , Ohki Misao , Yokota Jun 
Published: December 2004

AbstractSingle nucleotide polymorphisms (SNPs) were searched for in 36 genes involved in diverse DNA repair pathways, and 50 nonsynonymous (associated with amino acid changes) SNPs identified were assessed for associations with lung cancer risk by a case‐control study consisting of 752 adenocarcinoma cases, 250 squamous cell carcinoma cases and 685 controls. An SNP, Arg72Pro, of the TP53 gene encoding a DNA damage response protein showed the strongest association with squamous cell carcinoma risk (OR Pro/Pro vs. Arg/Arg = 2.2), while 2 other SNPs, Phe257Ser of the REV gene encoding a translesion DNA polymerase and Ile658Val of the LIG4 gene encoding a DNA double‐strand break repair protein, also showed associations (OR Ser/Ser vs. Phe/Phe = 2.0 and OR Ile/Val vs. Ile/Ile = 0.4, respectively). An SNP, Thr706Ala, in the POLI gene encoding another translesion DNA polymerase was associated with adenocarcinoma and squamous cell carcinoma risk, particularly in individuals of ages < 61 years (OR Ala/Ala + Ala/Thr vs. Thr/Thr = 1.5 and 2.4, respectively). POLI is the human counterpart of PolI, a strong candidate for the Par2 (pulmonary adenoma resistance 2) gene responsible for adenoma/adenocarcinoma susceptibility in mice. The present results suggest that these 4 SNPs function as genetic factors underlying lung cancer susceptibility by modulating activities to maintain the genome integrity of each individual. © 2004 Wiley‐Liss, Inc.
Bowes melanoma cells secrete heregulin, which can promote aggregation and counteract invasion of human mammary cancer cells
Authors: Stove Christophe , Boterberg Tom , Van Marck Veerle , Mareel Marc , Bracke Marc 
Published: December 2004

AbstractInvasiveness, the ability of cancer cells to migrate beyond the normal tissue boundaries, often leads to metastasis and thereby usually turns cancer into a fatal disease. At the molecular level, the E‐cadherin/catenin complex is an example of a powerful invasion suppressor in epithelial cells. Since the absence of melanocytes has been associated with disturbances in epithelial organization, we decided to investigate the influence of molecules secreted by melanocytes on the function of the E‐cadherin/catenin complex. We used the Bowes melanoma cell line as a source of such molecules. The conditioned medium of Bowes melanoma stimulated aggregation of human MCF‐7/6 mammary adenocarcinoma cells at short (30 min) and long (24–72 hr) notice. This effect could be inhibited by MB2, an antibody against human E‐cadherin. Conditioned medium of Bowes melanoma also inhibited invasion of MCF‐7/6 cells into precultured chick heart fragments. Candidate molecules such as insulin, insulin‐like growth factor I, follistatin and interleukins were ruled out to be responsible for the effects, but heregulin mimicked some of the effects of the conditioned medium. Our data indicate that heregulin stimulates aggregation and inhibits invasion of MCF‐7/6 cells via activation of the E‐cadherin/catenin complex. © 2005 Wiley‐Liss, Inc.
Naturally processed and HLA‐B8‐presented HPV16 E7 epitope recognized by T cells from patients with cervical cancer
Authors: Oerke Sebastian , Höhn Hanni , Zehbe Ingeborg , Pilch Henryk , Schicketanz Karl‐Heinz , Hitzler Walter E. , Neukirch Claudia , Freitag Kirsten , Maeurer Markus J. 
Published: December 2004

AbstractSeveral major histocompatibility complex (MHC) alleles have been reported to present peptides derived from the HPV16 E7 oncoprotein to T cells. We describe an overrepresentation of the HLA‐B8 allele (28.44%) in cervical cancer patients as compared to the MHC class I allele frequency in a local healthy control population (18.80%) and the identification of an HLA‐B8‐binding peptide TLHEYMLDL (HPV16 E77–15), which is able to drive HPV16 E7‐specific and MHC class I‐restricted T‐cell responses in peripheral blood lymphocytes from healthy individuals. TLHEYMLDL‐specific T cells recognize the naturally processed and presented peptide on HPV16+ cervical cancer cells transfected with the HLA‐B8 gene defined by IFN‐γ production. This peptide epitope is also recognized by freshly harvested tumor‐infiltrating T cells or T cells from tumor‐draining lymph nodes from patients with cervical cancer determined by flow cytometry as well as by tetramer in situ staining. HLA‐B8‐restricted HPV E77–15‐specific T cells reside predominantly in the CD8+ CD45RA+ CCR7+ precursor or in the differentiated CD8+ CD45RA+ CCR7− T‐cell population. © 2004 Wiley‐Liss, Inc.
p‐Adic Colombeau‐Egorov type theory of generalized functions
Authors: Albeverio S. , Khrennikov A. Yu. , Shelkovich V. M. 
Published: December 2004

AbstractThe p‐adic Colombeau‐Egorov algebra of generalized functions on ℚnp is constructed. For generalized functions the operations of multiplication, Fourier‐transform, convolution, taking pointvalues are defined. The operations of (fractional) partial differentiation and (fractional) partial integration are introduced by the Vladimirov's pseudodifferential operator. The products of Bruhat‐Schwartz distributions are well defined as elements of this algebra. In contrast to the “usual” Colombeau and Egorov ℂ‐theories, where generalized functions on ℝn are not determined by their pointvalues on ℝn, p‐adic Colombeau‐Egorov generalized functions are uniquely determined by their pointvalues on ℚnp. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Low degree 3‐folds in P6
Authors: Bertolini Marina , Fania Maria Lucia 
Published: December 2004

AbstractIn this paper we construct new smooth varieties of dimension 3 in P6, with degree 12 ≤ d ≤ 15 and we also give different constructions for some known varieties. Moreover we determine the adjunction theoretic structure of all the varieties that we deal with. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Generalization of a class of nonlinear averaging integral operators
Authors: Bulboacă Teodor 
Published: December 2004

AbstractLet H(U) be the space of all analytic functions in the unit disk U, and let coE denote the convex hull of the set E ⊂ ℂ. If K ⊂ H(U) then the operator I : K → H(U) is said to be an averaging operator if

For a function h ∈ A ⊂ H(U) we will determine simple sufficient conditions on h such that

for all f ∈ ℳ︁′1/β, where

and ℳ︁′1/β represents the class of 1/β‐convex functions (not necessarily normalized).As an application, we will give sufficient conditions on h to insure that the operators Ih;β,γ are averaging operators on certain subsets of H(U), in order to generalize the result of [5]. In addition, some particular cases of this result obtained for appropriate choices of the function h will also be given. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Witten triples and the Seiberg–Witten equations on a complex surface
Authors: Dürr Markus 
Published: December 2004

AbstractWe study the solutions of the Seiberg–Witten equations on complex surfaces. We show that for a large class of parameters, the gauge equivalence classes of irreducible solutions of the twisted Seiberg–Witten equations correspond to stable Witten triples. We prove that on Kähler surfaces this correspondence is the set‐theoretical support of an isomorphism of real‐analytic spaces. This makes it possible to take multiplicities into account and generalizes and unifies results previously obtained by Witten. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Limiting reiteration for real interpolation with slowly varying functions
Authors: Gogatishvili Amiran , Opic Bohumír , Trebels Walter 
Published: December 2004

AbstractWe present reiteration formulae with limiting values θ = 0 and θ = 1 for a real interpolation method involving slowly varying functions. Applications to the Lorentz–Karamata spaces, the Fourier transform and the Riesz potential are given. In particular, our results yield improvements of limiting Sobolev‐type embeddings due to Trudinger, Hansson, Brézis and Wainger, Edmunds, Gurka and Opic, Fusco, Lions and Sbordone, et al., and they are related to those of Edmunds, Kerman and Pick, or Pustylnik. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Effective finiteness theorems for maps between canonically polarizedcompact complex manifolds
Authors: Heier Gordon 
Published: December 2004

AbstractEffective bounds for the finite number of surjective holomorphic maps between canonically polarized compact complex manifolds of any dimension with fixed domain are proven. Both the case of a fixed target and the case of varying targets are treated. In the case of varying targets, bounds on the complexity of Chow varieties are used. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Oscillation criteria for nonlinear differential equations with integrable coefficient
Authors: Hong Huei‐Lin , Yeh Cheh‐Chih , Hong Chen‐Huang 
Published: December 2004

AbstractIn this paper, we consider the oscillation of the nonlinear differential equation

We obtain a new sufficient condition for any nonoscillatory solution y(t) of the above equation satisfying lim inft→∞ |y(t)| = 0. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Comparative analysis of HIV‐1 Tat variants
Authors: Pantano Sergio , Carloni Paolo 
Published: December 2004

AbstractHIV‐1 Tat protein is a crucial element for viral replication; therefore, its inhibition might be exploited against the AIDS infection. To gain insights on the natural variability of this protein, we present a comparative investigation on the relationship between the primary sequences and the experimentally available three‐dimensional structures from the HIV‐1 Tat variants Z2, BRU, and MAL. Our computational tools include sequence conservation algorithms, structural analysis, electrostatic modeling, and molecular dynamics (MD) simulations. We find that two regions located between residues 10–18 and 41–52 display the highest primary sequence conservation, while the most conserved region among the available structures corresponds approximately to the segment between positions ∼44 and 50. Furthermore, in spite of their large structural divergence, Tat variants share a common mode for long‐range intramolecular interactions. Finally, the flexibility of the Z2, BRU, and MAL variants, as emerging from multinanosecond MD simulations, is rather similar. Based on this work, we conclude that the turnlike region between amino acids 44 and 50 is structurally most conserved, emerging as an important motif for pharmaceutical targeting aimed toward inhibiting Tat action. Proteins 2005. © 2004 Wiley‐Liss, Inc.
FAST: A novel protein structure alignment algorithm
Authors: Zhu Jianhua , Weng Zhiping 
Published: December 2004

AbstractWe present a novel algorithm named FAST for aligning protein three‐dimensional structures. FAST uses a directionality‐based scoring scheme to compare the intra‐molecular residue–residue relationships in two structures. It employs an elimination heuristic to promote sparseness in the residue‐pair graph and facilitate the detection of the global optimum. In order to test the overall accuracy of FAST, we determined its sensitivity and specificity with the SCOP classification (version 1.61) as the gold standard. FAST achieved higher sensitivities than several existing methods (DaliLite, CE, and K2) at all specificity levels. We also tested FAST against 1033 manually curated alignments in the HOMSTRAD database. The overall agreement was 96%. Close inspection of examples from broad structural classes indicated the high quality of FAST alignments. Moreover, FAST is an order of magnitude faster than other algorithms that attempt to establish residue–residue correspondence. Typical pairwise alignments take FAST less than a second with a Pentium III 1.2GHz CPU. FAST software and a web server are available at http://biowulf.bu.edu/FAST/. Proteins 2005. © 2004 Wiley‐Liss, Inc.
Protein conformational transitions coupled to binding in molecular recognition of unstructured proteins: Deciphering the effect of intermolecular interactions on computational structure prediction of the p27Kip1 protein bound to the cyclin A–cyclin‐dependent kinase 2 complex
Authors: Verkhivker Gennady M. 
Published: December 2004

AbstractThe relationship between folding mechanism coupled to binding and structure prediction of the tertiary complexes is studied for the p27Kip1 protein which has an intrinsically disordered unbound form and undergoes a functional folding transition during complex formation with the phosphorylated cyclin A–cyclin‐dependent kinase 2 (Cdk2) binary complex. Hierarchy of p27Kip1 structural loss determined in our earlier studies from temperature–induced Monte Carlo simulations and subsequent characterization of the transition state ensemble (TSE) for the folding reaction have shown that simultaneous ordering of the p27Kip1 native intermolecular interface for the β‐hairpin and β‐strand secondary structure elements is critical for nucleating a rapid kinetic transition to the native tertiary complex. In the present study, we investigate the effect of forming specific intermolecular interactions on structure prediction of the p27Kip1 tertiary complex. By constraining different secondary structure elements of p27Kip1 in their native bound conformations and conducting multiple simulated annealing simulations, we analyze differences in the success rate of predicting the native structure of p27Kip1 in the tertiary complex. In accordance with the nucleation–condensation mechanism, we have found that further stabilization of the native intermolecular interface for the β‐hairpin and β‐strand elements of p27Kip1, that become ordered in the TSE, but are hardly populated in the unbound state, results in a consistent acquisition of the native bound structure. Conversely, the excessive stablization of the local secondary structure elements, which are rarely detected in the TSE, has a detrimental effect on convergence to the native bound structure. Proteins 2005. © 2004 Wiley‐Liss, Inc.
Implicit solvent simulations of peptide interactions with anionic lipid membranes
Authors: Lazaridis Themis 
Published: December 2004

AbstractA recently developed implicit membrane model (IMM1) is supplemented with a Gouy–Chapman term describing counterion‐screened electrostatic interactions of a solute with negatively charged membrane lipids. The new model is tested on peptides that bind to anionic membranes. Pentalysine binds just outside the plane of negative charge, whereas Lys‐Phe peptides insert their aromatic rings into the hydrophobic core. Melittin and magainin 2 bind more strongly to anionic than to neutral membranes and in both cases insert their hydrophobic residues into the hydrocarbon core. The third domain of Antennapedia homeodomain (penetratin) binds as an α‐helix in the headgroup region. Cardiotoxin II binds strongly to anionic membranes but marginally to neutral ones. In all cases, the location and configuration of the peptides are consistent with experimental data, and the effective energy changes upon binding compare favorably with experimental binding free energies. The model opens the way to exploring the effect of membrane charge on the location, conformation, and dynamics of a large variety of biologically active peptides on membranes. Proteins 2005. © 2004 Wiley‐Liss, Inc.
A Novel Polyoxovanadium Cluster Shell: β‐As8V14O42
Authors: Zheng Shou‐Tian , Zhang Jie , Yang Guo‐Yu 
Published: December 2004

AbstractThe hydrothermal reaction of VOSO4, As2O5, piperazine and H2O produces [H2N(CH2)4NH2]4[β‐As8V14O42(SO4)]·2HSO4 (1), which is the first arsenic‐vanadium cluster containing a spherical β‐As8V14O42 shell. The structure of this compound was characterized by single crystal X‐ray diffraction, elemental analysis, TG, and IR spectrum. Crystal data for 1: Orthorhombic, Cmcm, a = 15.369(1) Å, b = 16.404(1) Å, c = 25.772(1) Å, V = 6497.4(9) Å3, Z = 4.
Synthesen und Kristallstrukturen neuer sulfidoverbrückter Rutheniumclusterverbindungen
Authors: Wunder Markus , Eckermann Amanda L. , Rauchfuss Thomas B. , Fenske Dieter 
Published: December 2004

AbstractDurch die Reaktion von S(SiMe3)2 bzw. NaSH mit [RuCl2(PPh3)3], bzw. [Ru3Cl8(PEt3)4] können sulfidoverbrückte Rutheniumclusterverbindungen dargestellt werden. Im folgenden werden die Verbindungen [Ru6S8(PPh3)6][PF6] (1), [Ru6S8(PPh3)6][RuCl4(PPh3)2] (2), [Ru6S8(PEt3)6] (3) und [Ru3S4Cl2(PPh3)3]2 (4) beschrieben. Die Strukturen dieser Verbindungen wurden mittels Einkristall‐Röntgenstrukturanalyse aufgeklärt.
Economic implications of pain management
Authors: Loeser J. D. 
Published: December 2004

Background: Economic issues in pain management affect the patient, the provider and society. This paper will review some of the data on the costs to society of chronic pain and its associated disability. It will also look at the costs to patient and provider of alternative economic models. Conceptual issues that underlie health care delivery and the attendant costs must be addressed if society is to gain control over runaway health care costs and reduce the economic burden of chronic pain and disability for the patient as well as the provider.Methods: Literature review and synthesis.Results: Chronic pain is the primary cause of health care consumption and disability in the working years. Multidisciplinary pain clinics have proven utility. Data on efficacy of most other kinds of care is lacking. Disability costs are related to conceptual inadequacies and the medicalization of post‐industrial societies.Conclusion: To control inappropriate care and escalating costs, we must change concepts of pain and disability and the methods of funding both of these in relation to chronic pain. The outcome of the continuing struggle between the profession of medicine, the state and capitalists will determine how and whether pain management is a part of medical care.
Elevation of interleukin‐6 levels in cervical secretions as a predictor of preterm delivery
Authors: Lange Melanie , Chen Frank K. , Wessel Jens , Buscher Ulrich , Dudenhausen Joachim W. 
Published: December 2004

Background. The objective of this study was to evaluate the predictive value of interleukin‐6 (IL‐6) in cervical secretions for preterm delivery before 34+0 gestational weeks in patients with preterm labor.Methods. In this prospective study, 31 patients were enrolled with preterm labor and intact membranes. Cervical swabs were taken on admission and the concentration of IL‐6 was measured. The gestational age at the time of admission and delivery, as well as the time interval to delivery, were evaluated and compared with the concentration of IL‐6 on admission.Results. Cervical IL‐6 was significantly elevated in the preterm delivery group (< 34+0 gestational weeks, p < 0.05). Choosing 20 pg/ml as a cut‐off concentration, cervical IL‐6 had a sensitivity and negative predictive value of 100%, a specificity of 67% and a positive predictive value of 47%. Concerning the time interval to delivery, the sensitivity of IL‐6 was 100% and the specificity 57% for delivery within 2 days and 100% and 64% for delivery within 7 days.Conclusions. Cervical IL‐6 is a new, promising diagnostic tool to identify patients at high risk of preterm delivery.