bluuebunny/crossref_metadata_2025_split huggingface dataset:
COMPASS core subunits MpSet1 and MpSwd3 regulate Monascus pigments synthesis in Monascus purpureus
Authors: Wu Zhongling , Gao Hongyan , Liu Zhenmin 
Published: February 2024

AbstractIn eukaryotes, methylation of histone H3 at lysine 4 (H3K4me) catalyzed by the complex of proteins associated with Set1 (COMPASS) is crucial for the transcriptional regulation of genes and the development of organisms. In Monascus, the functions of COMPASS in establishing H3K4me remain unclear. This study first identified the conserved COMPASS core subunits MpSet1 and MpSwd3 in Monascus purpureus and confirmed their roles in establishing H3K4me2/3. Loss of MpSet1 and MpSwd3 resulted in slower growth and development and inhibited the formation of cleistothecia, ascospores, and conidia. The loss of these core subunits also decreased the production of extracellular and intracellular Monascus pigments (MPs) by 94.2%, 93.5%, 82.7%, and 82.5%, respectively. In addition, RNA high‐throughput sequencing and quantitative real‐time polymerase chain reaction (qRT‐PCR) showed that the loss of MpSet1 and MpSwd3 altered the expression of 2646 and 2659 genes, respectively, and repressed the transcription of MPs synthesis‐related genes. In addition, the ΔMpset1 and ΔMpswd3 strains demonstrated increased sensitivity to cell wall stress with the downregulation of chitin synthase‐coding genes. These results indicated that the COMPASS core subunits MpSet1 and MpSwd3 help establish H3K4me2/3 for growth and development, spore formation, and pigment synthesis in Monascus. These core subunits also assist in maintaining cell wall integrity.
Use of olive and sunflower protein hydrolysates for the physical and oxidative stabilization of fish oil‐in‐water emulsions
Authors: Ospina‐Quiroga Jeimmy Lizeth , Coronas‐Lozano Cristina , García‐Moreno Pedro Jesús , Guadix Emilia María , Almécija‐Rodríguez María del Carmen , Pérez‐Gálvez Raúl 
Published: February 2024

AbstractBACKGROUNDOlive and sunflower seeds are by‐products generated in large amounts by the plant oil industry. The technological and biological properties of plant‐based substrates, especially protein hydrolysates, have increased their use as functional ingredients for food matrices. The present study evaluates the physical and oxidative stabilities of 50 g kg−1 fish oil‐in‐water emulsions where protein hydrolysates from olive and sunflower seeds were incorporated at 20 g kg−1 protein as natural emulsifiers. The goal was to investigate the effect of protein source (i.e. olive and sunflower seeds), enzyme (i.e. subtilisin and trypsin) and degree of hydrolysis (5%, 8% and 11%) on the ability of the hydrolysate to stabilize the emulsion and retard lipid oxidation over a 7‐day storage period.RESULTSThe plant protein hydrolysates displayed different emulsifying and antioxidant capacities when incorporated into the fish oil‐in‐water emulsions. The hydrolysates with degrees of hydrolysis (DH) of 5%, especially those from sunflower seed meal, provided higher physical stability, regardless of the enzymatic treatment. For example, the average D [2, 3] values for the emulsions containing sunflower subtilisin hydrolysates at DH 5% only slightly increased from 1.21 ± 0.02 μm (day 0) to 2.01 ± 0.04 μm (day 7). Moreover, the emulsions stabilized with sunflower or olive seed hydrolysates at DH 5% were stable against lipid oxidation throughout the storage experiment, with no significant variation in the oxidation indices between days 0 and 4.CONCLUSIONThe results of the present study support the use of sunflower seed hydrolysates at DH 5% as natural emulsifiers for fish oil‐in‐water emulsions, providing both physical and chemical stability against lipid oxidation. © 2024 Society of Chemical Industry.
Women show a positive response to platelet‐rich plasma despite presenting more painful knee osteoarthritis than men
Authors: Sánchez Mikel , Jorquera Cristina , López de Dicastillo Leonor , Martínez Nina , Espregueira‐Mendes João , Vergés Josep , Azofra Juan , Delgado Diego 
Published: February 2024

AbstractPurposeThe purpose of this study was to evaluate the impact of gender on the efficacy of platelet‐rich plasma (PRP) in patients with knee osteoarthritis (KOA), comparing their short‐term response between men and women.MethodsFour hundred‐eighteen patients (529 knees) were included. Patients were treated with three injections of PRP on a weekly basis. Blood and PRP samples were randomly tested. Patients were asked to complete the knee injury and osteoarthritis outcome score (KOOS) and 12‐item short form survey (SF‐12), at baseline and 6 months. Success rates were calculated according to a reduction in the pain score of at least 9.3 points [minimal clinically important improvement (MCII)]. Comparative tests and multivariate regression were performed.ResultsThe PRP had a platelet concentration factor of 2.0X compared to blood levels, with no leucocytes or erythrocytes. KOOS scores showed an increase from baseline to 6 months (p < 0.0001). There was an increase in the physical component summary (PCS) (p < 0.0001) and mental component summary (MCS) (p < 0.01) of the SF‐12. The number of knees of women with MCII was 156 out of 262 (59.6%), whereas the number of knees of men was 136 out of 267 (50.9%) (p = 0.0468). Women had worse baseline scores on pain (p = 0.009), PCS (p < 0.0001) and MCS (p < 0.0001).ConclusionAlthough the symptomatology generated by KOA was worse in women when compared to men, treatment with repeated injections of PRP was effective, ultimately achieving a higher improvement in women providing comparable final follow‐up outcomes between men and women.Level of EvidenceLevel IV
An Integrative Model of ENT Healthcare for the Homeless Population
Authors: Patel Evan A. , Shah Swapnil V. , Poulson Trevor A. , Jagasia Ashok A. 
Published: February 2024

ObjectiveOur aim was to determine the need for otolaryngology care within the homeless population, identify barriers to access that the homeless population may face, and develop a model system which would address these needs with respect to the barriers.MethodsA retrospective chart review of 812 patients seen between 1/25/16–3/21/2020 was performed. Charts were obtained from homeless patients seen at free general clinics held shelters in Chicago, IL (781 charts) and in Champaign, IL (31 charts). Records reporting at least one otolaryngology disease in a patient experiencing homelessness were included in this study. Patients were considered homeless if they resided at the shelter at the time of their appointment. To determine common barriers to care, a simple yes/no questionnaire was administered to residents at west‐side Chicago homeless shelters. Questions addressed barriers to health care access that had been mentioned by patients seen at free clinics.ResultsChart review findings demonstrated that 14.3% (n = 142) of all homeless patients seen at free in‐shelter clinics were seen for ENT‐related disorders. Survey results revealed that 76.3% (n = 71) of respondents believed that telemedicine services would be useful in shelters. 74.2% (n = 69) stated they were unable to afford prescribed medications. 93.5% (n = 87) stated that better access to transportation would increase their likelihood of seeking care.ConclusionsIn our attempt to bridge this inequity, we have launched a hybrid in‐person/virtual care program to improve access to otolaryngology care for the homeless community.Level of EvidenceN/A Laryngoscope, 134:2705–2709, 2024
Comprehensive analysis of m6A methylome and transcriptome by Nanopore sequencing in clear cell renal carcinoma
Authors: Li Hexin , Li Chang , Zhang Yuxiang , Jiang Weixing , Zhang Fubo , Tang Xiaokun , Sun Gaoyuan , Xu Siyuan , Dong Xin , Shou Jianzhong , Yang Yong , Chen Meng 
Published: February 2024

AbstractN6‐methyladenosine (m6A) is the most prevalent epigenetic modification on eukaryotic messenger RNAs. Recent studies have focused on elucidating the key role of m6A modification patterns in tumor progression. However, the relationship between m6A and transcriptional regulation remains elusive. Nanopore technology enables the quantification of m6A levels at each genomic site. In this study, a pair of tumor tissues and adjacent normal tissues from clear cell renal cell carcinoma (ccRCC) surgical samples were collected for Nanopore direct RNA sequencing. We identified 9644 genes displaying anomalous m6A modifications, with 5343 genes upregulated and 4301 genes downregulated. Among these, 5224 genes were regarded as dysregulated genes, encompassing abnormal regulation of both m6A modification and RNA expression. Gene Set Enrichment Analysis revealed an enrichment of these genes in pathways related to renal system progress and fatty acid metabolic progress. Furthermore, the χ2 test demonstrated a significant association between the levels of m6A in dysregulated genes and their transcriptional expression levels. Additionally, we identified four obesity‐associated genes (FTO, LEPR, ADIPOR2, and NPY5R) among the dysregulated genes. Further analyses using public databases revealed that these four genes were all related to the prognosis and diagnosis of ccRCC. This study introduced the novel approach of employing conjoint analysis of m6A modification and RNA expression based on Nanopore sequencing to explore potential disease‐related genes. Our work demonstrates the feasibility of the application of Nanopore sequencing technology in RNA epigenetic regulation research and identifies new potential therapeutic targets for ccRCC.
Simplified method for evaluating tunnel response induced by a new tunnel excavation underneath
Authors: Feng Guohui , Xu Changjie , Ding Zhi , Liang Luju , Li Yujie , Chi Minliang 
Published: February 2024

AbstractTo estimate the tunnel response induced by a new tunnel excavation underneath, theoretical solutions are proposed in this study. The overlying tunnel is idealized as an infinite Timoshenko beam resting on the Kerr foundation model, then the vertical force balance equation is established. The unloading stress can be expressed as Fourier cosine series and a theoretical solution can be derived. The effectiveness of the proposed method is verified by a centrifuge test and an actual field measurement, which are extracted from previous investigations. Calculation results from the proposed method show good accord to centrifuge test results and field‐measured data. Meantime the predictions given by the proposed method are more accurate to estimate tunnel response compared with the previous method. Parametric analysis indicates that the volume loss ratio and the vertical clearance between two tunnels, are both significantly alleviate the tunnel response, but the effect of tunnel shear stiffness is slight. The theoretical solutions can be adopted to predict the overlying tunnel response induced by tunneling underneath in real projects.
Perfusion of hepatocellular carcinomas measured by diffusion‐derived vessel density biomarker: Higher hepatocellular carcinoma perfusion than earlier intravoxel incoherent motion reports
Authors: Li Xin‐Ming , Yao Dian‐Qi , Quan Xian‐Yue , Li Min , Chen Weibo , Wáng Yì Xiáng J. 
Published: February 2024

AbstractDiffusion‐derived vessel density (DVDD) is a physiological surrogate of the area of microvessels per unit tissue area. DDVD is calculated according to DDVD(b0b2) = Sb0/ROIarea0 – Sb2/ROIarea2, where Sb0 and Sb2 refer to the liver signal when b is 0 or 2 s/mm2. Pathohistological studies and contrast‐enhanced CT/MRI data showed higher blood volume in hepatocellular carcinoma (HCC) relative to native liver tissue. With intravoxel incoherent motion (IVIM) imaging, most authors paradoxically reported a decreased perfusion fraction of HCC relative to the adjacent liver. This study applied DDVD to assess the perfusion of HCC. MRI was performed with a 3.0‐T magnet. Diffusion‐weighted images with b‐values of 0 and 2 s/mm2 were acquired in 72 HCC patients. Thirty‐two patients had microvascular invasion (MVI(+)) and 40 patients did not have microvascular invasion (MVI(−)). Fifty‐eight patients had Edmondson–Steiner grade I or II HCC, and 14 patients had Edmondson–Steiner grade III or IV HCC. DDVD measurement was conducted on the axial slice that showed the largest HCC size. DDVD(b0b2) T/L = HCC DDVD(b0b2)/liver DDVD(b0b2). DDVD(b0b2) T/L median (95% confidence interval) of all HCCs was 2.942 (2.419–3.522), of MVI(−) HCCs was 2.699 (2.030–3.522), of MVI(+) HCCs was 2.988 (2.423–3.990), of Edmondson–Steiner grade I/II HCCs was 2.873 (2.277–3.465), and of Edmondson–Steiner grade III/IV HCCs was 3.403 (2.008–4.485). DDVD(b0b2) T/L approximately agrees with contrast agent dynamically enhanced CT/MRI literature data, whereas it differs from earlier IVIM study results, where HCC perfusion fraction was paradoxically lower relative to native liver tissue. A weak trend was noted with MIV(+) HCCs had a higher DDVD(b0b2) T/L than that of MVI(−) HCCs, and a weak trend was noted with the poorly differentiated group of HCCs (Edmondson–Steiner grade III and IV) had a higher DDVD(b0b2) T/L than that of the better differentiated group of HCCs (Edmondson–Steiner grade I and II).
The spatial landscape of T cells in the microenvironment of stage III lung adenocarcinoma
Authors: Zeng Ziqing , Du Weijiao , Yang Fan , Hui Zhenzhen , Wang Yunliang , Zhang Peng , Zhang Xiying , Yu Wenwen , Ren Xiubao , Wei Feng 
Published: February 2024

AbstractThis study aimed to provide more information for prognostic stratification for patients through an analysis of the T‐cell spatial landscape. It involved analyzing stained tissue sections of 80 patients with stage III lung adenocarcinoma (LUAD) using multiplex immunofluorescence and exploring the spatial landscape of T cells and their relationship with prognosis in the center of the tumor (CT) and invasive margin (IM). In this study, multivariate regression suggested that the relative clustering of CT CD4+ conventional T cell (Tconv) to inducible Treg (iTreg), natural regulatory T cell (nTreg) to Tconv, terminal CD8+ T cell (tCD8) to helper T cell (Th), and IM Treg to tCD8 and the relative dispersion of CT nTreg to iTreg, IM nTreg to nTreg were independent risk factors for DFS. Finally, we constructed a spatial immunological score named the GT score, which had stronger prognostic correlation than IMMUNOSCORE® based on CD3/CD8 cell densities. The spatial layout of T cells in the tumor microenvironment and the proposed GT score can reflect the prognosis of patients with stage III LUAD more effectively than T‐cell density. The exploration of the T‐cell spatial landscape may suggest potential cell–cell interactions and therapeutic targets and better guide clinical decision‐making. © 2024 The Pathological Society of Great Britain and Ireland.
The efficacy of iatrosedation and music listening techniques in attenuating dental anxiety in patients undergoing dental crown preparation: A randomized clinical trial
Authors: Lal Abhishek , Maqsood Afsheen , Ahmed Naseer , Altamash Sara , Al Rifaiy Mohammed Q. , Alsaif Rawan , Vohra Fahim , Mosaddad Seyed Ali , Heboyan Artak 
Published: February 2024

AbstractDental anxiety is a common problem encountered in dental clinics that affects both patients and dentists. Adequate management of dental anxiety is critical for optimal treatment outcomes for the patient. This study aims to assess the efficacy of two anxiety‐reduction techniques (iatrosedation and music listening) for dental crown preparation in adult patients. In this clinical trial, 60 patients were randomly assigned to three groups: Group 1, iatrosedation; Group 2, music listening; and Group 3, control. Patients in all three groups underwent dental crown preparation. To measure the anxiety levels of the patients, heart rate was calculated using a pulse oximeter, and verbal rating scale scores were assessed. One‐way analysis of variance, post hoc analysis, and Spearman's correlation were used to compare the mean values of the three groups. Significant differences were observed in the heart rate and verbal rating scale scores among individuals in the study groups. A more substantial reduction in anxiety levels was found in patients exposed to iatrosedation (Group 1), which was followed by music listening (Group 2). Recorded heart rate and verbal rating scores were the highest in the control group patients. The iatrosedation technique significantly reduced dental anxiety for patients undergoing dental crown treatment; however, music listening was less effective than iatrosedation. Educating patients regarding the dental care they are about to receive is vital for reducing their anxiety.
The unique role of meaning in life in the relationships between trait awe, subjective well‐being, and prosocial tendency: A network analysis
Authors: Lv Yichao , Xu Qian , Tang Qihui , Tao Yanqiang , Zhang Chao , Liu Xiangping 
Published: February 2024

AbstractAlthough individuals with higher trait awe (the tendency to experience awe) are known to be happier and more prosocial, there is limited understanding of the mechanisms underlying these complex relationships. This study uses network analysis to explore dimension‐level relationships between trait awe, meaning in life, subjective well‐being (SWB), and prosocial tendency in a joint network and to explore the bridging role of meaning in life in the network. A total of 538 adults (53.2% females; Mage = 19.86 ± 1.51) completed the survey. The network revealed unique and intricate connections between the dimensions of trait awe, meaning in life (i.e., the presence of and the search for meaning, abbreviated as POM and SFM), subjective happiness and life satisfaction (SWB), and prosocial tendency (i.e., willingness to donate money and volunteer time). Trait awe exhibited direct links to subjective happiness, life satisfaction, and prosocial tendency to donate money. Moreover, through POM and SFM, trait awe also exhibited indirect links to each dimension of SWB and prosocial tendency. Within the global network, POM was further identified as acting as a bridge node with the highest bridge strength and closeness, indicating that POM could efficiently transmit influences within the entire network. These findings highlight the distinct contributions of meaning in life to understanding the relationships between trait awe, SWB, and prosocial tendency, and provide valuable insights for improving SWB and fostering prosocial tendencies.
Validating the Enright Forgiveness Inventory in Ecuador
Authors: Fariña Francisca , Zhao Xiang , Novo Mercedes , Acurio Gabriela 
Published: February 2024

AbstractResearch on forgiveness is limited in Ecuador. This study validated the Enright Forgiveness Inventory–30 (EFI‐30) among 960 participants in Ecuador, resulting in robust reliability and validity values. Our findings provide avenues for future research and practices.
Achieving performance‐advantaged polydicyclopentadiene via sequential ring‐opening metathesis polymerization and radical reaction
Authors: Zhan Qingyan , Huangfu Fei , Yang Po 
Published: February 2024

AbstractHerein, we develop an approach to achieve performance‐advantaged polydicyclopentadiene (PDCPD) via sequential ring‐opening metathesis polymerization (ROMP) and radical reactions. We mix dicyclopentadiene (DCPD), Grubbs' catalyst and radical initiator in one pot, and then polymerize first at low temperature by ROMP and initiate radical reactions at elevated temperature. As a result of our strategy, the crosslink density of PDCPD increases, leading to outstanding performance. For instance, the glass transition temperature Tg of ROMP‐prepared PDCPD is ca 155–175 °C, whereas the Tg of PDCPD prepared by this strategy can be increased to 191 °C and can be further enhanced to 216 °C after polymerization at 250 °C. We believe this can help researchers design and synthesize high performance ROMP‐derived polymers and apply PDCPD in harsh conditions. © 2024 Society of Industrial Chemistry.
A randomized controlled trial of a distress screening, consultation, and targeted referral system for family caregivers in oncologic care
Authors: Applebaum Allison J. , Schofield Elizabeth , Kastrinos Amanda , Gebert Rebecca , Behrens Mia , Loschiavo Morgan , Shaffer Kelly M. , Levine Marcia , Dannaoui Aimee , Bellantoni Courtney , Miller Melissa F. , Zaleta Alexandra K. 
Published: February 2024

AbstractObjectiveDistress screening is standard practice among oncology patients, yet few routine distress screening programs exist for cancer caregivers. The objective of this study was to demonstrate the feasibility, acceptability, and preliminary efficacy of Cancer Support Source‐CaregiverTM (CSS‐CG, 33‐item), an electronic distress screening and automated referral program with a consultation (S + C) to improve caregiver unmet needs, quality of life, anxiety, depression, and distress relative to Enhanced Usual Care (EUC; access to educational materials).Method150 caregivers of patients with varying sites/stages of cancer were randomized to S + C or EUC and completed assessments at baseline, 3‐months post‐baseline, and 6‐months post‐baseline. A subset of participants (n = 10) completed in‐depth qualitative interviews.ResultsS + C was feasible: among 75 caregivers randomized to S + C, 66 (88%) completed CSS‐CG and consultation. Top concerns reported were: (1) patient's pain and/or physical discomfort; (2) patient's cancer progressing/recurring; and (3) feeling nervous or afraid.Differences between groups in improvements on outcomes by T2 and T3 were modest (ds < 0.53) in favor of S + C. Qualitative data underscored the helpfulness of S + C in connecting caregivers to support and helping them feel cared for and integrated into cancer care.ConclusionsS + C is feasible, acceptable, and yields more positive impact on emotional well‐being than usual care. Future studies will examine programmatic impact among caregivers experiencing higher acuity of needs, and benefits of earlier integration of S + C on caregiver, patient, and healthcare system outcomes.
Integrative analysis of PTEN‐related hub genes and validating drug targets for colorectal cancer
Authors: Wang Gang , Zhu Zhi Min , Wang Kun 
Published: February 2024

AbstractColorectal cancer (CRC) is a heterogeneous disease and one of the most prevalent malignancies worldwide. Previous research has indicated that phosphatase and tensin homolog (PTEN)‐related genes found in CRC may serve as potential biomarkers for individualized treatment options. The present study aimed to examine the association between PTEN‐related genes and the prognosis of CRC patients by evaluating the significance of PTEN‐related hub genes and determining potential mechanisms and genes associated with them. Gene expression profiles and clinical information were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. At present, PTEN mutations have been identified in 7% of CRC patients, according to the most recent TCGA data. Differential expression analysis revealed 54 genes as differentially expressed genes (DEGs) between PTEN‐related genes and GEO databases (GSE39582 and GSE6263). Further gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted on PTEN‐related DEGs. The prognostic efficacy of the PTEN‐related DEG signature was assessed using Kaplan–Meier survival and receiver operating characteristic curve analyses. Bioinformatics methods were utilized to analyze the correlation between PTEN‐related DEGs and CRC prognosis, survival, and drug efficacy. Through these analyses, eight prognostic‐related PTEN‐related hub genes (PPARGC1A, NTRK2, ANK2, PLCB4, STC2, PLAU, CDKN1A, and HPGDS) were identified and a risk prognosis model was constructed. Notably, NTRK2 and HPGDS were found to affect drug treatment response in CRC. Targeting these prognostic‐related PTEN‐related hub genes can regulate cell death signaling, which may benefit the prognosis of CRC patients and improve drug sensitivity.