bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Experience and Influence of Youth in China in Coping with Stressful Life Events during the Post–COVID-19 Pandemic Era: A Qualitative Study
Authors: Guo Yannan , Xu Ziruo , Ding Hexiao , Wang Cailing , Peng Xinran , An Yuping , Wang Jing , Hu Wangjing , Li Sicheng , Hui Zhengyang , Xia Wei 
Published: November 2023

Abstract
Background:
The COVID-19 pandemic has had a profound effect on the mental well-being of individuals, particularly among the youth. The experience of stressful life events has been linked to increased poor mental health. While the immediate effects have been documented, there is evidence to suggest that the pandemic’s impact on mental health could persist in the long run. Additionally, the pandemic has influenced coping strategies and forms of social support.
Methods:
A phenomenological descriptive study was conducted to elucidate the changes in strategies adopted by youth to cope with stressful life events during the COVID-19 era. Thirty-three Chinese youths aged 15 to 24 years who have experienced regular COVID-19 pandemic prevention and control were invited to complete a semi-structured interview. Colaizzi’s method was used to analyze the data. Triangulation was adopted to ensure the validity.
Results:
Four key themes were generated, including (1) negative changes in mental health, (2) positive changes in mental health, (3) changes in the adoption of coping strategies, and (4) the utilization of internet-based coping strategies.
Conclusions:
The results could guide the development of interventions to help the youth cope with stressful life events and further promote mental health among this population.
Trial registration:
This study has prospectively been registered on the Chinese Clinical Trial Registry (ID: ChiCTR2200060894) on 14/06/2022.
WITHDRAWN: Molecular and cytogenetic analyses in Geranium macrorrhizum L. wild Italian plants
Authors: Cardinali Irene , Ceccarelli Marilena 
Published: November 2023

Abstract
Geranium macrorrhizum L. is a herbaceous species native to southern Europe, and introduced in central Europe and North America. It is widely distributed also in Italy, up to Campania region as the southern limit. In this study, molecular and cytogenetic analyses were carried out on 22 wild plants, collected in central and southern Italy, compared to five cultivated plants, with the main purpose to identify those living near the Marmore waterfalls in central Italy, recently described as the new species G. lucarinii. Four barcoding markers (rbcL, matK, trnH-psbA intergenic spacer, and internal transcribed spacer region, ITS) were sequenced and their variability among the plants was evaluated. Chromosome numbers were determined and 45S rDNA was physically mapped by fluorescence in situ hybridization (FISH) in both wild and cultivated plants. Moreover, genomic affinity between wild and cultivated plants was evaluated by genomic in situ hybridization (GISH). Our results confirmed that all the plants, including Marmore population, belong to G. macrorrhizum. Barcoding analyses showed a close similarity among the wild plants, as well as a differentiation, although not significant, between the wild plants on one hand and the cultivated plants on the other. Integrated studies focusing on morphological, genetic and ecological characterization of a larger number of wild populations will allow for knowing the extent of the variability within the species.
Ameliorative Effects of Alchornea cordifolia Extract on Bisphenol A-Induced Obesity in Wistar rats
Authors: Agboola Abdulhakeem Rotimi , Itam Ako Hogan , Ekeleme Chinedum Martins , Agwupuye Eyuwa Ignatius , Ahmed Zuwairatu Oyiza , Igiakong Godswill Peter , Agbor Gideon Bassey , Abubakar Asmau Niwoye , Adamu Zainab , David-Oku Esien 
Published: November 2023

Abstract

Background
Bisphenol A (BPA) is a chemical found in plastic bottles and has been linked to obesity and its associated health problems. Due to the cost and possible side effects that are associated with the orthodox medications employed for weight management, there is a shift in attention to local medicinal plants as they are readily available and are believed to be free from negative side effects. One plant that has found application for weight loss amongst the local population in Nigeria is Alchornea cordifolia which commonly grows in Africa and has been confirmed to possess numerous medicinal properties which include; anti-inflammatory, anti-diarrheal, hepaprotective, antiviral, and anti-diabetic effects.
Objective:
To investigate the effects of Alchornea cordifolia ethanol extract (ACEA) against the harmful impacts caused by BPA-induced obesity in Wistar rats.
Methods
ACEA was prepared and tested on BPA-induced Wistar rats. Thirty (30) young male Wistar rats were divided into five groups with six rats per group where Group 1 (Normal control) received commercial feed and water only, Group 2 (BPA) received commercial feed and 50 mg/kg of BPA, Group 3 (BPA + Orlistat (30 mg/kg), Group 4 (BPA + A.cordifolia (500 mg/kg)) ethanol extract of A.cordifolia, Group 5 (BPA + A.cordifolia(1000 mg/kg) ethanol extract of A.cordifolia. The parameters studied after obesity was induced and after treatments are; Anthropometric parameters; body weight, waist circumference, lees index and biochemical and kidney function parameters; fasting blood glucose, serum glucose, total protein, relative weight of the kidneys, serum levels of urea, creatinine, uric acid, concentrations of sodium, chloride, and potassium, serum levels of total cholesterol (TC), triacylglycerides (TAG), high-density lipoprotein cholesterol (HDL-c) lipase, low-density lipoprotein cholesterol (LDL-c) and HMG-CoA reductase activity.
Results
Results showed that Bisphenol-A caused obesity and related complications after 4 weeks of administration as evidenced by the elevation of the anthropometric, biochemical and kidney function parameters. However, treatment with low (500 mg/kg) and high dose (1000 mg/kg) of A.cordifolia reduced the elevated levels of the anthropometric, biochemical and kidney function parameters in the Wistar rats.
Conclusion
The results obtained from this study demonstrates the potential of ACEA in the management of obesity and its related complications
Molecular evaluation of SARS-CoV-2 in post-vaccinated patients of Malakand division Khyber Pakhtunkhwa
Authors: abbas waseem , Amir Syed , Rafiullah  
Published: November 2023

Abstract

Coronavirus disease (COVID-19) is one of the epidemic diseases caused by the SARS-CoV-2 virus. The priority of the health community is the development of various reliable vaccinations to combat the COVID-19 pandemic. Some vaccines have been developed to combat the Covid-19 pandemic and humans have been allowed to use. However, vaccinated people have had frequent infections in many areas. The purpose of this study is to find out the efficacy of the SARS-CoV-2 vaccine in Malakand Division. A total of 500 samples were collected from infected subjects through nasopharyngeal swabs. The samples were then categorized based on vaccination type and efficacy. The RNA was extracted and subjected to RT-PCR for detection SARS-CoV-2. Out of 500 vaccinated individuals, 173 were positive. The rate of SARS-CoV-2 recurrent infections was high among all subjects. The most effective vaccines were Pfizer BioNTech with an efficiency 85%. The remaining vaccines such as Moderna showed an efficiency of 75%, Sputnik-V vaccine (73%), AstraZeneca (72%), CoronaVac-SinoVac (60%), Sinopharm BBIBP (55%) and Cansino Vaccine (50%). The study concludes that Pfizer BioNTech is the most effective vaccine among all the tested vaccines and can be recommended to minimize the risk of recurrent infection.
Metabolic Engineering of Escherichia coli for the Production of 5-Aminolevulinic Acid Based on Combined Metabolic Pathway Modification and Reporter-Guided Mutant Selection (RGMS)
Authors: Yang Yuting , Zou Yuhong , Chen Xi , Sun Haidong , Hua Xia , Johnston Lee , Zeng Xiangfang , Qiao Shiyan , Ye Changchuan 
Published: November 2023

Abstract

Background
5-Aminolevulinic acid (ALA) recently received much attention due to its potential application in many fields such as medicine, nutrition and agriculture. Metabolic engineering is an efficient strategy to improve microbial production of 5-ALA.
Results
In this study, an ALA production strain of Escherichia coli was constructed by rational metabolic engineering and stepwise improvement. A metabolic strategy to produce ALA directly from glucose in this recombinant E. coli via both C4 and C5 pathways was applied herein. The expression of a modified hemARS gene and rational metabolic engineering by gene knockouts significantly improved ALA production from 765.9 to 2056.1 mg/L. Next, we tried to improve ALA production by RGMS-directed evolution of eamA gene. After RGMS, the ALA yield of strain A2-ASK reached 2,471.3 mg/L in flask. Then we aimed to improve the oxidation resistance of cells by overexpressing sodB and katE genes and ALA yield reached 2703.8 mg/L. A final attempt is to replace the promoter of hemB gene in genome to inhibit the hemB gene. After 24 h cultivation, a high ALA yield of 19.02 g/L was achieved by 108-ASK in a 5 L fermenter.
Conclusions
These results suggested that an industrially competitive strain can be efficiently developed by metabolic engineering based on combined rational modification and optimization of gene expression.
Protein and transcript expression levels of TRIP13 signify worse prognosis in endometrioid carcinoma
Authors: Wu Quan , Wang Zitong , He Cai , Guo Jingwei , Xu Chengcheng , Gao Yehan , Zhang Yichen , Ma Jiajie , Wang Zhe 
Published: November 2023

Abstract

This study investigated the genes implicated in endometrioid adenocarcinoma development to provide insights into the molecular diagnosis, targeted therapy, and prognostic prediction of the disease. Two mRNA microarray datasets were obtained from the Gene Expression Omnibus. Differentially expressed genes were identified, and a protein–protein interaction network was constructed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed on the genes within the network modules. Prognostic significance was determined through a survival analysis to identify the key genes. The UALCAN website was used to validate TRIP13 mRNA expression, and immunohistochemical staining was performed to evaluate TRIP13 protein expression in patient samples. Sixteen genes were significantly associated with patient prognosis. The results revealed significantly elevated TRIP13 transcription and protein expression levels in endometrial cancer tissues compared to those in normal tissues. High TRIP13 expression was significantly correlated with shorter survival and considered an independent risk factor for endometrioid adenocarcinoma. Further, a negative correlation between TRIP13 expression and infiltrating CD8 + T cell levels in uterine corpus endometrial carcinoma was observed. This study validated the correlation between TRIP13 expression and poor patient prognosis, suggesting that TRIP13 may be a biomarker for endometrioid adenocarcinoma prognosis predictions.
Properties of layer V pyramidal neurons in the primary motor cortex representing acquired motor skills
Authors: Kida Hiroyuki , Sakimoto Yuya , Mitsushima Dai 
Published: November 2023

Abstract
Layer V neurons in primary motor cortex (M1) are required for motor skill learning. We analyzed training-induced plasticity using a whole-cell slice patch-clamp technique with a rotor rod task, and found that training induces diverse changes in intrinsic properties and synaptic plasticity in M1 layer V neurons. Although the causal relationship between specific cellular changes and motor performance is unclear, by linking individual motor performance to cellular/synaptic functions, we identified several cellular and synaptic parameters that represent acquired motor skills. With respect to cellular properties, motor performance was positively correlated with resting membrane potential and fast afterhyperpolarization, but not with the membrane resistance, capacitance, or threshold. With respect to synaptic function, the performance was positively correlated with AMPA receptor-mediated postsynaptic currents, but not with GABAA receptor-mediated postsynaptic currents. With respect to live imaging analysis in Thy1-YFP mice, we further demonstrated a cross-correlation between motor performance, spine head volume, and self-entropy per spine. In the present study, among the diverse changes that occur in M1 layer V pyramidal neurons after motor training, we identified several changes that represent acquired motor skills. These results suggest that systematic control of specific intracellular parameters and enhancement of synaptic plasticity in M1 layer V neurons may be useful for improving motor skills.
The Cycles of Decrees: Evidence From the Italian Parliament 1
Authors: Lagona Francesco , Mingione Marco , Padovano Fabio 
Published: November 2023

Abstract

This research provides an empirical test of the predictions of the Political Legislation Cycle theory about decrees using disaggregated data made recently available by official databanks of the Italian Republic. The analysis focuses on the five different types of decrees for which long enough data series are available: law-decrees (decreti legge), legislative decrees (decreti legislativi), DPR (decrees of the President of the Republic), implementing decrees (decreti attuativi) and independent decrees (decreti indipendenti). Multiple sources of latent heterogeneity affect the production of decrees due to multilevel factors that operate jointly with observed covariates at all levels of the hierarchy of the data. We account for these factors by estimating a multilevel negative binomial regression model with random effects on the mean for repeated measurements of disaggregated counts of decrees, implemented under multiple Italian governments, nested within the first 17 legislatures of the Italian Republic. The estimates of the model broadly confirm the theory’s prediction that decrees tend to be approved in the earlier stages of the legislature; this prediction receives stronger support the more the institutional characteristics of each type of decree resemble those of the theoretical variable. Finally, the analysis confirms the prediction of the PLC theory about the legislators’ choice of the legislative instruments through which they satisfy the conflicting interests of special interest groups and voters, shedding light on the timing of the activities of lobbies.
JEL classification codes: D72, C49, H61, H62
Unlocking Corrosion Secrets: A Comprehensive Analysis of Acoustic Emission Data Using Continuous Wavelet Transform and Deep Learning for Steel Pipeline Monitoring
Authors: Hassan Farrukh , Rahim Lukman AB , Khan Muhammad Irfan , Adnan Muhamed Hariz Mohamed , Ali Shahid , Qayyum Faiza 
Published: November 2023

Abstract
The analysis of acoustic emission data in the time and frequency domains can provide useful insights about the state of steel pipelines, although research in this field is limited. The research shortage has been triggered due to specific problems provided by elements such as irregular structure geometries, material dependencies, and insufficient training data. To address the lack of training data, we created a new dataset of scalogram images for deep learning-based classification approaches. Monitoring a 500-meter-long steel oil and gas pipeline provided experimental data. The acoustic emission waveforms were preprocessed, tagged, and classified based on three critical characteristics: AE-mean, kurtosis, and amplitude. The continuous wavelet transform was used to translate these waveform instances into the time-frequency domain. To. The abstract should be an objective representation of the article and it must not contain results that are not presented and substantiated in the main text and should not exaggerate the main conclusions. State-of-the-art deep convolutional neural networks were used as benchmarks to assess the effectiveness of the proposed dataset. Surprisingly, the unique dataset achieved a classification accuracy of 91.0%. Furthermore, the impact of initial learning rate and L2 regularization hyperparameters were evaluated for EfficientNet-b0. The availability of this dataset opens the door to a plethora of corrosion detection applications, providing substantial prospects in this field.
Black holes regulate cold gas accretion in massive galaxies
Authors: Wang Tao , Xu Ke , Wu Yuxuan , Shi Yong , Elbaz David , Ho Luis , Zhang Zhi-Yu , Gu Qiusheng , Wang Yijun , Shu Chenggang , Yuan Feng , Xia Xiaoyang , Wang Kai 
Published: November 2023

Abstract

Nearly every massive galaxy contains a supermassive black hole (BH) at its center. For decades, both theory and numerical simulations have indicated that BHs play a central role in regulating the growth and quenching of galaxies. Specifically, BH feedback by heating or blowing out the interstellar medium (ISM) serves as the groundwork for current models of massive galaxy formation. However, direct evidence for such an impact on the galaxy-wide ISM from BHs has only been found in some extreme objects. For general galaxy populations, it remains unclear whether and how BHs impact the ISM. Here based on a large sample of nearby galaxies with measurements of masses of both black holes and atomic hydrogen, the major component of cold ISM, we reveal that the atomic hydrogen content ( fHI = MHI/M*) is tightly and anti-correlated with black hole mass (M_BH) with f_HI ~M_BH^(-α) (α ∼ 0.5 − 0.6). This correlation is valid across five orders of magnitude in M_BH. Once this correlation is taken into account, fHI loses dependence on other galactic parameters, demonstrating that M_BH serves as the primary driver of fHI. These findings provide critical evidence for how the accumulated energy from BH accretion impacts galaxy-wide ISM, representing a crucial step forward in our understanding on the role of BHs in regulating the growth and quenching of massive galaxies.
Design, Formulation, Evaluation and Statistical Optimization of Coated Floating Pulsatile Tablets for Modified Chronopharmacotherapy of Rheumatoid Arthritis
Authors: jyothirmayi pasam 
Published: November 2023

Abstract
Objective
The main aim of designing Coated Floating Pulsatile Tablets (CFPT) of Tramadol hcl for statistical optimization and to perform in vitro and in vivo buoyancy evaluation for modified chronopharmacotherapy of rheumatoid arthritis.
Methods
The formulation was designed with the immediate core covered with a compression coat followed by the upper buoyant layer. Best formulations of Immediate burst release core tablets were compression coated with polymer HPMC K15M, Ispaghula gum (IG), and Tamarindus gum (TG) to assign suitable lag time to formulation. HPMC K100M, Ispaghula gum, and their combination were compressed as the buoyant layers. Parameters such as BLT, TFT, %SI,% CDR were Statistical optimized by surface and contour plots AND invivo buoyancy was also done.
Results
Immediate release core tablets were optimized with formulation IF2 which contains sodium starch Glycolate 8% as immediate release agent, and then in compression coated buoyant formulation CFPT was optimized by DoE optimization showing BF4 and BF8 exhibiting good floating behavior and lag time, drug release for a period of 11 hrs. Abdominal X-ray imaging of rabbits after oral administration of the tablets, confirmed the floating behavior and lag time.
Conclusion
As the formulation showed delay in drug release both in in vitro and showed in vivo buoyancy, hence nighttime administration could be beneficial to reduce the rheumatoid arthritis complications in the early morning.
Relevance of pathognomonic changes in multiple sclerosis patients in remission for oral and maxillofacial surgery– a pilot study of case series
Authors: Fehlhofer Jakob , Ries Jutta , Nickel Florian Tobias , Rothhammer Veit , Schwab Stefan , Kesting Marco , Buchbender Mayte 
Published: November 2023

Abstract
Objectives
Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease of unknown etiology that affects the central nervous system and can lead to neurological impairment. Our aim was to determine whether MS patients also show inflammatory changes in the oral cavity more frequently than healthy individuals.
Material and Methods
We examined plaque samples for various mediators and their correlation with clinical findings. A study group (MS) and a control group were examined and compared. The plaque samples were analyzed for the expression of interleukins (IL-2, -6, -10), matrix metalloproteinases (MMP-7, MMP-9) and a surface antigen CD90 by quantitative real-time PCR. Clinical parameters examined were the Mombelli plaque index (MPI), bleeding on probing (BOP) index, gingival pocket depth (PD) and Decayed, missing and filled tooth (DMFT) index.
Results
The expression of MMP9 was significantly (p = 0.035) higher in the control group. The expression of IL-2 was increased 4-fold in the MS group, but this difference was not statistically significant. The mean PD (p < 0.001) and BOP index (p = 0.029) were increased in the study group.
Conclusion
The clinical parameters of the BOP index and PD were significantly amplified in the MS patients. However, no causal relationship could be established in this case series, thus for the oral and maxillofacial surgical treatment no evidence for restrictions could be derived.
Clinical Relevance
With the worldwide increasing incidence of MS, it’s important to obtain insights into the effects of the disease on the oral cavity. The study was registered in the German clinical trial registry (DRKS00022956, registered 28/08/2020).