bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional integration of natural killer cells in a microfluidically perfused liver on-a-chip model
Authors: Fahrner René , Gröger Marko , Settmacher Utz , Mosig Alexander S. 
Published: October 2023

Abstract
Objective
The liver acts as an innate immunity-dominant organ and natural killer (NK) cells, are the main lymphocyte population in the human liver. NK cells are in close interaction with other immune cells, acting as the first line of defense against pathogens, infections, and injury.
A previously developed, three-dimensional, perfused liver-on-a-chip comprised of human cells was used to integrate NK cells, representing pivotal immune cells during liver injury and regeneration. The objective of this study was to integrate functional NK cells in an in vitro model of the human liver and assess utilization of the model for NK cell-dependent studies of liver inflammation.

Results
NK cells from human blood and liver specimen were isolated by Percoll separation with subsequent magnetic cell separation (MACS), yielding highly purified blood and liver derived NK cells. After stimulation with toll-like-receptor (TLR) agonists (lipopolysaccharides, Pam3CSK4), isolated NK cells showed increased interferon (IFN)-gamma secretion. To study the role of NK cells in a complex hepatic environment, these cells were integrated in the vascular compartment of a microfluidically supported liver-on-a-chip model in close interaction with endothelial and resident macrophages. Successful, functional integration of NK cells was verified by immunofluorescence staining (NKp46), flow cytometry analysis and TLR agonist-dependent secretion of interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha. Lastly, we observed that inflammatory activation of NK cells in the liver-on-a-chip led to a loss of vascular barrier integrity. Overall, our data shows the first successful, functional integration of NK cells in a liver-on-a-chip model that can be utilized to investigate NK cell-dependent effects on liver inflammation in vitro.
Late diagnosis of respiratory syncytial virus and influenza co-infection during coronavirus disease 2019 pandemic: a case report
Authors: Barahimi Elham , Azad Mehdi Hassani , Hesarooeyeh Zahra Ghaeini , Hafshejani Niloofar Hosseini , Defaee Sahar , Seddighi Narjes 
Published: October 2023

Abstract
Background
Respiratory syncytial virus (RSV)-induced disease is one of the important causes of flu-like illness in older adults and can cause serious disease in those who are at high-risk medical conditions. During coronavirus disease 2019 (COVID-19) pandemic, because of overlapping symptoms of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) infection with other respiratory infections, diagnosing diseases based on clinical and radiological findings was challenging and could cause misdiagnosis.

Case presentation
An 87-year-old Persian man was admitted to the hospital due to loss of consciousness, respiratory distress, tachypnea, and oliguria. He had previously hospitalized because of cough, fever, loss of appetite, and fatigue. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) polymerase chain reaction (PCR) test was performed which was negative; however, based on ground glass opacity on his chest computed tomography (CT) scan and being on the outbreak of COVID-19, he fulfilled case definition of COVID-19; therefore, he received protocol’s treatment (remdesivir) for COVID-19 and relatively recovered and discharged. In our center, we requested brain and chest CT scans, blood tests, and multiplex PCR. Multiplex PCR revealed co-infection of influenza virus and RSV. Although we had started pneumonia and sepsis treatment, old age, weak immune system and the delay in initiation of right antibiotic and antivirus therapy altogether led him to die.

Conclusion
As a takeaway lesson of this case report, it is necessary to pay attention to viruses that show similar symptoms during future specific virus pandemics, especially in patients with old age and weak immune systems.
Vocal fold restoration after scarring: biocompatibility and efficacy of an MSC-based bioequivalent
Authors: Svistushkin Mikhail , Shpichka Anastasia , Bikmulina Polina , Fayzullin Alexey , Zolotova Anna , Kosheleva Nastasia , Selezneva Liliya , Shavkuta Boris , Lobacheva Viktoria , Nikiforova Anna , Kochetkov Peter , Kotova Svetlana , Starostina Svetlana , Shekhter Anatoly , Svistunov Andrey , Svistushkin Valeriy , Timashev Peter 
Published: October 2023

Abstract
Background
There is growing interest to application of regenerative medicine approaches in otorhinolaryngological practice, especially in the framework of the therapy of vocal fold (VF) scar lesions. The used conservative and surgical methods, despite the achieved positive outcomes, are frequently unpredictable and do not result in the restoration of the VF’s lamina propria’s structure, which provides the mechanical properties necessary for vibration. In this connection, the aim of this study was to ascertain the safety and efficacy of a bioequivalent in the treatment of VF scars using a rabbit model of chronic damage.

Methods
The bioequivalent consisted of a hydrogel system based on a PEG-fibrin conjugate and human bone marrow-derived MSC. It was characterized and implanted heterotopically into rats and orthotopically into rabbits after VF scar excision.

Results
We showed that the fabricated bioequivalent consisted of viable cells retaining their metabolic and proliferative activity. While being implanted heterotopically, it had induced the low inflammatory reaction in 7 days and was well tolerated. The orthotopic implantation showed that the gel application was characterized by a lower hemorrhage intensity (p = 0.03945). The intensity of stridor and respiratory rate between the groups in total and between separate groups had no statistically significant difference (p = 0.96 and p = 1; p = 0.9593 and p = 0.97…1, respectively). In 3 days post-implantation, MSC were detected only in the tissues closely surrounding the VF defect. The bioequivalent injection caused that the scar collagen fibers were packed looser and more frequently mutually parallel that is inherent in the native tissue (p = 0.018). In all experimental groups, the fibrous tissue’s ingrowth in the adjacent exterior muscle tissue was observed; however, in Group 4 (PEG-Fibrin + MSC), it was much less pronounced than it was in Group 1 (normal saline) (p = 0.008). The difference between the thicknesses of the lamina propria in the control group and in Group 4 was not revealed to be statistically significant (p = 0.995). The Young’s modulus of the VF after the bioequivalent implantation (1.15 ± 0.25 kPa) did not statistically significantly differ from the intact VF modulus (1.17 ± 0.45 kPa); therefore, the tissue properties in this group more closely resembled the intact VF.

Conclusions
The developed bioequivalent showed to be biocompatible and highly efficient in the restoration of VF’s tissue.
Enhancing a Sphaerobacter thermophilus ω-transaminase for kinetic resolution of β- and γ-amino acids
Authors: Wegner Uwe , Matthes Falko , von Wirén Nicolaus , Lemke Ina , Bode Rüdiger , Vorbrodt H.-Matthias , Rauter Marion , Kunze Gotthard 
Published: October 2023

AbstractSphaerobacter thermophilus synthesizes an ω-transaminase (ω-TA) that allows the production of enantiomerically pure β-amino acids. To obtain ω-TA variants with a higher activity and more favorable properties for industrial use, we modified critical amino acid residues either in the catalytic center or in a previously proposed signature motif critical for aromatic β-amino acid ω-TAs. Seventeen different variants of this enzyme were generated and their activity was examined with four β-amino acids and one γ-amino acid, and compared with the wildtype’s activity. Among all variants, seven showed up to ninefold higher activity with at least one of the tested substrates. For most of these seven variants, the temperature optimum was even lower as in the wild type enzyme, with keeping a high temperature stability, making them more valuable for industrial purposes. Our results indicate that for the production of enantiomerically pure β-amino acids replacement of critical amino acid residues in the proposed signature motif of ω-TAs is a more effective strategy than modifying their catalytic center. Another finding was, that the proposed motif is not only suitable for aromatic amino acid ω-TAs, because some of the variants have a higher activity with β-alanine or β-leucine than with aromatic β-amino acids.
Graphical Abstract
Joint channel and impulse noise estimation based on compressed sensing and Kalman filter for OFDM system
Authors: Zhao Yiting , Li Youming , Shi Shoudong , Yu Jianding 
Published: October 2023

AbstractImpulse noise (IN) widely exists in many communication systems, which seriously affects the performance of OFDM communication systems. A joint channel and IN estimation method based on all subcarriers is designed. This method uses a sparse Bayesian learning (SBL) algorithm incorporating forward–backward Kalman filter (FB-Kalman) to tackle the problem of joint channel and IN estimation and data detection for OFDM systems. Firstly, the channel impulse response and IN are regarded as unknown sparse vectors, and a SBL framework using all subcarriers is proposed to estimate the unknown vector. The SBL theory is used based on the prior distribution of variables, and then the forward–backward joint system is established, which applies the data detection simultaneously. We also propose the FB-Kalman implementation algorithm by using the expectation maximization updates. Explicit expressions of mean and covariance matrix of the posterior distribution are derived in the E-step. Simulation results show that the proposed algorithm improves the normalized mean square error and bit error rate performance of OFDM system in the presence of IN communication environment.
Vulnerability to health and well-being of internally displaced persons (IDPs) in Myanmar post-military coup and COVID-19
Authors: Khai Tual Sawn 
Published: October 2023

AbstractAcross the globe, the COVID-19 pandemic has aggravated particular challenges for internally displaced people (IDPs). Over 1.9 million people in Myanmar have been displaced due to the escalation of armed conflict after the military coup in 2021. The vulnerability faced by IDPs in Myanmar, coupled with the impact of the recent military coup and the ongoing COVID-19 pandemic, has received little global attention. This study examined how military coup exacerbated the health and well-being of IDPs in Myanmar post the military coup. The study employed purposive sampling and Non-Government Organisations (NGOs) referrals to find participants. Qualitative in-depth telephone interviews were conducted with a total of 17 IDPs. A thematic analysis of the findings indicates that IDPs experience anxiety and fear daily, adversely affecting their mental health due to the increased escalation of armed conflict and attacks on civilian places, including IDPs shelters. Some IDPs contract COVID-19 and suffer from malaria and dengue fever owing to their precarious living conditions. Moreover, the military’s restrictions on humanitarian aid distribution, including healthcare, medicine, and food, have severely impacted the health and well-being of IDPs in Myanmar, exacerbating food shortages and limiting healthcare access.
Effectiveness and feasibility of a motivational interviewing intake (MII) intervention for increasing client engagement in outpatient addiction treatment: an effectiveness-implementation hybrid design protocol
Authors: Hurlocker Margo C. , Moyers Theresa B. , Hatch Melissa , Curran Geoffrey , McCrady Barbara , Venner Kamilla L. , Witkiewitz Katie 
Published: October 2023

Abstract
Background
Client discontinuation from outpatient addiction treatment programs is common, and the initial intake is the service delivery point with the highest attrition rate. Replacing the comprehensive intake assessment with a person-centered Motivational Interviewing (MI) intervention is a potential solution to address provider and client concerns about the disengaging, time-intensive nature of the typical initial intake. It remains unclear whether the use of an alternative to the standard intake at the initial visit can fit within typical organizational reporting requirements, whether it decreases attrition, and whether implementation of person-centered intake procedures within outpatient addiction treatment programs is feasible, acceptable, and can be sustained.

Purpose
To describe the methods and design of an effectiveness-implementation hybrid type 1 trial of a Motivational Interviewing at Intake (MII) intervention using the Consolidated Framework for Implementation Research (CFIR).

Methods
The study will determine the effectiveness of two intake conditions: (1) standard comprehensive intake assessment (intake-as-usual [IAU]), and (2) MII consisting of a person-centered discussion between provider and client about the client’s desire and intent to enter treatment. Although both interventions are focused on understanding client presenting complaints and needs for treatment, the delivery differs as the IAU uses a semi-structured assessment guide, while MII applies the theory of MI to have a conversation about treatment engagement. Adults seeking outpatient addiction treatment services will be randomly assigned to the MII condition (n = 75) or the IAU condition (n = 75). Primary outcomes will be client engagement (i.e., treatment entry, attendance, and completion) obtained from the electronic medical record. Secondary outcomes (client motivation and therapeutic alliance) will be putative mechanisms of client engagement assessed immediately before and after the intake. The trial also will explore determinants of effective, sustainable implementation using assessments of organizational readiness and capacity to change, as well as interviews on MII implementation feasibility.

Conclusion
This trial of an MII intervention will investigate the feasibility of a motivational intervention as an initial contact with substance use treatment-seeking clients as well as indicators of intervention effectiveness within the systems where it is employed.
Trial registration Clinicaltrials.gov identifier: NCT05489068
Nano-biomaterials and advanced fabrication techniques for engineering skeletal muscle tissue constructs in regenerative medicine
Authors: Han Seokgyu , Cruz Sebastián Herrera , Park Sungsu , Shin Su Ryon 
Published: October 2023

AbstractEngineered three-dimensional (3D) tissue constructs have emerged as a promising solution for regenerating damaged muscle tissue resulting from traumatic or surgical events. 3D architecture and function of the muscle tissue constructs can be customized by selecting types of biomaterials and cells that can be engineered with desired shapes and sizes through various nano- and micro-fabrication techniques. Despite significant progress in this field, further research is needed to improve, in terms of biomaterials properties and fabrication techniques, the resemblance of function and complex architecture of engineered constructs to native muscle tissues, potentially enhancing muscle tissue regeneration and restoring muscle function. In this review, we discuss the latest trends in using nano-biomaterials and advanced nano-/micro-fabrication techniques for creating 3D muscle tissue constructs and their regeneration ability. Current challenges and potential solutions are highlighted, and we discuss the implications and opportunities of a future perspective in the field, including the possibility for creating personalized and biomanufacturable platforms.
Dairy consumption in adults in China: a systematic review
Authors: Yang Shuhua , Bhargava Nupur , O’Connor Aileen , Gibney Eileen R. , Feeney Emma L. 
Published: October 2023

Abstract
Background
Research on dairy consumption in China is lacking, however, some evidence has demonstrated significant changes in recent years, with a reported increase in the overall consumption of dairy products. To fully understand these changes, a systematic review was conducted to examine reported dairy intakes and differences between dairy consumption in different population groups in China. Methods: Web of Science, Embase, and PubMed databases were searched for studies published from January 2000 to September 2022. The China National Knowledge Infrastructure (CNKI) was used to retrieve papers available in Chinese. Papers reporting dietary intakes of dairy consumption across age, sex, and geographical location sub-groups were considered for inclusion in this review. In addition, this review includes the consumption of different types of dairy foods and changes in dairy intake over time. Results: Forty-seven papers were included in the present study. Twelve papers examined dairy consumption across age groups, showing that middle-aged adults tend to consume less dairy than other age groups. Studies comparing across location-specific cohorts reported dairy intakes among urban populations were higher than rural, as well as being higher than the national average. Coastal, Northern and Eastern residents consumed more dairy products than those living in other regions of China, and people in larger cities had higher reported intakes than smaller cities. Milk was the primary dairy product reportedly consumed by Chinese population, followed by yogurt. Concerning sex, evidence showed that females generally reported a greater daily dairy intake than males. Conclusions: This review shows that, in China, several different population groups displayed significant differences in the amount and type of dairy consumed. When considering the incorporation of dairy products into healthy eating guidelines or positioning specific dairy products on the market, it is important to consider the differences and variations in consumption patterns within population groups.
Bank efficiency in Middle East and North African countries: Does political connection type matter?
Authors: Lassoued Naima , Khanchel Imen , Fakhfakh Imen 
Published: October 2023

AbstractThis study examines the effects of the political connections of chief executive officers (CEOs) or directors on technical, allocative, and cost bank efficiencies examining a panel of 144 banks operating in 12 Middle Eastern and North African (MENA) countries observed over the 2008–2021 period. Using random effect tobit regressions, we find that the three types of political connections explored (aggregate, CEO, and board of directors) have negative effects on banks’ technical and cost efficiencies. In addition, CEO political connections exhibit superior explanatory power. These findings remain robust when we consider the sample in terms of monarchist and republican countries. Further evidence reveals that the effect of political connections is observed more strongly during the pandemic period (2020–2021) than during the 2008–2009 financial crisis period. Our results indicate that banks in MENA countries must strategically regulate bank political connections during crises and consistently thereafter. Our findings have implications for regulators investors and authorities in MENA countries.
Efficacy of high-frequency spinal cord stimulation for fibromyalgia syndrome in two cases: case reports
Authors: Horita Mikiko , Yasuhira Ayumi , Hirakawa Mikako , Watanabe Aisa , Higaki Nobuhiro , Nishihara Tasuku , Yorozuya Toshihiro 
Published: October 2023

Abstract
Background
Reports on the effectiveness of spinal cord stimulation (SCS) for the alleviation of fibromyalgia syndrome (FMS) pain are scarce. We report two cases of effective high-frequency SCS at 1000 Hz against upper- and lower-limb pain in patients with FMS.

Case presentation
Two women with widespread pain were diagnosed with FMS and the pain gradually worsened. A 1-week SCS trial was conducted in each patient. In both cases, the patients complained of unpleasant sensations during 10-Hz SCS. However, the pain was alleviated after 1000-Hz stimulation without irritation. Therefore, leads and a generator were implanted, after which they felt almost no pain. Moreover, the dose of the oral medication could be reduced and the patients returned to their daily lives.

Conclusion
SCS at 1000 Hz may effectively treat pain associated with FMS. Therefore, performing an SCS trial for patients with FMS with intractable pain might be worthwhile.
Standard experimental paradigm designs and data exclusion practices in cognitive psychology can inadvertently introduce systematic “shadow” biases in participant samples
Authors: Siritzky Emma M. , Cox Patrick H. , Nadler Sydni M. , Grady Justin N. , Kravitz Dwight J. , Mitroff Stephen R. 
Published: October 2023

AbstractStandard cognitive psychology research practices can introduce inadvertent sampling biases that reduce the reliability and generalizability of the findings. Researchers commonly acknowledge and understand that any given study sample is not perfectly generalizable, especially when implementing typical experimental constraints (e.g., limiting recruitment to specific age ranges or to individuals with normal color vision). However, less obvious systematic sampling constraints, referred to here as “shadow” biases, can be unintentionally introduced and can easily go unnoticed. For example, many standard cognitive psychology study designs involve lengthy and tedious experiments with simple, repetitive stimuli. Such testing environments may 1) be aversive to some would-be participants (e.g., those high in certain neurodivergent symptoms) who may self-select not to enroll in such studies, or 2) contribute to participant attrition, both of which reduce the sample’s representativeness. Likewise, standard performance-based data exclusion efforts (e.g., minimum accuracy or response time) or attention checks can systematically remove data from participants from subsets of the population (e.g., those low in conscientiousness). This commentary focuses on the theoretical and practical issues behind these non-obvious and often unacknowledged “shadow” biases, offers a simple illustration with real data as a proof of concept of how applying attention checks can systematically skew latent/hidden variables in the included population, and then discusses the broader implications with suggestions for how to manage and reduce, or at a minimum acknowledge, the problem.
The effect of nutrition and physical activity on sleep quality among adults: a scoping review
Authors: Alruwaili Nawaf W. , Alqahtani Nasser , Alanazi Maryam H. , Alanazi Bushra S. , Aljrbua Meznah S. , Gatar Othman M. 
Published: October 2023

AbstractSleep quality and its effects have become a public concern over the last few years. While the prevalence of sleep disorders was increasing, several studies have linked diet and physical activity as a cause of it. Indeed, many people complain about their sleeping problems without considering their lifestyle as a cause. This leads the efforts to focus on these principal factors and find their specific effect. The study aims to map out the research on the effect of nutrition and physical activity on sleep quality. The literature search was conducted in electronic libraries and databases related to nutrition and medical literature – Google Scholar, PubMed, and ScienceDirect– using relevant selected keywords. Article inclusion and selection were made by excluding duplicates, analyzing titles and abstracts, and reviewing the articles’ full text. This review included 61 articles. This literature study reinforces the importance of researching sleep and the lifestyle contributors to poor sleep, such as physical activity and nutrition.