bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot
Authors: Alegria Andrew D , Joshi Amey S , Mendana Jorge Blanco , Khosla Kanav , Smith Kieran T , Auch Benjamin , Donovan Margaret , Bischof John , Gohl Daryl M , Kodandaramaiah Suhasa B 
Published: February 2024

Abstract
Microinjection is a technique used for transgenesis, mutagenesis, cell labeling, cryopreservation, and in vitro fertilization in multiple single and multicellular organisms. Microinjection requires specialized skills and involves rate-limiting and labor-intensive preparatory steps. Here, we constructed a machine-vision guided generalized robot that fully automates the process of microinjection in fruit fly (Drosophila melanogaster) and zebrafish (Danio rerio) embryos. The robot uses machine learning models trained to detect embryos in images of agar plates and identify specific anatomical locations within each embryo in 3D space using dual view microscopes. The robot then serially performs a microinjection in each detected embryo. We constructed and used three such robots to automatically microinject tens of thousands of Drosophila and zebrafish embryos. We systematically optimized robotic microinjection for each species and performed routine transgenesis with proficiency comparable to highly skilled human practitioners while achieving up to 4× increases in microinjection throughput in Drosophila. The robot was utilized to microinject pools of over 20,000 uniquely barcoded plasmids into 1,713 embryos in 2 days to rapidly generate more than 400 unique transgenic Drosophila lines. This experiment enabled a novel measurement of the number of independent germline integration events per successfully injected embryo. Finally, we showed that robotic microinjection of cryoprotective agents in zebrafish embryos significantly improves vitrification rates and survival of cryopreserved embryos post-thaw as compared to manual microinjection. We anticipate that the robot can be used to carry out microinjection for genome-wide manipulation and cryopreservation at scale in a wide range of organisms.
Characteristics of and Variation in Suicide Mortality Related to Retirement During the Great Recession: Perspectives From the National Violent Death Reporting System
Authors: Ananthasubramaniam Aparna , Jurgens David , Kahsay Eskira , Mezuk Briana 
Published: February 2024

Abstract

Background and Objectives
Suicide rates typically increase during recessions. However, few studies have explored how recessions affect risk among older adults nearing retirement. This study used a large suicide mortality registry to characterize and quantify suicide related to retirement during the Great Recession (GR).

Research Design and Methods
Data come from the National Violent Death Reporting System (NVDRS, 2004–2017; N = 53,298 suicide deaths age ≥50). We analyzed the text narratives (i.e., descriptions of the most salient circumstances to each suicide) of these decedents using natural language processing (NLP) to identify cases that were “retirement-related” (RR, e.g., anticipating, being unable to, or recently retiring). We used time-series analysis to quantify variation in RR over the GR, and compared these trends to retirees (i.e., decedents whose occupation was “retired”) and all decedents aged ≥50. We used content and network analysis to characterize themes represented in the narratives.

Results
There were 878 RR cases (1.6% of suicides aged ≥50) identified by the NLP model; only 52% of these cases were among retirees. RR cases were younger (62 vs 75 years) and more educated (41.5% vs 24.5% college degree) than retirees. The rate of RR suicide was positively associated with indicators of the GR (e.g., short-term unemployment R2 = 0.70, p = .024), but economic indicators were not correlated with the suicide rate among retirees or older adults in general. Economic issues were more central to the narratives of RR cases during the GR compared to other periods.

Discussion and Implications
Recessions shape suicide risk related to retirement transitions.
Passive sustainability strategies in traditional Gaziantep residences: a critical report on historical development
Authors: Anaç Merve , Cuce Pinar Mert , Cuce Erdem 
Published: February 2024

Abstract
In recent years, the increase in the need for energy and the rapid depletion of energy resources have revealed that energy control in buildings is of vital importance. This situation requires energy efficiency not only in new buildings but also in existing building stocks. For this reason, passive strategic measures affecting thermal comfort and energy efficiency in historical buildings built in Gaziantep in the late 18th and early 19th centuries have been evaluated in this study. First, the cultural, technological and natural images of the region that are effective in the development of these strategies were identified. The reflection of these images on architectural design and the parameters affecting energy use as a result were revealed. In order to examine the effect of passive strategy measures in the spaces, a field study was carried out on a selected Gaziantep traditional residential building. For the field study, measurements were taken from the selected building and the street every hour during the day. These measurements have been made under the following conditions: sunny inside the courtyard, sunny outside the courtyard, shade inside the courtyard and shade outside the courtyard. Afterwards, all measurements have been compared. As a result, it is concluded that the courtyard systems that form the cultural, technological and natural image of the region have a great effect on thermal comfort.
Plasma MERTK Is a Promising Biomarker for the Diagnosis and Prognosis of Hepatitis B Virus–Related Acute-on-Chronic Liver Failure
Authors: Lu Yingyan , Xin Jiaojiao , Liang Xi , Luo Jinjin , Li Peng , Zhou Xingping , Yang Hui , Li Jun , Wang Yifan 
Published: February 2024

Abstract

Background
Hepatitis B virus–related acute-on-chronic liver failure (HBV-ACLF) has a high short-term mortality. This study aimed to determine the diagnostic and prognostic role of MER tyrosine kinase (MERTK) in patients with HBV-ACLF.

Methods
Transcriptomics analysis evaluated MERTK expression and function during disease progression. The diagnostic and prognostic significance of MERTK for patients with HBV-ACLF were verified by enzyme-linked immunosorbent assay, area under the receiver operating characteristic curve (AUROC) analysis, and immunohistochemistry (IHC) of liver tissues.

Results
MERTK mRNA was highly expressed in patients with HBV-ACLF compared to those with liver cirrhosis (LC), chronic hepatitis B (CHB), and normal controls (NC). Elevated MERTK mRNA predicted poor prognosis for HBV-ACLF at 28 and 90 days (AUROC = 0.814 and 0.731, respectively). Functional analysis showed MERTK was significantly associated with toll-like receptor and inflammatory signaling and several key biological processes. External validation with 285 plasma subjects confirmed the high diagnostic accuracy of plasma MERTK for HBV-ACLF (AUROC = 0.859) and potential prognostic value for 28- and 90-day mortality rates (AUROC = 0.673 and 0.644, respectively). Risk stratification analysis indicated higher mortality risk for patients with plasma MERTK level above the cutoff value. Moreover, IHC staining showed increasing MERTK expression from NC, CHB, and LC to HBV-ACLF.

Conclusions
MERTK shows promise as a candidate biomarker for early diagnosis and prognosis of HBV-ACLF.
Ontological Crisis and the Compartmentalization of Insecurities
Authors: Tsintsadze-Maass Eteri 
Published: February 2024

Abstract
This article explores state responses to a major ontological crisis, which produces insecurities requiring contradictory foreign policy responses. I propose that leaders in such dire situations may respond by compartmentalizing insecurities, articulating distinct narratives relevant to different insecurities. Such a split might seem inconsistent for leaders within the same government, but it can enable them to navigate a precarious crisis by exploiting the state’s internal complexity to address the contrasting insecurities that a crisis generates. I explore this approach by analyzing Georgia’s response to Russia’s full-scale invasion of Ukraine in February 2022, which created a major ontological crisis in Georgia. Its two most prominent leaders (the president and the prime minister) reacted by consistently voicing contradictory positions about the country’s foreign policy. This divergent messaging becomes comprehensible when we see them as responses to different concerns: one responding to deep ontological insecurities over Georgia’s relationship with other states, its place in the international system, and the coherence of its dominant autobiographical narrative; the other responding to the threat of war and related existential concerns at the individual and collective levels. Problematizing the state as a unitary actor, this article demonstrates how unpacking its constitutive agents can help us better understand how leaders navigate complex ontological crises.
Entomopathogenic pseudomonads can share an insect host with entomopathogenic nematodes and their mutualistic bacteria
Authors: Zwyssig Maria , Spescha Anna , Patt Tabea , Belosevic Adrian , Machado Ricardo A R , Regaiolo Alice , Keel Christoph , Maurhofer Monika 
Published: February 2024

Abstract
A promising strategy to overcome limitations in biological control of insect pests is the combined application of entomopathogenic pseudomonads (EPPs) and nematodes (EPNs) associated with mutualistic bacteria (NABs). Yet, little is known about interspecies interactions such as competition, coexistence, or even cooperation between these entomopathogens when they infect the same insect host. We investigated the dynamics of bacteria–bacteria interactions between the EPP Pseudomonas protegens CHA0 and the NAB Xenorhabdus bovienii SM5 isolated from the EPN Steinernema feltiae RS5. Bacterial populations were assessed over time in experimental systems of increasing complexity. In vitro, SM5 was outcompeted when CHA0 reached a certain cell density, resulting in the collapse of the SM5 population. In contrast, both bacteria were able to coexist upon haemolymph-injection into Galleria mellonella larvae, as found for three further EPP-NAB combinations. Finally, both bacteria were administered by natural infection routes i.e. orally for CHA0 and nematode-vectored for SM5 resulting in the addition of RS5 to the system. This did not alter bacterial coexistence nor did the presence of the EPP affect nematode reproductive success or progeny virulence. CHA0 benefited from RS5, probably by exploiting access routes formed by the nematodes penetrating the larval gut epithelium. Our results indicate that EPPs are able to share an insect host with EPNs and their mutualistic bacteria without major negative effects on the reproduction of any of the three entomopathogens or the fitness of the nematodes. This suggests that their combination is a promising strategy for biological insect pest control.
Keeping Up and Staying Fresh: Reflections on Studying Emerging Topics in Consumer Research
Authors: Stephen Andrew T 
Published: February 2024

AbstractThis article reflects on the study of consumer-related emerging topics in the Journal of Consumer Research (JCR) and looks ahead to consider what the next generation of emergent issues might be that capture consumer researchers’ attention and interest. Drawing on conversations with eminent scholars who have made major contributions to JCR by introducing emerging topics to our literature, the author (i) provides a commentary of past approaches to researching novel emerging topics, (ii) suggests some domains from which new emerging topics for consumer research can be sourced, such as new digital technologies (e.g., artificial intelligence) and major societal challenges (e.g., climate change, sustainability, and health), and (iii) offers guidance on how to approach research on important emerging topics.
A population-based validation of the IGCCCG Update Consortium for survival in metastatic non-seminoma testis cancer
Authors: Incesu Reha-Baris , Morra Simone , Scheipner Lukas , Barletta Francesco , Baudo Andrea , Garcia Cristina Cano , Tappero Stefano , Piccinelli Mattia Luca , Tian Zhe , Saad Fred , Shariat Shahrokh F , de Cobelli Ottavio , Terrone Carlo , Chun Felix K H , Carmignani Luca , Briganti Alberto , Ahyai Sascha , Longo Nicola , Tilki Derya , Graefen Markus , Karakiewicz Pierre I 
Published: February 2024

Abstract

Background
In 2021, the International Germ Cell Cancer Collaborative Group (IGCCCG) Update Consortium reported improved overall survival (OS) rates in a modern cohort of metastatic non-seminoma testis cancer patients within each of the IGCCCG prognosis groups (96% in good vs. 89% in intermediate vs. 67% in poor), compared to the previous IGCCCG publication (92% in good vs. 80% in intermediate vs. 48% in poor). We hypothesized that a similar survival improvement may apply to a contemporary North-American population-based cohort of non-seminoma testis cancer patients.

Patients and Methods
The Surveillance, Epidemiology, and End Results (SEER) database (2010–2018) was used. Kaplan–Meier plots and multivariable Cox regression models tested the effect of IGCCCG prognosis groups on overall mortality (OM).

Results
Of 1672 surgically treated metastatic non-seminoma patients, 778 (47%) exhibited good vs. 251 (15%) intermediate vs. 643 (38%) poor prognosis. In the overall cohort, five-year OS rate was 94% for good prognosis vs. 87% for intermediate prognosis vs. 65% for poor prognosis. In multivariable Cox regression models predicting OM, intermediate (Hazard ratio [HR] 2.4, 95% confidence interval [CI] 1.4–3.9, P &lt; 0.001) and poor prognosis group (HR 6.6, 95% CI 1.0–1.0, P &lt; 0.001) were independent predictors of higher OM, relative to good prognosis group.

Conclusions
The survival improvement reported by the IGCCCG Update Consortium is also operational in non-seminoma testis cancer patients within the most contemporary SEER database. This observation indicates that the survival improvement is not only applicable to centres of excellence, but also applies to other institutions at large.
Anlotinib and anti-PD-1 mAbs perfected CIK cell therapy for lung adenocarcinoma in preclinical trials
Authors: Lv Yingge , Zhao Hua , Liu Shaochuan , Meng Yuan , Yu Wenwen , Liu Ting , Sun Qian , Shen Meng , Ren Xiubao , Liu Liang 
Published: February 2024

Abstract
Murine cytokine-induced killer (CIK) cells are heterologous cells that kill various allogeneic and isogenic tumors and have functional and phenotypic characteristics of natural killer cells and T lymphocytes. However, the effect of CIK cells alone on solid tumor therapy is only limited. To enhance the therapeutic effect, it is vital to discover a mix of several therapy approaches. Immune cell function is inhibited by abnormal tumor vessels and the tumor microenvironment, which block lymphocyte entry into tumor tissue. To increase the effectiveness of CIK cells’ antitumor activity, antivascular therapy and CIK cell therapy can be combined. Furthermore, anlotinib is a tiny drug with multitarget tyrosine kinase inhibitors that can block cell migration, delay angiogenesis, and decrease blood vessel density. Compared with other antiangiogenesis drugs, anlotinib stands out due to the wider target of action and lower effective dose. In this work, anlotinib and murine CIK cells were coupled to boost CD3+ T cell infiltration, CD3+CD4+ T cell infiltration, and expression of granzyme B and interferon γ from CD3+CD8+ T cells, which increased the antitumor activity. Through the generation of cytotoxic cytokines by T lymphocytes, the therapeutic group using anti-PD-1 monoclonal antibodies in conjunction with anlotinib and CIK cells was more successful than the group receiving dual therapy. The preclinical study contributes to exploring the therapeutic alternatives for patients with lung adenocarcinoma, thus prolonging their lives.
Highlights of Veterinary Entomology, 2022
Authors: Hubbard Caleb B 
Published: February 2024

Abstract
The field of veterinary entomology is dominated by research concerning insects and arthropods that negatively impact the health of domestic animals. A curated selection of peer-reviewed research which was highlighted at the 2022 Joint Meeting between the Entomological Society of America, the Entomological Society of Canada, and the Entomological Society of British Columbia, which prioritized exploring entomology through the lens of art, science, and culture are summarized. Articles are categorized into (i) biting and non-biting flies, (ii) ectoparasites, and (iii) review articles, with the overall goal of showcasing innovative methodologies, addressing overlooked questions in veterinary entomology, and highlighting comprehensive reviews. While not exhaustive, the selected studies represent a myriad of arthropods, methodologies, and perspectives, to inspire future scientists with diverse research avenues and emphasize the continual evolution and importance of entomological studies in today’s world.
Ecological interactions of Triatoma sanguisuga (Hemiptera: Reduviidae) and risk for human infection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) in Illinois and Louisiana
Authors: Dumonteil Eric , Tu Weihong , Jiménez F Agustín , Herrera Claudia 
Published: February 2024

Abstract
Triatoma sanguisuga (Leconte) is one of the most widely distributed kissing bugs in the United States, associated with an extensive zoonotic circulation of Trypanosoma cruzi, the agent of Chagas disease, in a large part of the country. However, the actual risk for human infection in the United States is poorly understood. Here, we further assessed the ecology of T. sanguisuga bugs collected in residents’ houses in Illinois and Louisiana, using a metagenomic approach to identify their blood-feeding sources, T. cruzi parasites and gut microbiota. Blood meal analysis revealed feeding on domestic animals (dogs, cats, pigs, goats, and turkeys), synanthropic species (raccoons, opossums, and squirrels), as well as the more sylvatic white-tail deer. Human blood was identified in 11/14 (78%) of bugs, highlighting a frequent vector–human contact. The infection rate with T. cruzi was 53% (8/15), and most infected bugs (6/8) had fed on humans. A total of 41 bacterial families were identified, with significant differences in microbiota alpha and beta diversity between bugs from Louisiana and Illinois. However, predicted metabolic functions remained highly conserved, suggesting important constraints to fulfill their role in bug biology. These results confirmed a significant risk for vector-borne transmission of T. cruzi to humans in Louisiana and Illinois, which warrants more active screening for human infections. Also, while there is broad plasticity in the bacterial composition of T. sanguisuga microbiota, there are strong constraints to preserve metabolic profile and function, making it a good target for novel vector control strategies.
The stellar ‘Snake’ – II. The mass function
Authors: Yang Xiang-Ming , Bird Sarah A , Li Jiadong , Tian Hai-Jun , Qiu Dan , Li Jia-Peng , Li Cheng-Yuan , Liu Gao-Chao , Zhang Peng , Zhang Ju-Yong , Chen Zhi-Ping 
Published: February 2024

ABSTRACT
We present a comprehensive investigation on the mass function (MF) of a snake-like stellar structure in the solar neighbourhood, building on our previous discovery. To ensure the reliability of the data, we reselect the member stars of the Stellar ‘Snake’ in the latest Gaia Data Release 3 using the same approach as the initial series of articles. We also precisely measure the physical parameters of the clusters within the Stellar Snake. In light of the high completeness of the member stars in the cluster regions, we develop a simulated model colour–magnitude diagram-based inference method to derive the mass function, binary fraction, and mass-ratio distribution of the clusters in the Stellar Snake. Notably, despite their similar ages and metallicity, we discover systematic variations in the MFs along the elongation direction of the Snake in the mass range of 0.5 to 2.0 M⊙. The ‘head’ of the Snake conforms to a canonical initial mass function with a power-law slope of α ∼ −2.3. Extending towards the ‘tail’, the MF becomes more top-light, indicating a deficiency of massive stars within these clusters. This result provides evidence for the delayed formation of massive stars in the clusters. Such clues give support to the hypothesis that the Stellar Snake constitutes as a hierarchically primordial structure.
Survey to assess the feasibility of establishing an international network for evidence synthesis in occupational safety and health
Authors: McElvenny D M , Verbeek J , Gagliardi D , Tikka C , Hoving J L 
Published: February 2024

Abstract

Background
Evidence synthesis in the field of occupational safety and health (OSH) has been continuously growing over the last two decades. With over 100 systematic reviews now published, the Cochrane Work Review group has played an important role in this development and the Cochrane Thematic Group ‘Work & Health & Social Security’ was established recently to combine evidence from both the OSH and insurance medicine fields. Worldwide, many organizations produce and synthesize evidence in OSH that can complement and support each other. We believe that a global network including Cochrane and others can collaborate on methods development and in the production, synthesis, use and dissemination of different types of evidence even more effectively.

Aims
To determine if establishing a global network for evidence synthesis in OSH is feasible.

Methods
We conducted a survey of international and national institutions between November 2022 and January 2023 using LimeSurvey. Participants included representatives of affiliated and sustaining members of the International Commission on Occupational Health, national institutes for OSH, academia and other international organizations.

Results
From 151 invitations, we received responses from 57 representatives of 54 organizations. Representatives reported that their organization will contribute financially on an annual basis (n = 1) or provide in-kind support (n = 10), and will probably be able to provide financial or in-kind support (n = 25).

Conclusions
The feasibility criterion was met and an international network is being established.