bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐term freedom from recurrence in 2 stage IV melanoma patients following vaccination with tyrosinase peptides
Authors: Scheibenbogen Carmen , Nagorsen Dirk , Seliger Barbara , Schmittel Alexander , Letsch Anne , Bauer Sandra , Max Nicole , Bock Michaela , Atkins Derek , Thiel Eckhard , Keilholz Ulrich 
Published: August 2002

AbstractWe report here on 2 patients who received adjuvant vaccination with an HLA‐A2– or HLA‐A24–restricted tyrosinase peptide, respectively, and GM‐CSF for frequently relapsing stage IV melanoma. Following resection of metastases and irradiation of brain metastases in 1 patient, both patients were without evidence of disease when receiving the first vaccination. While the patients had had 9 and 12, respectively, mostly s.c., relapses during the 3 years before vaccination, they experienced freedom from relapse for more than 2 years after vaccination. We found a T‐cell response to the vaccine peptide in both patients in the peripheral blood by ex vivo IFN‐γ ELISPOT assay. The T‐cell population could be further characterized by 4‐color flow cytometry in 1 patient, showing that the majority of the peptide‐specific CD3+CD8+IFN‐γ+ T cells were granzyme B‐positive and CCR‐7‐negative, characterizing them as effector T cells with the ability to mediate cytotoxicity and migrate to inflamed tissues. In this patient also, augmentation of the T‐cell response to autologous tumor cells by vaccination could be detected. A single‐site postvaccination relapse occurred in both patients, showing downregulation of tyrosinase expression in 1 patient, while normal expression levels for tyrosinase, MHC class I antigens and components of the antigen‐processing machinery were found in the other patient. These results suggest that peptide vaccination resulted in a prolonged relapse‐free interval in these high‐risk patients. © 2002 Wiley‐Liss, Inc.
Leukaemia and non‐Hodgkin's lymphoma in children of male Sellafield radiation workers
Authors: Dickinson Heather O. , Parker Louise 
Published: August 2002

AbstractOur objective was to investigate if there was (i) an excess risk of leukaemia/non‐Hodgkin's lymphoma among children of male radiation workers at the Sellafield nuclear installation in Cumbria, northwest England; (ii) a dose‐response relationship between fathers' preconceptional irradiation and their children's risk of leukaemia/non‐Hodgkin's lymphoma; and (iii) whether any observed association could be explained by demographic factors. We performed a cohort study of live births, 1950–1991 in Cumbria, followed up to age 25 years or the end of 1991, comparing the risk of leukaemia/non‐Hodgkin's lymphoma among all 9,859 children of male radiation workers to that among all 256,851 children of non‐Sellafield fathers. Children of radiation workers had a higher risk of leukaemia/non‐Hodgkin's lymphoma than other children [rate ratio (RR) = 1.9, 95% confidence interval (CI) 1.0–3.1, p = 0.05]. Adjustment for population mixing greatly reduced the excess risk in the village of Seascale, adjacent to Sellafield, but had little effect elsewhere. The risk increased significantly with father's total preconceptional external radiation dose (RR100mSv = 1.6, 95% CI 1.0–2.2, p = 0.05). This dose‐response was not reduced by adjustment for population mixing. Although our 13 exposed cases included 10 considered previously (Gardner et al., BMJ 1990;300:423–34), we used a cohort rather than a case‐control design, with wider temporal and geographic boundaries, and confirmed the statistical association between father's preconceptional irradiation and child's risk of leukaemia/non‐Hodgkin's lymphoma that they reported. The possibility remains that paternal preconceptional irradiation may be a risk factor for leukaemia/non‐Hodgkin's lymphoma, and this effect may not be confined to Seascale. © 2002 Wiley‐Liss, Inc.
Prediction of response to docetaxel by CYP3A4 mRNA expression in breast cancer tissues
Authors: Miyoshi Yasuo , Ando Akiko , Takamura Yuuki , Taguchi Tetsuya , Tamaki Yasuhiro , Noguchi Shinzaburo 
Published: August 2002

AbstractWe studied the relationship between response of breast cancer to docetaxel (DOC) or cylophosphamide + epirubucin (CE) treatment and CYP3A4 mRNA expression in breast tumors. CYP3A4 inactivates DOC but not E, which is a predominant effector in CE treatment. Twenty patients with locally advanced breast tumors and 18 patients with locally recurrent tumors underwent tumor biopsy before chemotherapy, and CYP3A4 mRNA expression levels in tumor tissues were assayed by real‐time quantitative polymerase chain reaction. Twenty‐three patients were treated with DOC (60 mg/m2 q3w) and 15 patients were treated with CE (C 600 mg/m2 and E 60 mg/m2 q3w). Patients with low CYP3A4 mRNA levels (n = 14) exhibited a significantly (p < 0.01) higher response rate (71%) to DOC treatment than those (n = 9) with high CYP3A4 mRNA levels (response rate, 11%). Positive predictive value, negative predictive value and diagnostic accuracy of CYP3A4 mRNA levels in the prediction of response to DOC were 71, 89, and 78%, respectively. However, no significant association was observed between CYP3A4 mRNA expression and response to CE treatment. These results suggest that intratumoral CYP3A4 mRNA levels might be useful as a predictor of response to DOC treatment, but not to CE treatment, in breast cancer patients. The increased inactivation of DOC by CYP3A4 in tumor tissues may play some role in the acquisition of resistance to DOC. © 2002 Wiley‐Liss, Inc.
Incidence and mortality trends for prostate cancer in 5 French areas from 1982 to 1996
Authors: Chirpaz Emmanuel , Colonna Marc , Menegoz François , Grosclaude Pascale , Schaffer Paul , Arveux Patrick , Lesec'h Josette Mace , Exbrayat Catherine , Schaerer René 
Published: August 2002

AbstractAfter an increase in the 1980s, incidence and mortality for prostate cancer in North America or England and Wales started to decrease in the early 1990s. The reasons for this evolution are widely debated, notably the importance of early detection. This study describes trends of prostate cancer incidence and mortality in 5 areas in France, where practices of early detection for this cancer are widely used. The 5 French administrative areas, covered by a population‐based registry, have a total population of approximately 1,700,000 men. Incidence data from these registries were studied for the period 1982–1995, and mortality data were provided by the Institut National de la Santé et de la Recherche Médicale (INSERM) for the period 1982–1996. Age‐Period‐Cohort models by Poisson regression were created to characterize these trends. Between 1982 and 1995, 14,699 cases of prostate cancer were registered by the 5 registries under consideration. After a little intensification of the increase in 1987, undoubtedly due to early detection (notably using Prostate‐Specific Antigen), the trend of the incidence seems to reverse from 1993. Mortality increased monotonically from 1982–1990 by an average of 1.8% per year, before decreasing annually by an average of 3.3% until 1996. Poisson regressions indicated a period effect on both incidence and mortality data; a small, but significant, cohort effect exists for incidence evolution, showing that elements such as etiologic factors may have an influence. Until results of randomized studies on mass screening are available, the question of individual screening remains; improved knowledge of risk factors could be interesting. © 2001 Wiley‐Liss, Inc.
The role of training and technical assistance in supporting the delivery of high quality services in Early Head Start
Authors: Mann Tammy L. 
Published: August 2002

AbstractTraining and technical assistance services have been an integral part of the fabric of support that Early Head Start (EHS) programs have utilized as they have worked to implement quality programs. In the same way that EHS has continued to evolve with increased program expansion, so too have the training and technical assistance support services. This article examines that nature of the early system of support and notes challenges that EHS programs faced during their first year of funding and the impact of these challenges on training and technical assistance support. The present day system is composed of a network of national and regional providers and federal staff working in partnership to support EHS programs. The nature of this network is described and recommendations for future areas of emphasis for training and technical assistance support are offered. ©2002 Michigan Association for Infant Mental Health.
Water resources management in the Aral Basin: a river basin management model for the Syr Darya
Authors: Heaven S. , Koloskov G. B. , Lock A. C. , Tanton T. W. 
Published: August 2002

AbstractThe Aral Sea environmental crisis has been created by poor water management in the river basins of the Amu Darya and Syr Darya. Nearly all the flow is abstracted for ailing irrigation schemes in the upper and middle basins, for massive river basin transfer schemes, or is lost to evaporation and seepage from reservoirs and canals. The only solution to the crisis in the lower deltas is to allow more water to flow to them. The paper argues that the only practical approach for achieving this in the short to medium term is better water management in the upper and middle river basins, and that a more rational basis for allocating water to meet the needs of all users is urgently required. The paper describes a real‐time water resources management model for the Syr Darya river that has been designed for sustainable planning and management of the water resources to meet the needs of all riparian users. The power and limitations of such models in relation to effective management of the water resources of the Aral Basin are discussed. Copyright © 2002 John Wiley & Sons, Ltd.
Haemolytic anaemia after oral self‐giving of naphthalene‐containing oil
Authors: Trevisan Andrea , di Schio Margherita Rossi , Pieno Mariangela 
Published: August 2002

AbstractA clinical case of suicide attempt by means of oral self‐giving of naphthalene‐containing oil in an old man was reported. Clinical features showed haemolytic anaemia supported by a decrease of haemoglobin concentration and red blood cell count, an increase of bilirubin (prevalently indirect) and lactate dehydrogenase and very low levels of haptoglobin. Methaemoglobin (metHb) measured at admission to the Emergency Room and 19 days after poisoning was still above normal limits. No deficiency of glucose‐6‐phosphate dehydrogenase was detected. The clinical manifestations ameliorated after treatment with concentrated red blood cells and ascorbic acid, with fast normalization of metHb. The clinical picture appeared almost normal 1 month after poisoning. The clinical diagnosis was ‘haemolytic anaemia caused by naphthalene’. Absence of glucose‐6‐phosphate dehydrogenase deficiency probably reduced the severity of poisoning. Copyright © 2001 John Wiley & Sons, Ltd.
Surface modification by alkali and heat treatments in titanium alloys
Authors: Lee Baek‐Hee , Do Kim Young , Shin Ji Hoon , Hwan Lee Kyu 
Published: August 2002

AbstractPure titanium and titanium alloys are normally used for orthopedic and dental prostheses. Nevertheless, their chemical, biological, and mechanical properties still can be improved by the development of new preparation technologies. This has been the limiting factor for these metals to show low affinity to living bone. The purpose of this study is to improve the bone‐bonding ability between titanium alloys and living bone through a chemically activated process and a thermally activated one. Two kinds of titanium alloys, a newly designed Ti‐In‐Nb‐Ta alloy and a commercially available Ti‐6Al‐4V ELI alloy, were used in this study. In this study, surface modification of the titanium alloys by alkali and heat treatments (AHT), alkali treated in 5.0M NaOH solution, and heat treated in vacuum furnace at 600°C, is reported. After AHT, the effects of the AHT on the bone integration property were evaluated in vitro. Surface morphologies of AHT were observed by optical microscopy (OM) and scanning electron microscopy (SEM). Chemical compositional surface changes were investigated by X‐ray diffractometry (XRD), energy dispersive spectroscopy (EDS), and auger electron spectroscopy (AES). Titanium alloys with surface modification by AHT showed improved bioactive behavior, and the Ti‐In‐Nb‐Ta alloy had better bioactivity than the Ti‐6Al‐4V ELI alloy in vitro. © 2002 Wiley Periodicals, Inc. J Biomed Biomater Res 61: 466–473, 2002
Cloning, sequencing, heterologous expression, and characterization of murine cytochrome P450 3a25*(Cyp3a25), a testosterone 6β‐hydroxylase
Authors: Dai Ding , Bai Re , Hodgson Ernest , Rose Randy L. 
Published: August 2002

AbstractA full‐length cDNA clone encoding a novel form of the cytochrome P450 3A subfamily (Cyp3a‐25) has been isolated from a mouse liver cDNA library. The sequence contained 2010 base pairs and encoded a protein with 503 amino acids. The amino acid sequence shared greater identities with rat CYP3A18 (90%) and golden hamster CYP3A10 (81%) sequences than with known mouse sequences (Cyp3a‐11, Cyp3a‐13, Cyp3a‐16, and Cyp3a‐41 [68–70%]). CYP3A25 was expressed in the Escherichia coli PCWori+ expression vector following slight modifications of the N‐ and C‐terminals of the cDNA. The purified CYP3A25 was recognized on an immunoblot by CYP3A1 antibody and has a molecular weight of 50 kD. CYP3A25 was catalytically active in the 6β‐hydroxylation of testosterone and the N‐demethylation of benzphetamine and erythromycin. It was demonstrated by RT‐PCR that the CYP3A25 mRNA is present in both fetal and adult tissues, including liver, lung, intestines, kidney, and brain. Northern blotting demonstrated that expression is greatest in the liver and small intestine. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:90–99, 2001
Signaling by the Mpl receptor involves IKK and NF‐κB*
Authors: Zhang Ying , Sun Shishinn , Wang Zhengyu , Thompson Alexander , Kaluzhny Yulia , Zimmet Jeffrey , Ravid Katya 
Published: August 2002

AbstractBinding of tumor necrosis factor‐α (TNF‐α) to its receptor activates IKK complex, which leads to inducement of NF‐κB activity. Here we report that activation of Mpl ligand is also linked to IKK and NF‐κB activity. Mpl ligand, also known as thrombopoietin (TPO) or megakaryocyte growth and development factor (MGDF), induces megakaryocyte differentiation and inhibition of mitotic proliferation, followed by induction of polyploidization and fragmentation into platelets. The latter process is often observed in megakaryocytes undergoing apoptosis. Treatment of a Mpl ligand‐responding megakaryocytic cell line with this cytokine led to an immediate, transient increase in IKK activity followed by a profound decrease in this kinase activity over time. This decrease was not due to an effect on the levels of the IKK regulatory components IKKα and IKKβ. Proliferating megakaryocytes displayed a constitutive DNA‐binding activity of NF‐κB p50 homodimers and of NF‐κB p50–p65 heterodimers. As expected, reduced IKK activity in Mpl ligand‐treated cells was associated with a significant reduction in NF‐κB DNA binding activity and in the activity of a NF‐κB‐dependent promoter. Our study is thus the first to identify a constitutive NF‐κB activity in proliferating megakaryocytes as well as to describe a link between Mpl receptor signaling and IKK and NF‐κB activities. Since a variety of proliferation‐promoting genes and anti‐apoptotic mechanisms are activated by NF‐κB, retaining its low levels would be one potential mechanism by which inhibition of mitotic proliferation is maintained and apoptosis is promoted during late megakaryopoiesis. J. Cell. Biochem. 85: 523–535, 2002. © 2002 Wiley‐Liss, Inc.
Analysis of regulator of G‐protein signaling‐2 (RGS‐2) expression and function in osteoblastic cells
Authors: Thirunavukkarasu Kannan , Halladay David L. , Miles Rebecca R. , Geringer Chad D. , Onyia Jude E. 
Published: August 2002

AbstractRegulator of G‐protein signaling‐2 (RGS‐2) belongs to a novel family of GTPase‐activating proteins that rapidly turn‐off G‐protein coupled receptor signaling. RGS proteins contain a characteristic RGS domain by which they interact with the α‐subunit of G‐proteins and drive them into their inactive GDP‐bound forms. Previously, we have reported that RGS‐2 mRNA is rapidly and transiently increased by PTH in rat bone and in osteoblast cultures in vitro. In this study, we further explored the molecular basis for the regulation of RGS‐2 by cloning and functionally characterizing the RGS‐2 gene promoter. We cloned 2.3‐ and 2.8‐kb fragments of the 5′‐flanking regions of the rat and mouse RGS‐2 genes, respectively, and generated a stable clone of UMR106 osteoblastic cells containing the rat RGS‐2 promoter driving the β‐gal reporter gene (p2.3RGS‐2‐β‐gal). Treatment of the stable clone with PTH resulted in a maximal 2.2‐ to 3.6‐fold increase in promoter activity at 8 h, reminiscent of the early response observed with endogenous RGS‐2 mRNA regulation. Further, PTH (1–38), (1–31), PTHrP (1–34), and forskolin, which elevate cAMP levels, stimulated the promoter, while PTH (3–34) and (7–34), which do not readily stimulate cAMP accumulation, and PMA that directly activates protein kinase C, had no effect on promoter activity. Taken together, these results implicate the involvement of the Gαs‐adenylate cyclase‐protein kinase A pathway in stimulating RGS‐2 expression. Maintenance of a hyperphosphorylated state via the inhibition of type 2A protein phosphatases by okadaic acid, resulted in a strong dose‐dependent increase in transcriptional activity of the RGS‐2 promoter as well as that of the endogenous RGS‐2 gene. Furthermore, overexpression of the osteoblast‐specific transcription factor Runx2 also led to a stimulation of RGS‐2 promoter activity. Functional analysis using RGS‐2 overexpression suggests the potential negative regulatory effects of RGS‐2 on PTH‐ and forskolin‐induced cAMP production in osteoblastic cells. In summary, our data suggest that PTH treatment results in a direct transcriptional stimulation of RGS‐2 that in turn may play a role in modulating the duration/intensity of PTH receptor signaling. J. Cell. Biochem. 85: 837–850, 2002. © 2002 Wiley‐Liss, Inc.
Dependence of relativistic effects on electronic configuration in the neutral atoms of d‐ and f‐block elements
Authors: Autschbach J. , Siekierski S. , Seth M. , Schwerdtfeger P. , Schwarz W. H. E. 
Published: August 2002

AbstractAlthough most neutral d‐ and f‐block atoms have ndg−2(n + 1)s2 and (n − 1)fg−2(n + 1)s2 ground configurations, respectively, where g is the group number (i.e., number of valence electrons), one‐third of these 63 atoms prefer a higher d‐population, namely via (n + 1)s→nd “outer” to “inner” electron shift (particularly atoms from the second d‐row), or via (n − 1)f→nd “inner” to “outer” electron shift (particularly atoms from the second f‐row). Although the response to the modified self‐consistent field is orbital destabilization and expansion for (n + 1)s→nd, and stabilization and contraction for (n − 1)f→nd, the relativistic modification of the valence orbital responses is stabilization in both cases. This is explained by double perturbation theory. Accordingly, electron configuration and relativity trigger the orbital energies, the orbital populations and the chemical shell effects in different ways. The particularly pronounced relativistic effects in groups 10 and 11, the so‐called gold maximum, occur because of particularly efficient cooperative nonrelativistic shell effects and relativistic stabilization effects (inverse indirect effect) at the end of the d‐block. © 2002 Wiley Periodicals, Inc. J Comput Chem 23: 804–813, 2002
Ureaplasma urealyticum biovar determination in women attending a family planning clinic in Guiné‐Bissau, using polymerase chain reaction of the multiple‐banded antigen gene
Authors: Domingues D. , Tavira L. Távora , Duarte A. , Sanca A. , Prieto E. , Exposto F. 
Published: August 2002

AbstractAlthough Ureaplasma urealyticum is commonly found in the genital tract of asymptomatic women, it has been suggested that only certain subgroups of this microorganism are disease associated. Vaginal specimens were collected to determine the distribution of U. urealyticum biovars and to estimate their possible association with age, absence of lactobacilli, and tetracycline resistance. Of the 94 women studied, 40 (43%) carried U. urealyticum and were biotyped by polymerase chain reaction (PCR). Twenty‐nine (73%) strains presented with parvo biovar, 10 (25%) with T960 biovar, and one (2.5%) with both biovars. Parvo biovar was predominant in all age groups and appears to be more frequent in women 20–25 years of age (41%), whereas T960 was common in women 30–35 years of age (22%). In this study, U. urealyticum was not associated with changes in vaginal flora, although the inverse apparently was true for Mycoplasma hominis. However, T960 biovar was more associated with the loss of lactobacilli than was parvo biovar. The number of T960 biovar strains that presented tetracycline (40%) or multiple (100%) resistance was higher than that of parvo biovar strains (27% and 69%, respectively). J. Clin. Lab. Anal. 16:71–75, 2002. © 2002 Wiley‐Liss, Inc.
Personality characteristics associated with psychological reactance
Authors: Seibel Cynthia A. , Dowd E. Thomas 
Published: August 2002

AbstractThis study compared six different personality disorders on level of psychological reactance. Eighty clients met criteria for inclusion in one of six personality disorder groups, including passive‐aggressive, dependent, personality disorder NOS (not otherwise specified), no personality disorder, obsessive‐compulsive, and borderline. Instruments included the Therapeutic Reactance Scale (TRS) and the Questionnaire for the Measurement of Psychological Reactance (QMPR). Four one‐way analyses of variance (ANOVAs) were conducted on the scales and subscales of the two reactance measures. All four ANOVAs were significant, although post hoc tests indicated significant differences only among the more extreme groups. The personality disorders were generally ordered on reactance level according to predictions derived from the theory of separation‐individuation. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 963–969, 2001.
Progressive muscle relaxation, breathing exercises, and ABC relaxation theory
Authors: Matsumoto Mia , Smith Jonathan C. 
Published: August 2002

AbstractThis study compared the psychological effects of Progressive Muscle Relaxation (PMR) and breathing exercises. Forty‐two students were divided randomly into two groups and taught PMR or breathing exercises. Both groups practiced for five weeks and were given the Smith Relaxation States Inventory before and after each session. As hypothesized, PMR practitioners displayed greater increments in relaxation states (R‐States) Physical Relaxation and Disengagement, while breathing practitioners displayed higher levels of R‐State Strength and Awareness. Slight differences emerged at Weeks 1 and 2; major differences emerged at Weeks 4 and 5. A delayed and potentially reinforcing aftereffect emerged for PMR only after five weeks of training—increased levels of Mental Quiet and Joy. Clinical and theoretical implications are discussed. © 2001 John Wiley & Sons, Inc. J Clin Psychol 57: 1551–1557, 2001.