bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired cortical neuronal homeostasis and cognition after diffuse traumatic brain injury are dependent on microglia and type I interferon responses
Authors: Packer Jonathan M. , Bray Chelsea E. , Beckman Nicolas B. , Wangler Lynde M. , Davis Amara C. , Goodman Ethan J. , Klingele Nathaniel E. , Godbout Jonathan P. 
Published: November 2023

AbstractNeuropsychiatric complications including depression and cognitive decline develop in the years after traumatic brain injury (TBI), negatively affecting quality of life. Microglial and type 1 interferon (IFN‐I) responses are associated with the transition from acute to chronic neuroinflammation after diffuse TBI in mice. Thus, the purpose of this study was to determine if impaired neuronal homeostasis and increased IFN‐I responses intersected after TBI to cause cognitive impairment. Here, the RNA profile of neurons and microglia after TBI (single nucleus RNA‐sequencing) with or without microglia depletion (CSF1R antagonist) was assessed 7 dpi. There was a TBI‐dependent suppression of cortical neuronal homeostasis with reductions in CREB signaling, synaptogenesis, and synaptic migration and increases in RhoGDI and PTEN signaling (Ingenuity Pathway Analysis). Microglial depletion reversed 50% of TBI‐induced gene changes in cortical neurons depending on subtype. Moreover, the microglial RNA signature 7 dpi was associated with increased stimulator of interferon genes (STING) activation and IFN‐I responses. Therefore, we sought to reduce IFN‐I signaling after TBI using STING knockout mice and a STING antagonist, chloroquine (CQ). TBI‐associated cognitive deficits in novel object location and recognition (NOL/NOR) tasks at 7 and 30 dpi were STING dependent. In addition, TBI‐induced STING expression, microglial morphological restructuring, inflammatory (Tnf, Cd68, Ccl2) and IFN‐related (Irf3, Irf7, Ifi27) gene expression in the cortex were attenuated in STINGKO mice. CQ also reversed TBI‐induced cognitive deficits and reduced TBI‐induced inflammatory (Tnf, Cd68, Ccl2) and IFN (Irf7, Sting) cortical gene expression. Collectively, reducing IFN‐I signaling after TBI with STING‐dependent interventions attenuated the prolonged microglial activation and cognitive impairment.
Subjective memory complaints: Prevalence, associated factors and sex differences in the ELSI‐Brazil study
Authors: Pinho Pedro J. M. R. , Bertola Laiss , Ramos Ari Alex , Ghossain Barbosa Matheus , Rabelo Wendell , Castro‐Costa Érico , Lima‐Costa Maria Fernanda , Ferri Cleusa Pinheiro 
Published: November 2023

AbstractBackgroundSubjective memory complaints (SMCs) are a possible prodrome of cognitive decline but are understudied in low‐ and middle‐income countries (LMIC). We aimed to estimate the prevalence of SMCs in a large, nationally representative sample of older adults from Brazil and to identify sociodemographic and health‐related factors that are associated with SMCs independently of objective memory.MethodsBaseline data (n = 7831) from the ELSI‐Brazil study, a national representative sample of adults aged 50 and over. They were asked to rate their memory and then divided into two groups ‐ having or not having SMCs. Logistic regression models were used to estimate the association of demographic characteristics, health related factors, objective memory performance and disability in basic (b‐ADL), instrumental (i‐ADL), and advanced (a‐ADL) activities of daily living associated with SMCs. Whether sex was an effect modifier of the association between age and objective memory performance and SMCs was also tested.ResultsOf the sample, 42% (95% CI; 39.9–43.9) had SMCs, and it was higher among women (46.9%) than men (35.9%). SMC prevalence decreased with age among women and increased among men, and for both it decreased with better cognitive performance. Fully adjusted logistic regression model showed that older age, higher education, higher b‐ADL scores, and better cognitive performance were associated with decreased SMCs, while being female, with higher number of chronic conditions, higher i‐ADL scores, worst self‐rated health, and an increased number of depressive symptoms were associated with increased SMCs. However, the interaction test (p < 0.001) confirmed that increased age was associated with decreased SMCs only among women, and that better objective memory performance was associated with decreased SMCs only among men.ConclusionsSMCs are common in the Brazilian older population and are associated with health and sociodemographic factors, with different patterns between men and women. There is a need for future studies looking at the cognitive trajectory and dementia risk in older adults with subjective cognitive complaints.
Numerical investigation of hydrothermal performance over perforated conical pin heat sinks
Authors: Al‐Karooshi Mohammed A. , Chahrour Khaled M. , Khalil Wissam H. , Al‐Damook Amer 
Published: November 2023

AbstractOver the past few decades, researchers have shown significant interest in enhancing the thermal efficiency of heat sinks while simultaneously increasing the power generation capacity of electronic devices and reducing their size. In this study, the focus lies on the originality of employing conical perforated pin heat sinks with multiple perforations (N = 0, 1, 2, and 3) and various conical pins inclination angles (Φ = 0°, 1°, 2°, 3°, and 4°). The study aimed to numerically investigate the effects of a perforated conical pin and cone inclination angle on heat transfer, pressure drop, CPU temperature, and hydrothermal performance (HTP) across the heat sinks using a three‐dimensional, turbulent flow as k–ω SST model combined with the thermal conjugate model. A validated CFD model is employed to conduct a parametric analysis of the effects of the quantity and placement of circular holes. A summary of the results reveals that Model B3 exhibited the highest HTP value, reaching approximately 1.15 at U = 10 m/s, with a commendable reduction in heat sink mass of over 18%. Ultimately, the perforated conical pin heat sink demonstrates the potential to fulfill the primary objective of this investigation, which is achieving an overall improvement in Nusselt number, CPU temperature, pressure drop, and reduced heat sink mass.
Bladder cancer with urinary diversion by a sigmoid colon conduit after transverse colon stoma
Authors: Aoki Tadashi , Furuya Ryota , Fukasawa Masanari , Nozawa Munehiro , Takihana Yoshio , Sudoh Makoto , Nakagomi Hiroshi 
Published: November 2023

IntroductionSigmoid conduit is one of the methods for achieving urinary diversion, but it is performed less frequently than ileal conduit and ureterostomy. Herein, we report a case in which a sigmoid colon conduit was performed after nephrostomy and transverse colostomy.Case presentationA 70‐year‐old man was referred to our hospital because of a bladder tumor. Computed tomography and transurethral biopsy revealed advanced bladder cancer with ureteral and rectal invasion. Despite drug therapy, the tumor progressed. Thus, nephrostomy and transverse colostomy were performed for urinary and fecal diversion, respectively. Subsequently, chemotherapy was administered for 8 months. As nephrostomy‐related complications occurred frequently during chemotherapy, a sigmoid colon conduit was performed instead of nephrostomy for urinary diversion to improve the patient's quality of life.ConclusionIn patients with advanced bladder cancer requiring a double stoma of the urinary and fecal tracts, sigmoid colon conduit may be selected as a urinary diversion method.
CAPM and a new investment decision method
Authors: Taussig Roi D. 
Published: November 2023

AbstractThe traditional CAPM explains the return on each security, while the current study suggests a new methodology for “picking” a broad index, based on regression trees. Analysts’ recommendations on market prices are analyzed, and a one to three‐stage method is employed. The analysis suggests a few rules of thumb (only one to three stages) for buying or selling the CRSP US market index. The findings are robust, and the rules are reliable. Researchers and practitioners may benefit greatly from the new rules.
Exercise attenuates polyglutamine‐mediated neuromuscular degeneration in a mouse model of spinal and bulbar muscular atrophy
Authors: Hirunagi Tomoki , Nakatsuji Hideaki , Sahashi Kentaro , Yamamoto Mikiyasu , Iida Madoka , Tohnai Genki , Kondo Naohide , Yamada Shinichiro , Murakami Ayuka , Noda Seiya , Adachi Hiroaki , Sobue Gen , Katsuno Masahisa 
Published: November 2023

AbstractBackgroundSpinal and bulbar muscular atrophy (SBMA) is a hereditary neuromuscular disorder caused by the expansion of trinucleotide cytosine–adenine–guanine (CAG) repeats, which encodes a polyglutamine (polyQ) tract in the androgen receptor (AR) gene. Recent evidence suggests that, in addition to motor neuron degeneration, defective skeletal muscles are also the primary contributors to the pathogenesis in SBMA. While benefits of physical exercise have been suggested in SBMA, underlying mechanism remains elusive.MethodsWe investigated the effect of running exercise in a transgenic mouse model of SBMA carrying human AR with 97 expanded CAGs (AR97Q). We assigned AR97Q mice to exercise and sedentary control groups, and mice in the exercise group received 1‐h forced running wheel (5 m/min) 5 days a week for 4 weeks during the early stage of the disease. Motor function (grip strength and rotarod performance) and survival of each group were analysed, and histopathological and biological features in skeletal muscles and motor neurons were evaluated.ResultsAR97Q mice in the exercise group showed improvement in motor function (~40% and ~50% increase in grip strength and rotarod performance, respectively, P < 0.05) and survival (median survival 23.6 vs. 16.7 weeks, P < 0.05) with amelioration of neuronal and muscular histopathology (~1.4‐fold and ~2.8‐fold increase in motor neuron and muscle fibre size, respectively, P < 0.001) compared to those in the sedentary group. Nuclear accumulation of polyQ‐expanded AR in skeletal muscles and motor neurons was suppressed in the mice with exercise compared to the sedentary mice (~50% and ~30% reduction in 1C2‐positive cells in skeletal muscles and motor neurons, respectively, P < 0.05). We found that the exercise activated 5′‐adenosine monophosphate‐activated protein kinase (AMPK) signalling and inhibited mammalian target of rapamycin pathway that regulates protein synthesis in skeletal muscles of SBMA mice. Pharmacological activation of AMPK inhibited protein synthesis and reduced polyQ‐expanded AR proteins in C2C12 muscle cells.ConclusionsOur findings suggest the therapeutic potential of exercise‐induced effect via AMPK activation in SBMA.
Regioselective synthesis of new imidazo[4,5‐e][1,3]thiazino[2,3‐c][1,2,4]triazines via reaction of imidazo[4,5‐e][1,2,4]triazinethiones with ethyl phenylpropiolate
Authors: Vinogradov Dmitry B. , Izmest'ev Alexei N. , Kravchenko Angelina N. , Kolotyrkina Natalya G. , Gazieva Galina A. 
Published: November 2023

AbstractA method for the regioselective synthesis of imidazo[4,5‐e][1,3]thiazino[2,3‐c][1,2,4]triazine derivatives has been developed by the reaction of imidazotriazinethiones with ethyl phenylpropiolate upon treatment with potassium carbonate or sodium methoxide in methanol. The synthesis was accomplished by Michael‐type addition of the imidazotriazinethiones to the triple bond of ethyl phenylpropiolate followed by subsequent intramolecular cyclization.
Preparation and evaluation of radiolabeled acetaminosalol microspheres: A new potential selective radiotracer for ulcerative colitis early diagnosis
Authors: El‐Kawy O. A. , Shweeta H. A. , Abdelgawad M. R. 
Published: November 2023

Acetaminosalol labeling reaction with technetium‐99m was optimized, and the radiocomplex was obtained in a high radiochemical yield of 98.9 ± 0.6% and high stability (>30 h). The tracer was characterized, and its binding to the PPARγ receptor was assessed in silico. To reduce radiation exposure to non‐target organs and increase accumulation in the colon, the tracer was formulated as pH‐sensitive microspheres with a mean particle size of 201 ± 2.1 μm, a polydispersity index of 0.18, a 25.3 ± 3.6 zeta potential, and 98.6 ± 0.33% entrapment efficiency. The system suitability was assessed in vivo in normal and ulcerative rats, and the biodistribution profile in the colon showed 56.5 ± 1.4% localization within 4 h. Blocking study suggested the selectivity of the tracer to the target receptor. Overall, the reported data encouraged the potential use of the labeled microspheres to target ulcerative colitis.
From viral democratic genomes to viral wild bunch of quasispecies
Authors: Colson Philippe , Bader Wahiba , Fantini Jacques , Dudouet Pierre , Levasseur Anthony , Pontarotti Pierre , Devaux Christian , Raoult Didier 
Published: November 2023

AbstractThe tremendous majority of RNA genomes from pathogenic viruses analyzed and deposited in databases are consensus or “democratic” genomes. They represent the genomes most frequently found in the clinical samples of patients but do not account for the huge genetic diversity of coexisting genomes, which is better described as quasispecies. A viral quasispecies is defined as the dynamic distribution of nonidentical but closely related mutants, variants, recombinant, or reassortant viral genomes. Viral quasispecies have collective behavior and dynamics and are the subject of internal interactions that comprise interference, complementation, or cooperation. In the setting of SARS‐CoV‐2 infection, intrahost SARS‐CoV‐2 genetic diversity was recently notably reported for immunocompromised, chronically infected patients, for patients treated with monoclonal antibodies targeting the viral spike protein, and for different body compartments of a single patient. A question that deserves attention is whether such diversity is generated postinfection from a clonal genome in response to selection pressure or is already present at the time of infection as a quasispecies. In the present review, we summarize the data supporting that hosts are infected by a “wild bunch” of viruses rather than by multiple virions sharing the same genome. Each virion in the “wild bunch” may have different virulence and tissue tropisms. As the number of viruses replicated during host infections is huge, a viral quasispecies at any time of infection is wide and is also influenced by host‐specific selection pressure after infection, which accounts for the difficulty in deciphering and predicting the appearance of more fit variants and the evolution of epidemics of novel RNA viruses.
Rapid and sensitive detection of 2,4‐dichlorophenoxyacetic acid residual on fruit surface via SERS‐tape sampling
Authors: Chang Min , Li Shibo , Wang Luyao , Chen Hui , Lei Ming 
Published: November 2023

AbstractThe residual 2,4‐dichlorophenoxyacetic acid (2,4‐D) in fruits poses a serious threat to human health. The continuous deposition of 2,4‐D in the body may cause symptoms such as cancer or Alzheimer's disease, therefore, it is of great significance for the detection of 2,4‐D traces on fruits. In this paper, we proposed a simple “paste‐collect‐test” strategy based on surface‐enhanced Raman spectroscopy via tape sampling (SERS‐tape sampling). The flexibility and stickiness of commercial tapes were combined with the excellent activity of optimized silver nanoparticles (Ag NPs) for rapid trace detection of pesticides in cherry tomatoes. The first two steps of “paste” and “collect” were completed by the tape to simplify the sampling procedure of the analyte molecules, and the “test” step was accomplished by deposing Ag NPs on the surface of the tape after analyte collection. The whole process was completed in a few minutes. The results showed that the limit of detection (LOD) of 2,4‐D in cherry tomatoes was 3.63 × 10−10 M with the SERS‐tape sampling approach, which is much lower than the maximum residue limit specified by the European Union (EU). This simple and easy‐to‐operate method is highly sensitive and could be a promise of 2,4‐D for food safety.
Mung bean protein as an emerging source of plant protein: a review on production methods, functional properties, modifications and its potential applications
Authors: Feng Qiqian , Niu Zhitao , Zhang Siqi , Wang Li , Qun Shen , Yan Zheng , Hou Dianzhi , Zhou Sumei 
Published: November 2023

AbstractPlant protein is rapidly becoming more of a prime interest to consumers for its nutritional and functional properties, as well as the potential to replace animal protein. In the frame of alternative protein new sources, mung bean is becoming another legume crop that could provide high quality plant protein after soybean and pea. In particular, the 8S globulins in mung bean protein have high structural similarity and homology with soybean β‐conglycinin (7S globulin), with 68% sequence identity. Currently, mung bean protein has gained popularity in food industry because of its high nutritional value and peculiar functional properties. In that regard, various modification technologies have been applied to further broaden its application. Here, we provide a review of the composition, nutritional value, production methods, functional properties and modification technologies of mung bean protein. Furthermore, its potential applications in the new plant‐based products, meat products, noodles, edible packaging films and bioactive compound carriers are highlighted to facilitate its utilization as an alternative plant protein, thus meeting consumer demands for high quality plant protein resources. © 2023 Society of Chemical Industry.
An empirical investigation of definitions of subthreshold posttraumatic stress disorder
Authors: Klein Alexandra B. , Schnurr Paula P. , Bovin Michelle J. , Friedman Matthew J. , Keane Terence M. , Marx Brian P. 
Published: November 2023

AbstractSubthreshold posttraumatic stress disorder (PTSD) has long been recognized as an important construct that identifies a subgroup of individuals who report significant PTSD symptoms and associated disability but do not endorse enough symptoms to meet the criteria for a full PTSD diagnosis. Different investigators have defined subthreshold PTSD in various ways, making it difficult to interpret findings across studies. To address this problem, we systematically compared individuals who met criteria for nine different subthreshold PTSD definitions with individuals diagnosed with either full PTSD or no PTSD (i.e., failed to meet the criteria for a subthreshold definition) with respect to prevalence and associated clinical outcomes of interest. Participants were 1,082 veterans enrolled in the Veterans After Discharge Longitudinal Registry. PTSD and subthreshold PTSD diagnostic status were determined using the Structured Clinical Interview for DSM‐5 (SCID‐5) and validated self‐report instruments were used to assess clinical outcomes. Across outcomes, subthreshold definitions generally identified a group of participants that was distinguishable from participants in both the PTSD and no PTSD groups, rs = .02–.47. We discuss the benefits and drawbacks of various subthreshold definitions and highlight the need for additional work evaluating these definitions across additional outcomes and samples. In the interim, we propose a working case definition of subthreshold PTSD as meeting any three of the four DSM‐5 symptom criteria (i.e., Criteria B, C, D, and E) along with Criterion A and Criteria F–H. The results suggest subthreshold PTSD is a clinically meaningful construct.
A practical framework for ethics assessment in wildlife management decision‐making
Authors: Smith Christian A. , Tantillo James A. , Hale Benjamin , Decker Daniel J. , Forstchen Ann B. , Pomeranz Emily F. , Lauber T. Bruce , Schiavone Michael V. , Frohlich Kipp , Lederle Patrick E. , Benedict R. Joseph , Hurst Jeremy , King Richard , Siemer William F. , Baumer Meghan S. 
Published: November 2023

AbstractWildlife professionals lack a framework and process for incorporating ethical considerations in a systematic and transparent way, along with ecological and social science, to support wildlife management decision‐making. We provide such a framework and process based on 3 of the major theoretical branches ethicists have developed in Western culture: consequentialist moral theory, which focuses on consequences and outcomes; principle‐ and rule‐based approaches that deal with what is considered right or wrong; and virtue ethical theory, which considers factors such as character, virtue, and aesthetics. The framework can be used to anticipate the ethical consequences of alternative courses of action or taking no action. If wildlife professionals use this framework as an assessment tool to provide input into decision‐making, resulting decisions will be more transparent, better understood by stakeholders, and more consistent with public trust responsibilities.
Simultaneous preconcentration of 9Be and cosmogenic 10Be for determination of the 10Be/9Be ratio in (coastal) seawater
Authors: Wang Chenyu , Frick Daniel A. , von Blanckenburg Friedhelm , Frank Martin , Lian Ergang , Yang Shouye , Wittmann Hella 
Published: November 2023

AbstractBeryllium isotopes have emerged as a quantitative tracer of continental weathering, but accurate and precise determination of the cosmogenic 10Be and stable 9Be in seawater is challenging, because seawater contains high concentrations of matrix elements but extremely low concentrations of 9Be and 10Be. In this study, we develop a new, time‐efficient procedure for the simultaneous preconcentration of 9Be and 10Be from (coastal) seawater based on the iron co‐precipitation method. The concentrations of 9Be, 10Be, and the resulting 10Be/9Be ratio for Changjiang Estuary water derived from the new procedure agree well with those obtained from the conventional procedure requiring separate preconcentration for 9Be and 10Be determinations. By avoiding the separate preconcentration, our newly developed procedure contributes toward more time‐efficient handling of samples, less sample cross‐contamination, and a more reliable 10Be/9Be ratio. Prior to this, we validated the iron co‐precipitation method using artificial seawater and natural water samples from the Amazon Estuary regarding: (1) the “matrix effect” for Be analysis, (2) its extraction efficiency for pg g−1 levels Be in the presence and absence of organic matter, and (3) the data comparability with another preconcentration method. We calculated that for the determination of 9Be and 10Be in most open ocean seawater with typical 10Be concentrations of > 500 atoms g−1, good precisions (< 5%) can be achieved using less than 3 liters of seawater compared to more than 20 liters routinely used previously. Even for coastal seawater with extremely low 10Be concentration (e.g., 100 atoms g−1), we estimate a maximum amount of 10 liters to be adequate.