bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of hypoxia and oxidative-related changes in a lung-derived brain metastasis model by [64Cu][Cu(ATSM)] PET and proteomic studies
Authors: Fantin Jade , Toutain Jérôme , Pérès Elodie A. , Bernay Benoit , Mehani Sarina Maya , Helaine Charly , Bourgeois Mickael , Brunaud Carole , Chazalviel Laurent , Pontin Julien , Corroyer-Dulmont Aurélien , Valable Samuel , Cherel Michel , Bernaudin Myriam 
Published: November 2023

Abstract
Background
Brain metastases (BM) are the most frequent malignant brain tumors. The aim of this study was to characterize the tumor microenvironment (TME) of BM and particularly hypoxia and redox state, known to play a role in tumor growth and treatment resistance with multimodal PET and MRI imaging, immunohistochemical and proteomic approaches in a human lung cancer (H2030-BrM3)-derived BM model in rats.

Results
First, in vitro studies confirmed that H2030-BrM3 cells respond to hypoxia with increasing expression of HIF-1, HIF-2 and their target genes. Proteomic analyses revealed, among expression changes, proteins associated with metabolism, oxidative stress, metal response and hypoxia signaling in particular in cortical BM. [64Cu][Cu(ATSM)] PET revealed a significant uptake by cortical BM (p < 0.01), while no uptake is observed in striatal BM 23 days after tumor implantation. Pimonidazole, HIF-1α, HIF-2α, CA-IX as well as GFAP, CTR1 and DMT1 immunostainings are positive in both BM.

Conclusion
Overall, [64Cu][Cu(ATSM)] imaging and proteomic results showed the presence of hypoxia and protein expression changes linked to hypoxia and oxidative stress in BM, which are more pronounced in cortical BM compared to striatal BM. Moreover, it emphasized the interest of [64Cu][Cu(ATSM)] PET to characterize TME of BM and depict inter-metastasis heterogeneity that could be useful to guide treatments.
Volatile Kernel Rootkit hidden process detection in cloud computing
Authors: S Suresh Kumar , T Sudalai Muthu 
Published: November 2023

AbstractThe rootkit industry has advanced significantly in the last decade. Attackers want to leave a backdoor for quick reoccurring exploits rather than launching the traditional one-time worm/virus attacks. Meanwhile, as intrusion detection technologies improve, rootkits have grown in popularity. For the attackers to succeed, stealth becomes critical. The primary function of rootkits is to provide stealth. The modifications a rootkit makes conceal the presence of a rootkit. Determining the presence of mutation rootkits was quite challenging. Attackers can silently alter volatile (processes) and non-volatile (files) with the aid of rootkits without being noticed. We suggested the VKRHPDV (Volatile Kernel Rootkit Hidden Process Detection) framework to find the hidden techniques. This system includes process monitors, process comparison analysts, and contaminated process data gathering. Process monitoring is nothing more than clean process collection in the absence of rootkits, whereas pure process collection has been corrupted by rootkit injection. The process analyzer compares clean and tainted processes, some of which were concealed. VKRHPDV can identify process hiding behaviors in all datasets in the shortest period, according to the findings of an extensive performance analysis carried out on 64 rootkit datasets for each UNIX and Windows kernel in a cloud environment.
The impact of entrepreneurship knowledge on students' e-entrepreneurial intention formation and the moderating role of technological innovativeness
Authors: Pham Minh , Nguyen Anh Tuyet Thi , Tran Duy Thien , Mai Tam Toai , Nguyen Vinh Trung 
Published: November 2023

AbstractIn the era of Industry 4.0, online entrepreneurship is gradually seen as an essential tool that brings innovative values to the integration and development of a country. The rapid failure of nascent businesses stemming from a lack of human capital directed this study to explore the role of students' knowledge and technological innovativeness in motivating students to form e-entrepreneurial intentions. Information collected from 405 students from universities in Vietnam via online questionnaires was analysed using SmartPLS 4 to test the relationships among seven factors proposed in the research model. The results show that entrepreneurial education and prior experiences are the foundation for improving students' perceived feasibility and usefulness, forming their entrepreneurial motivation. Next, entrepreneurial motivation becomes an important premise for promoting students' entrepreneurial intentions more effectively. Furthermore, the study found that students' technological innovativeness moderated the relationship between their entrepreneurial motivation and intention to set up a digital business.
Metagenome-assembled genomes reveal greatly expanded taxonomic and functional diversification of the abundant marine Roseobacter RCA cluster
Authors: Liu Yanting , Brinkhoff Thorsten , Berger Martine , Poehlein Anja , Voget Sonja , Paoli Lucas , Sunagawa Shinichi , Amann Rudolf , Simon Meinhard 
Published: November 2023

Abstract
Background
The RCA (Roseobacter clade affiliated) cluster belongs to the family Roseobacteracea and represents a major Roseobacter lineage in temperate to polar oceans. Despite its prevalence and abundance, only a few genomes and one described species, Planktomarina temperata, exist. To gain more insights into our limited understanding of this cluster and its taxonomic and functional diversity and biogeography, we screened metagenomic datasets from the global oceans and reconstructed metagenome-assembled genomes (MAG) affiliated to this cluster.

Results
The total of 82 MAGs, plus five genomes of isolates, reveal an unexpected diversity and novel insights into the genomic features, the functional diversity, and greatly refined biogeographic patterns of the RCA cluster. This cluster is subdivided into three genera: Planktomarina, Pseudoplanktomarina, and the most deeply branching Candidatus Paraplanktomarina. Six of the eight Planktomarina species have larger genome sizes (2.44–3.12 Mbp) and higher G + C contents (46.36–53.70%) than the four Pseudoplanktomarina species (2.26–2.72 Mbp, 42.22–43.72 G + C%). Cand. Paraplanktomarina is represented only by one species with a genome size of 2.40 Mbp and a G + C content of 45.85%. Three novel species of the genera Planktomarina and Pseudoplanktomarina are validly described according to the SeqCode nomenclature for prokaryotic genomes. Aerobic anoxygenic photosynthesis (AAP) is encoded in three Planktomarina species. Unexpectedly, proteorhodopsin (PR) is encoded in the other Planktomarina and all Pseudoplanktomarina species, suggesting that this light-driven proton pump is the most important mode of acquiring complementary energy of the RCA cluster. The Pseudoplanktomarina species exhibit differences in functional traits compared to Planktomarina species and adaptations to more resource-limited conditions. An assessment of the global biogeography of the different species greatly expands the range of occurrence and shows that the different species exhibit distinct biogeographic patterns. They partially reflect the genomic features of the species.

Conclusions
Our detailed MAG-based analyses shed new light on the diversification, environmental adaptation, and global biogeography of a major lineage of pelagic bacteria. The taxonomic delineation and validation by the SeqCode nomenclature of prominent genera and species of the RCA cluster may be a promising way for a refined taxonomic identification of major prokaryotic lineages and sublineages in marine and other prokaryotic communities assessed by metagenomics approaches.
Clec7a drives gut fungus-mediated host lipid deposition
Authors: Ma Jie , Zhou Miao , Song Zehe , Deng Yuankun , Xia Siting , Li Yunxia , Huang Xingguo , Xiao Dingfu , Yin Yulong , Yin Jie 
Published: November 2023

Abstract
Background
Compared to that of bacteria, the role of gut fungi in obesity development remains unknown.

Results
Here, alterations in gut fungal biodiversity and composition were confirmed in obese pig models and high-fat diet (HFD)-fed mice. Antifungal drugs improved diet-induced obesity, while fungal reconstruction by cohousing or fecal microbiota transplantation maintained the obese phenotype in HFD-fed mice. Fungal profiling identified 5 fungal species associated with obesity. Specifically, Ascomycota_sp. and Microascaceae_sp. were reduced in obese mice and negatively correlated with fat content. Oral supplementation with fungi was sufficient to prevent and treat diet-induced obesity. Clec7a, which is involved in fungal recognition, was highly expressed in HFD-fed mice. The Clec7a agonist accelerated diet-induced obesity, while Clec7a deficieny in mice resulted in resistance to diet-induced obesity and blocked the anti-obese effect of antifungal drugs and fungi.

Conclusions
Taken together, these results indicate that gut fungi/Clec7a signaling is involved in diet-induced obesity and may have therapeutic implications as a biomarker for metabolic dysregulation in humans.
Gut microbiota-bile acid crosstalk regulates murine lipid metabolism via the intestinal FXR-FGF19 axis in diet-induced humanized dyslipidemia
Authors: Xu Hongtao , Fang Fang , Wu Kaizhang , Song Jiangping , Li Yaqian , Lu Xingyu , Liu Juncheng , Zhou Liuyang , Yu Wenqing , Yu Fei , Gao Jie 
Published: November 2023

Abstract
Background
Diet-induced dyslipidemia is linked to the gut microbiota, but the causality of microbiota-host interaction affecting lipid metabolism remains controversial. Here, the humanized dyslipidemia mice model was successfully built by using fecal microbiota transplantation from dyslipidemic donors (FMT-dd) to study the causal role of gut microbiota in diet-induced dyslipidemia.

Results
We demonstrated that FMT-dd reshaped the gut microbiota of mice by increasing Faecalibaculum and Ruminococcaceae UCG-010, which then elevated serum cholicacid (CA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA), reduced bile acid synthesis and increased cholesterol accumulation via the hepatic farnesoid X receptor-small heterodimer partner (FXR-SHP) axis. Nevertheless, high-fat diet led to decreased Muribaculum in the humanized dyslipidemia mice induced by FMT-dd, which resulted in reduced intestinal hyodeoxycholic acid (HDCA), raised bile acid synthesis and increased lipid absorption via the intestinal farnesoid X receptor-fibroblast growth factor 19 (FXR-FGF19) axis.

Conclusions
Our studies implicated that intestinal FXR is responsible for the regulation of lipid metabolism in diet-induced dyslipidemia mediated by gut microbiota-bile acid crosstalk.
In-silico and in-vitro screening of Asiatic acid and Asiaticoside A against Cathepsin S enzyme
Authors: Ajani Temitope Akinwumi , Obikeze Kenechukwu , Magwebu Zandisiwe E. , Egieyeh Samuel , Chauke Chesa G. 
Published: November 2023

Abstract
Background
Atherosclerosis is a form of cardiovascular disease that affects the endothelium of the blood vessel. Series of events are involved in the pathophysiology of this disease which includes the breaking down of the connective tissue elastin and collagen responsible for the tensile strength of the arterial wall by proteolytic enzyme. One of these enzymes called Cathepsin S (CatS) is upregulated in the progression of the disease and its inhibition has been proposed to be a promising pharmacological target to improve the prognosis of the disease condition. Asiatic acid and asiaticoside A are both pentacyclic triterpenoids isolated from Centella asiatica. Their use in treating various cardiovascular diseases has been reported.

Methods
In this study through in silico and in vitro methods, the pharmacokinetic properties, residue interaction, and inhibitory activities of these compounds were checked against the CatS enzyme. The SwissADME online package and the ToxTree 3.01 version of the offline software were used to determine the physicochemical properties of the compounds.

Result
Asiatic acid reported no violation of the Lipinski rule while asiaticoside A violated the rule with regards to its molecular structure and size. The molecular docking was done using Molecular Operating Environment (MOE) and the S-score of − 7.25988, − 7.08466, and − 4.147913 Kcal/mol were recorded for LY300328, asiaticoside A, and asiatic acid respectively. Asiaticoside A has a docking score value (− 7.08466Kcal/mol) close to the co-crystallize compound. Apart from the close docking score, the amino acid residue glycine69 and asparagine163 both interact with the co-crystallized compound and asiaticoside A. The in vitro result clearly shows the inhibitory effect of asiaticoside and asiatic acid. Asiaticoside A has an inhibitory value of about 40% and asiatic acid has an inhibitory value of about 20%.

Conclusion
This clearly shows that asiaticoside will be a better drug candidate than asiatic acid in inhibiting the CatS enzyme for the purpose of improving the outcome of atherosclerosis. However, certain modifications need to be made to the structural make-up of asiaticoside A to improve its pharmacokinetics properties.
What does my appearance have to do with my job? Vocational students on the sexual harassment from guests in the hospitality industry
Authors: Hedlin Maria , Klope Eva 
Published: November 2023

AbstractSexual harassment by guests is part of the vocational culture in many workplaces within the hospitality industry. In this study, we investigate vocational students' descriptions of sexual harassment by guests in the hospitality industry and how these instances can be understood in terms of objectification. The empirical material consists of focus group interviews with upper secondary school students who are training to work in the hospitality industry. The students describe four categories of sexual harassment: appearance comments, private and intrusive questions, leering, and physical contact. In the descriptions, men are sexually harassing female students or employees. The types of objectification in the descriptions are reduction to appearance, reduction to body, instrumental approach, denial of autonomy, denial of bodily integrity, and denial of subjectivity. Regardless of the type of objectification involved, a dilemma arises for the girls who have been taught that being courteous to guests is part of the professional role, while the guests are treating them as sexual objects.
Biomechanical evaluation of the spring ligament and the posterior tibial tendon by shear‐waves elastography: validation of a reliable and reproducible measurement protocol
Authors: Rougereau Grégoire , Marty‐Diloy Thibault , Vigan Marie , Donadieu Kalinka , Vialle Raphaël , Langlais Tristan , Hardy Alexandre 
Published: November 2023

AbstractPurposeThe anatomy of the spring ligament complex, as well as its pathology, is not well known in daily clinical practice. The purpose of this study was to evaluate the shear‐wave elastography properties of the spring ligament and the posterior tibial tendon in healthy adults, and to assess the reliability and reproducibility of these measurements.MethodsShear‐wave elastography was used to evaluate both ankles in 20 healthy patients (10 females/10 males) resting on a hinge support with their ankles in neutral, valgus 20° and varus 30° positions. The stiffness of the spring ligament and posterior tibial tendon was assessed by measuring the speed of shear wave propagation through each structure.ResultsPosterior tibial tendon and spring ligament reach a maximum estimated stiffness in valgus 20° position (7.43 m/s vs 5.73 m/s, respectively). Flat feet were associated with greater spring ligament stiffness in the 20° valgus position (p = 0.01), but not for the posterior tibial tendon (p = 0.71). The physiologic weightbearing hindfoot attitude had no impact on the stiffness of the posterior tibial tendon or the spring ligament, regardless of the analysis position. Intra‐ and inter‐observer agreements were all excellent for spring ligament stiffness, regardless of ankle position, and were good or excellent for posterior tibial tendon.ConclusionsThis study describes a protocol to assess the stiffness of tibialis posterior and the spring ligament by shear‐wave elastography, which is reliable, reproducible, and defines a corridor of normality. Further studies should be conducted to define the role of elastography for diagnosis/ evaluation of pathology, follow‐up, or surgical strategies.
Targeting GPR65 alleviates hepatic inflammation and fibrosis by suppressing the JNK and NF-κB pathways
Authors: Zhang Kun , Zhang Meng-Xia , Meng Xiao-Xiang , Zhu Jing , Wang Jia-Jun , He Yi-Fan , Li Ye-Hua , Zhao Si-Cong , Shi Zhe-Min , Zheng Li-Na , Han Tao , Hong Wei 
Published: November 2023

Abstract
Background
G-protein coupled receptors (GPCRs) are recognized as attractive targets for drug therapy. However, it remains poorly understood how GPCRs, except for a few chemokine receptors, regulate the progression of liver fibrosis. Here, we aimed to reveal the role of GPR65, a proton-sensing receptor, in liver fibrosis and to elucidate the underlying mechanism.

Methods
The expression level of GPR65 was evaluated in both human and mouse fibrotic livers. Furthermore, Gpr65-deficient mice were treated with either bile duct ligation (BDL) for 21 d or carbon tetrachloride (CCl4) for 8 weeks to investigate the role of GPR65 in liver fibrosis. A combination of experimental approaches, including Western blotting, quantitative real-time reverse transcription‑polymerase chain reaction (qRT-PCR), and enzyme-linked immunosorbent assay (ELISA), confocal microscopy and rescue studies, were used to explore the underlying mechanisms of GPR65’s action in liver fibrosis. Additionally, the therapeutic potential of GPR65 inhibitor in the development of liver fibrosis was investigated.

Results
We found that hepatic macrophages (HMs)-enriched GPR65 was upregulated in both human and mouse fibrotic livers. Moreover, knockout of Gpr65 significantly alleviated BDL- and CCl4-induced liver inflammation, injury and fibrosis in vivo, and mouse bone marrow transplantation (BMT) experiments further demonstrated that the protective effect of Gpr65 knockout is primarily mediated by bone marrow-derived macrophages (BMMs). Additionally, in vitro data demonstrated that Gpr65 silencing and GPR65 antagonist inhibited, while GPR65 overexpression and application of GPR65 endogenous and exogenous agonists enhanced the expression and release of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and transforming growth factor-β (TGF-β), all of which subsequently promoted the activation of hepatic stellate cells (HSCs) and the damage of hepatocytes (HCs). Mechanistically, GPR65 overexpression, the acidic pH and GPR65 exogenous agonist induced up-regulation of TNF-α and IL-6 via the Gαq-Ca2+-JNK/NF-κB pathways, while promoted the expression of TGF-β through the Gαq-Ca2+-MLK3-MKK7-JNK pathway. Notably, pharmacological GPR65 inhibition retarded the development of inflammation, HCs injury and fibrosis in vivo.

Conclusions
GPR65 is a major regulator that modulates the progression of liver fibrosis. Thus, targeting GPR65 could be an effective therapeutic strategy for the prevention of liver fibrosis.
Immune thrombocytopenic purpura after influenza vaccine administration; a systematic review and meta-analysis
Authors: Elsaid Mohamed , Nune Arvind , Brakat Aml M. , Anand Ayush , Alashwah Mahmoud , Maher Ahmed , Lama Nitu , Peñamante Criselle Angeline C. 
Published: November 2023

Abstract
Background
The American Society of Haematology defines immune thrombocytopenic purpura (ITP) as a common hematologic disorder characterized by a transient or long-term decrease in platelet counts (< 100 × 109/L.), purpura, and haemorrhagic episodes caused by antiplatelet autoantibodies, with the exclusion of other clinical conditions. We aimed to systematically determine the incidence of ITP in adults and children following influenza vaccination, the duration between vaccination and the occurrence of ITP, and to identify predictors of ITP after the vaccine.

Methods
We searched PubMed, Cochrane Library, Google Scholar, Web of Science, Scopus, and Science Direct. We included primary studies that assessed the occurrence of immune thrombocytopenia in individuals who had received any influenza vaccine (primary or booster dose), regardless of the dosage, preparation, time of administration, or age of the participants. We excluded studies that were (a) Narrative, scoping, and umbrella reviews ;(b) studies with no accessible full text, abstract-only studies, or (c) Overlapping or unreliable data. The risk of bias in the included studies was assessed using the Joanna Briggs Institute (JBI) tool. We categorized studies for qualitative analysis based on study design. Descriptive statistics were used to summarize quantitative data, including the incidence of ITP after influenza vaccination.

Results
Out of 729 articles retrieved from the database search, we included 24 studies. All patients identified and included in this systematic review presented with immune thrombocytopenia, determined by their platelet count. The period between vaccination and the occurrence of ITP ranged from (2:35 days). The mean duration was 13.5 days. The analysis revealed a statistically significant incidence rate ratio (IRR) = 1.85,95% CI [1.03–3.32] of ITP occurrence after 42 days.

Conclusions
Influenza-associated ITP is uncommon, self-limiting, non-life-threatening, and curable. None of the patients reported having severe adverse events or death. Further studies are required to confirm the exact incidence of the ITP to better understand the pathophysiology of ITP development post-influenza vaccination.
Three Cases of Carney-Stratakis Syndrome: A Genetically Heterogeneous Disease
Authors: Lobato Eduardo C , Castro Felipe F , Santana Lucas S , Soares Ibere C , Fagundes Gustavo F C , Almeida Madson Q 
Published: November 2023

Abstract
Carney-Stratakis syndrome (CSS) is an autosomal dominant rare syndrome, with incomplete penetrance, characterized by the association of paragangliomas and/or pheochromocytomas and gastrointestinal stromal tumors (GISTs). CSS is caused by germline heterozygous loss-of-function pathogenic variants (PVs) in the succinate dehydrogenase subunit genes (SDHB, SDHC, SDHD), with SDHB and SDHD being the most frequent. To date, only 2 germline SDHC PVs (c.43 C &gt; T; c.405 + 1G &gt; A) have been described in 3 patients with CSS. Three patients with CSS and very distinct clinical presentations are reported here: 1 caused by a germline SDHC large deletion and the others with metastatic GIST and negative genetic investigation for SDHx defects. Two cases (1 and 2) presented with pheochromocytoma (case 1 also with abdominal paraganglioma) and metastatic GIST. Although these 2 cases fulfilled the diagnostic criteria for CSS, the genetic investigation for SDHx PVs by next-generation sequencing and multiplex ligation-dependent probe amplification was negative. Case 3 had a large abdominal paraganglioma and a small low-grade GIST not associated with recurrence or metastasis. This case harbored a germline SDHC exon 3 deletion, not previously reported. In conclusion, CSS is a rare and morbid disease with distinct clinical presentations and genetic heterogeneity, which can contribute to underdiagnosis.