bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Parameter Estimates for Carcass and Meat Quality Traits and Their Genetic Associations With Sexual Precocity Indicator Traits in Nellore Cattle
Authors: Arikawa Leonardo Machestropa , Mota Lucio Flavio Macedo , Schmidt Patrícia Iana , Salatta Bruna Maria , Nasner Sindy Liliana Caivio , Silva Neto João Barbosa da , Fonseca Larissa Fernanda Simielli , Magalhães Ana Fabrícia Braga , Silva Delvan Alves , Carvalheiro Roberto , Chardulo Luis Artur Loyola , de Albuquerque Lucia Galvão 
Published: February 2025

ABSTRACTFor developing beef cattle breeding programmes, it is essential to understand the genetic basis of economically relevant traits, such as carcass, meat quality and female sexual precocity. However, the direct selection of most of these traits is a challenge for producers because of the high cost and measurement difficulty. Genetic correlation estimates between carcass and meat quality traits obtained after slaughter and sexual precocity indicator traits in Nellore are limited in the literature. Thus, this study aimed to estimate genetic parameters for longissimus muscle area (LMA), backfat thickness (BF), hot carcass weight (HCW), shear force tenderness (SF), marbling score (MARB), intramuscular fat content (IMF), age at first calving (AFC), heifer pregnancy (HP) and scrotal circumference (SC) in Nellore cattle, using pedigree and genomic information. For this, data from 6910 young bulls with phenotypic information for carcass and meat traits, 230,682 for sexual precocity indicator traits, and 17,850 animals genotyped with or imputed to the Illumina Bovine HD BeadChip were used. The (co)variance components and genetic parameters were estimated considering BLUP and single‐step GBLUP (ssGBLUP) models via Bayesian inference using the GIBBSF90+ software. The multi‐trait animal model included additive and residual genetic effects as random; the fixed effects of contemporary group (for all traits) and date of analysis as classes (for BF, SF and MARB); and the linear effects of age at slaughter (all carcass and meat traits) and age at yearling (YW and SC) as covariates. Heritability estimates ranged from 0.13 to 0.34 for carcass and meat quality traits, and for SC, AFC and HP, were 0.33, 0.07 and 0.29, respectively. Favourable genetic correlations were estimated between YW‐HCW (0.79 ± 0.03), YW‐LMA (0.28 ± 0.05), YW‐SC (0.35 ± 0.03), HCW‐LMA (0.44 ± 0.05), HCW‐SF (−0.22 ± 0.09), HCW‐SC (0.19 ± 0.05), MARB‐IMF (0.90 ± 0.07), SF‐IMF (−0.20 ± 0.11), BF‐MARB (0.29 ± 0.08), BF‐IMF (0.22 ± 0.09), BF‐AFC (−0.21 ± 0.07) and BF‐HP (0.24 ± 0.10). In general, the correlations between carcass traits and those of meat quality were low to moderate. Additionally, carcass and meat quality traits did not exhibit strong genetic correlations with female precocity indicators. So, to achieve significant genetic advances in female sexual indicator traits, carcass composition and meat quality, these traits must compose selection indices for Nellore cattle.
The Relationship Between Long Covid Symptoms and Vaccination Status in COVID‐19 Survivors
Authors: Öztürk Furkan , Emiroğlu Canan , Aypak Cenk 
Published: February 2025

ABSTRACTBackgroundThe positive effects of vaccination status on the course of Long COVID symptoms have not been fully elucidated. Our aim is to determine the most common Long COVID symptoms in patients monitored in the COVID‐19 follow‐up clinic and to examine whether there is a difference between the recovery rates of those who are vaccinated and those who are not vaccinated.MethodBetween December 1, 2020 and April 30, 2022, prospectively collected data of 916 patients who were admitted to the COVID‐19 follow‐up outpatient clinic of a tertiary hospital for the first time were evaluated as a retrospective cohort in this study. The frequencies of the ten most common symptoms in the first and last examinations of 478 patients with Long COVID symptoms were determined, and their recovery was compared. Patients were divided into two groups according to their vaccination status. The values showing the recovery rates obtained for these two groups were compared between themselves again.ResultsThe average age of the patients in the study group was 54.43 ± 11.71 years, and 255 (53.3%) were male. The median follow‐up period was 10 months. 84.7% of patients had received at least one dose of vaccine. Statistically significant results were found for improvement in all ten symptoms in vaccinated patients compared to the never‐vaccinated group. There was no statistically significant difference between the CoronaVac, BNT162b2, and heterologous (CoronaVac+ BNT162b2) vaccine groups. Factors affecting recovery for the three most common symptoms (dyspnea, fatigue, forgetfulness) were examined with univariate logistic regression analysis, and only vaccination or non‐vaccination was found to be a significant risk factor.ConclusionThis study showed that receiving vaccination may be effective in improving Long COVID symptoms. Although there were no statistically significant differences between the inactive vaccine CoronaVac, the mRNA vaccine BNT162b2, and the heterologous (CoronaVac+ BNT162b2) vaccine in terms of reducing Long COVID symptoms, higher recovery rates were detected in those who received the mRNA vaccine BNT162b2.
Parents' Medicine‐Seeking Behavior and Their Beliefs About the Efficacy of Medicines
Authors: Dawood Omar Thanoon , Al‐Shammaa Zainab M. 
Published: February 2025

ABSTRACTObjectivesParents' medicine‐seeking behavior is often influenced by their own experiences with illness. The purpose of this study was to investigate parents' medicine‐seeking behavior and their beliefs regarding the efficacy of medicines.MethodsA cross‐sectional study was conducted among parents in Mosul, Iraq. A convenience sampling method was used to select the parents who visited community pharmacies to buy medication for their ill children. A self‐administered questionnaire was utilized to collect data from 380 parents who successfully completed the survey.ResultsThe majority of parents (83.4%) stated that they read the labels of medications before giving them to their children, and more than half of the parents (57.9%) did not buy medicines for their children without consulting a doctor. In addition, 65% of the participants asked about the possible side effects of the medicines. Additionally, the majority of parents (73.2%) believed that branded medicines were more effective than generic medicines, and 63.4% of them believed that the efficacy of medicines is not related to the manufacturing countries. Furthermore, 62.9% of the parents believed that the efficacy of medicines is not related to their price, while 35.2% of them believed that injections were more effective than other dosage forms. Parents' medicine‐seeking behavior and their beliefs about the efficacy of medicines were significantly associated with parents' higher education level and higher family income.ConclusionThere was inadequate information among parents concerning the use of medicines, including side effects, proper utilization, and the importance of seeking medical assistance. Furthermore, parents have false beliefs about the efficacy of medicines.
Influence of Hydrogen Introduced Drying Atmosphere on Drying Kinetics, Phenolic Profile, and Rehydration Behavior of Tomato Slices
Authors: Perumkulam Lakshmanan Meenakshi , Gopal Amuthaselvi , Perumal Rajkumar , Gurumurthy Gurumeenakshi , Manickam Anand , Murugesan Balakrishnan , Jaganathan Deepa , Sivamani Ganga Kishore , Durgawati  , Murugesan Arulkumar 
Published: February 2025

ABSTRACTThe current study performed drying of tomato slices in a drying atmosphere with hydrogen gas (RADMIX; a gaseous mixture of 4% hydrogen, 5% carbon dioxide and 91% nitrogen), and compared with 100% air, 100% nitrogen drying environments. All the drying experiments were carried out at 60°C. Control samples and pretreated samples with 1% Potassium metabisulfite (KMS) treated samples were dried to a final moisture content of around 13.29% to 13.70% (wet basis), respectively. The drying behavior and quality characteristics including color change (ΔE), lycopene retention, total phenolics and rehydration ratio of the dried products were studied. The better retention of color and quality was observed in the sulfite pretreated and RADMIX dried sample. The color change (ΔE) was found to be 10.1, lycopene retention by 94.28% (3.3 mg/100 g), and the maximum rehydration ratio of 4.67 were observed in samples subjected to reduced atmospheric drying. Additionally, the color change was lesser for samples pretreated with KMS than the control samples. It was found that the use of hydrogen gas at 4% concentration (RADMIX) in the drying environment significantly impacted quality parameters of tomato slices. The sulfite pretreatment was advantageous technique in terms of moisture diffusivity, antioxidant compounds retention and rehydration ratio of tomato slices.
The Impact of Home Enteral Tube‐Feeding on the Intent and Experience of Going Out in Public: A Qualitative Study on Patients' and Caregivers' Perspectives
Authors: Lee Yan Shan , Zulkifli Nafisah , Lim Yi Huan , Lim Shinee Ying Yi , Chow Benjamin Jian Wen , Salazar Ennaliza , Wong Seng Mun 
Published: February 2025

ABSTRACTBackgroundIndividuals on tube‐feeding experience significant lifestyle changes and social isolation, but the barriers and support for travelling out while on tube‐feeding to reintegrate into the community have not been studied. This research explores the factors influencing the decisions of individuals on tube‐feeding and their carers to travel outside of their homes, their experiences, and perceived barriers and support when performing tube‐feeding in public settings.MethodsA qualitative design using purposive sampling explored the experiences of three individuals and five carers living at home on long‐term tube‐feeding. One‐to‐one semi‐structured interviews were conducted and transcribed. Thematic analysis was conducted via open coding and merging of recurring codes to form themes.ResultsSix themes emerged: (1) Motivators and deterrents to going out: Motivators include desire for social normalcy. Deterrents include physical health, logistics and psychosocial considerations, such as fear of negative public perception and the loss of travelling and dining as motivating social activities. (2) Going out requires meticulous preparation and planning. (3) Inadequate tube‐feeding facilities in the community contribute to ambivalence and discomfort to tube‐feeding outside. Participants must accept potential negative emotions when confronted with public reaction to tube‐feeding. (4) Ideal tube‐feeding facilities should be private and hygienic, in highly frequented locations. (5) Community support and awareness are needed to support tube‐feeding. (6) Adaptability is crucial when satisfactory feeding locations are absent during local and overseas travel.ConclusionsCarers and healthcare professionals are pivotal in encouraging patients to disregard public curiosity, planning tube‐feeding logistics, and advocating for the right to tube feed in public spaces without shame. Public awareness, carer support, and availability of tube‐feeding facilities should be improved for patients to live, work and engage meaningfully in the community.
Focused Ultrasound Modulates Dopamine in a Mesolimbic Reward Circuit
Authors: Olaitan Greatness , Ganesana Mallikarjunarao , Strohman Andrew , Lynch Wendy J. , Legon Wynn , Venton B. Jill 
Published: February 2025

ABSTRACTDopamine is a neurotransmitter that plays a significant role in reward and motivation. Dysfunction in the mesolimbic dopamine pathway has been linked to a variety of psychiatric disorders, including addiction. Low‐intensity focused ultrasound (LIFU) has demonstrated effects on brain activity, but how LIFU affects dopamine neurotransmission is not known. Here, we applied three different intensities (6.5, 13, and 26 W/cm2 ISPPA) of 2‐min LIFU to the prelimbic cortex (PLC) and measured dopamine in the nucleus accumbens (NAc) core using fast‐scan cyclic voltammetry. Two minutes of LIFU sonication at 13 W/cm2 to the PLC significantly reduced dopamine release by ~50% for up to 2 h. However, double the intensity (26 W/cm2) resulted in less inhibition (~30%), and half the intensity (6.5 W/cm2) did not result in any inhibition of dopamine. Anatomical controls applying LIFU to the primary somatosensory cortex did not change NAc core dopamine, and applying LIFU to the PLC did not affect dopamine release in the caudate or NAc shell. Histological evaluations showed no evidence of cell damage or death. Modeling temperature rise demonstrates a maximum temperature change of 0.5°C with 13 W/cm2, suggesting that modulation is not due to thermal mechanisms. These studies show that LIFU at a moderate intensity provides a noninvasive, high spatial resolution means to modulate specific mesolimbic circuits that could be used in future studies to target and repair pathways that are dysfunctional in addiction and other psychiatric diseases.image
FOXP1 is a Transcription Factor for the Alzheimer's Disease Risk Gene SORL1
Authors: Fu Xiaofeng , Quan Shuiyue , Liang Wenping , Li Yu , Cai Huimin , Ren Ziye , Xu Yinghao , Wang Zhe , Jia Longfei 
Published: February 2025

ABSTRACTSortilin‐related receptor 1 (SORL1) is a risk gene of Alzheimer's disease (AD), and some protein‐truncating (PTV) and rare missense variants causing the loss of function of SORL1 contribute to AD pathogenesis. SORL1 is an endosomal receptor that interacts with multiple protein sorting complexes to facilitate the transport of various cargoes through the endolysosomal network (ELN). However, the regulatory mechanisms governing SORL1 expression remain unknown. Through biochemical methods, we identified Forkhead Box P1 (FOXP1) as a binding protein to the minimal promoter region of SORL1 gene. Silencing FOXP1 using siRNA significantly decreased the activity of the SORL1 minimal promoter and reduced SORL1 protein and mRNA levels in the neuroblastoma cell line SH‐SY5Y. Additionally, using 5xFAD mouse models of AD, we observed significantly decreased FOXP1 and SORL1 expression in neurons within the prefrontal cortex. Disruption of ELN and the autophagy degradation system by bafilomycin A1 (BafA1) appeared to be a specific condition to suppress FOXP1 and hence SORL1 in SH‐SY5Y cells. These findings highlight the critical role of FOXP1 in regulating SORL1 expression and suggest that FOXP1 could be a potential target to maintain SORL1 expression for AD prevention and therapy.image
Women who initiate divorce embrace autonomy
Authors: Almog Ephrat , Herbst‐Debby Anat 
Published: February 2025

AbstractObjectiveThis study investigates the coping mechanisms and resilience of women who initiate divorce, with a focus on their development of autonomy post‐divorce.BackgroundLife after divorce is viewed as a transition period with new opportunities. This is particularly pertinent in Israel, a familistic, traditional society where family and marriage are centrally controlled by religious institutions.MethodA qualitative methodology involved semi‐structured in‐depth interviews with 38 Israeli‐Jewish mothers (ages 25–70) who initiated divorce. Data analysis included identifying central motifs, creating a category tree, and using the Narrlarizer software to organize data.ResultsWomen described their challenging roles re‐independence and control within their families, with their narratives highlighting their shift towards becoming family leaders and perceiving themselves as experiencing greater autonomy.ConclusionWomen who initiate divorce act against societal norms, breaking through marital barriers to gain autonomy and control over their lives. This aligns with authentic behavior, emphasizing their strength and the reclamation of personal agency.ImplicationsOur research provides a nuanced perspective on women initiating divorce, recognizing their liberation from patriarchal norms and gains despite diverse struggles. It underscores opportunities for reshaping women's societal roles and self‐identity, presenting divorce initiation as an act of autonomy and resistance to gender oppression, and enriching discussions on women's experiences.
Maternal Knowledge and Care Ethics in Navigating the Stances on Abortion Taken by Young Catholic Mothers in Poland
Authors: Krotofil Joanna , Wójciak Dorota , Mętel Dagmara 
Published: February 2025

ABSTRACTIn this paper, we explore young Polish Catholic mothers' moral reasoning on abortion. We draw on the concept of maternal knowledge and theoretical insights developed within the ethics of care to shed light on the complexities and contradictions experienced by Catholic mothers in the context of reproductive choices. The narratives we gathered through in‐depth interviews illustrate how mothers evoke embodied, experience‐based maternal knowledge to challenge hegemonic frameworks associated with legal, medical, and religious authoritative knowledges. We show how mothers engage critically with ideologized notions of sacrifice and suffering by placing these concepts in the context of relational maternal care work and argue that mothers adopt a position on the moral permissibility of abortion by reinterpreting religious norms and ideas, such as the protection of the family and vocation of care.
Neuropsychiatric Side Effects of Hydroxychloroquine in a Patient With Idiopathic Pulmonary Hemosiderosis
Authors: Cengiz Didar Ağca , Başaran Abdurrahman Erdem , Parlak Betül Bankoğlu , Şambel Irmak Tanal , Bingöl Ayşen 
Published: February 2025

ABSTRACTBackgroundIdiopathic pulmonary hemosiderosis (IPH) is a rare interstitial lung disease. Glucocorticosteroids and hydroxychloroquine are the most commonly used treatments. Although neuropsychiatric side effects related to hydroxychloroquine use are seen in adult cases, only one paediatric patient has been reported in the literature.Case PresentationWe report a case of a 6‐year‐old girl with IPH, who developed neuropsychiatric symptoms, including restlessness, confusion and myoclonic movements, after the therapeutic use of hydroxychloroquine.ConclusionWith increasing knowledge and experience of interstitial lung disease, the use of hydroxychloroquine treatment is increasing. It is important to remember that hydroxychloroquine is a central nervous system stimulant, and neuropsychiatric side effects may be seen in children. This report highlights the importance of recognising potential neuropsychiatric side effects in paediatric patients using hydroxychloroquine, especially when combined with corticosteroids or other risk factors.
CAS9 Mediated In‐Planta Defence Strategy Against Tomato Leaf Curl New Delhi Virus (ToLCNDV) in Tomato
Authors: Ali Muhammad Ayyaz , Khan Muhammad Azmat Ullah , Naz Habiba , Malik Muniba Abid Munir , Rashid Umer , Ashraf Naeem Mahmood , Afroz Amber , Shafiq Muhammad , Rao Abdul Qayyum 
Published: February 2025

ABSTRACTTomato leaf curl New Delhi virus (ToLCNDV), a begomovirus, that causes severe leaf curling, stunting, and reduced yield in tomato plants is consistently threatening its production worldwide. CRISPR/Cas9‐mediated genome editing has shown immense potential in developing disease‐resistant crops. This study successfully focuses on designing a precise and efficient strategy for in planta defence against ToLCNDV. Five key targets within the viral genome, essential for its replication and pathogenicity, were selected. Five Cas9‐expressing constructs, along with the ToLCNDV infectious clone, were agroinfiltrated into tomato plants. Three constructs effectively disrupted the ToLCNDV genome. These three constructs, 1T, 2T, and 4T, were shortlisted based on symptom severity level. They showed a relatively low viral titre of 0.5, 0.42, and 0.25 through quantitative real time PCR (qPCR) after 3, 6, and 9 days of post‐co‐infiltration, respectively. Positive control plants showed significant signs of infection like yellowing of leaves, thickening of veins, and majorly upward curling of leaves. In comparison, plants infiltrated with three Cas9 constructs had mild yellowing of leaves that recovered after approximately 21‐dpi. Furthermore, we assessed the agronomic performance of Cas9‐mediated tomato plants through in planta Agrobacterium‐mediated transformation with three short‐listed guided RNA (gRNA) constructs under greenhouse conditions. Also, qPCR analysis of Cas9 protein in 7, 14, and 21‐day of intervals gave a relative expression of 0.85, 0.76 and 0.51 respectively in genetically engineered (GE) plants through in planta transformation. In conclusion, this research contributes to CRISPR‐Cas9‐mediated plant genome editing. Our findings substantiate the efficacy of the CRISPR/Cas9 system in achieving durable engineering of resistance against ToLCNDV with 30% transformation efficiency in tomato plant. Furthermore, this study illuminates potential avenues for extending the application of this technology to confer resistance against singular and multiple infectious viruses in diverse crop species.
Sleep microstructure in patients with systemic sclerosis: A cyclic alternating pattern analysis study
Authors: Gök Duygu Kurt , Aslan‐Kara Kezban , Yıldız Burak , Türk İpek , Arslan Didem 
Published: February 2025

SummaryThe cyclic alternating pattern (CAP) is a cyclic variation of sleep electroencephalogram activity within non‐rapid eye movement (non‐REM) sleep and increased CAP is the marker of sleep instability. The present study aimed to examine the expression of cyclic alternating pattern in non‐REM sleep in patients with systemic sclerosis (SSc) and to compare these findings with the control group. Thirty‐one patients with SSc were compared with matched controls by age, gender, body mass index, and apnea–hypopnea index (AHI). Sleep measurements were obtained by standard polysomnography with conventional sleep scoring. A CAP analysis was performed and verified by certified somnologists. The sleep parameters of the SSc and control groups were similar for sleep efficiency, sleep stage percents N1, N2, N3, sleep latency, and minimum oxygen saturation (p = 0.627, p = 0.693, p = 0.530, p = 0.736, p = 0.772, and p = 0.693, respectively). The SSc patients presented a higher arousal and periodic limb movement index. The CAP analyses showed that SSc patients had higher CAP numbers, phase A2 index, A3 duration, and lower A1 duration (p = 0.032, p = 0.016, p = 0.016, and p = 0.016, respectively). Overall the phase A2 index, A2 total time during non‐REM sleep were significantly higher and the A1 duration was significantly lower in SSc patients, although sleep efficiency and the distribution of sleep macro‐structure seem to be similar to the control group. This study emphasises that microstructure analysis performed with CAP provides information that would otherwise be lost if only macrostructure analysis were considered.