bluuebunny/crossref_metadata_2025_split huggingface dataset:
Light‐Based 3D Multi‐Material Printing of Micro‐Structured Bio‐Shaped, Conducting and Dry Adhesive Electrodes for Bioelectronics
Authors: Dominguez‐Alfaro Antonio , Mitoudi‐Vagourdi Eleni , Dimov Ivan , Picchio Matias L. , Lopez‐Larrea Naroa , de Lacalle Jon Lopez , Tao Xudong , Serrano Ruben Ruiz‐Mateos , Gallastegui Antonela , Vassardanis Nikolaos , Mecerreyes David , Malliaras George G. 
Published: January 2024

AbstractIn this work, a new method of multi‐material printing in one‐go using a commercially available 3D printer is presented. The approach is simple and versatile, allowing the manufacturing of multi‐material layered or multi‐material printing in the same layer. To the best of the knowledge, it is the first time that 3D printed Poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) micro‐patterns combining different materials are reported, overcoming mechanical stability issues. Moreover, the conducting ink is engineered to obtain stable in‐time materials while retaining sub‐100 µm resolution. Micro‐structured bio‐shaped protuberances are designed and 3D printed as electrodes for electrophysiology. Moreover, these microstructures are combined with polymerizable deep eutectic solvents (polyDES) as functional additives, gaining adhesion and ionic conductivity. As a result of the novel electrodes, low skin impedance values showed suitable performance for electromyography recording on the forearm. Finally, this concluded that the use of polyDES conferred stability over time, allowing the usability of the electrode 90 days after fabrication without losing its performance. All in all, this demonstrated a very easy‐to‐make procedure that allows printing PEDOT:PSS on soft, hard, and/or flexible functional substrates, opening up a new paradigm in the manufacturing of conducting multi‐functional materials for the field of bioelectronics and wearables.
Protozoan‐Derived Cytokine‐Transgenic Macrophages Reverse Hepatic Fibrosis
Authors: Chen Ying , Wang Jie , Zhou Nan , Fang Qi , Cai Haijian , Du Zhuoran , An Ran , Liu Deng , Chen Xuepeng , Wang Xinxin , Li Fangmin , Yan Qi , Chen Lijian , Du Jian 
Published: January 2024

AbstractMacrophage therapy for liver fibrosis is on the cusp of meaningful clinical utility. Due to the heterogeneities of macrophages, it is urgent to develop safer macrophages with a more stable and defined phenotype for the treatment of liver fibrosis. Herein, a new macrophage‐based immunotherapy using macrophages stably expressing a pivotal cytokine from Toxoplasma gondii, a parasite that infects ≈ 2 billion people is developed. It is found that Toxoplasma gondii macrophage migration inhibitory factor‐transgenic macrophage (Mφtgmif) shows stable fibrinolysis and strong chemotactic capacity. Mφtgmif effectively ameliorates liver fibrosis and deactivates aHSCs by recruiting Ly6Chi macrophages via paracrine CCL2 and polarizing them into the restorative Ly6Clo macrophage through the secretion of CX3CL1. Remarkably, Mφtgmif exhibits even higher chemotactic potential, lower grade of inflammation, and better therapeutic effects than LPS/IFN‐γ‐treated macrophages, making macrophage‐based immune therapy more efficient and safer. Mechanistically, TgMIF promotes CCL2 expression by activating the ERK/HMGB1/NF‐κB pathway, and this event is associated with recruiting endogenous macrophages into the fibrosis liver. The findings do not merely identify viable immunotherapy for liver fibrosis but also suggest a therapeutic strategy based on the evolutionarily designed immunomodulator to treat human diseases by modifying the immune microenvironment.
Asymmetric Copper‐Sulphur Sites Promote C–C Coupling for Selective CO2 Electroreduction to C2 Products
Authors: Liang Liang , Yang Li , Heine Thomas , Arinchtein Aleks , Wang Xingli , Hübner Jessica , Schmidt Johannes , Thomas Arne , Strasser Peter 
Published: January 2024

AbstractSustainable multicarbon e‐chemicals are of particular interest due to their potential future, high market values, and demand. In the direct electrocatalytic formation of multicarbon e‐chemicals from CO2, the elementary C–C coupling by CO dimerization is considered the rate‐limiting step. Here, a generalized surface structural design principle of asymmetric metal pair sites is proposed, explored, and experimentally tested in order to promote CO dimerization on surfaces. First a computational model of N‐doped Cu2S layers featuring adjacent, electronically asymmetric Cuδ1+‐Cuδ2+ (0 < δ1+ < δ2+ < 1) metal atomic pairs evidenced by their non‐uniform charge distribution is considered. The electronic asymmetry resulted in distinct CO adsorption energies and the associated self‐adjusting structures, which lowered C–C coupling energy barriers significantly. The computational hypotheses are experimentally tested using X‐ray photoelectron spectroscopy of Cu‐N moieties within N‐doped Cu2S layers. In‐situ Fourier‐transform infrared spectroscopy confirms linear *CO and *CO‐CO adsorption configuration by the peaks of ≈2080 and 1920 cm−1, respectively. After N‐doping, the catalytically C2 faradaic efficiency can significantly be elevated to 14.72% due to the promotion of C–C coupling.
Ein modifiziertes Ankerpeptid LCI mit einem Cobalt‐Cofaktor verbessert die Oxidationseffizienz von Polystyrol‐Mikropartikeln
Authors: Wang Dong , Ingram Aaron A. , Luka Julian , Mao Maochao , Ahrens Leon , Bienstein Marian , Spaniol Thomas P. , Schwaneberg Ulrich , Okuda Jun 
Published: January 2024

AbstractEin typischer Bestandteil von Polymerabfällen ist Polystyrol (PS), das in zahlreichen Anwendungen eingesetzt wird, aufgrund seiner hydrophoben Eigenschaften in der Umwelt aber nur langsam abgebaut wird. Um die Reaktivität von Polystyrol zu erhöhen, müssen polare Gruppen eingeführt werden. In dieser Arbeit werden biohybride Katalysatoren auf Grundlage des modifizerten Ankerpeptids LCI_F16C vorgestellt, die sich an Polystyrol‐Mikropartikel anheften können und benzylische C−H‐Bindungen in Polystyrol‐Mikropartikeln mit handelsüblichem Oxon als Oxidationsmittel hydroxylieren. Eine dichte Oberflächenbedeckung von PS mit LCI‐Peptid erfolgt durch Bildung von Monoschichten innerhalb von Minuten in wässrigen Lösungen bei Raumtemperatur. Der katalytisch aktive Cobalt‐Cofaktor Co‐L1 oder Co‐L2 mit einem modifizierten NNNN‐makrozyklischen TACD‐Liganden (TACD=1,4,7,10‐Tetraazacyclododecan) ist über einen Maleimid‐Linker kovalent an das Ankerpeptid LCI gebunden. Im Vergleich zu den freien Cofaktoren wurde eine 12‐ bis 15‐fache Verbesserung der katalytischen Aktivität mit Biohybrid‐Katalysatoren auf Basis von LCI_F16C beobachtet.
Aromatic Ketones as Mild Presodiating Reagents toward Cathodes for High‐Performance Sodium‐Ion Batteries
Authors: Zhang Shihao , Wang Jing , Chen Kean , Pu Xiangjun , Zhu Huiying , Zhao Yanan , Zhao Along , Chen Xiaoyang , Fang Yongjin , Chen Zhongxue , Cao Yuliang 
Published: January 2024

AbstractChemical presodiation (CP) is an effective strategy to enhance energy density of sodium ion batteries. However, the sodiation reagents reported so far are basically polycyclic aromatic hydrocarbons (PAHs) wth low reductive potential (~0.1 V vs. Na+/Na), which could easily cause over‐sodiation and structural deterioration of the presodiated cathodes. In this work, Aromatic ketones (AKs) are rationally designed as mild presodiating reagents by introducing a carbonyl group (C=O) into PAHs to balance the conjugated and inductive effect. As the representatives, two compounds 9‐Fluorenoneb (9‐FN) and Benzophenone (BP) manifest favorable equilibrium potential of 1.55 V and 1.07 V (vs. Na+/Na), respectively. Note that 9‐FN demonstrates versatile presodiating capability toward multiple Na uptake hosts (tunneled Na0.44MnO2, layered Na0.67Ni0.33Mn0.67O2, polyanionic Na4Fe2.91(PO4)2P2O7, Na3V2(PO4)3 and Na3V2(PO4)2F3), enabling greatly improved initial charging capacity of the cathode to balance the irrevisible capacity of the anode. Our results indicate that the Aromatic ketones are competitive presodiating cathodic reagents for high‐performance sodium‐ion batteries, and will inspire more studies and application attempts in the future.
Asymmetric Anion Zinc Salt Derived Solid Electrolyte Interphase Enabled Long‐Lifespan Aqueous Zinc Bromine Batteries
Authors: Chen Shengmei , Li Shimei , Ma Longtao , Ying Yiran , Wu Zhuoxi , Huang Haitao , Zhi Chunyi 
Published: January 2024

AbstractOrganic additives with high‐reduction potentials are generally applied in aqueous electrolytes to stabilize the Zn anode, while compromise safety and environmental compatibility. Highly concentrated water‐in‐salt electrolytes have been proposed to realize the high reversibility of Zn plating/stripping; however, their high cost and viscosity hinder their practical applications. Therefore, exploring low‐concentration Zn salts, that can be used directly to stabilize Zn anodes, is of primary importance. Herein, we developed an asymmetric anion group, bi(difluoromethanesulfonyl)(trifluoromethanesulfonyl)imide (DFTFSI‐)‐based novel zinc salt, Zn(DFTFSI)2, to obtain a high ionic conductivity and a highly stable dendrite‐free Zn anode. Experimental tests and theoretical calculations verified that DFTFSI− in the Zn2+ solvation sheath and inner Helmholtz plane would be preferentially reduced to construct layer‐structured SEI films, inhibiting hydrogen evolution and side reactions. Consequently, the Zn
Zn symmetric cell with 1M Zn(DFTFSI)2 aqueous electrolyte delivers an ultralong cycle life for >2500 h outperforming many other conventional Zn salt electrolytes. The Zn
Br2 battery also exhibits a long lifespan over 1200 cycles at ~99.8 % Coulombic efficiency with a high capacity retention of 92.5 %. Furthermore, this outstanding performance translates well to a high‐areal‐capacity Zn
Br2 battery (~5.6 mAh ⋅ cm‐2), cycling over 320 cycles with 95.3 % initial capacity retained.
Engineered Anchor Peptide LCI with a Cobalt Cofactor Enhances Oxidation Efficiency of Polystyrene Microparticles
Authors: Wang Dong , Ingram Aaron A. , Luka Julian , Mao Maochao , Ahrens Leon , Bienstein Marian , Spaniol Thomas P. , Schwaneberg Ulrich , Okuda Jun 
Published: January 2024

AbstractA typical component of polymer waste is polystyrene (PS) used in numerous applications, but degraded only slowly in the environment due to its hydrophobic properties. To increase the reactivity of polystyrene, polar groups need to be introduced. Here, biohybrid catalysts based on the engineered anchor peptide LCI_F16C are presented, which are capable of attaching to polystyrene microparticles and hydroxylating benzylic C−H bonds in polystyrene microparticles using commercially available oxone as oxidant. LCI peptides achieve a dense surface coverage of PS through monolayer formation within minutes in aqueous solutions at ambient temperature. The catalytically active cobalt cofactor Co‐L1 or Co‐L2 with a modified NNNN macrocyclic TACD ligand (TACD=1,4,7,10‐tetraazacyclododecane) is covalently bound to the anchor peptide LCI through a maleimide linker. Compared to the free cofactors, a 12‐ to 15‐fold improvement in catalytic activity using biohybrid catalysts based on LCI_F16C was observed.
Meltable Aluminum Molecular Rings with Fluorescence and Nonlinear Optical Properties
Authors: Wang San‐Tai , Fang Wei‐Hui , Zhang Jian 
Published: January 2024

AbstractCrystal–liquid–glass, which combines the tunable properties of crystalline compounds with the processability of glasses, has emerged as a new class of materials for fabricating bulk‐shapable devices in real applications. Inspired by the characteristics of deep eutectic solvent (DES) mixtures involving significant depressions in melting points compared to their neat constituent components, in this study, we designed and synthesized the first examples of meltable aluminum oxo clusters (AlOCs) via lattice doping with DESs at the molecular level. The abundant and strong hydrogen bonding between the aluminum molecular ring, DES components, and lattice solvents is postulated to be the root that affords melting point depressions and, thus, “melting” clusters. We prepared a transparent bubble‐free glass film under autogenous pressure using a hot‐press method. These cluster‐based films exhibited luminescent and nonlinear optical properties similar to those of pristine crystalline compounds. Our study belongs to the interdisciplinary disciplines of chemistry and physics. It not only breaks the limitations of crystalline glass on metal and ligand types but also acts as a general guide for extending the range of meltable crystalline materials.
Selective Formation of Pd‐DNA Hybrids Using Tailored Palladium‐Mediated Base Pairs: Towards Heteroleptic Pd‐DNA Systems
Authors: Pérez‐Romero Antonio , Cano‐Muñoz Mario , López‐Chamorro Carmen , Conejero‐Lara Francisco , Palacios Oscar , Dobado José A. , Galindo Miguel A. 
Published: January 2024

AbstractThe formation of highly organized metal‐DNA structures has significant implications in bioinorganic chemistry, molecular biology and material science due to their unique properties and potential applications. In this study, we report on the conversion of single‐stranded polydeoxycytidine (dC15) into a Pd‐DNA supramolecular structure using the [Pd(Aqa)] complex (Aqa=8‐amino‐4‐hydroxyquinoline‐2‐carboxylic acid) through a self‐assembly process. The resulting Pd‐DNA assembly closely resembles a natural double helix, with continuous [Pd(Aqa)(C)] (C=cytosine) units serving as palladium‐mediated base pairs, forming interbase hydrogen bonds and intrastrand stacking interactions. Notably, the design of the [Pd(Aqa)] complex favours the interaction with cytosine, distinguishing it from our previously reported [Pd(Cheld)] complex (Cheld=chelidamic acid). This finding opens possibilities for creating heteroleptic Pd‐DNA hybrids where different complexes specifically bind to nucleobases. We confirmed the Pd‐DNA supramolecular structural assembly and selective binding of the complexes using NMR spectroscopy, circular dichroism, mass spectrometry, isothermal titration calorimetry, and DFT calculations.
Upcycling Food By‐products: Characteristics and Applications of Nanocellulose
Authors: Kim Mikyung , Doh Hansol 
Published: January 2024

AbstractRising global food prices and the increasing prevalence of food insecurity highlight the imprudence of food waste and the inefficiencies of the current food system. Upcycling food by‐products holds significant potential for mitigating food loss and waste within the food supply chain. Food by‐products can be utilized to extract nanocellulose, a material that has obtained substantial attention recently due to its renewability, biocompatibility, bioavailability, and a multitude of remarkable properties. Cellulose nanomaterials have been the subject of extensive research and have shown promise across a wide array of applications, including the food industry. Notably, nanocellulose possesses unique attributes such as a surface area, aspect ratio, rheological behavior, water absorption capabilities, crystallinity, surface modification, as well as low possibilities of cytotoxicity and genotoxicity. These qualities make nanocellulose suitable for diverse applications spanning the realms of food production, biomedicine, packaging, and beyond. This review aims to provide an overview of the outcomes and potential applications of cellulose nanomaterials derived from food by‐products. Nanocellulose can be produced through both top‐down and bottom‐up approaches, yielding various types of nanocellulose. Each of these variants possesses distinctive characteristics that have the potential to significantly enhance multiple sectors within the commercial market.
Design patterns of biological cells
Authors: Andrews Steven S. , Wiley H. Steven , Sauro Herbert M. 
Published: January 2024

AbstractDesign patterns are generalized solutions to frequently recurring problems. They were initially developed by architects and computer scientists to create a higher level of abstraction for their designs. Here, we extend these concepts to cell biology to lend a new perspective on the evolved designs of cells' underlying reaction networks. We present a catalog of 21 design patterns divided into three categories: creational patterns describe processes that build the cell, structural patterns describe the layouts of reaction networks, and behavioral patterns describe reaction network function. Applying this pattern language to the E. coli central metabolic reaction network, the yeast pheromone response signaling network, and other examples lends new insights into these systems.
Application of a white and green spectrofluorimetric approach for facile quantification of amlodipine, a hypotensive drug, in batch materials, dosage forms, and biological fluids; content homogeneity testing
Authors: Alqarni Abdulmalik M. , Haredy Ahmed M. , Abdelrahman Kamal S. , Soltan Osama M. , Abdel‐Aal Mohamed A. A. , Alrofaidi Mohammad A. , Aalamri Abdulwahab , Osman Mhdia Elhadi , Alamri Ahmed Awadh , Hamad Ahmed Abdulhafez 
Published: January 2024

AbstractThe suggested study adheres to a particular protocol to ensure that the process is environmentally friendly and sustainable. It is worth mentioning that several tools have been adopted as prospective measures of the method greenness. Fortunately, the established analytical method is identified as white by the white analytical chemistry (WAC) concept, which uses the red/ green/blue color scheme (RGB 12 tool) to combine ecological and functional factors for the first time in studying of the cited drug. Amlodipine (AMD), a cardiovascular treating agent, belongs to the dihydropyridine class of oral calcium channel‐blocking agents. This article presents a novel, simple, green, one‐pot‐processed, fast, and ultrasensitive fluorimetric approach for monitoring and assessment of AMD using molecular‐size‐dependent fluorescence augmentation of the light scattering‐driven signal of eosin, a biological stain at a wavelength of 415 nm. This enhancement was directly proportional to the size of the produced complex. The linearity range was from 30 to 900 ng mL−1, with corresponding sensitivity limits (detection and quantitation levels) of 9.2 and 28 ng mL−1, respectively. The planned approach was also successfully used to track AMD content in bulk, dosage forms, and bio‐fluids (human plasma and urine). The developed method's eco‐friendliness was established by different eco‐rating metric tools.
Enhancing diaphragmatic defect repair and regeneration: How biomaterials leading the way to progress?
Authors: Farahani Mojtaba , Gnatowski Przemysław , Najafloo Raziyeh , Navaei Tina , Piłat Edyta , Kucińska‐Lipka Justyna , Saeb Mohammad Reza , Mozafari Masoud 
Published: January 2024

AbstractPhysicians encounter significant challenges in dealing with large diaphragmatic defects in both pediatric and adult populations. Diaphragmatic hernias, such as Morgagni, Bochdalek, and Hiatus hernias, can result in congenital lesions that are often undiagnosed until the appearance of symptoms (bleeding, anemia, and acid reflux). Therefore, substantial potential exists for developing tissue‐engineered constructs as novel therapeutic options in clinics. Recent research indicates promising mid‐term performance for both natural and synthetic materials. However, studies exploring their application in diaphragm regeneration are limited and remain in the early research stages. Additionally, further investigation is required to address the constraints in human tissue supply for clinical implementation. This article comprehensively reviews the role of biomaterials in diaphragmatic tissue repair and regeneration. It emphasizes biomaterials, including biomimetic polymers used in technological solutions. This summary will enable researchers to critically assess the capability of existing natural biomaterials as essential tissue‐engineered patches for clinical use.
A custom 3D printed paddlewheel improves growth in flat panel photobioreactor
Authors: Meagher Michelle , Tamburro Jacob , Boyle Nanette R. 
Published: January 2024

AbstractOne of the main challenges with using flat panel photobioreactors for algal growth is uneven mixing and settling of cells in corners, especially when bubbling is the only method used for mixing. In order to improve mixing in our flat panel reactor, we designed a custom paddlewheel. Paddlewheels are frequently used in outdoor algae raceway ponds to improve mixing and we are taking advantage of the same principle for mixing in the reactor. The paddlewheel is easily integrated into our PSI FMT150 1‐L flat panel photobioreactor and is printed on a 3D printer using high temperature poly lactic acid (HT‐PLA). With the inclusion of an annealing step, the paddlewheel is autoclavable. Addition of the paddlewheel in the reactor minimized cell settling and improved algal growth, as evidenced by a nearly 40% increase in oxygen production rates. Nutrient dispersion and utilization in the culture was also improved as evidenced by a corresponding 38% decrease in CO2 concentration. The paddlewheel device presented here is a cost‐effective method for improving algal growth in a flat panel photobioreactor.
Unravelling HDAC Selectivity for Erasing Acetyl Mark on Lys‐5 of Histone H2B
Authors: Shukla Shagun , Murmu Sumit , Mora Tulasiram , Dhanasekaran Karthigeyan , Roy Rajendra P. 
Published: January 2024

AbstractThe reversible acetylation of specific Lysine residues of histones plays crucial role in the epigenetic regulation of chromatin activity. Importantly, perturbations of acetylation‐deacetylation dynamics have important implications for cancer and neurological disorders. There are 18 human HDACs including sirtuins. The site‐selective acetyl eraser specificity of HDACs is poorly defined. Deciphering the site specificity preference of HDACs from a gamut of lysine in histones may be critical for targeted inhibitor development and delineation of regulatory mechanisms associated with chromatin. Here, we have interrogated the propensity of HDACs to erase acetyl mark at Lys‐5 of H2B namely, H2BK5Ac engineered by a peptide ligation reaction catalyzed by transpeptidase sortase. HDACs and Sirtuins were individually over‐expressed in HEK293 cells and the deacetylation propensity of respective cell lysates was evaluated against H2BK5Ac for initial screening of potential acetyl erasers. This screen indicated HDAC1 as the prime eraser of acetyl mark in H2BK5Ac. The propensity of HDAC1 to erase acetyl mark of H2BK5Ac was further probed using semisynthetic designer nucleosomes with whole cell lysates, recombinant enzyme, and specific inhibitors. Consistent with the above data, siRNA knockdown of HDAC1 and closely related HDAC3 in HEK293 cells prevented the loss of H2BK5 acetylation.