bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional and molecular mapping of uncoupling between vascular permeability and loss of vascular maturation in ovarian carcinoma xenografts: The role of stroma cells in tumor angiogenesis
Authors: Gilad Assaf A. , Israely Tomer , Dafni Hagit , Meir Gila , Cohen Batya , Neeman Michal 
Published: May 2005

AbstractMaintaining homogeneous perfusion in tissues undergoing remodeling and vascular expansion requires tight orchestration of the signals leading to endothelial sprouting and subsequent recruitment of perivascular contractile cells and vascular maturation. This regulation, however, is frequently disrupted in tumors. We previously demonstrated the role of tumor‐associated myofibroblasts in vascularization and exit from dormancy of human ovarian carcinoma xenografts in nude mice. The aim of this work was to determine the contribution of stroma‐ and tumor cell‐derived angiogenic growth factors to the heterogeneity of vascular permeability and maturation in MLS human ovarian carcinoma tumors. We show by RT‐PCR and by in situ hybridization that VEGF was expressed by the tumor cells, while angiopoietin‐1 and ‐2 were expressed only by the infiltrating host stroma cells. Vascular maturation was detected in vivo by vasoreactivity to hypercapnia, measured by BOLD contrast MRI and validated by immunostaining of histologic sections to α‐smooth muscle actin. Vascular permeability was measured in vivo by dynamic contrast‐enhanced MRI using albumin‐based contrast material and validated in histologic sections by fluorescent staining of the biotinylated contrast material. MRI as well as histologic correlation maps between vascular maturation and vascular permeability revealed a wide range of vascular phenotypes, in which the distribution of vascular maturation and vasoreactivity did not overlap spatially with reduced permeability. The large heterogeneity in the degree of vascular maturation and permeability is consistent with the differential expression pattern of VEGF and angiopoietins during tumor angiogenesis. © 2005 Wiley‐Liss, Inc.
Oxidative addition of the ethane CC bond to Pd. An ab initio benchmark and DFT validation study
Authors: De Jong G. Theodoor , Geerke Daan P. , Diefenbach Axel , Solà Miquel , Bickelhaupt F. Matthias 
Published: May 2005

AbstractWe have computed a state‐of‐the‐art benchmark potential energy surface (PES) for the archetypal oxidative addition of the ethane CC bond to the palladium atom and have used this to evaluate the performance of 24 popular density functionals, covering LDA, GGA, meta‐GGA, and hybrid density functionals, for describing this reaction. The ab initio benchmark is obtained by exploring the PES using a hierarchical series of ab initio methods [HF, MP2, CCSD, CCSD(T)] in combination with a hierarchical series of five Gaussian‐type basis sets, up to g polarization. Relativistic effects are taken into account either through a relativistic effective core potential for palladium or through a full four‐component all‐electron approach. Our best estimate of kinetic and thermodynamic parameters is −10.8 (−11.3) kcal/mol for the formation of the reactant complex, 19.4 (17.1) kcal/mol for the activation energy relative to the separate reactants, and −4.5 (−6.8) kcal/mol for the reaction energy (zero‐point vibrational energy‐corrected values in parentheses). Our work highlights the importance of sufficient higher angular momentum polarization functions for correctly describing metal‐d‐electron correlation. Best overall agreement with our ab initio benchmark is obtained by functionals from all three categories, GGA, meta‐GGA, and hybrid DFT, with mean absolute errors of 1.5 to 2.5 kcal/mol and errors in activation energies ranging from −0.2 to −3.2 kcal/mol. Interestingly, the well‐known BLYP functional compares very reasonably with a slight underestimation of the overall barrier by −0.9 kcal/mol. For comparison, with B3LYP we arrive at an overestimation of the overall barrier by 5.8 kcal/mol. On the other hand, B3LYP performs excellently for the central barrier (i.e., relative to the reactant complex) which it underestimates by only −0.1 kcal/mol. © 2005 Wiley Periodicals, Inc. J Comput Chem 26: 1006–1020, 2005
DFT study and Monte Carlo simulation on proton transfers of 2‐amino‐2‐oxazoline, 2‐amino‐2‐thiazoline, and 2‐amino‐2‐imidazoline in the gas phase and in water
Authors: Xue Ying , Kim Chan Kyung , Guo Yong , Xie Dai Qian , Yan Guo Sen 
Published: May 2005

AbstractDensity functional theory (DFT) and Monte Carlo (MC) simulation with free energy perturbation (FEP) techniques have been used to study the tautomeric proton transfer reaction of 2‐amino‐2‐oxazoline, 2‐amino‐2‐thiazoline, and 2‐amino‐2‐imidazoline in the gas phase and in water. Two reaction pathways were considered: the direct and water‐assisted transfers. The optimized structures and thermodynamic properties of stationary points for the title reaction system in the gas phase were calculated at the B3LYP/6‐311+G(d, p) level of theory. The potential energy profiles along the minimum energy path in the gas phase and in water were obtained. The study of the solvent effect of water on the proton transfer of 2‐amino‐2‐oxozoline, 2‐amino‐2‐thiazoline, and 2‐amino‐2‐imidazoline indicates that water as a solvent is favorable for the water‐assisted process and slows down the rate of the direct transfer pathway. © 2005 Wiley Periodicals, Inc. J Comput Chem 26: 994–1005, 2005
Clinical psychologists across the years: The division of clinical psychology from 1960 to 2003
Authors: Norcross John C. , Karpiak Christie P. , Santoro Shannon O. 
Published: May 2005

AbstractFor more than 40 years researchers have studied the members of the American Psychological Association's (APA) Division of Clinical Psychology to obtain information about their demographic characteristics, educational experiences, theoretical orientations, employment settings, professional activities, publication histories, and career satisfactions. We summarize the results from the most recent study (N = 694, 46% return rate) in the historical context of the previous findings, dating back to 1960. We provide both contemporary and historical portraits of American clinical psychology. Among the most notable trends are a steady increase in female psychologists, a decline in psychological assessment in general and projective testing in particular, the modal eclectic/integrative orientation being rivaled by the cognitive orientation, a pattern of high career satisfaction, and continued enthusiasm for the Boulder model. © 2005 Wiley Periodicals, Inc. J Clin Psychol 61: 1467–1483, 2005.
Disruption of protein–protein interactions: Towards new targets for chemotherapy
Authors: Loregian Arianna , Palù Giorgio 
Published: May 2005

AbstractProtein–protein interactions play a key role in various mechanisms of cellular growth and differentiation, and in the replication of pathogen organisms in host cells. Thus, inhibition of these interactions is a promising novel approach for rational drug design against a wide number of cellular and microbial targets. In the past few years, attempts to inhibit protein–protein interactions using antibodies, peptides, and synthetic or natural small molecules have met with varying degrees of success, and these will be the focus of this review. © 2005 Wiley‐Liss, Inc.
Loss of E‐cadherin mediated cell–cell adhesion as an early trigger of apoptosis induced by photodynamic treatment
Authors: Galaz Sergio , Espada Jesús , Stockert Juan C. , Pacheco María , Sanz‐Rodríguez Francisco , Arranz Rocío , Rello Santiago , Cañete Magdalena , Villanueva Angeles , Esteller Manel , Juarranz Angeles 
Published: May 2005

AbstractPhotodynamic treatment with different photosensitizers (PSs) can result in the specific induction of apoptosis in many cell types. It is commonly accepted that this apoptotic response depends on the mitochondrial accumulation of the PS. Accumulation in other cellular organelles, such as lysosomes or the Golgi complex, and subsequent photodamage resulting in an apoptotic process has been also described. However, the role played by cell adhesion in apoptosis induced in epithelial cells after photodynamic treatment is not well characterized. Here, we have used a murine keratinocyte line, showing a strong dependence on E‐cadherin for cell–cell adhesion and survival, to analyze the relevance of this adhesion complex in the context of zinc(II)‐phthalocyanine (ZnPc) photodynamic treatment. We report that under apoptotic conditions, ZnPc phototreatment induces a rapid disorganization of the E‐cadherin mediated cell–cell adhesion, which largely preceded both the detachment of cells from the substrate, via β‐1 integrins and the induction of apoptotic mitochondrial markers. Therefore, the alteration in E‐cadherin, α‐ and β‐catenins adhesion proteins preceded the release of cytochrome c (cyt c) from mitochondria to the cytosol and the activation of caspase 3. In addition, blocking E‐cadherin function with a specific antibody (Decma‐1) induced apoptosis in this cell system. These results strongly suggest that the E‐cadherin adhesion complex could be the primary target of ZnPc phototreatment, and that loss of E‐cadherin mediated cell adhesion after early photodamage triggers an apoptotic response. © 2005 Wiley‐Liss, Inc.
Mdm2 and HIF‐1α interaction in tumor cells during hypoxia
Authors: Nieminen Anna‐Liisa , Qanungo Suparna , Schneider Elizabeth A. , Jiang Bing‐Hua , Agani Faton H. 
Published: May 2005

AbstractThe interaction between HIF‐1α, Mdm2, and p53 proteins during hypoxia has received recent attention. Here, we investigated the consequences of interaction between HIF‐1α and Mdm2 under hypoxic conditions. Endogenous HIF‐1α and Mdm2 proteins were co‐immunoprecipitated from lysates of hypoxic HCT116 p53WT and p53−/− cells, suggesting that association of these two proteins is a p53‐independent event. The cellular Mdm2 protein content was not significantly altered in hypoxic tumor cells. Overexpression of Mdm2 resulted in an increase in HIF‐1α protein content in hypoxic cells and increased hypoxia‐induced vascular endothelial growth factor (VEGF) transcriptional activation. These results point toward a novel and p53‐independent function of Mdm2 to promote tumor cell adaptations to hypoxia by interacting with and promoting HIF‐1 activation. © 2005 Wiley‐Liss, Inc.
Performance of H‐ZSM‐5‐supported bimetallic catalysts for the combustion of polluting volatile organic compounds in air
Authors: Abdullah Ahmad Zuhairi , Abu Bakar Mohamad Zailani , Bhatia Subhash 
Published: May 2005

AbstractThe performance of H‐ZSM‐5‐supported bimetallic catalysts with chromium as the base metal in the combustion of ethyl acetate and benzene is reported. A reactor operated from 100 to 500 °C at a gas hourly space velocity (GHSV) of 32 000 h−1 was used for study of the activity. A combination of 1.0 wt% chromium and 0.5 wt% copper yielded a catalyst (Cr1.0Cu0.5/Z) with improved conversion and carbon dioxide yield. Cr2O3 (Cr3+) and CuO (Cu2+) were the predominant metal species in the catalyst. In agreement with the Mars–van Krevelen model, improved reducibility of Cr3+ in the presence of Cu2+ led to an improvement in activity. The copper content in Cr1.0Cu0.5/Z also favored the formation of deep combustion products. Condensation and subsequent growth of coke precursors in the catalyst pores led to the formation of a softer and less aromatic coke fraction while dehydrogenation activity on acid sites formed a harder and more aromatic coke fraction. The use of Cr1.0Cu0.5/Z favored the formation of lower molecular weight intermediates, leading to reduction in formation of softer coke. Copyright © 2005 Society of Chemical Industry
Synthesis and characterization of a synthetic heme‐based oxygen carrier
Authors: Al‐Kassim Loola , Wang Z , Szabo Arthur G , Taylor Keith E , Mutus Bulent 
Published: May 2005

AbstractWe have prepared a novel oxygen carrier composed of Starburst dendrimer 3 hematoporphyrin iron(III) di‐1‐(3‐aminopropyl)imidazole di‐succinic acid (SDHAPI). This compound has a molecular weight of ∼12.7 kD. O2 and CO binding to SDHAPI was demonstrated by UV‐vis spectroscopy. Oxygenated SDHAPI had an estimated auto‐oxidation half‐life of 24 min. The oxygen stoichiometry (∼6 mol of O2 mol−1 SDHAPI) and the kobs(on+off) of 0.084 s−1 and kobs(off) of 0.009 s−1 were estimated with an oxygen electrode. Copyright © 2005 Society of Chemical Industry
Application of artificial neural networks to the analysis of two‐dimensional fluorescence spectra in recombinant E coli fermentation processes
Authors: Lee Kum‐Il , Yim Yong‐Sik , Chung Sang‐Wook , Wei Jiaqiu , Rhee Jong Il 
Published: May 2005

AbstractA two‐dimensional (2D) spectrofluorometer was used to monitor various fermentation processes with recombinant E coli for the production of 5‐aminolevulinic acid (ALA). The whole fluorescence spectral data obtained during a process were analyzed using artificial neural networks, ie self‐organizing map (SOM) and feedforward backpropagation neural network (BPNN). The SOM‐based classification of the whole spectral data has made it possible to qualitatively associate some process parameters with the normalized weights and variances, and to select some useful combinations of excitation and emission wavelengths. Based on the classified fluorescence spectra a supervised BPNN algorithm was used to predict some of the process parameters. It was also shown that the BPNN models could elucidate some sections of the process's performance, eg forecasting the process's performance. Copyright © 2005 Society of Chemical Industry
Adsorption of selected environmentally important metals by poultry manure‐based granular activated carbons
Authors: Lima Isabel M , Marshall Wayne E 
Published: May 2005

AbstractWater quality and public health impacts of mass produced poultry manure have prompted the need for viable conversion and reuse solutions. Conversion of poultry manure to value‐added granular activated carbons for environmental remediation can be such a solution. The objective of this study was to compare the effectiveness of poultry manure‐based carbons for the adsorption of selected metal ions (copper, cadmium, nickel, zinc) from solutions containing individual ions or the four metal ions present together. Adsorption properties for poultry manure‐based carbons were compared with those of two commercial carbons, PŪR RF and Minotaur, and carbons from three traditional precursors, coal, coconut and wood. Pelletized samples were pyrolyzed at 700 °C for 1 h followed by 800 °C steam activation at 1, 3 and 5 mL min−1 water flow rate, for 30 and 45 min, under nitrogen. The carbon's ability to adsorb the metals was influenced by the activation strategy, increasing for longer activation times and higher water flow rates, in the presence of a single metal solution. Saturation conditions were achieved at 20 mM and, at 5 mM, almost all metal ion in solution was adsorbed, except for nickel. Carbons showed similar affinity towards copper, cadmium and zinc, individually. However, when in competition, at 5 mM of each metal, manure‐based carbons showed a preference for Cu2+ followed by Zn2+ and Cd2+. Poultry manure‐based carbons outperformed all reference carbons and PŪR RF, and, except for Ni2+, Minotaur was also outperformed. Experiments showed that the highest removal rate in a competition situation was obtained for broiler‐cake carbon activated for 45 min at 3 mL min−1, with 93.1%, 50.9% and 85.2% for copper, cadmium and zinc ions, respectively. Published in 2005 for SCI by John Wiley & Sons, Ltd.
Oxygen transfer effects in β‐lactamase fermentation by Bacillus licheniformis in a glucose‐based defined medium
Authors: Çalık Pınar , Arifoǧlu Müge , Çalık Güzide 
Published: May 2005

AbstractThe effects of oxygen transfer on the β‐lactamase production by Bacillus licheniformis were investigated in a glucose‐based defined medium. The experiments were conducted in 3.0 dm3 batch bioreactor systems at three different air inlet (QO/VR = 0.2, 0.5 and 1.0 vvm) and agitation rates (N = 250, 500 and 750 min−1). During the fermentation, the concentrations of the cell, glucose, by‐products, ie organic and amino acids, oxygen transfer coefficients (KLa), yield coefficients, specific rates and oxygen uptake rates (OUR) were determined, in addition to β‐lactamase activities. The highest β‐lactamase activity was obtained at QO/VR = 0.5 vvm and N = 500 min−1 and at QO/VR = 0.2 vvm and N = 500 min−1 conditions, as ca A = 90 U cm−3. The highest cell concentration was obtained as CX = 0.67 kg m−3 at QO/VR = 0.5 vvm and N = 750 min−1 and at QO/VR = 0.2 vvm and N = 750 min−1 conditions. The values of KLa increased with increasing agitation and aeration rates and varied between 0.007 and 0.044 s−1, and the OUR varied between 0.4 and 1.6 mol m−3 s−1. With increasing QO/VR and/or N, the Damköhler number (ie the oxygen transfer limitation) decreased owing to the increase in mass transfer coefficients (KLa). The highest instantaneous yield of cell on substrate (YX/S) and yield of cell on oxygen (YX/O) values were respectively obtained at 0.5 vvm and 500 min−1 conditions at t = 2 h as YX/S = 0.72 kg kg−1 and YX/O = 1.49 kg kg−1. The highest instantaneous yield of substrate on oxygen (YS/O) was obtained at 0.5 vvm and 750 min−1 conditions at t = 20 h as YS/O = 8.07 kg kg−1. Copyright © 2005 Society of Chemical Industry
Catalytic hydrothermal treatment of pine wood biomass: effect of RbOH and CsOH on product distribution
Authors: Karagöz Selhan , Bhaskar Thallada , Muto Akinori , Sakata Yusaku 
Published: May 2005

AbstractLow‐temperature hydrothermal treatment of pine wood biomass was performed in the presence of RbOH and CsOH catalysts (280 °C for 15 min). The effect of the catalysts on the distribution of products and the volatility distribution of oxygenated hydrocarbons was studied in detail. Oxygenated hydrocarbons were extracted from the liquid and solid portions and analysed individually by gas chromatography/mass spectrometry. Catalytic (RbOH and CsOH) hydrothermal treatment of wood biomass produced mainly phenolic compounds and benzenediol derivatives. The use of RbOH and CsOH catalysts hindered the formation of char and favoured the formation of oil products, as observed previously for various other base catalysts. The volatility distribution of hydrocarbons (ether extract) was characterised by carbon‐normal paraffin (C‐NP) gram and it was found that the oxygenated hydrocarbons from all runs, including thermal, were distributed in the boiling point region of n‐C6 to n‐C17. Copyright © 2005 Society of Chemical Industry
Simultaneous treatment of semiconductor wastewater and distillery slops by mixing and precipitation/coagulation
Authors: Navarro Ronald R , Navarro Rosvic C , Alfafara Catalino G , Demafelis Rex B , Tatsumi Kenji 
Published: May 2005

AbstractOrganic matter (chemical oxygen demand, COD) removal with decolorization in waste distillery slops and copper removal in a semiconductor industry wastewater were achieved in a single step mixing and precipitation/coagulation treatment system. The process utilized the complementary properties of the positively charged copper ions in semiconductor wastewater and net negative charge of melanoidin (organic chromophoric pollutant) in distillery slops to mutually neutralize each other. Copper ions served as coagulant for slops and melanoidin served as precipitant for copper. The volumetric ratio of the wastewater and pH were optimized to attain maximum removal of organic matter and copper. The optimum volumetric ratio for the evaluated semiconductor wastewater to distillery slops was found to be 2–3 with an equilibrium pH of around pH 6. At optimum conditions, average removals of COD and copper were 86% and 92%, respectively, in an actual and undiluted system. Decolorization efficiency using the diluted distillery slops was 89%. The process can be considered an effective pretreatment procedure for simultaneous gross removal of copper and color/COD, particularly in highly concentrated waste streams. Copyright © 2005 Society of Chemical Industry
Granulation of a mixture of suspended anaerobic and aerobic cultures under alternating anaerobic/microaerobic/aerobic conditions: a preliminary study
Authors: Ergüder Tuba H , Demirer Göksel N 
Published: May 2005

AbstractThis paper focuses on a new granulation study area where a mixture of suspended anaerobic and aerobic cultures was used as seed. Mixed suspended anaerobic and aerobic cultures exposed to alternating anaerobic/microaerobic/aerobic conditions developed granulates up to 2.5 mm in size. The granules developed displayed both oxygen uptake (3.83–8.75 mg dissolved oxygen g−1 volatile suspended solids h−1) and methanogenic activities (7.5 cm3 CH4 d−1) comparable to those of aerobic and anaerobic control cultures, respectively. Oxygen doses of 60, 100 and 120% of the total chemical oxygen demand added (23, 38 and 45 cm3) and ethanol (among ethanol, glucose, acetic acid, and glucose + acetic acid) resulted in the largest and the most stable granules. Copyright © 2005 Society of Chemical Industry
Role of unburnt carbon in adsorption of dyes on fly ash
Authors: Wang Shaobin , Boyjoo Yashveersingh , Choueib Ali , Ng Esther , Wu Hongwei , Zhu Zhonghua 
Published: May 2005

AbstractVarious fly ash samples with different unburnt carbon contents were collected, characterised and tested for adsorption of basic dyes, Methylene Blue and Crystal Violet, in aqueous solution. It was found that unburnt carbon plays a major role in dye adsorption. The mineral matter of fly ash has little adsorption capacity and most of the adsorption capacity of fly ash can be attributed to the unburnt carbon. The fly ash with higher unburnt carbon content will have higher adsorption capacity. For the carbon‐free fly ash, adsorption capacities for Methylene Blue and Crystal Violet are only 2 × 10−6 mol g−1 and 1.0 × 10−6 mol g−1, respectively, while the adsorption capacities for Methylene Blue and Crystal Violet on carbon‐enriched fly ash are 1.2 × 10−4 mol g−1 and 1.0 × 10−4 mol g−1, respectively. A two‐site Langmuir adsorption model best describes the adsorption isotherm. Copyright © 2005 Society of Chemical Industry
Pretreatment of Chromobacterium viscosum lipase with acetone increases its activity in sodium bis‐(2‐ethylhexyl) sulfosuccinate (AOT) reverse micelles
Authors: Talukder MMR , Zaman MM , Hayashi Y , Wu JC , Kawanishi T 
Published: May 2005

AbstractThe activity of Chromobacterium viscosum lipase for hydrolysis of olive oil in sodium bis‐(2‐ethylhexyl) sulfosuccinate (AOT) reverse micelles was increased by pretreatment with acetone. In contrast to the untreated lipase, no sharp fall in the activity of the treated lipase at higher W0 (water to AOT molar ratio) values was observed. The fluorescence emission intensity of the treated lipase in reverse micelles was higher than that of the untreated lipase but the maximal emission wavelength (λmax) was the same for both lipases. A kinetic model that considers the free substrate in equilibrium with the substrate adsorbed on the micellar surface was successfully used to better understand the activity enhancement. The Michaelis constant (Km) and substrate adsorption equilibrium constant (Kad) were reduced by lipase pretreatment with acetone whereas the maximum reaction rate (vmax) remained unaltered. Copyright © 2005 Society of Chemical Industry