bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Ultra-High Speed Rotating Shoes Offshore Indonesia - A Case History
Authors: Nkwocha C. , Hermawan A. , Fauzan A. Z. , Ardhiansyah F. , Mahadhir S. 
Published: November 2023

Abstract
This paper presents two recent cases studies for high-speed rotating shoes in the East Java Sea of Indonesia where extended reach wells have been successfully drilled and completed. Rotating shoes have been used to successfully deploy 5½ inch lower completion strings in Well A, Well B, and Well C, in 2020, 2022, and 2023 respectively.
Well A had the 9⅝ inch casing shoe at 15,492 feet MD with the final depth of 16,480 feet MD. Well B is currently the longest 8½ inch production lateral drilled (6,207 feet) in Indonesia at the time of this paper with a final well depth of 15,685 feet MD. The 9⅝ inch production casing shoe was set at 9,478 feet MD. Well C was eventually completed at a final depth of 14,305 feet MD with 9⅝ inch casing shoe at 10,662 feet MD. All laterals were drilled as 8½ holes and completed as 5½ inch oil producers.
In Well A, a rotating shoe was used as a contingency due to its high ERD ratio in comparison to previous wells drilled in the field up until that time. In Well B string rotation was not a feasible option due to well profile and the 5½ inch completions string configuration, hence the need for rotating shoes. In Well C well bore stability issues across zones prone to loss circulation resulted in multiple sidetracks from the primary borehole, one of which was carried in the production section, thus predicating the use of a rotating reamer shoe for the 5½ lower completion. Well C was eventually completed with the third sidetrack, at a final depth of 14,305 feet MD with the 9⅝ inch production casing shoe set at 10,662 feet MD. The liner hanger was successfully set with an estimated savings over $700K for successfully deploying the lower completions Well C as estimated cost of an additional clean-out run.
One reason running lower completions in these wells are successful is the use of high-speed rotating shoes. When required the rotating shoe was able to ream through obstructions while guiding the completion string into and through the drilled hole to TD. Rotating shoes are still very new to the industry and adoption is slow. However, from our experience it is evident that deployment requires appreciation of the technology and the underlying principles that make it effective, thus creating an enabling environment for proper application is paramount. This requires collaboration between end-user and supplier with good understanding of well engineering principles because the service provider will be key to a successful operation.
This paper focuses on the completion operations leading to the successful deployment of the 5½ inch lower completion strings in both Wells B and C.
A Sustainable Solution for Preventing Sustained Casing Pressure: A Gas-Tight Metal to Metal Seal
Authors: Al Marhoon Z. A. , Alsharaawi A. O. , Carragher P. , Dearmon J. 
Published: November 2023

Abstract
The engineering of an extremely unique metal alloy can avail solutions for downhole sustained casing pressure which is a common well integrity concern. SCP pressure takes place when pressure communication from the reservoir reaches to surface. This happens due to cement channeling or casing-cement micro-annulus.
The performance of the proposed system, the Thermally Deformable Annulus Packer (TDAP), has been lab-tested under downhole conditions. In particular, a full-scale version of the packer has been set in a full-scale replica of the targeted Casing Casing Annulus (the 9-5/8" × 13-3/8" CCA), in the three potential fluid medias for setting; 1) in clear brine, 2) in typical fluid formula, and 3) in a typical cement slurry. The sealing capacity of each packer has been pressure tested with both a hydro-test and a gas test with the maximum pressure allowed by the casing strength.
Examples of Eutectic alloys are those that contain Bismuth. A Bismuth-based alloy can be molten downhole to a liquid with the viscosity of water and ten times its density. Upon solidification, like the behavior of ice, these alloys expand. In the proposed methodology, this expansion is utilized to provide a metal-to-metal seal in fluid leaking-channels.
During the well construction phase, the packer is run in hole as a sleeve on the 9 5/8’’ casing. It is optimally operated in areas with higher likelihood of observing SCP pressure. The created seal would eliminate the risk fluid migration. This prevents the fluid from reaching surface when a leakage occurs.
The pressure rating of the Bismuth-alloy packer, as well as its ability to seal gas, was shown to strongly depend on the medium in which the packer was set. The best performance was observed when the packer was set in clean brine. Under such condition, the packer was able to hold the maximum pressure allowed by the casing strength with both the hydro and the gas pressure tests.
The bismuth alloy packer was able to withstand pressure that exceeds the casing rated pressure. The sealing of the pressure leakage from formation was achieved successfully and the methodology was availed as a SCP preventative practice. Finding other zonal isolation methods is key to availing more strategies for mitigating SCP issues. This technology is aimed for casing designs that have 9 5/8’’ casing as a production casing and this TDAP method, working on a pro-active prevention of SCP, is new in the industry.
In-vitro anticancer activity and Molecular Docking of Andrographolide analogous.
Authors: Yerragunta Vishwanadham , Waghrey Kavita , Raj shiv , Subhashini NJP , D Appaji 
Published: November 2023

Abstract
Andrographolide is obtained from Andrographis paniculata, it is an important herbal medicine, and it is used for cancer treatment and other various diseases. Andrographolide is the major chemical constituent of the extraction of Andrographis paniculata. It is a series of new andrographolide analogous that were designed based on structural activity relationship properties then minimized ADMET prediction and docked against potential PI3K Inhibitor (PDB ID: 3ML8) using Schrödinger software. Andrographolide analogous shows a good binding affinity towards the active site of the protein. Molecular docking compounds 5a, 5b, 5f, 5i shows the best fit to the receptor and MTT assay (MCF-7) some of the andrographolide analogous show good IC50 values compared to the doxorubicin as a standard. Compounds 5b, 5i, 5f shows the microscopic observations revealed that all test molecules exhibited notable morphological changes against MCF-7 cells compared to untreated cells.
Comparison of two different experimental environments for resistance screenings for leafhopper-transmitted wheat dwarf virus in wheat
Authors: Pfrieme Anne-Kathrin , Stahl Andreas , Pillen Klaus , Will Torsten 
Published: November 2023

Abstract
Wheat dwarf virus (WDV) causes high yield losses in wheat and other cereals and is therefore an important pathogen transmitted by the leafhopper Psammotettix alienus. Symptoms of WDV infection in wheat include dwarfing and high yield losses. Climate change will increase infestations by insect-transmitted viruses due to the increasing spread of vectors. In the context of integrated pest management, the cultivation of WDV-resistant/tolerant varieties is an effective way of controlling WDV. Evaluation of tolerant/resistant genotypes is based on inoculation with virus-bearing leafhoppers and subsequent phenotyping in gauze houses under semi-field conditions. For successful screening, it is important to ensure a uniform and reproducible inoculation of plants. Abiotic conditions, particularly temperature, have a critical influence on inoculation success, and thus variations in infection rates were observed within and between previous replicates in the field. Furthermore, the leafhopper population reared in the greenhouse had to be reestablished after each infection, which delayed the screening process. We addressed these issues by developing an improved inoculation assay in which plants are inoculated in small infection hoods in the greenhouse before being planted out in gauze houses. This procedure allows optimal environmental conditions for WDV infection of test plants and allows the plants with WDV infection to develop under natural environmental conditions, such as for symptom scoring. In addition, the virus-bearing leafhoppers were re-collected from the test plants after infection, allowing economically sustainable use of the animals. The method thus enables more reliable phenotyping by increasing infection success and testing a greater number of genotypes in a shorter time.
Evaluation of neonatal and maternal outcomes in pregnant women with Covid 19 in southern Iran: An academic tertiary referral center's experience
Authors: Davoodi Marzieh , Bahrami Reza , Najib Khadijehsadat 
Published: November 2023

Abstract
Introduction
: In the pandemic infectious disease, pregnant women and their fetuses are considered as a high-risk population. considering that pregnant mothers and babies are vulnerable groups during the covid-19 pandemic, the purpose of the present study is to determine the maternal-neonatal outcome among Covid-19 pregnant mothers.
Methods
The present study is a descriptive, analytical, retrospective study, which was conducted as a case-control study in pregnant covid-19 mothers referred to hospitals covered by Shiraz University of Medical Sciences-– the main referral center in southern Iran from march2021 to march2022. The necessary data was collected by a pre-designed checklist that includes questions on demographic information, maternal and neonatal outcomes, and the study results were analyzed by SPSS ver. 25. P-value < 0.05 was considered as the significance level in this study.
Results
The present study showed significant differences in variables related to maternal and neonatal outcomes in terms of vaginal bleeding and fetal distress, premature birth, intrauterine death, ICU admission, low birth weight and NICU admission(P-value < 0.05). Although the incidence rate of neonatal transient tachypnea and pneumonia in infants of covid-19 mothers was more than twice that of the control group, there was no significant relationship between the two groups in this respect((P-value > 0.0)). Also, although abnormal findings such as abnormal lung X-ray, thrombocytopenia and positive CRP were slightly more common in infants of mothers infected with Covid-19, there was no significant relationship between the two groups(P-value > 0.05).
Conclusion
Current data increase the risk of short-term and long-term maternal and fetal outcomes of covid-19 during pregnancy.
WITHDRAWN: Estimating The Carbon Footprint Of Dairy Cattle In Karapınar District
Authors: ERZURUM Onur 
Published: November 2023

Abstract
The increasing human population brings with it an increasing need for animal food. Animal products offered for consumption must also increase in keeping with this situation. This increase is tried to be met both by increasing productivity and by increasing the number of animals. For this purpose, intensive farming systems are becoming widespread. Carbon dioxide (CO2) and methane (CH4) gases, which trigger global warming that comes to the fore with climate changes, are released as a result of the vital activities of dairy cattle. The term carbon footprint is used to track the outputs of production and consumption stages. In this study, it was aimed to estimate the carbon footprint of milking dairy cattle in Karapınar district of Konya province in 2018–2019. For this purpose, tables and equations in the Intergovernmental Panel on Climate Change (2006) guide were used. Tier 1 method was chosen as the calculation method. The number of milked animals in 2019 is 825 heads more than in 2018. The total emission factor value from enteric fermentation in 2019 increased by 81675 kg CH4/yr compared to 2018.
Assessing the Utility of Cortisol Levels in Predicting Iatrogenic Adrenal Insufficiency in Neonates Exposed to Prolonged Steroid Courses
Authors: Rosano Kristen , Bery Saya , Marasch Jaime , Farrell Ryan , Mastrandrea Lucy D. , Ryan Rita 
Published: November 2023

Abstract

Objective
To determine whether random cortisol levels obtained in neonates to assess for secondary adrenal insufficiency (AI) after prolonged steroid exposure are predictive of central AI.
Study Design:
Data were collected on neonates born 2017–2022 who received ≥ 10 consecutive days of systemic steroids and had a cortisol level measured thereafter. Data were then collected on whether those neonates developed signs of AI or had a failed adrenocorticotropic hormone (ACTH) stimulation test.
Results
Of the 71 cortisol levels (in 67 neonates) that were analyzed, there was no difference in cortisol levels between neonates who developed AI (median cortisol level of 6.5 mcg/dl) and those who did not (median of 9.2 mcg/dl), or between those who failed their ACTH stimulation test or passed it, using Wilcoxon ranked sum tests.
Conclusion
These findings demonstrate that cortisol levels may not be helpful in identifying AI in neonates exposed to prolonged steroids.
Polyethylene Glycol-Based Polymer-Drug Conjugates: Novel Design and Synthesis Strategies for Enhanced Therapeutic Efficacy and Targeted Drug Delivery
Authors: Verma Vinay Sagar , Pandey Aakansha , Jha Arvind Kumar , Badwaik Hemant Kumar Ramchandra , Alexander Amit , . Ajazuddin 
Published: November 2023

Abstract
Due to their potential to enhance therapeutic results and enable targeted drug administration, polymer-drug conjugates that use polyethylene glycol (PEG) as both the polymer and the linker for drug conjugation have attracted much research. This study seeks to investigate recent developments in the design and synthesis of PEG-based polymer-drug conjugates, emphasising fresh ideas that fill in existing knowledge gaps and satisfy the increasing need for more potent drug delivery methods. Through an extensive review of the existing literature, this study identifies key challenges and proposes innovative strategies for future investigations. The paper presents a comprehensive framework for designing and synthesising PEG-based polymer-drug conjugates, including rational molecular design, linker selection, conjugation methods, and characterisation techniques. To further emphasise the importance and adaptability of PEG-based polymer-drug conjugates, prospective applications are highlighted, including cancer treatment, infectious disorders, and chronic ailments.
CD248-targeted BBIR-T cell therapy against late-activated fibroblasts in cardiac repair after myocardial infarction
Authors: Xie Jun , Chen Haiting , Hu Ke , Tang Qi , Wang Junzhuo , Gu Qianyu , Chen Jiayu , Hu Jiaxin , Peng Ningxin , Guo Meng , Jiang Yaohui , Xu Qingbo 
Published: November 2023

Abstract
Excessive cardiac fibrosis is a key cause of heart failure and adverse ventricular remodelling after myocardial infarction. The abnormally activated fibroblasts after scar maturation are the chief culprit. Single-cell RNA sequencing of mouse cardiac interstitial cells after myocardial infarction depicts a late-activated fibroblast subpopulation F-Act and initially identifies its characteristic antigen CD248, which is also verified in human hearts. On this basis, we develop a CD248-targeted BBIR-T cell therapy against F-Act to correct cardiac repair disorders. By embedding avidin coupled to the T cell signalling domain onto the membrane, T cells are empowered to recognize and kill target cells labelled by biotinylated antigen-specific molecules. The precise removal of F-Act after the scar matured effectively inhibits fibrotic expansion in the peri-infarct zone and improves cardiac function. This therapy provides a new idea for the treatment of cardiac fibrosis and also promotes the application of engineered T cells to non-tumour diseases.
Financing Fertility through Bank Competition? Evidence from a Quasi-Natural Experiment in China
Authors: Li Dongxu , Chen Yihui , Li Ting 
Published: November 2023

Abstract
Using the bank deregulation in China as an exogenous shock to bank competition, we investigate how it affects local fertility. We show that the deregulation significantly increases city-level birth rates. This effect is more pronounced among cities with more employment, more household savings, and higher housing affordability. We argue that the deregulation improves local capital supply and boosts local economic vibrancy, which increases households’ income and willingness of fertility. Consistent with this notion, we find that the effect of bank deregulation on birth rates is greater for cities with more entrepreneurship, more private businesses, and more innovative activities. This study sheds light upon the role of financial development in affecting local fertility.
JEL Classification: G21; J13; R11
Progressive Changes in the Protein Expression Profile of Alveolar Septa in Early-Stage Lung Adenocarcinoma
Authors: Kimura Toru , Akazawa Takashi , Mizote Yu , Nakamura Harumi , Sakaue Miki , Maniwa Tomohiro , Shintani Yasushi , Honma Keiichiro , Tahara Hideaki , Okami Jiro 
Published: November 2023

Abstract
Background
Adenocarcinomas show a stepwise progression from atypical adenomatous hyperplasia (AAH) through adenocarcinoma in situ (AIS) to invasive adenocarcinoma (IA). Immunoglobulin superfamily containing leucine-rich repeat (ISLR) is a marker of tumor-restraining cancer-associated fibroblasts (CAFs), which are distinct from conventional, strongly α-smooth muscle actin (αSMA)-positive CAFs. Fibroblast-activation protein (FAP) has been focused on as a potential therapeutic and diagnostic target of CAFs.
Methods
We investigated the changes in protein expression during adenocarcinoma progression in pre-existing alveolar septa by assessing ISLR, αSMA, and FAP expression in normal, AAH, AIS, and IA lung nodules. Fourteen AAH, seventeen AIS, and twenty IA lesions were identified and randomly sampled. Immunohistochemical analysis was performed to evaluate cancer-associated changes and FAP expression in pre-existing alveolar structures.
Results
Normal alveolar septa expressed ISLR. The ISLR level in the alveolar septa decreased in AAH and AIS tissues compared with that in normal lung tissue. The αSMA-positive area gradually increased from the adjacent lung tissue (13.3% ± 15%) to AIS (87.7% ± 14%), through AAH (70.2% ± 21%). Moreover, the FAP-positive area gradually increased from AAH (1.69% ± 1.4%) to IA (11.8% ± 7.1%), through AIS (6.11% ± 5.3%). Protein expression changes are a feature of CAFs in pre-existing alveolar septa that begin in AAH. These changes gradually progressed from AAH to IA through AIS.
Conclusions
FAP-positive fibroblasts may contribute to tumor stroma formation in early-stage lung adenocarcinoma, and this could influence the development of therapeutic strategies targeting FAP-positive CAFs for disrupting extracellular matrix formation.
Comprehensive application of AI algorithms with TCR NGS data for Glioma diagnosis
Authors: Da Lin , Zhou Kaiyue , Xiao Zhengliang , Liu Qi , Wang Xu , Huo Jiaxin , Wu Xiaoqi , Xu Pengfei , Deng Yunyun , Xiao Wenwen , Sun Tao 
Published: November 2023

Abstract
TCR detection can examine the extent of T-cell immune responses. Therefore, the article analyzed characteristic data of glioma obtained by high-throughput sequencing, to predict the disease with fewer biomarkers and higher accuracy. We downloaded data online and obtained six TCR-related diversity indices to establish a multidimensional classification system. By comparing actual presence of the 602 correlated sequences, we obtained two-dimensional and multidimensional datasets. Multiple classification methods were utilized for both datasets with the classification accuracy of multidimensional data slightly less to two-dimensional datasets. This study reduced the TCR β sequences through dimensionality reduction methods like RFECV. Consequently, using only the presence of these three sequences, the classification AUC value of 96.67% can be achieved. The combination of the three correlated TCR clones obtained at a source data threshold of 0.1 was: CASSLGGNTEAFF_TRBV12_TRBJ1-1, CASSYSDTGELFF_TRBV6_TRBJ2-2, and CASSLTGNTEAFF_TRBV12_TRBJ1-1. At 0.001, the combination was: CASSLGETQYF_TRBV12_TRBJ2-5, CASSLGGNQPQHF_TRBV12_TRBJ1-5, and CASSLSGNTIYF_TRBV12_TRBJ1-3. This method can serve as a potential diagnostic and therapeutic tool, facilitating diagnosis and treatment of glioma and other cancers.
The Effects of Sirna-mediated Knockdown of Ap-1 on Pulmonary Arterial and Right Ventricular Dysfunction Associated With Cardiac and Pulmonary Fibrosis in Cardiopulmonary Disease
Authors: Comarița Ioana Karla , Tanko Gabriela , Anghelache Iliuță Laurențiu , Georgescu Adriana 
Published: November 2023

Abstract
Background
Pulmonary hypertension (PH) is a complex multifactorial vascular pathology characterized by an increased pulmonary arterial pressure, vasoconstriction, remodelling of the pulmonary vasculature, thrombosis in situ and inflammation associated with right-side heart failure. Herein, we explored the potential beneficial effects of treatment with siRNA AP-1 on pulmonary arterial hypertension (PAH), right ventricular dysfunction along with perivascular and interstitial fibrosis in pulmonary artery-PA, right ventricle-RV and lung in an experimental animal model of monocrotaline (MCT)-induced PAH.
Methods
Golden Syrian hamsters were divided into: (1) C group-healthy animals taken as control; (2) MCT group obtained by a single subcutaneous injection of 60 mg/kg MCT at the beginning of the experiment; (3) MCT-siRNA AP-1 group received a one-time subcutaneous dose of MCT and subcutaneous injections containing 100nM siRNA AP-1, every two weeks. All animal groups received water and standard chow ad libitum for 12 weeks.
Results
In comparison with the MCT group, siRNA AP-1 treatment had significant beneficial effects on investigated tissues contributing to: (1) a reduction in TGF-β1/ET-1/IL-1β/TNF-α plasma concentrations; (2) a reduced level of cytosolic ROS production in PA, RV and lung and notable improvements regarding the ultrastructure of these tissues; a decrease of inflammatory and fibrotic marker expressions in PA (COL1A/Fibronectin/Vimentin/α-SMA/CTGF/Calponin/MMP-9), RV and lung (COL1A/CTGF/Fibronectin/α-SMA/F-actin/OB-cadherin) and an increase of endothelial marker expressions (CD31/VE-cadherin) in PA; (4) structural and functional recoveries of the PA (reduced Vel, restored vascular reactivity (NA contraction, ACh relaxation)) and RV (enlarged internal cavity diameter in diastole, increased TAPSE and PRVOFs) associated with a decrease in systolic and diastolic blood pressure, and heart rate; (5) a reduced protein expression profile of AP-1S3/ pFAK/FAK/pERK/ERK and a significant decrease in the expression levels of miRNA-145, miRNA-210, miRNA-21, and miRNA-214 along with an increase of miRNA-124 and miRNA-204.
Conclusions
The siRNA AP-1-based therapy led to an improvement of pulmonary arterial and right ventricular function accompanied by a regression of perivascular and interstitial fibrosis in PA, RV and lung and a down-regulation of key inflammatory and fibrotic markers in MCT-treated hamsters.