bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive characterization of PKHD1 mutation in human colon cancer
Authors: Han Lu , Gong Fangming , Wu Xuxiaochen , Tang Wanxiangfu , Bao Hua , Wang Yue , Wang Daizhenru , Sun Yulan , Li Peng 
Published: January 2024

AbstractIntroductionThe PKHD1 (Polycystic Kidney and Hepatic Disease 1) gene is essential for producing fibrocystin or polyductin, which is crucial in various cellular functions. Mutations in PKHD1 have been found to be involved in the development and progression of colorectal cancer (CRC). Along with APC, TP53, and KRAS, PKHD1 is one of the most frequently mutated genes in CRC. PKHD1 expression is governed by the Wnt/PCP pathway, often dysregulated in CRC. Targeting this pathway, crucial for CRC progression, could unveil potential therapeutic strategies for colon cancer treatment.MethodsThis study examined an in‐house dataset of 3702 colon cancer samples, analyzing mutation landscapes, clinical features, tumor mutational burden (TMB), microsatellite instability (MSI), and chromosomal instability (CIN) score. For the survival analysis of PKHD1 patients, survival data of 436 colon adenocarcinoma samples were obtained from TCGA dataset. Additionally, 433 samples from TCGA with RNA‐seq data were used for the assessment of immune cell infiltration and gene set enrichment analysis.ResultsPolycystic Kidney and Hepatic Disease 1 mutation was detected in 424 colon cancer patients from our in‐house cohort and was associated with increased TMB, higher MSI, and lower CIN score. Importantly, within the TCGA dataset, PKHD1 mutations were identified as an independent prognostic factor, not merely correlated with established prognostic biomarkers, and were associated with poorer overall survival outcomes. In terms of immune response, these mutations correlated with increased enrichment scores for 12 immune cell types, including B cell plasma, macrophages, and naive CD4+ T cells. Additionally, interferon alpha and interferon‐gamma gene sets were significantly down‐regulated in patients with PKHD1 mutations (FDA q‐value < 0.1).ConclusionsOverall, these findings suggest that PKHD1 may be a potential biomarker for the prognosis of colon cancer and provide some insight for personalized immunotherapy.
Bougainvillea glabra Choisy bracts extract in free and liposomal forms reduce hyperplasia induced by let‐60gain‐of‐function in Caenorhabditis elegans
Authors: de Oliveira Pereira Flávia Suelen , Oliveira de Souza Maria Eduarda , Viçozzi Gabriel Pedroso , Caurio Aline Castro , Pinton Simone , Denardin Cristiane Casagrande , Haas Sandra Elisa , Gasparotto Denardin Elton Luis , Ávila Daiana Silva 
Published: January 2024

AbstractIn this study, we evaluated the toxicological and antiproliferative effects of B. glabra Choisy bract extract (BGCE) in its free and loaded into liposomes forms administered to C. elegans mutants with let‐60 gain‐of‐function (gf). Our results demonstrated that the concentration up to 75 μg CAE/mL of BGCE was safe for the worms. Notably, we developed BGCE‐loaded liposomes to extend the pharmacological window up to 100 μg CAE/mL without toxicity. In addition, the extract and liposomes reduced the number and area of the multivulva formed in let‐60 gf mutants. There was also an increase in the apoptotic signaling in the germline cells and increased longevity mediated through DAF‐16 nuclear translocation with GST‐4 activation in the treated animals. Our findings demonstrated that the BGCE‐loaded liposomes possess antitumoral effects due to the activation of the apoptotic signaling and DAF‐16 nuclear translocation.
Perfluoralkyl/Rose Bengal Functionalized Janus Silica Nanoparticles for Photocatalytic Transformations with Singlet Oxygen
Authors: Pourshiani Omid , Romero Zeno , Ganji Nasim , Herrendorf Tim , Seifert Andreas , Demchenko Anna , Prosenc Marc H. , Kleist Wolfgang , Kopnarski Michael , Karimi Babak , Thiel Werner R. 
Published: January 2024

AbstractA Janus mesoporous silica material with an imidazolium‐based ionic liquid and perflouroalkyl motifs was synthesized using hollow Stöber particles. Its morphology, chemical composition and surface properties were characterized by N2 physisorption analysis, scanning and transmission electron microscopy, elemental mapping, thermogravimetric and elemental analysis, and solid state NMR spectroscopy. The Janus nanoparticles have independent faces with fluorophilic and hydrophilic properties suitable for interaction with oxygen and Rose Bengal, which is a known photosensitizer for singlet oxygen generation. The catalytic activity of these Janus particles was evaluated in a series of transformations using singlet oxygen. They provide the desired products in high yield and selectivity.
Zirconia‐containing glass‐ceramics: From nucleating agent to primary crystalline phase
Authors: Shearer Adam , Montazerian Maziar , Deng Binghui , Sly Jessica J. , Mauro John C. 
Published: January 2024

AbstractThis article reviews promising studies on the design, manufacturing, microstructure, properties, and applications of glass‐ceramics containing ZrO2 and relevant glass‐ceramic matrix composites. After the addition of ZrO2 to a glass‐ceramic composition, it can persist in the residual glassy phase, facilitate nucleation, and/or precipitate as ZrO2 or another zirconate crystalline phase. Also, ZrO2‐reinforced or ZrO2‐toughened glass‐ceramics can be designed as composites. In this article, the term “ZrO2‐containing glass‐ceramics” encompasses all these scenarios in which ZrO2 is present. Such glass‐ceramics offer a wide range of applications in modern industries, including but not limited to architecture, optics, dentistry, medicine, and energy. Since S. Donald Stookey's discovery of glass‐ceramics in the early 1950s, the most important scientific efforts reported in the literature are reviewed. ZrO2 is commonly added to glass‐ceramics to promote nucleation. As a result, the role of ZrO2 in structural modification of residual glass and stimulating the nucleation in glass‐ceramic is first discussed. ZrO2 can also be designed into the main crystalline phase of glass‐ceramics, contributing achieving super high fracture toughness above 4 MPa·m0.5. Experimental and computational studies are reviewed in detail to elucidate how the transformation toughening and other mechanisms help to achieve such high values of fracture toughness. Sintered and glass‐ceramic matrix composites also show promise, where ZrO2 contributes to improved stability and mechanical properties. Finally, we hope this article will provoke interest in glass‐ceramic materials in both the scientific and industrial communities so that their tremendous technological potential in developing, for example, tough, thermally stable, transparent, and biologically compatible materials can be realized more widely.
Nickel Carbide Nanoparticle Catalyst for Selective Hydrogenation of Nitriles to Primary Amines
Authors: Yamaguchi Sho , Kiyohira Daiki , Tada Kohei , Kawakami Taiki , Miura Akira , Mitsudome Takato , Mizugaki Tomoo 
Published: January 2024

AbstractDespite its unique physicochemical properties, the catalytic application of nickel carbide (Ni3C) in organic synthesis is rare. In this study, we report well‐defined nanocrystalline Ni3C (nano‐Ni3C) as a highly active catalyst for the selective hydrogenation of nitriles to primary amines. The activity of the aluminum‐oxide‐supported nano‐Ni3C (nano‐Ni3C/Al2O3) catalyst surpasses that of Ni nanoparticles. Various aromatic and aliphatic nitriles and dinitriles were successfully converted to the corresponding primary amines under mild conditions (1 bar H2 pressure). Furthermore, the nano‐Ni3C/Al2O3 catalyst was reusable and applicable to gram‐scale experiments. Density functional theory calculations suggest the formation of polar hydrogen species on the nano‐Ni3C surface, which were attributed to the high activity of nano‐Ni3C towards nitrile hydrogenation. This study demonstrates the utility of metal carbides as a new class of catalysts for liquid‐phase organic reactions.
A Torquoselective Thermal 6π‐Electrocyclization Approach to 1,4‐Cyclohexadienes via Solvent‐Aided Proton Transfer: Experimental and Theoretical Studies
Authors: Saha Jayanta , Banerjee Soumadip , Malo Sidhartha , Das Abhijit Kumar , Das Indrajit 
Published: January 2024

AbstractThe thermal 6π‐electrocyclization of hexatriene typically delivers 1,3‐cyclohexadiene (1,3‐CHD). However, there is only limited success in directly synthesizing 1,4‐cyclohexadiene (1,4‐CHD) using such an approach, probably due to the difficulty in realizing thermally‐forbidden 1,3‐hydride shift after electrocyclic ring closure. The present study shows that by heating (2E,4E,6E)‐hexatrienes bearing ester or ketone substituents at the C1‐position in a mixture of toluene/MeOH or EtOH (2 : 1) solvents at 90–100 °C, 1,4‐CHDs can be selectively synthesized. This is achieved through a torquoselective disrotatory 6π‐electrocyclic ring closure followed by a proton‐transfer process. The success of this method depends on the polar protic solvent‐assisted intramolecular proton transfer from 1,3‐CHD to 1,4‐CHD, which has been confirmed by deuterium‐labeling experiments. There are no reports to date for such a solvent‐assisted isomerization. Density functional theory (DFT) studies have suggested that forming 1,3‐CHD and subsequent isomerization is a thermodynamically feasible process, regardless of the functional groups involved. Two possible successive polar solvent‐assisted proton‐transfer pathways have been identified for isomerization.
Multimodaler Wasserstoff‐Hub in der Innovationsregion Fessenheim
Authors: Jordan Thomas 
Published: January 2024

AbstractIm Rahmen einer binationalen Machbarkeitsstudie wurden Konzepte zur Einführung nachhaltiger Technologien in der Innovationsregion Fessenheim und darauf aufbauend verschiedene Handlungsansätze für deutsch‐französische Demonstrationsprojekte entwickelt. Das übergeordnete Ziel ist die Entwicklung einer nachhaltigen und zukunftsorientierten CO2‐neutralen Wirtschaftsregion. Der Umbau von Gesellschaft und Wirtschaft hin zu einer erneuerbaren Energieversorgung und einer nachhaltigen Ressourcennutzung, verbunden mit der Schaffung von zukunftsorientierten Arbeitsplätzen, wird durch den Einsatz von Wasserstoff als Energieträger stark gefördert. Nachhaltig produzierter „grüner“ Wasserstoff ermöglicht die nahezu sofortige Substitution von fossilem Wasserstoff in industriellen Prozessen, die saisonale Speicherung von erneuerbarem Strom und die flexible und effiziente Kopplung verschiedener Energiesektoren. Die vorgeschlagenen Pilotprojekte decken die gesamte Wertschöpfungskette mit grünem Wasserstoff ab und bauen dabei auf den besonderen regionalen Eigenschaften und Gegebenheiten auf.
Greener, Safer and Better Performing Aqueous Binder for Positive Electrode Manufacturing of Sodium Ion Batteries
Authors: Xu Ruochen , Pamidi Venkat , Tang Yushu , Fuchs Stefan , Stein Helge S. , Dasari Bosubabu , Zhao‐Karger Zhirong , Behara Santosh , Hu Yang , Trivedi Shivam , Anji Reddy M. , Barpanda Prabeer , Fichtner Maximilian 
Published: January 2024

AbstractP2‐type cobalt‐free MnNi‐based layered oxides are promising cathode materials for sodium‐ion batteries (SIBs) due to their high reversible capacity and well chemical stability. However, the phase transformations during repeated (dis)charge steps lead to rapid capacity decay and deteriorated Na+ diffusion kinetics. Moreover, the electrode manufacturing based on polyvinylidene difluoride (PVDF) binder system has been reported with severely defluorination issue as well as the energy intensive and expensive process due to the use of toxic and volatile N‐methyl‐2‐pyrrolidone (NMP) solvent. It calls for designing a sustainable, better performing, and cost‐effective binder for positive electrode manufacturing. In this work, we investigated inorganic sodium metasilicate (SMS) as a viable binder in conjunction with P2‐Na0.67Mn0.55Ni0.25Fe0.1Ti0.1O2 (NMNFT) cathode material for SIBs. The NMNFT‐SMS electrode delivered a superior electrochemical performance compared to carboxy methylcellulose (CMC) and PVDF based electrodes with a reversible capacity of ~161 mAh/g and retaining ~83 % after 200 cycles. Lower cell impedance and faster Na+ diffusion was also observed in this binder system. Meanwhile, with the assistance of TEM technique, SMS is suggested to form a uniform and stable nanoscale layer over the cathode particle surface, protecting the particle from exfoliation/cracking due to electrolyte attack. It effectively maintained the electrode connectivity and suppressed early phase transitions during cycling as confirmed by operando XRD study. With these findings, SMS binder can be proposed as a powerful multifunctional binder to enable positive electrode manufacturing of SIBs and to overall reduce battery manufacturing costs.
OMIP‐100: A flow cytometry panel to investigate human neutrophil subsets
Authors: Schofield Craig J. , Tirouvanziam Rabindra , Garratt Luke W. 
Published: January 2024

AbstractThis 14‐color, 13‐antibody optimized multicolor immunofluorescence panel (OMIP) was designed for deep profiling of neutrophil subsets in various types of human samples to contextualize neutrophil plasticity in a range of healthy and diseased states. Markers present in the OMIP allow the profiling of neutrophil subsets associated with ontogeny, migration, phagocytosis capacity, granule release, and immune modulation. For panel design, we ensured that the commonly available fluorophores FITC/AF488, PE, and APC were assigned to the intracellular subset marker Olfactomedin 4, the maturity and activation marker CD10, and whole blood subset marker CD177, respectively. These markers can be easily replaced without affecting the core identification of neutrophils, enabling antibodies to new neutrophil antigens of interest or for fluorescent substrates to assess different neutrophil functions to be easily explored. Panel optimization was performed on whole blood and purified neutrophils. We demonstrate applications on clinical samples (whole blood and saliva) and experimental endpoints (purified neutrophils stimulated through an in vitro transmigration assay). We hope that providing a uniform platform to analyze neutrophil plasticity in various sample types will facilitate the future understanding of neutrophil subsets in health and disease.
Short‐term outcomes of the study of refeeding to optimize inpatient gains for patients with atypical anorexia nervosa
Authors: Garber Andrea K. , Cheng Jing , Accurso Erin C. , Buckelew Sara M. , Downey Amanda E. , Le Grange Daniel , Gorrell Sasha , Kapphahn Cynthia J. , Kreiter Anna , Moscicki Anna‐Barbara , Golden Neville H. 
Published: January 2024

AbstractObjectiveThe StRONG trial demonstrated the safety and efficacy of higher calorie refeeding (HCR) in hospitalized adolescents and young adults with malnutrition secondary to restrictive eating disorders. Here we compare refeeding outcomes in patients with atypical anorexia nervosa (atypical AN) versus anorexia nervosa (AN) and examine the impact of caloric dose.MethodPatients were enrolled upon admission and randomized to meal‐based HCR, beginning 2000 kcal/day and advancing 200 kcal/day, or lower calorie refeeding (LCR), beginning 1400 kcal/day and advancing 200 kcal every other day. Atypical AN was defined as %median BMI (mBMI) > 85. Independent t‐tests compared groups; multivariable linear and logistic regressions examined caloric dose (kcal/kg body weight).ResultsAmong n = 111, mean ± SD age was 16.5 ± 2.5 yrs; 43% had atypical AN. Compared to AN, atypical AN had slower heart rate restoration (8.7 ± 4.0 days vs. 6.5 ± 3.9 days, p = .008, Cohen's d = −.56), less weight gain (3.1 ± 5.9%mBMI vs. 5.4 ± 2.9%mBMI, p < .001, Cohen's d = .51) and greater hypomagnesemia (29% vs. 11%, p = .03, OR = 3.29). These suboptimal outcomes were predicted by insufficient caloric dose (32.4 ± 6.9 kcal/kg in atypical AN vs. 43.4 ± 9.8 kcal/kg in AN, p < .001, Cohen's d = 1.27). For every 10 kcal/kg increase, heart rate was restored 1.7 days (1.0, 2.5) faster (p < .001), weight gain was 1.6%mBMI (.8, 2.4) greater (p < .001), and hypomagnesemia odds were 70% (12, 128) lower (p = .02).DiscussionAlthough HCR is more efficacious than LCR for refeeding in AN, it contributes to underfeeding in atypical AN by providing an insufficient caloric dose relative to the greater body weight in this diagnostic group.Public significanceThe StRONG trial previously demonstrated the efficacy and safety of higher calorie refeeding in patients with malnutrition due to restrictive eating disorders. Here we show that higher calorie refeeding contributes to underfeeding in patients with atypical anorexia nervosa, including poor weight gain and longer time to restore medical stability. These findings indicate these patients need more calories to support nutritional rehabilitation in hospital.
Interannual controls on riparian plant health in a dryland river
Authors: Downs Peter W. , Lambert Adam M. , Williams Jared , Braman Charles A. 
Published: January 2024

AbstractRiparian zones in drylands provide important refugia for plants but depend on groundwater and thus are subject to local temporal and spatial variability in abiotic controls. In lieu of costly field‐based sampling, we used readily available data to establish site–scale interannual relationships among riparian plant health and the abiotic factors that control their water balance for a historically persistent wetland adjoining the Santa Clara River in southern California, USA. Non‐linear generalized additive model (GAM) analysis of plant health, represented using the normalized difference vegetation index (NDVI), confirmed robust relationships among plant health and various geomorphological and hydrological factors over multi‐decadal timeframes, including years since last high‐flow event, intra‐year groundwater elevation changes and magnitude of 2‐year cumulative surface water inflows. Geomorphic controls are related to years with high flows that cause extensive scour and deposition that re‐set riparian plant communities. Relationships with dry‐season groundwater declines reflect direct plant access to sub‐surface moisture. Hydrological dependence via cumulative inflow magnitude indicates the dependency of groundwater elevations on sufficient winter recharge to prevent precipitous groundwater decline. GAMs‐based inflection point analysis of surface water inflows versus groundwater elevations confirmed that the cumulative magnitude of multi‐year inflows is critical in avoiding catastrophic groundwater declines and that large flood events drive groundwater recovery. We show that abiotic controls on plant health can be derived from readily available data and that non‐linear analysis better represents the complexity of these scalar controls. Our analysis has relevance for ecosystem management of human‐altered rivers and climate change adaptation.
Rare Earth 2‐Methyl‐3‐furoate Complexes: Effect of Steric Hindrance on Corrosion Inhibitor Properties
Authors: Vithana Vidushi P. , Guo Zhifang , Deacon Glen B. , Junk Peter C. 
Published: January 2024

AbstractThe preparation and characterization of six rare earth 2‐methyl‐3‐furoate (2m3fur) complexes are detailed in this study. Analysis through single‐crystal X‐ray diffraction, as well as powder XRD, reveals that all six compounds belong to one of two structural groups: {[RE2(2m3fur)6(EtOH)] ⋅ H2O}n (RE=La, Ce, Pr) and [RE3(2m3fur)9]n (RE=Y, Er, Yb). These structural groups feature carboxylate coordinated linear polymers. The former complexes have two distinct metal centers, one ten coordinate and one nine, with lattice water participating in a hydrogen bond with coordinated ethanol. The latter structures, involving erbium, ytterbium, and yttrium, have three unique metal centers: two eight coordinate, and one seven‐coordinated. In corrosion inhibition tests on mild steel in 0.01 M NaCl solution, the maximum efficiency was observed with [Y3(2m3fur)9]n at 59 %, which is less effective than yttrium 3‐furoate (90 % efficiency). The results suggest that the steric effect of the methyl group adversely affects corrosion inhibition.
Synthesis of Sulfonimidamide‐Based 1,2‐Benzothiazines by [4+2] Oxidative Annulation of Sulfonimidamides and Alkynes in Water under Visible Light
Authors: Natarajan K. , Ravindra S. , Padma Priya V. R. , Kataria Ramesh , Chandra Nandi Ganesh 
Published: January 2024

AbstractHerein, we disclose a visible‐light‐induced rhodium‐catalyzed oxidative C−H/N−H bond activation of sulfonimidamides (SIAs) and subsequent [4+2] annulation with an alkyne in water to yield SIA‐based 1,2‐benzothiazines in good to high yields in short reaction times. Further, the C=C bond of the benzothiazine can be cleaved under environmentally friendly conditions (oxygen atmosphere and blue LEDs) to deliver o‐acyl‐N‐acyl SIAs. Interestingly, o‐acyl‐N‐acyl SIAs can also be synthesized directly from SIAs in very good yields without isolating the benzothiazine. The selective and efficient reduction of the benzothiazine C=C bond in the presence of InCl3/R3SiH led to the formation of a hitherto unknown SIA‐based dihydro‐1,2‐benzothiazine in quantitative yield.
Detection of distributed denial of service attack using enhanced adaptive deep dilated ensemble with hybrid meta‐heuristic approach
Authors: Aliar Ahamed Ali Samsu , Gowri V. , Abins A. Arockia 
Published: January 2024

AbstractThe biggest firms throughout the world now are the ones that offer services in the cloud. One of the top problems for cloud users (CUs) and cloud service providers (CSPs) is the availability of cloud‐based services whenever needed. A distributed denial of service (DDoS) assault has been a significant threat to the security of the system in recent years. DDoS defense and detection of these attacks have become hot topics of research for academia and business. However, most approaches cannot accomplish efficient detection outcomes with few false alarms. As a result, minimizing the consequences of DDoS attacks allows CSPs to offer CUs high‐quality services. In the cloud sector, a collective deep structured algorithm is suggested to identify DDoS attacks successfully. The recommended method contains several stages: data acquisition, pre‐processing, optimal feature detection, and selection. The first step is the acquisition of data using the help of publicly available sources. Further, the input data undergoes preprocessing. Consequently, the optimal weighted feature selection takes place on the preprocessed data, where the optimization is done with the aid of the Hybrid Border Collie and Dragonfly Algorithm (HBCDA). Finally, DDoS attack detection is achieved via a novel method known as adaptive deep dilated ensemble (ADDE), which includes, one‐dimensional convolutional neural network (1DCNN), deep temporal convolutional neural network (DTCNN), recurrent neural network (RNN), and bidirectional long short‐term memory (Bi‐LSTM). For the attainment of optimal results, the parameter tuning is accomplished by using the HBCDA approach. The detection outcome is computed by using the fuzzy ranking mechanism. The validation is done for the suggested method model, and its corresponding findings are validated with conventional techniques. Hence, the suggested approach outperforms the detection performance and ensures more efficiency than traditional approaches.