bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNF126-mediated ubiquitination of FSP1 affects its subcellular localization and ferroptosis
Authors: Ma Jie , Xie Wanqun , Wang Jiajia , Tian Shuaiwei , Zhao Heng , cao liangliang , Liang Zhuangzhuang , Yang Jian , Zhao Yang , Wang Baocheng , Jiang Feng 
Published: November 2023

Abstract
Medulloblastoma (MB) is a prevalent malignant brain tumor among children, which can be classified into four primary molecular subgroups. Group 3 MB (G3-MB) is known to be highly aggressive and associated with a poor prognosis, necessitating the development of novel and effective therapeutic interventions. Ferroptosis, a regulated form of cell death induced by lipid peroxidation, has been identified as a natural tumor suppression mechanism in various cancers. Nevertheless, the potential role of ferroptosis in the treatment of G3-MB remains unexplored. In this study, we demonstrate that RNF126 acts as an anti-ferroptotic gene by interacting with ferroptosis suppressor protein 1 (FSP1, also known as AIFM2) and ubiquitinating FSP1 at the 4KR-2 sites. Additionally, the deletion of RNF126 reduces the subcellular localization of FSP1 in the plasma membrane, resulting in an increase in the CoQ/CoQH2 ratio in G3-MB. The RNF126-FSP1-CoQ10 pathway plays a pivotal role in suppressing phospholipid peroxidation and ferroptosis both in vivo and in vitro. Our findings indicate that RNF126 regulates G3-MB sensitivity to ferroptosis by ubiquitinating FSP1, which provides new evidence for the potential G3-MB therapy.
Financial difficulties experienced by patients with gastrointestinal stromal tumours (GIST) in the Netherlands: data from a cross-sectional multicenter study
Authors: de Wal Deborah van , Hollander Dide den , Desar Ingrid M. E. , Gelderblom Hans , Oosten Astrid W. , Reyners Anna K. L. , Steeghs Neeltje , Husson Olga , Graaf Winette T. A. van der 
Published: November 2023

Abstract
Purpose
This study aims to (1) explore the prevalence of patient-reported financial difficulties among GIST patients, differentiating between those currently undergoing tyrosine kinase inhibitor (TKI) treatment and those who are not, (2) investigate associations between financial difficulties and sociodemographic and clinical characteristics, work, cancer-related concerns, anxiety and depression, and (3) study the impact of financial difficulties on health-related quality of life.
Methods
A cross-sectional study was conducted among Dutch GIST patients diagnosed between 2008 and 2018, who were invited to complete a one-time survey between September 2020 and June 2021. Patients completed nine items of the EORTC item bank regarding financial difficulties, seven work-related questions, the Hospital Anxiety and Depression Scale, Cancer Worry Scale and EORTC QLQ-C30.
Results
In total, 328 GIST patients participated (response rate 63.0%), of which 110 (33.8%) were on TKI treatment. Patients currently treated with TKIs reported significantly more financial difficulties compared to patients not on TKIs (17.3% vs 8.7%, p = 0.03). The odds of experiencing financial difficulties was 18.9 (95%CI 1.7-214.7; p = 0.02) times higher in patients who were less able to work due to their GIST diagnosis. Patients who experienced financial difficulties had significantly lower global quality of life and functioning, and more frequently reported psychological symptoms as compared to patients who did not report financial difficulties.
Conclusion
Even in a country where the costs of TKIs and follow-up care are covered by health insurance, financial difficulties can be present in GIST patients, especially in patients on TKI treatment, and may negatively influence quality of life.
Ultra-diluted complex additive in the diet reveals benefits in the intestinal tract of nursery-phase piglets
Authors: de Paula Gustavo Zigovski , Bez Isabela Cristina Colaço , Garrido Luís Fernando Costa , Rodrigues Caroline Cintra , de Oliveira Angela Cristina Fonseca , Rupolo Paulo Evaristo , Azevedo Liliana Bury , Hernandez Elmer Modesto Elvir , Genova Jansller Luiz , Weber Saulo Henrique , Carvalho Paulo Levi de Oliveira , Costa Leandro Batista 
Published: November 2023

Abstract
This study aimed to assess an ultra-diluted (UD) complex as a replacement for an antimicrobial performance enhancer in diets on the growth performance, intestinal health, and inflammatory response of nursery piglets. The experiment lasted 37 d and involved 126 animals weaned at 21±1.3 d, with an initial body weight of 5.62±1.16 kg. Piglets were assigned to 6 dietary treatments in a randomized block design with 7 replicates and 3 piglets per pen as experimental unit. The treatments were: positive control – basal diet + 120 mg/kg of chlorohydroxyquinoline (PC); negative control – basal diet without additives (NC); and NC containing 4.5; 6.0; 7.5 or 9.0 kg of UD additive/ton diet. Performance data were calculated, and daily diarrhea occurrence was observed. Blood was collected for hematological analyses. In the end of experiment, one animal per pen was slaughtered for organ weighing, pH analysis and collection of intestinal samples for histopathology. Feces were collected during experiment, and cecal contents at slaughter for microbiological and antibiogram analysis. There was no difference between treatments on performance. Throughout the study, UD levels were equal to PC regarding diarrhea occurrence. Higher levels of UD complex led to higher values of total leukocytes. The 4.5 treatment showed a reduction in total and thermotolerant enterobacteria populations in piglet feces, and an increase in lactic acid bacteria, compared to PC. All treatments showed less duodenal pathological alterations compared to NC. The use of the UD additive, especially at 4.5 kg/ton, may be a good alternative for replacing chlorohydroxyquinoline in piglet diets.
Microstructure and wear resistance of in-situ TiC-reinforced low chromium iron-based hardfacing alloys
Authors: Tong Zhixiang , Shao Wei , He Chengxing , He Dingyong 
Published: November 2023

Abstract
Strengthening low chromium iron-based alloys with multiple alloy elements is a crucial strategy for developing "casing-friendly" hardbanding materials. In this paper, four kinds of low chromium iron-based hardfacing alloys with different Ti contents were prepared by Flux-Cored Arc Welding technology and the effect of Ti content on the microstructure, hardness and wear performance under the abrasive wear and dry sliding wear were analyzed. The in-situ TiC-reinforced iron-based hardfacing alloys exhibited microstructure comprising austenite, martensite, eutectic carbides M7C3 and in-situ TiC particles. The in-situ formation of TiC particles consumed the carbon in the alloy and thus raised the martensite start temperature, resulting in a reduction in the mass fraction of austenite and eutectic carbides M7C3 and an increase in the martensite. Among four hardfacing alloys, the alloy with 5 wt. % Ti exhibited the highest hardness (836.3 HV0.2), the lowest weight loss and the lowest wear rate due to its highest martensite mass fraction (69.97 wt. %). The matrix with in-situ TiC particles and martensite effectively resisted the cutting of abrasive grains and the wear mechanism developed from the microploughing in 0Ti alloy to microcutting in in-situ TiC-reinforced iron-based hardfacing alloys in abrasive wear tests. In dry sliding wear tests, TiC particles were released and slid on the surface of the alloys, resulting in the formation of grooves, while the formation of the tribochemical reaction layers contributed to a reduction in the friction coefficient and wear rate. This study provides a theoretical foundation for the development of "casing-friendly" hardbanding materials.
Preventive and therapeutic potential of Streptococcus cristatus CA119 in experimental periodontitis in rats
Authors: Zhao Dongyu , 李 明 , Pan Tianxu , Guo Jialin , Shi Chunwei , Wang Nan , Huang Haibin , Wang Chunfeng , Yang Guilian L. , Li Junyi 
Published: November 2023

Abstract
Periodontitis is a chronic inflammatory disease characterized by the overgrowth of Porphyromonas gingivalis and Fusobacterium nucleatum in the oral cavity. This not only severely affects alveolar bone and connective tissues but also has potential associations with distal intestinal inflammation. This study aimed to elucidate the therapeutic effects of Streptococcus cristatus CA119 on experimental periodontitis in rats and its impact on intestinal morphology. The results demonstrate that CA119 can colonize the oral cavity and exerts antagonistic effects on P. gingivalis and F. nucleatum, leading to a significant reduction in the oral pathogen load. Following CA119 intervention, a significant alleviation in weight loss in rats induced by periodontitis was observed (P < 0.001). CA119 also regulated the expression of IL-6 (P < 0.05), IL-1β (P < 0.001), IL-18 (P < 0.001), COX-2 (P < 0.001), iNOS (P < 0.001), and MCP-1 (P < 0.01) in the gingival tissue. Additionally, CA119 reduced oxidative stress levels in rats and enhanced their antioxidant capacity. Microcomputed tomography (micro-CT) and histological analysis revealed that CA119 significantly reduced alveolar bone loss and reversed the down-regulation of OPG/RANK (P < 0.001). Furthermore, CA119 exhibits a significant protective effect against intestinal inflammation induced by periodontal disease and improves the colonic morphology in rats. In conclusion, this study demonstrates the role of CA119, a potential oral probiotic, in preventing and treating experimental periodontitis, which underscores the potential of probiotics as a complementary approach to traditional periodontal care.
Routine magnetic resonance cholangiopancreatography before laparoscopic cholecystectomy in patients with cholecystolithiasis
Authors: Guo Xu , Fan Qing , Wang Zhuoyin , Wang Jing , Li Kai , Xu Guangzhong , Du Dexiao , Lian Dongbo , Zhang Nengwei , Amin Buhe , Zhu Bin 
Published: November 2023

Abstract
Objective
To demonstrate if patients with cholecystolithiasis at low risk of common bile duct stones (CBDS) require magnetic resonance cholangiopancreatography (MRCP) before laparoscopic cholecystectomy (LC). This study investigated the clinical value of routine MRCP before LC in patients with gallstones.
Methods
We adopted a non-randomized controlled experiment according to whether MRCP performed prior to LC, 277 patients with cholecystolithiasis at low risk of CBDS were divided into two groups:142 patients in the control group only underwent abdominal ultrasound, while 135 patients in the experimental group underwent both abdominal ultrasound and MRCP. The results of laboratory test, abdominal ultrasound and MRCP, surgical complications, length of stay and hospitalization costs were compared between the two groups.
Result
The detection rates of CBDS, cystic duct stones, gallbladder adenomyomatosis and anatomical variants of biliary tract by preoperative MRCP were higher than those by abdominal ultrasound (P < 0.05). The incidence of postoperative complications in the experimental group was significantly lower than that in the control group (0% vs. 5.63%, P < 0.05). The length of stay in the experimental group was shorter than that in the control group (P < 0.05).
Conclusion
Routine MRCP before LC can detect CBDS, cystic duct stones and anatomical variants of biliary tract that cannot be diagnosed by ultrasound. MRCP helps surgeons better understand the anatomical variation of extrahepatic bile duct, plan the operation rationally and efficiently reduce the occurrence of postoperative complications. Moreover, MRCP shorten the length of stay without increasing the hospitalization costs.
A Paradigm Based on Big Data for Smart and Sustainable Additive Manufacturing
Authors: Vekariya Vipul , Rao Raghavendra , Thakuriya Kajal , Saxena Kumud , Alam Intekhab , Dandotiya Devendra Dandotiya Devendra 
Published: November 2023

Abstract
Due to a decrease in material and resource usage as well as other tooling needs, additive manufacturing (AM) has shown significant promise for energy-saving and environmentally friendly production. As a consequence of improvements in manufacturing technology, academia, and business are becoming more interested in smart manufacturing to help make their operations more sustainable and efficient. The term smart and sustainable additive manufacturing (SSAM) is created in this study by combining the core concepts of AM and SSAM. The study aims to give a framework that aids AM businesses by integrating big data analytics (BDA), AM, and sustainable smart manufacturing technologies. A framework of big data-driven SSAM (BD-SSAM) is designed to help executives in the AM sector make wiser decisions throughout the product life cycle beginning of life (BOL) stage. To illustrate the suggested approach, a sample application case from the field of additive manufacturing was given. Selective laser melting (SLM) is being used to implement the proposed design for the AlSi10Mg alloy due to resource limitations and the requirement to create components made of the BOL stage of the product lifecycle. The results demonstrate the effective management of energy utilization and product quality, which benefits efficient sustainable manufacturing, a drop in emissions, and cleaner production.
Laparoscopic Training Workshop to Assess Medical Students’ Skill Acquisition and Interest in Surgical Careers
Authors: Chen Pin-Chun , Yang Po-Wen , Kao Yi-Kai , Chen Chia-Hung , Tsai Chih-Jong , Chen Yi-Chieh , Song Ling-Chiao , Tsai Kai Lung , Wu Richard C. , Chen Chih-I 
Published: November 2023

Abstract
Background
With its minimally invasive approach, laparoscopic surgery has transformed the medical landscape. As the demand for these procedures escalates, there is a pressing need for adept surgeons trained in laparoscopic techniques. However, current training often falls short of catering to medical school education. This study evaluates the impact of a custom-designed laparoscopic training workshop on medical students’ surgical skills and career aspirations.
Methods
This prospective experimental study was conducted at the E-Da hospital in Kaohsiung City, Taiwan. Medical students from Taiwanese medical schools undergoing Clerk 5, Clerk 6, and Postgraduate Year 1 and 2 were invited to participate. Medical students (n = 44) underwent an endoscopic skill training workshop consisting of lectures, box training, and live tissue training. The trainees performed multiple tasks before and after training using our objective evaluation system. The primary outcome was gauged through a questionnaire assessing the influence of training on students’ interest in surgery as a career. Secondary outcomes measured improvements in skill acquisition, comparing task completion times pre- and post-workshop.
Results
Post-training, participants exhibited significant proficiency gains, with task completion times reducing notably: 97 s (p = 0.0015) for Precision Beads Placement, 88.5 s (p < 0.0001) for Beads Transfer Exercise, 95 s (p < 0.0001) for Precision Balloon Cutting, and 137.8 s (p < 0.0001) for Intracorporeal Suture. The primary outcome showcased an increased mean score from 8.15 pre-workshop to 9.3 post-workshop, indicating a bolstered interest in surgery as a career. Additionally, post-training sentiment analysis underscored a predominant inclination toward surgery among 88% of participants.
Conclusion
The custom-designed laparoscopic workshop significantly improved technical skills and positively influenced students’ career aspirations toward surgery. Such hands-on training workshops can play a crucial role in medical education, bridging the gap between theoretical knowledge and practical skills and potentially shaping the future of budding medical professionals.
The Impact of Chronic Antibiotics Exposure on the Gut Microbiome Dysbiosis and Mucosal Barrier-Related Pathogen Susceptibility in Zebrafish Model
Authors: Yang Jun Hyeok , Park Jeong Woo , Lee Seungki , Yerke Aaron M. , Jaiswal Yogini S. , Williams Leonard L. , Hwang Sungmin , Moon Ki Hwan 
Published: November 2023

Abstract
The symbiotic community of microorganisms in the gut plays an important role in the health of the host. While many previous studies have been performed on the interaction between the gut microbiome and the host in mammals, studies in fish are still lacking. In this study, we investigated changes in the intestinal microbiome and pathogen susceptibility of zebrafish (Danio rerio) following chronic antibiotics exposure. The chronic antibiotics exposure assay was performed on zebrafish for 30 days using oxytetracycline (Otc), sulfamethoxazole/trimethoprim (Smx/Tmp), and erythromycin (Ery), which are antibiotics widely used in aquaculture industry. The microbiome analysis indicated that Fusobacteria, Proteobacteria, Firmicutes, and Bacteroidetes were the dominant phyla in the gut microbiome of zebrafish used in this study. However, in Smx/Tmp-treated zebrafish, the composition of Fusobacteria and Proteobacteria were changed significantly, and in Ery-treated zebrafish, the composition of Proteobacteria and Firmicutes were altered significantly. Although alpha diversity analysis showed that there was no significant difference in the richness, beta diversity analysis revealed a community imbalance in the gut microbiome of all chronically antibiotics exposed zebrafish. Intriguingly, in zebrafish with dysbiosis on the gut microbiome, the pathogen susceptibility to Edwardsiella piscicida, a representative Gram-negative fish pathogen, was reduced. Due to the further effect of gut microbiome dysbiosis, the number of goblet cells in the intestinal tissue was increased, and the intestinal mucosal barrier-related genes expression was also upregulated. In addition, as the innate immunity was enhanced by the increased mucosal barrier, the immune, stress-related gene expression in the intestinal tissue was downregulated. In this study, we provide new insight into the effect of gut microbiome dysbiosis on pathogen susceptibility.
Prevalence and factors associated with mental health problems among undergraduates in a Nigerian university
Authors: Orok Edidiong , Okuoru Woyengikuro , Oni Olawale , Ojo Tolulope , Oluwadare Tunrayo , Bamitale Toba , Jaiyesimi Boluwaji , Akinjisola Alice , Apara Titilayo 
Published: November 2023

Abstract
Background
The study aimed to investigate the prevalence of mental health problems (MP) among undergraduate students at Afe Babalola University, Ado-Ekiti (ABUAD). It also sought to identify the factors associated with MP among these students.
Methods
A cross-sectional research design was employed, utilizing a self-administered, semi-structured questionnaire. Logistic regression was used to analyse the relationship between various factors and MP. Analysis was carried out using Statistical Package for Social sciences (SPSS)
Results
A total of 354 students participated in the study, with an average age of 18.51 years (± 1.741), and a majority of male respondents (52.0%). The study found that the prevalence of MP among ABUAD students was 9.6%. Among the common mental health problems identified, depression was the most prevalent at 32.4%, followed by anxiety at 14.7%, and schizophrenia at 8.8%. Furthermore, the study revealed that the female gender was more likely to receive a diagnosis of MP compared to males (p = 0.002; OR: 6.574, 95% CI: 1.992–21.692). Conversely, students who had strong social support were less likely to be diagnosed with MP than those without such support (p = 0.022; OR: 0.225, 95% CI: 0.433–1.738). Additionally, students who missed a significant number of classes during the semester were more likely to receive a diagnosis of MP compared to those who did not (p < 0.001; OR: 11.030, 95% CI: 2.877–42.287).
Conclusion
This study indicated a relatively low overall prevalence of MP among ABUAD students. Factors such as gender, social support, and frequent class absences were identified as contributors to an increased likelihood of MP among these students. The study underscores the need for interventions aimed at reducing the prevalence of MP among ABUAD students.
Traceguard: Eradicating Counterfeits With Blockchain Transparency
Authors: N Moratanch , Sre Nivetha , C Lavanya , S Mamtha , D Nivethaa 
Published: November 2023

Abstract

Counterfeit products pose a significant threat to various industries, impacting company reputations, sales, and profits. In response to this challenge, the integration of blockchain technology and Quick Response (QR) codes has emerged as a robust solution. This innovative approach enhances product authentication and traceability while reducing reliance on third-party intermediaries for verification. By assigning unique QR codes linked to blockchain records, customers can easily verify product authenticity, and companies can provide transparency throughout the supply chain. The decentralized and immutable nature of blockchain ensures data security, while real-time updates and cost-efficiency add to the appeal of this solution.
Transfer of heavy metals from soil to Beta vulgaris L. (sugar beet) in Kayseri, Turkey
Authors: LEBLEBİCİ Zeliha , BÜLBÜL Hayrun Nisa 
Published: November 2023

Abstract

In our study, sugar beet plants grown in Yeşilhisar district of Kayseri were used. 6 different locations were selected: Kesik, Dağılgan, Hacılımezarlığı, Kıraç Tepe, Hacıbektaş and the control area (Kilcan). Leaf, beet, root and soil samples were taken from Beta vulgaris plant. Heavy metal (As, Cd, Pb, Cu, Ni and Cr) contents were analyzed using ICP-MS device. In terms of sugar beet consumption in Kayseri province, Turkey, determining heavy metal accumulation and health risks will be very important in terms of these metals. Heavy metal concentration levels in sugar beet samples collected from the region are as follows; nickel (Ni) > copper (Cu) > chromium (Cr) > arsenic (As) > lead (Pb) > cadmium (Cd). When As, Cd and Cr elements are compared with the limit values, it is observed that they are within the allowed limits. Cu, Ni and Pb indicate the potential for negative contributions to health through sugar beet consumption. The study reveals health concerns regarding the heavy metal content of sugar beet samples grown in Yeşilhisar, Kayseri province and recommends that necessary measures be taken to reduce the occurrence of Cu, Ni and Pb observed in sugar beet samples from this region.
A rare Presentation of Aberrant Right Subclavian Artery: A Case Report
Authors: AryaNezhad Ali , Salehi Mohamad Sajad , AryaNezhad Yasin 
Published: November 2023

Abstract
The aorta, a vital artery originating from the left ventricle, typically divides into three branches in most individuals: the brachiocephalic trunk, the left common carotid artery, and the left subclavian artery. However, some individuals may present with variations in the branching pattern of the aortic arch. This case report discusses the unusual presentation of an Aberrant Right Subclavian Artery (ARSA) in a 50-year-old woman from Iran who sought medical attention due to dyspnea. Upon admission to the emergency department, the patient's blood pressure measurements in both arms showed a significant difference. This led to further investigation, including a CT angiography, which revealed an unusual course of the right subclavian artery. Instead of following the typical anatomical route, the aberrant artery passed through the second and third ribs, entered the right breast tissue, and then spiraled into the right upper limb. This case not only adds to the body of medical knowledge but also emphasizes the significance of clinical awareness and thorough diagnostic evaluation when encountering unusual and unexpected patient presentations.