bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of human CD8+ T cell senescence on 89Zr radiolabelling and homing properties
Authors: Bystrom Jonas , Carrascal-Miniño Amaia , Keeling George P , Pham Truc , Garrod-Ketchley Conor , Schroth Johannes , De Rosales Rafael T. M. , Terry Samantha YA , Henson Sian M 
Published: November 2023

Abstract
Background
The ability of CD8+ T cells to protect against infection and malignant transformation diminishes with age. Novel means to assess cellular functional deficits in vivo are being made available such as total-body positron emission tomography (PET) and radiotracers with long half-lives. Here, we determined radiolabeled human CD8+ T cells isolated from young and old individuals with zirconium-89 (89Zr) and assessed their biological status in vitro and distribution in vivo.
Results
Fresh and cryopreserved CD8+ T cells showed no difference in ability to be labelled with 89Zr, radionuclide retention, or CD8+ T cell phenotype. 89Zr induced partial cell death and DNA damage, which was no longer detectable visible after four days. The level of DNA repair observed in old samples was highly variable. 89Zr efflux from cells, seen in vitro, did not occur in vivo. Longitudinal PET imaging indicated that CD8+ T cells from old individuals accumulated in tissues at a slower rate than those isolated from young individuals.
Conclusion
We have established a strategy to label and track the biodistribution of cryopreserved CD8+T cells. Further study is required to understand differences in migratory behaviour of CD8+ T cells isolated from old and young individuals.
Serum 25-hydroxyvitamin D and nasopharyngeal carcinoma risk and survival: a hospital-based matched prospective case-control study in Malaysia
Authors: Ulaganathan Vaidehi , Lye Munn-Sann , Al-Olayan Ebtesam , Loh Su-Peng , Yap Yoke-Yeow , Kandiah Mirnalini , Augundhooa Digsha , Gunasekaran Baskaran 
Published: November 2023

Abstract
Serum 25(OH) D deficiency may be one of the underlying molecular mechanisms through which chronic inflammation is associated with risk and survival of nasopharyngeal carcinoma (NPC). This study was conducted to determine the association between serum 25(OH) D and NPC risk and survival. A multi-centric case-control study was conducted from two local hospitals. A total of 300 histologically confirmed NPC cases were matched with controls for age, gender and ethnicity and assessed for vitamin D status and other factors. Mean serum 25(OH) D was significantly lower in NPC patients compared with controls (25.3 ± 7.7 ng/mL vs. 27.0 ± 9.2 ng/mL, p = 0.016). Multiple logistic regression analysis indicated that higher levels of serum 25(OH) D was associated with reduced odds of NPC (AOR = 0.73, 95% CI = 0.57–0.94) controlling for confounders including BMI, physical activity, smoking status, alcohol consumption, consumption of food high in vitamin D, salted fish consumption and family history of NPC. There was a significant association between insufficient serum 25 (OH) D status with accumulation of four risk factors and increased odds of getting NPC using fractional polynomial analysis. Increased NPC odds was observed after accumulation of second risk factors with presence insufficient serum 25 (OH) D status (OR = 0.54, 95% CI = 0.27, 4.77, OR = 1.04, 95% CI = 0.64, 1.72, OR = 1.15, 95% CI = 0.73, 1.80, OR = 1.93, 95% CI = 1.13, 3.31, and OR = 5.55, 95% CI = 1.67, 10.3 respectively). The 5-year survival rates were 51.0%, 57.0% and 43.0% for serum 25(OH) D deficiency, insufficiency and sufficient respectively. These findings highlight the potential preventive benefits of maintaining higher levels of serum 25(OH) D in mitigating NPC risk, though further research is needed to explore its impact on survival outcomes.
Skeletal muscle-specific prolyl hydroxylase-2 deficient mice release more lactate into the blood during acute exercise and have greater endurance running capacity
Authors: Kubota Takahiro , Takada Hiroaki , Osana Shion , Nunomiya Aki , Nagatomi Ryoichi 
Published: November 2023

Abstract
Prolyl hydroxylase domain 2 (PHD2) deficiency has been shown to activate hypoxia inducible factor (HIF), which improves the effects of endurance exercise training. In previous research, although systemic knockout of Phd2 causes an increase in blood hematocrit levels, only knockout Phd2 does not cause an increase in exercise capacity. At the time when the endurance of Phd2 knockout mice was significantly increased by post-training, an increase in slow muscle fibers in skeletal muscle and metabolic changes were observed. Thus, the contribution of hypoxic response in skeletal muscle is likely to be significant. Since the hypoxic response occurs in various organs and tissues throughout the body, a model in which the hypoxic response occurs only in skeletal muscle is needed to find the cause of the improved effects of hypoxic training in skeletal muscle. We therefore generated mice genetically modified to lack Phd2 specifically in skeletal muscle (Phd2mKO mice) and measured their endurance exercise capacity. We found that Phd2mKO mice ran 13.3% longer than control mice in an endurance running test. Furthermore, the number of contractions before the muscle contractility decayed by half was increased by 26% in the measurement of muscle endurance by forced muscle contraction of the gastrocnemius muscle by electrical stimulation. Furthermore, acute exercise increased blood lactate levels by 56% and showed differential expression of the metabolism-related genes GLUT1, GLUT4 MCT1 MCT4 in skeletal muscle. This suggests that the local hypoxic response in skeletal muscle improves endurance exercise capacity without changes in blood hematocrit levels due to altered expression of metabolism-related genes.
Optimization of nonlinear equations with constraints using genetic algorithm
Authors: Čajić phd can sc Elvir 
Published: November 2023

Abstract
This scientific paper investigates the effectiveness of applying the genetic algorithm in solving nonlinear equations with set constraints. A genetic algorithm is used as an optimization method to find a solution to a system of equations that satisfies a given set of constraints. The paper analyzes the performance of the genetic algorithm in the context of solving non-linear problems, providing insight into its applicability and advantages compared to other methods.
Natural Plant Compounds as ANGPTL2 Inhibitors in Glioblastoma: An Integrated Bioinformatics and Molecular Docking Analysis
Authors: POLEBOYINA PAVAN KUMAR , Poleboyina Sneha Malleswari , Pawar Smita 
Published: November 2023

Abstract
Glioblastoma is a primary brain tumor notorious for its extreme aggressiveness and inevitability of recurrence, resulting in a dire prognosis. Angiopoietin-like proteins comprise eight glycoproteins that are secreted and share structural similarities with angiopoietin family proteins. The researchers discovered that the expression of ANGPTL2 is significantly elevated in GBM tumors and that this elevation correlates with the proliferation and dissemination of glioma cells. In drug discovery, Natural plant compounds derived from plants have shown properties that can inhibit the activity of cancer cells. These compounds are capable of inhibiting cancer cell proliferation and promoting apoptosis. In our study, we conducted an integrated bioinformatics analysis to investigate the role of ANGPTL2 in GBM. We utilized natural plant compound inhibitors to identify the plausible inhibitor targeting ANGPTL2. Due to the unavailability of the 3D structure of ANGPTL2 in the Protein Data Bank, we utilized homology modeling using I-TASSER to create a 3D protein model of ANGPTL2. Molecular docking studies were conducted and the highest-scoring ligand was identified. Multiple parameters were utilized in the analysis of protein-ligand interactions. According to our findings, 22-epicalamistrin is a promising potential ANGPTL2 inhibitor for reducing ANGPTL2 expression and halting the progression of Gliomas.
Intention to Utilize Mental Health and Suicide Prevention Resources in a Community Sample During the First Year of the COVID-19 Pandemic
Authors: Cohen Lisa , Hayek Rawad El , Imbastaro Benedetta , Goncearenco Inna , Zheng Sifan , Rogers Megan L. , Pompili Maurizio , Galynker Igor 
Published: November 2023

Abstract

Objective
Given the stressors experienced during the COVID-19 pandemic, it is critical to identify populations with elevated mental health needs during this crisis. This study investigated demographic correlates of reported intention to utilize mental health (MH) and suicide prevention (SP) resources in a community sample during the COVID-19 pandemic.
Methods
A sample of 1,978 adults in the United States completed an anonymous online survey between June 2020 and February 2021.
Results
Intent to utilize MH resources was associated with younger age, single marital status, female gender, and Hispanic vs. White race/ethnicity. Intent to utilize SP resources was associated with younger age, single marital status, and was greater among Black and Hispanic vs. White race/ethnicity. Lower education was associated with MH and SP utilizers in bivariate analysis. Indirect effects of Suicide Crisis Syndrome (SCS) symptoms were found on the association of age, gender, and marital status with MH utilization and of age, marital status, and education with SP Utilization.
Conclusions
Specific demographic populations demonstrate greater interest in mental health care during the COVID-19 pandemic. These help-seeking patterns can be explained in part by an elevated level of SCS symptoms, suggesting greater levels of distress were driving expressed intention to utilize service referrals.
Novel fabricated chitosan supported bimetallic nanocomposites for paracetamol removal from homogeneous solutions and heterogeneous mixtures with focused antibacterial studies
Authors: Priyadarshi Gautam V. , Raval Nirav P. , Barcelo Damia , Trivedi Mrugesh H. 
Published: November 2023

Abstract
Biopolymers infused with bimetallic nanoparticles exhibit a wide range of functionalities necessary for efficiently eliminating diverse water contaminants. However, the protracted production process requires further exploration. As such, this current study seeks to optimize microwave-assisted technique for the facile synthesis of cross-linked chitosan (CTS) supported bimetallic oxide nanoparticles, specifically zinc oxide (ZnO) and iron oxide (Fe3O4), denoted as CTS-TTP/Zn-Fe. The primary objective is to investigate the efficacy of these beads in the removal of Paracetamol (PCM) from single and complex water matrices while also assessing their antibacterial properties. Characterization includes chemical composition, surface structures, thermal stability, and magnetic properties. The experimental results demonstrated that CTS-TPP/Zn-Fe beads achieved a remarkable PCM removal efficiency of approximately 99%, with a Zn:Fe mole ratio of 1:1. The experimental data showed good applicability with Freundlich isotherm and chemisorption-supported rate models (R2>0.9). To evaluate the long-term viability and practicality of these beads, three crucial field applicability tests were conducted. These encompassed competition studies with other pharmaceuticals, desorption investigations for repeated use, and efficiency evaluations in an ionic solution. Collectively, this research provides a comprehensive understanding, spanning from material design to practical applications, with potential relevance for large-scale wastewater treatment when coupled with appropriate flux control measures.
Modulating Leidenfrost-like Prompt Jumping of Sessile Droplets on Microstructured Surfaces
Authors: Cheng Jiangtao , Huang Wenge , Zhao Lei , Li Yang , He Xukun , Collier C. , Zheng Zheng , Liu Jiansheng 
Published: November 2023

Abstract

The Leidenfrost effect, namely the levitation and hovering of liquid drops on hot solid surfaces, generally requires a sufficiently high substrate temperature to activate the intense liquid vaporization. Here we report the agile modulations of Leidenfrost-like prompt jumping of sessile water microdroplets on micropillared surfaces at a remarkably mitigated temperature. Compared to traditional Leidenfrost effect occurring above 230 °C, the fin-array-like micropillars enables Wenzel-state water microdroplets to levitate and jump off within 1.33 ms at an unprecedently low temperature of 130 °C by triggering the inertia-controlled growth of individual vapor bubbles at the droplet base. We demonstrate that droplet jumping, resulting from the momentum interactions between the expanding vapor bubble and the droplet, can be deftly modulated by simply tailoring the thermal boundary layer thickness via pillar heights, which acts to regulate the bubble expansion between the inertia-controlled mode and the heat-transfer-limited mode. Intriguingly, the two bubble growth modes give rise to distinct droplet jumping behaviors characterized by constant velocity and constant energy schemes, respectively. This heating strategy allows the facile purging of wetting liquid drops on rough or structured surfaces in a controlled manner, inspiring promising applications in rapid removal of fouling even settled in surface cavities.
Structure and electrocatalytical properties of electrodeposited M-Ir (M=Co, Ni) bimetallic alloy catalysts with low Ir loading obtained on copper foams for hydrogen evolution reaction
Authors: Zhou Yicheng , Ju Liu , Yang Yang , wu wangping 
Published: November 2023

Abstract

M-iridium (M = cobalt (Co), nickel (Ni)) bimetallic alloy catalysts with low iridium (Ir) loading of 0.3 ~ 2.0 mg·cm− 2 were prepared on copper foam (CF) supports by electrodeposition. The top surface of as-deposited M-Ir catalysts was mainly composed of metallic state and oxides states, such as metallic Ir, Ni(OH)2 or Co(OH)2, Co(Ir) and Ni(Ir) solid solution, Ir oxides. M-Ir catalysts with low Ir loading exhibited excellent catalytic performance. Ni67.4Ir32.6/CF catalyst with low Ir loading of 2.0 mg·cm− 2 achieved a current density of 10 mA·cm2 at an overpotential of 52 mV and a Tafel slope of 36 mV·dec− 1. Co64.2Ir35.8/CF catalyst with low Ir loading of 0.7 mg·cm− 2 was uniformly scattered with small ellipsoidal particles, looking like fine fluff, requiring an overpotential of 51 mV for hydrogen evolution reaction to reach a current density of 10 mA·cm− 2, having a Tafel slope of 38 mV·dec− 1. After long-term hydrogen evolution testing, M-Ir/CF catalysts exhibited excellent electrocatalytic stability for water splitting in alkaline solution.
Enlarged Perivascular Space in the Basal Ganglia Associated with Coexisting Intracerebral Hemorrhage in Patients with Recent Small Subcortical
Authors: wu zhengrong , Zong Ce , Zhang Ke , Liu Hongbing , Wang Yanhong , Xu Yuming , Gao Yuan 
Published: November 2023

Abstract
Background: Intracerebral hemorrhage (ICH) is a common vascular disease associated with small blood vessels, and it is not uncommon for individuals with cerebral small vessel disease (CSVD) to develop ICH. Studies have shown that the number and location of cerebral microbleeds (CMBs) are associated with ICH in individuals with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). However, the prevalence of ICH and its related risk factors in individuals with recent subcortical small infarct (RSSI) is still unclear.
Methods: This study included 361 patients with complete MRI data. ICH was defined based on the medical history of cerebral hemorrhage and brain MRI and SWI scans, which are sensitive for detecting both old and recent hemorrhages. The location and quantity of enlarged perivascular spaces (EPVS) were assessed using a visual scoring scale. Additionally, evaluation was performed for CMBs,cerebral white matter hyperintensities (HWMHs)and lacunar infarctions(LI). The correlation between ICH and EPVS was analyzed using multivariate logistic regression analysis.
Results: The study ultimately included 361 patients with RSSI in the lenticulostriate arterial region, with a mean age of 59.70±12.11 years. Among them, 243 (67.3%) were male, and 34 (9.4%) had concomitant ICH. In univariate analysis, the proportion of patients with a history of cerebral infarction was higher in the ICH group, platelet count was lower, and there were higher proportions of basal ganglia perivascular spaces (BG-HEPVS),CMBs,deep/mixed CMBs,HCMBs (high CMBs),high white matter hyperintensities (HWMHs), and LI. The median CSVD total burden score was also higher in the ICH group.Multivariable logistic regression analysis showed that in Model 1, BG-HEPVS(OR, 4.12; 95% CI, 1.34-12.70; P=0.014) and deep/mixed CMBs (OR, 5.62; 95% CI, 1.50-21.03; P=0.010) were significantly associated with ICH. In Model 2, the total burden of CSVD (OR, 2.26; 95% CI, 1.54-3.33; P<0.001) was significantly associated with ICH.
Conclusion: In the population with RSSI, BG-EPVS, deep/mixed CMBs and the total burden of CSVD were significantly associated with ICH. The strength of this association and the ability to identify the risk of bleeding in clinical practice may be useful for the safety of future antithrombotic treatments.
Evaluating the AllplexTM GI-Bacteria (I) Assay Efficiency to Assess the Diversity of Bacterial Pathogens among Young Children with Diarrhea in Burundi
Authors: Ihorimbere Theogene , Nzoyikorera Néhémie , Gehre Florian , Sangare Modibo , Kezakarayagwa Eric , Kabatesi Francine , Nduwimana Cassien , Ndihokubwayo Armstrong , Nibogora Celestin , Affara Muna , Molina Andrea , Idris Hakim Lagu , Achol Emmanuel , Nyandwi Joseph 
Published: November 2023

Abstract
Background
Diarrheal infections are caused by many enteric pathogens that infect children as early as birth. Molecular assays are uncommon in routine diagnosis of gastrointestinal bacterial pathogens in Burundi. This study aimed to characterize the diversity of bacterial pathogens among young children with diarrhea in selected healthcare settings and to demonstrate the efficiency of the AllplexTM GI-Bacteria (I) assay over the conventional culture.
Methods
We conducted a cross-sectional study in seven (7) selected health districts in Burundi. We used a questionnaire to collect sociodemographic information for children ≥ 5 years old whose parents consented to participate. We collected stool samples for both conventional cultures (gold standard) and AllplexTM GI assay. We plated the stool samples shortly after collection and we kept the samples in the fridge between 4–8 ℃ for less than 24 hours before we processed them with the AllplexTM GI kit. We determined the association between sociodemographic factors and carriage of genes using a chi-square test for independence (Pearson's chi-square test or chi-square test of association). A p-value < 0.05 was considered statistically significant.
Results
17.6% (18/102) of stool specimens processed with conventional methods were positive for Shigella spp. (13.7%) and Salmonella spp (3.9%). With Allplex GI, 79.4% (81/102) of stool samples were positive for different genes; sh/ei genes were detected in 40.2% (40/102) of the stool samples. An association of two genes namely sh/ei and sal or sh/ei and vib was found. AllplexTM GI-Bacteria(I) PCR Assay had a sensitivity of 94.4% and a negative predictive value (NPV) of 95.2%. There was no association between place of residence, fingernail status, level of education, and being positive for sh/ei, sal, sh/ei and sal and sh/ei and vib.
Conclusion
We characterized bacterial pathogens in the stool samples and we demonstrated the efficiency of the AllplexTM GI assay over conventional methods. The data could further inform the decision-makers to institute effective prevention and control measures to tackle infectious diarrhea.
Investigating Antialzheimer Potential of Biogenic Compounds From Zinc 15 Database As NMDA Antagonist: A Insilico Approach.
Authors: Chaudhari Somdatta Y. , Shinde Asavari R. , Madar Pratiksha R. , Shingan Aarti Vijayakumar , Bairagi Shriram Hiradas , Dhage Azad Nabilal , Patel Pinkal , Rathod Vivek Digamber , Jadhav Shailaja B. , Chaudhari Pravin D. 
Published: November 2023

Abstract
Alzheimer's disease is an unavoidable neurological disorder in which memory loss, cognitive decline, and eventual dementia are brought on by the death of brain cells. There is no recognized treatment for Alzheimer's illness. By the year 2050, it is expected that the global population will witness approximately 100 million cases of Alzheimer’s disease (AD). Despite recognizing AD as a formidable illness for over a century, no effective cure has been discovered thus far. Synaptic dysfunction could result from disturbed synaptic calcium handling caused by excessive activation of glutamate receptors, particularly the N-methyl-D-aspartate receptors (NMDARs). Glutamate serves as the main excitatory neurotransmitter in the brain, acting on both ionotropic and metabotropic glutamate receptors.
In recent years, a number of pharmacologically active substances that were derived from plants, animals, and microbes have shown promise in the treatment of AD by focusing on various pathogenic processes. Initially, we used virtual screening to assess natural product-like compounds against NMDA receptors. In this research study, we have screened a natural compound database derived from zinc15. The best candidate was then validated through molecular dynamics simulation (MDS). The results revealed that out of 4221 compounds tested, only 165 showed superior binding interactions compared to native ligands, making them inhibitors for protein. Further analysis using ADMET indicates favorable drug-like properties, particularly for CNS drug-likeness. The MDS results, including RMSD, RMSF, Rg, and residue interactions, indicated a strong and stable association between top molecules and target protein. This confirms that top molecules can effectively remain within the binding pockets of the target proteins, forming stable protein-ligand complexes.
Effect of Post-Transplant Diabetes Mellitus on Cardiovascular Events and Mortality: A single‐center retrospective cohort study
Authors: Ünlütürk Uğur , Yıldırım Tolga , Savaş Merve , Oğuz Seda Hanife , Fırlatan Büşra , Yüce Deniz , Karakaplan Nesrin Damla , Selimova Cemile , Yılmaz Rahmi , Erdem Yunus , Bayraktar Miyase 
Published: November 2023

Abstract
Purpose
This study aims to investigate the impact of post-transplant diabetes mellitus (PTDM) on cardiovascular events, graft survival, and mortality and to determine the risk factors involved in developing PTDM.
Methods
A total of 703 patients who underwent kidney transplantation were included in the study. The total sample was subdivided into three groups: (i) patients with PTDM; (ii) patients who had diabetes before the transplantation (DM); and (iii) patients without diabetes (NoDM). The data on graft failure, cardiovascular events, all-cause mortality, and the potential risk factors that play a role in developing PTDM were recorded and analyzed.
Results
The patients were followed for a median of 80 (6-300) months after transplantation. Out of all patients, 41 (5.8%) had DM before transplantation, and 101 (14.4%) developed PTDM. Recipient BMI, post-transplant fasting plasma glucose, and hepatitis C seropositivity were independent risk factors for PTDM development. The incidence of cardiovascular events was 6.1% in the NoDM group, 14.9% in the PTDM group, and 29.3% in the DM group (p < 0.001). In PTDM patients, hepatitis C seropositivity and the recipient's age at transplant were independent predictors of a cardiovascular event. There were no significant differences between the groups regarding the risk of graft loss. PTDM had no significant effect on all-cause mortality. However, the survival rates of DM patients were significantly reduced compared to those with NoDM or PTDM.
Conclusions
PTDM had no impact on patient survival. Hepatitis C seropositivity and recipient age at transplant predicted cardiovascular events in PTDM patients.