bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Type VI Secretion System inBurkholderiaSpeciescenocepaciaandorbicolaTriggers Distinct Macrophage Death Pathways Independent of the Pyrin Inflammasome
Authors: Loeven Nicole A. , Reuven Arianna D. , McGee Abigail P. , Dabi Clarrisa , Mwaura Bethany W. , Bliska James B. 
Published: September 2023

AbstractTheBurkholderia cepaciacomplex contains opportunistic pathogens that cause chronic infections and inflammation in lungs of people with cystic fibrosis. Two closely related species within this complex areBurkholderia cenocepaciaand the recently classifiedBurkholderia orbicola. B. cenocepaciaandB. orbicolaencode a type VI secretion system and the effector TecA, which is detected by the pyrin/caspase-1 inflammasome, and triggers macrophage inflammatory death. In our earlier study the pyrin inflammasome was dispensable for lung inflammation in mice infected withB. orbicolaAU1054, indicating this species activates an alternative pathway of macrophage inflammatory death. Notably,B. cenocepaciaJ2315 and K56-2 can damage macrophage phagosomes and K56-2 triggers activation of the caspase-11 inflammasome, which detects cytosolic LPS. Here we investigated inflammatory cell death in pyrin-deficient (Mefv−/−) mouse macrophages infected withB. cenocepaciaJ2315 or K56-2 orB. orbicolaAU1054 or PC184. Macrophage inflammatory death was measured by cleavage of gasdermin D protein, release of cytokines IL-1α and IL-1β and plasma membrane rupture. Findings suggest that J2315 and K56-2 are detected by the caspase-11 inflammasome inMefv−/−macrophages, resulting in IL-1β release. In contrast, inflammasome activation is not detected inMefv−/−macrophages infected with AU1054 or PC184. Instead, AU1054 triggers an alternative macrophage inflammatory death pathway that requires TecA and results in plasma membrane rupture and IL-1α release. Amino acid variation between TecA isoforms inB. cenocepaciaandB. orbicolamay explain how the latter species triggers a non-inflammasome macrophage death pathway.
Human disturbance increases spatiotemporal associations among mountain forest terrestrial mammal species
Authors: Li Xueyou , Bleisch William V. , Hu Wenqiang , Li Quan , Wang Hongjiao , Chen Zhongzheng , Bai Ru , Jiang Xuelong 
Published: September 2023

AbstractSpatial and temporal associations between sympatric species underpin biotic interactions, structure ecological assemblages, and sustain ecosystem functioning and stability. However, the resilience of interspecific spatiotemporal associations to human activity remains poorly understood, particularly in mountain forests where anthropogenic impacts are often pervasive. Here we applied context-dependent Joint Species Distribution Models to a systematic camera-trap survey dataset from a global biodiversity hotspot in eastern Himalaya to understand how prominent human activities in mountain forests influence species associations within terrestrial mammal communities. We obtained 10,388 independent detections of 17 focal species (12 carnivores and five ungulates) from 322 stations over 43,163 camera days of effort. We identified a higher incidence of positive associations in habitats with higher levels of human modification (87 %) and human presence (83 %) compared to those located in habitats with lower human modification (64 %) and human presence (65 %) levels. We also detected a significant reduction of pairwise encounter time at increasing levels of human disturbance, corresponding to more frequent encounters between pairs of species. Our findings indicate that human activities can push mammals together into more frequent encounters and associations, which likely influences the coexistence and persistence of wildlife, with potential far-ranging ecological consequences.
Microinterfaces in bicontinuous hydrogels guide rapid 3D cell migration
Authors: Xu Karen L. , Caprio Nikolas Di , Fallahi Hooman , Dehghany Mohammad , Davidson Matthew D. , Cheung Brian C. H. , Laforest Lorielle , Wu Mingming , Shenoy Vivek , Han Lin , Mauck Robert L. , Burdick Jason A. 
Published: September 2023

AbstractCell migration is critical for tissue development and regeneration but requires extracellular environments that are conducive to motion. Cells may actively generate migratory routes in vivo by degrading or remodeling their environments or may instead utilize existing ECM microstructures or microtracks as innate pathways for migration. While hydrogels in general are valuable tools for probing the extracellular regulators of 3D migration, few have recapitulated these natural migration paths. Here, we developed a biopolymer-based (i.e., gelatin and hyaluronic acid) bicontinuous hydrogel system formed through controlled solution immiscibility whose continuous subdomains and high micro-interfacial surface area enabled rapid 3D migration, particularly when compared to homogeneous hydrogels. Migratory behavior was mesenchymal in nature and regulated by biochemical and biophysical signals from the hydrogel, which was shown across various cell types and physiologically relevant contexts (e.g., cell spheroids, ex vivo tissues, in vivo tissues). Our findings introduce a new design that leverages important local interfaces to guide rapid cell migration.
Central pore-opening structure and gating of the mechanosensitive PIEZO1 channel
Authors: Liu Sijia , Yang Xuzhong , Chen Xudong , Zhang Xiaochun , Jiang Jinghui , Wang Li , Yuan Jingyi , Liu Wenhao , Zhou Heng , Wu Kun , Tian Boxue , Li Xueming , Xiao Bailong 
Published: September 2023

AbstractPIEZO1 is a mechanically activated cation channel that undergoes force-induced activation and inactivation to control diverse physiological processes1–4. However, the distinct functional states and gating transitions remain structurally undefined. In response to changes in membrane forces, PIEZO1 transitions from a curved to a flattened shape, which might lead to opening and subsequent inactivation of the ion-conducting pore5,6. Here we employed a PIEZO1 mutant to capture various conformational states including an intermediate state with an opened central pore, allowing us to reveal the gating dynamics of this multiple-gate system. Upon transition from the curved to the partially flattened intermediate state, the trimeric cap undergoes a downward and rotational motion to open the lateral cap-gate, while the loop that connects the cap to the pore-lining inner helix acts as a spring-linker, switching from an extended to a compressed state. Along with the partial flattening of the peripheral blades, these conformational changes collectively dilate the cap-gate, spring-gate and the hydrophobic transmembrane-gate, leading to ion permeation through the transmembrane pore. Upon inactivation, while the blades remain flattened, the cap and spring-linker return to an up and extended state. Molecular dynamics simulations verify the ion-permeating pathway and the distinct ion-conducting states of the assigned close, intermediate, open, and inactivated structures. Mutagenesis and electrophysiological characterizations reveal that domains and residues involved in conformational dynamics of the cap and spring-linker are critical for mechanical activation of PIEZO1. Together, these studies have structurally defined the distinct functional states and the curvature-based activation and inactivation mechanisms of PIEZO1.
Lysine-36 ofDrosophilahistone H3.3 supports adult longevity
Authors: Brown John C. , McMichael Benjamin D. , Vandadi Vasudha , Mukherjee Aadit , Salzler Harmony R. , Matera A. Gregory 
Published: September 2023

AbstractAging is a multifactorial process that disturbs homeostasis, increases disease susceptibility, and ultimately results in death. Although the definitive set of molecular mechanisms responsible for aging remain to be discovered, epigenetic change over time is proving to be a promising piece of the puzzle. Several posttranslational histone modifications (PTMs) have been linked to the maintenance of longevity. Here, we focus on lysine-36 of the replication-independent histone protein, H3.3 (H3.3K36). To interrogate the role of this residue inDrosophiladevelopmental gene regulation, we generated a lysine to arginine mutant that blocks the activity of its cognate modifying enzymes. We found that anH3.3BK36Rmutation causes a significant reduction in adult lifespan, accompanied by dysregulation of the genomic and transcriptomic architecture. Transgenic co-expression of wild-typeH3.3Bcompletely rescues the longevity defect. Because H3.3 is known to accumulate in non-dividing tissues, we carried out transcriptome profiling of young vs aged adult fly heads. The data show that loss of H3.3K36 results in age-dependent misexpression of NF-κB and other innate immune target genes, as well as defects in silencing of heterochromatin. We propose H3.3K36 maintains the postmitotic epigenomic landscape, supporting longevity by regulating both pericentric and telomeric retrotransposons and by suppressing aberrant immune signaling.
SETD2 maintains nuclear lamina stability to safeguard the genome
Authors: Khan Abid , Metts James M. , Collins Lucas C. , Mills C. Allie , Li Kelin , Brademeyer Amanda L. , Bowman Brittany M. , Major M. Ben , Aubé Jeffrey , Herring Laura E. , Davis Ian J. , Strahl Brian D. 
Published: September 2023

AbstractHistone methyltransferases play essential roles in the organization and function of chromatin. They are also frequently mutated in human diseases including cancer1. One such often mutated methyltransferase, SETD2, associates co-transcriptionally with RNA polymerase II and catalyzes histone H3 lysine 36 trimethylation (H3K36me3) – a modification that contributes to gene transcription, splicing, and DNA repair2. While studies on SETD2 have largely focused on the consequences of its catalytic activity, the non-catalytic functions of SETD2 are largely unknown. Here we report a catalysis-independent function of SETD2 in maintaining nuclear lamina stability and genome integrity. We found that SETD2, via its intrinsically disordered N-terminus, associates with nuclear lamina proteins including lamin A/C, lamin B1, and emerin. Depletion of SETD2, or deletion of its N-terminus, resulted in widespread nuclear morphology abnormalities and genome stability defects that were reminiscent of a defective nuclear lamina. Mechanistically, the N-terminus of SETD2 facilitates the association of the mitotic kinase CDK1 with lamins, thereby promoting lamin phosphorylation and depolymerization required for nuclear envelope disassembly during mitosis. Taken together, our findings reveal an unanticipated link between the N-terminus of SETD2 and nuclear lamina organization that may underlie how SETD2 acts as a tumor suppressor.
Identifying individual factors associated with pleasure of eating healthy foods and plant/animal protein food consumption: findings from an exploratory study
Authors: Lambert-De Francesch Jessica , Labrecque JoAnne , Lessard Stéphanie 
Published: September 2023

PurposeThis study identifies new factors influencing the adoption of two recently promoted messages in Canada's updated food guide (FG): enhancing pleasure of eating healthy foods (PEHFs) and shifting food choices towards plant protein foods. Currently, limited and contradictory evidence is available regarding associations between environmental values, nutrition literacy, PEHFs, and plant/animal protein food consumption.Design/methodology/approachAn online survey measuring environmental values; nutrition literacy, distinctively based on previous (2007) and most recent (2019) FG messages; PEHFs; and annual changes in the consumption of protein foods was sent to Quebec residents (N = 128).FindingsGreater nutrition literacy of both 2007 and 2019 FGs and greater environmental values were associated with greater PEHFs (ß = 0.248, p < 0.01; ß = 0.209, p < 0.05; ß = 0.423, p < 0.001, respectively). Greater PEHFs was associated with greater consumption of plant protein foods (ß = 0.405, p < 0.001). Greater nutrition literacy of the 2007 FG was associated with greater consumption of animal protein foods (ß = 0.409, p < 0.001), whereas greater nutrition literacy of the 2019 FG was linked to lower consumption of animal protein foods (ß = −0.225, p < 0.05).Practical implicationsEnhancing PEHFs may require increasing general FG nutrition literacy and strengthening environmental values. To encourage plant protein food consumption and decrease animal protein food consumption, the authors recommend promoting PEHFs and increasing nutrition literacy based on newest FG recommendations.Originality/valueThis new evidence may help develop strategies promoting PEHFs and plant protein food consumption, thus increasing uptake of new FG recommendations.
A novel strategy segmentation methodology integrating Kraljic portfolio matrix and supplier relationship model: a case study from machinery industry
Authors: Yalcin Ahmet Selcuk , Kilic Huseyin Selcuk , Cevikcan Emre 
Published: September 2023

PurposeThe purpose of this article is to develop a new model called strategy segmentation methodology (SSM) by combining the Kraljic portfolio matrix (KPM) and the supplier relationship model (SRM) so that the buyer company can effectively conduct its relations with its suppliers.Design/methodology/approachThe importance weights of the criteria defining the dimensions of each model are calculated with the single-valued neutrosophic analytical hierarchy process (SVN-AHP) method. Subsequently, the derived importance weights are employed in the single-valued neutrosophic technique for order preference by similarity to ideal solution (SVN-TOPSIS) method to obtain the scores of the suppliers and their supplied items. In order to illustrate the feasibility of the proposed methodology, a case study in the machinery industry is performed with the related comparative analysis.FindingsThe implementation of SSM enables to formulate various strategies to manage suppliers taking into account the items they procure, their capabilities and performance and the supplier–buyer relationship strength. Based on the proposed strategies, it is concluded that the firm in the case study should terminate its relationship with six of its suppliers.Originality/valueAlthough KPM has become the basis of purchasing strategies for various businesses, it neglects the characteristics of suppliers and the buyer–supplier relationship. In this study, KPM is integrated with the SRM approach presented by Olsen and Ellram (1997) to overcome these disadvantages of KPM. The novel integration of the two approaches enables the realization of a robust and reliable supplier classification model.
Local/volumetric entropy production analysis of natural convective flow using lattice Boltzmann method for heat exchanger application
Authors: Hasani Malekshah Emad , Kolsi Lioua 
Published: September 2023

Purpose
The purpose of this study is the hydrothermal analysis of the natural convection phenomenon within the heat exchanger containing nanofluids using the lattice Boltzmann method (LBM).

Design/methodology/approach
The thermal conductivity as well as dynamic viscosity of the CuO–water nanofluid is estimated using the Koo-Kleinstreuer-Li model. The LBM has been used with unique modifications to make it flexible with the curved boundaries. The local as well as total entropy generation assessment, local Nusselt variation, as well as heatline visualization are used.

Findings
The solid volume percentage of the CuO–water nanofluid, a range of Rayleigh numbers (Ra) and thermal settings of internal operational fins and bodies are all factors that have been thoroughly researched to determine their effects on entropy production, heat transfer efficiency and nanofluid flow.

Originality/value
The originality of this work is using a novel numerical method (i.e. curved boundary LBM) as well as the local/volumetric second law analysis for the application of heat exchanger hydrothermal analysis.
Equity capital financing of Swedish SMEs, innovation, and decentralized management
Authors: Ström Viktor , Sanandaji Nima , Esmaeilzadeh Saeid , Esmaeilzadeh Mouna 
Published: September 2023

Purpose
The purpose of this paper is to investigate the potential link between Sweden’s high reliance on equity capital financing among small and medium-sized enterprises (SMEs) and its recognition as the most innovative economy in Europe according to the European Innovation Scoreboard (EIS). This paper examines the idea that the high levels of trust within Swedish society can explain why private equity financing is more prevalent among Swedish SMEs.

Design/methodology/approach
To test these ideas, the authors use data from the Survey on Access to Finance for Enterprises to measure the private equity reliance of firms. The authors also use the EIS to measure the innovation capacity of nations and various aspects of SMEs’ innovation activities. Finally, societal levels of trust are measured through the World Value Survey.

Findings
First, the authors find that European countries with a higher proportion of SMEs relying on equity financing tend to be ranked as more innovative by the EIS. Second, the authors find that the correlation between a nation’s share of SMEs relying on equity financing and their level of innovation activities is marginally stronger for product innovations than for business process innovations. Third, the authors find that countries with higher levels of trust tend to have higher equity capital reliance among SMEs.

Originality/value
This study builds upon previous research on equity capital and SMEs’ innovation activity while introducing new insights into the relationship between societal trust and equity financing.
Dimensions and outcomes of electronic service quality in the GCC countries
Authors: Thanasi-Boçe Marsela , Kulakli Atik 
Published: September 2023

Purpose
The rapid growth of e-commerce and the increasing importance of online services have prompted the need for managers to understand what consumers value most in online transactions and how to measure electronic service quality (E-SQ). This study aims to conduct a comprehensive review of relevant research publications in the Gulf Cooperation Council countries and propose an agenda for future research to enhance this knowledge.

Design/methodology/approach
The study uses a blended approach between the PRISMA-ScR methodology and Tranfield et al.’s (2003) approach to ensure a rigorous and thorough review of the literature.

Findings
By identifying the antecedents and outcomes of E-SQ, this study develops a classification framework that highlights the main gaps in the current literature and provides guidance for future research in the region.

Practical implications
The findings presented in this paper contribute to the knowledge base for management professionals seeking to enhance E-SQ in practice.

Originality/value
To the best of the authors’ knowledge, this is the first paper to summarize the state of E-SQ in the Gulf region.
Developing capabilities underlying to Industry 4.0 design principles within the manufacturing context
Authors: Ghobakhloo Morteza , Vilkas Mantas , Stefanini Alessandro , Grybauskas Andrius , Marcinkevicius Gediminas , Petraite Monika , Sarvari Peiman Alipour 
Published: September 2023

PurposeUsing a dynamic capabilities approach, the present study aims to identify and assess the effects of organizational determinants on capabilities underlying Industry 4.0 design principles, such as integration, virtualization, real-time, automation and servitization.Design/methodology/approachPLS-SEM enables a two-stage hierarchical latent variable reflective-formative model which was used for assessing the effect of organizational determinants on Industry 4.0 design principles. Five hundred six manufacturing companies constitute the effective sample, representing a population of manufacturing companies in an industrialized country.FindingsThe findings reveal that Industry 4.0 design principles extensively depend on digitalization resource availability. At the same time, companies that possess digitalization and change management capabilities tend to devote more resources to digitalization. Finally, the paper reveals that networking and partnership capability is the critical enabler for change management and digitalization capabilities.Practical implicationsThe paper provides empirical evidence that the successful development of Industry 4.0 design principles and their underlying integration, virtualization, real-time, automation and servitization capabilities are resource dependent, requiring significant upfront investment and continuous resource allocation. Further, the study implies that companies with networking and partnership, change management and digitalization capabilities tend to allocate more resources for Industry 4.0 transformation.Originality/valueExclusively focusing on empirical research that reported applied insights into determinants of Industry 4.0 design principles, the study offers unique implications for promoting Industry 4.0 digital transformation among manufacturing companies.
Information literacy and the material objects of the Kente-weaving landscape
Authors: Agyemang Franklin Gyamfi , Wessels Nicoline , du Preez Madely 
Published: September 2023

Purpose
This paper aims to examine the ways becoming information literate relates to the material objects in the Kente-weaving landscape.

Design/methodology/approach
An ethnographic research design was adopted wherein data was collected using participant observation and a semi-structured interview with 24 participants through their roles as either master weaver, junior weaver or novice weaver. Thematic analysis through a practice-based approach to information literacy frames the analysis of this study.

Findings
Information literacy relates to the material objects in terms of developing the know-how knowledge regarding the Kente-weaving tools used as well as what constitutes the quality of Kente fabrics.

Practical implications
Information literacy goes beyond having theoretical knowledge of the material objects of an information landscape. It is practical, not merely knowing the names of the material objects and what they are literary used for.

Originality/value
To the best of the authors’ knowledge, this is the first study that contributes to the understanding of how information literacy relates to material objects in the craft workplace.
The optimal decision of e-retailer based on return-freight insurance – considering the loss aversion of customers
Authors: Zhan Wentao , Pan Wenting , Zhao Yi , Zhang Shengyu , Wang Yimeng , Jiang Minghui 
Published: September 2023

PurposeThe return behavior of customers has a great impact on the e-retail industry and has resulted in the emergence of return-freight insurance (RI). Additionally, customer loss aversion arising from returns affects e-retailers' decisions and manufacturers' profits. Therefore, the main purpose of the authors' study is to determine how e-retailers and manufacturers choose their RI strategy and pricing according to customers' loss aversion.Design/methodology/approachThe authors propose three scenarios: no RI, customer purchase RI and free e-retail RI (FRI). Meanwhile, the authors also model a Stackelberg game between e-retailers and manufacturers for analysis. Then, according to customer return behavior and loss aversion, the authors study the optimal pricing decision and RI premium allocation scheme for e-retailers and manufacturers under different scenarios.FindingsIt was found that the loss sensitivity reduces customers' willingness to buy RI, which is not conducive to the development of e-retailers and manufacturers. Additionally, with higher loss sensitivity, e-retailers and manufacturers offer FRI to gain higher profits, which supports the implementation of the FRI strategy.Originality/valueThe authors introduce customers' loss aversion into RI to analyze the optimal pricing decisions and profits of e-retailers and manufacturers, enriching the application of loss aversion theory. In addition, this study analyzes the two-way cost-sharing mechanism between manufacturers and e-retailers to provide FRI, which provides a theoretical basis for RI premium sharing.