bluuebunny/crossref_metadata_2025_split huggingface dataset:
The many roles of cyclic di-AMP to control the physiology of Bacillus subtilis
Authors: Herzberg Christina , Meißner Janek , Warneke Robert , Stülke Jörg 
Published: October 2023

Abstract
The dinucleotide cyclic di-AMP (c-di-AMP) is synthesized as a second messenger in the Gram-positive model bacterium Bacillus subtilis as well as in many bacteria and archaea. Bacillus subtilis possesses three diadenylate cyclases and two phosphodiesterases that synthesize and degrade the molecule, respectively. Among the second messengers, c-di-AMP is unique since it is essential for B. subtilis on the one hand but toxic upon accumulation on the other. This role as an “essential poison” is related to the function of c-di-AMP in the control of potassium homeostasis. C-di-AMP inhibits the expression and activity of potassium uptake systems by binding to riboswitches and transporters and activates the activity of potassium exporters. In this way, c-di-AMP allows the adjustment of uptake and export systems to achieve a balanced intracellular potassium concentration. C-di-AMP also binds to two dedicated signal transduction proteins, DarA and DarB. Both proteins seem to interact with other proteins in their apo state, i.e. in the absence of c-di-AMP. For DarB, the (p)ppGpp synthetase/hydrolase Rel and the pyruvate carboxylase PycA have been identified as targets. The interactions trigger the synthesis of the alarmone (p)ppGpp and of the acceptor molecule for the citric acid cycle, oxaloacetate, respectively. In the absence of c-di-AMP, many amino acids inhibit the growth of B. subtilis. This feature can be used to identify novel players in amino acid homeostasis. In this review, we discuss the different functions of c-di-AMP and their physiological relevance.
Diversification of small RNA pathways underlies germline RNA interference incompetence in wild Caenorhabditis elegans strains
Authors: Chou Han Ting , Valencia Francisco , Alexander Jacqueline C , Bell Avery Davis , Deb Diptodip , Pollard Daniel A , Paaby Annalise B 
Published: October 2023

Abstract
The discovery that experimental delivery of dsRNA can induce gene silencing at target genes revolutionized genetics research, by both uncovering essential biological processes and creating new tools for developmental geneticists. However, the efficacy of exogenous RNA interference (RNAi) varies dramatically within the Caenorhabditis elegans natural population, raising questions about our understanding of RNAi in the lab relative to its activity and significance in nature. Here, we investigate why some wild strains fail to mount a robust RNAi response to germline targets. We observe diversity in mechanism: in some strains, the response is stochastic, either on or off among individuals, while in others, the response is consistent but delayed. Increased activity of the Argonaute PPW-1, which is required for germline RNAi in the laboratory strain N2, rescues the response in some strains but dampens it further in others. Among wild strains, genes known to mediate RNAi exhibited very high expression variation relative to other genes in the genome as well as allelic divergence and strain-specific instances of pseudogenization at the sequence level. Our results demonstrate functional diversification in the small RNA pathways in C. elegans and suggest that RNAi processes are evolving rapidly and dynamically in nature.
Numerical thermomechanical modelling of lava dome growth during the 2007–2009 dome-building eruption at Volcán de Colima
Authors: Zeinalova Natalya , Ismail-Zadeh Alik , Tsepelev Igor , Melnik Oleg , Schilling Frank 
Published: October 2023

SUMMARY
Lava domes form during effusive eruptions due to an extrusion of highly viscous magmas from volcanic vents. In this paper we present a numerical study of the lava dome growth at Volcán de Colima, Mexico during 2007–2009. The mathematical model treats the lava dome extrusion dynamics as a thermomechanical problem. The equations of motion, continuity and heat transfer are solved with the relevant boundary and initial conditions in the assumption that magma viscosity depends on the volume fraction of crystals and temperature. We perform several sets of numerical experiments to analyse the internal structure of the lava dome (i.e. the distributions of the temperature, crystal content, viscosity and velocity) depending on various heat sources and thermal boundary conditions. Although the lava dome growth at Volcán de Colima during short (a few months) dome-building episodes can be explained by an isothermal model of lava extrusion with the viscosity depending on the volume fraction of crystals, we show here that cooling plays a significant role during long (up to several years) episodes of dome building. A carapace develops as a response to a convective cooling at the lava dome–air interface. The carapace becomes thicker if the radiative heat loss at the interface is also considered. The thick carapace influences the lava dome dynamics preventing its lateral advancement. The release of the latent heat of crystallization leads to an increase of the temperatures in the lava dome interior and to a relative flattening of the dome. Meanwhile, the heat source due to viscous dissipation inside the lava dome is negligible, and it does not influence the lava dome growth. The developed thermomechanical model of the lava dome dynamics at Volcán de Colima can be used elsewhere to analyse effusive eruptions, dome morphology and carapace evolution including its failure potentially leading to pyroclastic flow hazards.
Pivot: partisan policy responses to COVID-19 health disparities
Authors: Creary Melissa S , Greer Scott L , Kitzmiller Sydney , Singer Phillip M , Unwala Naquia A , Vaziri Soha , Willison Charley E 
Published: October 2023

Abstract
How did partisanship influence rhetoric about, public opinion of, and policies that prioritize racial and ethnic health disparities of COVID-19 during the first wave of the pandemic between March and July 2020? In this retrospective, mixed-methods analysis using national administrative and survey data, we found that the rhetoric and policy of shared sacrifice diminished and partisan differences in pandemic policy increased once it became clear to political elites that there were major racial disparities in COVID-19 cases and deaths. We trace how first disparities emerged in data and then were reported in elite, national media, discussed in Congress, and reflected in public opinion. Once racial disparities were apparent, partisan divides opened in media, public opinion, and legislative activity, with Democrats foregrounding inequality and Republicans increasingly downplaying the pandemic. This temporal dimension, focusing on how the diffusion of awareness of inequalities among elites shaped policy in the crucial months of early 2020, is the principal novel finding of our analysis. Overall, there is a clear, partisan policy response to addressing COVID-19 racial disparities across media, public opinion, subnational legislative activity, and congressional deliberations.
Lobbying by omission: what is known and unknown about harmful industry lobbyists in Australia
Authors: Lacy-Nichols Jennifer , Christie Shirae , Cullerton Katherine 
Published: October 2023

Abstract
What is unknown about commercial lobbying is far greater than what is known. These omissions distort our understanding of the extent and nature of business influence on politics. Especially when businesses engage in practices that harm health, it is crucial for public health advocates to understand corporate lobbying to counter its influence. Our study proceeded in three phases. First, based on an international audit, we developed a list of the categories of information about lobbying that could be disclosed under four groups (lobby firms, lobbyists, organizations and activities) and benchmarked Australian lobbyist registers against this list. Second, we manually extracted data from lobbyist registers in eight jurisdictions, cleaned the data and created a relational model for analysis. Finally, we classified a sample of organizations as public health organizations or harmful industries to compare their activities. We identified 61 possible categories of information about lobbying in international lobbyist registers. When applied to Australian lobbyist registers, Queensland covered the widest range of categories (13, 21%), though many lacked detail and completeness. Australian lobbyist registers provided data on 462 third-party lobby firms across Australia, currently employing 1036 lobbyists and representing 4101 organizations. Several of these represented harmful industries, with gambling interests hiring the most third-party lobby firms. Ultimately, Australian lobbyist registers do not provide enough information to understand the full extent of lobbying activities taking place. Political transparency is important for public health actors to be able to monitor corporate political activity and to protect policy-making from vested interests.
The Impact of Heroin Self-Administration and Environmental Enrichment on Ventral Tegmental CRF1 Receptor Expression
Authors: Galaj Ewa , Barrera Eddy D , Persaud Kirk , Nisanov Rudolf , Vashisht Apoorva , Goldberg Hindy , Patel Nima , Lenhard Hayley , You Zhi-Bing , Gardner Eliot L , Ranaldi Robert 
Published: October 2023

Abstract

Background
There is a strong link between chronic stress and vulnerability to drug abuse and addiction. Corticotropin releasing factor (CRF) is central to the stress response that contributes to continuation and relapse to heroin abuse. Chronic heroin exposure can exacerbate CRF production, leading to dysregulation of the midbrain CRF-dopamine-glutamate interaction.

Methods
Here we investigated the role of midbrain CRF1 receptors in heroin self-administration and assessed neuroplasticity in CRF1 receptor expression in key opioid addiction brain regions.

Results
Infusions of antalarmin (a CRF1 receptor antagonist) into the ventral tegmental area (VTA) dose dependently reduced heroin self-administration in rats but had no impact on food reinforcement or locomotor activity in rats. Using RNAscope in situ hybridization, we found that heroin, but not saline, self-administration upregulated CRF1 receptor mRNA in the VTA, particularly on dopamine neurons. AMPA GluR1 and dopamine reuptake transporter mRNA in VTA neurons were not affected by heroin. The western-blot assay showed that CRF1 receptors were upregulated in the VTA and nucleus accumbens. No significant changes in CRF1 protein expression were detected in the prefrontal cortex, insula, dorsal hippocampus, and substantia nigra. In addition, we found that 15 days of environmental enrichment implemented after heroin self-administration does not reverse upregulation of VTA CRF1 receptor mRNA but it downregulates dopamine transporter mRNA.

Conclusions
Overall, these data suggest that heroin self-administration requires stimulation of VTA CRF1 receptors and upregulates their expression in brain regions involved in reinforcement. Such long-lasting neuroadaptations may contribute to continuation of drug use and relapse due to stress exposure and are not easily reversed by EE exposure.
Augmented surveillance of antimicrobial resistance with high-throughput robotics detects transnational flow of fluoroquinolone-resistant Escherichia coli strain into poultry
Authors: Truswell Alec , Lee Zheng Zhou , Stegger Marc , Blinco John , Abraham Rebecca , Jordan David , Milotic Marin , Hewson Kylie , Pang Stanley , Abraham Sam 
Published: October 2023

Abstract

Background
Food animal AMR surveillance programs assess only small numbers of Escherichia coli (from 100 to 600 per animal class) nationally each year, severely limiting the evaluation of public health risk(s). Here we demonstrate an affordable approach for early detection of emerging resistance on a broad scale that can also accurately characterize spatial and temporal changes in resistance.

Methods
Caecal samples (n = 295) obtained from 10 meat poultry were screened using high-throughput robotics. Initial screening via agar dilution (5310 plates) quantified AMR carriage (cfu/g) for each sample. Ciprofloxacin-resistant isolates (n = 91) proceeded to downstream broth microdilution susceptibility testing. A subset of 28 ciprofloxacin-resistant isolates underwent WGS and phylogenetic analysis.

Results
Intra- and inter-flock carriage of resistance varied with drug class. Ampicillin and tetracycline resistance was ubiquitous to most birds in all flocks with an average carriage rate of 5.8 log10 cfu/g. Gentamicin and ciprofloxacin-resistant E. coli colonized fewer birds, and had an average carriage rate of 1.2 log10 cfu/g and 1.0 log10 cfu/g of faeces, respectively. Resistance to extended-spectrum cephalosporins was absent. ST354 was the dominant ST among the WGS isolates, but they demonstrated markedly lower resistance gene carriage than their international counterparts.

Conclusions
These data amply demonstrate the ineffectiveness of commonly relied-on approaches to AMR surveillance for achieving early detection of emergence, or for measuring spatial and temporal resistance trends. Genetic analysis suggested there has been transnational flow of a ciprofloxacin-resistant strain into Australian poultry flocks, explaining their detection in a nation that prohibits fluoroquinolone use in poultry.
The value affordances of social media engagement features
Authors: Scharlach Rebecca , Hallinan Blake 
Published: October 2023

AbstractSocial media engagement is ubiquitous but contested, simultaneously framed as an everyday form of support and an urgent societal risk. To make sense of these competing claims, we introduce the concept of value affordances, defined as the set of ethical, aesthetic, and relational principles that emerge from the interaction between different stakeholders and technological infrastructures. We develop a novel method involving focus groups and value cards to study the value affordances of engagement features and explore how international students attribute values to the Like, Comment, and Share buttons of TikTok, Instagram, and YouTube. Across platforms, participants agree that engagement features promote expression, care, and community and hinder privacy, mindfulness, peace, and safety. We discuss how our participants navigate value tradeoffs, emphasizing individual agency over structural factors when evaluating the design of platforms, using social media creatively, and assigning responsibility for harm to other users.
From gender segregation to epistemic segregation: a case study of the school system in Iran
Authors: Heidarifar Shadi 
Published: October 2023

Abstract
In this paper, I show that there is a bidirectional relationship between gender-based social norms and gender-segregated education policies that excludes girls from knowledge production within the Iranian school system. I argue that gender segregation in education reproduces hermeneutic inequality through the reinforcement of epistemic segregation as a form of epistemic injustice. In particular, I focus on gender-based instructional epistemic injustice, which refers to a set of epistemic practices that actively exclude a student or an education professional in their capacity as a knower from the process of knowledge production within an education system based on gender dynamics, roles, norms, or expectations. This, in addition, has an impact on schoolboys through the reproduction of active ignorance. I conclude that in societies such as Iran, where highly gendered norms play out in the school system and are further reinforced by that system, the result is not limited to gender segregation itself, but extends beyond it to a form of epistemic injustice that wrongs students by reproducing and reinforcing those highly gendered norms.
Establishment of real-time fluorescence and visual LAMP for rapid detection of Escherichia coli O157:H7 and kits construction
Authors: Wang Zuwei , Cui Xinping , Hu Antuo , Lu Zhaoxin , Meng Fanqiang , Zhou Libang , Bie Xiaomei 
Published: October 2023

Abstract
Escherichia coli O157:H7 is a common pathogenic bacterium in food and water that can pose a threat to human health. The aim of this study was to develop loop-mediated isothermal amplification (LAMP) method for the detection of E. coli O157:H7 in food based on the specific gene Ecs_2840 and to construct rapid detection kits based on the established methods. Specifically, we established two methods of real-time fluorescent LAMP (RT-LAMP) and visual LAMP with calcein as an indicator. In pure bacterial culture, the cell sensitivity and genomic sensitivity of the RT-LAMP kit were 8.8 × 100 CFU ml−1 and 4.61 fg µl−1, respectively. The sensitivity of the visual LAMP kit was 2.35 × 100 CFU ml−1 and 4.61 fg µl−1. Both kits had excellent specificity and anti-interference performance. In addition, milk inoculated with 2.26 × 100 CFU ml−1E. coli O157:H7 could be detected within the reaction time after enrichment for 3 h. The results showed that the LAMP kits were rapid, sensitive, and specific for the detection of E. coli O157:H7 in food and had good application prospects in food safety surveillance.
Anterior Cruciate Ligament Reconstruction in French Army: Return to Prior Level of Running on Selected Military Tests
Authors: de Geofroy Bernard , Trescos Florent , Ghabi Ammar , Choufani Camille , Peras Mathieu , Barbier Olivier , de Landevoisin Emmanuel , Jouvion Arnaud-Xavier 
Published: October 2023

ABSTRACT

Introduction
Anterior cruciate ligament (ACL) rupture is frequently encountered in athletes as well as in military personnel. In civilian population, many studies have looked at the return to sport, but return to duty in Army is a topic that requires further research.
The purpose of this study was to determine through annual military fitness tests in real conditions, the return to sport in soldiers after ACL reconstruction and factors influencing failure.

Materials and Methods
This was a retrospective comparative study. Patients were all soldiers and had followed up in a Military Hospital. The SUCCESS group was military personnel who obtained a result of the specific aptitude test greater than or equal to this same test carried out before reconstruction of the ACL, the FAILURE group comprised the others. Results of the annual specific aerobic fitness tests were collected before and after ACL reconstruction. Preoperative epidemiological data, intraoperative information, and isokinetic test results were collected.

Results
One hundred forty four soldiers were included between January 2011 and December 2017 (94.9% of men with a median age of 27.6 years); 40.3% obtained a result greater than or equal to the preoperative fitness test after ACL reconstruction. Among the soldiers who did not regain their performance, 24.3% were declared unfit or discharged. In the FAILURE group, we found patients with a higher body mass index (25.5 vs. 24.4; P = .04), less patients with isokinetic deficit &lt; 30% on the knee flexors and extensors (26.6% vs. 62.9%; P &lt; .01), more long sick leave (39.5% vs. 13.7%; P &lt; .01), and late resumption of military activities (10.5 vs. 8.9 months; P &lt; .01).

Conclusion
Rupture of ACL has a significant impact on the operational capacity of the French army. The proportion of return to the same level in annual specific fitness tests after ACL reconstruction is 40% among soldiers. Several variables are important to consider in the follow-up of these patients to optimize their recovery of sports performance and therefore their operational capacity.
Support for Complicated Grief in Military Primary Care: Associations, Challenges, and Opportunities
Authors: Gibson Lauren P 
Published: October 2023

ABSTRACT

Objective
This study examined whether service members and their families engage in communication with their primary care managers (PCMs) regarding their losses and to what extent PCMs provide counseling or referrals for grief-related concerns. Additionally, the study explored the potential associations between ethnicity, age, military status (dependent, service member, or veteran), gender, patients’ disclosure of loss to their PCM, receipt of referrals for grief-related services, and treatment seeking.

Methods
A survey was administered to 161 patients enrolled in primary care at William Beaumont Army Medical Center, with 138 reporting a significant loss. Bereaved respondents completed the Inventory of Complicated Grief and the Prolonged Grief Disorder-13 Revised scale (PG-13). Respondents were also asked questions related to their loss and their interactions with their PCM related to the loss.

Results
One hundred twenty-five bereaved respondents completed the measures, 13 had missing information related to PCM interactions. Twenty-nine percent (n = 36) of the 112 respondents reported a significant loss to their PCM. These respondents exhibited significantly higher symptom severity, as indicated by their total mean scores on the Inventory of Complicated Grief and the PG13. Seventeen percent (n = 6) of these respondents reported receiving counseling on topics related to grief or a referral for grief care from their PCM. A moderate relationship was identified between military status and communication with PCM, such that current service members were least likely to report, followed by veterans, then dependents. A moderate relationship was also found between ethnicity and treatment seeking.

Conclusion
The study’s findings have implications for primary care and highlight opportunities for improved outcomes, including the development of appropriate assessment protocols, interventions, and educational initiatives to address grief-related concerns within the military healthcare system. Future research should further investigate the specific needs and preferences of military personnel and their families to enhance the delivery of targeted and effective healthcare services.