bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcranial Photobiomodulation on the Left Prefrontal Cortex Enhances Mandarin Chinese L1 and L2 Complex Sentence Processing Performances
Authors: Yang Mingchuan , Liu Yang , Yue Zhaoqian , Yang Guang , Jiang Xu , Cai Yimin , Zhang Yuqi , Yang Xiujie , Li Dongwei , Chen Luyao 
Published: January 2024

AbstractThis study investigated the causal effect of transcranial photobiomodulation (tPBM) over the left prefrontal cortex (LPFC) on syntactically complex Mandarin Chinese first language (L1) and second language (L2) sentence processing performances. Two (L1 and L2) groups of participants (thirty per group) were recruited to receive the double-blind, sham-controlled tPBM intervention, followed by the sentence processing, the verbal working memory (WM), and the visual WM tasks. Results revealed a consistent pattern for both groups: (a) tPBM enhanced sentence processing performance but not verbal WM and visual WM performance; (b) Participants with lower sentence processing performances under sham tPBM benefited more from active tPBM. Taken together, the current study substantiated that tPBM enhanced L1 and L2 sentence processing ability directly without verbal WM interference, and would serve as a promising and cost-effective noninvasive brain stimulation (NIBS) tool for future applications on upregulating the human language faculty.HighlightsThe first study that applies tPBM to (complex) sentence processing.tPBM enhances sentence processing performances in both Mandarin L1 & L2 speakers.tPBM directly enhances sentence processing without the interference of verbal WM.A causal role of LPFC for sentence processing through active tPBM.Opening up the promising application prospect for tPBM on sentence processing.
Identification and classification of the genomes of novel Microviruses in poultry slaughterhouse
Authors: Xie Ke-Ming , Lin Ben-Fu , Zhu Peng , Sun Xin-Yu , Liu Chang , Liu Guang-Feng , Cao Xu-Dong , Pan Jing-Qi , Qiu Sui-Ping , Yuan Xiao-Qi , Liang Meng-Shi , Jiang Jing-Zhe , Yuan Li-Hong 
Published: January 2024

AbstractMicroviridae is a family of phages with circular ssDNA genomes and they are widely found in various environments and organisms. In this study, Virome techniques were employed to explore potential members of Microviridae in poultry slaughterhouse, leading to the identification of 98 novel and complete microvirus genomes. Using a similarity clustering network classification approach, these viruses were found to belong to at least 6 new subfamilies within Microviridae and 3 higher-level taxonomic units. Analysis of their genomes found that the genome size, GC content and genome structure of these new taxa showed evident regularities, validating the rationality of our classification method. Compared with the 19 families classified by previous researchers for microviruses dataset, our method can divide microviruses into about 45 more detailed clusters, which may serve as a new standard for classifying Microviridae members. Furthermore, addressing the scarcity of host information for microviruses, this study significantly broadened their host range and discovered over 20 possible new hosts, including important pathogenic bacteria such as Helicobacter pylori and Vibrio cholerae, as well as different taxa demonstrated differential host specificity. The findings of this study effectively expand the diversity of the Microviridae, providing new insights for their classification and identification. Additionally, it offers a novel perspective for monitoring and controlling pathogenic microorganisms in poultry slaughterhouse environments.
Standardised Measurements for Monitoring and Comparing Multiphoton Microscope Systems
Authors: Lees Robert M. , Bianco Isaac H. , Campbell Robert A.A. , Orlova Natalia , Peterka Darcy S. , Pichler Bruno , Smith Spencer LaVere , Yatsenko Dimitri , Yu Che-Hang , Packer Adam M. 
Published: January 2024

AbstractThe goal of this protocol is to enable better characterisation of multiphoton microscopy hardware across a large user base. The scope of this protocol is purposefully limited to focus on hardware, touching on software and data analysis routines only where relevant. The intended audiences are scientists using and building multiphoton microscopes in their laboratories. The goal is that any scientist, not only those with optical expertise, can test whether their multiphoton microscope is performing well and producing consistent data over the lifetime of their system.
DEFICIENCY OF THE NUTRIENT SENSOR CPT1c IN SF1 NEURONS DISRUPTS THE ENDOCANNABINOID SYSTEM RESULTING IN COMPROMISED SATIETY AND FUEL SELECTION UPON FAT INTAKE
Authors: Fosch A , Pizarro DS , Zagmutt S , Reguera AC , Batallé G , Rodríguez-García M , García-Chica J , Freire-Agulleiro O , Miralpeix C , Zizzari P , Serra D , Herrero L , López M , Cota D , Rodríguez-Rodríguez R , Casals N 
Published: January 2024

ABSTRACTThe SF1 neurons of the ventromedial hypothalamus (VMH) are pivotal in governing body weight and adiposity, particularly in response to a high-fat diet (HFD). Previous studies have shown that the activation of SF1 neurons induces satiety, increases energy expenditure, and promotes the preferential use of fats as energy substrate. Furthermore, SF1 neurons are necessary for recovering from insulin-induced hypoglycemia. Here we demonstrate the essential role of the nutritional sensor CPT1c in the activation of SF1 neurons by dietary fats. Mice deficient in CPT1C in SF1 neurons (SF1-CPT1c-KO) are unable to adjust their caloric intake during the initial exposure to a HFD. This is associated with an impaired metabolic transition in the liver, muscle, and adipose tissue, despite a normal response to a glucose or insulin challenge. During chronic HFD exposure, SF1-CPT1c-KO mice are more prone to obesity and glucose intolerance than controls. CPT1c deficiency in SF1 neurons also leads to alterations in hypothalamic endocannabinoid levels and their metabolism. Our findings posit CPT1C in SF1 neurons as a sensor for dietary fats, regulating satiety responses and nutrient partitioning likely through the modulation of the endocannabinoid system.
Exploring social modulation: Microglia as a key mediator of individual immune response, plasticity and pathology in App-NL-G-F mouse model of Alzheimer’s disease
Authors: Ehret Fanny , Doludda Birte , Liu Hang , Nexhipi Sindi , Huang Hao , Barde Warsha , Rost Fabian , Overall Rupert , Dahl Andreas , Schmidt Mirko HH , Sieweke Michael , Kempermann Gerd 
Published: January 2024

ABSTRACTThis study explores the influence of lifestyle on Alzheimer’s disease (AD) progression using App-NL-G-F mice in a complex enrichment system. Mice exhibited social deficits before plaque pathology or memory impairment, revealing a crucial link between lifestyle, behavior, and neuroinflammation. Plasma analysis indicates early inflammation and apoptosis-related changes, setting the stage for identifying markers predicting plaque manifestation. Beyond pathology, social behavior is linked to adult neurogenesis and microglia coverage, forming a dynamic connection with microglia activation. Further, sc-RNA sequencing unveiled a decrease in interferon-responsive microglia and alteration in antigen processing with enrichment. These findings underscore the beneficial impact of social housing on microglia and interconnected factors, pointing to microglia as a critical mediator of the behavior-pathology-plasticity interplay in AD. The study enhances our understanding of AD complexity and offers insights into potential therapeutic strategies, emphasizing the multifaceted nature of AD progression and the role of lifestyle in shaping its course.
Biphasic Changes in Hippocampal Granule Cells after Traumatic Brain Injury
Authors: Danielewicz Joanna , Llamosas Nerea , Durá Irene , Barros de Souza Danillo , Rodrigues Serafim , Encinas-Pérez Juan Manuel , Mateos Diego Martin 
Published: January 2024

AbstractTraumatic brain injury (TBI) leads to a wide range of long-lasting physical and cognitive impairments. Changes in neuronal excitability and synaptic functions in the hippocampus have been proposed to underlie cognitive alterations. The dentate gyrus (DG) acts as a “gatekeeper” of hippocampal information processing and as a filter of excessive or aberrant input activity. In this study, we investigated the effects of controlled cortical impact, a model of TBI, on the excitability of granule cells (GCs) and excitatory postsynaptic transmission in the DG at three time points, 3 days, 15 days and 4 months after the injury. Our results indicate that changes in the short term are related to intrinsic properties, while changes in the long term are more related to input and synaptic activity, in agreement with the notion that TBI-related pathology courses with an acute phase and a later long-term secondary phase. A biphasic response, a reduction in the shorter term and an increase in the long term, was found in TBI neurons in the frequency of sEPSC. These changes correlated with a loss of complexity in the pattern of the synaptic input, an alteration that could therefore play a role in the chronic and recurrent TBI-asssociated hyperexcitation.
Posture-dependent modulation of marmoset cortical motor maps detected via rapid multichannel epidural stimulation
Authors: Takemi Mitsuaki , Tia Banty , Kosugi Akito , Castagnola Elisa , Ansaldo Alberto , Ricci Davide , Fadiga Luciano , Ushiba Junichi , Iriki Atsushi 
Published: January 2024

AbstractIn this study, rapid topographical changes were detected in the forelimb motor maps in the primary motor cortex (M1) of awake marmoset monkeys using our previously developed accurate short-time stimulation mapping procedure (Takemi et al. 2017; Kosugi et al. 2018). The results revealed that although the hotspot (the location in M1 that elicited a forelimb muscle twitch with the lowest stimulus intensity) remained constant across postures, the stimulus intensity required to elicit the forelimb muscle twitch in the perihotspot region and the size of motor representations were posture-dependent. Hindlimb posture was particularly effective in inducing these modulations. The angle of the body axis relative to the gravitational vertical line did not alter the motor maps. These results provide a proof of concept that a rapid stimulation mapping system with chronically implanted cortical electrodes can capture the dynamic regulation of forelimb motor maps in natural conditions. The flexible nature of the motor maps necessitates the reconsideration of the results of motor control and neuroplasticity studies. Neural mechanisms regulating forelimb muscle representations in M1 by the hindlimb sensorimotor state warrant further exploration.
Intra-condensate demixing of TDP-43 inside stress granules generates pathological aggregates
Authors: Yan Xiao , Kuster David , Mohanty Priyesh , Nijssen Jik , Pombo-García Karina , Rizuan Azamat , Franzmann Titus M. , Sergeeva Aleksandra , Passos Patricia M. , George Leah , Wang Szu-Huan , Shenoy Jayakrishna , Danielson Helen L. , Honigmann Alf , Ayala Yuna M. , Fawzi Nicolas L. , Mittal Jeetain , Alberti Simon , Hyman Anthony A. 
Published: January 2024

SUMMARYCytosolic aggregation of the nuclear protein TDP-43 is associated with many neurodegenerative diseases, but the triggers for TDP-43 aggregation are still debated. Here, we demonstrate that TDP-43 aggregation requires a double event. One is up-concentration in stress granules beyond a threshold, and the other is oxidative stress. These two events collectively induce intra-condensate demixing, giving rise to a dynamic TDP-43 enriched phase within stress granules, which subsequently transitions into pathological aggregates. Mechanistically, intra-condensate demixing is triggered by local unfolding of the RRM1 domain for intermolecular disulfide bond formation and by increased hydrophobic patch interactions in the C-terminal domain. By engineering TDP-43 variants resistant to intra-condensate demixing, we successfully eliminate pathological TDP-43 aggregates in cells. We conclude that up-concentration inside condensates and simultaneous exposure to environmental stress could be a general pathway for protein aggregation, with intra-condensate demixing constituting a key intermediate step.
Tau-related reduction of glucose metabolism in mild cognitive impairment occurs independently of APOE ε4 genotype and is gradually modulated by β-amyloid
Authors: Carbonell Felix , McNicoll Carolann , Zijdenbos Alex P. , Bedell Barry J. ,   Alzheimer’s Disease Neuroimaging Initiative
Published: January 2024

AbstractBackgroundPET imaging studies have shown that spatially distributed measurements of β-amyloid are significantly correlated with glucose metabolism in Mild Cognitive Impairment (MCI) independently of the APOE ε4 genotype. In contrast, the relationship between tau and glucose metabolism at different stages of Alzheimer’s Disease (AD) has not been fully understood.ObjectiveWe hypothesize that spatially distributed scores of tau PET are associated with an even stronger reduction of glucose metabolism, independent of the APOE ε4 genotype and gradually modulated by β-amyloid.MethodsWe applied a cross-sectional statistical analysis to concurrent [18F]flortaucipir PET, [18F]florbetapir PET, and 2-[18F]fluoro-2-deoxyglucose (FDG) PET images from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) study. We employed a Singular Value Decomposition (SVD) approach to the cross-correlation matrix between tau and the FDG images, as well as between tau and β-amyloid PET images. The resulting SVD-based tau scores are associated with cortical regions where a reduced glucose metabolism is maximally correlated with distributed patterns of tau, accounting for the effect of spatially distributed β-amyloid.ResultsFrom a population of MCI subjects, we found that the SVD-based tau scores had their maximal spatial representation within the entorhinal cortex and the lateral inferior temporal gyrus, and were significantly correlated with glucose metabolism in several cortical regions, independently from the confounding effect of the β-amyloid scores and APOE ε4. Moreover, β-amyloid gradually modulated the association between tau and glucose metabolism.ConclusionsOur approach uncovered spatially distributed patterns of the tau-glucose metabolism relationship after accounting for the β-amyloid effects. We showed that the SVD-based tau scores have a strong relationship with decreasing glucose metabolism. By highlighting the more significant role of tau, rather than β-amyloid, on the reduction of glucose metabolism, our results could have important consequences in the therapeutic treatment of AD.
Targeting resident astrocytes attenuates neuropathic pain after spinal cord injury
Authors: Zhao Qing , Zhu Yanjing , Ren Yilong , Zhao Lijuan , Zhao Jingwei , Yin Shuai , Ni Haofei , Zhu Rongrong , Cheng Liming , Xie Ning 
Published: January 2024

AbstractAstrocytes derive from different lineages and play a critical role in neuropathic pain after spinal cord injury (SCI). Whether selective eliminating these main origins of astrocytes in lumbar enlargement could attenuate SCI-induced neuropathic pain remains unclear. Through transgenic mice injected with an adeno-associated virus vector and diphtheria toxin, astrocytes in lumbar enlargement were lineage traced, targeted and selectively eliminated. Pain-related behaviors were measured with an electronic von Frey apparatus and a cold/hot plate after SCI. RNA sequencing, bioinformatics analysis, molecular experiment and immunohistochemistry were used to explore the potential mechanisms after astrocyte elimination. Lineage tracing revealed that the resident astrocytes but not ependymal cells were the main origins of astrocytes-induced neuropathic pain. SCI induced mice to obtain significant pain symptoms and astrocyte activation in lumbar enlargement. Selective resident astrocytes elimination in lumbar enlargement could attenuate neuropathic pain and activate microglia. Interestingly, the type I interferons (IFNs) signal was significantly activated after astrocytes elimination, and the most activated Gene Ontology terms and pathways were associated with the type I IFNs signal which was mainly activated in microglia and further verified in vitro and in vivo. Furthermore, different concentrations of interferon and Stimulator of interferon genes (STING) agonist could activate the type I IFNs signal in microglia. These results elucidate that selectively eliminating resident astrocytes attenuated neuropathic pain associated with type I IFNs signal activation in microglia. Targeting type I IFNs signal is proven to be an effective strategy for neuropathic pain treatment after SCI.
Effectiveness of simulation-based learning in Malaysian higher education: a case study of MonsoonSIM
Authors: Mohd Khalil Aisyah , Lee Khai Loon , Kamaruzzaman Zetty Ain , Ong Chin An 
Published: January 2024

PurposeHigher education institutions (HEIs) face the formidable responsibility of equipping students with the requisite knowledge and skills essential for a successful transition into the professional workforce. In contemporary education, simulation-based learning (SBL) has emerged as a pivotal tool employed by HEIs to facilitate and enhance the learning experience. MonsoonSIM stands out as a notable simulation-based experiential learning platform, encompassing a wide spectrum of business processes. This study aims to investigate the effectiveness of SBL in Malaysian HEI, with a specific focus on utilizing MonsoonSIM to bolster students' knowledge and skills.Design/methodology/approachTo gather empirical evidence, an online survey questionnaire was methodically distributed to 254 students enrolled in Malaysian HEIs, employing purposive sampling techniques. A total of 114 valid responses were collected and subjected to rigorous analysis using SmartPLS4, leveraging the partial least squares structural equation modeling methodology.FindingsThe outcomes of this investigation shed light on the positive influence of marketing management knowledge on the effectiveness of SBL. However, it was observed that problem-solving and critical thinking skills, financial management and production management knowledge did not exhibit a statistically significant impact on the effectiveness of SBL.Originality/valueThis study contributes to the existing body of knowledge by offering valuable insights into how students engage with and derive learning outcomes from simulation-based educational tools. The findings underscore the pivotal role of integrating SBL into the broader pedagogical framework to enhance the overall learning experience.
The relationship between learning orientation, firm performance and market dynamism in MSMEs operating in technology parks in Poland: an empirical analysis
Authors: Karpacz Jarosław , Wojcik-Karpacz Anna 
Published: January 2024

PurposeThe authors analyzed the relationship between learning orientation (LO) and performance in micro, small and medium-sized enterprises (MSMEs) by investigating the moderating role of environmental dynamism to answer the need for systematic research of models between LO and firm performance (FP).Design/methodology/approachThe authors investigated the (in)direct relationship between LO and FP. The authors collected data from 182 MSMEs operating in technology parks (TPs) in Poland. The authors used two methods in the quantitative empirical research. The authors used linear regression models to test the hypotheses, which allowed for a global assessment of relationships among all analyzed variables. Dynamic capabilities (DCs) framework guided the study.FindingsThe study results show that FP benefits from LO-related behaviors. LO is an important stimulant of FP. Meanwhile, the authors did not classify market dynamism (MD) as a moderator of the LO-FP relationship.Research limitations/implicationsBy design, the authors surveyed only MSMEs open to participate in the survey, which potentially limits generalizability. Furthermore, future researchers may consider other types of strategic orientations (SOs) to further explain the impact of multiple SOs on FP in specific industries.Originality/valueThis article presents arguments that allow for recognizing LO as a strategic organizational factor shaping FP.
Structuring organizations for supporting knowledge management and nurturing dynamic capabilities
Authors: Gupta Bindiya , Achhnani Bhumika 
Published: January 2024

Purpose
Till now no study has been undertaken which test the knowledge management processes for creating dynamic capabilities on the basis of organization structures with interpersonal trust as an important variable. This paper serves as a preliminary study proposing an integrated conceptual model that unmistakably unifies the notions of knowledge management and dynamic capability.

Design/methodology/approach
Through an exhaustive literature review, the study explores the role of knowledge management in building dynamic capabilities within the organizations. Through the available literature, authors have attempted to study the relevance of knowledge management against the backdrop of Structuration theory.

Findings
Through their proposed framework, authors posit that the structural elements of an organization set the tone for knowledge management within the organization. Organizations face dynamic challenges from the external environment, and in absence of interpersonal trust the creation of dynamic capabilities becomes difficult.

Originality/value
First, the current study enriches the growing research interest in Knowledge management. Second, the study connects Knowledge management and interpersonal trust within the organizations, which in turn is influenced by the structure of the organization. Structures created in the organization decide the direction, quality and quantity of knowledge sharing within the organizations both through social systems and through formal reporting systems. Thus, this paper serves as a preliminary study proposing an integrated conceptual model that unmistakably unifies the notions of knowledge management and dynamic capability.
The impact of job burnout on job satisfaction and intention to change occupation among accountants: the mediating role of psychological well-being
Authors: Hameli Kujtim , Çollaku Lum , Ukaj Lekë 
Published: January 2024

Purpose
This study aims to investigate the impact of job burnout on job satisfaction and the intention to change occupation within the accounting profession. It also examines the mediating role of psychological well-being.

Design/methodology/approach
A descriptive research design was used in this study. Survey data were physically collected from 230 accounting employees in the private sector. Structural equation modeling was used to test the theoretical model.

Findings
The results showed that job burnout significantly affects psychological well-being and that psychological well-being significantly mediates the relationship between job burnout and job satisfaction, as well as between job burnout and the intention to change occupation.

Research limitations/implications
This study has significant implications for accounting organizations, suggesting the adoption of strategies to promote psychological well-being. These initiatives have the potential to enhance job satisfaction and reduce accountants’ intention to change their profession.

Originality/value
This study contributes to the existing literature by highlighting the mediating role of psychological well-being in linking job burnout with job satisfaction and the intention to change occupation among accounting professionals.