bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stability of a Two-Layer Quasigeostrophic Vortex over Axisymmetric Localized Topography
Authors: Benilov E. S. 
Published: March 2005

Abstract
The stability of a quasigeostrophic vortex over a radially symmetric topographic feature (elevation or depression) in a two-layer ocean on the f plane is examined. This article’s concern is with compensated vortices, that is, those in which the lower layer is at rest (the disturbances, however, are present in both layers). Through numerical solution of the linear normal-mode problem, it is demonstrated that a bottom elevation is a stabilizing influence for a cyclone and a destabilizing influence for an anticyclone, whereas a bottom depression acts in the opposite way. These conclusions are interpreted using an asymptotic theory developed for the case of a thin upper layer. It is demonstrated that an elevation moves the critical level of an unstable mode toward the periphery of the cyclone, which leads to its stabilization. Estimates based on realistic oceanic parameters show that stabilization occurs for relatively small topography (5%–15% of the lower layer’s depth).
The Usefulness of In-Flight Measurements of Space Count to Improve Calibration of the AVHRR Solar Reflectance Bands
Authors: Ignatov Alexander , Cao Changyong , Sullivan Jerry , Levin Robert , Wu Xiangqian , Galvin Roy 
Published: March 2005

Abstract
The solar reflectance bands (SRB; centered at λ1 = 0.63, λ2 = 0.83, and λ3A = 1.61 μm) of the Advanced Very High Resolution Radiometers (AVHRR) flown on board NOAA satellites are often referred to as noncalibrated in-flight. In contrast, the Earth emission bands (EEBs; centered at λ3B = 3.7, λ4 = 11, and λ5 = 12 μm) are calibrated using two reference points: deep space and the internal calibration targets. In the SRBs, measurements of space count (SC) are also available; however, historically they are not used to specify the calibration offset [zero count (ZC)], which does not even appear in the calibration equation. A regression calibration formulation is used instead, equivalent to setting the ZC to a constant, whose value is specified from prelaunch measurements.
The analyses below, supported by a review of the instrument design and a wealth of historical SC information, show that the SC varies in-flight and differs from its prelaunch value. It is therefore suggested that 1) the AVHRR calibration equation in the SRBs be reformulated to explicitly use the ZC, consistently with the EEBs; and 2) the value of ZC be specified from the onboard measurements of SC. The ZC formulation of the calibration equation is physically solid, and it minimizes human-induced calibration errors resulting from the use of a regression formulation with an unconstrained intercept. Specifying the calibration offset improves radiances, most notably at the low end of radiometric scale, and subsequently provides for more accurate vicarious determinations of the calibration slope (gain). These calibration improvements are important for the products derived from the AVHRR low radiances, such as aerosol over ocean, and are particularly critical when generating their long-term climate data records (CDRs).
Computation of Domain-Averaged Irradiance Using Satellite-Derived Cloud Properties
Authors: Kato Seiji , Rose Fred G. , Charlock Thomas P. 
Published: March 2005

Abstract
The respective errors caused by the gamma-weighted two-stream approximation and the effective thickness approximation for computing the domain-averaged broadband shortwave irradiance are evaluated using cloud optical thicknesses derived from 1 h of radiance measurements by the Moderate Resolution Imaging Spectrometer (MODIS) over footprints of Clouds and the Earth’s Radiant Energy System (CERES) instruments. Domains are CERES footprints of which dimension varies approximately from 20 to 70 km, depending on the viewing zenith angle of the instruments. The average error in the top-of-atmosphere irradiance at a 30° solar zenith angle caused by the gamma-weighted two-stream approximation is 6.1 W m−2 (0.005 albedo bias) with a one-layer overcast cloud where a positive value indicates an overestimate by the approximation compared with the irradiance computed using the independent column approximation. Approximately one-half of the error is due to deviations of optical thickness distributions from a gamma distribution and the other half of the error is due to other approximations in the model. The error increases to 14.7 W m−2 (0.012 albedo bias) when the computational layer dividing the cloud layer is increased to four. The increase is because of difficulties in treating the correlation of cloud properties in the vertical direction. Because the optical thickness under partly cloudy conditions, which contribute two-thirds of cloudy footprints, is smaller, the error is smaller than under overcast conditions; the average error for partly cloudy condition is −2.4 W m−2 (−0.002 albedo bias) at a 30° solar zenith angle. The corresponding average error caused by the effective thickness approximation is 0.5 W m−2 for overcast conditions and −21.5 W m−2 (−0.018 albedo bias) for partly cloudy conditions. Although the error caused by the effective thickness approximation depends strongly on the optical thickness, its average error under overcast conditions is smaller than the error caused by the gamma-weighted two-stream approximation because the errors at small and large optical thicknesses cancel each other. Based on these error analyses, the daily average error caused by the gamma-weighted two-stream and effective thickness approximations is less than 2 W m−2.
Arginase attenuates inhibitory nonadrenergic noncholinergic nerve-induced nitric oxide generation and airway smooth muscle relaxation
Authors: Maarsingh Harm , Tio Marieke A , Zaagsma Johan , Meurs Herman 
Published: March 2005

Abstract

Background
Recent evidence suggests that endogenous arginase activity potentiates airway responsiveness to methacholine by attenuation of agonist-induced nitric oxide (NO) production, presumably by competition with epithelial constitutive NO synthase for the common substrate, L-arginine. Using guinea pig tracheal open-ring preparations, we now investigated the involvement of arginase in the modulation of neuronal nitric oxide synthase (nNOS)-mediated relaxation induced by inhibitory nonadrenergic noncholinergic (iNANC) nerve stimulation.

Methods
Electrical field stimulation (EFS; 150 mA, 4 ms, 4 s, 0.5 – 16 Hz)-induced relaxation was measured in tracheal preparations precontracted to 30% with histamine, in the presence of 1 μM atropine and 3 μM indomethacin. The contribution of NO to the EFS-induced relaxation was assessed by the nonselective NOS inhibitor L-NNA (0.1 mM), while the involvement of arginase activity in the regulation of EFS-induced NO production and relaxation was investigated by the effect of the specific arginase inhibitor nor-NOHA (10 μM). Furthermore, the role of substrate availability to nNOS in EFS-induced relaxation was measured in the presence of various concentrations of exogenous L-arginine.

Results
EFS induced a frequency-dependent relaxation, ranging from 6.6 ± 0.8% at 0.5 Hz to 74.6 ± 1.2% at 16 Hz, which was inhibited with the NOS inhibitor L-NNA by 78.0 ± 10.5% at 0.5 Hz to 26.7 ± 7.7% at 8 Hz (P < 0.01 all). In contrast, the arginase inhibitor nor-NOHA increased EFS-induced relaxation by 3.3 ± 1.2-fold at 0.5 Hz to 1.2 ± 0.1-fold at 4 Hz (P < 0.05 all), which was reversed by L-NNA to the level of control airways in the presence of L-NNA (P < 0.01 all). Similar to nor-NOHA, exogenous L-arginine increased EFS-induced airway relaxation (P < 0.05 all).

Conclusion
The results indicate that endogenous arginase activity attenuates iNANC nerve-mediated airway relaxation by inhibition of NO generation, presumably by limiting L-arginine availability to nNOS.
Inhibition of breathing after surfactant depletion is achieved at a higher arterial PCO2 during ventilation with liquid than with gas
Authors: Rieger-Fackeldey Esther , Sindelar Richard , Jonzon Anders , Schulze Andreas , Sedin Gunnar 
Published: March 2005

Abstract
Background
Inhibition of phrenic nerve activity (PNA) can be achieved when alveolar ventilation is adequate and when stretching of lung tissue stimulates mechanoreceptors to inhibit inspiratory activity. During mechanical ventilation under different lung conditions, inhibition of PNA can provide a physiological setting at which ventilatory parameters can be compared and related to arterial blood gases and pH.

Objective
To study lung mechanics and gas exchange at inhibition of PNA during controlled gas ventilation (GV) and during partial liquid ventilation (PLV) before and after lung lavage.

Methods
Nine anaesthetised, mechanically ventilated young cats (age 3.8 ± 0.5 months, weight 2.3 ± 0.1 kg) (mean ± SD) were studied with stepwise increases in peak inspiratory pressure (PIP) until total inhibition of PNA was attained before lavage (with GV) and after lavage (GV and PLV). Tidal volume (Vt), PIP, oesophageal pressure and arterial blood gases were measured at inhibition of PNA. One way repeated measures analysis of variance and Student Newman Keuls-tests were used for statistical analysis.

Results
During GV, inhibition of PNA occurred at lower PIP, transpulmonary pressure (Ptp) and Vt before than after lung lavage. After lavage, inhibition of inspiratory activity was achieved at the same PIP, Ptp and Vt during GV and PLV, but occurred at a higher PaCO2 during PLV. After lavage compliance at inhibition was almost the same during GV and PLV and resistance was lower during GV than during PLV.

Conclusion
Inhibition of inspiratory activity occurs at a higher PaCO2 during PLV than during GV in cats with surfactant-depleted lungs. This could indicate that PLV induces better recruitment of mechanoreceptors than GV.
The effects of quercetin on SW480 human colon carcinoma cells: a proteomic study
Authors: Mouat Michael F , Kolli Kumar , Orlando Ronald , Hargrove James L , Grider Arthur 
Published: March 2005

Abstract

Background
High fruit and vegetable intake is known to reduce the risk of colon cancer. To improve understanding of this phenomenon the action of different phytochemicals on colon cells has been examined. One such compound is quercetin that belongs to the group known as flavonoids. The purpose of this study was to determine the influence of quercetin on the proteome of the SW480 human colon adenocarcinoma cell line, specifically to identify proteins that could be the molecular targets of quercetin in its amelioration of the progression of colon cancer. To this end, two-dimensional gel electrophoresis and mass spectrometry were used to identify proteins that underwent a change in expression following treatment of the cells with 20 μM quercetin. This could elucidate how quercetin may reduce the progression of colon cancer.

Results
Quercetin treatment of the SW480 human colon cancer cells was found to result in the decreased expression of three proteins and the increased expression of one protein. The identified proteins with decreased expression were type II cytoskeletal 8 keratin and NADH dehydrogenase Fe-S protein 3. The other protein with decreased expression was not identified. The protein with increased expression belonged to the annexin family.

Conclusion
Several proteins were determined to have altered expression following treatment with quercetin. Such changes in the levels of these particular proteins could underlie the chemo-protective action of quercetin towards colon cancer.
Human macrophages limit oxidation products in low density lipoprotein
Authors: Hultén Lillemor Mattsson , Ullström Christina , Krettek Alexandra , van Reyk David , Marklund Stefan L , Dahlgren Claes , Wiklund Olov 
Published: March 2005

Abstract
This study tested the hypothesis that human macrophages have the ability to modify oxidation products in LDL and oxidized LDL (oxLDL) via a cellular antioxidant defence system. While many studies have focused on macrophage LDL oxidation in atherosclerosis development, less attention has been given to the cellular antioxidant capacity of these cells.
Compared to cell-free controls (6.2 ± 0.7 nmol/mg LDL), macrophages reduced TBARS to 4.42 ± 0.4 nmol/mg LDL after 24 h incubation with LDL (P = 0.022). After 2 h incubation with oxLDL, TBARS were 3.69 ± 0.5 nmol/mg LDL in cell-free media, and 2.48 ± 0.9 nmol/mg LDL in the presence of macrophages (P = 0.034). A reduction of lipid peroxides in LDL (33.7 ± 6.6 nmol/mg LDL) was found in the presence of cells after 24 h compared to cell-free incubation (105.0 ± 14.1 nmol/mg LDL) (P = 0.005). The levels of lipid peroxides in oxLDL were 137.9 ± 59.9 nmol/mg LDL and in cell-free media 242 ± 60.0 nmol/mg LDL (P = 0.012). Similar results were obtained for hydrogen peroxide. Reactive oxygen species were detected in LDL, acetylated LDL, and oxLDL by isoluminol-enhanced chemiluminescence (CL). Interestingly, oxLDL alone gives a high CL signal. Macrophages reduced the CL response in oxLDL by 45% (P = 0.0016). The increased levels of glutathione in oxLDL-treated macrophages were accompanied by enhanced catalase and glutathione peroxidase activities.
Our results suggest that macrophages respond to oxidative stress by endogenous antioxidant activity, which is sufficient to decrease reactive oxygen species both in LDL and oxLDL. This may suggest that the antioxidant activity is insufficient during atherosclerosis development. Thus, macrophages may play a dual role in atherogenesis, i.e. both by promoting and limiting LDL-oxidation.
Witch-hunt
Authors: Drucker Ernest 
Published: March 2005

Abstract
Beginning two years ago, the US Dept of Health and Human Services began "special reviews" of all current research grants that involved harm reduction, sex and drugs, and continues its ban on funding of needle exchange. With Bush's second term, the campaign was extended to all US funded international programs that dealt with these issues and populations. And, most recently, the US has again undertaken to dominate the discourse within international organizations charged with drug control and AIDS policies – especially those of the UN. But the international harm reduction and human rights community is fighting back in several important ways, including "An Open Letter to the delegates of the Forty-eighth session of the Commission on Narcotic Drugs (CND) of the UN" prepared by a group of 334 well respected public health experts and human rights advocates, protesting U.S. pressure on the U.N. to withdraw its support from harm reduction. This editorial includes the letter and signatures as well as French, Spanish, and Russian versions of the letter as additional files.
Are decisions using cost-utility analyses robust to choice of SF-36/SF-12 preference-based algorithm?
Authors: Pickard A Simon , Wang Zhixiao , Walton Surrey M , Lee Todd A 
Published: March 2005

AbstractBackgroundCost utility analysis (CUA) using SF-36/SF-12 data has been facilitated by the development of several preference-based algorithms. The purpose of this study was to illustrate how decision-making could be affected by the choice of preference-based algorithms for the SF-36 and SF-12, and provide some guidance on selecting an appropriate algorithm.MethodsTwo sets of data were used: (1) a clinical trial of adult asthma patients; and (2) a longitudinal study of post-stroke patients. Incremental costs were assumed to be $2000 per year over standard treatment, and QALY gains realized over a 1-year period. Ten published algorithms were identified, denoted by first author: Brazier (SF-36), Brazier (SF-12), Shmueli, Fryback, Lundberg, Nichol, Franks (3 algorithms), and Lawrence. Incremental cost-utility ratios (ICURs) for each algorithm, stated in dollars per quality-adjusted life year ($/QALY), were ranked and compared between datasets.ResultsIn the asthma patients, estimated ICURs ranged from Lawrence's SF-12 algorithm at $30,769/QALY (95% CI: 26,316 to 36,697) to Brazier's SF-36 algorithm at $63,492/QALY (95% CI: 48,780 to 83,333). ICURs for the stroke cohort varied slightly more dramatically. The MEPS-based algorithm by Franks et al. provided the lowest ICUR at $27,972/QALY (95% CI: 20,942 to 41,667). The Fryback and Shmueli algorithms provided ICURs that were greater than $50,000/QALY and did not have confidence intervals that overlapped with most of the other algorithms. The ICUR-based ranking of algorithms was strongly correlated between the asthma and stroke datasets (r = 0.60).ConclusionSF-36/SF-12 preference-based algorithms produced a wide range of ICURs that could potentially lead to different reimbursement decisions. Brazier's SF-36 and SF-12 algorithms have a strong methodological and theoretical basis and tended to generate relatively higher ICUR estimates, considerations that support a preference for these algorithms over the alternatives. The "second-generation" algorithms developed from scores mapped from other indirect preference-based measures tended to generate lower ICURs that would promote greater adoption of new technology. There remains a need for an SF-36/SF-12 preference-based algorithm based on the US general population that has strong theoretical and methodological foundations.
HLA class I and II genotype of the NCI-60 cell lines
Authors: Adams Sharon , Robbins Fu-Meei , Chen Deborah , Wagage Devika , Holbeck Susan L , Morse Herbert C , Stroncek David , Marincola Francesco M 
Published: March 2005

AbstractSixty cancer cell lines have been extensively characterized and used by the National Cancer Institute's Developmental Therapeutics Program (NCI-60) since the early 90's as screening tools for anti-cancer drug development. An extensive database has been accumulated that could be used to select individual cells lines for specific experimental designs based on their global genetic and biological profile. However, information on the human leukocyte antigen (HLA) genotype of these cell lines is scant and mostly antiquated since it was derived from serological typing. We, therefore, re-typed the NCI-60 panel of cell lines by high-resolution sequence-based typing. This information may be used to: 1) identify and verify the identity of the same cell lines at various institutions; 2) check for possible contaminant cell lines in culture; 3) adopt individual cell lines for experiments in which knowledge of HLA molecule expression is relevant. Since genome-based typing does not guarantee actual surface protein expression, further characterization of relevant cell lines should be entertained to verify surface expression in experiments requiring correct antigen presentation.
Validation and test-retest reliability of the Royal Free Interview for Spiritual and Religious Beliefs when adapted to a Greek population
Authors: Sapountzi-Krepia Despina , Raftopoulos Vasilios , Sgantzos Marcos , Kotrotsiou Evangelia , Roupa-Darivaki Zoe , Sotiropoulou Kalliope , Ntourou Ioanna , Dimitriadou Alexandra 
Published: March 2005

Abstract
Background
The self-report version of the Royal Free Interview for Religious and Spiritual Beliefs has been confirmed as a valid and reliable scale, assessing the manner and nature in which spiritual beliefs are expressed. The aim of the present study was to evaluate the test-retest reliability and psychometric properties of the Greek version of the Royal Free Interview for Religious and Spiritual Beliefs.

Methods
A total of 209 persons (77 men and 132 women) with a mean age of 28.33 ± 9.44 years participated in the study (test group). We subsequently approached 139 participants of the test group with a mean age of 28.93 ± 9.60 years, who were asked to complete the Royal Free Questionnaire a second time two weeks later (retest group).

Results
The vast majority of participants (58.9%) reported both a religious and a spiritual belief, compared to 52 (25.1%) who told of a religious belief only. The internal consistency of the spiritual scale for the test group proved to be good, as standardized inter-item reliability / Cronbach's alpha was 0.83. Item-total correlations ranged from 0.51 to 0.73. They indicated very good levels of differentiation, thus showing that the questions were appropriate. Internal consistency of the spiritual scale for the retest group proved as good as for the test group. Standardized inter-item reliability / Cronbach's alpha was 0.84. Item-total correlations ranged from 0.52 to 0.75. The Pearson correlation coefficient for the total test-retest score of the spiritual scale was 0.754 (p < 0.001).

Conclusion
The Greek version of the Royal Free Interview for Religious and Spiritual Beliefs is reliable and thus suitable for use in Greece.
Signalling pathway involved in nitric oxide synthase type II activation in chondrocytes: synergistic effect of leptin with interleukin-1
Authors: Otero Miguel , Lago Rocío , Lago Francisca , Reino Juan Jesús Gomez , Gualillo Oreste 
Published: March 2005

AbstractThe objective of the present study was to investigate the effect of leptin, alone or in combination with IL-1, on nitric oxide synthase (NOS) type II activity in vitro in human primary chondrocytes, in the mouse chondrogenic ATDC5 cell line, and in mature and hypertrophic ATDC5 differentiated chondrocytes. For completeness, we also investigated the signalling pathway of the putative synergism between leptin and IL-1. For this purpose, nitric oxide production was evaluated using the Griess colorimetric reaction in culture medium of cells stimulated over 48 hours with leptin (800 nmol/l) and IL-1 (0.025 ng/ml), alone or combined. Specific pharmacological inhibitors of NOS type II (aminoguanidine [1 mmol/l]), janus kinase (JAK)2 (tyrphostin AG490 and Tkip), phosphatidylinositol 3-kinase (PI3K; wortmannin [1, 2.5, 5 and 10 μmol/l] and LY294002 [1, 2.5, 5 and 10 μmol/l]), mitogen-activated protein kinase kinase (MEK)1 (PD098059 [1, 5, 10, 20 and 30 μmol/l]) and p38 kinase (SB203580 [1, 5, 10, 20 and 30 μmol/l]) were added 1 hour before stimulation. Nitric oxide synthase type II mRNA expression in ATDC5 chondrocytes was investigated by real-time PCR and NOS II protein expression was analyzed by western blot. Our results indicate that stimulation of chondrocytes with IL-1 results in dose-dependent nitric oxide production. In contrast, leptin alone was unable to induce nitric oxide production or expression of NOS type II mRNA or its protein. However, co-stimulation with leptin and IL-1 resulted in a net increase in nitric oxide concentration over IL-1 challenge that was eliminated by pretreatment with the NOS II specific inhibitor aminoguanidine. Pretreatment with tyrphostin AG490 and Tkip (a SOCS-1 mimetic peptide that inhibits JAK2) blocked nitric oxide production induced by leptin/IL-1. Finally, wortmannin, LY294002, PD098059 and SB203580 significantly decreased nitric oxide production. These findings were confirmed in mature and hypertrophic ATDC5 chondrocytes, and in human primary chondrocytes. This study indicates that leptin plays a proinflammatory role, in synergy with IL-1, by inducing NOS type II through a signalling pathway that involves JAK2, PI3K, MEK-1 and p38 kinase.