bluuebunny/crossref_metadata_2025_split huggingface dataset:
Poor sleep and shift work associate with increased blood pressure and inflammation in UK Biobank participants
Authors: Kanki Monica , Nath Artika P. , Xiang Ruidong , Yiallourou Stephanie , Fuller Peter J. , Cole Timothy J. , Cánovas Rodrigo , Young Morag J. 
Published: November 2023

AbstractDisrupted circadian rhythms have been linked to an increased risk of hypertension and cardiovascular disease. However, many studies show inconsistent findings and are not sufficiently powered for targeted subgroup analyses. Using the UK Biobank cohort, we evaluate the association between circadian rhythm-disrupting behaviours, blood pressure (SBP, DBP) and inflammatory markers in >350,000 adults with European white British ancestry. The independent U-shaped relationship between sleep length and SBP/DBP is most prominent with a low inflammatory status. Poor sleep quality and permanent night shift work are also positively associated with SBP/DBP. Although fully adjusting for BMI in the linear regression model attenuated effect sizes, these associations remain significant. Two-sample Mendelian Randomisation (MR) analyses support a potential causal effect of long sleep, short sleep, chronotype, daytime napping and sleep duration on SBP/DBP. Thus, in the current study, we present a positive association between circadian rhythm-disrupting behaviours and SBP/DBP regulation in males and females that is largely independent of age.
Two subtypes of GTPase-activating proteins coordinate tip growth and cell size regulation in Physcomitrium patens
Authors: Ruan Jingtong , Lai Linyu , Ou Hongxin , Yi Peishan 
Published: November 2023

AbstractThe establishment of cell polarity is a prerequisite for many developmental processes. However, how it is achieved during tip growth in plants remains elusive. Here, we show that the RHO OF PLANTs (ROPs), ROP GUANINE NUCLEOTIDE EXCHANGE FACTORs (RopGEFs), and ROP GTPASE-ACTIVATING PROTEINs (RopGAPs) assemble into membrane domains in tip-growing cells of the moss Physcomitrium patens. The confinement of membrane domains requires redundant global inactivation of ROPs by PpRopGAPs and the PLECKSTRIN HOMOLOGY (PH) domain-containing RenGAP PpREN. Unexpectedly, PpRopGAPs and PpREN exert opposing effects on domain size and cell width upon overexpression. Biochemical and functional analyses indicate that PpRopGAPs are recruited to the membrane by active ROPs to restrict domain size through clustering, whereas PpREN rapidly inactivates ROPs and inhibits PpRopGAP-induced clustering. We propose that the activity- and clustering-based domain organization by RopGAPs and RenGAPs is a general mechanism for coordinating polarized cell growth and cell size regulation in plants.
Mutational spectra are associated with bacterial niche
Authors: Ruis Christopher , Weimann Aaron , Tonkin-Hill Gerry , Pandurangan Arun Prasad , Matuszewska Marta , Murray Gemma G. R. , Lévesque Roger C. , Blundell Tom L. , Floto R. Andres , Parkhill Julian 
Published: November 2023

AbstractAs observed in cancers, individual mutagens and defects in DNA repair create distinctive mutational signatures that combine to form context-specific spectra within cells. We reasoned that similar processes must occur in bacterial lineages, potentially allowing decomposition analysis to detect both disruption of DNA repair processes and exposure to niche-specific mutagens. Here we reconstruct mutational spectra for 84 clades from 31 diverse bacterial species and find distinct mutational patterns. We extract signatures driven by specific DNA repair defects using hypermutator lineages, and further deconvolute the spectra into multiple signatures operating within different clades. We show that these signatures are explained by both bacterial phylogeny and replication niche. By comparing mutational spectra of clades from different environmental and biological locations, we identify niche-associated mutational signatures, and then employ these signatures to infer the predominant replication niches for several clades where this was previously obscure. Our results show that mutational spectra may be associated with sites of bacterial replication when mutagen exposures differ, and can be used in these cases to infer transmission routes for established and emergent human bacterial pathogens.
Double boron–oxygen-fused polycyclic aromatic hydrocarbons: skeletal editing and applications as organic optoelectronic materials
Authors: Li Guijie , Xu Kewei , Zheng Jianbing , Fang Xiaoli , Yang Yun-Fang , Lou Weiwei , Chu Qingshan , Dai Jianxin , Chen Qidong , Yang Yuning , She Yuan-Bin 
Published: November 2023

AbstractAn efficient one-pot strategy for the facile synthesis of double boron–oxygen-fused polycyclic aromatic hydrocarbons (dBO-PAHs) with high regioselectivity and efficient skeletal editing is developed. The boron–oxygen-fused rings exhibit low aromaticity, endowing the polycyclic aromatic hydrocarbons with high chemical and thermal stabilities. The incorporation of the boron–oxygen units enables the polycyclic aromatic hydrocarbons to show single-component, low-temperature ultralong afterglow of up to 20 s. Moreover, the boron–oxygen-fused polycyclic aromatic hydrocarbons can also serve as ideal n-type host materials for high-brightness and high-efficiency deep-blue OLEDs; compared to single host, devices using boron–oxygen-fused polycyclic aromatic hydrocarbons-based co-hosts exhibit dramatically brightness and efficiency enhancements with significantly reduced efficiency roll-offs; device 9 demonstrates a high color-purity (Commission International de l’Eclairage CIEy = 0.104), and also achieves a record-high external quantum efficiency (28.0%) among Pt(II)-based deep-blue OLEDs with Commission International de l’Eclairage CIEy < 0.20; device 10 achieves a maximum brightnessof 27219 cd/m2 with a peak external quantum efficiency of 27.8%, which representes the record-high maximum brightness among Pt(II)-based deep-blue OLEDs. This work demonstrates the great potential of the double boron–oxygen-fused polycyclic aromatic hydrocarbons as ultralong afterglow and n-type host materials in optoelectronic applications.
Transcriptional repression of beige fat innervation via a YAP/TAZ-S100B axis
Authors: Huang Xun , Li Xinmeng , Shen Hongyu , Zhao Yiheng , Zhou Zhao , Wang Yushuang , Yao Jingfei , Xue Kaili , Wu Dongmei , Qiu Yifu 
Published: November 2023

AbstractSympathetic innervation is essential for the development of functional beige fat that maintains body temperature and metabolic homeostasis, yet the molecular mechanisms controlling this innervation remain largely unknown. Here, we show that adipocyte YAP/TAZ inhibit sympathetic innervation of beige fat by transcriptional repression of neurotropic factor S100B. Adipocyte-specific loss of Yap/Taz induces S100b expression to stimulate sympathetic innervation and biogenesis of functional beige fat both in subcutaneous white adipose tissue (WAT) and browning-resistant visceral WAT. Mechanistically, YAP/TAZ compete with C/EBPβ for binding to the zinc finger-2 domain of PRDM16 to suppress S100b transcription, which is released by adrenergic-stimulated YAP/TAZ phosphorylation and inactivation. Importantly, Yap/Taz loss in adipocytes or AAV-S100B overexpression in visceral WAT restricts both age-associated and diet-induced obesity, and improves metabolic homeostasis by enhancing energy expenditure of mice. Together, our data reveal that YAP/TAZ act as a brake on the beige fat innervation by blocking PRDM16-C/EBPβ-mediated S100b expression.
Next generation sequencing uncovers multiple miRNAs associated molecular targets in gallbladder cancer patients
Authors: Saxena Rahul , Chakrapani Baskar , Sarath Krishnan M. P. , Gupta Amit , Gupta Sweety , Das Jayanta , Gupta Subash C. , Mirza Anissa A. , Rao Shalinee , Goyal Bela 
Published: November 2023

Abstract
Gallbladder cancer (GBC) is a lethal disease with surgical resection as the only curative treatment. However, many patients are ineligible for surgery, and current adjuvant treatments exhibit limited effectiveness. Next-generation sequencing has improved our understanding of molecular pathways in cancer, sparking interest in microRNA-based gene regulation. The aim of the study is to identify dysregulated miRNAs in GBC and investigate their potential as therapeutic tools for effective and targeted treatment strategies. GBC and control tissue samples were sequenced for miRNA expression using the Illumina HiSeq platform. Biological processes and related pathways were determined using the Panther and Gene Ontology databases. 439 significantly differentially expressed miRNAs were identified; 19 of them were upregulated and 29 were downregulated. Key enriched biological processes included immune cell apoptosis, endoplasmic reticulum (ER) overload response, and negative regulation of the androgen receptor (AR) signaling pathway. Panther analysis revealed the insulin-like growth factor (IGF)-mitogen activated protein kinases (MAPK) cascade, p38 MAPK pathway, p53 pathway, and FAS (a subgroup of the tumor necrosis factor receptor) signaling pathway as highly enriched among dysregulated miRNAs. Kirsten rat sarcoma virus (KRAS), AR, and interferon gamma (IFN-γ) pathways were identified among the key pathways potentially amenable to targeted therapy. We concluded that a combination approach involving miRNA-based interventions could enhance therapeutic outcomes. Our research emphasizes the importance of precision medicine, targeting pathways using sense and anti-sense miRNAs as potential therapies in GBC.
Comprehensive analysis of genes associated with migraine in the Indian population: a meta-analysis of genetic association studies with trial sequential analysis
Authors: Sudershan Amrit , Pushap Agar Chander , Bhagat Meenakshi , Sharma Isha , Kumar Hardeep , Digra Sanjeev K. , Kumar Parvinder 
Published: November 2023

AbstractMigraine is a complex disorder with multigenic inheritance and is characterized by the cardinal symptom of unilateral headache. Many genes are responsible for increasing the susceptibility of disease within different populations. Therefore, our primary aim in this review was to catalog the many genes that have been studied in India and after collecting the necessary information, we calculated a more precise risk relationship between an identified variation and migraine. The gene and its associated risk variant were discovered in the Indian population using a PRISMA-based systematic literature review guideline from online databases such as PubMed & Google Scholar. We constructed pooled odds ratios with 95% confidence intervals using multiple genetic models. Also, we looked for heterogeneity using Cochran's Q Test and the I2 statistic. Publication bias was analyzed using Begg's and Egger's tests. A p-value less than 0.05 was judged to be statistically significant for all tests. After a critical analysis, a total of 24 studies explored about 21 genes with 31 variants out of which only nine genes have been studied more than two times in the Indian population and thus were found eligible for the meta-analysis. It has been found, that the ACE-DD variant (allele model: OR: 1.37 [1.11–1.69], I2 = 0%/ fixed model), ESR1-PvuII (allele model: OR: 1.47 [1.24–1.74], I2 = 0%/ fixed model) significantly increases the risk of migraine in Indian population. Also, a protective role of the LRP1-rs11172113variant was observed for both migraine and its clinical subtype i.e., MA (allelic model: OR of 0.65 [0.50–0.83] I2 = 44% and allele: OR: 0.54 [0.37–0.78], I2 = 52%) respectively. Overall, the results of this meta-analysis indicated that the ACE-DD variant and the ESR1-PvuII were associated with an increased risk of migraine in the Indian community, while the LRP1-rs11172113 variant was associated with protection from migraine in this population.
Experimental investigation and multi-performance optimization of the leachate recirculation based sustainable landfills using Taguchi approach and an integrated MCDM method
Authors: Khan Osama , Mufazzal Sameera , Sherwani Ahmad F. , Khan Zahid A. , Parvez Mohd , Idrisi Mohammad Javed 
Published: November 2023

AbstractLandfill leachates contain harmful substances viz. chemicals, heavy metals, and pathogens, that pose a threat to human health and the environment. Unattended leachate can also cause ground water contamination, soil pollution and air pollution. This study focuses on management of leachate, by recirculating the rich, nutrient-filled fluid back into the landfills, turning it to a bioreactor, thereby maximising the performance parameters of landfills favourable for electricity production by the waste to energy plants. This study demonstrates a sustainable alternative method for utilising the fluid, rather than treating it using an extremely expensive treatment process. Further, it also experimentally investigates the effect of varying levels of five input parameters of the landfill including waste particle size, waste addition, inorganic content in waste, leachate recirculation rate, and landfill age, each at five levels, on the multiple performance of the landfill using Taguchi’s L25 standard orthogonal array. Experimental results are analysed using an integrated MCDM approach i.e. MEREC-PIV method and statistical techniques such as analysis of mean (ANOM) and analysis of variance (ANOVA). The results indicate that the optimal setting of the input parameters is waste particle size at 9 ppm, waste addition at 80 Ktoe, inorganic content in waste at 2%, leachate recirculation rate at 250 l/day and landfill age at 3 years. Further, inorganic content waste is found to be the most significant parameter for the multiple performance of the landfill. This study presents a novel approach to produce input parameters for power plants which may enhance their profitability and sustainability.
Advances in the greener synthesis of chromopyrimidine derivatives by a multicomponent tandem oxidation process
Authors: Ghamari Kargar Pouya , Bagherzade Ghodsieh 
Published: November 2023

AbstractA hydrophilic cobalt/copper heterogeneous bimetallic catalyst named mTEG-CS-Co/Cu-Schiff-base/IL was successfully synthesized from chitosan polysaccharide. The new catalyst was investigated and confirmed using various techniques including FT-IR, FE-SEM, EDX-EDS, XRD, TEM, TGA, AFM, NMR and ICP. The catalyst exhibited powerful catalyst activity for the tandem one pot oxidative chromopyrimidine reaction from benzyl alcohols under mild conditions, utilizing air as a clean source in a green protocol. The catalyst was compatible with a wide range of benzyl alcohols, and aldehydes formed in situ, and bis-aldehydes synthesized were condensed with urea/4‑hydroxycumarin to provide favorable products in good yields for all derivatives (14 new derivatives). The presence of tri-ethylene glycol and imidazolium moieties with hydrophilic properties on the mTEG-CS-Co/Cu-Schiff-base/IL nanohybrid provides dispersion of the nanohybrid particles in water, leading to higher catalytic performance. Furthermore, the reaction exhibited several other notable features, including low catalyst loading, the ability to be recycled for up to 6 stages, high atom economy, a simple work procedure, short reaction time, utilization of an environmentally friendly nanohybrid, and the replacement of volatile and organic solvents with water solvent.
Red clays indicate sub-aerial exposure of the Rio Grande Rise during the Eocene volcanic episode
Authors: Srivastava Priyeshu , J. Murton Bramley , Sant’Anna Lucy Gomes , Florindo Fabio , Hassan Muhammad Bin , Taciro Mandacaru Guerra Julia , de Assis Janasi Valdecir , Jovane Luigi 
Published: November 2023

AbstractAutonomous underwater vehicle (AUV) mapping of the western Rio Grande Rise (RGR), South Atlantic, and subsequent exploration and photography of horizontal lava flows exposed in near vertical, faulted escarpments, showed occurrences of red clays/weathered volcanic tops trapped between successive alkaline lava flows. These red clays indicate a hiatus in successive volcanic eruptions. Here, we report detailed mineralogical, geochemical, and rock magnetic characteristics of one such distinct red clay dredged from ~ 650 m water depth in the western RGR. The mineral constituents of the red clay are kaolinite, magnetite, oxidized magnetite (/maghemite), hematite, and goethite, with biogenic calcite and halite occupying voids or precipitated on the surface of the red clay. The chemical index of alteration (CIA) has a value of 93, showing that red clay is a product of extreme chemical weathering of the lava flows. The alkaline volcanic rocks recovered from nearby show an age of ~ 44 Ma, indicating an Eocene age for the volcanism. We show that the red clays are a product of sub-aerial chemical weathering of these Eocene volcanic rocks, in a warm-wet climate, before the thermal subsidence of the RGR to its modern-day bathymetric depth.
Analysis on the synergistic variation law of pile refreezing process and bearing capacity based on high-strain dynamic test method
Authors: Yu Dezhong , Cao Yang , Zhao Qianqian 
Published: November 2023

AbstractThe high-strain dynamic test method possesses the attributes of speed, efficiency, and environmentally-friendly, low-carbon characteristics. To study the reliability of the high-strain dynamic test method in detecting the bearing capacity of pile foundation in permafrost regions, 4 test piles, each with a length of 15 m and a diameter of 1.0 m, were poured based on the actual bridge construction project in the permafrost region of Daxing'an Mountains in China. Based on the temperature data between the piles and soil, the refreezing state of the pile foundation was comprehensively judged. Subsequently, the static method was employed to assess the friction resistance values and single pile bearing capacity of each soil layer on the pile side under different freezing states before and after the pile foundation refreezing. Building upon these findings, the restrictive parameters for soil elastic limit $$q_{(i)}$$

q

(
i
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, soil resistance $$R_{U} (i)$$

R
U

(
i
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and other parameters in the pile-soil model of high-strain dynamic test method are clarified. The test and analysis results indicate that under the condition that the ground temperature of frozen soil is about − 1.9 °C, the pile-soil refreezing takes about 120 days, and the pile-soil refreezing is a slow process; In CAPWAPC calculation program, after the values of soil parameters were constrained by the results of static-load test, the calculated curve is in satisfactory agreement with the measured curve, and the parameters of the pile-soil model are reliable, which can be used for the rapid detection of the ultimate bearing capacity of piles in permafrost regions; In the process of pile-soil refreezing, the change of temperature field affects the freezing strength between pile and soil, the process of pile-soil refreezing is positively correlated with the increase of pile foundation bearing capacity.
Comprehensive characterization of TGFB1 across hematological malignancies
Authors: Wang Cui-zhu , Zhang Zi-qi , Zhang Yan , Zheng Liang-feng , Liu Yang , Yan Ai-ting , Zhang Yuan-cui , Chang Qing-hua , Sha Suo , Xu Zi-jun 
Published: November 2023

AbstractTGFB1, which encodes TGF-β1, a potent cytokine regulating varies cellular processes including immune responses. TGF-β1 plays context-dependent roles in cancers and is increasingly recognized as a therapeutic target to enhance immunotherapy responses. We comprehensively evaluated expression of TGFB1 and its clinical and biological effects across hematological malignancies. TGFB1 expression was first explored using data from the GTEx, CCLE, and TCGA databases. The expression and clinical significances of TGFB1 in hematological malignancies were analyzed using Hemap and our In Silico curated datasets. We also analyzed the relationship between TGFB1 with immune scores and immune cell infiltrations in Hemap. We further assessed the value of TGFB1 in predicting immunotherapy response using TIDE and real-world immunotherapy datasets. TGFB1 showed a hematologic-tissue-specific expression pattern both across normal tissues and cancer types. TGFB1 expression were broadly dysregulated in blood cancers and generally associated with adverse prognosis. TGFB1 expression were associated with distinct TME properties among different blood cancer types. In addition, TGFB1 expression was found to be a useful marker in predicting immunotherapy responses. Our results suggest that TGFB1 is broadly dysregulated in hematological malignancies. TGFB1 might regulate the immune microenvironment in a cancer-type-specific manner, which could be applied in the development of new targeted drugs for immunotherapy.
Surgical outcomes of acetabular fracture of elderly patients with superomedial dome impaction
Authors: Lim Eic Ju , Shon Hyun-Chul , Yang Jae-Young , Ahn Joosuk , Kim Jung Jae , Kim Ji Wan 
Published: November 2023

AbstractThis study aimed to investigate the outcomes of elderly acetabular fractures according to the reduction of impacted dome fragments. A retrospective cohort study was performed in two institutions. Fifty-four patients aged ≥ 60 years with acetabular fractures were enrolled. Data for dome impaction and postoperative reduction was collected. Patients were divided into the good reduction group (displacement ≤ 3 mm) and poor reduction group (displacement > 3 mm). Postoperative osteoarthritis (OA), Harris hip score (HHS), total hip arthroplasty conversion, good/poor outcomes were compared between the two groups. The good reduction group (N = 45) demonstrated a lower proportion of radiographic OA (18 vs. 77%, P = 0.001), higher HHS (82.1 vs. 68.6, P = 0.022), and higher proportion of good outcomes than the poor reduction group (N = 9) (89 vs. 22%, P < 0.001). In a subgroup analysis of the patients with dome impaction, the good reduction group had a higher proportion of good outcomes (80 vs. 20%, P = 0.031). On comparing within the good reduction group, dome impaction did not influence clinical outcomes. Elderly acetabular fractures demonstrated favorable outcomes when adequate reduction was achieved even with dome impaction. Well-reduced dome impaction could achieve satisfactory outcomes in elderly acetabular fractures.
Molecular and biological investigating of tea plant necrotic ring blotch virus as a worldwide threat
Authors: Esmaeilzadeh Fereshteh , Ghorbani Abozar , Koolivand Davoud 
Published: November 2023

AbstractTea plant necrotic ring blotch virus (TPNRBV) has emerged as a significant threat to tea plantations, primarily in China. Since 2020, similar symptoms have been observed in tea plants in northern Iran, raising concerns about the spread of this viral infection. In this study, we conducted an extensive investigation involving approximately 70 samples collected from both symptomatic and asymptomatic tea plants. Using reverse transcription-polymerase chain reaction with specially designed primers, we successfully amplified DNA fragments from 26 samples, confirming the presence of TPNRBV. Subsequent sequencing of these fragments revealed various segments of the TPNRBV genome. Our phylogenetic analysis revealed that the Iranian TPNRBV isolates formed a distinct sub-cluster alongside Chinese isolates, distinguishing them from Japanese isolates. These finding sheds light on the genetic diversity and relationships of TPNRBV across different regions. Additionally, we explored the potential modes of TPNRBV transmission. Mechanical transmission experiments confirmed the ability of the virus to infect Nicotiana rustica and Chenopodium quinoa seedlings, highlighting the risk of mechanical spread within tea plantations. Moreover, we investigated seed transmission and found evidence of TPNRBV in various parts of tea seeds, suggesting the possibility of seed-borne transmission. Overall, this comprehensive study enhances our understanding of the biological and molecular characteristics of TPNRBV, an emerging threat to global tea production. Our findings provide valuable insights into the virus’s transmission dynamics and genetic diversity, which are essential for developing effective management strategies to mitigate its impact on tea cultivation worldwide.