bluuebunny/crossref_metadata_2025_split huggingface dataset:
IL-8 is a novel prometastatic chemokine in intrahepatic cholangiocarcinoma that induces CXCR2-PI3K/AKT signaling upon CD97 activation
Authors: Meng Ze-Wu , Zhang Lei , Cai Xin-Ran , Wang Xing , She Fei-Fei , Chen Yan-Ling 
Published: October 2023

AbstractIntrahepatic cholangiocarcinoma (ICC) is a rare but highly aggressive malignant tumor arising within the liver, with a 5-year survival rate of only 20–40% after surgery. The role of interleukin-8 (IL-8) in ICC progression remains elusive. A transcriptomic approach based on IL-8 stimulation first revealed significant upregulation of the prometastatic gene CD97 and key epithelial–mesenchymal transition (EMT) factors E-cadherin and vimentin. Immunohistochemistry of 125 ICC tissues confirmed the positive correlation between IL-8 and CD97. Multivariable Cox regression indicated that they are both independent predictors of ICC prognosis. Mechanistically, IL-8 treatment induced CD97 expression at 50 and 100 ng/ml in QBC-939 and QBE cells, respectively. Moreover, the induction of cell migration and invasion upon IL-8 treatment was attenuated by CD97 RNA interference, and the expression of EMT-associated genes was dramatically inhibited. To determine whether CXCR1 or CXCR2 are downstream effectors of IL-8, siCXCR2 was applied and shown to significantly attenuate the oncogenic effects of IL-8 by inhibiting the phosphorylation of PI3K/AKT. Finally, the induction of CD97 expression by the PI3K pathway was verified by treatment with the inhibitor LY294002. In vivo, the significant tumor growth and lung metastasis effects induced by intraperitoneal injection of IL-8 were greatly inhibited by silencing CD97 in nude mice. Collectively, the study presents a novel mechanism of the IL-8-CXCR2-PI3K/AKT axis in regulating CD97 expression, which leads to ICC metastasis mainly through EMT. The study may provide alternatives for targeting the tumor microenvironment in metastatic ICC.
Effects of assessment method (real-time versus video-recorded) on a validated pain-altered behavior scale used in castrated piglets
Authors: Trindade Pedro Henrique Esteves , Lopez-Soriano Magdiel , Merenda Victoria Rocha , Tomacheuski Rubia Mitalli , Pairis-Garcia Monique Danielle 
Published: October 2023

AbstractWe aimed to compare two assessment methodologies (real-time vs. video-recorded) using the Unesp-Botucatu Pig Composite Acute Pain Scale (UPAPS) in piglets before and after castration. Twenty-nine male piglets were castrated. Four observers scored the UPAPS over three perioperative timepoints of castration following two assessment methodologies. In real-time assessments, the observers were in-person observing the piglets in front of the pen. After two weeks, the observers did video-recorded assessments randomizing piglets and timepoints. Modeling was conducted to compare the UPAPS and each pain-altered behavior between methodologies. Intraclass correlation coefficient (ICC), Bland–Altman, and Lin’s concordance correlation coefficient (CCC) were conducted to investigate agreement between methodologies. UPAPS was statistically equivalent between methodologies (P = 0.4371). The ICC for each method was very good (0.85 to 0.91). The agreement of the UPAPS assessed between methodologies had minimal bias (− 0.04), no proportion bias, and 53% of the assessments presented a perfect agreement. However, CCC of the UPAPS was moderate (0.65), and only one pain-altered behavior (“presents difficulty in overcoming obstacles or other animals”) occurred more in real-time assessments (P = 0.0444). In conclusion, piglet pain assessment by UPAPS can be conducted in real-time based on a suitable agreement between the real-time and video-recorded assessment methods.
Lifetime enhancement in QDLEDs via an electron-blocking hole transport layer
Authors: Samaeifar Fatemeh , Azadinia Mohsen , Aziz Hany 
Published: October 2023

AbstractThis study investigates the impact of an engineered hole transport layer (HTL) on the stability of electroluminescent quantum dot light-emitting devices (QDLEDs). The 9-Phenyl-3,6-bis(9-phenyl-9Hcarbazol-3-yl)-9H-carbazole (Tris-PCz) HTL, which possesses a shallower lowest unoccupied molecular orbital (LUMO) energy level compared to the widely used 4,4′-bis(N-carbazolyl)-1,1′-biphenyl (CBP) HTL, is employed to confine electron overflow toward the HTL. Utilizing the Tris-PCz HTL results in a 20× improvement in the electroluminescence half-life (LT50) of QDLEDs compared with conventional QDLEDs using the CBP HTL. Electric and optoelectronic analyses reveal that the migration of excess electrons toward the HTL is impeded by the up-shifted LUMO level of Tris-PCz, contributing to prolonged operational device stability. Furthermore, the augmented electric field at the QD/Tris-PCz interface, due to accumulated electrons, expedites hole injection rates, leading to better charge injection balance and the confinement of the exciton recombination zone within the QD and thus the device stability enhancement. This study highlights the significant influence of the HTL on QDLED stability and represents one of the longest LT50 for a QDLED based on the conventional core/shell QD structure.
Temperate silvopastures provide greater ecosystem services than conventional pasture systems
Authors: Amorim Helen C. S. , Ashworth Amanda J. , O’Brien Peter L. , Thomas Andrew L. , Runkle Benjamin R. K. , Philipp Dirk 
Published: October 2023

AbstractManagement and design affect systems’ ability to deliver ecosystem services and meet sustainable intensification needs for a growing population. Soil–plant–animal health evaluations at the systems level for conventional and silvopastoral environments are lacking and challenge adoption across temperate regions. Impacts of silvopasture on soil quality, microclimate, cattle heat stress, forage quality and yield, and cattle weight gain were compared to a conventional pasture in the mid-southern US. Here, we illustrate silvopastures have greater soil organic carbon, water content, and overall quality, with lower temperatures (soil and cattle) than conventional pastures. Forage production and cattle weight gains were similar across systems; yet, conventional pasture systems would need approximately four times more land area to yield equivalent net productivity (tree, nuts, forage, and animal weight) of one ha of silvopasture. Temperate silvopastures enhanced delivery of ecosystem services by improving soil quality and promoting animal welfare without productivity losses, thus allowing sustainable production under a changing climate.
Exploration of semen quality analyzed by casa-mot systems of brahman bulls infected with BLV and BHV-1
Authors: Pichardo-Matamoros Derling , Sevilla Francisco , Elizondo-Salazar Jorge , Jiménez-Sánchez Carlos , Roldan Eduardo R. S. , Soler Carles , Gacem Sabrina , Valverde Anthony 
Published: October 2023

AbstractEnzootic bovine leukosis virus (BLV) and bovine herpesvirus 1 (BHV-1) are very important infectious agents for the livestock industry worldwide. The present study aimed to explore the association between natural exposure to BLV and BHV-1 with sperm quality analyzed by Computer-Assisted Semen Analysis (CASA) systems. Ten sexually mature Brahman bulls, with sanitary status BLV+/BHV-1+ (n = 2), BLV−/BHV-1+ (n = 6) and BLV-/BHV-1- (n = 2) were evaluated twice, 30 days apart. Results showed that sanitary status of each bull was not associated with semen quality. It was found that the quality of the semen from the second collection was better due to the interruption of sexual rest. The evidence thus revealed that a bull infected with BLV generated good-quality contaminated semen and, therefore, that it is essential to detect contaminated seminal samples to prevent the spread of BLV. A multivariate analysis showed the presence of four sperm subpopulations in Brahman bulls that differ significantly in their kinematic patterns and with respect to sanitary status (P < 0.05), indicating that infection-free and seronegative bulls present the best kinematic parameters, which improved discrimination of sperm quality according to sanitary status. Overall, the analyses indicate that the seropositive-infected bulls with BLV and BHV-1 should be excluded from beef cattle farms.
Safety assessment, radioiodination and preclinical evaluation of antinuclear antibody as novel medication for prostate cancer in mouse xenograft model
Authors: Nguyen Thu Minh Chau , Hoang Lu Duc Chinh , Nguyen Thi Khanh Giang , Nguyen Thi Ngoc , Nguyen Quang Chien , Nguyen Thanh Binh , Dang Ho Hong Quang , Bui Van Cuong , Pham Thanh Minh , Nguyen Thi Thu 
Published: October 2023

AbstractThis study aims to provide in vitro and in vivo data to support the utilization of antinuclear antibodies (ANAs) as novel tools for the diagnosis and treatment of prostate cancers. The hematological, biochemical, and histological toxicities of ANAs were assessed at the doses of 5 and 50 μg per mouse. Radiolabeling study was then conducted with ANA and 131I using the chloramine T method, and the biodistribution and treatment efficacy were subsequently investigated in a PC3 xenograft model. No changes in clinical behavior or signs of intoxication, necrosis, or malignancy were observed in ANA-treated mice. 131I-ANA was obtained in very high yield and radiochemical purity, at 94.97 ± 0.98% and 98.56 ± 0.29%, respectively. They achieved immunoreactivity fraction of 0.841 ± 0.17% with PC-3 cells. Levels of radiolabeled ANAs were 1.15–10.14 times higher in tumor tissues than in other examined organs at 24 h post-injection. The tumor growth inhibition rates were 28.33 ± 5.01% in PC3 xenografts mice treated with 131I-ANAs compared with controls and a nearly twofold improvement in median survival was observed. These results demonstrate that radioimmunotherapy of radiolabeled natural ANAs may be an effective treatment for prostate tumors.
Factors affecting fledglings survival in urban population of European blackbirds in Szczecin (NW Poland)
Authors: Wysocki Dariusz , Witkowska Marta , Walczakiewicz Szymon 
Published: October 2023

AbstractThe first-year survival alters population growth rates and viability in birds, however this period remains the least-studied of the avian life stages. Here we present results of the 19 years of study of fledglings apparent survival of urban population of European blackbirdTurdus merulain Szczecin (NW Poland). We checked for possible influence on survival of several factors, including parental traits, such as parental age, their previous breeding experience, natal brood size, presence of another brood in a given breeding season and the time gap between clutches of a particular pair. Moreover, we incorporate into our analysis fledging’s hierarchy in the brood, its fledging time in the breeding season, temperature and precipitation during the first months of life. We also investigated changes in the apparent survival over 19 years. We found that the individual’s hierarchy in the nest, and the day of fledging had the strongest influence on the apparent survival, with heavier birds fledged earlier in the season surviving better. Increase in parental age and previous breeding experience of the pair could result in enhanced survival. Surprisingly increased precipitation lowered fledglings’ survival. During the 19 years of the study fledglings’ apparent survival dropped about 10%.
Exploring the microbiome of two uterine sites in cows
Authors: Murga Valderrama Nilton Luis , Segura Portocarrero Gleni Tatiana , Romani Vasquez Ana Cecilia , Frias Torres Hugo , Flores Durand Gary Jacsel , Cornejo Villanueva Victor Guillermo , Del Solar Jakson Ch. , Costa Polveiro Richard , da Silva Vieira Dielson , Bardales Escalante William , Zamora-Huamán Segundo José , Ordinola-Ramirez Carla Maria , Maicelo Quintana Jorge Luis , Lopez Lapa Rainer Marco 
Published: October 2023

AbstractBacterial communities in the mammalian reproductive system can be rich and diverse, differing in structure and quantity depending on location. In addition, its microbiome is associated with the state of health of this tract and reproductive success. This study evaluated the microbiome composition of the uterine body (UB) and uterine horn mucosa (UH) samples using 16S rRNA sequencing of samples extracted from cows in the Amazon region. It was observed that four main phyla were shared between the uterine sites: Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Linear discriminant analysis effect size and heat tree analysis showed that members of Lachnospiraceae (NK3A20 group) and Oscillospiraceae were significantly more abundant in the UB than in UH. In addition, there are more unique genera in the UB than in the UH. A higher bacterial load in UB than in UH is expected because of the exposure to external factors of UB. However, comparing the site's communities through beta diversity did not generate well-defined clustering. Thus, it can be attributed to the closeness of the sites, which would make the niches similar ecologically and microbiologically. Therefore, this research provides knowledge to understand biomarkers in the prior reproduction period.
Projection of climate change impacts on extreme temperature and precipitation in Central Poland
Authors: Ghazi Babak , Przybylak Rajmund , Pospieszyńska Aleksandra 
Published: October 2023

AbstractClimate change is exacerbating the risk of the occurrence of extreme weather. This study has projected the change in mean and extreme climate conditions in Central Poland during near-future (2026–2050), mid-term (2051–2075), and far-future (2076–2100) periods under two climate-change scenarios in six General Circulation Models (GCMs) from Coupled Model Intercomparison Project Phase 6 (CMIP6). The results showed that, compared to the historical reference period (1990–2014), Central Poland will experience an increase in temperature and precipitation by the end of the twenty-first century. It is expected that the mean annual temperature and mean annual precipitation totals will increase by 1–4.8 °C and 2–7.5%, respectively. Furthermore, it is projected that the average number of hot, very hot days and extremely hot days (Tmax > 25 °C, > 30 °C, and > 35 °C), tropical nights (Tmin > 20 °C), and extremely high daily precipitation (> 10 mm, > 20 mm and > 30 mm) will also increase, while the average number of slight frost days (Tmin < 0 °C), and frost and severe frost days (Tmax < 0 °C, Tmax <  − 10 °C) will decline on average by the end of the twenty-first century. Therefore, it is essential for policymakers to take some appropriate measurements and strategies in advance to strengthen resilience to extreme climate events.
Autoavaliação de competências fundamentais e orientação médico durante o primeiro ano de residência ortopédica brasileira
Authors: Barbato Kelly Biancardini Gomes , Carvalho Luciana Santos de , Barreira Marangoni Viviani , Souza Fábio de , Vaena Marcella Martins de Vasconcelos 
Published: October 2023

Resumo
Objetivo A formação de um médico competente requer direcionar o perfil de pós-graduação residente para atividades práticas. Buscou-se identificar a orientação de relacionamento médico-paciente e a autoavaliação das competências fundamentais que eles apontaram que precisavam ser desenvolvidas.
Métodos Todos os 56 residentes em ortopedia admitidos entre 2016 e 2019 participaram do presente estudo observacional prospectivo. A Escala de Orientação Médico-Paciente (Patient Practitioner Orientation Scale [PPOS, na sigla em inglês]) e um questionário de autoavaliação foram respondidos no início e no final do primeiro ano de residência (R1) em Ortopedia e Traumatologia. Calculamos o desvio médio e padrão para itens e pontuações de PPOS e os analisamos através do teste t emparelhado. As opções de resposta do Questionário de Autoavaliação foram "sim" ou "preciso melhorar" e as habilidades foram classificadas na ordem decrescente da frequência das respostas "preciso melhorar" com descrição de número absoluto e percentual. Comparamos frequências usando o teste de Fisher. Consideramos significativos valores-p < 0,05. Os programas GraphPad Prism 8.4.3 (GraphPad Software, San Diego, CA, EUA) e Microsoft Excel (Microsoft Corporation, Redmond, WA, EUA) foram utilizados para análise estatística.
Resultados No período entre o início e o final do R1, a média total de PPOS diminuiu significativamente, de 4,63 para 4,50 (p = 0,024), mais focada em biomédica. Cerca de um terço dos residentes identificou competências do cuidado ao paciente, aprendizagem e melhoria baseadas na prática e habilidades interpessoais e de comunicação, como necessitando melhorar.
Conclusões As atividades de PPOS e autoavaliação podem promover práticas de reflexão e são possíveis ferramentas para avaliação de competência centrada no aluno. A orientação biomédica tende a prevalecer à medida que a formação dos médicos progride e as autoavaliações periódicas podem ser trabalhadas para construir uma mentalidade de crescimento.
Validação de constructo e experiência de uso de um simulador de artroscopia de ombro de baixo custo
Authors: Dau Leonardo , Almeida Paula Adamo , Kulcheski Alynson Larocca , Milcent Paul Andre , Filho Edmar Stieven 
Published: October 2023

Resumo
Objetivo Validar o modelo de baixo custo para treinamento em artroscopia e analisar a aceitação e utilidade do simulador desenvolvido no ensino e treinamento médico.
Método Dez acadêmicos do curso de medicina, dez residentes do terceiro ano em ortopedia e dez cirurgiões de ombro realizaram tarefas pré determinadas em um simulador de ombro duas vezes. Os parâmetros utilizados foram o tempo para completar as tarefas, quantidade de olhares para as mãos, escore de GOALS (Global Operative Assessment of Laparoscopic Skills) e comparados entre os grupos e intragrupos. Uma escala de Likert adaptada foi aplicada abordando as impressões dos indivíduos acerca do simulador e de sua aplicabilidade.
Resultados Na comparação intergrupos, os cirurgiões de ombro tiveram melhores escores e tempos que os demais grupos. Quando as tarefas foram repetidas, o grupo de cirurgiões, teve uma melhora de 59% no tempo (p < 0,05), assim como no grupo de acadêmicos. No escore de GOALS os cirurgiões de ombro apresentaram escores consistentemente melhores que os demais grupos. E quando avaliamos a evolução do primeiro para o segundo teste, o grupo de cirurgiões e o grupo de acadêmicos tiveram melhora estatisticamente significante (p < 0,05). No quesito de lookdowns houve diminuição em todos os grupos. Houve consenso em que o simulador é útil no treinamento.
Conclusão O simulador desenvolvido permitiu a diferenciação entre indivíduos com diferentes níveis de treinamento em cirurgia artroscópica. Foi aceito por 100% dos participantes como uma ferramenta útil no treinamento cirúrgico artroscópico do ombro.
Caracterização da membrana induzida pela técnica de Masquelet em modelo murino de defeito ósseo segmentar
Authors: Guimarães João Antonio Matheus , Scorza Breno Jorge Braga , Machado Jamila Alessandra Perini , Cavalcanti Amanda dos Santos , Duarte Maria Eugênia Leite 
Published: October 2023

Resumo
Objetivo Reproduzir em modelo animal a técnica cirúrgica de Masquelet utilizada no tratamento de defeitos ósseos críticos e analisar as características da membrana formada em torno do cimento ósseo.
Métodos Um defeito crítico de 10mm foi realizado na diáfise femoral de 21 ratos Sprague-Dawley. Após a ressecção da porção central da diáfise o defeito foi estabilizado com fio de Kirschner introduzido pelo canal medular e com a interposição de espaçador de cimento ósseo. Após 2, 4, e 6 semanas do procedimento cirúrgico os animais foram eutanasiados e avaliados em radiografias do membro posterior quanto ao tamanho do defeito, o alinhamento e a estabilidade da osteossíntese. As membranas formadas em torno do espaçador foram submetidas a análise histológica para avaliação da espessura, da maturação do tecido conjuntivo e da densidade vascular.
Resultados Ao longo do tempo as membranas inicialmente constituídas por tecido conjuntivo frouxo foram substituídas por membranas representadas por tecido conjuntivo denso, rico em fibras colágenas espessas. Com seis semanas a espessura das membranas foi maior (565 ± 208μm) do que com quatro (186,9 ± 70,21μm, p = 0,0002) e duas semanas (252,2 ± 55,1μm, p = 0,001). Todas as membranas do tempo inicial apresentaram focos de diferenciação osteogênica que reduziram progressivamente ao longo do tempo.
Conclusão Além da função estrutural e protetora da membrana, suas características biológicas intrínsecas podem contribuir ativamente para a regeneração óssea. A atividade biológica atribuída pela presença de focos de osteogênese confere à membrana potencial de osteoindução que favorece as condições locais para a integração do enxerto ósseo.
Assessment of Learning Style Preferences of Saudi Nursing Students
Authors: Mariano Maria E.M. , Woodman Alexander , AlNaam Yaser , Al-Jamea Lamiaa H. , Al-Ansari Rehab Y. , Quiambao Jenifer V. 
Published: October 2023

Abstract
Objective In line with global trends, growing number of educational institutions in Saudi Arabia are developing their curricula based on the feedback and suggestions from their students to help improve the quality of teaching. This study aims to assess the differences in learning style preferences among female nursing students of Saudi Arabia.
Methods This cross-sectional study included 124 female nursing students who were asked to answer the culturally adapted Arabic version of visual, aural/auditory, read/write, and kinesthetic (VARK) survey. Data were analyzed with SPSS, version 23. The Wilks' lambda or multivariate analysis (MANOVA) was used to determine the relationship between the participants' learning preferences, sociodemographic characteristics, and year of study.
Results Nursing students who had visual learning preferences had a significantly different learning style as compared to those who preferred aural and kinesthetic learning (p < 0.001). On the contrary, there was not enough evidence to conclude that the same was true for students who preferred to read/write.
Conclusions Further research is required to explore the relationship between learning style preferences and learning outcomes with the inclusion of a larger sample size and representatives of two genders, males and females. The findings of the current research provide the foundation for adaptive learning by identifying the individual preferences in learning among the nursing students.
Precoce ou tardio? O protocolo de reabilitação mais adequado para lesão na região ‘terra de ninguém’: Metanálise com análise sequencial de ensaios – Tendências de 20 anos
Authors: Setiawan Celleen Rei , Karna Made Bramantya 
Published: October 2023

Resumo
Objetivo O objetivo deste estudo é analisar vários protocolos de reabilitação e determinar quais métodos produzem um melhor resultado.
Métodos Os relatórios dos bancos de dados foram pesquisados entre 1990 e 2020, usando PubMed, banco de dados da biblioteca Cochrane, Ovid, Medline e vários outros ensaios publicados. Uma análise estatística foi feita a partir do Review Manager e Trial Sequential Analysis (TSA).
Resultado A taxa média de re-ruptura é de 3,3% (n = 8) no protocolo combinado, e até 8% (n = 48) no protocolo de Movimento Ativo Controlado (MAC). As metanálises não encontraram diferença significativa entre Kleinert vs MAC na taxa de re-ruptura. Também não há diferença significativa entre Duran e MAC na taxa de re-ruptura. Na Trial Sequential Analysis (TSA), a curva z não cruza ambos os limites sequenciais de ensaio, será necessário um ensaio adicional com amostra maior. A TSA de contratura em flexão MAC vs Kleinert indicou que o protocolo MAC pode ser superior ao Kleinert para reduzir a incidência de contratura em flexão. Para a faixa de contratura média em flexão de 6,6% (n = 18) no MAC a 23,6% (n = 76) no protocolo Kleinert.
Conclusão A metanálise atual propôs que a técnica combinada resultará em menor incidência de re-ruptura e melhor resultado funcional em lesões da zona flexora II do que outras técnicas. O método MAC também resulta em menos contratura em flexão do que outros. No entanto, serão necessárias mais metanálises com estudos com amostras maiores para confirmar a conclusão desta revisão.