bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation method of sustainable development of plateau lakeside cities under the perspective of new development philosophy and its empirical research
Authors: Luo Yi , Jia Tingfang , Li Anlin , Yang Kun , Pei Xingfang , Peng Changqing , Chen Rixiang , Li Dingpu , Wang Qingqing 
Published: December 2023

IntroductionSustainable Development Goals (SDGs) is another binding target indicator system proposed by the United Nations after the Millennium Development Goals (MDGs). Scientific monitoring of the state of sustainable development of cities can provide a reference for the progress of the implementation of the SDGs, and also provide research support for the successful achievement of the sustainable development of cities around the world.MethodsAlthough the indicator system proposed by the United Nations has drawn a vision of global sustainable development and given an evaluation framework and methodology, it fails to and is unlikely to encompass the rich connotations of China's new development philosophy. Therefore, this study takes the new development philosophy as the fundamental guideline, refers to the SDGs assessment system, and constructs a theoretical and methodological framework for assessing the sustainable development of plateau lakeside cities from the five dimensions of "innovation, coordination, greenness, openness, and sharing", and carries out empirical investigation in the research area of Kunming City, a typical plateau lakeside city.MethodsIt is found that the sustainable development level of Kunming is at a medium level, with a relatively fast development speed, but the growth momentum has slowed down; the development level of each dimension is relatively low, with a slow development speed, showing a fluctuating upward trend; the change in the level of coordinated development shows two states of low and medium coordination, showing a steady increase; the contribution of the five philosophies of relevance is sorted as: "Openness&gt;Sharing&gt;Green&gt;Coordination&gt;Innovation", and openness is the most important factor affecting the sustainable development level of plateau lakeside cities.DiscussionThis study demonstrates the need to enhance the level of sustainable development of cities by exploring their internal trade-offs and potential internal contributions.
How green credit affects corporate environmental information disclosure: evidence from new energy listed companies in China
Authors: Geng Limin , Yin Wenxin , Wu Xiaoxia , Lu Xueyuan , Zhang Can 
Published: December 2023

IntroductionEnvironmental information disclosure is an important means to protect the ecological environment, and global climate change puts forward higher requirements for corporate environmental information disclosure. New energy companies play an important role in addressing climate change and environmental information disclosure, and should strengthen environmental information disclosure and actively participate in ecological environmental protection.MethodsThis paper selected Chinese new energy listed companies as the research sample to investigate the impact of green credit on corporate environmental information disclosure, and proposed and empirically tested the hypothesis on the mechanism of the effect of green credit on environmental information disclosure.ResultsThe regression results indicated that green credit can effectively improve the quality of corporate environmental information disclosure, and this conclusion is still significantly valid after robustness test; The impact mechanism tests showed that green credit can change the level of corporate environmental information disclosure by controlling company size. This paper also tested the heterogeneity and adjustment effects, showing that the relationship between green credit and environmental information disclosure are affected by multiple factors.DiscussionsCombining the research of this paper and previous research results, it is found that the positive impact of green credit on corporate environmental information disclosure has been confirmed by academic research and practical verification. However, the implementation effect of green credit policy will be affected by the economic level, regional differences, and the comprehensive impact from companies, financial institutions and government departments.
Biological analysis of the potential pathogenic mechanisms of Infectious COVID-19 and Guillain-Barré syndrome
Authors: Gao Hongyu , Wang Shuning , Duan Hanying , Wang Yushi , Zhu Hui 
Published: December 2023

BackgroundGuillain-Barré syndrome (GBS) is a medical condition characterized by the immune system of the body attacking the peripheral nerves, including those in the spinal nerve roots, peripheral nerves, and cranial nerves. It can cause limb weakness, abnormal sensations, and facial nerve paralysis. Some studies have reported clinical cases associated with the severe coronavirus disease 2019 (COVID-19) and GBS, but how COVID-19 affects GBS is unclear.MethodsWe utilized bioinformatics techniques to explore the potential genetic connection between COVID-19 and GBS. Differential expression of genes (DEGs) related to COVID-19 and GBS was collected from the Gene Expression Omnibus (GEO) database. By taking the intersection, we obtained shared DEGs for COVID-19 and GBS. Subsequently, we utilized bioinformatics analysis tools to analyze common DEGs, conducting functional enrichment analysis and constructing Protein–protein interaction networks (PPI), Transcription factors (TF) -gene networks, and TF-miRNA networks. Finally, we validated our findings by constructing the Receiver Operating Characteristic (ROC) curves.ResultsThis study utilizes bioinformatics tools for the first time to investigate the close genetic relationship between COVID-19 and GBS. CAMP, LTF, DEFA1B, SAMD9, GBP1, DDX60, DEFA4, and OAS3 are identified as the most significant interacting genes between COVID-19 and GBS. In addition, the signaling pathway of NOD-like receptors is believed to be essential in the link between COVID-19 and GBS.
Mulibrey nanism and immunological complications: a comprehensive case report and literature review
Authors: Gazzin Andrea , Pala Francesca , Bosticardo Marita , Niemela Julie , Stoddard Jennifer , Biasin Eleonora , Quarello Paola , Carli Diana , Ferroni Francesca , Delmonte Ottavia M. , Montin Davide , Rosenzweig Sergio D. , Licciardi Francesco , Notarangelo Luigi D. 
Published: December 2023

IntroductionMulibrey nanism (MUL) is a rare disorder caused by TRIM37 gene variants characterized by growth failure, dysmorphic features, congestive heart failure (CHF), and an increased risk of Wilms’ tumor. Although immune system impairment has been documented in MUL, the underlying mechanisms remain poorly understood.MethodsWe present a case of MUL with progressive lymphopenia and review similar cases from the literature.ResultsOur patient presented with prenatal onset growth restriction, characteristic dysmorphic features, and Wilms’ tumor. She developed progressive lymphopenia starting at 10 years of age, leading to the initiation of intravenous immunoglobulin (IVIG) replacement therapy and infection prophylaxis. Genetic analysis detected a likely pathogenic variant on the maternal allele and copy number loss on the paternal allele in TRIM37. Subsequently a cardiac magnetic resonance imaging was conducted revealing signs of pericardial constriction raising concerns for intestinal lymphatic losses. The cessation of IVIG therapy did not coincide with any increase in the rate of infections. The patient exhibited a distinct immunological profile, characterized by hypogammaglobulinemia, impaired antibody responses, and skewed T-cell subsets with an altered CD4+/CD8+ ratio, consistent with previous reports. Normal thymocyte development assessed by artificial thymic organoid platform ruled out an early hematopoietic intrinsic defect of T-cell development.DiscussionThe immunological profile of MUL patients reported so far shares similarities with that described in protein-losing enteropathy secondary to CHF in Fontan circulation and primary intestinal lymphangiectasia. These similarities include hypogammaglobulinemia, significant T-cell deficiency with decreased CD4+ and CD8+ counts, altered CD4+/CD8+ ratios, and significantly modified CD4+ and CD8+ T-cell phenotypes toward effector and terminal differentiated T cells, accompanied by a loss of naïve CD45RA+ T lymphocytes. In MUL, CHF is a cardinal feature, occurring in a significant proportion of patients and influencing prognosis. Signs of CHF or constrictive pericarditis have been evident in the case reported here and in all cases of MUL with documented immune dysfunction reported so far. These observations raise intriguing connections between these conditions. However, further investigation is warranted to in-depth define the immunological defect, providing valuable insights into the pathophysiology and treatment strategies for this condition.
Healing wounds, defeating biofilms: Lactiplantibacillus plantarum in tackling MRSA infections
Authors: Dubey Ashish Kumar , Sharma Mohini , Parul  , Raut Sachin , Gupta Pawan , Khatri Neeraj 
Published: December 2023

IntroductionMethicillin-resistant Staphylococcus aureus (MRSA) infections are well-known hospital-borne infections and are a major contributing factor to global health concerns of antimicrobial resistance due to the formation of biofilms. Probiotics are known to assist in the healing of wounds through immunomodulation and also possess anti-pathogen properties via competitive inhibition. The probiotic bacterium, Lactiplantibacillus plantarum MTCC 2621 and its cell-free supernatant (Lp2621) have previously been reported to have antibacterial, excellent antioxidant, and wound healing activity in in vitro conditions and wounds contaminated with S. aureus in mice.MethodsIn the current study, we evaluated its anti-MRSA, biofilm inhibition and eradication efficacy, immunomodulatory activity in THP-1 cells, and wound healing potential in wounds contaminated with MRSA infection in mice.ResultsIn agar well diffusion assay, Lp2621 showed anti-MRSA activity and revealed dose-dependent inhibition and eradication of biofilm by crystal violet assay as well as by Confocal Scanning Laser Microscopy (CLSM) analysis. Further, Lp2621 showed immunomodulatory activity at varied concentrations as measured by IL-6 and IL-10 gene expression in THP-1 cells. Similar findings were observed in serum samples of mice after treatment of excision wound contaminated with MRSA infection by Lp2621 gel, as evident by expression of IL-6 (pro-inflammatory) and IL-10 (anti-inflammatory) cytokines.ConclusionsOverall, our results show that Lp2621 has potent anti-MRSA and antioxidant properties and can prevent and eliminate biofilm formation. It also showed promise when applied to mice with MRSA-infected wounds.
Human motor neurons derived from induced pluripotent stem cells are susceptible to SARS-CoV-2 infection
Authors: Cappelletti Gioia , Colombrita Claudia , Limanaqi Fiona , Invernizzi Sabrina , Garziano Micaela , Vanetti Claudia , Moscheni Claudia , Santangelo Serena , Zecchini Silvia , Trabattoni Daria , Silani Vincenzo , Clerici Mario , Ratti Antonia , Biasin Mara 
Published: December 2023

IntroductionCOVID-19 typically causes Q7 respiratory disorders, but a high proportion of patients also reports neurological and neuromuscular symptoms during and after SARSCoV-2 infection. Despite a number of studies documenting SARS-CoV-2 infection of various neuronal cell populations, the impact of SARS-CoV-2 exposure on motor neuronal cells specifically has not been investigated so far.MethodsThus, by using human iPSC-derived motor neurons (iPSC-MNs) we assessed: (i) the expression of SARS-CoV-2 main receptors; (ii) iPSC-MN infectability by SARS-CoV-2; and (iii) the effect of SARS-CoV-2 exposure on iPSC-MN transcriptome.ResultsGene expression profiling and immunofluorescence (IF) analysis of the main host cell receptors recognized by SARS-CoV-2 revealed that all of them are expressed in iPSC-MNs, with CD147 and NRP1 being the most represented ones. By analyzing SARS-CoV-2 N1 and N2 gene expression over time, we observed that human iPSC-MNs were productively infected by SARS-CoV-2 in the absence of cytopathic effect. Supernatants collected from SARS-CoV-2-infected iPSC-MNs were able to re-infect VeroE6 cells. Image analyses of SARS-CoV-2 nucleocapsid proteins by IF confirmed iPSC-MN infectability. Furthermore, SARS-CoV-2 infection in iPSCMNs significantly altered the expression of genes (IL-6, ANG, S1PR1, BCL2, BAX, Casp8, HLA-A, ERAP1, CD147, MX1) associated with cell survival and metabolism, as well as antiviral and inflammatory response.Discussion:These results suggest for the very first time that SARS-CoV-2 can productively infect human iPSC-derived MNs probably by binding CD147 and NRP1 receptors. Such information will be important to unveil the biological bases of neuromuscular disorders characterizing SARS-CoV-2 infection and the so called long-COVID symptoms.
Functional force stimulation alters motor neuron discharge patterns
Authors: Xu Yaodan , Du Zuyu , Cheng Anyi , Lin Runwei , Wang Kaichen , Jin Yibin , Dai Chenyun , Xu Lin 
Published: December 2023

IntroductionBeneficial effects have been observed for mechanical vibration stimulation (MVS), which are mainly attributed to tonic vibration reflex (TVR). TVR is reported to elicit synchronized motor unit activation during locally applied vibration. Similar effects are also observed in a novel vibration system referred to as functional force stimulation (FFS). However, the manifestation of TVR in FFS is doubted due to the use of global electromyography (EMG) features in previous analysis. Our study aims to investigate the effects of FFS on motor unit discharge patterns of the human biceps brachii by analyzing the motor unit spike trains decoded from the high-density surface EMG.MethodsEighteen healthy subjects volunteered in FFS training with different amplitudes and frequencies. One hundred and twenty-eight channel surface EMG was recorded from the biceps brachii and then decoded after motion-artifact removal. The discharge timings were extracted and the coherence between different motor unit spike trains was calculated to quantify synchronized activation.Results and discussionSignificant synchronization within the vibration cycle and/or its integer multiples is observed for all FFS trials, which increases with increased FFS amplitude. Our results reveal the basic physiological mechanism involved in FFS, providing a theoretical foundation for analyzing and introducing FFS into clinical rehabilitation programs.
A controlled lumbar puncture procedure improves the safety of lumbar puncture
Authors: Li Chun , Li Miaomiao , Wang Yixian , Li Shaoyi , Cong Lin , Ma Weining 
Published: December 2023

BackgroundIn order to improve the safety of lumbar puncture (LP), we designed a new type of LP needle, that is, an integrated and controlled LP needle, which can actively and accurately control the flow rate and retention of cerebrospinal fluid (CSF) during puncture, so as to achieve a controlled LP procedure.ObjectiveTo evaluate whether a controlled LP procedure can improve the comfort of LP and reduce the risk of complications associated with LP.MethodsPatients requiring LP (n = 63) were pierced with an integrated and controlled LP needle or a conventional LP needle. The differences in vital signs, symptom score, comfort, operation time, CSF loss, CSF pressure fluctuation and back pain before and after puncture were analyzed.ResultsAn integrated and controlled LP needle (n = 35) significantly improved patients’ headache symptoms before and after puncture. In addition, a controlled LP procedure significantly reduced the amount of unnecessary CSF loss (p &lt; 0.001), shortened the time of puncture (p &lt; 0.001), improved patient comfort (p = 0.001) and reduced the incidence of back pain (p &lt; 0.001). For patients with high intracranial pressure (HICP), the fluctuations in pressure of the CSF were also reduced while obtaining similar amounts of CSF (p = 0.009).ConclusionA controlled LP procedure avoids unnecessary CSF loss, prevents rapid fluctuations in CSF pressure in patients with HICP, and reduces the risks associated with LP.
Acute consumption of a branched chain amino acid and vitamin B-6 containing sports drink does not improve multiple sprint exercise performance, but increases post-exercise blood glucose
Authors: Farra Saro D. 
Published: December 2023

PurposeThe aim of this study was to investigate the ergogenicity of BioSteel High Performance Sports Drink (B-HPSD), a commercially available branched chain amino acid (BCAA) and vitamin B-6 (VitB-6) supplement, on multiple sprint exercise (MSE).MethodsEleven experienced cyclists completed two MSE trials in counterbalanced order, after ingesting either B-HPSD (2,256 mg of BCAA, 300 mcg of VitB-6) or placebo (PLA). The MSE protocol consisted of five maximal effort 1 km sprints on a cycle ergometer separated by 2 min of active recovery. Power output (PO) was continuously measured throughout the cycling protocol. Heart rate (HR) and ratings of perceived exertion (RPE) were monitored following each sprint. Capillary blood samples were collected and analyzed for lactate and glucose before and 2 min post-trial. Cognitive function was assessed before and 15 min after the exercise protocol.ResultsThe PO maintained during each 1 km sprint decreased throughout the protocol (p &lt; 0.05), but the change in PO was similar between conditions. Post-exercise blood glucose was elevated after consuming B-HPSD but not PLA (p &lt; 0.05). Blood lactate (p &lt; 0.05), HR (p &lt; 0.05) and RPE (p &lt; 0.05) increased throughout the trials, however no differences were observed between conditions. Cognitive performance improved after exercise (p &lt; 0.05), but the change was similar between conditions.ConclusionThese results demonstrate that acute B-HPSD consumption does not have an ergogenic effect on MSE performance. However, ingestion of B-HPSD increased post-exercise blood glucose concentration when compared to PLA.
Inverse association between isoflavones and prediabetes risk: evidence from NHANES 2007–2010 and 2017–2018
Authors: Zhou Yanjun , Qin Shaolei , Zhu Yan , Xu Peng , Gu Ke 
Published: December 2023

IntroductionPrediabetes is a metabolic condition characterized by blood glucose levels that are higher than normal but do not meet the threshold for a diabetes diagnosis. Individuals with prediabetes are at an increased risk of developing type 2 diabetes and associated complications. However, limited epidemiological studies have investigated the association between flavonoids from plant-based diets and the risk of prediabetes, and the existing evidence from these studies is inconsistent.MethodsTherefore, we utilized data from 19,021 participants (mean age: 32.03 years) in the National Health and Nutrition Examination Survey (NHANES) conducted during 2007–2010 and 2017–2018 to investigate the potential association between dietary flavonoid intake and prediabetes risk by weighted logistic regression analysis. Furthermore, the data from 3,706 participants (mean age: 35.98 years) from NHANES 2007–2010 were used to assess the correlation between concentrations of isoflavones and their metabolites in urine and prediabetes risk by weighted logistic regression analysis.ResultsOur findings revealed an inverse association between the intake of glycitein (OR: 0.88; 95% CI: 0.82–0.96; p = 0.003), genistein (OR: 0.98; 95% CI: 0.97–0.99; p = 0.004), daidzein (OR: 0.98; 95% CI: 0.96–0.99; p = 0.009), and total isoflavones (OR: 0.99; 95% CI: 0.98–1.00; p = 0.005) with the risk of prediabetes. Moreover, we observed an inverse association between the concentration of daidzein in urine (OR: 0.84; 95% CI: 0.73–0.96; p = 0.012) and the concentration of genistein in urine (OR:0.83; 95% CI: 0.75–0.93; p = 0.003) with the risk of prediabetes using weighted logistic regression.ConclusionIn conclusion, our findings suggest a potential protective effect of isoflavones against the development of prediabetes.
Associations of oxidative balance score with total abdominal fat mass and visceral adipose tissue mass percentages among young and middle-aged adults: findings from NHANES 2011–2018
Authors: Wang Kai , Deng Minggang , Wu Jinyi , Luo Lingli , Chen Rui , Liu Fang , Nie Jiaqi , Tao Fengxi , Li Qingwen , Luo Xin , Xia Fang 
Published: December 2023

ObjectiveThis study aimed to explore the association of the oxidative balance score (OBS) with total abdominal fat mass (TAFM) and visceral adipose tissue mass (VATM) percentages among young and middle-aged U.S. adults.MethodsYoung and middle-aged adults in the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2018 were included. Analysis of variance and Rao-Scott adjusted chi-square tests were used to compare the characteristics across quartiles of OBS. Univariate and multivariate weighted logistic regression models were employed to explore the relationship between OBS and the risks of high TAFM or high VATM percentage in the general population and subgroups, while the interaction effects were tested with a likelihood test. Weighted restricted cubic spline analyses were utilized to assess the non-linear association of OBS with TAFM and VATM percentages.ResultsThe final sample included 8,734 young and middle-aged non-institutionalized U.S. adults representing 134.7 million adults. Compared with adults in the first quartile of OBS, those with higher OBS were less likely to have a high TAFM percentage; the ORs and 95% CI for adults in the second, third, and highest quartiles of OBS were 0.70 (0.53–0.94), 0.49 (0.36–0.60), and 0.25 (0.18–0.36), respectively. Similar trends were observed in the association between OBS and VATM percentages. Moreover, similar effects were confirmed in the sensitivity analyses and subgroup analyses according to demographic characteristics. Regarding the OBS subclass, higher dietary OBS and lifestyle OBS were also correlated with decreased ORs of high TAFM and VATM percentages.ConclusionThis study strongly suggests that higher OBS, as well as higher dietary OBS and lifestyle OBS, are significantly correlated with lower risks of abdominal obesity and visceral fat accumulation. The findings highlight the importance of an antioxidant-rich diet and maintaining a healthy lifestyle in reducing the risks.