bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prefoldins are novel regulators of molecular mechanism associated with unfolded protein response in artemisinin resistant P. falciparum malaria
Authors: Singh Shailja , Shoaib Rumaisha , Parveen Nidha , Kumar Vikash , Behl Ankita , Garg Swati , Chaudhary Preeti , Balaya Rex Devasahayam Arokia , Saini Monika , Maurya Preeti , Gupta Nutan , Kumari Jyoti , Jain Ravi , Biswas Shreeja , Pandey Kailash , Abid Mohammad 
Published: November 2023

Abstract
Emerging Artemisinin (ART) resistance in Plasmodium demands novel drugs that can target artemisinin resistance mechanism to manage resistant parasites. ART resistance is attributed by mutations in the Plasmodium falciparum (Pf) Kelch-13 (PfK13) gene, however a study showed that artemisinin resistance is linked with up-regulated expression of unfolded protein response pathways involving Prefoldins (PFD) of malaria parasite. Here, we functionally characterized all Pf Prefoldin subunits, the causative links responsible for providing artemisinin resistance. PfPFD-6 interacts with PfK13 and this phenotype was confirmed in yeast orthologous system to show that Prefoldin decrease the sensitivity of artemisinin in mutant strains. Expression of Prefoldin subunits was upregulated in artemisinin resistant line Pfk13R539T, underscoring their significance in providing artemisinin resistance. PfPFD1-6 localize in the cytosol, and these subunits interact in an orchestrated manner (-PFD3-PFD2-PFD1-PFD5-PFD6-PFD4-) to form a jelly-fish like complex. We identified an FDA approved drug ‘Biperiden’ that restricts the formation of Prefoldin complex and inhibits its interaction with key parasite proteins, MSP-1 and α-tubulin-I. Moreover, Biperiden treatment inhibits the parasite growth in Pf3D7 artimisinin sensitive and resistant line. Overall, our study provides novel virtues towards understanding the role of PfPFDs in artemisinin resistance mechanism, and opens new avenues for the management of resistant parasite.
Ideological Ambiguity and Political Spectrum
Authors: Galindo-Silva Hector 
Published: November 2023

Abstract
This study examines the relationship between ambiguity and the ideological positioning of political parties across the political spectrum. We identify a strong non-monotonic (inverted U-shaped) relationship between party ideology and ambiguity within a sample of 202 European political parties. This pattern is observed across all ideological dimensions covered in the data. To explain this pattern, we argue that centrist parties are perceived as less risky by voters compared to extremist parties, giving them an advantage in employing ambiguity to attract more voters at a lower cost. We support our explanation with additional evidence from electoral outcomes and economic indicators in the respective party countries.
JEL classification: D72.
Active control of bulk photovoltaic effect in CuInP2S6 via flexoelectric engineering
Authors: Li Jiangyu , Yu Junxi , Huang Boyuan , Yang Songjie , Zhang Yuan , Bai Yinxin , Ming Wenjie , Liu Wenyuan , Wang Junling , Li Changjian , Wang Qingyuan 
Published: November 2023

Abstract
Bulk photovoltaic effect (BPVE) offers an interesting approach to generate a steady photocurrent in a single-phase material under homogeneous illumination, and it has been extensively investigated in ferroelectrics exhibiting spontaneous polarization that breaks inversion symmetry. Flexoelectricity breaks inversion symmetry via strain gradient in the otherwise nonpolar materials, enabling manipulation of ferroelectric order in the absence of electric field. Combining these two effects together, we demonstrate active mechanical control of BPVE in suspended 2-dimensional CuInP2S6 (CIPS) that is ferroelectric yet sensitive to electric field, which enables practical photodetection devices with order of magnitude enhancement in performance. It is found that the suspended CIPS exhibits 20-fold increase in photocurrent, which can be continuously modulated by either mechanical force or light polarization angle. The flexoelectrically engineered photodetection device, activated by air pressure and without any optimization, possesses a responsivity of (2.45 x 10-2) A/W and a detectivity of (1.73 x 1011) Jones, which are superior to ferroelectric-based photodetection and comparable to the commercial Si photodiode.
Testis-specific gene C7orf61 is involved in mouse sperm–egg fusion
Authors: Wu Yong , Zhang Zeng , He Chihua , Deng Qiong 
Published: November 2023

Abstract
This study was aimed to investigate the expression of chromosome 7 open reading frame 61 (C7orf61) in the testis and determine its role in spermatogenesis. Reverse transcription–quantitative polymerase chain reaction, Western blot and immunofluorescence were performed to evaluate the expression characteristics of C7orf61 in mice and humans. In vitro fertilization assay was used to determine the role of the C7ORF61 protein in sperm-egg fusion. The results demonstrated that C7orf61 was a testis-specific gene; the C7ofr61 mRNA expression level sharply increased in the fourth postnatal week and gradually increased until the adult stage. The C7ORF61 protein was located throughout the subacrosomal area and close to the nucleus in both mouse and human sperm. The incubation with the C7ORF61 antibody significantly decreased the fertilization rate of mouse eggs. The present findings suggested that the C7ORF61 protein might be involved in sperm–egg fusion, and could serve as a useful target for contraceptives. However, further research is still needed to know the detailed molecular mechanism of its role.
Lenvatinib targets STAT-1 to enhance the M1 polarization of TAMs in hepatocellular carcinoma progression
Authors: Sun Peng , Li Zhenfeng , Yan Zaojun , Wang Zhaofeng , Zheng Peng , Wang Mingliang , Chang Xu , Liu Zihao , Zhang Jianxin , Wu Huiyong , Shao Wenbo , Xue Dewen , Yu Jinming 
Published: November 2023

Abstract
Lenvatinib, a multi-target kinase inhibitor, has been proven to be effective in the treatment of advanced hepatocellular carcinoma. It has been previously demonstrated that tumor associated macrophages (TAMs) in tumor tissues could promote HCC growth, invasion and metastasis. Furthermore, the lenvatinib has certain immunomodulatory activity in the treatment of HCC. However, the role of lenvatinib to the macrophages polarization in the HCC treatment has not been fully explored. In this article, we used a variety of experimental methods both in vitro and in vivo, to investigate the effect of lenvatinib on tumor-associated macrophages in HCC progression. The results showed for the first time that the lenvatinib could alter the macrophages polarization status both in human and mice. Meanwhile, Macrophages which treated with lenvatinib in vitro displayed the enhanced M1 activity and suppressed the proliferation, invasion, migration of liver cancer cells. Furthermore, in the progression of M1 polarization induced by lenvatinib, STAT-1 was the main target transcription factor and STAT-1 activity inhibition could reverse the effect caused by lenvatinib. Altogether, the present study provided a theoretical basis for the immunomodulatory function of lenvatinib in the treatment of HCC.
The refinement of drug safety governance based on contradiction matrix
Authors: yao ye , Yang Fan , Zhang WenXue 
Published: November 2023

Abstract
To construct the contradiction matrix of the refinement of drug safety governance is to provide innovative ideas to solve the difficult problem of drug safety governance. And from this to promote the refinement of drug safety governance and ensure people's safety. The contradictory matrix in TRIZ theory is used to provide innovative ideas. Firstly, To sort out the problems of multiple subjects in drug safety governance based on problem orientation. Secondly, problem parameterization, that is, drug safety governance issues are transformed into the corresponding management parameters, and forming 18 parameters of drug safety governance refinement. Thirdly, The contradiction matrix of the refinement of drug safety management was constructed by combining the principles of 40 inventions. Finally, The practical application value of contradiction parameters and contradiction matrix constructed by case demonstration analysis. The contradiction matrix can be applied to the field of drug safety governance. The constructed parameters of the refinement of drug safety governance can better represent the problem attributes in drug safety governance. The contradiction matrix can provide decision-making aid and innovative ideas for solving problems in drug safety governance.
Assessing the causal relationship between mitochondria-associated proteins and chronic liver diseases：a comprehensive Mendelian randomisation study
Authors: guo ziwei , Ye Wangyu , Wang Jiuchong , Lv Wenliang 
Published: November 2023

Abstract
Mitochondria-related proteins (MRPs) and chronic liver diseases have been linked in various studies, although their causal relationship has not been elucidated. In this study, we investigated the causal associations between MRPs and non-alcoholic fatty liver disease (NALFD), liver cirrhosis and hepatocellular carcinoma (HCC) by two-sample bidirectional Mendelian randomisation(MR) analysis.The random-effect Inverse variance weighted (IVW) is the primary analysis for causality analysis while MR-Egger and Weighted Median (WM) as complementary analyses. Cochran Q test, MR-Egger intercept test, MR-PRESSO and leave-one-out analysis were used for sensitivity analyses. In addition, we performed bonferroni correction,multivariable MR analysis(MVMR),reverse causality detection and protein–protein interaction(PPI) network to enrich the results of this study.After rigorous genetic variant selection, IVW, sensitivity analysis, 3 genetically determined MRPs were significantly associated with NAFLD [MRPL33 (OR: 1.06, 95% CI: 1.00-1.11, p = 0.0284), MRPL34 (OR: 0.88, 95% CI: 0.78–0.98, p = 0.0294) and FARS2 (OR : 0.90, 95% CI: 0.84–0.97, p = 0.0120)], 2 MRPs were significantly associated with liver cirrhosis[MICU1 (OR: 1.11, 95% CI: 1.00-1.22, p = 0.0337) and NUDT8 (OR: 1.16, 95% CI: 1.03–1.30, p = 0.0096)], and 4 MRPs were significantly correlated with HCC [MRPL32 (OR: 0.62, 95% CI: 0.39–0.99, p = 0. 0492), MRPL33 (OR:1.29, 95% CI: 1.07–1.55, p = 0.0063), SCO1 (OR:0.56, 95% CI. 0.38–0.83, p = 0.0036) and SIRT5 (OR:0.71, 95% CI: 0.53–0.96, p = 0.0283)].Our findings provide a new perspective on the exploration of the underlying mechanisms of chronic liver diseases. However, further studies are still needed to explore the mechanisms of possible potential causal associations between MRPs and chronic liver diseases.
MRI-Based Microthrombi Detection in Stroke with Polydopamine Iron Oxide
Authors: Jacqmarcq Charlène , Picot Audrey , Flon Jules , Rubio Marina , Lebrun Florent , de Lizarrondo Sara Martinez , Naveau Mikael , Malzert-Fréon Aurélie , Bernay Benoît , Goux Didier , gauberti Maxime , Vivien Denis , Bonnard Thomas 
Published: November 2023

Abstract

The primary goal of treatments for acute ischemic stroke (AIS) is to restore cerebral blood flow in occluded vessels through thrombolysis, either in conjunction with thrombectomy or as a standalone procedure. However, even after successful recanalization, downstream microcirculation may still be obstructed by microvascular thrombosis leading to compromised brain reperfusion and cognitive decline. Regrettably, identifying these microthrombi through non-invasive methods remains challenging. For this specific purpose, we developed the PHySIOMIC (Polydopamine Hybridized Self-assembled Iron Oxide Mussel Inspired Clusters), an innovative MRI-based contrast agent which unmasks microthrombi in vivo. In a mouse model of thromboembolic ischemic stroke, our findings demonstrate that PHySIOMIC generate a distinct hypointense signal on T2*-weighted MRI in the presence of microthrombi, as confirmed by histological analysis. The extent of hypointense signal at the onset of stroke displayed a correlation with the lesion sizes observed 24 hours post-stroke. Mice treated with thrombolytic agents exhibited a disappearance of the hypointense signal, validating effective thrombolysis of microthrombi. Our microfluidic studies revealed the role of fibrinogen present in the protein corona composition for the targeting of PHySIOMIC to microthrombi. Furthermore, we established the degradation patterns of these particles within the liver and spleen over time. The PHySIOMIC particles offer an innovative and valuable tool for non-invasive in vivo diagnosis and monitoring of microthrombi, using MRI during AIS.
Accessibility to Palliative Care Services in Colombia: An Analysis of Geographic Disparities
Authors: Sánchez-Cárdenas Miguel Antonio , León Marta Ximena , Rodríguez-Campos Luisa Fernanda , Escobar Lina María Vargas , Cabezas Laura , Tamayo-Díaz Juan Pablo , Piñeros Angela Cañon , Manosalva Nidia Mantilla , Fuentes-Bermudez Genny Paola 
Published: November 2023

Abstract
Objectives
Due to the increase in the prevalence of non-communicable diseases and the Colombian demographic transition, the necessity of palliative care has arisen. This study used accessibility and coverage indicators to measure the geographic barriers to palliative care.
Methods
Population-based observational study focused on urban areas and adult population from Colombia, which uses three measurements of geographic accessibility to services: a) density of palliative care services per 100,000 inhabitants, b) analysis of geographic distribution by territorial nodes of the country, and c) spatial analysis of palliative care services using Voronoi diagrams. ArcGIS Pro software was used to map services’ locations and identify geographic disparities.
Results
A total of 504 palliative care services were identified, of which 77% were primary health care services. The density of palliative care services in Colombia is 1.8 primary care services per 100,000 inhabitants and 0.4 specialized services per 100,000 inhabitants. The average palliative care coverage is 41%, two regions of the country have a coverage below 30%. Twenty-eight percent of the services provide care for a population greater than 50,000 inhabitants within their coverage area, exceeding the acceptable limit by international standards.
Conclusions
Palliative care services are concentrated in three main regions (Bogotá D.C., the Center, and the Caribbean) and are limited in the Orinoquia and Amazonia nodes. Density of specialized palliative care services is extremely low and there are regions without palliative services for adults with palliative needs.
A numerical model for vertical two-dimensional non-hydrostatic free surface flows
Authors: En-nefkhaoui Hakim 
Published: November 2023

Abstract
In this paper we describe the development of a two-dimensional vertical non-hydrostaticnumerical model for simulating free surface flows. The model is basedon the solution of the two-dimensional incompressible Navier-Stokes equations inthe sigma-coordinate system. The governing equations are discretized using the finite difference approximation on non-staggered grid and solved based on the algorithm of a projection method, which consists of combining the momentum and continuity equations to establish a Poisson-type equation for the pressure. To predict the moving free surface elevations, the depth-integrated continuity equation is used and solved explicitly by the total variation diminishing Lax-Wendroff scheme. To circumvent the limitations of overly restrictive time steps due to a high aspect ratio between horizontal and vertical spatial discretization. We adopt a strategy based on a split between the advection-diffusion part and the projection part. The advection-diffusion is also split so that it is treated implicitly in the vertical direction and explicitly in the horizontal direction. This discretization technique has enabled us to group the resulting finite difference equations with the appropriate boundary conditions in the form of linear tridiagonal systems with the intermediate velocities as unknowns, which can be efficiently inverted using the Thomas algorithm. The model has been applied to simulate five benchmark-tests of surface wave motions. Comparison between numerical results, analytical solutions and experimental data demonstrates asatisfactory performance.
MSC Classification: 65M06, 65N06, 76D05, 76M20, 76D33
Accurate and Flexible Single Cell to Spatial Transcriptome Mapping with Celloc
Authors: Zhou Yuan , Yin Wang , Wu Xiaobin , Chen Linxi , Wan You 
Published: November 2023

Abstract
Accurate mapping between single-cell RNA sequencing (scRNA-seq) and low-resolution spatial transcriptomics (ST) data compensates for both the limited spatial resolution of ST spots and the inability of scRNA-seq to preserve spatial information. Here, we developed Celloc, a deep learning non-convex optimization-based method for flexible single-cell-to-spot mapping, which enables either dissecting cell composition of each spot (regular mapping) or predicting spatial location of every cell in scRNA-seq data (greedy mapping). We benchmarked Celloc on simulated ST data where Celloc outperformed state-of-the-art methods in accuracy and robustness. Evaluations on real datasets suggested that Celloc could reconstruct the spatial pattern of cells in breast cancer, reveal spatial subclonal heterogeneity of ductal carcinoma in situ, infer spatial tumor-immune microenvironment, and signify spatial expression patterns in myocardial infarction. Together, the results suggest that Celloc can accurately reconstruct cellular spatial structures with various cell types across different histological regions.
Surface Plasmon Based Optical Data storage Devices using Graphene Nanoholes
Authors: A SILPA P 
Published: November 2023

Abstract
We present a high-capacity optical data storage device using nanohole engraved graphene film structure. Nanoholes will work as plasmonic structure such that normally incident radiation can be coupled into surface plasmons without the use of prism-coupling based total internal reflection, as in the classical Kretschmann configuration. Simulations based on Finite Differential Time Domain (FDTD) were carried out to numerically calculate the reflectance - from different type of nano-hole structures - as a function of the localized refractive index of the media around the SPR (Surface plasmon resoanace) holes. Our calculations indicate substantially higher differential reflectance signals, on localized change of refractive index in the narrow hole plasmonic gratings, as compared to those obtained from conventional SPR-based sensing system. In this work, the simulations of the structure were done using Lumerical simulations software. Finally graphene filim –nanohole based surface resonance structures was fabricated using chemical vapour deposition and laser ablation techniques. The viability of the structure towards data storage is verified using Raman spectroscopy and UV spectroscopy
Affect Valuations Predict Emotional Response to Future Exam Success and Failure
Authors: Yoon Sunkyung , Rottenberg Jonathan 
Published: November 2023

Abstract

Does what a person desires to feel (affect valuation) predict their future affective reaction to salient life events? We tested this idea in the context of an exam, a salient achievement-oriented event for college students. One to two weeks prior to taking an exam, 180 university students rated their ideal affect, depression symptom severities, and provided affective forecasts for how they would react to exam failure and success. On the day when students received their exam scores, they rated their actual emotional response to the exam outcomes. Higher levels of ideal positive affect predicted greater positive affective responses to exam success. This prediction held even after controlling for affective forecasting, depression levels, and the exam score itself. Higher levels of ideal negative affect predicted greater negative affective reactions to exam failure, but did not survive parallel statistical controls. Results suggest the possibility that ideal affect performs motivational functions, particularly for positive affective states.
Exosomal miR‑301a‑3p of airway epithelial cells regulates macrophage polarization and promotes lung injury via the GATA1 pathway in acute respiratory distress syndrome
Authors: Lu Xiaoxiao , Ma Wentao , Gao Jing , Liu Qiuhong , Zhao Shilong , Li Jing , Hu Chunling , Yu Xiao , Yao Mengying 
Published: November 2023

Abstract
Objectives: Acute respiratory distress syndrome (ARDS) is associated with high morbidity and mortality rates, and macrophage polarization is critical for its pathogenesis. Exosomes are crucial inflammation mediators; however, airway epithelial cell-derived exosome functions and their mechanisms remain unclear.
Methods: We investigated effects of airway epithelial cell-derived exosomes on lipopolysaccharide (LPS)-induced ARDS in mice. Exosomes isolated from bronchial alveolar lavage fluid of phosphate-buffered saline or LPS-treated mice were injected to C57BL/6 wild type mice intratracheally; macrophage polarization, cytokine secretion, and cell apoptosis were examined. In an in vitro co-culture system, human macrophage precursor (THP-1) was co-cultured with these exosomes.
Results: LPS-induced exosomes promoted M1 macrophage polarization, cytokine secretion, and apoptosis in vivo and in vitro co-culture models. Bioinformatic analysis indicated that miR-301a-3p-mediated LPS-Exosomes (LPS-Exo) functioned via targeting GATA1 downstream pathway in macrophages. Administering miR-301a-3p mimic significantly aggravated LPS-Exo-induced M1 macrophage polarization, inflammatory response, and lung injury, which was partially reversed by miR-301a-3p inhibitor. The miR-301a-3p mediated LPS-Exo function via upregulating the GATA1/NF‑κB and downregulating GATA1/Akt pathways in macrophages.
Conclusions: Exosomal miR-301a-3p derived from airway epithelial cells aggravates ARDS development via inducing M1 macrophage polarization and promoting lung injury via activating the GATA1 pathway.
Migrating Foreign Bodies of Penis: A Case Report
Authors: Yang Bo , Liu Xiaoyun , Ke Ying , Qiu Aixin , Wen Lijie , Xv Xiaolong , Zhang Yue 
Published: November 2023

Abstract

Background Only a few cases have been reported about active foreign body implantation in the cavernous body of the penis, in addition to therapeutic penile prosthesis.Case Presentation: A 47-year-old man inserted two needles from the glans into the bilateral penile sponge body. Subsequently, two needles moved along the penile cavernous body into the pelvic cavity. Attempts to remove the needles through the penis were unsuccessful. Finally, after more than a month, the displaced needles were removed in stages from the buttocks.Conclusion To our knowledge, this is the first case of walking foreign bodies of penis in China. In this condition, it is imperative to engage the expertise of experienced andrologists to minimize the risk of excessive manipulation, thereby ensuring that the needle does not inadvertently penetrate deep into the penile tissue. Clinicians should be aware of the possible psychological issues in patients and prioritize protecting their mental well-being along with the safety of medical workers.