bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coconut (Cocos nucifera L.) and Carob (Ceratonia siliqua L.) Flours Dietary Fibers Differentially Impact Fecal Microbiota Composition and Metabolic Outputs In Vitro
Authors: Arioglu‐Tuncil Seda , Deemer Dane , Lindemann Stephen R. , Tunçil Yunus E. 
Published: January 2025

ABSTRACTAlternative flours can reveal beneficial health effects. The aim of this study was to evaluate and compare the effects of dietary fibers (DFs) of coconut and carob flours on colonic microbiota compositions and function. Coconut flour DFs were found to be dominated by mannose‐containing polysaccharides by gas chromatography (GC)/MS and spectrophotometer, whereas glucose and uronic acid were the main monosaccharide moieties in carob flour DFs. In vitro fecal fermentation analysis revealed that coconut flour DFs result in the generation of microbial butyrate as much as inulin does, which is known to be a butyrogenic prebiotic, but at a slower rate. Supportingly, coconut flour DFs promoted butyrate‐producing bacteria including Roseburia and Coprococcus, whereas carob flour DFs stimulated Prevotella‐related OTUs. In addition, higher microbial diversity was achieved at the end of the fermentation of coconut flour DFs by the fecal microbiota. This study clearly shows that alternative flours have distinct functionalities in terms of colonic microbiota composition and function, and coconut flour could be used as an alternative flour for the development of functional food products targeting colonic health.
Removal of turfgrass for water supply resilience
Authors: Dukes Michael D. 
Published: January 2025

AbstractLandscape plant palettes are changing due to water supply pressure. Turfgrass area is being replaced with alternative ground covers, other non‐irrigated plants or hardscapes. Mechanisms range from incentive programs sometimes called “cash for grass” to outright bans on the use of turfgrass in “nonfunctional” areas or areas otherwise considered unnecessary. Despite evidence that shifts in landscape plants such as turf removal can save water, questions remain about the long‐term impact of such programs as well as the high cost. This shift in plant palettes will need to be accompanied by development of new groundcovers. In addition, there is opportunity for turfgrass breeding and development for cultivars that require no irrigation in humid climates.
Demographic, anthropometric and intrasubject variations affect platelet‐rich plasma formulation
Authors: García‐Bordes Luis , Álvarez‐Díaz Pedro , Alentorn‐Geli Eduard , Ferré‐Aniorte Alfred , Laiz‐Boada Patricia , Seijas‐Vázquez Roberto , Cugat‐Bertomeu Ramon 
Published: January 2025

AbstractPurposeThe purpose of this study is to describe the inter‐ and intra‐individual differences in the platelet concentration between blood and platelet‐rich plasma (PRP) preparation, assess intersubject differences considering demographic and anthropometric variables, describe PRP code distribution and analyse intrasubject variability.MethodsA retrospective analysis was conducted using a single‐centre patient database from November 2021 to November 2023. It included patients with musculoskeletal pathologies treated with PRP injections. Primary variables were demographic characteristics (sex, age, body mass index [BMI]) and platelet concentrations in blood and PRP during treatments. Secondary analysis focused on PRP code frequency distribution and intrasubject variability according to different coding systems.ResultsHere, 686 patients met the inclusion criteria. PRP exhibited significantly higher platelet concentrations compared to blood (378.01 ± 136.25 × 103 platelets/µL vs. 221.97 ± 58.21 × 103 platelets/µL, p < 0.001). Younger patients had higher platelet concentrations in both blood (p = 0.004) and PRP (p = 0.003), whereas female patients showed higher platelet concentrations only in blood (p < 0.001). The platelet concentration ratio was higher in males (p < 0.001) and those with higher BMI (p = 0.023). Significant differences were found between the existing and modified PRP coding systems (p < 0.001). Intrasubject variability was higher in PRP than in blood (coefficient of variance: 21.32 ± 17.36 in blood vs. 27.85 ± 19.10 in PRP, p < 0.001).ConclusionAge, gender, BMI and intrasubject variations significantly affect PRP formulation, emphasizing the importance of addressing these variables for a more predictable, personalized and effective therapeutic approach.Level of EvidenceThis is a retrospective study. Level IV.
The use of four K‐wires does not lead to a reduction of the MPTA in the context of a one‐dimensional tibial deflection correction of patients with ACL re‐rupture and pathologically increased tibial slope
Authors: Arras Christian , Korthaus Alexander , Frings Jannik , Berninger Markus T. , Fahlbusch Hendrik , Frosch Karl‐Heinz , Drenck Tobias , Akoto Ralph , Krause Matthias 
Published: January 2025

AbstractPurposeAnterior tibial closing wedge osteotomy (ATCWO) has been shown to significantly reduce failure rates of revision anterior cruciate ligament (ACL) reconstructions in patients with a posterior tibial slope (PTS) ≥12°. Recent findings suggest a slight but significant reduction of the medial proximal tibial angle (MPTA) resulting in a varus knee where the sagittal osteotomy plane is based on a total of two guide wires defining the osteotomy wedge without respecting the frontal plane. We hypothesize that the placement of a total of four guide wires intraoperatively can reduce the influence on the MPTA.MethodsThis study retrospectively reports on a two‐centre series of 42 ATCWOs for PTS correction between January 2022 and December 2023 at two clinical centres. A total of four guide wires were placed based on a true lateral intraoperative view of the tibia, with two positioned each at the cranial and at the caudal pole of the osteotomy wedge, serving as guides for the saw to create the osteotomy, with careful attention to ensuring that the proximal and distal K‐Wires were placed parallel to each other. A retrospective analysis was conducted by examining true lateral and anteroposterior radiographs to identify changes in sagittal and coronal plane alignment.ResultsThe study included 19 women and 23 men, with a mean age of 29.7 ± 8.6 years with first‐time ACL revision surgery and a minimum PTS of ≥12°. PTS decreased significantly from 14.5 ± 2.8° preoperatively to 8.2 ± 1.9° post‐operatively (p < 0.001). The aMPTA demonstrated no significant difference between preoperative (mean aMPTA: 86.9 ± 2.1°) and post‐operative (mean aMPTA: 86.6 ± 1.9°) measurements (p > 0.05).ConclusionWith our technique of placing four guide wires to achieve precise guidance during the insertion of the osteotomy wedge, there is no substantial impact on the aMPTA during slope correction.Level of EvidenceLevel IV.
Clinical outcomes and biomechanics in bicruciate‐retaining total knee arthroplasty
Authors: Kono Kenichi , Kage Tomofumi , Yamazaki Takaharu , Taketomi Shuji , Yamagami Ryota , Inui Hiroshi , Tomita Tetsuya , Tanaka Sakae 
Published: January 2025

AbstractPurposeTo clarify the influence of biomechanics on post‐operative clinical outcomes in bicruciate‐retaining total knee arthroplasty (BCR‐TKA).MethodsSevere medial osteoarthritis who underwent BCR‐TKA were examined. Each patient was asked to perform a squat (weight‐bearing [WB]) and active assisted knee flexion (non‐WB [NWB]) under single fluoroscopy surveillance. A 2D‐to‐3D registration technique was used. Patients were divided into two groups based on their 1‐year post‐operative patient‐reported outcome measures (PROMs) using hierarchical cluster analysis. The rotational alignment on computed tomography, anterior stability at 30° of knee flexion, axial rotation of the femur relative to the tibial component and anteroposterior translation of the medial and lateral femorotibial contact points were measured.ResultsComponents did not significantly differ between the groups, with 1.6 ± 5.0° and 5.4 ± 4.7° of femoral internal rotation in the low PROM (N = 28) and high PROM (N = 8) groups, respectively. Moreover, anterior stability did not significantly differ (low PROM: 4.9 ± 1.4 mm, high PROM: 5.3 ± 1.0 mm). The knee externally rotated from 0° to 70° and from 50° to 110° of flexion during WB and NWB, respectively. The low‐PROM group exhibited more external rotation across all ranges of motion. Medial contact points moved backwards from 0° to 30° of flexion during WB, forward from 30° to 100° of flexion, and backwards from 100° to 110° of flexion. The low‐PROM group was positioned more forward throughout the full range of motion during WB. Lateral contact points moved backwards at 0−30° of flexion, forward at 70−100° of flexion, and backwards at 100−110° of flexion during WB, while there was backward movement at 50° of flexion during NWB. Both activities exhibited a more posterior position in the low‐PROM group throughout the full range of motion.ConclusionThe femoral component in the low‐PROM group was externally rotated across all ranges of motion, and the lateral contact points were posteriorly located in BCR‐TKA.Level of EvidenceLevel II, prospective cohort study.
Cartilage thickness can be accurately measured intraoperatively in total knee arthroplasty: A step further in calipered kinematic alignment
Authors: Giurazza Giancarlo , Campi Stefano , Hirschmann Michael T. , Franceschetti Edoardo , Tanzilli Andrea , Gregori Pietro , Paciotti Michele , Zampogna Biagio , Papalia Rocco 
Published: January 2025

AbstractIntroductionKinematic alignment (KA) in total knee arthroplasty (TKA) is by definition a pure femoral resurfacing procedure aiming to restore the individual prearthritic anatomy. However, when a 2 mm compensation is systematically used on the worn side, the variability in cartilage thickness in the unworn compartment might alter the accuracy of the technique. This study aimed to validate two intraoperative femoral cartilage thickness measurement techniques by comparing them to the photographic method, which measures cartilage thickness through pixel analysis of bone‐cut images. The study hypothesized that the two intraoperative methods are comparable and similarly accurate within 0.5 mm of the photographic method.MethodsSeventy cartilage thickness measurements from seventy patients with end‐stage knee osteoarthritis were prospectively collected. Two intraoperative techniques were evaluated: the electrocautery tip method (Method A) and the ruler method (Method B), performed before and after distal femoral bone resections, respectively. The postoperative photographic analysis (Method C) served as the reference method. Measurements were rounded to the nearest 0.5 mm for consistency. Data were analyzed using Kruskal–Wallis test, Wilcoxon rank‐sum tests, Spearman's rank correlation, percentage of agreement and intraclass correlation coefficients (ICCs).ResultsNo significant differences were observed between Method A and Method B in measuring femoral cartilage thickness. Agreement with Method C was 100% for Method B and 85% for Method A. In the 15% of discordant cases, Method A overestimated the measurements by one category of 0.5 mm compared to Method C. Correlation coefficients between the methods were high (ρ = 0.88−1.0). Intra‐ and interobserver reliability was high for all methods (ICCs 0.91–0.95).DiscussionBoth intraoperative methods are reliable and comparable to the photographic method when rounded to the closest 0.5 mm, with no significant differences among them. The electrocautery method has the added advantage of measuring cartilage thickness before bone cuts are performed.Level of EvidenceLevel IV.
Comparison of taurolidine with 4% ethylenediaminetetraacetic acid on antimicrobial lock effectiveness: An experimental study
Authors: Visek Jakub , Ryskova Lenka , Cesakova Petra , Stanclova Jana , Vajrychova Marie , Blaha Vladimir 
Published: January 2025

AbstractBackgroundAntimicrobial lock therapy is recommended for preventing and treating catheter‐related bloodstream infections, but different solutions have uncertain efficacy.MethodsTwo locks, 1.35% taurolidine and 4% ethylenediaminetetraacetic acid (EDTA), were tested on Staphylococcus epidermidis, Staphylococcus aureus, methicillin‐resistant S. aureus, Pseudomonas aeruginosa, multidrug‐resistant P. aeruginosa, vancomycin‐resistant Enterococcus faecium, Klebsiella oxytoca (carbapenemase producing), K. pneumoniae (extended‐spectrum β‐lactamase producing), Candida albicans, and Candida glabrata. Broviac catheter segments were incubated with these organisms and then exposed to various lock solutions. Colony‐forming units (CFUs) were counted after 2, 4, and 24 h of incubation.ResultsTaurolidine showed a significant decrease in CFUs after 2 h in S. aureus, S. epidermidis, methicillin‐resistant S. aureus, vancomycin‐resistant E. faecium, P. aeruginosa (both sensitive and multidrug‐resistant strains), K. oxytoca, C. albicans, and C. glabrata. After 4 h, significant reductions were noted in S. aureus, S. epidermidis, methicillin‐resistant S. aureus, P. aeruginosa, multidrug‐resistant P. aeruginosa, K. pneumoniae, K. oxytoca, and C. albicans. Taurolidine was also effective after 24 h, especially against methicillin‐resistant S. aureus and multidrug‐resistant P. aeruginosa. Four percent EDTA acid showed a significant reduction in CFUs after 2 h in S. aureus, vancomycin‐resistant E. faecium, P. aeruginosa, K. oxytoca, C. albicans, and C. glabrata. After 4 h, reductions occurred in P. aeruginosa, multidrug‐resistant P. aeruginosa, K. oxytoca, and C. albicans and after 24 h in methicillin‐resistant S. aureus, P. aeruginosa, and K. oxytoca.ConclusionTaurolidine is more effective than 4% EDTA acid in eradicating Gram‐positive and Gram‐negative microorganisms and fungi.
Return to competition after ACL reconstruction: Factors influencing rates and timing in Swedish football players
Authors: Sandon Alexander , Kvist Joanna , Hedevik Henrik , Forssblad Magnus 
Published: January 2025

AbstractPurposeTo investigate the rate and timing for return to football league games after anterior cruciate ligament reconstruction (ACLR) in Swedish players, examining associations with sex, age, level, graft and additional ACL surgery.MethodData from the Swedish National Knee Registry (SNKLR) and the Swedish Football Association's IT System (FOGIS) were used. The study cohort comprised 971 football players, 64% males, who underwent primary ACLR. Demographics, graft type and surgical information were extracted from the SNKLR and game participation from FOGIS. Follow‐up for return to competition (RTC) was conducted for 36 months, while additional ACLR follow‐up was 3–7 years. Statistical analyses, including Kaplan–Meier survival curves and relative risk calculations, were employed to assess factors influencing RTC rates and timing.ResultsOut of 971 players analyzed, 53% RTC within 3 years with no difference between males and females, at a mean of 15 months (median 14 months) from surgery to the first game. Eleven (2%) players RTC < 6 months from ACLR, 62 (12%) 6–9 months, 125 (24%) 9–12 months and 331 (63%) >12 months. Patellar tendon (PT) grafts demonstrated superior performance, showing quicker returns and higher RTC rates (p = 0.005) compared to hamstring (hazard ratio [HR]: 0.63 [0.48–0.84]) and quadriceps tendon grafts (HR: 0.53 [0.30–0.93]). Players competing in higher divisions pre‐injury experienced significantly swifter and higher RTC rates (p < 0.001). Ninety‐five (10%) had a registered additional ACLR. Players who RTC did not exhibit a significantly higher rate of revision (35 [7%] vs. 25 [5%]). However, those who returned faced a heightened risk of contralateral ACLRs compared to those who did not RTC (32 [6%] vs. 4 [1%] RR 1.72 [1.59–1.96], p < 0.001).ConclusionThe study reveals that 53% of football players RTC after ACLR, predominantly after more than 12 months. The RTC was higher and faster in high‐level players and those receiving a PT graft. The slow RTC may contribute to the relatively low rate of additional ACLRs.Level of EvidenceLevel III.
Quantum Chemical NMR Spectroscopic Structural Analysis in Solution: The Investigation of 3‐Indoleacetic Acid Dimer Formation in Chloroform and DMSO Solution
Authors: Da Silva Haroldo C. , Hernandes Isabel S. , De Almeida Wagner B. 
Published: January 2025

ABSTRACTWe present a DFT‐PCM NMR study of 3‐indoleacetic acid (3‐IAA), used as a working example, including explicit solvent molecules, named PCM‐nCHCl3, PCM‐nDMSO (n = 0, 2, 4, 8, 14, 20, and 25), to investigate the dimer formation in solution. Apart from well‐known cyclic (I) and open (II) acetic acid (AA) dimers, two new structures were located on DFT‐PCM potential energy surface (PES) for 3‐IAA named quasicyclic A (III) and quasicyclic B (IV), the last one having N–H…O hydrogen bond (instead of O–H…O). In addition, four other structures having π–π type interactions named V, VI, VII, and VIII were also obtained completing the sample on the PES. Our theoretical results and experimental 1H NMR data (CDCl3) strongly indicate that 3‐IAA should exist in a quasicyclic form (III) in a chloroform solution different from AA. Solute–solvent interactions play a key role in O–H and N–H chemical shifts. The strong H‐bond formation between the S=O and O–H and N–H groups produces large chemical shift value THAT masquerades the identification of dimer formation in DMSO solution based on 1H NMR chemical shift changes. However, analysis of 13C NMR and relative energy DFT‐PCM‐nDMSO results strongly indicate the presence of parallel ring interacting dimer having OH…benzene ring bond (VI). There can be a competition between solute–solute and solute–solvent interactions, and polar DMSO solvent can break the quasicyclic dimers (III and IV) intermolecular O–H…O and N–H…O bonds yielding two solvated monomeric species hydrogen bonded to O=S(CH3)2 groups, what may take place for other organic molecules in solution. However, it did not happen for the π–π interacting dimers and structure VI survived in DMSO solution.
Inpatient Audiograms: A Mixed Methods Study Examining Clinical Decision Making, Financial Analyses, and Audiologist Perspectives
Authors: Ibrahim Nadine I. , Cherry Megan M. , Keilin Charles A. , Abdulrazzak Obada , Anidi Chioma , Epperson Madison V. , Jones Gerilyn , Banakis Hartl Renee M. 
Published: January 2025

AbstractObjectiveEvaluate inpatient audiometry on clinical decision‐making. Assess stakeholder perspectives on the practice of inpatient audiometry and financial impact.Study DesignThis is a mixed methods study utilizing retrospective chart review, a focus group, and financial analyses.SettingAcademic tertiary referral center.MethodsIncluded subjects were adults (18+) admitted from 2015 to 2022 who received an inpatient audiogram (n = 302). A binary assessment of whether audiograms impacted inpatient clinical management was determined. Financial analyses estimated the cost of audiograms. An audiology focus group was conducted.ResultsThe average age was 51 years. Thirty‐nine percent were female. Seventy percent of inpatient audiograms were associated with Otolaryngology consultation. Inpatient audiograms were performed an average of 3.6 days after request (0‐47 days, 90th percentile 8.2 days). Forty‐nine percent were performed within 1 day. Twenty‐three percent were performed for acute hearing loss. Ototoxic monitoring was the most frequent indication (15%). Sixty‐two percent of audiograms did not impact the initiation or withholding of treatment. Inpatient testing results in a 63% increase in cost over outpatient. Audiologists endorsed challenges with equipment, patient‐level factors, and system‐level challenges.ConclusionInpatient audiometry is resource‐intensive without significant data examining the impact and clinical utility. In most cases, inpatient audiometry is not used in the decision to withhold or deliver treatment and may cost 63% more than outpatient audiograms. While inpatient audiometry has a critical role in appropriate settings, system optimization and guidelines are necessary. Outpatient audiograms may suffice for the majority of otologic consults in combination with a thorough history and physical exam. Additional study across institutions with variable practice would be beneficial to establish broader recommendations.
Understanding and Improving Pediatric Hearing Care Navigation: A Human‐Centered Design Approach
Authors: Rashidi Neema , Lindeborg Michael M. , Stephans Jihyun , Bellfort‐Salinas Silvia , Naugle Kendyl , Wong Michelle A. , Chan Dylan K. 
Published: January 2025

AbstractObjectiveExplore the experiences of stakeholders within hearing care pathways using a human‐centered design process to design a patient navigator (PN) to improve hearing health equity for deaf or hard‐of‐hearing children.Study DesignA qualitative, prospective, observational study utilizing the Empathize, Define, and Ideate phases of Human‐Centered Design.SettingAcademic tertiary children's hospital with interviews over Zoom.MethodsWe interviewed 12 hearing care providers, diverse in role and education across the United States, and 10 parents of deaf or hard‐of‐hearing children, diverse in preferred language and delays in care (mean age at diagnosis of 4.7 months, mean age at intervention of 10.5 months). The main outcomes were themes and subsequent insights that arose from the thematic analysis of interviews, which were used within a design synthesis session.ResultsQualitative analysis revealed 3 themes: Intrinsic Barriers, Extrinsic Barriers, and Areas of Opportunity. Within Intrinsic Barriers, a majority of providers and parents described a grieving process that led to delays. Within Extrinsic Barriers, interviewees expressed challenges navigating multiple deaf or hard‐of‐hearing care pathways. Within Areas of Opportunity, providers and parents agreed on connecting parents of new hearing loss diagnoses with mentor families to improve the care‐seeking experience. Intercoder reliability was achieved (Cohen's κ = 0.73). Findings from the qualitative analysis were incorporated in a synthesis session with diverse stakeholders to design a PN role.ConclusionProviders and parents agree that the deaf or hard‐of‐hearing care pathway is difficult to navigate at baseline with delays in accessing care further exacerbated by barriers intrinsic to the family unit.