bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unlocking cellular plasticity: Enhancing human iPSC reprogramming through bromodomain inhibition and extracellular matrix gene expression regulation
Authors: Yang Jun , Karimi Kinyamu H. , Ward James M. , Scappini Erica , Archer Trevor K. 
Published: October 2023

ABSTRACTThe transformation of fibroblasts into epithelial cells is critical for iPSC reprogramming. In this report, we describe studies with PFI-3, a small molecule inhibitor that specifically targets the bromodomains of SMARCA2/4 and PBRM1 subunit of SWI/SNF complex, as an enhancer of iPSC reprogramming efficiency. Our findings revealed that PFI-3 induces cellular plasticity in multiple human dermal fibroblasts, leading to a mesenchymal-epithelial transition (MET) during iPSC formation. This transition was characterized by the upregulation of E-cadherin expression, a key protein involved in epithelial cell adhesion. Additionally, we identified COL11A1 as a reprogramming barrier and demonstrated COL11A1 knockdown increased reprogramming efficiency. Notably, we found that PFI-3 significantly reduced the expression of numerous extracellular matrix (ECM) genes, particularly those involved in collagen assembly. Our research provides key insights into the early stages of iPSC reprogramming, highlighting the crucial role of ECM changes and cellular plasticity in this process.
Non-verbal skills in auditory word processing: Implications for typical and dyslexic readers
Authors: Jhilal Soufiane , Molinaro Nicola , Klimovich-Gray Anastasia 
Published: October 2023

AbstractDyslexia is characterized by poor phonological skills and atypical neural responses to text and speech stimuli, while non-verbal intelligence remains unaffected. However, the impact of non-verbal intelligence on language-evoked neural responses in dyslexia is underexplored. This study examines non-verbal intelligence’s effects on neural responses to auditory words in typical and dyslexic readers. Participants completed IQ tests, reading and phonological assessments, and underwent magnetoencephalography recordings while listening to words. Event-related brain field responses at different stages of auditory word processing (100ms, 200ms, 400ms) were analyzed in relation to non-verbal IQ scores and reading performance. Higher non-verbal IQ in typical readers yielded earlier right hemisphere perceptual responses (100ms). Dyslexic readers showed no IQ-related latency effects but those with lower non-verbal IQ displayed amplified left hemisphere response amplitudes at 100ms. These findings confirm the link between non-verbal cognitive abilities and early auditory word processing in adults, highlighting distinct effects in dyslexia and controls.
The fatty liver disease-causing protein PNPLA3-I148M alters lipid droplet-Golgi dynamics
Authors: Sherman David J. , Liu Lei , Mamrosh Jennifer L. , Xie Jiansong , Ferbas John , Lomenick Brett , Ladinsky Mark S. , Verma Rati , Rulifson Ingrid C. , Deshaies Raymond J. 
Published: October 2023

AbstractNon-alcoholic fatty liver disease (NAFLD), recently renamed metabolic dysfunction-associated steatotic liver disease (MASLD), is a progressive metabolic disorder that begins with aberrant triglyceride accumulation in the liver and can lead to cirrhosis and cancer. A common variant in the genePNPLA3, encoding the protein PNPLA3-I148M, is the strongest known genetic risk factor for MASLD to date. Despite its discovery twenty years ago, the function of PNPLA3, and now the role of PNPLA3-I148M, remain unclear. In this study, we sought to dissect the biogenesis of PNPLA3 and PNPLA3-I148M and characterize changes induced by endogenous expression of the disease-causing variant. Contrary to bioinformatic predictions and prior studies with overexpressed proteins, we demonstrate here that PNPLA3 and PNPLA3-I148M are not endoplasmic reticulum-resident transmembrane proteins. To identify their intracellular associations, we generated a paired set of isogenic human hepatoma cells expressing PNPLA3 and PNPLA3-I148M at endogenous levels. Both proteins were enriched in lipid droplet, Golgi, and endosomal fractions. Purified PNPLA3 and PNPLA3-I148M proteins associated with phosphoinositides commonly found in these compartments. Despite a similar fractionation pattern as the wild-type variant, PNPLA3-I148M induced morphological changes in the Golgi apparatus, including increased lipid droplet-Golgi contact sites, which were also observed in I148M-expressing primary human patient hepatocytes. In addition to lipid droplet accumulation, PNPLA3-I148M expression caused significant proteomic and transcriptomic changes that resembled all stages of liver disease. Cumulatively, we validate an endogenous human cellular system for investigating PNPLA3-I148M biology and identify the Golgi apparatus as a central hub of PNPLA3-I148M-driven cellular change.Significance StatementFatty liver disease affects nearly a quarter of the world’s population and has both environmental and genetic risk factors. A mutation in the genePNPLA3that converts Ile 148 to Met is the strongest known genetic risk factor for developing fatty liver disease. Using a series of techniques to track endogenous PNPLA3 and PNPLA3-I148M biogenesis and localization, we reveal new insights into how the mutation changes cellular dynamics. Although previous reports focus on its role on lipid droplets, we reveal that PNPLA3-I148M also functions at the Golgi apparatus, an organelle critical for protein transport into and out of the cell and lipid signaling. PNPLA3-I148M causes altered Golgi morphology and drives changes reminiscent of liver disease.
Concentration-effect relationships of plasma caffeine on EEG delta power and cardiac autonomic activity during human sleep
Authors: Baur Diego M. , Dornbierer Dario A. , Landolt Hans-Peter 
Published: October 2023

AbstractAcute caffeine intake affects brain and cardiovascular physiology, yet the concentration-effect relationships on the electroencephalogram (EEG) and cardiac autonomic activity during sleep are poorly understood. To tackle this question, we simultaneously quantified the plasma caffeine concentration with ultra-high-performance liquid chromatography, as well as the EEG, heart rate and high-frequency (0.15-0.4 Hz) spectral power in heart rate variability (HR-HRV), representing parasympathetic activity, with standard polysomnography during undisturbed human sleep. Twenty-one healthy young men ingested in randomized, double-blind, cross-over fashion, 160 mg caffeine or placebo in a delayed, pulsatile-release caffeine formula at their habitual bedtime, and initiated a four-hour sleep opportunity 4.5 hours later. The mean caffeine levels during sleep exhibited high individual variability between 0.2 and 18.4 µmol/l. Across the first two NREM-REM sleep cycles, EEG delta (0.75-2.5 Hz) activity and heart rate were reliably modulated by waking and sleep states. Caffeine dose-dependently reduced delta activity and heart rate, and increased HR-HRV in NREM sleep when compared to placebo. The average reduction in heart rate equaled 3.24 ± 0.77 beats per minute. Non-linear statistical models suggest that caffeine levels above ∼ 7.4 µmol/l decreased EEG delta activity, whereas concentrations above ∼ 4.3 µmol/l and ∼ 4.9 µmol/l, respectively, reduced heart rate and increased HR-HRV. The findings provide quantitative concentration-effect relationships of caffeine, EEG delta power and cardiac autonomic activity and suggest increased parasympathetic activity during sleep after intake of caffeine.
Evaluation of fresh vegetable microbiological contamination in fast-food restaurants
Authors: Saleh Farag Ali , Al-Otaibi Mutlag Mohammad 
Published: October 2023

PurposeFresh vegetables contain advantageous phytochemical components, making them one of the most significant sources of nutrition. The threat of harmful bacteria still exists because these vegetables are not heated in restaurants before being consumed. Therefore, this study aimed to evaluate the microbial quality of fresh vegetables in restaurants of different levels.Design/methodology/approachA total of 499 fresh vegetable samples (from sandwiches and fresh-cut vegetable salads) were collected from 3 different types of food service establishment: 201 from international restaurants (IRs), 210 from national restaurants (NRs), and 88 from cafeterias (CAs). The samples were prepared and inoculated on specific growth media. The aerobic mesophilic bacteria (AMB) Campylobacter spp., Staphylococcus aureus (S. aureus), Enterobacteriaceae, Escherichia coli (E. coli) and yeast and molds were counted, and Listeria monocytogenes, Salmonella spp. and Escherichia coli O157 were detected using specialized medium.FindingsHigh counts of S. aureus, above 3 log cfu/g, suggested that 71.5% of samples collected from NRs and 77.3% from CA were not accepted, whereas 81.6% of samples collected from IRs were accepted. The low population of E. coli, less than 2 log cfu/g, suggested that 99.0, 97 and 92.0 % of samples collected from IRs, NRs and CA, respectively, were accepted. Listeria monocytogenes and Escherichia coli O157 were absent from every sample. One sample was positive for Salmonella spp. in each of the NR and CA sample groups.Originality/valueRIs adhere to health and hygiene standards better than NRs and CAs, according to the findings of vegetable contamination tests.
What drives the effectual entrepreneurship: combinative effects of environmental uncertainty and entrepreneurs’ means
Authors: Bao Shuqin , An Wenwen , Wang Aihuan , Luo Shunjun 
Published: October 2023

Purpose
Effectuation, which articulates the process of entrepreneurial action based on nonpredictive control logic, is receiving extensive scholarly attention. What drives the effectual entrepreneurship is featured with high complexity. However, existing studies ignored the complex driving forces underlying entrepreneurial decision-making. Building on a configurational perspective, the purpose of this study was to examine the combinative effects of environmental uncertainty and entrepreneurs’ means on effectual entrepreneurship.

Design/methodology/approach
Drawing on 54 entrepreneurs who are launching new ventures in China, this study adopts a fuzzy-set Qualitative Comparative Analysis (fsQCA) to investigate two sets of antecedent conditions and how they form different combinations for a highly effectual entrepreneurship.

Findings
Our findings disclose four highly effectual entrepreneurship paths involving novice–specialist effectual entrepreneurship in a highly uncertain environment, socialite–specialist effectual entrepreneurship in a highly uncertain environment, pure-specialist effectual entrepreneurship and resourceful effectual entrepreneurship, and one path of barefoot noneffectual entrepreneurship in a highly uncertain environment, which reveals the complex nature of environmental uncertainty and entrepreneurs’ means in driving entrepreneurs to adopt effectuation.

Originality/value
Our study makes the following contributions. First, by taking a configurational perspective, we are able to obtain an elaborate view of the combined effects of environmental uncertainty and entrepreneurs’ means on effectual entrepreneurship. Second, we expand prior thinking on the relationship between environmental uncertainty and effectuation. Third, our study offers a more delicate understanding of entrepreneurs’ means in driving effectuation by splitting means into three separate factors.
An artificial bee colony based-hyper heuristic algorithm with local search for the assembly line balancing problems
Authors: Deliktaş Derya , Aydin Dogan 
Published: October 2023

PurposeAssembly lines are widely employed in manufacturing processes to produce final products in a flow efficiently. The simple assembly line balancing problem is a basic version of the general problem and has still attracted the attention of researchers. The type-I simple assembly line balancing problems (SALBP-I) aim to minimise the number of workstations on an assembly line by keeping the cycle time constant.Design/methodology/approachThis paper focuses on solving multi-objective SALBP-I problems by utilising an artificial bee colony based-hyper heuristic (ABC-HH) algorithm. The algorithm optimises the efficiency and idleness percentage of the assembly line and concurrently minimises the number of workstations. The proposed ABC-HH algorithm is improved by adding new modifications to each phase of the artificial bee colony framework. Parameter control and calibration are also achieved using the irace method. The proposed model has undergone testing on benchmark problems, and the results obtained have been compared with state-of-the-art algorithms.FindingsThe experimental results of the computational study on the benchmark dataset unequivocally establish the superior performance of the ABC-HH algorithm across 61 problem instances, outperforming the state-of-the-art approach.Originality/valueThis research proposes the ABC-HH algorithm with local search to solve the SALBP-I problems more efficiently.
Unstable propagation of hydraulic fractures under varying well spacing and initiation sequence of multiple horizontal wells considering shear stress shadows
Authors: Wang Yongliang , Liu Nana 
Published: October 2023

PurposeMulti-well hydrofracturing is an important technology to create new fractures and expand existing fractures to increase reservoir permeability. The propagation morphology of the fracture network is affected by the disturbance between the fractures initiation sequences and spacings between adjacent wells. However, it remains unclear how well spacing and initiation sequences lead to fracture propagation, deflection and connection.Design/methodology/approachIn this study, the thermal-hydro-mechanical coupling effect in the hydrofracturing process was considered, to establish a finite element-discrete element model of multistage hydrofracturing in a horizontal well. Using typical cases, the unstable propagation of hydraulic fractures in multiple horizontal wells was investigated under varying well spacing and initiation sequences. Combined with the shear stress shadow caused by in situ stress disturbed by fracture tip propagation, the quantitative indexes of fracture propagation such as length, volume, displacement vector, deflection and unstable propagation behavior of the hydrofracturing fracture network were analyzed.FindingsThe results show that the shear stress disturbance caused by multiple hydraulic fractures is a significant factor in multi-well hydrofracturing. Reducing the spacing between multiple wells increases the stress shadow area and aggravates the mutual disturbance and deflection between the fractures. The quantitative analysis results show that a decrease of well spacing reduces the total length of hydraulic fractures but increases the total volume of the fracture; compared with sequential and simultaneous fracturing, alternate fracturing can effectively reduce stress shadow area, alleviate fracture disturbance and generate larger fracture propagation length and volume.Originality/valueThe numerical models and results of the unstable propagation and stress evolution of the hydraulic fracture network under thermal-hydro-mechanical coupling obtained in this study can provide useful guidance for the evaluation and design of rock mass fracture networks in deep unconventional oil and gas reservoirs.
Advantages and challenges of extra care housing in the UK for people living with dementia: a scoping review
Authors: Atkinson Teresa June , Oatley Rebecca , Evans Simon 
Published: October 2023

Purpose
The purpose of this paper is to report on a scoping review of the advantages and challenges of extra care housing (ECH) provision in the UK for people living with dementia. Access to suitable housing is a fundamental right for people living with dementia and can enable people to live as well as possible (Twyford and Porteus, 2021). Understanding the advantages and disadvantages of different models of housing with care has been identified as a research priority by people living with dementia (Barrett et al., 2016) but “there is no current consensus on the best model of specialist housing for people with dementia” (Twyford and Porteus, 2021, p. 29).

Design/methodology/approach
This scoping review identifies the advantages and disadvantages of living in ECH for people with dementia. It is the preliminary stage of a study that seeks to develop knowledge about different models of ECH for people living with dementia (Atkinson et al., 2021).

Findings
Advantages include the promotion of independence, flexible staffing, safety and security, social inclusion, physical design and integrated service provision. Disadvantages include barriers to entry, tensions between independence and support, managing advanced dementia, resourcing flexible care, managing social exclusion, loneliness and stigma and a disabling environment.

Research limitations/implications
The scoping review reinforces the need for further research into different models of ECH provision in the UK for people living with dementia. The review provides insight that is of benefit to all stakeholders involved in ECH and contributes to the development of evidence-based provision called for in the recent All Party Parliamentary Group inquiry (Twyford and Porteus, 2021).

Originality/value
This scoping review summarises the current position for people living with dementia in ECH.
An early childhood teacher experience in lesson study: the case of Sara
Authors: Ramos Fonseca Maria Gorete , da Ponte João Pedro 
Published: October 2023

PurposeThis research aims to understand the learning with regard to didactic knowledge of an early childhood teacher who participated in a Lesson Study (LS) on the topic of patterns, as well as her perspective about LS as a teacher education process.Design/methodology/approachThis is a qualitative case study of an early childhood teacher who participated in a LS alongside three other colleagues and a facilitator. Data collection was made by participant observation with elaboration of a research journal, audio recording of sessions and a collective interview. Data analysis was made by content analysis based on the conceptual framework.FindingsThis case study shows that early childhood teachers may become aware of the importance of working with their children mathematical ideas. It also shows that they may develop their didactic knowledge in several dimensions such as school mathematics, curriculum, children and their learning, and teaching practice and that they may appreciate LS as a means of professional development.Originality/valueThis study shows the potential of LS for the professional development of early childhood teachers, a scarcely researched area. It also shows that their learning may include dimensions related to specific curricular areas such as mathematics.
Why do and why don't consumers use fashion rental services? A consumption value perspective
Authors: Noe Heeju , Hyun Jonghan 
Published: October 2023

PurposeThe study utilized the consumption value theory to explore the motivational factors that define and differentiate the users and nonusers of fashion rental servicesDesign/methodology/approachA focus group was conducted to generate an initial list of measurement items. These items were refined through a pretest and then used in a self-administered online questionnaire to collect data from a total of 300 users and 300 nonusers. The collected data were analyzed using factor analysis to identify the factors that define users and nonusers. A MANOVA was then conducted to explore the differences in the identified factors between users and nonusers.FindingsUsing factor analysis, nine factors were extracted across the five consumption values (functional, social, emotional, conditional and epistemic). MANOVA revealed a significant difference between users and nonusers across all factors. Further analyses suggested that the most differentiating factors are two emotional value factors and one social value factor.OriginalityDespite existing studies of fashion rental services, it is debatable whether the phenomenon is fully understood since previous studies primarily focus on consumers who engage in fashion renting services – there is a lack of focus on nonusers. This study provides unique contributions by exploring the phenomenon from both the user's and the nonuser's perspective.
Developing internal marketing strategies for measuring and managing employee-based brand equity
Authors: Baca Granit , Reshidi Nail 
Published: October 2023

Purpose
The purpose of this study is to propose a conceptual framework for measuring and managing employee-based brand equity. Drawing upon existing research, the authors argue that internal brand management should be approached from professional and socio-emotional perspectives.

Design/methodology/approach
The study establishes a comprehensive conceptual framework by thoroughly reviewing existing literature on employee-based brand equity and internal marketing. It builds upon existing research while adding unique insights to deepen the understanding of the subject.

Findings
The proposed conceptual framework highlights the importance of both professional and socio-emotional factors in building and managing employee-based brand equity. The framework emphasises the role of employees’ emotions, values and relationships in shaping their brand-related behaviours and their functional role in delivering brand promises.

Research limitations/implications
This paper offers a theoretical foundation for future research in internal branding. However, the framework is not empirically tested, and further research is needed to validate and refine the framework. Organisations can develop more effective internal branding strategies that enhance brand equity by recognising the importance of both professional and socio-emotional factors in shaping employees’ brand-related behaviours.

Practical implications
Organisations can develop more effective internal branding strategies that enhance brand equity by recognising the importance of both professional and socio-emotional factors in shaping employees’ brand-related behaviours.

Originality/value
This study presents a novel approach to internal brand management, introducing a unique, dual-perspective model. This enriches the current body of literature and provides fresh insights for academics and practitioners in the field of marketing and brand management.