bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improvement of cell growth in green algaeChlamydomonas reinhardtiithrough co-cultivation with yeastSaccharomyces cerevisiae
Authors: Karitani Yukino , Yamada Ryosuke , Matsumoto Takuya , Ogino Hiroyasu 
Published: September 2023

AbstractBiological fixation methods have attracted considerable attention because they can be applied for the fixation of dilute CO2in the atmosphere. Co-cultivation of certain microalgae with heterotrophic microorganisms can increase the growth potential of microalgae under dilute CO2conditions. The objective of this study was to determine the culture conditions under which the growth potential of green algaeChlamydomonas reinhardtiiis enhanced by co-culturing with the yeastSaccharomyces cerevisiae, and to identify the cause of the enhanced growth potential using transcriptome analysis. WhenC. reinhardtiiandS. cerevisiaewere co-cultured with an initial green algae to yeast inoculum ratio of 1:3, the cell concentration ofC. reinhardtiireached 133 × 105cells/mL on day 18 of culture, which was 1.5 times higher than that of the monoculture. Transcriptome analysis revealed that the expression levels of 363 green algae and 815 yeast genes were altered through co-cultivation. These include genes responsible for ammonium transport and CO2enrichment mechanism in green algae and the genes responsible for glycolysis and stress responses in yeast. In conclusion, we identified the culture condition suitable for the co-cultivation ofC. reinhardtiiandS. cerevisiae. In addition, we discuss the cause of the increased growth potential ofC. reinhardtiibased on transcriptome analysis data. Although further studies are needed to elucidate the full impact of microbial interactions inC. reinhardtiiandS. cerevisiaeco-cultures, the findings of this study represent an important first step toward achieving this goal.
Test-retest reliability of MEG functional brain connectivity related to language processing
Authors: Ala-Salomäki Heidi , van Vliet Marijn , Kujala Jan , Roine Timo , Liljeström Mia , Salmelin Riitta 
Published: September 2023

ABSTRACTThe number of studies examining changes in functional connectivity of the human brain is increasing rapidly. In this magnetoencephalography (MEG) study, we examined the reliability of dynamic connectivity related to language processing in a picture naming test-retest paradigm, using data collected from the same participants on two separate days. We determined the connections that were reliable across both days and also examined the behavioral, functional, and structural properties underlying this reliability. A particularly salient finding among a rich set of results was a reliable pattern of beta connectivity increase in the left motor and frontal regions (0–400 ms and 400–800 ms after stimulus onset) and gamma connectivity decrease in the bilateral motor regions (800–1200 ms) which we suggest to represent the motor preparation of speech production. Furthermore, the reliable connections tended to be more frequently associated with the behavioral performance than the non-reliable ones. Finally, the reliable connections were also linked to stronger functional connectivity, as well as to stronger structural connectivity and shorter structural path length, as determined through diffusion MRI (magnetic resonance imaging). Overall, this study defines reliable language-related functional connectivity and introduces practices that may increase reliability.AUTHOR SUMMARYResearch applying connectivity metrics in neuroimaging has increased rapidly during recent years. Hence, the focus has also been to define the best methods for increasing the reliability of connectivity estimation. This study determined reliable functional connectivity from MEG data related to language processing. Moreover, we defined what makes a connection reliable by studying the behavioral, functional, and structural properties underlying the reliable connections.
Is there a spinal tap responder in progressive supranuclear palsy?: The first prospective study
Authors: Ohara Masahiro , Hattori Takaaki , Chen Qingmeng , Shimano Kaoru , Hirata Kosei , Matsui Mie , Yokota Takanori 
Published: September 2023

AbstractObjectiveProgressive supranuclear palsy (PSP) is a progressive neurodegenerative disease, and sometimes shows idiopathic normal pressure hydrocephalus (iNPH)-like presentations. We aimed to evaluate spinal tap responsiveness in patients with PSP, including the effect of sham spinal tap.MethodsEleven patients with PSP, ten with probable/definite iNPH, and eight control patients were prospectively enrolled. All participants underwent sham spinal tap and spinal tap procedures. Gait was evaluated using wearable inertial sensors. We defined “tap responders” as individuals with a 10% or more improvement from baseline in any of the gait parameters (timed up-and-go test total time, stride length, and velocity during straight walking under single-task and cognitive dual-task conditions). We compared the ratio of tap responders in patients with PSP to patients with iNPH and controls.ResultsThe ratio of tap responders in patients with PSP was significantly higher than that in control patients, but not different from patients with iNPH. The ratio of sham tap responders was significantly higher in PSP patients than that in control patients, but not that in patients with iNPH. Notably, one patient with PSP responded to the spinal tap beyond the effect of sham spinal tap, and even to the shunt operation.InterpretationThis is the first prospective study to demonstrate tap and shunt responsiveness in patients with PSP while highlighting the placebo effects of the spinal tap in patients with PSP and iNPH. Our findings suggest that some PSP patients have impaired cerebrospinal fluid circulation, contributing to a distinct component of the clinical spectrum.
Exploration of the potential of high resolution and contrast 7 Tesla MR brain imaging in neonates
Authors: Bridgen Philippa , Tomi-Tricott Raphael , Uus Alena , Cromb Daniel , Quirke Megan , Almalbis Jennifer , Bonse Beya , De la Fuente Botella Miguel , Maggioni Alessandra , Di Cio Pierluigi , Cawley Paul , Casella Chiara , Dokumaci Ayse Sila , Thomson Alice R , Willers Moore Jucha , Bridglal Devi , Saravia Joao , Finck Thomas , Price Anthony N , Pickles Elisabeth , Cordero-Grande Lucilio , Egloff Alexia , O’Muircheartaigh Jonathan , Counsell Serena J , Giles Sharon L , Deprez Maria , De Vita Enrico , Rutherford Mary A , Edwards A David , Hajnal Joseph V , Malik Shaihan J , Arichi Tomoki 
Published: October 2023

ABSTRACTBACKGROUNDUltra-high field MR imaging offers marked gains in signal-to-noise ratio, spatial resolution, and contrast which translate to improved sensitivity for pathology and anatomy. These benefits are particularly relevant for the neonatal brain, as it is rapidly developing and sensitive to injury. However, experience of imaging neonates at 7T has been limited due to regulatory, safety, and practical considerations.PURPOSETo establish a program for safely acquiring high resolution and contrast brain images from neonates on a 7T system.STUDY TYPEProspective case series.POPULATIONImages were safely acquired from 35 neonates on 44 occasions (median age 39+6 postmenstrual weeks, range 33+4 to 52+6; median body weight 2.93kg, range 1.57to 5.3kg) over a median of 49 mins 30 sec.FIELD STRENGTH/SEQUENCE7T, acquired sequences included T2 weighted (TSE), Actual Flip angle Imaging, functional MRI (BOLD EPI), susceptibility weighted imaging, and MR spectroscopy (STEAM).ASSESSMENTPeripheral body temperature, physiological measures (heart rate, oxygen saturations). Review of acquired images by Neonatal Neuroradiologists for visual identification of anatomy and pathology, and by radiographer and researcher for assessment of image quality.STATISTICAL TESTSTwo tailed paired t-test, P<0.05 was considered statistically significant.RESULTSThere was no significant difference between temperature before and after scanning (p=0.76). Image quality assessment compared favourably to state-of-the-art 3T acquisitions. Anatomical imaging demonstrated excellent sensitivity to structures which are typically hard to visualise at lower field strengths including the hippocampus, cerebellum, and vasculature. The potential of 7T imaging is highlighted using contrast mechanisms which are enhanced at ultra-high field including susceptibility weighted imaging, functional MRI, and MR spectroscopy.DATA CONCLUSIONWe demonstrate safety and feasibility of imaging vulnerable neonates at ultra-high field. Our preliminary imaging suggests ultra-high field has untapped potential to provide important new insights into brain development and pathological processes during this critical phase of early life.
Brain oscillatory processes related to sequence memory in healthy older adults
Authors: Ehrhardt Nina M. , Flöel Agnes , Li Shu-Chen , Lucchese Guglielmo , Antonenko Daria 
Published: September 2023

AbstractSequence memory is subject to age-related decline, but the underlying processes are not yet fully understood. We analyzed electroencephalography (EEG) in 21 healthy older (60-80 years) and 26 young participants (20-30 years) and compared time-frequency spectra and theta-gamma phase-amplitude-coupling (PAC) during encoding of the order of visually presented items. In older adults, desynchronization in theta (4-8 Hz) and synchronization in gamma (30-45 Hz) power did not distinguish between subsequently correctly and incorrectly remembered trials, while there was a subsequent memory effect for young adults. Theta-gamma PAC was modulated by item position within a sequence for older but not young adults. Specifically, position within a sequence was coded by higher gamma amplitude for successive theta phases for later correctly remembered trials. Thus, deficient differentiation in theta desynchronization and gamma oscillations during sequence encoding in older adults may reflect neurophysiological correlates of age-related memory decline. Furthermore, our results indicate that sequences are coded by theta-gamma PAC in older adults, but that this mechanism might lose precision in aging.
Brain Glutathione and GABA+ levels in autistic children
Authors: Song Yulu , Hupfeld Kathleen E. , Davies-Jenkins Christopher W. , Zöllner Helge J. , Murali-Manohar Saipavitra , Abdul-Nashirudeen Mumuni , Crocetti Deana , Yedavalli Vivek , Oeltzschner Georg , Alessi Natalie , Batschelett Mitchell A. , Puts Nicolaas A.J. , Mostofsky Stewart H. , Edden Richard A.E. 
Published: September 2023

AbstractAutism spectrum disorder (ASD) is a neurodevelopmental condition characterized by social communication challenges and repetitive behaviors. Altered neurometabolite levels, including glutathione (GSH) and gamma-aminobutyric acid (GABA), have been proposed as potential contributors to the biology underlying ASD. This study investigated whether cerebral GSH or GABA levels differ between a large cohort of children aged 8-12 years with ASD (n=52) and typically developing children (TDC, n=49). A comprehensive analysis of GSH and GABA levels in multiple brain regions, including the primary motor cortex (SM1), thalamus (Thal), medial prefrontal cortex (mPFC), and supplementary motor area (SMA), was conducted using single-voxel HERMES MR spectroscopy at 3T. The results revealed no significant differences in cerebral GSH or GABA levels between the ASD and TDC groups across all examined regions. These findings suggest that the concentrations of GSH (an important antioxidant and neuromodulator) and GABA (a major inhibitory neurotransmitter) do not exhibit marked alterations in children with ASD compared to TDC. A statistically significant positive correlation was observed between GABA levels in the SM1 and Thal regions with ADHD inattention scores. No significant correlation was found between metabolite levels and hyper/impulsive scores of ADHD, measures of core ASD symptoms (ADOS-2, SRS-P) or adaptive behavior (ABAS-2). While both GSH and GABA have been implicated in various neurological disorders, the current study provides valuable insights into the specific context of ASD and highlights the need for further research to explore other neurochemical alterations that may contribute to the pathophysiology of this complex disorder.Lay summaryAutism spectrum disorder (ASD) is a neurodevelopmental condition characterized by social communication challenges and repetitive behaviors. Altered glutathione (GSH, an important antioxidant and neuromodulator) and gamma-aminobutyric acid (GABA, a major inhibitory neurotransmitter) levels have been proposed as potential contributors to the biology underlying ASD. Here, we used advanced edited Magnetic Resonance Spectroscopy (MRS) to measure levels of these low-concentration metabolites in four brain regions of a pediatric cohort. Contrary to our hypothesis, no significant difference was found between ASD and control subjects in either GSH or GABA levels in any brain region.
The spread ofAedes albopictusin the islands of São Tomé and Príncipe
Authors: Rader Jonathan A. , Serrato-Capuchina Antonio , Anspach Tayte , Matute Daniel R. 
Published: September 2023

ABSTRACTThe mosquitoAedes albopictusis a vector species of Dengue, yellow fever, and Zika among other diseases. The species originated in Southeast Asia and has spread widely and rapidly in the last century. The species has been reported in localities from the Gulf of Guinea since the early 2000s, but systematic sampling has been scant. We sampledAe. albopictusbetween 2013 and 2023 across the altitudinal gradient in São Tomé and found that the species was present in all sampled years at altitudes up to 680 meters. We also find some evidence of increases in proportional representation compared toAe. aegyptiover time. We report the presence of the species in Príncipe for the first time, suggesting that the range ofAe. albopictusis larger than previously thought. Finally, we use bioclimatic niche modeling to infer the potential range ofAe. albopictusand infer that the species has the potential to spread across a large portion of São Tomé and Príncipe. Our results suggest thatAe. albopictushas established itself as a resident species of the islands of the Gulf of Guinea and should be incorporated into the list of potential vectors that need to be surveyed and controlled.
CO2Response Screen in GrassBrachypodiumReveals Key Role of a MAP-Kinase in CO2-Triggered Stomatal Closure
Authors: Lopez Bryn N. K. , Ceciliato Paulo H. O. , Rangel Felipe J. , Salem Evana A. , Kernig Klara , Takahashi Yohei , Chow Kelly , Zhang Li , Sidhom Morgana A. , Seitz Christian G. , Sibout Richard , Laudencia-Chingcuanco Debbie L. , Woods Daniel P. , McCammon J. Andrew , Vogel John P. , Schroeder Julian I. 
Published: September 2023

AbstractPlants respond to increased CO2concentrations through rapid stomatal closure which can contribute to increased water use efficiency. Grasses display faster stomatal responses than eudicots due to dumbbell-shaped guard cells flanked by subsidiary cells working in opposition. However, forward genetic screening for stomatal CO2signal transduction mutants in grasses has not been reported. The grass modelBrachypodium distachyonis closely related to agronomically important cereal crops, sharing largely collinear genomes. To gain insights into CO2control mechanisms of stomatal movements in grasses, we developed a forward genetics screen with an EMS-mutagenizedBrachypodium distachyonM5 generation population using infrared imaging to identify plants with altered canopy leaf temperature at elevated CO2. Among isolated mutants, a “chill1” mutant exhibited cooler leaf temperatures than wildtype Bd21-3 parent control plants after exposure to increased [CO2].chill1plants showed strongly impaired high CO2-induced stomatal closure, despite retaining a robust abscisic acid-induced stomatal closing response. Through bulked segregant whole-genome-sequencing analyses followed by analyses of further backcrossed F4 generation plants and generation and characterization of CRISPR-cas9 mutants,chill1was mapped to a protein kinase,BdMPK5. Thechill1mutation impaired BdMPK5 protein-mediated CO2/HCO3-sensingin vitro. Furthermore, AlphaFold2-directed structural modeling suggests that the identified BdMPK5-D90Nchill1mutant residue is located at the interface with the HT1 Raf-like kinase. BdMPK5 is a key signaling component involved in CO2-induced stomatal movements, potentially functioning as a component of the CO2sensor in grasses.
Autosomal suppression of sex-ratio meiotic drive influences the dynamics of X and Y chromosome coevolution
Authors: Gupta Anjali , Unckless Robert L. 
Published: September 2023

AbstractSex-ratio meiotic drivers are selfish genes or gene complexes that bias the transmission of sex chromosomes resulting in skewed sex ratios. Existing theoretical models have suggested the maintenance of a four-chromosome equilibrium (with driving and standard X and suppressing and susceptible Y) in a cyclic dynamic, studies of natural populations have failed to capture this pattern. Although there are several plausible explanations for this lack of cycling, interference from autosomal suppressors has not been studied using a theoretical population genetic framework even though autosomal suppressors and Y-linked suppressors coexist in natural populations of some species. In this study, we use a simulation-based approach to investigate the influence of autosomal suppressors on the cycling of sex chromosomes. Our findings demonstrate that the presence of an autosomal suppressor can hinder the invasion of a Y-linked suppressor under some parameter space, thereby impeding the cyclic dynamics, or even the invasion of Y-linked suppression. Even when a Y-linked suppressor invades, the presence of an autosomal suppressor can prevent cycling. Our study demonstrates the potential role of autosomal suppressors in preventing sex chromosome cycling and provides insights into the conditions and consequences of maintaining both Y-linked and autosomal suppressors.
B-cell specific expression of the murineMyd88L252Pmutation correlates with the establishment of an immunosuppressive microenvironnement in Waldenström macroglobulinemia lymphoplasmacytic lymphoma
Authors: Lemasson Quentin , Téteau Ophélie , Chabaud Mina , Jeanson Laurie , Feuillard Jean , Vincent-Fabert Christelle 
Published: September 2023

AbstractWaldenström macroglobulinemia is a rare and indolent lymphoproliferative disorder genetically characterized by the presence of the L265P mutation in theMYD88gene in nearly each case. Despite its slow progression, Waldenström macroglobulinemia remain incurable due to the lack of specific treatments. The importance of the tumour microenvironment was well documented since the last decade especially concerning the study of solid tumours, but the failures of the immune microenvironment are also experiencing growing interest for B cell lymphomas. In this study, we investigated the implication of some dysregulations of the immune microenvironment in Waldenström macroglobulinemia through ourMyd88L252Pmutated transgenic model, which may explain its progression. In essence, we highlighted the existence of multiple immune escape mechanisms that lead to T-cell exhaustion and participate to Waldenström disease progression. This work in animals opens up new prospects for the development of new therapeutic combinations in WM targeting reactivation of the immune system.
INAEME: Integral Neoantigen Analysis with Entirety of Mutational Events
Authors: Kovacevic Vladimir , Milicevic Ognjen S. , Ilic Raicevic Nevena M. , Kojicic Milica , Mijalkovic Lazic Ana , Skundric Nikola , DiGiovanna Jack 
Published: September 2023

AbstractNeoantigens are peptides on the surface of cancer cells presented to the immune system. Multiple novel therapeutic approaches involve the administration of neoantigens to trigger immunity-induced tumor regression. Identification of neoantigens includes a personalized approach consisting of detailed analyses of the sequenced tumor tissue and its comparison with wild type to identify somatic mutations. Alternated peptides are translated from nucleotides around somatic mutations and their binding affinity and immunogenicity need to be further evaluated. Still, the entire bioinformatics analysis is very complex, and accurate prediction of the neoantigen candidates represents a true challenge. Here, we present the novel, integral bioinformatic analysis workflow for neoantigen discovery, denoted INAEME (Integral Neoantigen Analysis with Entirety of Mutational Events). The workflow performs integral processing of an individual’s DNA tumor-normal and RNA tumor raw reads to output prioritized neoantigen candidates. Our evaluation analysis includes a wide scope of mutational events so far not considered in the existing solutions, including phasing of variants, influence of both somatic and germline variants, positions of all transcripts, neighboring variants, and frameshifts. The influence of each mutational event on the accuracy of predicted neoantigen candidates is tested across 300 TCGA samples from multiple cancer types. The obtained results have demonstrated the significance of considering the entirety of mutational events to obtain an accurate set of strong neoantigen candidates for cancer immunotherapy targets or vaccines. The adaption of the described methods in the bioinformatics analysis minimizes the existence of false positives which are only later discovered in a laboratory environment using expensive methods such as mass spectrometry or microscopy.
Thermal suppression of gametogenesis can explain historical collapses in larval recruitment
Authors: Okamoto Daniel K. , Spindel Nathan B. , Mustermann Maya J. , Karelitz Sam , Collicutt Brenna , Gimenez Iria , Rolheiser Kate , Cronmiller Evan , Foss Megan , Mahara Natalie , Swezey Dan , Ferraro Rachele , Rogers-Bennett Laura , Schroeter Stephen 
Published: September 2023

AbstractProjections for population viability under climate change are often made using estimates of thermal lethal thresholds. These estimates vary across life history stages and can be valuable for explaining or forecasting shifts in population viability. However, sublethal temperatures can also depress vital rates and shape fluctuations in the reproductive viability of populations. For example, heatwaves may suppress reproduction, leading to recruitment failure before lethal temperatures are reached. Despite a growing awareness of this issue, tying sublethal effects to observed recruitment failure remains a challenge especially in marine environments. We experimentally show that sublethal suppression of female gametogenesis by marine heatwaves can partially explain historical collapses in urchin recruitment. These responses differ by sex but are similar between animals from warmer or cooler regions of their range. Overall, we show sublethal thermal sensitivities of reproduction can narrow the thermal envelope for population viability compared to predictions from lethal limits.
WITHDRAWN: HER2 Cellular Localization, Cell-Cell Contacts, and Cell Density Regulate Cancer Cell Plasticity in HER2+ Breast Cancer
Authors: Sameri Saba , Das Durdam , Shahrivari Shabnam , Milosevic Vladan , Sarhadi Shamim , Koerkel-Qu Huiqin , Fisch Carolina , Kokal Miriam , Schletter Michael , Hahn Paul S. , Seider Lisa , Schübel Marisa , Michaelis Stefanie , Wegener Joachim , Weber Florian , Hoffmann Martin , Werno Christian , Weidele Kathrin , Bruckmann Astrid , Östman Arne , Tamm Ernst R. , Li Yuting , Klein Christoph A. , Hosseini Hedayatollah 
Published: September 2023

Withdrawal statementThe authors have withdrawn this manuscript due to a conflict of interest among the authors. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.