bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous and rapid colorimetric detection of distinct miRNAs using Split-LAMP
Authors: Chua Yi Jing , Sim Steven Poh Chuen , Shridharan Medha , Seow Yiqi 
Published: November 2023

Introduction: Aberrant microRNA (miRNA) expressions are often discovered in many life threatening diseases such as cancer. In particular, recent studies show combinations of miRNA levels have greater diagnostic accuracy as opposed to single miRNA levels. For point-of-care applications, rapid and sensitive isothermal amplification with loop-mediated isothermal amplification (LAMP) has gained significant interest.Method: We developed a cost-effective point-of-care testing (POCT) device for multiple miRNAs that can integrate miRNA signals into a single output.Results and Discussion: We demonstrate that the loop primers for LAMP can be broken and be used for miRNA detection. This split-LAMP approach provides a logic AND-gate output for two distinct miRNA inputs. We then show that this is potentially useable in point-of-care testing using pH-sensitive dye to give a rapid, colorimetric endpoint readout within 30 min. This novel logic gate approach can potentially be extended to multiple miRNAs such that there can be a powerful diagnostic concept for multiple short RNAs in a point-of-care rapid test.
Impact of enzyme replacement therapy and migalastat on left atrial strain and cardiomyopathy in patients with Fabry disease
Authors: Pogoda Christian , Brand Stefan-Martin , Duning Thomas , Schmidt-Pogoda Antje , Sindermann Jürgen , Lenders Malte , Brand Eva 
Published: November 2023

AimsCardiomyopathy in Fabry disease (FD) is a major determinant of morbidity and mortality. This study investigates the effects of FD-specific treatment using enzyme replacement therapy (ERT) and chaperone therapy on left atrial (LA) function using two-dimensional speckle tracking echocardiography (2DSTE).Methods and resultsIn this prospective observational single-center study, 20 FD patients [10 (50%) females] treated with migalastat, 48 FD patients [24 (50%) females] treated with ERT (agalsidase-alfa and agalsidase-beta), and 30 untreated FD patients (all females) as controls were analyzed. The mean follow-up time ranged from 26 to 81 months. 2DSTE was performed for left ventricle strain, right ventricle strain, and LA strain (LAS). FD-specific treated patients presented with increased left ventricular mass index (LVMi) and higher frequency of left ventricular hypertrophy at baseline, whereas untreated control patients showed normal baseline values. FD-specific treated (including migalastat and ERT) patients showed stabilization of LAS over time (p &gt; 0.05). LVMi was also stable in treated FD patients during observation (p &gt; 0.05).ConclusionIn patients with FD, treated with either ERT or chaperone therapy, LAS values measured by echocardiographic speckle tracking were stable over time, pointing toward disease stabilization.
Four different gene-related cone–rod dystrophy: clinical and genetic findings in six Chinese families with diverse modes of inheritance
Authors: Li Zhen , Cheng Wanyu , Zi Feiyin , Wang Juan , Huang Xiaoyu , Sheng Xunlun , Rong Weining 
Published: November 2023

Purpose: To investigate pathogenic variants in six families with cone–rod dystrophy (CORD) presenting various inheritance patterns by using whole-exome sequencing (WES) and analyzing phenotypic features.Methods: A total of six families with CORD were enrolled in Ningxia Eye Hospital for this study. The probands and their family members received comprehensive ophthalmic examinations, and DNA was abstracted from patients and family members. Whole-exome sequencing was performed on probands to screen the causative variants, and all suspected pathogenic variants were determined via Sanger sequencing. Furthermore, co-segregation analysis was performed on available family members. The pathogenicity of novel variants was predicted using in silico analysis and evaluated according to the American College of Medical Genetics and Genomics (ACMG) guidelines.Results: Of the six families, two families were assigned as X-linked recessive (XL), two families were assigned as autosomal recessive (AR), and two families were assigned as autosomal dominant (AD). Pathogenic variants were detected in CACNA1F in two X-linked recessive probands, among which family 1 had a hemizygous frameshift variant c.2201del (p.Val734Glyfs*17) and family 2 had a hemizygous missense variant c.245G&gt;A (p.Arg82Gln). Both probands had high myopia, with fundus tessellation accompanied by abnormalities in the outer structure of the macular area. The homozygous splice variant c.2373 + 5G&gt;T in PROM1 and the homozygous nonsense variant c.604C&gt;T (p.Arg202Ter) in ADAM9 were detected in two autosomal recessive families of the probands. Both probands showed different degrees of atrophy in the macular area, and the lesions showed hypofluorescence changes in autofluorescence. The heterozygous variation in CRX c.682C&gt;T (p.Gln228Ter) was detected in two autosomal dominant families. The onset age of the two probands was late, with better vision and severe macular atrophy. According to ACMG guidelines and the analysis of online in silico tools, all variations were labeled as potentially harmful or pathogenic.Conclusion: Pathogenic variants in CACNA1F, PROM1, ADAM9, and CRX genes were identified in six families affected by the diverse inheritance patterns of CORD. Furthermore, the potential impact of the nonsense-mediated decay (NMD) mechanism on the manifestation of CORD phenotypes was examined and addressed. Simultaneously, the spectrum of pathogenic variants and clinical phenotypes associated with the CORD gene was extended.
Perioperative mortality among geriatric patients in Ethiopia: a prospective cohort study
Authors: Endeshaw Amanuel Sisay , Molla Misganew Terefe , Kumie Fantahun Tarekegn 
Published: November 2023

BackgroundWith the dramatic growth in the aged population observed in developed and developing nations, the older population burdened by unmet demand for surgical treatment has become a significant yet unnoticed public health concern in resource-limited countries. Studies are limited regarding surgical mortality of geriatric patients in Africa. Therefore, this study aims to estimate the incidence and identify predictors of postoperative mortality using prospective data in a low-income country, Ethiopia.Methods and materialsA prospective cohort study was conducted from June 01, 2019, to June 30, 2021, at a tertiary-level hospital in Ethiopia. Perioperative data were collected using an electronic data collection tool. Cox regression analysis was used to identify predictor variables. The association between predictors and postoperative mortality among geriatrics was computed using a hazard ratio (HR) with a 95% confidence interval (CI); p-value &lt;0.05 was a cutoff value to declare statistical significance.ResultsOf eligible 618 patients, 601 were included in the final analysis. The overall incidence of postoperative mortality among geriatrics was 5.16%, with a rate of 1.91 (95% CI: 1.34, 2.72) deaths per 1,000 person-day observation. Age ≥ 80 years (Adjusted hazard ratio (AHR) = 2.59, 95% CI: 1.05, 6.36), ASA physical status III/IV (AHR = 2.40, 95%CI 1.06, 5.43), comorbidity (AHR = 2.53, 95% CI: 1.19, 7.01), and emergency surgery (AHR = 2.92, 95% CI: 1.17, 7.27) were the significant predictors of postoperative mortality among older patients.ConclusionPostoperative mortality among geriatrics was high. Identified predictors were age ≥ 80 years, ASA status III/IV, comorbidity, and emergency surgery. Target-specific interventions should be addressed to improve high surgical mortality in these patients.
Case Report: RNF213 variant and choroidal anastomosis as potential risk factors for early stroke in moyamoya syndrome associated with Down syndrome
Authors: Yamamoto Keisuke , Kaku Yasuyuki , Koga Hiroshi 
Published: November 2023

IntroductionRecent studies have suggested associations between RNF213 variants and the formation of periventricular anastomosis among patients with moyamoya disease, leading to early onset of cerebral hemorrhage and rebleeding.Case descriptionWe report herein the case of a boy with Down syndrome and moyamoya syndrome. Exome sequencing identified a heterozygous RNF213 R4810K variant. After ischemic stroke occurred at 9 years old, indirect surgical revascularization was performed for the left cerebral hemisphere and improved ischemic symptoms and cerebral hypoperfusion, while the left choroidal anastomosis remained. At 13 years old, he presented with left thalamic hemorrhage attributed to the anterior choroidal artery, with rebleeding observed four days after the initial hemorrhage under strict blood pressure control. The patient was discharged without neurological deficits 20 days after the hemorrhagic stroke.ConclusionPresence of an RNF213 variant and choroidal anastomosis may represent risk factors for cerebral hemorrhage in patients with Down syndrome and moyamoya syndrome, as well as in patients with moyamoya disease.
Effects of oral cysteine leukotriene receptor antagonist-montelukast on adenoid lymphoid tissue: a histopathological study under light microscope
Authors: Wang Zhengying , Wu Xiuling , Liu Jinghong , Wang Yu , Zhang Yue , Wu Yan , Kang Yingxue , Zhang Ronghai , Li Jin , Liu Delong 
Published: November 2023

Background: Adenoidal hypertrophy (AH) is one of the most common causes of upper airway obstruction in children. Drug and surgical treatment are the typical treatment of AH. The study on the inflammatory mechanism of AH in children provides a new idea for preoperative intervention and non-surgical treatment with anti-inflammatory drugs such as montelukast sodium (a cysteine leukotriene receptor antagonist). The aim of this study is to evaluate the effect of montelukast sodium on adenoidal lymphoid tissue pathology in children with AH under light microscope.Objective: To study whether there is any change in pathology of the adenoidal lymphoid tissue under the light microscope compared with the control group in children with moderate to severe simple AH treated with montelukast sodium for 1 month before operation.Materials and methods: Twenty patients (8 males, 12 females, 3–8 years old) with moderate to severe AH who were prepared for surgical treatment were selected. All the patients were examined by Nasopharyngeal CT and hemocyte analysis before operation. 20 subjects were randomly divided into two groups: One group was given montelukast chewable tablets 5 mg/d, qn, for 4 weeks; The control group was given placebo 5 mg/d, qn, for 4 weeks. After 4 weeks, the adenoids were removed and examined histopathology.Results: Compared with the control group, the number of lymphocytes in the blood cell analysis of the study group was significantly reduced, with a statistically significant difference (p &lt; 0.05). And the number of germinal centers in adenoid tissue of the study group was relatively reduced, no small cyst was found in the epithelium, and the degree of inflammatory cell infiltration was reduced, with a statistically significant difference (p &lt; 0.05).Conclusion: Montelukast can reduce the number of reactive cells, the number of lymphocytes in blood cells and blood vessels in adenoid lymphoid tissue, which can provide a new idea for preoperative intervention and non-surgical treatment of adenoid hypertrophy in children. However, this is only a pilot study and a longer treatment period is needed to assess the long-term effects of montelukast sodium on adenoid lymphoid tissue.Clinical Trial Registration: www.Chictr.org.cn, identifier ChiCTR2300075040.
Pharmacological and mechanical properties of isolated pig coronary veins
Authors: Wang Bowen , Qin Zhi , Li Mei , Arner Anders , Steen Stig 
Published: November 2023

Recent successful cardiac transplantation from pig to non-human primates and the first pig-to-human transplantation has put the focus on the properties of the pig heart. In contrast to the coronary arteries, the coronary veins are less well characterized and the aim was to examine the mechanical and pharmacological properties of coronary veins in comparison to the arteries. Vessel segments from the left anterior descending coronary artery (LAD) and the concomitant vein were isolated from pig hearts in cardioplegia and examined in vitro. The wall thickness, active tension and active stress at optimal circumference were lower in coronary veins, reflecting the lower intravascular pressure in vivo. Reverse transcription polymerase chain reaction (RT-PCR) analysis of myosin isoforms showed that the vein could be characterized as having a slower smooth muscle phenotype compared to the artery. Both vessel types contracted in response to the thromboxane agonist U46619 with EC50 values of about 20 nM. The artery contracted in response to acetylcholine. Precontracted arteries relaxed in noradrenaline and substance P. In contrast, the veins relaxed in acetylcholine, contracted in noradrenaline and were unresponsive to substance P. In conclusion, these results demonstrate significant differences between the coronary artery and vein in the smooth muscle properties and in the responses to sympathetic and parasympathetic stimuli.
Reference values for body composition and physical fitness of young Brazilian elite soccer players
Authors: Portella Daniel Leite , Vidal-Espinoza Ruben , Sulla-Torres Jose , Castelli Correia de Campos Luis Felipe , Gomez-Campos Rossana , Cossio-Bolaños Marco 
Published: November 2023

Objective: a) to verify whether body composition and physical fitness should be analyzed by chronological age and/or maturity stage in young Brazilian soccer players and b) to propose reference values for the evaluation of body composition and physical fitness by maturity stage in elite soccer players.Methods: A descriptive-correlational study was carried out in 206 young Brazilian soccer players (11–16 years old). The sample selection was non-probabilistic by convenience. Weight and standing height were evaluated. Body composition (BC) was assessed by dual X-ray absorptiometry (DXA). BC indicators [(percent fat (%F), Fat-free mass (FFM), Fat mass (FM) and Bone mass (BM)] were extracted. For physical fitness (PF), we applied the Flexibility (cm) sit and reach tests, explosive strength tests [Counter Movement Jump CMJ (cm) and horizontal jump HJ (cm)], speed [Speed 10, 20, 30 and 40 m (seconds)] and Yo-Yo endurance level I test. Percentiles were constructed for BC and PF using the LMS method [L (Lambda; skewness), M (Mu; median) and S (Sigma; coefficient of variation)].Results: The explanatory power between chronological age (CA) with BC was: FM (R2 = 0.03%), FFM (R2 = 0.66%) and BM (R2 = 0.62%), while between maturity status (MS) with BC were: FM (R2 = 0.04%), FFM (R2 = 0.71%) and BM (R2 = 0.66). The explanatory power between the CA with the physical fitness tests ranged from: (R2 = 0.22–0.62%). While between MS with physical fitness the values ranged from: (R2 = 0.23–0.64%). Percentiles per MS (P3, P5, P10, P15, P25, P50, P75, P85, P90, P95 and P97) were proposed for both BC and PF.Conclusion: The results of the study have shown that the evaluation of BC and PF of young soccer players should be performed by controlling for MS rather than for CA. The inclusion of a non-invasive method to control MS by means of percentiles during puberty may contribute to the development of retention and exclusion of young soccer players, thus, they may have a better chance of achieving sporting success.
Trust in relationships: a preliminary investigation of the influence of parental divorce, breakup experiences, adult attachment style, and close relationship beliefs on dyadic trust
Authors: Yılmaz Ceren D. , Lajunen Timo , Sullman Mark J. M. 
Published: November 2023

IntroductionTrust is essential for establishing stable and fulfilling romantic relationships between partners. Development of trust, however, can be assumed to depend on many factors related to an individual's earlier experiences and relationship-related beliefs. This study aimed to investigate how adult attachment style (anxious, avoidant), experiences about parents' divorce and breakdown of one's own romantic relationship, and relationship beliefs are related to the level of dyadic trust in romantic relationships.MethodsThe present study included 131 Turkish undergraduate university students (55.7% women) from different faculties. The research instrument had questions about parents' and respondents' own relationship status, Dyadic Trust Scale (DTS), Experiences in Close Relationships Inventory-Revised (ECR-R), and Inventory of Close Relationship Beliefs (ICRB), in addition to background questions. The data were analyzed using descriptive statistics, t-tests, Pearson correlations, regression analyses and mediation analyses.ResultsRespondents whose parents had divorced or who had experienced a relationship breakdown had lower dyadic trust scores than those without these experiences. The trust scores correlated negatively with anxious and avoidant attachment styles and positively with relationship belief scales, although the correlations to “external factors” were not statistically significant. In regression analysis, anxious and avoidant attachment styles explained 42% and relationship beliefs 25% of the variance in trust. The only significant predictor among beliefs was “individuality.” Mediation analysis showed that the effects of anxious attachment style on trust were fully mediated by the relationship belief in “individuality.” The avoidant attachment style had a direct relationship to trust.DiscussionThe results show that anxious attachment style influences trust via relationship beliefs, while avoidant attachment style has a strong direct effect on trust as well as weaker effects via beliefs. The results are discussed in the context of Turkish culture and horizontal collectivism.
Loneliness Is Associated With Problematic Internet Use but Not With the Frequency of Substance Use: A Czech Cross-Sectional Study
Authors: Meckovsky Filip , Furstova Jana , Kosarkova Alice , Meier Zdenek , Tavel Peter , Malinakova Klara 
Published: November 2023

Objectives: This study aimed to assess the associations between loneliness and the frequency of substance use and problematic Internet use (PIU) in different age groups.Methods: Data were collected in April 2021 from a sample of 1,293 participants with main characteristics close to a nationally representative sample (mean age = 50.1 ± 15.4 years; 56% male). We measured loneliness with the Three-Item Loneliness Scale (TILS), PIU with the General Problematic Internet Use Scale-2 (GPIUS-2) and the frequency of drugs, alcohol, smoking or caffeine consumption. Spearman’s correlation, the t-test, and one-way and multivariate linear regression models were used to analyze the data.Results: In our study, 43.8% of respondents reported moderate to severe levels of loneliness. Loneliness was associated with the severity of PIU [F (3, 1,277) = 15.25, p &lt; 0.001], with higher loneliness corresponding to higher PIU. No significant relationship was found between loneliness and drugs, alcohol, smoking or caffeine consumption.Conclusion: Regardless of age, loneliness is associated with PIU but not with the frequency of substance use. Professional help for lonely people should focus on problematic Internet use in all age groups.
La Mission de Formation de Formateurs du C.R.E.D.I.F.
Authors: Poilroux Jean 
Published: November 2023

Le C.R.E.D.I.F. :
Le Centre de Recherche et d’Etude pour la Diffusion du Français a son origine dans la création en 1951 à l’Ecole Normale Supérieure de Saint-Cloud d’un Centre d’Etude, dirigé par le professeur Georges Gougenheim, dont les travaux ont abouti en 1954 à l’établissement d’un vocabulaire et d’une grammaire de base, le «Français Fondamental ».
Il constitue actuellement l’un des trois centres pédagogiques de l’E.N.S. de Saint-Cloud, établissement public à caractère scientifique et culturel dont le ministère de tutelle est le secrétariat d’Etat aux Universités. Il groupe environ 60 personnes (chargés de recherche, enseignants, personnels administratifs).
Après ses premiers travaux d’enquête et la publication du Français Fondamental (1er et 2e degrés), auxquels se sont greffés divers vocabulaires spécialisés, le C.R.E.D.I.F. s’est orienté vers la recherche et l’élaboration d’instruments pédagogiques, ainsi que la formation des enseignants, dans le domaine du français enseigné comme langue étrangère.
Les activités du Centre (recherche, production, formation), qui se sont longtemps attachées essentiellement à l’enseignement du français langue étrangère, se distribuent actuellement selon les cinq grandes orientations suivantes :
— l’apprentissage du français langue étrangère, à l’étranger ;
— la scolarisation des enfants de travailleurs migrants en France ;
— la formation des travailleurs migrants en France ;
— la formation continue : en particulier les besoins langagiers des adultes francophones en milieu professionnel en France ;
— l’enseignement du français dit «français fonctionnel » à l’étranger (notamment le français scientifique et technique).
Analisa Pengaruh Limbah Fly Ash Dan Battom Ash (Faba) Sebagai Pengganti Semen Dan Agregat Halus Terhadap Kuat Tekan Beton
Authors: Ulva  
Published: November 2023

Keterbatasan sumber daya alam untuk persediaan material penyusun beton karena bersumber dari alam yang tidak dapat di perbaharui. Maka perlunya inovasi dan pemanfaatan limbah untuk keperluan konstruksi. Pada limbah Fly Ash dan Battom Ash (FABA) ada beberapa unsur penyusun dan bentuk gradasi yang sama dengan semen dan agregat halus. Maka tujuan dari penelitian ini yaitu untuk menyelidiki penggunaannya untuk bahan pengganti semen dan dan agregat halus. Pada persentase 0%, 15%, 20% dan 25% dengan hasil nilai kuat tekan pada perawatan 28 hari mencapai 28,4 Mpa, 26,7 Mpa, 25,4 Mpa, dan 23,9 Mpa. Kuat tekan optimum terletak pada persentase 15% yaitu 26,7 MPa maka termasuk pada klasifikasi beton mutu sedang. Disimpulkan bahwa formula campuran beton eksperimental tersebut dapat digunakan di beberapa struktur yang dapat menggunakan mutu beton sedang. Diantaranya yaitu beton bertulang seperti plat lantai jembatan, gelagar beton bertulang, diafragma, beton pracetak, gorong- gorong beton bertulang, bangunan bawah jembatan
Contar los feminicidios en México: justicia, periodismo y archivos feministas en el siglo veintiuno
Authors: Osorio Ever E. 
Published: November 2023

En México, un país en guerra desde 2006 contra el ubicuo enemigo de “las drogas” mueren más de diez mujeres al día víctimas del feminicidio. ¿Cómo se expresa el dolor en este necroscenario? ¿Cómo se vive y se narra el duelo? ¿cómo se da cuenta de esas pérdidas que sabemos tenemos, pero que no logramos dimensionar como sociedad? Intentaré responder esta pregunta desde la escritura de las mujeres en México. A través del análisis de los libros El Invencible Verano de Liliana de Cristina Rivera Garza y La fosa del agua: desapariciones y feminicidios en el Rio de los Remedios, de Lydiette Carrión exploraré los archivos que se han construido para crear las memorias de las víctimas, que se convierten en formaciones discursivas que cuentan la experiencia de las violencias feminicidas y de estado. La memoria y la búsqueda de la verdad que plantean esta obras, son cruciales para la construcción de un discurso contracultural que responde a las necesidades afectivas de entender a estas violencias como un orden político que merma a todas las que vivimos en esta sociedad.