bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systemic allergic reaction to galcanezumab (emgality): a case report
Authors: Pico Michael J. , Badri Malaka , Michalak Nathan , Siefferman Jason W. 
Published: January 2024

Background A previously unreported systemic reaction to Galcanezumab (Emgality) is described. Galcanezumab is a humanized monoclonal antibody designed to bind to calcitonin gene-related peptide, a neuropeptide associated with neurogenic inflammation during migraine attacks. Although clinical trials showed that Galcanezumab had few adverse reactions (injection site related erythema, pruritus, and swelling), no systemic drug reactions have been noted. Case report A 50-year-old female with chronic migraine, mast cell disorder, Hashimoto’s disease, positive antinuclear antibody and positive anti-cyclic citrullinated peptide antibody not on immune modulators received the initial dose of galcanezumab 240 mg after failing multiple migraine treatments. The following day, she developed injection site reaction, malar erythema and flu-like symptoms. Symptoms progressed the second day after injection, and she developed swelling in her lips and throat. Intravenous steroid and antihistamines improved airway symptoms, and the remaining symptoms improved after a course of oral steroids. Conclusions Delayed system allergic reaction to Galcanezumab requiring emergency intervention may occur. A history of autoimmune disorder may be a predisposing factor.
The Anti-tumor Function of Shikonin by Targeting EGFR/AKT/mTOR Signaling in Human Osteosarcoma Cells
Authors: Zhu Rui-Dan , Zhang Jia-Jia , Yao Xiao-Bin , Wang Zhang-Jiao , Du Zhi-Ling , Xu Qing-Xia 
Published: January 2024

Background: Shikonin, a purified naphthoquinone separated from a Traditional Chinese medicinal herb Lithospermum erythrorhixon, which exhibits anticancer properties. Objectives: To clarify the molecular mechanisms of therapeutic effects of shikonin against osteosarcoma. Materials and Methods: Cell Counting Kit-8 (CCK-8) assay was employed to evaluate cell viability. Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) double-staining was conducted to evaluate the apoptotic ratio of the MG-63 cells. The effects of shikonin on the invasiveness of osteosarcoma cells were implemented by a transwell cell migration assay. In the meantime, a western blot assay was employed to detect alterations in the relevant mechanism proteins within osteosarcoma cells. Molecular docking analysis was conducted to anticipate the binding interaction between shikonin and EGFR/protein kinase B (AKT)/mTOR. Results: We observed that shikonin suppressed proliferation and induced apoptosis in the MG-63 cells in a dose-dependent manner. Pursuing these findings, the potential molecular mechanisms were detected. Shikonin intervention blocked epidermal growth factor receptor (EGFR) phosphorylation and decreased epidermal growth factor (EGF)-induced activation of downstream signaling molecules, such as AKT and mammalian target of rapamycin (mTOR) in the MG-63 cells. However, the additional recombinant human epidermal growth factor (rHuEGF) could stimulate the activation of EGFR/AKT/mTOR signaling and reverse cell apoptosis caused by shikonin. Molecular docking analysis showed that shikonin presented the highest bonding ability with EGFR, AKT, and mTOR. Conclusion: Our results show that shikonin inhibits human osteosarcoma development via inactivating EGFR/AKT/mTOR signaling. It demonstrates that shikonin may act as a potential therapeutic agent in osteosarcoma treatment.
Genetic Association of Lipids and Lipid-Lowering Drug Target Genes With Attention Deficit Hyperactivity Disorder
Authors: Guo Detong , Sheng Wenchao , Cai Yingzi , Shu Jianbo , Cai Chunquan 
Published: January 2024

Background: Lipid metabolism plays an essential role in nervous system development. Cholesterol deficiency leads to a variety of neurodevelopmental disorders, such as autism spectrum disorder and fragile X syndrome. There have been a lot of efforts to search for biological markers associated with and causal to ADHD, among which lipid is one possible etiological factor that is quite widely studied. We aimed to evaluate the causal relationship between lipids traits, lipid-lowering drugs, and attention deficit hyperactivity disorder (ADHD) outcomes using Mendelian randomization (MR) studies. Methods: We used summary data from genome-wide association studies to explore the causal relationships between circulating lipid-related traits and ADHD. Then, quantitative trait loci for the expression of lipid-lowering drug target genes and genetic variants associated with lipid traits were extracted. Summary-data-based MR and inverse-variance-weighted MR (IVW-MR) were used to investigate the correlation between the expression of these drug-target genes and ADHD. Results: After rigorous screening, 939 instrumental variables were finally included for univariable mendelian randomization analysis. However, there is no correlation between lipid profile and ADHD risk. Drug target analysis by IVW-MR method observed that APOB-mediated low-density lipoprotein cholesterol was associated with lower ADHD risk (odds ratio [ OR] = 0.90, 95% confidence interval [CI] [0.84, 0.97]; p = .007), whereas LPL-mediated triglycerides levels were associated with a higher risk of ADHD ( OR = 1.13, 95% CI [1.06, 1.21]; p < .001). Conclusion: Our results suggest that APOB gene and LPL gene may be candidate drug target genes for the treatment of ADHD.
Cannula associated deep vein thromboses in COVID-19 patients supported with VV ECMO
Authors: Niles Erin , Maldarelli Mary , Hamera Joseph , Lankford Allison , Galvagno Samuel M , Menne Ashley , Boswell Kimberly , Rector Raymond , Haase Daniel J , Tabatabai Ali , Powell Elizabeth K 
Published: January 2024

Background: VV ECMO is increasingly used as a rescue strategy for hypercarbic and hypoxic respiratory failure refractory to conventional management, and more than 14,000 patients with COVID-19 related respiratory failure have been supported with VV ECMO to date. One of the known complications of VV ECMO support is the development of cannula-associated deep vein thromboses (CaDVT). The purpose of this study was to identify the incidence of CaDVT in COVID-19 patients supported with VV ECMO as compared to non-COVID-19 patients. We hypothesized that due to the hypercoagulable state and longer duration of VV ECMO support required for patients with COVID-19, a higher incidence of CaDVT would be observed in these patients. Methods: This is a single center, retrospective observational study. About 291 non-trauma adult patients who were cannulated for VV ECMO and managed at our institution from January 1, 2014 to January 10, 2022 were included. The primary outcome was the presence of CaDVT 24 h after decannulation in COVID-19 versus non-COVID-19 patients. Our secondary outcome was continued presence of DVT on follow up imaging. CaDVT were defined as venous thrombi detected at prior cannulation sites. Results: Both groups had a high incidence of CaDVT. There was no significant difference in the incidence of CaDVT in COVID-19 patients compared to non-COVID-19 patients (95% vs 88%, p = 0.13). Patients with COVID-19 had an increased incidence of persistent CaDVT on repeat imaging (78% vs 56%, p = 0.03). Conclusion: Given the high number of post-decannulation CaDVT in both groups, routine screening should be a part of post ECMO care in both populations. Repeat venous duplex ultrasound should be performed to assess for the need for ongoing treatment given the high incidence of CaDVT that persisted on repeat duplex scans.
Final analysis of 379 pregnancy outcomes after exposure to dimethyl fumarate in a prospective international registry
Authors: Hellwig Kerstin , Rog David , McGuigan Christopher , Houtchens Maria K , Bruen Denise R , Mokliatchouk Oksana , Branco Filipe , Levin Seth , Everage Nicholas , Lin Xiaochen 
Published: January 2024

Background: Dimethyl fumarate (DMF) has a favorable benefit–risk profile treating people with multiple sclerosis and should be used in pregnant women only if the potential benefits outweigh potential risks to the fetus. Objective: Assess pregnancy outcomes in a completed international registry (TecGistry) of women with MS exposed to DMF. Methods: TecGistry included pregnant women with MS exposed to DMF, with data collected at enrollment, 6–7 months gestation, 4 weeks after estimated due date, and at postpartum weeks 4, 12, and 52. Outcomes included live births, gestational size, pregnancy loss, ectopic/molar pregnancies, birth defects, and infant/maternal death. Results: Of 397 enrolled, median (range) age was 32 years (19–43). Median (range) gestational week at enrollment was 10 (0–39) and at first DMF exposure was 1 (0–13). Median (range) duration of gestational DMF exposure was 5 weeks (0–40). Fifteen (3.8%) spontaneous abortions occurred. Of 360 (89.1%) live births, 323 were full term and 37 were premature. One neonatal death and no maternal deaths occurred. Adjudicator-confirmed EUROCAT birth defects were found in 2.2%. Conclusion: DMF exposure during pregnancy did not adversely affect pregnancy outcomes; birth defects, preterm birth, and spontaneous abortion were in line with rates from the general population.
Monitoring walking asymmetries and endpoint control in persons living with chronic stroke: Implications for remote diagnosis and telerehabilitation
Authors: Song Jiafeng , Hardin Elizabeth C 
Published: January 2024

Objective The objective of this study was to assess the feasibility of monitoring and diagnosing compromised walking motion in the frontal plane, particularly in persons living with the chronic effects of stroke (PwCS). The study aimed to determine whether active control of walking in the frontal plane could be monitored and provide diagnostic insights into compensations made by PwCS during community living. Methods The study recruited PwCS with noticeable walking asymmetries and employed a monitoring method to assess frontal plane motion. Monitoring was conducted both within a single assessment and between assessments. The study aimed to uncover baseline data and diagnostic information about active control in chronic stroke survivors. Data were collected using sensors during 6 minutes of walking and compared between the paretic and non-paretic legs. Results The study demonstrated the feasibility of monitoring frontal plane motion and diagnosing disturbed endpoint control ( p < 0.0125) in chronic stroke survivors when comparing the paretic leg to the non-paretic leg. A greater variability was observed in the paretic leg ( p < 0.0125), and sensors were able to diagnose a stronger coupling of the body with its endpoint on the paretic side ( p < 0.0125). Similar results were obtained when monitoring was conducted over a six-minute walking period, and no significant diagnostic differences were found between the two monitoring assessments. Monitoring did not reveal performance fatigue or debilitation over time. Conclusions This study's findings indicate that monitoring frontal plane motion is a feasible approach for diagnosing compromised walking motion. The results suggest that individuals with walking asymmetries, exhibit differences in endpoint control and variability between their paretic and non-paretic legs. These insights could contribute to more effective rehabilitation strategies and highlight the potential for monitoring compensations during various activities of daily living.
Development-induced Changes in the ‘Urban Landscape’ and ‘Social Space’: A Case Study of Metro Rail’s Impact on the Hyderabad City
Authors: Gopal Amajala Venu , Singh M. Romesh 
Published: January 2024

Scholars interested in urban space focus on topics such as place-making, place attachment, place identities and the politics of place. The expanded landscape of cities compels its inhabitants to constantly move into the city for livelihood and other activities. Consequently, urban anthropologists showed considerable interest in people’s mobility, infrastructure associated with mobility and mobility cultures in the city. However, in its dynamics, mobility alters the urban space, leading to the emergence of new frontiers for holistic urban phenomena. How urban space expansion causes mobility needs and the mobility solution, in turn, modifies urban space is the prime concern for the research. The present research focuses on the changes that occurred in the urban space with the onset of a single development programme introduced for people’s mobility needs. The study investigated ‘Metro’ commuters and non-commuters in Hyderabad City. Data for this urban ethnographic study of an Indian city were gathered using observation, semi-structured interviews and the case study approach. The study finds that Metro rail construction and operation for the mobility needs of the people have produced a new kind of techno-space on the roads of Hyderabad city. It also highlights how Metro pushed the ‘gentrification’ process to the forefront of urban social structural changes.
Kinetics of IL-6, C-reactive Protein and Fibrinogen Levels in COVID-19 Outpatients Who Evolved to Hypoxemia
Authors: Mendes-Filho Sérgio Paulo de Mello , de Souza Pinheiro Rebeca , Martins Fernanda Simão , Giroldi Paulo Jose , e Melo Raul Honorato , de Oliveira Edcleia Lopes , dos Santos Anibal Borin , Medeiros Dayse Cristina Oliveira , Lopes Jéssica Amaral , Chaves Yury Oliveira , Zuliani Juliana Pavan , Nogueira Paulo Afonso 
Published: January 2024

Introduction: Despite the efficacy of the COVID-19, the search for improvements in the management of severe/critical cases continues to be important. The aim is to demonstrate the kinetics of 4 serological markers in patients with COVID-19 who evolved in hypoxemia. Methods: From June to December 2020, the Health Secretariat of Rondônia State, Brazil, established a home medical care service team (HMCS) that provided clinical follow-up for health professionals and military personnel with COVID-19. The clinical and laboratory monitoring was individualized at home by a nursing and medical team. In addition to laboratory parameters, C-reactive protein (CRP), interleukin-6 (IL-6), fibrinogen, and D-dimer levels were periodically taken to monitor the evolution of treatment. Results: Of 218 patients telemonitored, 48 patients needed special care by the HMCS team due to shortness of breath. Chest tomography showed multiple ground-glass shadows and lung parenchymal condensations that was compatible with secondary bacterial infection associated with leukocytosis, for which antibiotics were prescribed. The symptoms were accompanied by increases of CRP and IL-6 levels followed by fibrinogen after a few days, for which an anticoagulant therapy was included. Thirty-three patients evolved to improvements in clinical signs and laboratory results. Between the sixth and eighth day of illness, 15 patients presented signs of hypoxemia with low O2 saturation accompanied with an increase in the respiratory rate, with some of them requiring oxygen therapy. As they did not present signs of clinical severity, but their laboratory markers showed an abrupt IL-6 peak that was higher than the increase in CRP and a new alteration in fibrinogen levels, they received a supplemental dose of anticoagulant and a high dose of corticosteroids, which resulted in clinical improvement. Conclusion: Our study demonstrates that monitoring of IL-6 and CRP may identify precocious hypoxemia in COVID-19 patients and prevented the progressive deterioration of the lung injury.
The potential role of ocular and otolaryngological mucus proteins in myalgic encephalomyelitis/chronic fatigue syndrome
Authors: Huitsing Kaylin , Tritsch Tara , Arias Francisco Javier Carrera , Collado Fanny , Aenlle Kristina K. , Nathason Lubov , Fletcher Mary Ann , Klimas Nancy G. , Craddock Travis J. A. 
Published: January 2024

AbstractMyalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating illness associated with a constellation of other symptoms. While the most common symptom is unrelenting fatigue, many individuals also report suffering from rhinitis, dry eyes and a sore throat. Mucin proteins are responsible for contributing to the formation of mucosal membranes throughout the body. These mucosal pathways contribute to the body’s defense mechanisms involving pathogenic onset. When compromised by pathogens the epithelium releases numerous cytokines and enters a prolonged state of inflammation to eradicate any particular infection. Based on genetic analysis, and computational theory and modeling we hypothesize that mucin protein dysfunction may contribute to ME/CFS symptoms due to the inability to form adequate mucosal layers throughout the body, especially in the ocular and otolaryngological pathways leading to low grade chronic inflammation and the exacerbation of symptoms.
A Dual-Task Learning Approach for Bearing Anomaly Detection and State Evaluation of Safe Region
Authors: Yin Yuhua , Liu Zhiliang , Guo Bin , Zuo Mingjian 
Published: January 2024

AbstractPredictive maintenance has emerged as an effective tool for curbing maintenance costs, yet prevailing research predominantly concentrates on the abnormal phases. Within the ostensibly stable healthy phase, the reliance on anomaly detection to preempt equipment malfunctions faces the challenge of sudden anomaly discernment. To address this challenge, this paper proposes a dual-task learning approach for bearing anomaly detection and state evaluation of safe regions. The proposed method transforms the execution of the two tasks into an optimization issue of the hypersphere center. By leveraging the monotonicity and distinguishability pertinent to the tasks as the foundation for optimization, it reconstructs the SVDD model to ensure equilibrium in the model's performance across the two tasks. Subsequent experiments verify the proposed method's effectiveness, which is interpreted from the perspectives of parameter adjustment and enveloping trade-offs. In the meantime, experimental results also show two deficiencies in anomaly detection accuracy and state evaluation metrics. Their theoretical analysis inspires us to focus on feature extraction and data collection to achieve improvements. The proposed method lays the foundation for realizing predictive maintenance in a healthy stage by improving condition awareness in safe regions.
Modeling of radial growth curves and radial variation of basic density in Chamaecyparis obtusa planted in two progeny test sites
Authors: Takahashi Yusuke , Ishiguri Futoshi , Matsushita Michinari , Nezu Ikumi , Ohshima Jyunichi , Yokota Shinso , Tamura Akira , Tsubomura Miyoko , Takahashi Makoto 
Published: January 2024

AbstractThe objectives of the present study are to clarify the effect of macro- and micro-environment on the radial growth patterns and radial variation patterns of basic density in hinoki cypress (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.). We evaluated the radial variation patterns of cumulative annual ring width (as radial growth pattern) and basic density by modeling methods using hinoki cypress 36 families planted at two progeny test sites. In addition, narrow-sense heritability and correlation between sites for annual ring width and basic density were investigated. As the results of modeling for radial growth patterns, radial growth patterns slightly differed between sites. In addition, the stem diameter reaching the plateau might be varied among blocks in a site. On the other hand, radial variation of basic density was affected by genetic factors rather than blocks in the site. However, the radial growth rate may somewhat affect the radial variation of basic density. The heritability and correlation coefficients between sites in basic density were higher than those of annual ring width. Therefore, although radial growth in hinoki cypress varies by the effects of micro- and macro-environmental factors and has some influence on the radial variation of basic density, basic density is more strongly affected by genetic factors than by these influences, allowing for effective improvement for wood density by tree breeding program.
The pyroptosis mediated biomarker pattern: an emerging diagnostic approach for Parkinson’s disease
Authors: Liang Junhan , Wan Zhirong , Qian Cheng , Rasheed Madiha , Cao Changling , Sun Jingyan , Wang Xuezhe , Chen Zixuan , Deng Yulin 
Published: January 2024

Abstract
Background
Parkinson’s disease (PD) affects 1% of people over 60, and long-term levodopa treatment can cause side effects. Early diagnosis is of great significance in slowing down the pathological process of PD. Multiple pieces of evidence showed that non-coding RNAs (ncRNAs) could participate in the progression of PD pathology. Pyroptosis is known to be regulated by ncRNAs as a key pathological feature of PD. Therefore, evaluating ncRNAs and pyroptosis-related proteins in serum could be worthy biomarkers for early diagnosis of PD.

Methods
NcRNAs and pyroptosis/inflammation mRNA levels were measured with reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Luciferase assays were performed to confirm GSDME as a target of miR-675-5p and HMGB1 as a target of miR-1247-5p. In the serum of healthy controls (n = 106) and PD patients (n = 104), RT-qPCR was utilized to assess miR-675-5p, miR-1247-5p, and two related ncRNAs (circSLC8A1and lncH19) levels. The enzyme-linked immunosorbent assay measured serum levels of pyroptosis-related proteins in controls (n = 54) and PD patients (n = 70).

Results
Our data demonstrated that miR-675-5p and miR-1247-5p significantly changed in PD neuron and animal models. Overexpressed miR-675-5p or downregulated miR-1247-5p could regulate pyroptosis and inflammation in PD neuron models. Using the random forest algorithm, we constructed a classifier based on PD neuron-pyroptosis pathology (four ncRNAs and six proteins) having better predictive power than single biomarkers (AUC = 92%). Additionally, we verified the performance of the classifier in early-stage PD patients (AUC ≥ 88%).

Conclusion
Serum pyroptosis-related ncRNAs and proteins could serve as reliable, inexpensive, and non-invasive diagnostic biomarkers for PD.

Limitations
All participants were from the same region. Additionally, longitudinal studies in the aged population are required to explore the practical application value of the classifier.