bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of a helmet type, home-use low-level light therapy device for chemotherapy-induced alopecia: study protocol for a randomized controlled trial
Authors: Wu Cong-Xian , Li Cheng-Hsin , Shiao Yi-Hsien , Cheng Huan-Yu , Wu Tsung-Han , Lee Chun-Hui , Chang Zi-Yu , Yeh Yuan-Chieh 
Published: December 2023

Abstract
Background
Alopecia is one of the most common adverse effects of chemotherapy. It reduces the patient’s self-esteem and quality of life and the effect of therapy. Scalp cooling is the only verified current method for prevention but success is not guaranteed, particularly after receiving anthracycline-based combinations.
Low-level light therapy has been clinically proven to inhibit the progress of androgenic alopecia. A previous study using human subjects shows limited benefits for low-level light therapy for patients who suffer chemotherapy-induced alopecia but an increase in the number of probes and the optimization of light sources may improve the efficacy. This study determines the efficacy of low-level light therapy for the prevention of chemotherapy-induced hair loss for patients with breast cancer using a randomized controlled trial.

Methods
One hundred six eligible breast cancer patients were randomly distributed into a low-level light therapy group and a control group, after receiving chemotherapy. Subjects in the low-level light therapy group received 12 courses of intervention within 4 weeks. Subjects in the control group received no intervention but were closely monitored.
The primary outcome is measured as the difference in the hair count in a target area between the baseline and at the end of week 4, as measured using a phototrichogram (Sentra scalp analyzer). The secondary outcomes include the change in hair count at the end of week 1, week 2, and week 3 and hair width at the end of week 1, week 2, week 3, and week 4, as measured using a phototrichogram, and the change in distress, the quality of life, and self-esteem due to chemotherapy-induced alopecia, at the end of week 4, as measured using a questionnaire.

Discussion
This study improves cancer patients’ quality of life and provides clinical evidence.

Trial registration
Registered at ClinicalTrials.gov—NCT05397457 on 1 June 2022.
Whole-brain modeling of the differential influences of amyloid-beta and tau in Alzheimer’s disease
Authors: Patow Gustavo , Stefanovski Leon , Ritter Petra , Deco Gustavo , Kobeleva Xenia ,   for the Alzheimer’s Disease Neuroimaging Initiative
Published: December 2023

Abstract
Background
Alzheimer’s disease is a neurodegenerative condition associated with the accumulation of two misfolded proteins, amyloid-beta (A$$\beta$$
β
) and tau. We study their effect on neuronal activity, with the aim of assessing their individual and combined impact.

Methods
We use a whole-brain dynamic model to find the optimal parameters that best describe the effects of A$$\beta$$
β
and tau on the excitation-inhibition balance of the local nodes.

Results
We found a clear dominance of A$$\beta$$
β
over tau in the early disease stages (MCI), while tau dominates over A$$\beta$$
β
in the latest stages (AD). We identify crucial roles for A$$\beta$$
β
and tau in complex neuronal dynamics and demonstrate the viability of using regional distributions to define models of large-scale brain function in AD.

Conclusions
Our study provides further insight into the dynamics and complex interplay between these two proteins, opening the path for further investigations on biomarkers and candidate therapeutic targets in-silico.
Neural cell engraftment therapy for sporadic Creutzfeldt-Jakob disease restores neuroelectrophysiological parameters in a cerebral organoid model
Authors: Williams Katie , Foliaki Simote T. , Race Brent , Smith Anna , Thomas Tina , Groveman Bradley R. , Haigh Cathryn L. 
Published: December 2023

Abstract
Background
Sporadic Creutzfeldt-Jakob disease (sCJD), the most common human prion disease, is a fatal neurodegenerative disease with currently no treatment options. Stem cell therapy for neurodegenerative diseases is emerging as a possible treatment option. However, while there are a few clinical trials for other neurodegenerative disorders such as Parkinson’s disease, prion disease cell therapy research has so far been confined to animal models.

Methods
Here, we use a novel approach to study cell therapies in sCJD using a human cerebral organoid model. Cerebral organoids can be infected with sCJD prions allowing us to assess how neural precursor cell (NPC) therapy impacts the progression of sCJD. After 90 days of sCJD or mock infection, organoids were either seeded with NPCs or left unseeded and monitored for cellular composition changes, prion infection parameters and neuroelectrophysiological function at 180 days post-infection.

Results
Our results showed NPCs integrated into organoids leading to an increase in neuronal markers and changes in cell signaling irrespective of sCJD infection. Although a small, but significant, decrease in protease-resistant PrP deposition was observed in the CJD-infected organoids that received the NPCs, other disease-associated parameters showed minimal changes. However, the NPCs had a beneficial impact on organoid function following infection. sCJD infection caused reduction in neuronal spike rate and mean burst spike rate, indicative of reduced action potentials. NPC seeding restored these electrophysiological parameters to the uninfected control level.

Conclusions
Together with the previous animal studies, our results support that cell therapy may have some functional benefit for the treatment of human prion diseases.
Assessing resolvability, parsability, and consistency of RDF resources: a use case in rare diseases
Authors: Zhang Shuxin , Benis Nirupama , Cornet Ronald 
Published: December 2023

Abstract
Introduction
Healthcare data and the knowledge gleaned from it play a key role in improving the health of current and future patients. These knowledge sources are regularly represented as ‘linked’ resources based on the Resource Description Framework (RDF). Making resources ‘linkable’ to facilitate their interoperability is especially important in the rare-disease domain, where health resources are scattered and scarce. However, to benefit from using RDF, resources need to be of good quality. Based on existing metrics, we aim to assess the quality of RDF resources related to rare diseases and provide recommendations for their improvement.

Methods
Sixteen resources of relevance for the rare-disease domain were selected: two schemas, three metadatasets, and eleven ontologies. These resources were tested on six objective metrics regarding resolvability, parsability, and consistency. Any URI that failed the test based on any of the six metrics was recorded as an error. The error count and percentage of each tested resource were recorded. The assessment results were represented in RDF, using the Data Quality Vocabulary schema.

Results
For three out of the six metrics, the assessment revealed quality issues. Eleven resources have non-resolvable URIs with proportion to all URIs ranging from 0.1% (6/6,712) in the Anatomical Therapeutic Chemical Classification to 13.7% (17/124) in the WikiPathways Ontology; seven resources have undefined URIs; and two resources have incorrectly used properties of the ‘owl:ObjectProperty’ type. Individual errors were examined to generate suggestions for the development of high-quality RDF resources, including the tested resources.

Conclusion
We assessed the resolvability, parsability, and consistency of RDF resources in the rare-disease domain, and determined the extent of these types of errors that potentially affect interoperability. The qualitative investigation on these errors reveals how they can be avoided. All findings serve as valuable input for the development of a guideline for creating high-quality RDF resources, thereby enhancing the interoperability of biomedical resources.
The early fossil record of Caturoidea (Halecomorphi: Amiiformes): biogeographic implications
Authors: López-Arbarello Adriana , Concheyro Andrea , Palma Ricardo M. , Aguirre-Urreta Beatriz 
Published: December 2023

AbstractCaturoidea is a clade of Mesozoic predatory ray-finned fishes which lived mainly in the Jurassic. The clade has a few records in the earliest Cretaceous and only two in the Triassic. Among the latter, specimen MPCA 632 Caturus sp. doubtfully from continental Early Triassic of Argentina, i.e., outside Europe, was particularly problematic in the light of the known fossil record of the group, which suggested their origin in the Western Tethys. The micropaleontological and geochemical analysis of bulk-rock samples of MPCA 632 allowed us to correct the provenance of the specimen which corresponds to Tithonian marine outcrops of the Vaca Muerta Formation, Neuquén, Argentina. Specimen MPCA 632 is excluded from Caturus and reclassified as Caturoidea sp. MPCA 632 might be a specimen of Catutoichthys olsacheri, the only caturoid known from the Vaca Muerta Formation (Los Catutos Member), but the fossils are not comparable and, thus, this hypothesis needs further study. Additionally, the first-hand study of the type material of the only other alleged Triassic caturoid, Furo insignis, in the Norian of Seefeld, Austria, led to the exclusion of this taxon from the Caturoidea. Consequently, the clade Caturoidea is restricted to the Jurassic–Lowest Cretaceous. After a modest evolution during the Early Jurassic, the group had its initial radiation and westward dispersion across the Hispanic Corridor during the Middle Jurassic and reached its maximal diversity during the Kimmeridgian and Tithonian.
Prolonged vs intermittent intravenous infusion of β-lactam antibiotics for patients with sepsis: a systematic review of randomized clinical trials with meta-analysis and trial sequential analysis
Authors: Li Xiaoming , Long Yi , Wu Guixin , Li Rui , Zhou Mingming , He Aiting , Jiang Zhengying 
Published: December 2023

Abstract
Background
The prolonged β-lactam antibiotics infusion has been an attractive strategy in severe infections, because it provides a more stable free drug concentration and a longer duration of free drug concentration above the minimum inhibitory concentration (MIC). We conducted this systematic review of randomized clinical trials (RCTs) with meta-analysis and trial sequential analysis (TSA) to compare the effects of prolonged vs intermittent intravenous infusion of β-lactam antibiotics for patients with sepsis.

Methods
This study was prospectively registered on PROSPERO database (CRD42023447692). We searched EMBASE, PubMed, and Cochrane Library to identify eligible studies (up to July 6, 2023). Any study meeting the inclusion and exclusion criteria would be included. The primary outcome was all-cause mortality within 30 days. Two authors independently screened studies and extracted data. When the I2 values < 50%, we used fixed-effect mode. Otherwise, the random effects model was used. TSA was also performed to search for the possibility of false-positive (type I error) or false-negative (type II error) results.

Results
A total of 4355 studies were identified in our search, and nine studies with 1762 patients were finally included. The pooled results showed that, compared with intermittent intravenous infusion, prolonged intravenous infusion of beta-lactam antibiotics resulted in a significant reduction in all-cause mortality within 30 days in patients with sepsis (RR 0.82; 95%CI 0.70–0.96; P = 0.01; TSA-adjusted CI 0.62–1.07). However, the certainty of the evidence was rated as low, and the TSA results suggested that more studies were needed to further confirm our conclusion. In addition, it is associated with lower hospital mortality, ICU mortality, and higher clinical cure. No significant reduction in 90-day mortality or the emergence of resistance bacteria was detected between the two groups.

Conclusions
Prolonged intravenous infusion of beta-lactam antibiotics in patients with sepsis was associated with short-term survival benefits and higher clinical cure. However, the TSA results suggested that more studies are needed to reach a definitive conclusion. In terms of long-term survival benefits, we could not show an improvement.
Comprehensive analysis of immune cell landscapes revealed that immune cell ratio eosinophil/B.cell.memory is predictive of survival in sepsis
Authors: Wang Lei , Zhang Guoan , Sun Wenjie , Zhang Yan , Tian Yi , Yang Xiaohui , Liu Yingfu 
Published: December 2023

Abstract
Background
Immune dysregulation is a feature of sepsis. However, a comprehensive analysis of the immune landscapes in septic patients has not been conducted.

Objectives
This study aims to explore the abundance ratios of immune cells in sepsis and investigate their clinical value.

Methods
Sepsis transcriptome data sets were downloaded from the NCBI GEO database. The immunedeconv R package was employed to analyze the abundance of immune cells in sepsis patients and calculate the ratios of different immune cell types. Differential analysis of immune cell ratios was performed using the t test. The Spearman rank correlation coefficient was utilized to find the relationships between immune cell abundance and pathways. The prognostic significance of immune cell ratios for patient survival probability was assessed using the log-rank test. In addition, differential gene expression was performed using the limma package, and gene co-expression analysis was executed using the WGCNA package.

Results
We found significant changes in immune cell ratios between sepsis patients and healthy controls. Some of these ratios were associated with 28-day survival. Certain pathways showed significant correlations with immune cell ratios. Notably, six immune cell ratios demonstrated discriminative ability for patients with systemic inflammatory response syndrome (SIRS), bacterial sepsis, and viral sepsis, with an Area Under the Curve (AUC) larger than 0.84. Patients with a high eosinophil/B.cell.memory ratio exhibited poor survival outcomes. A total of 774 differential genes were identified in sepsis patients with a high eosinophil/B.cell.memory ratio compared to those with a low ratio. These genes were organized into seven co-expression modules associated with relevant pathways, including interferon signaling, T-cell receptor signaling, and specific granule pathways.

Conclusions
Immune cell ratios eosinophil/B.cell.memory and NK.cell.activated/NK.cell.resting in sepsis patients can be utilized for disease subtyping, prognosis, and diagnosis. The proposed cell ratios may have higher prognostic values than the neutrophil-to-lymphocyte ratio (NLR).
Nanotechnology in leukemia: diagnosis, efficient-targeted drug delivery, and clinical trials
Authors: Salama Maha M. , Aborehab Nora M. , El Mahdy Nihal M. , Zayed Ahmed , Ezzat Shahira M. 
Published: December 2023

AbstractLeukemia is a group of malignant disorders which affect the blood and blood-forming tissues in the bone marrow, lymphatic system, and spleen. Many types of leukemia exist; thus, their diagnosis and treatment are somewhat complicated. The use of conventional strategies for treatment such as chemotherapy and radiotherapy may develop many side effects and toxicity. Hence, modern research is concerned with the development of specific nano-formulations for targeted delivery of anti-leukemic drugs avoiding toxic effects on normal cells. Nanostructures can be applied not only in treatment but also in diagnosis. In this article, types of leukemia, its causes, diagnosis as well as conventional treatment of leukemia shall be reviewed. Then, the use of nanoparticles in diagnosis of leukemia and synthesis of nanocarriers for efficient delivery of anti-leukemia drugs being investigated in in vivo and clinical studies. Therefore, it may contribute to the discovery of novel and emerging nanoparticles for targeted treatment of leukemia with less side effects and toxicities.
Unveiling microbiome changes in Mediterranean octocorals during the 2022 marine heatwaves: quantifying key bacterial symbionts and potential pathogens
Authors: Prioux Camille , Tignat-Perrier Romie , Gervais Ophélie , Estaque Tristan , Schull Quentin , Reynaud Stéphanie , Béraud Eric , Mérigot Bastien , Beauvieux Anaïs , Marcus Maria-Isabelle , Richaume Justine , Bianchimani Olivier , Cheminée Adrien , Allemand Denis , Ferrier-Pagès Christine 
Published: December 2023

Abstract
Background
Climate change has accelerated the occurrence and severity of heatwaves in the Mediterranean Sea and poses a significant threat to the octocoral species that form the foundation of marine animal forests (MAFs). As coral health intricately relies on the symbiotic relationships established between corals and microbial communities, our goal was to gain a deeper understanding of the role of bacteria in the observed tissue loss of key octocoral species following the unprecedented heatwaves in 2022.

Results
Using amplicon sequencing and taxon-specific qPCR analyses, we unexpectedly found that the absolute abundance of the major bacterial symbionts, Spirochaetaceae (C. rubrum) and Endozoicomonas (P. clavata), remained, in most cases, unchanged between colonies with 0% and 90% tissue loss. These results suggest that the impairment of coral health was not due to the loss of the main bacterial symbionts. However, we observed a significant increase in the total abundance of bacterial opportunists, including putative pathogens such as Vibrio, which was not evident when only their relative abundance was considered. In addition, there was no clear relation between bacterial symbiont loss and the intensity of thermal stress, suggesting that factors other than temperature may have influenced the differential response of octocoral microbiomes at different sampling sites.

Conclusions
Our results indicate that tissue loss in octocorals is not directly caused by the decline of the main bacterial symbionts but by the proliferation of opportunistic and pathogenic bacteria. Our findings thus underscore the significance of considering both relative and absolute quantification approaches when evaluating the impact of stressors on coral microbiome as the relative quantification does not accurately depict the actual changes in the microbiome. Consequently, this research enhances our comprehension of the intricate interplay between host organisms, their microbiomes, and environmental stressors, while offering valuable insights into the ecological implications of heatwaves on marine animal forests.
Metabolic changes preceding bladder cancer occurrence among Korean men: a nested case-control study from the KCPS-II cohort
Authors: Han Youngmin , Kim Unchong , Jung Keum Ji , Lee Ji-Young , Lee Kwangbae , Shin Sang Yop , Kimm Heejin , Jee Sun Ha 
Published: December 2023

Abstract
Background
Bladder cancer (BLCA) research in Koreans is still lacking, especially in focusing on the prediction of BLCA. The current study aimed to discover metabolic signatures related to BLCA onset and confirm its potential as a biomarker.

Methods
We designed two nested case-control studies using Korean Cancer Prevention Study (KCPS)-II. Only males aged 35–69 were randomly selected and divided into two sets by recruitment organizations [set 1, BLCA (n = 35) vs. control (n = 35); set 2, BLCA (n = 31) vs. control (n = 31)]. Baseline serum samples were analyzed by non-targeted metabolomics profiling, and OPLS-DA and network analysis were performed. Calculated genetic risk score (GRS) for BLCA from all KCPS participants was utilized for interpreting metabolomics data.

Results
Critical metabolic signatures shown in the BLCA group were dysregulation of lysine metabolism and tryptophan-indole metabolism. Furthermore, the prediction model consisting of metabolites (lysine, tryptophan, indole, indoleacrylic acid, and indoleacetaldehyde) reflecting these metabolic signatures showed mighty BLCA predictive power (AUC: 0.959 [0.929–0.989]). The results of metabolic differences between GRS-high and GRS-low groups in BLCA indicated that the pathogenesis of BLCA is associated with a genetic predisposition. Besides, the predictive ability for BLCA on the model using GRS and five significant metabolites was powerful (AUC: 0.990 [0.980–1.000]).

Conclusion
Metabolic signatures shown in the present research may be closely associated with BLCA pathogenesis. Metabolites involved in these could be predictive biomarkers for BLCA. It could be utilized for early diagnosis, prognostic diagnosis, and therapeutic targets for BLCA.
The relationship between pregnancy and birth experience with maternal-fetal attachment and mother-child bonding: a descriptive-analytical study
Authors: Moniri Monireh , Rashidi Fatemeh , Mirghafourvand Mojgan , Rezaei Mansour , Ghanbari-Homaie Solmaz 
Published: December 2023

Abstract
Background
Pregnancy and childbirth experience can be important factors for a pleasant relationship between mother and baby. This study assessed the relationship between the pregnancy and birth experience with maternal-fetal attachment (MFA) and mother-child bonding.

Methods
A descriptive-analytical study was conducted among 228 pregnant women in Tabriz, Iran February 2022 to March 2023. Using cluster random sampling method, we included 228 women with gestational age 28–36 weeks and followed them up until six weeks postpartum. Data were collected in two stages using the following questionnaires: Pregnancy Experience Scale (hassles and uplifts), Maternal-Fetal Attachment Questionnaire (during the third trimester of pregnancy), Postpartum Bonding Questionnaire, and Childbirth Experience Questionnaire (six weeks postpartum). Data were analyzed using Pearson’s correlation test and general linear model.

Results
The mean score of MFA was significantly higher among women with feelings of being uplifted during pregnancy [β (95% CI) = 1.14 (0.87 to 1.41); p < 0.001]. However, there was no statistically significant relationship between pregnancy hassles and MFA and mother-child bonding (p > 0.05). Also, there was no statistically significant relationship between childbirth experience and mother-child bonding (p > 0.05).

Conclusion
According to the results of this study, pregnancy uplifts have a positive role in improving MFA. Therefore, it is recommended to plan interventions to make pregnancy period a pleasant experience for mothers.
Contextual numismatics: a post-processual approach illustrated by application to Roman coins
Authors: Krmnicek Stefan 
Published: December 2023

Abstract
In this paper, ‘contextual numismatics’ is presented as the most recent and innovative research direction in the field of numismatics. In addition, its further potential for research and the limit of gaining knowledge are outlined. A historical overview of the gradual development of an archaeological-oriented approach to numismatic material serves as an introduction to the discussion. This is followed by a presentation of the distinguishing features compared to other research methods to study Roman coin finds, as well as a discussion of possible paths and goals in the further development of this approach to study these culturally significant objects.