bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitric oxide delays the postharvest nutritional quality decline of “Golden Hook” beans
Authors: He Xuelian , Wang Lihong , Watkins Christopher B. , Bai Chunmei , Ma Lili , Guo Susu , Han Lichun , Wang Hongwei , Wang Qing , Zuo Jinhua , Zheng Yanyan 
Published: January 2024

Abstract“Golden Hook” beans turn purple to different degrees due to the different compositions and contents of flavonoids during storage. At the same time, quality losses due to softening or pathogen invasion, and the deteriorations of flavor associated with carbohydrate metabolism occur. To reveal the effect of nitric oxide (NO) generated from sodium nitroprusside (SNP) on the postharvest quality of “Golden Hook” beans, we used metabolome, transcriptome, and assay for transposase‐accessible chromatin with high‐throughput sequencing approaches to compare beans at the time of treatment with untreated and SNP‐treated beans stored at 8°C for 12 days. The results showed that NO induced the expression of CERK1, FLS2, JAZ, and MYC2, stimulated the expression of polyphenol oxidase, CAT, and l‐ascorbate oxidase, the accumulation of flavonoids, and the synthesis of lignin in the antioxidant system, improving the ability of beans to resist stress while maintaining the balance of reactive oxygen species. High expressions of F3′H, FLS, INV, SUS, BCAT, and the accumulation of 6‐C‐methylquercetin‐3‐O‐rutinoside were associated with the maintenance of the fresh purplish red appearance of the beans in the SNP‐12d group, without loss of flavor. SNP treatment downregulated the transcription factors blimp‐1, OBP1, COG1, and AT1G6957 associated with bean senescence. These results provide insight into the postharvest quality control of “Golden Hook” beans.
NF‐κB1 promotes miR‐27a‐5p upregulation in acrylamide‐induced toxicity in rats
Authors: Zhang Lujia , Yang Liuqing , Gao Jianwei , Dong Li , Luo Yinghua , Chen Fang 
Published: January 2024

AbstractAcrylamide (AA) is a neurotoxic and potentially carcinogenic contaminant, usually generated during food processing. MicroRNAs are a class of noncoding small RNAs, which serve essential pathogenic roles in linking exposure to toxic contaminants with their pathological outcomes. Our previous research identified miR‐27a‐5p as a key regulator in AA toxicity, as it induces mitochondria‐dependent apoptosis by targeting Btf3 in rats. However, the mechanisms by which miR‐27a‐5p is regulated to exert its toxic effects remain unclear. Here, the transcription factors that bind to the promoter of miR‐27a‐5p and their potential regulatory functions are investigated in cell lines and rats. The results showed that nuclear factor kappa‐B 1 (NF‐κB1) is a promising transcription factor from bioinformatic analysis. The luciferase reporter assay demonstrated that NF‐κB1 enhances the transcriptional activity of the miR‐27a‐5p promoter, whereas the cleavage under targets and tagmentation assay successfully verified the specific binding sites at −305/−298 bp. Furthermore, the inhibition of NF‐κB1 resulted in the suppression of miR‐27a‐5p expression and its associated target pathways, with additional validation provided by in vivo experimentation. Consequently, the upregulation of miR‐27a‐5p induced by AA and its subsequent intrinsic apoptosis can be attributed to the binding of NF‐κB1 to the miR‐27a‐5p promoter. This binding event may serve as the initial step in the molecular network regulated by miR‐27a‐5p that underlies AA toxicity.
Therapeutic potential of dietary nutrients and medicinal foods against metabolic disorders: Targeting Akkermansia muciniphila
Authors: Xu Wenyi , Zhang Shaozhuo , Yang Yanan , Zhan Jiaguo , Zang Chenchen , Yu Huifang , Wu Chongming 
Published: January 2024

AbstractAs one of the most attractive next‐generation probiotics, mucin‐degrading Akkermansia muciniphila has emerged as an essential and integral factor in maintaining human health and affecting pathological outcomes. Its abundance is inversely associated with various metabolic diseases (e.g., obesity and type 2 diabetes), cardiovascular diseases, and intestinal inflammation. Supplementing A. muciniphila to restore the gut microbiota ecosystem is a promising approach for treating metabolic disorders. However, the direct utilization of this probiotic is limited by technological and regulatory hurdles, such as the in vitro bulk culture of A. muciniphila and the need for expensive animal‐derived materials. Therefore, enrichment of A. muciniphila using nutraceutical supplements is a feasible strategy. Dietary supplements, especially medicinal herbs, offer a vast and valuable resource as potential prebiotics for promoting the growth of A. muciniphila in the gut, ensuring reliable safety and efficacy. In this study, we first systemically reviewed the dietary substances and medicinal foods known to promote A. muciniphila from over 100 literature sources, aiming to establish a candidate basis for future exploration of prebiotics targeting A. muciniphila. Furthermore, we summarized and discussed the major regulatory factors and mechanisms responsible for the beneficial effect of A. muciniphila on metabolic disorders, hoping to open up exciting directions for in‐depth research on the pharmacological mechanism of A. muciniphila and pave the way for its clinical therapeutics.
Holothuria leucospilota polysaccharides (HLP) ameliorate colitis rats via regulation of the metabolic profiling and TLR4/NLRP3 signaling pathways
Authors: Zhang Xin , Shi Qiuge , Hu Maojie , Zhu Kexue , Zhu Lulu , Cao Jun , Li Chuan 
Published: January 2024

AbstractRecently, the development of natural polysaccharides for ameliorating immunity and gut metabolism has attracted extensive attention. This study used Holothuria leucospilota polysaccharides (HLP) to explore the improvement mechanism in ulcerative colitis rats on perspectives of immunity and metabolism. The results showed that HLP increased goblet cells’ number and the content of tight junction proteins (zona occludens 1 and occludin) and improved intestinal barrier permeability. The levels of immune cytokines (IL‐4, IL‐6, IL‐10, IL‐18, TNF‐α, and IL‐1β) and the activity of oxidative stress‐related enzymes (superoxide dismutase, catalase, malondialdehyde, and glutathione peroxidase) were regulated. HLP regulated the related genes and proteins expression of immune cytokines, MAPK, and NLRP3 inflammasome. Furthermore, HLP treatment increased the concentration of short‐chain fatty acids (SCFAs) and regulated serum metabolic disorders by regulating amino acid metabolism, SCFA metabolism, and energy metabolism. These results provide a new perspective for developing HLP as a promising functional food for preventing and mitigating colitis.
How to enhance the acceptability of insects food—A review
Authors: Okaiyeto Samuel Ariyo , Yu Shi‐Han , Deng Li‐Zhen , Wang Qing‐Hui , Sutar Parag Prakash , Wang Haiou , Zhao Jin‐Hong , Mujumdar Arun S. , Ni Jia‐Bao , Lv Weiqiao , Xiao Hong‐Wei 
Published: January 2024

AbstractAbout 1 billion people worldwide suffer from hunger, so exploring new food sources is very tempting for achieving zero hunger in the world. Edible insects (EIs) may be one of the ways to solve human protein deficiency. Currently, more than 200 species of EIs are consumed by over 2.5 billion people, especially in tropical regions, as part of their regular diets. However, there is still a large rejection in various parts of the world. In this review, we systematically summarize the factors behind the rejection of EIs as well as ways to improve the acceptability of EIs as alternative protein, essential vitamins, and mineral sources. The main goal of this research is to spread the knowledge of the benefits of eating EIs, consumer perception of insects, and enhance its acceptability as an alternative food. Sensory attributes, health‐related concerns, and sustainability issues are identified as the key factors affecting consumer acceptability of EIs. Conventional processing methods, such as blanching, drying, roasting, and fermentation, have been used in treating EIs to improve the quality and safety of EIs. Nine strategies were proposed to enhance the acceptability of insects as food, such as promoting food safety, encouraging product development, addressing cultural norms, enhancing the culinary experience, collaborating with restaurants, and increasing public awareness through education. The information in this work will shed more light on the consumption of EIs and pave the way for more research in this area.
Detection of food contaminants: A review of established rapid analytical techniques and their applications and limitations
Authors: Ling Zhiming , Yang Luyu , Zhang Wanqing , Yao Ting , Xu Hengyi 
Published: January 2024

AbstractFood contaminants have consistently posed a serious threat to human health and have caused various food safety problems. The most common techniques currently used for the detection of food contaminants, include instrumental and immunoassay methods; however, these traditional methods have shown some limitations when applied to the analysis of food contaminants. Therefore, in recent years, more and more researchers have been devoted to the development of rapid, reliable, sensitive, and suitable detection techniques for the detection of food contaminants in food matrices. In this review, we first classify the analytical methods proposed in recent years into colorimetric methods, fluorescence methods, photoelectrochemical methods, electrochemical methods, nucleic acid amplification methods, and other methods, and illustrate their detection principles. In addition, the paper highlights the strategies and recent research advances for each method through typical application examples. Finally, the future perspectives of these analytical methods are presented. We hope that this review will provide researchers with valuable insights to guide the sensitive detection of food contaminants.
Common and distinct BOLD correlates of Simon and flanker conflicts which can(not) be reduced to time‐on‐task effects
Authors: Wojciechowski Jakub , Jurewicz Katarzyna , Dzianok Patrycja , Antonova Ingrida , Paluch Katarzyna , Wolak Tomasz , Kublik Ewa 
Published: January 2024

AbstractThe ability to identify and resolve conflicts between standard, well‐trained behaviors and behaviors required by the current context is an essential feature of cognitive control. To date, no consensus has been reached on the brain mechanisms involved in exerting such control: while some studies identified diverse patterns of activity across different conflicts, other studies reported common resources across conflict tasks or even across simple tasks devoid of the conflict component. The latter reports attributed the entire activity observed in the presence of conflict to longer time spent on the task (i.e., to the so‐called time‐on‐task effects). Here, we used an extended Multi‐Source Interference Task (MSIT) which combines Simon and flanker types of interference to determine shared and conflict‐specific mechanisms of conflict resolution in fMRI and their separability from the time‐on‐task effects. Large portions of the activity in the dorsal attention network and decreases of activity in the default mode network were shared across the tasks and scaled in parallel with increasing reaction times. Importantly, the activity in the sensory and sensorimotor cortices, as well as in the posterior medial frontal cortex (pMFC) – a key region implicated in conflict processing – could not be exhaustively explained by the time‐on‐task effects.
Effects of rotation and temperature‐dependent viscosity on thermal convection in Oldroydian fluid saturating porous media: A modified stability analysis
Authors: Dhiman Joginder Singh , Gupta Khushboo , Sharma Praveen Kumar 
Published: January 2024

AbstractThe onset of thermal convection in an incompressible Oldroydian fluid‐saturating porous media is examined to study the combined impact of uniform rotation and temperature‐dependent viscosity. A characteristic equation from the basic hydrodynamic equations governing the Brinkman–Oldroyd model is derived using linear stability theory and modified Boussinesq approximation. For various combinations of stress‐free and slip‐free boundaries, the expressions for the Darcy–Rayleigh numbers for both non‐oscillatory as well as oscillatory convection with linear and exponential temperature‐dependent viscosity are derived, using the “weighted residual method.” The effects of rotation, variable viscosity parameter, strain retardation and stress relaxation time parameters and other fluid parameters on non‐oscillatory and oscillatory convection are investigated numerically and the results are presented graphically. From the analysis, it is found that overstability is the preferred mode of onset of convection. The rotation, the coefficient of specific heat variations (due to temperature variation), and the strain retardation time have a stabilizing influence on the stability of the system, whereas the stress relaxation imparts a destabilizing effect. Additionally, it is noticed that the variable viscosity parameter and Brinkman‐Darcy number stabilize the system for each set of boundary conditions.
Immune checkpoint molecules B7‐1 and B7‐H1 as predictive markers of pre‐eclampsia: A case–control study in a Ghana
Authors: Akilla Martin Awe , Nchor Ignatius Abowini Awinibuno , Banyeh Moses , Amidu Nafiu 
Published: January 2024

AbstractBackground/AimImmune tolerance in the fetal–maternal junction is maintained by a balance in the Th1/Th2 system. Th1‐type immunity is associated with pro‐inflammatory cytokines and immune checkpoint molecules (ICMs) such as B7‐H1, while Th2‐type immunity is characterized by anti‐inflammatory cytokines and ICMs such as B7‐1. Any imbalance in the Th1/Th2 immune system may lead to adverse pregnancy outcomes such as pre‐eclampsia (PE). Hitherto, the potential of serum B7‐1 and B7‐H1 proteins as early markers of PE has not been explored in the Ghanaian population.Materials and MethodsThis was a case–control study from May 2020 to April 2022 at the War Memorial and the Upper East Regional Hospitals. The study involved 291 women, including 180 (61.9%) with normotensive pregnancy and 111 (38.1%) with PE. Venous blood samples were collected and assayed for blood cell count, serum interleukins (ILs)‐4, ‐6, ‐12, ‐18, and TNF‐α as well as serum B7‐1 and B7‐H1 proteins.ResultsThe monocyte count (p = .007), the serum levels of IL‐18 (p = .035), TNF‐α (p = .001), and B7‐H1 (p = .006) were significantly higher in PE than in normotensive pregnancy. In addition, the monocyte count (p = .002), the serum levels of IL‐12 (p = .029), TNF‐α (p = .016), and B7‐1 (p = .009) levels were significantly higher in the third trimester than the second trimester PE. In predicting PE, the area under the curve of cytokines and ICMs ranged from 0.51 for IL‐6 to 0.62 for TNF‐α.ConclusionPE may be characterized by a dominant Th1‐type immunity with higher levels of pro‐inflammatory cytokines and B7‐H1 proteins, but these variables may not be suitable for predicting PE.
Sufficiently activated mature natural killer cells derived from peripheral blood mononuclear cells substantially enhance antitumor activity
Authors: Liu Chuanling , Li Yingying , Li Yanrong , Hu Meng , Wang Haiyan , Lu Shasha , Li Zhao , Dilimulati Dilinuer , Jiao Shunchang , Lu Shelian , Zhao Weihong 
Published: January 2024

AbstractBackgroundPeripheral blood‐derived natural killer (NK) cells spontaneously lyse tumor cells without prior sensitization. However, NK cells in peripheral blood (PBNK cells) are in a resting state and exhibit inhibitory phenotypes and impaired cytotoxicity. Thus, strengthening the cytotoxic effector function of PBNK cells and improving NK cell expansion in vitro for a convenient allogeneic therapy are essential.Materials and MethodsPure cytokine activation and expansion of NK cells (super NK [SNK]) from peripheral blood mononuclear cells were studied. Markers of activated and inhibited NK cells and cytokine secretion by NK cells were examined using flow cytometry. NK cell antitumor activity in vitro was assessed using lactate dehydrogenase (LDH) cytotoxicity assay and an Incucyte real‐time imaging system. Additionally, the function of SNK cells against ascites caused by ovarian cancer in NOD‐Prkdc(em26Cd52)il2rg(em26Cd22)/Nju (NCG) mice was determined. In a further investigation of the differences between PBNK and SNK, the mRNA of both cells was sequenced and analyzed.ResultsHuman peripheral blood mononuclear cells showed selective NK cell expansion upon cytokine activation and culture. Both SNK and PBNK cells expressed activation markers, but at different levels, and SNK cells secreted more cytokines related to cytotoxicity than PBNK cells did. Accordingly, SNK cells exhibited strong antitumor activity ex vivo and improved NCG mice survival after intraperitoneal ovarian cancer transplantation. Mechanistically, SNK cells expressed more genes associated with nucleotide metabolism, fatty acid, and ATP metabolism than PBNK cells.ConclusionSNK cells derived from peripheral blood mononuclear cells have sufficiently activated mature characteristics and high antitumor activity, rendering them a highly promising and essential therapeutic approach for cancer treatment.
A community based seroprevalence of SARS‐CoV‐2 antibodies in Somali Region, Eastern Ethiopia
Authors: Yared Solomon , Abera Tsegalem , Ali Seid Mohammed , Muhummed Abdifatah Muktar , Ibrahim Mohammed , Hussen Abdulahi , Hattendorf Jan , Zinsstag Jakob , Tschopp Rea 
Published: January 2024

AbstractBackgroundCoronavirus disease 19 (COVID‐19) is life‐threatening infectious disease caused by SARS‐CoV‐2 virus that caused a global pandemic. SARS‐CoV‐2 has been widely transmitted throughout Ethiopia, with over 501,060 cases confirmed and 7574 deaths until November 2023. This study assessed for the first time the seroprevalence SARS‐CoV‐2 in the general population of the Somali Region during the COVID‐19 pandemic.MethodsA cross‐sectional study design was conducted from May to June 2021 in 14 districts of Somali Region. Blood samples were collected in 820 participants in addition to administering a questionnaire that included sociodemographic characteristics and history of clinical symptoms of COVID‐19. Blood samples were tested for the presence or absence of anti‐SARS‐CoV‐2 using a commercial Enzyme‐Linked Immunosorbent Assay (ELISA) kit (Euroimmun).ResultsOverall, 477 (58.2%) were male and 343 (41.8%) were female. The majority of the participants (N = 581; 70.9%) were between 18 and 34 years old and not vaccinated against COVID‐19 (N = 793; 96.7%). The overall seroprevalence of SARS‐CoV‐2 antibodies was 41.7% (95% CI: 33.3%–47.6%). The highest prevalence was found in Goljano district (70%) and the lowest in Gunagado district (22.5%). Only age was found to be associated with COVID‐19 seropositivity.ConclusionPrevalence of SARS‐CoV‐2 antibodies was the highest ever reported in Ethiopia, indicating that a large proportion of the population had been infected 14 months after the start of the outbreak in the country. Such studies are important to swiftly reassess and improve specific COVID‐19 preventive and control measures to reduce transmissions within the community in a given setting.
Scientific approaches toward improving cervical cancer elimination strategies
Authors: Lehtinen Matti , Bruni Laia , Elfström Miriam , Gray Penelope , Logel Margaret , Mariz Filipe Colaço , Baussano Iacopo , Vänskä Simopekka , Franco Eduardo L. , Dillner Joakim 
Published: January 2024

AbstractAt the 2023 EUROGIN workshop scientific basis for strategies to accelerate the elimination of cervical cancer and its causative agent, human papillomavirus (HPV) were reviewed. Although some countries have reached key performance indicators toward elimination (>90% of girls HPV vaccinated and >70% of women HPV screened), most are yet to reach these targets, implying a need for improved strategies. Gender‐neutral vaccination, even with moderate vaccination coverage was highlighted as a strategy to achieve elimination more rapidly. It is more resilient against major disturbances in vaccination delivery, such as what happened during the coronavirus pandemic. Further, an analysis of ethical/legal issues indicated that female‐restricted vaccination is problematic. Extended catch‐up of vaccination with concomitant screening, and outreach to vulnerable groups were highlighted. Although birth cohorts with high coverage of HPV vaccination at school are protected against HPV, and HPVs have a very low reproductive rate in women above age 35, adult women below age 30 have inadequate direct protection. In addition to herd protection from gender‐neutral vaccination, this group can be protected by offering concomitant catch‐up HPV vaccination and HPV screening. Furthermore, hepatitis B vaccination experiences indicate that elimination cannot be achieved without prioritizing vulnerable/migrant populations. The long‐lasting durability of vaccination‐induced antibody responses suggests prolonged protection with HPV vaccines when adequately administrated. Finally, cost‐effectiveness modelling suggests that high‐coverage HPV vaccination in multiple population segments will be resource‐saving due to reduced need for screening. In summary, the workshop found that strategically optimal deployment of vaccination will accelerate elimination of HPV and cervical cancer.
A case of complete response to avelumab plus axitinib combination therapy for metastatic clear cell renal cell carcinoma in a kidney undergoing dialysis
Authors: Shiomi Ei , Goto Yuta , Hisano Mizuki , Ito Rento , Moriwaka Makoto , Ikarashi Daiki , Maekawa Shigekatsu , Kato Renpei , Kanehira Mitsugu , Ujiie Takashi , Obara Wataru 
Published: January 2024

IntroductionCombination therapies of immune checkpoint and tyrosine kinase inhibitors for end‐stage kidney disease and patients on hemodialysis need careful consideration as few case reports provide suitable management decisions.Case presentationA 70‐year‐old man who had undergone hemodialysis for 6 years due to nephrosclerosis. Avelumab plus axitinib combination therapy was performed for repeated lung metastasis, and a complete response was achieved without major side effects.ConclusionA complete response was achieved after Ave plus Axi combination therapy for clear cell renal cell carcinoma in a patient undergoing dialysis. This suggests that Ave plus Axi combination therapy may be safe and effective for dialysis patients.
Color analysis and tracking of the reduction of an indigo solution in real‐time using LabVIEW and machine vision
Authors: Chiang Hui‐Yu , Lin Cheng‐Huang 
Published: January 2024

AbstractA novel detection method, known as RGB‐tracking, was utilized to quantify the relative color changes in reactants/products during the dyeing of a solution of indigo as a model compound. The RGB‐tracking system consists of a digital camera, a light source, a data acquisition device, a LabVIEW program, and its built‐in function (Machine Vision). For the reduction of indigo, two types of reagents were employed: sodium dithionite (Na2S2O4) for the chemical redox process and yeast (Saccharomyces cerevisiae) for the biological fermentation process. As a result, in the case of chemical redox during the reduction of the indigo solution, the progress of the reaction can be described by a color change, that is, the curves of R‐ and G‐pixels, respectively. When the curve for the G‐pixels began to increase, this indicated that leuco‐indigo is being formed. A dramatic increase in the R‐curve indicates that the amount of leuco‐indigo has exceeded than that of indigo itself and that the reaction will gradually reach equilibrium. In contrast to this, additional complex behavior was observed in the case of biological fermentation. When the RGB‐tracking curves for the R‐ and G‐pixels reach their maximum values, this indicates that the concentration of leuco‐indigo was significantly higher than that of indigo itself. The flattening of the RGB‐tracking curves indicates that the reaction has reached equilibrium.