bluuebunny/crossref_metadata_2025_split huggingface dataset:
NUPR1 packaged in extracellular vesicles promotes murine triple-negative breast cancer in a type 1 interferon-independent manner
Authors: Ortiz Angelica , Stavrou Aikaterini , Liu Shan , Chen Danqi , Shen Steven S. , Jin Chunyuan 
Published: January 2024

Aim: This study aims to elucidate the involvement of triple-negative breast cancer (TNBC)-derived extracellular vesicles in metastasis. The loss of components in the type 1 interferon (IFN1) signaling pathway has been linked to the promotion of metastasis. However, IFN1 signaling induces immunological dormancy and promotes tumorigenesis. Our hypothesis was that TNBC cells release tumor-derived extracellular vesicles (TEVs) that promote metastasis in an IFN1-independent manner.
Methods: Two murine TNBC models and transgenic mice were used to examine the role of IFN1 in TNBC progression to metastasis. Reserpine was employed to determine the effect of TEV education on TNBC progression and overall survival. EVs from cancer cells treated with vehicle and reserpine and from the serum of tumor-bearing mice receiving reserpine were examined to determine changes in EV release and EV content.
Results: TNBC cells progress to metastasis in mice lacking the IFN1-induced gene cholesterol-25 hydroxylase (CH25H) or expressing the IFNAR1S526 knock-in that cannot be downregulated. Reserpine suppresses EV release from TNBC cells in vitro and in vivo . Western blot analysis demonstrated reserpine decreased NUPR1 protein levels in EVs. RNAseq analysis demonstrated that endothelial cells lacking CH25H treated with TEVs exhibited increased NUPR1 expression that was decreased by adding reserpine with the TEVs. NUPR1 overexpression upregulated genes that mediate TEV biogenesis and incorporation. Knockdown of NUPR1 with shRNA decreased the release of TEVs.
Conclusion: In conclusion, our study suggests that TNBC is driven by aberrant packaging of NUPR1 into TEVs which were transferred into recipient cells to activate pro-metastatic transcription driven by NUPR1.
Efeito de diferentes formas de aplicação de Trichoderma na produção de mudas de beterraba
Authors: Jantsch Felipe Talian , Silva Vanessa Neumann , Mello Emely de Souza , Prior Alisson Vedana 
Published: January 2024

A beterraba é uma hortaliça importante para alimentação, tendo uma boa aceitação devido ao seu sabor adocicado. A produção de mudas é uma etapa importante no cultivo de beterraba e estudar formas de se otimizar a eficiência de produção é importante, especialmente visando que a muda esteja apta a enfrentar as condições adversas encontradas no campo. Neste contexto, o objetivo desta pesquisa foi avaliar a influência das diferentes formas de aplicação de Trichoderma no processo de produção de mudas de beterraba. Foram testadas diferentes formas de aplicação de Trichoderma no processo de produção de mudas de beterraba. O experimento foi realizado na estufa agrícola da UFFS campus Chapecó. Os tratamentos utilizados foram: sem aplicação de Trichoderma harzianum (testemunha), aplicação de Trichoderma harzianum no tratamento de sementes, no substrato no momento da semeadura e aos 7 dias após a semeadura (DAS). Foram avaliados: emergência de plântulas, altura da parte aérea, número de folhas/muda, comprimento de raízes, massa seca da parte aérea e de raízes de mudas. Os resultados obtidos foram submetidos à análise de variância e comparação de médias (p<0,05). Assim, conclui-se que a aplicação de Trichoderma harzianum cepa ESALQ-1306 proporciona efeitos positivos na produção de mudas de beterraba, cultivar Chata do Egito, nas condições em que foi realizada essa pesquisa; de forma geral, a aplicação no substrato (T2 e T3) proporcionaram melhor desempenho de mudas, na maioria dos parâmetros avaliados.
Genetic Marker Detection of Natural-Focal Infectious Disease Pathogens in Samples of Ixodidae Ticks, Collected on the Territory of the Republic of Guinea
Authors: Naidenova E. V. , Zakharov K. S. , Kartashov M. Yu. , Agafonov D. A. , Senichkina A. M. , Katyshev A. D. , Diallo M. A. , Bah M. B. , Boumbaly S. , Kutyrev V. V. 
Published: January 2024

The circulation of a rather wide range of pathogens of natural-focal infectious diseases transmitted by ticks was detected in West Africa at different points of time: Borrelia, Rickettsia, Coxiella, Crimean-Congo hemorrhagic fever (CCHF), Bhanja, and bluetongue viruses, etc. Current epidemiological and epizootiological situation on natural-focal infectious diseases on the territory of the Republic of Guinea is not entirely clarified.The aim of this work was to identify genetic markers (RNA/DNA) of natural-focal infectious disease agents in samples of Ixodidae ticks collected in the Republic of Guinea, and to determine the spectrum of pathogens circulating in various landscape-geographical zones of the country.Materials and methods. To conduct research on the territory of all landscape-geographical zones of the Republic of Guinea, 4695 specimens of Ixodidae ticks of 11 species were collected. Taking into account the species appurtenance, gender, phase of development, as well as the site of collection, a panel of 1645 samples was compiled. Genetic markers of Crimean-Congo hemorrhagic fever and tick-borne encephalitis viruses, as well as Borrelia burgdorferi s.l., Ehrlichia chaffeensis, Ehrlichia muris, Anaplasma phagocytophilum, Coxiella burnetii, Rickettsia of the tick-borne spotted fever (TBSF) group, and Francisella tularensis were detected using polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR).Results and discussion. The following markers of natural-focal disease agents were found in the Ixodidae tick suspensions: DNA of Rickettsia of the TBSF group (25.6 % of all samples studied), DNA of C. burnetii (6.2 %), cDNA of B. burgdorferi s.l. (9.1 %), and RNA of the CCHF virus (2.5 %). The listed spectrum of pathogens has been registered in all landscape-geographical zones of Guinea. Genetic markers of tularemia, anaplasmosis, ehrlichiosis and tick-borne encephalitis pathogens have not been identified in this study. The results obtained made it possible to clarify the probable spectrum of tick-borne diseases in the territory of the Republic of Guinea, determined the need for further study of the circulation of natural-focal infectious disease agents in West Africa and conducting regular epizootiological monitoring.
Variability of <I>omp25</I> and <I>omp2a</I> Genes in Brucella Strains of Different Taxonomic Position
Authors: Osina N. A. , Boolgakova E. G. , Osin A. G. , Shvidenko I. G. , Shcherbakova S. A. 
Published: January 2024

The aim of the study was to identify structural differences in the Brucella omp25 and omp2a genes, which make it possible to determine their taxonomic position.Materials and methods. The objects of the study were the nucleotide sequences of the complete genomes of 48 Brucella strains presented in the GenBank NCBI database. To assess sequence homology, the BLAST algorithm and the MEGA 11 program were used.Results and discussion. In 13 species of Brucella and Brucella spp. with unknown species appurtenance, HindIII, EcoRV and EcoRI, AluI restriction profiles of genome regions have been reproduced in silico, including, omp25 and omp2a genes, flanked by primers 25A-25B and 2AB-2AA, respectively. In the omp25 gene, 11 non-synonymous and 24 synonymous mutations have been detected; two deletions: ∆103–108 bp – in В. nosferati, ∆562–597 bp – in В. Ovis; and ACT insertion after 585 bp – in В. vulpis. The variability of the omp2a gene in the studied Brucella strains was significantly higher. 138 SNPs have been identified, of which 60 lead to amino acid substitutions, 2 – form stop codons, and an additional deletion ∆424–561 bp – in В. abortus 1, 2, 4 biovars. Single mutations in the omp2a and omp25 genes had both group specificity – for several species, and unique – for a specific species or biovar of the pathogen. Allelic profiles of the omp25 and omp2a genes have greater resolution than their restriction profiles studied. The identified changes in the structure of the omp25 and omp2a genes correlate with the circulation of individual Brucella species and biovars in the organism of certain carriers. These genes show the presence of deletions, insertions and single polymorphic nucleotides specific to species, groups of species and, in some cases, biovars of the pathogen.
Assessment of Cholera Bacteriophages Mixture Safety on an Experimental Animal Model
Authors: Tyurina A. V. , Gaevskaya N. E. , Sinel’nik E. A. , Ivanova I. A. , Filippenko A. V. , Omel’chenko N. D. , Trufanova A. A. , Pogozhova M. P. , Anoprienko A. O. , Pasyukova N. I. 
Published: January 2024

The spread of Vibrio cholerae strains with multiple antibiotic resistance necessitates the search for alternatives to antibiotics. Cholera bacteriophages can become such means.The aim of the work was to study the safety of a mixture of cholera bacteriophages using an experimental animal model.Materials and methods. We used a composition based on cholera phages Rostov-M3, Rostov-13, FB1. The toxicity of the drug, cytotoxic and apoptogenic effects in experimental animals were assessed.Results and discussion. It has been shown that a single administration of the maximum defined dose of the mixture of phages does not cause negative effects on the body of laboratory animals. Comparison with the control group has not revealed statistically significant differences in the histological picture in the organs of the experimental animals after long-term administration of the drug. Also, after a single and repeated seven-day administration, these phages do not cause apoptosis and necrosis of immune competent cells in experimental animals. The results of the studies indicate the safety of the 3-component phage composition and can be used for the further development of new biological products based on cholera bacteriophages in the future.
Modern Diagnostic Technologies in the Study of the Field Material Collected from the Natural Plague Foci of the Kyrgyz Republic in 2023
Authors: Berdiev S. K. , Eroshenko G. A. , Balykova A. N. , Usenbaev N. T. , Kebekbaeva N. T. , Dzhaparova A. K. , Mukanmetesen uulu Zh. , Zhumashov D. , Razhapbaeva A. Sh. , Yuldasheva A. M. , Oglodin E. G. , Katyshev A. D. , Kuznetsov A. A. , Fadeeva A. V. , Kutyrev V. V. 
Published: January 2024

The aim of the work was to analyze the results of investigating the field material obtained from natural plague foci of the Kyrgyz Republic (KR) in 2023, using modern diagnostic technologies.Materials and methods. 1435 biological samples from the Tien Shan, Alai and Talas high-mountain foci of the Kyrgyz Republic were studied using conventional methods of laboratory diagnosis of plague: microbiological, immunological, biological; as well as modern molecular-genetic methods. Testing of the obtained samples for the presence of plague pathogen DNA was carried out using RT-PCR; and the presence of antibodies to the plague microbe was detected by enzyme-linked immunosorbent assay (ELISA). Molecular identification of Yersinia pestis strains according to their appurtenance to subspecies, biovars, and phylogenetic lineages was performed by RT-PCR using the method of identifying single-nucleotide substitutions based on the analysis of melting curves of products.Results and discussion. An approach to the molecular identification of Y. pestis strains from plague foci of the KR has been developed and validated through identifying singlenucleotide substitutions using the analysis of product melting curves (HMR-analysis) with a set of designed primers. It has been established that Y. pestis strains isolated in the Sarydzhaz autonomous focus of the Tien Shan high-mountain focus belong to the biovar antiqua of the main subspecies, phylogenetic branch 0.ANT5. The phylogenetic relation of Y. pestis strains isolated in 2023 was studied based on genome-wide SNP analysis. Areas of epizootic activity in Eastern Alai have been identified. The data obtained indicate the sustained activation of plague foci in the KR. Areas of the territory of the KR that are promising for paleogenomic research are also discussed.
Modern Features of Pathogenic Leptospira Isolation and Identification in Siberia and the Far East
Authors: Breneva N. V. , Kiseleva E. Yu. , Sharakshanov M. B. , Borisov S. A. , Budaeva S. E. , Balakhonov S. V. 
Published: January 2024

Recently, pathogenic Leptospira culture isolation is an extremely rare phenomenon in Russia.The aim of our work was to synthesize the lessons learned at the Irkutsk Anti-Plague Institute from Leptospira culture isolation and identification since 2011.Materials and methods. Material from eight individuals with suspected leptospirosis and from 942 small mammals (SM) was examined using PCR and microscopic agglutination test (MAT), from humans and 260 SM, applying bacteriological method. Bacteriological temperature test, MAT, PCR, MLST and MALDI-TOF mass spectrometry with the original Leptospira protein profiles base were used to identify cultures. Six complete genomes were generated at the Central Research Institute of Epidemiology of the Rospotrebnadzor.Results and discussion. Leptospira have not been isolated from humans against the background of taking antibiotics, despite the positive PCR and MAT results. Four cultures of Leptospira borgpetersenii of the Javanica serogroup and three L. kirschneri (Grippotyphosa) have been isolated from SM. The results of identification using MLST scheme No. 1 and MALDI-TOF MS are identical. MLST in silico has shown the uniformity of two Grippotyphosa serogroup strains from Primorie and Khabarovsk with a sequence-type (ST) profile 110:100:94. ST146 is determined in four Javanica serogroup strains according to scheme No. 1, and unique single nucleotide polymorphisms are detected according to schemes No. 2–3. Thus, in Siberia and the Far East, between 2012 and 2016, seven pathogenic Leptospira cultures were isolated from carriers in natural foci; carrier infection rate being12.0–48.9 %. Javanica serogroup strains differ in the MLST profile characteristics and adapt to nutrient media for a longer time than Grippotyphosa serogroup strains.
Serological Monitoring of Pandemic Influenza Virus Markers in the Russian Federation in 2021–2023
Authors: Ilyicheva T. N. , Moiseeva A. A. , Ivanova K. I. , Azaev M. Sh. , Marchenko V. Yu. 
Published: January 2024

State Scientific Center of Virology and Biotechnology “Vector” has been monitoring highly pathogenic influenza since 2005.The aim of this work was to track the markers of highly pathogenic influenza in the blood sera of people who had a contact with infected and/or deceased birds, as well as of residents from regions where emergence of new variants of influenza A virus is most likely to occur.Materials and methods. Sera were studied using hemagglutination inhibition test (HI test). HI-positive sera were subjected to virus neutralization reaction.Results and discussion. In 2021, 2076 blood serum samples from 19 regions of Russia were collected. Only 7 samples demonstrated significant titers in HI test with A/H5N8 viruses. In 2022, 1620 blood serum samples from 23 regions were obtained; 25 of them were positive for influenza А/H5N8 and А/H5N1 viruses. In 2023 (January-August), 3335 serum samples from 31 regions of the Russian Federation were collected. 28 samples were positive for influenza А/H5N8 and А/H5N1 viruses. Furthermore, we monitored blood sera for low-pathogenic A/H9N2 virus. The number of positive samples in 2021 was lower than 1 % (13 out of 2076); in 2022, it reached 5 % (81 out of 1620); in 2023, the share was lower than 1 % (31 out of 3335). The data obtained suggest indirectly that currently there is no stable circulation of zoonotic influenza A/H5N8 and A/H5N1 viruses in Russia. Influenza viruses A/H9N2 have widely spread in many countries of the world and actively participate in evolution of highly pathogenic influenza A/H5Nx viruses. The Russian Federation demonstrates a gradual increase in the number of blood serum samples with antibodies to A/H9N2 virus.
Antibiotic Resistance Patterns in Shiga Toxin-Producing <I>Escherichia coli</I> (STEC), <I>Salmonella</I>, <I>Shigella</I> and <I>Staphylococcus aureus</I> Isolated at Two Communal Kitchens Located in Hanoi City, Vietnam
Authors: Le Thi Lan Anh , Ta Thi Loan , Dinh Thu Minh , Vu Thi Thuong , Pham Thi Ha Giang , Bui Thi Thanh Nga , Pham Viet Hung , Nguyen Ngoc Tan , Trieu Phi Long , Le Thi Van Anh , Hoang Dang Hieu 
Published: January 2024

The aim of our study was to investigate the antibiotic resistance profiles of Shiga toxin-producing Escherichia coli (STEC), Salmonella spp., Shigella spp., and Staphylococcus aureus strains.Materials and methods. 660 samples were collected at two communal kitchens in Hanoi, Vietnam between 2021 and 2022. They included foodstuffs, environmental (food processing tools) and biological ones (swabs from the hands of personnel). The VITEK® 2 Compact system in combination with DNA sequencing was used to identify bacterial species. The antibiotic susceptibility test (AST) was performed according to Kirby-Bauer Disk Diffusion Susceptibility protocol following Clinical & Laboratory Standards Institute (CLSI) method (M100-Ed32).Results and discussion. In total, 53 pathogenic bacterial strains have been detected, including 11 STEC, 24 Salmonella enterica, 9 Shigella sonnei, Shigella flexneri, and 8 S. aureus. AST of STEC has showed the highest resistance rates to tetracycline and chloramphenicol (90.9 %); trimethoprim+sulfamethoxazole (81.8 %); ampicillin, gentamycin and piperacillin (63.6 %). The STEC isolates were susceptible to carbapenem group. Among the Salmonella strains, 50 % demonstrated resistance to ampicillin, followed by tetracycline and piperacillin (45.8 %). Additionally, 25 % were resistant to ticarcillin+clavulanic acid, 20.8 % – to trimethoprim+sulfamethoxazole, and 16.7 % – to chloramphenicol. All Salmonella strains exhibited susceptibility to gentamicin, cefoxitin, imipenem, meropenem, and ceftazidime. AST of Shigella strains revealed the highest resistance rate for tetracycline (30 %), followed by cefazolin and ceftazidime (20 %). However, all Shigella strains were susceptible to cefoxitin, carbapenem groups, and chloramphenicol. Among the S. aureus strains, 50 % exhibited resistance to erythromycin, azithromycin, clindamycin, penicillin, telithromycin, and gentamicin, followed by ciprofloxacin, moxifloxacin, levofloxacin, and chloramphenicol (25 %). All S. aureus strains were still susceptible to trimethoprim+sulfamethoxazole, daptomycin, linezolid, doxycycline, minocycline, and vancomycin. Our findings reflect the current situation on antibiotic resistance among pathogenic bacteria strains circulating at the study sites during food processing. They are an evidence of potential risk of food poisoning. There is a need to undertake the proper containment measures on the part of authorities or policy makers.
Phase-transition-cycle-induced recrystallization of FAPbI3 film in an open environment toward excellent photodetectors with high reproducibility
Authors: Wang Meng , Cao Fengren , Meng Linxing , Wang Min , Li Liang 
Published: January 2024

Abstract
Perovskite is an attractive building block for future optoelectronic applications. However, the strict fabrication conditions of perovskite devices impede the transformation of lab techniques into commercial applications. Here, a facile annealing-free posttreatment is proposed to reconstruct the perovskite film to obtain high-performance photodetectors with an optimized production rate. With posttreatment by methylamine thiocyanate, the prefabricated formamidinium-lead triiodide (FAPbI3) film will undergo a recrystallization process consisting of a repeating phase-transition-cycle between the black and yellow phases of FAPbI3, which improves the crystal quality and eliminates defects. As a result, some casually prepared or even decomposed perovskite films can be reconstructed, and the dispersion degree of the device performance based on the posttreatment method decreases by approximately 21% compared to the traditional antisolvent method. This facile and annealing-free posttreatment will be an attractive method for the future industrial production of perovskite devices.
Signal Processing and Machine Learning Techniques Based Hybrid Approaches for Decent Fault Classification of Induction Motor
Authors: PANIGRAHY PARTH , CHATTOPADHYAY PARAMITA 
Published: January 2024

Abstract
Learning of better feature representation instinctively by Convolutional Neural Networks (CNN) has inspired to address the unsolved issues in the stator current based multi-class fault diagnosis of induction motor drives. Current envelope of stator current acquired using the Hilbert transform is proven to be the effective pre-processing method to handle the complex data pattern of motor current and reveal the masked defect information. The self-synthesized quality features through deep convolution layers outperforms and reaches an unmatched accuracy level compared to the counterpart-feature engineering scheme. The method of feature engineering is also developed with DHT-DWT based feature extraction process with novel idea of suitable mother wavelet selection scheme. The most notable achievement of this research work is to address the unique advantages of hybridization of signal processing technique and CNN model where the enrichment in feature quality is acquired due to unveiling the buried fault information close to dominating supply frequency. The proposed method is reliable in analyzing multi-class motor fault detection having a good generalization approach. The compact design of hybrid CNN-envelope approach dealt with very low resolution stator current sampled at 1.28 kHz, has reduced the computation intricacies to a great extent and projected it as the right aspirant for real-time applications.
A random-bound Chebyshev method for uncertainty propagation of nonlinear dynamics under imprecise probabilities
Authors: Zhang Licong , Li Chunna , Su Hua , Wang Xiaowei , Yang Sizhi , Gong Chunlin 
Published: January 2024

Abstract
The distribution-free P-box is an effective quantification model for uncertainties with only imprecise probabilistic information. However, its application to nonlinear dynamical systems is limited due to a lack of efficient uncertainty propagation (UP) methods. To end this, this work develops a random-bound Chebyshev (RBC) UP method based on the framework of the interval Monte Carlo (IMC) method. First, the Chebyshev method is applied to solve the interval analysis in the IMC simulations. Here, the bounds of intervals can be regarded as random variables whose cumulative density functions (CDFs) are the CDF bounds of the P-box. Since the CDF bounds of distribution-free P-boxes are always arbitrary and non-parameterized, the data-driven polynomial chaos expansion (DD-PCE), which only requires the information of statistical moments, is introduced to solve the problem of random bounds. Then a sparse-regression strategy is utilized to deal with the ‘curse of dimensionality’ of the DD-PCE for high-dimensional problems. As a result, the RBC method efficiently achieves a non-intrusive UP of nonlinear dynamics with distribution-free P-boxes. The method is also effective for hybrid UP problems with random, interval, and P-box variables. Then the RBC method is validated based on test cases, including a duffing oscillator, a vehicle ride, and an engineering application of launch-vehicle trajectory. The results verify the ability of the method to deal with complex black-box problems. In comparison with the reference solutions based on the IMC simulations, with relative errors of less than 1%, the proposed method requires less than 0.0004% sample size and 0.015% calculation time.