bluuebunny/crossref_metadata_2025_split huggingface dataset:
Amwaprin is a honeybee sperm-binding protein that inhibits sperm motility and enhances sperm viability
Authors: Kim Yun Hui , Kim Bo Yeon , Yoon Hyung Joo , Choi Yong Soo , Lee Kwang Sik , Jin Byung Rae 
Published: December 2023

Abstract
Mated queen honeybees store viable sperm in their spermathecae for egg fertilization throughout their lifetimes, which can span several years. However, the mechanism by which queen honeybees store sperm for extended periods has remained a longstanding question. The molecular basis for the long-term survival of stored sperm in queen honeybees is linked to minimized energy metabolism and reduced production of reactive oxygen species (ROS), but the specific factor responsible for such changes remains unknown. In this study, we present the first evidence of the presence of Amwaprin, a sperm-binding protein, in the seminal and spermathecal fluids of honeybees. We observed that Amwaprin acted as a sperm-binding protein primarily expressed in the testes and spermatheca of honeybees. A lack of binding between Amwaprin and the sperm surface resulted in altered sperm morphology, increased sperm motility, elevated H2O2 production, and decreased sperm viability. Our data demonstrate that Amwaprin inhibits sperm motility and enhances sperm viability. These findings suggest that the reduced sperm motility caused by Amwaprin binding in queen bees’ spermathecae contributes to the long-term survival of stored sperm by minimizing energy metabolism and ROS production.
Various aspects of approximative $\tau$-compactness in Banach spaces
Authors: Das Syamantak , Paul Tanmoy 
Published: December 2023

Abstract
We investigate the notion of approximative τ-compactness in Banach spaces, where τ is the norm or the weak topology. The approximative τ-compactness of Banach spaces is characterized in several ways when the subspace is of finite codimensional. A separable Banach space $X$ is shown to be Asplund if and only if X* admits a dual $CLUR$ renorming. This property is discussed in the context of quotient spaces. We prove that, under certain conditions, the property of approximative τ-compactness passes through M-ideals. Stability results for approximative τ-compactness in the spaces of Bochner integrable functions are also presented.
Exercise Level and Its Related Factors in Patients with Colorectal Cancer Comorbid Diabetes Mellitus undergoing Chemotherapy
Authors: Liu Huan , Zhao Huihua , Yang Zhuojun , Gu Yi , Zhang Wen , Zhou Jiao , Yang Yang , Li Haiyan , Zhu Renmin 
Published: December 2023

Abstract
Aim: To investigate the status quo of exercise management as well as its related factors among patients with colorectal cancer comorbid diabetes mellitus to provide reference for clinical exercise intervention programs.
Methods: A cross-sectional study of 124 patients with colorectal cancer comorbid diabetes mellitus who were undergoing chemotherapy in Shanghai from December, 2022 to April, 2023, was undertaken. A self-made questionnaire was used to investigate the exercise management and its related factors, and multiple linear regression analysis was used to explore the potential related factors.
Results: The mean amount of exercise per week was 3.70±3.50 MET-h/week. The stepwise regression results revealed that exercise level was positively correlated with motor function (r = 0.305, p = 0.001), self-efficacy (r = 0.582, p = 0.000), social support (r = 0.434, p = 0.000), and health behavior change techniques (r = 0.419, p = 0.000).
Conclusion: The patients with colorectal cancer comorbid diabetes mellitus have a low level of exercise, and the level of exercise can be increased by improving their self-efficacy, increasing their social support, and reducing the adverse reactions of chemotherapy.
Changes in High Resolution peripheral Quantitative Computed Tomography (HR-pQCT) in teenagers undergoing a first renal transplantation between baseline and 6 months
Authors: de Mul Aurelie , Leclerc Anne-Laure Sellier , Ginhoux Tiphanie , Levi Charlène , confavreux Cyrille , Aurelle Manon , Portefaix Aurélie , Bacchetta Justine 
Published: December 2023

Abstract
Background
Mineral bone disorders associated to chronic kidney disease (CKD-MBD) frequently persist after renal transplantation (R-Tx), being due to pre-existing CKD-MBD, immunosuppressive therapies and post-Tx hypophosphatemia. We aimed to evaluate bone biomarkers and microarchitecture at the time of and 6 months after R-Tx.
Methods
Here we present the single-center sub-group of patients included between 10 and 18 years of age from the prospective TRANSOS study (NCT02729142). Patients undergoing a first R-Tx were matched on a 1:2 basis on gender, puberty and age with healthy controls (HC) from the VITADOS cohort. Results are presented as median (Q1-Q3). Non-parametric tests were performed.
Results
At a median age of 15 (13-16) years, 19 patients (6 girls, 7 pre-emptive R-Tx, 7 steroid-sparing immunosuppressive strategies) underwent a first R-Tx, with PTH levels of 1.9 (1.4-2.9) the upper limit of normal (ULN).
When comparing HC and patients at the time of R-Tx, bone parameters assessed with High Resolution peripheral Quantitative Computed Tomography (HR-pQCT) were significantly better in patients. Six months after R-Tx, patients displayed significantly impaired trabecular parameters at radius compared to baseline; results were not different at the weight-bearing tibia, neither cortical parameters at both sides.
Six months after R-Tx, 6 (31%) patients displayed acidosis, 10 (53%) persistent hyperparathyroidism (always < 2 ULN) and 5 (26%) increased FGF23 levels; 11 (58%) received phosphate supplementation.
Conclusion
Data on bone health in teenagers receiving a first R-Tx seem reassuring, but radial trabecular bone microarchitecture impairment observed 6 months post-Tx may reflect subtle albeit present post-Tx CKD-MBD.
Congenital Diaphragmatic Hernia: automatic lung and liver MRI segmentation with nnU-Net, reproducibility of pyradiomics features, and a Machine Learning application for the classification of liver herniation.
Authors: Conte Luana , Amodeo Ilaria , De Nunzio Giorgio , Raffaeli Genny , Borzani Irene , Persico Nicola , Griggio Alice , Como Giuseppe , Cascio Donato , Colnaghi Mariarosa , Mosca Fabio , Cavallaro Giacomo 
Published: December 2023

Abstract
Purpose
Prenatal assessment of lung size and liver position is essential to stratify Congenital Diaphragmatic Hernia (CDH) fetuses in risk categories, guiding counseling and patient management. Manual segmentation on fetal MRI provides a quantitative estimation of total lung volume and liver herniation. However, it is time-consuming and operator-dependent.
Methods
In this study, we utilized a publicly available Deep Learning (DL) segmentation system (nnU-Net) for automatic contouring of CDH-affected fetal lungs and liver on MRI sections. Reproducibility was assessed calculating the Jaccard coefficient for manual and automatic segmentation. Pyradiomics standard features were then extracted from both manually and automatically segmented regions. Features reproducibility between the two groups was evaluated through the Wilcoxon rank-sum test and Intraclass Correlation Coefficients (ICCs). We finally tested the reliability of the automatic-segmentation approach by building a ML classifier system for the prediction of liver herniation, based on Support Vector Machines (SVM) and trained on shape features computed both in the manual and nnU-Net-segmented organs.
Results
We compared the area under the classifier Receiver Operating Characteristics curve (AUC) in the two cases. Pyradiomics features calculated in the manual ROIs were partly reproducible by the same features calculated in nnU-Net segmented ROIs and, when used in the ML procedure to predict liver herniation (both AUC around 0.85).
Conclusions
Our results suggest that automatic MRI segmentation is feasible, with good reproducibility of pyradiomics features, and that a ML system for liver herniation prediction offers good reliability.
Trial registration URL:
https://clinicaltrials.gov/ct2/show/NCT04609163?term=NCT04609163&draw=2&rank=1
Clinical Trial Identification n° NCT04609163
Reduced frequency dosing of osimertinib in EGFR-mutant non-small cell lung carcinoma: Real world data
Authors: Noronha Vanita , Sahu Harsh , Kapoor Akhil , Patil Vijay M. , Menon Nandini , Shah Minit , M Dilan Davis , Roy Rumeli , Vivek Srigadha , Janu Amit , Kaushal Rajiv , Prabhash Kumar 
Published: December 2023

Abstract
Introduction
Osimertinib has better efficacy than first generation EGFR-directed TKIs with similar safety profile. However, osimertinib is not affordable for most in developing nations. Moreover, minimum biologically effective dose of osimertinib may be less than approved, given uncertainty surrounding dose determination strategy for targeted agents
Materials and Methods
This was retrospective observational multicentric study aimed to describe the objective response rate(ORR), disease control rate(DCR), progression free survival(PFS), overall survival(OS), and toxicity of osimertinib 80 mg orally administered less frequently than daily (ranging from alternate day to once weekly) in patients with EGFR-mutated non-small cell lung cancer.
Results
Between January 2021 and August 2023, we enrolled 22patients. Six received osimertinib 80mg once weekly, nine received 80mg every three days, and seven received 80mg alternate days. Responses included 0 complete responses, 7(31.8%) partial responses, 9(40.9%) stable diseases, and 5(22.7%) progressive disease. ORR was 31.8%, and DCR was 72.7%. Median PFS was 9.2months (95% confidence interval[CI] 2.9–15.7), and median OS was 17.8months (95%CI, 3.2–32.6). In patients who received reduced frequency osimertinib in the second line and beyond, the ORR was 29.4%, DCR was 70.5%, median PFS was 5.9months (95%CI, 1.1–10.6) and median OS was 17.6months (95%CI, 2.9–32.2). Grade- 3 and higher toxicities were noted in 8(36.3%) patients.
Conclusion
Less frequent dosing of osimertinib may be valid treatment option especially in second line and beyond in patients who cannot afford full dose osimertinib. This may provide additional treatment option with similar toxicity profile as standard dose osimertinib.
MAP3K1 Identified as a Prognostic Biomarker in Breast Cancer After Multi-omics Bioinformatics Analysis
Authors: Narang Binish 
Published: December 2023

Abstract
Background
Despite significant advances in cancer research, cancer remains a major public health concern, with breast cancer being one of the leading causes of death among women. The mitogen-activated protein kinase kinase kinase 1 (MAP3K1) codes for a serine/threonine kinase abundant in the c-Jun N-terminal kinase, mitogen-activated protein kinase, and Nf-kappa-β pathways, which are involved in tumorigenesis.
Methods
Multi-omics bioinformatics analysis on the TCGA and METABRIC datasets from cBioPortal was conducted to analyze MAP3K1’s relevance in breast cancer. Other tools, including TIMER 2.0, Kaplan-Meier Plotter, UALCAN, and STRING, were implemented to provide additional insight into MAP3K1 in different types of omics data.
Results
Results revealed that, though MAP3K1 alterations are relatively uncommon overall, they are most common in breast cancer. These alterations mostly included truncating mutations and often co-occurred with alterations in PIK3CA, an already established biomarker in breast cancer research. Survival analysis indicated that MAP3K1 underexpression was strongly associated with lower patient survival. MAP3K1 was underexpressed for African Americans, triple-negative breast cancer patients, and stage 4 patients, while its phosphoprotein was overexpressed for these demographics.
Conclusions
Drug targets or other targeted therapy options that limit MAP3K1 phosphoprotein expression could potentially improve patient outcomes, especially for the aforementioned demographics. However, limited information is known about this phosphoprotein, so there is an unmet need to address this lack of knowledge and eventually find ways to combat its excessive expression in breast cancer.
The incidence and clinical characteristics of COVID-19 in children with IBD during the Omicron Wave: a single-center experience in China
Authors: Li Rui , Chen Pei-Yu , Li Hui-Wen , Ren Lu , Cheng Yang , Liu Li-Ying , Wei Li-Juan , Zeng Zi-Huan , Xu Wan-Fu , Gong Si-Tang , Geng Lan-Lan 
Published: December 2023

Abstract

Aims
The pandemic of Coronavirus disease 2019 (COVID-19) had a major impact on the health of people worldwide, including the pediatric inflammatory bowel diseases (PIBD) patients. As no study has investigated the susceptibility and disease course of COVID-19 in PIBD patients after the end of zero-COVID policy in China, we conducted a retrospective cross-sectional study in our center.
Methods
A cross-sectional survey enrolling PIBD patients has been completed by online survey, phone, and face-to-face assessment. The demographic data, epidemiological characteristics, clinical manifestations, treatment and prognosis of the patients were analyzed.
Results
PIBD patients represented 55.45%(56/101) of SARS-CoV-2 positive cases between December 1st 2022 and January 31th 2023, 67.86% were male, mean age was 11.15 ± 3.92 years old. Among the SARS-CoV-2 positive cases, 3 patients (5.36%) were asymptomatic, 53 patients (94.64%) had mild symptoms. The main symptoms were fever (92.86%), cough (69.64%), nasal congestion or runnig nose (35.71%), and sore throat (33.93%). No severe case or deaths were reported. All patients recovered from COVID-19 symptoms within one week. We did not observe an association between different clinical disease activities and SARS-CoV-2 infection rates, so as it in different treatments. 51 patients were reported to be in close contact with persons confirmed with COVID-19 infection, and out of them, 36 patients test positive of SARS-CoV-2, which is significantly higher than it in patients without exposure of COVID-19 (70.59% vs. 33.33%, p = 0.002). A total of 10 patients were underweight, of which 9 patients tested positive for COVID-19(p = 0.048). Meanwhile, unvaccinated patients were also found to be more susceptible to SARS-CoV-2 than vaccinated patients(70.97% vs. 48.48%, p = 0.049).
Conclusions
The incidence of SARS-CoV-2 infection in our center of pediatric IBD patients during the Omicron pandemic was 55.45%. No severity or death case was observed. Patients with underweight, unvaccinated and history of exposure to COVID-19 were at higher risk of SARS-CoV-2 infection.
Relationship between chronic rhinosinusitis and risk of obstructive sleep apnea: A nationwide, population-based study
Authors: Cha Hyunkyung , Kim DoHyeon , Lee Hyeon Woo , Lee Yeongrok , Baek Byoung-Joon , Lee Jae Yong , Choi Ji Ho 
Published: December 2023

Abstract

This study aimed to assess relationship between OSA risks, as assessed by STOP-Bang questionnaire, and CRS symptoms. This is a cross-sectional study based on the Korean National Health and Nutrition Examination Survey (KNHANES). In a low-risk group according to the STOP-Bang questionnaire, 3.1% of subjects were CRS patients. However, a gradual increasing trend was observed among different risk groups. In the higher risk group, CRS patients accounted for 5.3% (P < 0.001). This finding was associated with increased occurrences of snoring, tiredness, and apnea (P < 0.001, P < 0.001, and P = 0.016, respectively). Among the four main symptoms of CRS (nasal obstruction, nasal discharge, facial pain/pressure, and decreased sense of smell), nasal obstruction increased from 4.1% in the low-risk group to 7.3% in the high-risk group (P < 0.001). Patients with a decreased sense of smell accounted for 1.9% in the low-risk group and 3.3% in the high-risk group, demonstrating an association of a decreased sense of smell with an increased risk of OSA (P < 0.001). This study found that the proportion of patients with CRS was significantly higher in the group at a higher risk of OSA. Among symptoms of CRS, nasal obstruction and anosmia were found to be associated with an increased risk of OSA.
Identification and Molecular Characterization of NS1 and NS2 Genes of Dengue Virus from two mini outbreaks (2022-2023) in Pakistan
Authors: Kanwal¹ Nida , Batool¹ Anum , Aziz Sadia , Amraiz Amraiz , Akram Madiha , Karim Aman , Ali Liaqat 
Published: December 2023

Abstract
Background
Dengue viral infection is a global threat and approximately 400 million infections and 22000 deaths occur due to this virus worldwide annually. The exact mechanism of dengue virus pathogenesis remains unclear due to the absence of suitable in vivo and in vitro models. So far no antiviral medications available for the clinical treatment of Dengue virus (DENV) infection. The non-structural genes that are involved in disease pathogenesis are still not well defined. For that reason, there is a dire need to characterize these non-structural genes for further functional characterization. The main goal of this study is to identify DENV serotypes circulating in Pakistan during the year 2022 to 2023, characterize the non-structural (NS1 and NS2) genes for identification of their sequence variations and further functional analysis.
Methods
In this study, NS1 positive dengue serum samples were collected from the different molecular diagnostic laboratories and hospitals, in Pakistan. Viral RNA was purified and synthesized complementary DNA (cDNA). Serotype specific primers were used for the identification of dengue virus serotypes (DENV 1–4). Through nested PCR, inner and outer sense primers were used to amplify NS1 and NS2 genes of DENV and confirmed through gel electrophoresis. All purified DNA products were processed for sequencing. For cloning these fragments were then cloned in the TA cloning vector (pcDNA 2.1) and expressed expression vector (pcDNA3.)
Results
In our study, it was confirmed that the most prevalent serotype of DENV in Pakistan is Serotype-2 during the year 2022-23 and also amplified NS1 and NS2 genes. These amplified products were confirmed through Sanger sequencing. Through phylogenetic analysis of these sequences, it was confirmed that the isolated strains are closely related to the strains from Swat, Jeddah, India etc with homology about 97%. The amplified NS1 and NS2A gene fragments were then cloned in the TA cloning vector (pcDNA 2.1) and expressed in mammalian expression vector (pcDNA3.1) for further functional characterization.
Conclusion
Our study has shed light on the most prevalent serotype between the year 2022 to 2023 and characterization on the molecular genetic landscape of non-structural genes (NS1 and NS2). This study will give us a prime way for in-depth investigation of non-structural genes in the dengue virus, contributing valuable insights for the development of antiviral therapy against DENV infection.
The Assesment of Fatigue and Sleep Quality Among Children and Adolescents with Familial Mediterranean Fever: A Case-Control and Correlation Study
Authors: İncesu Çağla , Kayaalp Gülşah Kavrul , Demirkan Fatma Gül , Turan Oya Köker , Çakmak Figen , Akgün Özlem , Ayaz Nuray Aktay , Ömeroğlu Rukiye Nurten 
Published: December 2023

Abstract
Purpose: To evaluate the sleep quality and fatigue levels in children with familial Mediterranean fever (FMF) in comparison to healthy children.
Methods: The Pediatric Quality of Life Multidimensional Fatigue Scale (PedsQL-MFS) and the Pittsburgh Sleep Quality Index (PSQI) and were the instruments utilized to assess fatigue and sleep quality in children with FMF and controls, respectively. Spearman's rank coefficient was decisive in determining the association between patient-reported outcome measures and disease-related features.
Results: Two hundred twenty five (59.3% female) patients and 182 (51.6% female) healthy counterparts were enrolled in the study. In PSQI, where high scores indicate sleep disturbance, the median score was significantly higher in the patient group (5; 3-6), than the control group (3; 2-4) (p <0.001). PEDsQL-MFS demonstrated significantly lower fatigue levels in the control group than patients (p=0.01). The level of fatigue in the patient group was found to increase in correlation with sleep problems (r: -0.750, p< 0.001). Additionally, a high correlation was present between the PSQI/PedsQL-MFS scores and the number of attacks in the last year (r: -0.645, p< 0.001/ r: 0.721, p< 0.001, respectively). There was no difference in terms of fatigue and sleep disorders between mutations (homozygous, heterozygous, or compound heterozygous) in the MEFV gene (p> 0.05).
Conclusions: High disease activity has significant negative impact on the sleep quality and fatigue levels of patients with FMF. This study emphasizes the importance of assessing fatigue and sleep quality with objective outcome tools periodically in FMF patients throughout the disease course.
Wideband CPW Fed Four Port MIMO Antenna-based Microwave Sensor for Deep Brain Temperature in 5G WLAN Bands
Authors: SINGH TILAKDHARI , Mishra Piyush Kumar , Pal Aditya , Tripathi Vijay Shanker 
Published: December 2023

Abstract
This study presents a wideband CPW-fed four-port multi-input multi-output (MIMO) antenna-based sensor for measuring deep brain temperature inside the human head in 5G wireless local area network (WLAN) bands. The range of desired frequency is from 5.45 to 8.56 GHz. The proposed MIMO sensors cover the 5.8 ISM (industrial scientific and medical) band, IEEE 802.11ax-defined Wi-Fi 6E band (6 GHz), IEEE 802.11p WLAN band (5.9GHz), and WPAN band (7.4GHz). A Coplanar Waveguide (CPW) fed approach on a low-cost FR4 substrate is used in designing the planar antenna. In the operational frequency range, the obtained diversity performance parameters show an envelope correlation coefficient (ECC) of very low value (< 0.05), DG of about 10 (> 9.95), total active reflection coefficient (TARC) less than − 13 dB and channel capacity loss (CCL) about less than 0.125 bits/S/Hz. For deep brain temperature measurements, the proposed sensors provide an electric field of 3.92 v/m and a specific absorption rate (SAR) value of 0.16 w/kg at the 9 mm distance inside the human head Phantom. The proposed sensor is capable of measuring 0.5 0C change in temperature at 10 W of input power for 300 seconds when all four ports are active.
New 4-point bending test protocol for the strength grading of timber: relationship between local and global Modulus of Elasticity (MOE) of maritime pine
Authors: LOKO Raoul Spéro ADJOVI , COUREAU Jean-Luc , GALIMARD Philippe 
Published: December 2023

Abstract
This study focuses on the characterization of maritime pine (pinus pinaster) according to standards EN408 and EN384 required for timber grading. A comparison of the EN408 bending test setup is performed with a new 4-point bending protocol, reducing as much as possible, the effects of the shear force in the span where the deflection is measured. The study highlights difficulties to estimate accurately, the elasticity modulus of wood by using EN408 bending test setup, since two approach can be used. This is probably due to inaccuracies in the measurements of small curvatures and due to the local heterogeneities of wood. The EN384 standard anticipates these difficulties by introducing a fitting function, which is not well fitted for maritime pine and probably other species used in timber engineering. An original 4-point bending test is proposed with 75% of the span of the wood beams in pure bending. This configuration clearly exhibits the convergence of measurements, in comparison to EN 408 setup. The deformations generated by this new loading protocol, allow a better evaluation of the bending modulus E0. Moreover, the study reveals that the proposed setup, supplies an increase of elasticity modulus close to 20%. In addition, dynamic tests are also performed in order to characterise the Young's modulus. The experimental results reveal a better agreement between static test, using the new four-point bending configuration and dynamic method, than considering EN408 as a reference.