bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effects of a Bioabsorbable Barrier Membrane Containing Safflower Seed Extracts on Periodontal Healing of 1‐Wall Intrabony Defects in Beagle Dogs
Authors: Song Won‐Seok , Kim Chang‐Sung , Choi Seong‐Ho , Jhon Gil‐Ja , Kim Hak‐Yong , Cho Kyoo‐Sung , Kim Chong‐Kwan , Chai Jung‐Kiu 
Published: March 2005

Background: Recently, there has been much research done into the regenerative potential of materials used in oriental medicine. In several studies, evidence was found that these materials have an effect on bone regeneration. Among these materials, safflower seeds are of particular interest as they have been used for the treatment of blood stasis, bone fracture, and osteoporosis in traditional Korean medicine. In addition, they are known to have anti‐inflammatory effects. The objective of this study is to evaluate the periodontal tissue regenerative effects of a bioabsorbable barrier membrane (polylactide glycolic acid electro‐spun non‐woven membrane) containing safflower seed extracts applied to surgically created 1‐wall intrabony defects in beagle dogs.Methods: One‐wall intrabony defects were surgically created bilaterally at the mesial and distal sides of the mandibular second premolars and mesial side of the fourth premolars. These defects were randomly assigned either to the surgical control group which received a flap operation only or to one of two experimental groups consisting of defects which received a guided tissue regenerative procedure with either a bioabsorbable membrane (PLGA) or a bioabsorbable membrane containing safflower seed extracts (SSE/PLGA). The dogs were sacrificed 8 weeks after the operation, and a comparative histological examination was done.Results: The new cementum formation was 2.49 ± 0.41 mm in the surgical control group, 3.22 ± 0.35 mm in the PLGA group, and 3.67 ± 0.82 mm in the SSE/PLGA group. The extent of new cementum formation in barrier groups was significantly different from the surgical control group (P <0.05). The amount of intrabony cementum was 1.75 ± 0.06 mm, 2.40 ± 0.33 mm, and 2.70 ± 0.81 mm for the surgical control group, the PLGA group, and the SSE/PLGA group, respectively; the amount of infrabony cementum in the barrier groups was significantly different from the surgical control group (P <0.05). The value of the suprabony cementum was 0.73 ± 0.48 mm, 0.82 ± 0.21 mm, and 0.97 ± 0.09 mm for the surgical control group, the PLGA group, and the SSE/PLGA group, respectively, with no significant differences being observed among the treatments. The amount of new alveolar bone formation was 1.74 ± 0.25 mm, 2.36 ± 0.30 mm, and 2.64 ± 0.74 mm for the surgical control group, the PLGA group, and the SSE/PLGA group, respectively, with a significant difference exhibited between the surgical control group and other groups (P <0.05). Superficial root resorption was often observed, but ankylosis was not present.Conclusion: Our results suggest that surgical application of polylactide glycolic acid non‐woven membrane with or without safflower seed extract could promote the regeneration of alveolar bone and cementum in intrabony periodontal defects. J Periodontol 2005;76:22‐33.
Initial Apical Migration of Junctional Epithelium in Rats Following Application of Lipopolysaccharide and Proteases
Authors: Ekuni Daisuke , Tomofuji Takaaki , Yamanaka Reiko , Tachibana Kentaro , Yamamoto Tatsuo , Watanabe Tatsuo 
Published: March 2005

Background: Apical migration of junctional epithelium (JE) occurs in association with periodontal pocket formation. The aim of this study was to investigate the gingival changes occurring during apical migration of the JE following application of factors associated with inflammatory periodontal disease pathogenesis.Methods: Six‐week‐old male Wistar rats were divided into six groups: three experimental groups to investigate gingival changes following 2, 4, and 8 weeks topical application of lipopolysaccharide (LPS) and proteases and three control groups using pyrogen‐free water. After 2, 4 or 8 weeks, nuclear DNA fragmentation was detected in periodontal ligament (PDL) fibroblasts using the terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end‐labeling (TUNEL) method, and proliferative activities of the basal cells and fibroblasts were evaluated through expression of proliferating cell nuclear antigen (PCNA). Collagen destruction was examined histologically.Results: Gingiva treated with LPS and proteases showed an increase in PCNA‐positive basal cells but not the fibroblasts. Collagen destruction was observed at 2 weeks; apical migration of the JE and TUNEL‐positive fibroblasts was seen at 4 weeks.Conclusions: Following application of LPS and proteases to rat gingival sulci, the apical migration of the JE appears to occur simultaneously with the apoptosis of PDL fibroblasts, which in turn follows proliferation of the basal cells and collagen destruction. J Periodontol 2005;76:43‐48.
Modulation of Clinical Expression of Plaque‐Induced Gingivitis: Interleukin‐1 Gene Cluster Polymorphisms
Authors: Scapoli Chiara , Tatakis Dimitris N. , Mamolini Elisabetta , Trombelli Leonardo 
Published: March 2005

Background: The purpose of the present study was to determine the association of interleukin‐1 (IL‐1) gene polymorphisms with clinical parameters of gingivitis in a large experimental gingivitis trial and with each of two subgroups, high responders (HR) and low responders (LR), with distinct susceptibility to gingivitis.Methods: Ninety‐six systemically and periodontally healthy non‐smokers, 46 males (mean age: 23.9 ± 1.7) and 50 females (mean age: 23.3 ± 1.6) were included in a randomized, split‐mouth, localized 21‐day experimental gingivitis trial. Plaque index (PI), gingival index (GI), gingival crevicular fluid volume (GCF), and angulated bleeding score (AngBS) were recorded. Two subgroups were defined from the total study population (HR, LR) characterized by substantially different severity of gingival inflammation despite similar plaque accumulation rate. The study population was typed for interleukin‐1 alpha (IL‐1A+4845), interleukin‐1 beta (IL‐1B+3953, IL‐1B–511), and IL‐1 receptor antagonist (IL‐1RN, intron 2 variable number tandem repeats) gene polymorphisms. Gene variants were analyzed by amplifying the polymorphic region using polymerase chain reaction, followed by restriction‐enzyme digestion and agarose gel electrophoresis.Results: Neither IL‐1A+4845, IL‐1B+3953, or the combined (IL‐ 1A+4845*2 ‐ IL‐1B+3953*2) genotype was associated with clinical parameters in the overall population. IL‐1RN was significantly associated with test quadrant PI (P = 0.046), GCF (P = 0.05), and GI (P = 0.018). The genotype distribution in HR and LR subjects was significantly different for IL‐1RN (P = 0.045) and for IL‐1B–511 (P = 0.023).Conclusion: The results of the present study suggest an association between IL‐1RN polymorphism and subject‐based clinical behavior of the gingiva in response to de novo plaque accumulation, as well as a possible association between IL‐1B–511 polymorphism and gingivitis susceptibility. J Periodontol 2005;76:49‐56.
Coping With Stress: Its Influence on Periodontal Therapy
Authors: Wimmer Gernot , Köhldorfer Gabriele , Mischak Irene , Lorenzoni Martin , Kallus K. Wolfgang 
Published: March 2005

Background: Individual stress coping strategies appear to influence periodontal disease: individuals with inadequate stress behavior may be at greater risk of developing disease. The purpose of this 24‐month prospective study was to examine the influence of different coping behaviors on a non‐surgical periodontal therapy and on the course of periodontal disease.Methods: In 80 patients with chronic periodontitis, a non‐surgical periodontal treatment was conducted after their individual stress coping strategies had been recorded. After 2 years of regular maintenance, their periodontal condition was evaluated. The stress coping questionnaire was used to obtain psychodiagnostic data. Clinical attachment loss (CAL) served as the clinical parameter.Results: Patients with a defensive coping style had statistically significant poorer attachment values (P = 0.000) after 2 years compared to patients with other coping behaviors. The percentage of sites with slight to moderate CAL (<5 mm) was significantly less in patients with a defensive coping style than in patients with other coping strategies (P = 0.000). The number of sites with severe advanced CAL (>5 mm) was significantly correlated with a suppressive coping style (P = 0.0001). None of the individual stress coping styles revealed significant overall changes over time. The subtest of drug use (alcohol, nicotine, tranquilizers) as well as changes in this subtest over time were significantly correlated with the CAL (P = 0.003); an increase in the t value of the subtest of drug use was accompanied by a significant increase in CAL.Conclusions: The results of this study show that passive coping strategies were more pronounced in advanced disease as well as in cases of poor response to a non‐surgical periodontal treatment, whereas patients with active coping modes had milder disease and a more favorable course of treatment. Thus, maladaptive behavior, especially in association with behavior‐related risk factors such as smoking, are of great importance in the medical history, treatment, and maintenance of patients with periodontal disease. J Periodontol 2005;76:90‐98.
Electrostatic Effects on the Thermodynamics of Protonation of Reduced Plastocyanin
Authors: Battistuzzi Gianantonio , Borsari Marco , Di Rocco Giulia , Leonardi Alan , Ranieri Antonio , Sola Marco 
Published: March 2005

AbstractThe L12E, L12K, Q88E, and Q88K variants of spinach plastocyanin have been electrochemically investigated. The effects of insertion of net charges near the metal site on the thermodynamics of protonation and detachment from the copper(I) ion of the His87 ligand have been evaluated. The mutation‐induced changes in transition enthalpy cannot be explained by electrostatic considerations. The existence of enthalpy/entropy (H/S) compensation within the protein series indicates that solvent‐reorganization effects control the differences in transition thermodynamics. Once these compensating contributions are factorized out, the resulting modest differences in transition enthalpies turn out to be those that can be expected on purely electrostatic grounds. Therefore, this work shows that the acid transition in cupredoxins involves a reorganization of the H‐bonding network within the hydration sphere of the molecule in the proximity of the metal center that dominates the observed transition thermodynamics and masks the differences that are due to protein‐based effects.
Enhancement of Chemical Selectivity by Microreactors
Authors: Yoshida J. , Nagaki A. , Iwasaki T. , Suga S. 
Published: March 2005

AbstractThis article provides a brief outline of the state of the art of reactions using microreactors with special emphasis on the enhancement of product selectivity. The efficient micromixing possible with a short diffusion path increases the product selectivity of competitive parallel reactions and competitive consecutive reactions. This concept has been expanded to control of the molecular weight and molecular weight distribution in carbocationic polymerization. The efficient heat transfer, based on high surface to volume ratios, allows precise temperature control and is also effective for the control of highly exothermic reactions, such as free radical polymerization.
Microreaction for Microfuel Processing: Challenges and Prospects
Authors: Shah K. , Ouyang X. , Besser R. S. 
Published: March 2005

AbstractThe processing of high density liquid hydrocarbon fuels appears to be the most promising method of supplying a hydrogen stream for feeding portable fuel cells. Furthermore, microchemical systems are a strong enabling aspect of compact fuel processors due to their advantageous heat and mass transport and inherent compactness. However, a number of crucial challenges exist for the realization of practical fuel processors. In this article, these challenges are addressed, and two examples are shown of how the challenges can be attacked in the context of implementation of microchemical systems. The examples involve (a) appropriate measurement of kinetics in microchannel‐catalyst systems with preferential oxidation of carbon monoxide as a model, and (b) thermal integration of reactor components with a methanol steam reformer as a model.
Photocontrollable Self‐Assembly
Authors: Yagai Shiki , Karatsu Takashi , Kitamura Akihide 
Published: March 2005

AbstractThe incorporation of photoswitching molecules into molecular building blocks creates the possibility of photoresponsive self‐assemblies in which the self‐assembled architecture or self‐assembling process can be controlled by external light stimulus. Among the photoswitching molecules, azobenzene has been used most widely by virtue of the large photoinduced changes in its molecular geometry and physical properties. This article reviews how azobenzene can be effectively used to construct the self‐assemblies in which supramolecular structure and formation/dissociation can be altered by light.
Halteria grandinella: A rapid swimming ciliate with a high frequency of ciliary beating
Authors: Ueyama Sumiko , Katsumaru Hironobu , Suzaki Toshinobu , Nakaoka Yasuo 
Published: March 2005

AbstractA ciliated protozoan, Halteria grandinella, swam backward rapidly with a migration distance per second attaining 100 times the cell size. This high swimming velocity was accompanied by a high frequency of ciliary beating. Recordings with a high‐speed digital video (103 frames/s) revealed that the frequency during forward and backward swimming was, respectively, 105 ± 10 Hz and 260 ± 30 Hz. These frequencies are the highest among cilia and flagella reported to date. Electron microscopic observation of the ciliary structure confirmed normal 9 + 2 arrangements of the axoneme except that cilia for migration are bundled into membranelles. Ciliary beating of saponin‐treated cells was reactivated by the addition of Mg2+‐ATP, although the beating amplitude was smaller than that of intact cells. Kinetic analysis of the ATP‐dependent increase of beating frequency revealed that the maximal frequency in the presence of free Ca2+ and 0.9 μM Ca2+ was approximately 60 and 110 Hz, respectively. A possible mechanism to increase beating frequency with Ca2+ is discussed. Cell. Motil. Cytoskeleton 60:214–221, 2005. © 2005 Wiley‐Liss, Inc.
Characterization of myosin‐II binding to Golgi stacks in vitro
Authors: Fath Karl R. 
Published: March 2005

AbstractIn addition to important roles near the actin‐rich cell cortex, ample evidence indicates that multiple myosins are also involved in membrane movements in the endomembrane system. Nonmuscle myosin‐II has been shown to have roles in anterograde and retrograde trafficking at the Golgi. Myosin‐II is present on Golgi stacks isolated from intestinal epithelial cells and has been localized to the Golgi in several polarized and unpolarized cell lines. An understanding of roles of myosin‐II in Golgi physiology will be facilitated by understanding the molecular arrangement of myosin‐II at the Golgi. Salt‐washing removes endogenous myosin‐II from isolated Golgi and purified brush border myosin‐II can bind in vitro. Brush border myosin‐II binds to a tightly bound Golgi peripheral membrane protein with a K1/2 of 75 nM and binding is saturated at 0.7 pmol myosin/μg Golgi. Binding studies using papain cleavage fragments of brush border myosin‐II show that the 120‐kDa rod domain, but not the head domain, of myosin heavy chain can bind directly to Golgi stacks. The 120‐kDa domain does not bind to Golgi membranes when phosphorylated in vitro with casein kinase‐II. These results suggest that phosphorylation in the rod domain may regulate the binding and/or release of myosin‐II from the Golgi. These data support a model in which myosin‐II is tethered to the Golgi membrane by its tail and actin filaments by its head. Thus, translocation along actin filaments may extend Golgi membrane tubules and/or vesicles away from the Golgi complex. Cell Motil. Cytoskeleton 60:222–235, 2005. © 2005 Wiley‐Liss, Inc.
The IVADo regimen—A pilot study with ifosfamide, vincristine, actinomycin D, and doxorubicin in children with metastatic soft tissue sarcoma
Authors: Bisogno Gianni , Ferrari Andrea , Bergeron Christophe , Scagnellato Angela , Prete Arcangelo , Alaggio Rita , Casanova Michela , D'Angelo Paolo , Di Cataldo Andrea , Carli Modesto 
Published: March 2005

AbstractBACKGROUNDThe role of doxorubicin (Doxo) as part of multidrug regimens used to treat children with soft tissue sarcoma (STS) is controversial. To evaluate the feasibility of combining Doxo with the well established ifosfamide, vincristine, and actinomycin D (IVA) regimen, the Italian STS Committee performed a pilot study on a series of children with metastatic STS.METHODSBetween July 2002 and February 2004, 29 evaluable patients were enrolled in this study; 19 patients had rhabdomyosarcoma, 5 patients had peripheral neuroectodermal tumor, and 5 patients had other types of STS. The IVA‐Doxo (IVADo) regimen included ifosfamide 3 g/m2 on Days 1 and 2, vincristine 1.5 mg/m2 on Day 1, actinomycin D 1.5 mg/m2 on Day 1, and Doxo 30 mg/m2 on Days 1 and 2. Three courses of IVADo were to be administered in the initial part of treatment and analyzed for toxicity and tumor response.RESULTSOverall, 92 cycles were delivered. Major regimen‐related toxicity was myelosuppression, with Grade 4 neutropenia in 67% of cycles and fever and neutropenia in 37% of cycles. Nonhematologic toxicity included Grade 3–4 mucositis (6.5% of cycles), constipation (9.7%), and peripheral neuropathy (6.5%). Other manifestations of major toxicity were venoocclusive disease and seizures, which occurred in one patient each. All but 1 patient with a malignant schwannoma showed some degree of tumor volume reduction; however, considering only complete and partial remissions, the response rate was 76% (± 7.9%).CONCLUSIONSThe intensive IVADo regimen was effective against pediatric STS with acceptable toxicity. This combination will be investigated in high‐risk patients with rhabdomyosarcoma in a randomized trial launched by the European pediatric Soft tissue sarcoma Study Group. Cancer 2005. © 2005 American Cancer Society.
Tissue inhibitor of metalloproteinase 1 is an independent predictor of prognosis in patients with nonsmall cell lung carcinoma who undergo resection with curative intent
Authors: Gouyer Valérie , Conti Massimo , Devos Patrick , Zerimech Farid , Copin Marie‐Christine , Créme Evelyne , Wurtz Alain , Porte Henri , Huet Guillemette 
Published: March 2005

AbstractBACKGROUNDMatrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play a role in the processes of extracellular matrix degradation. Changes in their expression levels have been observed in various tumor types, including lung carcinoma. However, their clinical significance and their prognostic importance in the progression of nonsmall cell lung carcinoma (NSCLC) remain to be specified. In this study, mRNA expression levels of MMP‐1, MMP‐9, TIMP‐1, and TIMP‐2 were evaluated in patients with resected NSCLC, and their associations with disease progression and prognosis were determined.METHODSBetween June 1996 and December 1999, 116 patients underwent resection for NSCLC. Expression levels of MMPs and TIMPs were evaluated using Northern blot analysis in these NSCLC tissue samples and in 39 matched samples of normal lung tissue.RESULTSMMP‐1, MMP‐9, and TIMP‐1 expression levels were increased in tumor samples compared with matched, corresponding normal tissues. In contrast, TIMP‐2 expression was decreased in tumor samples. MMP‐1 tumor expression was correlated significantly with the evolution of lymph node status and tumor‐lymph node‐metastasis (TNM) stage. In contrast, MMP‐9 tumor expression was correlated significantly with increased T stage. TIMP‐1 overexpression was an independent predictor of worse survival in patients with NSCLC that was not associated with other prognosis factors, such as TNM stage.CONCLUSIONSThe overexpression of TIMP‐1 was an independent prognostic marker in patients with NSCLC, and evaluating TIMP‐1 may be important for identifying patients who are at greater risk of disease recurrence. Cancer 2005. © 2005 American Cancer Society.
A basic study of adaptive particle swarm optimization
Authors: Ide Azuma , Yasuda Keiichiro 
Published: March 2005

AbstractThis paper points out that meta‐heuristics should have not only robustness and adaptability to problems with different structure but also adjustability of parameters included in their algorithms. Particle swarm optimization (PSO), whose concept began as a simulation of a simplified social milieu, is known as one of the most powerful optimization methods for solving nonconvex continuous optimization problems. Then, in order to improve adjustability, a new parameter is introduced into PSO on the basis of the proximate optimality principle (POP). In this paper, we propose adaptive PSO and the effectiveness and the feasibility of the proposed approach are demonstrated on simulations using some typical nonconvex optimization problems. © 2005 Wiley Periodicals, Inc. Electr Eng Jpn, 151(3): 41–49, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20077
Measurement of secondary electron emission coefficient of plane target in plasma immersion ion implantation
Authors: Nakamura Keiji , Ando Masaki , Sugai Hideo 
Published: March 2005

AbstractThis paper describes in‐process measurements of secondary electron emission coefficient (SEEC) during plasma immersion ion implantation for a plane target. In the plane target, trajectories of secondary electrons (SEs) emitted from the target strongly depend on the plasma conditions as well as the amplitude of pulse voltages applied to the target because of variation of the curvature radius of the sheath formed around the target. Therefore, absolute measurements of the SE current as well as of the SEEC were difficult to perform. However, calibration of an SE detector using a plane target with very high SEEC enabled the SEEC measurements. The measurements of the SE current revealed that the sheath curvature was affected by the plasma condition and the target shape. This calibration will be useful for SEEC measurements of arbitrary‐shaped target. © 2005 Wiley Periodicals, Inc. Electr Eng Jpn, 151(3): 1–7, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20096
A method for suppressing line overload phenomena using NAS battery systems
Authors: Ohtaka Toshiya , Iwamoto Shinichi 
Published: March 2005

AbstractThis paper considers the superior operating control function and instantaneous discharging characteristics of NAS battery systems, and proposes a method for determining installation planning and operating control schemes of NAS battery systems for suppressing line overload phenomena. In the planning stage, a target contingency is identified, and an optimal allocation and capacity of NAS battery systems and an amount of generation changes are determined for the contingency. In the operation stage, the control strategy of the NAS battery system is determined. Simulations are carried out for verifying the validity of the proposed method using the IEEJ 1‐machine V‐system model and an example 2‐machine 16‐bus system model. © 2005 Wiley Periodicals, Inc. Electr Eng Jpn, 151(3): 19–31, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20102