bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultra-fast eco-friendly UHPLC–MS/MS methodology for the quantification of ASP3026 in human liver microsomes: Evaluation of metabolic stability via in silico software and in vitro metabolic incubation
Authors: Attwa Mohamed W. , Abdelhameed Ali S. , Kadi Adnan A. 
Published: February 2025

AbstractASP3026 is a recently formulated and highly selective inhibitor designed to target the ALK kinase. ASP3026 efficiently inhibited ALK kinase activity and demonstrated superior selectivity at a panel of Tyr-kinases compared to crizotinib. The target of this investigation was to establish a highly accurate, fast, green, and highly sensitive Ultra-high performance liquid chromatography- Tandem mass spectrometry (UHPLC-MS/MS) technique for assessing the concentration of ASP3026 in human liver microsomes (HLMs). In vitro incubation, the metabolic stability of ASP3026 in HLMs was evaluated using this known approach. The validation steps for the UHPLC-MS/MS analytical technique in the HLMs were performed along with the bio-analytical method validation guidelines settled by the US-FDA. To increase the ecological sustainability of the current UHPLC-MS/MS system, a lower flow rate of 0.3 mL min−1, a shorter elution duration of 1 min, and a reduced consumption of ACN have been implemented. A screening of the chemical structure of ASP3026 for hazardous alerts and metabolic lability was performed by the StarDrop package, that includes the DEREK and P450 modules. The analytical separation of ASP3026 and fenebrutinib (FNB) on the reversed phase Eclipse Plus C18 column was performed using an isocratic mobile phase approach. The calibration curve produced by the ASP3026 showed a linear association over the level range of 1–3,000 ng mL−1. A study was conducted to evaluate the precision and accuracy of UHPLC-MS/MS technology in evaluating both intra-day and inter-day variations. The accuracy exhibited a range of −1.56%–7.33% across various days, and a range of −0.78%–10.66% within the same day. The ASP3026 underwent in vitro half-life and intrinsic clearance measurements, yielding values of 14.32 min and 56.62 mL min−1 kg−1, correspondingly. According to in silico software research, using minor modifications to the piperazine component or substituting the group in drug design has the potential to improve the metabolic safety and stability of novel derivatives in comparison to ASP3026.
Public priorities in psychedelics research
Authors: Bălăeţ Maria 
Published: February 2025

AbstractThis commentary explores public priorities in psychedelic research, drawing insights from extensive conversations between the author and the general public during lectures and seminars across the UK. Two primary themes emerged from this exchange: areas where further research is desired and concerns about the current direction of psychedelic research. Key topics of interest include psychedelics' effects on neurodivergent individuals, ageing, pain management, cognitive enhancement, psychosis, and interactions with the female menstrual cycle. Areas of concern pertain to the involvement of pharmaceutical companies in psychedelics research, the potential medicalisation of psychedelics, and the growing interest in psychedelic tourism. The commentary emphasises the need for a constant dialogue between scientists and the public, critical engagement with research and the integration of diverse perspectives in order to foster a broader understanding of psychedelics and their potential applications in this rapidly evolving field.
"The pen is a powerful weapon; it can make you change": Exploring empowerment through reflective writing in adolescents
Authors: Wegner Lisa , Struthers Patricia , Mohamed Suraya 
Published: February 2025

Introduction: Adolescent programmes have been criticised for implementing activities but not allowing opportunities for reflection. One way that reflection can be facilitated is through writing. This paper describes the use of reflective writing with adolescents as part of a health promoting schools project in Cape Town, South Africa.Method: The project included an annual leadership camp for adolescent learners. A descriptive, qualitative study was conducted to describe how the learners responded to writing as a means to express their feelings about, and experiences of, the camp. Thirty learners participated in two writing sessions resulting in 53 written reflections that were analysed thematically.Findings: Three themes emerged: (1) Reflections on self; (2) Reflections on self in relation to others; and (3) Reflections on writing. Participants reflected on connecting with complex, positive and negative feelings, and felt that reflective writing had enabled them to express themselves with courage and honesty, connect with themselves, identify weaknesses and let go of negative emotions and feelings. Furthermore, writing was perceived as being non-judgmental, providing a sense of relief and release, and evoking emotions.Â Conclusion: Reflective writing enabled the participants to acknowledge personal changes and development, and provided insight into their feelings and experiences. This study shows the value of reflective writing for use by health professionals such as occupational therapists, as a tool for empowerment in the pursuit of health and wellbeing. Â Key words: adolescents, empowerment, health promoting schools, reflective writing
Current concepts in the management of infected non-union of the femur: internal versus external fixation
Authors: Kaul R. , Dubey R. R. , Agrawal R. , Pande A. 
Published: February 2025

Introduction The management of infected non-unions continues to be a herculean task for the orthopaedic surgeon due to the emergence of microbial resistance, failure of fixation, frequent re-fractures and resurgence of previously treated infection.The aim of the paper was to structure the approach to the management of patients with infected femoral non‑union based on the literature review and surgeons’ experience.Material and methods A detailed literature review, including current updates on the management of fracture‑resistant infections (FRI) and non-union of the femur was conducted. Search words and phrases used for navigation in the international medical literature platforms were: osteomyelitis, non-union, diagnostic solutions, local antibiotics, biomechanical stability.Results and Discussion The principles of surgical management of infected non-union of the femur remain the same: (a) adequate soft tissue sampling; (b) thorough debridement; (c) fracture stabilization; (d) dead space and defect management; (e) delivery of local antibiotics and (f) soft tissue coverage. The goal of surgery is to get rid of infection. There is no place for empirical treatment of suspected infection. Therapy should be initiated based upon microbial cultures of deep tissue specimens. While selecting the type of hardware for non-union of the femur, one often encounters a dilemma concerning the most appropriate surgical tool for stabilization. Internal fixation with bone grafting would depend on the size of the gap; commonly defects < 6 cm are treated with this modality. External fixation becomes indispensable in certain scenarios such as poor local skin and soft tissue conditions, associated limb length discrepancy > 2 cm, large defect gaps ≥ 6 cm, concomitant deformity, small fragments or osteopenic bone.Conclusion Based on this review of current concepts, the authors conclude that there is no ideal or universal approach for management of infected non-union of the femur, and the approach may vary depending on the technical expertise available and the institutional practices. Irrespective of the modality used, the golden rules of fixation remain the same, alignment, preservation of biology, contact of fragments, stability and early restoration of function.
Impact of personalized alignment technique on implant components position in total knee arthroplasty
Authors: Kuzin V. V. , Kuzin A. V. , Germanov A. V. , Shpak M. A. 
Published: February 2025

Introduction Due to substantial rates of dissatisfaction in patients with mechanical alignment in total knee replacement, surgeons began searching for alternative techniques to improve functional outcome. In the recent decade, kinematic alignment that is not based on the mechanical axis of the femur has become the most popular alternative to mechanical alignment. Kinematic alignment technique development has led to creation of a personalized alignment technique.Purpose To compare postoperative implant positions in full-length standing lower-leg radiographs between kinematic alignment and mechanical alignment groups of patients.Materials and methods A prospective, single-center, randomized, controlled study was performed in 139 patients with grade 3–4 knee osteoarthritis (Kellgren – Lawrence). We collected data from 76 cases of mechanical alignment (66 women and 10 men) and 83 cases of personalized alignment group (60 women and 23 men). There were no patients with significant post-traumatic or other deformities of the lower limb which can alter the results in the study. All measurements were done on digital full-length standing X-rays of the lower legs with special MediCAD software.Results The positions of the implant components in mechanical and personalized alignments did not differ significantly in many parameters after operations, despite the fact that the alignment was based on completely different principles. There were no differences between the average values of the angles after operations with mechanical and anatomical axes of the femur in both study groups (the difference was 0.1° at p = 0.595). The only difference in the groups was the position of the tibial component in relation to the horizontal surface in the standing position: in personalized alignment, the angle was 0.9°, and in mechanical alignment it was 2.4° valgus (p < 0.001).Discussion The absence of significant difference in the postoperative leg alignment and implant position except in the joint line orientation between the groups demonstrates possibility to achieve good leg alignment with both techniques. In the personalized alignment group, the joint line orientation in the coronal plane was found nearly parallel to the ground which can result in a more balanced weight distribution compared to mechanical alignment.Conclusion In patients who receive total knee replacement with the personalized technique, the postoperative lower limb alignment was found within the safe boundaries of 3° from the mechanical axis while the joint line orientation in the coronal plane was significantly closer to be parallel with the ground compared with mechanical alignment group.
Synthesis and Characterization of Ca, Mg, Fe and Zn Nanoparticles and its Impact on Bacillus thuringiensis
Authors: Gayathri M. , Venkateswarlu N.C. , Prasad T. N.V.K.V. 
Published: February 2025

Background: Bacillus thuringiensis (Bt) is widely used to manage several lepidopteran insect pests including tobacco caterpillar, Spodoptera litura in groundnut. Nanoparticles (NPs) can make agricultural inputs like fertilizers and pesticides more effective and efficient and have shown the ability to enhance the insecticidal action of Bt when combined. They may increase the susceptibility of target insects to Bt toxins by damaging insect gut cells or interfering with their immune responses to develop nanostructured catalysts and to increase the Cfu/g with Bt. The current study is aimed to assess the impact NPs of on Bacillus thuringiensis (Bt) mass production under in vitro conditions. Methods: The synthesis of Ca, Mg, Fe and Zn nanoparticles was carried out using sol-gel method. Characterization of the nanoparticles was done by using UV-vis spectrum, XRD, Particle size and zeta potential analysis, TEM and FT-IR techniques. These NPs were tested as catalysts in the production of biopesticide Bt. Result: The synthesized and characterized NPs were clearly indicated that the prepared nanoscale oxide materials were highly stable and also been observed with uniform surface morphological features. The highest Colony forming units (Cfu)/gram (g) of Bt (17.3´109. 16.9´109, 16.5´109 and 15.0´109) was recorded in treatments with 50 ppm MgO, 20 ppm CaO, 10 ppm ZnO and 50 ppm FeO (as against 6.7´109 Cfu/g in the control.
Understanding Patient Needs and Perceptions of Doula Care in South Florida
Authors: Marudo Catherine , Nicotra Caroline , Chou Brandon , Amani Jamarah , Antoine Rebekah , Marbin Staci , Sale Ana Carolina G. , Tomlinson Ana , Fletcher Michelle 
Published: February 2025

ABSTRACTRacial and ethnic disparities continue to affect maternal mortality, morbidity, and birth outcomes. Historically marginalized communities currently face higher rates of cesarean surgery, preterm birth, low birth weight, and infant mortality. Increased accessibility to culturally congruent doula services can help bridge disparities in maternal mortality, morbidity, and birth outcomes. This feasibility study aimed to characterize the need for doula care among patients of two large academic hospitals to inform the creation of a hospital-based doula program. In partnership with community-based midwives and doulas, we created an institutional review board-approved, anonymous, cross-sectional survey to consenting patients from two large academic health-care centers, one of which is a large safety-net hospital. Inclusion criteria included patients between the ages of 18 and 49 years who have had previous pregnancies, are currently pregnant, are thinking about becoming pregnant, or are not currently considering pregnancy. Surveys were offered in English, Spanish, and Haitian Kreyol. A total of 176 patients participated in and completed our survey study. Patients with an annual household income between $25,000 and $50,000 (Odds Ratio = 4.87, 95% confidence interval [1.02, 23.36]) were almost five times more likely than those with an annual household income below $25,000 to express interest in doula care. Among patients who expressed interest in doula care, there was a statistically significant association between the ability to pay for doula care and race (X2[8,N= 148] = 19.25,p= .01). Results illustrated an increased need for doula care, especially among patients from low-income and historically marginalized groups. More research is needed to understand why differences among demographic groups exist in our patient population. At the same time, other health-care systems nationwide are further encouraged to evaluate doula support needs within their community.
Methodology for Determining the Criterion for the High-Quality Formation of T-Shaped Joints by Resistance Projection Welding Using Mathematical Modeling
Authors: Yumanau D. N. , Furmanau S. M. , Yumanava H. N. 
Published: February 2025

Resistance projection welding of various materials and alloys has become widespread in various branches of the machine-building industry due to the high productivity of the process, technological and economic parameters of manufacture of manufacture and a wide diversity of types of welded joints. Despite its widespread use, technological processes of resistance projection welding in production conditions are not always able to provide consistently high quality of welded joints. Due to the fact that the calculation of welding operation parameters is carried out according to a universal methodology based on the heat balance equation, there are no recommended parameters for different types of joints, which, as a rule, leads to an overestimation or underestimation of welding current values. Incorrectly defined parameters of projection welding lead to the appearance of a large number of defects in T-shaped joints. Mathematical modeling is one of the effective ways to improve the quality of welded joints obtained by resistance projection welding. Existing mathematical models of the projection welding process, as a rule, have significant approximations in setting important parameters of the operation from the point of view of the welding cycle, therefore, the development of a model that takes them into account is an urgent task. The paper examines the developed method of mathematical modeling of projection welding process of T-shaped joints, taking into account the phase control of the heat input power, which is used to determine the influence of energy mode parameters on displacement characteristics of movable electrode of a contact welding machine. Based on the results of mathematical modeling, recommended dependences of the parameters of resistance projection welding modes of T-shaped joints have been obtained. The model of the resistance projection welding process of T-shaped joints is implemented in the ANSYS software product. The adequacy of the developed mathematical modeling methodology has been confirmed by experimental studies of the resistance projection welding process.
Formation of the Microclimate of Residential Premises during Heating with a Radiant-Convective Heating Device
Authors: Livanskiy D. G. , Holub Y. V. 
Published: February 2025

This article examines the peculiarities of microclimate formation when using radiant-convective heating device - radiator. The main purpose of the work is to determine the advantages and disadvantages of radiator heating in residential premises, to assess the influence of window sizes, room dimensions and the device on the formation of the microclimate in the room. The study includes the analysis of temperature and velocity fields in three representative rooms of different sizes and configurations. The authors identify the disadvantages of current heating systems associated with the uneven temperature distribution across the height of the room and the formation of zones of reduced comfort near the windows. The work examines three typical rooms for which mathematical modeling of thermal processes is carried out using differential equations to describe the processes of heat and mass transfer. The main attention is paid to the influence of window sizes, room dimensions and heating devices on the microclimate. The authors note that radiators do not always cover the entire width of windows, which leads to the formation of cold zones at their edges, the penetration of cold air deeper into the room and the formation of discomfort zones inside the room. The simulation results show that the indoor air parameters comply with GOST standards, however, the subjective comfort of residents may decrease due to uneven temperature distribution. In conclusion, it is concluded that it is necessary to develop new types of heating devices that will more effectively cope with the task of maintaining a comfortable microclimate and will eliminate the negative impact of cold air penetration through window openings on comfort.
Climatic Factors and Their Role in Energy Management and Water Consumption in the Urban Water Supply System
Authors: Kapanski A. A. 
Published: February 2025

The article considers the influence of climatic factors on energy and water consumption of the urban water supply system. The purpose of the study is to identify the relationships between outdoor air temperature, precipitation and load on the water supply system to substantiate the factors necessary for creating seasonal models of water consumption and increаsing the energy efficiency of water intakes. The objective of the scientific work was to test the hypothesis, according to which an increase in air temperature leads to an increase in water consumption and, as a consequence, to an increase in energy costs for the operation of pumping stations and water treatment systems. At the same time, it was assumed that precipitation would have the opposite effect, reducing water consumption during periods of intense rainfall. The work used correlation analysis to assess the strength and direction of the relationships between air temperature, precipitation and energy and water consumption parameters. Regression analysis was used to quantitatively assess the impact of climatic factors. The moving average method was used to smooth the data and reduce the spread of random fluctuations. Data filtering and cutting methods were also used, which made it possible to divide them by temperature thresholds and conduct separate studies for different ranges. The obtained results demonstrate that the growth of energy consumption is closely related to the increase in water demand, which increases with each degree of outside air temperature. This is explained by the expected increase in water consumption for irrigation and household needs during warm periods. Above 25 °C, the temperature factor determined 15.8 % of water consumption, which also coincided with the growth of this contribution to the electricity consumption of the system (15.6 %). However, when analyzing data in the area of negative temperatures, no obvious relationship was observed between temperature and water demand. At the same time, a correlation was found between temperature and electricity consumption, which is associated with additional costs for maintaining the water supply system in cold climates. Conclusions are made about the need for further study of additional variables, such as types of weekdays (working days, weekends, holidays), seasons of the year, as well as other socio-economic factors affecting water consumption and energy costs. The comprehensive results of the work can be used for planning the work of water utilities, managing energy resources and developing strategies to improve energy efficiency in water supply systems.
Short-Radius Multi-Lateral Well Construction by Novel Directional Steel Shot Drilling: Cost-Effective Solution for Geothermal Production Enhancement
Authors: Reinicke A. , Zikovic V. , Bergen F. V. , Blangé J. J. 
Published: February 2025

In 2023, the International Renewable Energy Agency and the International Geothermal Association released a report underscoring the pivotal role of geothermal energy in the transition to a CO2-neutral energy system. In Europe, where over 50% of energy demand is driven by seasonal heating, geothermal systems are crucial for the heat transition. However, uncertainty in well productivity and high upfront costs for well construction present significant financial risks, slowing market adoption. Drilling of horizontal and multi-lateral wells is known for the potential of productivity enhancement by increasing connectivity with the reservoir. The productivity performance increase for geothermal reservoir cases has been demonstrated by modelling various lateral configurations (Peters et al., 2015). Due to the high drilling costs for horizontal wells and hence, for multi-lateral structures, utilization of this concept is often not a viable, economic option for a geothermal project. The challenge lies in making their construction cost-effective for geothermal operators.
A study for Dutch geothermal applications (Peters et al., 2015) demonstrated that the productivity improvement factor (PIF) scales linearly with the total lateral well length placed (Figure 1a) and highlighted that the PIF is dependent on the ratio of vertical (kv) to horizontal (kh) reservoir permeability and the reservoir thickness (Figure 1b). PIFs up to three can be realized with a structure of multiple 100m laterals in a tight sandstone reservoir.
Simpler and low-cost clean water jet-based system for lateral drilling are available on the market since many years. Commercial jet drilling systems usually aren't suitable for very competent rock types. They are working best for rocks with higher porosity and lower mechanical strength. The length of these laterals is limited to a maximum of about 100m, jetted wellbore diameters are about one inch. These clean water jet drilling tools have no directional control resulting in a more tortuous drill paths (e.g., Reinsch et al., 2018). The success rate with respect to drilled length and predicted productivity enhancement shows a higher level of variability. The applicability, potential, and limitations of these un-steered, clean water jet drilling systems, for realization of lateral structures, have been comprehensively investigated within the EU HORIZON 2020 project SURE (https://www.sure-h2020.eu/).