bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lactobacillus johnsonii Generates Cyclo(pro‐trp) and Promotes Intestinal Ca2+ Absorption to Alleviate CKD–SHPT
Authors: Zeng Xiong , Sun Lihua , Xie Huichao , Gong Shenhai , Lu Caibao , Xu Zhongwei , Guan Haidi , Han Ben , Wang Wei , Zhang Zhengmin , Zhou Jieying , Wang Shuai , Chen Yihui , Xiao Weidong 
Published: January 2025

AbstractPatients with chronic kidney disease (CKD) are at a high risk of developing secondary hyperparathyroidism (SHPT), which may cause organ dysfunction and increase patient mortality. The main clinical interventions for CKD–SHPT involve calcium supplements to boost absorption, but ineffective for some patients, and the reasons remain unclear. Here, CKD mice are divided into high and low groups based on intact parathyroid hormone (iPTH) levels. The high group exhibits significant changes in gut microbes, including a decrease in Lactobacillus, an increase in parathyroid hyperplasia, and a decrease in intestinal calcium. Fecal microbiota transplantation and L. johnsonii colonization indicate a link between gut microbes and CKD–SHPT. Clinically, higher L. johnsonii levels are correlated with milder hyperparathyroidism CKD–SHPT. The receiver operating characteristic (ROC) curve for L. johnsonii abundance and surgical risk is 0.81, with the calibration curve confirming predictive accuracy, and decision curve analysis revealing good clinical applicability. In vivo and in vitro experiments show that cyclo(pro‐trp) enhance calcium inflow and lower iPTH levels in intestinal epithelial cells via a calcium‐sensing receptor and transient receptor potential vanilloid 4 pathways. This study identified the crucial role of L. johnsonii in CKD–SHPT, unveiling a new mechanism for calcium imbalance and offering novel strategies for SHPT treatment and drug development.
The Negative Role of Proton Insertion on the Lifetime of Vanadium‐Based Aqueous Zinc Batteries
Authors: Zhu Chaoqiong , Zheng Limin , Ruan Hao , Xiao Meng , Ye Meng , Chen Ting , Wan Fang , Guo Xiaodong 
Published: January 2025

AbstractVanadium oxides are attracted cathodes for aqueous zinc batteries owing to their high capacity. However, the limited cyclability of vanadium‐based oxide cathodes, especially at low current densities, impedes their practical application. Here, it is revealed that proton insertion is responsible for the limited lifetime of vanadium oxides. Proton insertion promotes the dissolution of vanadium oxides, deteriorating electrochemical performance. Propylene carbonate (PC) is introduced into Zn(CF3SO3)2 electrolyte to regulate the coordination environment of water, forming PC‐coordinated Zn2+ solvation structure and [H2O‐CF3SO3−‐PC] complex. The optimized coordination environment of water weakens the adsorption energy between water molecules and vanadium oxides, inhibiting proton insertion. As a result, vanadium‐based oxides cathode without proton insertion can maintain the stability of crystal structure and avoid the dissolution of V. Taking CaV8O20·nH2O as cathode, Zn||CaV8O20·nH2O battery without proton insertion performs enhanced cycling performance. This work not only reveals the negative effect of proton insertion on the lifetime of vanadium‐based oxides cathode but also provides an effective strategy to modulate proton insertion.
Inversion of Circularly Polarized Luminescence in the Left‐Handed Chitosan‐Templated Co‐assemblies
Authors: An Yu , Shen Zhaocun , Zhang Fang , Yang Qiuya , Han Zihan , Wang Mingjie , Ma Hongze , Yu Linjie , Yuan Wei , Sui Kunyan 
Published: January 2025

AbstractCircularly polarized luminescence (CPL) materials are attractive due to their unique applications in fields such as 3D displays, information encryption, and chiroptical switches. Natural biomolecules‐based CPL materials are gaining plenty of attention due to their chiral diversity and sustainability. However, it is still challenging to construct CPL materials with opposite CPL signs from a single natural biomolecule due to its inherent chirality. Here, chiral assemblies with opposite CPL signs using chitosan oligosaccharide (COS) and achiral luminescent dyes are successfully prepared. It is found that COS can serve as a chiral template to induce the ordered assembly of the dyes along the polymer chain through electrostatic attraction interaction. It is demonstrated experimentally that the structural planarity of the dye molecules is crucial for the formation of chiral co‐assemblies. Interestingly, the left‐handed COS‐templated co‐assemblies can emit CPL with opposite handedness, which is controlled by the helicity degree of the co‐assemblies. This study not only deepens the understanding of the complex assembly of natural biomacromolecules but also provides new insights into the design and construction of CPL materials.
Evolutionary Pro‐To‐Thr Mutation in the Intrinsically Disordered Domain of ANP32 Family Members Modulates Their Target Binding Modes
Authors: Baños‐Jaime Blanca , Uceda‐Mayo Ana B. , Rivero‐Rodríguez Francisco , Casado‐Combreras Miguel Á. , Velázquez‐Cruz Alejandro , Velázquez‐Campoy Adrián , Corrales‐Guerrero Laura , De la Rosa Miguel A. , Díaz‐Moreno Irene 
Published: January 2025

AbstractGene duplication has allowed protein evolution toward novel functions and mechanisms. The differences between paralogous genes frequently rely on the sequence of disordered regions. For instance, in mammals, the chaperones ANP32A and ANP32B share a common evolutionary line and have some exchangeable functions based on their similar N‐terminal domains. Nevertheless, their C‐terminal low‐complexity‐acidic‐regions (LCARs) display substantial sequence differences, unveiling some degree of variability between them, in agreement with their different tissue‐specific expression patterns. These structural and computational results indicate that a substitution in the vicinity of the nuclear localization signal (NLS), of Pro in ANP32A for Thr in ANP32B, determines the overall compactness of the C‐terminal LCAR. The different structural properties of the disordered region affect the binding mode of ANP32 members to their targets. This type of divergent binding mode is exemplified with the extra‐mitochondrial cytochrome c (Cc), a well‐known ANP32B partner and which now determine also binds to ANP32A; and with the RNA binding protein HuR, whose export to the cytoplasm is mediated by ANP32 proteins under stress. Therefore, differential expression patterns of ANP32A or ANP32B may affect the regulation of Cc and HuR and can help to explain the distinct roles of these proteins in diseases.
Machine Learning‐Powered Optimization of a CHO Cell Cultivation Process
Authors: Richter Jannik , Wang Qimin , Lange Ferdinand , Thiel Phil , Yilmaz Nina , Solle Dörte , Zhuang Xiaoying , Beutel Sascha 
Published: January 2025

ABSTRACTChinese Hamster Ovary (CHO) cells are the most widely used cell lines to produce recombinant therapeutic proteins such as monoclonal antibodies (mAbs). However, the optimization of the CHO cell culture process is very complex and influenced by various factors. This study investigates the use of machine learning (ML) algorithms to optimize an established industrial CHO cell cultivation process. A ML algorithm in the form of an artificial neural network (ANN) was used and trained on datasets from historical and newly generated CHO cell cultivation runs. The algorithm was then used to find better cultivation conditions and improve cell productivity. The selected artificial intelligence (AI) tool was able to suggest optimized cultivation settings and new condition combinations, which promised both increased cell growth and increased mAb titers. After performing the validation experiments, it was shown that the ML algorithm was able to successfully optimize the cultivation process and significantly improve the antibody production. The best results showed an increase in final mAb titer up to 48%, demonstrating that the use of ML algorithms is a promising approach to optimize the productivity of bioprocesses like CHO cell cultivation processes clearly.
Internationalisation, Waste Management and Board Attributes
Authors: Uyar Ali , Al‐Shaer Habiba , Kuzey Cemil , Karaman Abdullah S. 
Published: January 2025

ABSTRACTWe investigate whether internationalisation is significantly associated with waste management. Secondly, by focusing on two critical board attributes, we investigate whether female and tenured directors help enable internationalised firms' better waste management. We find that more internationalised firms produce more waste; this result is robust to various waste proxies such as total waste, hazardous and nonhazardous waste and waste scaled by turnover. Although they tend to engage with less recycling, the result is insignificant. Furthermore, we find that both female and tenured directors significantly moderate between internationalisation and waste management; they help reduce waste in internationalised firms. However, they cannot significantly moderate between internationalisation and waste recycling, which seems a missing link in better waste management of internationalised firms. The results imply that multinationals pollute the environment by producing more waste and not engaging in waste recycling. Given the cross‐border scale of their manufacturing, sales and/or logistics operations, the findings are of critical importance for multinationals, their governance structure and stakeholders. We posit that international firms are more exposed to visibility and hence are under the scrutiny of stakeholders such as regulatory bodies, the press and environmentalists. Waste production and lack of waste recycling might trigger legitimacy concerns and incompatibility sanctions.
P‐min‐Stable Regression Models for Time Series With Extreme Values of Limited Range
Authors: Nascimento Leonardo Brandao Freitas , Lima Max Sousa , Duczmal Luiz H. 
Published: January 2025

ABSTRACTIn this paper, a P‐min‐stable regression model is proposed for a time series of extreme values observed in a limited interval. The model may be useful when the variable or indicator of interest is the minimum value of a series restricted to the unit interval and is related to other variables through a regression structure. The serial extremal dependence is induced through the marginalization of the Kumaraswamy distribution conditioned on a latent ‐stable process. The model is flexible to capture trends, seasonality, and non‐stationarity. Some properties of the model are presented, as well as the extremogram of the series. Procedures for estimation and inference are discussed and implemented via an Expectation‐Maximization algorithm. As an illustration, the model was used to analyze the minimum relative humidity observed in the Brazilian Amazon.
Role Identity, Loneliness, and Bereavement During the Pandemic in Japan: A Cross‐Sectional Study
Authors: Toyoshima Aya 
Published: January 2025

ABSTRACTBackground and AimsThe COVID‐19 pandemic significantly restricted social activities, prompting a re‐examination of community dynamics. In Japan, where families are central, the absence of a spouse increases susceptibility to loneliness and isolation. The loss of a spouse, especially during the pandemic, has heightened these issues among middle‐aged and older individuals. Limited quantitative studies on pandemic‐related bereavement in Japan underscore the need to understand the associated risks of loneliness and social isolation. This study aimed to examine the relationships between role identity as a member of the local community, loneliness, and social support among middle‐aged and older adults. This study also examined whether there were differences in the relationships between those who had experienced bereavement and those who had not.MethodsAn Internet survey was conducted with Japanese adults (aged 50–74 years), including those who experienced bereavement during the pandemic. The final sample included 322 participants (154 in the bereavement group and 168 in the cohabiting group). The variables, measured using psychological scales, included role identity as a local resident, social capital (trust, reciprocity, and membership), loneliness, and social support (family and friendship).ResultsThe higher trust and role identity were related to lower loneliness. Membership in the cohabiting group was related to lower level of loneliness and higher level of social supports. However, membership in the bereavement group was not related to either. Furthermore, trust and reciprocity were related to loneliness and social support.ConclusionThese results indicated that the role identity and perception of social capital related to preventing isolation and loneliness for bereaved people.
A Perspective on Lung Cancer and Lung Microbiome: Insight on Immunity
Authors: Emadi Reza , Saki Sasan , Yousefi Parastoo , Tabibzadeh Alireza 
Published: January 2025

ABSTRACTBackgroundAlthough the carcinogenic potential of microbes has long been recognized, their significance may have been underestimated. Currently, the connection between microbiota and cancer is under extensive research. The lung microbiota may serve as a proxy for the state of lung health based on its crucial role in preserving lung hemostasis.ObjectivesThis review tried to outline the state of our understanding of the contribution of lung microbiome and lung cancer.MethodsA literature search was performed using PubMed, Google Scholar, and Scopus databases for recent research focusing on the development and possible pathogenesis of lung microbiome and lung cancer.ResultsEarly research on lung cancer indicated that dysbiosis significantly impacted the development and spread of the tumor. As a result of these findings, the study of the lung microbiota as a possible therapeutic target and diagnostic marker has accelerated. Early‐stage disease diagnostic biomarkers could be represented as microbiota profiles. Additionally, the microbiome is involved in anticancer therapy. There are limited studies on lung microbiota, and most microbiome studies commonly concentrate on the gut microbiota. A proper understanding of lung microbiota can have several potential therapeutic approaches. Therefore, more studies in this field may initiate remarkable advancements in microbiome‐dependent treatment.ConclusionConvincing data from studies on both humans and animals indicates that the microbiota might play a role in cancer initiation, influenced by internal and environmental factors of the host. Notably, the lung harbors its microbiome, as do lung cancers. In general view, it seems microbiome diversity in lung cancer patients is reduced. Meanwhile, some genera were increased in lung cancer patients in comparison with a noncancerous population (such as Streptococcus genus), and some of them were decreased (Granulicatella adiacens, G. adiacens). Furthermore, research on the microbiome‐carcinogenesis relationship is still in its infancy, and much remains to be fully understood.
Human Versus Robot: Comparing Service Agents in Hospitality Settings—Insights From a Field Study
Authors: Schmidt Alexander Lennart , Koerten Klaas , Tuomi Aarni , El‐Manstrly Dahlia , Wiegerink Karoline 
Published: January 2025

ABSTRACTThe hospitality industry is facing personnel challenges, including personnel shortages and high staff turnover, asking practitioners to explore service robots to enhance competitiveness. While prior academic studies have primarily relied on hypothetical or conceptual research, there remains a pressing need for real‐life field studies to assess the practical impacts of service robots in hospitality. This study examines the comparative effects of human and robotic service agents at the touchpoint of information provision in a real‐world hotel setting. Using a field experiment with 200 participants, where both service agents (human and robot) were simultaneously available, we assess service agent's impact on guests' experience of hospitality, satisfaction, and revisiting intentions. The findings reveal indifferent effects between human and robotic agents, challenging assumptions that robots negatively impact guest experiences. Contrary to debates suggesting that human agents are superior in hospitality roles, our results indicate that service robots can effectively complement human staff, reducing demand for frontline personnel and lowering operational costs without diminishing guest satisfaction. This study highlights the potential of integrating robotic agents into the hospitality frontline, particularly for routine tasks like information provision. We acknowledge limitations, including the focus on a single touchpoint, and call for broader research across diverse guest interactions and touchpoints. Future studies should also explore the underlying factors influencing guests' choice of service agents. These findings offer practical implications for tackling labor shortages while maintaining service quality, providing actionable insights for the hospitality industry in the context of digital transformation.
Rapid screening and identification of novel dipeptidyl peptidase IV inhibitory peptides from buffalo milk
Authors: Zheng Qianqian , Luo Xin , Pan Daodong , Wang Yanli , Suo Shikun , Dang Yali , Gao Xinchang 
Published: January 2025

AbstractBACKGROUNDPeptidomics combined with molecular docking is an effective alternative method for rapid screening of novel bioactive peptides in food. Buffalo milk as a potential source of dipeptidyl peptidase‐IV (DPP‐IV) inhibitory peptides has been less studied. Peptidomics and molecular docking methods were employed to rapidly screen new DPP‐IV inhibitory peptides from buffalo milk. The screened DPP‐IV inhibitory peptides were further verified using an in vitro inhibition assay and a Caco‐2 cell assay.RESULTSThe DPP‐IV inhibition rate of buffalo milk was increased from 73.40 ± 6.01% to 97.23 ± 3.18% in an in vitro digestion assay, suggesting that buffalo milk could be a promising source of DPP‐IV inhibitory peptides. Subsequently, two novel peptides (GPFPIIV and FPQYL) with potential DPP‐IV inhibitory activity were screened using peptidomics, molecular docking and an in vitro inhibitory assay. The IC50 values for GPFPIIV and FPQYL were 0.2998 ± 0.03 and 0.1407 ± 0.01 mg mL−1, respectively. During simulated gastrointestinal digestion in vitro, FPQYL had an excellent digestive stability of 92.13 ± 1.03%, whereas that of GPFPIIV was 59.52 ± 2.56%. In addition, GPFPIIV and FPQYL (1.00 mg mL−1) showed significant DPP‐IV inhibitory effects in a Caco‐2 cell assay, with the inhibition rate increasing to 32% and 36%, respectively.CONCLUSIONIn summary, two new DPP‐IV inhibitory peptides were screened from buffalo milk through a combination of peptidomics and molecular docking, both of which exhibited significant DPP‐IV inhibitory activities. The identified peptides, GPFPIIV and FPQYL, have promising applications in diabetes management. © 2025 Society of Chemical Industry.
#Fittok: How Fitfluencers' Videos on Tiktok Impact Adolescents' Body Satisfaction, Workout Intention, and Behavior
Authors: de Brabandere Marloes , Hudders Liselot , Vanwesenbeeck Ini 
Published: January 2025

ABSTRACTFitfluencers often flaunt their ideal bodies on TikTok. Additionally, they frequently demonstrate workouts and give authentic insights into their lives. Through three experiments, we examine how these videos impact adolescents' (14–18 years) body satisfaction and workout intention and behavior. Study 1 (N = 221) shows no significant difference between fitfluencers' posing and workout TikTok videos on body satisfaction and workout intention. The Solomon four‐group design of Study 2 (N = 176) shows that fitfluencers' posing TikTok videos (vs. lifehack videos) induce more appearance comparison and more contrastive comparison feelings, which lead to less body satisfaction. Effects on workout intention were not significant. Study 3 (N = 154) similarly shows that fitfluencers' videos (posing and authenticity vs. lifehack), induce more appearance comparison and more contrastive comparison feelings, which lead to less body satisfaction, as in Study 2. They also induce more assimilative comparison feelings, which lead to a greater workout intention. Exercise behavior did not differ across all three video types. As in Study 1, no significant differences between fitfluencers' videos (posing and authenticity) were found. This study contributes to social comparison theory and fitfluencer literature by showing that assimilative and contrastive comparison feelings drive different dependent variables.
Thermal and fatigue failure properties of graphene oxide/acrylonitrile butadiene rubber composites: New perspectives on the role of interface and interphase
Authors: Nargesi Azam Faezeh , Alimardani Mohammad , Shokoohi Shirin 
Published: January 2025

AbstractDamages originating from self‐generated heat in rubbers with low thermal conductivity properties constitute a major share of failure in many rubber applications. There are still contradictory results concerning the direction through which the nanoscale phenomena including the interface strength, the interphase as a layer with slowed‐down dynamics, and the filler dispersion contribute to the thermal conductivity of nanocomposites. Thermal failure properties depend on the net effect of the interfacial phenomena on the thermal accumulation and conductivity. Two grades of NBR (Acrylonitrile–Butadiene Rubber) having various levels of Acrylonitrile (ACN) content were reinforced with Graphene oxide (GO) at low (0.5) and high (3) parts per hundred rubber (phr) to create the diverse range of polymer–filler and filler–filler interactions. Thermodynamically‐based indicators relying on the surface energy of the components were employed in conjunction with the experimental means (scanning electron microscopy) to quantify the degree of interface strength, the interphase, and the dispersion quality. Measuring the thermal conductivity properties of the prepared samples, it was found that NBR40 containing 3 phr of GO, while presenting the highest volume of interphase and interface strength, shows the maximum thermal accumulation. It was found that interphase had no negative effect on thermal conductivity, and thus NBR40 also exhibited maximum thermal conductivity. The combinatory effect of thermal accumulation and conductivity on failure was further monitored by performing a high‐frequency fatigue crack growth experiment.Highlights
Evaluating role of interfacial phenomena on the thermal and fatigue failure
Quantifying the degree of interface strength, the interphase and the dispersion
Formation of interphase had no negative impact on the thermal conductivity
Stiffness of interphase layer was found to be the cause of better conductivity
Filler volume effects had a priority role on conductivity than surface effects