bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacterial dynamics and functions driven by compound microbial agents to simultaneously promote organic degradation and mitigate odor emissions during in-situ kitchen organic waste
Authors: Zou Shuping , Luo Yu , Ding Yiran , Liu Jinlong , Xue Yaping , Zheng Yuguo 
Published: December 2023

Abstract
The treatment of organic waste (OW) still faces the problems of large amounts of odor gas emissions and incomplete organic degradation. Therefore, to investigate the feasibility of simultaneously promoting organic matter degradation and mitigating odor emissions during in‑situ rapid biological reduction (IRBR) of organic waste, a study of the addition of compound microbial agents containing odor-reducing bacteria (ORB) and organic-degrading bacteria (ODB) was conducted. Results revealed that the co-addition biochar and compound microbial agents greatly achieved a higher organic degradation rate of 20.09% and decreased cumulative NH3 and H2S emissions of 45.14% and 27.25%, respectively. The microbial community analysis indicated that adding compound microbial agents reshaped microbial community composition throughout the process. With the addition of compound microbial agents, the inoculated Kocuria rosea and Acetobacter pasteurianus belonging to Proteobacteria gradually became dominant genera, and the abundance of Firmicutes was reduced which was positively correlated with NH3. These results revealed the potential microbial mechanism by which microbial communities influence odor release and organic degradation during IRBR process and indicated that inoculating compound microbial agent is a suitable strategy given the comprehensive emission reduction and biodegrading performance considerations.
Design and Validation of a Wearable Dynamometry System for Knee Extension-Flexion Torque Measurement
Authors: Park Sungwoo , Myong Youho , Cho Minwoo , Cho Seung Yeon , Lee Woo Hyung , Oh Byung-Mo , Kim Sungwan 
Published: December 2023

Abstract

Muscle strength assessments are vital in rehabilitation, orthopedics, and sports medicine. The significance of muscle strength lies in its ability to predict mortality and enhance quality of life. Muscle mass and strength can be effectively assessed using quadriceps and hamstrings. Conventional methods, including manual muscle tests and handheld dynamometers, are widely used but lack quantification and are rater-dependent. Isokinetic dynamometry (IKD), such as Biodex, is trustworthy but costly and limited in access; therefore, this study suggests a novel wearable dynamometry system (WDS) with knee-joint torque sensors and evaluated its efficacy in knee extension and flexion in 39 healthy adults. Comparing maximal isometric torque measurements, WDS and IKD showed high correlation and reliability for extension (Pearson’s r: 0.900; intraclass correlation coefficient [ICC]: 0.893; standard error of measurement [SEM]: 9.85%; minimal detectable change [MDC]: 27.31%) and flexion (Pearson’s r: 0.870; ICC: 0.857; SEM: 11.93%; MDC: 33.07%). WDS demonstrated excellent inter-rater (Pearson’s r: 0.990; ICC: 0.993; SEM: 4.05%) and inter-session (Pearson’s r: 0.970; ICC: 0.984; SEM: 6.15%) reliability during extension/flexion. User feedback from 35 participants, including healthcare professionals, underscores WDS's positive user experience and clinical potential. The proposed WDS is a suitable alternative to IKD, providing high accuracy, reliability, and potentially greater accessibility.
Genome features and metabolic properties of Ca. Methylomicrobium oryzae, the first Methylomicrobium member isolated from rice fields
Authors: Rahalkar Monali C. , Khatri Kumal , Pandit Pranitha , Mohite Jyoti A. 
Published: December 2023

Abstract
Methanotrophs represent a group of bacteria that oxidize methane and are therefore environmentally very important. The cultivation of methanotrophs poses a challenge due to their specific cultivation conditions. Rice fields are important habitats where methanotrophs play a vital role in methane mitigation. Very few laboratories in the world culture methanotrophs. We recently cultured Methylomicrobium strain RS1 and reported it as a member of putative novel species (Ca. Methylomicrobium oryzae) using draft genome-based ANI and DDH parameters [1]. In the present work, we present the polyphasic characterization of this novel species which is the first species of Methylomicrobium being reported from rice fields. Ca. Methylomicrobium oryzae is the first cultured methanotroph to be described from rice field habitats and could be an important player in these ecosystems and awaits further studies. Methylomicrobium sequences have been reported from rice fields but there were no reports of cultivation of members from this group, and ours was the first report. The culture was difficult and slow in growth and could not be cryopreserved. Therefore, it cannot be deposited in two international culture collections and has to be maintained as a live culture in our laboratory. In the present study, we describe the biochemical characteristics, metabolic properties, and salient genome features of Ca. Methylomicrobium oryzae strain RS1.
Lightweight insulating oil-well cement filled with hollow glass microspheres and numerical simulation of its unsteady heat transfer process
Authors: Wang Hui , Ma Chong , Yuan Yihui , Chen Yanglei , Liu Tao , Wang Ning 
Published: December 2023

Abstract

During subsea natural gas extraction, the free water and gas molecules present in the reservoir and the low-temperature and high-pressure conditions of the subsea environment cause the formation of hydrates; the blockage of the wellbore due to these hydrates is a critical issue that affects flow safety. Located between the wellbore and casing, well cement plays an important role in strengthening the wellbore and sealing off the oil, gas, and water layers. A cement that exhibits optimal mechanical strength and enhanced thermal insulation properties can contribute to preventing hydrate formation. However, research on such materials is rare. In this study, lightweight and thermally insulated (LWTI) composites with the desired mechanical strength for deep-sea natural gas development were prepared using oil-well cement (OWC) as the matrix and hollow glass microspheres (HGM) as the filler. A two-phase mathematical model of the HGM/OWC LWTI composites was developed using the COMSOL Multiphysics software and solved using the finite element method. A transient heat transfer analysis of the HGM/OWC LWTI composites was performed. The effective thermal conductivities (keff) of the HGM/OWC LWTI composites were measured and the values agreed well with the simulation results. The keff of the composites was approximately 0.371 W/(m·℃) when the HGM (D51.8) content was 40 vol.%. Compared to the traditional OWC (thermal conductivity ~ 0.889 W/(m·℃)), the thermal insulation performance of the HGM/OWC LWTI composites was significantly improved. In addition, the density, mechanical properties, and water absorption of the HGM/OWC LWTI composites were investigated. The densities of the HGM/OWC LWTI composites were found to be low, ranging from 1.31 to 1.94 g/cm3. The HGM/OWC LWTI composites exhibited good compressive strength and low permeability. Thus, HGM/OWC LWTI has promising applications in the thermal insulation of cemented wellbores for deep-sea natural gas development.
The kinship coefficient but not male coloration predicts early offspring growth in lake char
Authors: Wedekind Claus , Garaud Laura , Nusbaumer David , Cunha Lucas Marques da , de Guttry Christian , Ançay Laurie , Atherton Audrey , Lasne Emlien 
Published: December 2023

Abstract
The ‘good genes’ hypotheses of sexual selection predict that females prefer males with strong ornaments because they are in good health and vigor and can afford the costs of the ornaments. A key assumption of these models is that male health and vigor are useful predictors of genetic quality and hence offspring performance. We tested this prediction in wild lake char (Salvelinus umbla) whose breeding coloration is known to reveal aspects of male health. We first reanalyzed results from sperm competition trials in which embryos of known parenthood had been raised singly in either a stress- or non-stress environment. Male coloration did not correlate with any measures of offspring performance. However, offspring growth was reduced with increased kinship coefficients between the parents. To test the robustness of these first observations, we collected a new sample of wild males and females, used their gametes in a full-factorial in vitro breeding experiment, and singly raised about 3,000 embryos in either a stress- or non-stress environment (stress induced by microbes). Again, male coloration did not predict offspring performance while offspring growth was reduced with increasing kinship between the parents. We conclude that, in lake char, the genetic benefits of mate choice would be strongest if females could recognize and avoid genetically related males, while male breeding colors may be more relevant in intra-sexual selection.
A Systematic pan-cancer analysis of ATIC as a prognostic biomarker in human tumors
Authors: Li Hui , Zhang Wen-Tao , Zhou Xiao-jie , Wu Wei-ming , Yao Fu-wen , Zhong Hao-jie , Zhan Nai-yang , Chen Jian-Wei , Zhan Yong-Qiang 
Published: December 2023

Abstract
Background
5-Aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) has been identified as a potential biomarker for cancer. However, the biological functions and implications of ATIC in pan-cancer context remain largely unexplored.
methods
We utilized The Cancer Genome Atlas (TCGA) database to acquire RNA-seq and clinical follow-up data for 33 different tumor types. This data was analyzed to investigate the expression patterns and biological functions of ATIC across pan-cancer, encompassing diverse molecular and immune subtypes. The prognostic and diagnostic significance of ATIC in a pan-cancer context was evaluated using Kaplan-Meier and Receiver Operating Characteristic (ROC) curves. Finally, our study specifically focused on Liver Hepatocellular Carcinoma (LIHC) to elucidate the potential mechanisms underlying ATIC's role in LIHC.
results
ATIC expression was found to be up-regulated in a broad spectrum of tumor tissues, exhibiting significant variability across different molecular and immune subtypes. Furthermore, ATIC demonstrated high accuracy in cancer prediction and was notably correlated with the prognosis of various cancers. Specifically, in Liver Hepatocellular Carcinoma (LIHC), ATIC's expression was significantly linked to clinicopathologic features, a range of cancer-related pathways, the extent of immune cell infiltration, and m6A modifications.
conclusion
ATIC could potentially serve as a molecular biomarker for both the diagnosis and prognosis of pan-cancer. Additionally, it may play a significant role in the progression and metastasis of LIHC through various mechanisms.
Effect of chronic cold stress on gut microbial diversity, intestinal inflammation and pyroptosis in mice
Authors: Lv Hongming , Xia Shijie , He Yuxi , Qiao Chunyu , Liu Jiahe , Guo Jingru , Li Shize 
Published: December 2023

Abstract
Hypothermia is an essential environmental factor in gastrointestinal diseases, but the main molecular mechanisms of pathogenesis remain unclear. The current study sought to better understand how chronic cold stress affects gut damage and its underlying mechanisms. In this work, to establish chronic cold stress (CS)-induced intestinal injury model, mice were subjected to prolonged cold exposure (4°C) for 3 h per day for 3 weeks. Our results indicated that CS led to gut injury via inducing changes of heat shock proteins 70 (HSP70) and apoptosis-related (caspases-3, Bax and Bcl-2) proteins; enhancing expression of intestinal tight-related (ZO-1 and occludin) proteins; promoting releases of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), high mobility group box 1 (HMGB1), interleukin1β (IL-1β), IL-18 and IL-6 inflammatory mediators in the ileum; and altering gut microbial diversity. Furthermore, persistent cold exposure resulted in the cleavage of pyroptosis-related Gasdermin D (GSDMD) protein by regulating the NLRP3/ASC/caspase-1 and caspase-11 pathway, and activation of toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)-mediated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, which are strongly associated with changes in gut microbiota diversity. Taken together, these investigations provide new insights into the increased risk of intestinal disorders at extremely low temperatures and establish a theoretical foundation for the advancement of novel pharmaceutical interventions targeting cold-related ailments.
PIH1D1 as a potential biomarkers candidate for diagnostic approach in Breast Cancer
Authors: Singh Dhiraj Kumar , jit Bimal Prasad , Verma Amit Kumar , Pradhan Biswajita , Mir Riyaz Ahmad 
Published: December 2023

Abstract
The R2TP complex is a co-chaperone conserved from Yeast to mammals and comprises of RUVBL1, RUVBL2, PIH1D1 and RPAP3 in Human is known to be a specialized Co-chaperone of Hsp-90 protein. A number of multi-subunit complexes, such as RNA polymerase II, tiny nucleolar ribonucleoproteins, and complexes containing phosphatidylinositol-3-kinase-like kinases, are assembled and matured by this protein complex. The expression level of PIH1D1 were analyzed across cancers using TIMER 2 and compared normal and breast cancer tissues using TNM plot. Survival Plot were done by GEPIA. TCGA data analyzed for the evaluation of the expression of PIH1D1 in breast cancer and both Histological and Clusters and Interaction analysis with the help of Human Protein atlas Database. The results indicate that PIH1D1 is highly expressed in breast cancer than normal tissues. So, PIH1D1 can be used for early detection as well as indicators of prognosis of Breast. Moreover, PIH1D1 may become the target of immunotherapy for Breast cancer in the future. Our current study is limited, though, because the data we examined came exclusively from bioinformatics source. Through both in vivo and in vitro investigations, we will investigate the possible mechanism of PIH1D1 in breast cancer cell lines and clinical tissues.
Cattle intensification based on silvopastoral systems with Leucaena leucocephala (Lam.) de Wit as a strategy to increase cattle production and mitigate greenhouse gas emissions in the Caribbean region of Colombia
Authors: Rivera Julián E. , Villegas Gonzalo O. , Serna Laura P. , Chará Julián 
Published: December 2023

Abstract

Different cattle production interventions have been proposed to reduce greenhouse gas (GHG) emissions and increase animal productivity, but few studies have identified their real potential under grazing conditions and at the whole system level. The aim of this study was to determine the effect of silvopastoral systems (SPS) with Leucaena leucocephala on total GHG emissions, emission intensity, GHG balance and cattle productivity in a series of production scenarios in northern Colombia. A life cycle analysis (LCA) approach was used to evaluate one baseline system (BL) based on pastures and four SPS intervention scenarios. For this analysis, the emissions from enteric fermentation and manure were determined in situ. Methane emissions from enteric fermentation were 9.7% lower in the SPS with leucaena than in BL systems (p = 0.0369). Emission factors for manure were higher in the BL systems (p < 0.05) as were emission intensities to produce one kg of fat and protein corrected milk (FPCM) and one kg of live weight gain (LWG). For animal production and GHG balance, it was found that the inclusion of leucaena increased animal production up to four times compared to the BL system and mitigate GHG emissions per hectare up to 86% due to carbon sequestration. In conclusion, leucaena SPS are an alternative to mitigate climate change under grazing conditions, as they reduce GHG emissions and increase animal productivity.
Breast volume fluctuations are associated with oestradiol and progesterone changes across the menstrual cycle
Authors: Rix Jacqueline , Mills Chris , Ross Emma , Allen Sian , Lai Adrian , Wakefield-Scurr Joanna 
Published: December 2023

Abstract

Background:Breast volume changes across the menstrual cycle, but the relationship of this to oestradiol, progesterone and awareness of this change has yet to be characterised. Across the menstrual cycle, this study investigated relationships between breast volume, oestradiol, progesterone, and perceptions of volume change, with associated behaviour change.
Methods:Fifteen women undertook saliva hormone testing bidaily throughout one menstrual cycle. Women attended six laboratory appointments across their cycle (day 2, ovulation-2, ovulation, ovulation+7, menses-3, day 2 (month two)) for 3D surface scanning (breast volume), perception of volume and behaviour change was recorded.
Results:Breast volume changed by +7.3%, but up to -41.7% in one woman. Volume asymmetry increased around ovulation (5.1%). Breast volume change strongly correlated with oestradiol and progesterone, but was delayed by three appointments (left r=0.85; right r=0.95) and one appointment (left r=0.84; right r=0.84), respectively. For women whose volume decreased in follicular and increased in luteal phases (typical pattern; n=11) oestradiol decreased by 13.3%, compared to those who did not (n=4) (14.2%). Breast volume was not associated with perceived breast volume change.
Conclusion:
Average breast volume increases of ~one-third bra cup might not be meaningful; however, inter-participant variability was large, with up to ~1 bra cup change. As women did not accurately perceive their volume changes, measuring individuals’ cyclical breast volume would be useful for bra fitting. Volume peaked ~13 days after oestradiol and ~four days after progesterone peak. However, large variability makes it difficult to predict this; studies should take multiple samples across the cycle.
What is more important for structuring Dragonfly assemblages in Subtropical Grasslands lentic water bodies in the Pampa biome: space or vegetation?
Authors: Vareira Luísa , Ortega Jean Carlo Gonçalves , Juen Leandro , Sendoya Sebastian Felipe , Iserhard Cristiano Agra 
Published: December 2023

Abstract
For more than six decades, ecologists have been investigating how biological communities are structured and the implications for conservation. The Niche and Island Biogeography theories stand out as the basis for predictions related to diversity-environmental heterogeneity and diversity-area relationships, respectively. Although positive relationships are expected, the relative roles of environmental heterogeneity (EH) and area in structuring lentic water body assemblages are unclear for dragonflies. We sought to investigate the effects of area and EH, expecting that there would be a positive influence on dragonfly species richness and abundance. We sampled dragonfly adults for three months in 12 water bodies in the Pampa biome of Rio Grande do Sul, Brazil. We quantified water body area and vegetation, as an EH measure to evaluate how these predictors explained dragonfly species richness and abundance. We found that water body area, but not EH, was relevant to dragonfly diversity. This indicates that dragonflies may have perceived the vegetation as homogeneous and thus moved across the landscape without perceiving barriers that would hinder new habitat colonization. Therefore, the structure of water body dragonfly assemblages in Pampas grasslands was more influenced by water body area than environmental heterogeneity. Implication for insect conservation We highlight the relevance of tropical lentic water bodies in harboring species not registered yet, an important knowledge shortfall for biodiversity. We also found a high dragonfly diversity in large water bodies, which means that in managed landscapes the conservation of large areas is required to sustain this high diversity.
Understanding Turkish Students’ STEM Career Aspirations, STEM Hopes and Goals, Parental Perception, and Cultural Capital: A Path Analysis
Authors: KÜÇÜKAYDIN MENŞURE ALKIŞ , ULUM HAKAN 
Published: December 2023

Abstract

Developing students' career goals in the fields of science, technology, engineering, and mathematics (STEM) is a high priority in Turkey and around the world. Many educational investments are being made in the country, and this is shown in the development goals. It is important to understand the variables that may be related to these goals in order to best support students on their career path. This study examined the relationship between STEM career aspirations, hopes and goals in STEM, cultural capital, and perceived parental expectations in a Turkish sample (n = 633). The findings showed that there were positive relationships between STEM cultural capital, perceived parental support, and STEM-related hopes and goals. In addition, parental perception influenced STEM career aspirations both directly and indirectly. In the research model, the greatest effect was found to be between parental support and STEM hopes and goals. These discoveries provide insight for educational policymakers, parents, and teachers who are helping students develop STEM career aspirations.
Irrigation Management Strategies and Monitoring of Water Status through Infrared Thermography in Melon Production
Authors: Costa James do Nascimento , Gomes Francisco Fábio Lima , Aragão Márcio Facundo , Neto Luis Gonzaga Pinheiro , Azevedo Benito Moreira , Lima Joilson Silva , Costa Francisco Walisson do Nascimento , Frazão Diogo Sales 
Published: December 2023

Abstract

This study aimed to evaluate, through infrared thermography, the water status of melon crop during the fruiting phase and the productivity relationships resulting from fixed deficit irrigation (FDI) and regulated deficit irrigation (RDI) in two cultivation cycles. Nine irrigation treatments based on ETc (%) were applied, comprising four FDI treatments (T1- 125%; T2-100%; T3-75%; T4-50% throughout the cycle) and five RDI treatments (T5, T6, T7, T8, and T9) with replacements varying by 25% of ETc at stages I, II, III, and IV of the melon crop. Data on plant temperature (Tc) and air temperature (Ta) at 8:00, 12:00, and 16:00 h, leaf water potential (ΨF), gas exchange (gs and E), and soil moisture (SMO) were obtained at 45 days after transplanting in cycle I and at 42 days after transplanting in cycle II. Using the Tc and Ta data, thermographic indices ∆Tcanopy−air, CWSI, and IG were calculated. After harvesting, productivity and water use efficiency (WUE) were determined. Gas exchange did not strongly correlate with water replacement for FDI treatments in both conducted cycles. The ∆Tcanopy−air, CWSI, and IG indices significantly correlated with ΨF and SMO at the monitored times; however, 12:00 h was found to be the most suitable time for thermographic monitoring. Productivity and WUE were higher in the RDI treatments, with the T6 treatment (50% in phase I, 100% in phase II, and 75% in phases III and IV) standing out. Correlations between WUE and thermographic indices showed better results for RDI in cycle I, with both indices at 8 h and 12 h. In cycle II, better correlations were presented by FDI at both monitored.