bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exogenous Amyloid Sequences: Their Role in Amyloid-Beta Heterotypic Aggregation
Authors: Curto Jofre Seira , Hernandez Sandra Villegas , Fernandez Maria Rosario , Sanchez de Groot Natalia 
Published: January 2025

AbstractProtein aggregation is a complex process influenced by environmental conditions and interactions between multiple molecules, including those of exogenous origin. Althoughin vitrosimulations of aggregation are crucial for advancing research, few studies explore cross-seeding as a repeating event, despite the potential for such events when proteins circulate through the body. Here, we investigated the impact of exogenous amyloid sequences derived from the gut microbiota on the heterotypic aggregation of Aβ peptides. We utilized ten 21-amino acid peptides derived from bacterial genomes, previously shown to interfere with Aβ40 aggregation and induce memory loss inCaenorhabditis elegans. Through consecutive cross-seeding assays with Aβ40 and Aβ42, we analyzed the effects of these peptides on aggregation kinetics and seed propagation. Our findings indicate that exogenous molecules can influence Aβ’s aggregation process, altering the fibrils’ properties. Based on this, we introduce the “Interaction History” concept, where prior interactions shape the aggregation and propagation of Aβ peptides. This work supports the idea that environmental factors, such as microbial amyloids, can contribute to the heterogeneity and progression of amyloid-related diseases. Our results highlight the need for therapeutic strategies targeting diverse amyloid configurations and underscore the importance of considering exogenous sequences as additional triggers in AD pathology.
A 96well ultrafiltration approach for the high-throughput proteome analysis of extracellular vesicles isolated from conditioned medium
Authors: Pauwels Jarne , Van de Steene Tessa , De Muyer Freya , De Pauw Danaë , Baeke Femke , Eyckerman Sven , Gevaert Kris 
Published: January 2025

1.AbstractExtracellular vesicles (EVs), nanoscale vesicles that are secreted by cells, are critical mediators of intercellular communication and play a crucial role in diverse pathologies such as cancer development. Therefore, EVs are regarded as having high potential in the clinic, both for diagnostic and therapeutic applications. Unfortunately, EVs reside in complex biofluids and their consistent isolation at sufficient purity for mass spectrometry-based proteomics has proven to be challenging, especially when increased high-throughput is required. Here, we describe the incorporation of our previously reported filter-aided EV enrichment (FAEVEr) strategy for the isolation of EVs from conditioned medium, from harvest to proteomic analysis completely to a streamlined 96well format. We compared our approach with ultracentrifugation, the most widely used method for EV enrichment, in terms of protein identifications, consistency, reproducibility and overall performance, including the invested time, resources and required expertise. In addition, our results show that including relative high percentages of TWEEN-20, a mild detergent, markedly improves the final purity of the EV proteome by removing the bulk of non-EV proteins (e.g. serum proteins) and significantly increases the number of identified transmembrane proteins. Moreover, our FAEVEr 96well strategy improves the overall reproducibility with a consistent number of protein identifications and decreased number of missing values across replicates. This promotes the validity and comparability between results, which is essential in both a clinical and research setting, where consistency is paramount.3.Graphical abstract
Path of differentiation defines human macrophage identity
Authors: Frenis Katie , Badalamenti Brianna , Mamigonian Ohanez , Weng Chen , Wang Dahai , Fierstein Sara , Côté Parker , Khong Hesper , Li Hojun , Lummertz da Rocha Edroaldo , Sankaran Vijay G. , Rowe R. Grant 
Published: January 2025

SUMMARYMacrophages play central roles in immunity, wound healing, and homeostasis - a functional diversity that is underpinned by varying developmental origins. The impact of ontogeny on properties of human macrophages is inadequately understood. We demonstrate that definitive human fetal liver (HFL) hematopoietic stem cells (HSCs) possess two divergent paths of macrophage specification that lead to distinct identities. The monocyte-dependent pathway exists in both prenatal and postnatal hematopoiesis and generates macrophages with adult-like responses properties. We now uncover a fetal-specific pathway of expedited differentiation that generates tissue resident-like macrophages (TRMs) that retain HSC-like self-renewal programs governed by the aryl hydrocarbon receptor (AHR). We show that AHR antagonism promotes TRM expansion and mitigates inflammation in models of atopic dermatitis (AD). Overall, we directly connect path of differentiation with functional properties of macrophages and identify an approach to promote selective expansion of TRMs with direct relevance to inflammation and diseases of macrophage dysfunction.
BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A-HDAC1
Authors: Monziani Alan , Ben-Tov Perry Rotem , Hezroni Hadas , Ulitsky Igor 
Published: January 2025

AbstractChromatin modifications play a key role in regulating gene expression during development and adult physiology. Histone acetylation, particularly H3K27ac, is associated with increased activity of gene regulatory elements such as enhancers and promoters. However, the regulation of the machinery that write, read, and erase this modification remains poorly understood. In particular, the SIN3A-HDAC1 complex possesses histone deacetylase activity, yet it commonly resides at active regulatory regions. Here, we study BAHCC1, a large chromatin-associated protein essential for viability, and recently reported to play a largely repressive role. We show that in neuronal lineage cells, BAHCC1 is mainly associated with regulatory elements marked with H3K27ac. BAHCC1 interacts and co-occupies shared genomic regions with the SIN3A scaffold protein, and its perturbations lead to altered acetylation and expression of proximal genes in a neuronal cell line and primary cortical neurons. The regulated genes are enriched for those functioning in neurogenesis and cell migration, and primary cortical neurons with reducedBahcc1expression display impaired neurite outgrowth. We thus propose a model in which BAHCC1 antagonizes SIN3A histone deacetylation and positively regulates the expression of genes that are important for growth and migration-related processes in the neuronal lineage.
Physiological analysis of the mechanism of Ci transcription factor activation through multiple Fused phosphorylation sites in Hedgehog signal transduction
Authors: Kim Hoyon , Little Jamie , Li Jason , Patel Bryna , Kalderon Daniel 
Published: January 2025

AbstractHedgehog (Hh) proteins elicit dose-dependent transcriptional responses by binding Patched receptors to activate transmembrane Smoothened (Smo) proteins. Activated Smo inhibits Ci/Gli transcription factor phosphorylation by Protein Kinase A (PKA) and consequent proteolytic processing to repressor forms; it also promotes nuclear transport and activity of full-length Ci/Gli proteins to induce Hh target genes. Smo-activated Fused (Fu) kinase drives Ci activation in Drosophila, while Suppressor of Fused (Su(fu)) counters full-length Ci/Gli activity and stabilizes full-length Ci/Gli by direct binding to at least three surfaces. Here, we used CRISPR-generated designercialleles to investigate alterations to Fu phosphorylation sites and to regions around Ci-Su(fu) interfaces under physiological conditions in Drosophila imaginal wing discs. Surprisingly, we identified alterations that activate Ci without significant loss of stabilization by Su(fu) and contributions of multiple Fu target sites to Ci activation in the absence of Su(fu), suggesting that the affected sites mediate Ci activation by regulating Ci-Ci, rather than Ci-Su(fu) interactions. We propose that those interactions maintain full-length Ci in a closed conformation that also facilitates, and is stabilized by, cooperative Ci-Su(fu) binding. Access to binding partners necessary for Ci activation is promoted through phosphorylation of at least four Fu sites on Ci, likely by directly disrupting Ci-Ci contacts and one Ci-Su(fu) interface without substantial Ci-Su(fu) dissociation, contrary to previous proposals. We also found that the Ci binding partner, Costal 2 (Cos2), which silences Ci in the absence of Hh, can facilitate Ci activation by Fu kinase.
Composing egocentric and allocentric maps for flexible navigation
Authors: Shani Daniel , Dayan Peter 
Published: January 2025

AbstractEgocentric representations of the environment have historically been relegated to being used only for simple forms of spatial behaviour such as stimulus-response learning. However, in the many cases that critical aspects of policies are best defined relative to the self, egocentric representations can be advantageous. Furthermore, there is evidence that forms of egocentric representation might exist in the wider hippocampal formation. Nevertheless, egocentric representations have yet to be fully incorporated as a component of modern navigational methods. Here we investigate egocentric successor representations (SRs) and their combination with allocentric representations. We build a reinforcement learning agent that combines an egocentric SR with a conventional allocentric SR to navigate complex 2D environments. We demonstrate that the agent learns generalisable egocentric and allocentric value functions which, even when only additively composed, allow it to learn policies efficiently and to adapt to new environments quickly. Our work shows the benefit for the hippocampal formation to capture egocentric, as well as allocentric, relational structure – and we link the egocentric SR to findings in the lateral entorhinal cortex. We offer a new perspective on how cognitive maps could usefully be composed from multiple simple maps representing associations between state features defined in different reference frames.
An Evolutionary Novelty in TRPV1 Functional Regulation: Characterization of a Dominant-Negative Isoform Exclusive to Catarrhine Primates
Authors: Cancino Sofía , Cabeza-Bratesco Deny , Matamala Ella , Jork Julia M. , Zavala Kattina , Montecinos Cristian , Bustamante-Villarreal Sebastián , Maldonado Rodrigo , Riadi Gonzalo , Brauchi Sebastian E. , Opazo Juan C. 
Published: January 2025

AbstractTRPV1, a member of the transient receptor potential (TRP) family, is a non-selective cation channel primarily known for its role in pain perception, inflammation, and thermosensation. In mammals, it responds to noxious heat (>43°C) and chemical stimuli such as capsaicin and protons. It is widely expressed in sensory neurons, notably in the dorsal root and trigeminal ganglia. However, it is also found in some non-neuronal tissues, like the skin and bladder. The human canonical variant of TRPV1 renders a protein with 839 residues. Different splice variants have been described, and some display a dominant negative effect, partially or totally inhibiting the activity of the canonical counterpart. Here, we characterize a splice variant that encodes for a channel of 850 amino acids (TRPV1850). This variant is an evolutionary novelty of catarrhine (Old World monkeys and apes) primates, incorporating an exon of 33 bp long. Both imaging of membrane expression and electrophysiological recordings suggest that TRPV1850alone does not reach the plasma membrane. However, in cells co-expressing the canonical and TRPV1850variants, the latter would act as a dominant negative, preventing the canonical variant from reaching the plasma membrane and rendering smaller macroscopic currents in response to capsaicin. Thus, this new isoform of the TRPV1 ion channel represents a novel form of functional regulation only present in apes and Old World monkeys.SignificanceWe identified a novel isoform of the TRPV1 ion channel, a protein essential for detecting pain and heat stimuli unique to apes and Old World monkeys. This isoform contains an additional exonic sequence encoding eleven amino acids, which originated in the common ancestor of apes and Old World monkeys. This additional exonic sequence disrupts a specific domain in the N-terminal region of the protein, resulting in functional differences. Unlike the canonical TRPV1 isoform, this variant cannot reach the plasma membrane independently. Instead, it exerts a dominant-negative effect by interfering with the canonical TRPV1 protein, reducing its ability to localize to the cell surface and diminishing its responsiveness to capsaicin, a well-known TRPV1 activator.
Intentions to choose LGBTQ+ friendly hotels: self-concepts and branding approaches
Authors: Ro Heejung 
Published: January 2025

Purpose
This study aims to examine the intentions of sexual/gender minority travelers to choose LGBTQ+ friendly hotels based on their self-concepts and branding approaches.

Design/methodology/approach
An online survey is conducted, and 295 participants are recruited through a consumer panels firm. Hypotheses are tested through a mixed ANOVA.

Findings
Sexual orientation/gender identity openness and collective self-esteem increase the intentions to choose LGBTQ+ friendly hotels. Also, the intentions are higher for a hotel with a well-known LGBTQ+ friendly reputation than for a hotel with LGBTQ+ friendly advertising and promotions; however, there is no difference found between traveling domestically and traveling abroad.

Originality/value
This research demonstrates that the intentions of sexual/gender minority travelers’ to choose LGBTQ+ friendly hotels are driven by collective self-esteem, while sexual/gender openness and branding approaches play a smaller role.
Corporate social responsibility and tax avoidance: evidence from BRICS countries
Authors: Pipatnarapong Jirarat , Beelitz Annika , Jaafar Aziz 
Published: January 2025

Purpose
Using listed firms domiciled in the founding BRICS countries, i.e. Brazil, Russia, India, China and South Africa, this study empirically examines the impact of corporate social responsibility (CSR) engagement on the degree of tax avoidance.

Design/methodology/approach
Data used in this study is sourced from the EIKON database, where CSR variables, i.e. the scores of social and environmental pillars, are extracted from ASSET4, and accounting variables are sourced from Worldscope. The authors use a series of fixed effects regression models as the baseline approach to test the hypotheses. In addition, the 2SLS regression model is used to address endogeneity issues.

Findings
Their results show that firms domiciled in BRICS countries do not use CSR strategically as “a tool” to legitimate themselves, manage their risks or minimize public scrutiny from their tax avoidance behavior, but that they develop a culture of tax compliance and CSR engagement as a complementary strategy, promising ethical conduct to external audiences and committing to serving the interests of all stakeholders.

Originality/value
This study incrementally contributes to the extant literature on the link between tax avoidance and CSR engagement by offering evidence from dominant emerging markets, where the institutional factors differ considerably from those of developed countries. Furthermore, they provide essential insights for policymakers that including responsible tax payment as part of the global CSR agenda may motivate firms to align their behaviors to tax payment.
Two-dimensional steady-state thermal analytical model of permanent magnet linear motor in Cartesian coordinates
Authors: Du Yunlu , Huang Yunkai , Guo Baocheng , Djelloul-Khedda Zakarya , Dubas Frédéric , Igarashi Hajime 
Published: January 2025

Purpose
Compared with the time-consuming numerical method and the complex lumped parameter thermal network method to solve the steady-state heat distribution of the permanent magnet (PM) linear motor, there is no analytical method based on the thermal partial differential equations. This paper aims to propose a two-dimensional (2-D) analytical model for predicting the steady-state temperature distribution of PM linear motors to improve the prediction accuracy and speed up the calculation.

Design/methodology/approach
Based on the complex Fourier series theory and Cauchy’s product theorem, this paper presents for the first time a general analytical solution for 2-D temperature field in Cartesian coordinates. Then, by combining the electromagnetic field finite element model (FEM), the copper loss, iron loss and PM eddy current loss are used as the heat sources of the thermal analytical model. Finally, the solution to the temperature field is obtained by solving the system equations under boundary and interface conditions.

Findings
The analytical results are in good agreement with those from the thermal FEM, and the calculation speed is significantly faster than that of the thermal FEM.

Originality/value
The multilayer model proposed in this paper can consider heat conduction, convection and radiation. It is not only suitable for PM linear motors but also has significant application value for the thermal analysis of electromagnetic devices modeled in 2-D Cartesian coordinates.
Cloud accounting: a systematic literature review
Authors: Vo Van Hien , Abu Afifa Malik , Nguyen Nha , Bui Duong Van 
Published: January 2025

Purpose
Cloud accounting has emerged as a compelling area of research, experiencing significant growth in recent years. Therefore, this paper seeks to analyze the dispersed academic literature on cloud accounting research. Through this review, this study aims to gain insights into various aspects including research methodologies, frameworks, geographical dispersion, trends, pivotal factors and causal relationships pertinent to cloud accounting.

Design/methodology/approach
To offer a comprehensive and objective overview of cloud accounting research, this study adopts a systematic literature review approach. Seventy-five papers published between 2013 and 2023 were selected from the Scopus database, Springer Link, Emerald Insight and IEEE Xplore, following specific criteria outlined in the PRISMA process.

Findings
Analysis reveals that cloud accounting research is organized into four primary themes: understanding the benefits and challenges of cloud accounting; identifying factors influencing cloud accounting adoption; exploring factors influenced by cloud accounting implementation; and examining the mediating role of cloud accounting. Besides, the analysis indicates a notable surge in cloud accounting research from 2021 to 2023, with 40 articles published during this period, signaling a growing interest in the topic. Previous studies primarily concentrated on developing countries, with Jordan, Indonesia, Malaysia, India and Nigeria emerging as focal points. The organizational level was predominantly explored, particularly in the context of small- and medium-sized enterprises, indicating a trend toward organizational-level analysis. Notably, a significant portion of cloud accounting studies were not published in prestigious journals indexed under Scopus.

Originality/value
This study provides insights into the publication status of cloud accounting by year, journal and country. Methodological, theoretical and unit of analysis information is also presented. Finally, the study outlines limitations and suggests future research directions.
Linking talent management, work engagement and sustainable organizational performance: the mediating role of perceived supervisor support and perceived organizational support
Authors: Karim Rashed Al , Jebunnesa Ummea , Rabiul Md Karim 
Published: January 2025

Purpose
This study aims to examine how perceived supervisor and organizational support mediate the association between talent management and work engagement in Bangladesh’s manufacturing industry. It also analyzes the association between work engagement and sustainable organizational performance.

Design/methodology/approach
A structured survey questionnaire was used to collect data from 385 employees working in the manufacturing industry in the Chattogram division, applying a convenience sampling approach. Data was assessed using SmartPLS software.

Findings
The key findings are that perceived supervisor and organizational support mediate the relationship between talent management practices and work engagement. Moreover, work engagement positively influences an organization’s sustainable performance.

Practical implications
This study adds knowledge to the existing literature on talent management, work engagement and sustainable organizational performance. The findings may improve employee work engagement through talent management practices in Bangladesh’s manufacturing industry.

Originality/value
This study is one of the few in the Bangladeshi manufacturing industry that provides an extensive overview of talent management practices and work engagement, using perceived supervisor and organizational support as mediating variables.
Social media mavens’ influence on consumer perceptions of cosmetic surgery: a sentiment analysis of YouTube comments
Authors: Sinha Neena , Muskaan Muskaan 
Published: January 2025

Purpose
This study aims to analyze the sentiments expressed in YouTube comments on videos from top dermatology channels, focusing on how social media mavens affect consumer behavior and perceptions related to cosmetic treatments.

Design/methodology/approach
The study utilized sentiment analysis to examine 300 comments from the top five YouTube dermatology channels using NVivo 14, a qualitative data analysis software. It categorized the comments into positive, negative, neutral and mixed sentiments to understand the impact of the content on viewers.

Findings
The analysis revealed that 54% of the comments were positive, 5.7% were negative, 18.7% were neutral and 21.6% were mixed. The study found that videos from dermatology influencers significantly impact viewers, with positive comments highlighting the informative nature of the content and negative feedback suggesting areas for improvement.

Research limitations/implications
The findings provide actionable insights for marketers and practitioners in the esthetic dermatology industry, emphasizing the importance of creating high-quality, informative content that addresses viewer concerns and ethical considerations in influencer marketing.

Originality/value
This study uniquely contributes to the literature by focusing on sentiment analysis of YouTube comments related to cosmetic surgery, offering insights into the influence of dermatology content creators on consumer behavior.