bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of heat‐killed Lactiplantibacillus plantarumTWK10 on exercise performance, fatigue, and muscle growth in healthy male adults
Authors: Cheng Yi‐Chen , Lee Chia‐Chia , Lee Mon‐Chien , Hsu Han‐Yin , Lin Jin‐Seng , Huang Chi‐Chang , Watanabe Koichi 
Published: October 2023

AbstractConsumption of Lactiplantibacillus plantarum TWK10 (TWK10) has beneficial probiotic effects, improves exercise endurance performance, regulates body composition, and mitigates aging‐related problems in mice and humans. Here, we investigated the effects of heat‐killed TWK10 on exercise endurance performance, muscle weight and strength, fatigue, and body composition in a double‐blind, placebo‐controlled clinical trial. Thirty healthy males aged 20–40 years were assigned to the Control group or heat‐killed TWK10 group (TWK10‐HK) in a balanced order according to each individual's initial maximal oxygen uptake. After 6‐week administration, the exercise endurance time in the TWK10‐HK was significantly increased (p = 0.0028) compared with that in the Control group. The grip strength on the right and left hands of the subjects was significantly increased (p = 0.0002 and p = 0.0140, respectively) in the TWK10‐HK compared with that in the Control group. Administration of heat‐killed TWK10 resulted in a significant increase (p = 0.0275) in muscle weight. After 6‐week administration, serum lactate, and ammonia levels were significantly lower in the TWK10‐HK group than in the Control group during the exercise and recovery periods. These findings demonstrate that heat‐killed TWK10 has significant potential to be used as a postbiotic for humans.
M-protein diagnostics in multiple myeloma patients using ultra-sensitive targeted mass spectrometry and an off-the-shelf calibrator
Authors: Wijnands Charissa , Langerhorst Pieter , Noori Somayya , Keizer-Garritsen Jenneke , Wessels Hans J.C.T. , Gloerich Jolein , Bonifay Vincent , Caillon Hélène , Luider Theo M. , van Gool Alain J. , Dejoie Thomas , VanDuijn Martijn M. , Jacobs Joannes F.M. 
Published: October 2023

Abstract

Objectives
Minimal residual disease status in multiple myeloma is an important prognostic biomarker. Recently, personalized blood-based targeted mass spectrometry (MS-MRD) was shown to provide a sensitive and minimally invasive alternative to measure minimal residual disease. However, quantification of MS-MRD requires a unique calibrator for each patient. The use of patient-specific stable isotope labelled (SIL) peptides is relatively costly and time-consuming, thus hindering clinical implementation. Here, we introduce a simplification of MS-MRD by using an off-the-shelf calibrator.

Methods
SILuMAB-based MS-MRD was performed by spiking a monoclonal stable isotope labeled IgG, SILuMAB-K1, in the patient serum. The abundance of both M-protein-specific peptides and SILuMAB-specific peptides were monitored by mass spectrometry. The relative ratio between M-protein peptides and SILuMAB peptides allowed for M-protein quantification. We assessed linearity, sensitivity and reproducibility of SILuMAB-based MS-MRD in longitudinally collected sera from the IFM-2009 clinical trial.

Results
A linear dynamic range was achieved of over 5 log scales, allowing for M-protein quantification down to 0.001 g/L. The inter-assay CV of SILuMAB-based MS-MRD was on average 11 %. Excellent concordance between SIL- and SILuMAB-based MS-MRD was shown (R2>0.985). Additionally, signal intensity of spiked SILuMAB can be used for quality control purpose to assess system performance and incomplete SILuMAB digestion can be used as quality control for sample preparation.

Conclusions
Compared to SIL peptides, SILuMAB-based MS-MRD improves the reproducibility, turn-around-times and cost-efficacy of MS-MRD without diminishing its sensitivity and specificity. Furthermore, SILuMAB can be used as a MS-MRD quality control tool to monitor sample preparation efficacy and assay performance.
Algorithm of differential diagnosis of anemia involving laboratory medicine specialists to advance diagnostic excellence
Authors: Hevessy Zsuzsanna , Toth Gabor , Antal-Szalmas Peter , Tokes-Fuzesi Margit , Kappelmayer Janos , Karai Bettina , Ajzner Eva 
Published: October 2023

Abstract

Objectives
Anemia is a severe global public health issue. Testing practices for anemia suggest overuse of screening laboratory tests and misinterpretation of studies even in “easy-to-diagnose” underlying causes, leading to late diagnoses and missed treatment opportunities. We aimed to develop a complete and efficient algorithm for clinical pathologists and laboratory medicine physicians for the differential diagnosis of anemia.

Methods
Comprehensive literature search encompassing original articles, studies, reviews, gold standard books, and other evidence.

Results
We created a complex algorithm, primarily for clinical pathology/laboratory use, that explores all major and several rare causes of anemia in an efficient and evidence-based manner. The algorithm includes gold-standard diagnostic laboratory tests available in most clinical laboratories and indices that can be easily calculated to provide an evidence-based differential diagnosis of anemia.

Conclusions
The diagnostic strategy combines previously available diagnostic tests and protocols in an efficient order. Clinical pathologists following the algorithm can independently provide valuable diagnostic support for healthcare providers. Clinical pathologists providing complete differential diagnostic services with the proposed algorithm may create an opportunity for an advanced diagnostic service that supports diagnostic excellence and helps patients receive a timely diagnosis and early treatment opportunities.
Shear capacity of reinforced concrete beams with recycled steel fibers
Authors: Muneam Ali K. , Makki Ragheed F. 
Published: October 2023

Abstract
This study investigates the effect of using recycled steel fibers (RSFs) extracted from cut tires with dimensions of 0.8 and 40 mm in diameter and length, respectively. Different percentages of fibers were used, which are 0.5, 1, 1.5, and 2%. Ten beams with dimensions of 150 mm × 240 mm and a length of 1,700 mm were tested, two of which are control beams without stirrups and using stirrups at a distance higher than the upper limit (d/2), which is a distance of 250 mm. In the case of using steel fibers without a stirrup, it is noticed that shear resistance increases gradually, but the type of failure remains shear. When using a minimum stirrup with steel fibers, the shear resistance increased significantly by 11.1, 23.7, 33.3, and 41.4%, respectively, compared to the reference beam without steel fibers so that the increased shear capacity in the presence of the minimum stirrups and optimal ratio of steel fibers of 2% reaches 122%, compared to the reference beam without stirrup and steel fibers, as well as converting the type of failure from shear failure to pure bending failure. When using a stirrup, this effect will be greater. The presence of RSF reduces deflection at cracking load, while increasing deflection at the ultimate load. Therefore, the stiffness and ductility ratio increased with the presence of steel fibers by 41.5 and 50.3%, respectively. Also, steel fibers delayed the appearance of beam cracks and reduced their widths.
‘Syria Rises to Receive the Caliph’: Umayyad Caliphal Titles from Cordoba to Damascus
Authors: Cardoso Elsa 
Published: October 2023

Abstract
The concept and image of a court is strongly associated with its main character, the sovereign around whom court life revolves. How was the Umayyad caliph named and conceptualized in al-Andalus? Keeping this in mind, the purpose of this paper is the discussion of the Umayyad Andalusī caliphal titles. Sources repeatedly stress the Umayyad legitimacy to the caliphate as heirs of the Umayyads of Damascus. In a panegyric, even Syria rises to receive the Andalusī caliph. The contrast of these titles with those reserved for rebels will also be considered. While the caliph was seen as a sun, the darkness was associated with rebels. Official titles used for bureaucracy and metaphorical titles reproduced in official letters and panegyric poetry will be considered, which were preserved in Andalusī sources such as the Muqtabis of Ibn Ḥayyān (d. 1075) or the ʿIqd al-Farīd of Ibn ʿAbd Rabbih (d. 940). Ceremonial situations will also be considered as the figure of the caliph was central and praised in official letters and panegyric poetry.
Reducing the fear of re-injury during rehabilitation through mental imagery as a mental health strategy in sport and exercise
Authors: Lindsay Riki , McNeil Dominic , Spittle Michael 
Published: October 2023

Abstract
Returning to sport and exercise following injury requires the athlete to become more confident in the ability to gradually explore the use of the injured area in increasingly complex and challenging ways. Emotional responses, such as fear of re-injury, are a key mental health barrier to a performer’s return to sport and exercise. To navigate such psychological responses, performers need well-developed psychological strategies, like mental imagery (MI), to facilitate a successful return to pre-injury levels of sport and exercise. MI is a well-established strategy for dealing with negative symptoms associated with injury, providing a safe and less intimidating environment to practice movements that may be perceived as risky and otherwise performed within physical training due to the fear of causing further injury. This paper aims to provide sport psychologists with recommendations on how to utilize MI to reduce fear of re-injury during the rehabilitation process to successfully facilitate return to sport and exercise. Specific examples are also outlined and discussed.
Archaeology of the Late Local Landscapes of the Hualfín Valley (Catamarca, Argentina): A Political Perspective from Cerro Colorado of La Ciénaga de Abajo
Authors: Wynveldt Federico , Iucci María Emilia , Sallés Juan Manuel , Fuertes Juana 
Published: October 2023

Abstract
As seen in many examples of imperial expansions throughout history, the Inkas applied a whole range of policies strategically adapted for the different local organizations, in every corner of their empire, and local groups, in turn, were reconfigured based on the new conditions. Starting from the investigation of a group of late local landscapes of the Hualfín Valley (Department of Belén, Catamarca) in the Northwestern Argentina, from a critical perspective of the sociopolitical definitions classically given to these societies, the aim of this study consists of describing the spatial, social, and temporal dimensions of these landscapes and advancing the discussion about how local groups socially and politically organized themselves, in immediately pre-Inka times and after the incorporation of their territories into the Inka state. To this end, a brief discussion on the Late and Inka periods in Northwestern Argentina and theoretical guidelines for landscape analysis are presented. Then, we address the analysis of one of the landscapes in particular: the Cerro Colorado de La Ciénaga de Abajo and its surroundings, and we briefly analyze the cases of Asampay, Palo Blanco, and Puerta de Corral Quemado, and the regional landscape network.
Use of gamma radiation for the antioxidant improvement of saffron [Crocus sativus] petal extract
Authors: Heidarieh Marzieh , Parsaeimehr Mahnoosh , Sheikhzadeh Najmeh 
Published: October 2023

Abstract
This in vitro study was conducted to evaluate the impact of gamma-irradiation on the antioxidant activity of the methanolic extracts of Crocus sativus petals. After gamma-irradiation at 5, 10, and 35 kGy doses, Folin-Ciocalteu, aluminum chloride colorimetric methods, Fourier transform infrared spectroscopy (FTIR), and high performance liquid chromatography (HPLC) techniques were deployed to analyze the irradiated and non-irradiated extracts. The obtained results were indicative of boosted levels of phenolic and total flavonoids compounds in methanolic extracts irradiated at 5 and 10 kGy. Results of the HPLC method also revealed that gamma-irradiation, especially at 5 kGy, could increase the quantity of certain polyphenols, including chlorogenic acid, epicatechin caffeic acid, and thioxanthene, in the saffron extract. In contrast, gamma-irradiation at 35 kGy exerted certain adverse effects on polyphenols contents. In conclusion, gamma-irradiation, especially at 5 kGy, could enhance the antioxidant potential of the methanolic extract of saffron petals.
Effects of edaravone dexborneol on neurological function and serum inflammatory factor levels in patients with acute anterior circulation large vessel occlusion stroke
Authors: Hu Xiaohong , Qian Zhenhong , Chen Jianhui , Chen Mingsheng , Zhong Wenying , Shen Chaoxiong , Hu Zhizhou , Li Rongtong 
Published: October 2023

Abstract
The goal of this study is to evaluate and analyze the effects of edaravone (EDV) dexborneol on neurological function and serum inflammatory factor levels among patients with acute anterior circulation big artery blockage stroke. A total of 142 patients with acute anterior circulation large vessel occlusion (LVO) were randomly allocated to the study group (69 patients) or the control group (73 patients). In the study group, patients were treated with 37.5 mg EDV dexborneol twice a day for 10–14 days, based on the control group. The primary efficacy outcome was the National Institutes of Health Stroke Scale score change from baseline to 90 days and the proportion of modified Rankin Scale (mRS)score ≤1 at 90 days after randomization. The secondary outcome included the decrease in inflammatory factors at 14 days. The primary safety outcome was the incidence of hemorrhagic transformation assessed according to Heidelberg bleeding classification within 7 days. A higher percentage of patients with HIHSS score ≤5 at 90 days in the EDV dexcamphorol group was observed than in the control group (75.36% vs 64.38%; P = 0.015). A higher percentage of patients with mRS score ≤1 at 90 days in the EDV dexcamphorol group was observed than in the control group (63.77% vs 50.68%; P = 0.012). After treatment, the levels of IL-6 and hs-CRP were significantly lower following treatment and compared to the control group (P < 0.05). In patients receiving the EDV dexborneol group, a significantly decreased risk of radiographic intracranial hemorrhage was found compared with the control group (20.29% vs 39.73%; P = 0.0006). In conclusion, EDV dexborneol can improve the clinical outcomes of patients with acute anterior circulation LVO stroke, which can be used as an effective supplement to thrombectomy therapy.
Why does momentum depend on inertia?
Authors: Melia Fulvio 
Published: October 2023

Abstract
Momentum is characterized in terms of inertial mass for particles moving at less than the speed of light, but entirely in terms of their energy for those lacking inertia. Does this difference suggest a physically distinct origin of momentum in the two cases and, if so, what is actually being conserved in interactions involving both types of particle? In this paper, we consider a recently proposed gravitational origin for rest-mass energy to demonstrate that a single definition of momentum applies to all particles, massless or otherwise. When introduced into this description, inertial mass is merely a surrogate for the particle’s ‘free’ energy, but does not imply an origin of momentum different from that of particles without mass.
STEM Summer Camp for Girls Positively Affects Self-Efficacy
Authors: Broder E. Dale , Fetrow Kirsten J. , Murphy Shannon M. , Hoffman Jennifer L. , Tinghitella Robin M. 
Published: October 2023

Women and racially and ethnically minoritized populations are underrepresented in science, technology, engineering, and mathematics (STEM). Out-of-school time programs like summer camps can provide positive science experiences that may increase self-efficacy and awareness of STEM opportunities. Such programs often use the same high-impact practices used in K–12 classrooms including relating concepts to real-world examples, engaging students as active participants in inquiry-driven projects, and facilitating learning in a cooperative context. They additionally provide opportunities for engaging in STEM without fear of failure, offer a community of mentors, and allow families to become more involved. We designed a summer camp for middle schoolers who identified as girls, low-income, and as a minoritized race or ethnicity. We describe the design of the camp as well as the results from a simple pre- and post-camp questionnaire that examined each camper’s relationship to science, scientific self-efficacy, and interest in having a job in STEM. We found an increase in self-efficacy in camp participants, which is important because high scientific self-efficacy predicts student performance and persistence in STEM, especially for girls. We did not detect an increase in interest in pursuing a STEM job, likely because of already high values for this question on the pre-camp survey. We add to the growing body of work recognizing the potential of out-of-school time STEM programs to increase scientific self-efficacy for girls and racially minoritized students.Tweet: Summer camp for minoritized middle-school girls increases scientific self-efficacy, a characteristic that may be important for removing barriers to participation in STEM.
N4BP1 mediates RAM domain‐dependent notch signaling turnover during neocortical development
Authors: Ma Zhihua , Zeng Yi , Wang Ming , Liu Wei , Zhou Jiafeng , Wu Chao , Hou Lin , Yin Bin , Qiang Boqin , Shu Pengcheng , Peng Xiaozhong 
Published: October 2023

AbstractNotch signaling pathway activity, particularly fluctuations in the biologically active effector fragment NICD, is required for rapid and efficient dynamic regulation of proper fate decisions in stem cells. In this study, we identified NEDD4‐binding protein 1 (N4BP1), which is highly expressed in the developing mouse cerebral cortex, as a negative modulator of Notch signaling dynamics in neural progenitor cells. Intriguingly, N4BP1 regulated NICD stability specifically after Notch1 S3 cleavage through ubiquitin‐mediated degradation that depended on its RAM domain, not its PEST domain, as had been extensively and previously described. The CoCUN domain in N4BP1, particularly the “Phe‐Pro” motif (862/863 amino acid), was indispensable for mediating NICD degradation. The Ring family E3 ligase Trim21 was, in contrast to other NEDD4 family members, required for N4BP1‐regulated NICD degradation. Overexpression of N4BP1 in cortical neural progenitors promoted neural stem cell differentiation, whereas neural progenitor cells lacking N4BP1 were sensitized to Notch signaling, resulting in the maintenance of stem‐like properties in neural progenitor cells and lower production of cortical neurons.
UBR‐1 ubiquitin ligase regulates the balance between GABAergic and glutamatergic signaling
Authors: Li Yi , Chitturi Jyothsna , Yu Bin , Zhang Yongning , Wu Jing , Ti Panpan , Hung Wesley , Zhen Mei , Gao Shangbang 
Published: October 2023

AbstractExcitation/inhibition (E/I) balance is carefully maintained by the nervous system. The neurotransmitter GABA has been reported to be co‐released with its sole precursor, the neurotransmitter glutamate. The genetic and circuitry mechanisms to establish the balance between GABAergic and glutamatergic signaling have not been fully elucidated. Caenorhabditis elegans DVB is an excitatory GABAergic motoneuron that drives the expulsion step in the defecation motor program. We show here that in addition to UNC‐47, the vesicular GABA transporter, DVB also expresses EAT‐4, a vesicular glutamate transporter. UBR‐1, a conserved ubiquitin ligase, regulates DVB activity by suppressing a bidirectional inhibitory glutamate signaling. Loss of UBR‐1 impairs DVB Ca2+ activity and expulsion frequency. These impairments are fully compensated by the knockdown of EAT‐4 in DVB. Further, glutamate‐gated chloride channels GLC‐3 and GLC‐2/4 receive DVB's glutamate signals to inhibit DVB and enteric muscle activity, respectively. These results implicate an intrinsic cellular mechanism that promotes the inherent asymmetric neural activity. We propose that elevated glutamate in ubr‐1 mutants, being the cause of the E/I shift, potentially contributes to Johanson Blizzard syndrome.
Lysosomes in senescence and aging
Authors: Tan Jay Xiaojun , Finkel Toren 
Published: October 2023

AbstractDysfunction of lysosomes, the primary hydrolytic organelles in animal cells, is frequently associated with aging and age‐related diseases. At the cellular level, lysosomal dysfunction is strongly linked to cellular senescence or the induction of cell death pathways. However, the precise mechanisms by which lysosomal dysfunction participates in these various cellular or organismal phenotypes have remained elusive. The ability of lysosomes to degrade diverse macromolecules including damaged proteins and organelles puts lysosomes at the center of multiple cellular stress responses. Lysosomal activity is tightly regulated by many coordinated cellular processes including pathways that function inside and outside of the organelle. Here, we collectively classify these coordinated pathways as the lysosomal processing and adaptation system (LYPAS). We review evidence that the LYPAS is upregulated by diverse cellular stresses, its adaptability regulates senescence and cell death decisions, and it can form the basis for therapeutic manipulation for a wide range of age‐related diseases and potentially for aging itself.
Diagnostic pitfalls in a young adult with new diabetes
Authors: Below Natalie , Morrison Deborah , McGowan Ruth , Jones Gregory C 
Published: October 2023

Summary
A 20-year-old South Asian male presented with polyuria, polydipsia, HbA1c 81 mmol/mol, BMI 28.8 and family history of both type 1 and type 2 diabetes mellitus. As autoantibody testing was negative and c-peptide level demonstrated significant endogenous insulin secretion, type 1 diabetes was excluded. Given his age and family history, the differential diagnosis included maturity-onset diabetes of the young (MODY), a rare form of diabetes caused by a single-gene variant. A high probability of MODY was calculated and he was subsequently referred for genetic testing. Although a useful tool, the pre-test probability calculator for MODY is only validated in White Europeans. A heterogenous variant of unknown clinical significance of the NEUROD1 gene was detected, leading to gliclazide use with poor response. The patient responded well to metformin. Type 2 diabetes was considered the most likely diagnosis. This case highlights the diagnostic challenges in young patients of Asian ethnicity and the importance of interpreting genetic results of unknown significance within the clinical context. Ethnicity-specific BMI thresholds should be used when classifying patients as overweight or obese.

Learning points

Variants of unknown significance detected by genetic sequencing should be interpreted within the context of the patient’s other clinical parameters.

It is important to use ethnicity-specific BMI thresholds for obesity.

Diagnosis of type 2 diabetes mellitus at younger ages is becoming increasingly common.

The pre-test probability calculator for MODY is only validated in White Europeans; although a useful guide, results should be interpreted with caution in patients of other ethnicities.