bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome profiling reveals the regulatory mechanisms of ascorbic acid and flavonoid synthesis and metabolic processes in fruit development of Ribes nigrum
Authors: Gang Huixin , Zhang Xuelin , Zhang Danni , Huo Junwei , Qin Dong 
Published: October 2023

Abstract
Background
The blackcurrant (Ribes nigrum L.) is rich in nutritional value. It is rich in ascorbic acid and flavonoids with excellent antioxidant properties. Fruit quality is an important factor affecting subsequent processing and production, yet the molecular mechanisms underlying the transcriptional regulation of blackcurrant fruit quality are largely unknown.
Results
In the current study, comparative transcriptome analysis investigated the similarities and differences between two blackcurrant varieties: 'Adelinia' and 'Heifeng' at four fruit developmental stages (young, expansion, veraison, and ripe fruit). Additionally, we observed variations in a number of physiological indicators during the growth of blackcurrant fruits. The results indicate that, during the development of blackcurrant fruits, ascorbic acid content continued to decrease, whereas flavonoid content was lowest in the veraison period. The fruit size of 'Adelinia' variety was larger than that of 'Heifeng' variety throughout the developmental. Based on the results of the transcriptome sequencing data, a total of 4295 up-regulated genes and 3529 down-regulated genes were obtained between 'Adelinia' and 'Heifeng' varieties; A total of 6,349 up-regulated and 5,770 down-regulated genes were identified between the four developmental periods, respectively. The expression of 4 genes, PMM, APX, GME, and PGI has a strong correlation with AsA content. while two APX, two AO, and one MDHAR genes showed a strong negative correlation with AsA content. A total of 19 genes related to flavonoid biosynthesis were screened. The KEGG pathway enrichment analysis showed that many DEGs were enriched in the plant hormone signal transduction (ko04075) pathway and the photosynthesis-antenna proteins (ko00196) pathway.
Conclusions
We predicted that the PMM, APX, GME, and PGI genes, which are positively correlated with ascorbic acid, may play an important role in the biosynthesis of AsA; while the negatively correlated APX, AO, and MDHAR genes may be critical for AsA in the recycling pathway. DFR, ANR, CHI, CHS, 4CL, and LAR genes are important regulators of flavonoid synthesis. Also predicted the regulatory influences of various plant hormones on the fruit size of blackcurrants. We provide genetic resources for improving fruit quality and size as well as for rationalizing the use of plant growth regulators to improve fruit quality.
Phenotypic and genotypic characterization of multidrug resistant (MDR) and extended-spectrum β-lactamase (ESBL)-producing Enterobacterales isolated from clinical samples in the Western region in Cameroon
Authors: Nkengkana Omer Aurelle , Founou Raspail Carrel , Founou Luria Leslie , Dimani Brice Davy , Koudoum Patrice Landry , Zemtsa Jessica Ravalona , Mbossi Aurelia , Mawout Cyrielle Signe , Tegang Larissa Tchakounte , Noubom Michel 
Published: October 2023

Abstract
Background: The 2017 World Health Organization (WHO) report has listed extended spectrum β-lactamases producing Enterobacterales (ESBL-PE) as critical pathogens for public health and requiring urgently new antibiotics. The aim of this study was to characterize phenotypically and genotypically ESBL-PE isolated among clinical samples in Dschang, Cameroon.
Methods: A cross-sectional study was conducted during a four-month periods from February to May 2022 in the two biggest hospitals of Dschang. Clinical samples were collected and cultured on Eosin Methylene Blue agar. Suspected growing colonies were biochemically identified using the Enterosystem Kit 18R. Antimicrobial susceptibility testing was done using the Kirby Bauer disc diffusion method and interpretated according to the CA-SFM recommendations. ESBL phenotypes were double screened using CHROMagarTM ESBL and double disk synergy test (DDST). The detection of resistance genes was performed using conventional and multiplex PCR methods. Results were analysed with SPSS (version 21) and a p-value <0.05 was considered statistically significant.
Results: A total of 597 clinical samples including urine, blood, cervico-vaginal and urethral swabs and wound samples were analyzed and 152 Enterobacterales where isolated among the 124 positives patients to Enterobacterales. The overall prevalence of ESBL-Enterobacterales was 29.61% (45/152). The most represented ESBL species were Escherichia coli (n=23; 51.11%), Klebsiella pneumoniae (n=8; 17.78%) and Citrobacter freundii (n=6; 13.33%).
Antimicrobial resistance profile of ESBL-E. coli shows 56.52% and 86.96% resistance for imipenem and ceftazidime respectively. The most common genes detected among ESBL-Enterobacterales were blaCTX-M (n=38; 84.44%) and blaTEM (n= 33; 73.33%) respectively.
Conclusion: ESBL-PE is an important public health concern in the Western region of Cameroon urging the implementation of real-time molecular epidemiological surveillance system in both hospitals and community settings combined with strict compliance of the National Action Plan on antimicrobial resistance.
Nanoemulsion encapsulation of polyphenols from honeybee pollen increases quercetin permeability in wt-MDCK monolayers
Authors: Bridi Raquel , González Pablo M , Núñez Gabriel , Montenegro Gloria , Atala Elias , de Camargo Adriano Costa 
Published: October 2023

Abstract
Honeybee pollen (HBP) is a bee product composed of flower pollen collected from diverse plant species. Traditionally, HBP has been used in both healing and human nutrition for its wide range of secondary plant metabolites, including phenolic compounds. Yet, while these natural compounds are currently arousing attention due to their beneficial health effects, they have a limited ability to cross biological barriers and hence present low bioavailability. Quercetin was proposed as a phenolic marker for quality control in Chilean HBP. This study determined the effects of encapsulation in a multiple W/O/W nanoemulsion by using epithelial MDCK-C7 cells as a permeability model. The results indicate that the multiple nanoemulsion enhanced the in vitro permeability of encapsulated quercetin. The apparent permeability (Papp) of quercetin in cells treated with the HBP-loaded multiple nanoemulsion (HBP-MNE) was 19.1 ± 0.92 x 10− 6 cm/s, which was significantly higher than that of the free HBP extract (9.28 ± 0.47 x 10− 6 cm/s), suggesting that the nanoencapsulation procedure enabled quercetin flux through the epithelium. Our findings showed that the multiple nanoemulsion improved the permeability of encapsulated quercetin in vitro. Further research in animal models or human studies is now encouraged.
Effect of the postbiotic produced by Bacillus mojavensis from pulque on colon cancer cells
Authors: Trujillo-Lopéz María Alejandra , Garduño-Vargas Miguel Ángel , Bautista-Rodriguez Elizabeth , Muñoz-Olivos Cristina , Martínez-Ortiz Verónica Miroslava , Pérez-Armendáriz Beatriz , El-Kassis Elie Girgis 
Published: October 2023

Abstract
Probiotics have shown their potential antiproliferative effect on colon cancer cells. Although studies suggest that the effect is due to microbiome regulation, it also could be caused by metabolites produced by probiotics. Therefore, in the present work, we evaluated the effect of a cell-free extract called postbiotic from B. mojavensis, a strain isolated from Pulque (a traditional Mexican drink); the viability was determined by the MTT and crystal violet assays and the migration using wound-healing assay on the colon cancer cell SW-480. In addition, the effect on Human Peripheral Blood Mononuclear Cells (PBMCs) obtained from healthy patients was determined. The results showed that 25 or 50 µg/ml of B. mojavensis decreased viability in more than 75% of SW-480 cells and migration after 24h. Moreover, 25 or 50 µg/ml of B. mojavensis showed the ability to inhibit LPS-induced proliferation by 10ug/mL. In addition, interestingly, the effect is specific for colon cancer cells since B. mojavensis did not induce a cytotoxic effect on healthy PBMCs cells. The reported results show that the postbiotic produced by the studied strain of B. mojavensis isolated from pulque has excellent potential use as an adjuvant in treating colorectal cancer.
Neuropsychiatric profiles of children with Sydenham's Chorea in West Scotland
Authors: Mushet Nadine , Morton Michael , Minnis Helen , Gillberg Christopher 
Published: October 2023

Abstract
Sydenham’s chorea (SC): a post-streptococcal neuropsychiatric disorder, the most common cause of chorea in children worldwide but rare in Europe, may be the only manifestation of acute rheumatic fever. Descriptions of symptoms at SC onset, relapses, lifetime prevalence of developmental and psychiatric disorders and services used were obtained from children and their parents in West Scotland Liaison Psychiatry and Paediatric Neurology services over a three-year period. Interviews established medical and family history of physical and psychiatric symptoms, alongside a semi-structured, psychiatric interview (KSADS- PL), exploring current and most severe past episode of psychopathology. Twelve children, 7 girls, 5 boys, interviewed, age 10–15. Findings: six pre-existing neuro-developmental problems, four pre-morbid separation difficulties; debilitating conditions at onset included wheelchair requirements, speech impairments joint pain and psychiatric disorder. Psychopathology most severe during first episode but 6/12 reported SC relapses. Diagnoses identified: separation anxiety generalised anxiety ADHD, tics, ASD, psychosis). Educational problems were frequent. SC diagnosis was sometimes delayed. Services used: cardiology, rheumatology, physiotherapy, occupational therapy, speech therapy. Medication: long-term penicillin sodium valproate also various psychotropics. SC complicated by psychiatric symptoms may be associated with pre-existing vulnerabilities and can present clinical challenges for paediatric and mental health services.
Measuring damage anisotropy in concrete from ultrasound velocity data
Authors: Fedoroff Alexis , Calonius Kim , Sklodowska Anna 
Published: October 2023

Abstract
The uniaxial compression of an initially isotropic concrete specimen induces a damage pattern that is anisotropic: in the direction of loading the concrete undergoes compaction, while in the directions orthogonal to the loading direction concrete cracking can be observed. In order to quantify such damage patterns, one possibility is to observe the changes in the stiffness tensor of a compressed specimen with respect to the stiffness tensor of the corresponding virgin state specimen.
The eigensolutions of the Kelvin-Christoffel matrix for anisotropic media provide the relation between ultrasound wave velocities in given wavefront and polarization directions and the stiffness matrix components. In this study, it is shown how using the pitch-catch method for the determination of first time arrival, one can evaluate the sound wave velocities in different directions and compute the stiffness tensor components for a damaged concrete specimen. Plots of stiffness tensor components as a function of non-recoverable strain gives a qualitative measure of the anisotropic strain degradation process.
Factors influencing Risky Sexual Behavior: A study of female Undergraduate Students of University of Lagos, Akoka, Nigeria
Authors: Abdulganiyu Abdulazeez 
Published: October 2023

Abstract
Background
Out of 1.9 million people living with HIV in 2018, about 1.5% were within age 15–49 years although efforts have been made to limit the spread of HIV/AIDS in the country but it still retains an upward trajectory in certain states due to engagement of youths in risky sexual behaviours Thus, this study examined factors influencing risky sexual behaviors among female Undergraduate Students of University of Lagos, Akoka.
Methods
A quantitative methodological approach was adopted for this study. The study location was the University of Lagos in Nigeria. A total number of 400 sample size was considered for this study. The multi-stage sampling technique will be applied. The questionnaire was used in the process of gathering data for this study. Frequency count and percentage, including valid percentage and cumulative percentage, were used.
Results
The findings of the study showed that a significant majority of respondents 367 respondents, (91.7%) have had sexual intercourse. Peer influence played a role in the first sexual activity for the majority of respondents, with 288 respondents (78.5%) indicating that their first sexual experience was influenced by their peers. The study also revealed that (62.2%) of respondents reported engaging in unprotected mouth-to-genital contact.
Conclusions
The vast majority of students acknowledged having had sexual activities. Notably, peer influence emerged as a crucial component of their first sexual encounters, demonstrating the influence of their peers on their choices. Friends persisted in having a big impact on the respondents' subsequent sexual behavior. Recommendations such as government implementing comprehensive sex education programs that emphasize healthy relationships, consent, and responsible decision-making, aiming to empower young individuals with accurate information and skills to resist negative peer pressure were made in tandem with the findings of this study.
Rapid detection of multiple phytoplasmas with an All-In-One Dual (AIOD) CRISPR assay
Authors: Feng Jiancan , Chen Peng , Li Qicheng , Jiao Jian , Yang Qiqi , Guo Sicheng , Yao Jia , Chen Yun , Ye Xia , Cheng Jun , Tan Bin , Zheng Xianbo , Wu Yingxia , Cai Hong , Wu Yunfeng , Wang Huiyu , Li Jidong 
Published: October 2023

Abstract

Phytoplasma can infect thousands of plants and caused huge economic losses around the world. The large-scale spread and serious lethality of phytoplasma prompt the urgent need for sensitive, accurate, visual and rapid detection of these pathogens. Current molecular assays used for detecting phytoplasma are expensive and time consuming. Here, we established a novel All-In-One Dual (AIOD) CRISPR detection platform based on CRISPR/LbCas12a technology for the diagnosis of multiple phytoplasma. The protocol is simple, requiring one vessel, rapid and sensitive, and the output is visual. Cas12a/crRNAs complexes are added into a reaction containing RPA Mix, RPA primers and single-stranded DNA fluorophore-quencher (ssDNA-FQ). All components, including 1 μL of sample DNA, are added together and then incubated in one tube at 37 °C. Phytoplasma was detected after 15 min from leaf harvest. Positive results can be observed by the naked eye via fluorescent signals. We optimized the amounts of crRNA, LbCas12a and the ssDNA fluorophore in the detection system. Finally, an optimized system was established containing 1,000 nM ssDNA-FQ and a 2:1:1 ratio of LbCas12a/crRNA1/crRNA2 complex with a 0.8 μM concentration as 1. In the optimized reaction, the AIOD-CRISPR detection system exhibited high sensitivity, with limits of detection reaching 3.37E + 2 copies of phytoplasma DNA per reaction. Field tests indicated the AIOD-CRISPR detection system possessed high specificity and reached the 100% accuracy when compared with PCR detection. In conclusion, the AIOD-CRISPR detection system is an ideal selection with high specificity and sensitivity for phytoplasma detection.
Systematic Review and Meta-analysis on the Effect of Adjuvant Gonadotropin-releasing Hormone Agonist (GnRH-a) on Pregnancy Outcomes in Women with Endometriosis Following Conservative Surgery.
Authors: Qing Xuemei , He Lele , Ma Ying , Zhang Yong , Zheng Wenxin 
Published: October 2023

Abstract
Background
Endometriosis frequently results in pain and infertility. While conservative surgery offers some relief, it often falls short of ensuring satisfactory pregnancy outcomes. Adjuvant GnRH-a is administered post-surgery to mitigate recurrence; however, its impact on pregnancy outcomes remains debated. This study endeavors to assess the efficacy of adjuvant GnRH-a in enhancing pregnancy outcomes post-conservative surgery in endometriosis patients.
Methods
Databases including PubMed, Embase, the Cochrane Library, Medline (Ovid), Web of Science, and Scopus were rigorously searched up to 02 August 2023, without linguistic constraints. Identified articles were screened using strict inclusion and exclusion criteria. Evaluated outcomes encompassed pregnancy rate, live birth rate, miscarriage rate, ectopic pregnancy rate, multiple pregnancy rate, time from surgery to pregnancy, recurrence rate, and adverse reaction rate. The Cochrane risk of bias tool and the Jadad score evaluated the included studies' quality. Subgroup and sensitivity analysis were implemented to analyze the pooled results. A meta-analysis model expressed results as standardized mean difference (SMD) and Risk ratio (RR).
Results
A total of 17 studies about 2485 patients were assimilated. Meta-analysis revealed that post-surgery, the GnRH-a cohort experienced a marginally elevated pregnancy rate (RR = 1.20, 95% CI = 1.02–1.41; P = 0.03) and a reduced mean time to conceive (RR = -1.17, 95% CI = -1.70- -0.64; P < 0.0001). Contrarily, other evaluated outcomes did not exhibit notable statistical differences.
Conclusions
Incorporating adjuvant GnRH-a following conservative surgery may be deemed beneficial for women with endometriosis, especially before Assisted Reproductive Technology (ART). Nonetheless, owing to pronounced heterogeneity, subsequent research is warranted to substantiate these potential advantages conclusively.
Registration number:
CRD42023448280.
Hypertension Management and Health Belief in Middle-Aged Laotian Population
Authors: Kang Younhee , Kim Boeun 
Published: October 2023

Abstract
Background
This study estimated prevalence of hypertension awareness, treatment, and control and examined their associations with health locus of control by rural and urban areas.
Methods
This is cross-sectional study using population-based data. Middle-aged people living in central Laos were recruited using a multistage cluster sampling approach and analytic sample size was 924. Blood pressure was measured by a digital automatic blood pressure monitor. Self-reported hypertension awareness, treatment, and control were recorded and health locus of control was assessed using the Multidimensional Health Locus of Control Scale (HLOC).
Results
Proportions of awareness, treatment, and control were 43.4%, 42.3%, and 17.8%. In urban areas, individuals with one point greater in doctor HLOC were 15% more likely to be aware of their hypertension status (95% CI: 1.03–1.28), 13% more likely to adhere to blood pressure-lowering medication (95% CI: 1.01–1.27), and 25% more likely to have controlled hypertension (95% CI: 1.11–1.41). In rural areas, individuals with one point higher in internal HLOC was related to 17% higher chance of having controlled hypertension (95% CI: 1.05–1.29).
Conclusions
The findings call for actions to prevent and manage hypertension and health beliefs should be considered to design a culturally tailored intervention in Laos.
Is Rural Non-farm Employment Distress Driven in Uttar Pradesh? Evidence from the NSSO rounds and PLF Survey
Authors: Hashmi Shadab 
Published: October 2023

Abstract
Using National Sample Survey Organization (NSSO) data, this study assessed the employment situation in Uttar Pradesh, with a focus on non-farm employment. The primary objective of our study is to investigate the potential causes of employment growth in non-farm activities over time. In Uttar Pradesh, we observed that a higher proportion of the casual workforce and stagnation of rural manufacturing activities, as well as a declining proportion of people who were self-employed and regularly employed, contributed to the casualization of the rural workforce. This suggests that the rural workforce was forced to shift from agricultural to non-farm activities as a result of distress. The key finding is that self-employment and regular employment outside agriculture seemed to be high-return activities, whereas casual employment outside agriculture was linked to low-return activities. Using a multinomial logit model, the study revealed that across all employment status categories, including agricultural labor, cultivators, non-farm regular employment, non-farm self-employment, and non-farm casual employment, the likelihood of working in high-paying non-farm regular and self-employment activities increases with better educational attainment, larger landholdings, and placement among the upper castes in social groups.
JEL Code: J22, J24, J46
Efficient nanostructured materials to reduce nutrient leaching to overcome environmental contaminations
Authors: Nadeem Farwa , Hanif Muhammad Asif , AlMasoud Najla , Alomar Taghrid S. , Younis Adnan 
Published: October 2023

Abstract
Nutrient leaching is a major reason for fresh and ground water contamination. Menthol is the major bioactive ingredient of Mentha arvensis L. and one of the most traded products of the global essential oil market. The indigenous production of menthol crystals in the developing countries of the world can prove to be the backbone for the local growers and poor farmers. Therefore, present research was designed to check the effects of nanostructured plant growth regulators (PGRs) (28-homobrassinolide and ethephon) with reduced leaching potentials on the essential oil and menthol (%) of Mentha arvensis L. The prepared nanoformulations were characterized by the Fourier transform infrared (FTIR) spectroscopy, Laser induced breakdown spectroscopy (LIBS), Differential scanning colorimetry-thermal gravimetric analysis (DSC-TGA), Scanning electron microscopy (SEM) and Atomic absorption spectrometry (AAS). The menthol (%) was determined by modified spectrophotometric and gas chromatographic (GC) method. The highest essential oil (%) was obtained by the 28-homobrassinolide-Zn-NPs-L-II (0.92 ± 0.09%) and ethephon-Ca-NPs-L-III (0.91 ± 0.05%) as compared to the control (0.65 ± 0.03%) and blank (0.62 ± 0.09%). The highest menthol (%) was obtained by the 28-homobrassinolide-Ca-NPs-L-I (80.06 ± 0.07%), 28-homobrassinolide-Ca-NPs-L-II (80.48 ± 0.09%) and 28-homobrassinolide-Ca-NPs-L-III (80.84 ± 0.11%) and ethephon-Ca-NPs-L-III (81.53 ± 0.17%) and ethephon-Zn-NPs-L-II (81.93 ± 0.26%) as compared to the control (67.19 ± 0.14%) and blank (63.93 ± 0.17%).