bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing the epidermal growth factor receptor gene with RNAi may be developed as a potential therapy for non small cell lung cancer
Authors: Zhang Min , Zhang Xin , Bai Chun-Xue , Song Xian-Rang , Chen Jie , Gao Lei , Hu Jie , Hong Qun-Ying , West Malcolm J , Wei Ming Q 
Published: June 2005

Abstract
Lung cancer has emerged as a leading cause of cancer death in the world. Non-small cell lung cancer (NSCLC) accounts for 75–80% of all lung cancers. Current therapies are ineffective, thus new approaches are needed to improve the therapeutic ratio. Double stranded RNA (dsRNA) -mediated RNA interference (RNAi) has shown promise in gene silencing, the potential of which in developing new methods for the therapy of NSCLC needs to be tested. We report here RNAi induced effective silencing of the epidermal growth factor receptor (EGFR) gene, which is over expressed in NSCLC. NSCLC cell lines A549 and SPC-A1 were transfected with sequence- specific dsRNA as well as various controls. Immune fluorescent labeling and flow cytometry were used to monitor the reduction in the production of EGFR protein. Quantitative reverse-transcriptase PCR was used to detect the level of EGFR mRNA. Cell count, colony assay, scratch assay, MTT assay in vitro and tumor growth assay in athymic nude mice in vivo were used to assess the functional effects of EGFR silencing on tumor cell growth and proliferation. Our data showed transfection of NSCLC cells with dsRNA resulted in sequence specific silencing of EGFR with 71.31% and 71.78 % decreases in EGFR protein production and 37.04% and 54.92% in mRNA transcription in A549 and SPC-A1 cells respectively. The decrease in EGFR protein production caused significant growth inhibition, i.e.: reducing the total cell numbers by 85.0% and 78.3 %, and colony forming numbers by 63.3% and 66.8%. These effects greatly retarded the migration of NSCLC cells by more than 80% both at 24 h and at 48 h, and enhanced chemo-sensitivity to cisplatin by four-fold in A549 cells and seven-fold in SPC-A1. Furthermore, dsRNA specific for EGFR inhibited tumor growth in vivo both in size by 75.06 % and in weight by 73.08 %. Our data demonstrate a new therapeutic effect of sequence specific suppression of EGFR gene expression by RNAi, enabling inhibition of tumor proliferation and growth. However, in vivo use of dsRNA for gene transfer to tumor cells would be limited because dsRNA would be quickly degraded once delivered in vivo. We thus tested a new bovine lentiviral vector and showed lentivector-mediated RNAi effects were efficient and specific. Combining RNAi with this gene delivery system may enable us to develop RNAi for silencing EGFR into an effective therapy for NSCLC.
Wearable feedback systems for rehabilitation
Authors: Sung Michael , Marci Carl , Pentland Alex 
Published: June 2005

Abstract
In this paper we describe LiveNet, a flexible wearable platform intended for long-term ambulatory health monitoring with real-time data streaming and context classification. Based on the MIT Wearable Computing Group's distributed mobile system architecture, LiveNet is a stable, accessible system that combines inexpensive, commodity hardware; a flexible sensor/peripheral interconnection bus; and a powerful, light-weight distributed sensing, classification, and inter-process communications software architecture to facilitate the development of distributed real-time multi-modal and context-aware applications. LiveNet is able to continuously monitor a wide range of physiological signals together with the user's activity and context, to develop a personalized, data-rich health profile of a user over time. We demonstrate the power and functionality of this platform by describing a number of health monitoring applications using the LiveNet system in a variety of clinical studies that are underway. Initial evaluations of these pilot experiments demonstrate the potential of using the LiveNet system for real-world applications in rehabilitation medicine.
Psychiatric hospitalisation and suicide among the very old in Denmark: Population-based register study
Authors: Erlangsen Annette , Mortensen Preben Bo , Vach Werner , Jeune Bernard 
Published: June 2005

BackgroundVery old people have higher suicide rates than the younger elderly population. Psychiatric disorders are known to have a strong association with suicide among elderly people.AimsTo analyse the suicide risk associated with psychiatric hospitalisation among the very old (⩾80 years) compared with the middle-aged (50–64 years) and old (65–79 years) populations.MethodIndividual-level data on the entire Danish population aged 50 years or over were analysed for the period 1994–1998. Relative suicide risks were calculated using event-history analysis.ResultsAmong 1 978 527 persons, 2323 died by suicide. Although the very old group exhibited a four-fold to five-fold increase in risk of suicide for those previously hospitalised, we noted an inverse interaction effect: the increase is distinctly smaller compared with that in the middle-aged and old groups.ConclusionsThe association between suicide and psychiatric hospitalisation is much weaker for the very old than for the old. Psychiatric disorders among very old people may be interacting with other disorders, may be underdiagnosed or treated in other healthcare settings.
Interventions to Enhance Physical and Psychological Functioning Among Younger Women Who Are Ending Nonhormonal Adjuvant Treatment for Early-Stage Breast Cancer
Authors: Scheier Michael F. , Helgeson Vicki S. , Schulz Richard , Colvin Suzanne , Berga Sarah , Bridges Michael W. , Knapp Judy , Gerszten Kristina , Pappert William Scott 
Published: June 2005

Purpose To conduct a clinical trial to determine if an educational intervention and a nutritional intervention could enhance physical and psychological functioning among younger women completing treatment for early-stage breast cancer. Patients and Methods Younger women (50 years of age or younger, N = 252), within 2 months of having completed active nonhormonal adjuvant therapy, diagnosed with stage 0, I, or II breast cancer with 10 or fewer positive lymph nodes were randomly assigned to a three-arm clinical trial. Women in the control arm of the trial received standard medical care. Women in the two active arms received either an educational intervention, designed to provide information about their illness and enhance adjustment, or a nutritional intervention, designed to promote a more healthy diet. Primary end points included mental functioning, physical functioning, and depressive symptoms. Women were assessed before random assignment, 4 months later (immediately postintervention), and 13 months later (9 months postintervention). Results Participants assigned to the two active treatment arms had significantly less depressive symptomatology and better physical functioning by 13-month follow-up (differences between the two active arms were nonsignificant). These effects were primarily accounted for by changes in intrusive thoughts, concerns regarding cancer recurrence and mortality, self-concept perceptions, and self-efficacy expectations. Conclusion Tailored psychosocial interventions can be effectively designed to enhance adjustment among younger women who are completing nonhormonal adjuvant therapy.
Phase I/II Study of Galiximab, an Anti-CD80 Antibody, for Relapsed or Refractory Follicular Lymphoma
Authors: Czuczman Myron S. , Thall Aron , Witzig Thomas E. , Vose Julie M. , Younes Anas , Emmanouilides Christos , Miller Thomas P. , Moore Joseph O. , Leonard John P. , Gordon Leo I. , Sweetenham John , Alkuzweny Baha , Finucane Deborah M. , Leigh Bryan R. 
Published: June 2005

PurposeThis multicenter, dose-escalation study evaluates the safety, pharmacokinetics, and efficacy of galiximab (anti-CD80 monoclonal antibody) in patients with relapsed or refractory follicular lymphoma.Patients and MethodsPatients had follicular lymphoma that had relapsed or failed to respond to primary therapy; the majority (90%) presented with stage III or IV disease. Four weekly intravenous infusions of galiximab were administered at doses of 125, 250, 375, or 500 mg/m2.ResultsThirty-seven patients received galiximab treatment and were evaluated for safety; 35 were assessable for response. Antibody infusions were safe and well tolerated with no dose-limiting toxicities. A total of 22 (60%) of 37 patients experienced adverse events related to galiximab. All but one of the events were grade 1 or 2; the most common were fatigue, nausea, and headache. Cytopenias were rare; only one patient experienced anemia and febrile neutropenia, which were unrelated to galiximab and resolved after treatment. No patient developed antigaliximab antibody formation. The mean serum half-life ranged from 13 to 24 days. The overall response rate was 11% (two complete responses and two partial responses). Time to best response was delayed (months 3, 6, 9, and 12). Twelve patients (34%) maintained stable disease. Nearly half of all patients (49%) had a decrease in indicator lesions. Two responders remain on study without progression (22 and 24.4 months).ConclusionThe favorable safety profile of galiximab and evidence of single-agent biologic activity and dose-dependent pharmacokinetics support further evaluation of galiximab as a treatment for follicular lymphoma, possibly in combination with other lymphoma therapies.
Biophysical studies of a synthetic mimic of the apoptosis‐detecting protein annexin v
Authors: Koulov Atanas V. , Hanshaw Roger G. , Stucker Kenneth A. , Lakshmi C. , Smith Bradley D. 
Published: June 2005

AbstractA Zn2+‐dipicolylamine coordination compound is shown to sense the presence of anionic phospholipids in a membrane bilayer. The sensor contains two dipicolylamine subunits attached to an anthracene scaffold, which exhibits a maximum absorbance at 380 nm, and undergoes an enhancement in fluorescence intensity when exposed to membranes enriched in phosphatidylserine. For these reasons, the compound is referred to as PSS‐380 (Phosphatidylserine Sensor, 380 nm). The fluorescence emission of PSS‐380 is enhanced up to tenfold by the presence of vesicles containing the anionic phospholipids phosphatidylserine, phosphatidylglycerol, or phosphatidic acid. No enhancement in fluorescence is observed upon exposure to vesicles containing only zwitterionic phosphatidylcholine, or exposure to monodispersed (non‐aggregated) anionic phospholipids. The sensing effect is cooperative; not only does association to the vesicles increase if the vesicles have raised levels of anionic phospholipid, but the maximum fluorescence at sensor saturation is also enhanced. It appears that sensing is triggered by the three‐component self‐assembly of sensor, Zn2+, and the anionic membrane surface, which leads to diminished photo‐induced electron transfer (PET) quenching. The utility of PSS‐380 in flow cytometry and fluorescence microscopy is demonstrated by using the molecule to detect the appearance of phosphatidylserine on the plasma membrane surface of various cell lines. Thus, PSS‐380 can identify apoptotic cells in the same way as the commonly used protein reagent annexin V.
Formation and applications of stable 10 nm to 500 nm supramolecular porphyrinic materials
Authors: Drain Charles Michael , Bazzan Giorgio , Milic Tatjana , Vinodu Mikki , Goeltz John C. 
Published: June 2005

AbstractNanoscaled materials of organic dyes are of interest for a variety of potential applications because of the rich photonic properties that this class of molecules can impart. One mode to form such nanoscaled materials is via self‐organization and self‐assembly, using reasonably well understood methods in supramolecular chemistry. But there are inherent complexities that arise from the use of organic‐based supramolecular materials, including stability toward dioxygen, structural stability, and nanoarchitectures that may change with environmental conditions. Porphyrinoids have rich photonic properties yet are remarkably stable, have a rigid core, are readily functionalized, and metalation of the macrocycle can impart a plethora of optical, electronic, and magnetic properties. While there are many <10 nm porphyrinic assemblies, which may or may not self‐organize into crystals, there is a paucity of 10–500 nm porphyrinic materials that can be isolated and stored. A variety of strategies towards the latter nanoscopic porphyrinic materials are discussed in terms of design, construction, and nanoarchitecture. The hierarchical structures include colloids, nanorods, nanotubes, nanorings, and nano‐crystalline materials. This prolegomenon emphasizes the supramolecular chemistry, structure‐stability, and structure‐function relationships. The goal herein is to examine general trends and delineate general principles.
Dynamic equilibrium of self‐assembled cyclic trimer and tetramer of 1‐methyl‐5‐imidazolylcobalt(III)porphyrin
Authors: Tanaka Akira , Ryuno Aya , Okada Saori , Satake Akiharu , Kobuke Yoshiaki 
Published: June 2005

AbstractDynamic equilibrium of self‐assembled multi‐porphyrin systems is of interest in obtaining switchable photoresponsive material, but rarely reported. 1‐methyl‐5‐imidazolylcobalt(III)porphyrin (1Co) synthesized here assembled automatically into cyclic trimer and tetramer by intermolecular imidazolyl‐cobalt(III) coordination. The trimer‐tetramer equilibrium was dependent on concentration and solvent, as examined by NMR spectrometry. In CDCl3, the tetramer formation was favored at high concentrations, as the ratio of the trimer to the tetramer was 1:2 at 14.8 mM 1Co, and shifted to 1:8 at 74 mM. Further, when the sample was concentrated from a CHCl3 solution to dryness, the ratio increased to 1:24 on dissolution. In CD3OD, on the other hand, only the trimer was observed in the wide concentration range. Accordingly, both the trimeric and the tetrameric structures could be prepared selectively by the choice of concentration and solvent.
Synthesis and characterization of fully conjugated porphyrin tapes
Authors: Ikeue Takahisa , Aratani Naoki , Osuka Atsuhiro 
Published: June 2005

Abstractmeso‐meso, ß‐ß, ß‐ß Triply‐linked zinc(II) porphyrin tapes were synthesized by powerful oxidation of meso‐meso‐linked zinc(II) porphyrin arrays up to tetramers with DDQ‐Sc(OTf)3. Coordination of butylamine to zinc(II) ions of porphyrin rings dissociates their aggregation, resulting in clear NMR spectra and sharper red‐shifted absorption bands.
Seasonal Movement and Habitat Use by Subadult Bull Trout in the Upper Flathead River System, Montana
Authors: Muhlfeld Clint C. , Marotz Brian 
Published: June 2005

Abstract
Despite the importance of large-scale habitat connectivity to the threatened bull trout Salvelinus confluentus, little is known about the life history characteristics and processes influencing natural dispersal of migratory populations. We used radiotelemetry to investigate the seasonal movements and habitat use by subadult bull trout (i.e., fish that emigrated from natal streams to the river system) tracked for varying durations from 1999 to 2002 in the upper Flathead River system in northwestern Montana. Telemetry data revealed migratory (N = 32 fish) and nonmigratory (N = 35 fish) behavior, indicating variable movement patterns in the subadult phase of bull trout life history. Most migrating subadults (84%) made rapid or incremental downriver movements (mean distance, 33 km; range, 6–129 km) to lower portions of the river system and to Flathead Lake during high spring flows and as temperatures declined in the fall and winter. Bull trout subadults used complex daytime habitat throughout the upper river system, including deep runs that contained unembedded boulder and cobble substrates, pools with large woody debris, and deep lake-influenced areas of the lower river system. Our results elucidate the importance of maintaining natural connections and a diversity of complex habitats over a large spatial scale to conserve the full expression of life history traits and processes influencing the natural dispersal of bull trout populations. Managers should seek to restore and enhance critical river corridor habitat and remove migration barriers, where possible, for recovery and management programs.
Sex differences in cell death
Authors: Li Hong , Pin Scott , Zeng Zhiyuan , Wang Michael M. , Andreasson Katrin A. , McCullough Louise D. 
Published: June 2005

AbstractFemale patients experience substantial neuroprotection after experimental stroke compared with male patients, a finding attributed to the protective effects of gonadal hormones. This study examined the response of male‐ and female‐derived organotypic hippocampal slices to oxidative and excitotoxic injury. Both oxygen and glucose deprivation and N‐methyl‐D‐aspartic acid exposure led to neuronal death; however, female‐derived cultures sustained less injury than male‐derived cultures. Cell death after oxygen and glucose deprivation was ameliorated in male cultures, but not female cultures, by the addition of 7‐nitroindazole, a neuronal nitric oxide synthase inhibitor. These studies have relevance to researchers investigating neuroprotective agents in mixed sex experiments. Ann Neurol 2005
A New Hexagonal 12‐Layer Perovskite‐Related Structure: Ba6Ln2Ti4O17 (Ln: Nd and Y).
Authors: Kuang Xiaojun , Jing Xiping , Loong Chun‐K. , Lachowski Eric E. , Skakle J. M. S. , West Anthony R. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Nucleophilic Addition to 1,2‐Dimethyl‐3‐arylsulfonyl‐4,5‐dihydroimidazolium Iodides.
Authors: Xia Chizhong , Chen Jianxin , Wang Hongxing , Kang Congmin , Zhao Bingjun , Ni Yanping , Zhou Peiwen 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Copper(I)‐Catalyzed Addition of Trifluoromethylated Benzyl Radicals Derived from Aryltrifluoroethyl Bromides to Terminal Olefins.
Authors: Okano Takashi , Sugiura Hirokazu , Fumoto Masataka , Matsubara Hiroyoshi , Kusukawa Takahiro , Fujita Makoto 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reaction of Terminal Alkynes with Hydrazines to Give Nitriles, Catalyzed by TpRuCl(PPh3)2: Novel Catalytic Transformation Involving a Vinylidene Ruthenium Intermediate.
Authors: Fukumoto Yoshiya , Dohi Toshifumi , Masaoka Hideo , Chatani Naoto , Murai Shinji 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Chiral Acrylate Equivalent for Metal‐Free Diels—Alder Reactions: endo‐2‐Acryloylisoborneol.
Authors: Palomo Claudio , Oiarbide Mikel , Garcia Jesus M. , Gonzalez Alberto , Lecumberri Ainara , Linden Anthony 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Regio‐ and Stereoselective Synthesis of Homoallylic Alcohols Based on the Use of (3‐Chloroprop‐1‐en‐1‐yl)boronates.
Authors: Lombardo Marco , Morganti Stefano , Tozzi Massimo , Trombini Claudio 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Convenient Synthesis of 2‐Amino‐1,3,3‐tricyano‐5‐nitro‐4,6‐diarylcyclohexenes Catalyzed by KF‐Alumina.
Authors: Shi Daqing , Tu Shujiang , Yao Changsheng , Wang Xiangshan , Wang Yucheng 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Biological Evaluation of 7‐Azaindolocarbazoles.
Authors: Routier Sylvain , Ayerbe Nathalie , Merour Jean‐Yves , Coudert Gerard , Bailly Christian , Pierre Alain , Pfeiffer Bruno , Caignard Daniel‐Henri , Renard Pierre 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Bis(lactone)‐Annulated 4H‐pyranes and 1,4‐Dihydropyridines from 3,3′‐(Nitrobenzylidene)‐bistetronic Acids.
Authors: Goerlitzer K. , Trittmacher J. , Bartke U. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
An Efficient One‐Pot Synthesis of 6‐Alkoxy‐8,9‐dialkylpurines via Reaction of 5‐Amino‐4‐chloro‐6‐alkylaminopyrimidines with N,N‐Dimethylalkaneamides and Alkoxide Ions.
Authors: Baraldi Pier Giovanni , Broceta Asier Unciti , Pineda de las Infantas Maria Jose , Diaz Mochum Juan Jose , Espinosa Antonio , Romagnoli Romeo 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Structure—Activitiy Relationships of 4‐Amino‐5‐chloro‐N‐ (1,4‐dialkylhexahydro‐1,4‐diazepin‐6‐yl)‐2‐methoxybenzamide Derivatives, Novel and Potent Serotonin 5‐HT3 and Dopamine D2 Receptors Dual Antagonist.
Authors: Hirokawa Yoshimi , Harada Hiroshi , Yoshikawa Takashi , Yoshida Naoyuki , Kato Shiro 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Studies Toward the Synthesis of α‐Fluorinated Phosphonates via Tin‐Mediated Cleavage of α‐Fluoro‐α‐(pyrimidin‐2‐ylsulfonyl)alkylphosphonates. Intramolecular Cyclization of the α‐Phosphonyl Radicals.
Authors: Wnuk Stanislaw F. , Bergolla Luis A. , Garcia Jr. Pedro I. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Stereoselective Synthesis of Some Novel Heterocyclic Estrone Derivatives by Intramolecular 1,3‐Dipolar Cycloaddition.
Authors: Frank Eva , Woelfing Janos , Aukszi Beatrix , Koenig Verena , Schneider Thomas R. , Schneider Gyula 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Novel Mimetics of CMP‐Sialic Acid as the Inhibitors of Sialyltransferases.
Authors: Tanaka Toru , Ozawa Machiko , Miura Tsuyoshi , Inazu Toshiyuki , Tsuji Shuichi , Kajimoto Tetsuya 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Marine Bacteria. Part 13. Phytotoxic Arylethylamides from Limnic Bacteria Using a Screening with Microalgae.
Authors: Maskey Rajendra P. , Asolkar Ratnakar N. , Kapaun Edwin , Wagner‐Doebler Irene , Laatsch Hartmut 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Solid Oxide Fuel Cells for Direct Oxidation of Liquid Hydrocarbon Fuels in Automotive Auxiliary Power Units: Sulfur Tolerance and Operation on Gasoline
Authors: Crosbie Gary M. , Murray Erica Perry , Bauer David R. , Kim Hyuk , Park Seungdoo , Vohs John M. , Gorte Raymond J. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Structural Studies with Usovites: Ba2CaMIIV2F14 (MII: Mn, Fe), Ba2CaMnFe2F14 and Ba2CaCuM2IIIF14 (MIII: Mn, Fe, Ga).
Authors: Kaiser V. , Le Lirzin A. , Darriet J. , Tressaud A. , Holler H. , Babel D. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Dinuclear Titanium(IV) Complex of p‐tert‐Butylthiacalix[4]arene as a Novel Bidentate Lewis Acid Catalyst.
Authors: Morohashi Naoya , Hattori Tetsutaro , Yokomakura Katsuya , Kabuto Chizuko , Miyano Sotaro 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reaction of Carbonyl Compounds with Trialkylsilyl Phenylselenide and Tributylstannyl Hydride under Radical Conditions.
Authors: Nishiyama Yutaka , Kajimoto Hiroyuki , Kotani Kazuya , Nishida Takuma , Sonoda Noboru 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Enhancement of Enantioselectivity by THF in Asymmetric Mo‐Catalyzed Olefin Metathesis. Catalytic Enantioselective Synthesis of Cyclic Tertiary Ethers and Spirocycles.
Authors: Teng Xin , Cefalo Dustin R. , Schrock Richard R. , Hoveyda Amir H. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Efficient Synthesis of N‐(Trifluoromethylsulfonyl)trifluoromethanesulfonimidoyl Fluoride — The Key Agent in the Preparation of Compounds with Superstrong Electron‐Withdrawing Groups and Strong Acidic Properties.
Authors: Garlyauskayte Romute Yu. , Bezdudny Andrey V. , Michot Christophe , Armand Michel , Yagupolskii Yurii L. , Yagupolskii Lev M. 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Asymmetric Synthesis of anti‐Homopropargylic Alcohols from Aldehydes and Chiral Sulfonimidoyl Substituted Bis(allyl)titanium Complexes Through Generation and Elimination of Novel Chiral Alkylidenecarbene (Dimethylamino)sulfoxonium Ylides.
Authors: Reddy Leleti Rajender , Gais Hans‐Joachim , Woo Chang‐Wan , Raabe Gerhard 
Published: June 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.