bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization and transcriptome analysis of candidate genes involved in hybrid incompatibility of Rhododendron
Authors: Xie Wei-Jia , Peng Lv-Chun , Huang Hui , Song Jie , Wang Ji-hua , Zhang Lu , Li Shi-Feng 
Published: February 2024

Abstract
One of the great unsolved mysteries of evolutionary biology concerns the genetic mechanisms underlying the reproductive isolation among diverging lineages. Hybrid incompatibility can result in reproductive isolation and speciation, showing a reduced fitness in hybrids in comparison with their parents. Therefore, identifying the candidate genes contributing to hybrid incompatibility will provide important information for uncovering the regulation mechanism of reproductive isolation. Although the genetics of hybrid incompatibility has been examined in model organisms, it has been described in relatively few instances in non-model plant. In this study, RNA-Seq was performed on the gene expression profiles of unpollinated ovary (CK) of Rhododendron delavayi as female parent, normal (T1) and aborted (T2) zygotic embryo of R. cyanocarpum and R. sinofaconeri as male parents, respectively, at 40 days after cross-pollination when the development of the two zygotic embryos were differentiated obviously. A total of 5,177 differentially expressed genes (DEGs) were identified by pairwise comparison. Venn analysis found 146 uniquely DEGs in T2-vs-T1, many of which were involved in immune responses, including one natriuretic peptide receptor 1 (NPR1), three accelerated cell death 6 (ACD6) genes and 11 disease resistance proteins. Furthermore, KEGG enrichment analysis showed that only the plant-pathogen interaction pathway were simultaneously enriched in DEGs of CK-vs-T1, CK-vs-T2, and T1-vs-T2, suggesting their important role in hybrid incompatibility. Further analysis found that 12 disease resistance protein, three nucleotide-binding site leucine-rich repeat protein (NLR), two endoplasmin homolog and one mitogen-activated protein kinase 2 (MAPK2) were included in the plant-pathogen interaction pathway. Of them, 13 genes were upregulated in aborted zygotic embryo compared with normal zygotic embryo. We inferred that NPR1, ACD6, disease resistance protein and NLR can activate autoimmune response, in turn, result in hybrid incompatibility in Rhododendron. The study provides a basis for revealing the regulation mechanism of hybrid incompatibility and could benefit genetic breeding of Rhododendron.
A machine learning model for early candidemia prediction in the intensive care unit: Clinical application
Authors: Meng Qiang , Chen Bowang , Xu Yingyuan , Zhang Qiang , Ding Ranran , Gao Qiufang , Du Kaiyue , Ma Zhen , Jin Zhi , Gao Shuhong , Qu Feng 
Published: February 2024

Abstract
Candidemia often poses a diagnostic challenge due to the lack of specific clinical features, and delayed antifungal therapy can significantly increase mortality rates, particularly in the intensive care unit (ICU). This study aims to develop a machine learning predictive model for early candidemia diagnosis in ICU patients, leveraging their clinical information and findings. We conducted this study with a cohort of 334 patients admitted to the ICU unit at Ji Ning NO.1 people’s hospital in China from Jan. 2015 to Dec. 2022. To ensure the model’s reliability, we validated this model with an external group consisting of 77 patients from other sources. The candidemia to bacteremia ratio is 1:1. We collected relevant clinical procedures and eighteen key examinations or tests features to support the recursive feature elimination (RFE) algorithm. These features included total bilirubin, age, platelet count, hemoglobin, CVC, lymphocyte, Duration of stay in ICU, neutrophils, monocyte, antibiotic therapy, PCT, CRP, WBC, immunosuppressive drugs, TPN, abdominal surgery, chemotherapy and solid cancer. To construct the candidemia diagnosis model, we employed random forest (RF) algorithm alongside other machine learning methods and conducted internal and external validation with training and testing sets allocated in a 7:3 ratio. The RF model demonstrated the highest area under the receiver operating characteristic (AUROC) with values of 0.87 and 0.83 for internal and external validation, respectively. To evaluate the importance of features in predicting candidemia, Shapley additive explanation (SHAP) values were calculated and results revealed that total bilirubin and age were the most important factors in the prediction model. This advancement in candidemia prediction holds significant promise for early intervention and improved patient outcomes in the ICU setting, where timely diagnosis is of paramount crucial.
Tumor microenvironment and immune function analysis and chemotherapy prediction of SDHB in PPGL (pheochromocytoma/ paraganglioma)
Authors: Chen Danlei , He Yi , Yang Junfeng 
Published: February 2024

Abstract
Background
SDHB mutations are risk factors for PPGL metastasis and poor prognosis. This study aimed to identify the SDHB gene signature and mechanisms in PPGL, and investigate its association with immunotherapy response.
Method
PPGL transcriptome, clinical, and single nucleotide mutation data were obtained from TCGA database. Univariate, LASSO, and multivariate Cox regression analysis was applied to construct the prognostic signature. Survival analysis, ROC curve, Cox regression analysis, and nomoplot were utilized to evaluate accuracy of the model. GO and KEGG enrichment of differentially expressed genes between risk groups were used to explore potential action mechanisms. Prognostic lncRNA co-expressed with risk signature genes were also identified. The CIBERSORT, ssGSEA, GSVA, and ESTIMATE algorithms were employed to assess the association between risk score and variations of tumor microenvironment, immune cell infiltration, immune checkpoints, and immune responses. The maftools and pRRophetic packages were enrolled to predict tumor mutation burden and drug sensitivity.
Result
A signature of SDHB genes were identified immune checkpoint and alternative splicing, which showed great value of mechanisms for PPGL. Functional enrichment implied the variation of immune pathways and metallopeptidase activity between expression groups. High- expression group exhibited higher immune score, but lower tumor purity. Finally, we screened sensitive drugs for different risk groups.
Conclusion
The novel prognostic signature of cuproptosis genes could help risk stratification, immunotherapy response prediction, and individualized treatment strategy-making for glioma patients.
Dynamical reliability of the stochastic power systems with discrete random variability
Authors: Hu Rongchun , Zeng Zheng , Lu Kang , Lu Xiang , Wang Xuefeng 
Published: February 2024

Abstract
In this paper a novel method is presented to analyze the dynamical reliability of the stochastic power systems with discrete random variability. It is inevitable for the power systems to suffer from external stochastic disturbance. At the same time, the components failure will bring abrupt changes in its substructures, which can be considered as the internal stochastic disturbance. It is demonstrated that the components failure performs random jumpy factors switching between a finite number of modes. This salient feature allows us to identify this type of dynamic behaviors as response of the hybrid power systems undergoing Markovian jumps. Utilizing a two-step approximate technique, the considered multi-DOF hybrid system can be reduced to one-dimensional averaged Itô equation of the form of system’s total energy. The approximate analytical solution of the associated back Kolmogorov (BK) equation of system’s energy is derived to predict the dynamical reliability of the original hybrid systems.
Racial differences in familiarity, interest, and use of integrative medicine among patients with breast cancer
Authors: Freeman Jincong Q. , Sheade Jori B. , Zhao Fangyuan , Olopade Olufunmilayo I. , Huo Dezheng , Nanda Rita 
Published: February 2024

Abstract
Purpose
Integrative medicine (IM) has received ASCO endorsement for managing cancer treatment-related side effects. Little is known about racial differences in familiarity, interest, and use of IM among breast cancer patients.
Methods
Breast cancer patients enrolled in the Chicago Multiethnic Epidemiologic Breast Cancer Cohort were surveyed regarding familiarity, interest, and use of IM: acupuncture, massage, meditation, music therapy, and yoga. Familiarity and interest, measured by a 5-point Likert scale, was modeled using proportional odds. Use was self-reported, modeled using binary logistic regression.
Results
Of 1,300 respondents (71.4% White and 21.9% Black), Black patients were less likely than White patients to be familiar with acupuncture (aOR 0.60, 95% CI: 0.41-0.87). While there was no differences in interest in acupuncture between Black and White patients (aOR 1.12, 95% CI: 0.76-1.65), Black patients were more interested in massage (aOR 1.86, 95% CI: 1.25-2.77), meditation (aOR 2.03, 95% CI: 1.37-3.00), music therapy (aOR 2.68, 95% CI: 1.80-3.99) and yoga (aOR 2.10, 95% CI: 1.41-3.12). Black patients were less likely than White to have used acupuncture (aOR 0.49, 95% CI: 0.29-0.84); but there were no racial differences in use of massage (aOR 0.83, 95% CI: 0.53-1.30), meditation (aOR 0.82, 95% CI: 0.47-1.43), music therapy (aOR 1.65, 95% CI: 0.82-3.32) and yoga (aOR 0.67, 95% CI: 0.37-1.20).
Conclusion
Black patients expressed more interest in IM than their White counterparts; there were no racial differences in IM use, except lower acupuncture use among Black patients. A breast program focused on equity should provide access to these services for breast cancer patients.
Target-Mediated Drug Disposition Model for Drugs with N&gt;2 Binding Sites That Bind to a Target with One Binding Site
Authors: Gibiansky Leonid , Ng Chee M , Gibiansky Ekaterina 
Published: February 2024

Abstract

The paper extended the TMDD model to drugs with more than two (N > 2) identical binding sites (N-to-one TMDD). The quasi-steady-state (N-to- one QSS), quasi-equilibrium (N-to- one QE), irreversible binding (N-to- one IB), and Michaelis-Menten (N-to-one MM) approximations of the model were derived. To illustrate properties of new equations and approximations, N = 4 case was investigated numerically. Using simulations, the N-to-one QSS approximation was compared with the full N-to-one TMDD model. As expected, and similarly to the standard TMDD for monoclonal antibodies (mAb), N-to-one QSS predictions were nearly identical to N-to-one TMDD predictions, except for times of fast changes following initiation of dosing, when equilibrium has not yet been reached. Predictions for mAbs with soluble targets (slow elimination of the complex) were simulated from the full 4-to-one TMDD model and were fitted to the 4-to-one TMDD model and to its QSS approximation. It was demonstrated that the 4-to-one QSS model provided nearly identical description of not only the observed (simulated) total drug and total target concentrations, but also unobserved concentrations of the free drug, free target, and drug-target complexes. For mAb with a membrane-bound target, the 4-to-one MM approximation adequately described the data. The 4-to-one QSS approximation converged 8 times faster than the full 4-to-one TMDD.
Free Control Based on Chaotic Systems Without Equilibrium
Authors: Zhao Boyu , Ye Xiaolin 
Published: February 2024

Abstract
Chaotic system without equilibrium is a hot topic in recent years, and its unique dynamic characteristics have caused extensive research. In this paper, different chaotic systems without equilibrium are obtained by modifying the Sprott-A system equations. The test results show the first new system is conservative. After investigating the hidden dynamical behavior of the first system, the rotation attractor phenomenon was observed by the rotation factor. Next, we make a second transformation of the Sprott-A system. By introducing trigonometric function, we can get a new system that can generate infinite coexisting grid attractors. After calculation, we find that the new system still belongs to the chaotic system without equilibrium point. It is different from chaotic system with equilibrium point, which depends on the movement of equilibrium point to produce attractor coexistence. The new system proposed in this paper has no equilibrium points, and the coexistence attractor phenomenon can be observed only by changing initial values. The chaotic system without equilibrium point which can realize the coexistence of attractors provides potential application value for some related fields, it is worthy of further study.
Association of Socio-demographic Characteristics, Clinical Profile, and Intestinal Parasite Carriage with Disease Severity among COVID-19 Cases at St. Paul Hospital Millennium Medical College, Addis Ababa, Ethiopia
Authors: Getahun Abdulhamid , Gize Addisu , Mamo Hassen 
Published: February 2024

Abstract
This study aimed to investigate the association between disease severity and demographic characteristics, clinical features, and prevalence of intestinal parasite carriage among patients with COVID-19 in Addis Ababa, Ethiopia. A cross-sectional study was conducted at St. Paul's Hospital Millennium Medical College, involving 337 COVID-19 patients. Patient data were collected during hospitalization to assess clinical outcomes, including demographic information, clinical features, and associated factors. Blood and stool samples were collected and analyzed for hematological and biochemical tests, as well as the presence of intestinal parasites. Statistical analysis was performed using the Statistical Packages for Social Sciences v. 26, employing descriptive statistics, chi-square tests, and logistic regression. A p-value of < 0.05 was considered statistically significant. Among the 337 patients with confirmed SARS-CoV-2 infection through PCR testing, 96(28.5%) were found to have co-infections with one or more intestinal parasites. Multivariate analysis revealed that patients aged ≥ 60 years [Adjusted Odds Ratio (AOR) 3.943 (95% CI: 1.792–8.673), p < 0.001], elevated creatinine levels [AOR 2.254 (95% CI: 1.041–4.881), p = 0.039], increased glutamate oxaloacetate transaminase levels [AOR 2.219 (95% CI: 1.234–3.991), p = 0.008], decreased hematocrit levels [AOR 8.839 (95% CI: 4.914–15.900), p < 0.001], and co-infection with intestinal parasites [AOR 2.419 (95% CI: 1.278–4.579), p = 0.007] were significantly associated with increased disease severity in COVID-19 patients. These findings suggest a potential link between co-infection with intestinal parasites and a more severe form of COVID-19, indicating that parasite-induced immunomodulatory responses may hinder the essential immune response against SARS-CoV-2.
Metformin-induced changes of the gut microbiota in patients with type 2 diabetes mellitus: Results from a prospective cohort study
Authors: Gao Yuting , zhao Tianyi , Lv Na , Liu Shixuan , Yuan Tao , Fu Yong , Zhao Weigang , Zhu Baoli 
Published: February 2024

Abstract
Background
The microbiota's impact on hypoglycemic agents is increasingly evident. However, the influence of metformin, a primary anti-diabetes drug, on gut microbiota remains unclear.
Research design and methods
This prospective cohort study aimed to investigate the longitudinal effects of metformin on the gut microbiota in 25 newly diagnosed, treatment-naïve diabetes patients receiving 1500 mg daily doses. Microbiota compositions were analyzed at baseline, 1, 3, and 6 months using 16S rRNA gene se-quencing.
Results
Metformin showed clinical improvements, but no significant dissimilarity in microbiome composition structure (Adonis: P = 0.077). Species richness and intestinal flora diversity declined, and α diversity significantly decreased after 3 months. The Firmicutes to Bacteroidetes ratio (ANOVA: P = 0.037) markedly decreased. Metformin treatment consistently increased Escherichia-Shigella and decreased Romboutsia, while Pseudomonas decreased at 3 months. Fuzzy c-means clustering identified three longitudinal trajectory clusters for microbial fluctuations: (i) genera temporarily changing, (ii) genera continuing to decrease (Bacteroides), and (iii) genera continuing to increase(Lachnospiraceae ND3007 group, [Eubacterium] xylanophilum group, Romboutsia, Faecalibacterium and Ruminococcaceae UCG-014). The correlation matrix revealed associations between specific fecal taxa and metformin-related clinical parameters hemoglobin A1c (HbA1c), fasting plasma glucose (FPG), Uric Acid (UA), high-density lipoproteincholesterol (HDL-C), alanine aminotransferase (ALT), C-reactive protein(CRP), triglyceride(TG) (p < 0.05). Metacyc database showed that metformin significantly altered 17 functional pathways. Amino acid metabolism pathways such as isoleucine biosynthesis predominated in the post-treatment group.
Conclusions
Metformin's role in glucose metabolism regulation may primarily involve specific alterations in certain gut microbial species rather than an overall increase in microbial species diversity. This may suggest gut microbiota targets in future studies on metabolic abnormalities caused by metformin.
MELAS with mtDNA 3243A&gt;G mutation presenting as bilateral symmetric occipital and temporal cortices lesions: a case report and literature review
Authors: Liu Qing , Wang Zhaoxia , Shi Jing , Wang Wenxia , Wen Chao , Zhu Yanping , Chen Xuan , Xing Xiaolian , Su Yangli 
Published: February 2024

Abstract
Background and Purpose: Mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) is one of the most common maternally inherited mitochondrial diseases. Stroke-like episode affecting the cortical cortex is the hallmark of MELAS, however, it rarely presents as simultaneously bilateral symmetric cortices lesions.
Methods: We reported a case of MELAS in a 46-year-old female patient with bilateral symmetric occipital and internal temporal cortices involvements on brain magnetic resonance imaging (MRI). A literature review of MELAS patients and a retrospective analysis were performed.
Results: We reported a case of MELAS in a 46-year-old female patient with cortical blindness and cognitive decline as well as status epilepticus. She had a family history of diabetes. Although she denied a history of diabetes, elevated blood glucose was noted after admission and diabetes was diagnosed. Laboratory examination revealed elevated lactate acid and creatine kinase levels in blood. Cranial computed tomography (CT) image demonstrated basal ganglia calcification, as well as subtle decreased attenuation in bilateral symmetric occipital and internal temporal cortices. Brain magnetic resonance imaging (MRI) demonstrated symmetric gyriform hyperintensity in bilateral occipital lobes and internal temporal lobes in both grey and white matter on fluid-attenuated inversion recovery (FLAIR) images with restricted diffusion on diffusion weighted images (DWI). A genetic test revealed a point mutation in the mtDNA(3243A > G) by blood examination. Literature review showed there were 231 eligible patients with MELAS were identified from 212 published papers. Symmetric cortical involvements were seen in 15(6.5%) patients on brain MRI.
Conclusions: MELAS should be considered as a potential diagnosis in the patients with bilateral symmetric stroke-like cortices lesions.
Cardiac Safety of Ribociclib Evaluated with 24-hour- Rhythm Holter Electrocardiogram
Authors: Yasin Ayse Irem , Uluganyan Mahmut , Isleyen¹ Zehra Sucuoglu , Topcu¹ Atakan , Shbair Abdallah Tm , Simsek Melih , Besiroglu Mehmet , ERSOY YELİZ EMİNE , Turk Hacı Mehmet , ŞEKER MESUT 
Published: February 2024

Abstract
Objective
We aimed to evaluate cardiac safety profile of ribociclib with 24-hour-rhythm holter ECG.
Material-Method:
42 female metastatic breast cancer patients were included in the study. Rhythm holter ECG was performed before starting treatment with ribociclib and after 3 months of the treatment initiation.
Results
The mean age of the patients was 56.36 ± 12.73. 52.4%(n = 22) of the patients were using ribociclib in combination with fulvestrant and 47.6% (n = 20) with aromatase inhibitors.None of the patients developed cardiotoxicity. When the rhythm holter results before and in third month of the treatment were compared, there was no statistically significant difference.
Conclusion
This is the first study evaluating effects of ribociclib treatment on cardiac rhythm with holter ECG. The study highlighted cardiac safety profile of ribociclib.
Enhancing Spatial-Temporal Traffic Predictor with Dynamic Road Network Adaptation
Authors: Li Minghui , Wang Wenbo , Zhang Yajing , Shen Zhou 
Published: February 2024

Abstract
This paper presents a pioneering methodology for enhancing spatial-temporal traffic prediction through the incorporation of a dynamic road network adaptation mechanism. The escalating complexity of urban traffic patterns necessitates more adaptive and accurate predictive models. Our approach addresses the limitations of static models by continuously updating the road network topology based on real-time data streams from diverse sources. Experimental evaluations, comparing our methodology with traditional machine learning, deep learning, and real-time data integration models, showcase its superior performance in terms of Mean Absolute Error (MAE), Root Mean Squared Error (RMSE), and R-squared (R2). The dynamic road network adaptation mechanism, a key innovation in our approach, allows the model to dynamically adjust to changing road conditions, thereby offering a more holistic solution to spatial-temporal traffic prediction. This research contributes significantly to the field of intelligent transportation systems, providing a foundation for creating adaptive models that can accurately predict and respond to the dynamic nature of urban traffic.
G-TERCOA: Uma década de formação e debate sobre a Educação Básica no Brasil
Authors: Costa dos Santos Maria José 
Published: February 2024

Resumo
O Grupo de estudo e pesquisa Tecendo Redes Cognitivas de Aprendizagem (G-TERCOA/CNPq), foi idealizado em 2014, pela professora Maria José Costa dos Santos, na Faculdade de Educação da Universidade Federal do Ceará (FACED/UFC). Objetiva-se apresentar esse grupo e suas discussões sobre as temáticas da educação, em especial as sobre currículo, avaliação, metodologias, teorias, tecnologias digitais, políticas públicas, formação docente, à luz da pesquisa, ensino e extensão. Neste relato de natureza qualitativa, analisa-se à luz da análise de conteúdo, os depoimentos via formulário eletrônico, de alguns de seus membros. Os depoimentos revelam que há redução na distância entre ensino, pesquisa e extensão por meio do intercâmbio escola e academia. Considera-se que as influências das atividades desenvolvidas no grupo colaboram exponencialmente para melhorias nas práticas pedagógicas, bem como para o desenvolvimento profissional dos professores das redes públicas municipais e estadual do Ceará. Assim, informa-se que semestralmente lança-se edital para seleção de novos membros que devem ter disponibilidade para participação nos encontros semanais de estudos.
Palavras-chave: G-Tercoa; Formação de professores; ensino; pesquisa; extensão.