bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eradication of Helicobacter pylori in general practice
Authors: PENSTON J. G. , MISTRY K. R. 
Published: December 2004

Objectives: To determine the current management of patients with Helicobacter pylori infection in general practice with regard to the selection of patients, testing for the presence of the organism, the choice of eradication therapy and the occurrence of symptoms during follow‐up. Design: Cross‐sectional survey involving the retrospective collection of data from patient medical records. Setting: Five general practices in the Fife region of Scotland during June and July 1995. Main outcome measures: The proportion of patients appropriately selected for eradication therapy and the success of treatment as assessed by remission of symptoms during follow‐up. Results: Of 154 patients studied, 80% received eradication therapy for peptic ulcer disease, the remainder for non‐ulcer dyspepsia or gastro‐oesophageal reflux. Fifty‐six different regimens were used, the most common combination of drugs being omeprazole plus amoxycillin. H. pylori status was known in only one‐third of patients before treatment and in only 15% after treatment. More than half of patients complained of recurrent symptoms of dyspepsia during follow‐up after eradication therapy and 47% required further treatment. In terms of the selection of patients and testing for H. pylori, the overall management using eradication therapy was acceptable in less than half of patients. In particular, the management of patients with ulcer disease associated with either ulcerogenic drugs or previous complications failed to provide the essential protection against the high risk of future haemorrhage or perforation. Conclusions: The findings of this study indicate an urgent need for improved education if therapy for the eradication of H. pylori is to be used appropriately and safely.
Diagnosis and current therapy of Wilson's disease
Authors: Ferenci P. 
Published: December 2004

SummaryWilson's disease is an autosomal recessive inherited disorder of hepatic copper metabolism resulting in liver disease and/or neuropsychiatric disease. The diagnosis of neurological disease is straightforward if the following symptoms are present: Kayser–Fleischer rings, typical neurological symptoms and low serum ceruloplasmin levels. The diagnosis is more complex in patients presenting with liver diseases. None of the commonly used parameters alone allows a diagnosis with certainty. A combination of various laboratory parameters is necessary to firmly establish the diagnosis. In the future, limited mutation analysis may play an important diagnostic role. Recently, a group of international experts has proposed a score based on a variety of tests and clinical symptoms. The validity of this score needs to be assessed prospectively. Treatment requires life‐long administration of copper chelators (d‐penicillamine, trientine). A frequently used alternative is zinc. None of these treatments has been tested by prospective randomized controlled studies. Liver transplantation is reserved for severe or treatment‐resistant cases with advanced liver disease, whilst experience with refractory neuropsychiatric disease is limited.
Anatomicohistological Characteristics of the Tubular Genital Organs of the Female Collared Peccary (Tayassu tajacu) from North‐eastern Amazon
Authors: Mayor P. , Jori F. , López‐Béjar M. 
Published: December 2004

SummaryThe present study examines anatomical and histological characteristics of tubular genital organs and its relationships with the reproductive state of 24 wild adult collared peccary (Tayassu tajacu) females. The tunica mucosa of the uterine tube presents a pseudostratified, intermittently ciliated columnar epithelium. The epithelial secretory cells of pregnant females and females in the luteal phase of the oestrous cycle became taller than the ciliated cells and showed abundant apical secretory blebs, whereas secretory cells of females in the follicular phase showed abundant mucous secretory activity (periodic acid‐Schiff positive cells). The uterus is composed of two narrow and convoluted uterine horns, separated by the velum uteri, a small uterine body and a long and muscular cervix. The endometrial lining of both uterine horns and body is a monostratified, columnar ciliated epithelium. Pregnant females and females in luteal phase showed a more developed hyperplasia of the endometrial simple tubular glands than females in the follicular phase. The cervix presents interdigitated rows of mucosal prominences that project into the lumen, structures similar to pulvini cervicali, occluding the cervical canal. In pregnant females, the endocervical canal was filled by a viscous cervical secretion. Females in follicular phase presented a thicker vaginal epithelium than pregnant females and females in luteal phase. The present study suggests that the collared peccary female showed different histological features of the uterine tubes, uterus and vagina in accordance with the reproductive state of the females.
Morphological Aspects of In Vivo Cleavage in Myocastor coypus (coypu)
Authors: Felipe A. E. , Teruel M. T. , Cabodevila J. A. , Callejas S. S. 
Published: December 2004

SummaryThe objective of the present work was to characterize the in vivo cleavage stage of Myocastor coypus embryos. For this purpose a colpocytological follow‐up and controlled mating of 18 females were performed. Specimens from the beginning of the first cleavage to the acquisition of a morula appearance were considered to be in cleavage stage. Embryos in cleavage were collected between days 3 and 6 post‐coitus. Of the collected embryos, 80% presented an even number of blastomeres and the remaining 20% an odd number. Embryos from 3 to 7 cells were blastomere associations in a spherical disposition within the zona pellucida. Blastomeres were spherical or ovoid, presenting slight flattening in areas contacting with other blastomeres. Embryos of 8 and 9 cells were as a group of blastomeres slightly elongated, surrounded by a spherical zona pellucida. The percentage of peri‐vitelline space occupied by the embryonic mass ranged from 74.1 to 95.8% for all the substages. The cleavage pattern, developed in the oviduct, was of a rotational holoblastic type and asynchronic.
Distribution of the predatory mite Amblyseius andersoni Chant (Acari: Phytoseiidae) on different apple cultivars
Authors: Duso C. , Pasini M. 
Published: December 2004

AbstractThe effect of apple cultivar on the distribution of Amblyseius andersoni was studied in an experimental orchard where tetranychids were virtually absent and eriophyids reached low to moderate populations. Seven apple scab‐resistant cultivars, showing some differences in their leaf morphology, were compared. Each cultivar was grafted onto three different rootstocks inducing increasing vigor. The intraplant distribution of A. andersoni was also studied by monitoring predator densities on basal and apical leaves. In the first year, the colonization of different cultivars by A. andersoni showed definite patterns, sometimes associated with the abundance of the eriophyid Aculus schlechtendali. In the second experimental season, eriophyids reached negligible levels and the effect of cultivar on A. andersoni abundance was less clear. Florina and N.Y. 18 491 cultivars were colonized in both years by relatively high phytoseiid numbers, while TSR 29T219 and Priscilla showed low populations in at least one season. The results observed on the remaining cultivars were intermediate or indefinite. The role of leaf pubescence on phytoseiid colonization is discussed.
Conjugation of δ‐endotoxin from Bacillus thuringiensis with abamectin of Streptomyces avermitilis as a new type of biocide, GCSC‐BtA, for control of agricultural insect pests
Authors: Liu B. , Sengonca C. 
Published: December 2004

AbstractConjugation of δ‐endotoxin from Bacillus thuringiensis with abamectin, a toxin of Streptomyces avermitilis, was carried out to form a new type of biocide, GCSC‐BtA based on “Germany‐China Scientific Cooperation” research, for the control of agricultural insect pests. The strategy for biochemical linkage was designed by conjugating an amino group in B.t. protoxin with a carboxyl group in carboxylated abamectin under the treatment of conjugator EDC [1‐Ethyl‐3‐(3‐dimethylaminopropyl carbodiimide Hydrochloride)]. The formation of B.t. protoxin was processed by solubilizing B.t. crystal in 25 mM dithiothreitol (DTT) at 37 °C for 2 h. The carboxylated abamectin was formed by carboxylating the NaH‐activated abamectin with 10 mg/ml butyric anhydride at 111 °C in a water‐circumfluent condensation device for 2 h. The conjugating reaction, consisting of 5 mg/ml B.t. protoxin, 10 mg/ml carboxylated abamectin and 19.17 mg/ml EDC, was successfully conducted at room temperature for 24 h. Significant differences were found between pure abamectin, carboxylated abamectin and the conjugated BtA by means of UV‐photo absorptions recorded at wavelengths 354, 438, 518, 600 nm (P < 0.01). LT50 of the conjugated GCSC‐BtA to the 3rd instar larvae of Plutella xylostella (L.) (Lep., Plutellidae) was 35.27 μg a.i./ml, about 62 % and 76 % of that caused by the B.t. protoxin and the caxboxylated abamectin, respectively. The conjugated GCSC‐BtA caused 87.14 % mortalities in larvae of P. xylostella, 93.75 % in adult Myzus persicae (Sulzer) (Hom., Aphididae) and 89.33 % in adult Phyllotreta vittata Fabricius (Col., Chrysomelidae) as compared to 48.33 % by the B.t. crystal only in P. xylostella. The symptoms caused by conjugated GCSC‐BtA in the 3rd instar of P. xylostella were black color in the head part and white‐yellow in the abdomen of dead larvae, which differed from the black color or the white‐yellow all along the body caused by either the B.t. crystal or the abamectin, respectively. It was concluded that the conjugated GCSC‐BtA biocide had a broader host spectrum and a faster killing speed than either the B.t. crystal or abamectin alone for the control of agricultural pests.
Contributions to the knowledge of conifer aphid fauna in Turkey and their zoogeographical distribution
Authors: Toper Kaygi̇n A. , Çanakçi̇oğlu H. 
Published: December 2004

AbstractThe Aphidoidea (Homoptera) has 29 species which attack coniferous trees in Turkey. The family Aphididae has 25 (Aphidinae, 1; Lachninae, 22; Mindarinae, 2) species and the Adelgidae, 4. The tribe Cinarini in Lachninae has alone 22 species most of which are important. Especially Cinara cedri Mimeur on Cedrus libani and C. pilicornis (Hartig) on Picea spp. are harmful species. Schizolachnus pineti (Fabricius) is also very important. An Eastern Palearctic species in the tribe Macrosiphini in Aphidinae, Elatobium momii (Shinji), is numerous on Abies bornmülleriana in Bursa‐Uludağ forests. Pineus orientalis (Dreyfus) in the family of Adelgidae (Chermesidae) is the most destructive in our Picea orientalis forests in Artvin area (Eastern Black‐Sea Section). 21 species out of 29 aphids on coniferous trees are of European origin; only 4 are Mediterranean, 2 Caucasus and Turkistan, one Eastern Palearctic and one of Cosmopolitan origin.
Site condition and predation influence a bark beetle's success: a spatially realistic approach
Authors: Gilbert Marius , Grégoire Jean‐Claude 
Published: December 2004

Abstract1 Spatial pattern in abundance ofDendroctonus micanswas studied in a 600‐ha spruce stand in the Massif Central (Lozère, France). The proportion of trees attacked was measured in 38 plots and these data were used to estimate spatial pattern of attack density in the stand and to identify a transect of decreasing attack density (80% to 30%) over less than 1000 m.2 Spatial variation in attack density was analysed in relation to (i) data on site and stand characteristics (altitude, slope, tree density, tree average height, yield class and average age) collected from 63 points in the stand and (ii) the releases of the predatorRhizophagus grandis(localization and number of beetles released).3 The proportion of attacked trees was analysed using geostatistics and showed a strong spatial structure reflecting the spatial scale of interaction ofD. micanswith its environment. The spatial structure was modelled in order to estimate the spatial distribution of attack density at unsampled locations.4 A linear model relating interpolated attack density to the number of predators released 6–10 years before the survey in a 300‐m radius and to the average slope over a 250‐m radius explained 67% of the observed variability. Spatial autocorrelation was taken into account in a spatial regression model.
Phylogenetic analysis of a group of palaeonemerteans (Nemertea) including two new species from Queensland and the Great Barrier Reef, Australia
Authors: Sundberg Per , Gibson Ray , Olsson Urban 
Published: December 2004

Sundberg, P., Gibson, R. & Olsson, U. (2003). Phylogenetic analysis of a group of palaeonemerteans (Nemertea) including two new species from Queensland and the Great Barrier Reef, Australia. —Zoologica Scripta, 32, 279–296.Based on 18S rDNA nucleotide sequences and morphological characters, we reconstruct the phylogeny for a group of palaeonemerteans estimated to be monophyletic. Two new palaeonemertean species from Queensland and the Great Barrier Reef, Australia are included in the phylogenetic analysis. The results confirm that one of the species, Cephalothrix queenslandica sp. n., is part of the Cephalothrix–Cephalotrichella–Procephalothrix group. These genera are redefined phylogenetically under the name Cephalothrix based on the cladistic analysis. The other species, Balionemertes australiensis gen. et sp. n., is placed in a new genus which forms a sister taxon to Cephalothrix. The morphology of both new species is described in detail.
A phylogenetic analysis of Tubificinae and Limnodriloidinae (Annelida, Clitellata, Tubificidae) using sperm and somatic characters
Authors: Marotta Roberto , Ferraguti Marco , Erséus Christer 
Published: December 2004

Marotta, R., Ferraguti, M. & Erséus, C. (2003). A phylogenetic analysis of Tubificinae and Limnodriloidinae (Annelida, Clitellata, Tubificidae) using sperm and somatic characters. —Zoologica Scripta, 32, 255–278.The spermatozoa and the sperm aggregates of 13 species belonging to four genera (Smithsonidrilus, Limnodriloides, Thalassodrilides, Doliodrilus) in the tubificid subfamily Limnodriloidinae (Annelida, Oligochaeta) were studied and compared with the spermatozoal patterns already described in the subfamily Tubificinae. Two characters considered exclusive for the Tubificinae were found in the more spermatologically variable Limnodriloidinae: the production of two kinds of spermatozoa, eusperm and parasperm, and the presence, in the spermathecae, of sperm aggregates formed by a combination of the two sperm types. A parsimony analysis was performed on the spermatozoal data of the species examined and compared with that based on the somatic characters of the same species: a critical revision of the already codified eusperm characters was carried out and the ultrastructure of parasperm was used as a new subset of spermatozoal characters. In a total evidence approach, a further parsimony analysis was run using a matrix combining both sets of characters. This analysis suggested that the double sperm line and the sperm aggregates composed of both eusperm and parasperm may well be homologous in tubificines and limnodriloidines. It thus supported the previous notion that Tubificinae and Limnodriloidinae are closely related and indicated that these subfamilies may be sister taxa.
REPORT on the Fifth International Workshop on Chromosome 9 held at Eynsham, Oxfordshire, UK, September 4–6, 1996
Authors: POVEY S. , ATTWOOD J. , CHADWICK B. , FREZAL J. , HAINES J. L. , KNOWLES M. , KWIATKOWSKI D. J. , OLOPADE O. I. , SLAUGENHAUPT S. , SPURR N. K. , SMITH M. , STEEL K. , WHITE J. A. , PERICAK‐VANCE M. A. 
Published: December 2004

The Fifth International workshop on chromosome 9 comprised a gathering of 36 scientists from seven countries and included a fairly even distribution of interests along chromosome 9 as well as a strong input from more global activities and from comparative mapping. At least eight groups had participated in the goal set at the previous workshop which was to improve the fine genetic mapping in different regions of chromosome 9 by meiotic breakpoint mapping in allocated regions and this has resulted in some greatly improved order information. Excellent computing facilities were available and all contributed maps were entered not only into SIGMA (and thence submitted to GDB) but also into a dedicated version of ACEDB which can be accessed on the Web in the form of one of 28 slices into which the chromosome has been arbitrarily divided. It was generally agreed that the amount of data is now overwhelming and that the integration and validation of all data is not only unrealistic in a short meeting but probably impossible until the whole chromosome has been sequenced and fully annotated. Sequence‐ready contigs presented at the meeting totalled about 3 MB which is about one fiftieth of the estimated length. The single biggest barrier to integration of maps is the problem of non‐standard nomenclature of loci. In the past 2 workshops efforts have been made to compare traditional ‘consensus’ maps made by human insight (still probably best for small specific regions) with those generated with some computer assistance (such as SIGMA) and those generated objectively by defined computer algorithms such as ldb. Since no single form of map or representation is entirely satisfactory for all purposes the maps reproduced in the published version of the report are confined to one of the genetic maps, in which Genethon and older markers have been incorporated, a Sigma map of the genes as symbols together with a listing of known ‘disease’ genes on chromosome 9, and a revised assessment of the mouse map together with a list of mouse loci predicted to be on human chromosome 9. One of the 28 ACEDB slices is also shown to illustrate strengths and weaknesses of this approach. Workshop files include not only all maps available at the time but also details of loci and details of the meiotic breakpoints in the CEPH families (http://www.gene.ucl.ac.uk/scw9db.shtml).This report and other information on chromosome 9 can be found on the chromosome 9 homepage at the URL: http://www.gene.ucl.ac.uk/chr9/
The sib‐pair problem. . I. Affected pairs with parents. Constant penetrance models
Authors: EDWARDS J. H. 
Published: December 2004

Affected sib pairs with typed but unaffected parents, conveniently termed foursomes, have become a major source of information on genetic susceptibility to common disease. So far most methods of analysis have been based on extensions of the single locus analyses developed, and successfully applied, to the mendelian disorders. However, unifactorial methods are not suited to multifactorial disorders. The power of methods of detecting linkage in the presence of more than one locus with one or more susceptibility alleles is considered.The relevance of familial clustering to predicting the presence of loci with susceptibility or resistance alleles sufficiently frequent and effective to have an appreciable influence on population incidence is discussed. The mathematical problem of clustering due to numerous alleles of small effect was resolved by Pearson in 1901 in relation to claims that the mendelian model of an allele at a single locus determining a distinct phenotype was necessary to explain the familial concentrations that had been observed in several species. The apparent inconsistency between the mendelian and polygenic models was resolved by Fisher's demonstration in 1918 that there was no essential difference between these two extreme forms of phenotypic determination. Although constant penetrance models are unrealistic, and no longer necessary since Pearson's analysis, the assumption is implicit in most recent analyses and has the advantage of simplicity in providing a lower limit on the sample sizes necessary.
Neurotransmitter release in an arterial preparation and the action of α‐adrenoceptor antagonists
Authors: Kalsner S. , Abdali S. A. 
Published: December 2004

Summary 1 This study examined the potentiating effects of competitive antagonists of the adrenergic alpha2 receptors and of phenoxybenzamine (POB) an irreversible antagonist, on the stimulation‐induced efflux of [3H]‐noradrenaline in arterial tissue of rabbit. This was done to determine if the lack of concordance of efflux potentiation by antagonists with the expectations of presynaptic negative feedback theory can be attributed to increasingly successful competition from rising perineuronal transmitter concentrations, when stimulation parameters are increased, in the presence of a fixed concentration of competitive antagonist. 2 Tissues were stimulated with a fixed pulse number and frequency, to rule out confounding factors, and major alterations in the concentration of released transmitter were achieved through variations in the pulse duration. 3 Rauwolscine potentiated transmitter release less at the longest, rather than at the shortest pulse duration, and showed a potentiation of release that was indifferent to the quantities of released transmitter. This was also seen with POB although it binds covalently to the presynaptic receptor. 4 Noradrenaline inhibited stimulation‐induced transmitter release confirming the presence of presynaptic alpha inhibitory sites. 5 Yohimbine potentiated transmitter release the same as did rauwolscine and POB, and protected the relevant sites against POB potentiation, confirming site identity. The combination of POB and rauwolscine had no greater effect than did either alone certifying that they acted similarly and that maximally effective concentrations of each were used. 6 Consequently, noradrenaline breakthrough of presynaptic receptor blockade does not explain the non‐conforming observations made with competitive antagonists in tests of presynaptic theory.