bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influence of Race, Socioeconomic Status, and Physical Activity on the Mental Health Benefits of Sport Participation During COVID-19
Authors: Biese Kevin M. , McGuine Timothy A. , Haraldsdottir Kristin , Reardon Claudia , Watson Andrew M. 
Published: January 2024

Background: The purpose of this study was to compare the benefits of sport participation with no sport participation during the COVID-19 pandemic and determine the moderating effects of race and ethnicity, socioeconomic status (SES), and physical activity (PA) on mental health in adolescent athletes. Hypothesis: Sport participation would be associated with greater improvements in mental health for athletes from racial and ethnic minority and lower SES groups compared with White and high SES groups. PA would mediate <30% of the mental health benefits of sport participation. Study Design: Cross-sectional study. Level of Evidence: Level 4. Methods: In May 2021, adolescent athletes completed an online survey (demographics, sport participation, measures of anxiety and depression, PA). Participants were classified as those who played sports (PLY) and those who did not (DNP). Mental health symptoms for the PLY and DNP groups were compared via analysis of variance models that controlled for demographic variables. Moderating analyses assessed the interaction of sport participation status by (1) race and ethnicity (White/non-White) and (2) SES (high/low) with mental health. Mediation analyses assessed the degree that PA scores explained the differences in anxiety and depression between the 2 groups. Results: Participants included 4874 adolescent athletes (52% female; age, 16.1 ± 1.3 years; PLY, 91%). Athletes who returned to sport from racial minority groups and low SES had greater decreases in anxiety (race and ethnicity: interaction estimate (b) = -1.18 ± 0.6, P = 0.04; SES, b = -1.23 ± 0.5, P = 0.02), and depression (race and ethnicity: b = -1.19 ± 0.6, P = 0.05; SES, b = -1.21 ± 0.6, P = 0.03) compared with White and high SES athletes, respectively. PA explained 24% of anxiety ( P < 0.01) and 20% of depression scores ( P < 0.01). Conclusion: Athletes who identify as a racial or ethnic minority and from areas of lower household income experienced disproportionately greater negative mental health impacts from sport restrictions during COVID-19. Clinical Relevance: Improving access to sports in traditionally underserved areas may have significant mental health impact for adolescents.
Frontal Sinus Fractures and Traumatic Brain Injury: Predictors of Mortality in Surgical Management
Authors: Trejo Eduardo , García Patricio , Ortega Diego A. , González Karla C. , Botello Edgar , Hernández Gamaliel , Morales Jesús A. , Martínez Ángel R. 
Published: January 2024

Study Design This is anobservational, retrospective, analytical study. Objective The aim was todetermine a statistical basis for a future line of research based on the epidemiology of a center located in a developing country, as well as defining indirect mortality predictors. Methods Clinical files were reviewed based on diagnosis of Traumatic Brain Injury (TBI), according to the International Classification of Diseases 10th Revision (ICD-10). Sociodemographic variables as well as treatment modality of the condition during hospitalization was recorded. The patient sample was divided into two groups. Student’s T-test was performed in variables with normal distribution and Chi-square test in independent random variables with standard normal distribution. For correlations, Pearson’s correlation coefficient was used, taking the P-value <.05 as statistically significant. Results A total of 150 participants were included in this study, from which 125 were male (83.3%). The average age was 28.58 ± 16.55 years. The median hospitalization time was 9 days. Forty-five patients (30%) were treated conservatively. Fifteen patients died during hospitalization. The factors considered as predictors of mortality in the general population corresponded to Motor Vehicle Accident, Frontonasal Duct Obstruction, Neuroinfection, Glasgow Coma Scale (GCS) at admission, as well as GCS at discharge. In the patients who underwent surgery, predictors of mortality corresponded to Motor Vehicle Accident, Bilateral Frontal Craniotomy, Surgical Bleeding >475 cc, Neuroinfection, as well as GCS at admission and discharge. Conclusions The creation of adequate diagnostic and therapeutic algorithms in traumatic brain injury management is needed, especially in developing countries. More specific studies are needed, particularly analytical and multicentric studies, which may allow the development of these algorithms.
Effects of Experiential Learning Program of Community Health Workers on Health Literacy and Preventive Behaviors of Emerging Infectious Diseases and Re-Emerging Infectious Diseases of Respiratory System
Authors: Wongcharoen Neeranuch , Srirattayawong Thapkwan , Panta Pitakpong , Ongkulna Kiattisak , Teachasub Jutapong , Somboon Tanawat , Weiangkham Dao 
Published: January 2024

Introduction/Objective: Community health workers (CHWs) can be extremely important in many facets of healthcare, especially for marginalized communities. Health Literacy and Preventive Behaviors of Emerging Infectious Diseases and Re-emerging Infectious Diseases of the Respiratory System were important for CHWs in Preventing and Control Diseases. Methods: This quasi-experimental research; a 2 pretest-post group design, aimed to investigate the result of the experiential learning program of the CHWs toward health literacy and preventive behaviors of the infection of emerging and re-emerging diseases of the respiratory system. The samples are 66 CHWs working in Ngao District, Lampang Province which were divided into 2 groups; the experimental and control groups, 33 subjects for each group. The tools used in this research consisted of an experiential learning program for 6 weeks and a questionnaire concerning health literacy and preventive behaviors of emerging and re-emerging diseases in the respiratory system. Results: The experimental group had the mean score of health literacy regarding the prevention of emerging and re-emerging diseases of the respiratory system at the highest level ([Formula: see text] = 4.52, SD = 0.16) which was higher than before receiving the program which was a high level ([Formula: see text] = 3.61, SD = 0.32) at the significant level of P < .001 while the preventive behavior concerning the emerging and re-emerging diseases in the respiratory system was at the highest level ([Formula: see text] = 4.63, SD = 0.41)and higher than the control group which was at a high level ([Formula: see text] = 4.36, SD = 0.50) at the significant level of P < .001. Conclusions: The research results could be a guideline for developing the CHW’s potential effectively and proficiently.
Technique and Outcomes of Posterior Cruciate Ligament Repair With Augmentation
Authors: Loeb Alexander E. , Ithurburn Matthew P. , Kidwell-Chandler Ariel , Atkinson Andrew , Cain E. Lyle 
Published: January 2024

Background: Posterior cruciate ligament (PCL) injuries to the knee are uncommon, and ideal surgical management of these injuries is unclear. Current surgical techniques include PCL reconstruction with remnant debridement, remnant-preserving techniques, and primary PCL repair. Augmentation of PCL repairs and reconstructions has been proposed to protect repairs or grafts in the postoperative period. Purpose: To describe PCL repair with the hamstring autograft augmentation technique and examine our preliminary midterm outcomes from a sequential cohort of patients. Study Design: Case series; Level of evidence, 4. Methods: The authors identified patients at their institution who underwent remnant-preserving primary PCL repair with hamstring autograft augmentation for both isolated tears and tears associated with multiligament knee injury (MLKI). Patient-reported outcomes were evaluated at a minimum 2-year follow-up using the International Knee Documentation Committee (IKDC) subjective knee form, the 12-item Short Form Survey, and a custom return-to-play questionnaire. Patient-reported outcomes data were summarized, and the predictors of outcomes from the descriptive data and clinical measures were further examined. Results: A total of 23 patients with a mean follow-up of 5.3 years met the inclusion criteria. Of these patients, 87% were associated with MLKI. The mean IKDC score was 87.7. Approximately 83% of patients were able to successfully return to their sport or occupation. Among 19 athletes, only 2 reported being unable to return to their preinjury level of sport because of limitations from their PCL surgery. Patient-reported outcome scores and return to sport or occupation did not have a statistically significant association with age, sex, body mass index, time from injury to surgery, or follow-up time. Conclusion: Outcomes of our cohort with remnant-preserving primary PCL repairs with hamstring autograft augmentation demonstrated comparable clinical outcomes to previously published PCL data. The advantages of remnant preservation, primary repair, and augmentation with an independent hamstring autograft reconstruction are combined within this technique.
Digital denizenship: Hindu nationalist architectures of digital closings and unbelonging in India
Authors: Oza Ekta , Williams Philippa , Kamra Lipika 
Published: January 2024

This paper examines the sociodigital experiences of political and religious minorities in contemporary India to understand matters of voice and power, as well as feelings of belonging, identity and citizenship. It builds out from research conducted in New Delhi between February and June 2019 during and after the Indian national elections and focuses on WhatsApp as an everyday space where Hindu nationalism is (re)produced and articulated through memes, forwarded messages, videos and political talk. In the shadow of right-wing nationalisms, it examines how civic and political relationships are being transformed. Drawing on experiences and narratives of political and religious minorities we contend that the ‘digital denizen’ represents a new digital-political subject, one who is increasingly outcaste within a global conjuncture of exclusionary politics, technological affordances and local histories of power and coercion. Digital denizenship represents the regression of citizenship which connects ways of acting and articulating within intimate and digital storytelling infrastructures shaped by the articulation and defence of ‘truth’. We show how being a digital denizen means recognising, anticipating and navigating the oppressive expressive power of exclusionary sociodigital practices in order to quietly resist difference, disorientation, danger and unbelonging in everyday life.
α-Ketoglutarate improves cardiac insufficiency through NAD+-SIRT1 signaling-mediated mitophagy and ferroptosis in pressure overload-induced mice
Authors: Yu Hao , Gan Daojing , Luo Zhen , Yang Qilin , An Dongqi , Zhang Hao , Hu Yingchun , Ma Zhuang , Zeng Qingchun , Xu Dingli , Ren Hao 
Published: January 2024

Abstract
Background
In heart failure (HF), mitochondrial dysfunction and metabolic remodeling lead to a reduction in energy productivity and aggravate cardiomyocyte injury. Supplementation with α-ketoglutarate (AKG) alleviated myocardial hypertrophy and fibrosis in mice with HF and improved cardiac insufficiency. However, the myocardial protective mechanism of AKG remains unclear. We verified the hypothesis that AKG improves mitochondrial function by upregulating NAD+ levels and activating silent information regulator 2 homolog 1 (SIRT1) in cardiomyocytes.

Methods
In vivo, 2% AKG was added to the drinking water of mice undergoing transverse aortic constriction (TAC) surgery. Echocardiography and biopsy were performed to evaluate cardiac function and pathological changes. Myocardial metabolomics was analyzed by liquid chromatography‒mass spectrometry (LC‒MS/MS) at 8 weeks after surgery. In vitro, the expression of SIRT1 or PINK1 proteins was inhibited by selective inhibitors and siRNA in cardiomyocytes stimulated with angiotensin II (AngII) and AKG. NAD+ levels were detected using an NAD test kit. Mitophagy and ferroptosis levels were evaluated by Western blotting, qPCR, JC-1 staining and lipid peroxidation analysis.

Results
AKG supplementation after TAC surgery could alleviate myocardial hypertrophy and fibrosis and improve cardiac function in mice. Metabolites of the malate-aspartate shuttle (MAS) were increased, but the TCA cycle and fatty acid metabolism pathway could be inhibited in the myocardium of TAC mice after AKG supplementation. Decreased NAD+ levels and SIRT1 protein expression were observed in heart of mice and AngII-treated cardiomyocytes. After AKG treatment, these changes were reversed, and increased mitophagy, inhibited ferroptosis, and alleviated damage in cardiomyocytes were observed. When the expression of SIRT1 was inhibited by a selective inhibitor and siRNA, the protective effect of AKG was suppressed.

Conclusion
Supplementation with AKG can improve myocardial hypertrophy, fibrosis and chronic cardiac insufficiency caused by pressure overload. By increasing the level of NAD+, the SIRT-PINK1 and SIRT1-GPX4 signaling pathways are activated to promote mitophagy and inhibit ferroptosis in cardiomyocytes, which ultimately alleviates cardiomyocyte damage.
Mapping three-dimensional intratumor proteomic heterogeneity in uterine serous carcinoma by multiregion microsampling
Authors: Hunt Allison L. , Bateman Nicholas W. , Barakat Waleed , Makohon-Moore Sasha C. , Abulez Tamara , Driscoll Jordan A. , Schaaf Joshua P. , Hood Brian L. , Conrads Kelly A. , Zhou Ming , Calvert Valerie , Pierobon Mariaelena , Loffredo Jeremy , Wilson Katlin N. , Litzi Tracy J. , Teng Pang-Ning , Oliver Julie , Mitchell Dave , Gist Glenn , Rojas Christine , Blanton Brian , Darcy Kathleen M. , Rao Uma N. M. , Petricoin Emanuel F. , Phippen Neil T. , Maxwell G. Larry , Conrads Thomas P. 
Published: January 2024

Abstract
Background
Although uterine serous carcinoma (USC) represents a small proportion of all uterine cancer cases, patients with this aggressive subtype typically have high rates of chemotherapy resistance and disease recurrence that collectively result in a disproportionately high death rate. The goal of this study was to provide a deeper view of the tumor microenvironment of this poorly characterized uterine cancer variant through multi-region microsampling and quantitative proteomics.

Methods
Tumor epithelium, tumor-involved stroma, and whole “bulk” tissue were harvested by laser microdissection (LMD) from spatially resolved levels from nine USC patient tumor specimens and underwent proteomic analysis by mass spectrometry and reverse phase protein arrays, as well as transcriptomic analysis by RNA-sequencing for one patient’s tumor.

Results
LMD enriched cell subpopulations demonstrated varying degrees of relatedness, indicating substantial intratumor heterogeneity emphasizing the necessity for enrichment of cellular subpopulations prior to molecular analysis. Known prognostic biomarkers were quantified with stable levels in both LMD enriched tumor and stroma, which were shown to be highly variable in bulk tissue. These USC data were further used in a comparative analysis with a data generated from another serous gynecologic malignancy, high grade serous ovarian carcinoma, and have been added to our publicly available data analysis tool, the Heterogeneity Analysis Portal (https://lmdomics.org/).

Conclusions
Here we identified extensive three-dimensional heterogeneity within the USC tumor microenvironment, with disease-relevant biomarkers present in both the tumor and the stroma. These data underscore the critical need for upfront enrichment of cellular subpopulations from tissue specimens for spatial proteogenomic analysis.
A Mitochondrial Localized Chaperone Regulator OsBAG6 Functions in Saline-Alkaline Stress Tolerance in Rice
Authors: Wang Jie , Ao Min , Ma Ao , Yu Jinlei , Guo Peng , Huang Shuangzhan , Peng Xiaoyuan , Yun Dae-Jin , Xu Zheng-Yi 
Published: January 2024

AbstractB-cell lymphoma 2 (Bcl-2)-associated athanogene (BAG) family genes play prominent roles in regulating plant growth, development, and stress response. Although the molecular mechanism underlying BAG’s response to abiotic stress has been studied in Arabidopsis, the function of OsBAG underlying saline-alkaline stress tolerance in rice remains unclear. In this study, OsBAG6, a chaperone regulator localized to mitochondria, was identified as a novel negative regulator of saline-alkaline stress tolerance in rice. The expression level of OsBAG6 was induced by high concentration of salt, high pH, heat and abscisic acid treatments. Overexpression of OsBAG6 in rice resulted in significantly reduced plant heights, grain size, grain weight, as well as higher sensitivity to saline-alkaline stress. By contrast, the osbag6 loss-of-function mutants exhibited decreased sensitivity to saline-alkaline stress. The transcriptomic analysis uncovered differentially expressed genes related to the function of “response to oxidative stress”, “defense response”, and “secondary metabolite biosynthetic process” in the shoots and roots of OsBAG6-overexpressing transgenic lines. Furthermore, cytoplasmic levels of Ca2+ increase rapidly in plants exposed to saline-alkaline stress. OsBAG6 bound to calcium sensor OsCaM1-1 under normal conditions, which was identified by comparative interactomics, but not in the presence of elevated Ca2+. Released OsCaM1-1 saturated with Ca2+ is then able to regulate downstream stress-responsive genes as part of the response to saline-alkaline stress. OsBAG6 also interacted with energy biosynthesis and metabolic pathway proteins that are involved in plant growth and saline-alkaline stress response mechanisms. This study reveals a novel function for mitochondrial localized OsBAG6 proteins in the saline-alkaline stress response alongside OsCaM1-1.
Exposure to heavy metals and neurocognitive function in adults: a systematic review
Authors: Althomali Raed H. , Abbood Manal A. , Saleh Ebraheem Abdu Musad , Djuraeva Laylo , Abdullaeva Barno Sayfutdinovna , Habash Ruqayah Taher , Alhassan Muataz S. , Alawady Ahmed Hussien Radie , Alsaalamy Ali Hashiem , Najafi Moslem Lari 
Published: January 2024

AbstractExposure of individuals to heavy metals (HMs) is a growing concern with far-reaching implications for human health. HMs encompass a diverse range of elements that, when present in excess or in particular chemical forms, have the potential to elicit adverse effects on the central nervous system and cognitive function. This systematic review aims to comprehensively investigate the relationship between exposure to HMs and neurocognitive function in adults. The methodological framework for this review adheres rigorously to the Meta-analyses Of Observational Studies in Epidemiology (MOOSE) guidelines. A meticulous and extensive search strategy was executed within PubMed and Web of Science, specifically targeting articles published in the English language until the cutoff date of December 5, 2023. The evaluation of the retrieved studies was systematically conducted by employing the assessment approach outlined by (Gascon et al. in Environ Int 86 60 67, 2016). The initial search yielded a substantial pool of 1573 articles, culminating in a refined selection comprising eight pertinent studies, collectively enrolling a participant cohort totalling n = 1,828,126. Notably, the studies under review predominantly manifested a cross-sectional or cohort design and were geographically situated within the continents of North America and Asia. Furthermore, it is imperative to underscore that a predominant and recurring observation emanating from the majority of the scrutinized investigations underscores a significant correlation between exposure to cadmium (Cd) and mercury (Hg) and deleterious neurocognitive outcomes in the adult population. In summary, our systematic review postulates that exposure to HMs through various routes of exposure harbors the potential for adverse effects on adult neurocognitive function; however, it is incumbent upon future research endeavors to validate and corroborate these findings through further empirical exploration.
Genome characteristics and the ODV proteome of a second distinct alphabaculovirus from Spodoptera litura
Authors: Gao Weisong , Liu Xingjian , Gao Xintao , Wu Tong , Wei Shuang , Zhang Zhifang , Zhang Huan , Li Yinü 
Published: January 2024

Abstract
Background
Spodoptera litura is a harmful pest that feeds on more than 80 species of plants, and can be infected and killed by Spodoptera litura nucleopolyhedrovirus (SpltNPV). SpltNPV-C3 is a type C SpltNPV clone, that was observed and collected in Japan. Compared with type A or type B SpltNPVs, SpltNPV-C3 can cause the rapid mortality of S. litura larvae.

Methods
In this study, occlusion bodies (OBs) and occlusion-derived viruses (ODVs) of SpltNPV-C3 were purified, and OBs were observed by scanning electron microscopy (SEM). ODVs were observed under a transmission electron microscope (TEM).

Results
Both OBs and ODVs exhibit morphological characteristics typical of nucleopolyhedroviruses (NPVs).The genome of SpltNPV-C3 was sequenced and analyzed; the total length was 148,634 bp (GenBank accession 780,426,which was submitted as SpltNPV-II), with a G + C content of 45%. A total of 149 predicted ORFs were found. A phylogenetic tree of 90 baculoviruses was constructed based on core baculovirus genes. LC‒MS/MS was used to analyze the proteins of SpltNPV-C3; 34 proteins were found in the purified ODVs, 15 of which were core proteins. The structure of the complexes formed by per os infectivity factors 1, 2, 3 and 4 (PIF-1, PIF-2, PIF-3 and PIF-4) was predicted with the help of the AlphaFold multimer tool and predicted conserved sequences in PIF-3. SpltNPV-C3 is a valuable species because of its virulence, and the analysis of its genome and proteins in this research will be beneficial for pest control efforts.
Ultrasonic optic nerve sheath diameter can be used as a diagnostic measure after accidental dural puncture during cesarean section: a case report
Authors: Wang Pei , Zhou Xia , Sheng Fang , Wang Xiaolong , Shi Caifeng , Feng Wei 
Published: January 2024

AbstractBackgroundParturients are prone to postdural puncture headache (PDPH) after epidural puncture. Cerebral venous sinus thrombosis (CVST) is a fatal complication of PDPH. The main symptom of both is headache, however, the mechanism is not similar. For persistent PDPH, early differential diagnosis from CVST is essential. Optic nerve sheath diameter (ONSD) measurements can be used to identify changes in intracranial pressure as an auxiliary tool to distinguish the cause of headache.Case presentationThe dura of a 32-year-old woman undergoing cesarean section was accidentally penetrated while administering epidural anesthesia, and the patient developed PDPH the subsequent day. The patient refused epidural blood patch (EBP) treatment and was discharged after conservative treatment. Fourteen days post-discharge, she was readmitted for a seizure. Magnetic resonance imaging (MRI) and Magnetic resonance angiography (MRA) indicated low cranial pressure syndrome and superior sagittal sinus thrombosis with acute infarction. The next morning, the EBP was performed with 15 ml autologous blood. Subsequently, the headache symptoms decreased during the day and worsened at night. ONSD measurement suggested dilation of the optic nerve sheath, and subsequently, the patient showed intracranial hypertension with papilledema. After dehydration and anticoagulant treatment, the patient’s symptoms were relieved and she was discharged from the hospital 49 days later.ConclusionsHeadache is the main symptom of PDPH and cerebral venous thrombosis, which are difficult to distinguish. ONSD measurement may help to estimate the intracranial pressure, and early measurement may be helpful for women with PDPH to avoid serious complications, such as CVST.