bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gaya Kepemimpinan Transformasional dan Kesejahteraan Psikologis Karyawan di PT Primayudha Mandirijaya Kabupaten Boyolali Jawa Tengah
Authors: Abadi Andhika Wijaya , Wijono Sutarto 
Published: January 2024

Abstract : The purpose of the study was to determine whether there is a relationship between transformational leadership style and psychological well-being of permanent employees of the production department who work at PT. Primayudha Mandirijaya. This research uses quantitative methods and descriptive statistical analysis, with pearson product moment techniques. Determinant of non-probability sampling technique with a total sample of 83 permanent employees of the production department at PT. Primayudha Mandirijaya. The measuring instruments used in this study were the mulmultifactor leadership scale developed by Bass and Avolio (1995) and the psyho logical well-being scales developed by Ryff (1989). The results of the analysis were obtained with a number of 0,435 and a significance value of 0.000 (< p 0.05), meaning that there is a positive and significant relationship between transformational leadership style and psychological well-being in permanent employees of the production department at PT. Primayudha Mandirijaya in Ampel, Boyolali District. This means that the higher the transformational leadership style, the higher the psychological well-being, conversely, the lower the transformational leadership style, the lower the psychological well-being.

Keywords: Transformational leadership, psychological well-being, production
department

Abstrak : Tujuan dari penelitian adalah untuk mengetahui apakah ada hubungan antara gaya kepemimpinan transformasional dan kesejahteraan psikologis karyawan para karyawan tetap bagian produksi yang bekerja di PT. Primayudha Mandirijaya. Penelitian ini menggunakan metode kuantitatif dan analisa statistik deskriptif, dengan tehnik pearson product moment. Penentu teknik pengambilan sampel non probability sampling dengan jumlah sampel sebanyak 83 karyawan tetap bagian produksi di PT. Primayudha Mandirijaya. Alat ukur yang digunakan dalam penelitian ini adalah mulmultifactor leadership scale yang dikembangkan oleh Bass dan Avolio (1995) dan psyhological well-being scales dikembangkan oleh Ryff (1989). Hasil analisa diperoleh dengan angka 0,435 dan nilai signifikansi 0,000 (< p 0,05) artinya terdapat hubungan positif dan signifikan antara gaya kepemimpinan transformasional dan kesejahteraan psikologis pada karyawan tetap bagian produksi di PT. Primayudha Mandirijaya di Ampel Kab. Boyolali. Artinya semakin tinggi gaya kepemimpinan transformasional maka semakin tinggi pula kesejahteraan psikologis, sebaliknya semakin rendah gaya kepemimpinan transformasional maka semakin rendah juga kesejahteraan psikologis.

Kata Kunci : Kepemimpinan transformasional, kesejahteraan psikologis, departemen produksi
Establishment of a mandible defect model in rabbits infected with multiple bacteria and bioinformatics analysis
Authors: Zhao Yuan , Su Jun , Xu Chong-yan , Li Yan-bo , Hu Tong , Li Yi , Yang Li , Zhao Qiang , Zhang Wen-yun 
Published: January 2024

Objective: A model of chronic infectious mandibular defect (IMD) caused by mixed infection with Staphylococcus aureus and Pseudomonas aeruginosa was established to explore the occurrence and development of IMD and identify key genes by transcriptome sequencing and bioinformatics analysis.Methods:S. aureus and P. aeruginosa were diluted to 3 × 108 CFU/mL, and 6 × 3 × 3 mm defects lateral to the Mandibular Symphysis were induced in 28 New Zealand rabbits. Sodium Morrhuate (0.5%) and 50 μL bacterial solution were injected in turn. The modeling was completed after the bone wax closed; the effects were evaluated through postoperative observations, imaging and histological analyses. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and protein‒protein interaction (PPI) network analyses were performed to investigate the function of the differentially expressed genes (DEGs).Results: All rabbits showed characteristics of infection. The bacterial cultures were positive, and polymerase chain reaction (PCR) was used to identify S. aureus and P. aeruginosa. Cone beam CT and histological analyses showed inflammatory cell infiltration, pus formation in the medullary cavity, increased osteoclast activity in the defect area, and blurring at the edge of the bone defect. Bioinformatics analysis showed 1,804 DEGs, 743 were upregulated and 1,061 were downregulated. GO and KEGG analyses showed that the DEGs were enriched in immunity and osteogenesis inhibition, and the core genes identified by the PPI network were enriched in the Hedgehog pathway, which plays a role in inflammation and tissue repair; the MEF2 transcription factor family was predicted by IRegulon.Conclusion: By direct injection of bacterial solution into the rabbit mandible defect area, the rabbit chronic IMD model was successfully established. Based on the bioinformatics analysis, we speculate that the Hedgehog pathway and the MEF2 transcription factor family may be potential intervention targets for repairing IMD.
“No one told me”: exploring factors influencing physical activity participation in Black Nova Scotian cancer survivors
Authors: Chiekwe C. Joy , Hamilton-Hinch Barbara , Grandy Scott A. , Fowles Jonathon , Keats Melanie R. 
Published: January 2024

BackgroundFew studies have examined differences in cancer survivors' physical activity and exercise behaviors by race/ethnicity and none have explored this relationship in a Canadian context.PurposeTo explore the underlying factors that influence physical activity and exercise participation in African, Caribbean, and Black (ACB) cancer survivors.MethodsSemi-structured interviews were conducted with eight ACB cancer survivors residing in Nova Scotia, Canada. Interviews broadly explored three main topics: (1) Exploring physical activity and exercise knowledge; (2) Barriers and facilitators to physical activity and exercise; and (3) Perceptions of cancer specific exercise trials and programs. Participant interviews were audio-recorded and transcribed verbatim and analyzed using an inductive thematic analysis.ResultsAn overall storyline of “No One Told Me” along with four primary themes emerged from the participant interviews. Theme 1, “the journey” describes the lack of knowledge sharing between healthcare providers and cancer survivors. Theme 2, “what's there for me” unravels participants understanding of physical activity and exercise, knowledge of available support, and guidance to maintain and/or improve wellbeing through exercise. Theme 3, “one step at a time” spotlights participants thoughts and perceptions of participating in physical activity and exercise. Theme 4, “See ME,” considers recommendations to increase awareness and participating in physical activity and exercise trials/programs and the importance of providing safe and welcoming spaces for all.DiscussionThis study offers insight into the barriers confronted by ACB cancer survivors when considering participation in physical activity and exercise. Participants were largely aware of the benefits of being physically active but described a lack of perceived knowledge sharing and welcoming spaces. Further investigation is needed to identify strategies to improve representation of ACB cancer survivors in clinical exercise studies and community, cancer-specific exercise programming.
Microbiota affects mitochondria and immune cell infiltrations via alternative polyadenylation during postnatal heart development
Authors: Liu Xiang , Shao Yijia , Han Linjiang , Zhu Yuanting , Tu Jiazichao , Ma Jianrui , Zhang Ruyue , Yang Zhen , Chen Jimei 
Published: January 2024

There is a growing body of evidence supporting the significant impact of microbiota on heart development. Alternative polyadenylation (APA) is a crucial mechanism for gene expression regulation and has been implicated in postnatal heart development. Nonetheless, whether microbiota can influence postnatal heart development through the regulation of APA remains unclear. Therefore, we conducted APA sequencing on heart tissues collected from specific pathogen-free (SPF) mice and germ-free (GF) mice at three different developmental stages: within the first 24 h after birth (P1), 7-day-old SPF mice, and 7-day-old GF mice. This approach allowed us to obtain a comprehensive genome-wide profile of APA sites in the heart tissue samples. In this study, we made a significant observation that GF mice exhibited noticeably longer 3ʹ untranslated region (3ʹ UTR) lengths. Furthermore, we confirmed significant alterations in the 3ʹ UTR lengths of mitochondria-related genes, namely Rala, Timm13, and Uqcc3. Interestingly, the GF condition resulted in a marked decrease in mitochondrial cristae density and a reduction in the level of Tomm20 in postnatal hearts. Moreover, we discovered a connection between Rala and Src, which further implicated their association with other differentially expressed genes (DEGs). Notably, most of the DEGs were significantly downregulated in GF mice, with the exceptions being Thbs1 and Egr1. Importantly, the GF condition demonstrated a correlation with a lower infiltration of immune cells, whereby the levels of resting NK cells, Th17 cells, immature dendritic cells, and plasma cells in GF mice were comparable to those observed in P1 mice. Furthermore, we established significant correlations between these immune cells and Rala as well as the related DEGs. Our findings clearly indicated that microbiota plays a vital role in postnatal heart development by affecting APA switching, mitochondria and immune cell infiltrations.
Students’ motivation to learn English: the importance of external influence on the ideal L2 self
Authors: Ishida Ayame , Manalo Emmanuel , Sekiyama Takashi 
Published: January 2024

IntroductionThe ideal second/foreign language (L2) self, a concept in second-language acquisition theory, is a learner’s future vision about their language ability. Research on the ideal L2 self is important for finding ways to improve motivation for learning a new language, foreign language teaching strategies, and personalization of instruction for individuals with different backgrounds and aspirations. The present study aimed to better understand the factors that might influence the ideal L2 self among Japanese elementary school students. While there have been numerous studies about the ideal L2 self in English language learning, few have focused on elementary school students’ motivation to learn English.MethodsData were collected from 225 4th- and 6th-grade elementary school students in Japan. The data were analyzed by performing t-tests and a hierarchical linear regression to understand the relationships between the pertinent variables and the ideal L2 self.ResultsThe analysis results revealed three characteristics of the relationship between elementary school students’ English language skills and their ideal L2 selves. First, the results of the t-test suggested that international travel and English cram school experiences were differentiating factors for the ideal L2 self, suggesting that the groups of participants with and without those factors (experiences) had significantly different ideal L2 selves. Second, the experience of living abroad was not a significant differentiating factor for the ideal L2 self. Third, the results of the regression analysis suggested that school, home, and foreign media influences are significant contributors to the formation of the ideal L2 self. These factors may therefore subsume international travel and English cram school experiences.DiscussionBy understanding the factors that contribute to the ideal L2 self, educators and policymakers can strive to establish a more effective environment to improve students’ motivation to learn English.
Dose-response association between 24-hour total movement activity and testosterone deficiency in adult males
Authors: Wu Shenghao , Chen Wu , Cai Yaoyao , Xia Weiting 
Published: January 2024

Background and objectivesPrevious studies on the relationship between physical activity and testosterone are limited and controversial. Hence we investigated whether high level of physical activity is associated with a low risk of testosterone deficiency (TD).MethodsThis cross-sectional analysis was conducted in a representative sample of US adult males who participated in the 2011-2014 cycle of the National Health and Nutrition Examination Survey (NHANES). We used the monitor independent movement summary (MIMS) to assess activity intensity, a novel physical activity metrics developed using raw data collected by accelerometers. Multivariable regression and smooth curve fitting was used to describe the relationships between physical activity and TD, and segmented regression model were used to analyze the threshold effect between them. Sensitivity analysis was conducted using interaction and stratified analysis.ResultsA U-shaped relationship between daily MIMS units and risk of TD was observed. The optimal value of daily MIMS units for the lowest risk of TD was 14.77 (×103), the risk of TD decreased by 5% in patients per unit increase of daily MIMS units when daily MIMS units &lt;14.77 (×103) (adjusted OR = 0.95, 95%CI: 0.91, 0.99), but increased by 12% per unit increase of daily MIMS units when daily MIMS units ≥14.77 (×103) (adjusted OR = 1.12, 95%CI: 1.01, 1.23). In sensitivity analyses, the threshold effect was also similar according to baseline characteristics (P-interaction &gt;0.05).ConclusionIn a nationally representative sample of US adult males, light to moderate intensity physical activity is associated with a lower odds of TD, while high-intensity physical activity is associated with a higher risk of TD.
Disentangling associations between multiple environmental exposures and all-cause mortality: an analysis of European administrative and traditional cohorts
Authors: Dimakopoulou Konstantina , Nobile Federica , de Bont Jeroen , Wolf Kathrin , Vienneau Danielle , Ibi Dorina , Coloma Fabián , Pickford Regina , Åström Christofer , Sommar Johan Nilsson , Kasdagli Maria-Iosifina , Souliotis Kyriakos , Tsolakidis Anastasios , Tonne Cathryn , Melén Erik , Ljungman Petter , de Hoogh Kees , Vermeulen Roel C. H. , Vlaanderen Jelle J. , Katsouyanni Klea , Stafoggia Massimo , Samoli Evangelia 
Published: January 2024

BackgroundWe evaluated the independent and joint effects of air pollution, land/built environment characteristics, and ambient temperature on all-cause mortality as part of the EXPANSE project.MethodsWe collected data from six administrative cohorts covering Catalonia, Greece, the Netherlands, Rome, Sweden, and Switzerland and three traditional cohorts in Sweden, the Netherlands, and Germany. Participants were linked to spatial exposure estimates derived from hybrid land use regression models and satellite data for: air pollution [fine particulate matter (PM2.5), nitrogen dioxide (NO₂), black carbon (BC), warm season ozone (O3)], land/built environment [normalized difference vegetation index (NDVI), distance to water, impervious surfaces], and ambient temperature (the mean and standard deviation of warm and cool season temperature). We applied Cox proportional hazard models accounting for several cohort-specific individual and area-level variables. We evaluated the associations through single and multiexposure models, and interactions between exposures. The joint effects were estimated using the cumulative risk index (CRI). Cohort-specific hazard ratios (HR) were combined using random-effects meta-analyses.ResultsWe observed over 3.1 million deaths out of approximately 204 million person-years. In administrative cohorts, increased exposure to PM2.5, NO2, and BC was significantly associated with all-cause mortality (pooled HRs: 1.054, 1.033, and 1.032, respectively). We observed an adverse effect of increased impervious surface and mean season-specific temperature, and a protective effect of increased O3, NDVI, distance to water, and temperature variation on all-cause mortality. The effects of PM2.5 were higher in areas with lower (10th percentile) compared to higher (90th percentile) NDVI levels [pooled HRs: 1.054 (95% confidence interval (CI) 1.030–1.079) vs. 1.038 (95% CI 0.964–1.118)]. A similar pattern was observed for NO2. The CRI of air pollutants (PM2.5 or NO2) plus NDVI and mean warm season temperature resulted in a stronger effect compared to single-exposure HRs: [PM2.5 pooled HR: 1.061 (95% CI 1.021–1.102); NO2 pooled HR: 1.041 (95% CI 1.025–1.057)]. Non-significant effects of similar patterns were observed in traditional cohorts.DiscussionThe findings of our study not only support the independent effects of long-term exposure to air pollution and greenness, but also highlight the increased effect when interplaying with other environmental exposures.
Identification and validation of hub genes and molecular classifications associated with chronic myeloid leukemia
Authors: Zhong Fangmin , Yao Fangyi , Xu Shuai , Zhang Jing , Liu Jing , Wang Xiaozhong 
Published: January 2024

BackgroundChronic myeloid leukemia (CML) is a kind of malignant blood tumor, which is prone to drug resistance and relapse. This study aimed to identify novel diagnostic and therapeutic targets for CML.MethodsDifferentially expressed genes (DEGs) were obtained by differential analysis of the CML cohort in the GEO database. Weighted gene co-expression network analysis (WGCNA) was used to identify CML-related co-expressed genes. Least absolute shrinkage and selection operator (LASSO) regression analysis was used to screen hub genes and construct a risk score model based on hub genes. Consensus clustering algorithm was used for the identification of molecular subtypes. Clinical samples and in vitro experiments were used to verify the expression and biological function of hub genes.ResultsA total of 378 DEGs were identified by differential analysis. 369 CML-related genes were identified by WGCNA analysis, which were mainly enriched in metabolism-related signaling pathways. In addition, CML-related genes are mainly involved in immune regulation and anti-tumor immunity, suggesting that CML has some immunodeficiency. Immune infiltration analysis confirmed the reduced infiltration of immune killer cells such as CD8+ T cells in CML samples. 6 hub genes (LINC01268, NME8, DMXL2, CXXC5, SCD and FBN1) were identified by LASSO regression analysis. The receiver operating characteristic (ROC) curve confirmed the high diagnostic value of the hub genes in the analysis and validation cohorts, and the risk score model further improved the diagnostic accuracy. hub genes were also associated with cell proliferation, cycle, and metabolic pathway activity. Two molecular subtypes, Cluster A and Cluster B, were identified based on hub gene expression. Cluster B has a lower risk score, higher levels of CD8+ T cell and activated dendritic cell infiltration, and immune checkpoint expression, and is more sensitive to commonly used tyrosine kinase inhibitors. Finally, our clinical samples validated the expression and diagnostic efficacy of hub genes, and the knockdown of LINC01268 inhibited the proliferation of CML cells, and promoted apoptosis.ConclusionThrough WGCNA analysis and LASSO regression analysis, our study provides a new target for CML diagnosis and treatment, and provides a basis for further CML research.
Trypanosoma cruzi antigen detection in blood to assess treatment efficacy and cure in mice models of Chagas disease
Authors: de Araujo Fernanda Fortes , Nagarkatti Rana , Mazzeti Ana Lia , Gonçalves Karolina Ribeiro , Figueiredo Diniz Lívia de , Campos do Vale Isabela , Martins-Filho Olindo Assis , Debrabant Alain , Bahia Maria Terezinha , Teixeira-Carvalho Andréa 
Published: January 2024

IntroductionChagas disease (CD) is caused by the protozoan parasite Trypanosoma cruzi. Although endemic mainly in Latin America, CD has become a global public health problem due to migration of infected individuals to non-endemic regions. Despite progress made in drug development, preclinical assays for drug discovery are required to accelerate the development of new drugs with reduced side effects, which are much needed for human treatment.MethodsWe used a cure model of infected mice treated with Fexinidazole (FZ) to further validate a novel Enzyme Linked Aptamer (ELA) assay that detects parasite biomarkers circulating in the blood of infected animals.ResultsThe ELA assay showed cure by FZ in ~71% and ~77% of mice infected with the VL-10 and Colombiana strains of T. cruzi, respectively. The ELA assay also revealed superior treatment efficacy of FZ compared to Benznidazole prior to immunosuppression treatment.DiscussionOur study supports the use of ELA assay as an alternative to traditional serology or blood PCR to assess the efficacy of antichagasic drugs during their preclinical phase of development. Further, the combination of high sensitivity and ease of use make this parasite antigen detection assay an attractive new tool to facilitate the development of much needed new therapies for CD.
Sub-optimal temperatures lead to altered expression of stress-related genes and increased ‘Candidatus Liberibacter solanacearum’ accumulation in potato psyllid
Authors: Fisher Tonja W. , Munyaneza Joseph E. , Brown Judith K. 
Published: January 2024

IntroductionThe potato psyllid Bactericera cockerelli is the insect vector of the fastidious bacterium ‘Candidatus Liberibacter solanacearum’. The bacterium infects both B. cockerelli and plant species, causing zebra chip (ZC) disease of potato and vein-greening disease of tomato. Temperatures are known to influence the initiation and progression of disease symptom in the host plant, and seasonal transitions from moderate to high temperatures trigger psyllid dispersal migration to facilitate survival.Methods‘Ca. L. solanacearum’ -infected and uninfected psyllids were reared at previously established ‘permissible’, optimal, and ‘non-permissible’ and temperatures of 18°C, 24°C, and 30°C, respectively. Gene expression profiles for ‘Ca. L. solanacearum’-infected and -uninfected adult psyllids reared at different temperatures were characterized by Illumina RNA-Seq analysis. Bacterial genome copy number was quantified by real-time quantitative-PCR (qPCR) amplification.ResultsRelative gene expression profiles varied in psyllids reared at the three experimental temperatures. Psyllids reared at 18°C and 30°C exhibited greater fold-change increased expression of stress- and ‘Ca. L. solanacearum’ invasion-related proteins. Quantification by qPCR of bacterial genome copy number revealed that ‘Ca. L. solanacearum’ accumulation was significantly lower in psyllids reared at 18°C and 30°C, compared to 24°C.DiscussionTemperature is a key factor in the life history of potato psyllid and multiplication/accumulation of ‘Ca. L. solanacearum’ in both the plant and psyllid host, influences the expression of genes associated with thermal stress tolerance, among others, and may have been instrumental in driving the co-evolution of the pathosystem.