bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green Nanochemistry: Argyreia Nervosa-Assisted Synthesis of Cu-Ag Bimetallic Nanoparticles with Enhanced Antibacterial Properties
Authors: Kalakonda Parvathalu , Kathi Rajitha , Ligory Merlinsheeba Gali , Dabbeta Naveen Kumar , Madipoju Naveen Kumar , Mynepally. Soujanya Lakshmi , Morampudi Vijay , Banne Sreenivas , Mandal Pritam , Savu Ramu Naidu , Khanam Sarvani Jowhar , Banavoth Murali , Eve Nainavinodini Sudarsanam , Podila Bala Bhaskar 
Published: November 2023

Abstract
Scientist have demonstrated substantial interest in the biosynthesis of metallic nanoparticles, particularly for their applications in the fields of bio-nanotechnology and medicine. Our study specifically explores the biosynthesis of copper-silver bimetallic nanoparticles (Cu-Ag BMNPs) using Argyreia Nervosa (AN) plant leaf green extract as a versatile agent for capping, reducing, and stabilizing. This biosynthesis method is characterized by its simplicity and cost-effectiveness, utilizing silver nitrate (AgNO3) and cupric oxide (CuO) as precursor materials. Our comprehensive characterization of the Cu-Ag BMNPs, employing techniques such as X-Ray Diffraction (XRD), UV-Vis Spectrometry, Scanning Electron Microscopy (SEM), Zeta Sizer, and Fourier Transformed Infrared Spectrometry (FTIR), unveiled important structural and compositional details. The FTIR results confirmed the successful removal of organic and inorganic impurities, findings supported by the XRD data. To evaluate the antimicrobial properties of the Cu-Ag BMNPs, we conducted disk diffusion and Minimum Inhibitory Concentration (MIC) assays against Escherichia coli (E. coli), with results compared to the standard Gentamicin antibiotic. These assays showcased the improved antimicrobial activity of Cu-Ag bimetallic nanoparticles, highlighting their synergistic effect, characterized by high MIC values and a broad zone of inhibition in the disc diffusion tests against E. coli. These results emphasize the significant antibacterial potential of the synthesized BMNPs, with a medicinal plant Argyreia Nervosa leaf extract playing a pivotal role in enhancing this activity.
Response mechanisms of Candida tropicalis incubated with a dilute acid hydrolysate from corn stover
Authors: Zhang zhengyue , Wu Lan , Li Qi , Shu Baichao , Li Dang , Chen Yulei , Tang Jiaye , Long Siyi , Liao Jie , Zhao Yifan , Wang Hanyu , Ma Menggen 
Published: November 2023

Abstract
Understanding the cellular response mechanisms of Candida tropicalis is crucial to biofuel production from corn stover, and targeted genetic modification of a C. tropicalis fermentation strain can improve the biofuel yield. In this report, metabolomic analysis of a hydrolysate obtained from dilute acid hydrolysis of corn stover identified 1,469 substances, including sugars, aldehydes, acids and phenols. In the presence of the corn stover hydrolysate, cell growth was inhibited. Moreover, subcellular observations revealed that C. tropicalis SHC-03 accumulated reactive oxygen species and maintained endoplasmic reticulum homeostasis when incubated with this hydrolysate. For detoxification of byproducts in the hydrolysate that inhibit cell growth and survival, genes associated with reduced glutathione, ergosterol, and ubiquinone-n biosynthesis and misfolded protein and fatty acid degradation were upregulated upon incubation with the hydrolysate. These results will help guide genetic modifications that increase the intracellular synthesis of NADH/NADPH and acetyl-CoA for ergosterol and fatty acid accumulation to improve tolerance to hydrolytically toxic byproducts and accelerate industrial production of bioethanol and other bioproducts.
WITHDRAWN: Kinetics of microbial digestion of leachate generated from municipal solid waste
Authors: Bhambore Nandini , Kumar M. Suresh 
Published: November 2023

Abstract
The study addresses the challenge of leachate generation and explores various treatment processes. Specifically, it focuses on the characterization of Bhandewadi municipal solid waste leachate and evaluates its biodegradability. This evaluation involves the removal of organic and inorganic components under both anaerobic and aerobic conditions at different inoculum ratios. Additionally, the study employs mathematical kinetics models to optimize and validate the data obtained. Two sets of experiments were conducted using 25%, 50%, and 100% (raw leachate) inoculum ratios by combining leachate with inoculum seed. In the aerobic environment, a COD removal efficiency of 75% was achieved within 14 days, while in the anaerobic environment, 77% removal was observed over 18 days. To model biogas production, first-order, modified Gompertz, and logistic function models were employed and fitted to the experimental data of the 25% anaerobic set. The logistic function model proved to be the most compatible with the experimental data for this scenario. Furthermore, mathematical kinetics were also applied to validate the COD removal efficiency in the 25% inoculum ratio of the aerobic study. In this case, the first-order equation was found to be the best fit for the data. Overall, the study indicates that lower inoculum concentrations can also result in better treatment performance, particularly with a 25% inoculum ratio displaying the highest degradation efficiency under both aerobic and anaerobic conditions.
Transgenerational Impacts of Oxidative Damage Induced by Prenatal Ethanol Exposure on Spatial Learning/Memory and BDNF in the Hippocampus of Male Rats
Authors: Ghahremanlou Mousa Shaabani , Hojati Vida , Sharafi Shahram 
Published: November 2023

Abstract
Background and Aim:
Alcohol use during pregnancy causes damage to normal fetal development and induces oxidative stress (OS), and intervenes in the expression of neurotrophic factors (NTFs).
Materials and Methods
A rat model of fetal alcohol spectrum disorder (FASD) was initially generated to reflect on the deficits in the first generation, and then those transmitted via the male germline to the unexposed male ones. The pregnant rats were thus divided into four groups, namely, the control group (CTRL) receiving only distilled water, and three groups being exposed to ethanol (20%, 4.5 g/kg) by oral gavage, during the first 10-days gestation (FG), the second 10-days gestation (SG), and the entire gestation (EG) periods. Half of the male offspring were then subjected to the Morris water maze (MWM) test on the 30 postnatal days. The other half was further recruited to evaluate the activity of the antioxidant enzymes, including glutathione peroxidase (GPx), superoxide dismutase (SOD), and malondialdehyde (MDA), in the hippocampus. Ultimately, the qRT-PCR was practiced to measure the BDNF gene expression.
Results
Prenatal ethanol exposure caused spatial learning/memory deficits during the SG and EG in both generations. The elevated MDA levels, along with the decreased GPx and SOD activity and BDNF gene expression occurred all through the SG and EG of the first filial generation (F1), and the reduction in the GPx and SOD activity together with the BDNF gene expression was only observed in the EG of the second filial generation (F2) of the male rats.
Conclusion
OS could play a leading role in the diminished BDNF gene expression as well as the spatial learning/memory decline induced by prenatal ethanol exposure.
Comparative Analysis of Surgical Techniques in the Management of Chronic Subdural Hematomas and Risk Factors for Poor Outcomes.
Authors: HOUNKPATIN Stachys , STIERER Marine , Paul FRECHON , Frederick RAULT , KELANI Aminath , EMERY Evelyne , GABEREL Thomas , LECLERC Arthur 
Published: November 2023

Abstract
Background. Chronic subdural hematomas (CSDH) are increasingly prevalent, especially among the elderly. Surgical intervention is essential in most cases. However, the choice of surgical technique, either craniotomy or burr-hole opening, remains a subject of debate. Additionally, the risk factors for poor long-term outcomes following surgical treatment remain poorly described.
Methods. This article presents a 10-year retrospective cohort study conducted at a single center that aimed to compare the outcomes of two common surgical techniques for CSDH evacuation: burr hole opening and craniotomy. The study also identified risk factors associated with poor long-term outcome, which was defined as an mRS score ≥ 3 at 6 months.
Results. This study included 582 adult patients who were surgically treated for unilateral CSDH. Burr-hole opening was performed in 43% of the patients, while craniotomy was performed in 57%. Recurrence was observed in 10% of the cases and postoperative complications in 13%. The rates of recurrence, postoperative complications, death and poor long-term outcome did not differ significantly between the two surgical approaches. Multivariate analysis identified postoperative general complications, recurrence, and preoperative mRS score ≥ 3 as independent risk factors for poor outcomes at 6 months.
Conclusion. Burr-hole opening is as effective and less invasive than craniotomy and should be preferred. Although chronic subdural hematoma is often considered benign, general complication and recurrence are significant long-term prognostic factors that should not be overlooked. Our results highlight the importance of preventing postoperative complications through early mobilization and avoiding the prescription of corticosteroids in this elderly population.
Evaluation of the Impact of Rural Streetscape Enhancement on Relaxation-Arousal Responses Based on EEG and VR
Authors: Ren Hongguo , Wang Yujun , Zhang Jing , Wang Qingqin , Zheng Ziming 
Published: November 2023

Abstract
Targeting the issue of emotional deficiency in rural human environments, this study combines electroencephalogram (EEG) and virtual reality (VR) technologies thus we choose as experimental images the current situation scenes of five rural main street nodes as well as the comparative transformation scenes. It then assesses the subjects' subjective cognition and level of relaxation-arousal responses using the α/β index in the EEG data and the three subjective scale indexes of "Interested," "Comfortable," and "Sense of vitality." The study's findings demonstrated that: 1. All three scores increased in the transformation scenarios, and subjects' subjective assessments varied significantly across all five sets of scenes. 2. Every transformation scene showed relaxation responses in comparison to status quo scenes, except for the T8 electrode, which displayed the only arousal response in the second set of scenes, out of all the α/β index values that were substantially different. 3. The Pz electrode in the parietal lobe area was found to be the most sensitive to the visual response to the green landscape and the form of the building façade along the street, and the T8 electrode in the right temporal lobe area was the most sensitive to the response to the overall perception of the surrounding environment, according to a comparison of the longitudinal α/β values. More options for rural streetscape design as well as fresh insights and methodologies for assessing the rural human environment in the future are anticipated from this study.
MUS81 Inhibits Cell Proliferation, Migration and Invasion in Breast Cancer
Authors: Xie Rong , Su Xinyu , Yang Yonggang 
Published: November 2023

Abstract
Researchers have demonstrated that MUS81 is a powerful tumor suppressor, and plays in important role in DNA damage repair and maintenance of chromosomal stability in mice. To explore its role and expression pattern in human breast cancer (BC), which currently are unknown, we carried out this study to examine the correlation between MUS81 expression and the proliferation and invasiveness of BC cells. The expression of MUS81 in BC was predicted by bioinformatics. QRT-PCR and Western Blot were used to determine MUS81 expression levels in BC. Stable transfection of MUS81 overexpression lentivirus (MUS81) or MUS81 interference lentivirus (MUS81 RNAi) to establish its overexpression or knockdown in BC cells. CCK8 and colony formation experiments were used to determine the proliferation ability of the cells; scratch and transwell experiments were performed to detect the invasiveness of the cells. MUS81 overexpression cells were inoculated subcutaneously at the dorsal flanks of nude mice to form subcutaneous xenografts and evaluate the functional impact of MUS81 on the growth of BC. The expression of MUS81 and Ki-67 in xenografts were also detected by immunohistochemistry. Both MUS81 mRNA and protein expression levels were reduced significantly in BC tissues and cells compared with the corresponding normal tissues and MCF-10A. In vitro experiments indicated overexpression or silencing of MUS81 respectively decreased and facilitated proliferation and invasion of BC cells than control groups. In vivo experiment revealed a significant reduction in the growth of BC cells and tumor weight of their formed subcutaneous xenografts in nude mice was noted upon overexpression of MUS81. The expression level of Ki 67 was also dramatically reduced following the overexpression of MUS81. MUS81 was significantly low-expressed in breast cancer, and the low expression was correlated with the enhanced proliferation and aggressiveness of breast cancer cells, suggesting that MUS81 as a candidate tumor suppressor gene.
A comparison of coffee effects on vigilance and attention in pilots across simple and complex lab environments
Authors: Bergen Geertje van , Miltenburg Maykel van , Stiphout Ruud van , Drongelen Alwin van , Riesenbeck Lea , Sieters Jane , Dijksterhuis Garmt , Vingerhoeds Monique , Aarts Esther 
Published: November 2023

Abstract
Professionally realistic multi-task environments, such as the NASA Multi-Attribute Task Battery (MATB-II), are employed for measuring vigilance and attention in professionals. However, it is unclear whether well-known intervention effects on performance during simple lab-based tasks, such as those of caffeine, also translate to these more realistic working situations. In a preregistered, double-blind, randomized, controlled repeated-measures experiment (https://osf.io/2zubx), we compared the performance of thirty-five civil pilots during vigilance- and attention-related tasks in simple (psychomotor vigilance task; auditory oddball detection) versus multitask environments (MATB-II system monitoring; MATB-II communications) after consuming regular vs. decaffeinated coffee. For vigilance tasks, no coffee intervention effects were found. Instead, a reversed task repetition effect was found, with participants being slower in session 2 in the simple task environment, but faster in session 2 in the complex environment. For attention-related tasks, regular coffee improved performance accuracy in the simple, but not the multitask environment. Coffee versus decaf effects in the simple task environment did not correlate with those in the complex task environment, neither for vigilance nor for selective attention. However, an experiment-wide increase in sleepiness was attenuated if participants drank regular coffee in the second session. This finding was supported by heart rate and eye blink measures. Results suggest that intervention-related findings do not easily translate to different vigilance- and attention-related tasks if task environments differ in complexity. The MATB-II multi-task environment, in its current form, is perhaps more suitable for assessing intervention effects on physiological measures of fatigue and vigilance than on cognitive performance.
Risk factors associated with neonatal mortality among neonates admitted to neonatal intensive care unit of the university teaching hospital in Lusaka, Zambia: A retrospective analysis
Authors: Tembo Deborah , Bessing Barnabas , Abobo Francis D N , Kaonga Patrick , Jacobs Choolwe 
Published: November 2023

Abstract
Background: Globally, several children die shortly after birth and many more of them within the first 28 days of life. Sub-Sharan Africa accounts for almost half (43%) of the global neonatal death with slow progress in reduction. These neonatal deaths are associated with lack of quality care at or immediately after birth and in the first 28 days of life. This study aimed to determine the trends and risk factors of neonatal mortality in a major referral hospital in Lusaka, Zambia.
Methods: We conducted retrospective analysis involving all neonates admitted in the University Teaching Hospital Neonatal Intensive Care Unit (UTH-NICU) in Lusaka from January 2018-December 2019 (N=2340). We determined the trends and assessed the factors associated with neonatal mortality using Generalized Linear Models (GLM) with a Poisson distribution and log link function.
Results: Overall, the neonatal mortality was 40.2% (95% CI: 38.0-42.0) per 1000 live births for the two-year period with a slight decline in mortality rate from 42.9% (95% CI: 40.0-46.0) in 2018 to 37.3% (95% CI: 35.0-40.0) in 2019. In a final multivariable model, home delivery (ARR: 1.70, 95% CI: 1.46-1.96), preterm birth (ARR: 1.59, 95% CI: 1.36-1.85), congenital anomalies (ARR: 1.59, 95% CI: 1.34-1.88), low birthweight (ARR: 1.57, 95% CI: 1.37-1.79), and health centre delivery (ARR: 1.48, 95% CI: 1.25-1.75) were independently associated with increase in neonatal mortality. Conversely, hypothermia (ARR: 0.36, 95% CI: 0.22-0.60), antenatal attendance (ARR: 0.76, 95% CI: 0.68-0.85), and one-day increase in neonatal age (ARR: 0.96, 95% CI: 0.95-0.97) were independently associated with reduction in neonatal mortality.
Conclusion: In this hospital-based study, neonatal mortality was high compared to the national and global targets. While non-modifiable risk factors were associated with increase in neonatal mortality, proper management of modifiable risk factors resulted in reduced neonatal mortality. Early identification and interventions to reduce the impact of both non-modifiable and modifiable risks factors of neonatal mortality in Zambia are important.
P5C inhibit T cell glycolysis in prostate cancer microenvironment by SHP1/PKM2/LDHB axis
Authors: Chang Lei , Li Guohao , Jiang Shan , Li Jie , Yang Jin , Zhou Le , Song Hanrui , Deng Leyuan , Luo Zhiguo , Shah Kavita , Guo Yonglian , Yan Yutao 
Published: November 2023

Abstract
Background: Our previous studies demonstrated that 1-Pyrroline-5-carboxylate (P5C) released by prostate cancer cells inhibit T cell proliferation and function. We designed this study to further explore the influence of P5C on T cell metabolism, and produce an antibody for targeting P5C to restore the functions of T cells.
Method: We obtained SHP1 from T cells through co-immunoprecipitation and analyzed the proteins that were co-immunoprecipitated with SHP1 using liquid chromatography mass spectrometry (LC/MS-MS). The influence of P5C on T cells metabolism also could be detected by LC/MS-MS. Seahorse XF96 analyzer was used to identify the effect of P5C on T cells glycolysis. Then we designed and produced an antibody for targeting P5C by monoclonal technique, and verified its effectiveness to restore the function of T cells in vitro and in vivo.
Result: PKM2 and LDHB bind SHP1 in T cells, and P5C could increase the levels of p-PKM2 while have no effect on the levels of PKM2 and LDHB. We further found that P5C influences T cell energy metabolism and carbohydrate metabolism. P5C also inhibits the activity of PKM2 and decrease the content of intracellular lactic acid while increasing the activity of LDH. Using seahorse XF96 analyzer, we confirmed that P5C remarkably inhibits glycolysis in T cells. We produced an antibody for targeting P5C by monoclonal technique, and verified that the antibody could oppose the influence of P5C to restore the process of glycolysis and function in T cells. Meanwhile, the antibody also inhibits the growth of prostate tumors in animal model.
Conclusion: Our study revealed that P5C inhibits the process of glycolysis in T cells by targeting SHP1/PKM2/LDHB complexes. Moreover, it is important that the antibody for targeting P5C could restore the function of T cell and inhibit the growth of prostate tumors.
Reduced Training Data for Robust Ultrasound Signal Interpretation by Neural Networks
Authors: Rus Janez , Fleury Romain 
Published: November 2023

Abstract
We introduce an experimental setup to generate large datasets of ultrasonic signals relevant for quality inspection. A reconfigurable defect is induced by a heating laser projecting a short line on a shape memory polymer foil, which has a special property that its Young’s modulus and ultrasound attenuation level can locally be controlled by its temperature field. Ultrasound is generated by a laser pulse at one fixed position and detected by a laser vibrometer at another fixed position for 64 different defect positions and 3 different configurations of the specimen. The obtained diversified datasets are used to optimize the network architecture for the interpretation of ultrasound signals. We study the robustness of the model in cases of reduced and dissimilar training datasets. In our first study, we classify the specimen configurations with the defect position being the disturbing parameter. The model shows high performance on a dataset of signals obtained at all the defect positions, even if only trained on a completely different dataset containing signals obtained at few defect positions. In our second study, we perform precise defect localization. The model becomes robust to the changes in the specimen configuration when a reduced dataset, containing signals obtained at two different specimen configurations, is used for the training process. These conclusions show the great potential of the demonstrated machine learning algorithm for industrial quality control. High-volume products (simulated by a reconfigurable specimen in our work) can be rapidly tested on the production line using this single-point and contact-free ultrasonic method.
Reducing Umbilical Catheter Migration Rates By Using a Novel Securement Device
Authors: Scottoline Brian , Perl Juliana , Crabtree-Beach Tanya , Olyaei Amy , Hedges Madeline , Jordan Brian 
Published: November 2023

Abstract
Objective
This study evaluates the effectiveness of a novel device, LifeBubble, in reducing umbilical cord catheter (UC) migration and associated complications in neonates.
Study Design:
A retrospective review was performed at Oregon Health & Science University's NICU (2019–2021) to compare standard adhesive securement with LifeBubble. The primary outcomes were UC migration, discontinuation due to malposition, and CLABSI incidence. Statistical analyses included Wilcoxon Rank Sum, Pearson Chi Square tests, and logistic regression adjusted for weight and age.
Results
Among 119 neonates (89 LifeBubble, 106 adhesive), LifeBubble significantly reduced UC migration > 1 vertebral body (5.6% vs 44%) and UVC discontinuation due to malposition (5.6% vs 39%). The number needed to treat (NNT) to prevent one instance of these outcomes was 3. No significant difference was found in UAC outcomes or CLABSI rates.
Conclusion
LifeBubble effectively reduces UC migration and premature discontinuation, indicating its potential in enhancing neonatal care and safety.