bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antifungal and antibacterial activities of Lippia callicarpifolia Kunth essential oils and influence of phenological stage on chemical composition
Authors: Villagómez-Guzmán Ana Karen , García-Bores Ana María , López-Perez Miriam Arlette , Estrella-Parra Edgar Antonio , Avila-Acevedo José Guillermo , Cansino Roció Gómez , Camilo Patricia Bustamante , Hernández-Delgado Claudia Tzasna 
Published: November 2023

Abstract

Background: Lippia callicarpifolia Kunth (Verbenaceae) is an aromatic perennial shrub popularly known as “flor de aire”, “mostranzo”, and “rosa de castilla” in the community of Tonatico, State of Mexico, Mexico. This study examined the influence of phenological stage on the chemical composition of essential oils (EOs) obtained from aerial parts collected at three different developmental periods and the impact on their antimicrobial activity against bacteria and yeasts of medical importance, including microorganisms isolated from clinical cases.
Methods: GC‒MS, retention indices, and standard compounds were employed to identify 38 constituents. The antimicrobial activity of the EOs was evaluated following the Clinical and Laboratory Standard Institute M100 guidelines (2018).
Results: During the pre-flowering stage (September), the essential oil of fresh leaves/stems (LSEP) was composed mainly of β-caryophyllene. In contrast, β-phellandrene, β-caryophyllene, and α-pinene were the major compounds in the EOs of leaves/stems and flowers collected during full bloom in November (LNOV and FNOV, respectively) and at the end the flowering stage in February (LFEB and FFEB, respectively). All EOs studied exhibited antibacterial and anticandidal activities. Determination of minimal inhibitory concentrations (MICs) and bactericidal or fungicidal concentrations (MBCs or MFCs) revealed that the most significant antimicrobial activity was observed for the essential oils LNOV and FNOV. LNOV showed the highest level of antifungal activity, significantly inhibiting the growth of Candida glabrata (with an MIC value of 0.5 mg/mL). In addition, the essential oil of flowers FNOV exhibited a broader antibacterial spectrum, effectively inhibiting the development of all gram-negative and gram-positive bacteria studied, including multidrug-resistant strains isolated from clinical cases. The LSEP, LNOV, and FNOV EOs inhibited C. glabrata biofilm formation.
Conclusions: The EOs of L. callicarpifolia Kunth may be a viable alternative source of naturally occurring antimicrobial agents for effectively combating infectious diseases that offer an affordable approach to treatment. This study examines for the first time the chemical composition of L. callicarpifolia EOs during different growth stages, including vegetative and flowering stages, along with their antimicrobial activity.
Fat-1 Gene Inhibit Hepatocarcinogenesis Induced by AKT and Ras Oncogenes in Mice
Authors: li yixian , zhao yan , liu xinxin 
Published: November 2023

Abstract
Background and Aim:
n-3 PUFAs have been shown to have multiple biological effects on cancers. The fat-1 gene encodes an n-3 desaturase that introduces a double bond at the n-3 position of the hydrocarbon chain in n-6 fatty acids to form an n-3 fatty acid. Here we investigated the anti-tumorigenesis effect of Fat-1 gene.
Methods: Mounting evidence indicates that activation of AKT and Ras pathways is a key oncogenic event in human hepatocarcinogenesis. Our previous results demonstrate that co-expression of AKT and N-Ras in the mouse liver promotes rapid hepatocarcinogenesis in vivo. Here we utilized hydrodynamic tail vein injection of Fat-1 gene to AKT/Ras hepatocarcinogenesis' model to testify its anti-tumorigenesis effect.
Results: Hydrodynamic injection of Fat-1 gene could inhibit lipogenesis and affects liver tumor development in co-expressing AKT and Ras oncogenes' mice. Molecular analysis showed that Fat-1 gene strongly inhibited the Ras/MAPK and Akt/ mTORC cell signal pathway, and significantly inhibited de novo lipogenesis by inhibition of fatty acid biosynthesis gene (FASN, ACLY) and transcription factors (LXR-β, SREBP1), which were highly elevated in AKT/Ras mice. The AKT/Ras cells lines in vitro also showed the similar results. Noticeably, although the hydrodynamic injection of fat-1 gene showed significant inhibition effect of hepatocarcinogenesis, it didn't change fatty acid profile in various tissues of mice, which is different from previous studies by using Fat-1 transgenic mice.
Conclusions Local expression of Fat-1 gene can achieve sufficient suppression of AKT/Ras-induced hepatocarcinogenesis. Thus, with multiple biological effects on various signal pathways, Fat-1 gene can prevent of hepatocarcinogenesis.
Copaiba oil’s bactericidal activity and its effects on health and zootechnical performance for Nile tilapia after oral supplementation
Authors: Mello Nicoli Paganoti , Espinoza Fernando Carlos Ramos , Claudiano Gustavo da Silva , Yunis-Aguinaga Jefferson , Carvalho Janaina Graça de Oliveira , Silva Josiane Elizabeth Almeida , de Oliveira Elaine Cristina Pacheco , de Moraes Julieta Rodini Engrácia 
Published: November 2023

Abstract

This study aimed to verify the bactericidal activity of Copaiba oil, Copaifera duckei, against the main tilapia pathogens and the effect on dietary levels of commercial Copaiba oil for 30 days on zootechnical performance, hematological, biochemical, immunological and histological parameters before and after an experimental infection with Streptococcus agalactiae in Nile tilapia. Fish were randomly distributed into 15 fiber tanks in five treatments (0%, 0.25%, 0.50%, 0.75% and 1.0%) and fed with commercial diet supplemented wit Copaiba oil for 30 days. After this period, the fish were randomly redistributed for the experimental challenge with S. agalactiae into six treatments (T0, T1, T2, T3, T4 and T5) and the fish were anesthetized, and blood samples were collected to assess hematological, biochemical, immunological and histological parameters. Copaiba oil showed bactericidal activity against Streptococcus spp. and Flavobacterium spp. in vitro. In addition, concentrations of 0.75% and 1.0% has an anti-inflammatory effect and improve hematological and immunological parameters, increasing the leukocyte numbers, albumin and serum lytic activity. Furthermore, there is an increase in the intestinal villus length and tissue damage in groups at concentrations 0.75% and 1.0% of Copaiba oil. In conclusion, oral supplementation enhanced non-specific immune parameters and digestibility in Nile Tilapia.
Advances in Online Delivery: Introducing and Optimizing a Novel Multi-Objective Function
Authors: Tunga Harinandan , Chowdhury Rupaj , Kar Samarjit , Giri Debasis , Gandomi Amir H 
Published: November 2023

Abstract
An efficient online delivery system in the dynamic landscape is a challenging task. The challenges occur due to the difficulty in generating a proper objective function that can represent theperformance of the delivery system. In this paper, we propose a novel multi-objective function that represents the utility score and time required in the delivery process. The utility score takes into consideration the number of previous orders given by a particular customer. The Time window methodology is used to achieve the two objectives. The multi-objective optimization functions are solved and compared using three multi-objective algorithms. They are Non-dominated sorting genetic algorithm-II (NSGA-II), strength Pareto evolutionary algorithm 2 (SPEA2), and indicator-based evolutionary algorithm (IBEA). The performances are compared extensively and it is found that SPEA2 gives better convergence performance. The proposed objective function minimizes the limitation of currently available methods for online delivery systems.
The efficacy and safety of androgen receptor signaling inhibitors in patients with non-metastatic castration resistant prostate cancer
Authors: Takahara Kiyoshi , Naiki Taku , Nakane Keita , Watanabe Hiromitsu , Miyake Hideaki , Koie Takuya , Yasui Takahiro , Shiroki Ryoichi 
Published: November 2023

Abstract
BACKGROUND
The treatment paradigm for non-metastatic castration resistant prostate cancer (nmCRPC) has changed in recent years. An observational multicenter study was conducted to evaluate the effectiveness of androgen receptor signaling inhibitors (ARSI) as a first-line treatment for patients with nmCRPC.
METHODS
The primary endpoint of this study was to evaluate the efficacy and safety of ARSI in native Japanese patients who received ARSI as a first-line treatment for nmCRPC. The secondary endpoint was to develop a novel system to stratify the prognoses of these patients.
RESULTS
In total, 160 patients were enrolled in this study. Within a median follow-up period of 23 months, the median overall survival (OS) was not reached, whereas the median progression-free survival was 26 months. Multivariate Cox regression analyses showed that the time to CRPC, PSA level at the initiation of nmCRPC treatment, and Geriatric Nutritional Risk Index (GNRI) were independent predictors of OS. The patients were subsequently divided into three groups as follows: Group 1, 57 patients with negative or one positive independent OS predictor; Group 2, 38 patients with two positive independent OS predictors; and Group 3, 10 patients with three independent OS predictors. The OS differed significantly among the three groups (p < 0.0001).
CONCLUSION
ARSI may provide favorable outcomes for Japanese patients with nmCRPC as a first-line treatment. Time to CRPC, PSA level at the initiation of nmCRPC treatment, and GNRI are potential predictors of OS in Japanese patients with nmCRPC who received ARSI as a first-line treatment.
Will You Become the Next Troll? A Computational Mechanics Approach to the Contagion of Trolling Behavior
Authors: Sun Qiusi , Hilbert Martin 
Published: November 2023

Abstract
Trolling is a deviant behavior that provokes emotional responses, diffuses misinformation, or otherwise disrupts on-topic discussions. This study investigates trolling behavior as a complex dynamic process. The data comprised over 13 million Reddit comments, which were classified as troll or non-troll messages using the BURT model, fine-tuned with human coding set. By employing a unique, minimally complex, maximally predictive model from statistical mechanics and information theory, i.e., epsilon-machines and transducers, we find that engaging in trolling behaviors is both self- and other-motivated. Social inputs provide more information for predicting the future behaviors of the dynamic. Social inputs are also shown to encourage and reinforce trolling behaviors among Internet users. The article serves as a showcase for the use of information-theoretic measures from dynamical systems theory to conceptualize social media processes as computational algorithms executed by collectives of human users.
Assessment of Payments For Watershed Services Policy from a Perspective of Ecosystem Services: A Case Study of the Liaohe River Basin, China
Authors: Guo Manman , Lu Xu , Ma Qing 
Published: November 2023

Abstract
Payments for watershed services (PWS), as one of the typical environmental governance policies, its efficiency assessment has become a research focus. Taking the Liaohe River in China as an example, this study try to find a more suitable assessment method for PWS. we divided its Payments for Watershed Services (PWS) policy into two types, including EC-like model (October 2008 to April 2017) and PES-like model (May 2017 to December 2021), and then by adopting the corrected uint-value transfer method based on the land use change data, we presented a comparison of the service efficiencies of the two phases based on the assessment method of ecosystem service value (ESV). According to the results, the total ESV growth in phase Ⅰ was 15.23 billion yuan, with an average value of 1.69 billion yuan per year; the numbers in phase Ⅱ was 14.86 and 3.72. The main growth spaces were concentrated along the Liaohe River mainstream and the upstream of tributaries, the main growth functions were water regulation (45.12%) and climate regulation (24.32%). The ESV growth were mainly due to the conversion of farmland into forest, grassland, wetland and water. In addition, in the PES-like model, the growth efficiency for the cities that adopted a direct payment system among counties was higher than for cities that did not take this step. In theory, the more people participate, the higher the service efficiency is, this is the main reason why the efficiency of PES is higher than that of EC in this study. Taking the ESV as the main indicator for PWS assessment, can not only conduct policy assessment and comparison, but also effectively identify land types and governance measures that generate positive externality of ecosystem.
Host immune responses associated with SARS-CoV-2 Omicron infection result in protection or pathology during reinfection depending on mouse genetic background.
Authors: Singh Gagandeep , Warang Prajakta , Diego Juan García-Bernalt , Chang Lauren , Bykov Yonina , Singh Sarabjot , Pache Lars , Cuadrado-Castano Sara , Webb Brett , Garcia-Sastre Adolfo , Schotsaert Michael 
Published: November 2023

Abstract

Rapid emergence of antigenic distinct SARS-CoV-2 variants implies a greater risk of reinfection as viruses can escape neutralizing antibodies induced by vaccination or previous viral exposure. Disease severity during COVID-19 depends on many variables such as age-related comorbidities, host immune status and genetic variation. The host immune response during infection with SARS-CoV-2 may contribute to disease severity, which can range from asymptomatic to severe with fatal outcome. Furthermore, the extent of host immune response activation may rely on underlying genetic predisposition for disease or protection. To address these questions, we performed immune profiling studies in mice with different genetic backgrounds - transgenic K18-hACE2 and wild-type 129S1 mice – subjected to reinfection with the severe disease-causing SARS-CoV-2 B.1.351 variant, 30 days after experimental milder BA.1 infection. BA.1 preinfection conferred protection against B.1.351-induced morbidity in K18-hACE2 mice but aggravated disease in 129S1 mice. We found that he cytokine/chemokine profile in B.1.351 re-infected 129S1mice is similar to that during severe SARS-CoV-2 infection in humans and is characterized by a much higher level of IL-10, IL-1β, IL-18 and IFN-γ, whereas in B.1.351 re-infected K18-hACE2 mice, the cytokine profile echoes the signature of naïve mice undergoing viral infection for the first time. Interestingly, the enhanced pathology observed in 129S1 mice upon reinfection cannot be attributed to a less efficient induction of adaptive immune responses to the initial BA.1 infection, as both K18-hACE2 and 129S1 mice exhibited similar B and T cell responses at 30 DPI against BA.1, with similar anti-BA.1 or B.1.351 spike-specific ELISA binding titers, levels of germinal center B-cells, and SARS-CoV-2-Spike specific tissue-resident T-cells. Long-term effects of BA.1 infection are associated with differential transcriptional changes in bronchoalveolar lavage-derived CD11c + immune cells from K18-hACE2 and 129S1, with K18-hACE2 CD11c + cells showing a strong antiviral defense gene expression profile whereas 129S1 CD11c + cells showed a more pro-inflammatory response. In conclusion, initial infection with BA.1 induces cross-reactive adaptive immune responses in both K18-hACE2 and 129S1 mice, however the different disease outcome of reinfection seems to be driven by differential responses of CD11c + cells in the alveolar space.
Prevalence and correlates of distress in Chinese women with benign breast disease
Authors: Gao Yan , Wang Jintao , Guo Jun , Gao Jinnan 
Published: November 2023

Abstract
Purpose: Breast cancer patients often experience high levels of psychological distress, but there is limited research on the psychological distress status of patients with benign breast diseases. This study aims to investigate the psychological distress levels and influencing factors among patients with benign breast disease (BBD).
Methods: A cross-sectional study was conducted on BBD patients admitted to a tertiary medical center in China between November 2022 and May 2023. Patient-reported distress and problems were assessed using the distress thermometer (DT) and the 42-item Problem List by the National Comprehensive Cancer Network. Descriptive statistics, chi-square test or Fisher's exact test, and multivariate logistic regression analyses were performed. Receiver operating characteristic analysis was used to evaluate the diagnostic accuracy of the DT in comparison to the Hospital Anxiety and Depression Scale (HADS) and to determine an optimal cutoff score for the DT in this specific population.
Results: Among the 255 BBD patients, the mean psychological distress score was 3.69±1.96. The prevalence of psychological distress (≥4 points) was 52%. The main factors causing psychological distress were worry or anxiety (43.5%), fear (21.2%), pain (7.1%), sleep problems (6.7%), and child care responsibilities (5.1%). Multiple logistic regression analysis revealed that age, education, employment, and BI-RADS classification were factors associated with psychological distress in BBD patients. ROC curve analyses showed that a DT cutoff score of 4 had an area under the ROC curve of 0.88 and 0.7 when compared to HADS-A and HADS-D, respectively, indicating good overall accuracy.
Conclusions: Patients with benign breast disease also experience significant psychological burden, which requires attention from healthcare professionals. Targeted health education and psychological counseling should be provided to these patients.
Digital Mapping of Coastal Landscapes Integrating Ocean-Environment Relationships and Machine Learning
Authors: Wang Kui 
Published: November 2023

Abstract
Currently, the Internet of Things (IoT) is in a premature phase. Although it is growing at a steady pace, there is still a need for further research in the field of security. In this work, the Fujian Province was selected as the study area. The climate, parent material and topographic information of the area were obtained, and the soil-landscape quantitative model was used to quantitatively obtain the relationship between the attributes of coastal sand and gravel soil. On the basis of soil type map, according to the difference of soil type elevation distribution, further predict the soil type distribution and make a map. The results show that the method can achieve more than 80% coincidence with the survey results on the scale of soil digital mapping, and can make up for the missing areas of the survey.
Causal Relationship Between Liver Iron Content, Liver Fat Percentage, Liver Volume, and Hepatocellular Carcinoma: A Mendelian Randomization Study
Authors: Han Tianrun , Li Chundi , Wang Baisen , Xu Ying , Xu Bin 
Published: November 2023

Abstract
Objective
To investigate the causal relationship between liver iron content, percent liver fat, liver volume, and hepatocellular carcinoma (HCC), using Mendelian randomization (MR).
Methods
Tools from public databases were utilized as instrumental variables to analyze the links between the exposure factors (liver iron content, percent liver fat, and liver volume) and the outcome (HCC) through two-sample Mendelian randomization analysis. The inverse-variance weighted method (IVW) was employed for the primary analysis, with MR-Egger regression, weighted median, simple mode, and weighted mode as the supplementary methods. The causal relationship among liver iron content, percent liver fat, liver volume, and HCC was evaluated using the effect indicator odds ratio (OR) and 95% confidence interval (CI). Leave-one-out analysis, heterogeneity tests, and horizontal pleiotropy tests were performed to validate the stability and reliability of the results.
Results
The IVW for liver iron content was OR = 1.0009, 95% CI: 1.0005–1.0013, using 10 single nucleotide polymorphisms (SNPs) as instrumental variables. The IVW for percent liver fat with 10 SNPs as instrumental variables was OR = 1.0015, 95% CI: 1.0012–1.0019. The IVW for liver volume was OR = 1.0009, 95% CI: 0.9996–1.0023, using 11 SNPs as instrumental variables. The IVW p-values for liver iron content and percent liver fat were less than 0.05, indicating a causal relationship with HCC. However, the IVW p-value for liver volume was greater than 0.05, suggesting no causal relationship between liver volume and HCC.
Conclusion
This study revealed that higher liver iron content and percent liver fat were associated with an increased risk of hepatocellular carcinoma. However, no causal relationship was established between liver volume and HCC.
Effectiveness of prismatic combination treatment on children with amblyopia combined with esotropia
Authors: Xiao man-yi , Wu Yin , Qi Xin , Wei Xin , Hu Ye 
Published: November 2023

Abstract
Purpose
Prism has been used as a non-surgical treatment for certain types of strabismus; however, its role as the primary intervention for amblyopia combined with esotropia has been rarely studied. The objective of this study was to compare the effectiveness of prismatic correction with traditional occlusion therapy in treating amblyopia combined with esotropia.
Methods
This retrospective case-control study included children aged 3 to 16 years with amblyopia combined with esotropia. After refractive correction, the patients were divided into two groups. The control group received traditional occlusion therapy combined with binocular treatment, while the prism group received full correct prisms combined with binocular treatment. Clinical characteristics, visual acuity, binocular functions, and the angle of esodeviation were assessed at each follow-up examination. The treatment outcomes after 12 months were evaluated to determine the more effective protocol.
Results
At baseline, there were no significant differences in age, gender ratio, best-corrected visual acuity (BCVA) of the amblyopic eye, deviation amount (△), and binocular vision function between the two groups (all P > 0.05). After treatment, both groups showed improvement in amblyopic eye visual acuity. The difference in visual acuity improvement between the two groups was not statistically significant at 6 months (0.18±0.17 vs 0.14±0.11, P > 0.05), but it was statistically significant at 12 months (0.35±0.20 vs 0.25±0.18, P < 0.05). The prism group demonstrated a significant improvement in binocular vision function, whereas there was no significant change in the control group at 6 months or 12 months after treatment. The deviation amount (△) remained stable in both groups, with no significant difference between the two groups after treatment.
Conclusion
Prismatic treatment combined with binocular treatment was more effective in improving amblyopic eye visual acuity and binocular vision compared to traditional occlusion therapy combined with binocular treatment in children with amblyopia combined with esotropia. Prismatic correction could be considered as the first-line treatment for amblyopia combined with esotropia.