bluuebunny/crossref_metadata_2025_split huggingface dataset:
ALOX5 acts as a key role in regulating the immune microenvironment in intrahepatic cholangiocarcinoma, recruiting tumor-associated macrophages through PI3K pathway
Authors: Chen Jialu , Tang Yue , Qin Delong , Yu Xiaopeng , Tong Huanjun , Tang Chengwei , Tang Zhaohui 
Published: December 2023

Abstract
Background
Intrahepatic cholangiocarcinoma (ICC) is poorly treated due to the presence of an inhibitory immune microenvironment. Tumor-associated macrophages (TAM) are an important component of TME. ALOX5 is an important lipid metabolism enzyme in cancer progression, but the mechanism by which it regulates TAM to promote ICC progression is unknown. The aim of this study was to investigate the potential mechanism of TAM regulation by ALOX5 and the translational effect of targeting ALOX5.

Methods
In this study, we investigated the association between the spatial localization of epithelial cells and TAMs by combining scRNA-seq analysis with multiplex immunofluorescence analysis. Through bulk sequencing analysis and spatial analysis, lipid metabolism genes closely related to TAM infiltration were screened. In vitro co-culture model was constructed to verify that ALOX5 and its downstream metabolite LTB4 promote M2 macrophage migration. Bulk sequencing after co-culture combined with single-cell analysis was performed to identify key pathways for up-regulation of M2 macrophage migration. Finally, the effect of CSF1R inhibitor (PLX3397) combined with ALOX5 inhibitor (Zileuton) in vivo was investigated by by xenograft tumor formation experiment in nude mice.

Results
ALOX5 in ICC cells was a key lipid metabolism gene affecting the infiltration of M2 macrophages in TME. Mechanically, LTB4, a metabolite downstream of ALOX5, recruited M2 macrophages to migrate around tumor cells by binding to BLT1/BLT2 and activating the PI3K pathway, which ultimately lead to the promotion of ICC progression. Targeting CSF1R in combination with ALOX5 inhibitor effectively reduced tumor volume and M2 macrophage infiltration abundance.

Conclusion
In ICC, LTB4, a metabolite secreted by ALOX5 of epithelial cells, binded to BLT1/BLT2 on TAM surface to activate PI3K pathway and promote TAM migration, thus promoting ICC progression. Targeting CSF1R in combination with ALOX5 inhibitor for ICC is a promising combination therapy modality.
NOD2 attenuates osteoarthritis via reprogramming the activation of synovial macrophages
Authors: Li Changchuan , Ouyang Zhuji , Huang Yuhsi , Lin Sipeng , Li Shixun , Xu Jing , Liu Taihe , Wu Jionglin , Guo Peidong , Chen Zhong , Wu Haoyu , Ding Yue 
Published: December 2023

Abstract
Objective
Synovial inflammation, which precedes other pathological changes in osteoarthritis (OA), is primarily initiated by activation and M1 polarization of macrophages. While macrophages play a pivotal role in the inflammatory process of OA, the mechanisms underlying their activation and polarization remain incompletely elucidated. This study aims to investigate the role of NOD2 as a reciprocal modulator of HMGB1/TLR4 signaling in macrophage activation and polarization during OA pathogenesis.

Design
We examined NOD2 expression in the synovium and determined the impact of NOD2 on macrophage activation and polarization by knockdown and overexpression models in vitro. Paracrine effect of macrophages on fibroblast-like synoviocytes (FLS) and chondrocytes was evaluated under conditions of NOD2 overexpression. Additionally, the in vivo effect of NOD2 was assessed using collagenase VII induced OA model in mice.

Results
Expression of NOD2 was elevated in osteoarthritic synovium. In vitro experiments demonstrated that NOD2 serves as a negative regulator of HMGB1/TLR4 signaling pathway. Furthermore, NOD2 overexpression hampered the inflammatory paracrine effect of macrophages on FLS and chondrocytes. In vivo experiments revealed that NOD2 overexpression mitigated OA in mice.

Conclusions
Supported by convincing evidence on the inhibitory role of NOD2 in modulating the activation and M1 polarization of synovial macrophages, this study provided novel insights into the involvement of innate immunity in OA pathogenesis and highlighted NOD2 as a potential target for the prevention and treatment of OA.
Genome-wide identification and spatiotemporal expression analysis of cadherin superfamily members in echinoderms
Authors: Chess Macie M. , Douglas William , Saunders Josiah , Ettensohn Charles A. 
Published: December 2023

Abstract
Background
Cadherins are calcium-dependent transmembrane cell–cell adhesion proteins that are essential for metazoan development. They consist of three subfamilies: classical cadherins, which bind catenin, protocadherins, which contain 6–7 calcium-binding repeat domains, and atypical cadherins. Their functions include forming adherens junctions, establishing planar cell polarity (PCP), and regulating cell shape, proliferation, and migration. Because they are basal deuterostomes, echinoderms provide important insights into bilaterian evolution, but their only well-characterized cadherin is G-cadherin, a classical cadherin that is expressed by many embryonic epithelia. We aimed to better characterize echinoderm cadherins by conducting phylogenetic analyses and examining the spatiotemporal expression patterns of cadherin-encoding genes during Strongylocentrotus purpuratus development.

Results
Our phylogenetic analyses conducted on two echinoid, three asteroid, and one crinoid species identified ten echinoderm cadherins, including one deuterostome-specific ortholog, cadherin-23, and an echinoderm-specific atypical cadherin that possibly arose in an echinoid-asteroid ancestor. Catenin-binding domains in dachsous-2 orthologs were found to be a deuterostome-specific innovation that was selectively lost in mouse, while those in Fat4 orthologs appeared to be Ambulacraria-specific and were selectively lost in non-crinoid echinoderms. The identified suite of echinoderm cadherins lacks vertebrate-specific innovations but contains two proteins that are present in protostomes and absent from mouse. The spatiotemporal expression patterns of four embryonically expressed cadherins (fat atypical cadherins 1 and 4, dachsous-2, and protocadherin-9) were dynamic and mirrored the expression pattern of Frizzled 5/8, a non-canonical Wnt PCP pathway receptor protein essential for archenteron morphogenesis.

Conclusions
The echinoderm cadherin toolkit is more similar to that of an ancient bilaterian predating protostomes and deuterostomes than it is to the suite of cadherins found in extant vertebrates. However, it also appears that deuterostomes underwent several cadherin-related innovations. Based on their similar spatiotemporal expression patterns and orthologous relationships to PCP-related and tumor-suppressing proteins, we hypothesize that sea urchin cadherins may play a role in regulating the shape and growth of embryonic epithelia and organs. Future experiments will examine cadherin expression in non-echinoid echinoderms and explore the functions of cadherins during echinoderm development.
Comments on: “Mesenchymal stem cells transplantation for perianal fistulas: a systematic review and meta-analysis of clinical trials”
Authors: Cheng Fang 
Published: December 2023

AbstractThe meta-analysis by Wang et al. (Stem Cell Res Ther 14(1):103, 2023) aims to explore whether mesenchymal stem cells are effective for perianal fistulas. The authors indicated that the difference in cell types, cell sources and cell dosages did not influence mesenchymal stem cells’ efficacy, which may not be accurate. I think that local treatment with higher dosages of mesenchymal stem cells seems to not result in a higher healing rate. And, future trials should focus on donor characteristics considering past medical history of further autoimmunity, timely and cost-effective treatment to lighten the optimized therapeutic goals. In the future, it will be interesting to assess the safety and feasibility of injection of fibrin glue combined with mesenchymal stem cells in perianal fistulas.
The crucial regulatory role of type I interferon in inflammatory diseases
Authors: Ji Ling , Li Tianle , Chen Huimin , Yang Yanqi , Lu Eryi , Liu Jieying , Qiao Wei , Chen Hui 
Published: December 2023

AbstractType I interferon (IFN-I) plays crucial roles in the regulation of inflammation and it is associated with various inflammatory diseases including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and periodontitis, impacting people's health and quality of life. It is well-established that IFN-Is affect immune responses and inflammatory factors by regulating some signaling. However, currently, there is no comprehensive overview of the crucial regulatory role of IFN-I in distinctive pathways as well as associated inflammatory diseases. This review aims to provide a narrative of the involvement of IFN-I in different signaling pathways, mainly mediating the related key factors with specific targets in the pathways and signaling cascades to influence the progression of inflammatory diseases. As such, we suggested that IFN-Is induce inflammatory regulation through the stimulation of certain factors in signaling pathways, which displays possible efficient treatment methods and provides a reference for the precise control of inflammatory diseases.
The phytolith carbon sequestration in terrestrial ecosystems: the underestimated potential of bamboo forest
Authors: Cheng Xuekun , Lv Huiru , Liu Shuhan , Li Chong , Li Pingheng , Zhou Yufeng , Shi Yongjun , Zhou Guomo 
Published: December 2023

Abstract
Background
Terrestrial ecosystems contain significant carbon storage, vital to the global carbon cycle and climate change. Alterations in human production activities and environmental factors affect the stability of carbon storage in soil. Carbon sequestration in plant phytoliths offers a sustainable method for long-term carbon stabilization. Carbon occluded in phytoliths (PhytOC) is a kind of carbon that can be stable and not decomposed for a long time, so it is crucial to conduct more in-depth research on it.

Results
We undertook a meta-analysis on PhytOC across global terrestrial ecosystems, analyzing 60 articles, encapsulating 534 observations. We observed notable differences in phytolith and PhytOC contents across various ecosystems. Bamboo forest ecosystems exhibited the highest vegetation phytolith and PhytOC content, while soil phytolith content was most prominent in bamboo forests and PhytOC content in croplands. Human activities, such as grassland grazing, had a lesser impact on soil PhytOC transport than actions like cutting and tillage in croplands and forests. Our study separated bamboo ecosystems, analyzing their PhytOC content and revealing an underestimation of their carbon sink capacity.

Conclusions
Notwithstanding our findings, phytoliths’ intricate environmental interactions warrant further exploration, crucial for refining ecosystem management and accurately estimating PhytOC stocks. This deepened understanding lays the foundation for studying phytoliths and the carbon sink dynamics.
Psychometric properties of self-report measures of eating disorder cognitions: a systematic review
Authors: Hatoum Amaani H. , Burton Amy L. , Berry Sophie L. , Abbott Maree J. 
Published: December 2023

Abstract
Background
Although eating disorder (ED) models display some differences in theory and treatment approach, cognitive-behavioural, schema-focused, and disorder-specific models all highlight the fundamental nature of cognitions as key factors in ED development and maintenance processes. As such, it is vital that ED cognitions continue to be assessed and monitored as therapeutic targets and treatment outcomes as well as being examined as constructs in empirical research. This review aimed to systematically identify and evaluate the psychometric properties of existing self-report measures of ED cognitions.

Methods
A systematic review protocol was registered using the international prospective register of systematic reviews (PROSPERO; CRD42023440840). Included studies described the development, validation and/or the psychometric evaluation of a measure (or subscale) that was specifically developed to solely assess ED cognitions (that is thoughts, expectations, assumptions, or beliefs), in English-speaking, adult populations. The search was conducted using three electronic databases: PsycINFO, MedLine, and Embase. Two independent reviewers conducted screening, selection and evaluation of the psychometric properties of relevant measures using a standardised, well-established quality appraisal tool.

Results
Of the initial search of 7581 potential studies, 59 met inclusion criteria and described the psychometric evaluation of 31 measures (or subscales) of ED cognitions. The findings from the current review indicate that of the included measures, none currently meet all nine criteria of adequate psychometric properties. The Eating Beliefs Questionnaire (EBQ; and EBQ-18), and the Eating Disorder Inventory Body Dissatisfaction subscale (EDI [BD]) currently possess the most evidence supporting their validity, reliability, and clinical utility.

Conclusions
The findings of the current systematic review provide guidance for future researchers to focus efforts on improving evidence for the validity, reliability and utility of self-report measures of ED cognitions. Overall, the present study has provided a detailed and systematic evaluation to support researchers and clinicians in future selection of measures of ED cognitions dependent on the specific aims of their research and treatment.
The quest for optimal femoral torsion angle measurements: a comparative advanced 3D study defining the femoral neck axis
Authors: Van fraeyenhove Bert , Verhaegen Jeroen C. F. , Grammens Jonas , Mestach Gino , Audenaert Emmanuel , Van Haver Annemieke , Verdonk Peter 
Published: December 2023

AbstractPurposeThere is high variability in femoral torsion, measured on two‐dimensional (2D) computed tomography (CT) scans. The aim of this study was to find a reliable three‐dimensional (3D) femoral torsion measurement method, assess the influence of CAM deformity on femoral torsion measurement, and to promote awareness for the used measurement method.Methods3D models of 102 dry femur specimens were divided into a CAM and non‐CAM group. Femoral torsion was measured by one 2D‐CT method described by Murphy et al. (method 0) and five 3D methods. The 3D methods differed in strategies to define the femoral neck axis. Method 1 is based on an elliptical least‐square fit at the middle of the femoral neck. Methods 2 and 3 defined the centre of mass of the entire femoral neck and of the most cylindrical part, respectively. Methods 4 and 5 were based on the intersection of the femoral neck with a 25% and 40% enlarged best fit sphere of the femoral head.Results3D methods resulted in higher femoral torsion measures than the 2D method; the mean torsion for method 0 was 8.12° ± 7.30°, compared to 9.93° ± 8.24° (p < 0.001), 13.21° ± 8.60° (p < 0.001), 8.21° ± 7.64° (p = 1.00), 9.53° ± 7.87° (p < 0.001) and 10.46° ± 7.83° (p < 0.001) for methods 1 to 5 respectively. In the presence of a CAM, torsion measured with method 4 is consistently smaller than measured with method 5.Conclusion2D measurement might underestimate true femoral torsion and there is a difference up to 5°. There is a tendency for a higher mean torsion in hips with a CAM deformity. Methods 4 and 5 are the most robust techniques. However, method 4 might underestimate femoral torsion if a CAM deformity is present. Since method 5 is independent of a CAM deformity, it is the preferred technique to define expected values of torsion.
Severe perioperative lactic acidosis in a pediatric patient with glycogen storage disease type Ia: a case report
Authors: Takahashi Tamayo , Oue Kana , Imado Eiji , Doi Mitsuru , Shimizu Yoshitaka , Yoshida Mitsuhiro 
Published: December 2023

Abstract
Background
Glycogen storage disease (GSD) is a group of rare inherited metabolic disorders caused by enzyme deficiencies in glycogen catabolism. GSD type Ia is a congenital deficiency of the enzyme responsible for the final step in glucose production by glycolysis, resulting in impaired carbohydrate metabolism.

Case presentation
A 14-year-old boy with GSD type Ia was scheduled for a maxillary cystectomy under general anesthesia. He was taking oral sugars such as uncooked cornstarch regularly to prevent hypoglycemia. Perioperatively, glucose was administered via the peripheral vein for fasting; however, severe lactic acidosis occurred. He also developed hypercapnia because of intraoperative poor ventilation caused by hepatomegaly.

Conclusions
We experienced a child with GSD type Ia who developed severe lactic acidosis despite continuous glucose infusion. Further studies are required to determine appropriate perioperative management for patients with GSD, including fasting glucose administration.
Urachal mucinous cystic tumor of low malignant potential: a report of a rare case with literature review
Authors: George Moyo Mthabisi Talent , Dirilenoğlu Fikret , Köy Yazgı 
Published: December 2023

AbstractNeoplasms originating from the urachus are exceptionally uncommon, comprising only 0.17% of all bladder neoplasms. Among these, the mucinous cystic tumor of low malignant potential (MCTLMP) is particularly rare, with only 25 documented cases in medical literature. Despite their rarity, it is essential to identify MCTLMPs given the possibility of severe complications. Fortunately, surgical removal offers promising cure rates. In this report, A 40-year-old female patient presented to the hospital with abdominal pain. Subsequently, a CT scan showed a 9-cm cystic mass pressing the bladder. The lesion was surgically excised and histopathological examination was performed. Grossly, the specimen consisted of a cyst with a smooth external surface and mucinous content. Microscopically, the cyst was lined by columnar mucinous epithelial cells that included basally-located hyperchromatic nuclei and scattered Goblet cells consistent with an intestinal phenotype. The epithelial cells show mild atypia with focal papillary formations and flattening due to compression. Immunohistochemically, the epithelial tumor cells expressed cytokeratin 20, CDX2, beta-catenin (membranous); and were negative for cytokeratin 7. The location, histopathological, and immunohistochemical findings were consistent with a diagnosis of MCTLMP. In this report, we present an instance of urachal MCTLMP, which represents less than 30 cases documented in the medical literature. This case study marks the importance of early identification of MCTLMP due to their potential for severe complications, despite the tumor’s low malignant potential and to exclude more aggressive tumors with areas of intraepithelial or invasive carcinoma.
Ecosystem services valuation for supporting sustainable life below water
Authors: Koundouri Phoebe , Halkos George , Landis Conrad Felix Michel , Alamanos Angelos 
Published: December 2023

AbstractThe significance of the SDGs lies in their holistic, global and interdisciplinary nature. But this nature at the same time poses significant challenges, as it is difficult to bridge the breadth of different aspects included in the SDGs, such as the environmental and the socio-economic, both in theory, practical application and policymaking. SDG14 on “life below water” is quite a holistic concept as it refers to a natural/environmental system (seas), supporting several marine economic activities and ecosystem values, and associated with strong social and cultural characteristics of the local populations, affecting the ways they manage marine areas. The main challenges for the achievement of a sustainable life below water are analyzed, and ways forward are discussed. Holistic and well-coordinated approaches considering the complex nature of SDG14 are necessary. Moreover, we argue on the role of economic instruments that can bridge environmental and socio-economic aspects, towards more sustainable life below water. In particular, the potential of environmental valuation as a means to better inform SDG policies, is discussed, using the example of SDG14. The currently established frameworks for Country’s Sustainability Reporting, lack metrics focusing on the economic impact of the environment and the ecosystem services’ degradation or restoration rates, including ocean and marine ecosystems. Acknowledging and quantifying the costs and benefits of ocean and marine ecosystems can lead to more effective interventions (such as ocean pollution prevention, climate change mitigation, fishing exploitation, biodiversity and coral reef preservation) and a better understanding of human-environmental dynamics. This, in turn, strengthens coordinated management and cooperation.
Correlation analysis between polymorphism of leptin and IGFI genes and body measurements in Barki and Farafra sheep
Authors: Darwish Ahmed M. , Abdelhafez Mohamed A. , Abdel-Hamid Zeinab G. , Othman Sarah I. , Mohamed Ibrahim E. , Allam Ahmed A. 
Published: December 2023

Abstract
Background
Growth performance, which can be evaluated using various body measurements, is crucial for providing red meat for human consumption. This study aims to improve the growth performance of sheep by identifying the different genotypes of leptin and IGFI genes associated with higher body measurements.

Methods
In this study, we collected blood samples from Barki (n = 30) and Farafra (n = 30) sheep, and measured their body height, width, length, and weight, and tail width and length to assess their growth performance. We used single strand conformation polymorphism polymerase chain reaction (SSCP-PCR) and sequencing analysis to identify single nucleotide polymorphisms (SNPs) and different genotypes of the leptin and insulin-like growth factor receptor (IGFI) genes associated with sheep body measurements.

Results
The leptin gene (209 bp) and IGFR gene (345 bp) were found to have three (TT, TG, and GG) and (TT, TC, and CC) genotypes, respectively, by SSCP-PCR. Sequence analysis revealed a SNP in the leptin gene (200G > T) with two alleles (G allele: OR058867 and T allele: OR058868). This SNP resulted in the substitution of the amino acid valine with leucine, which increased the width, length, and weight of the body and tail width in the heterozygous form (TG). The IGFI gene had three SNPs: 305 G > T in TT genotype (OR260670), 184C > T, 265T > C, and 305G > T in TC genotype (OR260669), and 265T > C in CC genotype (OR260671). The TC genotype of the IGFR gene was correlated with higher values of body measurements. Barki sheep were found to have the highest values for body weight, width, length, and tail width compared to Farafra sheep.

Conclusion
We recommend the use of entire Barki sheep in inbreeding programs to improve growth performance in Egyptian sheep.