bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation der Gewaltopferambulanz des Instituts für Rechtsmedizin Greifswald durch die Betroffenen
Authors: Wudtke J. , Bockholdt B. , Fokuhl A. , Stobbe F. , Dokter M. 
Published: January 2024

ZusammenfassungNach über 12 Jahren Untersuchungserfahrung in der Gewaltopferambulanz des Instituts für Rechtsmedizin Greifswald entstand das Bedürfnis nach einer Bewertung des aus ärztlicher Sicht etablierten Untersuchungssettings durch die Betroffenen. Es wurden in einer freiwilligen, anonymisierten Studie die von den Betroffenen subjektiv wahrgenommenen Untersuchungsbedingungen anhand eines dafür entwickelten Fragebogens kategorisiert erfragt. Erfasst wurden neben den persönlichen Angaben (Lebensalter/Geschlecht) Analysekriterien wie die Gestaltung der Terminvergabe, die Wahl des Untersuchungsortes und die im Rahmen der Untersuchung empfundene fachliche und kommunikative Kompetenz des Untersuchers. Eingeschlossen wurden alle Betroffenen von Gewalt, die sich im Zeitraum vom 14.07.2021 bis 13.07.2022 vorstellten (n = 108). Die Bereitschaft hinsichtlich einer freiwilligen Teilnahme (onlinebasiert oder per Papierfragebogen) wurde erfragt. An der Studie nahmen 23 Personen (13 w., 9 m., 1 n. b.) teil, was einer Rücklaufquote von 21 % entspricht. Die Probanden befanden sich in 36 % im Alter von 46 bis 55 Jahren, ein weiterer Altersschwerpunkt lag mit 27 % im Bereich von 36 bis 45 Jahren. In der überwiegenden Anzahl der Fälle (69 %) wurde der Termin selbst vereinbart. Die Terminvereinbarung wurde von 77 % der Probanden als einfach eingestuft. Als Untersuchungsort wurde in 73 % der Fälle das Institut für Rechtsmedizin Greifswald angegeben. Bei außerhalb des Instituts stattgefundenen Untersuchungen gaben 36 % der Probanden an, dass keine Möglichkeit bestanden hätte, nach Greifswald zu kommen. Die rechtsmedizinische Untersuchung und auch das Einfühlungsvermögen des Untersuchers wurden in über 70 % der Fälle als ausgezeichnet bewertet. Eine erneute Vorstellung in der Opferambulanz wurde von 80 % der Probanden mit „eindeutig ja“ beantwortet. Die anhand der Studie erhobenen Daten zeigen, dass die rechtsmedizinische körperliche Untersuchung von den untersuchten Personen als ein wertvolles Element empfunden wurde. Die Opferambulanz stellt ein gutes Konzept dar, welches durch seine flexible Gestaltung der Terminvergabe und der Wahl des Untersuchungsortes den Bedürfnissen eines Flächenlandes und auch den Wünschen der Betroffenen gerecht wird.
Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes
Authors: Vojnovic Sandra , Aleksic Ivana , Ilic-Tomic Tatjana , Stevanovic Milena , Nikodinovic-Runic Jasmina 
Published: January 2024

Abstract
The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.

Key points
• Bacillus and Streptomyces spp. as robust hosts for enzyme production.
• Industrially relevant enzyme groups for production in alternative hosts highlighted.
• Molecular biology techniques are enabling easier utilization of both hosts.
Findings in magnetic resonance imaging for restaging locally advanced rectal cancer
Authors: Marjasuo Suvi , Koskenvuo Laura , Lepistö Anna 
Published: January 2024

Abstract
Purpose
We aimed to assess the prognostic value of restaging magnetic resonance imaging (MRI) in rectal cancer after neoadjuvant therapy and compare long-course chemoradiotherapy (LC-CRT) to short-course radiotherapy with delayed surgery (SCRT-delay).

Methods
This retrospective study included 267 patients with locally advanced rectal cancer (LARC) operated on between January 2016 and April 2019, all of whom received either LC-CRT or SCRT-delay in the neoadjuvant setting. The primary outcomes were overall survival (OS) and cancer-specific survival (CSS) based on radiological response assessed using the magnetic resonance tumor regression grade (mrTRG).

Results
In the LC-CRT group, cumulative 1-, 3-, and 5-year OS rates were 94.8%, 86.4%, and 79.0%, while in the SCRT-delay group, they were 83.3%, 68.9%, and 68.9% (P = 0.017). For CSS in the LC-CRT group, cumulative rates were 96.9%, 90.3%, and 85.0%, and in the SCRT-delay group, they were 88.6%, 81.4%, and 81.4% (P = 0.222). There were no significant differences in total histological response rates or local recurrence rates between the treatment groups. The good and moderate response group (mrTRG 1–3) had significantly better cumulative 1-, 3-, and 5-year OS and CSS compared to the poorer response group (mrTRG 4–5) (P = 0.023 for OS and P = 0.048 for CSS).

Conclusion
Unfavorable MRI response is a sign of poor prognosis in LARC. SCRT-delay is comparable to LC-CRT concerning the oncological outcome.
Ocrelizumab reduces cortical and deep grey matter loss compared to the S1P-receptor modulator in multiple sclerosis
Authors: Bajrami Albulena , Tamanti Agnese , Peloso Angela , Ziccardi Stefano , Guandalini Maddalena , Calderone Milena , Castellaro Marco , Pizzini Francesca B. , Montemezzi Stefania , Marastoni Damiano , Calabrese Massimiliano 
Published: January 2024

Abstract
Introduction
Ocrelizumab (OCR) and Fingolimod (FGL) are two high-efficacy treatments in multiple sclerosis which, besides their strong anti-inflammatory activity, may limit neurodegeneration.

Aim
To compare the effect of OCR and FGL on clinical and MRI endpoints.

Methods
95 relapsing–remitting patients (57 OCR, 38 FGL) clinically followed for 36 months underwent a 3-Tesla MRI at baseline and after 24 months. The annualized relapse rate, EDSS, new cortical/white matter lesions and regional cortical and deep grey matter volume loss were evaluated.

Results
OCR reduced the relapse rate from 0.48 to 0.04, FGL from 0.32 to 0.05 (both p < 0.001). Compared to FGL, OCR-group experienced fewer new white matter lesions (12% vs 32%, p = 0.005), no differences in new cortical lesions, lower deep grey matter volume loss (− 0.12% vs − 0.66%; p = 0.002, Cohen’s d = 0.54), lower global cortical thickness change (− 0.45% vs − 0.70%; p = 0.036; d = 0.42) and reduced cortical thinning/volume loss in several regions of interests, including those of parietal gyrus (d-range = 0.65–0.71), frontal gyrus (d-range = 0.47–0.60), cingulate (d-range = 0.41–0.72), insula (d = 0.36), cerebellum (cortex d = 0.72, white matter d = 0.44), putamen (d = 0.35) and thalamus (d = 0.31). The effect on some regional thickness changes was confirmed in patients without focal lesions.

Conclusions
When compared with FGL, patients receiving OCR showed greater suppression of focal MRI lesions accumulation and lower cortical and deep grey matter volume loss.
Association between iron deficiency anemia and subsequent stomach and colorectal cancer diagnosis in Germany
Authors: Krieg Sarah , Loosen Sven , Krieg Andreas , Luedde Tom , Roderburg Christoph , Kostev Karel 
Published: January 2024

Abstract
Purpose
Iron deficiency anemia (IDA) is the most common form of anemia worldwide, resulting in a high burden of disease. Accumulating evidence suggests that IDA is associated with the development of gastrointestinal (GI) cancers.

Methods
Data from the IDA database (IQVIA) of primary care practices in Germany of adult patients first diagnosed with IDA between January 2005 and December 2021 were retrospectively analyzed and compared with a 1:1 propensity score-adjusted cohort without IDA. Study outcomes were first stomach cancer or colorectal cancer (CRC) diagnosis up to 10 years after the index date as a function of IDA.

Results
A total of 122,502 individuals with IDA and 122,502 individuals without IDA were included. The 10-year cumulative incidence of CRC was 1.4% in the IDA patients compared to 0.8% in the cohort without IDA (p < 0.001). Regression analysis revealed a significant association between IDA and subsequent CRC (HR 2.05; 95% CI 1.83–2.30). Stomach cancer was diagnosed in 0.3% of IDA patients compared to 0.2% in the non-IDA cohort during the 10-year follow-up period (p = 0.002). However, this was significant only in the age group > 80 years (HR 2.73; 95% CI 1.60–4.67) and in men (HR 1.90; 95% CI 1.38–2.61).

Conclusion
These findings add to the literature and suggest an association between IDA and GI cancers. The extent to which this association is due to GI bleeding or other pathophysiological processes that may be caused by IDA requires further investigation, particularly experimental studies.
Vitamin D-mediated tsRNA-07804 triggers mitochondrial dysfunction and suppresses non-small cell lung cancer progression by targeting CRKL
Authors: Liang Yonggang , Zhang Xiaoqiang , Peng Jinhua , Liu Jing , Chen He , Guo Shanxian 
Published: January 2024

Abstract
Objective
tRNA-derived small RNAs (tsRNAs) are novel non-coding RNAs with various functions in multiple cancers. Nevertheless, whether vitamin D executes its function in mitochondrial dysfunction and non-small cell lung cancer (NSCLC) progression through tsRNAs remains obscure.

Methods
Differentially expressed tsRNAs between control and vitamin D-treated H1299 cells were acquired by small RNA sequencing. Cell and animal experiments were implemented to elucidate the impacts of vitamin D and tsRNA on mitochondrial dysfunction and NSCLC progression. Dual-luciferase reporter assay, quantitative real-time PCR, western blot and recovery experiments were applied to determine the mechanism of tsRNA in NSCLC.

Results
We discovered that vitamin D receptor resulted in decreased mitochondrial-related functions and vitamin D caused mitochondrial dysfunction of NSCLC cells. tsRNA-07804 was remarkably upregulated in vitamin D-treated H1299 cells. Functional experiments indicated that vitamin D led to mitochondrial dysfunction, repressed the proliferation, migration, invasion, and promoted apoptosis of H1299 cells via regulating tsRNA-07804. Mechanistically, tsRNA-07804 induced mitochondrial dysfunction and inhibited the malignancy of H1299 cells by suppressing CRKL expression. In vivo experiments showed that vitamin D inhibited the tumor growth in NSCLC by increasing tsRNA-07804 expression. Moreover, clinical sample analysis unveiled that tsRNA-07804 had a negative correlation with CRKL.

Conclusions
In conclusion, our study proved that vitamin D induced mitochondrial dysfunction and suppressed the progression of NSCLC through the tsRNA-07804/CRKL axis. Overall, these results unveiled that tsRNA-07804 might act as a potential therapeutic target for NSCLC.
Linear and bilinear Fourier multipliers on Orlicz modulation spaces
Authors: Blasco Oscar , Öztop Serap , Üster Rüya 
Published: January 2024

AbstractLet $$\Phi _i, \Psi _i$$

Φ
i

,

Ψ
i

be Young functions, $$\omega _i$$

ω
i

be weights and $$M^{\Phi _i,\Psi _i}_{\omega _i}(\mathbb {R} ^{d})$$

M

ω
i

Φ
i

,

Ψ
i

(

R

d

)

be the corresponding Orlicz modulation spaces for $$i=1,2,3$$

i
=
1
,
2
,
3

. We consider linear (respect. bilinear) multipliers on $$\mathbb {R} ^{d}$$

R

d

, that is bounded measurable functions $$m(\xi )$$

m
(
ξ
)

(respect. $$m(\xi ,\eta )$$

m
(
ξ
,
η
)

) on $$\mathbb {R} ^{d}$$

R

d

(respect. $$\mathbb {R} ^{2d}$$

R

2
d

) such that $$\begin{aligned} T_m(f)(x)=\int _{\mathbb {R} ^{d}}{\hat{f}}(\xi ) m(\xi )e^{2\pi i \langle \xi , x\rangle }d\xi \end{aligned}$$

T
m

(
f
)

(
x
)

=

∫

R

d

f
^

(
ξ
)

m

(
ξ
)

e

2
π
i
⟨
ξ
,
x
⟩

d
ξ

(respect. $$\begin{aligned} B_m(f_1,f_2)(x)=\int _{\mathbb {R} ^{d}}\int _{\mathbb {R} ^{d}} \hat{f_1}(\xi ) \hat{f_2}(\eta )m(\xi ,\eta )e^{2\pi i \langle \xi +\eta , x\rangle }d\xi d\eta \end{aligned}$$

B
m

(

f
1

,

f
2

)

(
x
)

=

∫

R

d

∫

R

d

f
1

^

(
ξ
)

f
2

^

(
η
)

m

(
ξ
,
η
)

e

2
π
i
⟨
ξ
+
η
,
x
⟩

d
ξ
d
η

define a bounded linear (respect. bilinear) operator from $$M^{\Phi _1,\Psi _1}_{\omega _1}(\mathbb {R} ^{d})$$

M

ω
1

Φ
1

,

Ψ
1

(

R

d

)

to $$M^{\Phi _2,\Psi _2}_{\omega _2}(\mathbb {R} ^{d})$$

M

ω
2

Φ
2

,

Ψ
2

(

R

d

)

(respect. $$M^{\Phi _1,\Psi _1}_{\omega _1}(\mathbb {R} ^{d})\times M^{\Phi _2,\Psi _2}_{\omega _2}(\mathbb {R} ^{d})$$

M

ω
1

Φ
1

,

Ψ
1

(

R

d

)

×

M

ω
2

Φ
2

,

Ψ
2

(

R

d

)

to $$M^{\Phi _3,\Psi _3}_{\omega _3}(\mathbb {R} ^{d})$$

M

ω
3

Φ
3

,

Ψ
3

(

R

d

)

). In this paper we study some properties of these spaces and give methods to generate linear and bilinear multipliers between Orlicz modulation spaces.
Trans-lateral ventricular approach for surgical treatment of high-located P2–P3 junction posterior cerebral artery aneurysms: from anatomical research to clinical application
Authors: Muhammad Sajjad , Zhang Rui , Filler Timm , Hänggi Daniel , Meling Torstein R. 
Published: January 2024

Abstract

Background
Posterior cerebral artery (PCA) aneurysms, though rare, pose treatment challenges. Endovascular therapy is the preferred option, but microsurgery becomes necessary in certain cases. Various microsurgical approaches have been suggested for PCA aneurysms, particularly those at the P2–P3 junction. This study highlights the trans-lateral ventricular approach (TVA) for addressing these complex aneurysms. This study aims to assess the feasibility and safety of the trans-lateral ventricular approach (TVA) for treating high-located complex PCA aneurysms at the P2–P3 junction. The study evaluates both clinical outcomes and anatomical considerations.

Methods
Two cases of PCA aneurysms at the P2–P3 junction were treated using TVA in 2019. Navigation-guided entry via the interparietal sulcus was planned. Ventriculostomy was performed from the cortex to the lateral ventricle’s atrium. Medial atrial floor dissection exposed PCA’s P2–P3 segments. Neuronavigation and ultrasound-aided guidance was used. Anatomical studies on fixed and contrast-perfused specimens refined the approach.

Results
Both cases saw successful aneurysm clipping. The unruptured aneurysm patient was discharged in 6 days. The poor-grade SAH patient required extended ICU care, moving to rehabilitation with mRS = 4. The unruptured complex aneurysm case exhibited no deficits, returning to work in 3 months. Anatomical dissections validated TVA for high-located P2–P3 junction PCA aneurysms.

Conclusion
While endovascular therapy remains primary, this study demonstrates the viability of navigation-guided TVA for select high-located P2–P3 junction PCA aneurysms. Successes and challenges underscore the importance of patient selection and anatomical awareness.
Obsessive–compulsive symptoms and brain lesions compatible with multiple sclerosis
Authors: von Zedtwitz Katharina , Tebartz van Elst Ludger , Urbach Horst , Groppa Sergiu , Schiele Miriam A. , Prüss Harald , Domschke Katharina , Stich Oliver , Hannibal Luciana , Endres Dominique 
Published: January 2024

AbstractAutoimmune-mediated obsessive–compulsive disorder (OCD) can occur in multiple sclerosis (MS). Here, a well-studied case study of a patient with OCD and MS-compatible diagnostic findings is presented. The 42-year-old female patient had displayed OCD symptoms for 6 years. Magnetic resonance imaging (MRI) identified several periventricular and one brainstem lesion suggestive of demyelination. Cerebrospinal fluid (CSF) analyses detected an increased white blood cell count, intrathecal immunoglobulin (Ig) G and IgM synthesis, CSF-specific oligoclonal bands, and a positive MRZ reaction. Neopterin was increased, but sarcoidosis was excluded. In the absence of neurological attacks and clues for MRI-based dissemination in time, a radiologically isolated syndrome, the pre-disease stage of MS, was diagnosed. Neurotransmitter measurements of CSF detected reduced serotonin levels. In the absence of visible strategic demyelinating lesions within the cortico-striato-thalamo-cortical circuits, OCD symptoms may relate to reduced intrathecal serotonin levels and mild neuroinflammatory processes. Serotonin abnormalities in MS should be studied further, as they could potentially explain the association between neuroinflammation and mental illnesses.
IL-39 and IL-35 gingival crevicular fluid levels in diabetic patients with generalized periodontitis
Authors: Hassan Sandy shabaan , Abdelkawy Maha , Shaker Olfat Gamil , Tarrad Nayroz Abdel Fattah 
Published: January 2024

Abstract
Objectives
This research was performed to investigate if there is a role for IL-39 in immunopathogenesis of both systemically healthy and diabetic periodontitis patients. Additionally, to explore if we can consider IL-39 and IL-35 as biomarkers for periodontitis activity.

Materials and methods
A total of 38 periodontitis patients and 19 control volunteers were included in our study. The periodontitis patients were divided equally into (Group I), 19 patients with stage III grade C periodontitis with diabetes mellitus and (Group II), 19 patients with stage III grade B periodontitis and systemically healthy. Gingival crevicular fluid levels of each interleukin were measured pre- and postoperatively for all periodontitis patients as well as control subjects using ELISA.

Results
Our study results showed that the highest level for IL-39 was in diabetic periodontitis patients that decreased significantly postoperatively. However, the highest level for IL-35 was revealed in control group while the lowest value was registered in diabetic periodontitis patients and statistically increased after periodontal treatment.

Conclusions
Based on the results of our research, both investigated biomarkers may have a potent role in pathogenesis of periodontitis.

Clinical relevance
We could consider both interleukins as accurate diagnostic markers for periodontitis patients, regardless of diabetes mellitus association, as well as promising markers that can aid in the prevention and treatment of periodontitis patients worldwide.
Impact of initial lip competence on the outcome of class II functional appliances therapy
Authors: Sambale Janine , Jablonski-Momeni Anahita , Korbmacher-Steiner Heike Maria 
Published: January 2024

Abstract
Objectives
The aim of this prospective clinical study was to evaluate the impact of initial lip position on class II functional appliance therapy.

Materials and methods
In total, 34 class II division 1 patients (23 females, 19 males; mean age 12.4 ± 0.9 years) that met the inclusion criteria (> ½ class II molar relationship, overjet > 6 mm, ANB > 4°, neutral or horizontal growth pattern, cervical vertebral maturation stage (CVMS) II – III, mean wear-time > 10 h/day) were consecutively divided into two groups (lip incompetence (LI); lip competence (LC)). All patients were treated with the Sander bite jumping appliance (BJA). Wear time was microelectronically measured. Lateral cephalograms were taken at the beginning (T0) and after 1 year of treatment (T1). An untreated class II group served as a control (CG). Inter-group comparisons were determined with Mann–Whitney U tests for independent samples.

Results
Significant skeletal treatment effects were found in both treated groups when compared to the CG with significantly more pronounced mandibular skeletal effects in the LI than in the LC group (mandibular base length p < 0.001, composite mandibular base length p < 0.001, condylar head growth p = 0.002, co-pg p < 0.00, go-pg p = 0.003, reduction of the ANB angle p = 0.009, and Wits appraisal p < 0.001).

Conclusion
The more pronounced mandibular effects in the LI group were composed of the functional orthopedic effect plus harmonization of the lip competence.

Clinical relevance
Functional harmonization of lip incompetence with BJA enhances mandibular growth stimulation. Lip incompetence seems to impede mandibular growth and its harmonization seems to be a preventive approach.
DWSR: an architecture optimization framework for adaptive super-resolution neural networks based on meta-heuristics
Authors: Chu Shu-Chuan , Dou Zhi-Chao , Pan Jeng-Shyang , Kong Lingping , Snášel Václav , Watada Junzo 
Published: January 2024

AbstractDespite recent advancements in super-resolution neural network optimization, a fundamental challenge remains unresolved: as the number of parameters is reduced, the network’s performance significantly deteriorates. This paper presents a novel framework called the Depthwise Separable Convolution Super-Resolution Neural Network Framework (DWSR) for optimizing super-resolution neural network architectures. The depthwise separable convolutions are introduced to reduce the number of parameters and minimize the impact on the performance of the super-resolution neural network. The proposed framework uses the RUNge Kutta optimizer (RUN) variant (MoBRUN) as the search method. MoBRUN is a multi-objective binary version of RUN, which balances multiple objectives when optimizing the neural network architecture. Experimental results on publicly available datasets indicate that the DWSR framework can reduce the number of parameters of the Residual Dense Network (RDN) model by 22.17% while suffering only a minor decrease of 0.018 in Peak Signal-to-Noise Ratio (PSNR), the framework can reduce the number of parameters of the Enhanced SRGAN (ESRGAN) model by 31.45% while losing only 0.08 PSNR. Additionally, the framework can reduce the number of parameters of the HAT model by 5.38% while losing only 0.02 PSNR.