bluuebunny/crossref_metadata_2025_split huggingface dataset:
Repurposing AZD5438 and Dabrafenib for Cisplatin-Induced AKI
Authors: Pushpan Chithra K. , Kresock Daniel F. , Ingersoll Matthew A. , Lutze Richard D. , Keirns Darby L. , Hunter William J. , Bashir Khalid , Teitz Tal 
Published: November 2023

Significance Statement
To combat both untoward effects of nephrotoxicity and ototoxicity in cisplatin-treated patients, two potential therapeutic oral anticancer drugs AZD5438 and dabrafenib, a phase-2 clinical trial protein kinase CDK2 inhibitor and an US Food and Drug Administration–approved drug BRAF inhibitor, respectively, were tested in an established mouse AKI model. Both drugs have previously been shown to protect significantly against cisplatin-induced hearing loss in mice. Each drug ameliorated cisplatin-induced increases in the serum biomarkers BUN, creatinine, and neutrophil gelatinase-associated lipocalin. Drugs also improved renal histopathology and inflammation, mitigated cell death by pyroptosis and necroptosis, and significantly enhanced overall survival of cisplatin-treated mice.

Background
Cisplatin is an effective chemotherapy agent for a wide variety of solid tumors, but its use is dose-limited by serious side effects, including AKI and hearing loss. There are no US Food and Drug Administration–approved drugs to treat both side effects. Recently, two anticancer oral drugs, AZD5438 and dabrafenib, were identified as protective against cisplatin-induced hearing loss in mice. We hypothesize that similar cell stress and death pathways are activated in kidney and inner ear cells when exposed to cisplatin and tested whether these drugs alleviate cisplatin-induced AKI.

Methods
The HK-2 cell line and adult FVB mice were used to measure the protection from cisplatin-induced cell death and AKI by these drugs. Serum markers of kidney injury, BUN, creatinine, and neutrophil gelatinase-associated lipocalin as well as histology of kidneys were analyzed. The levels of markers of kidney cell death, including necroptosis and pyroptosis, pERK, and proliferating cell nuclear antigen, were also examined by Western blotting and immunofluorescence. In addition, CDK2 knockout (KO) mice were used to confirm AZD5438 protective effect is through CDK2 inhibition.

Results
The drugs reduced cisplatin-induced cell death in the HK-2 cell line and attenuated cisplatin-induced AKI in mice. The drugs reduced serum kidney injury markers, inhibited cell death, and reduced the levels of pERK and proliferating cell nuclear antigen, all of which correlated with prolonged animal survival. CDK2 KO mice were resistant to cisplatin-induced AKI, and AZD5438 conferred no additional protection in the KO mice.

Conclusions
Cisplatin-induced damage to the inner ear and kidneys shares similar cellular beneficial responses to AZD5438 and dabrafenib, highlighting the potential therapeutic use of these agents to treat both cisplatin-mediated kidney damage and hearing loss.
Эндемики Дальнего Востока в коллекции живых растений in vitro Ботанического сада-института ДВО РАН
Authors:   Ботанический сад-институт ДВО РАН, Пьянова А.С. , Сабуцкий Ю.Е. , Бердасова К.С. , Лончакова Т.Е. 
Published: November 2023

Эндемичные виды растений являются уникальными представителями флоры определенного региона. Такие виды, как правило, мало устойчивы к различным неблагоприятным условиям окружающей среды и антропогенному воздействию. Несмотря на различные стратегии поддержания редких и эндемичных видов, на сегодняшний день проблема сохранения таких видов остаётся актуальной. Одним из инструментов могут служить технологии микроклонального размножения. Цель биотехнологических приёмов состоит не в том, чтобы заменить традиционные методы сохранения, а в том, чтобы дополнить их и усовершенствовать. Коллекция живых растений in vitro Ботанического сада-института ДВО РАН включает семь эндемичных видов Дальнего Востока, большинство из которых включены в Красные книги как государственного, так и регионального уровня. Leontopodium palibinianum Beauverd (Asteraceae) – уязвимый вид. Включён в Красную книгу Приморского края [2]. Barnardia japonica (Scilla scilloides) (Thunb.) Schult. et Schult. Fil. (Asparagaceae) – на грани исчезновения. Вид включён в Красные книги Приморского края [2] и Российской федерации [3]. Lonicera tolmatchevii Pojark. (Caprifoliaceae) – уязвимый вид. Включён в Красную книгу Сахалинской области [4] и Красную книгу Российской федерации [2]. Iris mandshurica Maxim. (Iridaceae) – рекомендован для включения в готовящиеся издания Красной книги Приморского края и Красной книги России. Iris vorobievii N.S. Pavlova (Iridaceae) – угрожаемый. Включён в Красную книгу Приморского края [2] и Красную книгу Российской федерации [2]. Oxytropis chankaensis Jurtz. (Fabaceae) – уязвимый вид. Включён в Красную книгу Приморского края [2]. Lespedeza davurica (Laxm.) Schindl. (Fabaceae) – уязвимый вид.	Включён в Красные книги Приморского края [2] и Амурской области [1]. Депонирование микроклонов в культурах in vitro позволяет не только сохранять генетическое разнообразие, но обеспечивает безопасный для природных популяций обмен растительным материалом. Исследование проведено на базе уникальной научной установки «Коллекция живых растений in vitro Ботанического сада-института ДВО РАН» (реестровый номер регистрации на сайте http://ckp-rf.ru – 347296) по теме государственного задания «Введение в культуру, изучение и сохранение генетических ресурсов хозяйственно ценных растений Восточной Азии». Регистрационный номер: 122040800086-1.
Characteristics of Monsoon Semipermanent Features in Changing Climate: An Environmental Approach
Authors: Nepal Singh  , Ghulam G. Zahid  , Mohd Suhail Husain  , Nuzhat Fatima  , Ajhar Hussain  
Published: November 2023

The Indian summer monsoon (ISM) spans four months beginning from June to September (JJAS) and produced extensive rainfall over Indian continents. The land-sea heating contrast is one of the major factors facilitating ISM. ISM is managed with the aid of several monsoon semi-permanent features (MSF) which include Pakistan heat low, cross-equatorial low-level jet over the Arabian Sea, and the tropical easterly jet over the Indian Ocean at 200 hPa, Mascarene High, and seasonal anti-cyclone over the Tibet. Any fluctuations in these SMF can strongly modulate Indian Summer Monsoon Rainfall (ISMR). Therefore, it is imperative that the structure and variability of MSF need to be examined in changing climate scenarios to assess its impact on the future monsoon. Further, ISM also experiences internal variability in terms of deficit and surplus years, therefore the impact of MSF on this variability also needs to be examined. The main objective of the study is to examine the variation of rainfall in JJAS over the Indian monsoon core region and the characteristics of SMF for two contrasting monsoon years. Based on the previous study observed that the monsoon core zone has an important role to play in the ISM so for the rainfall study take the monsoon core zone for the study purpose. The 5th generation European Centre for Medium-Range Weather Forecasts (ERA5), India Meteorological Department (IMD) rainfall datasets from 1981-2020, and Coupled Model Intercomparison Project Phase 6 (CMIP6) 2015-2021 for four different SSP scenarios. In addition, to determine the changes in these MSF and their impact on rainfall for ISM. For the calculation of rainfall percentage departure of IMD and ERA5 and CMIP6 dataset following Rajeevan et al. (2010).
Coecum mobile. Literature review and clinical observation
Authors: Isaev Y. A. , Sergeev A. N. , Morozov A. M. , Shestakov M. S. 
Published: November 2023

Relevance. Anomalies of the position and attachment of the colon in the abdominal cavity includes a large number of options. In some cases, the pathology is not limited exclusively to the mobility of the cecum, but is combined with a violation of the fixation of the ascending colon, the absence of attachment of the hepatic and splenic angles, omission of the right half of the colon or total colonoptosis.Results. There is surgical pathology associated with abnormal position and attachment of the circumferential intestine in the abdomen and a case of abnormal position dependency and an anomaly of the attachment of the right side of the circumferential intestine, depending on body type, which have certain clinical manifestations. Clinical manifestations of this pathology, methods of diagnosis and treatment with favorable results are described in the article. Also own surgical treatment methods depending on the anatomical variant of the pathology are presented in the article.Conclusion. The anatomical restoration of the correct position of the intestine in the abdominal cavity is operative. The proposed operation is performed taking into account the anomaly variant.
Differential Diagnosis of Fibrotic Hypersensitivity Pneumonitis with Its Non-Fibrotic Phenotype and Usual Interstitial Pneumonia During High-Resolution Computed Tomography
Authors: Tuyrin I. E. , Kuleshov D. A. , Samsonova M. V. , Chernyaev A. L. , Kusraeva E. V. , Trushenko N. V. , Chikina S. Yu. , Avdeev S. N. 
Published: November 2023

Background. Diagnosis of hypersensitivity pneumonitis (HP) using high-resolution computed tomography (HRCT) is not an easy task. The most difficult aspects of the disease include differential diagnosis of its fibrotic (fHP) and non-fibrotic (nfHP) phenotypes, as well as their differentiation from usual interstitial pneumonia (UIP) in idiopathic pulmonary fibrosis. The determination of fibrous changes at an early stage of development can significantly accelerate the beginning of antifibrotic therapy and improve the prognosis.Objective: to identify key HRCT signs for reliable differentiation of fHP and nfHP, to carry out differential diagnostics between fHP and UIP.Material and methods. The data of 73 patients with morphologically verified HP, in whom HRCT had been performed, were retrospectively analysed. In 21 patients, nfHP was determined, and in 52 patients fHP was identified. The comparison group consisted of 24 patients with a typical radiological UIP pattern. The analysis of the changes detected during HRCT was carried out by qualitative and semi-quantitative methods. The significance of qualitative differences in a sign manifestation was assessed by Fisher’s exact test, semi-quantitative differences were evaluated using Mann–Whitney test.Results. The results of the study allow to assume, that the presence and degree of manifestation of certain HRCT signs significantly differ between the selected groups of patients in qualitative and/or semi-quantitative terms. In cases of НP, the distribution of changes was mostly uniform and diffuse, with no clear predominance in certain lobes. In UIP, diffuse craniocaudal distribution took place, and in the axial plane, the changes were mainly subpleural in nature.Conclusion. Based on the results of the study, it can be assumed that fHP significantly differs from nfHP in such features as the presence and degree of manifestation of ground glass and honeycombing symptoms, reticular changes and traction bronchiectases. When comparing the fHP and UIP groups, the distinctive signs of fHP were centrilobular nodules, mosaic pattern, as well as diffuse axial sign distribution.
Radiological Features of Changes in the Lungs Caused by Fast- or Slow-Growing Nontuberculous Mycobacteria
Authors: Amansakhedov R. B. , Dmitrieva L. I. , Smirnova T. G. , Veshkin A. A. , Ergeshov A. E. 
Published: November 2023

Objective: to compare radiological features of pulmonary nontuberculous mycobacterioses (NTM) caused by fast- or slow-growing mycobacteria.Material and methods. Radiological features of the disease were studied in 110 patients with newly diagnosed NTM. The patients were divided into two groups: 70 (63.6%) patients with slow-growing NTM and 40 (36.3%) with fast-growing NTM. The diagnosis was based on patient’s complaints, specified case history, radiological studies, clinical laboratory studies, sputum smear studies, bronchoalveolar lavage studies, different types of bronchial biopsies, and video-assisted thoracoscopic surgery resection samples. According to the results of high-resolution computed tomography (HRCT), a comparative analysis of the degree of involvement in the pathological process of such anatomical formations as parenchyma and stroma, vessels, bronchi, pleura and intrathoracic lymph nodes was carried out.Results. The analysis of HRCT studies showed that fast-growing NTM is not characterized by gross deformation of bronchopulmonary structures. There is a more pronounced infiltrative phase with the involvement of vessels, pleura and bronchial lesions of smaller generations, the formation of broncho-bronchiolectasias and symptoms of bronchiolitis, faster clinical and radiological involution under dynamic observation. Slow-growing NTM is characterized by a more severe deformation of bronchopulmonary structures with the formation of different-sized bronchiectasis, bronchogenic cavities, a more torpid course of the inflammatory process.Conclusion. Despite the similarities of clinical and radiological patterns of NTM, we established some distinguished features for slow-growing and fast-growing types.
Forecasting SARS-CoV-2 Epidemic Dynamic in Poland with the pDyn Agent-Based Model
Authors: Niedzielewski Karol , Bartczuk Rafał P. , Bielczyk Natalia , Bogucki Dominik , Dreger Filip , Dudziuk Grzegorz , Górski Łukasz , Gruziel-Słomka Magdalena , Haman Jędrzej , Kaczorek Artur , Kisielewski Jan , Krupa Bartosz , Moszyński Antoni , Nowosielski Jędrzej M. , Radwan Maciej , Semeniuk Marcin , Tymoszuk Urszula , Zieliński Jakub , Rakowski Franciszek 
Published: November 2023

Abstract

We employ pDyn, an agent-based epidemiological model, to forecast the fourth wave of the SARS-CoV-2 epidemic, primarily driven by the Delta variant, in Polish society. The model captures spatiotemporal dynamics of the epidemic spread, predicting disease-related states based on pathogen properties and behavioral factors.
We assess pDyn's validity, encompassing pathogen variant succession, immunization level, and the proportion of vaccinated among confirmed cases. We evaluate its predictive capacity for pandemic dynamics, including wave peak timing, magnitude, and duration for confirmed cases, hospitalizations, ICU admissions, and deaths, nationally and regionally in Poland.
Validation involves comparing pDyn's estimates with real-world data (excluding data used for calibration) to evaluate whether pDyn accurately reproduced the epidemic dynamics up to the simulation time. To assess the accuracy of pDyn's predictions, we compared simulation results with real-world data acquired after the simulation date.
The findings affirm pDyn's accuracy in forecasting and enhancing our understanding of epidemic mechanisms.
Characterization of the Fusarium circinatum biofilm environmental response role
Authors: Ratsoma Francinah M , Mokoena Nthabiseng Z , Santana Quentin C , Wingfield Brenda D , Steenkamp Emma T , Motaung Thabiso E 
Published: November 2023

Abstract

The capacity to form biofilms is a common trait among many microorganisms present on Earth. In this study, we demonstrate for the first time that the fatal pine pitch canker agent, Fusarium circinatum, can lead a biofilm-like lifestyle with aggregated hyphal bundles wrapped in extracellular matrix (ECM). Our study suggests that F. circinatum biofilms respond to a changing environment, demonstrated by poor (lower metabolic activities ODs) and optimal (higher metabolic activities ODs) biofilm development under particular abiotic conditions, including temperature and pH. Further analysis revealed that while planktonic cells produced small amounts of ECM per unit of the biomass, heat- and azole-exposed biofilms produced significantly more ECM than non-exposed biofilms. The increased synthesis of ECM in biofilms due to these abiotic factors underscores biofilm importance as a means of survival in response to various stress conditions, demonstrating the adaptability of F. circinatum to changing environments. Interestingly, azole exposure also led to biofilms that were resistant to DNase, which typically uncouples biofilms by penetrating the biofilm and degrading its extracellular DNA; we propose that DNases were likely hindered from reaching target cells by the ECM barricade. The interplay between antifungal treatment and DNase enzyme suggests a complex relationship between eDNA, ECM, and antifungal agents in F. circinatum biofilms. Therefore, our results show how a phytopathogen’s sessile (biofilm) lifestyle could influence its response to the surrounding environment.
Significant benefits from international cooperation over marine plastic pollution
Authors: de Vries Frans , Beaumont Nicola , Börger Tobias , Clark James , Hanley Nicholas , Johnston Robert , Meginnis Keila , Stapenhurst Christopher 
Published: November 2023

Abstract

Plastic pollution in the world’s oceans threatens marine ecosystems and biodiversity, leading to a loss of well-being for people1,2. The connected nature of the marine environment suggests that coordinated actions by countries sharing a common ocean border may provide more effective pollution control than unilateral actions by any one country. However, economic theory and empirical evidence suggest that countries often fail to cooperate, even when joint welfare would be higher under cooperation3. Here we provide the first analysis of the potential economic benefits of cooperative marine plastic pollution (MPP) management in the North Atlantic. An estimated transfer matrix showing how plastics move across the North Atlantic is combined with game theory and estimates of benefits and costs to derive the potential net benefits of international cooperation. A fully cooperative outcome across 16 countries leads to a substantial reduction in MPP, resulting in significant aggregate annual net benefits. However, MPP reduction burdens are unevenly spread across countries. Constraining the agreement to avoid such consequences results in both less MPP reduction and lower aggregate benefits. As the United Nations works on a future global plastic pollution treaty, these results demonstrate that close cooperation will be a critical determinant of its success.
Microenvironment of Adjacent Non-Neoplastic Regions Determines Prognostic Outcomes in Locally Advanced Colorectal Cancer after Surgical Resection: A Multi-Center &amp; Multi-Omics study
Authors: Park Woong-Yang , Bang Yeong Hak , Choi Ji Hye , Park Kyunghee , Lim Jinyeong , Lee Boram , Han Kyung Yeon , Pyo Dae Hee , Cho Yong Beom , Kim Tae-You , Park Kyu Joo , Ryoo Seung-Bum , Kang Sung-Bum , Yu Chang Sik , Lee Jaeim , Lee Kil-yong , Kim Kyu-Tae , Lee Jin-Young , Kim Young-Joon 
Published: November 2023

Abstract
We aimed to predict postoperative prognosis of stage II/III colorectal cancer (CRC). Tumor-like normal microenvironment (tNME) and healthy normal microenvironment (hNME) groups were created using RNA sequencing (RNA-seq) data derived from 273 tumors and 273 paired normal tissues from margins of resected specimens. With a median follow-up of 58.2 months, the tNME group showed poor 5-year recurrence-free survival (54.7 vs. 73.0%, HR = 1.94, P = 0.002) and 5-year overall survival (78.2 vs. 83.0%, HR = 1.76, P = 0.033). Only the tNME group showed similar microbiome diversity between tumor and normal tissues. scRNA-seq revealed a positive co-occurrence of Th17, GZMKhighCD8+T cells, and IL1Bhigh neutrophils in the tumor and normal tissues of tNME. A high proportion of EMP1high epithelial cells was observed in the tNME group, with upregulated epithelial-mesenchymal transition and leukocyte signaling pathways. In conclusion, the microenvironmental status of normal tissues offers a promising biomarker for stage II/III CRC.
Effects of In-Service Strain on Girth Weld Failure of an X80 Pipeline
Authors: Chen Hongyuan , Xiongxiong Gao , Dongliang Yu , Hui Feng , Qiang Chi , Lei Wang , Chunyong Huo 
Published: November 2023

Abstract
In general, transmission pipelines are designed by hoop stress induced by internal pressure, and maximum longitudinal stress. The paper is mainly about the failure analysis of an X80 pipeline brittle fracture on girth weld under in-service strain. The digital image correlation (DIC) in the tensile tests was used to analyze the permanent plastic strain on girth weld metal under in-service strain. Then, the Charpy energy of the girth weld metal with the concentrated plastic strain was tested to evaluate the deterioration of toughness. Finally, the mechanism of the brittle fracture of the girth weld is clarified. The results show that the girth welds may have obvious plastic deformation under an elastic in-service strain of the pipeline. It may lead to a significant deterioration of the toughness, which results in the failure of the pipeline under the strain-controlled load.
Comparison between different lines reveals that Ipomoea cairica (L.) in mangrove wetlands acquires the ability to resist salt through phenotypic plasticity
Authors: Zou Jiatong , Yuan Benqi , Li Weihua , Xie xiaoting , Chen Minghao , Xiong Tiantian 
Published: November 2023

Abstract
Palmate-leaved morning glory (Ipomoea cairica (L.) Sweet) is a fast-growing perennial herbaceous twining vine that was recently discovered to invade mangrove wetlands in China. To understand the mechanism of its successful invasion, we compared the salt tolerance of a halophytic line from Zhuhai and a non-halophytic line from Guangzhou under salt stress. We measured morphological, physiological, and biochemical parameters related to growth, ion homeostasis, photosynthetic pigments, chlorophyll fluorescence parameters, oxidative stress, and apoptosis in both lines. Monitoring apoptosis showed that the halophytic line had a delayed protoplast apoptosis compared with the non-halophytic line. We also found that the halophytic line had higher stems that regenerated; lower water loss, Na+ uptake, and membrane damage; a higher density and area of salt glands; and better photosynthetic performance than the non-halophytic line. The halophyte prevented salt-related damage by reducing water loss and secreting excess sodium ions (Na+) through its lower stomatal density and higher density and area of salt glands. The halophytic line also maintained a better balance of Na+, potassium ions, nitrogen, and phosphorus under salt stress. The halophytic line had higher activities of antioxidant enzymes, including superoxide dismutase, peroxidase, and catalase, and non-enzymatic antioxidants of proline and anthocyanins, which indicate a stronger oxidative stress response. Our results suggest that the halophytic line adapts to higher salt tolerance than the non-halophytic line by enhancing its salt exclusion, osmolyte adjustment, and photosynthetic efficiency, which could explain its successful invasion in the mangrove wetland ecosystem.
Energy absorption characteristics of super-square chiral honeycomb
Authors: Wang Chengming , Deng Xiaolin 
Published: November 2023

Abstract
Most natural or biological materials exhibit by chiral and layered structures, which offer exceptional mechanical properties such as shear resistance, attenuation and impact resistance. Taking inspiration from circular chiral structures, the paper introduces a novel square chiral structure. A finite element numerical model was constructed using Abaqus/Explicit, and its accuracy was verified and a series of studies were carried out. Initially, a comparative analysis was performed to examine the mechanical properties and deformation modes of two structures: the super-square chiral honeycomb (SSCH) and the tetrachiral chiral honeycomb (TCH). For the same mass, the super-square chiral honeycomb has better energy absorption performance than the tetrachiral honeycomb, with SSCH absorbing 1.09 times the energy of TCH. Additionally, at the same mass, SSCH has a better deformation mode and lower initial peak force than TCH. Subsequently, a systematic parametric study of SSCH was carried out by varying the ratio of quadrilateral side lengths of square chiral structure ribs, wall thickness, and impact velocity. The structure investigated in this paper can provide a reference for the innovative design of the chiral honeycomb structure for specific applications.