bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the use of lake sedimentary DNA in palaeolimnology: A comparison with long‐term microscopy‐based monitoring of the phytoplankton community
Authors: Thorpe Amy C. , Mackay Eleanor B. , Goodall Tim , Bendle James A. , Thackeray Stephen J. , Maberly Stephen C. , Read Daniel S. 
Published: November 2023

AbstractPalaeolimnological records provide valuable information about how phytoplankton respond to long‐term drivers of environmental change. Traditional palaeolimnological tools such as microfossils and pigments are restricted to taxa that leave sub‐fossil remains, and a method that can be applied to the wider community is required. Sedimentary DNA (sedDNA), extracted from lake sediment cores, shows promise in palaeolimnology, but validation against data from long‐term monitoring of lake water is necessary to enable its development as a reliable record of past phytoplankton communities. To address this need, 18S rRNA gene amplicon sequencing was carried out on lake sediments from a core collected from Esthwaite Water (English Lake District) spanning ~105 years. This sedDNA record was compared with concurrent long‐term microscopy‐based monitoring of phytoplankton in the surface water. Broadly comparable trends were observed between the datasets, with respect to the diversity and relative abundance and occurrence of chlorophytes, dinoflagellates, ochrophytes and bacillariophytes. Up to 20% of genera were successfully captured using both methods, and sedDNA revealed a previously undetected community of phytoplankton. These results suggest that sedDNA can be used as an effective record of past phytoplankton communities, at least over timescales of <100 years. However, a substantial proportion of genera identified by microscopy were not detected using sedDNA, highlighting the current limitations of the technique that require further development such as reference database coverage. The taphonomic processes which may affect its reliability, such as the extent and rate of deposition and DNA degradation, also require further research.
Pan‐genome analysis highlights the role of structural variation in the evolution and environmental adaptation of Asian honeybees
Authors: Li Yancan , Yao Jun , Sang Huiling , Wang Quangui , Su Long , Zhao Xiaomeng , Xia Zhenyu , Wang Feiran , Wang Kai , Lou Delong , Wang Guizhi , Waterhouse Robert M. , Wang Huihua , Luo Shudong , Sun Cheng 
Published: November 2023

AbstractThe Asian honeybee, Apis cerana, is an ecologically and economically important pollinator. Mapping its genetic variation is key to understanding population‐level health, histories and potential capacities to respond to environmental changes. However, most efforts to date were focused on single nucleotide polymorphisms (SNPs) based on a single reference genome, thereby ignoring larger scale genomic variation. We employed long‐read sequencing technologies to generate a chromosome‐scale reference genome for the ancestral group of A. cerana. Integrating this with 525 resequencing data sets, we constructed the first pan‐genome of A. cerana, encompassing almost the entire gene content. We found that 31.32% of genes in the pan‐genome were variably present across populations, providing a broad gene pool for environmental adaptation. We identified and characterized structural variations (SVs) and found that they were not closely linked with SNP distributions; however, the formation of SVs was closely associated with transposable elements. Furthermore, phylogenetic analysis using SVs revealed a novel A. cerana ecological group not recoverable from the SNP data. Performing environmental association analysis identified a total of 44 SVs likely to be associated with environmental adaptation. Verification and analysis of one of these, a 330 bp deletion in the Atpalpha gene, indicated that this SV may promote the cold adaptation of A. cerana by altering gene expression. Taken together, our study demonstrates the feasibility and utility of applying pan‐genome approaches to map and explore genetic feature variations of honeybee populations, and in particular to examine the role of SVs in the evolution and environmental adaptation of A. cerana.
Forsythiaside A exhibits anti‐migration and anti‐inflammation effects in rheumatoid arthritis in vitro model
Authors: Su Minhui , Zhou Dinghua , Huang Jiamin , Yang Ting , Zhou Qi , Tan Yaju 
Published: November 2023

AbstractBackgroundRheumatoid arthritis (RA) is a kind of systemic autoimmune disease, and the joint inflammation and cartilage destruction are the major features. Some traditional Chinese medicine have been discovered to exhibit regulatory roles in the treatment of RA. Forsythiaside A (FA) as an active ingredient isolated from forsythia suspensa has been discovered to participate into the regulation of some diseases through improving inflammation. However, the regulatory effects of FA on the progression of RA keep indistinct.MethodsIL‐1β treatment (10 ng/mL) in MH7A cells was built to mimic RA in vitro (cell) model. The cell viability was examined through CCK‐8 assay. The cell proliferation was detected through Edu assay. The levels of TNF‐α, IL‐6, and IL‐8 were evaluated through ELISA. The protein expressions were measured through western blot. The cell apoptosis was assessed through flow cytometry. The cell migration and invasion abilities were tested through Transwell assay.ResultsIn this study, it was revealed that the cell proliferation was strengthened after IL‐1β treatment (p < .001), but this effect was reversed after FA treatment in a dose‐increasing manner (p < .05). Furthermore, FA suppressed inflammation in IL‐1β‐triggered MH7A cells through attenuating the levels of TNF‐α, IL‐6, and IL‐8 (p < .05). The cell apoptosis was lessened after IL‐1β treatment (p < .001), but this effect was rescued after FA treatment (p < .05). Besides, the cell migration and invasion abilities were both increased after IL‐1β treatment (p < .001), but these changes were offset after FA treatment (p < .05). Eventually, FA retarded the JAK/STAT pathway through reducing p‐JAK/JAK and p‐STAT/STAT levels (p < .01).ConclusionOur study manifested that FA exhibited anti‐migration and anti‐inflammation effects in RA in vitro model (IL‐1β‐triggered MH7A cells) through regulating the JAK/STAT pathway. This work hinted that FA can be an effective drug for RA treatment.
The challenges facing Chinese accounting scholars publishing in English (and Chinese) language journals
Authors: Dellaportas Steven , Xu Lina , Ji Sophia , Luo Dudu , Song Xiaoming 
Published: November 2023

AbstractInterviews with accounting scholars in China found that the pressure to publish in English language journals is high for reasons of tenure and promotion, but the expectation of acceptance is low. Publishing in Chinese language journals is equally as difficult because of socio‐cultural factors that favour a limited group of scholars with reputational capital. Scholars in China either avoid submitting articles to English language journals, collaborate with western scholars to enhance the probability of success, or focus on quantitative methodologies because of a perception that it is less demanding on language capability compared with qualitative approaches to research.
Socio‐economic inequalities in smoking and drinking in adolescence: Assessment of social network dynamics
Authors: Radó Márta K. , Kisfalusi Dorottya , Laverty Anthony A. , van Lenthe Frank J. , Been Jasper V. , Takács Károly 
Published: November 2023

AbstractAimsWe investigated whether (1) adolescents selected friends with a similar socio‐economic status (SES), (2) smoking and alcohol consumption spread in networks and (3) the exclusion of non‐smokers or non‐drinkers differed between SES groups.DesignThis was a longitudinal study using stochastic actor‐oriented models to analyze complete social network data over three waves.SettingEight Hungarian secondary schools with socio‐economically diverse classes took part.ParticipantsThis study comprised 232 adolescents aged between 14 and 15 years in the first wave.MeasurementsSelf‐reported smoking behavior, alcohol consumption behavior and friendship ties were measured. SES was measured based upon entitlement to an income‐tested regular child protection benefit.FindingsNon‐low‐SES adolescents were most likely to form friendships with peers from their own SES group [odds ratio (OR) = 1.07, 95% confidence interval (CI) = 1.02–1.11]. Adolescents adjusted their smoking behavior (OR = 24.05, 95% CI = 1.27–454.86) but not their alcohol consumption (OR = 1.65, 95% CI = 0.62–4.39) to follow the behavior of their friends. Smokers did not differ from non‐smokers in the likelihood of receiving a friendship nomination (OR = 0.98, 95% CI = 0.87–1.10), regardless of their SES. Alcohol consumers received significantly more friendship nominations than non‐consumers (OR = 1.16, 95% CI = 1.01–1.33), but this association was not significantly different according to SES.ConclusionsHungarian adolescents appear to prefer friendships within their own socio‐economic status group, and smoking and alcohol consumption spread within those friendship networks. Socio‐economic groups do not differ in the extent to which they encourage smoking or alcohol consumption.
Damage reduction of Acromyrmex lobicornis in Pinus ponderosa using mixed pine species in combination with push–pull tools
Authors: Alma Andrea M. , Fernández Patricia , Serra M. Noel , Villacide José , Buteler Micaela 
Published: November 2023

Abstract
Leaf‐cutting ants (LCAs) cause significant damage to forest plantations and are mainly managed by broad‐spectrum synthetic insecticides. In Patagonia, Pinus ponderosa (Pinales: Pinaceae) (Douglas 1836) and Pinus contorta (Pinales: Pinaceae) (Dluglas 1838) are the predominant pine species in forest plantations. Previous research has shown that dehydrated citrus pulp is an LCAs attractant, and tea tree essential oil acts as an LCAs repellent.
We evaluated the use of dehydrated citrus pulp and tea tree oil to manage LCAs behaviour in a push–pull setting, using mixed pine species in small plot fields, to reduce the LCA Acromyrmex lobicornis (Hymenoptera: Formicidae) (Emery, 1888) damage in young P. ponderosa plants. We combined the treatment with attractant and repellent in a mono‐specific plantation design (three ponderosa plants surrounded with pulp and three sprayed with tea tree extract) or mixed plantation designs (three contorta plants with pulp and three ponderosa plants with tea tree).
The combination of both mono‐specific and mixed plantations with push–pull stimuli delayed discovery and decreased the damage in pines sprayed with the repellent. Mono‐specific as well as mixed species designs were equally effective, reducing ant damage and increasing ~50% the survival probability of P. ponderosa when push–pull stimuli were applied.
The use of a push–pull strategy with plant‐based compounds provides potential as a management tool that reduces and delays LCA damage in pine trees.
Genome‐wide analyses based on a novel donkey 40K liquid chip reveal the gene responsible for coat color diversity in Chinese Dezhou donkey
Authors: Liu Shuqin , Su Jiangtian , Yang Qiwen , Sun Minhao , Wang Zhaofei , Yu Jie , Jafari Halima , Lei Chuzhao , Sun Yujiang , Dang Ruihua 
Published: November 2023

AbstractDezhou donkey is one of the representative local breeds in China, which is mainly divided into two strains: Sanfen and Wutou. There are obvious differences in coat color between the two strains. The former shows light points around the eyes, around the muzzle and under the belly, while the latter is completely solid black. In this study, genome‐wide association analysis was performed for the differences in coat color traits between the Sanfen (n = 97) and Wutou (n = 108) strains using a novel donkey 40K liquid chip developed based on GenoBaits technology, to identify genomic regions and causal genes that could explain this variation. We also used FST and The cross‐population composite likelihood ratio test (XPCLR) analyses to explore selected regions related to coat color differences. We identified one significant region on chromosome 15, with the most significant SNP located within the agouti signaling protein (ASIP) gene. At the same time, both FST and XPCLR methods detected the same selected region on chromosome 15, and ASIP was the gene with the strongest signal. ASIP and melanocortin 1 receptor (MC1R) control the ratio of eumelanin to pheomelanin through their protein activity. They are deeply involved in the process of melanosome organation and melanogenesis, thus affecting mammals’ coat color variation. We used a range of genome‐wide approach to identify the genetic basis of coat color variation in Dezhou donkeys. The results provide a supplement to the color variation study in Chinese donkeys at the genome‐wide level, and preliminarily verified the reliability of the Molbreeding Donkey No. 1 40K liquid chip.
Basic psychological needs: A framework for understanding childbirth satisfaction
Authors: Brand Rebecca J. , Gartland Casey A. 
Published: November 2023

AbstractWomen often report being dissatisfied with their childbirth experience, which in turn predicts negative outcomes for themselves and their children. Currently, there is no consensus as to what constitutes a satisfying or positive birth experience. We posit that a useful framework for addressing this question already exists in the form of Basic Psychological Needs Theory, a subtheory of Self‐Determination Theory (Deci & Ryan, Can. Psychol., 49, 2008, 182). Specifically, we argue that the degree to which maternity care practitioners support or frustrate women's needs for relatedness, competence, and autonomy predicts their childbirth satisfaction. Using this framework provides a potentially powerful lens to better understand and improve the well‐being of new mothers and their infants.
R‐CHOP treatment for patients with advanced follicular lymphoma: Over 15‐year follow‐up of JCOG0203
Authors: Watanabe Takashi , Tobinai Kensei , Wakabayashi Masaki , Maruyama Dai , Yamamoto Kazuhito , Kubota Nobuko , Shimada Kazuyuki , Asagoe Kohsuke , Yamaguchi Motoko , Ando Kiyoshi , Ogura Michinori , Kuroda Junya , Suehiro Youko , Matsuno Yoshihiro , Tsukasaki Kunihiro , Nagai Hirokazu 
Published: November 2023

SummaryAnti‐CD20 antibody in combination with chemotherapy extends overall survival (OS) in untreated advanced‐stage follicular lymphoma (FL), yet the optimal associated therapy is unclear. Data on the cumulative incidence of secondary malignancies postrelapse after conventional immunochemotherapy are scarce. A long‐term analysis of rituximab combined with cyclophosphamide, doxorubicin, vincristine and prednisone (R‐CHOP) as first‐line treatment was conducted in a randomised clinical trial. A six‐cycle R‐CHOP regimen was administered every 2 or 3 weeks without rituximab maintenance. A prespecified evaluation was conducted 15 years after the completion of enrolment, following initial analysis results that showed no significant differences in outcomes at the 3‐year mark. In‐depth analyses were performed on the cohort of 248 patients with FL who were allocated to the two treatment arms. With a median follow‐up period of 15.9 years, the 15‐year OS was 76.2%. There were no protocol treatment‐related deaths, nor were there any fatal infections attributable to subsequent lymphoma treatment. At 15 years, the cumulative incidence of non‐haematological and haematological malignancies was 12.8% and 3.7% respectively. Histological transformation appeared after a median of 8 years. R‐CHOP maintains safety and efficacy in patients with advanced FL over extended follow‐up, making it a viable first‐line option for patients with advanced‐stage FL.
Humanization: The humanistic perspective as a guide for supporting people with intellectual disability
Authors: Neuman Ran 
Published: November 2023

AbstractBackgroundDespite humanistic declarations regarding the rights of people with intellectual disability to live a full, meaningful life, in practice, resulting from dilemmas experienced by direct support provides, support is often limited to a focus on functional independence. The aim of this research was to define the theoretical principles by which the gap between humanistic declarations and practice can be overcome.MethodThe research focused on the role perception of 30 direct support providers who participated in semistructured interviews. A case study methodology was used applying an interpretative phenomenological analysis (IPA).FindingsThe interviews revealed that a humanistic perspective has been partially incorporated into the general attitudes of direct support providers. In practice, however, they indicated that they face dilemmas that challenge them in their daily work.ConclusionTo assist direct support providers in resolving their dilemmas, by adhering to humanistic principles, a ‘humanization’ model is offered. The theoretical principles underlying the model focus on meaningful life as the aim of support, enhancing recipients’ autonomy, adhering to a holistic perspective and conducting dialogues acknowledging the abilities of people with intellectual disability to understand, choose and face challenges. Further examination of the model and its application in practice is recommended.
Neon ion (20Ne10+) charged particle beams manipulate rapid tumor reoxygenation in syngeneic mouse models
Authors: Imaizumi Akiko , Hirayama Ryoichi , Ikoma Yoko , Nitta Nobuhiro , Obata Takayuki , Hasegawa Sumitaka 
Published: November 2023

AbstractCharged particle beams induce various biological effects by creating high‐density ionization through the deposition of energy along the beam's trajectory. Charged particle beams composed of neon ions (20Ne10+) hold great potential for biomedical applications, but their physiological effects on living organs remain uncertain. In this study, we demonstrate that neon‐ion beams expedite the process of reoxygenation in tumor models. We simulated mouse SCCVII syngeneic tumors and exposed them to either X‐ray or neon‐ion beams. Through an in vivo radiobiological assay, we observed a reduction in the hypoxic fraction in tumors irradiated with 8.2 Gy of neon‐ion beams 30 h after irradiation compared to 6 h post‐irradiation. Conversely, no significant changes in hypoxia were observed in tumors irradiated with 8.2 Gy of X‐rays. To directly quantify hypoxia in the irradiated living tumors, we utilized dynamic contrast‐enhanced magnetic resonance imaging (MRI) and diffusion‐weighted imaging. These combined MRI techniques revealed that the non‐hypoxic fraction in neon‐irradiated tumors was significantly higher than that in X‐irradiated tumors (69.53% vs. 47.67%). Simultaneously, the hypoxic fraction in neon‐ion‐irradiated tumors (2.77%) was lower than that in X‐irradiated tumors (4.27%) and non‐irradiated tumors (32.44%). These results support the notion that accelerated reoxygenation occurs more effectively with neon‐ion beam irradiation compared to X‐rays. These findings shed light on the physiological effects of neon‐ion beams on tumors and their microenvironment, emphasizing the therapeutic advantage of using neon‐ion charged particle beams to manipulate tumor reoxygenation.
Design and synthesis of imidazo[1,2‐a]pyridine‐chalcone conjugates as antikinetoplastid agents
Authors: Agarwal Devesh S. , Beteck Richard M. , Ilbeigi Kayhan , Caljon Guy , Legoabe Lesetja J. 
Published: November 2023

AbstractA library of imidazo[1,2‐a]pyridine‐appended chalcones were synthesized and characterized using 1H NMR, 13C NMR and HRMS. The synthesized analogues were screened for their antikinetoplastid activity against Trypanosoma cruzi, Trypanosoma brucei brucei, Trypanosoma brucei rhodesiense and Leishmania infantum. The analogues were also tested for their cytotoxicity activity against human lung fibroblasts and primary mouse macrophages. Among all screened derivatives, 7f was found to be the most active against T. cruzi and T. b. brucei exhibiting IC50 values of 8.5 and 1.35 μM, respectively. Against T. b. rhodesiense, 7e was found to be the most active with an IC50 value of 1.13 μM. All synthesized active analogues were found to be non‐cytotoxic against MRC‐5 and PMM with selectivity indices of up to more than 50.
Gender brilliance stereotype emerges early and predicts children's motivation in South Korea
Authors: Kim Seowoo , Jin Kyong‐sun , Bian Lin 
Published: November 2023

AbstractRecent work suggests that the stereotype associating brilliance with men may underpin women's underrepresentation in prestigious careers, yet little is known about its development and consequences in non‐Western contexts. The present research examined the onset of this stereotype and its relation to children's motivation in 5‐ to 7‐year‐old Korean children (N = 272, 50% girls, tested 2021 to 2022). At age 7, children attributed brilliance to men when evaluating Asians and Whites, and girls became less interested in participating in intellectually challenging tasks than boys. Notably, this gender difference in interest was mediated by children's endorsement of the stereotype. The generalizable early emergence of the gender brilliance stereotype and its detrimental implications press the need to tackle gender imbalance in early childhood.
Nonclassical congenital adrenal hyperplasia: Metabolic and hormonal profile
Authors: Piróg Magdalena , Pulka Anna , Zabiegło Ewa , Jach Robert 
Published: November 2023

AbstractObjectiveTo investigate both metabolic and hormonal profiles of untreated women with nonclassical congenital adrenal hyperplasia (NCCAH). The secondary objective was to compare above profiles with polycystic ovary syndrome (PCOS) women and healthy controls.DesignRetrospective, case‐control study.PatientsWomen assigned to one of the groups: (1) NCCAH (n = 216), (2) PCOS (n = 221), (3) regularly menstruating (n = 216).MeasurementsLipid profile including total cholesterol (TC), triglycerides (TG), low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol along with both fasting glucose (Glu) and insulin (Ins) levels and hormonal parameters were determined among all participants.ResultsBoth NCCAH and PCOS women had higher body mass index in comparison to the controls (+7% and 18.9%, respectively). NCCAH women exhibited higher TC (+34.1%) and fasting glucose levels (+18.9%) together with elevated testosterone (60.2%), dehydroepiandrosterone sulphate (28.1%), free androgen index (91.9%) and antimüllerian hormone (58%) in comparison to healthy controls. PCOS group showed unfavourably altered metabolic profile reflected by higher TC (+35.4%), TG (+25%), fasting Glu (+22%), fasting Ins (+34.4%) along with homoeostatic model assessment for insulin resistance (HOMA‐IR; 36.2%) in comparison to the controls. NCCAH women showed both lower insulin (−28.5%) and HOMA‐IR (−31.8%) levels when compared to the PCOS.ConclusionsNCCAH women showed less adversely altered metabolic profile than PCOS women, but not as favourable as in the healthy controls. Optimisation of screening for metabolic and reproductive health may help to initiate the treatment and improve treatment outcomes.