bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENERAPAN MODEL MIND MAPPING UNTUK MENINGKATKAN KREATIVITAS SISWA PADA PEMBELAJARAN TEMATIK KELAS IV SD NEGERI 33 KOTA TERNATE
Authors: Rustam Nabila , Hadi Darmawati , Ahsan Sukria 
Published: January 2024

Pembelajaran di kelas tidak terlepas dari peran guru sebagai pendidik, terdapat permasalahan didalam kelas diantaranya siswa yang belum bisa mengembangkan daya imajinasinya dalam artian kurang kreatif dan kurang peka terhadap keindahan dalam membuat catatan. Penelitian ini dilakukan dengan tujuan mengetahui peningkatan kreativitas siswa kelas IV SDN 33 Kota Ternate Pada pembelajaran tematik. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan kualitatif, jenis Penelitian Tindakan Kelas. Subjek penelitian siswa kelas IV SDN 33 Kota Ternate, yang berjumlah 23 siswa. Hasil penelitian, melalui penerapan model Mind Mapping terdapat peningkatan kreativitas siswa pada pembelajaran tematik. Peningkatan kreativitas siswa pada siklus I terdapat 11 siswa yang tuntas dan mencapai kategori kreatif, dengan persentase 47,8% standar KKM 70. Kemudian pada siklus II kreativitas siswa mengalami peningkatan, yakni terdapat 19 siswa yang mencapai kategori kreatif dengan persentase82,60%. Demikian, dapat disimpulkan bahwa penerapan model Mind Mapping dapat meningkatkankreativitas siswa kelas IV SDN 33 Kota Ternate.
SARS CoV 2 (COVID-19) Diagnosis in Wildlife Animals using Rt-Polymerase Chain Reaction (PCR) in Dubai Safari Park
Authors: H. Ghazaly Jameela H. x , Mandiram Jayadevan M. , Mustafa Murad B. , Alhajeri Muna A. 
Published: January 2024

Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses. SARS-CoV-2 is a beta coronavirus, a genus that includes several coronaviruses (SARS-CoV, MERS-CoV, bat SARS-like CoV, and others) isolated from humans, bats, camels, civets, and other animals. Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) is the pathogenic agent that causes the disease COVID-19. SARS-CoV-2 is thought to have emerged from an animal source, most likely a bat, and subsequently spread to humans. While genetically closely related viruses have been isolated from Rhinolophus bats, the exact source of SARS-CoV-2 and its route of introduction into the human population have not been established. Monitoring animal infections is imperative to better understanding their epidemiological significance for animal health, biodiversity, and human health. According to evidence from risk assessments, epidemiological investigations, and experimental studies, animals play no significant role in the spread of SARS-CoV-2, which is sustained by human-to-human transmission. The possibility of Coronavirus testing by veterinary labs was considered after the Iowa State veterinary lab discovered the COVID-19 virus has a similar DNA testing process to porcine epidemic diarrhea virus (PED). PED is another form of coronavirus that killed many piglets in 2013 and was unresponsive to treatment. Several vet labs optimized their equipment and processes to test for PED, helping them determine that older pigs could recover and develop immunity against the virus. Those same labs are still set up for coronavirus testing. We in Dubai Safari Park, particularly within the laboratory Veterinary Hospital, are conducting RT-PCR analysis to test diverse animal species including non-human primates, Carnivores, small mammals, and Ungulates. Even though none of our animals show any respiratory signs, we have conducted this study to ensure that our animal collection is healthy and free of SARS-COV-2.
Cinematic rendering improves the AO/OTA classification of distal femur fractures compared to volume rendering: a retrospective single-center study
Authors: Chen Song , Wang Xiong , Zheng Zhenxin , Fu Zhiqiang 
Published: January 2024

Purpose: Correctly classifying distal femur fractures is essential for surgical treatment planning and patient prognosis. This study assesses the potential of Cinematic Rendering (CR) in classifying these fractures, emphasizing its reported ability to produce more realistic images than Volume Rendering (VR).Methods: Data from 88 consecutive patients with distal femoral fractures collected between July 2013 and July 2020 were included. Two orthopedic surgeons independently evaluated the fractures using CR and VR. The inter-rater and intra-rater agreement was evaluated by using the Cicchetti-Allison weighted Kappa method. Accuracy, precision, recall, and F1 score were also calculated. Diagnostic confidence scores (DCSs) for both imaging methods were compared using chi-square or Fisher’s exact tests.Results: CR reconstruction yielded excellent inter-observer (Kappa = 0.989) and intra-observer (Kappa = 0.992) agreement, outperforming VR (Kappa = 0.941 and 0.905, respectively). While metrics like accuracy, precision, recall, and F1 scores were higher for CR, the difference was not statistically significant (p &gt; 0.05). However, DCAs significantly favored CR (p &lt; 0.05).Conclusion: CR offers a superior visualization of distal femur fractures than VR. It enhances fracture classification accuracy and bolsters diagnostic confidence. The high inter- and intra-observer agreement underscores its reliability, suggesting its potential clinical importance.
Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium
Authors: El-Beltagi Hossam S. , Halema Asmaa A. , Almutairi Zainab M. , Almutairi Hayfa Habes , Elarabi Nagwa I. , Abdelhadi Abdelhadi A. , Henawy Ahmed R. , Abdelhaleem Heba A. R. 
Published: January 2024

Lead pollution of the environment poses a major global threat to the ecosystem. Bacterial bioremediation offers a promising alternative to traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from industrial wastewater generated by heavily lead-containing industries. Eight lead-resistant strains were successfully isolated, and subsequently identified through molecular analysis. Among these, Enterobacter kobei FACU6 emerged as a particularly promising candidate, demonstrating an efficient lead removal rate of 83.4% and a remarkable lead absorption capacity of 571.9 mg/g dry weight. Furthermore, E. kobei FACU6 displayed a remarkable a maximum tolerance concentration (MTC) for lead reaching 3,000 mg/L. To further investigate the morphological changes in E. kobei FACU6 in response to lead exposure, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed. These analyses revealed significant lead adsorption and intracellular accumulation in treated bacteria in contrast to the control bacterium. Whole-genome sequencing was performed to gain deeper insights into E. kobei’s lead resistance mechanisms. Structural annotation revealed a genome size of 4,856,454 bp, with a G + C content of 55.06%. The genome encodes 4,655 coding sequences (CDS), 75 tRNA genes, and 4 rRNA genes. Notably, genes associated with heavy metal resistance and their corresponding regulatory elements were identified within the genome. Furthermore, the expression levels of four specific heavy metal resistance genes were evaluated. Our findings revealed a statistically significant upregulation in gene expression under specific environmental conditions, including pH 7, temperature of 30°C, and high concentrations of heavy metals. The outstanding potential of E. kobei FACU6 as a source of diverse genes related to heavy metal resistance and plant growth promotion makes it a valuable candidate for developing safe and effective strategies for heavy metal disposal.
Anti-obesity effects by parasitic nematode (Trichinella spiralis) total lysates
Authors: Kang Shin Ae , Yu Hak Sun 
Published: January 2024

BackgroundObesity is an inducible factor for the cause of chronic diseases and is described by an increase in the size and number of adipocytes that differentiate from precursor cells (preadipocytes). Parasitic helminths are the strongest natural trigger of type 2 immune system, and several studies have showed that helminth infections are inversely correlated with metabolic syndromes.Methodology/Principal findingsTo investigate whether helminth-derived molecules have therapeutic effects on high-fat diet (HFD)-induced obesity, we isolated total lysates from Trichinella spiralis muscle larvae. We then checked the anti-obesity effect after intraperitoneal administration and intraoral administration of total lysate from T. spiralis muscle larvae in a diet-induced obesity model. T. spiralis total lysates protect against obesity by inhibiting the proinflammatory response and/or enhancing M2 macrophages. In addition, we determined the effects of total lysates from T. spiralis muscle larvae on anti-obesity activities in 3T3-L1 preadipocytes by investigating the expression levels of key adipogenic regulators, including peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT-enhancer-binding protein alpha (C/EBPα) and adipocyte protein 2 (aP2). Oil Red O staining showed that the total lysates from T. spiralis muscle larvae decreased the differentiation of 3T3-L1 preadipocytes by decreasing the number of lipid droplets. In addition, the production levels of proinflammatory cytokines IL-1β, IL-6, IFN-γ and TNF-α were examined by enzyme-linked immunosorbent assay (ELISA). T. spiralis total lysates decreased intracellular lipid accumulation and suppressed the expression levels of PPARγ, C/EBPα and aP2.Conclusion/SignificanceThese results show that T. spiralis total lysate significantly suppresses the symptoms of obesity in a diet- induced obesity model and 3T3-L1 cell differentiation and suggest that it has potential for novel anti-obesity therapeutics.
In vitro antibacterial activity of antiretroviral drugs on key commensal bacteria from the human microbiota
Authors: Rubio-Garcia Elisa , Ferrando Núria , Martin Núria , Ballesté-Delpierre Clara , Miró Jose M. , Paredes Roger , Casals-Pascual Climent , Vila Jordi 
Published: January 2024

IntroductionAntiretroviral therapy has improved life expectancy in HIV-infected patients. However, people living with HIV under antiretroviral therapy are at higher risks of developing chronic complications and acquiring multidrug resistant bacteria than healthy population. These factors have been associated with shifts in gut microbiome composition and immune activation. It is unclear how antiretroviral drugs affect gut microbiota composition, but it has been observed that antiretroviral treatment is not able to fully restore gut health after HIV infection. Additionally, some antiretroviral drugs have shown antibacterial activity suggesting that these drugs could have a direct impact on the human microbiome composition. MethodsWe determined the in vitro antibacterial activity of 16 antiretroviral drugs against a set of key clinically relevant and human commensal bacterial strains. ResultsOur results demonstrate that 5 antiretroviral drugs have in vitro antibacterial activity against gut and vaginal human commensal bacteria. Zidovudine has antibacterial activity against Escherichia coli, Klebsiella pneumoniae and Prevotella bivia, abacavir against Gardnerella vaginalis, efavirenz against G. vaginalis and P. bivia and bictegravir against Enterococcus spp. and G. vaginalis. Moreover, we describe for the first time that elvitegravir has antibacterial activity against G. vaginalis and P. bivia and, most importantly, against vancomycin-resistant Enterococcus spp. and methicillin-resistant Staphylococcus aureus strains with MIC values of 4-16 and 4 µg/mL, respectively showing high level of effectiveness against the tested multidrug-resistant bacteria.DiscussionOur results underscore that some antiretroviral drugs may influence the human microbiota composition. In addition, we report the potential use of elvitegravir to treat multidrug-resistant Gram-positive bacteria warranting the need of clinical studies to repurpose this antiretroviral drug.
Multiple cross displacement amplification combined with nanoparticle-based lateral flow biosensor for rapid and sensitive detection of Epstein-Barr virus
Authors: Jia Xinbei , Zhou Juan , Xiao Fei , Huang Xiaolan , He Wenqiang , Hu Wen , Kong Yaru , Yan Weiheng , Ji Jie , Qi Yuwei , Wang Yi , Tai Jun 
Published: January 2024

IntroductionEpstein-Barr virus (EBV) is a highly dangerous virus that is globally prevalent and closely linked to the development of nasopharyngeal cancer (NPC). Plasma EBV DNA analysis is an effective strategy for early detection, prognostication and monitoring of treatment response of NPC.MethodsHere, we present a novel molecular diagnostic technique termed EBV-MCDA-LFB, which integrates multiple cross displacement amplification (MCDA) with nanoparticle-based lateral flow (LFB) to enable simple, rapid and specific detection of EBV. In the EBV-MCDA-LFB system, a set of 10 primers was designed for rapidly amplifying the highly conserved tandem repeat BamHI-W region of the EBV genome. Subsequently, the LFB facilitate direct assay reading, eliminating the use of extra instruments and reagents.ResultsThe outcomes showed that the 65°C within 40 minutes was the optimal reaction setting for the EBV-MCDA system. The sensitivity of EBV-MCDA-LFB assay reached 7 copies per reaction when using EBV recombinant plasmid, and it showed 100% specificity without any cross-reactivity with other pathogens. The feasibility of the EBV-MCDA-LFB method for EBV detection was successfully validated by 49 clinical plasma samples. The complete detection process, consisting of rapid template extraction (15 minutes), MCDA reaction (65°C for 40 minutes), and LFB result reading (2 minutes), can be finalized within a 60-minutes duration.DiscussionEBV-MCDA-LFB assay designed here is a fast, extremely sensitive and specific technique for detecting EBV in field and at the point-of-care (PoC), which is especially beneficial for countries and regions with a high prevalence of the disease and limited economic resources.
Utility of electrocardiogram to predict the occurrence of the no-reflow phenomenon in patients undergoing primary percutaneous coronary intervention (PPCI): a systematic review and meta-analysis
Authors: Jafari Afshar Elmira , Gholami Niloofar , Samimisedeh Parham , MozafaryBazargany MohammadHossein , Tayebi Amirhossein , Memari Amirhossein , Yazdani Shahrooz , Rastad Hadith 
Published: January 2024

BackgroundThe no-reflow phenomenon affects about one out of five patients undergoing Primary Percutaneous Coronary Intervention (PPCI). As the prolonged no-reflow phenomenon is linked with unfavorable outcomes, making early recognition is crucial for effective management and improved clinical outcomes in these patients. Our review study aimed to determine whether electrocardiogram (ECG) findings before PCI could serve as predictors for the occurrence of the no-reflow phenomenon.Methods and materialsWe systematically searched MEDLINE, Scopus, and Embase to identify relevant studies. The random-effect model using inverse variance and Mantel-Haenszel methods were used to pool the standardized mean differences (SMD) and odds ratios (OR), respectively.ResultSixteen eligible articles (1,473 cases and 4,264 controls) were included in this study. Based on our meta-analysis of baseline ECG findings, the no-reflow group compared to the control group significantly had a higher frequency of fragmented QRS complexes (fQRS) (OR (95% CI): 1.35 (0.32–2.38), P-value = 0.01), and Q-waves (OR (95% CI): 1.97 (1.01–2.94), P-value &lt;0.001). Also, a longer QRS duration (QRSD) (SMD (95% CI): 0.72 (0.21, 1.23), p-value &lt;0.001) and R wave peak time (RWPT) (SMD (95% CI): 1.36 (0.8, 1.93), P &lt; 0.001) were seen in the no-reflow group. The two groups had no significant difference regarding P wave peak time (PWPT), and P wave maximum duration (Pmax) on baseline ECG.ConclusionOur findings suggest that prolonged QRSD, delayed RWPT, higher fQRS prevalence, and the presence of a Q wave on baseline ECG may predict the occurrence of the no-reflow phenomenon in patients undergoing PPCI.
A more-Comers populAtion trEated with an ultrathin struts polimer-free Sirolimus stent: an Italian post-maRketing study (the CAESAR registry)
Authors: Tarantini Giuseppe , Cardaioli Francesco , De Iaco Giuseppe , Tuccillo Bernardino , De Angelis Maria Carmen , Mauro Ciro , Boccalatte Marco , Trivisonno Antonio , Ribichini Flavio , Vadalà Giuseppe , Caramanno Giuseppe , Caruso Marco , Lombardi Mario , Fischetti Dionigi , Danesi Alessandro , Abbracciavento Leonardo , Lorenzoni Giulia , Gregori Dario , Panza Andrea , Nai Fovino Luca , Esposito Giovanni 
Published: January 2024

IntroductionThe use of contemporary drug-eluting stents (DES) has significantly improved outcomes of patients with coronary artery disease (CAD) undergoing percutaneous coronary intervention (PCI). However, concerns exist regarding the long-term proinflammatory effects of durable polymer coatings used in most DES, potentially leading to long-term adverse events. First-generation polymer-free stent technologies, such as sirolimus- and probucol-eluting stents (PF-SES), have shown an excellent safety and efficacy profile. The aim of this study was to evaluate the safety and efficacy of the new ultrathin Coroflex ISAR NEO PF-SES, in a more-comers PCI population.MethodsThe CAESAR (a more-Comers populAtion trEated with an ultrathin struts polimer-free Sirolimus stent: An Italian post-maRketing study) registry is a multicenter, prospective study conducted in Italy, enrolling more-comers CAD patients undergoing PCI with the Coroflex ISAR NEO stent. Patients with left main (LM) disease, cardiogenic shock (CS), or severely reduced left-ventricular ejection fraction (LVEF) were excluded. The primary endpoint was target-lesion revascularization (TLR) at 1 year.ResultsA total of 425 patients were enrolled at 13 centers (mean age 66.9 ± 11.6 years, Diabetes mellitus 29%, acute coronary syndrome 67%, chronic total occlusion 9%). Of these, 40.9% had multivessel disease (MVD) and in 3.3% cases, the target lesion was in-stent restenosis (ISR). Clinical device success was reached in 422 (99.6%) cases. At 1 year, only two (0.5%) subjects presented ischemia-driven TLR. The 1-year rates of target vessel revascularization and MACE were 0.5% and 5.1%, respectively. Major bleeding was observed in four (1.0%) patients.ConclusionIn this multicenter, prospective registry, the use of a new ultrathin Coroflex ISAR NEO PF-SES in a more-comers PCI population showed good safety and efficacy at 1 year.
Lipopolysaccharide-induced chronic inflammation increases female serum gonadotropins and shifts the pituitary transcriptomic landscape
Authors: Garcia Christopher , Velez Leandro M. , Ujagar Naveena , Del Mundo Zena , Nguyen Thu , Fox Chelsea , Mark Adam , Fisch Kathleen M. , Lawson Mark A. , Duleba Antoni J. , Seldin Marcus M. , Nicholas Dequina A. 
Published: January 2024

IntroductionFemale reproductive function depends on a choreographed sequence of hormonal secretion and action, where specific stresses such as inflammation exert profound disruptions. Specifically, acute LPS-induced inflammation inhibits gonadotropin production and secretion from the pituitary, thereby impacting the downstream production of sex hormones. These outcomes have only been observed in acute inflammatory stress and little is known about the mechanisms by which chronic inflammation affects reproduction. In this study we seek to understand the chronic effects of LPS on pituitary function and consequent luteinizing and follicle stimulating hormone secretion.MethodsA chronic inflammatory state was induced in female mice by twice weekly injections with LPS over 6 weeks. Serum gonadotropins were measured and bulk RNAseq was performed on the pituitaries from these mice, along with basic measurements of reproductive biology.ResultsSurprisingly, serum luteinizing and follicle stimulating hormone was not inhibited and instead we found it was increased with repeated LPS treatments.DiscussionAnalysis of bulk RNA-sequencing of murine pituitary revealed paracrine activation of TGFβ pathways as a potential mechanism regulating FSH secretion in response to chronic LPS. These results provide a framework with which to begin dissecting the impacts of chronic inflammation on reproductive physiology.
Correlation of mild cognitive impairment with the thickness of retinal nerve fiber layer and serum indicators in type 2 diabetic patients
Authors: Li Renshi , Zheng Fengjie , Xu Peichen , Lv Li , Mu Yapeng , Zhuang Xianghua , Chen Shihong 
Published: January 2024

BackgroundCognitive Impairment arising from type 2 diabetes mellitus (T2DM) has garnered significant attention in recent times. However, there are few studies on the identification and diagnosis of markers of cognitive impairment. Notably, alterations in the Retinal Nerve Fiber Layer’s (RNFL) thickness can potentially serve as an indicative measure of central nervous system changes. Further investigations have indicated that the decline in cognitive function within T2DM patients is intricately linked to persistent systemic inflammation and the accumulation of advanced glycosylation end products. Comprehensive studies are warranted to unveil these complex associations.ObjectiveThis study aims to explore the potential of utilizing the RNFL thickness and serological concentrations of IL-18, irisin, CML, and RAGE as diagnostic indicators for Mild Cognitive Impairment (MCI) among individuals with T2DM.MethodsThe thickness of RNFL were determined in all patients and controls using optical coherence tomography (OCT). The serum levels of IL-18, irisin, CML and RAGE were detected by ELISA kit. In addition, Cognitive assessment was performed by the Mini-Mental State Examination (MMSE) and the Montreal Cognitive assessment (MoCA).ResultsThe average RNFL thickness in the right eye were decreased in T2DM and T2DM combined with MCI (T2DM-MCI) patients and were positively correlated with MoCA and MMSE scores. The serum levels of IL-18, CML and RAGE in T2DM and T2DM-MCI increased significantly (p&lt;0.05) and were negative correlated with MoCA and MMSE scores. The level of irisin in T2DM and T2DM-MCI decreased significantly (p&lt;0.05) and were positively correlated with MoCA and MMSE scores. The area under the ROC curve of T2DM-MCI predicted by the average RNFL thickness in the right eye, CML and RAGE were 0.853, 0.874 and 0.815. The diagnostic efficacy of the combination of average RNFL thickness in the right eye, CML, and RAGE for the diagnosis of T2DM-MCI was 0.969.ConclusionThe average RNFL thickness in the right eye, CML and RAGE have possible diagnostic value in T2DM-MCI patients.