bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validity of a Common Measure of Intimate Partner Violence Perpetration: Impact on Study Inference in Trials in Low- and Middle-Income Countries
Authors: Clark Cari Jo , Bergenfeld Irina , Shervinskie Abbie , Johnson Erin R. , Cheong Yuk Fai , Kaslow Nadine J , Yount Kathryn M 
Published: January 2024

ABSTRACTBackgroundIn lower-and middle-income countries (LMICs), studies of interventions to reduce intimate partner violence (IPV) perpetration are expanding, yet measurement equivalence of the IPV perpetration construct that is the primary outcome in these investigations has not been established. We assessed the measurement equivalence of physical and sexual IPV perpetration item sets used in recent trials in LMICs and tested the impact of non-invariance on trial inference.MethodsWith data from three intervention trials among men (sample size 505-1537 across studies) completed in 2019, we calculated tetrachoric correlations among items and used multiple-group confirmatory factor analysis to assess invariance across arms and over time. We also assessed treatment effects adjusting for covariate imbalance and using inverse probability to treatment weights to assess concordance of invariant measures with published results, where warranted.FindingsThe average correlation among items measuring IPV perpetration was high and increased by 0.03 to 0.15 for physical IPV and 0.07 to 0.17 for sexual IPV over time with several items in two studies showing correlations ≥ 0.85 at endline. Increases in the degree of correlation for physical IPV were concentrated in the treatment arm in two of the studies. The increase in correlation in sexual IPV differed by arm across studies. Across all studies, a correlated two-factor solution was the best fitting model according to the EFAs and CFAs. One study demonstrated measurement invariance across arms and over time. In two of the studies, longitudinal measurement non-invariance was detected in the intervention arms. In post hoc testing, one study attained invariance with a one-factor model and study inference was concordant with published findings. The other study did not attain even partial invariance.ConclusionCommon measures of physical and sexual IPV perpetration cannot be used validly for comparisons across treatment versus control groups over time without further refinement. The study highlights the need for an expanded item set, content validity assessments, further measurement invariance testing, and then consistent use of the item sets in future intervention trials to ensure valid inferences regarding the effectiveness of IPV perpetration prevention interventions within and across trials.
Multi-conflict islands are a widespread trend withinSerratiaspp
Authors: Cummins Thomas , Garrett Stephen R. , Blower Tim R. , Mariano Giuseppina 
Published: January 2024

SUMMARYBacteria carry numerous anti-phage systems in ‘defence islands’ or hotspots. Recent studies have delineated the content and boundaries of these islands in various species, revealing instances of islands that encode additional factors, including antibiotic resistance, stress genes, Type VI Secretion System (T6SS)-dependent effectors, and virulence factors.Our study identifies three defence islands in theSerratiagenus with a mixed cargo of anti-phage systems, virulence factors and different types of anti-bacterial modules, revealing a widespread trend of co-accumulation that extends beyond T6SS-dependent effectors to colicins and contact-dependent inhibition systems. We report the identification of four distinct anti-phage system/subtypes, including a previously unreported Toll/IL-1 receptor (TIR)-domain-containing system with population-wide immunity, and two loci co-opting a predicted T6SS-related protein for phage defence. This study enhances our understanding of the protein domains that can be co-opted for phage defence and of the diverse combinations in which known anti-phage proteins can be assembled, resulting in a highly diversified anti-phage arsenal.
Assembly, stability, and dynamics of the infant gut microbiome are linked to bacterial strains and functions in mother’s milk
Authors: Allert Mattea , Ferretti Pamela , Johnson Kelsey E. , Heisel Timothy , Gonia Sara , Knights Dan , Fields David A. , Albert Frank W. , Demerath Ellen W. , Gale Cheryl A. , Blekhman Ran 
Published: January 2024

AbstractThe establishment of the gut microbiome in early life is critical for healthy infant development. Although human milk is recommended as the sole source of nutrition for the human infant, little is known about how variation in milk composition, and especially the milk microbiome, shapes the microbial communities in the infant gut. Here, we quantified the similarity between the maternal milk and the infant gut microbiome using 507 metagenomic samples collected from 195 mother-infant pairs at one, three, and six months postpartum. We found that the microbial taxonomic overlap between milk and the infant gut was driven by bifidobacteria, in particular byB. longum. Infant stool samples dominated byB. longumalso showed higher temporal stability compared to samples dominated by other species. We identified two instances of strain sharing between maternal milk and the infant gut, one involving a commensal (B. longum) and one a pathobiont (K. pneumoniae). In addition, strain sharing between unrelated infants was higher among infants born at the same hospital compared to infants born in different hospitals, suggesting a potential role of the hospital environment in shaping the infant gut microbiome composition. The infant gut microbiome at one month compared to six months of age was enriched in metabolic pathways associated withde-novomolecule biosynthesis, suggesting that early colonisers might be more versatile and metabolically independent compared to later colonizers. Lastly, we found a significant overlap in antimicrobial resistance genes carriage between the mother’s milk and their infant’s gut microbiome. Taken together, our results suggest that the human milk microbiome has an important role in the assembly, composition, and stability of the infant gut microbiome.
Hypoxia-induced Complement Component 3 Promotes Aggressive Tumor Growth in the Glioblastoma Microenvironment
Authors: Rosberg Rebecca , Smolag Karolina I. , Sjölund Jonas , Johansson Elinn , Bergelin Christina , Wahldén Julia , Pantazopoulou Vasiliki , Ceberg Crister , Pietras Kristian , Blom Anna M. , Pietras Alexander 
Published: January 2024

AbstractGlioblastoma (GBM) is the most aggressive form of glioma with a high rate of relapse despite intensive treatment. Tumor recurrence is tightly linked to radio-resistance, which in turn is associated with hypoxia. Here, we discovered a strong link between hypoxia and local complement signaling using publicly available bulk, single cell, and spatially resolved transcriptomic data from human GBM patients. Complement component 3 (C3) and the receptorC3AR1were both associated with aggressive disease and shorter survival in human glioma. In a genetically engineered mouse model of GBM, we found C3 specifically in hypoxic tumor areas. In vitro, we found an oxygen level-dependent increase inC3andC3AR1expression in response to hypoxia in several GBM and stromal cell types. Presence of C3 increased proliferation of GBM cells under hypoxic conditions, as well as clonal survival of GBM cells following radiation. Targeting C3aR using the antagonist SB290157 decreased GBM cell self-renewal in vitro, and prolonged survival of glioma bearing mice both alone and in combination with radiotherapy while reducing the number of M2-polarized macrophages. Our findings establish a strong link between hypoxia and complement pathways in GBM, and support a role of hypoxia-induced C3a-C3aR signaling as a contributor to glioma aggressiveness.
Temporal epigenome modulation enables efficient bacteriophage engineering and functional analysis of phage DNA modifications
Authors: Pozhydaieva Nadiia , Billau Franziska Anna , Wolfram-Schauerte Maik , Rojas Adán Andrés Ramírez , Paczia Nicole , Schindler Daniel , Höfer Katharina 
Published: January 2024

AbstractLytic bacteriophages hold substantial promise in medical and biotechnological applications. CRISPR-Cas systems offer a way to explore these mechanisms via site-specific phage mutagenesis. However, phages can resist Cas-mediated cleavage through extensive DNA modifications like cytosine glycosylation, hindering mutagenesis efficiency. Our study utilizes the eukaryotic enzyme NgTET to temporarily reduce phage DNA modifications, facilitating Cas nuclease cleavage and enhancing mutagenesis efficiency. This approach enables precise DNA targeting and seamless point mutation integration, exemplified by deactivating specific ADP-ribosyltransferases crucial for phage infection. Furthermore, by temporally removing DNA modifications, we elucidated the effects of these modifications on T4 phage infections without necessitating gene deletions.Our results present a strategy enabling the investigation of phage epigenome functions and streamlining the engineering of phages with cytosine DNA modifications. The described temporal modulation of the phage epigenome is valuable for synthetic biology and fundamental research to comprehend phage infection mechanisms through the generation of mutants.
Rapid and objective assessment of auditory temporal processing using dynamic amplitude-modulated stimuli
Authors: Parida Satyabrata , Yurasits Kimberly , Cancel Victoria E. , Zink Maggie E. , Mitchell Claire , Ziliak Meredith C. , Harrison Audrey V. , Bartlett Edward L. , Parthasarathy Aravindakshan 
Published: January 2024

AbstractAuditory neural coding of speech-relevant temporal cues can be noninvasively probed using envelope following responses (EFRs), neural ensemble responses phase-locked to the stimulus amplitude envelope. EFRs emphasize different neural generators, such as the auditory brainstem or auditory cortex, by altering the temporal modulation rate of the stimulus. EFRs can be an important diagnostic tool to assess auditory neural coding deficits that go beyond traditional audiometric estimations. Existing approaches to measure EFRs use discrete amplitude modulated (AM) tones of varying modulation frequencies, which is time consuming and inefficient, impeding clinical translation. Here we present a faster and more efficient framework to measure EFRs across a range of AM frequencies using stimuli that dynamically vary in modulation rates, combined with spectrally specific analyses that offer optimal spectrotemporal resolution. EFRs obtained from several species (humans, Mongolian gerbils, Fischer-344 rats, and Cba/CaJ mice) showed robust, high-SNR tracking of dynamic AM trajectories (up to 800Hz in humans, and 1.4 kHz in rodents), with a fivefold decrease in recording time and thirtyfold increase in spectrotemporal resolution. EFR amplitudes between dynamic AM stimuli and traditional discrete AM tokens within the same subjects were highly correlated (94% variance explained) across species. Hence, we establish a time-efficient and spectrally specific approach to measure EFRs. These results could yield novel clinical diagnostics for precision audiology approaches by enabling rapid, objective assessment of temporal processing along the entire auditory neuraxis.
Astrocytic tau inclusions lead to microglial abnormalities, but leave neuronal networks intact
Authors: Vogels Thomas , Vargová Gréta , Hromádka Tomáš 
Published: January 2024

AbstractAstrocytic tau inclusions are commonly found in the aging brain and primary tauopathies, such as progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD). The functional consequences of these histopathological lesions, however, are unknown due to the lack of specific animal models. We have developed a mouse model of astrocytic tau pathology to study downstream effects on microglia and neuronal networks. We have designed an adeno-associated viral (AAV) vector expressing aggregation-prone human truncated tau (amino acids 151-391/4R) and red fluorophore mCherry in equal ratio under the astrocytic GFAP promotor. Injection of AAV-GFAP-htTau into the hippocampus of wild-type mice led to expression of human truncated tau in astrocytes and accumulation of soluble phosphorylated tau (p214, p231) but no detectable cognitive impairment. In vivo multiphoton imaging revealed alterations in microglial morphology in the vicinity of truncated tau positive astrocytes in the cortex. No alterations in firing patterns of excitatory cortical neurons surrounded by astrocytes overexpressing truncated tau were detected. These results suggest that early stages of astrocytic tau pathology lead to changes in microglial function, but not to functional impairment of neuronal networks.
Formation of long-term memory without short-term memory by CaMKII inhibition
Authors: Shin Myung Eun , Parra-Bueno Paula , Yasuda Ryohei 
Published: January 2024

AbstractMost of the current models of long-term memory consolidation require prior establishment of short-term memory. Here, we show that optogenetic or genetic inhibition of CaMKII, a kinase important for synaptic plasticity, in an inhibitory avoidance task impairs short-term memory at 1 h but not long-term memory at 1 d. Similarly, cortico-amygdala synaptic potentiation was more sensitive to CaMKII inhibition at 1 h but not at 1 d. These results strongly suggest that long-term memory does not require the prior formation of short-term memory and that CaMKII-dependent synaptic plasticity specifically regulates short-term memory, but not long-term memory, for avoidance memory.
Evidence for interacting but decoupled controls of decisions and movements in non-human primates
Authors: Saleri Clara , Thura David 
Published: January 2024

AbstractMany recent studies indicate that control of decisions and actions is integrated during interactive behavior. Among these, several carried out in humans and monkeys conclude that there is a co-regulation of choices and movements. Another perspective, based on human data only, proposes a decoupled control of decision duration and movement speed, allowing for instance to trade decision duration for movement duration when time pressure increases. Crucially, it is not currently known whether this ability to flexibly dissociate decision duration from movement speed is specific to humans, whether it can vary depending on the context in which a task is performed, and whether it is stable over time. These are important questions to address, especially to rely on monkey electrophysiology to infer the neural mechanisms of decision-action coordination in humans. To do so, we trained two macaque monkeys in a perceptual decision-making task and analyzed data collected over multiple behavioral sessions. Our findings reveal a strong and complex relationship between decision duration and movement vigor. Decision duration and action duration can co-vary but also “compensate” each other. Such integrated but decoupled control of decisions and actions aligns with recent studies in humans, validating the monkey model in electrophysiology as a means of inferring neural mechanisms in humans. Crucially, we demonstrate for the first time that this control can evolve with experience, in an adapted manner. Together, the present findings contribute to deepening our understanding of the integrated control of decisions and actions during interactive behavior.New & noteworthyThe mechanism by which the integrated control of decisions and actions occurs, coupled or interactive but decoupled, is debated. In the present study, we show in monkeys that decisions and actions influence each other in a decoupled way. For the first time, we also demonstrate that this control can evolve depending the subject’s experience, allowing to trade movement time for decision time and limit the temporal discounting of reward value.
Expression patterns of FGF and BMP pathway genes in the tardigradeHypsibius exemplaris
Authors: Heikes Kira L. , Goldstein Bob 
Published: January 2024

AbstractA small number of conserved signaling pathways regulate development of most animals, yet we do not know where these pathways are deployed in most embryos. This includes tardigrades, a phylum with a unique body shape. We examined expression patterns of components of the BMP and FGF signaling pathways during embryonic segmentation and mesoderm development of the tardigradeHypsibius exemplaris. Among the patterns examined, we found that an FGF ligand gene is expressed in ectodermal segment posteriors and an FGF receptor gene is expressed in underlying endomesodermal pouches, suggesting possible FGF signaling between these developing germ layers. We found that a BMP ligand gene is expressed in lateral ectoderm and dorsolateral bands along segment posteriors, while the BMP antagonist Sog gene is expressed in lateral ectoderm and also in a subset of endomesodermal cells, suggesting a possible role of BMP signaling in dorsal-ventral patterning of lateral ectoderm. In combination with known roles of these pathways during development of common model systems, we developed hypotheses for how the BMP and FGF pathways might regulate embryo segmentation and mesoderm formation of the tardigradeH. exemplaris. These results identify the expression patterns of genes from two conserved signaling pathways for the first time in the tardigrade phylum.
Neuronal temporal filters as normal mode extractors
Authors: Golkar Siavash , Berman Jules , Lipshutz David , Haret Robert Mihai , Gollisch Tim , Chklovskii Dmitri B. 
Published: January 2024

Physical Review Research
To generate actions in the face of physiological delays, the brain must predict the future. Here we explore how prediction may lie at the core of brain function by considering a neuron predicting the future of a scalar time series input. Assuming that the dynamics of the lag vector (a vector composed of several consecutive elements of the time series) are locally linear, normal mode decomposition decomposes the dynamics into independently evolving (eigen)modes allowing for straightforward prediction. We propose that a neuron learns the top mode and projects its input onto the associated subspace. Under this interpretation, the temporal filter of a neuron corresponds to the left eigenvector of a generalized eigenvalue problem. We mathematically analyze the operation of such an algorithm on noisy observations of synthetic data generated by a linear system. Interestingly, the shape of the temporal filter varies with the signal-to-noise ratio (SNR): a noisy input yields a monophasic filter and a growing SNR leads to multiphasic filters with progressively greater number of phases. Such variation in the temporal filter with input SNR resembles that observed experimentally in biological neurons.Published by the American Physical Society2024
Hall response of locally correlated two-dimensional electrons at low density
Authors: Morpurgo Giacomo , Rademaker Louk , Berthod Christophe , Giamarchi Thierry 
Published: January 2024

We study the Hall constant in a homogeneous two-dimensional fluid of correlated electrons immersed in a perpendicular magnetic field, with special focus on the regime of low carrier density. The model consists of a one-band tight-binding model and a momentum-independent causal self-energy, representing interaction-induced correlations effects that are restricted to be local in space. We write general gauge-invariant equations for the conductivity tensor at first order in the magnetic field and solve them numerically—–analytically when possible—in a minimal model of anisotropic square lattice with constant self-energy. Our results show that deviations from the universal behavior of the Hall constant, as observed in the semiclassical regime, appear upon entering the quantum regime, where the Fermi energy and interaction are comparable energy scales.

Published by the American Physical Society
2024
Corporate social responsibility in the Brazilian ceramic tile industry
Authors: Scur Gabriela , Pião Roberta de Castro Souza , De Souza William Jeferson Vieira , De Melo Mary Fernanda de Sousa 
Published: January 2024

PurposeThe ceramic tile chain can generate several environmental impacts, from raw material extraction to the production process, commercialization, consumption, maintenance and disposal. One of the open issues remaining in the literature on socially responsible supply chains, also known as responsible supply chains, is understanding how corporate social responsibility (CSR) practices are being implemented in ceramic tile companies in Brazil.Design/methodology/approachThe method adopted is a multiple-case study. The authors conducted face-to-face interviews with six ceramic producers and a trade association. The authors also conducted eight interviews with specialists in the ceramic sector. They are two university professors, two class association representatives, one professional from a technological institute, two representatives of the inspection body and one supplier.FindingsCompanies perform on a compliance basis, manifesting a less proactive position when the subject is CSR. In addition, the CSR practices established by the companies are preferably aimed at reducing costs and risks and increasing reputation. Practices in terms of eco-design are linked to lowering costs and growing reputation, as seen from the reduction in the use of inputs and improvements in packaging to minimize costs. These practices improve the company’s reputation from the consumers' perspective. Environmental aspects are dominant due to the legislation.Originality/valueThis paper contributes theoretically since it identifies, from the companies' perspective, the CSR practices related to risk, cost reduction and reputation increase. There are also opportunities to be explored for facilitating their efforts towards a sustainable business model, especially considering the social dimension. From a managerial perspective, this paper provides some input for implementing CSR actions, mainly regarding their implications for society and local communities.
From workplace to home: consequences of supervisor bullying in the hospitality setting
Authors: Selem Kareem M. , Shoukat Muhammad Haroon , Shehata Ali Elsayed , Ahmad Muhammad Shakil , Gursoy Dogan 
Published: January 2024

Purpose This paper highlights the effects of supervisor bullying (SBL) on work–family conflict (WFC), employee voice behavior (EVB), working compulsively (WCO) and working excessively (WEX), as well as the effects of WFC, EVB and WEX on employees' sleeping problems.Design/methodology/approach Data were gathered from 473 five-star hotel employees, and their responses were analyzed using AMOS v.23.Findings SBL significantly lowers EVB while significantly increasing WFC. SBL increases WEX and WCO levels, which may be considered a short-term positive outcome of SBL.Originality/value This paper will help improve understanding of employee reactions to an emotionally charged workplace occurrence.