bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing the surgeon-machine interface: using a novel instance-segmentation framework for intraoperative landmark labelling
Authors: Park Jay J. , Doiphode Nehal , Zhang Xiao , Pan Lishuo , Blue Rachel , Shi Jianbo , Buch Vivek P. 
Published: October 2023

IntroductionThe utilisation of artificial intelligence (AI) augments intraoperative safety, surgical training, and patient outcomes. We introduce the term Surgeon-Machine Interface (SMI) to describe this innovative intersection between surgeons and machine inference. A custom deep computer vision (CV) architecture within a sparse labelling paradigm was developed, specifically tailored to conceptualise the SMI. This platform demonstrates the ability to perform instance segmentation on anatomical landmarks and tools from a single open spinal dural arteriovenous fistula (dAVF) surgery video dataset.MethodsOur custom deep convolutional neural network was based on SOLOv2 architecture for precise, instance-level segmentation of surgical video data. Test video consisted of 8520 frames, with sparse labelling of only 133 frames annotated for training. Accuracy and inference time, assessed using F1-score and mean Average Precision (mAP), were compared against current state-of-the-art architectures on a separate test set of 85 additionally annotated frames.ResultsOur SMI demonstrated superior accuracy and computing speed compared to these frameworks. The F1-score and mAP achieved by our platform were 17% and 15.2% respectively, surpassing MaskRCNN (15.2%, 13.9%), YOLOv3 (5.4%, 11.9%), and SOLOv2 (3.1%, 10.4%). Considering detections that exceeded the Intersection over Union threshold of 50%, our platform achieved an impressive F1-score of 44.2% and mAP of 46.3%, outperforming MaskRCNN (41.3%, 43.5%), YOLOv3 (15%, 34.1%), and SOLOv2 (9%, 32.3%). Our platform demonstrated the fastest inference time (88ms), compared to MaskRCNN (90ms), SOLOV2 (100ms), and YOLOv3 (106ms). Finally, the minimal amount of training set demonstrated a good generalisation performance –our architecture successfully identified objects in a frame that were not included in the training or validation frames, indicating its ability to handle out-of-domain scenarios.DiscussionWe present our development of an innovative intraoperative SMI to demonstrate the future promise of advanced CV in the surgical domain. Through successful implementation in a microscopic dAVF surgery, our framework demonstrates superior performance over current state-of-the-art segmentation architectures in intraoperative landmark guidance with high sample efficiency, representing the most advanced AI-enabled surgical inference platform to date. Our future goals include transfer learning paradigms for scaling to additional surgery types, addressing clinical and technical limitations for performing real-time decoding, and ultimate enablement of a real-time neurosurgical guidance platform.
Design of a multi-epitope vaccine against brucellosis fused to IgG-fc by an immunoinformatics approach
Authors: Wu Aodi , Wang Yueli , Ali Adnan , Xu Zhenyu , Zhang Dongsheng , Zhumanov Kairat , Sheng Jinliang , Yi Jihai 
Published: October 2023

IntroductionBrucella, a type of intracellular Gram-negative bacterium, has unique features and acts as a zoonotic pathogen. It can lead to abortion and infertility in animals. Eliminating brucellosis becomes very challenging once it spreads among both humans and animals, putting a heavy burden on livestock and people worldwide. Given the increasing spread of brucellosis, it is crucial to develop improved vaccines for susceptible animals to reduce the disease’s impact.MethodsIn this study, we effectively used an immunoinformatics approach with advanced computer software to carefully identify and analyze important antigenic parts of Brucella abortus. Subsequently, we skillfully designed chimeric peptides to enhance the vaccine’s strength and effectiveness. We used computer programs to find four important parts of the Brucella bacteria that our immune system recognizes. Then, we carefully looked for eight parts that are recognized by a type of white blood cell called cytotoxic T cells, six parts recognized by T helper cells, and four parts recognized by B cells. We connected these parts together using a special link, creating a strong new vaccine. To make the vaccine even better, we added some extra parts called molecular adjuvants. These included something called human β-defensins 3 (hBD-3) that we found in a database, and another part that helps the immune system called PADRE. We attached these extra parts to the beginning of the vaccine. In a new and clever way, we made the vaccine even stronger by attaching a part from a mouse’s immune system to the end of it. This created a new kind of vaccine called MEV-Fc. We used advanced computer methods to study how well the MEV-Fc vaccine interacts with certain receptors in the body (TLR-2 and TLR-4).ResultsIn the end, Immunosimulation predictions showed that the MEV-Fc vaccine can make the immune system respond strongly, both in terms of cells and antibodies.DiscussionIn summary, our results provide novel insights for the development of Brucella vaccines. Although further laboratory experiments are required to assess its protective effect.
Ground Fire Legacy Effects on Water-Dynamics of Volcanic Tropical Soils
Authors: Olivares-Martinez Luis D. , Gomez-Tagle Alberto , Mataix-Solera Jorge 
Published: October 2023

The forest floor is a critical component in maintaining the life cycles of forest ecosystems. It normally includes organic soil horizons, known as duff and litter, which are prone to be rapidly consumed after flaming and smoldering fires. This work aims to understand the legacy effects of surface and ground fires on the infiltration capacity of a volcanic forest soil. We studied five sites with fires recorded in the last 20 years. All of them are located in pine-oak forests of the volcanic mountain region in central Mexico with a temperate climate and Andic soil properties. Tension-infiltration tests were carried out to determine hydraulic conductivity and the number of active macropores. After each test, cores were taken to evaluate in a laboratory setting, where soil water repellency at different moisture concentrations and the integrative dynamic repellency index were determined. Field-saturated hydraulic conductivity was moderately high in all sites, with mean values of 13 and 42 mm·h−1 for burned and control plots, respectively. A non-linear relationship was found between recurrence and type of fires with the concentration of active pores and several dynamic water repellency parameters. This work confirmed the presence of latent combustion in these temperate neotropical forests. The changes in soil water repellency and hydraulic conductivity detected do not necessarily imply an exceeded soil infiltration capacity. However, many of the fires in this region are associated with increasing agricultural activities, so further studies are needed to determine if higher fire frequencies could exceed the resilience capacity of the soils triggering land degradation.
Co-Inoculation with Bradyrhizobium and Humic Substances Combined with Herbaspirillum seropedicae Promotes Soybean Vegetative Growth and Nodulation
Authors: Canellas Luciano Pasqualoto , Silva Rakiely Martins , Barbosa Lucas José da Silva , Sales Fernando Soares , Ribeiro Rafael Chaves , Mota Gabriela Petroceli , Olivares Fábio Lopes 
Published: October 2023

The effect of humic substances (HSs) in combination with or without plant-growth-promoting bacteria on soybean vegetative growth and root nodulation was examined in this study. Seeds were inoculated with Bradyrhizobium japonicum SEMIA 5079 in the presence of HSs from leonardite and Herbaspirillum seropedicae HRC54. Additional HSs and H. seropedicae application at the substrate surface was conducted at the V3 stage. The experiment was carried out in a greenhouse using pots filled with a top layer of an Oxisol soil, and plants were harvested at the R1 stage. The HS and H. seropedicae treatments significantly promoted plant shoot and root growth. The number and weight of soybean nodules were higher in the treated plants when compared to a control. The plant nodulation process was affected by the treatments that included activities of malate dehydrogenase (MDH), nitrate reductase (NR) and plasma membrane H+-ATPase (MHA). At low concentrations, the HSs and H. seropedicae increased the nodule quantity, size and weight, favoring plant growth. Combining humic substances and plant-growth-promoting bacteria (PGPB) could be a promising approach to promoting soybean nodulation and increasing crop production.
Study on the Nonlinear Permeability Mechanism and Pore Structure Characteristics of Deep Confined Aquifers
Authors: Peng Shilong , Li Zhijun , Xu Yuhao , Cao Guangyong 
Published: October 2023

The study of deep soil mechanics is the basis of deep shaft construction. Exploring the nonlinear permeability mechanism of deep confined aquifers in depth is the prerequisite and foundation for carrying out calculations of the hydrophobic consolidation settlement of thick alluviums and preventing and controlling deep-well-damage disasters. Against the background of shaft damage caused by hydrophobic consolidation settlement of the bottom aquifer of thick alluviums, a joint HPLTC-HPPNP (high-pressure long-term consolidation and high-pore-pressure nonlinear permeability) test was carried out on the bottom aquifer of thick alluviums based on the ETAS test system. This paper studied the evolution law of the permeability coefficient (kv) of bottom aquifers under different heads of confined water, confining pressures (σr), permeability hydraulic gradients (i) and loading–unloading methods. The internal pore structure characteristics of clayey sand were obtained by using low-field nuclear magnetic resonance (NMR) technology to explore the clayey sand’s nonlinear permeability micro-mechanism. The research results showed that the bottom aquifer seepage volume (ΔQi) under high stress is affected by the head pressure difference and pore water dissipation, and kv decreases with an increasing σr according to the power function relationship. The influence of the hydraulic gradient (i) on kv is significantly influenced by σr. When σr < 4 MPa, kv decreased with an increasing i, and when σr > 4 MPa, kv increased with an increasing i first, then decreased, before then tending to be stable. Under different stress states, the T2 spectrum of clayey sand showed a bispectrum peak type, and the adsorbed water content decreased linearly with an increasing σr, while the capillary water decreased according to the power function. The content of capillary water in the permeable pores plays a key role in the permeability of clayey sand, and it has a power function relationship with σr. The research results of this paper provide a good experimental method for the study of deep soil permeability characteristics and parameter determination, provide a theoretical basis for deep alluvial hydrophobic consolidation and settlement, and further make up for the shortcomings of existing deep soil mechanics in permeability characteristics.
Mitogenomic Analysis of Pterioidea (Bivalvia: Pteriomorphia): Insights into the Evolution of the Gene Rearrangements
Authors: Zhang Yu , Qi Lu , Li Fengping , Yang Yi , Gu Zhifeng , Liu Chunsheng , Li Qi , Wang Aimin 
Published: October 2023

The complete mitogenomes of Pinctada albina and Pinctada margaritifera were sequenced in this study, with sizes of 23,841 bp and 15,556 bp, respectively. The mitochondrial genome analysis of eight Pterioidea species indicated the existence of gene rearrangements within the superfamily. The ATP8 gene was not detected in the two new mitogenomes, and rrnS was found to be duplicated in P. albina’s mitogenome. The reconstructed phylogeny based on mitogenomes strongly supported the monophyly of Pterioidea and provided robust statistical evidence of the phylogenetic relationships within Pteriomorphia. The analysis of the mitochondrial gene order revealed that of P. margaritifera to be the same as the ancestral order of Pterioidea. The gene orders of the Pterioidea species were mapped to the phylogenetic tree, and the gene rearrangement events were inferred. These results provide important insights that will support future research, such as studies extending the evolutionary patterns of the gene order from P. margaritifera to other species and determining the evolutionary status of Pterioidea within the infraclass Pteriomorphia.
Green Extraction of Antioxidant Fractions from Humulus lupulus Varieties and Microparticle Production via Spray-Drying
Authors: Ferreira-Anta Tania , Torres María Dolores , Vilarino Jose Manuel , Dominguez Herminia , Flórez-Fernández Noelia 
Published: October 2023

The formulation of polymeric microparticles to encapsulate bioactive compounds from two hop varieties (Nugget and Perle) using sequential green extraction processes was performed. The technologies used were ultrasound-assisted extraction (UAE) and pressurized hot water (PHW) extraction. Liquid phases were analyzed for total phenolic content (~2%), antioxidant activity (IC50, DPPH: 3.68 (Nugget); 4.46 (Perle) g/L, TEAC (~4–5%), FRAP (~2–3%), and reducing power (~4%)), protein content (~1%), oligosaccharide content (~45%), and for structural features. The fractions obtained from UAE were selected to continue with the drying process, achieving the maximum yield at 120 °C (Perle) and 130 °C (Nugget) (~77%). Based on these results, the formulation of polymeric microparticles using mannitol as the carrier was performed with these fractions. The production yield (~65%), particle size distribution (Perle: 250–750 µm and Nugget: ~100 µm), and rheological features (30–70 mPa s at 0.1 s−1) were the parameters evaluated. The UAE extracts from hop samples processed using a sustainable aqueous treatment allowed the formulation of microparticles with a suitable yield, and morphological and viscosity properties adequate for potential food and non-food applications.
Suplementação de fontes de oleuropeína em doenças neurodegenerativas: revisão sistemática
Authors: Pinheiro Faleiro Giovana , Borges Matheus Büttner , Pureza Isabele Rejane de Oliveira Maranhão , Dal Bosco Simone Morelo 
Published: October 2023

Introdução. Os frutos e folhas extraídos das Oliveiras, como o azeite de oliva extra virgem (AOEV) e seu polifenol mais abundante, a Oleuropeína (OLE), apresentam diversos benefícios terapêuticos, incluindo atividade neuroprotetora. A população idosa enfrenta a ameaça de problemas de saúde relacionados à neurodegeneração. Biomarcadores de alterações cerebrais de Alzheimer, além de sintomas como declínio cognitivo de algumas funções, perda de memória ou velocidade de processamento são sintomas que podem indicar o desenvolvimento de doenças neurodegenerativas exigindo que rotas de tratamento eficazes sejam procuradas. Objetivo. O objetivo desta revisão foi avaliar os efeitos da suplementação de azeite de oliva e outras fontes de oleuropeína em indivíduos com doenças neurodegenerativas. Método. Por meio dos bancos de dados PubMed, Scopus e Web of Science, estudos clínicos que examinam a relação entre AOEV, Oleuropeína e doenças neurodegenerativas até janeiro de 2023 foram sistematicamente pesquisados. A análise de evidências foi realizada usando ferramentas robustas para avaliar o risco de viés (RoB 2.0) e qualidade metodológica (GRADE) dos estudos incluídos. Resultados. Um total de quatro estudos foram incluídos. As condições neurológicas incluem Doença de Alzheimer e Comprometimento Cognitivo Leve. Conclusão. No geral, benefícios terapêuticos neuroprotetores foram observados através da suplementação de AOEV e OLE. Os estudos atualmente publicados, no entanto, são limitados, exigindo continuidade e aperfeiçoamento da metodologia de intervenção empregada.
HUBUNGAN KECERDASAN EMOSIONAL PERAWAT PELAKSANA DENGAN PELAYANAN ASUHAN KEPERAWATAN PASIEN DI INSTALASI RAWAT INAP RUMAH SAKIT JUANDA KUNINGAN TAHUN 2023
Authors: Puspanegara Aditiya , Nabila Nadiatul Syifa , Rusmianingsih Nining 
Published: October 2023

Kecerdasan emosional merupakan suatu kepiawaian dalam hal menstimulasi diri, bersikukuh terhadap frustasi, membenahi kondisi hati, berfikir jernih dan berempati sehingga bisa memberikan pelayanan asuhan keperawatan yang berkualitas. Tujuan penelitian ini untuk menganalisis hubungan kecerdasan emosional perawat pelaksana dengan pelayanan asuhan keperawatan pasien di instalasi rawat inap Rumah Sakit Juanda Kuningan tahun 2023.
Jenis penelitian ini adalah penelitian kuantitatif dengan pendekatan cross sectional. Populasi dalam penelitian ini 64 responden dengan jumlah sampel sebanyak 54 responden dengan menggunakan total sampling. Instrumen penelitian ini menggunakan kuesioner dan penatalaksanaannya menggunakan uji statistik Rank Spearman. Hasil univariat diperoleh kecerdasan emosional perawat pelaksana dengan kategori tinggi (27,8%) dan kategori rendah (13%), sedangkan pelayanan asuhan keperawatan pasien dengan kategori baik (31,5%) dan kategori kurang (20,4%). Analisis bivariat menunjukan ada hubungan kecerdasan emosional perawat pelaksana dengan pelayanan asuhan keperawatan pasien dengan didapatkan nilai p 0,007 < 0,05 dan kekuatan korelasi (r) 0,361.
Terdapat hubungan antara kecerdasan emosional perawat pelaksana dengan pelayanan asuhan keperawatan. Disarankan pihak Rumah Sakit meningkatkan mutu pelayanan khususnya pelayanan asuhan keperawatan yang sesuai dengan harapan masyarakat.
HUBUNGAN ANTARA DUKUNGAN KELUARGA DAN FUNGSI KOGNITIF TERHADAP KEMAMPUAN INTERAKSI SOSIAL LANSIA
Authors: Hermansyah Heri , Rahmawati Fadiah 
Published: October 2023

Menghabiskan waktu bersama orang lain sangat penting untuk kesejahteraan emosional dan mental lansia. Hubungan keluarga dan fungsional kognitif diketahui merupakan faktor penting yang dapat mendukung interaksi sosial di kalangan lansia. Namun, bagaimana dampak hubungan dukungan keluarga dan fungsi kognitif terhadap kemapuan lansia dalam berinteraksi sosial masih belum diketahui dengan jelas. Oleh karenanya, penelitian ini bertujuan untuk menganalisis hubungan antara dukungan keluarga dan fungsi kognitif terhadap kempuan interaksi sosial lansia.
Penelitian ini menggunakan metode cross-sectional dengan besar sampel sebanyak 59 orang berusia rata-rata 60 tahun yang terdaftar sebagai penduduk Desa Jadimulya Kecamatan Gunung Jati Cirebon yang diambil dengan tehnik stratified random sampling. Data dikumpulkan dengan menggunakan kuesioner dan dianalisis dengan uji korelasi Rank Spearman Test. Adapun hasil penelitian ini menunjukan (1) ada hubungan antara dukungan sosial keluarga terhadap kemampuan interaksi sosial pada lansia dengan nilai p-value = 0,000 < 0,05 dan rh0 = 0,547; (2) ada hubungan antara fungsi kognitif terhadap kemampuan interaksi sosial pada lansia dengan nilai p-value = 0,000 < 0,05 dan rh0 = 0,478.
HUBUNGAN PERAN PENGAWAS MENELAN OBAT (PMO) DAN DUKUNGAN KELUARGA DENGAN KEPATUHAN MINUM OBAT PADA PENDERITA TUBERCULOSIS DI PUSKESMAS CIAWIGEBANG KABUPATEN KUNINGAN 2023
Authors: Mulyati Lia , Hendriana Yana , Sari Ade Mulya 
Published: October 2023

Latar Belakang : Indonesia menduduki peringkat kedua untuk angka kesakitan TBC terbanyak di dunia setelah India. Kepatuhan pasien TBC dalam meminum obat menjadi dasar kesembuhan sehingga di perlukan pengawasan langsung oleh Pengawas Menelan Obat (PMO) yang melibatkan keluarga. Penelitian ini bertujuan mengetahui hubungan antara Peran Pemantauan Minum Obat (PMO) Dan Dukungan Keluarga Dengan Kepatuhan Minum Obat Pada PenderitaTuberculosis Di Puskesmas Ciawigebang Kabupaten Kuningan.
Metode : Jenis penelitian adalah penelitian Kuantitatif. Rancangan penelitian ini adalah cross sectional. Pengambilan sampel menggunakan teknik probability sampling sebanyak 93 responden. Instrumen menggunakan kuesioner. Analisis univariat menggunakan distribusi frekuensi dan bivariat menggunakan Chi Square.
Hasil : Hasil menujukkan sebagian besar peran PMO sudah melakukan tugas scara optimal sebanyak 86 (92,5%), sebagian besar responden medapat dukungan dari keluarga dalam mengkonsumsi obat TBC sebanyak 57 responden (61,3%) dan patuh minum OAT sebanyak 89 responden (95,7%). Analisis bivariat diperoleh peran PMO (p = 0,027) dan dukungan keluarga (p = 0,020) dengan CI = 95%.
Simpulan : Peran PMO dan dukungan keluarga memiliki hubungan yang signifikan dengan kepatuhan minum OAT. Disarankan petugas PMO diharapkan melakukan pemberdayaan keluarga dan kader PMO untuk meningkatkan kepatuhan minum OAT pada pasien TBC.
PENGARUH TERAPI RENDAM KAKI DENGAN AIR REBUSAN JAHE DAN SERAI TERHADAP PENURUNAN TEKANAN DARAH PADA LANSIA HIPERTENSI DI DESA PANINGGARAN KECAMATAN DARMA KABUPATEN KUNINGAN TAHUN 2023
Authors: Hendriana Yana , Septiadi Dikri , Nugraha Moch. Didik 
Published: October 2023

Latar Belakang : Di Indonesia, prevalensi hipertensi berdasarkan Riskesdas 2018 menunjukkan bahwa dari 25,8% orang menjadi 34,1% yang menderita hipertensi. Menurut Data Dinas Kesehatan Povinsi Jawa Barat pada tahun 2021 total jumlah penderita hipertensi di Jawa Barat yaitu sebanyak 4.607.116 orang. Di kabupaten Kuningan jumlah hipertensi pada lansia pada tahun 2022 sebanyak 43.632 kasus. Tujuan penelitian ini yaitu untuk menurunkan tekanan darah pada lansia hipertensi. Metode penelitian : Jenis penelitian yang digunakan pada penelitian ini adalah penelitian eksperimesn quasi. Pupulasi lansia yang menderita hipertensi di desa Paninggaran menurut data puskesmas sebanyak 282 orang. Responden dalam penelitian ini berjumlah 17 orang. Teknik sampling dalam penelitian ini menggunakan Teknik pengambilan sampel purvosive sampling dengan menggunakan rumus Federer. Analisis uji statistik menggunakan uji statistic Wilcoxon. Instrument penelitian menggunakan lembar observasi dan SOP terapi rendam kaki dengan air rebusan jahe dan serai. Hasil : Hasil peneleitian menunjukan rata-rata tekanan darah sebelum dilakukan intervensi adalah 146.47/95.29 mmHg. Setelah dilakukan terapi rendam kaki dengan air rebusan jahe dan serai rata-rata tekanan darah 130.59/85.29 mmHg. Hasil analisis bivariat didapatkan p value = ,000. Simpulan : Terdapat pengaruh terapi rendam kaki dengan air rebusan jahe dan serai terhadap penurunan tekanan darah pada lansia hipertensi di desa paninggaran tahun 2023. Saran : Peneliti menyarankan pada kader Posbindu melakukan terapi rendam kaki dengan air rebusan jahe dan serai guna menurunkan tekanan darah pada lansia.
HUBUNGAN ANTARA PENGETAHUAN DAN SIKAP PERAWAT TENTANG VISI DAN MISI RUMAH SAKIT DENGAN KEPUASAN KERJA PERAWAT DI RSU KMC KUNINGAN TAHUN 2022
Authors: Pranatha Aria , Moonti Mutia Agustiani , Gumelar Panzi 
Published: October 2023

Latar Belakang: Perawat memegang peran penting dalam meningkatkan kualitas pelayanan demi kepuasan pasien tetapi perawat kurang dapat menjalankan perannya karena kurang memiliki pengetahuan dan sikap sebagai faktor yang sangat penting dalam menentukan kepuasan kerja perawat. Tujuan dari penilitian ini adalah untuk mengetahui hubungan antara pengetahuan dan sikap perawat tentang visi dan misi rumah sakit dengan kepuasan kerja perawat di RSU KMC Kuningan Tahun 2022.
Metode: Jenis penelitian ini adalah analitik korelasi dengan desain cross sectional dan teknik pengambilan sampel adalah teknik total sampling didapatkan jumlah 42 responden. Analisis statistik dilakukan secara univariat dan bivariat dengan metode Rank Spearman.
Hasil: Hasil penelitian sebagian besar responden yang memiliki pengetahuan baik sebanyak 18 responden (42,9%), yang memiliki sikap positif sebanyak 33 reponden (78,6%), yang memliki kepuasan kerja puas sebanyak 32 responden (76,2%).
Kesimpulan: Berdasarkan hasil penelitian maka, terdapat hubungan yang signifikan antara pengetahuan perawat tentang visi dan misi rumah sakit dengan kepuasan kerja perawat di RSU KMC Kuningan Tahun 2022 dengan p value = 0,002.dan terdapat hubungan yang signifikan antara sikap perawat tentang visi dan misi rumah sakit dengan kepuasan kerja perawat di RSU KMC Kuningan Tahun 2022 dengan p value = 0,000.
Saran: Saran diharapkan pengetahuan dan sikap perawat tentang visi dan misi rumah sakit dapat meningkatkan kepuasan kerja perawat dalam pelayanan asuhan keperawatan.