bluuebunny/crossref_metadata_2025_split huggingface dataset:
METTL3 regulates autophagy of hypoxia-induced cardiomyocytes by targeting ATG7
Authors: Li Linnan , Cheng Hao , Zhou Yufei , Zhao Di , Zhang Xiaoxue , Wang Yajun , Ma Jianying , Ge Junbo 
Published: February 2025

Abstract
N6-methyladenosine (m6A) mRNA modification is the most common mRNA internal modification in eukaryotes, which participates in a variety of biological processes. However, the role of m6A methylation in regulating autophagy induced by ischemia and hypoxia remains to be widely investigated. Here, we investigated the impact of METTL3, a key m6A methyltransferase, on the autophagy regulation in ischemic and hypoxic cardiomyocytes, as well as in mice following acute myocardial infarction (AMI). METTL3 negatively regulated autophagy in cardiomyocytes under ischemia and hypoxia conditions. Silencing METTL3 enhanced autophagy and mitigated cardiomyocyte injury, whereas overexpression of METTL3 exerted the opposite effect. Mechanistically, METTL3 methylated ATG7 mRNA, a crucial autophagy-related gene, leads to the recruitment of the m6A-binding protein YTHDF2. Subsequently, YTHDF2 facilitated the degradation of ATG7 mRNA, consequently inhibiting autophagy and exacerbating cellular damage. Our study shed light on the pivotal role of METTL3-mediated m6A modification in the regulation of autophagy during AMI, providing novel insights into the functional significance of m6A methylation and its regulatory mechanisms.
The ubiquitin ligase Pellino1 targets STAT3 to regulate macrophage-mediated inflammation and tumor development
Authors: Hwang Soeun , Park Junhee , Koo Seo-Young , Lee Si-Yeon , Jo Yunju , Ryu Dongryeol , Go Heounjeong , Lee Chang-Woo 
Published: February 2025

Abstract
Receptor-mediated signaling could be modulated by ubiquitination of pathway intermediates, but the role of such modification in the pathogenesis of inflammation and inflammation-related cancer is lesser known. The ubiquitin ligase Pellino1 has been shown to modulate immune signals by enabling various immune cells to respond to their receptor signals effectively. Here, we show that Pellino1 levels are elevated in patients with colitis, patients with colitis-associated colon cancer (CAC), and murine models of these conditions. In a monocyte-specific Pellino1 knock-out mouse model, we find reduced macrophage migration and activation, leading to attenuated development of colitis and CAC in male mice. Mechanistically, Pellino1 targets STAT3 for lysine 63-mediated ubiquitination, resulting in pathogenic activation of STAT3 signaling. Taken together, our findings reveal a macrophage-specific ubiquitination signaling axis in colitis and CAC development and suggest that Pellino1 is a potential candidate for treating chronic inflammation and inflammation-related cancer.
Tailored topotactic chemistry unlocks heterostructures of magnetic intercalation compounds
Authors: Husremović Samra , Gonzalez Oscar , Goodge Berit H. , Xie Lilia S. , Kong Zhizhi , Zhang Wanlin , Ryu Sae Hee , Ribet Stephanie M. , Fender Shannon S. , Bustillo Karen C. , Song Chengyu , Ciston Jim , Taniguchi Takashi , Watanabe Kenji , Ophus Colin , Jozwiak Chris , Bostwick Aaron , Rotenberg Eli , Bediako D. Kwabena 
Published: January 2025

Abstract
The construction of thin film heterostructures has been a widely successful archetype for fabricating materials with emergent physical properties. This strategy is of particular importance for the design of multilayer magnetic architectures in which direct interfacial spin-spin interactions between magnetic phases in dissimilar layers lead to emergent and controllable magnetic behavior. However, crystallographic incommensurability and atomic-scale interfacial disorder can severely limit the types of materials amenable to this strategy, as well as the performance of these systems. Here, we demonstrate a method for synthesizing heterostructures comprising magnetic intercalation compounds of transition metal dichalcogenides (TMDs), through directed topotactic reaction of the TMD with a metal oxide. The mechanism of the intercalation reaction enables thermally initiated intercalation of the TMD from lithographically patterned oxide films, giving access to a family of multi-component magnetic architectures through the combination of deterministic van der Waals assembly and directed intercalation chemistry.
Versatile nitrate-respiring heterotrophs are previously concealed contributors to sulfur cycle
Authors: Shao Bo , Xie Yuan-Guo , Zhang Long , Ruan Yang , Liang Bin , Zhang Ruochen , Xu Xijun , Wang Wei , Lin Zhengda , Pei Xuanyuan , Wang Xueting , Zhao Lei , Zhou Xu , Wu Xiaohui , Xing Defeng , Wang Aijie , Lee Duu-Jong , Ren Nanqi , Canfield Donald E. , Hedlund Brian P. , Hua Zheng-Shuang , Chen Chuan 
Published: January 2025

Abstract
Heterotrophic denitrifiers play crucial roles in global carbon and nitrogen cycling. However, their inability to oxidize sulfide renders them vulnerable to this toxic molecule, which inhibits the key enzymatic reaction responsible for reducing nitrous oxide (N2O), thereby raising greenhouse gas emissions. Here, we applied microcosm incubations, community-isotope-corrected DNA stable-isotope probing, and metagenomics to characterize a cohort of heterotrophic denitrifiers in estuarine sediments that thrive by coupling sulfur oxidation with denitrification through chemolithoheterotrophic metabolism. Remarkably, ecophysiology experiments from enrichments demonstrate that such heterotrophs expedite denitrification with sulfur acting as alternative electron sources and substantially curtail N2O emissions in both organic-rich and organic-limited environments. Their flexible, non-sulfur-dependent physiology may confer competitive advantages over conventional heterotrophic denitrifiers in detoxifying sulfide, adapting to organic matter fluctuations, and mitigating greenhouse gas emissions. Our study provides insights into the ecological role of heterotrophic denitrifiers in microbial communities with implications for sulfur cycling and climate change.
Nucleus pulposus cell network modelling in the intervertebral disc
Authors: Tseranidou Sofia , Segarra-Queralt Maria , Chemorion Francis Kiptengwer , Le Maitre Christine Lyn , Piñero Janet , Noailly Jérôme 
Published: January 2025

Abstract
Intervertebral disc degeneration (IDD) results from an imbalance between anabolic and catabolic processes in the extracellular matrix (ECM). Due to complex biochemical interactions, a comprehensive understanding is needed. This study presents a regulatory network model (RNM) for nucleus pulposus cells (NPC), representing normal intervertebral disc (IVD) conditions. The RNM includes 33 proteins, and 153 interactions based on literature, incorporating key NPC regulatory mechanisms. A semi-quantitative approach calculates the basal steady state, accurately reflecting normal NPC activity. Model validation through published studies replicated pro-catabolic and pro-anabolic shifts, emphasizing the roles of transforming growth factor beta (TGF-β) and interleukin-1 receptor antagonist (IL-1Ra) in ECM regulation. This IVD RNM is a valuable tool for predicting IDD progression, offering insights into ECM degradation mechanisms and guiding experimental research on IVD health and degeneration.
TRα1 mutant suppresses KLF9 to cause endometrial metaplasia with ectopic IL-33 expression leading to uterine fibrosis and infertility
Authors: Edmondson Elijah , Kimura Takahito , Hwang Eunmi , Kim Minjun , Warner Andrew , Zhu Yuelin , Zhao Li , Yu Yanlin , Zhu Xuguang , Hernandez Maria , Kedei Noemi , Cheng Sheue-yann 
Published: January 2025

Abstract
Thyroid hormone receptors (TRs) mediate the genomic actions of thyroid hormone. Mutations of THRA gene cause a human disease known as resistance to thyroid hormone (RTHα). We created a mouse model expressing a dominant negative mutated TRα1 (Thra1PV/+ mice) that exhibits growth retardation, bone abnormalities, constipation, and anemia, as found in RTHα patients. In addition, female Thra1PV/+ mice exhibit decreased fertility. In the present study, we aimed to characterize the molecular events leading to infertility. Histologically, there was progressive uterine atrophy in Thra1PV/+ mutant mice, characterized by squamous metaplasia of the endometrial mucosa and endometrial fibrosis. RNA-seq analysis of laser-captured micro-dissected endometrium and spatial transcriptomics revealed a key role for Krüppel-like factor (Klf9), a directly-regulated TR target gene, in normal endometrial differentiation. Klf9 was suppressed in the endometrium of mice harboring mutated TRα1 and pathway analysis revealed that deficient Klf9 signaling was associated with squamous differentiation, consistent with the endometrial metaplasia observed histologically. Further, we showed that this metaplastic endometrial mucosa was the source of ectopic IL-33, which was associated with increased T-cell infiltrates, destruction of glands, and endometrial fibrosis. Our studies provide new insights to understand uterine epithelial morphogenesis and how thyroid dysfunction could lead to female infertility.
Real-time discrimination of earthquake signals by integrating artificial intelligence technology into IoT devices
Authors: Geng Zhi , Wang Yanfei , Pan Wenyong , Yu Caixia , Bai Zhijing , Zhang Hongzhou 
Published: January 2025

Abstract
The real-time detection and analysis of seismic signals is crucial in geophysics research, especially when it comes to monitoring catastrophic events. We present an evolutionary deep learning method that yields a model named MCU-Quake. This model encodes the discrimination process as a single numerical value, offering interpretability with only 2693 parameters. Trained on raw seismic waveforms from Utah, USA, MCU-Quake demonstrates its generalization capability across a global natural earthquake dataset. Notably, the model effectively identifies typical explosions during the Russia-Ukraine war in Europe. The knowledge to discriminate between ambient noise, explosions and natural earthquakes can be represented by values of −5.01 (std: 1.14), 1.96 (std: 0.36), 1.01 (std: 0.49), respectively. The model can be deployed on Internet of Things (IoT) devices, including most microcontrollers, which are constrained by limited computational resources (kilo-bytes of memory) and energy consumption (micro-Watts). The results indicate the prospect of on-site missions of artificial intelligent sensors.
Self-trapped exciton luminescence of tellurium doped zero-dimensional tetraethyl tin chloride for optical thermometry
Authors: Chen Chen , Yu Hailong , Qi Hongbo , Dong Gaoke , Li Jing , Yang Gang , Wu Wenzhi 
Published: February 2025

Te4+ doped (TEA)2SnCl6 MCs were synthisized by solvothernal method for the first time, of which structure was obtained by crystal/DFT. Maximium absolute/relative temperature sensitivities reach 0.57% K−1 and 1 × 10−2 K−1 for optical thermometry.
Advancing skin cancer detection integrating a novel unsupervised classification and enhanced imaging techniques
Authors: Rahman Md. Abdur , Fahad Nur Mohammad , Raiaan Mohaimenul Azam Khan , Jonkman Mirjam , De Boer Friso , Azam Sami 
Published: February 2025

AbstractSkin cancer, a severe health threat, can spread rapidly if undetected. Therefore, early detection can lead to an advanced and efficient diagnosis, thus reducing mortality. Unsupervised classification techniques analyse extensive skin image datasets, identifying patterns and anomalies without prior labelling, facilitating early detection and effective diagnosis and potentially saving lives. In this study, the authors aim to explore the potential of unsupervised learning methods in classifying different types of skin lesions in dermatoscopic images. The authors aim to bridge the gap in dermatological research by introducing innovative techniques that enhance image quality and improve feature extraction. To achieve this, enhanced super‐resolution generative adversarial networks (ESRGAN) was fine‐tuned to strengthen the resolution of skin lesion images, making critical features more visible. The authors extracted histogram features to capture essential colour characteristics and used the Davies–Bouldin index and silhouette score to determine optimal clusters. Fine‐tuned k‐means clustering with Euclidean distance in the histogram feature space achieved 87.77% and 90.5% test accuracies on the ISIC2019 and HAM10000 datasets, respectively. The unsupervised approach effectively categorises skin lesions, indicating that unsupervised learning can significantly advance dermatology by enabling early detection and classification without extensive manual annotation.
Study on optimisation methods for production processes of composite insulator sheath and GRP rod
Authors: Luo Haowen , Zhang Sida , Cheng Li , Li Zhiyi 
Published: February 2025

AbstractWater diffusion significantly contributes to the abnormal heating in composite insulators. At the same time, the material performance shortcomings caused by the composite insulator production process can further increase water diffusion. To optimise the production process of composite insulators to prevent or reduce such abnormal heating, the fillers of the composite insulator sheath and the production of glass‐fibre‐reinforced plastic (GRP) rods are analysed. Based on the water absorption model of the sheath and the fibre infiltration model of the rod, it is identified that the flame retardant particle size is a factor that affects the water absorption of the sheath, whereas the epoxy resin liquid viscosity and the fibre volume fraction are factors that affect the porosity of the GRP rod. The experimental results show that the use of modified large‐particle‐size flame retardants can reduce the water absorption rate of the sheath, whereas low‐viscosity liquids and high‐fibre volume fractions can reduce the porosity of the GRP rod. The sheath and GRP rod produced using the optimised process continue to meet the standards.
Management of Posterior Cervical Epidural Abscess Complicated by Non-Communicating Tetra-Ventricular Hydrocephalus: A Case Report and Review of the Literature
Authors: Boris Gbassara Koulagna , Djoubairou Ben Ousmanou , Figuim Bello , Poulenc Njofang Teto , Mathieu Motah , Vdp Djientcheu 
Published: January 2025

Abstract
Background Spinal epidural abscess (SEA) is an uncommon condition, especially in children. Its occurrence in the cervical spine, particularly without significant osseous involvement, is exceedingly rare. We report a case of non-communicating tetra-ventricular hydrocephalus associated with a posterior cervical epidural abscess. The condition was managed with surgical drainage, specific antibiotics, and ventriculoperitoneal shunting.
Case Description A 15-year-old female presented with a history of posterior cervical abscess drainage. Bacteriological findings revealed two microorganisms (Staphylococcus epidermidis and tuberculosis) despite initial improvement following surgical intervention and antibiotic therapy, the patient experienced a recurrence of symptoms, including fever and deterioration in consciousness. Imaging revealed a tetra ventricular hydrocephalus, prompting urgent ventriculoperitoneal shunt placement. Subsequent treatment with anti-tuberculous therapy and vancomycin led to favorable outcomes.
Conclusion SEA poses diagnostic and management challenges, particularly in resource-limited settings. Prompt recognition, appropriate antimicrobial therapy, and surgical intervention are crucial for favorable outcomes. Consideration of tuberculous and S. epidermidis etiology, even in atypical presentations, is essential.
Comparison of Graft Uptake in Perforated Eardrums with and without Myringosclerosis: A Prospective Case-control Study in a Tertiary Centre
Authors: Rehman Ayaz , Deva Faizah Ashfah Latief , Wani Asef Ahmad , Masoodi Majid , Malik Bashir , Makhdoomi Owais 
Published: February 2025

Abstract
Introduction Various factors have been reported to affect the rates of success after tympanoplasty, among them, myringosclerosis. However, there are few studies focusing only on the effect of myringosclerotic plaque removal on tympanoplasty outcomes.
Objective To compare the outcome of tympanoplasty in perforated eardrums with and without myringosclerosis.
Methods The study included patients aged between 21 and 53 years diagnosed at the outpatient department with inactive mucosal chronic otitis media. The sample was divided into the case group, which included subjects with tympanic membrane perforation and myringosclerosis, and the control group, which included subjects with tympanic membrane perforation only, without myringosclerosis. We assessed the audiological findings of the patients before and after surgery, as well as hearing gain, graft uptake, and residual perforation/reperforation.
Results No significant relationships were observed involving the age or sex of the patient and the closure rate or hearing gain, neither between the location of the perforation and graft uptake. Graft uptake was higher in patients with perforation size < 50%. The graft uptake and hearing gain were higher in the case group.
Conclusion The removal of sclerotic plaques increases the surface of the raw area created by subepithelial excision of the myringosclerotic plaques, leading to a high rate of surgical success for the closure of tympanic membrane defects with coexisting myringosclerosis.
Safety of Abemaciclib in Indian Patients with Hormone Receptor-Positive, Human Epidermal Growth Factor Receptor 2-Negative Locally Advanced and/or Metastatic Breast Cancer: A Multicenter, Nonrandomized, Open-Label, Single-Arm, Phase 4 Study
Authors: Doval Dinesh Chandra , Maksud Tanveer , Nagarkar Rajnish , Thunagappa Satheesh Chiradoni , Taran Rakesh , Prasad Saurabh , Manoharan Anoop T. , Jain Minish , Mehta Dhruv , Mohapatra Prabrajya Narayan , Gupta Sachin , Singh Kritarth Naman , Pruthi Aarohan , Arora Rohit 
Published: February 2025

Background Global phase III trials demonstrated efficacy of abemaciclib in patients with HR +/HER2– metastatic breast cancer (BC) as a first-line therapy in combination with a nonsteroidal aromatase inhibitor (MONARCH-3) or with fulvestrant following progression after endocrine therapy (ET) (MONARCH-2). However, there is limited data on safety and tolerability of abemaciclib plus ET in the metastatic BC setting among Indian patients, which the present study aims to address.
Materials and Methods An open-label, single-arm, phase IV study was conducted across 16 centers in India to assess the safety and tolerability of abemaciclib in patients with HR +/HER2– locally advanced or metastatic BC. Patients were assigned to either cohort A, ET-naive patients (abemaciclib + anastrozole/letrozole) or cohort B, patients progressing after previous ET (abemaciclib + fulvestrant), targeting the same patient population as the global phase III MONARCH-3 and MONARCH-2 trials, respectively. Primary endpoints were all-cause adverse events (AEs) including serious AEs (SAEs).
Statistical Analysis The statistical analysis was conducted using SAS Version 9.4.
Results Two hundred patients were enrolled, with a mean age of 54 years, most (77.0%) were aged ≤ 65 years. The median duration of exposure was similar in both cohorts (cohort A vs. B: 24.3 vs. 24.4 weeks). Overall, 75.5 % of patients reported all-cause AEs, of which 38.5% of the patients reported AEs Common Terminology Criteria for Adverse Events grade 3 and above. The most common grade 3 and above all-cause AEs for abemaciclib were neutropenia (19.0%), followed by anemia (14.0%) and diarrhea (5.5%). Fourteen (7.0%) patients encountered SAEs, including infections (2.0%) and gastrointestinal disorders (1.5%). Most of the patients continued their treatments with appropriate dose reductions (25.5%) and dose omissions (40.5%), and only 2.5% of patients discontinued study treatment due to treatment-related AEs.
Conclusion Abemaciclib in combination with ET was found to have an acceptable tolerability in Indian patients with HR +/HER2– advanced and metastatic BC, consistent with the established safety data as reported in the pivotal global studies. No new clinical safety concerns were identified, with most of the reported AEs and SAEs managed by dose adjustments.
Design and study of valveless piezoelectric pump with streamlined chamber fishtail-type bluff body
Authors: Tian Xiaochao , Wang Zhenming , Yang Kai , Bai Yang , Dai Yingyu , Niu Defeng , Zhong Zhicheng 
Published: January 2025

To enhance the flow rate of a valveless piezoelectric pump and improve the stability of the fluid flow, a valveless piezoelectric pump with a streamlined chamber fishtail-type bluff body (VPFB) is proposed. Compared with the traditional conical chamber, the streamlined chamber reduces the generation of eddy currents and energy loss, increasing the output efficiency of the piezoelectric pump. On the other hand, the fishtail-type bluff body increases the resistance when the fluid flows in the reverse direction, reducing the backflow. First, the three-dimensional model of VPFB is designed and theoretically analyzed based on the piezoelectric inverse effect and fluid dynamics to derive the expression of the relationship affecting the flow rate of the piezoelectric pump. Second, the finite element method is used to analyze the flow field and pressure of VPFB. Finally, the prototype is made, and the experimental platform is built to test the output performance of VPFB. The experimental results show that the output flow rate of VPFB is 27.1 ml/min when the driving voltage is 150 V and the driving frequency is 50 Hz, which is 8% higher compared with the traditional triangular bluff body piezoelectric pump. The fishtail-type bluff body is optimized with parameters, and the maximum output flow rate of the designed piezoelectric pump is 44.18 ml/min when the height is 2 mm. When changing its length to 4 mm, the pump output flow rate can reach 45.51 ml/min. The streamlined chamber proposed in this paper provides a new idea in the research field of valveless piezoelectric pumps.