bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased Cholesterol Synthesis Drives Neurotoxicity in Patient Stem Cell-Derived Model of Multiple Sclerosis
Authors: Ionescu Rosana-Bristena , Nicaise Alexandra M. , Reisz Julie A. , Williams Eleanor C. , Prasad Pranathi , Dzieciatkowska Monika , Stephenson Daniel , Cubero Marta Suarez , Pirvan Liviu , Willis Cory M. , Peruzzotti-Jametti Luca , Fossati Valentina , Edenhofer Frank , Leonardi Tommaso , Frezza Christian , Mohorianu Irina , D’Alessandro Angelo , Pluchino Stefano 
Published: January 2024

SUMMARYSenescent neural progenitor cells have been identified in brain lesions of people with progressive multiple sclerosis (PMS). However, their role in disease pathobiology and contribution to the lesion environment remains unclear.By establishing directly induced neural stem/progenitor cell (iNSC) lines from PMS patient fibroblasts, we studied their senescent phenotypein vitro. Senescence was strongly associated with inflammatory signaling, hypermetabolism, and the senescence associated secretory phenotype (SASP). PMS-derived iNSCs displayed increased glucose-dependent fatty acid and cholesterol synthesis, which resulted in the accumulation of cholesteryl ester-enriched lipid droplets. An HMG-CoA reductase-mediated lipogenic state was found to induce secretion of the SASP in PMS iNSC conditioned media via transcriptional regulation by cholesterol-dependent transcription factors. SASP from PMS iNSCs induced neurotoxicity. Chemical targeting of HMG-CoA reductase using the cholesterol-lowering drug simvastatin (SV) prevented SASP release and resulting neurotoxicity.Our findings suggest a disease-associated, cholesterol-related, hypermetabolic phenotype of PMS iNSCs that leads to neurotoxic signaling and is rescuable pharmacologically.
Heterodimerization of Endolysin Isoforms During Bacterial Infection by Staphylococcal Phage φ2638A
Authors: Zinsli Léa V. , Sobieraj Anna M. , Ernst Patrick , Meile Susanne , Kilcher Samuel , Iseli Cedric , Keller Anja , Dreier Birgit , Mittl Peer R. E. , Plückthun Andreas , Loessner Martin J. , Schmelcher Mathias , Dunne Matthew 
Published: January 2024

AbstractBacteriophage endolysins targeting Gram-positive bacteria typically feature a modular architecture of one or more enzymatically active domains (EADs) and cell wall binding domains (CBDs). Several endolysins also feature internal translational start sites (iTSSs) that produce short variant (SV) isoforms alongside the full-length (FL) endolysin. While the lytic activity of endolysins and their isoforms has been extensively studied as exogenous agents, the purpose behind producing the SV isoform during the phage infection cycle remains to be explored. In this study, we used staphylococcal phage φ2638A as a model to determine the interplay between its full-length endolysin, Ply2638A, and its SV isoform during phage infection. X-ray crystallography structures and AlphaFold-generated models enabled elucidation of individual functions of the M23 endopeptidase, central amidase, and SH3b domains of Ply2638A. Production of the SV isoform (amidase and SH3b) was confirmed during phage infection and shown to form a heterodimer complex with Ply2638A via inter-amidase domain interactions. Using genetically engineered phage variants, we show that production of both isoforms provides an advantage during phage infection as phages producing only one isoform presented impaired lytic activity, which could be partly restored through recombinant protein complementation of the missing isoform. Importantly, when applied as an antimicrobial protein againstStaphylococcus aureusin culture, the activity of Ply2638A remained constant regardless of SV isoform complementation. Drawing from our findings, we propose that SV isoform production provides its biological advantage upon endolysin entry to the periplasmic space to ensure optimal peptidoglycan degradation prior to cell wall lysis and progeny phage release.
Coordinated social interactions are supported by integrated neural representations
Authors: Formica Silvia , Brass Marcel 
Published: January 2024

AbstractJoint actions are defined as coordinated interactions of two or more agents towards a shared goal, often requiring different and complementary individual contributions. However, how humans can successfully act together without the interfering effects of observing incongruent movements is still largely unknown. It has been proposed that interpersonal predictive processes are at play to allow the formation of a Dyadic Motor Plan, encompassing both agents’ shares. Yet, direct empirical support for such an integrated motor plan is still limited. In this study, we aimed at testing the properties of these anticipated representations. We collected EEG data while human participants (N = 36; 27 females) drew shapes simultaneously to a virtual partner, in two social contexts: either they had to synchronize and act jointly, or they performed the movements alongside, but independently. We adopted a multivariate approach to show that the social context influenced how the upcoming action of the partner is anticipated during the interval preceding the movement. We found evidence that acting jointly induces an encoding of the partner’s action that is strongly intertwined with the participant’s action, supporting the hypothesis of an integrative motor plan in joint but not in parallel actions.
In-depth characterisation of a selection of gut commensal bacteria reveals their functional capacities to metabolise dietary carbohydrates with prebiotic potential
Authors: Bedu-Ferrari Cassandre , Biscarrat Paul , Pepke Frederic , Vati Sarah , Chaudemanche Cyril , Castelli Florence , Chollet Céline , Rué Olivier , Hennequet-Antier Christelle , Langella Philippe , Cherbuy Claire 
Published: January 2024

ABSTRACTThe microbial utilisation of dietary carbohydrates is closely linked to the pivotal role of the gut microbiome in human health. Inherent to the modulation of complex microbial communities, a prebiotic implies the selective utilisation of specific substrate, relying on the metabolic capacities of targeted microbes. In this study, we investigated the metabolic capacities of 17 commensal bacteria of the human gut microbiome toward dietary carbohydrates with prebiotic potential. First,in vitroexperiments allowed the classification of bacterial growth and fermentation profiles in response to various carbon sources, including agave inulin, corn fiber, polydextrose and citrus pectin. The influence of phylogenetic affiliation appeared to statistically outweigh carbon sources in determining the degrees of carbohydrate utilisation. Secondly, we narrowed our focus on six commensal bacteria representative of theBacteroidetesandFirmicutesphyla to perform an untargeted HR-LC/MS metabolomic analysis.Bacteroides xylanisolvens,Bacteroides thetaiotaomicron,Bacteroides intestinalis,Subdoligranulum variabile,Roseburia intestinalisandEubacterium rectaleexhibited distinct metabolomic profiles in response to different carbon sources. The relative abundance of bacterial metabolites was significantly influenced by dietary carbohydrates, with these effects being strain-specific and/or carbohydrate-specific. Particularly, the findings indicated an elevation in short-chain fatty acids and other metabolites, including succinate, gamma-aminobutyric acid, and nicotinic acid. These metabolites were associated with putative health benefits. Finally, an RNA-Seq transcriptomic approach provided deeper insights into the underlying mechanisms of carbohydrate metabolisation. Restricting our focus on four commensal bacteria, includingB. xylanisolvens,B. thetaiotaomicron, S. variabileandR. intestinalis, carbon sources did significantly modulate the level of bacterial genes related to the enzymatic machinery involved in the metabolisation of dietary carbohydrates. This study provides a holistic view of the molecular strategies induced during the dynamic interplay between dietary carbohydrates with prebiotic potential and gut commensal bacteria.
Amygdalo-nigral inputs target dopaminergic and GABAergic neurons in the primate: a view from dendrites and soma
Authors: Fudge JL , Kelly EA , Love TM 
Published: January 2024

ABSTRACTThe central nucleus (CeN) of the amygdala is an important afferent to the DA system that mediates motivated learning. We previously found that CeN terminals in nonhuman primates primarily overlap the elongated lateral VTA (parabrachial pigmented nucleus, PBP, A10), and retrorubral field(A8) subregion. Here, we examined CeN afferent contacts on cell somata and proximal dendrites of DA and GABA neurons, and distal dendrites of each, using confocal and electron microscopy (EM) methods, respectively. At the soma/proximal dendrites, the proportion of TH+ and GAD1+ cells receiving at least one CeN afferent contact was surprisingly similar (TH = 0.55: GAD1=0.55 in PBP; TH = 0.56; GAD1 =0.51 in A8), with the vast majority of contacted TH+ and GAD1+ soma/proximal dendrites received 1-2 contacts. Similar numbers of tracer-labeled terminals also contacted TH-positive and GAD1-positive small dendrites and/or spines (39% of all contacted dendrites were either TH- or GAD1-labeled). Overall, axon terminals had more symmetric (putative inhibitory) axonal contacts with no difference in the relative distribution in the PBP versus A8, or onto TH+ versus GAD1+ dendrites/spines in either region. The striking uniformity in the amygdalonigral projection across the PBP-A8 terminal field suggests that neither neurotransmitter phenotype nor midbrain location dictates likelihood of a terminal contact. We discuss how this afferent uniformity can play out in recently discovered differences in DA:GABA cell densities between the PBP and A8, and affect specific outputs.Significance statementThe amygdala’s central nucleus (CeN) channels salient cues to influence both appetitive and aversive responses via DA outputs. In higher species, the broad CeN terminal field overlaps the parabrachial pigmented nucleus (‘lateral A10’) and the retrorubral field (A8). We quantified terminal contacts in each region on DA and GABAergic soma/proximal dendrites and small distal dendrites. There was striking uniformity in contacts on DA and GABAergic cells, regardless of soma and dendritic compartment, in both regions. Most contacts were symmetric (putative inhibitory) with little change in the ratio of inhibitory to excitatory contacts by region.We conclude that post-synaptic shifts in DA-GABA ratios are key to understanding how these relatively uniform inputs can produce diverse effects on outputs.
Gamma protocadherins in vascular endothelial cells inhibit Klf2/4 to promote atherosclerosis
Authors: Joshi Divyesh , Coon Brian G. , Chakraborty Raja , Deng Hanqiang , Fernandez-Tussy Pablo , Meredith Emily , Traylor James G. , Orr Anthony Wayne , Fernandez-Hernando Carlos , Schwartz Martin A. 
Published: January 2024

AbstractAtherosclerotic cardiovascular disease (ASCVD) is the leading cause of mortality worldwide1. Laminar shear stress (LSS) from blood flow in straight regions of arteries protects against ASCVD by upregulating the Klf2/4 anti-inflammatory program in endothelial cells (ECs)2–8. Conversely, disturbed shear stress (DSS) at curves or branches predisposes these regions to plaque formation9,10. We previously reported a whole genome CRISPR knockout screen11that identified novel inducers of Klf2/4. Here we report suppressors of Klf2/4 and characterize one candidate, protocadherin gamma A9 (Pcdhga9), a member of the clustered protocadherin gene family12. Pcdhg deletion increases Klf2/4 levels in vitro and in vivo and suppresses inflammatory activation of ECs. Pcdhg suppresses Klf2/4 by inhibiting the Notch pathway via physical interaction of cleaved Notch1 intracellular domain (NICD Val1744) with nuclear Pcdhg C-terminal constant domain (CCD). Pcdhg inhibition by EC knockout (KO) or blocking antibody protects from atherosclerosis. Pcdhg is elevated in the arteries of human atherosclerosis. This study identifies a novel fundamental mechanism of EC resilience and therapeutic target for treating inflammatory vascular disease.
Decipher the width of the
X(3872)
via the QCD sum rules
Authors: Wang Zhi-Gang 
Published: January 2024

In this work, we take the X(3872) as the hidden-charm tetraquark state with both isospin I=0 and I=1 components, then investigate the strong decays X(3872)→J/ψπ+π−, J/ψω, χc1π0, D*0D¯0, and D0D¯0π0 with the QCD sum rules. We take account of all the Feynman diagrams, and acquire four QCD sum rules based on rigorous quark-hadron duality. We obtain the total decay width about 1 MeV, which is in excellent agreement with the experiment data ΓX=1.19±0.21  MeV from the PDG, it is the first time to reproduce the tiny width of the X(3872) via the QCD sum rules, which supports assigning the X(3872) as the hidden-charm tetraquark state with the JPC=1++.

Published by the American Physical Society
2024
Royal Melbourne hospital family violence training framework 2018–2021
Authors: Fisher Caroline A. , Gill Helen , Galbraith Georgina , Sheridan Simone , Morris Emily , Bray Laura , Handley Emma , Withiel Toni D. 
Published: January 2024

Family violence is a significant social and public health problem. In 2015 a Royal Commission into Family Violence was established in Victoria, Australia, following a number of family violence deaths that received a high coverage in the media. The commission findings were released in 2016. These emphasised the significant physical and psychological harm that is caused by family violence, and that this has wide ranging community impacts. Among the Commission's 227 recommendations a number pertained specifically to improving the response of the healthcare system, with a whole-of-hospital model for responding to family violence recommend-ed for all public hospitals.
Royal Melbourne Hospital (RMH) received a state government grant as part of the SHRFV project. RMH was formally partnered with Tweddle Child and Family Health Service and Dental Health Services Victoria, and also worked with associated service NorthWestern Mental Health, as part of the project. This document outlines the RMH Family Violence Training Framework, a whole-of-hospital transformation change project designed to implement Recommendation 95 from the Royal Commission. All funded services were encouraged to adapt the SHRFV project model to suit the local environment of their health service. This document outlines the RMH approach. RMH specifically focused on using an evidence based research and evaluation framework with a focus on in-depth training, underpinned by a clinical champions network.
Coproduction, public sector innovation and governance: a systematic literature review
Authors: Baretta Julia Viezzer , Hoffmann Micheline Gaia , Militao Luciana , Farias Josivania Silva 
Published: January 2024

Purpose
The purpose of this study is examined whether coproduction appears spontaneously in the literature on public sector innovation and governance, the citizens’ role in coproduction and the implication of citizens’ participation in the governance of innovation networks.

Design/methodology/approach
The review complied with preferred reporting items for systematic reviews and meta-analyses (PRISMA) protocol. The search was performed in the Ebsco, Scopus and WOS databases. The authors analyzed 47 papers published from 2017 to 2022. Thematic and content analysis were adopted, supported by MAXQDA.

Findings
The papers recognize the importance of the citizens in public innovation. However, only 20% discuss coproduction, evidencing the predominance of governance concepts related to interorganizational collaborations – but not necessarily to citizen engagement. The authors also verified the existence of polysemy regarding the concept of governance associated with public innovation, predominating the term “collaborative governance.”

Research limitations/implications
The small emphasis on “co-production” may result from the search strategy, which deliberately did not include it as a descriptor, considering the research purpose. One can consider this choice a limitation.

Practical implications
Considering collaborative governance as a governing arrangement where public agencies directly engage nonstate stakeholders in a collective decision-making process that is formal, consensus-oriented and deliberative (Ansell and Gash, 2007), the forum where the citizen is supposed to be engaged should be initiated by public agencies or institutions and formally organized, as suggested by Österberg and Qvist (2020) and Campomori and Casula (2022). These notions can be useful for public managers concerning their role and how the forums structure should be to promote collaboration and the presence of innovation assets needed to make the process fruitful (Crosby et al., 2017).

Originality/value
Despite the collaborative nature of public innovation, the need for adequate governance characteristics, and the importance of citizens in the innovative process, most studies generically address collaborative relationships, focusing on interorganizational collaboration, with little focus on specific actors such as citizens in the governance of public innovation. Thus, it is assumed that the literature that discusses public innovation and governance includes the discussion of coproduction. The originality and contribution of this study is to verify this assumption.
Determinants of office rents: the role of physical and locational characteristics in Colombo
Authors: Marasinghe Raveena , Amarawickrama Susantha 
Published: January 2024

PurposeThis paper examines rent determinants and their relationship with commercial office property rents.Design/methodology/approachThe method adopted in this study differs from that of previous studies on this topic. Firstly, based on the survey of the viewpoints of experts, Relative Importance Index (RII) analysis was used to identify rent determinants and to rank and ensure their relevance and validity in the Sri Lankan context. Secondly, sampling of data related to 115 office properties collected from property tenants and landlords located within the central built-up area of Colombo City was conducted using a multi-methods approach to carry out an objective hedonic analysis of office rents.FindingsThis research utilizes RII and hedonic models to provide insights into determinants and relationships. Both analyses confirm that the three top drivers of commercial office rent are distance from the major town center, availability of parking space and the condition of the property. In addition to these three factors, hedonic models reveal that the age of the property and the availability of a conference hall also play a relevant role in explaining office rents. Given the disparities in the findings of the two methods, further examination was able to confirm that factors such as distance from the major town center, parking availability, age of the property, presence of a conference hall, building condition, floor size, business type and type of building are likely to influence commercial office rent. These findings reflect elements such as the quality, newness and better facilities of different office properties.Practical implicationsThis systematic study and analysis of office rent for the guidance of real estate investors can support sound investment decisions, potentially leading to more financially sound property development, reduced public debt levels and improved public-private financing. Further, the research findings offer valuable insights to real estate investors, developers and planners regarding location decisions for office development quality enhancements in future office developments.Originality/valueThis research provides fresh insights into the local scale office market, an area where limited evidence currently exists. Further, the methodology adopted provides evidence that hedonic analysis, supported by a multi-method approach, can mitigate the subjective judgments made by professionals.
The role of smart technologies in managing supply chain post pandemic: an exploratory scientific procedures and rationales for systematic literature review
Authors: Agarwal Pragati , Malhotra Sunita Kumari , Swami Sanjeev 
Published: January 2024

Purpose
The COVID-19 pandemic has brought unprecedented disruptions to global supply chains, compelling organizations to reevaluate their strategies for resilience and adaptability. In response, smart technologies (ST) have emerged as integral tools in post-pandemic supply chain management (SCM). This study aims to conduct an exploratory systematic literature review to comprehensively examine the evolving landscape of smart technology adoption in the context of SCM post-pandemic.

Design/methodology/approach
A systematic literature review has been conducted to examine the potential research contribution or directions in the field of ST and SCM. In total, 240 articles were shortlisted from the SCOPUS database in the chosen field of research. Bibliometric analysis was conducted by using VOSviewer to investigate the research trends in the area of SCM.

Findings
The review identifies key themes and trends, including supply chain resilience, digital transformation, enhanced visibility, predictive analytics and sustainability considerations. It explores the role of ST in fostering agility, transparency and risk mitigation within supply chains. Furthermore, eight clusters were identified to generate several thematic topics of ST in SCM. The results have evidenced a strong gap related to Industry 5.0 approaches for the supply chain field. A total of 240 publications, including journal articles, have been found in the literature. A total of 37 words, which were grouped in 8 clusters, have been identified in the data analysis.

Research limitations/implications
By synthesizing the current state of literature, this study provides valuable insights for practitioners, policymakers and researchers seeking to navigate the complexities of post-pandemic SCM in an increasingly digitized and interconnected world. The findings highlight the transformative potential of ST and offer a roadmap for further exploration in this critical domain.

Originality/value
In this paper, the development path of the field of ST in SCM during the pandemic and the research constructs are presented and potential research directions are based on the bibliometric method.
Green consumption by design: interaction experiences and customization intentions
Authors: Wang Chen , Zhang Yan , Zhang Ran 
Published: January 2024

PurposeThis study investigated the impacts of the interaction experiential customization (IEC) mode on consumers' information processing fluency and green customization intention (GCI) as well as the moderating effect of consumers' self-construal.Design/methodology/approachThis study conducted an online field experiment, questionnaire study and between-subjects laboratory experiment to test the hypotheses.FindingsIt was found that IEC had a significant positive effect on consumers' GCI. Moreover, consumer retrieval processing fluency played a partial mediating role in the relationship between IEC and GCI. In addition, consumers' self-construal moderated the “IEC? Three dimensions of processing fluency” relationships.Practical implicationsThe results emphasized the importance of IEC in influencing consumers' consumption intention in a green customization setting and have some practical implications, that is, companies have the opportunity to use appropriate digital choice architecture designs, which can enhance consumer processing fluency when promoting eco-friendly products in the customized consumption process, especially for independent consumers.Originality/valueThis study focused on the customization design on consumers' GCI and explained the mechanism of impact of IEC on improving consumers' processing fluency and GCI in a product customization setting based on the fluency theory. In addition, this study investigated the moderating effect of consumers' self-construal (independent vs interdependent) on their significant different information processing modes for low-carbon choices.