bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of positive and negative electrospray ionization, collision‐induced dissociation and tandem mass spectrometry to a study of the fragmentation of 6‐hydroxyluteolin 7‐O‐glucoside and 7‐O‐glucosyl‐(1 → 3)‐glucoside
Authors: Es‐Safi Nour‐Eddine , Kerhoas Lucien , Ducrot Paul‐Henri 
Published: August 2005

AbstractMass spectrometric methodology based on the combined use of positive and negative electrospray ionization, collision‐induced dissociation (CID) and tandem mass spectrometry (MS/MS) has been applied to the structural characterization of 6‐hydroxyluteolin 7‐O‐glucoside and 7‐O‐glucosyl‐(1 → 3)‐glucoside. In‐source fragmentation of both glycosides at an increased potential yielded the protonated and deprotonated aglycone, allowing CID spectra to be obtained. The differentiation between quercetin and 6‐hydroxyluteolin aglycones was achieved by product ion analysis of the protonated and deprotonated aglycone (m/z 303 and 301), that showed the characteristic product ions 1,3A at m/z 151 and 153 for quercetin, and m/z 167 and 169 for 6‐hydroxyluteolin, consistent with the trihydroxylated A‐ring skeleton. In the negative ion mode both glycosides were shown to undergo collision‐induced homolytic and heterolytic cleavages of the O‐glycosidic bond producing the aglycone radical‐anion [Y0–H]−. and Y product ions. At lower collision energy, various fragmentations involving the glucose moieties were observed with a relatively higher abundance for the monoglucoside compared to the diglucoside. In the latter case both the inner and the terminal glucose residues were involved in the fragmentations, giving useful information on the 1 → 3 interglycosidic linkage. CID MS/MS analysis of the sodiated molecules gave complementary information for the structural characterization of the studied compounds. Fragmentation mechanisms are proposed for the observed product ions. Copyright © 2005 John Wiley & Sons, Ltd.
Quantitation of DNA adduct of thymidylyl(3′‐5′)thymidine methyl phosphotriester by liquid chromatography/negative electrospray tandem mass spectrometry
Authors: Zhang Fagen , Bartels Michael J. , Pottenger Lynn H. , Gollapudi B. Bhaskar , Schisler Melissa R. 
Published: August 2005

AbstractA rapid and selective method based on liquid chromatography/electrospray tandem mass spectrometry (LC/ESI‐MS/MS) has been developed for the direct quantitation of a methyl phosphotriester DNA adduct, thymidyl (3′‐5′) thymidine [dTp(Me)dT] from enzymatic hydrolysates of DNA (either in vitro DNA or in cell culture) treated with MNU (N‐methyl‐N‐nitrosourea) or MMS (methyl methane sulfonate). The lower limit of quantitation was 2 ng/mL. Linearity of the calibration curve was greater than 0.999 from 2 to 1000 ng/mL. Intraday precision for four levels of quality controls ranged from 2.8 to 20.1%, and interday precision ranged from 2.9 to 5.6%. This method was used to quantify the levels of dTp(Me)dT in enzymatic hydrolysates of DNA obtained from a series of incubations of salmon testis DNA or mouse lymphoma cells with either MNU or MMS. Copyright © 2005 John Wiley & Sons, Ltd.
Individual and group meaning‐making in an urban third grade classroom: Red fog, cold cans, and seeping vapor
Authors: Southerland Sherry , Kittleson Julie , Settlage John , Lanier Kimberly 
Published: August 2005

AbstractWe examined third graders' understandings of condensation using an expanded notion of the Emergent Perspective, a reflexive consideration of individual and group meaning‐making situated in the culture of the classroom. Data were collected from two small groups of students in an inquiry‐based, urban classroom during a unit on the water cycle. Measures included conceptual pre‐/posttests, interviews, written work, and discourse analyses of a science lesson. Although we identified the supportive role of the teacher's explicit assessments of children's ideas, within the small groups, the force that most potently shaped meaning‐making was students' persuasive power, which was in part influenced by the rhetorical moves employed. Specifically, students' evaluative comments (a type of rhetorical move) about contributions of other group members seemed to be particularly persuasive in these groups. Evaluative comments, apart from students' academic status, were shown to be an important influence in not only social knowledge production but also in individual internalization. Our explanation focuses on the particular discursive practices as intellectual resources of urban students, but we are also mindful of the cognitive complexity of the material and the developmental abilities of the students. © 2005 Wiley Periodicals, Inc. J Res Sci Teach 42: 1032–1061, 2005
Transabdominal Transcolonic Polypectomy
Authors: Schmitt Edward Alexander 
Published: August 2005

AbstractUp until now, 2%–10% of colonic polyps larger than 2 cm have been considered colonoscopically unresectable. These were commonly treated by piecemeal resection and observation by colonoscopists and hemicolectomy by surgeons. Our minimally invasive “rural solution” in this situation is transcolonic resection through a mini‐laparotomy. We present 7 patients with large colonic villous adenomas not amenable to colonoscopic resection. Laporoscopy determined the location of the muscle‐splitting incision. The segment of bowel containing the polyp was exteriorized: antimesentric polyps were excised together with the corresponding colonic wall; polyps in mesenteric location were removed transmurally through an anterior colotomy. There was no surgical morbidity. Five of the 7 patients were discharged within 24 hours. Preoperative tattooing of the lesions and laparoscopic mobilization of the involved segment—when necessary—proved to be useful adjuncts. This seems to be an attractive option that may be superior to formal colectomy or repeated endoscopic piecemeal excision of large polyps. It provides colonoscopists with the option of avoiding having to resect very difficult polyps while not subjecting their patients to unnecessarily morbid operations.
Effect of Oral Glutamine Administration on Bacterial Tanslocation, Endotoxemia, Liver and Ileal Morphology, and Apoptosis in Rats with Obstructive Jaundice
Authors: Margaritis Vassilios G. , Filos Kriton S. , Michalaki Marina A. , Scopa Chrisoula D. , Spiliopoulou Iris , Nikolopoulou Vassiliki N. , Vagianos Constantine E. 
Published: August 2005

AbstractPostoperative complications in patients with obstructive jaundice remain increased when associated with endotoxemia and the inflammatory response due to gut barrier failure. Administration of glutamine has been proposed to maintain the integrity of the gut mucosa and thus reduce bacterial translocation (BT), but the effects of this pretreatment on apoptosis and histologic morphology of various organs affected by BT in obstructive jaundice have not been studied. We therefore studied the effects of oral glutamine supplementation on endotoxemia, BT, liver and terminal ileal morphology, and apoptosis in an experimental model of obstructive jaundice. A total of 60 male Wistar rats were randomly divided into four groups of 15 each: I, controls; II, sham‐operated; III, bile duct ligation (BDL); IV, BDL + glutamine (4.5 g/kg/day in drinking water). Ileal samples for histology, DNA and protein content, liver biopsies, mesenteric lymph nodes (MLNs) for culture, and portal and systemic blood samples for endotoxin measurements were obtained 10 days later. Compared to the controls, a significant increase in contaminated MLN and liver samples and increased endotoxemia were noted in group III (p < 0.01) but were significantly reduced in group IV (p < 0.05). Group IV also had a significantly higher number of mitoses per crypt (M/c) (p < 0.05), less apoptotic body counts (ABCs) (p < 0.05), and a higher DNA content than did group III (p < 0.05). Liver biopsies from group III displayed typical changes of large duct obstruction that significantly improved after glutamine treatment, with decreased ductular proliferation.We concluded that supplementation of oral glutamine in the presence of obstructive jaundice ameliorates BT, endotoxemia, and apoptosis and improves the ileal and liver histology.
Groin lymphadenectomy with Preservation of Femoral Fascia: Total Inguinofemoral Node Dissection for Treatment of Vulvar Carcinoma
Authors: Micheletti Leonardo , Bogliatto Fabrizio , Massobrio Marco 
Published: August 2005

AbstractThis article describes a new technique for groin lymphadenectomy with preservation of the femoral fascia based on correct embryologic and anatomic knowledge of inguinofemoral lymph node disposition and their exact relation with the fascial structures of Scarpa’s triangle. Scarpa’s triangle dissection follows a three‐step procedure: development of the side starting from the inguinal ligament; development of the angles proceeding from the apex where the saphenous vein is resected; and dissection of the fossa ovalis by grasping and elevating the entire block of adipose tissue containing the superficial inguinofemoral nodes and the stump of the great saphenous vein. This surgical step allows us to expose and remove en bloc the deep femoral nodes lying medial to the portion of the femoral vein located within the fossa ovalis. The total number of inguinofemoral nodes removed from a series of 156 patients operated on during 1981–2002 ranged from 8 to 35 (mean 20) bilaterally and from 4 to 18 (mean 10) unilaterally. The 5‐year survivals by stage were, respectively, 86.2% for stage Ib, 69.2% for stage II, 49.3% for stage III, and 13.3% for stage IVa; these figures are comparable to the survival rates reported by those performing the classic groin lymphadenectomy. Groin lymphadenectomy with preservation of the femoral fascia is thus an oncologically sound conservative procedure that can replace the classic Way’s technique, which involves femoral vessel skeletonization, and can be useful for treating malignant diseases requiring groin dissection.
Randomized Trial Comparing Quixil Surgical Sealant with Kaltostat Hemostatic Dressing to Control Suture Line Bleeding after Carotid Endarterectomy with ePTFE Patch Reconstruction
Authors: Sintler Martin P. , Mahmood Asif , Smith Simon R.G. , Simms Malcolm H. , Vohra Rajiv K. 
Published: August 2005

AbstractFollowing carotid endarterectomy (CEA), patch angioplasty provides a significant reduction in the risk of perioperative complications. The expanded polytetrafluoroethylene (ePTFE) patch is strong, is resistant to infection, and has low thrombogenicity; but it remains unpopular because of its tendency of prolonged bleeding at the suture line. We aimed to investigate whether the application of Quixil sealant to the suture line could improve the time to achieve hemostasis and reduce local blood loss when compared to a standard topical hemostat Kaltostat. A prospective, randomized trial of 20 patients undergoing CEA was undertaken. Patients were randomized to receive either Quixil sealant (treatment group) or topical Kaltostat (controls) as a hemostatic agent to the patch suture line. Hemostasis was defined as no bleeding at the suture line for 1 minute. Statistical analysis was performed using the Mann‐Whitney test. The two groups had a similar age and sex distribution. The mean age was 71 years, and there were seven men and three women in each group. The time to achieve hemostasis was significantly lower in the Quixil group (median 2.5 minutes, range 1–4 minutes) compared to the controls (median 17 minutes, range 7–59 minutes) (p < 0.001). Blood loss after clamp release was also significantly reduced in the Quixil group; median 24.5 ml (range 5.5–105.0 ml) versus 203 ml (range 54.5–817.0 ml) (p < 0.001). This study has demonstrated that Quixil human surgical sealant is an effective sealant of ePTFE patch suture holes and does not compromise the patch repair. It could be used during other vascular procedures involving ePTFE.
Surgical Treatment of Appendiceal Adenocarcinoid (Goblet Cell Carcinoid)
Authors: Bucher Pascal , Gervaz Pascal , Ris Frederic , Oulhaci Wassila , Egger Jean‐François , Morel Philippe 
Published: August 2005

AbstractAdenocarcinoid of the appendix is an infrequent tumor with histologic features of both adenocarcinoma and carcinoid tumor. Although its malignant potential remains unclear, adenocarcinoids seem to be biologically more aggressive than conventional carcinoids. The aim of this study was to analyze long‐term results of surgical treatment for appendiceal adenocarcinoid. A retrospective review (1991–2003) identified seven patients (median age 72, range 27–81 years) treated for appendiceal adenocarcinoid. The clinical data of these patients were reviewed. Follow‐up was complete for all patients (median 60 months, range 24–108 months). Most cases presented with associated acute appendicitis (71%). First intention surgery consisted of appendectomy (m = 6) and right hemicolectomy (m = 1). In three patients, additional surgical procedures were performed (right colectomy). Indications for colectomy were tumor size (three cases) associated with appendectomy margin invasion in one case. One patient with lymph node and peritoneal involvement experienced recurrence 9 months after hemicolectomy and died of the disease at 2 years. One patient subsequently died of colon carcinoma 6 years after adenocarcinoid treatment. Five patients were alive without disease at the time of the last follow‐up. Synchronous or metachronous colon carcinomas developed in three patients (43%). Our results suggest that appendectomy alone could be used for appendiceal adenocarcinoid provided that the tumor (1) is less than 1 cm; (2) does not extend beyond the appendix adventitia; (3) has less than 2 mitoses/10 high power fields; and (4) has surgical margins that are tumor free. Otherwise, carcinologic right colectomy seems to be indicated. The risk for developing colorectal adenocarcinoma seems to be extremely high in patients treated for appendiceal adenocarcinoid and warrants close follow‐up with colonoscopic screening.
Outcomes in Oncologic Surgery: Does Volume Make a Difference?
Authors: Bentrem David J. , Brennan Murray F. 
Published: August 2005

AbstractCommonly performed elective surgical procedures on the alimentary tract are carried out with low morbidity and low mortality in most hospitals in the United States. There are some procedures on the alimentary tract that are performed with a relatively low frequency and are associated with higher mortality. Volume is a surrogate marker associated with improved outcome, with relative differences being dependent on the complexity of the procedure and the frequency with which it is done. Both surgeon and institutional volume matters, but it seems that improved operative mortality can be reached with lower surgeon volume in high‐volume institutions. It appears that volume can be substituted in part for by specialization and training, with improved outcomes based on specialist credentials and fellowship training.
Behavioural factors related to metabolic control in patients with phenylketonuria
Authors: Crone M. R. , van Spronsen F. J. , Oudshoorn K. , Bekhof J. , van Rijn G. , Verkerk P. H. 
Published: September 2005

SummaryBackground. The objective of this study was to determine the importance of parental factors possibly related to dietary control in early and continuously treated patients with phenylketonuria (PKU). Methods. A questionnaire was disseminated among parents of 238 patients with PKU born after the nationwide introduction of newborn screening for PKU (1 September 1974) until 31 December 1995. The questionnaire was based on a behavioural model measuring people's attitudes, subjective norms, and self‐efficacy. Dietary control was defined on the basis of mean phenyl‐ alanine (Phe) concentration of the PKU patients measured between 1 January 1994 and 31 December 1996. Results. Response rate was 71%. Attitudes: children of parents who believed that their child adheres well to the diet, even if his or her Phe concentrations are sometimes too high, had lower Phe concentrations than children of parents who disagree with this statement (adjusted difference −103 μmol/L, p < 0.001). Subjective norm: Phe concentrations were higher when parents answered that their relatives did not approve when their child deviates from the diet (p = 0.004). Self‐efficacy: children of parents who reported difficulties in having their child eat the synthetic protein substitute three times a day had higher Phe concentrations than those of parents who did not have such difficulties (adjusted difference 156 μmol/L, p = 0.007). Conclusion. More attention should be given to parents having their child eat the synthetic protein substitute at least three times a day and to teaching parents to keep strictly to the diet without being too rigid. These factors were strongly associated to dietary control and may be amenable to change.
Body composition in young adults with inborn errors of protein metabolism—A pilot study
Authors: Wilcox G. , Strauss B. J. G. , Francis D. E. M. , Upton H. , Boneh A. 
Published: September 2005

SummaryThe natural history of inborn errors of protein metabolism and the long‐term effects of prescribed semisynthetic therapeutic diets are largely unknown. We assessed body composition, measuring body‐fat mass and distribution, fat‐free mass, total body protein, total body potassium, bone density and skeletal muscle mass, in young adults (age > 18 years; 6 female, 5 male) with inborn errors of protein metabolism maintained on long‐term low‐protein diets, compared with controls. Female patients were significantly shorter (159.4 cm vs 169.2 cm, p = 0.013) and had higher BMI (25.3 vs 22.0 kg/m2, p < 0.05), abdominal to gluteal circumference ratio (0.84 vs 0.73, p = 0.011), percentage body fat (42.3% vs 29.5%, p < 0.005) and ratio of central to peripheral body fat (1.15 vs 0.86, p < 0.05) than controls. Male patients had lower height‐adjusted total body bone mineral content (0.9 vs 1.02 g/m2, p < 0.04) and skeletal muscle mass (31.1 vs 36.3 kg, p < 0.04) than controls. Compared with controls, patients'nitrogen index was significantly lower (0.91 vs 1.03, p < 0.01), consistent with lower total body protein. Potassium index was significantly higher (121.2% vs 110.4%, p < 0.03), consistent with higher body cell mass, or intracellular water. Documentation of body composition in larger patient series is important to elucidate whether these results reflect increased risks (hence opportunities for prevention) of bone disease, metabolic syndrome and cardiovascular disease in this population.
A new protein substitute for adolescents and adults with maple syrup urine disease (MSUD)
Authors: Hallam P. , Lilburn M. , Lee P. J. 
Published: September 2005

SummaryAim.The aim was to study the efficacy and acceptability of MSUD Express in adolescent and adult patients with maple syrup urine disease (MSUD). Background. There are difficulties associated with current protein substitutes designed for adolescents and adults with MSUD. This can affect their metabolic control. MSUD Express is a new low‐volume, nutritionally complete protein substitute, free from leucine, isoleucine and valine and designed specifically for older patients with MSUD. Methods. Four patients with MSUD were included in the trial (aged 16–41 years). Product dosage was 4× 25 g sachets per day, providing 1264 kJ and 72 g amino acids: more than adequately meeting requirements for all micronutrients. Results. At the start of the study, the mean (range) of the previous eight leucine concentrations (on MSUD Aid III) was 564 μmol/L (430–817 μmol/L) and during the study (on MSUD Express) was 382 μmol/L (181–603 μmol/L). The average percentage fall in mean leucine concentrations was 32%. In patients 2, 3 and 4, low leucine levels resulted in an increase in the natural protein allowance per day. MSUD Express was rated as ‘excellent’ or ‘good’ by all patients on the basis of appearance, taste, smell and texture. All patients found the product ‘very easy’ to prepare and found it ‘easy’ or ‘very easy’ to take outside the home. Conclusion. All patients thought the preparation of MSUD Express was ‘very easy’. Metabolic control (mean leucine concentrations) improved in all of the patients. In three patients this meant that an increase in the amount of natural protein in the diet was possible. All patients elected to stay on MSUD Express after the trial period. MSUD Express appears to be an effective and acceptable product, offering an alternative to current protein substitutes available for older people with MSUD.
Heparan sulfate levels in mucopolysaccharidoses and mucolipidoses
Authors: Tomatsu S. , Gutierrez M. A. , Ishimaru T. , Peña O. M. , Montaño A. M. , Maeda H. , Velez‐Castrillon S. , Nishioka T. , Fachel A. A. , Cooper A. , Thornley M. , Wraith E. , Barrera L. A. , Laybauer L. S. , Giugliani R. , Schwartz I. V. , Frenking G. Schulze , Beck M. , Kircher S. G. , Paschke E. , Yamaguchi S. , Ullrich K. , Isogai K. , Suzuki Y. , Orii T. , Noguchi A. 
Published: September 2005

SummaryGlycosaminoglycans are accumulated in both mucopolysaccharidoses (MPS) and mucolipidoses (ML). MPS I, II, III and VII and ML II and ML III patients cannot properly degrade heparan sulphate (HS). In spite of the importance of HS storage in the metabolic pathway in these diseases, blood and urine HS levels have not been determined systematically using a simple and economical method. Using a new ELISA method using anti‐HS antibodies, HS concentrations in blood and urine were determined in MPS and ML II and ML III patients. HS concentrations were determined in 156 plasma samples from MPS I (n = 23), MPS II (n = 26), MPS III (n = 24), MPS IV (n = 62), MPS VI (n = 5), MPS VII (n = 5), ML II (n = 8) and ML III (n = 3), and 205 urine samples from MPS I (n = 33), MPS II (n = 33), MPS III (n = 30), MPS IV (n = 82), MPS VI (n = 7), MPS VII (n = 9), ML II (n = 8) and ML III (n = 3). The ELISA method used monoclonal antibodies against HS. MPS I, II, III and VII and ML II and III patients had significant elevation in plasma HS, compared to the age‐matched controls (p < 0.0001). Eighty‐three out of 89 (93.3%) of individual values in the above MPS types and ML were above the mean +2SD of the controls. In urine samples, 75% of individual values in patients with those types were above the mean +2SD of the controls. In contrast to the previous understanding of the HS metabolic pathway, plasma HS levels in all five MPS VI and 15% of MPS IV patients were elevated above the mean +2SD of the controls. These findings suggest that HS concentration determined by ELISA, especially in plasma, could be a helpful marker for detection of the most severe MPS I, II, III, VI and VII and ML II, distinguishing them from normal populations.
Living with phenylketonuria: Perspectives of patients and their families
Authors: Bilginsoy C. , Waitzman N. , Leonard C. O. , Ernst S. L. 
Published: September 2005

SummaryThis study surveyed PKU patients and their primary caretakers to assess their current management practices, the barriers to effective management, and the potential utility of a home monitor in managing PKU. A survey instrument was mailed to caretakers of all 50 patients with PKU in Utah between the ages of 2 and 18 years in 1997 (response rate 64%). It included separate components for caretakers and patients aged 10 to 18 years. Although there was uneven compliance with recommended practices, caretakers universally recognized the negative consequences of not adhering to the low‐protein diet. There was, however, disagreement regarding such consequences among the older children surveyed. The primary obstacles cited to better adherence were time constraints and stress associated with food preparation and record‐keeping, and the restrictions imposed on social life. Phenylalanine test results were regarded as the principal signal for the need for dietary adjustment. Despite the facts that obstacles to dietary adherence are multifaceted and that no single intervention would therefore serve as a panacea, a large majority of respondents believed a home monitor would facilitate better management of PKU through more regular and timely feedback.