bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Frozen Elephant Trunk Procedure Using New Russian Hybrid Prosthesis “Soft Elephant Trunk”: the Early Experience
Authors: Charchyan E. R. , Breshenkov D. G. , Belov Yu. V. 
Published: October 2023

Aim To present the first experience of performing the Frozen Elephant Trunk (FET) surgery in patients with thoracic aorta disease using a new Russian hybrid stent graft “Soft Elephant Trunk”.Material and methods Between 2014 and 2021, 170 patients with thoracic aortic disease underwent complete aortic arch replacement using the FET technique. In 70 of these cases (since June 2019), a hybrid graft “Soft Elephant Trunk” was used. A specific feature of this graft is the conical reduction of the radial force of nitinol crowns and the soft distal end without radial force. The study endpoints were early postoperative results, as well as the absence of reoperations on the aorta, absence of stent-graft-associated complications, including distal stent-graft-induced fenestration, and survival at a mid-term follow-up of up to 2.5 years.Results Interventions were performed in patients with A type aortic dissection (n=51; 72.9 %), type B aortic dissection (n=13; 18.6 %), and thoracoabdominal aortic aneurysm (TAAA) (n=6; 8.5 %). In 14 (20 %) of these cases, interventions were performed after a primary intervention on the proximal aorta. Acute aortic dissection was diagnosed in 17 (24.3 %) cases. Aortic root replacement was performed in 21 (30 %) cases, David procedure in 5 (7.2 %) cases, Bentall-DeBono procedure in 11 (15.7 %) cases, and supracoronary graft placement in 33 (47.1 %) cases. There were no cases of paraplegia or paraparesis. Respiratory insufficiency was observed in 8 (11.4%) cases. In one (1.4%) case, acute renal failure developed, which required renal replacement therapy. In-hospital mortality was 4.3 % (3 patients died). Mean follow-up duration was 9 [4.25; 16] months. Three-year survival was 94 % (95 % confidence interval, CI: 88–99.9) and absence of reoperation was 96.6 % (95 % CI: 90.1–100). There were no cases of distal stent-graft-induced fenestration in this group.Conclusion The new hybrid graft, due to its specific structure, provides prevention of distal stent-graft-induced fenestration and, thereby, a stable long-term result. Using this stent-graft is effective in patients with thoracic aorta pathology undergoing the FET surgery. However, further studies are needed to determine potential advantages and disadvantages of this new hybrid graft, to obtain long-term results and accumulate experience.
The Protective Effect of Polymorphisms rs2681472 and rs17249754 of the ATP2B1 Gene Against Coronary Artery Disease and Hypertension is Abolished by Tobacco Smoking
Authors: Kononov S. I. , Azarova Yu. E. , Klyosova E. Yu. , Bykanova M. A. , Solodilova M. A. , Polonikov A. V. 
Published: October 2023

Aim To study the relationship of single nucleotide polymorphisms rs2681472 and rs17249754 in the ATP2B1 gene with risk of ischemic heart disease (IHD) and arterial hypertension (AH) among residents of Central Russia and to evaluate the trigger role of smoking as a risk factor for development of IHD and AH in carriers of ATP2B1 gene polymorphic variants.Material and methods The study included DNA samples from 1960 residents of Central Russia of Slavic origin. Among them, there were 1261 patients with cardiovascular diseases and 699 healthy persons. The vast majority of patients had both IHD and AH. Genotyping was performed using the iPLEX technique on a MassARRAY-4 genomic mass-spectrometer. The relationship of ATP2B1 alleles, genotypes, and haplotypes with the risk of diseases was calculated by logistic regression analysis with adjustments for sex and age.Results Carriage of AG and GG (rs2681472) genotypes and GA (rs17249754) genotype was associated with a reduced risk of both IHD (p=0.0057 and p=0.022 for rs2681472 and rs17249754, respectively) and AH (p=0.016 and p=0.036, respectively). Rare rs2681472G-rs17249754G and rs2681472A-rs17249754A haplotypes were associated with a reduced risk of IHD (odds ratio, OR, 0.22; 95 % CI: 0.11–0.46, p=0.0001) and AH (OR, 0.22; 95 % CI: 0.10–0.47, p=0.0001). Analysis of the groups stratified by the smoking status showed that in smokers, the studied polymorphic variants did not have a protective action with respect of either IHD or AH. However, in non-smokers, the genotypes AG and GG rs2681472 (OR, 0.62; 95 % CI: 0.47–0.80, p=0.0004) and GA rs17249754 (OR, 0.61; 95 % CI: 0.47–0.81, p=0.0004) were associated with a reduced risk of IHD and AH (OR, 0.63; 95 % CI: 0.48–0.83, p=0.0004 for rs2681472; OR, 0.63; 95 % CI: 0.48–0.83, p=0.001 for rs17249754), as well as the carriage of the minor alleles rs2681472‑G and rs17249754‑A.Conclusion It was shown for the first time that the polymorphic variants rs17249754 and rs2681472 of the ATP2B1 gene are associated with a reduced risk for IHD and AH only in non-smokers.
Variability of Measurement and Feasibility of Assessing Changes in Brachiocepahlic Atherosclerotic Plaque After Acute Coronary Syndrome
Authors: Bershtein L. L. , Boldueva S. A. , Kochanov I. N. , Lunina M. D. , Naiden T. V. , Evdokimov D. S. , Tandelov B. M. , Podmetin P. S. , Zbyshevskaya E. V. , Gumerova V. E. , Savinova E. B. , Piltakyan V. H. , Sayganov S. A. 
Published: October 2023

Aim Analysis of inter- and intra-study variability of changes in the atherosclerotic plaque (ASP) total height and total area, the main quantitative indexes that were planned to be used in the present study for assessment of the atherosclerotic load of carotid arteries.Material and methods The incidence of recurrent cardiovascular complications (CVC) within 1 year after acute coronary syndrome (ACS) ranges from 7-9 % (in studies) to 34 % (in clinical practice). This indicates insufficient efficacy of traditional approaches to secondary prevention of coronary heart disease. We proposed a study to test a hypothesis that the dynamics of ASP parameters in carotid and subclavian regions can serve as an alternative criterion for the adequacy of secondary prevention after ACS. The analysis was performed on subgroups of main study participants. These patients had ACS of any type documented by coronary angiography with an ASP confirmed by ultrasound of the brachiocephalic arteries (BCA) during the index hospitalization. BCA ultrasound was performed to analyze the inter- and intra-study variability of BCA atherosclerotic load, the ASP total height (Hsum) and total area (ASPTA), in 20 and 24 patients of the main study, respectively. Results of the repeated ultrasound were evaluated in 30 patients of the main study after 6 months of follow-up.ResultsThe inter-study variability of each index was significantly higher than the intra-study variability which was consistent with results of previous studies. The intra-study variability of Hsum was 0.10 (95 % confidence interval, CI – 0.23–0.44) mm and ASPTA, 1.05 (95 % CI, – 0.54–2.63) mm2. The variability values were considerably smaller than the changes for 6 months: Hsum, 0.92 (95 % CI, – 0.64–2.49) mm and ASPTA, 3.67 (95 % CI, 0.42–6.91) mm2, although the difference did not reach statistical significance. The above results were obtained at an early stage of the study during the adaptation of specialists to the protocol.Conclusion The study results suggest a possibility of a fairly reliable assessment of the dynamics of quantitative indexes of carotid ultrasound 6 months after ACS.
Comparative evaluation of the effectiveness of various solvents on dissolving efficacy of gutta percha- An in vitro- study
Authors: Bukhari Syed Manzoor Ul Haq , Rashid Sheeeban , Bhat Rahil 
Published: October 2023

This study's objective was to compare how well xylene, chloroform, turpentine oil, halothane, and orange oil dissolved materials at three different immersion time intervals.For the investigation. An ISO no. 40 cone and gutta-percha cones with a 0.06 taper were gathered. In each of the tested solvents, five batches of samples were made and submerged for 6, 12, and 18 minutes. To ascertain the process of gutta-percha dissolution in the solvents, the weight before immersion and the weight after immersion in the solvents were measured on a digital analytical scale. Analysis of Variance (ANOVA) and multiple comparisons with Scheffe's test (p 0.05) were used to statistically analyse the data. With xylene, the best solvency capacity was attained. Halothane, orange oil, and chloroform all had comparable dissolving abilities.
Penerapan simple multi attribute rating technique exploiting ranks method dalam pemilihan jenis tanaman pangan di Desa Bandar Kwala
Authors: Dewi Sartika , Cipta Hendra , Dur Sajaratud 
Published: October 2023

In the field of agriculture in Indonesia, there are forms or types of plants, one of which is food crops. Currently, the food crops that develop in Bandar Kwala village are rice, corn, peanuts, and cassava. So that there is competition for nutrient absorption between plants and many types of plant-disturbing organisms that appear and cause farmers to experience crop failure, the selling price in the market is not proportional to the price of the seeds they buy and the care they do. For each type of plant that is planted, it is necessary to pay attention to the factors that affect the growth of a plant, one of which is land management, seeding, fertilization and pest control. The purpose of this research is to apply the SMARTER method to get the ranking of plants suitable for planting on agricultural land in Bandar Kwala village based on food crop criteria. In the SMARTER method, the ranking results are obtained by looking at the final value obtained by each alternative, where the alternative rice plant gets a final value of 0.9581 as the first rank, the cassava plants alternative gets a final value of 0.2628 as the second rank, the alternative to peanut plant gets the final score was 0.2354 as the third rank, and the alternative maize crop obtained a final score of 0.0899 as the fourth rank.Keywords: Plant, decision, rank, SMARTER MSC2020: 90B50
Investigation of the Lipopolysaccharide Cluster Structure in the Genomes of <i>Vibrio cholerae</i> Rough Variants
Authors: Podoinitsyna O. A. , Kruglikov V. D. , Levchenko D. A. , Vodop’yanov A. S. , Ezhova M. I. , Noskov A. K. 
Published: October 2023

Determination of Vibrio cholerae affiliation to one or another serogroup may meet some difficulties in cases of atypical agglutination with diagnostic cholera sera. The study of genetic determinants that allows for identifying a serogroup is a relevant task in monitoring of surface water body contamination with cholera vibrios.The aim of the work was to compare the structural organization (quantitative and qualitative gene composition) of LPS clusters in V. cholerae rough variants.Materials and methods. We used Illumina MiSeq for the whole genome sequencing; SPAdes software (version 3.11.1) for de novo assembly; and blastn (v. 2.5.0) for gene searching. GeneMarkS software was deployed for annotation of the genes incorporated in the clusters; nucmer – for searching homologous sites. Visualization of O-LPS clusters was carried out by means of SnapGene Viewer.Results and discussion. Strains of V. cholerae rough variants had diverse gene clusters responsible for O-antigen biosynthesis. We have identified three types of O-LPS clusters with different size and number of genes. Unique DNA sites, common to the whole group of V. cholerae rough variants, have not been detected. Two genes present in all rough strains have been defined, but they are not unique for this group of strains and can be found in representatives of other serogroups. For two types of clusters, a region containing the IS‑element, common with V. cholerae O1, has been revealed.
Detection of the Genetic Material of the Viruses Tacheng uukuvirus and Sara tick phlebovirus in Taiga Ticks Collected in the Sverdlovsk, Tomsk Regions and Primorsky Territory of Russia and Their Phylogeny
Authors: Tupota N. L. , Ternovoi V. A. , Ponomareva E. P. , Bayandin R. B. , Shvalov A. N. , Malyshev B. S. , Tregubchak T. V. , Bauer T. V. , Protopopova E. V. , Petrova N. K. , Zhebrovskaya E. V. , Burukhina E. G. , Khomichuk T. F. , Agafonov A. P. , Maksyutov R. A. , Loktev V. B. 
Published: October 2023

Extensive spread of tick-borne diseases poses a significant problem for public health and the health of the population living in endemic areas.The aim of the study was to search, analyze genetic material and identify new viral agents of the Phenuiviridae family in taiga ticks collected in Asian regions of Russia using the method of high throughput sequencing.Materials and methods. The study involved 1460 taiga ticks collected in suburban areas of the Tomsk, Yekaterinburg and Primorsky Territory. The genetic material isolated from ticks was sequenced using Illumina technology followed by phylogenetic analysis.Results and discussion. Analysis of the sequencing results made it possible to detect extended nucleotide sequences of the L-gene fragment characteristic of the Phenuiviridae family viruses. We were able to identify 20 nucleotide sequences the length of 250 bp on average in homogenates of Ixodes persulcatus ticks. Eighteen isolates have been identified as members of the genus Uukuvirus and two isolates have been assigned to the genus Phlebovirus, Phenuiviridae family. Phylogenetic analysis has shown that all isolates of the genus Uukuvirus fall under the cluster of Tacheng tick virus 2 belonging to the species Tacheng uukuvirus. They form a separate phylogenetic group which is closely related to two Romanian variants of 2019. Tacheng tick virus 2 was detected in all three surveyed regions of the Asian part of Russia. Two Tomsk isolates of phlebovirus were classified as Sara tick phlebovirus and they clustered with two isolates of phleboviruses from Karelia. Thus, the genetic material of Tacheng tick virus 2 and Sara tick phlebovirus belonging to two genera of the family Phenuiviridae was found in I. persulcatus ticks collected in three geographically different regions of the Asian part of Russia.
Evaluation of the Analytical Capabilities of MALDI-TOF Mass Spectrometry in the Molecular Typing of <i>Bacillus anthracis</i>
Authors: Ul’shina D. V. , Kovalev D. A. , Eremenko E. I. , Semenova O. V. , Ryazanova A. G. , Bobrysheva O. V. , Zhirov A. M. , Kulichenko A. N. 
Published: October 2023

The aim of the work was to compare the discriminating capacity of the canSNP13 genotyping and MALDI‑TOF mass spectrometry methods based on the results of the study of anthrax pathogen strains belonging to the two main genetic lines A and B.Materials and methods. 73 Bacillus anthracis strains from the collection of microorganisms of the Stavropol Research Anti-Plague Institute of the Rospotrebnadzor were investigated. Protein profiling was carried out using a Microflex mass spectrometer, data processing – in the environment of the statistical programming language “R”.Results and discussion. It has been experimentally confirmed that the proposed approach for differentiating proteotypes of B. anthracis strains with a discrimination index of 0.952 exceeds the one for the canSNP typing method and is comparable to the discrimination index for the MLVA31 method. The correlation of the results of strain clustering during typing by MALDI-TOF mass spectrometry and canSNP genotyping reaches 95 % as regards the division into the main genetic lines A and B. The studied strains of anthrax microbe, which mostly fall under phylogenetic groups of lineage A, represent more than a dozen protein profiles, which may be due to differences in the level of protein expression in strains of each canSNP genotype. MALDI-TOF mass spectrometry makes it possible to obtain the results comparable with genetic tests, has a better discriminating capacity compared to canSNP typing, and is easier to perform.
Assessment of Stability of Chemical Cholera Vaccine in a New Primary Packaging
Authors: Gromova O. V. , Vorob’eva S. A. , Volokh O. A. , Klokova O. D. , Livanova L. F. , Belyakova N. I. , Popova E. Z. , Lobovikova O. A. , Shul’gina I. V. , Shvidenko I. G. , Nikiforov A. K. 
Published: October 2023

The bivalent chemical cholera vaccine is the only drug for the prevention of cholera registered in the Russian Federation. The vaccine has been produced in glass bottles containing 210 tablets. At the same time, modern trends dictate the need to produce the drug in varying dispensing and more practical packaging for the convenience of the consumer.The aim of the work was to study the stability of the properties of the immunobiological medicinal product “Bivalent chemical cholera vaccine” with modified filling and in new primary packaging.Materials and methods. When studying the quality of bivalent chemical cholera vaccine batches, physicochemical parameters, formaldehyde content, specific activity and safety, abnormal toxicity, immunogenicity, and microbiological purity were assessed. Stability in terms of “specific activity” was evaluated using dot immunoassay.Results and discussion. As a result of this work, the use of several dispensing options and new primary packaging of cholera vaccine has been experimentally substantiated. The stability of the finished vaccine preparation has been established in the “accelerated aging” test and during long-term storage. The possibility of using dot immunoassay with a conjugate based on staphylococcal protein A, labeled with colloidal gold, to monitor the stability of cholera vaccine has been experimentally demonstrated.
Comparative Molecular-Genetic Analysis of <i>Francisella tularensis</i> Strains Isolated in the Rostov Region in 2020 and Genome Sequences of the Strains Collected in Various Regions of the World
Authors: Vodop’yanov A. S. , Pisanov R. V. , Vodop’yanov S. O. , Tsimbalistova M. V. , Pichurina N. L. , Sorokin V. M. , Pavlovich N. V. , Noskov A. K. 
Published: October 2023

Six cultures of tularemia microbe from fallen and captured live animals were isolated during epizootiological monitoring in the steppe focus in the south-east of the Rostov Region in 2020 against the background of extensive epizootics in the populations of the common vole Microtus arvalis obscurus and the public vole Microtus socialis.The aim of the work was to develop an SNP-typing scheme and to conduct a comparative study of the phylogenetic relations between Francisella tularensis strains isolated in the Rostov Region (2020) and strains from other regions.Materials and methods. Genome-wide sequencing was performed on the MiSeq Illumina platform. The author’s software GeneExpert, PrimerM and VirtualPCR, written in the Java programming language, were used for the analysis.Results and discussion. The strains of tularemia agent, isolated on the territory of the Rostov Region in 2020, can be allocated to two different clusters. It is established that two strains of tularemia pathogen (F0884 and F0889) isolated in Turkey are genetically close to some isolates circulating in the Rostov Region. A unique INDEL marker characteristic of this group of strains has been identified. The comparison of our proposed typing scheme with the scheme of “canonical” SNPs has showed a fairly good consistency and convergence of results within large clusters, meanwhile using a set of 6626 SNPs allows for differentiating the strains within one canSNP type. It is revealed that the vaccine strain has a common canSNP type with clinical and natural strains. A set of SNP markers has been selected for comparative analysis. A new INDEL marker that enables intraspecific typing of F. tularensis has been discovered and the possibility of its application in vitro and in silico has been comfirmed.
COVID‑19 Pandemic: Effect of Specific and Non-Specific Prevention Measures on the Risk of SARS‑CoV‑2 Infection in Employees of Healthcare Organizations
Authors: Egorov I. A. , Smirnova S. S. , Mishchenko V. A. , Vyalykh I. V. , Markaryan A. Yu. , Zhuikov N. N. , Aver’yanov O. Yu. , Smirnova V. A. , Bol’shakova A. N. , Alferov S. Yu. , Babanova A. V. , Kilyachina A. S. , Pushkareva N. A. , Semenov A. V. 
Published: October 2023

From the very onset of SARS‑CoV‑2 spreading, active involvement in the COVID‑19 epidemic process made the healthcare professionals (HCPs) a vulnerable group with higher risks of contracting the disease, severe course and fatal outcome.The aim. We aimed at studying the impact of specific and non-specific preventive measures on the risk of SARS‑CoV‑2 infection among the HCPs in a large industrial region settings during the COVID‑19 pandemic.Materials and methods. We analyzed the data obtained using 1 905 questionnaires for the personnel of infectious hospitals for treatment of COVID‑19 patients and non-core healthcare facilities in a large industrial region during the COVID‑19 pandemic, 100 questionnaires on adherence of the employees to hand hygiene and antiseptics, the results of the tests for specific IgG to the SARS‑CoV‑2 nucleocapsid (2 200 samples), as well as the results of assessment of viral and bacterial contamination of the outer surface of the personal protective equipment for infectious hospital staff (108 specimens).Results and discussion. In the course of the study, an effect of failure in the implementation of some specific and non-specific preventive measures on an increase in the incidence of COVID‑19 in various healthcare professionals has been identified. The COVID‑19 pandemic once again emphasized the need to save lives and health of the HCPs as socially significant category of citizens under the spread of pathogens with high epidemic potential, applying a complex of specific and non-specific measures that are not mutually exclusive.
Assessment of the Epidemiological Effectiveness of the Temporary Transfer of Schoolchildren to Distance Learning during the Spread of COVID‑19 by the Example of the Saratov Region
Authors: Zimirova A. A. , Kuklev V. E. , Safronov V. A. , Kuklev E. V. , Vyatkin I. N. 
Published: October 2023

Temporary closure of schools is an effective anti-epidemic measure during periods of rising incidence of acute respiratory viral infections and influenza, which is confirmed by the results of a large number of scientific works. In particular, the effectiveness of this measure consists in reducing the incidence among students. In the context of the spread of COVID‑19 in the Russian Federation, like in many other countries, the temporary transfer of schoolchildren to distance learning has also been repeatedly introduced; however, it remains unclear whether the measure under consideration is effective in relation to the new coronavirus infection.The aim of the study was to assess the epidemiological effectiveness of the temporary transfer of students to distance learning during the spread of COVID‑19 in the Saratov Region.Materials and methods. The analysis was carried out on the basis of statistical data of the Rospotrebnadzor Administration in the Saratov Region on the incidence of COVID‑19 for the period of 2020–2022, as well as materials from published scientific papers.Results and discussion. Analysis of the data on the school closure in 2020 has revealed a decrease in the incidence among schoolchildren (–42.9 %) and an increase in the incidence among people aged 65 years and older (+7.2 %); in 2021, there was a decrease in the incidence among schoolchildren (–15.6 %) and an increase in the indicator value as regards the elderly (+17.8 %); in 2022, another school closure led to an increase in the incidence of COVID‑19 among schoolchildren (+7.8 %) and people over 65 years old (+44.2 %). Based on the results obtained, one can conclude that the measure under consideration is inexpedient due to the fact that the most vulnerable category of the population (persons aged 65 years and older) is actively involved in the epidemic process, which in turn leads to a significant deterioration of the epidemic situation.