bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the efficacy of Simparica Trio® in the prevention of the transmission of Babesia canis by infected Dermacentor reticulatus to dogs
Authors: Borowski Stasia , Viljoen Alta , D’Hanis Lina , Mahabir Sean , Geurden Thomas 
Published: February 2024

Abstract
Background
Babesia canis is a clinically relevant vector-borne pathogen in dogs, and its presence is expanding. The efficacy of Simparica Trio® (Zoetis) in the prevention of B. canis transmission was evaluated at the minimum recommended label dose of 1.2 mg/kg sarolaner, 24 µg/kg moxidectin and 5 mg/kg pyrantel per kg bodyweight.

Methods
Twenty-four (24) dogs were randomly allocated to either a placebo-treated group or one of two treatment groups with Simparica Trio. Dogs were infested with B. canis-infected Dermacentor reticulatus ticks 21 or 28 days after treatment administration. Blood samples for antibody and DNA detection were collected from each dog prior to tick infestation until 28 days after infestation. A dog was defined as being B. canis positive if it tested positive by both an indirect immunofluorescence assay (IFA) and PCR at any time during the study.

Results
No treatment-related adverse reactions were recorded during the study. All placebo-treated animals displayed clinical signs due to babesiosis and tested positive on both IFA and PCR. None of the Simparica Trio-treated animals displayed any clinical symptoms or tested positive, resulting in a 100% efficacy in the prevention of canine babesiosis (P < 0.0001).

Conclusions
A single treatment with Simparica Trio at the minimum recommended label dose of 1.2 mg/kg sarolaner, 24 µg/kg moxidectin and 5 mg/kg pyrantel per kg bodyweight prevents the transmission of B. canis by infected D. reticulatus to dogs for at least 28 days.

Graphical Abstract
Epigenetics of prenatal stress in humans: the current research landscape
Authors: Dieckmann Linda , Czamara Darina 
Published: February 2024

AbstractFetal exposure to prenatal stress can have significant consequences on short- and long-term health. Epigenetic mechanisms, especially DNA methylation (DNAm), are a possible process how these adverse environmental events could be biologically embedded. We evaluated candidate gene as well as epigenome-wide association studies associating prenatal stress and DNAm changes in peripheral tissues; however, most of these findings lack robust replication. Prenatal stress-associated epigenetic changes have also been linked to child health including internalizing problems, neurobehavioral outcomes and stress reactivity. Future studies should focus on refined measurement and definition of prenatal stress and its timing, ideally also incorporating genomic as well as longitudinal information. This will provide further opportunities to enhance our understanding of the biological embedding of prenatal stress exposure.
Effects of dialogic reading for comprehension (LuDiCa) on the social interaction of autistic adolescents and their peers
Authors: Guevara Victor Loyola de Souza , Coêlho Raquel Freire , Flores Eileen Pfeiffer 
Published: February 2024

Abstract
Background
In today’s contemporary world, relationships take a prominent role in the lives of adolescents. However, challenges related to mutual understanding and a lack of inclusive environments can often lead to autistic teens feeling excluded.

Objective
In order to assess the impact of naturalistic interventions on interpersonal relationships, we conducted an experimental test utilizing Dialogic Reading for Comprehension (LuDiCa) in online reading circles with groups comprising both autistic and neurotypical adolescents. Our focus was on exploring its relevance for enhancing social interaction, particularly in terms of conversational acts, sharing experiences, initiations, and questions.

Methods
Five autistic and five neurotypical students between 11 and 15 years old from a public school in Brasilia, Federal District, participated. We paired groups A and B (made up of trios of teenagers) and groups C and D (made up of pairs) in a multiple baseline design per reading group, in which all groups went through the baseline conditions (BL), intervention (LuDiCa) and maintenance.

Results
LuDiCa increased the frequency of conversational acts of both autistic participants and neurotypical peers. In addition, the intervention favored initiations, questions, and sharing experiences, through the shared activity of reading and talking about a work of fiction. Participants rated the intervention in relation to the platform, the book, the reading facilitator, and interaction with peers. We discuss the potential of the facilitator's role in favoring interactions and the potential of LuDiCa as a joint activity for the engagement of adolescents. We also include suggestions for future research focused on the online context and discuss some limitations of the LuDiCa intervention.

Conclusion
In summary, our study offers initial experimental evidence demonstrating the positive impact of LuDiCa on social interaction behaviors among both autistic and neurotypical adolescents within an inclusive setting.
Recovery during Successive 120-min Football Games: Results from the 120-min Placebo/Carbohydrate Randomized Controlled Trial
Authors: ERMIDIS GEORGIOS , MOHR MAGNI , JAMURTAS ATHANASIOS Z. , DRAGANIDIS DIMITRIOS , POULIOS ATHANASIOS , PAPANIKOLAOU KONSTANTINOS , VIGH-LARSEN JEPPE F. , LOULES GEORGIOS , SOVATZIDIS APOSTOLOS , NAKOPOULOU THEOFANO , TSIMEAS PANAGIOTIS , DOUROUDOS IOANNIS I. , PAPADOPOULOS CONSTANTINOS , PAPADIMAS GIORGOS , ROSVOGLOU ANASTASIA , LIAKOU CHRISTINA , DELI CHARIKLIA K. , GEORGAKOULI KALLIOPI , CHATZINIKOLAOU ATHANASIOS , KRUSTRUP PETER , FATOUROS IOANNIS G. 
Published: February 2024

ABSTRACTPurposeThis study aimed to examine the recovery kinetics (i.e., time-dependent changes) of performance-related variables between two 120-min male football games performed 3 d apart with and without carbohydrate supplementation.MethodsTwenty male players (20 ± 1 yr; body fat, 14.9% ± 5.1%; maximal oxygen consumption, 59.4 ± 3.7 mL·kg−1·min−1) participated in two 120-min football games (G1, G2) according to a randomized, two-trial, repeated-measures, crossover, double-blind design. Participants received carbohydrate/placebo supplements during recovery between games. Field activity was monitored during the games. Performance testing and blood sampling were performed before and at 90 and 120 min of each game. Muscle biopsies were collected at baseline and at 90 and 120 min of G1 and pre-G2.ResultsCompared with G1, G2 was associated with reduced total distance (10,870 vs 10,685 m during 90 min and 3327 vs 3089 m during extra 30 min;P= 0.007–0.038), average (6.7 vs 6.2 km/h during extra 30-min game-play;P= 0.007) and maximal speed (32.2 vs 30.2 km/h during 90 min and 29.0 vs 27.9 km/h during extra 30 min;P< 0.05), accelerations/decelerations (P< 0.05), and mean heart rate (P< 0.05). Repeated sprint ability (P< 0.001), jumping (P< 0.05), and strength (P< 0.001) performance were compromised before and during G2. Muscle glycogen was not restored at G2 baseline (P= 0.005). Extended game-play reduced lymphocyte, erythrocyte counts, hematocrit, hemoglobin, reduced glutathione (P< 0.05) and increased delayed onset of muscle soreness, creatine kinase activity, blood glycerol, ammonia, and protein carbonyls (P< 0.05) before and during G2. Pax7+(P= 0.004) and MyoD+cells (P= 0.019) increased at baseline G2. Carbohydrate supplementation restored performance and glycogen, reduced glycerol and delayed onset of muscle soreness responses, and increased leukocyte counts and Pax7+and MyoD+cells.ConclusionsResults suggest that extended football games induce a prolonged recovery of performance, which may be facilitated by carbohydrate supplementation during a congested game fixture.
Ipsilateral lower limb motor performance and its association with gait after stroke
Authors: Lee Pei-Yun , Chen Chih-Hung , Tseng Hui-Yu , Lin Sang-I 
Published: February 2024

Background and purpose
Motor deficits of the ipsilateral lower limb could occur after stroke and may be associated with walking performance. This study aimed to determine whether the accuracy and movement path of targeted movement in the ipsilateral lower limb would be impaired in the chronic stage of stroke and whether this impairment would contribution to gait.

Methods
Twenty adults with chronic stroke and 20 age-matched controls went through Mini Mental Status Examination (MMSE), and a series of sensorimotor tests. The targeted movement tasks were to place the big toe ipsilateral to the lesion at an external visual target (EXT) or a proprioceptive target (PRO, contralateral big toe) with eyes open (EO) or closed (EC) in a seated position. A motion analysis system was used to obtain the data for the calculation of error distance, deviation from a straight path, and peak toe-height during the targeted movement tasks and gait velocity, step length, step width and step length symmetry of the lower limb ipsilateral to the brain lesion during walking.

Results
The stroke group had significantly lower MMSE and poorer visual acuity on the ipsilateral side, but did not differ in age or other sensorimotor functions when compared to the controls. For the targeted movement performance, only the deviation in PRO-EC showed significant between-group differences (p = 0.02). Toe-height in both EXT-EO and in PRO-EO was a significant predictor of step length (R2 = 0.294, p = 0.026) and step length symmetry (R2 = 0.359, p = 0.014), respectively.

Discussion and conclusions
The performance of ipsilateral lower limb targeted movement could be impaired after stroke and was associated with step length and its symmetry. The training of ipsilateral targeted movement with unseen proprioceptive target may be considered in stroke rehabilitation.
Oral post-treatment supplementation with a combination of glutamine, citrulline, and antioxidant vitamins additively mitigates jejunal damage, oxidative stress, and inflammation in rats with intestinal ischemia and reperfusion
Authors: Chiu Yu-Wen , Lee Chien-Hsing , Lo Hui-Chen 
Published: February 2024

IntroductionIntestinal ischemia and reperfusion (IIR) injury is closely associated with oxidative stress. Evidence shows that oral supplementation with glutamine and citrulline alleviates IIR-induced jejunal damage. We investigated the effects of a combination of glutamine, citrulline, and antioxidant vitamins on IIR-induced jejunal damage, oxidative stress, and inflammation.MethodMale Wistar rats that underwent 60 min of superior mesenteric artery occlusion were orally administered glutamine plus citrulline (GC), vitamin C plus E (CE), or a combination of GC and CE 15 min before and 3, 9, and 21 h after reperfusion. Healthy rats without IIR were used as controls.ResultsAfter reperfusion for 24 h, rats with IIR showed lower levels of red blood cells, hemoglobin, serum glucose, and jejunal DNA and increased white blood cell counts compared to controls (1-way ANOVA with the least significant difference,P< 0.05). The IIR-induced decrease in serum albumin and increase in plasma interleukin-6 and jejunal thiobarbituric acid-reactive substances (TBARS) were significantly reversed by GC and/or CE. The results of the 2-way ANOVA indicated that GC was the main factor that increased jejunal villus height and muscularis DNA, and CE was the main factor that increased jejunal muscularis protein and decreased jejunal proinflammatory cytokine levels and myeloperoxidase activity. In addition, GC and CE are the main factors that decrease plasma proinflammatory cytokine levels and the jejunal apoptotic index.ConclusionOral post-treatment supplementation with glutamine and citrulline, combined with vitamins C and E, may alleviate IIR-induced oxidative stress, inflammation, and jejunal damage.
Spontaneous Hepatic Hemorrhage Causing Acute Liver Failure Requiring Liver Transplant
Authors: Shukla Krunal , Chaudhari Rahul , Asgharpour Amon 
Published: February 2024

ABSTRACT
Spontaneous hepatic hemorrhage (SHH) is a very rare but life-threatening entity that results from a breach in the hepatic parenchyma without any external cause, the most common being hepatocellular carcinoma and hepatic adenoma. We present a case of SHH without any underlying tumor or injury. The cause in our patient remained unclear, but we hypothesize that the patient's SHH was most likely coagulopathy-related.
Ischemic Polypectomy Through Detachable Snare and Rubber Band Ligation in Peutz–Jeghers Syndrome
Authors: Tan Justin Ryan L. , Co Jonard T. 
Published: February 2024

ABSTRACTEndoscopic polypectomy is essential for the prevention of Peutz–Jeghers syndrome–associated complications, including intussusception, intestinal obstruction, and malignant transformation. Conventional polypectomy is the preferred approach, but it can be challenging to achieve in patients with Peutz–Jeghers syndrome because of the high polyp burden and polyps located in areas with difficult endoscopic access. This case report highlights 2 different techniques of ischemic polypectomy and its advantage compared with conventional polypectomy in this subset of patients.
Analisis Kualitas Perairan Berdasarkan Kelimpahan Dan Jenis Makrozoobentos Di Sungai Banjir Kanal Barat, Semarang
Authors: Hidayah Nurul , Ain Churun , Jati Oktavianto Eko 
Published: February 2024

Sungai Banjir Kanal Barat merupakan salah satu sungai di Semarang yang dimanfaatkan oleh masyarakat sekitar. Sungai ini termasuk dalam salah satu sungai besar di Kota Semarang. Adapun berbagai kegiatan di sepanjang aliran sungai ini yang menghasilkan limbah diantaranya adalah pemukiman penduduk (domestik), peternakan, pasar dan kegiatan perindustrian. Buangan limbah domestik dan non-domestik tersebut masuk ke dalam badan perairan hal ini akan memberi dampak terhadap kualitas perairan dan mempengaruhi makrozoobentos yang ada diperairan. Tujuan dari penelitian ini adalah untuk mengetahui kualitas perairan sungai dan mengetahui kelimpahan makrozoobentos. Penelitian ini menggunakan metode studi kasus dengan analisis deskriptif yang dilaksanakan pada bulan September 2022. Penentuan lokasi sampling menggunakan metode purposive sampling dengan total 3 stasiun. Sampel yang diambil adalah sedimen dan makrozoobenthos. Status kualitas perairan Sungai Banjir Kanal Barat dilihat dari nilai indeks keanekaragaman adalah perairan terindikasi tercemar berat. Keanekaragaman makrozoobenthos di perairan termasuk dalam kategori keanekaragaman rendah dengan nilai berkisar antara 0,49-0,57. Jenis makrozoobenthos yang ditemukan dikelompokkan dalam 2 kelas yaitu: Gastropoda (Melanoides punctata, Melanoides plicaria, Melanoides tuberculata, Terebia granifera, Bumilus tentaculata) dan Bivalvia (Anadara granosa).
Comparison of permeable cell culture inserts for use in culture of a human in vitro air–liquid interface model system
Authors: Brocke Stephanie A. , Speen Adam M. , Masood Syed , Worden Cameron P. , Jaspers Ilona 
Published: February 2024

AbstractIn this study, we compared 12 mm cell culture inserts with permeable polyester membranes (0.4 μm pores) from two different manufacturers: CELLTREAT® and Corning®. Physical dimensions and masses of the inserts were found to be very similar between the two brands, with CELLTREAT® inserts having a slightly smaller diameter and growth area (11.91 mm; 1.11 cm2) compared to Corning® Transwells® (12 mm; 1.13 cm2). We compared cell differentiation outcomes of human nasal epithelial cells (HNECs) at air–liquid interface grown on inserts from the two different manufacturers, including trans‐epithelial electrical resistance, ciliary beat frequency, ciliated area, and gene expression. HNECs from three male donors were used for all endpoints. No statistically significant differences were observed between paired cultures grown on different brands of insert. In conclusion, these inserts are comparable for use with airway epithelial cell model systems and likely do not impact cellular differentiation or cell culture quality.
Sexual and Reproductive Health Outcomes Among Adolescent Females During the COVID-19 Pandemic
Authors: Vandermorris Ashley , Toulany Alene , McKinnon Britt , Tam Michelle W. , Li Zhiyin , Guan Jun , Stukel Thérèse , Fu Longdi , Wang Xuesong , Begun Stephanie , Harrison Megan E. , Wigle Jannah , Brown Hilary K. 
Published: February 2024

BACKGROUND AND OBJECTIVES
Coronavirus disease 2019 (COVID-19) posed a significant threat to adolescents’ sexual and reproductive health. In this study, we examined population-level pregnancy and sexual health-related care utilization among adolescent females in Ontario, Canada during the pandemic and evaluated relationships between these outcomes and key sociodemographic characteristics.

METHODS
This was a population-based, repeated cross-sectional study of &gt;630 000 female adolescents (12–19 years) during the prepandemic (January 1, 2018–February 29, 2020) and COVID-19 pandemic (March 1, 2020–December 31, 2022) periods. Primary outcome was pregnancy; secondary outcomes were contraceptive management visits, contraception prescription uptake, and sexually transmitted infection (STI) management visits. Poisson models with generalized estimating equations for clustered count data were used to model pre-COVID-19 trends and forecast expected rates during the COVID-19 period. Absolute rate differences between observed and expected outcome rates for each pandemic month were calculated overall and by urbanicity, neighborhood income, immigration status, and region.

RESULTS
During the pandemic, lower-than-expected population-level rates of adolescent pregnancy (rate ratio 0.87; 95% confidence interval [CI]:0.85–0.88), and encounters for contraceptive (rate ratio 0.82; 95% CI:0.77–0.88) and STI management (rate ratio 0.52; 95% CI:0.51–0.53) were observed. Encounter rates did not return to pre-pandemic rates by study period end, despite health system reopening. Pregnancy rates among adolescent subpopulations with the highest pre-pandemic pregnancy rates changed least during the pandemic.

CONCLUSIONS
Population-level rates of adolescent pregnancy and sexual health-related care utilization were lower than expected during the COVID-19 pandemic, and below-expected care utilization rates persist. Pregnancy rates among more structurally vulnerable adolescents demonstrated less decline, suggesting exacerbation of preexisting inequities.
Las experiencias interculturales y su influencia en la enseñanza del inglés como lengua extranjera
Authors: Galarza Pucha Jairo Patricio , Estupiñán Lince María Lorena , Guevara Torres Pamela Victoria 
Published: February 2024

Esta investigación presenta las experiencias interculturales de los docentes de la Universidad Técnica Estatal de Quevedo y su influencia en la Enseñanza del Inglés como Lengua Extranjera. El objetivo general de este estudio es identificar cómo las experiencias interculturales de los docentes contribuyen en la enseñanza del inglés, determinando y describiendo vivencias interculturales tales como intercambios académicos, viajes e interacciones con culturas extranjeras a fin de conocer la manera en que aportan al desarrollo de la competencia intercultural en el aula. Se desarrolló una investigación descriptiva, de carácter mixto, con instrumentos cualitativos y cuantitativos como la entrevista y la encuesta. El análisis de los datos fue temático y de contenido. Se obtuvo que algunos docentes consideran que la experiencia intercultural que poseen les permite realizar actividades donde los alumnos pueden demostrar y aplicar los valores de la cultura extranjera. En cuanto a la competencia intercultural desarrollada en los estudiantes, la mayoría de los participantes hizo énfasis en llevar a los estudiantes a leer y revisar experiencias interculturales, ya sea, a través de los textos o de materiales, recursos y estrategias diversas como videos e interacciones con estudiantes extranjeros mediante videoconferencias. Lo que permite concluir que las experiencias interculturales influyen positivamente en la motivación y el compromiso de los docentes y estudiantes, al crear un ambiente de aprendizaje más estimulante para los estudiantes al oír y aprender de sus experiencias.
Burfoot, Annette & Güngör, Derya (2022). Women and Reproductive Technologies. The Socio-Economic Development of Technologies Changing the World
Authors: Otín-Gavín Jorge 
Published: February 2024

Este artículo reseña: Burfoot, Annette & Güngör, Derya (2022). Women and Reproductive Technologies. The Socio-Economic Development of Technologies Changing the World. New York, NY: Routledge.
FACTORS ASSOCIATED WITH THE OCCURRENCE OF PRIMARY MULTIDRUG-RESISTANT TUBERCULOSIS IN MEDAN
Authors: Hutomo Rizky Aditya , Wahyuni Chatarina Umbul , Sarumpaet Sorimuda 
Published: February 2024

Background: Tuberculosis (TB) treatment failure may lead to multidrug-resistant tuberculosis (MDR-TB). This failure can be detrimental to patients, not only causing death, but can also be transmitted to others, and those who are infected directly fall into the category of MDR-TB so that it can be referred to as primary MDR-TB. MDR-TB is the biggest problem in the prevention and eradication of TB worldwide. MDR-TB is a type of Mycobacterium tuberculosis resistance to at least the first two TB drugs of choice, including Isoniazid and Rifampicin. Purpose: This study aimed to determine the association between education, contact history, and knowledge regarding the occurrence of primary MDR-TB. Methods: This study used a cross-sectional study conducted at Community Health Center in Medan from November 2021 to February 2022. A total of 47 TB patients were selected for this study through purposive sampling methods. The dependent variable was primary MDR-TB occurrence while the independent variables consisted of several characteristics, namely education, contact history, and knowledge. The data were analyzed using chi-square. Results: in this study, sex (p=0.56; PR=1.12; 95% CI= 0.30<1,12<4,14;) and education (p=0.40; PR=0.56; 95% CI= 0.14<0.56<2.19) was not associated, while age (p=0.02; PR=0.16; 95% CI=0.02<0.16<0.94), contact history (p<0.001; PR=7.94; 95% CI=2.11<7.94<29.83) and poor knowledge (p=0.01; PR=5.00; 95% CI= 1.31<5.00<18.96) were associated with an occurrence of primary MDR-TB. Conclusion: the prevalence of primary MDR-TB increases with age, contact history, and poor knowledge.
PREVALENCE OF MENSTRUAL MIGRAINE AND THE EFFICACY OF SUMATRIPTAN ADMINISTRATION
Authors: Maridsky Aldilla Afifie Putri , Sudibyo Devi Ariani , Qurnianingsih Ema , Dyah Kencono Wungu Citrawati 
Published: February 2024

Background: Menstrual migraine is a primary headache that often attacks women of reproductive age. This type of migraine has high severity and is resistant to treatment. Therefore, an effective treatment is needed to treat this menstrual migraine. Sumatriptan has been shown to have a better effect as an acute treatment for menstrual migraine patients. Purpose: This study aims to determine the prevalence and effectiveness of sumatriptan as a therapy for menstrual migraine. Methods: This research is based on a literature review following predetermined inclusion criteria. First, a literature search was carried out adhering to the PRISMA flow in 4 databases: Pubmed, Science Direct, ProQuest, and ClinicalTrial.co. Results: Based on keywords, 267 studies were found in the literature search. However, only three studies covering 845 participants met the predetermined inclusion criteria. The average age of menstrual migraine patients in the three studies was 37 years. These three studies of sumatriptan revealed a significant effect in providing a pain-free response in menstrual migraine patients. These three studies also showed that sumatriptan 100 mg has a higher level of effectiveness in providing a pain-free response in menstrual migraine sufferers and was preferably given during mild attacks. Conclusion: These three studies indicate that typically, women who suffer from menstrual migraines are of reproductive age. Additionally, this study shows that using sumatriptan as an acute therapy significantly helps provide a pain-free response in menstrual migraine patients.