bluuebunny/crossref_metadata_2025_split huggingface dataset:
Capillary electrochromatography of caffeine and its metabolites in rat brain microdialysate
Authors: Ohyama Kaname , Wada Mitsuhiro , Lord Gwyn A. , Ohba Yoshihito , Nakashima Mihoko N. , Nakashima Kenichiro , Akiyama Shuzo , Lim Chang Kee , Kuroda Naotaka 
Published: February 2005

AbstractA capillary electrochromatography (CEC) method has been developed for the separation of caffeine and its two metabolites 1‐methylxanthine (1‐MX) and 1,7‐dimethylxanthine (1,7‐DX). The stationary phase was 3‐(1,8‐naphthalimido) propyl‐modified silyl silica gel (NAIP) and the best separations were achieved with 4.0 mM citrate buffer (pH 5.0) containing 80% methanol at an applied voltage of 25 kV. The compounds were completely separated in less than 3.5 min with good repeatability, which was approximately 3‐times less than that in high‐performance liquid chromatography (HPLC) with NAIP. The proposed method coupled with microdialysis was successfully applied to the monitoring of caffeine concentration in rat brain with detection limits of 1.11 μg/mL.
Chiral ion‐exchange capillary electrochromatography of arylglycine amides with dextran sulfate as a pseudostationary phase
Authors: Chen Yi , Lu Xiaoning , Han Zhiqiang , Qi Li , Wang Mei Xiang , Yu Xiao , Yang Gengliang , Mao Lanqun , Ma Huiming 
Published: February 2005

AbstractA low‐cost tunable chiral ion‐exchange capillary electrochromatographic method has been developed for the separation of arylglycine amide racemic mixtures with dextran sulfate (DS) as an anionic and chiral pseudostationary phase and Tris‐tartrate as a buffer system. The concentrations of DS and Tris had opposite influences on retention and resolution and could serve as ideal factors to finely tune the running speed and chiral resolution. Tartrate and pH largely impact the separation but pH should be confined within 3.0–5.5, only suitable for coarse tuning, while tartrate was preserved as the key buffering reagent, normally maintained at 40 mmol/L. With a working system composed of 0.1–1.0% DS, 20–60 mmol/L Tris, and 40 mmol/L tartrate at pH 3.50–4.50, the enantioresolution of arylglycine amides was shown to be dependent on their chemical structure: The chiral resolution increased when the hydrogen at the α‐amino group or at the p‐position of phenyl ring was replaced by other larger group(s) but the resolution decreased when the group at the o‐ or m‐site on the phenyl ring was enlarged. Further, the electronegative substitute of ‐Cl had larger resolution increment than methyl or methoxy at the position p‐ of phenyl ring but much lower increment at position m‐. It is possible to well explain the resolution variation phenomenon by considering the group resistance and the variation of hydrogen‐bonds formed inside the amino amides and between the solutes and DS. The amido group was shown irreplaceable to have chiral resolution with DS alone as an ionic and chiral pseudostationary phase.
Combining solid‐phase microextraction and on‐line preconcentration‐capillary electrophoresis for sensitive analysis of pesticides in foods
Authors: Hernández‐Borges Javier , Cifuentes Alejandro , García‐Montelongo Francisco J. , Rodríguez‐Delgado Miguel Á. 
Published: February 2005

AbstractThe combined use of solid‐phase microextraction (SPME) and different on‐line preconcentration strategies for ultrasensitive capillary electrophoresis‐ultraviolet (CE‐UV) analysis of five pesticides in a single run is investigated. Normal stacking mode (NSM), field‐enhanced sample injection (FESI), and stacking with matrix removal (SWMR) are explored to increase the sensitivity of the CE‐UV analysis of a selected group of pesticides (cyprodinil, cyromazine, pyrifenox, pirimicarb, and pyrimethanil). It could be observed that reverse polarity‐stacking with matrix removal (RP‐SWMR) provided the best results in terms of sensitivity (enhancement was up to 272‐fold compared with normal injection). The separation buffer consisted of 0.4 mM cetyltrimethylammonium chloride (CTAC), 0.4 M acetic acid at pH 4 containing 5% v / v 2‐propanol. This approach was then combined with SPME to determine the pesticides in water, apple, and orange juice. The combination of both preconcentration procedures allowed the determination of these pesticides at concentrations down to 2.5 μg / L in water and 3.1 μg / L in juices (i.e., levels well below the maximum residue limits allowed for these compounds). To our knowledge, this is the first report showing the great possibilities of the combined use of SPME, on‐line sample preconcentration, and CE for pesticide analysis.
Psychosocial factors involved in delayed consultation by patients with head and neck cancer
Authors: Rozniatowski Olivier , Reich Michel , Mallet Yann , Penel Nicolas , Fournier Charles , Lefebvre Jean‐Louis 
Published: February 2005

AbstractBackground.In the north of France, a delay in primary consultation has been noted among patients with head and neck cancer. This group is often correlated with lower socioeconomic status and a lack of medical information. Therefore, the choice to seek consultation is often influenced by symptoms such as pain and change in the size of tumors in the neck. We studied this delay in seeking consultation, focusing on psychosocial variables such as professional and social background, the involvement of a spouse/partner, and the presence of anxiety and depression.Methods.Two rating scales were administered to 50 patients with large tumors (T3/T4) and 50 patients with small tumors (T1/T2), and the results were compared. These rating scales were as follows: (1) a 17‐item questionnaire assessing sociodemographic data, presenting symptoms, factors generating the consultation, and reasons for delay; and (2) the Hospital Anxiety and Depression Scale (HADS).Results.Both groups were predominantly male and working‐class. Significant differences were observed in time since symptom onset and in conscious delay in seeking medical attention. The group with large tumors was characterized by lower involvement of a spouse/partner, conscious delay before first consultation, greater social isolation, fewer medical visits, and lower HADS anxiety scores. The group with small tumors sought consultation sooner and was characterized by greater involvement of a spouse/partner, correlated with significant anxiety. Depression was not a factor influencing delay within either group.Conclusions.The interpersonal relationship with a spouse/partner seemed to be essential in the dynamics surrounding consultation. Anxiety, rather than socioeconomics status, was a discriminating factor in the delay in seeking consultation. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005
Metastatic head and neck carcinoma to a percutaneous endoscopic gastrostomy site
Authors: Adelson Robert Todd , Ducic Yadranko 
Published: February 2005

AbstractBackground.Percutaneous endoscopic gastrostomy (PEG) tube placement is a safe and widely accepted alternate route for enteral alimentation in the head and neck cancer patient population. Cancer metastatic to a PEG tube exit site is a rare but serious complication of this procedure. We sought to determine the route of spread responsible for PEG site metastases such that we may prevent further occurrences of this highly morbid condition. We also report a case of PEG site metastasis at our institution.Methods.We performed a MEDLINE search for the years 1962 to 2002 and conducted a review of the literature. In the case at our institution, a 63‐year‐old man was referred to our institution with recurrent squamous cell carcinoma of the right base of tongue; he also had a 1.5‐cm left apical lung nodule. He underwent PEG tube placement at the time of staging panendoscopy. Six months after the original tube placement, he had an ulcerated mass develop at the PEG site; biopsy of the mass revealed squamous cell carcinoma histologically identical to the base of tongue tumor. He also had recurrent lung cancer and four hepatic lesions develop.Results.In our MEDLINE search, of the five patients diagnosed with PEG site disease >10 months after PEG placement, all five (100%) had synchronous distant metastatic disease. In the group of patients diagnosed with PEG site metastases ≤10 months after PEG placement, only four (24%) of 17 had synchronous distant metastatic disease. All patients underwent PEG placement by means of the “pull” technique. Direct implantation with a variable‐sized initial tumor burden can explain all cases of PEG site metastasis. The presence of distant metastases is representative of the natural history of advanced head and neck malignancies. Smaller initial tumor implants present later than would larger initial tumor burdens, when the patient is more likely to have distant metastatic disease. In the case at our institution, the patient did not respond to treatment for his hepatic and PEG site metastases and his lung cancer, and he died 4 months after detection of the PEG site metastasis.Conclusions.PEG site metastases are iatrogenic complications of PEG tube placement in patients with squamous cell carcinoma of the upper aerodigestive tract. The use of laparoscopic, open, or the “push” technique of PEG tube placement in patients with head and neck cancer may prevent direct implantation of malignant cells into an enteral access site. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005
Oncologic effectiveness of selective neck dissection in the N0 irradiated neck
Authors: Solares C. Arturo , Fritz Michael A. , Esclamado Ramon M. 
Published: February 2005

AbstractBackground.The incidence and patterns of nodal spread in previously irradiated N0 necks are not well defined. Therefore, the safety and efficacy of selective neck dissection (SND) in this patient population is not well established. In a previous report from our institution, SND in irradiated patients with recurrent disease at the primary site but clinically negative necks resulted in excellent tumor control in the neck. The objective of this study is to validate our initial observations in a larger sample of patients with longer follow‐up.Methods.A retrospective chart analysis of patients previously treated with primary radiation therapy or chemoradiotherapy for squamous cell carcinoma (SCC) of the head and neck between January 1997 and June 2003 was performed. Patients with recurrent or persistent disease at the primary site or a second primary head and neck SCC, with no clinical or radiologic disease in the neck, who underwent surgical salvage with resection of the primary site along with a site‐specific SND were analyzed. Patients who remained disease free at the primary site were analyzed for regional control after SND.Results.Sixty‐nine patients underwent a total of 96 site‐specific SNDs. The mean age was 64.1 years (range, 39–91 years). There was histologically positive nodal disease in 17 of 69 patients (25%), and 22 of 96 necks (23%). Fifty‐three patients had at least a 12‐month follow‐up. The mean follow‐up was 23.3 months (range, 1–96 months). Of the patients with 1‐year of follow‐up, six patients died from recurrence at the primary site, and nine died from distant metastasis. There were no cases of neck recurrence with the primary site controlled. All patients who had more than two positive nodes had recurrence either at the primary site or distant metastasis.Conclusions.Our results confirm that the patterns of lymphatic spread are not affected by radiation. We conclude that SND is oncologically safe in the management of the N0 irradiated neck and that the finding of more than two positive nodes predicts a poor outcome. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005
Quality of life as predictor of weight loss in patients with head and neck cancer
Authors: Petruson Karin M. , Silander Ewa M. , Hammerlid Eva B. 
Published: February 2005

AbstractBackground.The purpose of this prospective study was to evaluate whether a relationship exists between malnutrition (≥10% weight loss) and health‐related quality of life (HRQL) in patients with head and neck cancer and whether weight loss can be predicted with HRQL questionnaires.Methods.Weight and HRQL were monitored in 49 patients. HRQL was assessed longitudinally, using the European Organization for Research and Treatment of Cancer Quality of Life Core Questionnaire (EORTC QLQ‐C30), the EORTC Head and Neck Cancer module (QLQ‐H&N35), and the Hospital Anxiety and Depression Scale (HADS).Results.At diagnosis, those patients who had a weight loss greater than 10% (n = 20) after treatment scored significantly worse on 15 of 28 HRQL variables than did patients who lost less (n = 29). The largest difference (Δ ≥20) was found for role functioning, fatigue, loss of appetite, global quality of life, sticky saliva, and swallowing. Differences in HRQL persisted even after 3 years. The fatigue scale was the only significant predictor of weight loss (p = .005) at diagnosis.Conclusions.Patients with head and neck cancer who are at risk of severe weight loss developing during treatment may be detected with the aid of HRQL questionnaires at diagnosis. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX,2005
Congenital intracranial frontotemporal dermoid cyst presenting as a cutaneous fistula
Authors: Scolozzi Paolo , Lombardi Tommaso , Jaques Bertrand 
Published: February 2005

AbstractBackground.Intracranial extension and a cutaneous sinus tract are rarely seen with craniofacial dermoid cysts, with few cases reported in the literature.Methods.We report a case of a 1‐year‐old girl who was initially seen with a cutaneous fistula of the frontotemporal region, which revealed an intracranial dermoid cyst.Results.The patient underwent a right lateral orbitotomy by a bicoronal approach. The cyst was seated within the lateral orbital wall, with intracranial extension through the temporal and sphenoidal bones to the dura of the temporal lobe. Histopathologic analysis confirmed the diagnosis of a dermoid cyst.Conclusions.Craniofacial dermoid cysts may be associated with a cutaneous sinus tract and/or intracranial extension. Failure to recognize and promptly treat these lesions may lead to a progressive skeletal distortion and/or recurrent infection with a potential for meningitis or cerebral abscess. Therefore, detailed CT and MRI scans are mandatory before surgical treatment of any cutaneous fistula in the head and neck region. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005
Role of phosphorylated CaMKII and calcineurin in the differential effect of hypothyroidism on LTP of CA1 and dentate gyrus
Authors: Gerges N.Z. , Alzoubi K.H. , Alkadhi K.A. 
Published: February 2005

AbstractHypothyroidism impairs early long‐term potentiation (LTP) in the CA1 but not in the dentate gyrus (DG) of hippocampus of anesthetized adult rats. Protein levels and activities of signaling molecules in both the CA1 and DG of surgically thyroidectomized and sham‐operated euthyroid rats were measured. Basal levels of total calmodulin kinase II (CaMKII) protein in both the CA1 and DG were decreased in hypothyroidism. Marked reduction of basal P‐CaMKII levels and CaMKII activity was seen in CA1, but not in the DG of the same hypothyroid animals. Basal levels of calmodulin and protein kinase Cγ (PKCγ) were decreased in CA1 but remained unchanged in the DG of hypothyroid rats. Basal calcineurin levels and activity, although enhanced in CA1, were reduced in the DG of hypothyroid rats. These findings suggest that the DG may possess a compensatory mechanism whereby calcineurin levels are reduced, to allow sufficient CaMKII activity to produce an apparently normal LTP in hypothyroid rats. © 2005 Wiley‐Liss, Inc.
Preparation and characterization of novel tricalcium silicate bioceramics
Authors: Zhao Wenyuan , Chang Jiang 
Published: February 2005

AbstractNovel tricalcium silicate (Ca3SiO5) ceramics were successfully fabricated. The mechanical properties of the ceramics were dependent remarkably on sintering temperature. The fracture toughness, Young's modules, and bending strength of Ca3SiO5 ceramics sintered at 1500°C were 1.93 MPa · m1/2, 36.7 GPa, and 93.4 MPa, respectively. These findings suggest that the Ca3SiO5 ceramics possess good mechanical properties, and might be a potential bone implant material. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 86–89, 2005
Biocompatibility of titanium implants modified by microarc oxidation and hydroxyapatite coating
Authors: Li Long‐Hao , Kim Hae‐Won , Lee Su‐Hee , Kong Young‐Min , Kim Hyoun‐Ee 
Published: February 2005

AbstractA thin hydroxyapatite (HA) layer was coated on a microarc oxidized titanium (MAO‐Ti) substrate by means of the sol–gel method. The microarc oxidation (anodizing) enhanced the biocompatibility of the Ti, and the bioactivity was improved further by the sol–gel HA coating on the anodized Ti. The HA sol was aged fully to obtain a stable and phase‐pure HA, and the sol concentration was varied to alter the coating thickness. Through the sol–gel HA coating, the Ca and P concentrations in the coating layer increased significantly. However, the porous morphology and roughness of the MAO‐Ti was altered very little by the sol–gel treatment. The proliferation and alkaline phosphatase (ALP) activity of the osteoblast‐like cells on the MAO/HA sol–gel‐treated Ti were significantly higher than those on the MAO‐Ti without the HA sol–gel treatment. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 48–54, 2005
Adsorption of serum fetuin to hydroxylapatite does not contribute to osteoblast phenotype modifications
Authors: Xie Jianwei , Baumann Melissa J. , McCabe Laura R. 
Published: February 2005

AbstractOsteoblasts exhibit enhanced differentiation and altered gene profiles when cultured on hydroxyapatite (HA) compared to plastic surfaces. To begin determining mechanisms for this response, we used proteomics to identify proteins predominantly found in osteoblasts on HA but not plastic surfaces. Two‐dimensional gel electrophoresis and Western analyses indicate that fetuin is abundant in extracts from HA, but not plastic surfaces. Incubation of HA and plastic surfaces with cell culture medium (containing 10% serum) under cell‐free conditions shows that fetuin is predominantly derived from the culture medium serum and readily adsorbs to the HA surface. However, we did detect low levels of fetuin B mRNA in osteoblasts. Serum albumin, actin‐β, apolipoprotein‐AI, and vimentin also adsorbed to HA. To determine the role of fetuin in the HA‐induced osteoblast phenotype changes, osteoblasts were seeded onto fetuin‐coated or uncoated HA under serum‐free conditions. Osteoblast morphology was similar on both HA surfaces, suggesting that HA alone (without adsorbed serum proteins) is sufficient for cell attachment and spreading. Similarly, genes previously reported to be modulated by HA (glvr‐1, DMP‐1, osteoglycin, and proliferin 3) were modulated even in the absence of fetuin or other serum proteins. These data show that HA surface can be enriched selectively with fetuin from serum; however, neither fetuin or other serum proteins are required to mediate HA‐induced osteoblast attachment, spreading, or changes in expression of genes examined. This finding suggests that factors intrinsic to HA are required for the response. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 39–47, 2005
Flow cytometric measurement of phagocytosis reveals a role for C3b in metal particle uptake by phagocytes
Authors: Baldwin L. , Flanagan B. F. , Hunt J. A. 
Published: February 2005

AbstractA methodology for the quick and efficient study of phagocytosis has been developed. It uses the flow cytometer to exploit the change in size and granularity that occurs in cells upon the ingestion of particulate material. The numbers of cells that have phagocytosed particles can be calculated from the distinct shift in regions that occurs. The method also allows the factors governing phagocytosis to be studied in detail through the use of blocking agents or antibodies. Blood‐derived monocytes were studied to investigate the role of complement in metal particle phagocytosis to further understand aseptic loosening. Factor C3b was found to be fundamental to the opsonization and phagocytosis of metal particles by monocytes. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 80–85, 2005
Effect of particulate bioactive glasses on human macrophages and monocytes in vitro
Authors: Day Richard M. , Boccaccini Aldo R. 
Published: February 2005

AbstractBioactive glasses, originally developed to promote tissue adhesion, are finding an increasing array of biomedical applications. The aim of the current study was to assess the ability of silicate‐ and zinc phosphate‐based bioactive glasses to modulate the secretion of cytokines from activated human macrophages and monocytes. Human macrophages and monocytes were isolated and cultured on surfaces coated with a range of quantities of the bioactive glasses. Nontoxic concentrations of the glasses were selected and assessed further for their ability to modulate the secretion of tumor necrosis factor (TNF)‐α, interleukin (IL)‐10 and ‐6, in the presence or absence of the stimulant lipopolysaccharide. 45S5 glass produced a significant reduction to the amount of TNF‐α (p < 0.05) and IL‐6 (p < 0.01) secreted by stimulated cells compared with cells stimulated in the absence of bioactive glass. A significant reduction in IL‐6 secretion was also observed with the other silicate‐ and zinc phosphate‐based glasses tested. IL‐10 secretion was increased (but not significantly) in presence of all glasses tested. TNF‐α and IL‐6 secretion from stimulated cells was lower in presence of the silicate glasses compared with the zinc phosphate glasses, indicating that this system of bioactive glass might be of clinical use in conditions associated with inflammation. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 73–79, 2005
Metabolism of xanthohumol and isoxanthohumol, prenylated flavonoids from hops (Humulus lupulus L.), by human liver microsomes
Authors: Nikolic Dejan , Li Yongmei , Chadwick Lucas R. , Pauli Guido F. , van Breemen Richard B. 
Published: February 2005

AbstractThe female flowers of hops (Humulus lupulus L.) used to flavor beer contain the prenylated flavonoids xanthohumol (XN) and isoxanthohumol (IX). IX is moderately estrogenic in vitro and XN has pharmacological properties that might make it useful as a cancer chemopreventive agent. The metabolism of these dietary flavonoids was investigated in vitro using human liver microsomes. Hydroxylation of a prenyl methyl group was the primary route of oxidative metabolism forming either cis or trans hydroxylated metabolites of IX but only the trans isomer of XN. The double bond on the prenyl group of both compounds formed an epoxide which was opened by an intramolecular reaction with the neighboring hydroxyl group. The potent phytoestrogen 8‐prenylnaringenin (8‐PN) was detected as a demethylation product of IX. However, the analogous demethylation reaction was not observed for XN. Since XN can be converted to IX through acid‐catalyzed cyclization in the stomach, XN might contribute to the in vivo levels of estrogenic 8‐PN following consumption of hops extracts. Copyright © 2005 John Wiley & Sons, Ltd.