bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors Associated With the Dietary Patterns of Brazilian Adolescents: Analysis of the 2019 National School Health Survey
Authors: Silva Alanna Gomes da , Silva Thales Philipe Rodrigues da , Malta Deborah Carvalho 
Published: December 2023

Abstract
Background
during adolescence there are physical, psychic, and social changes that influence feeding behaviors and lifestyle, which can contribute to the development of several diseases, including obesity. This study aimed to identify the food consumption patterns of Brazilian adolescents and to verify their associated factors.
Methods
Cross-sectional study with data from the 2019 National School Health Survey (PeNSE). Students aged 13 to 17 participated in the survey, totaling a sample of 125,123 adolescents. Principal component analysis was used to identify the feeding behavior patterns. To evaluate the association, logistic regression was used, estimated by the Odds Ratio, with the respective 95% confidence intervals.
Results
Two main components were identified, the first related to a healthy food consumption pattern (regular consumption of fruits, vegetables, and beans) and the second to a mixed pattern (non-regular consumption of sweets, soft drinks, and food from snack bars). As for the associated factors, the adolescents most likely to belong to component 1 lived in the Midwest and Southeast regions, their mothers had higher education, and they had healthier lifestyle habits. Adolescents who belonged to component 2, on the other hand, were positively associated with being male, aged 16 and 17, studying in public schools, not consuming alcoholic beverages, and not being sedentary.
Conclusions
This study evidenced the formation of two food consumption patterns and both were associated with socioeconomic variables and healthy lifestyle habits. The identification of food consumption patterns among adolescents enables health surveillance and the strengthening of policies aimed at the school environment, with a view to reducing diseases and health problems.
Lentiviral mediated delivery of CRISPR/Cas9 reduces intraocular pressure in a mouse model of myocilin glaucoma
Authors: Patil Shruti V. , Kaipa Balasankara Reddy , Ranshing Sujata , Sundaresan Yogapriya , Millar J. Cameron , Nagarajan Bhavani , Kiehlbauch Charles , Zhang Qihong , Jain Ankur , Searby Charles C. , Scheetz Todd E. , Clark Abbot F. , Sheffield Val C. , Zode Gulab S. 
Published: December 2023

Abstract
Mutations in myocilin (MYOC) are the leading known genetic cause of primary open-angle glaucoma, responsible for about 4% of all cases. Mutations in MYOC cause a gain-of-function phenotype in which mutant myocilin accumulates in the endoplasmic reticulum (ER) leading to ER stress and trabecular meshwork (TM) cell death. Therefore, knocking out myocilin at the genome level is an ideal strategy to permanently cure the disease. We have previously utilized CRISPR/Cas9 genome editing successfully to target MYOC using adenovirus 5 (Ad5). However, Ad5 is not a suitable vector for clinical use. Here, we sought to determine the efficacy of adeno-associated viruses (AAVs) and lentiviruses (LVs) to target the TM. First, we examined the TM tropism of single-stranded (ss) and self-complimentary (sc) AAV serotypes as well as LV expressing GFP via intravitreal (IVT) and intracameral (IC) injections. We observed that LV_GFP expression was more specific to the TM injected via the IVT route. IC injections of Trp-mutant scAAV2 showed a prominent expression of GFP in the TM. However, robust GFP expression was also observed in the ciliary body and retina. We next constructed lentiviral particles expressing Cas9 and guide RNA (gRNA) targeting MYOC (crMYOC) and transduction of TM cells stably expressing mutant myocilin with LV_crMYOC significantly reduced myocilin accumulation and its associated chronic ER stress. A single IVT injection of LV_crMYOC in Tg-MYOCY437H mice decreased myocilin accumulation in TM and reduced elevated IOP significantly. Together, our data indicates, LV_crMYOC targets MYOC gene editing in TM and rescues a mouse model of myocilin-associated glaucoma.
AhR ligands from LGG metabolites promote piglet intestinal ILC3 activation and IL-22 secretion to inhibit PEDV infection
Authors: Wang Junhong , Zhao Yibo , Cui Tong , Bao Hongyu , Gao Ming , Cheng Mingyang , Sun Yu , Lu Yiyuan , Guan Jiayao , Zhang Di , Jiang Yanlong , Huang Haibin , Shi Chunwei , Wang Jianzhong , Wang Nan , Hu Jingtao , Yang Wentao , Yang Guilian , Zeng Yan , Wang Chunfeng , Cao Xin 
Published: December 2023

Abstract
In maintaining organismal homeostasis, gut immunity plays a crucial role. The coordination between the microbiota and the immune system through bidirectional interactions regulates the impact of microorganisms on the host. Our research focused on understanding the relationship between substantial changes in jejunal intestinal flora and metabolites and intestinal immunity during porcine epidemic diarrhea virus (PEDV) infection in piglets. We discovered that Lactobacillus rhamnosus GG (LGG) could effectively prevent PEDV infection in piglets. Further investigation revealed that LGG metabolites interact with type 3 innate lymphoid cells (ILC3) in the jejunum of piglets through the aryl hydrocarbon receptor (AhR). This interaction promotes the activation of ILC3 cells and the production of interleukin-22 (IL-22). Subsequently, IL-22 facilitates the proliferation of IPEC-J2 cells and activates the STAT3 signaling pathway, thereby preventing PEDV infection. Moreover, the AhR receptor exerts its influence on various cell types within organoids, including intestinal stem cells (ISCs), Paneth cells, and enterocytes, fostering their growth and development, suggesting a broad impact of AhR on intestinal health. In conclusion, our study demonstrates the ability of LGG to modulate intestinal immunity and effectively prevent PEDV infection in piglets. These findings highlight the potential application of LGG as a preventive measure against viral infections in livestock.
Reidentification of the causal agent and addition of new symptoms of black leaf blight in fig tree in Japan
Authors: Kikuhara Kenji , Inoue Kouji , Motohashi Kenichi 
Published: December 2023

Abstract
In August 2007, twig blight, branch canker, dieback, and fruit rot were observed in fig trees (cv. Toyomitsuhime) with black leaf blight in Japan. All isolates from each symptomatic part were identified as the fungi causing this disease. These symptoms were reproduced after inoculating their isolates; isolates were then reisolated. We demonstrated this fungus caused new symptoms. The isolates were re-evaluated based on morphological characteristics and phylogenetic analysis of concatenated rDNA-ITS, EF1-α, TUB2, and RPB2 partial sequences. They were phylogenetically classified as a new independent clade. We propose a new species of the fungus as Neofusicoccum ficicola.
Development of a nomogram to predict hemorrhage transformation after mechanical thrombectomy in patients with acute ischemic stroke caused by large vessel occlusion in the anterior circulation
Authors: Li Yuanhao , Yang Dongliang , Li Jianhua , Xia Rui , Wen Chao , Yang Suosuo , Kang Jian , Li Yuling 
Published: December 2023

Abstract

Background: Hemorrhage transformation (HT) is one of the serious complications after mechanical thrombectomy (MT) in patients with acute ischemic stroke (AIS) and is associated with poor prognosis. The aim of this study was to develop a nomogram to predict the risk of post-MT HT in AIS patients.
Methods: AIS patients treated with MT between January 2020 and June 2022 were enrolled in this study. The enrolled patients were randomly divided into training and validation cohorts, in a 2:1 ratio. Lasso regression and machine learning algorithms were used for feature selection. Multivariate logistic analysis was applied to identify the optimal predictors. A nomogram was developed to predict the risk of post-MT HT. Performance of the nomogram was determined by its discrimination, calibration, and clinical usefulness.
Results:A total of 205 patients were enrolled in the study, with 145 in the training cohort and 60 in the validation cohort. The common risk factors revealed by Lasso regression and random forest algorithm were blood glucose on admission, CRP, NIHSS score, ASPECTS and CRP-to-albumin ratio. Multivariate logistic analysis showed that blood glucose on admission (Odds Ratio (OR)=5.61, 95%CI:1.86-20.83, P=0.004) and CRP (OR=73.52, 95%CI: 25.06-276.77, P<0.001) were independent predictors of post-MT HT. The nomogram was developed based on blood glucose on admission and CRP. Moreover, the proposed nomogram showed good discriminative ability with an area under the curve of 0.924 and 0.846 in the training and validation cohort, respectively. The calibration plot showed good concordance between nomogram prediction and actual observation. Decision curve analysis indicated that the nomogram had favorable clinical application benefits.
Conclusion: This study proposed a nomogram based on CRP and blood glucose on admission to predict the risk of post-MT HT in anterior AIS patients. The nomogram showed reliable predictive performance and can help clinicians identify patients at high risk of HT.
Consumer wearables data impact pediatric surgery clinicians’ remote management
Authors: Carter Michela , Linton Samuel C. , Zeineddin Suhail , Pitt J. Benjamin , De Boer Christopher , Figueroa Angie , Gosain Ankush , Lanning David , Lesher Aaron , Islam Saleem , Sathya Chethan , Holl Jane L. , Ghomrawi Hassan M.K. , Abdullah Fizan 
Published: December 2023

Abstract
Purpose Integration of consumer wearables data has the potential to inform clinicians’ remote assessment of postoperative patients. This multi-institutional study evaluated the impact of adding objective recovery data from consumer wearables to postoperative telephone encounters on clinicians’ management.Methods Three telephone scenarios of post-appendectomy patients were presented to clinicians at five children’s hospitals. Each scenario was then supplemented with wearable data concerning for or reassuring against postoperative complication. Clinicians rated likelihood of ED referral and confidence in decision-making.Results Thirty-four clinicians participated. Compared to the scenario alone, the addition of reassuring wearable data resulted in decrease in likelihood of ED referral for all three scenarios (p < 0.01). When presented with concerning wearable data, there was significant increase in the likelihood of ED referral for two of three scenarios (p = 0.72, p = 0.02, p < 0.001). With the addition of wearable data, 76–88% of clinicians reported increased confidence in their recommendations.Conclusion The addition of wearable data to simulated telephone scenarios for post-discharge pediatric surgery patients impacted clinicians’ remote patient management and increased clinician confidence. Wearable devices are capable of providing real-time measures of recovery, which can be employed as a post-operative monitoring tool to reduce delays in care and avoidable health care utilization.
The effects of low HER2 expression on survival in patients with metastatic breast cancer treated with CDK 4/6 inhibitors: A multicentre retrospective study
Authors: Guliyev Murad , Şen Gülin Alkan , Gültürk İlkay , Majidova Nargiz , Akdağ Goncagül , Ahadzade Ali , Turna Hande , Demirci Nebi Serkan 
Published: December 2023

Abstract
Purpose: Endocrine therapy (ET) in combination with CDK 4/6 inhibitors (CDK 4/6i) is the standard treatment modality for hormone receptor (HR)-positive and HER2-negative metastatic breast cancer (mBC). For some authors, patients with low HER2 expression are considered a different subgroup. In this study, we aimed to investigate the prognostic effect of HER2 expression status on survival in mBC patients treated with first-line ET plus CDK 4/6i.
Methods: This multicenter retrospective study included patients with HR+/HER2-negative mBC cancer who were treated with first-line CDK 4/6i in combination with ET from January 2016 and March 2023. Patients were divided into two groups (HER2-low and zero), and survival and safety analyses were performed.
Results: A total of 201 patients were included in this study; of these, 73 (36.3%) had HER2-low disease and 128 (63.7%) had HER2-zero. There were 136 patients (67.2%) treated with ribociclib and 66 (32.8%) with palbociclib. Most of the patients (75.1%) received aromatase inhibitors as endocrine therapy. Baseline characteristics were similar between the two groups. The median follow-up was 19.1 months (range: 2.5–78.4). The most common side effect was neutropenia (22.4%). The frequency of grade 3–4 toxicity was similar between the HER2-zero and low patients (32% vs. 33.6%; p = 0.939). Visceral metastases were present in 44.8% of patients. Between the HER2-low and zero groups, median PFS (25.2 vs. 22.6 months, p = 0.972) and OS (not reached vs. 37.5 months, p = 0.707) showed no statistically significant differences.
Conclusion: The prognostic value of HER2-low status remains controversial. Our study showed no significant effect of HER2 low expression on survival in patients receiving CDK 4/6i plus ET.
The family caregiver needs assessment questionnaire for home hospice care of the dying elderly in Mainland China: Tool development and psychometric evaluation
Authors: Wang Lei , Li Yaru , Zhao Rui , Li Jiangxu , Gong Xiangru , LI Hongyu , Wang Ru 
Published: December 2023

Abstract
Background
As care moves from disease-centered to family-centered, meeting the needs of caregivers becomes more and more important. The existence of many unmet needs of family caregivers is mainly related to the lack of effective needs assessment tools. The goal of the study was to develop and validate a family caregiver needs assessment questionnaire for home hospice care of the dying elderly in Mainland China.
Method
Based on the guidance of Harmony Nursing Theory, 48 initial questionnaire items were initially drafted through literature review, reference to the maturity needs tool, interviews with 12 family caregivers of the dying elderly, and group discussions among research team members. Two rounds of expert review and small sample testing resulted in a 36-item prediction questionnaire. From May to August 2023, 223 family caregivers of the dying elderly in Jinzhou City, Liaoning Province were selected to verify the questionnaire. The validation process included (1) further screening of 34 items using item analysis and (2) psychometric techniques for reliability and validity analysis.
Results
The final questionnaire included 34 items. With a cumulative variance contribution rate of 71.281%, the exploratory factor analysis identified six common factors: emotional regulation needs, life care needs, end-of-life knowledge needs, social support needs, symptom control needs, and spiritual care needs. The questionnaire's total Cronbach coefficient was 0.910, and the retest reliability was 0.868. Its content validity index was 0.982, and the items' content validity index ranged from 0.83 to 1.00.
Conclusions
The questionnaire items compilation and screening methods are scientifically reliable, have strong reliability and validity. In the future, healthcare providers can utilize the questionnaire to fully assess the needs of family caregivers of elderly patients who are dying at home with hospice care.
Investigating changes in the premotor cortex-derived frontal-striatal-thalamic subcircuit in attenuated psychosis syndrome
Authors: Katagiri Naoyuki , Tagata Hiromi , Uchino Takashi , Arai Yu , Saito Junichi , Kamiya Kouhei , Hori Masaaki , Mizuno Masafumi , Nemoto Takahiro 
Published: December 2023

Abstract

Frontal-striatal-thalamic circuit impairment is presumed to underlie schizophrenia. Individuals with attenuated psychosis syndrome (APS) show longitudinal volume reduction of the putamen in the striatum, which has a neural connection with the premotor cortex through frontal-striatal-thalamic subcircuit. However, comprehensive investigations into the biological changes in the frontal-striatal-thalamic subcircuit originating from the premotor cortex are lacking in APS. We investigated differences in fractional anisotropy (FA) values between the striatum and premotor cortex (ST-PREM) and between the thalamus and premotor cortex (T-PREM) in individuals with APS and healthy controls, using a novel method TractSeg. Our study comprised 36 individuals with APS and 38 healthy controls. There was a significant difference between the control and APS groups in the right T-PREM (Odds ratio = 1.76, p = 0.02). Other factors, such as age, sex, other values of FA, and antipsychotic medication, were not associated with differences between groups. However, while FA value reduction of ST-PREM and T-PREM in schizophrenia has been previously reported, in the present study on APS, the alteration of the FA value was limited to T-PREM in APS. This finding suggests that ST-PREM impairment is not predominant in APS but emerges in schizophrenia. Impairment of the neural network originating from the premotor cortex can lead to catatonia and aberrant mirror neuron networks that are presumed to provoke various psychotic symptoms of schizophrenia. Our findings highlight the potential role of changes in a segment of the frontal-thalamic pathway derived from the premotor cortex as a biological basis of APS.
Long noncoding RNA C-Terminal binding protein 1 antisense regulates ovarian granulosa cells proliferation and autophagy and participates in polycystic ovary syndrome
Authors: Sun Kaixuan , song Jianbo , Xiu Yinling , Yu Tingting , Zhao Mengsi , Yu Yuexin 
Published: December 2023

Abstract

Long non-coding RNA (lncRNA) C-Terminal binding protein 1 antisense (CTBP1-AS) was reported that it is associated with polycystic ovarian syndrome (PCOS) in Chinese population and established the possibility that abnormal CTBP1-AS expression is a risk factor for PCOS, while the role of LncRNA CTBP1-AS in PCOS is not very clear. We speculated that lncRNA CTBP1-AS can regulates ovarian granulosa cells proliferation and autophagy and participates in polycystic ovary syndrome. This study was therefore carried out to explore its role in PCOS. In this study, 40 patients with PCOS and 40 controls were enrolled. The expression of lncRNA CTBP1-AS were analyzed by qRT-PCR. The direct interaction between lncRNA CTBP1-AS and proliferation was explored with Cell Counting Kit-8(CCK8), Colony formation and Edu assay experiments. The qRT-PCR and Western blotting assay was used to detect the lncRNA CTBP1-AS effect on the expression of autophagy-related proteins LC3 and p62. The results showed that LC3-II/I and p62 expressions significantly increased and decreased, respectively, after lncRNA CTBP1-AS overexpression in ovarian granulosa cells. After knocking down the lncRNA CTBP1-AS expression in ovarian granulosa cells, LC3-II/I and p62 expressions significantly decreased and increased, respectively. These results suggest that lncRNA CTBP1-AS can promote autophagy of ovarian granulosa cells. The lncRNA CTBP1-AS expression in PCOS patients significantly increased, and lncRNA CTBP1-AS could promote the proliferation of ovarian granulosa cells and the level of autophagy.
CLEP-GAN: An Innovative Approach to ECG Reconstruction from PPG Signals
Authors: Li Xiaoyan , Xu Shixin , Habib Faisal , Aminnejad Neda , Gupta Arvind , Huang Huaxiong 
Published: December 2023

Abstract
This study aims to advance the domain of zero-shot ECG reconstruction from PPG signals, addressing the limitations of existing methods that predominantly rely on future reference ECG subsets. We present two pivotal innovations: First, an end-to-end deep learning model for ECG reconstruction from PPG, which harmoniously integrates contrastive learning, adversarial learning, and attention gating. Second, we introduce a synthetic data generation technique using an ODE model, creating ECG-PPG pairs that serve as an additional dataset. Both synthetic and real-world datasets were employed to evaluate the efficacy of our models. Initial tests on the synthetic data yielded near-flawless reconstructions, while evaluations on real-world datasets highlighted the superior performance of our approach over established methodologies. Importantly, our findings emphasize the crucial role of factors, such as sex, in ECG reconstructions, suggesting that indiscriminate dataset augmentation might affect accuracy adversely. This research not only pioneers ECG reconstruction methods but also accentuates the significance of prudent dataset selection, offering profound implications for non-invasive cardiac monitoring advancements.
Sporosarcina siccatus sp. nov., isolated from coastal sediment of the Great Wall Station, Antarctica
Authors: Mu Hongmei , Xie Yuchun , Jiang Peiqiang , Liu Lukuan , Li Jing 
Published: December 2023

Abstract

A Gram-stain-positive, aerobic and spherical bacterium, white, slightly convex colony with rounded edges, designated GW1-11T was isolated from the coastal sediment of the Great Wall Station, Antarctica. 16S rRNA gene sequence analysis showed that strain GW1-11T was closely related to Sporosarcina ureae DSM 2281T (94%). Growth was observed in the presence of 0–6.0%(w/v) NaCl, at pH 6.0–9.0, and at 4–37℃. Digital DNA-DNA relatedness between strain GW1-11T and its close relatives S. ureae DSM 2281T was 21.0%. The average nucleotide identity value between strain GW1-11T and S. ureae DSM 2281T was 77.9%. The strain GW1-11T possessed menaquinone-7 (MK-7) as the sole respiratory quinone. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The major fatty acids composition was summarized as C14: 0, C14:0 ω5c and iso-C14: 0. Based on phenotypic, chemotaxonomic characteristics, and phylogenetic data, strain GW1-11T represents a novel species of the genus Sporosarcina, for which the name Sporosarcina siccatus sp. nov., is proposed. The type strain is GW1-11T (= CCTCC AB 2019391T = KCTC43187T).
Computational methods for model reduction in large-scale MIMO dynamical systems
Authors: Jbilou Khalide 
Published: December 2023

Abstract
In this work, we review different numerical methods for low order model reduction and linear control problems. These methods are essentially based on block, global, extended block or rational block Krylov subspace methods when we are dealing with large-scale dynamical systems. In this paper, the main schemes for model reduction are outlined, and personal contribution to some theoretical aspects is also given. Tools and approaches in model simplification, developed by some research groups are discussed, and the main results obtained during the last few years are summarized.
AMS subject classifications. 65F10, 65F30