bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Full Continuum of Robotic Breast Surgery: Robotic-assisted Mastectomy, Robotic DIEP Flap, and Robotic Supermicrosurgery
Authors: Tanna Neil , Sugiyama Gainosuke , Smith Mark L. , Sanchez Susana Benitez , Minasian Raquel A. , Robinson Emma , Silverman Julia , Shuck John W. , Selber Jesse 
Published: December 2023

Summary:
In recent years, robotic surgery has rapidly expanded to improve surgical outcomes in a variety of surgical subspecialties. Although plastic surgery has taken longer to integrate robotic surgery into practice, the advantages of robotic-assisted surgery, including improved visualization and resolution, minimally invasive approaches, and the ability to surpass human precision and scale, have driven its more recent adoption into plastic surgery. Currently, procedures performed with robotic assistance that are considered part of the continuum of surgical treatment of breast cancer include robotic-assisted nipple-sparing mastectomy, reconstruction with the robotic latissimus dorsi flap or the deep inferior epigastric artery perforator flap, and robotic microsurgery for the flap anastomosis and/or the surgical treatment of lymphedema. The authors provide an overview of robotic surgery and how it has been integrated into the field of plastic surgery, as well as a review of the most common procedures within the field where robotic assistance can be incorporated: nipple-sparing mastectomy, robotic latissimus flap, robotic deep inferior epigastric artery perforator flap breast reconstruction, and microvascular anastomoses.
Which lesions are not amenable to tricuspid clipping?
Authors: Bakar Shahrukh N. , Fam Neil P. 
Published: December 2023

Purpose of review
Tricuspid transcatheter edge-to-edge repair (T-TEER) has emerged as a well tolerated and effective therapeutic option for many patients with symptomatic severe tricuspid regurgitation at prohibitive surgical risk. However, there remain several important limitations to clip-based technology in the context of other rapidly emerging percutaneous treatment options for tricuspid regurgitation.

Recent findings
Tricuspid lesions pose unique challenges to treatment with the current toolbox of transcatheter clip-based technologies. This review will explore key issues related to patient factors, anatomical factors, and imaging factors that may render lesions to be unsuitable for treatment with T-TEER.

Summary
Selection for T-TEER must include a detailed clinical evaluation in the context of a ‘heart team’ approach involving multiple subspecialists, with screening for patient/lesion characteristics that make T-TEER suboptimal with current clip-based technologies. Future directions for research include patient-specific 3D modeling techniques, leaflet grasping techniques, clip deployment strategies, and personalized device sizing to increase the spectrum of lesions that may be treated with T-TEER within the context of other emerging transcatheter treatment options.
Biodosimetry Based on Gamma-H2AX Quantification in Human Peripheral Blood Lymphocytes after Partial-body Irradiation
Authors: Ma Li-Ping , Chen Jie , Liu Meng-Meng , Yan Juan , Xiang Jia-Qi , Tian Mei , Gao Ling , Liu Qing-Jie 
Published: December 2023

Abstract
Quantification of gamma-H2AX foci can estimate exposure to ionizing radiation. Most nuclear and radiation accidents are partial-body irradiation, and the doses estimated using the total-body irradiation dose estimation formula are often lower than the actual dose. To evaluate the dose-response relation of gamma-H2AX foci in human peripheral blood lymphocytes after partial-body irradiation and establish a simple and high throughput model to estimate partial-body irradiation dose, we collected human peripheral blood and irradiated with 0-, 0.5-, 1-, 2-, 3-, 4-, 5-, 6-, and 8-Gy gamma rays to simulate total-body irradiation in vitro. Gamma-H2AX foci were quantitated by flow cytometry at 1 h after irradiation, and a dose-response curve was established for total-body irradiation dose estimation. Then, a partial-body irradiation dose-response calibration curve was established by adding calibration coefficients based on the Dolphin method. To reflect the data distribution of all doses more realistically, the partial-body irradiation dose-response calibration curve was divided into two sections. In addition, partial-body irradiation was simulated in vitro, and the PBI data were substituted into curves to verify the accuracy of the two partial-body irradiation calibration curves. Results showed that the dose estimation variations were all less than 30% except the 25% partial-body irradiation group at 1 Gy, and the partial-body irradiation calibration dose-response curves were YF1 = − 3.444 x 2 + 18.532 x + 3.109, R2 = 0.92 (YF ≤ 27.95); YF2 = − 2.704 x 2 + 37.97 x − 56.45, R2 = 0.86 (YF > 27.95). Results also suggested that the partial-body irradiation dose-response calibration curve based on the gamma-H2AX foci quantification in human peripheral blood lymphocytes is a simple and high throughput model to assess partial-body irradiation dose.
An Alternative to Cytology in Triaging Cisgender Men and Transgender Women With HIV for High-Resolution Anoscopy
Authors: Mudrinich Shane M. , Nguyen Minh Ly T. , Blemur Danielle M. , Wang Xinzhu , Wang Yun F. , Krishnamurti Uma , Mosunjac Marina , Flowers Lisa C. 
Published: December 2023

Objectives
To evaluate high-risk human papillomavirus testing (hrHPV) as an alternative for anal cytology in screening for high-grade anal neoplasia (AIN2–3) among males with HIV. To identify predictive risk factors for AIN2–3 and develop a clinical tool to triage males with HIV for high-resolution anoscopy (HRA) without cytology.

Design
Retrospective cohort study of 199 adult cisgender men and transgender women with HIV referred to an anal neoplasia clinic in the Southeastern United States between January 2018 and March 2021.

Methods
Each subject underwent cytology, hrHPV, and HRA. Clinical and sociodemographic risk factors were collected for each subject. Significant risk factors for AIN2–3 were identified using logistic regression, and a triage tool incorporating these factors was developed. Screening test characteristics were calculated for cytology with and without adjunct hrHPV, hrHPV alone, and the triage tool.

Results
In multivariate analysis, significant predictors of AIN2–3 were hrHPV positivity (odds ratio [OR] = 11.98, CI = 5.58–25.69) and low CD4 count (OR = 2.70, CI = 1.20–6.11). There was no significant difference in positive or negative predictive values among the tool, stand-alone hrHPV, and anal cytology with adjunct hrHPV. Sensitivity and specificity were not significantly different for stand-alone or adjunctive hrHPV testing. Compared with cytology, stand-alone hrHPV and the novel triage tool reduced unnecessary HRA referrals by 65% and 30%, respectively.

Conclusions
Stand-alone hrHPV would have missed 11 of 74 AIN2–3 and generated 74 fewer unnecessary HRAs than current cytology-based screening patterns, which led to 115 unnecessary HRAs in our cohort. We propose triaging those with low CD4 count, hrHPV positivity, and/or smoking history for HRA.
2019 ASCCP Risk-Based Management Consensus Guidelines: Updates Through 2023
Authors: Perkins Rebecca B. , Guido Richard S. , Castle Philip E. , Chelmow David , Einstein Mark H. , Garcia Francisco , Huh Warner K. , Kim Jane J. , Moscicki Anna-Barbara , Nayar Ritu , Saraiya Mona , Sawaya George F. , Wentzensen Nicolas , Schiffman Mark ,   for the 2019 ASCCP Risk-Based Management Consensus Guidelines Committee
Published: December 2023

Abstract
This Research Letter summarizes all updates to the 2019 Guidelines through September 2023, including: endorsement of the 2021 Opportunistic Infections guidelines for HIV+ or immunosuppressed patients; clarification of use of human papillomavirus testing alone for patients undergoing observation for cervical intraepithelial neoplasia 2; revision of unsatisfactory cytology management; clarification that 2012 guidelines should be followed for patients aged 25 years and older screened with cytology only; management of patients for whom colposcopy was recommended but not completed; clarification that after treatment for cervical intraepithelial neoplasia 2+, 3 negative human papillomavirus tests or cotests at 6, 18, and 30 months are recommended before the patient can return to a 3-year testing interval; and clarification of postcolposcopy management of minimally abnormal results.
Factors Affecting Medical Residents’ Decisions to Work After Call
Authors: Carr Michele M. , Foreman Anne M. , Friedel Jonathan E. , O’Brien Daniel C. , Wirth Oliver 
Published: December 2023

Background
Accreditation Council for Graduate Medical Education (ACGME) work-hour restrictions (WHRs) are intended to improve patient safety by reducing resident fatigue. Compliance with ACGME WHRs is not universal.

Purpose
The purpose of this study was to identify factors that influence residents’ decisions to take a postcall day (PCD) off according to ACGME WHRs.

Methods
Residents (N = 433) at one university were emailed a link to a survey in 2019. The survey included demographic details and a Discrete Choice Experiment examining influences on resident decisions to take a PCD off.

Results
One hundred seventy-five residents (40.4%) responded to the survey; 113 residents (26%) completed the survey. Positive feedback from attending physicians about taking PCDs off in the past had the greatest impact on respondents’ decisions to take a PCD off, increasing the probability by 27.3%, followed by chief resident comments about the resident looking tired (16.6% increase), and having never heard their attendings comment about PCDs off as either positive or negative (13.9% increase). Factors that had the largest effect on decreasing the probability of taking a PCD were negative feedback about taking PCDs off (14.3% decrease), continuity of care concerns (10.8% decrease), and whether the resident was looking forward to an assignment (7.9% decrease).

Conclusions
The most important influencer of residents’ decisions to take a PCD off was related to feedback from their attending physicians, suggesting that compliance with WHRs can be improved by focusing on the residency program’s safety culture.
The Aesthetic Evaluation of the Brow-Eye Continuum After Correction of Severe Congenital Ptosis in Children With Extended Frontalis Muscle Advancement Technique
Authors: Sun Li-Ming , Fan Xiao , Hao Dong-Yue , Cao Jiao , Cang Zheng-Qiang , He Yun-Xia , Qiao Hai-Xia , Song Bao-Qiang , Peng Pai , Liu Chao-Hua 
Published: December 2023

Background
Severe congenital ptosis is a common ocular deformity in pediatric patients that can significantly impact visual development and aesthetic appearance, leading to negative psychosocial outcomes. The frontalis muscle advancement technique is a well-established surgical treatment for severe congenital ptosis. Aesthetic changes of the brow-eye continuum often plays an important role in ptosis surgery.

Methods
We conducted a single-center retrospective case series study of patients with severe congenital ptosis who underwent the frontalis muscle advancement technique at the Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University between April 2020 and June 2021. The study aimed to evaluate the aesthetic changes of the eyebrow-eyelid continuum after surgery. The main outcome measurements included marginal reflex distance 1, palpebral fissure height, eyebrow position, upper eyelid to lower eyebrow distance, lower eyelid to upper eyebrow distance, and nasal base to lower eyelid distance.

Results
The study included 48 patients (66 eyelids), with 30 unilateral and 18 bilateral patients. Our analysis found that eyebrow height decreased by an average of 4.8% postoperatively relative to preoperatively in all patients.

Conclusions
The frontalis muscle advancement technique has demonstrated effectiveness in achieving aesthetically pleasing outcomes in children with severe ptosis. It is crucial to pay careful attention to the brow-eye continuum during the correction process, as its harmony can greatly impact the final result.
Prospective Study of a System-Wide Adrenal Incidentaloma Quality Improvement Initiative
Authors: Woods Alison P , Feeney Timothy , Gupta Avneesh , Knapp Philip E , McAneny David , Drake Frederick Thurston 
Published: December 2023

BACKGROUND:
Appropriate follow-up of incidental adrenal masses (IAMs) is infrequent. We implemented a quality improvement (QI) program to improve management of IAMs.

STUDY DESIGN:
This system-wide initiative targeted primary care providers (PCPs) after IAM detection. It incorporated (1) chart-based messages and emails to PCPs, (2) an evidence-based IAM evaluation algorithm, (3) standardized recommendations in radiology reports, and (4) access to a multispecialty adrenal clinic. Patients diagnosed with an IAM from January 1, 2018, to December 31, 2019, were prospectively included (the “QI cohort”) and compared with a historical, preintervention cohort diagnosed with IAMs in 2016. The primary outcomes were the initiation of an IAM investigation by the PCP, defined as relevant clinical history-taking, laboratory screening, follow-up imaging, or specialist referral.

RESULTS:
The QI cohort included 437 patients and 210 in the historical cohort. All patients had 12 months or more of follow-up. In the QI cohort, 35.5% (155 of 437) met the primary endpoint for PCP-initiated evaluation, compared with 27.6% (58 of 210) in the historical cohort (p = 0.0496). Among the subgroup with a documented PCP working within our health system, 46.3% (74 of 160) met the primary endpoint in the QI cohort vs 33.3% (38 of 114) in the historical cohort (p = 0.035). After adjusting for insurance status, presence of current malignancy, initial imaging setting (outpatient, inpatient, or emergency department), and having an established PCP within our health system, patients in the QI cohort had 1.70 times higher odds (95% CI 1.16 to 2.50) of undergoing a PCP-initiated IAM evaluation. Adrenal surgery was ultimately performed in 2.1% (9 of 437) of QI cohort patients and 0.95% (2 of 210) of historical cohort patients (p = 0.517).

CONCLUSIONS:
This simple, moderately labor-intensive QI intervention was associated with increased IAM evaluation initiated by PCPs.
eRNA-IDO: a one-stop platform for identification, interactome discovery and functional annotation of enhancer RNAs
Authors: Zhang Yuwei , Gong Lihai , Ding Ruofan , Chen Wenyan , Rong Hao , Li Yanguo , Shameem Fawziya , Ali Korakkandan Arshad , Li Lei , Liao Qi 
Published: December 2023

AbstractIncreasing evidence proves the transcription of enhancer RNA (eRNA) and its important role in gene regulation. However, we are only at the infancy stage of understanding eRNA interactions with other biomolecules and the corresponding functionality. To accelerate eRNA mechanistic study, we present the first integrative computational platform for humaneRNAidentification, interactome discovery, and functional annotation, termed eRNA-IDO. eRNA-IDO comprises two modules: eRNA-ID and eRNA-Anno. Functionally, eRNA-ID identifies eRNAs fromde novoassembled transcriptomes. The bright spot of eRNA-ID is indeed the inclusion of 8 kinds of enhancer makers, whose combination enables users to personalize enhancer regions flexibly and conveniently. In addition, eRNA-Anno provides cell/tissue specific functional annotation for any novel and known eRNAs through discovering eRNA interactome from the prebuilt or user-defined eRNA-coding gene networks. The pre-built networks include GTEx-based normal co-expression networks, TCGA-based cancer co-expression networks, and omics-based eRNA-centric regulatory networks. Our eRNA-IDO carries sufficient practicability and significance for understanding the biogenesis and functions of eRNAs. The eRNA-IDO server is freely available athttp://bioinfo.szbl.ac.cn/eRNA_IDO/.
Continual improvement of multiplex mutagenesis in Arabidopsis
Authors: Develtere Ward , Decaestecker Ward , Rombaut Debbie , Anders Chantal , Clicque Elke , Vuylsteke Marnik , Jacobs Thomas B. 
Published: December 2023

ABSTRACTCRISPR/Cas9 is currently the most powerful tool to generate mutations in plant genomes and more efficient tools are needed as the scale of experiments increases. In the model plant Arabidopsis, the choice of promoter driving Cas9 expression is critical to generate germline mutations. Several optimal promoters have been reported. However, it is unclear which promoter is ideal as they have not been thoroughly tested side-by-side. Furthermore, most plant vectors still use one of the two Cas9 nuclear localization sequence (NLS) configurations initially reported and can still be optimized. We genotyped more than 6,000 Arabidopsis T2 plants to test seven promoters and eleven NLS architectures across 14 targets to systematically improve the generation of single and multiplex inheritable mutations. We find that the RPS5A promoter and double-BP NLS architecture were individually the most efficient components. When combined, 99% of T2 plant contained at least one knockout mutation and 84% contained 4-7-plex knock-outs. These optimizations will be useful to generate higher-order knockouts in the germline of Arabidopsis and likely be applicable to other CRISPR systems as well.
Comparison of mitochondrial response to SARS-CoV-2 spike protein receptor binding domain in human lung microvascular, coronary artery endothelial and bronchial epithelial cells
Authors: Kulkovienė Gabrielė , Narauskaitė Deimantė , Tunaitytė Agilė , Volkevičiūtė Augusta , Balion Zbigniev , Kutakh Olena , Gečys Dovydas , Kairytė Milda , Uldukytė Martyna , Stankevičius Edgaras , Jekabsone Aistė 
Published: December 2023

AbstractRecent evidence indicate that SARS-CoV-2 spike protein affects mitochondria with a cell type-dependent outcome. We elucidate the effect of SARS-CoV-2 receptor binding domain (RBD) on the mitochondrial network and cristae morphology, oxygen consumption, mitoROS production, and inflammatory cytokine expression in cultured human lung microvascular (HLMVEC) and coronary artery endothelial (HCAEC) and bronchial epithelial cells (HBEC). Live Mito Orange staining, STED microscopy and Fiji MiNa analysis were used for mitochondrial cristae and network morphometry, Agilent XFp analyser for mitochondrial/glycolytic activity, MitoSOX fluorescence for mitochondrial ROS, and qRT-PCR plus Luminex for cytokines. In HLMVEC, SARS-CoV-2 RBD fragmented the mitochondrial network, decreased cristae density, mitochondrial oxygen consumption and glycolysis and induced mitoROS-mediated GM-CSF and IL-1β expression in all three investigated cell types and IL-8 - in both endothelial cell types. Mitochondrial ROS control SARS-CoV-2 RBD-induced inflammation in HLMVEC, HCAEC and HBEC, with the mitochondria of HLMVEC being more sensitive to SARS-CoV-2 RBD.
Dynamic Multiplexed Control and Modeling of Optogenetic Systems Using the High-Throughput Optogenetic Platform, Lustro
Authors: Harmer Zachary P. , Thompson Jaron C. , Cole David L. , Zavala Victor M. , McClean Megan N. 
Published: December 2023

AbstractThe ability to control cellular processes using optogenetics is inducer-limited, with most optogenetic systems responding to blue light. To address this limitation we leverage an integrated framework combining Lustro, a powerful high-throughput optogenetics platform, and machine learning tools to enable multiplexed control over blue light-sensitive optogenetic systems. Specifically, we identify light induction conditions for sequential activation as well as preferential activation and switching between pairs of light-sensitive spit transcription factors in the budding yeast,Saccharomyces cerevisiae. We use the high-throughput data generated from Lustro to build a Bayesian optimization framework that incorporates data-driven learning, uncertainty quantification, and experimental design to enable the prediction of system behavior and the identification of optimal conditions for multiplexed control. This work lays the foundation for designing more advanced synthetic biological circuits incorporating optogenetics, where multiple circuit components can be controlled using designer light induction programs, with broad implications for biotechnology and bioengineering.Graphical abstract
Lactose blocks intercellular spreading of Galectin-1 from cancer cells to T-cells and activates tumor immunological control
Authors: Hong Yu , Si Xiaofang , Liu Wenjing , Mai Xueying , Zhang Yu 
Published: December 2023

AbstractUnderstanding the mechanisms by which the immune system surveils cancer is the key to developing better tumor immunotherapy strategies. By CRISPR/Cas9 screenings, we identified that inactivation of beta-1,4-galactosyltransferase-1 (B4GALT1), a key enzyme in glycoconjugate biosynthesis, leads to enhanced T-cell receptor (TCR) activation and functions of CD8+T-cells. Via proximity-dependent-intercellular-protein-spreading (PDICPS), cancer cells transfer surface-bound galectin-1 (Gal-1) proteins, which recognize and bind galactosylated membrane proteins, to CD8+T-cells, thereby suppressing T-cell-mediated cytolysis. B4GALT1-deficiency leads to reduced cell-surface galactosylation and Gal-1 binding of CD8+T-cells. Proteomic analysis revealed reduced binding of Gal-1 with TCR and its coreceptor CD8 on B4GALT1-deficient CD8+T-cells, leading to enhanced TCR-CD8 colocalization and T-cell activation. Lactose, a structure-mimicking competitive inhibitor of N-glycan galactosylation, enhances the functions of CD8+T-cells and tumor immunosurveillance. Results from various preclinical tumor models demonstrate that lactose and its derivatives are a new class of immune checkpoint inhibitors for tumor immunotherapy.
Brain tumor classification using ResNet50-convolutional block attention module
Authors: Oladimeji Oladosu Oyebisi , Ibitoye Ayodeji Olusegun J. 
Published: December 2023

PurposeDiagnosing brain tumors is a process that demands a significant amount of time and is heavily dependent on the proficiency and accumulated knowledge of radiologists. Over the traditional methods, deep learning approaches have gained popularity in automating the diagnosis of brain tumors, offering the potential for more accurate and efficient results. Notably, attention-based models have emerged as an advanced, dynamically refining and amplifying model feature to further elevate diagnostic capabilities. However, the specific impact of using channel, spatial or combined attention methods of the convolutional block attention module (CBAM) for brain tumor classification has not been fully investigated.Design/methodology/approachTo selectively emphasize relevant features while suppressing noise, ResNet50 coupled with the CBAM (ResNet50-CBAM) was used for the classification of brain tumors in this research.FindingsThe ResNet50-CBAM outperformed existing deep learning classification methods like convolutional neural network (CNN), ResNet-CBAM achieved a superior performance of 99.43%, 99.01%, 98.7% and 99.25% in accuracy, recall, precision and AUC, respectively, when compared to the existing classification methods using the same dataset.Practical implicationsSince ResNet-CBAM fusion can capture the spatial context while enhancing feature representation, it can be integrated into the brain classification software platforms for physicians toward enhanced clinical decision-making and improved brain tumor classification.Originality/valueThis research has not been published anywhere else.