bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical outcomes of compromised side branch (stent jail) after coronary stenting with the NIR stent
Authors: Bhargava Balram , Waksman Ron , Lansky Alexandra J. , Kornowski Ran , Mehran Roxana , Leon Martin B. 
Published: August 2002

AbstractAcute side‐branch (SB) compromise or occlusion stent jail after native coronary stenting is a matter of concern. Attempts at maintaining SB patency can be a technical challenge. The purpose of this study was to determine the clinical impact of SB compromise or occlusion in patients undergoing stenting of parent vessel lesions. We evaluated in‐hospital and long‐term clinical outcomes (death, Q‐wave myocardial infarction, and repeat revascularization rates at 6 months) in 318 consecutive patients undergoing NIR stent implantation across an SB. Based on independent angiographic analysis, 218 (68.6%) patients had no poststent SB compromise, 85 (26.7%) patients had narrowed SB (> 70% narrowing, without total occlusion), and 15 (4.7%) patients had an occluded SB after stent implantation. The baseline patient and lesion characteristics were similar between the groups. Procedural success was 100%. Patients with SB occlusion had a higher stents/lesion ratio (P < 0.006). Side‐branch occlusion was associated with higher in‐hospital ischemic complications (Q‐wave myocardial infarction, 7%; non–Q‐wave myocardial infarction, 20%; P < 0.05) compared to patients with SB compromise or normal SB. At 6‐month follow‐up, there was a trend for more myocardial infarctions in the group with SB occlusion during the index procedure (Q‐wave myocardial infarction, 7% vs. 1% in the narrowed and 0% in normal SB; P = 0.09). However, late target lesion revascularization and mortality were similar in the three groups (P = 0.91). SB occlusion after parent vessel stenting is associated with more frequent in‐hospital Q‐wave and non–Q‐wave myocardial infarctions. However, with the NIR stent, side‐branch compromise or occlusion does not influence late (6 month) major adverse events, including death, myocardial infarction, or need for repeat revascularization. Cathet Cardiovasc Intervent 2001;54:295–300. © 2001 Wiley‐Liss, Inc.
Transradial approach for renal artery stenting
Authors: Scheinert Dierk , Bräunlich Sven , Nonnast‐Daniel Barbara , Schroeder Malte , Schmidt Andrej , Biamino Giancarlo , Daniel Werner Günther , Ludwig Josef 
Published: August 2002

AbstractPercutaneous interventional procedures in the renal arteries are usually performed using a femoral or brachial vascular access. The transradial approach, which has been extensively investigated for coronary angiography and intervention, could be an attractive new technique for renal artery angioplasty and stenting. In 18 patients with hemodynamically relevant unilateral renal artery stenosis (mean diameter stenosis, 83% ± 18%; right, n = 7; left, n = 11), interventional treatment with PTA and stenting was performed using a left (n = 16) or right (n = 2) radial artery access. Indications for the transradial approach were acute aorto‐renal angles or severe peripheral arterial obstructions. After engagement of the renal artery ostium with a 6 Fr Multipurpose guiding catheter (length, 125 cm; Cordis) the stenosis was passed with a 0.014″ guidewire followed by stent implantation (ACS Multi‐Link RX Ultra, Guidant; length, 18 mm; diameter, 5 mm). Direct stenting was successfully performed in 16 cases. Predilatations were required in two cases. In five patients, optimal stent expansion was achieved by additional postdilatations. A primary technical success (residual stenosis < 30%) could be achieved in all cases. There were no periprocedural complications. According to color‐coded doppler ultrasound, all access site arteries showed a normal perfusion. Clinically blood pressure control was improved in 11 patients with a significant reduction in systolic and diastolic blood pressure values. Serum creatinine values dropped from 1.81 ± 0.3 mg/dl to 1.49 ± 0.3 mg/dl (P < 0.001). Transradial renal artery angioplasty and stenting is technically feasible and safe. Particularly in patients with unfavorable vessel anatomy, this new cranio‐caudal approach is an attractive alternative technique. Cathet Cardiovasc Intervent 2001;54:442–447. © 2001 Wiley‐Liss, Inc.
Comparison of three‐way resolution methods for non‐trilinear chemical data sets
Authors: de Juan Anna , Tauler Romà 
Published: August 2002

AbstractResolution of three‐way chemical data sets can be tackled using two families of chemometric methods: those assuming trilinear structure in the data set, such as direct trilinear decomposition (DTD) or parallel factor analysis (PARAFAC); and those which decompose the three‐way data set according to a model lacking this structure, such as TUCKER3 or multivariate curve resolution–alternating least squares (MCR–ALS). The first group of methods provides unique solutions, whereas the second group gives solutions subject to rotational ambiguities. DTD and PARAFAC are thus the choice to deal with chemical data sets with trilinear structure. However, in the analysis of chemical data with non‐trilinear structure, which is most commonly found in practice, the more ambiguous solutions given by TUCKER3 and MCR–ALS could be balanced by the major flexibility in the modelling of profiles. To assess this possibility, three‐way resolution methods from the two mentioned families are applied to simulated and real data sets designed to show typical non‐trilinear chemical situations, caused by shifts and shape changes in profiles. Copyright © 2001 John Wiley & Sons, Ltd.
A human homologue of Drosophila kelch associates with myosin‐VIIa in specialized adhesion junctions
Authors: Velichkova Michaella , Guttman Julian , Warren Carmen , Eng Lily , Kline Katie , Vogl A. Wayne , Hasson Tama 
Published: August 2002

AbstractMutations in myosin‐VIIa are responsible for the deaf‐blindness, Usher disease. Myosin‐VIIa is also highly expressed in testis, where it is associated with specialized adhesion plaques termed ectoplasmic specializations (ES) that form between Sertoli cells and germ cells. To identify new roles for myosin‐VIIa, we undertook a yeast two‐hybrid screen to identify proteins associated with myosin‐VIIa in the ES. We identified Keap1, a human homologue of the Drosophila ring canal protein, kelch. The kelch‐repeats in the C‐terminus of human Keap1 associate with the SH3 domain of myosin‐VIIa. Immunolocalization studies revealed that Keap1 is present with myosin‐VIIa in the actin bundles of the ES. Myosin‐VIIa and Keap1 copurify with ES and colocate with each other and with F‐actin at the electron microscopy level. Interestingly, in many epithelial cell types including cells derived from retina and inner ear, Keap1 is a component of focal adhesions and zipper junctions. Keap1 can target to the ES in the absence of myosin‐VIIa, suggesting that Keap1 associates with other molecules in the adhesion plaque. Keap1 and myosin‐VIIa overlapped in expression in the inner hair cells of the cochlea, suggesting that Keap1 may be a part of a family of actin‐binding proteins that could be important for myosin‐VIIa function in testis and inner ear. Cell Motil. Cytoskeleton 51:147–164, 2002. © 2002 Wiley‐Liss, Inc.
Villin‐type headpiece domains show a wide range of F‐actin‐binding affinities
Authors: Vardar D. , Chishti A. H. , Frank B. S. , Luna E. J. , Noegel A. A. , Oh S. W. , Schleicher M. , McKnight C. J. 
Published: August 2002

AbstractThe villin‐type “headpiece” domain is a modular motif found at the extreme C‐terminus of larger “core” domains in over 25 cytoskeletal proteins in plants and animals. Although headpiece is classified as an F‐actin‐binding domain, it has been suggested that some expressed fusion‐proteins containing headpiece may lack F‐actin‐binding in vivo. To determine the intrinsic F‐actin affinity of headpiece domains, we quantified the F‐actin affinity of seven headpiece domains and three N‐terminal truncations, under identical in vitro conditions. The constructs are folded and adopt the native headpiece structure. However, they show a wide range of affinities that can be grouped into high, low, and nonspecific‐binding categories. Computer models of the structure and charged surface potential of these headpiece domains suggest features important for high F‐actin affinity. We conclude that not all headpiece domains are intrinsically F‐actin‐binding motifs, and suggest that the surface charge distribution may be an important element for F‐actin recognition. Cell Motil. Cytoskeleton 52:9–21, 2002. © 2002 Wiley‐Liss, Inc.
Mutagenesis of β‐tubulin cysteine residues in Saccharomyces cerevisiae: Mutation of cysteine 354 results in cold‐stable microtubules
Authors: Gupta Mohan L. , Bode Claudia J. , Dougherty Cynthia A. , Marquez Rebecca T. , Himes Richard H. 
Published: August 2002

AbstractCysteine residues play important roles in the control of tubulin function. To determine which of the six cysteine residues in β‐tubulin are critical to tubulin function, we mutated the cysteines in Saccharomyces cerevisiae β‐tubulin individually to alanine and serine residues. Of the twelve mutations, only three produced significant effects: C12S, C354A, and C354S. The C12S mutation was lethal in the haploid, but the C12A mutation had no observable phenotype. Based on interactive views of the electron crystallographic structure of tubulin, we suggest that substitution of serine for cysteine at this position has a destabilizing effect on the interaction of tubulin with the exchangeable GTP. The two C354 mutations, although not lethal, produced dramatic effects on microtubules and cellular processes that require microtubules. The C354 mutant cells had decreased growth rates, a slowed mitosis, increased resistance to benomyl, and impaired nuclear migration and spindle assembly. The C354A mutation produced a more severe phenotype than the C354S mutation: the haploid cells had chromosome segregation defects, only 50% of cells in a culture were viable, and a significant percentage of the cells were misshapened. Cytoplasmic microtubules in the C354S and C354A cells were longer than in the control strain and spindle structures appeared shorter and thicker. Both cytoplasmic and spindle microtubules in the two C354 mutants were extremely stable to cold temperature. After 24 h at 4°C, the microtubules were still present and, in fact, very long and thick tubulin polymers had formed. Evidence exists to indicate that the C354 residue in mammalian tubulin is near the colchicine binding site and the electron crystal structure of tubulin places the residue at the interface between the α‐ and β‐subunits. The sulfhydryl group is situated in a polar environment, which may explain why the alanine mutation is more severe than the serine mutation. When the C12S and the two C354 mutations were made in a diploid strain, the mutated tubulin was incorporated into microtubules and the resulting heterozygotes had phenotypes that were intermediate between those of the mutated haploids and the wild‐type strains. The results suggest that the C12 and C354 residues play important roles in the structure and function of tubulin. Cell Motil. Cytoskeleton 49:67–77, 2001. © 2001 Wiley‐Liss, Inc.
Determining complicated winding patterns for shim coils using stream functions and the target‐field method
Authors: Brideson Michael A. , Forbes Larry K. , Crozier Stuart 
Published: August 2002

AbstractIn a magnetic resonance imaging equipment, gradient and shim coils are needed to produce a spatially varying magnetic field throughout the sample being imaged. Such coils consist of turns of wire wound on the surface of a cylindrical tube. Shim coils in particular, must sometimes be designed to produce complicated magnetic fields to correct for impurities. Streamline patterns for shim coils are much more complicated than those for gradient coils. In this work we present a detailed analysis of streamline methods and their application to shim coil design. A method is presented for determining the winding patterns to generate these complicated fields. © 2002 John Wiley & Sons, Inc. Concepts Magn Reson 14: 9–18, 2002
Aspects of equivalence as illustrated by phosphine complexes of transition metals
Authors: Nelson John H. 
Published: August 2002

AbstractThe various types of symmetry‐related nuclei present in transition metal complexes of phosphines are discussed. Differences between the solution and the solid states are considered. Examples of spectra are presented to illustrate the relationships between the types of equivalences and observed spectral line shapes. An introduction to solid‐state NMR spectroscopy is followed by descriptions of spectra that illustrate some of the types of information, which may be obtained from NMR spectroscopy of solid samples. © 2002 John Wiley & Sons, Inc. Concepts Magn Reson 14: 19–78, 2002
Expression of cyclin B1 in the metaplasia‐dysplasia‐carcinoma sequence of Barrett esophagus
Authors: Geddert Helene , Heep Hans J. , Gabbert Helmut E. , Sarbia Mario 
Published: August 2002

AbstractBACKGROUNDIt is known that proliferation is deregulated progressively during carcinogenesis in Barrett esophagus (BE). Cyclin B1 is a key protein for the regulation of G2‐M‐phase transition during the cell cycle and is essential for initiation of mitosis.METHODSUsing immunohistochemistry, samples of Barrett metaplastic specialized epithelium (SE; n = 36 samples), low‐grade dysplasia (LGD; n = 25 samples), high‐grade dysplasia (HGD; n = 25 samples), and invasive adenocarcinoma (CA; n = 46 samples) derived from 50 esophagectomy specimens were investigated for the expression of cyclin B1. The number of cyclin B1 positive cells was determined semiquantitatively. In addition, in SE, LGD, and HGD samples, the pattern of cyclin B1 expression was assessed by determination of the presence of positive cells in four mucosal compartments: the deep glandular zone, the lower crypt zone, the upper crypt zone, and the luminal surface.RESULTSCyclin B1 expression was found in all lesions under investigation. Regarding the percentage of positive cells, a marked increase of cyclin B1 positive cells was observed in SE samples compared with LGD samples and in HGD samples compared with CA samples (chi‐square test; P < 0.0001), nevertheless showing a broad overlap between the different lesions. Concerning staining patterns, in the majority of SE samples (72.2%), cyclin B1 positive cells were restricted to the glandular zone and the lower crypt zone. In contrast, an expansion of cyclin B1 positive cells to superficially located zones of the mucosa (the upper crypt zone and/or the luminal surface) was observed in the majority of LGD samples (96.0%) and HGD samples (100%; P < 0.0001).CONCLUSIONSOverexpression of cyclin B1 is a frequent and early finding in the metaplasia‐dysplasia‐carcinoma sequence in BE. It may contribute to the loss of growth control and, subsequently, to the development of tumors in this location. Cancer 2002;92:212–8. © 2002 American Cancer Society.
Increased expression of β‐catenin predicts better prognosis in nonsmall cell lung carcinomas
Authors: Hommura Fumihiro , Furuuchi Keiji , Yamazaki Koichi , Ogura Shigeaki , Kinoshita Ichiro , Shimizu Michio , Moriuchi Tetsuya , Katoh Hiroyuki , Nishimura Masaharu , Dosaka‐Akita Hirotoshi 
Published: August 2002

AbstractBACKGROUNDβ‐Catenin has been shown to function as a Wnt signaling molecule to stimulate cyclin D1 expression and cell growth in several kinds of tumors.METHODSThe authors immunohistochemically examined specimens of 217 surgically resected primary nonsmall cell lung carcinomas (NSCLCs) for β‐catenin expression and classified them semiquantitatively into three categories, including those with high, moderate, and low scores of expression.RESULTSHigh, moderate, and low scores of expression were found in 37 (17.1%), 145 (66.8%), and 35 (16.1%) tumors, respectively. β‐Catenin expression was not correlated with cyclin D1 expression, but was positively correlated with the Ki‐67 cell growth fraction (P = 0.04). The direct sequencing analysis for the β‐catenin gene mutation of 13 specimens of 217 tumors for the current study revealed no mutations. The relation between survival and β‐catenin expression was evaluated in 148 potentially curatively resected tumors with pathologic Stages I–IIIA. A trend toward better survival was found in patients with tumors having higher scores. In multivariate analysis, high β‐catenin expression was a significant and independent favorable prognostic factor (hazards ratio, 0.31; P = 0.007) as was pathologic stage. Analyzed by cell type, in nonsquamous cell carcinomas, patients with tumors having high scores survived a significantly longer time than those with tumors having moderate or low scores (5‐year survival rates, 84%, 55%, and 32%, respectively; P = 0.02), and high β‐catenin expression tended to be a favorable prognostic factor (hazards ratio, 0.32; P = 0.052).CONCLUSIONSThese results indicate that, in NSCLCs, increased expression of β‐catenin can predict favorable prognosis of patients with resected tumors, suggesting that accumulation of β‐catenin has no or little oncogenic effect via activation of the Wnt pathway, unlike in colon carcinomas or hepatomas. Cancer 2002;94:752–8. © 2002 American Cancer Society.DOI 10.1002/cncr.10213
Results of multimodality therapy for squamous cell carcinoma of maxillary sinus
Authors: Nibu Ken‐ichi , Sugasawa Masashi , Asai Masao , Ichimura Keiichi , Mochiki Masato , Terahara Atsuro , Kawahara Nobutaka , Asato Hirotaka 
Published: August 2002

AbstractBACKGROUNDA wide variety of modalities, including surgery, radiation therapy, and chemotherapy, alone or in combination, have been used for the treatment of squamous cell carcinoma (SCC) of the maxillary sinus to obtain better local control and maintain functions. However, there is still much controversy with regard to the optimum treatment.METHODSFrom 1987 to 1999, 33 patients with SCC of maxillary sinus were treated at the Department of Otolaryngology–Head and Neck Surgery, University of Tokyo Hospital. The treatment consisted of 30–40 grays (Gy) of preoperative radiotherapy with concomitant intraarterial infusion of 5‐fluorouracil and cisplatin followed by surgery and 30–40 Gy of postoperative radiotherapy, for tumors without skull base invasion. For tumors invading the skull base, preoperative systemic chemotherapy with or without radiotherapy was performed, instead of intraarterial chemotherapy, then followed by skull base surgery. The surgical procedures varied according to the extent of tumor. Results were compared with those of the 108 patients treated in our hospital from 1976 to 1982.RESULTSPartial maxillectomy was performed in 2 T2 patients and 12 T3 patients. Total maxillectomy was performed in 1 T2 patient, 3 T2 patients, and 7 T4 patients. Skull base surgery was performed in eight T4 patients. Orbital content and hard palate were preserved in 22 patients and 18 patients, respectively. The overall 5‐year survival rates were 86% in T 3 patients and 67 % in T4 patients, respectively.CONCLUSIONSOur multimodal treatment has provided favorable local control and survival outcome with good functional results. Cancer 2002;94:1476–82. © 2002 American Cancer Society.DOI 10.1002/cncr.10253
Serum folate and homocysteine levels in head and neck squamous cell carcinoma
Authors: Almadori Giovanni , Bussu Francesco , Galli Jacopo , Cadoni Gabriella , Zappacosta Bruno , Persichilli Silvia , Minucci Angelo , Giardina Bruno 
Published: August 2002

AbstractBACKGROUNDLocal and systemic metabolic alterations are always present in cancer. Carcinogenesis is associated with biochemical disorders, often nonspecific, that might promote or derive from tumoral progression. Thus, analysis of metabolic alterations may be a valuable approach to understanding the biochemistry of tumors and may provide a means of identifying new targets for therapy. The methionine cycle in particular has been extensively studied in human cancer.METHODSThe authors analyzed serum concentrations of two metabolites of such pathways, folate and homocysteine, in 42 patients affected by head and neck squamous cell carcinoma (HNSCC) in comparison with two control groups, composed of smokers and non smokers.RESULTSMean folate level was 5.8 ± 2.1 ng/mL in carcinoma patients, 9.1 ± 2.7 ng/mL in smoking controls, and 9.7 ± 2.2 ng/mL in non smoking controls, with a statistically significant difference between carcinoma patients and smokers (mean difference: −3.3ng/mL; 95% confidence interval [CI]: −4.234 to −2.366; P < 0.0001) and between carcinoma patients and non smokers (mean difference: −3.9ng/mL; 95% CI: −4.67 to −3.13; P < 0.0001). Mean total homocysteine level was 10.4 ± 5.3 μM in carcinoma patients, 7.8 ± 2.5 μM in the non‐smokers' group, and 8.3 ± 2.8 μM in the smokers' group, with statistically significant differences between carcinoma patients and smoking controls (mean difference: 2.1 μM; 95% CI: 0.7056 to 3.494; P = 0.0034) and between carcinoma patients and non smoking controls (mean difference: 2.6 μM; 95% CI: 1.381 to 3.819; P < 0.0001).CONCLUSIONSDifferences in serum levels of folate and homocysteine might arise from tumor development and consequent metabolic alterations or might precede and promote tumor progression. If hypofolatemia is a risk factor for head and neck carcinogenesis, it might suggest a role for folate as a novel chemopreventive agent both in patients with precancerous lesions and in patients with treated HNSCC at risk for loco‐regional recurrence and second primary tumors. Cancer 2002;94:1006–11. © 2002 American Cancer Society.DOI 10.1002/cncr.10343
Macrophage migration inhibitory factor evaluation compared with prostate specific antigen as a biomarker in patients with prostate carcinoma
Authors: Meyer‐Siegler Katherine L. , Bellino Michael A. , Tannenbaum Myron 
Published: August 2002

AbstractBACKGROUNDCytokines are polypeptides that constitute a class of chemical mediator molecules that modulate cell growth by inducing specific target gene expression. The objective of this study was to evaluate the clinical usefulness of serum evaluation of the cytokine macrophage migration inhibitory factor (MIF) in patients undergoing routine prostate specific antigen (PSA) screening.METHODSIn this preliminary, retrospective study, the authors report the development of an enzyme‐linked immunosorbent assay (ELISA) for MIF determination in serum samples. A polymerase chain reaction (PCR)‐based assay investigated associations between MIF expression and prostate carcinoma (CaP). The authors developed a relative quantitative reverse transcriptase‐PCR assay to determine MIF mRNA amounts within laser‐capture microscopy (LCM)‐dissected prostate epithelial cells.RESULTSA comparison of serum MIF levels and total PSA levels identified a positive correlation (correlation coefficient [r2] = 0.61; P < 0.001; n = 509 patients), suggesting an association between elevated serum concentrations of these proteins and CaP. A correlation of serum MIF levels with a diagnosis of CaP demonstrated that patients with a previous CaP diagnosis had significantly elevated serum MIF concentrations (mean ± standard deviation, 6.8 ± 0.87 ng/mL; P < 0.001). To associate altered serum MIF levels with MIF mRNA expression within prostate epithelial cells, LCM‐dissected prostate epithelial cells (formalin fixed biopsies from three different patients) were used to determine MIF mRNA amounts by PCR analysis. On average, MIF mRNA amounts were 6.5 times higher in CaP epithelial cells that were invasive to the margin compared with MIF mRNA amounts in normal prostate epithelial cells within the same biopsy specimen.CONCLUSIONSThe ELISA data from the current study suggested an association between increased MIF expression and CaP and suggested that serum MIF concentration may serve as a prognostic marker for CaP. Cancer 2002;94:1449–56. © 2002 American Cancer Society.DOI 10.1002/cncr.10354