bluuebunny/crossref_metadata_2025_split huggingface dataset:
Boosting production of cembratriene‐ol in Saccharomyces cerevisiae via systematic optimization
Authors: Yang Haiquan , Zhang Kunjie , Shen Wei , Xia Yuanyuan , Li Yiting , Chen Xianzhong 
Published: October 2023

AbstractCembratriene‐ol is a good biodegradable biopesticide ingredient with future potential applications in the field of sustainable agriculture. Cembratriene‐ol is a monocyclic diterpenoid compound that is synthesized only in the trichome gland of Nicotiana plants. In this study, geranylgeranyl diphosphate synthase gene ggpps from Taxus canadensis and cbts*Δp were heterologously expressed in Saccharomyces cerevisiae W303‐1A to successfully synthesize cembratriene‐ol. The titer of cembratriene‐ol was increased by 1.84‐fold compared to the control by overexpressing the S. cerevisiae bifunctional (2E,6E)‐farnesyl diphosphate synthase genes ERG20 and cbts*Δp under one promoter PGAP. The titer of cembratriene‐ol in the engineered S. cerevisiae BY4741 was increased by 1.39‐fold compared to the engineered S. cerevisiae W303‐1A. The titer of cembratriene‐ol in the engineered S. cerevisiae BY4741 was increased by 2.22‐fold compared to the control by overexpressing ERG20 and cbts*Δp, respectively, using two promoters PGAP. Cembratriene‐ol was found to be successfully synthesized via the integrated expression of cbts*Δp, ggpps and ERG20 on the genome of S. cerevisiae BY4741. The titer of cembratriene‐ol in S. cerevisiae S25 was further increased by 1.80‐fold compared to the control via dynamic control of the squalene synthase gene ERG9. Overexpression of the genes cbts*Δp and ggpps using pY26‐GPD‐TEF in S. cerevisiae S25 with their integration expression increased the titer of cembratriene‐ol by 26.1‐fold compared to S. cerevisiae S25. The titer of cembratriene‐ol was significantly enhanced by mitochondrial compartmentalized expression of cbts*Δp and ggpps, which was 76.3‐fold higher than that of the initial strain constructed. It was indicated that the systematic optimization has great potential in facilitating high‐level production of cembratriene‐ol production in S. cerevisiae.
President's page
Authors: Hirsh Sandy 
Published: October 2023

EDITOR'S SUMMARYThe ASIS&T strategic plan has been the predominant concern of ASIS&T board members leading into, during and following their retreat in July 2015. After updates from committees, chapters, SIGs and others, the Board, along with past and future leadership, focused on the findings of the Strategic Planning Task Force (SPTF). These included analysis of member input and reports from internal and external sources, together with a resulting compilation of proposed strategic directions. The directions identified generally address membership, programs, communications, outreach and institutional knowledge. The Board brainstormed goals and objectives for each of the five strategic directions. Individual board members will join working groups representing each of the directions to refine the goals and objectives. A draft strategic plan will be posted online by October 2015 to stimulate and solicit member feedback. The draft will be revised and presented to the Board for adoption at the 2015 Annual Meeting, and subsequently reviewed and revised as appropriate. Thanks are extended to the many members and leaders who participated in the strategic planning process.
Copyright Considerations for Providing 3D Printing Services in the Library
Authors: Chan Jessica R. M. , Enimil Sandra Aya 
Published: October 2023

EDITOR'S SUMMARY3D printing enables physical objects to be constructed from a virtual 3D model with the aid of a computer‐aided design (CAD) program. The CAD files and printed physical product may be protected by copyright law, covering rights to reproduce and distribute copies of the work, make derivative works and publicly display or perform the work. Copyright does not cover useful articles or works in the public domain. Libraries could become entangled in copyright infringement directly or secondarily by providing the equipment that may be used to infringe on a copyright. Libraries can manage their risks by developing and implementing policies regarding 3D printer use, including using a mediated service model and being mindful of the “unsupervised copying” exception in the copyright law for libraries and archives. Patron education on 3D printing provides an opportunity to explain library policies on use and copyright issues.
Data‐Driven Society Begins with Data‐Savvy Youth
Authors: Ercegovac Zorana 
Published: October 2023

EDITOR'S SUMMARYWith data literacy an increasingly critical skill, dedicated instruction should start early in the educational process. By secondary school students should understand the distinction between data and information and the scope of the concept, and they should think critically about authorship, quality, source, collection, access, sharing and reuse. Instruction in data literacy requires access to large repositories of digital data, enabling students to develop skills in data description and tagging, prompting critical thinking about trustworthiness, reliability and validity, as well as audiences, temporal attributes, representations and evaluative sources. Data can be the focal point of a variety of classroom projects and integrated across disciplines, and there are many sources of data that can be readily accessed for classroom projects.
Survival outcomes and surgical morbidity based on surgical approach to pulmonary metastasectomy in pediatric, adolescent and young adult patients with osteosarcoma
Authors: Kuo Christopher , Malvar Jemily , Chi Yueh‐Yun , Kim Eugene S. , Shah Rachana , Navid Fariba , Stein James E. , Mascarenhas Leo 
Published: October 2023

AbstractBackgroundThoracotomy is considered the standard surgical approach for the management of pulmonary metastases in osteosarcoma (OST). Several studies have identified the advantages of a thoracoscopic approach, however, the clinical significance of thoracotomy compared to thoracoscopy is yet to be evaluated in a randomized trial.AimsThe primary aim was to determine the survival outcomes in OST patients based on surgical approach for pulmonary metastasectomy (PM) and secondary aim was to assess the post‐operative morbidities of OST PM through various surgical approaches.Materials and MethodsWe conducted a single institution retrospective study to compare survival outcomes and surgical morbidity according to the surgical approach of the management of pulmonary metastases in patients with OST.ResultsSixty‐one patients with OST underwent PM. Twenty‐one patients were metastatic at diagnosis and underwent PM during primary treatment; nine had thoracotomy, six thoracoscopy, and six combined thoracoscopy with thoracotomy (CTT). Forty‐three patients with first pulmonary relapse or progression underwent PM; 18 had thoracotomy, 16 thoracoscopy and nine CTT. There was no difference in survival between surgical approaches. There were significantly more postoperative morbidities associated with thoracotomy for initial PM (pain and postoperative chest tube placement), and for PM at first relapse (pneumothoraces, pain, Foley catheter use and prolonged hospitalizations).ConclusionOur study demonstrates that patients with OST pulmonary metastases have comparable poor outcomes despite varying surgical approaches for PM. There were significantly more postoperative morbidities associated with thoracotomy for PM. Surgical bias and other competing risks could not be assessed given the limitations of a retrospective study and may be addressed in a prospective trial evaluating surgical approach for PM in OST.
Analysing Megasynthetase Mutants at High Throughput Using Droplet Microfluidics**
Authors: Pourmasoumi Farzaneh , Hengoju Sundar , Beck Katharina , Stephan Philipp , Klopfleisch Lukas , Hoernke Maria , Rosenbaum Miriam A. , Kries Hajo 
Published: October 2023

AbstractNonribosomal peptide synthetases (NRPSs) are giant enzymatic assembly lines that deliver many pharmaceutically valuable natural products, including antibiotics. As the search for new antibiotics motivates attempts to redesign nonribosomal metabolic pathways, more robust and rapid sorting and screening platforms are needed. Here, we establish a microfluidic platform that reliably detects production of the model nonribosomal peptide gramicidin S. The detection is based on calcein‐filled sensor liposomes yielding increased fluorescence upon permeabilization. From a library of NRPS mutants, the sorting platform enriches the gramicidin S producer 14.5‐fold, decreases internal stop codons 250‐fold, and generates enrichment factors correlating with enzyme activity. Screening for NRPS activity with a reliable non‐binary sensor will enable more sophisticated structure‐activity studies and new engineering applications in the future.
Epstein–Barr virus infection leading to multiorgan involvement in an immunocompetent man
Authors: Paymannejad Saina , Shirani Kiana , Najafi Mohammad Amin , Khorvash Farzin , Shamlou Farid 
Published: October 2023

Key Clinical MessageEpstein–Barr virus (EBV) is a commonly asymptomatic widespread human herpes virus affecting over 90% of the population. It mostly originates complications like simple sore throat and infectious mononucleosis but severe manifestations are rare. Herein we report a 30‐year‐old immunocompetent man who presented with fever, sore throat, general weakness, and drowsiness. The diagnosis was formulated based on the positive RT‐PCR test for EBV DNA and serological detection of IgM antibody against viral capsid antigen. The patient developed severe meningoencephalitis, myocarditis, and bowel perforation and passed away after 72 days of hospitalization.
Development of Fine Particle Mechanical Separation Processes with Representative Catalyst Materials for Recycling PEM Water Electrolyzers Exploiting their Wetting Characteristics
Authors: Ahn Sohyun , Rudolph Martin 
Published: October 2023

AbstractDemand for technologies using water electrolysis to produce green hydrogen is increasing, although recycling research on membrane electrode assemblies, which contain various precious and highly critical metals, is still limited. This study therefore aims at exploiting the feasibility of fine particle separation processes based on the difference in hydrophobicity of the ultrafine materials used as catalysts in polymer electrolyte membrane electrolyzers and at providing a fundamental study with representative materials of carbon black and TiO2. Since the cathode materials including carbon black are hydrophobic and the anode materials as well as TiO2 are hydrophilic, the characterizations of their various surface properties such as zeta potentials, dispersion characteristics, and bubble coverage angle tests have been investigated. In addition, using liquid‐liquid particle extraction in a mixture model, 99 % of carbon black is recovered in the organic phase and 97 % of TiO2 is selectively separated in the aqueous phase with the help of the dispersant, sodium hexametaphosphate.
Catalyst‐Free Propargylboration of Ketones with Allenyl‐Bpins: Highly Stereoselective Synthesis of tert‐Homopropargyl Alcohols Bearing Vicinal Stereocenters
Authors: Zhang Qian‐Cheng , Zhong Qin , Zhao Jian 
Published: October 2023

AbstractA practical and efficient propargylboration of ketones is presented using general allenylboronic acid pinacol esters (allenyl‐Bpins) without a catalyst. This reaction is triggered by in‐situ activation of stable allenyl‐Bpins through the sequential addition of 1.25 equiv. of nBuLi and the prerequisite 2.0 equiv. of TFAA. Under the optimized reaction conditions, the versatile trisubstituted allenyl‐Bpins react with various ketones smoothly to afford a wide range of tert‐homopropargyl alcohols bearing vicinal stereocenters in high yields with good to excellent diastereoselectivities. Furthermore, propargylboration of ketones with chiral trisubstituted allenyl‐Bpins allows for the asymmetric synthesis of chiral tert‐homopropargyl alcohols with a full chirality transfer.
Automatisierte Demontage elektrischer Antriebsaggregate: Herausforderungen und Lösungsansätze
Authors: Hansen Florian , Hochstädt Daniel , Sauter Andreas , Seelig Jan H. 
Published: October 2023

AbstractDie automatisierte Demontage von Antriebsaggregaten aus Elektrofahrzeugen ist eine Möglichkeit, die Elektromobilität der Zukunft nachhaltiger zu gestalten. Zunächst werden die Hintergründe und der Stand der Technik beleuchtet sowie die Vorteile einer automatisierten Demontage aufgezeigt. Hieraus ergeben sich konkrete Herausforderungen, von denen einige aufgegriffen und Lösungsansätze präsentiert werden. Diese umfassen die Konzeption des Demontagemoduls, das Handling des Modellprodukts und Lösungen zur automatisierten zerstörungsfreien Demontage des Rotors sowie von Schraubverbindungen.
70 Jahre Verfahrenstechnik an der TU Dresden
Authors: Beckmann Michael , Babick Frank , Eckert Kerstin , Heitkam Sascha , Schubert Markus , Urbas Leon , Stintz Michael 
Published: October 2023

AbstractAn der Technischen Hochschule Dresden, der Vorgängereinrichtung der TU Dresden, wurde 1953 mit der Besetzung der Fachprofessur für Verfahrenstechnik eine Fachrichtung „Ingenieur für Verfahrenstechnik“ eingerichtet und das heutige Institut für Verfahrenstechnik und Umwelttechnik begründet. Seitdem wurde das Profil in Ausbildung und Forschung des Instituts innerhalb der Fakultät Maschinenwesen der Technische Universität Dresden (TUD) erheblich weiterentwickelt. Ausgehend von dieser Entwicklung werden die heutigen Forschungsschwerpunkte der fünf Professuren und Arbeitsgruppen vorgestellt.
Folic Acid‐Modified Nanoprobe for In Vivo‐Targeted Persistent Luminescence Imaging and pH‐Responsive Antibiotic Therapy of Bacterial Infection
Authors: Wang Li‐Ya , Yan Li‐Xia , Zhao Xu , Chen Li‐Jian , Yan Xiu‐Ping 
Published: October 2023

AbstractLow antibiotic utilization and inability to achieve real‐time monitoring of pathological status and treatment processes often result in unsatisfactory performance against bacterial infection. Developing a targeting antibacterial nanoprobe combining imaging with stimulus‐response antibiotic release is a promising strategy to precisely recognize lesions and enhance therapeutic efficacy for bacterial infection. In this work, we report a pH‐responsive theragnostic nanoplatform for targeted imaging and local drug release at the bacterial infection site. The nanoplatform consists of the core‐shell structure with persistent luminescence nanoparticles (PLNPs) as the core for autofluorescence‐free luminescence imaging and zeolitic imidazolate framework‐8 (ZIF‐8) as the shell to act as a carrier for antibiotics cefazolin. The core‐shell nanostructure is further conjugated with folic acid to facilitate the uptake and accumulation of the nanoparticles, and realize the autofluorescence‐free targeted imaging of the infection site. The acidic microenvironment at the bacterial infection site enables ZIF‐8 to decompose for specific drug release improve the performance in bacterial infection treatment. The developed pH‐responsive nanotheranostic probe is promising for autofluorescence‐free targeted imaging and therapy of bacterial infection.
Analysis of timing jitter in digital transmission systems
Authors: Miyauchi Kazuhiro , Wakabayashi Isamu , Shibayama Hiroki 
Published: October 2023

AbstractAn analysis of timing jitter generation in band‐limited digital systems is presented. An integral representation of jitter spectral density in frequency domain is derived for typical transmission systems: baseband polar system, BPSK and QPSK with AWGN. Using the representation, one can calculate the jitter spectral density and jitter variance for any frequency characteristics of the channel by performing integration over a finite frequency domain. The jitter spectral density consists of three terms with different noise dependency, each of which is represented by a combination of three factors: a coefficient, tank function, and jitter source spectral density. As a typical application of the present theory, the jitter spectral density and rms jitter are calculated for a cosine rolloff scheme. The jitter dependency on the rolloff factor, Eb/N0, and timing tank parameters are calculated and discussed. © 2001 Scripta Technica, Electron Comm Jpn Pt 1, 84(8): 1–13, 2001
Impact of adherence to guideline‐directed therapy on risk of death in HF patients across an ejection fraction spectrum
Authors: Chen Xiaojing , Kang Yu , Dahlström Ulf , Fu Michael 
Published: October 2023

AbstractAimsHow different degrees of adherence to guideline‐directed medical therapy (GDMT) affect mortality risk in patients with heart failure (HF) in a real‐world clinical setting is poorly understood. This study sought to investigate how different levels of adherence to GDMT were associated with the risk of all‐cause mortality in patients with HF across a spectrum of left ventricular ejection fractions (LVEFs) in a real‐world clinical setting.Methods and resultsA total of 64 610 HF patients with no missing value of LVEF from the Swedish Heart Failure Registry were included in the study. Patients were divided according to different LVEFs (<30%, 30–39%, 40–49%, and≥50%) and stratified by an adherence score (good, moderate, or poor) according to the triple, double, and single one usage of GDMT: angiotensin‐converting enzyme inhibitors/angiotensin receptor blockers, beta‐blockers, and mineralocorticoid receptor antagonists. The outcome is time to all‐cause mortality. The mean age of the whole cohort was 73.9 ± 12.1 years, and the proportion of patients in LVEF < 30%, 30–39%, 40–49%, and≥50% groups was 27.6%, 26.9%, 22.1%, and 23.3%, respectively. Patients with LVEF < 30% had the highest mortality rate, almost 20% higher than those with LVEF ≥ 50% {hazard ratio [HR] [95% confidence interval (CI)]: 0.80 [0.71–0.90], P < 0.001}. After treatment of GDMT with good adherence, patients with LVEF < 30% had similar mortality to those with LVEF ≥ 50% [HR (95% CI): 0.97 (0.86–1.10), P = 0.664]. However, the percentage of moderate or poor GDMT was alarmingly high, with good adherence only in 20% of the patients.ConclusionsGood adherence to GDMT works best in patients with LVEF < 50%, whereas moderate adherence to GDMT varies in efficacy depending on the components of the drug combinations.
Machine learning‐based classification of Alzheimer's disease and its at‐risk states using personality traits, anxiety, and depression
Authors: Waschkies Konrad F. , Soch Joram , Darna Margarita , Richter Anni , Altenstein Slawek , Beyle Aline , Brosseron Frederic , Buchholz Friederike , Butryn Michaela , Dobisch Laura , Ewers Michael , Fliessbach Klaus , Gabelin Tatjana , Glanz Wenzel , Goerss Doreen , Gref Daria , Janowitz Daniel , Kilimann Ingo , Lohse Andrea , Munk Matthias H. , Rauchmann Boris‐Stephan , Rostamzadeh Ayda , Roy Nina , Spruth Eike Jakob , Dechent Peter , Heneka Michael T. , Hetzer Stefan , Ramirez Alfredo , Scheffler Klaus , Buerger Katharina , Laske Christoph , Perneczky Robert , Peters Oliver , Priller Josef , Schneider Anja , Spottke Annika , Teipel Stefan , Düzel Emrah , Jessen Frank , Wiltfang Jens , Schott Björn H. , Kizilirmak Jasmin M. 
Published: October 2023

AbstractBackgroundAlzheimer's disease (AD) is often preceded by stages of cognitive impairment, namely subjective cognitive decline (SCD) and mild cognitive impairment (MCI). While cerebrospinal fluid (CSF) biomarkers are established predictors of AD, other non‐invasive candidate predictors include personality traits, anxiety, and depression, among others. These predictors offer non‐invasive assessment and exhibit changes during AD development and preclinical stages.MethodsIn a cross‐sectional design, we comparatively evaluated the predictive value of personality traits (Big Five), geriatric anxiety and depression scores, resting‐state functional magnetic resonance imaging activity of the default mode network, apoliprotein E (ApoE) genotype, and CSF biomarkers (tTau, pTau181, Aβ42/40 ratio) in a multi‐class support vector machine classification. Participants included 189 healthy controls (HC), 338 individuals with SCD, 132 with amnestic MCI, and 74 with mild AD from the multicenter DZNE‐Longitudinal Cognitive Impairment and Dementia Study (DELCODE).ResultsMean predictive accuracy across all participant groups was highest when utilizing a combination of personality, depression, and anxiety scores. HC were best predicted by a feature set comprised of depression and anxiety scores and participants with AD were best predicted by a feature set containing CSF biomarkers. Classification of participants with SCD or aMCI was near chance level for all assessed feature sets.ConclusionOur results demonstrate predictive value of personality trait and state scores for AD. Importantly, CSF biomarkers, personality, depression, anxiety, and ApoE genotype show complementary value for classification of AD and its at‐risk stages.
Perioperative complications among head and neck surgery patients with COVID‐19
Authors: Martin Ann M. , Elliott Zachary T. , Chisolm Paul , Crossley Jason , Maxwell Jessica H. , Pierce Matthew , Giurintano Jonathan 
Published: October 2023

AbstractBackgroundPatients undergoing surgery for head and neck cancer (HNC) have potentially high perioperative complication rates. Recent studies indicate that preoperative COVID‐19 infection poses increased risk for postoperative complications in other fields. However, to date, there has not been data showing the effect of COVID‐19 on complication rates for HNC. Here, a large database was employed to assess if perioperative COVID‐19 increased the risk of perioperative complications among those undergoing HNC surgery.MethodsA retrospective investigation was conducted using a multi‐institutional research database. Subjects who underwent HNC surgery from January 2020 to September 2022 were identified using the International Classification of Diseases and Current Procedure Terminology codes. Thirty‐day surgical and medical complications were assessed for those diagnosed with COVID‐19 infection from 7 days before or after surgery compared to those who were COVID‐19 negative. Cohorts were propensity scores matched by age, sex, and race.ResultsPerioperative COVID‐19 was present in n = 208 and absent in n = 15 158 subjects that underwent HNC surgery. For unmatched analyses, there was a statistically significant increased risk in the 30‐day postoperative period in COVID‐19‐positive patients for the following surgical complications: surgical site fistula, free tissue transfer (FTT) complication, FTT failure, and death. Additionally, there was a statistically significant increased risk in the 30‐day postoperative period in COVID‐19‐positive patients for the following medical complications: ventilator support, pneumonia, vasopressor, acute renal failure, and myocardial infarction.ConclusionThis large, retrospective populational study suggests HNC patients are at increased risk for death and several perioperative complications. This investigation is the first to address this clinical question.
The evolutionary advantages of chelonians are making them vulnerable to extinction in the Anthropocene
Authors: Bu Rongping , Lee Tien M. , Shi Haitao 
Published: October 2023

AbstractChelonians have survived for more than 200 million years. The distinct form of the chelonian shell stands as a testament to their enduring success, having survived millions of years of natural selection pressures. Unfortunately, traits and strategies that once ensured their survival—including their shell, strong environmental tolerance, long lifespan, and temperature‐dependent sex determination—are turning into disadvantages, particularly in the Anthropocene. This article outlines how this evolutionary success story is now on the brink of an extinction crisis.