bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analisis Pengetahuan Investasi, Return, dan Risiko Terhadap Minat Berinvestasi Online di Aplikasi Bibit
Authors: Amanda Keisya Tri , Tanjung Ahmad Albar 
Published: October 2023

This study will evaluate and assess investment knowledge, as well as returns and risks to an interest in investing online in BIBIT applications. The goal of this research is to measure and analyze investment knowledge. The amount of interest one has in making investments is serving as the dependent variable for this investigation. Because of the more rigorous and planned approach that this research method takes to the collection and analysis of numerical data, it can be classified as a type of quantitative research. For both the collecting and the analysis of data, this study relied on primary sources. Whereas the primary data is made up of information that was acquired through the direct and online distribution of questionnaires using a Google form, the secondary data is made up of information that was gathered through other means. The survey was completed by one hundred individuals, and the researchers collected a total of fifty samples from those individuals. The sample determination technique used a purposive sampling technique. The young people who take part in seed applications are the ones who are targeted and targeted for this activity. According to the data, having knowledge in investing led to an increase in the level of investment interest displayed in BIBIT applications. Increased investing activity is typically encouraged when a higher rate of return is offered. In a similar vein, there is a connection between the risk of an investment and the interest in that investment.
COMPARISON OF ANTI-DIARRHEAL ACTIVITY OF METHANOL AND ETHANOL EXTRACTSS OF WATERMELON RIND(CITRULLUS LANATUS L.) IN WHITE MICE (MUS MUSCULUS)
Authors: Arifianto Nasruhan , Munawaroh  , Ermawati  , Wahyuni  
Published: October 2023

Watermelon (Citrulluslanatus L.) is one of the most abundant fruit-producing plants in Indonesia. Watermelon is generally only consumed on the red flesh, while the rind is less desirable so that it becomes waste. The purpose of this study was to comparison of anti-diarrheal activity of methanol and 70% ethanol extractss of watermelon rind (citrulluslanatus l.) in white miice (musmuscucolus. This study used a protection method with the induction of MgSO4 (Magnesium Sulfate) by calculating the frequency of diarrhea, changes in stool consistency and stool weight. The data obtained from the activity test results of the watermelon rind methanol extracts against white mice were statistically analyzed using the one-way ANOVA test.Methanolic and 70% ethanolicextractss of watermelon rind (Citruluslanatus L.) contain tannin compounds that have antidiarrheal activity against mice (Musmusculus). From the results of the one-way ANOVA test, the weight of feces, the frequency of diarrhea and the consistency of the stools in the methanol extracts of watermelon rind at a dose of 100 mg/KgBW gave an optimal effect, because the weight of the feces obtained a value of p = 0.633, the frequency of diarrhea obtained a value of p = 0.899 and the consistency of the fecesobtained a value of p = 0.899 p-value = 0.525 compared to the positive control (loperamide). Ethanol extracts of Citrulluslanatus L. had antidiarrheal effect at a dose of 100 mg/kgBW, 200 mg/kgBW, and 300 mg/kgBW. Based on the results of the One Way ANOVA statistical test, the ethanolicextracts of Citrulluslanatus L. had an antidiarrheal effect at a dose of 100 mg/kgBW and the best dose was a dose of 300 mg/kgBW which was significant with the positive control group loperamide P value = 0.000, meaning that the ethanolicextracts of Citrulluslanatus L. at a dose of 300 mg/kgBW had almost the same effect as the loperamide positive control group based on the parameters of stool weight, frequency of diarrhea, and stool consistency. From this data 70% Ethanolic extracts better than methanolicextracts for antidiarrheal activity in mice.
ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI KEPUASAN KONSUMEN PRODUK SUSU MURNI KPUB SAPI JAYA MENGGUNAKAN METODE REGRESI LINEAR BERGANDA
Authors: Aziz Muslih  , Sumarsono  , Andhika Mayasari  
Published: October 2023

Customer satisfaction became one major priority for business agent to attract customer buying interest. Customer satisfaction is the feeling of happiness or sadness which appear after buying and comparing the product with other brands. The process of getting good Multiple Linear Regression analysis result in this research, then it has to be examined in these following steps: (1) Testing the validity and reliability of questionnaire measuring instruments. (2) Describing variables of the research. (3) Testing the classical assumptions such as heterokedasticity, multicollinearity, collinearity and normality test. (4) Analyzing Multiple Linear Regression, which includes the F_test (joint test), t_test (partial test), and the coefiicient of detemination (R-squared) test. Identification values of the factors that affect customer satisfaction towards KPUB Sapi Jaya pure milk products obtained from the result of the linear regression equation, where the value of the influence constituent is 2,117 units, the value of the influence of X1. of 0.367 units, the value of influence X3. Product features of 0.671 units, the value of influence X4. The product beauty is 0.259, the value of influence X5. The perceived quality is 0.814 units and the value of influence X6. Service is 0.211 units. Furthermore, the results of the significance test, which significantly affects customer satisfaction of KPUB Sapi Jaya pure milk products, it is concluded that there are 2 factors, the first is product durability factor (X1) and the second is perceived quality factor (X5). Meanwhile 4 other factors are not significant, such as product suitability factor (X2); product features (X3); beauty products (X4) and services (X6).
The prevalence of thyroid dysfunction and hyperprolactinemia in women with PCOS
Authors: van der Ham Kim , Stekelenburg Karlijn J. , Louwers Yvonne V. , van Dorp Wendy , Schreurs Marco W. J. , van der Wal Ronald , Steegers-Theunissen Régine P. M. , Laven Joop S. E. 
Published: October 2023

IntroductionOvulatory dysfunction is usually caused by an endocrine disorder, of which polycystic ovary syndrome (PCOS) is the most common cause. PCOS is usually associated with estrogen levels within the normal range and can be characterized by oligo-/anovulation resulting in decreased progesterone levels. It is suggested that decreased progesterone levels may lead to more autoimmune diseases in women with PCOS. In addition, it is often claimed that there is an association between hyperprolactinemia and PCOS. In this large well-phenotyped cohort of women with PCOS, we have studied the prevalence of thyroid dysfunction and hyperprolactinemia compared to controls, and compared this between the four PCOS phenotypes.MethodsThis retrospective cross-sectional study contains data of 1429 women with PCOS and 299 women without PCOS. Main outcome measures included thyroid stimulating hormone (TSH), Free Thyroxine (FT4), and anti-thyroid peroxidase antibodies (TPOab) levels in serum, the prevalence of thyroid diseases and hyperprolactinemia.ResultsThe prevalence of thyroid disease in PCOS women was similar to that of controls (1.9% versus 2.7%; P = 0.39 for hypothyroidism and 0.5% versus 0%; P = 0.99 for hyperthyroidism). TSH levels were also similar (1.55 mIU/L versus 1.48 mIU/L; P = 0.54). FT4 levels were slightly elevated in the PCOS group, although within the normal range (18.1 pmol/L versus 17.7 pmol/L; P &lt; 0.05). The prevalence of positive TPOab was similar in both groups (5.7% versus 8.7%; P = 0.12). The prevalence of hyperprolactinemia was similarly not increased in women with PCOS (1.3%% versus 3%; P = 0.05). In a subanalysis of 235 women with PCOS and 235 age- and BMI-matched controls, we found no differences in thyroid dysfunction or hyperprolactinemia. In according to differences between PCOS phenotypes, only the prevalence of subclinical hypothyroidism was significantly higher in phenotype B (6.3%, n = 6) compared to the other phenotypes.ConclusionWomen with PCOS do not suffer from thyroid dysfunction more often than controls. Also, the prevalence of positive TPOab, being a marker for future risk of thyroid pathology, was similar in both groups. Furthermore, the prevalence of hyperprolactinemia was similar in women with PCOS compared to controls.
Metabolic dysfunction-associated fatty liver disease is an early predictor for testosterone deficiency in aging men without metabolic syndrome
Authors: Liu Chia-Chu , Huang Shu-Pin , Lee Yung-Chin , Lee Cheng-Hsueh , Huang Tsung-Yi , Geng Jiun-Hung , Chang Che-Wei , Lin Chung-Yu , Juan Yung-Shun , Wu Wen-Jeng , Hsieh Tusty-Jiuan 
Published: October 2023

Background and aimsMetabolic dysfunction-associated fatty liver disease (MAFLD) has emerged as a valuable marker for identifying individuals at increased risk of metabolic dysfunction, liver-related complications, and cardiovascular disease. However, the association between MAFLD and testosterone deficiency (TD) in aging men remains poorly understood. This study aimed to investigate the association between MAFLD and the risk of TD in aging Taiwanese men, with a specific focus on those without metabolic syndrome (MetS).MethodsA free health screening program was conducted for Taiwanese men aged over 40 years in Kaohsiung, Taiwan. Participants underwent physical examinations, completed questionnaires regarding demographics, medical history, and clinical symptoms of TD, and provided 20-mL whole blood samples for biochemical, adipocytokine, and hormonal evaluations. Fatty liver index was used to evaluate the risk of fatty liver. Diagnostic criteria for MAFLD included fatty liver along with overweight/obesity, type 2 diabetes, or evidence of metabolic dysregulation.ResultsA total of 631 men (mean age: 54.4 ± 8.4 years) were enrolled. The prevalence rates of TD and MetS were significantly higher in men with MAFLD compared to those without (both p &lt; 0.001). Additionally, the presence of MAFLD showed a significant correlation with adipocytokines associated with insulin resistance, such as adiponectin, leptin, and retinol-binding protein-4 (RBP-4) levels (all p &lt; 0.001). Among men without MetS, those with MAFLD had a 3.89- and 4.74-fold higher risk of total testosterone &lt; 300 ng/dL and TD, respectively, after adjusting for potential covariates.ConclusionMAFLD is associated with an elevated risk of TD in aging Taiwanese men, particularly in the absence of MetS. This finding suggests that MAFLD could serve as an early predictor of TD, facilitating the identification of high-risk individuals and enabling timely interventions. Further research is needed to validate these findings and explore the underlying mechanisms linking MAFLD, TD, and MetS in diverse populations.
The leptin receptor has no role in delta-cell control of beta-cell function in the mouse
Authors: Zhang Jia , Katada Kay , Mosleh Elham , Yuhas Andrew , Peng Guihong , Golson Maria L. 
Published: October 2023

IntroductionLeptin inhibits insulin secretion from isolated islets from multiple species, but the cell type that mediates this process remains elusive. Several mouse models have been used to explore this question. Ablation of the leptin receptor (Lepr) throughout the pancreatic epithelium results in altered glucose homeostasis and ex vivo insulin secretion and Ca2+ dynamics. However, Lepr removal from neither alpha nor beta cells mimics this result. Moreover, scRNAseq data has revealed an enrichment of LEPR in human islet delta cells.MethodsWe confirmed LEPR upregulation in human delta cells by performing RNAseq on fixed, sorted beta and delta cells. We then used a mouse model to test whether delta cells mediate the diminished glucose-stimulated insulin secretion in response to leptin.ResultsAblation of Lepr within mouse delta cells did not change glucose homeostasis or insulin secretion, whether mice were fed a chow or high-fat diet. We further show, using a publicly available scRNAseq dataset, that islet cells expressing Lepr lie within endothelial cell clusters.ConclusionsIn mice, leptin does not influence beta-cell function through delta cells.
New perspectives on the evolutionary history of xiphosuran development through comparison with other fossil euchelicerates
Authors: Lustri Lorenzo , Antcliffe Jonathan B. , Saleh Farid , Haug Carolin , Laibl Lukáš , Garwood Russell J. , Haug Joachim T. , Daley Allison C. 
Published: October 2023

IntroductionEuchelicerata is a diverse group encompassing Xiphosura, Chasmataspidida, Eurypterida, and Arachnida. Xiphosura represents an extant group with a rich fossil record dating back to the Ordovician period. Xiphosurans are often referred to as “living fossils” due to their seemingly unchanged morphology over millions of years. Numerous studies have contributed to the understanding of xiphosuran development, revealing changes in the timing and rate of their growth. These changes have been mainly associated with the freshwater invasion of early xiphosuran forms. However, limited research has been conducted to compare the developmental patterns of xiphosurans with other euchelicerates inhabiting aquatic environments.MethodsThis study compares the developmental patterns of xiphosurans with that of the fossil clades of eurypterids and chasmataspidids. By incorporating environmental and phylogenetic information within ancestral state reconstruction analyses, and then testing different evolutionary scenarios, the influence of the environment on the evolution of developmental patterns of euchelicerates is examined.ResultsThe results confirm that the developmental changes in Xiphosura throughout their evolutionary history are correlated with the exploitation of different environments. However, the inclusion of eurypterids and chasmataspidids indicates that the entirety of changes seen for Xiphosura represent only a small portion of the total variability recovered for euchelicerates.DiscussionOur results emphasize the importance of considering phylogenetic relationships and outgroup comparisons to understand the evolutionary dynamics of Xiphosura.
Maternal satisfaction with intrapartum care and associated factors among mothers who gave birth in public hospitals of the South West Shewa Zone, Ethiopia, 2022
Authors: Tolesa Fikadu , Alemu Adugna , Beya Moges , Feyisa Mulugeta , Gezahagn Andualem , Negash Abdi , Shigign Erean , Getaye Asfaw , Negash Abraham , Merga Bacha 
Published: October 2023

BackgroundMaternal satisfaction with intrapartum care is a multidimensional assumption of satisfaction with self and with the physical environment of the delivery ward and quality of care. Maternal satisfaction with intrapartum care affects the selection of birthplace and helps to identify gaps between actual and intended healthcare outcomes. This study aims to assess factors that affect maternal satisfaction with intrapartum care.ObjectivesTo assess maternal satisfaction with intrapartum care and associated factors among mothers who gave birth in public hospitals in the South-west Shewa Zone, Ethiopia, 2022.MethodsA cross-sectional study approach among 420 mothers was conducted between April 14 and June 14, 2022. Systematic random sampling was used to select mothers for face-to-face interviews every two intervals. Bivariate and multivariable logistic regression analyses were carried out. P-values of &lt;0.25 in association with study variables were transferred to multivariable logistic regression models. An adjusted odds ratio with a 95% confidence interval was computed, and p-values of &lt;0.05 were considered statistically significant in the multivariable model. The results of this study are presented using text, tables, and charts.ResultsData were collected from 420 participants, and 413 mothers completed the interview, giving a response rate of 98.33%. The overall maternal satisfaction with intrapartum care was 245 (59.32%) [95% CI: 55–64]. Mothers who were considered normal during labor and delivery (AOR = 2.57 (95% CI: 1.30–5.07), had a labor duration of 12 h or less (AOR = 1.59 (95% CI: 1.03–2.44), and experienced a waiting time of &lt;15 min (AOR = 2.06 (95% CI: 1.21–3.52) were significantly associated with maternal satisfaction with intrapartum care.Conclusion and RecommendationsMore than half of mothers were satisfied with the overall intrapartum care they received. Health facility managers and healthcare providers work together to improve maternal satisfaction with intrapartum care.
Single-cell profiling reveals immune disturbances landscape and HLA-F-mediated immune tolerance at the maternal-fetal interface in preeclampsia
Authors: Luo Fangyuan , Liu Fulin , Guo Yingzhe , Xu Wenming , Li Yilin , Yi Jun , Fournier Thierry , Degrelle Séverine , Zitouni Hedia , Hernandez Isabelle , Liu Xinghui , Huang Yu , Yue Jun 
Published: October 2023

BackgroundPreeclampsia is a pregnancy-specific disorder that always causes maternal and fetal serious adverse outcome. Disturbances in maternal immune tolerance to embryo at the maternal-fetal interface (MFI) may be associated with preeclampsia onset. Recent studies have revealed the reduced expression pattern of HLA-F at the MFI in preeclampsia, while the mechanism of it mediating maternal fetal immune tolerance has not been revealed.MethodsSingle-cell RNA sequencing on placental decidua was performed to reveal the immune disturbances landscape at the MFI in preeclampsia. Human Jar cells and NK-92MI cells were employed to study the role of HLA-F in trophoblasts and lymphocyte.ResultsA total of 101,250 cells were classified into 22 cell clusters. Disease-related IGFBP1+SPP1+ extracellular villus trophoblast (EVT) was identified in the preeclamptic placental decidua, accompanied by newly discovered immune cellular dysfunction such as reduced ribosomal functions of NK populations and abnormal expression of antigen-presenting molecules in most cell clusters. Certain genes that are characteristic of the intermediate stage of myeloid or EVT cell differentiation were found to have unexplored but important functions in the pathogenesis of preeclampsia; specifically, we detected enhanced cell cross-talk between IGFBP1+SPP1+ EVT2 or SPP1+M1 cells and their receptor cell populations at the MFI of PE patients compared to controls. With respect to HLA-F, mIF staining confirmed its reduced expression in PE samples compared to controls. Over-expression of HLA-F in Jar cells promoted cell proliferation, invasion, and migration while under-expression had the opposite effect. In NK-92MI cells, over-expression of HLA-F increased the secretion of immunoregulation cytokines such as CSF1 and CCL22, and promoted adaptive NKG2C+NK cell transformation.ConclusionsWe revealed the immune disturbance landscape at the MFI in preeclampsia. Our findings regarding cellular heterogeneity and immune cellular dysfunction, as revealed by scRNA-seq, and the function of HLA-F in cells provide new perspectives for further investigation of their roles in the pathogenesis of preeclampsia, and then provide potential new therapeutic target.
Analysis of gut bacteriome of in utero arsenic-exposed mice using 16S rRNA-based metagenomic approach
Authors: Shukla Shagun , Srivastava Ankita , Verma Digvijay , Gangopadhyay Siddhartha , Chauhan Anchal , Srivastava Vikas , Budhwar Savita , Tyagi Dushyant , Sharma Deepak Chand 
Published: October 2023

IntroductionApproximately 200 million people worldwide are affected by arsenic toxicity emanating from the consumption of drinking water containing inorganic arsenic above the prescribed maximum contaminant level. The current investigation deals with the role of prenatal arsenic exposure in modulating the gut microbial community and functional pathways of the host.Method16S rRNA-based next-generation sequencing was carried out to understand the effects of in utero 0.04 mg/kg (LD) and 0.4 mg/kg (HD) of arsenic exposure. This was carried out from gestational day 15 (GD-15) until the birth of pups to understand the alterations in bacterial diversity.ResultsThe study focused on gestational exposure to arsenic and the altered gut microbial community at phyla and genus levels, along with diversity indices. A significant decrease in firmicutes was observed in the gut microbiome of mice treated with arsenic. Functional analysis revealed that a shift in genes involved in crucial pathways such as insulin signaling and non-alcoholic fatty liver disease pathways may lead to metabolic diseases in the host.DiscussionThe present investigation may hypothesize that in utero arsenic exposure can perturb the gut bacterial composition significantly as well as the functional pathways of the gestationally treated pups. This research paves the way to further investigate the probable mechanistic insights in the field of maternal exposure environments, which may play a key role in epigenetic modulations in developing various disease endpoints in the progeny.
Lactobacillus (Limosilactobacillus) reuteri: a probiotic candidate to reduce neonatal diarrhea in calves
Authors: Schwaiger Karin , Storch Julia , Bauer Christoph , Bauer Johann 
Published: October 2023

BackgroundDiarrhea in newborn calves is considered life-threatening and results in large economic losses in dairy farms. Lactobacilli generally play an important role in intestinal health, and Lactobacillus (Limosilactobacillus; L.) reuteri is the dominant Lactobacillus species in the feces of healthy calves during the first week of life. In calves with diarrhea on day 2 postpartum, lactobacilli are significantly reduced even up to 24 h before the onset of clinical signs. Since the probability of occurrence of diarrheal disease decreases as the L. reuteri count in the feces increases, oral administration of this species might have a protective effect against diarrhea.ObjectiveThese studies were designed to demonstrate whether oral administration of preselected L. reuteri isolates can reduce the incidence of diarrhea in newborn calves on dairy farms.Microorganisms46 L. reuteri isolates from 2-day-old healthy calves were available from a previous study.Animals170 newborn calves of Simmental breed of 10 dairy farms in Bavaria (Germany), were included in the study; of 166 animals the data could be evaluated.MethodsMicrobiological (antibiotic sensitivity test, acid and bile salt stability test, antimicrobial activity of the supernatants), molecular biological (PCR, RAPD-PCR) and toxicological methods (MTT test) were used to select and to characterize suitable L. reuteri isolates. The administration of a suspension of two selected L. reuteri isolates (6–8 × 108 colony forming units per day) to calves was performed from day 2 to day 5 after birth in a double-blinded placebo-controlled study. Clinical monitoring of the calves continued until the 14th day of life.ResultsOut of 46 L. reuteri isolates, only 2 met the set criteria and were used in the feeding trial. In the placebo group, 44 of 83 calves developed diarrhea within the first 2 weeks of life, whereas in the L. reuteri group this was only the case in 31 of 83 animals (p &lt; 0.05).ConclusionL. reuteri appears to be of particular importance for the intestinal health of newborn calves. The diarrhea protective effect could be even more pronounced if an improved administration regimen is developed in terms of start, frequency, and duration.