bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation and test of the reliability and validity of the Chinese defeat scale in the elite athletes of Chinese universities
Authors: Li Tao , Liu Yameng 
Published: January 2024

Abstract
Purpose
To test the reliability and validity of the "Defeat Scale" and explore whether the scale is suitable for use in surveys of college athletes.
Methods
A total of 226 athletes from five universities in China were selected for evaluation using the defeat scale. Factor analysis and correlation analysis were used to explore the reliability and validity of the defeat scale.
Results
Exploratory factor analysis showed that two factors could be extracted from this scale, namely a sense of decadence and a sense of low achievement. Confirmatory factor analysis showed that the model fit of the two factors was excellent (X2/DF = 2.809, RMSEA = 0.092, SRMR = 0.054, NFI = 0.891, IFI = 0.927, CFI = 0.926, TLI = 0.910). The convergent validity AVE values are 0.579 and 0.505 respectively, AVE > 0.5, and the combined reliability is 0.946 and 0.746 respectively. Both > 0.7 indicate good convergence effect. The arithmetic square roots of AVE, 0.760 and 0.710, are both higher than the absolute value of the correlation coefficient between the two dimensions, 0.30, indicating good discriminant validity; and the Cronbach's alpha coefficient of defeat is 0.931, and the Spearman-Brown coefficient is 0.888.
Conclusion
The defeat scale has good reliability and validity among college athletes.
Triiodothyronine is associated with incidence and resolution of fatty liver disease: a longitudinal study in euthyroid Korean adults
Authors: Kim Hye In , Kim Jun Young , Cho Jung Hwan , Han Ji Min , Suh Sunghwan , Bae Ji Cheol , Kim Tae Hyuk , Kim Sun Wook , Hahm Jong Ryeal , Chung Jae Hoon 
Published: January 2024

Abstract

The positive relationship between triiodothyronine (T3) and fatty liver demonstrated only in cross-sectional study. In this longitudinal cohort study, we aimed to evaluated whether total T3 (TT3) is associated with the development/resolution of fatty liver. We included 1665 South Korean euthyroid adults with ≥ 4 thyroid function tests. We explored the impact of TT3 average on development/resolution of either fatty liver (diagnosed by ultrasound) or modified metabolic dysfunction-associated fatty liver (MAFLD) using Cox proportional hazards regression models. During median 5 years follow-up, 891 (66.9%) participants among participants without fatty liver at baseline developed fatty liver, and 265 (79.6%) participants among participants with fatty liver at baseline resolved fatty liver. Compared with low TT3 average group, high TT3 average group was positively associated with development of fatty liver [adjusted HR 1.17 (1.03–1.34); P = 0.016] and inversely associated with resolution of fatty liver [adjusted HR 0.64 (0.50–0.82); P < 0.001]. The statistical significance was remained for development [adjusted HR 1.24 (1.07–1.44); P = 0.004] and resolution [adjusted HR 0.72 (0.54–0.95); P = 0.024] of modified MAFLD. Our finding provides longitudinal evidence that TT3 level was associated with development and resolution of either fatty liver or modified MAFLD.
Computational drug repositioning for IL6 triggered JAK3 in Rheumatoid Arthritis using FDA database
Authors: Banerjee Kaushani , Chandrasekhar Bavya , Sathish Sruthy , Sohn Honglae , Madhavan Thirumurthy 
Published: January 2024

Abstract

Rheumatoid Arthritis (RA) is a persistent autoimmune disease affecting approximately 0.5-1 percent of the world population. RA prevalence is higher in woman aged between 35 to 50 years than in age matched men, though this difference is less evident among elderly patients. The profound immune specific effects of disrupted JAK3 (Janus kinase 3) signaling highlight the possibility of therapeutic targeting of JAK3 as a highly specific mode of immune system suppression. To address the above problem which is unendurable to patients and in the hope to cater some respite to such suffering we have targeted JAK3 protein and JAK/STAT signaling pathway with compounds downloaded from FDA database, and performed screening of all available compounds docked against JAK3 protein. The difference between the target protein and other proteins of the same family was studied using cross docking and the compounds having higher binding affinity to JAK3 protein also showed more selectivity towards the particular protein. Density functional theory and molecular dynamics simulation study was done to study the compounds at their atomic level to know more about their drug likeliness. At the end of the study and based on our analysis we have come up with three FDA approved drugs that can be proposed as a treatment option for Rheumatoid Arthritis.
Effects of Electroacupuncture on Neural Function and Expression of Inflammation-Related Proteins NLRP3/Caspase-1 in Rats with Ischemic Stroke
Authors: Wang Jian , Xi Yujiang , Wang ZhiFeng , Xing LiWei , Zhang kun , Zhang Xiaoyu , Pan Pan 
Published: January 2024

Abstract
Objective:To investigate the neuroprotective effects of electroacupuncture at Baihui and Dazhui on rats in an ischemia-reperfusion injury model.
Methods: Forty-eight SPF male SD rats were randomly divided into sham operation group, model group, EA group and inhibitor group, with 12 rats in each group, and the middle cerebral arteryOcclusion (MCAO) model was established using the wire embolization method, and the model was successfully replicated and treated by acupuncture at Baihui and Dazhui. The neurological function scores were evaluated by Zea Longa neurological deficit score on days 1, 7 and 14 after treatment, and the volume of cerebral infarction was measured by 2, 3, 5-triphenyltetrazolium chloride staining. Detection of nod-like receptor protein 3 (NLRP3) and GSDMD gene expression by real-time fluorescence quantitative PCR , Western blot detection of NLRP3, GSDMD, Caspase-1 and other protein expression levels in rat brain tissue , and microglia levels were observed by immunofluorescence staining; the expression levels of interleukin-1β (IL-1β) and interleukin-18 (IL-18) were detected by enzyme-linked adsorption assay.
Results:Compared with the model group, the rats in the electroacupuncture group had significantly lower neurological deficit score (p < 0.05), significantly reduced brain infarct volume (p < 0.05), significantly reduced NLRP3 and GSDMD gene expression (p < 0.05), reduced NLRP3, GSDMD, Caspase-1 and other protein expression water, IL-1β, IL-18 inflammatory factor expression were significantly reduced (p < 0.05).
Conclusion: Electroacupuncture can improve the symptoms of neurological injury in rats with ischemic stroke model, and may play a neuroprotective role by inhibiting NLRP3/Caspase-1 inflammatory pathway and reducing the expression of cell scorch-related proteins.
Accident Frequency Analysis Using Two-Step Algorithm at Penamaluru and Gosala intersections on NH-65 – A Case Study Vijayawada City
Authors: Narra Malathi , Bavineni Pandu Ranga Rao , Mummaneni Shobana , Ventrapragada Hari Chandana , Kammila Naga Priyanka 
Published: January 2024

Abstract
four interconnected conditions: human behavior, vehicle efficiency, and infrastructure characteristics. This project aims to compile accident data from respective police stations, analyze the acquired information, pinpoint blackspot regions through field studies, and propose measures to mitigate accident severity. The analysis of traffic accidents encompasses factors such as vehicle type, location (urban or rural), age, gender, driver behavior, alcohol consumption, overloading, and other relevant considerations. Statistical procedures like frequency analysis, clustering using a two- step algorithm, and the ASI are employed to identify blackspot areas based on the collected accident data. Individual field examinations include inventory investigations, traffic studies like volume and speed assessments, and geometric investigations. Ultimately, the analysis and field research culminate in the proposal of highway safety measures aimed at reducing accident severity in blackspot areas through the implementation of both physical and non-physical engineering measures, with the goal of enhancing road traffic safety and smoothness in the foreseeable future.
Long-term combined “new sports” and resistance exercise improves the physical and mental health of elderly people with stroke-associated hemiplegia
Authors: Lee Ji-Eun , Akimoto Takayuki , Lee Ho-Seong 
Published: January 2024

Abstract

In this study, we examined the effects of 24 weeks of a combined “new sports” and resistance exercise program on the physical and mental health of elderly people with hemiplegia. Thirty elderly people aged 65–74 years with hemiplegia were randomly divided into combined new sports and resistance exercise (NS+RE), resistance exercise (RE), and control groups. Indicators of physical health (range of motion [ROM], Berg Balance Scale [BBS], Functional Gait Assessment, and Instrumental Activities of Daily Living) and mental health (Grooved Pegboard Test [GPT], Geriatric Depression Scale [GDS], Rosenberg Self-Esteem Scale [RSES], and Stroke-Specific Quality of Life [SS-QOL]) were measured before and at 8, 16, and 24 weeks of exercise training. Compared with the RE and control groups, the NS+RE group showed significant improvements in ROM, RSES, and SS-QOL starting at 8 weeks, GPT starting at 16 weeks, and BBS and GDS at 24 weeks. We conclude that combined new sports and resistance exercise was more effective than resistance exercise alone in terms of improving the physical and mental health of elderly people with hemiplegia. Our findings highlight the importance of integrating new sports into rehabilitation strategies and sustaining long-term exercise for lasting benefits.
Identification and Functional Analysis of Rare HECTD1 Missense Variants in Human Neural Tube Defects
Authors: Oxman Elias , Li Huili , Wang Hong-Yan , Zohn Irene 
Published: January 2024

Abstract
Neural tube defects (NTDs) are severe malformations of the central nervous system that arise from failure of neural tube closure. HECTD1 is an E3 ubiquitin ligase required for cranial neural tube closure in mouse models. NTDs in the Hectd1 mutant mouse model are due to the failure of cranial mesenchyme morphogenesis during neural fold elevation. Our earlier research has linked increased secretion of extracellular heat shock protein 90 (eHSP90) to aberrant cranial mesenchyme morphogenesis in the Hectd1 model. Furthermore, overexpression of HECTD1 suppresses stress-induced eHSP90 secretion in cell lines. In this study, we report the identification of five rare HECTD1 missense sequence variants in NTD cases. The variants were found through targeted next-generation sequencing in a Chinese cohort of 352 NTD cases and 224 ethnically matched controls. We present data showing that HECTD1 is a highly conserved gene, extremely intolerant to loss-of-function mutations and missense changes. To evaluate the functional consequences of NTD-associated missense variants, functional assays in HEK293T cells were performed to examine protein expression and the ability of HECTD1 sequence variants to suppress eHSP90 secretion. One NTD-associated variant (A1084T) had significantly reduced expression in HEK293T cells. All five NTD-associated variants (p.M392V, p.T801I, p.I906V, p.A1084T, and p.P1835L) reduced regulation of eHSP90 secretion by HECTD1, while a putative benign variant (p.P2474L) did not. These findings are the first association of HECTD1 sequence variation with human disease and suggest that sequence variation in HECTD1 may play a role in the etiology of human NTDs.
Analysis of the influence of different rock (soil) bodies on the failure characteristics of overlying rock in Yushen mining area
Authors: Zhang Fengda 
Published: January 2024

Abstract
In order to analyze the influence of different rock ( soil ) bodies on the failure mode of overburden rock in Yushen mining area, three types of overburden rock structures, including red soil-weathered bedrock-normal bedrock, weathered bedrock-normal bedrock and normal bedrock, were selected in the range of mining-induced cracks. The development degree of mining-induced cracks in soil layer and bedrock was observed by water pressure test method and leakage observation method respectively, and the failure height and saddle-shaped alienation characteristics of overburden rock under different rock (soil) structures were compared and analyzed. The results show that when there is red soil or weathered bedrock near the top of mining-induced cracks, the ratio of the failure height of mining-induced overburden to the thickness of coal seam mining is between 9.95 ~ 11.65 and 12.21 ~ 15.55, respectively, which is significantly smaller than that of normal bedrock (18.10 ~ 19.41). Red soil layer or soft rock overburden is helpful to inhibit the development of mining-induced cracks. The greater the difference between the compressive strength, tensile strength and shear strength of the rock mass near the top of the mining crack, the more obvious the 'saddle-shaped' alienation characteristics of the overlying rock failure under the influence of mining. Compared with the weak or very weak type of overburden failure range, the overburden failure range corresponding to different rock (soil) structures in Yushen mining area is obviously larger.
Differentially expressed miRNAs in db mouse induced diabetic cardiomyopathy
Authors: Yao Ju , Zhang Kang , Zhao Rong , Yang Xiaoyu , Wang Hui 
Published: January 2024

Abstract
With the increasing prevalence of diabetes mellitus, diabetes mellitus-induced diabetic cardiomyopathy was one of the common complications of diabetes and the main cause of sudden cardiac death in diabetic patients. The pathogenesis of diabetic cardiomyopathy is not well understood. We discussed the potential miRNAs and pathways in progression of cardiac function impairment in diabetic cardiomyopathy. In our study, we used microarray analysis to obtain 48 abnormally expressed miRNAs in 16 - and 24-week db mouse induced diabetic cardiomyopathy models, of which 23 upregulated expressions and 25 downregulated expressions. Subsequently, the downstream target genes of these abnormal miRNAs were screened and 3079 target genes were obtained. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed for these abnormally expressed genes. This study provides a new way to further explore the molecular mechanism of diabetic cardiomyopathy, and has important guiding significance for elucidating the pathogenesis and the development of prevention and treatment drugs.
Psychometric Evaluation of the Caring Ability of the Family Caregivers of the Patients in Need of Palliative Care Scale (CAFCPNPCS): A Methodological Study
Authors: Ashrafizadeh Hadis , Farahani Azam Shirinabadi , Karami Maryam , Khademi Fatemeh , Dorcheh Azam Eshaghian , Mojen Leila Khanali , Nasiri Maliheh , Rassouli Maryam 
Published: January 2024

Abstract

Background
Since family caregivers play an important role in providing care for the patients in need of palliative care, it is important to focus on and examine their care ability in fulfilling the expected roles. Therefore, this study was conducted with the aim of performing a psychometric evaluation of the Caring Ability of the Family Caregivers of the Patients in Need of Palliative Care Scale (CAFCPNPCS).
Methods
The present study is methodological research that evaluates the psychometric characteristics of the CAFCPNPCS by measuring face validity, content validity, construct validity (confirmatory factor analysis and divergent validity) and internal consistency in 412 family caregivers of the patients in need of palliative care, who were selected through convenient sampling. The data were analyzed using SPSS 24 and LISREL 8.8.
Results
According to the confirmatory factor analysis, the 5-factor model with 31 items was confirmed with the values RMSEA = 0.05, CFI = 0.95 and GFI = 0.88. The divergent validity was measured by estimation of correlation between the caring ability score with care burden score (which was measured by Zarit Burden Interview) of the family caregivers of the patients in need of palliative care. Cronbach's alpha (α) was reported to be 0.846 for the whole scale and 0.781–0.852 for other dimensions.
Conclusion
CAFCPNPCS is a valid and reliable tool that can evaluate the caring ability of the family caregivers of the patients in need of palliative care. Based on the results, it is recommended to utilize this scale to determine these caregivers’ ability in providing care and to examine the effectiveness of interventions with the aim of improving the dimensions of their caring ability.
The combination of single-cell and bulk RNA sequencing analysis decodes the melanoma tumor microenvironment and identifies novel T cell-associated signature genes
Authors: Luo Sihan , Wang Daiyue , Chen Jiajie , Hong Shaocheng , Fang Yuanyuan , Cao Lu , Yong Liang , Liu Shengxiu 
Published: January 2024

Abstract
Background and aim: Skin cutaneous melanoma (SKCM) is a fatal type of skin cancer that arises from the malignant conversion of melanocytes. Different melanoma patients show various immunotherapy responses, which may involve many immunotherapy-related genes in T cells (IRTGs). This study aims to construct a risk score model that can predict the prognosis and immunotherapy response of SKCM patients.
Methods: Our study conducted single-cell RNA sequencing (scRNA-seq) analysis on many melanoma patients from several public datasets. Herein, we screened candidategenes in the T cells of melanoma tumors that were highly associated with immunotherapy response. The candidate genes underwent LASSO and COX regression analyses to identify the signature genes. The immune activity was assessed in patients with different scores using ESTIMATE and CIBERSORT algorithms. Finally, the signature gene PEB4B and the immune microenvironment correlation was explored with multiple immunofluorescence (IF) and well-established biological algorithms.
Results: Of the initial 33 candidate genes, we successfully identified six distinct signature IRTGs. Additionally, the computation of each patient risk score proved beneficial in evaluating the immune cell infiltration level and functions as an independent prognostic factor for melanoma patient survival. The risk score results revealed promising predictive outcomes in determining the response of melanoma patients to immunotherapy.
Conclusion: Our findings demonstrate the potential utility of a risk score dependent on signature genes as a predictive tool for assessing the prognosis and response to immunotherapeutic interventions in melanoma patients.
Genetic variations, clinical presentation and treatment outcome of isolated growth hormone deficiency type I and II: case series and review of the literature
Authors: Li Ran , Yang Yiying , Bao Xudong , Chen Meiping , Yang Hongbo , Gong Fengying , Du Hanze , Pan Hui , Zhu Huijuan 
Published: January 2024

Abstract

Purpose: To report a case series of four patients with isolated growth hormone deficiency (IGHD) type I from two Chinese pedigrees and to elucidate phenotype-genotype correlation of IGHD type I and II with GH1 gene alterations in the literature.
Methods: Whole exome sequencing (WES) were performed and a literature review was conducted.
Results: Four patients presented with extreme growth retardation (height -4.74 to -6.50 SDS) and undetectable peak growth hormone (GH) during GH stimulating test. WES revealed a novel homozygous nonsense mutation, c.316delC (p.L106Cfs*35), in GH1 gene in the the first pedigree. Deletions of exon 1-5 in GH1 gene were identified in the second pedigree. Ideal catch-up growth after GH treatment was achieved. 94 patients with IGHD type I and 240 patients with IGHD type IIwere included in literature review. Patients with IGHD type I exhibited younger age (3.2 vs 6.0 years, P<0.001), more severe growth retardation (median height -6.50 vs -3.84 SDS, P<0.001), lower peak GH levels (0.05 vs 1.70 ng/ml, P<0.001) and a higher dosage of GH (0.22 vs 0.17 mg/kg/week, P=0.012) compared to patients with IGHD type II. Gross deletions constituted 72.3% of IGHD type I cases, while splicing mutations and missense mutations comprised 54.2% and 45.0% of IGHD type II cases. Height SDS after GH treatment in IGHD type II and in patients with mutations affecting splicing was negatively associated with age at diagnosis.
Conclusion: IGHD type I is a rare inherited disease. Earlydiagnosis and initiating treatment may lead to a better prognosis.
Individual risk stratification to predict metastasisfor ESD of superficial esophageal squamous cell carcinoma
Authors: Jiang Dongxian , Chen Weijie , Zhang Xue , Song Qi , Wang Haixing , Huang Jie , Huang Jieakesu , Shen Licheng , Xu Chen , Hou Yingyong 
Published: January 2024

Abstract
Purpose
This study was aimed at identifying histopathological risk factors for metastasis and developing a reliable risk stratification system for superficial esophageal squamous cell carcinoma (sESCC) after endoscopic submucosal dissection (ESD).
Methods
Three hundred and eight patients who underwent ESD from January 2009 to December 2019, and were pathologically confirmed as mucosal or submucosal cancer were included. Their clinicopathological characteristics were reviewed.
Results
The median follow-up period was 51 months (range 12–132 months). We found 42 patients had recurrence, 16 metastasized (1 distant metastasis, 13 lymph node metastasis, 2 both distant and lymph node metastasis), and 23 patients died. This univariate analysis revealed five morphological features were significantly associated with metastasis and overall survival. They were invasive depth, tumor budding, differentiation, infiltrative growth pattern, and vessel invasion(P < 0.05). Vessel invasion (hazard ratio 3.982, 95% confidence interval 1.273–12.456) was confirmed with multivariate analysis as independent risk factor for metastasis.The five factors were included in the risk stratification system and assigned scores (0–5). With the cutoff value of 2.5 score, all cases were re-classed into a group of 275 low-risk tumors, and a group of 33 high-risk tumors. Our data revealed the metastasis rates of patients in high-risk group were significantly higher than those in low-risk group (P < 0.001).
Conclusions
Based on histopathological characteristics of ESD specimens, we developed a risk stratification system, which significantly divided sESCC into low-risk and high-risk group for metastasis. This may be used to assist in the decision-making process of sESCC to determine who need additional therapy after ESD.