bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomics and metabolomics analysis of Cormus domestica (L.) fruits and the valorisation of an ethnobotanical heritage of culinary and medicinal uses in Mediterranean area.
Authors: Tartaglia Maria , Zuzolo Daniela , Prigioniero Antonello , Ranauda Maria Antonietta , Scarano Pierpaolo , Tienda-Parrilla Marta , Hernandez Tamara , Jorrín-Novo Jesús , Guarino Carmine 
Published: January 2024

Abstract

Background
Cormus domestica (L.) is a monophyletic wild fruit tree belonging to the Rosaceae family, with well-documented use in the Mediterranean region. Traditionally, these fruits are harvested and stored for at least 2 weeks before consumption. During this period, the fruit reaches its well-known and peculiar organoleptic and texture characteristics. However, the spread of more profitable fruit tree species, resulted in its progressive erosion. In this work we performed proteomic and metabolomic fruit analyses at three times after harvesting to provide data on its chemical composition, nutritional and nutraceutical properties.
Results
Proteomic and metabolomic analyses were performed at three ripening stages: freshly harvested fruit (T0), fruit two weeks after harvest (T1) and fruit four weeks after harvest (T2). Proteomic analysis (Shotgun Proteomic in LC-MS/MS) resulted in 643 proteins identified. Most of the differentially abundant proteins between the three phases observed were involved in the softening process, carbohydrate metabolism and stress responses. Enzymes, such as xyloglucan endotransglucosylase/hydrolase, pectin acetylesterase, beta-galactosidase and pectinesterase, accumulated during fruit ripening and could explain the pulp breakdown observed in C. domestica. At the same time, enzymes abundant in the early stages (T0), such as sucrose synthase and malic enzyme, explain the accumulation of sugars and the lowering of acidity during the process. The metabolites extraction from C. domestica fruits enabled the identification of 606 statistically significant differentially abundant metabolites. Some compounds such as piptamine and resorcinol, well-known for their antimicrobial and antifungal properties, and several bioactive compounds such as endocannabinoids, usually described in the leaves, accumulate in C.domestica fruit during the post-harvest process.
Conclusions
The C. domestica fruit metabolomic and proteomic profiling during the post-harvest process showed in the study, fills an information gap and has enabled the molecular and phytochemical characterisation of this erosion-endangered fruit. Data support the nutritional and nutraceutical value of this species.
Lithometeors and public health issue in the city of Maroua (Far North Cameroon)
Authors: BOUBA Lucas , Kemo William , Assomo Philippe Samba 
Published: January 2024

Abstract

The Far North region of Cameroon is subject to significant dust storm aerosol concentration, leading to deterioration in urban air quality. The temporal monitoring of aerosols and the assessment of their impacts on human health were carried out in the city of Maroua, through a field campaign resulting in the collection of in situ dust aerosols data and those relating to epidemiology obtained from the Maroua Regional Hospital. Four aerosol samples were also submitted to Laboratory chemical analysis. The results of the present study have shown first of all that there are strong correlations between the temporal evolution of aerosol episodes and the prevalence of respiratory diseases. The period from January to march is positioned as the high-risk pollution period. Chemical analysis of the aerosols revealed the presence of potentially toxic elements such as threshold pollutants (Lead, Cadmium, and Cyanide) and non-threshold pollutants (Potassium, Sodium, Sulfate and chloride). The concentrations of the chemical elements were observed in the following order: SO42- > K+ > Na+ > Ca2+ > Cl- > Mg2+ > NH4+ > NO3- > F- > Pb > Cd > CN-; the presence of PO43- and As not having been detected. The exposure of the human population to these potentially toxic elements from inhalation pathway suggested an acceptable non-carcinogenic risk and a very low carcinogenic risk.
Molecular cytogenetic characterization and field performance analysis of alien chromosome addition lines in five generations of Erianthus x Saccharum
Authors: V.P. Sobhakumari , K Mohanraj 
Published: January 2024

Abstract
Erianthus arundinaceus, one of the species of ‘Saccharum complex’ has a number of important agronomic traits including good ratooning ability, tolerance to both drought and waterlogging, disease resistance and vigor and is of interest as a potential source of parental germplasm to sugarcane breeders. We report here for the first time the chromosome composition, Erianthus chromosome transmission patten and agronomical trait evaluation of Erianthus addition lines in five consecutive generations of E. arundinaceus x Saccharum. The hybridity of randomly selected clones could confirm with ErianthusSpecific Tandem Repeat (ESTR) sequences. The results of classical cytology revealed that the mode of transmission of gametes, except in the second generation, followed n+n pattern whereas in second generation (CYM 07-971) it was showing 2n+n with elimination of few chromosomes. Progressive elimination of Erianthuschromosomes is observed in consecutive generations where the F1 showed 30 Erianthuschromosomes and it was ranged from 0-2 in fifth generation. Agronomical trait analysis indicates that further backcrossing with commercial clones with high juice quality or intercrossing among the selected progenies would improve both juice quality and cane traits in E. arundinaceus x Saccharum hybrids.
Microenvironment Evolution at Triple-phase Interface on the CO2RR Process of Hydrophobic Oxide-derived Copper
Authors: YU Jinli , Xie Zezhong , Yang Hao , Wang Qiushi , Chen Jian , Song Shu-qin , Meng Changgong , Wang Kun , Tong Yexiang 
Published: January 2024

Abstract
The local microenvironment of electricity-powered CO2 electroreduction reaction (CO2RR) surrounding the catalyst-electrolyte-gas triple-phase interface plays a crucial role in catalytic activity and selectivity as it affects reaction pathways and species transport. However, it still needs to be explored and understood regarding the impact of microenvironment evolution on the CO2RR performance. We report here a hydrophobic oxide-derived copper foam with villous nanowires on the surface that demonstrates significant suppressed HER and enhanced C2+ selectivity in H-type cell. In-situ 3D Raman mapping and in-situ Raman spectra investigation on micro-environmental species reveal that high local pH and fast CO2 mass transfer were simultaneously allowed in the microenvironment of the triple-phase interface because of the special hydrophobic structure. On this mechanism, the material reaches a minimum H2 Faradaic efficiency (FE) of 6.6% and maximum C2+ FE of 74.4% at the current density of 300 mA cm-2 in a flow cell under acidic conditions (pH=4) without an additional gas-diffusion layer (GDL). This study not only highlighted the importance of the microenvironment but also provided an effective method for tuning the triple-phase interface of CO2RR and demonstrated a promising application of the pure metal foam-based GDEs.
Pathways of Various Toxic Materials from Open Landfill Site and Their Assessment
Authors: Pant Deepak , Vaid Akash , Singh Umang , Sharma Vrinda , Sharma Sonali , Shera Pandit Prashant , Giri Anand , Kumar Anil 
Published: January 2024

Abstract
Toxic assessment study of various chemical pollutants plays a vital role towards its long-term environmental impact. Open landfill site carry many toxic pollutants in the water system like, methyl-thiourea; 3,5-dinitro- benzamide; n-trifluoroacetylmorpholine; di-n-ethylhexyl phthalate; ethyl-trans-hex-3-enylester phthalic acid; 2,4,-dimethyl thiazole; anthraquinone 1-amino-4-benzamido and alizarin red S. LC-MS results confirm the presence of these contaminants, many of which may have evolved due to various hydrolysis and photolysis reactions between production and source. This problem becomes quite complex at landfill sites in hilly areas. This study is reporting various primary and secondary pollutants in the river water system as a result of open dumping mismanagement. Pollutants are classified based on their potential sources such as pharmaceutical, herbicide and pesticide (PHP), food packaging and flavouring (FPF) and dye waste.
Plant endophytic fungi exhibit diverse biotransformation pathways of mogrosides and show great potential application in siamenoside Ⅰ production
Authors: Lin Wenxi , Jiang Qiang , Dong Yamin , Xiao Yiwen , Wang Ya , Gao Boliang , Zhu Du 
Published: January 2024

Abstract

Fungal endophytes, as an untapped resource of glycoside hydrolase biocatalysts, need to be further developed. The primary active compound in the fruit of Momordica grosvenorii, mogroside V, can be converted into other various bioactive mogrosides by selective hydrolysis of glucose residues at C3 and C24 positions. In present study, 20 fungal strains were randomly selected from our endophytic fungal strain library to investigate their capability for transforming mogroside V. The results revealed that relatively high rate (30%) endophytic fungal strains exhibited the ability of transformation. Further analysis indicated that endophytic fungi could produce abundant mogrosides, and the pathways for biotransforming mogroside V showed diverse. Among the given fungal endophytes, Aspergillus sp. S125 could almost completely transform mogroside V into the end-products mogroside II A and aglycone only after 2 days of fermentation; Muyocopron sp. A5 produced rich intermediate products, including siamenoside Ⅰ, and the end-product mogroside II E. Furthermore, Aspergillus sp. S125 and Muyocopron sp. A5 were selected to optimize the fermentation conditions in order to evaluate the feasibility of large-scale conversion of mogroside V. After optimization, Aspergillus sp. S125 could convert 10 g/L of mogroside V into 4.5 g/L of mogroside II A and 3.6 g/L of aglycone after 3 days of fermentation, while Muyocopron sp. A5 could selectively produce 4.88 g/L of siamenoside Ⅰ from 7.5 g/L of mogroside V after 36 hours of fermentation. This study not only provides a class of highly effective biocatalytic candidates for transform mogrosides, but also strongly indicates that plant endophytic fungi can be used as a potential resource for biocatalysis of natural compounds.
The Effect of Supportive Care on Mood, General Health, Competence, and Supportive Care Needs on Caregivers of Cancer Patients Undergoing Chemotherapy: A Controlled Clinical Trial
Authors: miri Foroogh , karimyar Mahdi , Hojat Mohsen 
Published: January 2024

Abstract
Background: most cancer patients live more than 5 years after the diagnosis and many of them rely on their informal caregivers. The quality of life and survival of cancer patients depend on their caregivers' abilities.
Methods: This study aimed to assess the effectiveness of a supportive care program on mood, general health, competence, and the supportive care needs of caregivers of cancer patients undergoing chemotherapy. A randomized controlled trial was conducted in the south of Iran. The caregivers were randomly assigned to experimental (30) and control (30) groups. The data were measured using the Profile of Mood States, General Health Questionnaire, and Caregiver Competence Scale at baseline, immediately, one month after intervention. Too, the Supportive Care Needs questionnaire was filled out by patients at baseline, immediately, one month after intervention. Data was analyzed by the IBM SPSS 16.0. Kolmogrov-Smironov test, Repeated Measurement test, chi-square test, Friedman test, Manwitney U, and Independent T-test were used.
Discussion: The average scores for mood, general health, competence, and supportive care needs of caregivers in the experimental group improved compared to the control group immediately and one month after the intervention (P < .05). The administration of the supportive care program positively affected the mood, general health, competence, and supportive care needs of caregivers.
Trial registration: Clinical trial registration number: (IRCT20230128057252N1) https://en.irct.ir/trial/69729, date of registration: (2023-07-08)
Construction of Fish Vaccine via Heterologously Biosynthesizing Bacterial Type III Secretion System
Authors: hu shengbiao 
Published: January 2024

Abstract
The delivery of antigens into the host cells through type III secretion system (T3SS) is an effective strategy employed by bacteria to induce host immune responses and facilitate colonization. To achieve optimal immersion protection with a vaccine, we employed a T3SS antigens delivery system, which is heterologously biosynthesized in an avirulent Aeromonas salmonicida DBFF01, to deliver heterologous antigen as a vaccine carrier. A recombinant GAPDH (rGAPDH) protein, which was fused with a Photorhabdus luminescens TT01 translocation signal and chaperone SlcT, was constructed as an antigen. Then, we investigated the extracellular secretion of heterologous expression rGAPDH, as well as its expression during the infection of Hela cells. Western blotting demonstrated that heterologously biosynthesized T3SS could exhibited functional activity. More importantly, T3SS-expressing A. salmonicida strains had no toxicity or side effects when tested with hematoxylin and eosin assays. The immune protection of DBFF01-T3SS-Δphage-rGAPDH and DBFF01-T3SS-rGAPDH against the pathogen E. tarda in grass carp was evaluated at 59.1% and 37.4% by the relative percent survival (RPS), respectively. In addition, we analyzed the expression profiles of multiple immune-related genes after challenge. Notably, complement component 3 (C3) was up-regulated in the livers of grass carp injected with T3SS-expressing A. salmonicida strains, indicated that C3 may play an assisting role in repairing liver tissue damaged by E. tarda. In conclusion, our study found that T3SS is an effective antigen delivery system, which employed the type III secretion system to deliver recombinant immunogenic protein rGAPDH in grass carp against E. tarda, and we suggested that the vaccine could induce corresponding immune responses and facilitate colonization in fish.
A 11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) inhibitor, 11b-0048, effectively suppresses the expression of 11β-HSD1 activated in cultured keratinocytes and in diabetic murine skin
Authors: Lee Ju Yeong , Heo Yeon-Woo , Hwang Hyun Jee , Kim Eunjung , Lee Jee-Young , Kang Seunghyun , Choi Eung Ho 
Published: January 2024

Abstract

Elevated level of active glucocorticoid (GC) deteriorates skin barrier function. 11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) is an NADPH-dependent enzyme converting inactive GC to active GC. Elevated active GC due to increased 11β-HSD1 expression might contribute to barrier impairment in aged skin and diabetic skin. We believe that the increase of 11β-HSD1 expression is a main cause of barrier abnormalities in diabetic skin and perform this study to elucidate the effect of a new 11β-HSD1 inhibitor. We compared it with a proven inhibitor in the cultured keratinocytes inducing typically 11β-HSD1 activation with dexamethasone treatment, UVB irradiation, and high glucose treatment, and the db/db mice as a type 2 diabetes murine model. In the cultured medium, cortisol, 11β-HSD1, and cytokines were measured. Also, in the db/db mice with a two-week application of 11β-HSD1 inhibitors, skin barrier function, HbA1c, corticosterone, 11β-HSD1, and cytokines were measured. In cultured keratinocytes, all concentrations and mRNA levels of cortisol, 11β-HSD1, and cytokines were decreased by both 11β-HSD1 inhibitors. In the db/db mice, both inhibitors improved skin barrier function and reduced serum level of HbA1c and skin expression of corticosterone, 11β-HSD1, and cytokines. A new 11β-HSD1 inhibitor, “11b-0048”, showed a significant inhibitory effect on the expression of 11β-HSD1 in keratinocytes activated by various conditions and diabetic skin.
Shortened Survival of Chronic Hemodialysis Patients during Quarantine Periods of COVID-19. A Retrospective Analysis of 344 Patients
Authors: Oppenheim Amit , Gueta Itai , Haj Ramy , Abu-Amer Nabil , Ozeri David Joshua 
Published: January 2024

Abstract
Background. COVID-19 pandemic impact on patients’ populations could be divided to direct consequences of the disease and indirect implications of changes imposed upon the national healthcare systems, e.g., large-scale quarantines. The impact of the later on survival of chronic hemodialysis patients is still unknown.
Methods. We employed a retrospective, case-controlled study comparing a population of chronic hemodialysis patients from two separate time-frames: during a quarantine period and a parallel time without quarantine.
Results. This study included 344 hemodialysis patients, baseline characteristics were compared between those enrolled in 2015 and 2020. Despite comparable clinical parameters, a statistically significant increase in the 6-month mortality rate was observed in 2020 (1.2% to 6.7%, p = 0.01), primarily attributed to sepsis. Notably, no deaths were attributed to COVID-19 in 2020. Interdialytic weight gain and dialysis quality remained similar between the two groups, with a mild trend towards excessive weight gain in 2020. Secondary outcomes after 6 months did not significantly differ, except for lower sodium values in 2015 compared to 2020.
Conclusions. In this study, dialysis patients experienced disproportionate effects from the COVID-19 pandemic, even with continuous care and no direct virus-related fatalities. The findings unveil unintended consequences of quarantine measures, highlighting adverse impacts on both physical and mental health. Recognizing and addressing these consequences are imperative for minimizing their impact in future pandemics, emphasizing the importance of proactive measures in healthcare planning.
Platelet count-to-platelet distribution width ratio and other platelet indices as cost-effective markers of preeclampsia: a case control study
Authors: Bashyal Ritu , Sharma Paban Kumar , Singh Alka , Maharjan Sanjay , Tuladhar Shreyashi , Bhattarai Binita 
Published: January 2024

Abstract

Background
Platelet indices, like platelet count (PC), plateletcrite, mean platelet volume (MPV) and platelet distribution width (PDW), and their ratios have shown to be a cost-effective and better predictors of preeclampsia (PE). However, the PC/PDW ratio was not studied. Thus, this study aimed to compare platelet indices and their ratios between pregnant women with and without PE.
Methods
An analytical, comparative, case-control study was conducted. Pregnant women of 20 weeks gestation or more were categorized into either case (with PE) or control (without PE) group. The differences between cases and controls were compared using Student’s t-test and Mann‒Whitney U-test for the continuous variables and chi-square tests and Fisher’s exact test for categorical variables. Multivariable linear regression analysis for hematological parameters was performed to assess the effect of gestational age. Logistic regression was performed to calculate the odds ratio. A receiver operating characteristic curve was used to analyze the sensitivity and specificity of the tests and to find significant cutoff values to predict the presence of PE. The level of significance (α) was set at 0.05.
Results
A total of 24 cases and 72 controls were enrolled. There was a statistically significant reduction in the values of PC (p=0.004), plateletcrit (p=0.012), PC/MPV ratio (p=0.002), and PC/PDW ratio (p= <0.001) among the pregnant women with PE compared to the control study group, while a significant increase was noticed in the values of PDW (p= <0.001) and MPV (p=0.021).
PC/PDW had the highest area under the curve (AUC) of 0.767, followed by PDW (AUC=0.752). At the cutoff value of 15.1 (p<0.001) for PC/PDW, the sensitivity was 70.8%, and the specificity was 81.9%. The odds of diagnosing true positive cases of PE was 11.02 (95% CI =3.79-31.99, p=<0.001) times higher compared to values below it at this cutoff point.
Conclusions
The present study suggests that platelet indices are economical tests that can act as indicators of risk of PE. Among all the parameters, PC/PDW has the highest sensitivity and specificity in the detection of PE at the cutoff of 15.1 and has emerged as a better predictor of PE.
Effect of thoracic endovascular aortic stent diameter on short term mortality in esophageal cancer-associated aortoesophageal fistula
Authors: Chung Mengwei , Cheng Kanglun , Kao Peiyu 
Published: January 2024

Abstract
This case series presents a single center experience on treating esophageal cancer related aortoesophageal fistula (AEF) .
From January 2010 to October 2022, 7 patients with cancer related AEF were treated with thoracic endovascular aortic repair (TEVAR) at China medical university hospital.
Main tumor localized at the middle to lower third of esophagus, with residual disease or local recurrence disease status was the main risk factor of aortoesophageal fistula (AEF) development.
Survival after initial successful endovascular treatment ranged from 17 to 111 days. 4 patients died of non-aortic-related death. 3 of them survived more than 2 months and are able to discharge without disability. Thus, to reduce aortic related death is a crucial topic. Choosing an ‘extra’ oversizing stent graft selection up to 120-130% aortic diameter may produce better surgical outcome. As for late mortality after discharge, infection may play the most important role.
Analysis of the rainfall variability over temporal and spatial patterns: A case study in Adelaide, South Australia
Authors: To Hoan , Ahammed Faisal 
Published: January 2024

Abstract
Climate change has presented a tremendous impact on the weather patterns on Earth. Many studies conducted to investigate the changing patterns of meteorological data in Australia. This study aimed to investigate the variability of rainfall data over time and terrain in Adelaide, South Australia. The annual daily maximum rainfall (ADMR) data over a 40-year period in four stations was collected to identify the distribution of rainfall data across the time recorded. Moreover, the ADMR data in 2018 and elevation data across 86 stations were used to investigate the changing rainfall patterns over the terrain. Two non-parametric tests including Kruskal–Wallis, and Mann–Whitney were applied to perform the hypothesis analysis. Correlations, regression, and multivariate tests were performed to identify the relationship between variables. It was found that the ADMR data in four stations did not vary over the 40-year period from 1981 in Adelaide. However, there was a strong correlation between the extreme rainfall data in the year 2018 and elevation data in these stations. Results also suggested that it is relatively possible to use the elevation data to predict ADMR across Adelaide in certain years. Policymakers and researchers can use these tests for climate projections and extreme rainfall forecasts.