bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shoulder reduction on the scene: current practice and outcome of the Bavarian Mountain Rescue Service—a prospective observational study
Authors: Woyke Simon , Pawlak Johannes , Cappello Tomas Dal , Schultheiss Georg , Mayer Herbert , Witt Ulrike , Strapazzon Giacomo , Brugger Hermann , Jacob Matthias 
Published: November 2023

AbstractOut-of-hospital reduction of shoulder dislocations using the Campell method is recommended by the International Commission for Alpine Rescue and applied in the Bavarian Mountain Rescue Service (Bergwacht Bayern, BWB) protocols. This prospective observational study includes patients out-of-hospital with suspected shoulder dislocation and treated and evacuated by the BWB. Data were systematically collected using three questionnaires: one completed on-site by the rescuer, the second in hospital by the physician and the third within 28 (8–143) days after the accident by the patient. The suspected diagnosis of shoulder dislocation was confirmed in hospital in 37 (84%) of 44 cases. Concomitant injuries in other body regions were found in eight (16%) of 49 cases and were associated with incorrect diagnosis (p = 0.002). Younger age (p = 0.043) and first shoulder dislocation event (p = 0.038) were associated with a higher success rate for reduction attempts. Out-of-hospital reduction of shoulder dislocations leads to significant pain relief and no poorer long-term outcome. Signs that are associated with successful out-of-hospital reduction (younger age and first event), but also those that are associated with incorrect diagnosis (concomitant injuries) should be considered before trying to reduce shoulder dislocation on site. The considerable rate of incorrect first diagnosis on site should give rise to an intensive discussion around teaching and training for this intervention.Trial registration: This study is registered with the German Registry for Clinical Trials (DRKS00023377).
Humoral immunogenicity assessment after receiving three types of SARS-CoV-2 vaccine
Authors: Najafi Niloofar , Soleimanjahi Hoorieh , Moghaddam-Banaem Lida , Raoufy Mohammad Reza , Shahali Shadab , Kazemnejad Anoshirvan , Nasiri Zeynab 
Published: November 2023

AbstractSeveral vaccines have been developed against SARS-CoV-2 and subsequently approved by national/international regulators. Detecting specific antibodies after vaccination enables us to evaluate the vaccine’s effectiveness. We conducted a prospective longitudinal study among members of Tarbiat Modares University of Tehran, Iran, from 4 September 2021 until 29 December 2021. We aimed to compare the humoral immunogenicity of 3 vaccine types. Participants consisted of 462 adults. Anti-SARS-CoV-2 receptor-binding domain [RBD] IgG titer was compared in 3 groups, each vaccinated by available vaccines in Iran at the time: Oxford/AstraZeneca, COVIran Barekat, and Sinopharm. The median IgG titer was: 91.2, 105.6, 224.0 BAU/ml for Sinopharm, COVIran Barekat and Oxford/AstraZeneca respectively after the first dose; 195.2, 192.0, 337.6 BAU/ml after the second one. We also analyzed the frequency of antibody presence in each vaccine group, in the same order the results were 59.0%, 62.6% and 89.4% after the first dose and 92.1%,89.5% and 98.9% after the second. The comparison of results demonstrated that AstraZeneca vaccine is a superior candidate vaccine for COVID-19 vaccination out of the three. Our data also demonstrated statistically significant higher antibody titer among recipients with an infection history.
Thrombospondin1 antagonist peptide treatment attenuates obesity-associated chronic inflammation and metabolic disorders in a diet-induced obese mouse model
Authors: Zhou Qi , Gwag Taesik , Wang Shuxia 
Published: November 2023

AbstractThrombospondin1 (TSP1) is a multifunctional matricellular protein. Previously, we demonstrated that TSP1 plays a pivotal role in obesity-related inflammation and insulin resistance (IR) by modulating macrophage accumulation and activation in adipose tissue. Moreover, in our in vitro studies, a CD36-derived peptide, functioning as a TSP1 antagonist, effectively inhibited TSP1-induced proinflammatory macrophage activation. However, whether this CD36 peptide can inhibit obesity-induced inflammation and IR in vivo is unknown and determined in this study in a high fat diet-induced obese mouse model (DIO). CD36 peptide or control peptide was intraperitoneally administered into the established obese mice triweekly for 6 weeks. We found that CD36 peptide treatment didn’t affect obesity or weight gain but significantly reduced proinflammatory cytokine production systemically and in visceral fat tissue. Adipose tissue exhibited fewer crown-like structures and reduced macrophage infiltration. CD36 peptide treatment also attenuated the proinflammatory phenotype of bone marrow derived macrophages from obese mice. Furthermore, CD36 peptide treatment improved glucose tolerance and insulin sensitivity, and mitigated obesity-related fatty liver disease and kidney damage. Collectively, this study suggests that the CD36 peptide, as a TSP1 antagonist, shows promise as a novel therapeutic approach for managing obesity-related metabolic disorders.
International survey on complications of religious fasting after metabolic and bariatric surgery
Authors: Kermansaravi Mohammad , Husain Farah A. , Bashir Ahmad , Valizadeh Rohollah , Abbas Syed Imran , Abouzeid Tarek , Amini Masoud , Davarpanah Jazi Amir Hossein , Elfawal Mohamad Hayssam , Gado Waleed , Gee Tikfu , Habeeb Tamer A. A. M. , Al Hadhrami Bader , Inam Atif , Vaziri Nader Moein , Mokhber Somayyeh , Al-momani Hazem , Omerov Taryel , Pazouki Abdolreza , Rezapanah Alireza , Rezvani Masoud , Sadat Mansouri Majid , Sewefy Alaa M. , Taskin Halit Eren , Yunus Tahir , Kassir Radwan , Nimeri Abdelrahman 
Published: November 2023

AbstractReligious fasting in Ramadan the 9th month of the lunar year is one of five pillars in Islam and is practiced for a full month every year. There may be risks with fasting in patients with a history of metabolic/bariatric surgery (MBS). There is little published evidence on the possible complications during fasting and needs stronger recommendations and guidance to minimize them. An international survey was sent to surgeons to study the types of complications occurring during religious fasting in patients with history of MBS to evaluate the risk factors to manage and prepare more evidence-based recommendations. In total, 21 centers from 11 countries participated in this survey and reported a total of 132 patients with complications occurring during religious fasting after MBS. The mean age of patients with complications was 36.65 ± 3.48 years and mean BMI was 43.12 ± 6.86 kg/m2. Mean timing of complication occurring during fasting after MBS was 14.18 months. The most common complications were upper GI (gastrointestinal) symptoms including [gastroesophageal reflux disease (GERD), abdominal pain, and dyspepsia], marginal ulcers and dumping syndrome in 24% (32/132), 8.3% (11/132) and 23% (31/132) patients respectively. Surgical management was necessary in 4.5% of patients presenting with complications (6/132) patients due to perforated marginal or peptic ulcer in Single Anastomosis Duodenoileostomy with Sleeve gastrectomy (SADI-S), one anastomosis gastric bypass (OAGB) and sleeve gastrectomy (SG), obstruction at Jejunojenostomy after Roux-en-Y gastric bypass (RYGB) (1/6) and acute cholecystitis (1/6). Patients after MBS should be advised about the risks while fasting including abdominal pain, dehydration, and peptic ulcer disease exacerbation, and a thorough review of their medications is warranted to minimize complications.
Registration in the supine position improve the accuracy of cup placement in total hip arthroplasty using a portable navigation system
Authors: Naito Yohei , Hasegawa Masahiro , Tone Shine , Wakabayashi Hiroki , Sudo Akihiro 
Published: November 2023

AbstractPortable navigation systems have been developed for use in total hip arthroplasty (THA) in recent years. Although intraoperative registration in the lateral decubitus position or the supine position is need to create the three-dimensional coordinate system, it is not clear which position is appropriate. The purpose of this study was to assess the accuracy of cup placement in primary THA in the lateral decubitus position using an image-free handheld navigation device with registration in the lateral decubitus or the supine position, and clarify which position is appropriate. This retrospective study included 129 consecutive cementless THAs performed using an image-free handheld navigation device in the lateral decubitus position. Registration in the first 68 hips was performed in the lateral decubitus position and the last 61 hips was performed in the supine position. Postoperative cup radiographic inclination and radiographic anteversion were assessed, and the accuracy was compared between the two groups. The mean absolute errors of the postoperative measured inclination and anteversion from the target angles were 3.9° ± 2.2° and 4.8° ± 3.5° in the lateral group and 2.9° ± 2.7° and 3.2° ± 2.7° in the supine group (p < 0.05). The percentage of cups inside Lewinnek’s safe zone was 94% in the lateral group and 95% in the supine group (ns). The mean absolute values of navigation error in inclination and anteversion were 3.1° ± 2.1° and 4.2° ± 2.8° in the lateral group and 2.3° ± 2.0° and 3.1° ± 2.4° in the supine group (p < 0.05 and p < 0.05). Registration in the supine position improved the accuracy of cup insertion compared with the lateral decubitus position in THA using an image-free handheld navigation device in the lateral decubitus position.
Incidental detection of FGFR3 fusion via liquid biopsy leading to earlier diagnosis of urothelial carcinoma
Authors: Huang Quillan , Mitsiades Irene , Dowst Heidi , Zarrin-Khameh Neda , Noor Attiya Batool , Castro Patricia , Scheurer Michael E. , Godoy Guilherme , Mims Martha P. , Mitsiades Nicholas 
Published: November 2023

AbstractThe rising utilization of circulating tumor DNA (ctDNA) assays in Precision Oncology may incidentally detect genetic material from secondary sources. It is important that such findings are recognized and properly leveraged for both diagnosis and monitoring of response to treatment. Here, we report a patient in whom serial cell-free DNA (cfDNA) monitoring for his known prostate adenocarcinoma uncovered the emergence of an unexpected FGFR3-TACC3 gene fusion, a BRCA1 frameshift mutation, and other molecular abnormalities. Due to the rarity of FGFR3 fusions in prostate cancer, a workup for a second primary cancer was performed, leading to the diagnosis of an otherwise-asymptomatic urothelial carcinoma (UC). Once UC-directed treatment was initiated, the presence of these genetic abnormalities in cfDNA allowed for disease monitoring and early detection of resistance, well before radiographic progression. These findings also uncovered opportunities for targeted therapies against FGFR and BRCA1. Overall, this report highlights the multifaceted utility of longitudinal ctDNA monitoring in early cancer diagnosis, disease prognostication, therapeutic target identification, monitoring of treatment response, and early detection of emergence of resistance.
The oncogenic fusion protein TAZ::CAMTA1 promotes genomic instability and senescence through hypertranscription
Authors: Neil Emily , Paredes Roberto , Pooley Oscar , Rubin Brian , Kouskoff Valerie 
Published: November 2023

AbstractTAZ::CAMTA1 is a fusion protein found in over 90% of Epithelioid Hemangioendothelioma (EHE), a rare vascular sarcoma with an unpredictable disease course. To date, how TAZ::CAMTA1 initiates tumour formation remains unexplained. To study the oncogenic mechanism leading to EHE initiation, we developed a model system whereby TAZ::CAMTA1 expression is induced by doxycycline in primary endothelial cells. Using this model, we establish that upon TAZ::CAMTA1 expression endothelial cells rapidly enter a hypertranscription state, triggering considerable DNA damage. As a result, TC-expressing cells become trapped in S phase. Additionally, TAZ::CAMTA1-expressing endothelial cells have impaired homologous recombination, as shown by reduced BRCA1 and RAD51 foci formation. Consequently, the DNA damage remains unrepaired and TAZ::CAMTA1-expressing cells enter senescence. Knockout ofCdkn2a, the most common secondary mutation found in EHE, allows senescence bypass and uncontrolled growth. Together, this provides a mechanistic explanation for the clinical course of EHE and offers novel insight into therapeutic options.
Selectivity matters: selective ROCK2 inhibitor ameliorates established liver fibrosis via targeting inflammation, fibrosis, and metabolism
Authors: Zanin-Zhorov Alexandra , Chen Wei , Moretti Julien , Nyuydzefe Melanie S. , Zhorov Iris , Munshi Rashmi , Ghosh Malavika , Serdjebi Cindy , MacDonald Kelli , Blazar Bruce R. , Palmer Melissa , Waksal Samuel D. 
Published: November 2023

AbstractThe pathogenesis of hepatic fibrosis is driven by dysregulated metabolism precipitated by chronic inflammation. Rho-associated coiled-coil-containing protein kinases (ROCKs) have been implicated in these processes, however the ability of selective ROCK2 inhibition to target simultaneously profibrotic, pro-inflammatory and metabolic pathways remains undocumented. Here we show that therapeutic administration of GV101, a selective ROCK2 inhibitor with more than 1000-fold selectivity over ROCK1, attenuates established liver fibrosis induced by thioacetamide (TAA) in combination with high-fat diet in mice. GV101 treatment significantly reduces collagen levels in liver, associated with downregulation of pCofilin, pSTAT3, pAkt, while pSTAT5 and pAMPK levels are increased in tissues of treated mice. In vitro, GV101 inhibits profibrogenic markers expression in fibroblasts, adipogenesis in primary adipocytes and TLR-induced cytokine secretion in innate immune cells via targeting of Akt-mTOR-S6K signaling axis, further uncovering the ROCK2-specific complex mechanism of action and therapeutic potential of highly selective ROCK2 inhibitors in liver fibrosis.
C-X-C domain ligand 14-mediated stromal cell–macrophage interaction as a therapeutic target for hand dermal fibrosis
Authors: Goto Atsushi , Komura Shingo , Kato Koki , Maki Rie , Hirakawa Akihiro , Tomita Hiroyuki , Hirata Akihiro , Yamada Yasuhiro , Akiyama Haruhiko 
Published: November 2023

AbstractDupuytren’s contracture, a superficial dermal fibrosis, causes flexion contracture of the affected finger, impairing hand function. Specific single-nucleotide polymorphisms within genes in the Wnt signalling pathway are associated with the disease. However, the precise role of Wnt signalling dysregulation in the onset and progression of Dupuytren’s contracture remains unclear. Here, using a fibrosis mouse model and clinical samples of human Dupuytren’s contractures, we demonstrate that the activation of Wnt/β-catenin signalling in Tppp3-positive cells in the dermis of the paw is associated with the development of fibrosis. Fibrosis development and progression via Wnt/β-catenin signalling are closely related to stromal cell–macrophage interactions, and Wnt/β-catenin signalling activation in Tppp3-positive stromal cells causes M2 macrophage infiltration via chemokine Cxcl14, resulting in the formation of a TGF-β-expressing fibrotic niche. Inhibition of Cxcl14 mitigates fibrosis by decreasing macrophage infiltration. These findings suggest that Cxcl14-mediated stromal cell–macrophage interaction is a promising therapeutic target for Wnt/β-catenin-induced fibrosis.
Design and evaluation of tadpole-like conformational antimicrobial peptides
Authors: Tang Ziyi , Jiang Wuqiao , Li Shuangli , Huang Xue , Yang Yi , Chen Xiaorong , Qiu Jingyi , Xiao Chuyu , Xie Ying , Zhang Xu , Li Jianguo , Verma Chandra Shekhar , He Yun , Yang Aimin 
Published: November 2023

AbstractAntimicrobial peptides are promising alternatives to conventional antibiotics. Herein, we report a class of “tadpole-like” peptides consisting of an amphipathic α-helical head and an aromatic tail. A structure-activity relationship (SAR) study of “tadpole-like” temporin-SHf and its analogs revealed that increasing the number of aromatic residues in the tail, introducing Arg to the α-helical head and rearranging the peptide topology dramatically increased antimicrobial activity. Through progressive structural optimization, we obtained two peptides, HT2 and RI-HT2, which exhibited potent antimicrobial activity, no hemolytic activity and cytotoxicity, and no propensity to induce resistance. NMR and molecular dynamics simulations revealed that both peptides indeed adopted “tadpole-like” conformations. Fluorescence experiments and electron microscopy confirmed the membrane targeting mechanisms of the peptides. Our studies not only lead to the discovery of a series of ultrashort peptides with potent broad-spectrum antimicrobial activities, but also provide a new strategy for rational design of novel “tadpole-like” antimicrobial peptides.
Fe protein docking transduces conformational changes to MoFe nitrogenase active site in a nucleotide-dependent manner
Authors: Tokmina-Lukaszewska Monika , Huang Qi , Berry Luke , Kallas Hayden , Peters John W. , Seefeldt Lance C. , Raugei Simone , Bothner Brian 
Published: November 2023

AbstractThe reduction of dinitrogen to ammonia catalyzed by nitrogenase involves a complex series of events, including ATP hydrolysis, electron transfer, and activation of metal clusters for N2 reduction. Early evidence shows that an essential part of the mechanism involves transducing information between the nitrogenase component proteins through conformational dynamics. Here, millisecond time-resolved hydrogen-deuterium exchange mass spectrometry was used to unravel peptide-level protein motion on the time scale of catalysis of Mo-dependent nitrogenase from Azotobacter vinelandii. Normal mode analysis calculations complemented this data, providing insights into the specific signal transduction pathways that relay information across protein interfaces at distances spanning 100 Å. Together, these results show that conformational changes induced by protein docking are rapidly transduced to the active site, suggesting a specific mechanism for activating the metal cofactor in the enzyme active site.
Particle-in-cell simulations of electron–positron cyclotron maser forming pulsar radio zebras
Authors: Labaj Matúš , Benáček Jan , Karlický Marian 
Published: November 2023

Context. The microwave radio dynamic spectra of the Crab pulsar interpulse contain fine structures represented via narrowband quasiharmonic stripes. The pattern significantly constrains any potential emission mechanism. Similar to the zebra patterns observed, for example, in type IV solar radio bursts or decameter and kilometer Jupiter radio emission, the double plasma resonance (DPR) effect of the cyclotron maser instability may allow for interpretion of observations of pulsar radio zebras.
Aims. We provide insight at kinetic microscales of the zebra structures in pulsar radio emissions originating close to or beyond the light cylinder.
Methods. We present electromagnetic relativistic particle-in-cell (PIC) simulations of the electron–positron cyclotron maser for cyclotron frequency smaller than the plasma frequency. In four distinct simulation cycles, we focused on the effects of varying the plasma parameters on the instability growth rate and saturation energy. The physical parameters were the ratio between the plasma and cyclotron frequency, the density ratio of the “hot” loss-cone to the “cold” background plasma, the loss-cone characteristic velocity, and comparison with electron–proton plasma.
Results. In contrast to the results obtained from electron–proton plasma simulations (for example, in solar system plasmas), we find that the pulsar electron–positron maser instability does not generate distinguishable X and Z modes. On the contrary, a singular electromagnetic XZ mode was generated in all studied configurations close to or above the plasma frequency. The highest instability growth rates were obtained for the simulations with integer plasma-to-cyclotron frequency ratios. The instability is most efficient for plasma with characteristic loss-cone velocity in the range vth = 0.2 − 0.3c. For low density ratios, the highest peak of the XZ mode is at double the frequency of the highest peak of the Bernstein modes, indicating that the radio emission is produced by a coalescence of two Bernstein modes with the same frequency and opposite wave numbers. Our estimate of the radiative flux generated from the simulation is up to ∼30 mJy from an area of 100 km2 for an observer at 1 kpc distance without the inclusion of relativistic beaming effects, which may account for multiple orders of magnitude.
A comparative study of two X2.2 and X9.3 solar flares observed with HARPS-N
Authors: Pietrow A. G. M. , Cretignier M. , Druett M. K. , Alvarado-Gómez J. D. , Hofmeister S. J. , Verma M. , Kamlah R. , Baratella M. , Amazo-Gómez E. M. , Kontogiannis I. , Dineva E. , Warmuth A. , Denker C. , Poppenhaeger K. , Andriienko O. , Dumusque X. , Löfdahl M. G. 
Published: November 2023

Context.Stellar flares cannot be spatially resolved, which complicates ascertaining the physical processes behind particular spectral signatures. Due to their proximity to Earth, solar flares can serve as a stepping stone for understanding their stellar counterparts, especially when using a Sun-as-a-star instrument and in combination with spatially resolved observations.Aims.We aim to understand the disk-integrated spectral behaviors of a confined X2.2 flare and its eruptive X9.3 successor, which had energies of 2.2 × 1031erg and 9.3 × 1031erg, respectively, as measured by Sun-as-a-star observations with the High Accuracy Radial velocity Planet Searcher for the Northern hemisphere (HARPS-N).Methods.The behavior of multiple photospheric (NaD1&D2, Mg Iat 5173 Å, Fe Iat 6173 Å, and Mn Iat 4031 Å) and chromospheric (Ca IIH & K, Hα, Hβ, and He ID3) spectral lines were investigated by means of activity indices and contrast profiles. A number of different photospheric lines were also investigated by means of equivalent widths, and radial velocity measures, which were then related to physical processes directly observed in high-resolution observations made with the Swedish 1-m Solar Telescope (SST) and the Atmospheric Imaging Assembly (AIA) on board of the Solar Dynamics Observatory (SDO).Results.Our findings suggest a relationship between the evolving shapes of contrast profile time and the flare locations, which assists in constraining flare locations in disk-integrated observations. In addition, an upward bias was found in flare statistics based on activity indices derived from the Ca IIH & K lines. In this case, much smaller flares cause a similar increase in the activity index as that produced by larger flares. Hα-based activity indices do not show this bias and are therefore less susceptible to activity jitter. Sodium line profiles show a strongly asymmetric response during flare activity, which is best captured with a newly defined asymmetrical sodium activity index. A strong flare response was detected in Mn Iline profiles, which is unexpected and calls for further exploration. Intensity increases in Hα, Hβ, and certain spectral windows of AIA before the flare onset suggest their potential use as short-term flare predictors.