bluuebunny/crossref_metadata_2025_split huggingface dataset:
HUBUNGAN COPING STRESS DENGAN SUBJECTIVE WELL BEING MAHASISWA RANTAU DALAM MENYUSUN TUGAS AKHIR
Authors: Jeman Andriani Flaviani , Ulfa Miftakhul , Rufaindah Ervin 
Published: November 2023

Coping stress yang baik dapat meningkatkan kesejahteraan dan kebahagiaan individu dalam kehidupan sehari-hari. Individu yang mampu mengevaluasi kepuasan hidupnya, dapat juga mengontrol emosinya dalam menghadapi berbagai peristiwa dalam hidup dengan lebih baik. Untuk itu diperlukan coping stress yang baik sehingga dapat meningkatkan kesejahteraan individu. Mengetahui hubungan antara coping stress dengan subjective well-being pada mahasiswa rantau dalam menyusun tugas akhir di STIKES Widyagama Husada Malang Penelitian ini menggunakan analitik korelasional dengan pendekatan cross sectional. Teknik pengumpulan data menggunakan kuesioner yang dilakukan pada 62 responden mahasiswa rantau. Uji statistik menggunakan uji somers. Hasil penelitian ini menunjukkan terdapat hubungan signifikan antara coping stress dengan subjective well being pada mahasiswa rantau (p=0,022). Nilai korelasi dalam penelitian ini menunjukkan bahwa arah korelasi positif dengan kekuatan korelasi yang kuat.
Nanoscaled biphasic calcium phosphate modulates osteogenesis and attenuates LPS-induced inflammation
Authors: Su Yi-Chun , Phan Trinh T. T. , Wang Tzu-Wei , Chang Shao-Hsuan , Lin Feng-Huei , Hsu Tzu-Sheng , Lin Lih-Yuan 
Published: November 2023

Micron-scale structure biphasic calcium phosphate (BCP) materials have demonstrated promising clinical outcomes in the field of bone tissue repair. However, research on biphasic calcium phosphate materials at the nanoscale level remains limited. In this study, we synthesize granular-shaped biphasic calcium phosphate nanomaterials with multiple desirable characteristics, including negatively charged surfaces, non-cytotoxicity, and the capability to penetrate cells, using a nanogrinding dispersion process with a polymeric carboxylic acid as the dispersant. Our results reveal that treating human osteoblasts with 0.5 μg/mL biphasic calcium phosphate nanomaterials results in a marked increase in alkaline phosphatase (ALP) activity and the upregulation of osteogenesis-related genes. Furthermore, these biphasic calcium phosphate nanomaterials exhibit immunomodulatory properties. Treatment of THP-1-derived macrophages with BCP nanomaterials decreases the expression of various inflammatory genes. Biphasic calcium phosphate nanomaterials also mitigate the elevated inflammatory gene expression and protein production triggered by lipopolysaccharide (LPS) exposure in THP-1-derived macrophages. Notably, we observe that biphasic calcium phosphate nanomaterials have the capacity to reverse the detrimental effects of LPS-stimulated macrophage-conditioned medium on osteoblastic activity and mineralization. These findings underscore the potential utility of biphasic calcium phosphate nanomaterials in clinical settings for the repair and regeneration of bone tissue. In conclusion, this study highlights the material properties and positive effects of biphasic calcium phosphate nanomaterials on osteogenesis and immune regulation, opening a promising avenue for further research on inflammatory osteolysis in patients undergoing clinical surgery.
Clinical screening of Nocardia in sputum smears based on neural networks
Authors: Sun Hong , Xie Xuanmeng , Wang Yaqi , Wang Juan , Deng Tongyang 
Published: November 2023

ObjectiveNocardia is clinically rare but highly pathogenic in clinical practice. Due to the lack of Nocardia screening methods, Nocardia is often missed in diagnosis, leading to worsening the condition. Therefore, this paper proposes a Nocardia screening method based on neural networks, aiming at quick Nocardia detection in sputum specimens with low costs and thereby reducing the missed diagnosis rate.MethodsFirstly, sputum specimens were collected from patients who were infected with Nocardia, and a part of the specimens were mixed with new sputum specimens from patients without Nocardia infection to enhance the data diversity. Secondly, the specimens were converted into smears with Gram staining. Images were captured under a microscope and subsequently annotated by experts, creating two datasets. Thirdly, each dataset was divided into three subsets: the training set, the validation set and the test set. The training and validation sets were used for training networks, while the test set was used for evaluating the effeteness of the trained networks. Finally, a neural network model was trained on this dataset, with an image of Gram-stained sputum smear as input, this model determines the presence and locations of Nocardia instances within the image.ResultsAfter training, the detection network was evaluated on two datasets, resulting in classification accuracies of 97.3% and 98.3%, respectively. This network can identify Nocardia instances in about 24 milliseconds per image on a personal computer. The detection metrics of mAP50 on both datasets were 0.780 and 0.841, respectively.ConclusionThe Nocardia screening method can accurately and efficiently determine whether Nocardia exists in the images of Gram-stained sputum smears. Additionally, it can precisely locate the Nocardia instances, assisting doctors in confirming the presence of Nocardia.
Plasmacytoid dendritic cells contribute to vascular endothelial dysfunction in type 2 diabetes
Authors: Alluri K. , Srinivas B. , Belmadani S. , Matrougui K. 
Published: November 2023

ObjectiveType 2 diabetes (T2D) is associated with an increased risk of cardiovascular disease due to macro- and microvascular dysfunction. This study aimed to investigate the potential involvement of plasmacytoid dendritic cells (pDCs) in T2D-related vascular dysfunction.Approach and resultspDCs were isolated from db/db and control mice. It was found that pDCs from db/db mice impaired endothelial cell eNOS phosphorylation in response to ATP and decreased vascular endothelium-dependent relaxation compared to pDCs from control mice. Moreover, isolated CD4+ cells from control mice, when stimulated overnight with high glucose and lipids, and isolated pDCs from db/db mice, display elevated levels of ER stress, inflammation, and apoptosis markers. Flow cytometry revealed that pDC frequency was higher in db/db mice than in controls. In vivo, the reduction of pDCs using anti-PDCA-1 antibodies in male and female db/db mice for 4 weeks significantly improved vascular endothelial function and eNOS phosphorylation.ConclusionpDCs may contribute to vascular dysfunction in T2D by impairing endothelial cell function. Targeting pDCs with anti-PDCA-1 antibodies may represent a promising therapeutic strategy for improving vascular endothelial function in T2D patients. This study provides new insights into the pathogenesis of T2D-related vascular dysfunction and highlights the potential of immunomodulatory therapies for treating this complication. Further studies are warranted to explore the clinical potential of this approach.
Enhancing decellularized vascular scaffolds with PVDF and PCL reinforcement: a fused deposition modeling approach
Authors: Klyshnikov Kirill Yu. , Rezvova Maria A. , Belikov Nikita V. , Glushkova Tatiana V. , Ovcharenko Evgeny A. 
Published: November 2023

BackgroundDecellularized xenogenic scaffolds represent a promising substrate for tissue-engineered vascular prostheses, particularly those with smaller diameters (&lt;6 mm). Despite their benefits, a notable limitation presents itself during decellularization, namely, the diminished mechanical strength that introduces the risk of aneurysmal dilations in the early post-implantation period. This study introduces a strategy for modification the mechanical properties of these biological scaffolds through the forming of an external polymeric reinforcement via thermal extrusion.MethodsThe study utilized scaffolds fabricated from bovine internal mammary arteries through decellularization and preservation. The scaffolds were divided into subgroups and reinforced with polymeric helices made of Polyvinylidene fluoride (PVDF) and Polycaprolactone (PCL), n = 5 for each. An experimental setup for external reinforcement coating was designed. Computed microtomography was employed to obtain accurate 3D models of the scaffolds. Mechanical properties were evaluated through in vitro uniaxial tension tests (Z50, Zwick/Roell, Germany), compliance evaluation and numerical simulations (Abaqus/CAE, Dassault Systemes, France) to investigate the effect of external reinforcement on aneurysm growth.ResultsUsing a double-layer helix for the reinforcement significantly enhanced the radial tensile strength of the scaffolds, increasing it up to 2.26 times. Yet, the comparison of vessel's compliance between two reinforced and the Control scaffolds within the physiological pressures range did not reveal any significant differences. Numerical simulation of aneurysm growth showed that thin-walled regions of the Control scaffold developed aneurysmal-type protrusions, bulging up to 0.7 mm, with a substantial degradation of mechanical properties. In contrast, both PVDF and PCL reinforced scaffolds did not exhibit significant property degradation, with deformations ranging 0.1–0.13 mm depending on the model, and a maximum decrease in the modulus of elasticity of 23%.ConclusionThe results of the study demonstrated that the external polymer helical reinforcement of decellularized scaffolds via thermal extrusion enables a controlled modification of mechanical properties, notably enhancing radial strength while maintaining sufficient compliance within the physiological pressure range. A series of in vitro tests demonstrated the consistency and potential of this approach for decellularized xenogenic scaffolds, a concept that had not been explored before.
Aging exacerbates cardiac dysfunction and mortality in sepsis through enhancing TLR2 activity
Authors: Zhai Yufeng , Yao Qingzhou , The Erlinda , Ao Lihua , Fullerton David A. , Meng Xianzhong 
Published: November 2023

IntroductionSepsis is prevalent in the elderly population with increased incidence and mortality. Currently, the mechanism by which aging increases the susceptibility to sepsis and worsens outcome is unclear. We tested the hypothesis that aging exacerbates cardiac dysfunction in sepsis through a Toll-like receptor 2 (TLR2)-dependent mechanism.MethodsMale young adult (4–6 months) and old (18–20 months) wild type (WT) and TLR2 knockout (KO) mice were subject to moderate sepsis by cecal ligation and puncture. Additional groups of young adult and old WT mice were treated with TLR2 agonist Pam3CSK4. Left ventricle (LV) performance was evaluated with a pressure-volume microcatheter. Tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and monocyte chemoattractant protein-1 (MCP-1) in the myocardium and plasma were assessed using enzyme-linked immunosorbent assay.ResultsSepsis reduced LV ejection fraction and cardiac output in both young adult and old WT mice. However, identical CLP caused more severe cardiac dysfunction and high mortality in old WT mice that were accompanied by greater levels of TNF-α, IL-1β, IL-6 and MCP-1 in the myocardium and plasma. TLR2 KO diminished aging-related difference in myocardial and systemic inflammatory response, resulting in improved cardiac function and decreased mortality in old septic mice. In addition, higher myocardial TLR2 levels in old WT mice resulted in greater myocardial inflammatory response and worse cardiac dysfunction following administration of TLR2 agonist.ConclusionModerate sepsis results in greater cardiac dysfunction and significant mortality in old mice. Aging elevates TLR2 level/activity to exacerbate the inflammatory response to sepsis, leading to worse cardiac dysfunction and mortality.
Family history of malignant or benign thyroid tumors: implications for surgical procedure management and disease-free survival
Authors: Jiang Yu-jia , Han Zhuo-jun , Hu Yi-xuan , Zhang Ning , Huang Tao 
Published: November 2023

BackgroundCurrent guidelines lack a standardized management for patients with family history of thyroid carcinoma (fTC),particularly benign thyroid neoplasm (fBTN). Our objective was to investigate the influence of various family histories on the selection of surgical approaches and disease-free survival (DFS).MethodsA cohort study was conducted involving 2261 patients diagnosed with differentiated thyroid carcinoma including those with fTC (n=224), fBTN (n=122), and individuals without a family history of thyroid carcinoma (nfTC; n=1915). Clinicopathological characteristics were collected. DFS was estimated using Kaplan-Meier analysis and factors affecting DFS were identified using Cox proportional hazard model.ResultsCompared to nfTC, small tumor size, clinically lymph node-positive, extrathyroidal extension, vascular invasion, Hashimoto’s disease and nodular goiter were more common in fTC and fBTN groups. They had lower T stage and a lower rate of good response to TSH suppression therapy but received more radioiodine therapy. It is worth noting that fTC is associated with male, bilateral and multifocal tumors, as well as central lymph node metastasis and distant metastasis. Both fTC (aHR = 2.45, 95% CI=1.11-5.38; P = 0.03) and fBTN (aHR = 3.43, 95% CI=1.27-9.29; P = 0.02) were independent predictors of DFS in patients who underwent lobectomy, but not total thyroidectomy. For 1-4 cm thyroid carcinomas with clinically node-negative, fTC was identified as an independent predictor, whereas fBTN was not.ConclusionOur findings indicate that a family history, particularly of malignancy, is associated with a more aggressive disease. Family history does not affect the prognosis of patients who undergo total thyroidectomy, but it may increase the risk of postoperative malignant events in those who have a lobectomy. Additionally, it may be necessary to monitor individuals with a family history of benign thyroid neoplasms.
Study on low-heat synergistic chemical profile control and flooding technology in Bohai Oilfield
Authors: Dan Liang , Jian Zhang , Wensheng Zhou , Zhao Hua , Qichen Zhang , Liqi Wang 
Published: November 2023

Introduction: Through the combination of hot water and chemical agent, the low-heat synergistic chemical profile control and flooding technology can not only reduce the risks of sand production and string damage caused by high-temperature thermal recovery technology, but also further improve the recovery factor on the basis of hot water flooding.Methods: Based on laboratory testing, the synergism mechanism of thermal energy and chemical agent is studied.Results: The research results showed that in the near wellbore zone of the injection well, crude oil viscosity reduction mainly relies on thermal energy, and chemical agents assist in improving the water oil mobility ratio and reducing the risk of cross flow. After entering the deep formation, due to the decrease in temperature, the thermal energy effect is weakened, but the chemical agents still aggregate at the oil-water interface and continue to act on heavy oil to reduce viscosity. The higher the temperature, the greater the role of heat energy, and the weaker the role of chemical agents. The higher the injection concentration of chemical agent, the more obvious the leading role of chemistry. Based on the comprehensive evaluation index, the optimized temperature is 80°C and the injection concentration is 1500 mg/L. The scheme study of Bohai L Oilfield shows that compared with cold water flooding, hot water flooding can only improve oil recovery by 1.2 percentage points, and low heat collaborative chemical profile control flooding technology can further improve oil recovery by 4.7 percentage points on the basis of hot water flooding, with obvious oil increase effect.Conclusion: It is suggested that Bohai L Oilfield should apply low-heat coordinated chemical profile control and flooding technology to improve the development effect of the oilfield, and provide new technical ideas for safe and economic development of offshore heavy oil.
Assessing the structure and diversity of fungal community in plant soil under different climatic and vegetation conditions
Authors: Liu Sen , Xiong Chengjie , Lin Longbing , Keyhani Nemat O. , Zhu Mengjia , Zhao Zhiying , Zhang Weibin , Yang Chenjie , Su Hailan , Liu Pu , Guan Xiayu , Qiu Junzhi 
Published: November 2023

IntroductionUnderstanding microbial communities in diverse ecosystems is crucial for unraveling the intricate relationships among microorganisms, their environment, and ecosystem processes. In this study, we investigated differences in the fungal community structure and diversity in soils from two contrasting climatic and vegetation conditions: the Xinjiang western China plateau and the Fujian southeastern coastal province.MethodsA total of 36 soil samples collected from two climatic regions were subjected to high-throughput ITS gene sequencing for fungal community analysis. In conjunction soil physicochemical properties were assessed and compared. Analyses included an examination of the relationship of fungal community structure to environmental factors and functional profiling of the community structure was using the FUNGuild pipeline.ResultsOur data revealed rich fungal diversity, with a total of 11 fungal phyla, 31 classes, 86 orders, 200 families, 388 genera, and 515 species identified in the soil samples. Distinct variations in the physicochemical properties of the soil and fungal community structure were seen in relation to climate and surface vegetation. Notably, despite a colder climate, the rhizosphere soil of Xinjiang exhibited higher fungal (α-)diversity compared to the rhizosphere soil of Fujian. β-diversity analyses indicated that soil heterogeneity and differences in fungal community structure were primarily influenced by spatial distance limitations and vegetation type. Furthermore, we identified dominant fungal phyla with significant roles in energy cycling and organic matter degradation, including members of the Sordariomycetes, Leotiomycetes, Archaeosporomycetes, and Agaricomycetes. Functional analyses of soil fungal communities highlighted distinct microbial ecological functions in Xinjiang and Fujian soils. Xinjiang soil was characterized by a focus on wood and plant saprotrophy, and endophytes, whereas in Fujian soil the fungal community was mainly associated with ectomycorrhizal interactions, fungal parasitism, and wood saprotrophy.DiscussionOur findings suggest fungal communities in different climatic conditions adapt along distinct patterns with, plants to cope with environmental stress and contribute significantly to energy metabolism and material cycling within soil-plant systems. This study provides valuable insights into the ecological diversity of fungal communities driven by geological and environmental factors.
A risk signature based on endoplasmic reticulum stress-associated genes predicts prognosis and immunity in pancreatic cancer
Authors: Chen Haofei , Xu Ning , Xu Jia , Zhang Cheng , Li Xin , Xu Hao , Zhu Weixiong , Li Jinze , Liang Daoming , Zhou Wence 
Published: November 2023

Introduction: The involvement of endoplasmic reticulum (ER) stress in cancer biology is increasingly recognized, yet its role in pancreatic cancer (PC) remains unclear. This study aims to elucidate the impact of ER stress on prognosis and biological characteristics in PC patients.Methods: A bioinformatic analysis was conducted using RNA-seq data and clinicopathological information from PC patients in the TCGA and ICGC databases. The ER stress-associated gene sets were extracted from MSigDB. ER stress-associated genes closely linked with overall survival (OS) of PC patients were identified via log-rank test and univariate Cox analysis, and further narrowed by LASSO method. A risk signature associated with ER stress was formulated using multivariate Cox regression and assessed through Kaplan-Meier curves, receiver operating characteristic (ROC) analyses, and Harrell’s concordance index. External validation was performed with the ICGC cohort. The single-sample gene-set enrichment analysis (ssGSEA) algorithm appraised the immune cell infiltration landscape.Results: Worse OS in PC patients with high-risk signature score was observed. Multivariate analysis underscored our ER stress-associated signature as a valuable and independent predictor of prognosis. Importantly, these results based on TCGA were further validated in ICGC dataset. In addition, our risk signature was closely associated with homeostasis, protein secretion, and immune regulation in PC patients. In particular, PC microenvironment in the high-risk cluster exhibited a more immunosuppressive status. At last, we established a nomogram model by incorporating the risk signature and clinicopathological parameters, which behaves better in predicting prognosis of PC patients.Discussion: This comprehensive molecular analysis presents a new predictive model for the prognosis of PC patients, highlighting ER stress as a potential therapeutic target. Besides, the findings indicate that ER stress can have effect modulating PC immune responses.
Clinical Functional Seizure Score (CFSS): a simple algorithm for clinicians to suspect functional seizures
Authors: Dashtkoohi Mohammad , Ranji-Bourachaloo Sakineh , Pouremamali Rozhina , Dashtkoohi Mohadese , Zamani Raha , Moeinafshar Aysan , Shizarpour Arshia , Shakiba Shirin , Babaee Mohammadali , Tafakhori Abbas 
Published: November 2023

PurposeDistinguishing functional seizures (FS) from epileptic seizures (ES) poses a challenge due to similar clinical manifestations. The creation of a clinical scoring system that assists in accurately diagnosing patients with FS would be a valuable contribution to medical practice. This score has the potential to enhance clinical decision-making and facilitate prompt diagnosis of patients with FS.MethodsParticipants who met the inclusion criteria were randomly divided into three distinct groups: training, validation, and test cohorts. Demographic and semiological variables were analyzed in the training cohort by univariate analyses. Variables that showed a significant difference between FS and ES were then further scrutinized in two multivariate logistic regression models. The CFSS was developed based on the odds ratio of the discriminating variables. Using the validation group, the optimal cutoff value was determined based on the AUC, and then the CFSS was evaluated in the test cohort to assess its performance.ResultsThe developed score yielded an AUC of 0.78 in the validation cohort, and a cutoff point of 6 was established with a focus on maximizing sensitivity without significantly compromising specificity. The score was then applied in the test cohort, where it achieved a sensitivity of 86.96% and a specificity of 73.81%.ConclusionWe have developed a new tool that shows promising results in identifying patients suspicious of FS. With further analysis through prospective studies, this innovative, simple tool can be integrated into the diagnostic process of FS.