bluuebunny/crossref_metadata_2025_split huggingface dataset:
TIPE2 protein negatively mediates restoration of myeloid dendritic cells in patients with chronic hepatitis B
Authors: Ma Li , Kang Yanhong , Wei Junfeng , Qin Xinmeng , Wei Xun , Kang Yuehua , Kang Yi 
Published: October 2023

Abstract
Background
Hepatitis B virus (HBV) causes persistence in a subgroup of patients and impaired myeloid dendritic cells (mDCs) functions have been observed in these patients, and the effect could be remedied by treatment with interferon-α (IFN-α)-based antiviral therapies. However, the biological functions of mDCs in HBV infection remains largely unexplored.
Methods
TIPE2 expression in mDCs was analyzed by qRT-PCR, western blot and flow cytometry. The release of cytokines was assessed using enzyme-linked immunosorbent assay (ELISA). Downregulation of TIPE2 expression was constructed via siRNA.
Results
Our results showed that TIPE2 was significantly increased in mDCs isolated from chronically HBV-infected subjects compared with healthy subjects or patients achieving antiviral treatment of sustained virological responses (SVR). Interestingly, IFN-α treatment could decrease the expression of TIPE2 in mDCs from HBV patients than that untreated patients, SVR patients, or healthy subjects. Moreover, TIPE2 expression in mDCs was decreased in healthy subjects but not HBV patients after stimulating with poly I:C, while the difference could be abrogated by the treatment with IFN-α in vitro. In addition, TIPE2 expression by poly I:C activated mDCs, with or without IFN-α treatment, negatively correlated with the expression of CD86 and CCR7, and the secretion of interleukin-12 (IL-12), but positively associated with the production of IL-10. Finally, downregulation expression of TIPE2 could increase the production of IL-12 and decrease IL-10 secretion in mDCs of chronically HBV-infected individuals.
Conclusions
Our study suggested that TIPE2 was a crucial factor in negatively mediating innate immune responses during chronic viral infection.
Abdominal vascular injury caused by lumbar reoperation surgery：a case report
Authors: Yang Jia , Cui Zhong-Ning , Dong Jia-Nan , Zheng Jia-Le , Pang Li-Kai 
Published: October 2023

Abstract
Purpose
We report a case of pedicle screw injury to the abdominal inferior vena cava （IVC）during lumbar reoperation.
Methods
A 67-year-old man was hospitalized again for low back pain and numbness in his lower limbs. The imaging results of the patient's re-hospitalization showed that the L4/5 intervertebral space was not fused, and the L5 bilateral pedicle screw was broken. When we removed the fractured pedicle screw on the left side, the screw was accidentally pushed to the abdomen. Fortunately, the patient 's blood pressure did not show a progressive decrease.
Results
We decisively made open exploration of the left approach to him, and successfully removed the pedicle screw. At the same time, we made bone graft and internal fixation of the L4/5 intervertebral space, and the patients had satisfactory recovery during and after the operation.
Conclusion
Abdominal vascular injury is a rare but fatal complication of posterior lumbar surgery. Lumbar revision surgery is complex, and surgeons must consider it carefully before surgery. Oblique Lumbar Interbody Fusion（OLIF）surgery can meet both lumbar revision and abdominal vascular exploration
Analysis of the application value of speckle-tracking imaging technology to assess fetal cardiac function in the early second trimester
Authors: Luo Ling , Liu Hanmin , Chen Jiao , Dai Xiaohui , Liu Dan , Wu Ting , Zhu Qi 
Published: October 2023

Abstract
Objective
We explored the usefulness of speckle-tracking imaging (STI) to assess cardiac function in fetal congenital heart disease in the early second trimester.
Method
A total of 151 pregnant women with a singleton pregnancy who underwent early fetal echocardiography in our hospital were randomly selected. There were 39 fetuses with congenital heart disease and no significant cardiac configuration change was visualized in the four-chamber view in all cases (case group) and 112 healthy fetuses (control group). STI was performed in both groups to measure fetal left ventricular (LV) and right ventricular (RV) endocardial-myocardial global longitudinal strain (GLSendo), time to peak longitudinal strain (TTPS), fractional area change (FAC), and LV ejection fraction (LVEF). Parameters were compared between the two groups, and correlation analyses were performed.
Results
LVEF, LV FAC, and RV FAC did not significantly differ between groups. The LV GLSendo and RV GLSendo were significantly lower in the case group (P < 0.05). The LV TTPS and RV TTPS were negatively correlated with fetal heart rate in each group. After heart rate correction, the TTPS was significantly longer in the case group.
Conclusion
STI may be more sensitive than conventional imaging for detection of fetal myocardial deformation and function in the early second trimester.
Clinical and radiographic outcome of tension band suture fixation for displaced olecranon fractures
Authors: Vesterby Liv , Ohrt-Nissen Søren , Ban Ilija , Thomsen Morten Grove , Tengberg Peter Toft 
Published: October 2023

Abstract

Background:
Tension band wire fixation (TBW) is a well-described method for treating displaced olecranon fractures. Further surgery is often needed due to wound breakdown or prominent hardware.
An all-suture technique has recently been described as an alternative to TBW but radiographic and clinical outcome are not well established. The aim of this single-center retrospective cohort study was to evaluate outcome after treatment with all-suture technique for simple displaced olecranon fractures.
Methods
A retrospective review of olecranon fractures in patients (>18 years) treated for displaced olecranon fractures with tension band suture fixation (TBSF) between February and August 2019 was performed in our facility. Primary outcome was revision surgery, which was assessed four years after surgery. Clinical and radiographical follow-up was performed at two weeks, six weeks, three months and six months to assess union rate, fracture displacement, range of motion (ROM), Quick-DASH and Oxford Elbow Score.
Results
A total of 24 patients were included. Median age was 64 years [IQR:39-73], 9 patients were male and median ASA score was 2 [IQR:1-2]. 15 fractures were Mayo type 2A and 9 type 2B with minor comminution. At four-year follow-up, three patients had died. None of the remaining 21 patients had undergone revision surgery. At six months, the median Quick-DASH and Oxford Elbow Score were 2.3 [IQR:0-4.5] and 47 [IQR:46-48], respectively. Median elbow extension and flexion deficits were 0° [IQR:0-2.25] and 0° [IQR:0-0], respectively. Radiographic union was achieved in all patients. In two cases radiographic loss of reduction and malunion was observed but both patients were asymptomatic and had no functional deficits. One patient refractured the elbow due to a second trauma and was reoperated.
Conclusions
TBSF is a promising technique for Mayo type 2A and 2B fractures with minor comminution. There were no revision surgeries within the first four years. We found good functional outcomes and a high union rate.
Dynamic testing based versus restrictive based fluid administration following early fluid resuscitation in septic shock patients, a pilot study
Authors: Abdelbaky Shaimaa , Ahmed Walid , Toema Khaled , Elamir Nashwa 
Published: October 2023

Abstract
Background
Efforts have been targeting early reversal of a hemodynamically unstable patient in septic shock. The classic rationale for fluid resuscitation in septic shock is to restore intravascular volume deficit with resultant augmentation of cardiac output. Latest surviving sepsis campaign guidelines recommended an initial fluid bolus with subsequent fluid administration, guided by dynamic testing.
AIM OF WORK
We aimed to test if restrictive fluid protocol was comparable to dynamic-based testing for fluid responsiveness protocol.
METHODS
This was a prospective cohort study, recruiting septic shock patients, conducted in Critical Care Department, Cairo University. All patients were initially resuscitated. Patients were randomized (1:1 ratio) to restrictive group (fluid administration with predetermined rate of 1 ml/kg/hour), and dynamic-based testing group (fluid administration according to dynamic measures, following passive leg raising method). Positive response was considered when cardiac output increased by 10%.
Results
Our study recruited 80 patients (39 males) with age of 57.3±18.8 years old. It was noted that clinical severity scores were higher in the restrictive group, with no significant difference in 3 hours lactate. Fluid strategy did not affect mortality. Logistic regression showed no significant difference regarding adjusted mortality, between those who showed acceptable fluid response according to dynamic testing and those who received a restrictive protocol (Hazard’s risk 0.913, P 0.903, CI 95% 0.212 – 3.935). Fluid strategy did not affect need for dialysis, nor mechanical ventilation, nor length of ICU stay.
CONCLUSION
Restrictive fluid strategy, following initial fluid resuscitation was comparable to dynamic testing-based strategy, in terms of mortality, need for ventilation, need for dialysis and ICU length of stay
Alleviation effects of dexmedetomidine on myocardial ischemia/reperfusion injury through fatty acid metabolism pathway via Elovl6
Authors: Yuan Haozheng , Guo Jingying , Wang Congxia , Zhang Chunyan 
Published: October 2023

Abstract

Dexmedetomidine (Dex) is widely used in the sedation in intensive care units and as an anesthetic adjunct. We applied in vivo rat model as well as in vitro cardiomyocyte models (H9c2 cells and neonatal rat cardiomyocytes, NRCMs) to evaluate the effects of Dex against myocardial I/R injury. Next, transcriptomic sequencing was performed to determine the global change of gene expression and identified genes related to fatty acid metabolism were significantly regulated by Dex. Furthermore, shRNAs targeting Elovl6 were transfected into H9c2 cells and NRCMs to knockdown Elovl6. By comparing the effects of Dex on both wild type and Elovl6-knockdown cell under oxygen-glucose deprivation/reoxygenation (OGD/R) challenge, the protection efficiency of Dex was lower supported by the dataset including the cytotoxicity endpoints (cell viability and LDH release) and apoptosis as well as key gene expressions. These results indicate that Dex exhibited the protective function against myocardial I/R injury via fatty acid metabolism pathways and Elovl6 plays the key role in the process, which was further confirmed using palmitate exposure in both cells. Overall, this study systematically evaluates the protective effects of Dex on the myocardial I/R injury and provide better understanding of the role of fatty acid metabolism in the function of Dex.
Study on the degradation mechanism of cathode in electrochemical water descaling
Authors: Lin Wei , Gao Ze yu , wang wei , Wang Cheng gang , Xiang Jin , Xu Jian min , Yu Jiu yang 
Published: October 2023

Abstract
This article studies the degradation mechanism of cathode descaling efficiency in the electrochemical water descaling process. The prolonged reaction causes many calcium carbonate crystals to adhere to the surface of the electrode plate. It results in significant degradation of the descaling efficiency and eventually approaches 0. This paper focuses on the interactive effects of multiple factors on the degradation of electrochemical water softening descaling efficiency. The results show that when the DC voltage exceeds 20V, the degradation process of descaling efficiency of the electrode plate is prolonged and self-cleaning occurs. The hardness removal amount is increased by 20%. Supported by the LSI and RSI indices, voltage current density and mesh size directly affect the degradation of descaling efficiency. The main reason for the increase of unit energy consumption is the thicker scale, which leads to the decrease of scale removal efficiency between cathode and anode.Combined energy consumption per unit and degradation of descaling efficiency, using a mesh size of 1 mm×3 mm, can reduce the unit energy consumption by 18.75%, effectively prolonging the degradation of descaling efficiency.
Electrochemical noise analysis of corrosion sensitivity of Pb- free solders in 5 wt.% citric acid solution
Authors: Li Liang , Ma Rongyao , Wang Xaojing , Cai ShanShan , Qiao Yanxin , Cao Fengting , Zhang Jiaheng , Chen Cai 
Published: October 2023

Abstract
The corrosion behavior of pure Sn, Sn2Ag, Sn42In and Sn58Bi alloys in 5 wt.% citric acid solutions has been investigated using electrochemical noise analysis combined with potentiodynamic polarization, electrochemical impedance spectroscopy tests and etching morphology observation. The results indicated that Sn58Bi has the lowest corrosion rate, followed by Sn2Ag, Sn42In, and Sn. The corrosion evolution of the four alloys mainly consists of two stages as follow: for pure Sn, the first stage is local corrosion (corrosion pits growth) stage and the second stage is uniform corrosion with preferential dissolution of some grains on the sample surface. In contrast, for dual phase alloys, Sn2Ag, Sn42In, and Sn58Bi alloys, all preferentially underwent selective phase corrosion in the first stage, followed by the growth of uniform corrosion and selective phase corrosion in the second stage. The corrosion incubation rates for the four Sn-based alloys: Sn42In > Sn > Sn2Ag > Sn58Bi for both uniform corrosion and local corrosion. While the probability of corrosion growth in sequence was: Sn > Sn58Bi > Sn42In > Sn2Ag (uniform corrosion), Sn58Bi > Sn > Sn42In > Sn2Ag (local corrosion). In this work, the corrosion behaviors were consistent with the observed corrosion morphology, which provided guidance for understanding the interaction between solder alloys surfaces and acid in flux and further selecting organic acid activators compatible with new solder alloys.
Systematic druggable genome-wide Mendelian randomization identifies novel therapeutic targets or repurposing opportunities for rheumatoid arthritis
Authors: Cai Yongsong , Wang Xinyi , Yuan Qiling , Xu Peng 
Published: October 2023

Abstract

Background Rheumatoid arthritis (RA) is a common autoimmune inflammatory disease. Currently, a complete cure for RA is still unavailable. Mendelian randomization (MR) has emerged as a valuable tool for identifying potential therapeutic targets or drug repurposing opportunities for certain diseases. Therefore, our aim was to identify novel effective targets or drug repurposing opportunities for RA and analyze their mechanisms and potential side effects.
Methods A MR integrating the identified druggable genes was used to evaluate the causal effects of druggable gene cis-expression quantitative trait loci (cis-eQTLs) on RA, while additional RA cohort was employed for validation. Colocalization analysis was performed to determine the probability of shared causal variants between the identified targets and RA. The protein-protein interaction network analysis was conducted to explore associations between the identified druggable genes and current RA drug targets. The MR and colocalization analyses were used to assess the potential side effects of the identified targets in RA treatment.
Results Nine druggable genes (TYK2, PTPN22, ATP2A1, APOM, RXRB, NOTCH4, HLA-DRA, CCR6, and CTLA4) showed significant MR results in both the training cohort (p<1.99E-05) and validation cohort (p<0.0025). Colocalization analysis indicated that cytotoxic T-lymphocyte-associated protein 4 (CTLA4) and RA (PP.H4.abf=0.98), as well as C-C motif chemokine receptor 6 (CCR6) and RA (PP.H4.abf=0.99), shared the same causal variant. Hence, these two genes were identified as the final therapeutic targets. Furthermore, CTLA4 and CCR6 interacted with the current RA drug targets. Subsequent MR analysis revealed that genetically proxied activation of CTLA4 and inhibition of CCR6 might decrease the risk of hypothyroidism but increase the risk of malignant skin neoplasm. Additionally, genetically proxied activation of CTLA4 may also reduce the risk of type 1 diabetes.
Conclusions This study supports the idea that targeting the activation of CTLA4 and the inhibition of CCR6 may reduce the risk of RA with fewer side effects, and highlights the potential of CTLA4 and CCR6 as promising druggable targets for RA treatment.
Antioxidant Activity of Flavonoid Compounds in Lime (Citrus aurantifolia) via Density Functional Theory
Authors: Ahmad Faozan , Kartono Agus , Sukmawati Sisqina Ummi , Sumaryada Tony , Handayasari Faridah , Hardhienata Hendradi 
Published: October 2023

Abstract
The advance of molecular quantum biophysics has opened the path towards a deeper understanding of the microscopic mechanism behind free radicals activity that are responsible for cell damages and oxidative stress. In this work, we perform an \textit{ab-initio} analysis on the antioxidant activity of three flavonoid compounds e.g. hesperetin, quercetin, and naringenin in lime (\textit{Citrus aurantifolia}). We study the antioxidant capabilities of these compounds to understand the quantum mechanical mechanism in combating free radicals in the framework of Density-Functional Theory (DFT) using B3LYP exchange-correlation and def2/SVP basis sets. We found via vibrational frequency calculations, that the antioxidant behaviors of these molecules predominantly arise from hydrogen atom transfer, single electron transfer followed by proton transfer, and sequential proton loss electron transfer mechanisms. Our calculations also show that quercetin showcased superior antioxidant activity compared to hesperetin and naringenin based on their bond dissociation energy, ionization potential + proton dissociation enthalpy, and proton affinity + electron transfer enthalpy values at the C9-OH group, with values of 295.29 kJ/mol, 81.88 kJ/mol, and 81.88 kJ/mol for quercetin respectively. An in-depth look into the antioxidant properties, using global reactivity parameters also affirmed quercetin's preeminence in antioxidant characteristics over hesperetin and naringenin, excelling in nearly all considered parameters, though hesperetin's chemical potential stood out at -3.51 eV.
Regional reef fish assemblage maps provide baseline biogeography for tropicalization monitoring.
Authors: Walker Brian K , Becker Dana Fisco , Williams Gareth J , Kilfoyle Audie K , Smith Steven G. 
Published: October 2023

Abstract
Climate change, overfishing, and pollution have driven changes in the composition of many extant reef fish assemblages. As global temperatures rise, historically temperate locales are becoming inhabited by vagrant tropical species as their ranges expand poleward in a process known as tropicalization. Severe reef fish biomass declines are predicted by 2100 under high ocean warming scenarios. This redistribution of species has serious consequences for economic development, livelihoods, food security, human health and culture. Determining the tropicalization of Western Atlantic reef fish is challenging because ranges are expansive, their distributions temporally shift with the movement of isotherms, and many dynamic density-dependent factors affect occurrence and density. Since fish assemblages are known to vary with depth, habitat type, topography, and regionality and local benthic habitat map classifications include these descriptors in their scheme, we used benthic habitat maps to spatially represent the zoogeography of regional reef fish assemblages. The outcomes illustrate that the southeast Florida coast is a pivotal location in the transition between tropical and temperate marine fauna for North America driven by geology and Florida Current upwelling dynamics. The southern assemblages had a higher occurrence and density of tropical species, whereas the northern assemblages had a higher occurrence and density of temperate species. Delineating the spatial distribution of reef fish assemblages facilitates monitoring and detection of future poleward tropicalization, as will be evident in temporal changes in percent occurrence, assemblage relative abundance, and the homogenization of assemblage regions. These delineations contextualize restoration expectations and success by providing a spatial reference to focus conservation actions based on expectations from natural environmental variability, especially in transitional areas between ecoregions.
The association between 22 psychological cognitive factors and temporomandibular joint disorders:a Mendelian randomization study
Authors: Wang Yuheng , Li Changlei , Liu Fayu , Sun Fujing 
Published: October 2023

Abstract

Background
Although existing research suggests a broad association between psychological cognitive factors and TMD, the specific impact of psychiatric factors on TMD remains unknown, as does the causal relationship between them.
Methods
Utilizing summary statistics obtained from genome-wide association studies predominantly conducted on individuals of European ancestry, we employed a two-sample MR approach to estimate the causal and reverse causal effects of 22 psychological cognitive factors on TMD. Subsequently, for statistically significant findings, we performed multivariable MR analysis and bidirectional MR analysis after categorization to ascertain independent associations and explore potential reverse causation.
Results
We found that intelligence(OR: 0.731, 95%CI: 0.557–0.960), education(OR: 0.487, 95%CI: 0.368–0.644) and cognition(OR: 0.760, 95%CI: 0.586–0.986) were protective causal factors for TMD, with education(OR: 0.501, 95%CI: 0.307–0.818) having the independent association. And we found that major depression(OR: 2.116, 95%CI: 1.542–2.904), anxious feelings(OR: 6.014, 95%CI: 1.692–21.373), nervous feelings(OR: 14.019, 95%CI: 2.499–78.64), depressed feelings(OR: 20.905, 95%CI: 1.695–263.5), sensitivity(OR: 5.965, 95%CI: 1.471–24.19) and neuroticism (OR: 1.258, 95%CI: 1.101–1.438) were the factors that constituted harmful causal effects on TMD, with major depression(OR: 1.964, 95%CI: 1.320–2.920) having the independent relationship. These results were all robust to sensitivity analyses. We found no evidence on causal effects between other phychological cognitive factors and TMD, in either direction.
Conclusions
Our study demonstrated that intelligence, education, and cognition exert a protective influence on TMD, with education exhibiting an independent effect. Furthermore, we elucidated that major depression, anxious feelings, nervous feelings, depressed feelings, sensitivity, and neuroticism pose as risk factors for TMD development, with major depression being an independent predictor. These findings provide valuable insights into the causal relationship between 22 psychological cognitive factors and TMD.