bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploration of Chemical Diversity in Intercellular Quorum Sensing Signalling Systems in Prokaryotes
Authors: Jonkergouw Christopher , Savola Pihla , Osmekhina Ekaterina , van Strien Joeri , Batys Piotr , Linder Markus B. 
Published: October 2023

AbstractQuorum sensing (QS) serves as a vital means of intercellular signalling in a variety of prokaryotes, which enables single cells to act in multicellular configurations. The potential to control community‐wide responses has also sparked numerous recent biotechnological innovations. However, our capacity to utilize intercellular communication is hindered due to a scarcity of complementary signalling systems and a restricted comprehension of interconnections between these systems caused by variations in their dynamic range. In this study, we utilize uniform manifold approximation and projection and extended‐connectivity fingerprints to explore the available chemical space of QS signalling molecules. We investigate and experimentally characterize a set of closely related QS signalling ligands, consisting of N‐acyl homoserine lactones and the aryl homoserine lactone p‐coumaroyl, as well as a set of more widely diverging QS ligands, consisting of photopyrones, dialkylresorcinols, 3,5‐dimethylpyrazin‐2‐ol and autoinducer‐2, and define their performance. We report on a set of six signal‐ and promoter‐orthogonal intercellular QS signalling systems, significantly expanding the toolkit for engineering community‐wide behaviour. Furthermore, we demonstrate that ligand diversity can serve as a statistically significant tool to predict much more complicated ligand‐receptor interactions. This approach highlights the potential of dimensionality reduction to explore chemical diversity in microbial dynamics.
An online sequential training package to teach citation formatting: Within and across participant analyses
Authors: Guinness Kendra E. , Parry‐Cruwys Diana , Atkinson Ryan S. , MacDonald Jacquelyn M. 
Published: October 2023

AbstractWhen teaching complex skills in higher education settings, different individuals may require different levels of instruction to achieve mastery. The current study replicated and extended Parry‐Cruwys et al. (2022) by evaluating the effects of an online sequential training package on accuracy of APA citations with graduate students in behavior analysis. The intervention consisted of (a) a checklist of APA citation criteria, (b) online modules based on principles of behavioral instruction, and (c) email feedback. All components were delivered remotely through course management software and introduced sequentially such that participants experienced only the intervention necessary to meet the mastery criterion. Of 13 participants, seven required the checklist only, two required the checklist and online modules, and four required the checklist, modules, and feedback. An across participant acquisition analysis revealed additional response patterns that could inform the refinement of future training materials. This evaluation demonstrates an efficient mechanism for assessing skill acquisition at the level of the individual learner in the context of higher education.
Implantation depth of balloon‐expandable transcatheter heart valves and risks for permanent pacemaker implantation and midterm adverse events
Authors: Pellegrini Costanza , Freißmuth Markus , Rheude Tobias , Graas David , Mayr N. Patrick , Syryca Finn , Alvarez‐Covarrubias Hector A. , Fetcu Andrei , Hübner Judith , Lennerz Carsten , Schunkert Heribert , Kastrati Adnan , Xhepa Erion , Joner Michael 
Published: October 2023

AbstractBackgroundPermanent pacemaker implantation (PPI) remains a relevant complication after transcatheter aortic valve implantation (TAVI) and its impact on outcome remains controversial.AimsThis study aimed to analyze the effects of implantation depth on PPI at 30 days and assess its impact on outcome with the balloon‐expandable Sapien 3 (S3) prosthesis.MethodsBetween 2014 and 2018, 849 patients without previous pacemaker undergoing transfemoral TAVI with the S3 were included. Prosthesis implantation depth was measured and divided into Quintiles. An ordinal logistic regression was used to assess its association with PPI, while a multivariate logistic regression was performed to identify predictors of PPI. Survival analyses were performed with the Kaplan−Meier method and a multivariable Cox regression was performed to ascertain the impact of PPI on mortality.ResultsOverall, incidence of PPI at 30 days was 9.7%. Implantation depth decreased consistently from a median of 6.7 mm [5.55−8.00] in 2014 to 2.7 mm [2.30−3.50] in 2018 (p < 0.001). When considering Quintiles of implantation depth, incidence of PPI was significantly higher in upper Quintiles and risk for PPI was significantly lower for the 1. Quintile compared to the 5. Quintile (OR: 0.34, 95% CI: [0.16−0.73]; p = 0.003). In the adjusted multivariable logistic regression implantation depth persisted ad independent predictor of PPI at 30 days. Patients requiring PPI at 30 days displayed significantly higher mortality at 4 years compared to patients without PPI (49.5% vs. 40.0%; log‐rank = 0.022). In a multivariate analysis, increased logistic EuroScore, diabetes mellitus, and history of atrial fibrillation, were independent predictors of all‐cause mortality at 2 years.ConclusionsHigher prosthesis implantation relative to the virtual aortic annulus was significantly associated with reduced risk for PPI at 30 days. Patients with PPI at 30 days exhibited higher mortality during follow‐up, however, only logistic EuroScore, diabetes mellitus, and history of atrial fibrillation were identified as independent predictors of mortality at 2 years.
Pressure‐Assisted Synthesis of Highly Crystalline 1T′′‐LixMoS2
Authors: Schwarzmüller Stefan , Wurst Klaus , Heymann Gunter , Huppertz Hubert 
Published: October 2023

AbstractLixMoS2 is not only a lithium battery material, but is also an important precursor for the synthesis of MoS2 nanomaterials. Current syntheses of MoS2, such as in n‐butyllithium/LiBH4 or electrochemically, are not satisfying in terms of defined stoichiometry and crystallinity, so an accurate experimental crystal structure determination of this important and widely used material has been long awaited. A high‐pressure/high‐temperature synthesis yielded highly crystalline 1T′′‐LixMoS2 (x=1, 1.333). 1T′′‐LiMoS2 crystallizes in the space group P
with a=6.2482(3) Å, b=6.6336(3) Å, c=6.7480(3) Å, α=119.321(2)°, β=90.010(2)° and γ=90.077(2)°. The arrangement of Mo atoms within the b‐c‐plane confirmed a predicted Peierls distortion. A similar atom distribution pattern to that of Mo is also observed for the lithium atoms. Calculation of bond valence site energies gave an activation barrier of 1.244 eV for 2D lithium‐ion migration. For x=1.333, a phase‐pure synthesis was achieved.
Cryoballoon ablation for atrial fibrillation in patients with heart failure and reduced left ventricular ejection fraction: A systematic review and meta‐analysis
Authors: Taha Amira M. , Hendi Nada I. , Elwekel Ahmed B. , Atia Ahmed , Taha Nouran A. , Shrestha Abhigan B. , Elbanna Mohamed 
Published: October 2023

AbstractThe coexistence of atrial fibrillation (AF) with heart failure (HF) is prevalent, leading to severe complications. This review aimed to investigate the success rate and efficacy of cryoballoon ablation (CBA) by measuring the improvement in the New York Heart Association (NYHA) classification and the effect on the left ventricular systolic function in patients with AF accompanied by heart failure with reduced ejection fraction (HFrEF). Electronic databases search included PubMed, Web of Science, and Scopus in January 2023. Outcomes addressed the following: left ventricular ejection fraction (LVEF) improvement, AF recurrence, NYHA classification improvement, and mortality. STATA 17.0 software was used for data analysis. The effect size for the studies was a standard mean difference (SMD) with a 95% confidence interval (CI) for outcomes. Proportion analysis with 95% CI was used for freedom from early AF and AF after 2 years and all‐cause death. We included six studies, including 1699 HF patients with 365 HFrEF patients. The SMD of postoperative LVEF compared to preoperative LVEF in HFrEF was 0.99 ([95% CI: 0.60, 1.39], p = .00), and for NYHA was −1.12 ([95% CI: −1.36, −0.87], p = .00). The analysis results in HFrEF patients for freedom from AF after 1 year was 65% ([95% CI: 0.55, 0.75], and after 2 years was 39% ([95% CI: 0.10, 0.67]). Proportional analysis was conducted for all‐cause death, resulting in 3% mortality ([95% CI: −0.01, 0.07]). Cryoablation of AF accompanied by HFrEF appeared safe as it reduced AF recurrence and enhanced clinical outcomes.
Post‐Synthetic Modification of Triplex‐Forming Oligonucleotides Containing 2‐Aminoethyl‐2′‐Deoxynebularine Derivatives
Authors: Notomi Ryotaro , Sasaki Shigeki , Taniguchi Yosuke 
Published: October 2023

AbstractThis article describes the detailed synthetic protocol for the preparation of oligonucleotides containing 2‐guanidinoethyl‐2′‐deoxynebularine and 2‐ureidoethyl‐2′‐deoxynebularine nucleoside derivatives. These derivatives are obtained by a post‐synthetic modification of triplex‐forming oligonucleotides (TFOs) containing 2‐aminoethyl‐2′‐deoxynebularine, which is useful for forming stable triplex DNA with duplex DNA sequences containing 5mCG and CG interrupting sites. The hydroxyl groups of the sugar moiety of commercially available 2′‐deoxyguanosine are acetyl‐protected, the 6‐position is chlorinated and reduced to give a 2‐substituted nebularine derivative, and then the sugar moiety is deprotected. The hydroxyl groups of the sugar moiety are silyl‐protected and the amino group at the 2‐position is iodinated before being coupled with diethyl malonate. The ethyl ester is reduced and the resulting alcohol converted to an amino group for protection. The compound is then converted to a phosphoramidite unit and incorporated into a TFO. Subsequent modification of the aminoethyl group on the TFO completes the synthesis of the oligonucleotides containing 2‐guanidinoethyl‐2′‐deoxynebularine and 2‐ureidoethyl‐2′‐deoxynebularine. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Preparation of the phosphoramidite unit of the 2‐aminoethyl‐2′‐deoxynebularine derivative (14)Basic Protocol 2: Post‐synthetic modification of oligonucleotides containing 2‐aminoethyl‐2′‐deoxynebularine derivativesBasic Protocol 3: Determination of the triplex‐forming ability of oligonucleotides containing 2‐aminoethyl‐2′‐deoxynebularine derivatives
Correlations between different protein species of oral rinse MMP‐8 and patient‐related factors
Authors: Leppilahti Jussi M. , Tervahartiala Taina , Kautiainen Hannu , Räisänen Ismo , Ahonen Minna‐Maija , Uitto Veli‐Jukka , Sorsa Timo , Mäntylä Päivi 
Published: October 2023

AbstractObjectivesThe aim of this study is to examine correlations between different oral rinse matrix metalloproteinase (MMP)‐8 protein species in western blot (WB) analysis, quantitative MMP‐8 measurements, and patient‐related factors. Elevated activated MMP‐8 (aMMP‐8) associate with periodontitis and a diagnostic point‐of‐care technology has been developed based on aMMP‐8. In WB, different MMP‐8 protein species can be analyzed. Relative abundancy of fragmented 20–25 kDa forms in WB has been associated with and reflects MMP‐8 activation and related fragmentation and elevated quantitative aMMP‐8 measurements.Material and MethodsA random sample of 192 participants from a periodontal disease screening study was used for this study. Oral rinse samples for biomarker analyses were collected before clinical periodontal examinations. aMMP‐8 immunofluorometric (IFMA) and WB analysis (utilizing the same monoclonal antibody, 8708), polymorphonuclear leukocyte (PMN) elastase activity test and tissue inhibitor of metalloproteinases (TIMP)‐1 ELISA levels were performed from the oral rinse samples. Distinct MMP‐8 protein species were differentiated in the WB analysis. Principal component (PC) analysis was conducted to explore correlation patterns between the different species. Adjusted correlation analysis between the extracted PCs of WB and aMMP‐8 IFMA levels and multilevel regression analysis were conducted to explore if the other periodontal disease‐related biomarkers and clinical surrogate measures and patient‐related factors are co‐variating with the extracted components.ResultsDistinct correlation patterns between the MMP‐8 protein species were observed. The first four PCs explained 89% of the whole variance in PC analysis. Statistically significant correlation (p < 0.05) were observed as follows: PC1 positively with 21 kDa (r = .69) and 25 kDa fragments (r = .55) and negatively with 150 kDa complexes (r = −.46). PC2 correlated with 45 (r = .70) and 55 kDa (r = .65) activated forms, PC3 with 70–80 kDa latent proforms (r = .63) and 90–100 kDa complexes (r = .67), and PC4 with 35 kDa fragments (r = .81). There were significant correlations between quantitative (IFMA) aMMP‐8 measurements and PC1 (p < 0.001), PC2 (<0.05) and PC3 (<0.05) but not with PC4. In multilevel regression models age, PMN elastase activity, TIMP‐1 levels, and a number of 4–5 mm periodontal pockets were associated with PC1, nonsmoking with PC2, age and PMN elastase activity with PC3, and age and smoking with PC4.ConclusionsRelative abundancy of fragmented 21–25 kDa protein species was correlated with the quantitative aMMP‐8 (IFMA) measurements, which is in line with previous results. Different patient‐related factors (smoking, age, proteolytic activity) may modify the formation of different MMP‐8 protein species in oral rinse samples and may cause variability in quantitative aMMP‐8 measurement.
Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancer
Authors: Sun Xin , Ye Guiqin , Li Jiuzhou , Shou Huafeng , Bai Gongxun , Zhang Jianbin 
Published: October 2023

AbstractBackgroundInsulin‐like growth Factor 2 mRNA‐binding protein 3 (IGF2BP3) is a highly conserved RNA‐binding protein and plays a critical role in regulating posttranscriptional modifications.MethodsImmunoprecipitation was used to examine the interaction of Parkin and IGF2BP3. Mass spectrometry was performed to identify the ubiquitination sites of IGF2BP3. RNA‐immunoprecipitation was conducted to examine the target genes of IGF2BP3. Xenograft mouse model was constructed to determine the tumorigenesis of IGF2BP3.ResultsIGF2BP3 expression is negatively correlated with Parkin expression in human cervical cancer cells and tissues. Parkin directly interacts with IGF2BP3, and overexpression of Parkin causes the proteasomal degradation of IGF2BP3, while knockdown of PARK2 increases the protein levels of IGF2BP3. Mechanistically, in vivo and in vitro ubiquitination assays demonstrated that Parkin is able to ubiquitinate IGF2BP3. Moreover, the ubiquitination site of IGF2BP3 was identified at K213 in the first KH domain of IGF2BP3. IGF2BP3 mutation results in the loss of its oncogenic function as an m6A reader, resulting in the inactivation of the phosphoinositide 3‐kinase (PI3K) and mitogen‐activated protein kinase (MAPK) signalling pathways. In addition, IGF2BP3 mutation results in the attenuation of Parkin‐mediated mitophagy, indicating its inverse role in regulating Parkin. Consequently, the tumourigenesis of cervical cancer is also inhibited by IGF2BP3 mutation.ConclusionIGF2BP3 is ubiquitinated and regulated by the E3 ubiquitin ligase Parkin in human cervical cancer and ubiquitination modification plays an important role in modulating IGF2BP3 function. Thus, understanding the role of IGF2BP3 in tumourigenesis could provide new insights into cervical cancer therapy.
Neighborhood greenspace and neighborhood income associated with white matter grade worsening: Cardiovascular Health Study
Authors: Besser Lilah M. , Lovasi Gina S. , Zambrano Joyce Jimenez , Camacho Simone , Dhanekula Devi , Michael Yvonne L. , Garg Parveen , Hirsch Jana A. , Siscovick David , Hurvitz Philip M. , Biggs Mary L. , Galvin James E. , Bartz Traci M. , Longstreth W. T. 
Published: October 2023

AbstractINTRODUCTIONWe examined whether a combined measure of neighborhood greenspace and neighborhood median income was associated with white matter hyperintensity (WMH) and ventricle size changes.METHODSThe sample included 1260 cognitively normal ≥ 65‐year‐olds with two magnetic resonance images (MRI; ≈ 5 years apart). WMH and ventricular size were graded from 0 (least) to 9 (most) abnormal (worsening = increase of ≥1 grade from initial to follow‐up MRI scans). The four‐category neighborhood greenspace–income measure was based on median neighborhood greenspace and income values at initial MRI. Multivariable logistic regression tested associations between neighborhood greenspace–income and MRI measures (worsening vs. not).RESULTSWhite matter grade worsening was more likely for those in lower greenspace–lower income neighborhoods than higher greenspace–higher income neighborhoods (odds ratio = 1.73; 95% confidence interval = 1.19–2.51).DISCUSSIONThe combination of lower neighborhood income and lower greenspace may be a risk factor for worsening white matter grade on MRI. However, findings need to be replicated in more diverse cohorts.HIGHLIGHTS
Population‐based cohort of older adults (≥ 65 years) with greenspace and MRI data
Combined measure of neighborhood greenspace and neighborhood income at initial MRI
MRI outcomes included white matter hyperintensities (WMH) and ventricular size
Longitudinal change in MRI outcomes measured approximately 5 years apart
Worsening WMH over time more likely for lower greenspace‐lower income neighborhoods
Design, synthesis, and in‐silico study of novel triarylethylene analogs with dual anti‐estrogenic and serotonergic activity
Authors: Mostafa Tammy , Albeir Miriam , Wober Jannette , Abadi Ashraf , Salama Ismail , Ahmed Nermin S. 
Published: October 2023

AbstractEstrogen receptor is an important target in breast cancer. Serotonin receptors (5‐HT2A and 5‐HT2C, in particular) were investigated for a potential role in development and progression of breast cancer. Ligands that interact with estrogenic receptors influence the emotional state of females. Thus, designing selective estrogen receptor modulator (SERM) analogs with potential serotonergic activity is a plausible approach. The dual ligands can augment cytotoxic effect of SERMs, help in both physical and emotional menopausal symptom relief, enhance cognitive function and support bone health. Herein, we report triarylethylene analogs as potential candidates for treatment of breast cancer. Compound 2e showed (ERα relative β‐ galactosidase activity = 0.70), 5‐HT2A (Ki = 0.97 µM), and 5‐HT2C (Ki = 3.86 µM). It was more potent on both MCF‐7 (GI50 = 0.27 µM) and on MDA‐MB‐231 (GI50 = 1.86 µM) compared to tamoxifen (TAM). Compound 4e showed 40 times higher antiproliferative activity on MCF‐7 and 15 times on MDA‐MBA compared to TAM. Compound 4e had higher average potency than TAM on all nine tested cell line panels. Our in‐silico model revealed the binding interactions of compounds 2 and 2e in the three receptors; further structural modifications are suggested to optimize binding to the ERα, 5‐HT2A, and 5‐HT2C.
Plant provenance can influence the impacts of temperature and moisture on intraspecific competition in Pseudoroegneria spicata
Authors: Donnelly Sabina , Akin‐Fajiye Morodoluwa , Fraser Lauchlan H. 
Published: October 2023

AbstractWarming and changing precipitation can alter the performance of native grasses that are essential to grassland ecosystems. Native grasses may respond to changing climate by phenotypic plasticity or lose their current ranges. Establishing plant species from southern, warmer provenances may reduce the likelihood of biodiversity loss and improve restoration success in cool, northern locations that are undergoing warming. We conducted competition trials for Pseudoroegneria spicata (bluebunch wheatgrass), a native grass commonly found in western North American grasslands, to understand the impact of temperature and moisture on plant–plant interactions. We obtained seeds from three locations along a latitudinal gradient in North America, two in British Columbia (BC), Canada, and one in California, USA. We compared the effects of warming, changing water inputs, and competitor provenance on pairwise competitive interactions among Pseudoroegneria spicata plants grown from seeds obtained from the three locations. We quantified interactions using the relative interaction intensity, which has values from −1 (complete competition) to +1 (complete facilitation). Target plants from northern British Columbia, the location with the coldest summer temperature, were generally more competitively suppressed when competing with plants from California, which had the warmest summer temperature and lowest summer precipitation. Competitive suppression of target plants from northern British Columbia and southern British Columbia was more intense when competitor provenance was more geographically distant from target plant provenance. Finally, plants from northern British Columbia and southern British Columbia were more suppressed at higher temperatures, indicating some local adaptation, while plants from California were not affected by competitors, temperature, or water input. Plants grown from seeds obtained from warm and dry locations appear to be more tolerant to competition at higher temperatures, compared to plants from cooler regions. Native plant diversity and restoration success in grasslands subjected to climate change may be preserved or improved by assisted migration of seeds from warm to cooler but warming locations.