bluuebunny/crossref_metadata_2025_split huggingface dataset:
Possibility of evaluation of oxygen transport function in PAH patients on effective selexipag-based therapy
Authors: Allakhverdiev E. S. , Rezukhina E. A. , Slatinskaya O. V. , Rodnenkov O. V. , Maksimov G. V. , Martynyuk T. V. 
Published: October 2023

In the complex pathogenesis of idiopathic pulmonary arterial hypertension (IPAH), which includes changes in biochemical and biophysical processes in various cell types as the cause of structural and functional impairment, inflammation and thrombosis of the pulmonary vessels, hypoxia also plays an important role due to conformational changes in hemoglobin molecules with changes in their structure and oxygen transport function impairment.Aim: Using the method of Raman (Raman scattering) spectroscopy, to study changes in the conformation and ability of hemoglobin (Hb) to bind oxygen in blood erythrocytes from the cubital vein in IPAH patients and healthy people. Materials and methods: The study included 39 patients with newly diagnosed IPAH with functional class I – 2 patients, II – 13 patients, III – 22 patients, IV – 2 patients. 15 patients were treatment naive. In 24 patients a “washout” period of trial therapy was conducted for 5–7 days. The control group included 10 healthy volunteers.Results: It was established that the Raman spectra of Hb of whole blood erythrocytes and isolated red blood cells (RBC) of IPAH patients have significant differences from the control group. The IPAH group was characterized by an increase in the probability of finding heme in a «dome-shaped form», which is typical for the deoxygenated form of Hb, changes in the conformation of globin and the porphyrin macrocycle were revealed. A significant role in the change in the oxygen-transport function of Hb in IPAH was played by a change in the conformation of membrane-bound hemoglobin, which was characterized by an increase in the contribution of symmetrical vibrations of pyrrole rings in hemoporphyrin relative to the control, which required further study. Probably, the reason for the decrease in the ability of the oxygen transport function of membrane-bound hemoglobin was the decrease in the efficiency of electrostatic interaction with the protein of band 3. In clinical case positive changes in spectroscopy parameters were shown in IPAH patient with the effective use of selexipag.Conclusions: For the first time, the possibility of using a non-invasive method of Raman spectroscopy to study changes in the oxygen transport function of erythrocytes in IPAH patients has been proven, which can serve as an additional method in the diagnostic algorithm of this disease.
Case report: role of cardiac MRI in the diagnosis of myocarditis
Authors: Butorova E. A. , Stukalova O. V. 
Published: October 2023

Diagnosis of myocarditis remains one of the most difficult clinical problems in cardiology. In connection with the coronavirus infection COVID-19 caused by the acute respiratory virus SARS-CoV-2, non-invasive diagnosis of myocarditis is an urgent task. The most informative method for diagnosing myocarditis is magnetic resonance imaging [1,2]. The arsenal of MRI includes a number of pulse sequences that make it possible to identify and evaluate the process of inflammation in its various phases. Various pulse sequences (T2-weighted images or T2-mapping, delayed contrast, T1-mapping) allow not only to diagnose the disease, but also to determine the stages of damage. The ability of MRI to differentiate ischemic and non-ischemic lesions [3] is used in emergency cardiology for various myocardial injuries.The peculiarity of this clinical case is the use of MRI diagnostics in a patient with a typical clinical picture of acute coronary syndrome in the emergency department of cardiology. The data of the contrast MRI of the heart allowed the patient to make the correct diagnosis.
Host Genetic Variation Has a Profound Impact on Immune Responses Mediating Control of Viral Load in Chronic Gammaherpesvirus Infection
Authors: Holt Emily A , Waytashek Courtney M , Sessions Katherine J , Asarian Loredana , Lahue Karolyn G , Usherwood Edward J , Teuscher Cory , Krementsov Dimitry N 
Published: October 2023

Abstract
Chronic infection with the gammaherpesvirus EBV is a risk factor for several autoimmune diseases, and poor control of EBV viral load and enhanced anti-EBV responses elevate this risk further. However, the role of host genetic variation in the regulation of immune responses to chronic gammaherpesvirus infection and control of viral replication remains unclear. To address this question, we infected C57BL/6J (B6) and genetically divergent wild-derived inbred PWD/PhJ (PWD) mice with murine gammaherpesvirus-68 (MHV-68), a gammaherpesvirus similar to EBV, and determined the effect of latent gammaherpesvirus infection on the CD4 T cell transcriptome. Chronic MHV-68 infection of B6 mice resulted in a dramatic upregulation of genes characteristic of a cytotoxic Th cell phenotype, including Gzmb, Cx3cr1, Klrg1, and Nkg7, a response that was highly muted in PWD mice. Flow cytometric analyses revealed an expansion of CX3CR1+KLRG1+ cytotoxic Th cell–like cells in B6 but not PWD mice. Analysis of MHV-68 replication demonstrated that in spite of muted adaptive responses, PWD mice had superior control of viral load in lymphoid tissue, despite an absence of a defect in MHV-68 in vitro replication in PWD macrophages. Depletion of NK cells in PWD mice, but not B6 mice, resulted in elevated viral load, suggesting genotype-dependent NK cell involvement in MHV-68 control. Taken together, our findings demonstrate that host genetic variation can regulate control of gammaherpesvirus replication through disparate immunological mechanisms, resulting in divergent long-term immunological sequelae during chronic infection.
Netralitas Pegawai Negri Sipil (PNS) Dalam Pemilu Menurut Undang-Undang Nomor 10 Tahun 2008
Authors: Widhiana I Made Mandi 
Published: October 2023

In the legal and governmental aspects, civil servant bureaucracy reform is a very strong issue to be realized. This is because the Indonesian government bureaucracy is suspected to have contributed greatly to the occurrence of these various crises. By using the method of normative legal research a research method that discusses tie the norm angle in legal material. Furthermore, some of the problems that will be studied are 1) about the neutrality of civil servants in the General Election and 2) how to ensure the neutrality of civil servants themselves. By using a number of state legal theories, constitutional theories and legislative theories and theories of authority, and using the normative legal theory it will obtain discussion as follows:The bureaucracy built by the New Order government has fostered a bureaucratic culture that is thick with corruption, collusion and nepotism (KKN),Therefore bureaucratic reform is expected to be a corrective step towards the Government's political policy after the New Order.The conclusions obtained are: 1) the neutrality of civil servants in the reform of the bureaucracy, the Government has established new policies in coaching Civil Servants (PNS) as part of coaching 2) efforts to guarantee the neutrality of civil servants carried out with socialization and approach approach to the ceremony
PENGARUH JARAK TANAM DAN PENAMBAHAN BIOCHAR TERHADAP PERTUMBUHAN DAN JUMLAH ANAKAN PADI MSP 13 DI LAMPUNG TENGAH
Authors: Harini Nyang Vania Ayuningtyas , Lestari Dian , Dewantara Rio 
Published: October 2023

Salah satu upaya meningkatkan produksi padi di Indonesia dengan pemberian biochar dan penerapan jarak tanam pada padi MSP 13. Penelitian ini bertujuan untuk mengkaji pengaruh pengaturan jarak tanam dan aplikasi biochar terhadap tinggi tanaman padi MSP 13, dan jumlah anakan padi MSP 13. Rancangan yang digunakan yakni Rancangan Acak Kelompok (RAK) yang disusun secara faktorial (3x2) dengan 3 ulangan. Data dianalisis dengan sidik ragam dan dilanjutkan dengan Uji Beda NyataTerkecil (BNT) pada taraf 5%. Variabel yang diamati tinggi tanaman padi MSP 13 dan jumlah anakan padi MSP 13. Hasil penelitian menunjukkan bahwa perlakuan J1B1 pada saat padi berumur 20 HSS, 35 HSS, dan 65 HSS menghasilkan tanaman yang lebih tinggi dibandingan dengan perlakuan lain. Pada pengamatan jumlah anakan tanaman padi MSP 13 menunjukkan 20 HSS, 35 HSS dan 65 HSS berkisar antara 3,33 - 35 anakan per rumpun. Simpulan dari penelitian ini yakni perlakuan jarak tanam dan aplikasi biochar tidak berpengaruh terhadap tinggi tanaman padi MSP 13, perlakuan jarak tanam dan aplikasi biochar tidak berpengaruh terhadap jumlah anakan padi MSP 13, perlakuan B1 (pemberian biochar 5000 kg ha-1) mampu meningkatkan tinggi tanaman dan jumlah anakan padi MSP 13 dibandingkan dengan tanpa pemberian biochar, jarak tanam yang dianjurkan untuk budidaya tanaman MSP 13 yaitu 30x25 cm dan 25x25 cm.
The Identification of Common Druggable Targets For Acute Dysentery in Four Pathogenic Bacteria, Shigella, Salmonella, Campylobacter and E.Coli
Authors: Shafique Iqra , Shafique Hira , Asif Nimra , Liaquat Sadia , Shahbaz Husnain 
Published: October 2023

Purpose: Dysentery is a severe form of diarrhea and caused by four bacteria include Shigella, Campylobacter, E.coli and Salmonella. They are responsible for higher morbidity and mortality rates resulting from dysentery every year across the world. Antibiotic therapy of this disease plays a critical role in decreasing the prevalence as well as the fatality rate of this infection. However, the management of this disease remains challenging, owing to the overall increase in resistance against many antimicrobials. Hence, it has become important to identify as well as develop therapeutic methods presenting novel avenues for infections. In the current study, proteome based mapping was utilized to find the potential drug targets for dysentery. There is need to identify novel drug and vaccine targets to control this disease. This study is designed to identify new drug targets to develop drug and vaccines to battle bacillary dysentery. Subtractive genomics approach was used in this study to find novel drug targets.
Methodology: The proteomes of Shigella, E. coli, Campylobacter and Salmonella were retrieved from Uniprot. Paralogs in these proteomes were removed by CD-HIT. Gene essentiality was screened by Geptop server 2.0. Host-pathogen interaction was analyzed through HPIDB database. To identify non-homologous proteins, the essential proteins were analyzed in Blastp against Homo sapiens (H. sapiens). The unique metabolic pathways were recognized by comparison of metabolic pathways of selected strains of bacteria and H. sapiens using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Subcellular localization and functional classification of proteins involved in unique metabolic pathways were analyzed through PSORTb and SVMProt. Druggability potential of unique essential proteins was investigated using the DrugBank database.
Findings: Over all analysis showed 7 proteins that were common in all four bacteria. These proteins were essential to pathogens. Out of 7 proteins 6 proteins MURA, MURG, DAPA, MURB, DAPE, DnaA are reported as a drug target in different bacteria and one protein DAPD is novel. The study will enable the development of natural and cost-effective drugs against dysentery infections.
Recommendation: However, further validations are necessary to confirm their drug effectiveness and biocompatibility.
O PAPEL DA POLÍCIA MILITAR DO PARANÁ NA PROTEÇÃO DA MULHER VÍTIMA DE VIOLÊNCIA DOMÉSTICA: ESTUDO DE CASO A PARTIR DA FISCALIZAÇÃO DAS MEDIDAS PROTETIVAS NA QUARTA REGIÃO
Authors: Ferreira Clarindo Maximillian , Szczerepa Fabiano 
Published: October 2023

Em 2021, a 4ª Região de Polícia Militar do Paraná editou, com caráter inovador, o acompanhamento in loco de mulheres vítimas de violência doméstica e que possuem medida protetiva contra seus respectivos agressores. Trata-se de buscar a proteção deste grupo vulnerável com o intuito de evitar o cometimento de novas agressões e do feminicídio. Desta forma, passados dois anos desde a adoção desta postura, este artigo tem por objetivo central avaliar a eficácia da fiscalização das medidas protetivas na diminuição dos índices de descumprimento destas e na redução dos índices de violência doméstica na região. Para tanto, combina aspectos qualitativos e quantitativos, lançando mão do método dialético para interpretar os registros produzidos pelas equipes policiais neste período de acompanhamento. Também se buscou, na base de dados dos boletins de ocorrência, pelos índices destas violações, do ilícito da violência doméstica e dos feminicídios. Inobstante, o artigo faz um apanhado histórico da proteção à mulher no país, elencando os principais diplomas legais, avanços e possibilidades relacionadas com a proteção à mulher brasileira. A análise atesta para a complexidade do problema e para uma efervescência recente do Poder Público, que em diferentes segmentações tem se debruçado sobre o problema. Nesta direção, a fiscalização das medidas protetivas se mostra importante e eficiente na medida em que se observa tendência de redução dos índices criminais.
Tromboembolia pulmonar masiva en un paciente con enfermedad de Crohn y tuberculosis latente tratada con ustekinumab
Authors: Chávez-Sánchez Siomara Aransuzú , Cedrón-Cheng Hugo 
Published: October 2023

La enfermedad inflamatoria intestinal (EII) es un espectro de enfermedades crónicas inmunomediadas que afectan tanto el tracto gastrointestinal, como otros sistemas extraintestinales, comportándose como una enfermedad sistémica. Los fenómenos tromboembólicos son una complicación frecuente en la EII, como consecuencia de los estados de hipercoagulabilidad que se asocian con la actividad de la enfermedad, y su aparición tiene un impacto negativo tanto en el pronóstico como en la sobrevida de los pacientes. Debido a ello, el control de la actividad inflamatoria de la EII es uno de los pilares en el control de los eventos tromboembólicos. Los medicamentos biológicos se asocian al control rápido del cuadro inflamatorio, sin embargo, siempre se discute el tema de seguridad para la reactivación de infecciones latentes, en particular tuberculosis. Presentamos el caso de un paciente de 37 años que debutó con trombosis venosa profunda en el miembro inferior izquierdo y posteriormente con tromboembolismo pulmonar masivo. Luego de investigar la etiología y ampliar la historia clínica se le diagnosticó Enfermedad de Crohn (EC). Al realizar los estudios previos al uso de biológicos, las pruebas de PPD y quantiferon resultaron positivas, luego de la discusión del caso se decidió iniciar tratamiento con ustekinumab.
Radiofrequency Ablation for Chronic Knee, Hip, and Shoulder Pain
Authors: Shane Amanda , Bailey Sharon 
Published: October 2023

For patients with knee osteoarthritis (OA), radiofrequency ablation may reduce pain and improve function compared to other nonsurgical interventions without increasing adverse events.
There is insufficient evidence to suggest that radiofrequency ablation reduces pain or improves function among patients with chronic hip pain.
We did not find any studies or guidelines on the clinical effectiveness of radiofrequency ablation for treating chronic shoulder pain that met the inclusion criteria for this review.
Three guidelines conditionally recommend the use of radiofrequency ablation for patients with knee OA, and 1 guideline conditionally recommends the use of radiofrequency ablation for hip joint pain following diagnostic blocks.
ADOÇÃO INTERNACIONAL
Authors: Reis Márcia Maria Martins , Carvalho Filho Gilson Ribeiro 
Published: October 2023

O referente artigo ressaltará acerca da adoção internacional e os desafios encontrados nesta temática. Atualmente esta prática tem se tornado cada vez mais corriqueira, sendo um fator positivo para todos os infantes que necessitam de um lar adequado para viver. Posto isso, é necessário entender sobre o processo da adoção internacional, compreender os seus riscos e desafios, ressaltando também a ética que envolve este instituto, bem como a dificuldade para a adequação cultural no novo país. A metodologia a ser aplicada na referida pesquisa tem como base artigos científicos, livros e pesquisas específicas relacionadas ao tema em questão. Aspirando assim, para a compreensão acerca deste assunto e tornando embasada a adoção de crianças e adolescentes que necessitam de uma família com estrutura condizente e estável para o pleno desenvolvimento deste cidadão.
Total ozone variability and trends over the South Pole during the wintertime
Authors: Fioletov Vitali , Zhao Xiaoyi , Abboud Ihab , Brohart Michael , Ogyu Akira , Sit Reno , Lee Sum Chi , Petropavlovskikh Irina , Miyagawa Koji , Johnson Bryan J. , Cullis Patrick , Booth John , McConville Glen , McElroy C. Thomas 
Published: October 2023

Abstract. The Antarctic polar vortex creates unique chemical and dynamical conditions when the stratospheric air over Antarctica is isolated from the rest of the stratosphere. As a result, stratospheric ozone within the vortex remains largely unchanged for a 5-month period from April until late August when the sunrise and extremely cold temperatures create favorable conditions for rapid ozone loss. Such prolonged stable conditions within the vortex make it possible to estimate the total ozone levels there from sparse wintertime ozone observations at the South Pole. The available records of focused Moon (FM) observations by Dobson and Brewer spectrophotometers at the Amundsen–Scott South Pole Station (for the periods 1964–2022 and 2008–2022, respectively) as well as integrated ozonesonde profiles (1986–2022) and MERRA-2 reanalysis data (1980–2022) were used to estimate the total ozone variability and long-term changes over the South Pole. Comparisons with MERRA-2 reanalysis data for the period 1980–2022 demonstrated that the uncertainties of Dobson and Brewer daily mean FM
values are about 2.5 %–4 %. Wintertime (April–August) MERRA-2 data have a bias with Dobson data of −8.5 % in 1980–2004 and 1.5 % in 2005–2022. The mean difference between wintertime Dobson and Brewer data in 2008–2022 was about 1.6 %; however, this difference can be largely explained by various systematic errors in Brewer data. The wintertime ozone values over the South Pole during the last 20 years were about 12 % below the pre-1980s level; i.e., the decline there was nearly twice as large as that over southern midlatitudes. It is probably the largest long-term ozone
decline aside from the springtime Antarctic ozone depletion. While wintertime ozone decline over the pole has hardly any impact on the environment, it can be used as an indicator to diagnose the state of the
ozone layer, particularly because it requires data from only one station.
Dobson and ozonesonde data after 2001 show a small positive, but not statistically significant, trend in ozone values of about 1.5 % per decade that is in line with the trend expected from the concentration of the ozone-depleting substances in the stratosphere.
Characterization of refractory aerosol particles collected in the tropical UTLS within the Asian Tropopause Aerosol Layer (ATAL)
Authors: Ebert Martin , Weigel Ralf , Weinbruch Stephan , Schneider Lisa , Kandler Konrad , Lauterbach Stefan , Köllner Franziska , Plöger Felix , Günther Gebhard , Vogel Bärbel , Borrmann Stephan 
Published: October 2023

Abstract. Aerosol particles with diameters larger than 40 nm were collected during the flight campaign StratoClim2017 within the Asian Tropopause Aerosol Layer (ATAL) of the 2017 Monsoon Anticyclone above the Indian subcontinent. A multi-impactor system was installed on board of the aircraft M-55 Geophysica, which was operated from Kathmandu, Nepal. The size and chemical composition of more than 5000 refractory particles/inclusions of 17 selected particle samples from 7 different flights were analyzed by use of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) combined with energy dispersive X-ray microanalysis (EDX). Based on chemical composition and morphology, the refractory particles were assigned to the particle groups: extraterrestrial, silicates, Fe-rich, Al-rich, Hg-rich, other metals, C-rich, soot, Cl-rich, and Ca-rich. Most abundant particle groups within the refractory particles are silicates and C-rich (nonvolatile organics). In samples taken above the tropopause extraterrestrial particles are becoming increasingly important with rising altitude. The most frequent particle sources for the small (maximum in size distribution DP-max = 120 nm) refractory particles carried into the ATAL are combustion processes at ground (burning of fossil fuels / biomass burning) and the agitation of soil material. The refractory particles in the ATAL represent only a very small fraction (< 2 % by number for particles > 40 nm) of the total aerosol particles which are dominated by species like ammonium, sulfate, nitrate, and volatile organics. During one flight additionally a large number of very small (DP-max = 25 nm) cinnabar particles (HgS) were detected. These particles are most likely generated directly on ground by coal combustion in Northeastern India or Southern China. These findings show that coal burning is an important source for the entry of refractory particles and in particular mercury into the ATAL respectively in the upper troposphere/ lower stratosphere (UTLS) region.
Implementasi Kebijakan Pemungutan Pajak Reklame Di Kabupaten Garut
Authors: Hardianti Tantri , Alkautsar Muslim , Syakinah Fitri 
Published: October 2023

Tujuan penelitian ini bertujuan untuk mengetahui implementasi kebijakan pemungutan pajak reklame di Badan Pendapatan Daerah Kabupaten Garut. Teori yang digunakan dalam penelitian ini adalah Teori George Edward III. Pajak reklame adalah salah satu sumber pendapatan daerah yang sangat potensial untuk pembiayaan tinggi dari warga Negara Indonesia yang mempunyai kewajiban perpajakan dan juga sebagai salah satu upaya untuk mengoptimalkan pendapatan pajak daerah. Penelitian ini menggunakan jenis penelitian metodologi kualitatif pendekatan deskriptif. Hasilnya menunjukkan bahwa implementasi kebijakan pemungutan pajak reklame di Badan Pendapatan Daerah Kabupaten Garut sudah berjalan cukup baik diantaranya yaitu sumberdaya, disposisi dan struktur birokrasi. Sedangkan ditinjau dari komunikasi masih kurang optimal karena terdapat beberapa permasalahan yang ditemukan pada Badan Pendapatan Daerah. Selain itu terdapat kendala yang peneliti temukan dalam pemungutan pajak reklame ini seperti kurangnya sosialisasi tentang pemungutan pajak reklame dan kurangnya tingkat kesadaran wajib pajak untuk membayar pajak. Maka dari itu, upaya yang perlu dilakukan yaitu sosialisasi yang berkala oleh Badan Pendapatan Daerah Kabupaten Garut setidaknya 3 kali dalam setahun.
Reconstructing Society in the Light of Sūrat al-Ḥujurāt
Authors: Hossain ASM Delowar , Salama Muhammad 
Published: October 2023

Muslims are faced with many issues, including the malicious use of the tongue, that challenge their social cohesion. Considering the gravity of this matter, this work explores the path towards social reform by infusing the values of Sūrat al-Ḥujurāt into society leveraging the mechanisms of social reconstruction philosophy. In particular, it analyses verses 11 and 12 of Sūrat al-Ḥujurāt which primarily discuss tongue-related social ills such as backbiting, ridiculing, and name-calling; they also illustrate a process of self-development that can facilitate mutual respect and brotherhood thereby minimizing the likeliness of internal conflicts of the Muslims. This work utilizes a qualitative study, which demonstrates that Sūrat al-Ḥujurāt is instrumental in protecting society from the ills of the tongue.