bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tomato profilin Lyc e 1: IgE cross‐reactivity and allergenic potency
Authors: Westphal S. , Kempf W. , Foetisch K. , Retzek M. , Vieths S. , Scheurer S. 
Published: December 2004

Background:  To date, very little data are available about the nature of tomato allergens. Immunoglobulin E (IgE) cross‐reactive profilins have been suggested to account for allergic symptoms in patients suffering from tomato allergy.Methods:  The cDNA of tomato profilin was amplified by reversely transcribed polymerase chain reaction (RT‐PCR) from total RNA extracted from ripe tomato fruit. The gene was cloned into the pET101D expression plasmid and the protein was produced in Escherichia coli BL21. Purification was performed via poly‐l‐proline (PLP) affinity chromatography. IgE reactivity of recombinant tomato profilin was investigated by immunoblot and enzyme‐linked immunosorbent assay. IgE‐inhibition studies were performed to analyse cross‐reactivity with other profilins. To determine the allergenic activity of the recombinant protein, basophil histamine release assays using sera of patients with adverse reactions to tomato were performed.Results:  Profilin was identified as a new minor allergen in tomato fruits. The recombinant tomato profilin comprises 131 amino acids and high sequence identity to other allergenic food and pollen profilins. It was shown to be IgE‐reactive with a prevalence of 22% (11/50) in tomato‐allergic patients. In patients with tomato allergy and multiple sensitization to other foods and birch pollen, IgE directed against tomato profilin showed a strong cross‐reactivity with profilins from plant food sources and birch pollen. The tomato profilin was able to induce mediator release from human basophils.Conclusion:  The tomato profilin is a minor allergen in tomato fruit. Thus, it shows biological activity, as confirmed by in vitro histamine release assays with human basophils and thereby has the potential to account for clinical symptoms in tomato‐allergic patients.
Montelukast plus cetirizine in the prophylactic treatment of seasonal allergic rhinitis: influence on clinical symptoms and nasal allergic inflammation
Authors: Kurowski M. , Kuna P. , Górski P. 
Published: December 2004

Background:  The aim of our study was to investigate effects of 6‐week pretreatment of seasonal allergic rhinitis (AR) with cetirizine, and montelukast, alone and in combination. Antihistamine/antileukotriene treatment is effective in AR. Antihistamines may prevent AR symptoms while prophylactic activity of antileukotrienes remains unclear.Methods:  Sixty AR patients, aged 18–35 years, were randomized to receive placebo, montelukast only, cetirizine only, or montelukast plus cetirizine, 6 weeks prior and 6 weeks after the beginning of grass pollen season. Mean self‐recorded in‐season symptom scores and mean weekly all‐symptom scores were analyzed. In 31 patients, nasal lavages were performed before treatment, and at the end of the study, i.e. 12 weeks after the treatment initiation. Eosinophil and basophil counts, eosinophil cationic protein (ECP), and mast cell tryptase (MCT) levels were evaluated in lavage samples.Results:  Combined montelukast/cetirizine pretreatment significantly reduced in‐season symptom score for sneezing, eye itching, nasal itching, rhinorrhea, and congestion. Montelukast plus cetirizine were more effective than cetirizine alone in preventing eye itching, rhinorrhea, and nasal itching. Moreover, combined pretreatment with montelukast and cetirizine delayed appearance of AR symptoms. Eosinophil nasal lavage fluid counts were significantly increased during pollen season in placebo and montelukast‐only groups. No differences were observed in basophil counts. The in‐season ECP level was significantly increased in all groups except montelukast‐plus‐cetirizine group. In‐season MCT levels were not increased.Conclusion:  Combined antihistamine and antileukotriene treatment started 6 weeks before the pollen season is effective in preventing AR symptoms and reduces allergic inflammation in nasal mucosa during natural allergen exposure.
Cross‐reactivity between obeche wood (Triplochiton scleroxylon) and natural rubber latex
Authors: Venturini M. , Gastaminza G. , Kespohl S. , Bernedo N. , Garmendia M. , Raulf‐Heimsoth M. , Muñoz D. , Ansotegui I. J. 
Published: December 2004

Background:  Several studies have shown that allergy to natural rubber latex is associated with cross reactivity to other plants. We have investigated a possible cross reactivity with a tree belonging to the Sterculiaceae family, Tryplochiton scleroxylon, in a patient allergic to obeche wood, that began to suffer symptoms of allergy in contact with latex.Methods:  Determinations of specific IgE by CAP to obeche, natural rubber latex (NRL) and recombinant allergens of latex were done. Immunoblotting was performed to study allergens detected by serum of our patient. CAP‐inhibition methods were used to study cross‐reactive between NRL and obeche wood.Results:  In obeche extract, 4 bands were found of an apparent molecular weight of 18, 38, 75 and 199 kDa. In NRL extract the allergens have a molecular weight around 18, 21, 32, 38, 60, 70 and 199 kDa. IgE to latex was almost completely inhibited (96%) by the obeche extract in CAP‐inhibition studies. Using obeche extract as inhibitor, IgE binding to native hevein (nHevb 6.02) was reduced by 25%.Conclusions:  The results obtained in CAP‐inhibition demonstrate the existence of cross‐reactivity between proteins in NRL and obeche wood. In our patient, a band of apparent molecular weight of 38 kDa could be the most important allergen.
Magnetic resonance imaging analysis of amygdala and other subcortical brain regions in adolescents with bipolar disorder
Authors: DelBello Melissa P , Zimmerman Molly E , Mills Neil P , Getz Glen E , Strakowski Stephen M 
Published: December 2004

Objectives:  Few studies have examined the abnormalities that underlie the neuroanatomy of bipolar disorder in youth. The aim of this study was to evaluate brain regions that are thought to modulate mood utilizing quantitative analyses of thin‐slice magnetic resonance imaging (MRI) scans of adolescents with bipolar disorder. We hypothesized that adolescents with bipolar disorder would exhibit abnormalities in brain regions that are involved in the regulation of mood including the amygdala, globus pallidus, caudate, putamen, and thalamus.Methods:  Bipolar adolescents (n = 23) and healthy subjects (n = 20) matched for age, race, sex, socioeconomic status, IQ, education and Tanner stage, were evaluated using the Washington University at St Louis Kiddie‐Schedule for Affective Disorders and Schizophrenia (WASH‐U K‐SADS). Contiguous 1 mm axial T1‐weighted MRI slices were obtained using a GE 1.5 T MR scanner. Regions of interest (ROI) included total cerebral volume, amygdala, globus pallidus, caudate, putamen, and thalamus.Results:  Total cerebral volume was smaller in bipolar adolescents than in healthy adolescents. A MANCOVA revealed a significant group difference in overall ROI volumes after adjusting for total cerebral volume. Specifically, adolescents with bipolar disorder exhibited smaller amygdala and enlarged putamen compared with healthy subjects.Conclusions:  Our findings indicate that adolescents with bipolar disorder exhibit abnormalities in some of the brain regions that are thought to be involved in the regulation of mood. Additional structural and functional neuroimaging investigations of children, adolescents, and adults with bipolar disorder are necessary to clarify the role of these brain regions in the neurophysiology of adolescent bipolar disorder.
Stratigraphy, sedimentary development and palaeoenvironmental context of a naturally accumulated pitfall cave deposit from southeastern Australia
Authors: Kos A. M. 
Published: December 2004

McEachern’s Deathtrap Cave (G‐49/50) is located in the Lower Glenelg region of southeastern Australia and records a Late Pleistocene to Holocene sedimentary record that has been directly influenced by surface processes during its formation. The sedimentary sequence contained within the cave is divided into lower, middle and upper sequences consisting of eight facies. The lower sequence represents the earliest phase of sedimentation, and groundwater fluctuations during the Last Interglacial period resulted in its erosion and redistribution deeper into the cave system. A decrease in the magnitude and frequency of flood events in the cave during the formation of the middle sequence indicates increasingly drier surface conditions prior to the Last Glacial Maximum. The middle sequence has a minimum age of 9840 ± 290 a BP. Moving sand sheets during the Last Glacial blocked the entrance to the cave allowing flowstones to develop on the cave floor. The surface environment surrounding the cave was probably not as dry as contemporaneous inland sites because sedimentation continued to be dominated by flowing water during this period. Holocene sedimentation is represented by the upper sequence and reflects wetter cave conditions between 7680 ± 160 a BP and 5700 ± 110 a BP. A major phase of sediment accretion occurs after 5700 a BP and correlates to a phase of dune instability in the Lower Glenelg region. Flowing water remodelled the sediment cone sometime after 2240 ± 100 a BP, which represents a period of increased surface runoff, although it is not clear whether this is due to climatic or anthropogenic influences.
Basin setting and age of the Late Palaeoproterozoic Capricorn Formation, Western Australia
Authors: Hall C. E. , Powell C. McA. , Bryant J. 
Published: December 2004

The Palaeoproterozoic Capricorn Formation near Ashburton Downs in northwestern Australia formed during the latter stages of the convergence of the Pilbara and Yilgarn Cratons. Palaeocurrent and facies analyses show that the southwesterly derived sediments were deposited in terrestrial environments and in a lake or shallow sea with a shoreline trending southeast. Intraformational debris flows suggest instability during sedimentation. Zircon grains from an accretionary lapilli tuff, dated at 1804 ± 7 Ma by the SHRIMP U–Pb method, show that the Capricorn Formation was deposited at the same time as granitic plutons were intruded in the Gascoyne Complex to the south and west. Although the Capricorn Formation was deposited with marked angular unconformity over the turbiditic Ashburton Formation, both formations could have been deposited in a foreland basin on the northeast flank of the growing Ashburton Fold Belt.
Two age populations of zircons from the Timber Creek kimberlites, Northern Territory, as determined by laser‐ablation ICP‐MS analysis
Authors: Belousova E. A. , Griffin W. L. , Shee S. R. , Jackson S. E. , O'Reilly S. Y. 
Published: December 2004

Two populations of kimberlitic zircon are present in the Timber Creek kimberlites, Northern Territory. Laser‐ablation ICP‐MS U–Pb dating yields an age of 1483 ± 15 (2σ) Ma for the main group and an age of 179 ± 2 Ma for the other group. This distinction of two age groups is strongly supported by Hf isotope data on the same zircons. Although the trace‐element patterns of both populations are typical of mantle‐derived zircons, the ‘young’ population has slightly higher concentrations of most trace elements, but has lower Hf, Nb, Ta and Pb contents. The distinct differences in trace‐element contents and Hf isotopic composition of the two zircon populations indicate that they were derived from different magma sources. The dating results indicate that the emplacement age of the Timber Creek kimberlites cannot be older than the age of the ‘young’ zircon population (i.e. 179 ± 2 Ma). This clarifies the inconsistency between the previously reported SHRIMP age of the Timber Creek zircons (1462 ± 53 Ma) and the much younger age (1200 Ma) of the sediments of the Victoria River Basin into which these kimberlites have intruded. The Timber Creek kimberlites are a newly recognised extension of the widespread Jurassic kimberlite activity known in Western Australia and South Australia (Wandagee, Orroroo, Cleve and Eurelia kimberlites).
1.2 Ga Mafic dyke near York, southwestern Yilgarn Craton, Western Australia
Authors: Pidgeon R. T. , Nemchin A. A. 
Published: December 2004

A SHRIMP 207Pb/206Pb zircon age of 1204 ± 10 Ma is reported for an east–west‐trending dolerite dyke from near York in the southwestern Yilgarn Craton. This age is identical within analytical uncertainty to previously reported ages of ca 1210 Ma for dykes from the central Wheatbelt and the Western and Eastern Goldfields. The consistency of the dyke ages and the wide areal extent of the dykes suggests that emplacement occurred as a single magmatic pulse at ca 1210 Ma throughout the southwestern Yilgarn Craton. The similarities between the age of the dykes and the ages of late events in the Albany–Fraser Orogeny, and the approximate parallelism of the east–west‐trending dykes to the margin of the orogen, raises the possibility that these events are related.
Actinic prurigo: A retrospective analysis of 21 cases referred to an Australian photobiology clinic
Authors: Crouch Rohan , Foley Peter , Baker Christopher 
Published: December 2004

SUMMARYActinic prurigo (AP) is a rare acquired idiopathic photodermatosis, reported most often in American Indians, but also in Caucasian and Asian populations. The skin lesions in AP predominantly affect exposed sites but may involve covered areas, and often result in postinflammatory scarring. The diagnosis of AP can be difficult and relies on a combination of history, clinical experience and investigations including phototesting and human leucocyte antigen typing. Twenty‐one patients (17 women, four men) diagnosed with AP at the photobiology clinic at St Vincent’s Hospital Melbourne were reviewed in this retrospective study. The mean age of patients at presentation to the clinic was 25 years, with the mean age of onset being 14 years. Phototesting was undertaken in 20 patients, with 12 (60%) having reduced and eight (40%) normal minimal erythema doses. Human leucocyte antigen typing indicated 18 patients (85.7%) were DR4 positive, with further subtyping of the DR4 allele establishing that 15 patients (71.4%) were DRB1*0407 positive and that two (9.5%) were DRB1*0401 positive. This condition is often recalcitrant, with treatment options including photoprotection, topical and oral corticosteroids, antimalarials, phototherapy and thalidomide.
Ticks in Australia
Authors: Storer Emma , Sheridan Adam T , Warren Lachlan , Wayte Jeffrey 
Published: December 2004

SummaryTicks are blood‐sucking parasites of vertebrates that may embed in human skin and are therefore of clinical relevance to dermatologists and their medical colleagues. Depending on the species involved, consequences of tick attachment vary from minor local reactions to significant systemic sequelae. It is possible to minimize morbidity by removing the tick in its entirety as soon as it is detected. Some techniques to achieve this are described. This review will aid clinicians in the recognition and practical management of tick bites in Australia.
Diagnosis and treatment of the common idiopathic photodermatoses
Authors: Ferguson James 
Published: December 2004

SummaryThe idiopathic photodermatoses are the most common cause of photosensitivity and the commonest of these are polymorphic light eruption, actinic prurigo, chronic actinic dermatitis and solar urticaria. The clinical presentation, investigation and treatment of these four disorders are presented. Spontaneous improvement does occur.
Treatment of acne with isotretinoin: Recommendations based on Australian experience
Authors: Cooper Alan J ,   Australian Roaccutane Advisory Board
Published: December 2004

SummaryOral isotretinoin revolutionized acne treatment when it was introduced in 1982 in the USA. However, its use was restricted to patients with severe nodulocystic acne. Today its use worldwide has expanded to treat also patients with less severe but scarring acne who are responding unsatisfactorily to conventional therapies. These recommendations assess the potential for use of oral isotretinoin as a safe and effective treatment for severe nodulocystic acne unresponsive to conventional therapy, and acne of any severity that causes scarring or is associated with psychological distress.
PUVA treatment of alopecia areata totalis and universalis: A retrospective study
Authors: Whitmont Kaley J , Cooper Alan J 
Published: December 2004

SummaryThe results of PUVA treatment of alopecia areata (AA) totalis and universalis were reviewed in 26 adult patients. Eight of 15 patients with AA totalis and six of 11 patients with AA universalis achieved a complete response (>90% hair regrowth). Patients with AA totalis had a greater incidence of treatment failure (<25% hair regrowth) than those with AA universalis. Patients with a family history of AA were significantly less likely to have a positive response to PUVA than those with no family history. Sex, age at diagnosis and treatment, interval between diagnosis and treatment, and background of atopy were not significant determinants of outcome. Although unable to show significance for clinical response to treatment, this study demonstrates complete hair regrowth in patients with both AA totalis (53%) and universalis (55%) while reporting a low relapse rate among these patients (21%) within a long period of follow up (mean 5.2 years).
Clinical study of primary cutaneous B‐cell lymphoma using both the European Organization for Research and Treatment of Cancer and World Health Organization classifications
Authors: Yap Lee Mei , Blum Robert , Foley Peter , McCormack Christopher , Turner Hugh , Seymour John F , Prince H Miles 
Published: December 2004

SummaryPrimary cutaneous B‐cell lymphoma (PCBCL) is rare, with few series reported in the literature. Its classification and treatment remain controversial. Biopsy specimens of 13 patients with PCBCL were classified according to both the European Organization for Research and Treatment of Cancer (EORTC) and the new World Health Organization (WHO) classifications. Treatment and clinical outcomes were documented. Using the EORTC classification there were seven men and six women aged 32–85 years (mean = 51 years) with follicle centre cell (FCC) lymphoma (nine), immunocytoma (two) and primary cutaneous large B‐cell lymphoma of the leg (PCLBCL‐leg) (two). When the WHO classification was used, the nine patients with FCC were reclassified as follicle centre (five) and diffuse large B‐cell lymphoma (four). Most patients had localized disease (12). Initial treatment consisted of radiotherapy alone (seven), combination chemotherapy alone (one), combined chemoradiotherapy (three) and surgery (two). Twelve patients achieved complete remission (median follow up 28 months, range 10–167 months). One patient with PCLBCL‐leg died from progressive cutaneous disease. Most localized PCBCL lesions (except PCLBCL‐leg) have a favourable prognosis. We recommend that clinicians be familiar with the important differences in the EORTC and WHO classifications. Further large prospective studies comparing the WHO and EORTC classifications are required to more clearly delineate the outcomes of the increasing number of patients who are classified as DLBCL by the WHO system.
Treatment of severe recalcitrant plaque psoriasis with single‐dose intravenous tumour necrosis factor‐alpha antibody (infliximab)
Authors: Chan Jonathan J , Gebauer Kurt 
Published: December 2004

SummaryInfliximab is a chimeric anti‐tumour necrosis factor‐α antibody that has been demonstrated to have marked efficacy in the treatment of psoriasis. Seven patients with chronic plaque psoriasis were treated with single‐dose intravenous infliximab (5 mg/kg), and the Psoriasis Area and Severity Index (PASI) and Dermatology Life Questionnaire Index (DLQI) were used as a measure of treatment efficacy. There was an average improvement in PASI scores of 69% at 2 weeks post infusion. There was an improvement in DLQI of 61%. Four of the seven patients were also seen at 10 weeks post infusion and the improvement in PASI and DLQI was sustained. All patients tolerated the initial infusion well without adverse events. The results indicate that single‐dose infliximab is an effective and efficacious therapy for recalcitrant psoriasis and has a prolonged therapeutic effect.
Genetic testing of the family with a Carney‐complex member leads to successful early removal of an asymptomatic atrial myxoma in the mother of the patient
Authors: Aspres Nicholas , Bleasel Narelle R , Stapleton Karen M 
Published: December 2004

SummaryCarney complex is a rare cardiocutaneous syndrome with an autosomal‐dominant inheritance pattern. Apart from its cutaneous manifestations of multiple blue naevi and lentigines, it can involve multiple other organ systems, particularly the heart, where myxoma tumours commonly develop and can potentially lead to serious complications such as cerebrovascular accidents and myocardial infarction. Recently, a specific mutation in the gene encoding the R1‐α regulatory subunit of cyclic adenosine monophosphate‐dependent protein kinase A (PRKAR1α) has been discovered and found to be associated with a high risk of developing cardiac myxomas. We report the case of a Carney‐complex family member who displayed no observable clinical or cardiac features of the disease but who was found to be positive for the PRKAR1α gene mutation on genetic testing. Further evaluation of this patient subsequently led to the discovery of a 3‐cm atrial myxoma that had previously been undetected on cardiac assessment. This case highlights the potential benefits of using genetic screening for this disease.
Treatment of Bowen's disease and basal cell carcinoma of the nose with imiquimod 5% cream
Authors: Wu Jason K , Siller Greg , Whitehead Kevin 
Published: December 2004

SummaryWe report the successful use of topical imiquimod 5% cream for extensive multifocal, recurrent (post cryotherapy), biopsy‐proven Bowen's disease of the nose. Treatment was applied on a once‐a‐day regimen, and a total of 32 applications over 9 weeks were used. A florid local skin reaction occurred early in the treatment, necessitating a rest period and decreasing the frequency of application. The Bowen's disease was coexistent with a multifocal superficial basal cell carcinoma (BCC) that had a partial response. Persistent BCC at 4 weeks post treatment was surgically excised. This tumour showed an unusual histological picture, with normal epidermis overlying residual BCC in the papillary dermis. The Bowen's disease remains clinically clear at 12‐months follow up.
Recurrent morphoeic basal cell carcinoma at the lateral canthus with orbitocranial invasion
Authors: Selva Dinesh , Hale Lloyd , Bouskill Keith , Huilgol Shyamala C 
Published: December 2004

SummaryWe present a case of recurrent, nodulocystic and morphoeic basal cell carcinoma (BCC) at the lateral canthus, with clinically silent orbital and intracranial invasion which was treated with radiotherapy. Orbital invasion is a rare complication of periocular BCC and occurs primarily in high‐risk canthal lesions. Preoperative ophthalmological review and imaging should be considered in these cases.