bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphological characterization and screening of Impatiens wild species for heat tolerance
Authors: Kartikaningrum S , Mawaddah  , Pramanik D , Dewanti M , Badriah DS , Shintiavira H , Diningsih E , Hanudin  , Nuryani W , Sihombing D , Handayati W , Nurdina D , Ariantika S , Soehendi R , Yufdy MP , Utami N 
Published: November 2023

Abstract

Impatiens sp. is an annual plant that flowers all year round. High temperature stress is a major challenge limiting crop productivity. The research was conducted at experiment garden in Palasari Cipanas, BRIN, Indonesia, at an altitude of 1000 ASL, from January - April 2023. The aim of the research was to explore the morphological variation of Impatiens wild species and to obtain some heat tolerance accession for next breeding program. Twenty nine accession of Impatiens were observed for the qualitative and quantitative data including the evaluation of heat tolerance. Twelve qualitative characters were recorded. The qualitative characters were analyzed as degree of similarity. DICE Similarity coefficient of qualitative data was 0.06. Broad variability in the genotypic, phenotypic variances and genetic advance was found for leaf length, leaf width and heat tolerance score. Of the electrolyte leakage analyses, it was found that flower length, ventral petal length width, lateral petal length and spur length positively support the high tolerance of Impatiens accession to heat stress. The darker color of the stem, anther, and bract is followed by a reduction in the dimension of flower width, lateral petal width, dorsal petal width and spur length but the increased leaf thicknesses. There was no direct correlation between the heat tolerance score and the leaf thickness. The largest data diversity explained by 3 factors with a total variance of 68.88 %.
Total phenolic and flavonoid contents of Aphanamixis polystachya (Wall.) R.Parker leaf extract and its potential as antioxidant and inhibitor of α-glucosidase
Authors: Wibawa I P A H , Hanafi M , Li’aini A S , Rahayu A , Lugrayasa I N , Butardo V M , Mahon P J 
Published: November 2023

Abstract
Indonesia is one of the countries with the highest plant diversity in the world, including the Aphanamixis genus, and they use the plants in this genus as traditional medicine. The genus Aphanamixis has a wide variety of compounds with different activities, such as insecticidal, leishmanicidal, antimicrobial, anticancer, cytotoxic against some cancer cells, and antioxidant The focus of this research is to find out about the anti-oxidant, anti-diabetic, and anti-bacterial activities of Aphanamixis polystachya leaf extract from the Bali Botanical Garden plant collection. The antioxidant activity assay was carried out by scavenging the free radical 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenyl ethylamino) propane hydrochloride (DPPH). The antidiabetic assay was carried out by inhibiting the activity of the α-glucosidas eenzyme, while the antibacterial test was carried out by the agar diffusion method. Based on the data generated in this study, the active ingredients of A. polystachya leaf extracts have high potency as antioxidants and α-glucosidase enzyme inhibitors, but the extract was not effective in inhibiting bacterial growth.
Isolation of potential Zn solubilizing bacteria from corn rhizosphere
Authors: Irmadamayanti A , Jayadi M , Mantja K 
Published: November 2023

Abstract
Deficiency of micronutrients is a limiting factor in crop productivity. In terms of micronutrient deficiencies, zinc (Zn) deficiency is considered the most common. Obstacles to the availability of Zn in plants are not caused by the low total concentration of Zn, but by the low solubility of Zn in the soil. Therefore, a feasible approach is to exploit the capacity of soil microorganisms, especially bacteria, to convert the insoluble form of Zn to the soluble form of Zn, thereby increasing its availability and ability of plants to absorb it. This study aims to isolate various bacteria with the potential to dissolve Zn. Bacterial isolates obtained from the rhizosphere of corn plants were tested on insoluble zinc compounds, such as ZnCO3, ZnO, and Zn3(PO4)2 separately. Dissolving potential was assessed qualitatively by observing the formation of transparent zones. Of the 56 types of bacteria tested, only 6 isolates formed transparent zones. The results showed variations in the efficiency of dissolving insoluble Zn compounds between bacteria and the Zn sources used. Isolate Btg.2.3 showed the widest transparent zone in Zn media (ZnO, ZnCO3, and Zn3(PO4)2) with sizes of 20.0 mm, 24.0 mm and 15.7 mm, while isolate Bn.1.7 showed the smallest transparent zone with sizes 14.0 mm, 14.3 mm and 8.7 mm. The area of the transparent zone indicates the level of solubility and can be related to the dissolution of soluble Zn compounds produced by bacterial isolates. Of the Zn sources used, ZnCO3 showed the highest formation of transparent zones, followed by ZnO and Zn3(PO4)2.
Tissue Culture of Four Varieties of Date Palm Grown in Indonesia
Authors: Sinta M M , Saptari R T , Riyadi I , Sumaryono  
Published: November 2023

Abstract
In vitro propagation of date palms adaptive to Indonesian agroclimate is one of the solutions for providing superior date palm planting material. This study aimed to induce embryogenic calli and regenerate the calli into plantlets of four varieties of date palm grown in Indonesia: three female varieties (Barhee, Ajwa, and Khalas) and one male variety (Jarvis). The materials used were offshoots of the date palm trees that had been flowered at the Jonggol Farm, Bogor, Indonesia. Four layers (layer 1 is the innermost layer and 4 is the outermost layer) of the offshoot were cultured on MMS media added with 100 mg/L 2,4-D combined with 1 and 3 mg/L 2-iP. The results showed that primary callus for var Bahee and Ajwa emerged at 12 weeks after culture. Barhee and Ajwa, and at 18 weeks for Jarvis and Khalas. In var. Jarvis and Khalas, calli were induced only in layer 1 cultured on the media with 1 and 3 mg/L 2-iP. In comparison, callus induction of var. Barhee date palm was successfully performed in layer 1 and 2 on the media with 1 mg/L 2-iP. On the contrary, callus induction in var. Ajwa was successful in layer 1 and layer 2, on the media supplemented both with 1 or 3 mg/L 2-iP. Germination of the date palm somatic embryos into shoots, using non-hormone media occurred successfully in var. Ajwa, Jarvis, and Khalas, but not in Barhee. Regeneration of the shoots into plantlets was carried out successfully only in var. Ajwa and Jarvis. In conclusion, in vitro propagation of date palm was successfully conducted in Jarvis and Ajwa varieties, while var Barhee was the most difficult among the four varieties.
Recollection and conservation of Vatica javanica subsp. scaphifolia, a critically endangered endemic tree species in Southeastern Borneo
Authors: Primananda E , Pratama B A , Susanti R , Wibisono Y , Hamidi A , Rinandio D S , Robiansyah I 
Published: November 2023

AbstractVatica javanicasubsp.scaphifolia(Kosterm.) P.S.Ashton (Dipterocarpaceae) is an endemic tree confined to Southeastern Borneo. The International Union for Conservation of Nature (IUCN) Red List classifies the species as Critically Endangered (CR) based on the assessment conducted 25 years ago. The species is not collected yet in any ex-situ conservation areas in the world. After more than 3 decades since the last herbarium specimen was collected in 1991, here this study reported the recollection and new locations ofV. javanicasubsp.scaphifoliain Bukit Bangkirai Forest, Kutai Kartanegara Regency, East Kalimantan Province. The confirmation of the species involved a comparison between the newest collected specimens and living photos and the specimen type and key to the species in the protologue. The present research provides new information on the morphology of the tree species through photos of the wild living plants. Using an exploration survey method, this research recorded the population in two locations with elevation ranges of 70-95 m. The field survey located a total of 13 immature individuals with height and diameter ranges of 2.1-14.7 m and 1-9.7 cm, respectively. The study observed that forest conversion into rubber plantations(Hevea brasiliensis)and open coal mining are the main threats to the species. Conservation of the species has been done by collecting plant materials to serve as the first ex-situ living collection at the Bogor Botanic Gardens BRIN.
Dendrobium sp. subcultures with instant Vacin Went media against many concentrations of fruit waste liquid organic fertilizer plus
Authors: Juwaningsih E H A , Hasan A , Pandjaitan C 
Published: November 2023

Abstract
Orchids are ornamental plants in great demand by collectors and the public due to the varieties colors of Orchid flowers and the price. This study aims to determine the effect and obtain the concentration of the application of fruit waste liquid organic fertilizer plus on the growth and development of a Dendrobium sp. sub-culture on VW media in vitro. The research had been carried out from May to August 2022 at the Biotechnology Laboratory of Kupang State Agricultural Polytechnic. The study design used a single-factor complete randomized design consisting of six treatments and four replications. The tested factors were six level of concentrations of fruit waste liquid organic fertilizer plus instant VW media in vitro, which are: instant VW media; instant VW media + 2.5 ml/L; instant VW media + 5 ml/L; instant VW media + 7.5 ml/L; instant VW media + 10 ml/L; and instant VW media + 12.5 ml/L. The results showed that the best concentration of Fruit Waste Plus LOF for the growth of Dendrobium orchid explants is the treatment instant VW media + 7.5 ml/L LOF, which is shown by the time of bud emergence (10.75 days after planting), number of shoots (2.38), shoot height (3.5 mm), number of roots (1.38), and root length (2.5 mm).
Effect of various LED light on the growth and reproductive of in vitro-derived Indonesian chili pepper (Capsicum annum) Kopay and Laris varieties
Authors: Sulistyowati Y , Wahyuni  , Hartati NS , Kim J , Cho E , Seskar M , Raskin I , Sudarmonowati E 
Published: November 2023

Abstract
Red chili (Capsicum annuum), is one of the horticultural commodities that has a high demand. The production, however, is limited because of unsuitable climate and pest attack. Artificial light using LED light in indoor farming is a potential approach to solve the problem and for the production of metabolites for human health. The aim of this research was to evaluate the effect of various LED lights (white, red, blue, UV and combination red+blue) on two Indonesian chili pepper (Capsicum annuum) varieties Kopay and Laris derived from in vitro cultures and grown in the growth chamber and the greenhouse. Parameters observed were leaves number, plant height, number and weight of axillary buds, number of flower and number of young fruits. The results showed that Laris and Kopay under red and blue light significantly different on height character at 66 DAT. In Laris variety, the highest leaves number observed in plants under red light, while the highest young fruit number observed under blue light. Both red and blue lights grew taller than white light. In Kopay variety, combination red+blue light resulted in higher value in plant height, number of flowers has bloomed, number of axillary buds and weight of axillary buds at 37 DAT. The highest young fruit number observed in plants under white light. These results demonstrated that light wavelength through LED light affected plant growth and development. The findings are crucial for determining the best treatment for producing fruits containing health related-metabolites accumulated in chili peppers of each variety.
Autoinduction expression of Bst DNA polymerase using lac operon-controlled expression systems in Escherichia coli
Authors: Laksmi F A , Nuryana I , Vivid C , Hadi M I 
Published: November 2023

Abstract
Autoinduction simplified the steps for protein production by omitting the need to monitor the correct time for addition of inducer. This method is preferable for industrially production of protein. By using lac operon-controlled expression system, it is possible to produce the protein without the addition of IPTG as inducer. In a conventional way, IPTG was added at the exponential growth phase. By using autoinduction, IPTG could be replaced by lactose in the lac operon-controlled expression systems. Lactose was already supplemented in the medium and it was more cost efficient than IPTG. Therefore, this study aims to employ autoinduction method for expression of our protein target as it is a more convenient way and it is suitable for industrial application. In this study, DNA polymerase from Geobacillus stearothermophilus (Bst DNA polymerase), an enzyme which generally used in loop-mediated isothermal amplification (LAMP) assay, was expressed using lac operon-controlled expression systems in Escherichia coli. Bst DNA polymerase was successfully expressed using autoinduction expression method in LB medium containing 0.2% Lactose.
Embryogenic callus induction of Indonesian Cassava (Menti and Adira 4) on different picloram concentrations
Authors: Azizi A A A , Rahman N , Hartati N S , Koerniati S , Hastilestari B R , Sukmadjaja D , Witjaksono  
Published: November 2023

Abstract
Cassava (Manihot esculenta Crantz) is an important staple crop in tropic countries. A reproducible somatic embryogenic callus production will be useful in the biotechnology breeding program for plants including cassava. Menti and Adira-4 are two types of high yield cassava genetic resources in Indonesia. Immature leaf lobes from cassava in vitro were used as explants on MS basal medium with five level concentrations of Picloram: 0 mg L−1, 3 mg L−1, 5 mg L−1, 10 mg L−1, and 12 mg L−1. Initial embryogenic callus were seen on the explants in 2 weeks after treatment. Explants were then subcultured to the same medium every three weeks until three cycles of subculture to proliferate pro-embryos. Result showed that MS basal medium containing picloram 3 – 12 mg L−1 could induce embryogenic callus formation in Menti and Adira 4 immature leaf lobe explants. The highest percentage of explants forming embryogenic callus was on D medium (MS + 3 mg L−1 Picloram). These findings would suggest that at all picloram concentrations tested both varieties of cassava showed a high embryogenic callus production. High frequency on embryogenic callus formation is a key step in cassava regeneration system specifically to support the modern breeding program.
Textile dye decolourisation of yeast isolated from Tuak starter culture
Authors: Setiono S , Sophiano T , Purnomo JS , Victor H , Pinontoan R 
Published: November 2023

Abstract
The textile industry is one of the largest industries in the world and contributes positively to the economic growth of a country. However, the improper disposal of textile dye effluents results in discharging of high concentrations of textile dyes into the environment. Moreover, the inherent stability of textile dyes makes them hard to remove from the environment, which can harm organisms in polluted areas. Biological decolourisation was considered as a strategy to remediate textile dyes. Microbial decolourisation of textile dyes involves using various species of bacteria and fungi from different sources that can degrade or accumulate dye molecules. Although Indonesia is a well–known country with rich biodiversity, research on microbial decolourisation is still limited. Therefore, this study explored the decolourisation potential of microbes from the starter culture of Tuak, a traditional Indonesian alcoholic beverage made from fermented sugar palm or rice. Potential microbes were screened on yeast peptone dextrose solid agar (YPD) supplemented with various textile dyes. Isolates that formed a clear zone around the colony were further examined for decolourisation activity in broth culture media, and the decrease in dye concentration was measured spectrophotometrically. The results showed that two potential yeast isolates could decolourise Congo red and malachite green. Identification of the isolates by observation of colony and cell morphology and biochemical characteristics indicated that the two isolated yeasts belonged to the genera Saccharomyces and(or) Candida.
Biomolecular mycorrhizal diversity in Azadirechta excelsa (Jack) Jacobs roots in habitats with different altitude
Authors: Anwar G , Sipriyadi  , Ketaren C L 
Published: November 2023

Abstract

Azadirechta excelsa is a member of the Meliaceae family with high economic value. This tree is widely planted in South Sumatra, especially in Bengkulu Province. The mutualism symbiosis between several Meliaceae plants and mycorrhiza plants has been studied. However, the diversity of mycorrhiza in the roots of A. excelsa is not well known. We conducted a biomolecular analysis of the roots of this tree species to find out in more detail the types of mycorrhiza associated with the roots and investigated habitat differences in altitude (highland and lowland) that affect mycorrhizal diversity. We used the DNA sequencing method from the roots of A. excelsa and amplified it through PCR using ITS primers forward ITS5.8S and reverse ITS4. We took 14 root samples of E. excelsa; 6 trees from the highland (959 m asl) and eight trees from the lowland (63m asl). The average age of the tree is eight years. The results of the sequencing identification from NCBI using the BLAST method found ten types of mycorrhiza from 4 families associated with A. excelsa roots. They are seven species from the Mycenaceae family (Mycena sp, Filoboletus manipularis, M. amicta, M. pura, M. rosea, M. citrinomarginata, and Favolaschia manipularis), one species from the Psathyrellaceae family (Psathyrella sp), one species from the Tricholomataceae family (Tricholomataceae sp), and one species from the Agaricaceae family (Agaricales sp). All of those types of mycorrhiza belong to the Agaricales order, the Agaricomycetes class from the Basidiomycota phylum. There was no specific type of mycorrhiza that occupied the roots of the tree. Thus, the difference in altitude did not affect the type of mycorrhiza on A. excelsa roots.
Fractional inhibitory concentration index of the allicin and flavonoids combinations against Ganoderma boninense
Authors: Yusup C A , Eris D D , Pradana M G , Mulyatni A S , Widiastuti H 
Published: November 2023

Abstract
Allicin and several flavonoids were known to have antifungal activity against various fungi. The antifungal capability of allicin and flavonoids varies depending on the target fungi; the combination of both compounds was expected to increase its antifungal activity. As a major disease of oil palm, basal stem rot (BSR) caused by Ganoderma boninense always be a top concern of oil palm farmers. This study aimed to evaluate the effect of combining allicin with hesperidin and rutin on antifungal activity against G. boninense through the fractional inhibitory concentration (FIC) index. The FIC index was determined using a checkerboard assay with media poisoning methods. The allicin concentration tested was 0.1%, 0.2%, 0.4%, and 0.8%, while the hesperidin and rutin concentrations were 1.25%, 2.5%, 5%, and 10%. All combinations were done in triplicate and incubated for twelve days. The result showed that a combination of allicin with hesperidin or rutin was the additive interaction that lowers the concentration of each compound with similar inhibition rate of G. boninense in vitro. Combining allicin with rutin was considered better than hesperidin due to the higher inhibition rate of G. boninense. The allicin-rutin combination has a lower concentration with a 100% inhibition rate of G. boninense in vitro.
Determination of Marker Compounds of Senna alata from different location in East Kalimantan, Indonesia
Authors: Salam D M , Laksmita A N , Fernandes A , Maharani R , Saputro S H 
Published: November 2023

Abstract

Senna alata is a plant used for medical purposes, and its leaves have an extended history of use as a traditional herbal medicine in Indonesia. S. alata is known to contain some components of biologically active compounds and also secondary metabolites. In fact, S. alata can grow well in various locations in Indonesia, location differences can lead to differences in compound content due to differences in environmental conditions such as soil, rainfall, light intensity, and humidity. Therefore, this study aimed to analyze marker compounds of S. alata plants origin from three different location in East Kalimantan, i.e Samarinda, Samboja, and Berau. This research was conducted to estimate the compounds contained in extracts by using GC-MS analysis, and to discover relationships between different variables by using Principal Component Analysis (PCA). There are variances in the yield of secondary metabolites according on where Senna alata is grown, specifically in the riverside Nyapa Indah region and the plains of Samarinda and Samboja. Based on GC-MS test results, Phytol was the main compound in S.alata in two areas, i.e. Samarinda and Samboja. Meanwhile, in Berau showed that 1,2-Benzenedicarboxylic acid, and mono (2-ethylhexyl) ester were the main components. However, S. alata leaf extracts could be used as a good quality raw material for pharmaceutical industries, such as a laxative agent.
Alcohol concentration from fermentation and distillation of Palm sap (Arenga pinnata) in North Halmahera, Indonesia
Authors: Anwar Y , Puspitasari C , Fatriasari W 
Published: November 2023

Abstract
Palm plants (Arenga pinnata) are multifunctional plants because almost all parts of this plant can be used for the needs of the local community. The main products of tapping palm sap can be used as sugar, drinks, vinegar, and alcohol. This study aims to identify the alcohol concentration of fermentation and distillation of palm sap in North Halmahera, Indonesia. The method of making alcoholic beverages from the tapping of palm sap is carried out traditionally by farmers of Nort Halmahera, with the first stage, namely palm sap fermented for three days. The second stage is the distillation process. Then the distillation results are measured alcohol concentration using an alcohol detection device, namely an Alcoholmeter. The results, showed that the alcohol concentration produced after the fermentation process for 3 days was 3%. The alcohol concentration produced in the first distillation is 70%, the second distillation alcohol concentration is 45%, and the third distillation alcohol concentration is 31%. It is found that it takes 11 hours to get the level of alcohol that can be consumed.