bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetics—An Epicenter of Gene Regulation: Histones and Histone‐Modifying Enzymes
Authors: Biel Markus , Wascholowski Veit , Giannis Athanassios 
Published: May 2005

AbstractThe treatment of cancer through the development of new therapies is one of the most important challenges of our time. The decoding of the human genome has yielded important insights into the molecular basis of physical disorders, and in most cases a connection between failures in specific genes and the resulting clinical symptoms can be made. The modulation of epigenetic mechanisms enables, by definition, the alteration of cellular phenotype without altering the genotype. The information content of a single gene can be crucial or harmful, but the prerequisite for a cellular effect is active gene transcription. To this end, epigenetic mechanisms play a very important role, and the transcription of a given gene is directly influenced by the modification pattern of the surrounding histone proteins as well as the methylation pattern of the DNA. These processes are effected by different enzymes which can be directly influenced through the development of specific modulators. Of course, all genetic information is written as a four‐character code in DNA. However, epigenetics describes the art of reading between the lines.
Pharmacokinetics and tissue distribution of d‐alpha‐tocopheryl succinate formulations following intravenous administration in the rat
Authors: Teng Xiao Wei , Davies Neal M. , Fukuda Chie , Good Renee L. , Fariss Marc W. 
Published: May 2005

AbstractUnlike d‐alpha tocopherol (T), d‐alpha tocopheryl succinate (TS) has the unique ability to selectively kill tumor cells while protecting normal tissue from toxic oxidative stress. The pharmacokinetics of TS and the serum and tissue disposition of TS were studied in male Sprague‐Dawley rats to delineate formulation dependent disposition between TS administered as the Tris salt (TS‐T) (a liposomal formulation) or as the free acid (TS‐FA) dissolved in polyethylene glycol (PEG) 400. The pharmacokinetics of TS was studied after single intravenous (i.v.) equimolar doses of 124 mg/kg TS‐T and 100 mg/kg of TS‐FA. Serial blood samples were collected via a catheter inserted into the right jugular vein and serum samples were analysed for TS and T levels using a reverse phase HPLC method. Terminal tissue samples were also collected at 24 and 48 h. After an acute i.v. dose of TS‐T, serum AUC, t1/2, Cl and Vd of TS were 2601.0±351.7 µg h/ml, 9.98±1.02 h, 0.049±0. 0073 l/h/kg and 0.7±0.14 l/kg (mean±SD), respectively. The acute i.v. administration of TS‐FA (PEG formulation) yielded results similar to those observed for TS‐T, with a serum AUC, t1/2, Cl and Vd of 2553.3±166.4 µg h/ml, 9.83±0.86 h, 0.039±0.0027 l/h/kg and 0.56±0.09 l/kg (mean±SD), respectively. Distribution into tissues and a low Cl was apparent, with the highest concentrations of TS in the liver and lung, regardless of formulation. As expected, baseline endogenous concentrations of T were present in both groups, with a net increase in T levels, occurring as TS was hydrolysed to T, which slowly peaked in serum between 7–8 h post‐dose. Intravenous TS administration, regardless of formulation, also resulted in significant T accumulation in all tissues examined, which was especially abundant in the liver and lung. Likewise, there was a lack of significant effect of formulation on the pharmacokinetics and tissue distribution of TS. The only significant formulation difference was a small but significant increase in serum T and liver T levels in the TS in PEG formulation group. These results indicate that TS may be especially useful for the targeted delivery of T and TS to the lung and liver for anti‐oxidant and anti‐cancer activity. Copyright © 2005 John Wiley & Sons, Ltd.
Stent‐based delivery of ABT‐578 via a phosphorylcholine surface coating reduces neointimal formation in the porcine coronary model
Authors: Collingwood Robin , Gibson Lori , Sedlik Souad , Virmani Renu , Carter Andrew J. 
Published: May 2005

AbstractStent‐based delivery of the antiproliferative and immunosuppressive macrocyclic lactone sirolimus reduces neointimal formation and restenosis by cytostatic inhibition of vascular smooth muscle cell proliferation. The objective of this study was to determine the feasibility and efficacy of stent‐based delivery of ABT‐578, a structurally unique macrocyclic lactone. Stainless steel balloon‐expandable stents were coated with thin layer of phosphorylcholine (PC) or PC with ABT‐578 (10 μg/mm). Fifteen juvenile domestic pigs underwent placement of oversized bare metal (n = 15), PC (n = 8), and PC with ABT‐578 (n = 9) stents in the coronary arteries. At 28 days, histology demonstrated similar mean injury scores for the control, PC‐, and ABT‐578‐coated stents. The mean neointimal area (mm2) was significantly reduced for ABT‐578 (1.70 ± 0.47) as compared with PC (2.82 ± 1.24) and control (2.89 ± 1.91) stents (P ≤ 0.05). The 40% reduction in neointimal area resulted in significantly less mean percent diameter stenosis for ABT‐578 (19.4% ± 4.0%) as compared with PC (30.3 ± 12.1 %) and control (29.4% ± 15.5%) stents (P ≤ 0.03). Twelve of the 45 bare metal stent cross‐sections (26.7%) exhibited a giant cell reaction, while none of the sections from the ABT‐578‐eluting stents had a giant cell reaction (P = 0.004). Stent‐based delivery of ABT‐578 via a PC surface coating inhibits neointimal formation at 28 days in the porcine coronary model. Further study is necessary to determine the dose‐response and long‐term effects ABT‐578‐eluting stents in the porcine coronary model.© 2005 Wiley‐Liss, Inc.
Effect of stress on viral–bacterial synergy in bovine respiratory disease: novel mechanisms to regulate inflammation
Authors: Hodgson P. D. , Aich P. , Manuja A. , Hokamp K. , Roche F. M. , Brinkman F. S. L. , Potter A. , Babiuk L. A. , Griebel P. J. 
Published: May 2005

AbstractThe severity of bovine respiratory infections has been linked to a variety of factors, including environmental and nutritional changes, transportation, and social reorganization of weaned calves. Fatal respiratory infections, however, usually occur when a primary viral infection compromises host defences and enhances the severity of a secondary bacterial infection. This viral–bacterial synergy can occur by a number of different mechanisms and disease challenge models have been developed to analyse host responses during these respiratory infections. A primary bovine herpesvirus‐1 (BHV‐1) respiratory infection followed by a secondary challenge with Mannheimia haemolytica results in fatal bovine respiratory disease (BRD) and host responses to these two pathogens have been studied extensively. We used this disease model to demonstrate that stress significantly altered the viral–bacterial synergy resulting in fatal BRD. Functional genomic analysis revealed that BHV‐1 infection enhanced toll‐like receptors (TLR) expression and increased pro‐inflammatory responses which contribute to the severity of a Mannheimia haemolytica infection. TLRs play a critical role in detecting bacterial infections and inducing pro‐inflammatory responses. It is difficult to understand, however, how stress‐induced corticosteroids could enhance this form of viral–bacterial synergy. Nuclear translocation of the glucocorticoid receptor activates cell signalling pathways which inhibit both TLR signalling and pro‐inflammatory responses. The apparent conundrum between stress‐induced corticosteroids and enhanced BRD susceptibility is discussed in terms of present data and previous investigations of stress and respiratory disease. Copyright © 2005 John Wiley & Sons, Ltd.
Xenogenomics: genomic bioprospecting in indigenous and exotic plants through EST discovery, cDNA microarray‐based expression profiling and functional genomics
Authors: John Ulrik P. , Spangenberg German C. 
Published: May 2005

AbstractTo date, the overwhelming majority of genomics programs in plants have been directed at model or crop plant species, meaning that very little of the naturally occurring sequence diversity found in plants is available for characterization and exploitation. In contrast, ‘xenogenomics’ refers to the discovery and functional analysis of novel genes and alleles from indigenous and exotic species, permitting bioprospecting of biodiversity using high‐throughput genomics experimental approaches. Such a program has been initiated to bioprospect for genetic determinants of abiotic stress tolerance in indigenous Australian flora and native Antarctic plants. Uniquely adapted Poaceae and Fabaceae species with enhanced tolerance to salt, drought, elevated soil aluminium concentration, and freezing stress have been identified, based primarily on their eco‐physiology, and have been subjected to structural and functional genomics analyses. For each species, EST collections have been derived from plants subjected to appropriate abiotic stresses. Transcript profiling with spotted unigene cDNA micro‐arrays has been used to identify genes that are transcriptionally modulated in response to abiotic stress. Candidate genes identified on the basis of sequence annotation or transcript profiling have been assayed in planta and other in vivo systems for their capacity to confer novel phenotypes. Comparative genomics analysis of novel genes and alleles identified in the xenogenomics target plant species has subsequently been undertaken with reference to key model and crop plants. Copyright © 2005 John Wiley & Sons, Ltd.
A phylogenomic study of the genus Alphavirus employing whole genome comparison
Authors: Luers Aimée J. , Adams Sandra D. , Smalley John V. , Campanella James J. 
Published: May 2005

AbstractThe phylogenetics of the genus Alphavirus have historically been characterized using partial gene, single gene or partial proteomic data. We have mined cDNA and amino acid sequences from GenBank for all fully sequenced and some partially sequenced alphaviruses and generated phylogenomic analyses of the genus Alphavirus genus, employing capsid encoding structural regions, non‐structural coding regions and complete viral genomes. Our studies support the presence of the previously reported recombination event that produced the Western Equine Encephalitis clade, and confirm many of the patterns of geographic radiation and divergence of the multiple species. Our data suggest that the Salmon Pancreatic Disease Virus and Sleeping Disease Virus are sufficiently divergent to form a separate clade from the other alphaviruses. Also, unlike previously reported studies employing limited sequence data for correlation of phylogeny, our results indicate that the Barmah Forest Virus and Middelburg Virus appear to be members of the Semliki Forest clade. Additionally, our analysis indicates that the Southern Elephant Seal Virus is part of the Semliki Forest clade, although still phylogenetically distant from all known members of the genus Alphavirus. Finally, we demonstrate that the whole Rubella viral genome provides an ideal outgroup for phylogenomic studies of the genus Alphavirus. Copyright © 2005 John Wiley & Sons, Ltd.
Rhodium‐Catalyzed Dehydrocoupling of Fluorinated Phosphine–Borane Adducts: Synthesis, Characterization, and Properties of Cyclic and Polymeric Phosphinoboranes with Electron‐Withdrawing Substituents at Phosphorus
Authors: Clark Timothy J. , Rodezno José M. , Clendenning Scott B. , Aouba Stephane , Brodersen Peter M. , Lough Alan J. , Ruda Harry E. , Manners Ian 
Published: May 2005

AbstractThe dehydrocoupling of the fluorinated secondary phosphine–borane adduct R2PH⋅BH3 (R = p‐CF3C6H4) at 60 °C is catalyzed by the rhodium complex [{Rh(μ‐Cl)(1,5‐cod)}2] to give the four‐membered chain R2PH‐BH2‐R2P‐BH3. A mixture of the cyclic trimer [R2P‐BH2]3 and tetramer [R2P‐BH2]4 was obtained from the same reaction at a more elevated temperature of 100 °C. The analogous rhodium‐catalyzed dehydrocoupling of the primary phosphine–borane adduct RPH2⋅BH3 at 60 °C gave the high molecular weight polyphosphinoborane polymer [RPH‐BH2]n (Mw = 56 170, PDI = 1.67). The molecular weight was investigated by gel permeation chromatography and the compound characterized by multinuclear NMR spectroscopy. Interestingly, the electron‐withdrawing fluorinated aryl substituents have an important influence on the reactivity as the dehydrocoupling process occurred efficiently at the mildest temperatures observed for phosphine–borane adducts to date. Thin films of polymeric [RPH‐BH2]n (R = p‐CF3C6H4) have also been shown to function as effective negative‐tone resists towards electron beam (e‐beam) lithography (EBL). The resultant patterned bars were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) and time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS).
Cation Distribution and Phase Transformations in LiMn2‐yTiyO4 (0.2 ≤ y ≤ 1.5) Solid Solutions.
Authors: Petrov Kostadin , Rojas Rosa M. , Alonso Pablo J. , Amarilla Jose M. , Lazarraga Monica G. , Rojo Jose M. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Polymorphism of Anhydrous Cadmium Iodate Structure of ε‐Cd(IO3)2.
Authors: Bentria Bachir , Benbertal Djamal , Hebboul Zoulikha , Bagieu‐Beucher Muriel , Mosset Alain 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Solubility, Complex Formation, and Redox Reactions in the Tl2O3—HCN/CN‐—H2O System. Crystal Structures of the Cyano Compounds Tl(CN)3×H2O, Na[Tl(CN)4]×3H2O, K[Tl(CN)4], and TlI[TlIII(CN)4] and of TlI2C2O4.
Authors: Nagy Peter , Fischer Andreas , Glaser Julius , Ilyukhin Andrey , Maliarik Mikhail , Toth Imre 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Hydrothermal Syntheses and Crystal Structures of Two Novel, Hybrid Materials Based on Secondary Transition‐Metal‐Incorporated Polyoxovanadate Cluster Backbones: [Cd(dien)2]2 [(dien)CdAs8V13O41(H2O)] ×4H2O and [Cd(en)2]2 [(en)2Cd2As8V12O40].
Authors: Zheng Shou‐Tian , Zhang Jie , Yang Guo‐Yu 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Cyclic Polyvanadates Incorporating Template Transition Metal Cationic Species: Synthesis and Structures of Hexavanadate [PdV6O18]4‐, Octavanadate [Cu2V8O24]4‐, and Decavanadate [Ni4V10O30(OH)2 (H2O)6]4‐.
Authors: Kurata Taisei , Uehara Akira , Hayashi Yoshihito , Isobe Kiyoshi 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Control of Nanometer‐Scale Tunnel Sizes of Porous Manganese Oxide Octahedral Molecular Sieve Nanomaterials.
Authors: Shen Xiong‐Fei , Ding Yun‐Shuang , Liu Jia , Cai Jun , Laubernds Kate , Zerger Richard P. , Vasiliev Alexandre , Aindow Mark , Suib Steven L. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Copper(II) Triflate Immobilized in [bmin]BF4 Ionic Liquid: An Efficient Reaction Medium for Michael Addition of β‐Ketoesters to Acceptor‐Activated Alkenes.
Authors: Yadav J. S. , Reddy B. V. S. , Baishya Gakul , Narsaiah A. Venkat 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Dehydrohalogenation of Substituted Diethyl Halocyclopropane‐1,1‐dicarboxylates.
Authors: Boitsov V. M. , Kostikov R. R. , Molchanov A. P. , Stepakov A. V. , Baird M. S. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Structure of the Reduction Products of N‐(m‐Tolyl)‐exo‐2,3‐epoxybicyclo [2.2.1]heptane‐endo‐5,endo‐6‐dicarboximide with Lithium Tetrahydridoaluminate.
Authors: Kas'yan L. I. , Krishchik O. V. , Kas'yan A. O. , Tarabara I. N. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reactions of N‐ and C‐Alkenylanilines. Part 6. Synthesis of 6‐Methyl‐2‐[(E or Z)‐1‐propenyl]anilines and the Corresponding Anilides and Their Reaction with Bromine.
Authors: Afon'kin I. S. , Sotnikov A. M. , Gataullin R. R. , Spirikhin L. V. , Abdrakhmanov I. B. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Specific Character of the Pechmann Reaction of 4,8‐Di‐tert‐butyl‐5‐hydroxy‐2‐(4‐methoxyphenyl)‐2H‐naphtho[1,8‐bc]furan with Ethyl Acetoacetate.
Authors: Tyurin R. V. , Antonov A. N. , Minyaeva L. G. , Mezheritskii V. V. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Asymmetric Synthesis of Heterocyclic β‐Aminosulfones via Nucleophilic 1,2‐Addition of 2‐Lithio‐benzo[b]thiophene to Aldehydes‐SAMP‐Hydrazones.
Authors: Enders Dieter , Del Signore Giuseppe 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Palladium(0)‐Catalyzed Amino‐Heck Reaction of γ,δ‐Unsaturated Ketone N,N,N‐Trimethylhydrazonium Salts.
Authors: Kitamura Mitsuru , Yanagisawa Hideyuki , Yamane Motoki , Narasaka Koichi 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Effective scCO2‐Ionic Liquid Reaction System Based on Symmetric Aliphatic Ammonium Salts for the Rapid CO2 Fixation with Aziridine to 2‐Oxazolidinone.
Authors: Kawanami Hajime , Matsumoto Hajime , Ikushima Yutaka 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A New [2 + 2] Photodimerization of 5‐Chloro‐ and 5‐Methyl‐2‐pyridone in Their Inclusion Complexes with 1,1′‐Biphenyl‐2,2′‐dicarboxylic Acid as a Model for DNA Damage by Photodimerization of Its Thymine Component.
Authors: Hirano Shinya , Toyota Shinji , Toda Fumio 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Base‐Induced Generation of Aryl(1,2,3‐triazol‐1‐yl)carbenes from 1‐[(N‐Phenylsulfonyl)benzohydrazonoyl]‐1,2,3‐triazoles and Their Ring Enlargement to 3‐Aryl‐1,2,4‐triazines.
Authors: Tanaka Yuumo , Oda Shuji , Ito Suketaka , Kakehi Akikazu 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Microwave‐Assisted Synthesis of Some New Isoxazolyl‐[1,3,5]‐triazinan‐2‐ones and Isoxazolyl‐[1,3,5]‐oxadiazinan‐4‐ones in Dry Media.
Authors: Rajanarendar E. , Afzal Md. , Ramu K. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A New Method for the Lactonization of ω‐Hydroxy Carboxylic Acids with Di‐2‐thienyl Carbonate by the Promotion of DMAP and Iodine.
Authors: Oohashi Yoshiaki , Fukumoto Kentarou , Mukaiyama Teruaki 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Regioselectivity in Diels—Alder Reactions of Thiazolo[3,2‐d][1,4,2]diazaphospholes and Related Compounds.
Authors: Bansal Raj K. , Karaghiosoff Konstantin , Gupta Neelima , Kabra Vijaya , Mahnot Ruchi , Sharma Dinesh C. , Munjal Renu , Kumawat Surendra K. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Bis(ethylenedioxy)‐1,4,5,8‐tetraselenanaphthalene: The First Example of Tetraselenanaphthalene.
Authors: Watanabe Ryoji , Miyake Yoshihiro , Kuwatani Yoshiyuki , Iyoda Masahiko 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Cheiloclines A—I. First Examples of Octacyclic Sesquiterpene‐Triterpene Hetero‐Diels—Alder Adducts.
Authors: Mesa‐Siverio Dulce , Chavez Haydee , Estevez‐Braun Ana , Ravelo Angel G. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthetic Studies of the Cyclic Depsipeptides Bearing the 3‐Amino‐6‐hydroxy‐2‐piperidone (Ahp) Unit. Total Synthesis of the Proposed Structure of Micropeptin T‐20.
Authors: Yokokawa Fumiaki , Inaizumi Akiko , Shioiri Takayuki 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Ziziphine N, O, P and Q, New Antiplasmodial Cyclopeptide Alkaloids from Ziziphus oenoplia var. brunoniana.
Authors: Suksamrarn Sunit , Suwannapoch Narisara , Aunchai Natthachai , Kuno Mayuso , Ratananukul Piniti , Haritakun Rachada , Jansakul Chawewan , Ruchirawat Somsak 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Chemistry of Indoles Carrying a Basic Function. Part 9. Unexpected Cyclizations of Diketones Derived from Uhle′s Ketone.
Authors: Moldvai Istvan , Gacs‐Baitz Eszter , Temesvari‐Major Eszter , Incze Maria , Poppe Laszlo , Szantay Csaba 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
1‐Butyl‐3‐methylimidazolium 3,5‐Dinitro‐1,2,4‐triazolate: A Novel Ionic Liquid Containing a Rigid, Planar Energetic Anion
Authors: Katritzky Alan R. , Singh Shailendra , Kirichenko Kostyantyn , Holbrey John D. , Smiglak Marcin , Reichert W. Matthew , Rogers Robin D. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
C—H Bond Activation by Iridium and Rhodium Complexes: Catalytic Hydrogen—Deuterium Exchange and C—C Bond‐Forming Reactions
Authors: Klei Steven R. , Tan Kian L. , Golden Jeffrey T. , Yung Cathleen M. , Thalji Reema K. , Ahrendt Kateri A. , Ellman Jonathan A. , Tilley T. Don , Bergman Robert G. 
Published: May 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Effects of weekly paclitaxel or paclitaxel plus carboplatin on functionality and symptoms of geriatric patients with cancer as measured by a brief geriatric oncology module
Authors: Presant Cary A. , Thompson Erin , LeBerthon Brian , Vergara Lori , McGowen Priscilla 
Published: May 2005

AbstractBACKGROUNDTo measure functionality, symptoms, and quality of life in elderly patients, a geriatric oncology module (GOM) was developed that could be rapidly and effectively administered in community oncology practices.METHODSThe GOM was validated by semi structured patient interviews. The authors tested 26 patients receiving chemotherapy with paclitaxel or paclitaxel plus carboplatin to determine the effect of drug treatment on functionality, symptoms, and quality of life (QOL).RESULTSChemotherapy stabilized or improved functionality, symptoms, and QOL in 75%, 86%, and 76% of patients, respectively. This GOM was easily implemented in a busy community oncology practice.CONCLUSIONSWeekly paclitaxel and paclitaxel plus carboplatin produced measurable palliation in geriatric oncology patients. This GOM may be useful in the assessment of specific chemotherapeutic interventions in elderly patients with cancer in community oncology practices. Cancer 2005. © 2005 American Cancer Society.
Clinical and immunohistologic typing of salivary duct carcinoma
Authors: Jaehne Michael , Roeser Kerstin , Jaekel Thorsten , Schepers Jan David , Albert Natalie , Löning Thomas 
Published: May 2005

AbstractBACKGROUNDDue to the low incidence of salivary duct carcinoma (SDC), only limited data in regard to the biologic behavior of this tumor and its immunohistochemical characteristics are available. The authors analyzed the clinical, molecular, and genetic profile of SDC and identified prognostic factors.METHODSThe follow‐up of 50 patients with SDC was obtained and paraffin‐embedded tumor samples were examined immunohistochemically. In all samples, the expression of Ki‐67, HER‐2, and the oncoproteins p16 and p53 was examined immunohistochemically, followed by a mutation analysis of p16 and p53. The survival rate was calculated by the Kaplan–Meier method and prognostic variables were analyzed with the log‐rank test.RESULTSSDC predominantly effected male patients (66%) in their 7th decade of life. SDC mainly occurred in the parotid gland (78%; submandibular gland, 12%; minor salivary glands, 10%). Approximately two‐thirds of the patients (33 of 50) presented with a T3/T4 tumor. In 28 patients (56%), cervical lymph node metastasis was present at the time of diagnosis. Local disease recurrence was observed in 48% of patients an average of 17.4 months after initial treatment. Distant disease metastasis developed in 48% of patients an average of 29 months after initial treatment. The average overall survival period was 56.2 months. In the current study, 20.6% of the probes with positive HER‐2/neu expression were (+++) positive. p53 was expressed in 83.9% of the tumor samples. In 11.8% of the tumor samples, there was a lack of p16 expression.CONCLUSIONSMutations of the p53 gene were more frequent in tumor samples with (++) and (+++) immunoreactivity and mainly affected exons 7 and 8. A mutation of the p16 gene was only found in 1 tumor sample. Expression of HER‐2/neu and p53 was statistically linked (P < 0.05) to early local disease recurrence, distant disease metastasis, and survival rates. Cancer 2005. © 2005 American Cancer Society.