bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omics analysis reveals distinct gene regulatory mechanisms between primary and organoid-derived human hepatocytes
Authors: Wu Haoyu , Yang Annie S. P. , Stelloo Suzan , Roos Floris J. M. , te Morsche René H. M. , Verkerk Anne H. , Luna-Velez Maria V. , Wingens Laura , de Wilt Johannes H. W. , Sauerwein Robert W. , Mulder Klaas W. , van Heeringen Simon J. , Verstegen Monique M. A. , van der Laan Luc J. W. , Marks Hendrik , Bártfai Richárd 
Published: January 2025

ABSTRACT
Hepatic organoid cultures are a powerful model to study liver development and diseases in vitro. However, hepatocyte-like cells differentiated from these organoids remain immature compared to primary human hepatocytes (PHHs), which are the benchmark in the field. Here, we applied integrative single-cell transcriptome and chromatin accessibility analysis to reveal gene regulatory mechanisms underlying these differences. We found that, in mature human hepatocytes, activator protein 1 (AP-1) factors co-occupy regulatory regions with hepatocyte-specific transcription factors, including HNF4A, suggesting their potential cooperation in governing hepatic gene expression. Comparative analysis identified distinct transcription factor sets that are specifically active in either PHHs or intrahepatic cholangiocyte organoid (ICO)-derived human hepatocytes. ELF3 was one of the factors uniquely expressed in ICO-derived hepatocytes, and its expression negatively correlated with hepatic marker gene expression. Functional analysis further revealed that ELF3 depletion increased the expression of key hepatic markers in ICO-derived hepatocytes. Our integrative analysis provides insights into the transcriptional regulatory networks of PHHs and hepatic organoids, thereby informing future strategies for developing improved hepatic models.
THE DEMAND FOR VIDEO CONTENT AMONG STUDENT YOUTH IN MODERN SOCIETY
Authors: Baranova Ekaterina V. , Guro-Frolova Yulia R. , Sokolova Elena G. 
Published: January 2025

Background. The article is devoted the topical issue of the impact of digital transformation processes in all spheres of human activity on the choice of means of personal and professional formation and development among student youth. The issue study was conducted by the authors in the form of a sociological experiment on the basis of the Volga State University of Water Transport in Nizhny Novgorod (hereinafter referred to as the VSUWT). The subject of the study is video content produced in Russia and abroad.
Purpose. The authors aim to study the relevance and number of viewing Russian and foreign video content in the Internet among the students of VSUWT.
Methodology. The research is based on statistical and mathematical methods of data collecting and processing. A sociological experiment without observing subsequent changes is an empirical, descriptive study conducted through specially designed electronic survey forms in the Internet on the Yandex platform, as well as oral standardized interviewing.
Results. The results of the study are that the authors reveal a high demand for video content in the Internet among the younger generation of the considered socio-demographic group in the absence of a specific priority regarding its origin country. Statistical analysis of the data obtained showed that the main goals of watching video content are both entertainment and vocational education, contributing to the formation of future specialists.
Practical implications. The results of the study can be applied in solving the problem of sociological assessment of digital transformation success on the platform of transport and other specialized universities, as well as in the process of improving the digital literacy level in modern society, in the search for effective ways of socializing a person in the information-boosted environment.
Moreover, the demand for video content is a promising guideline in optimizing the higher professional education system. The popularity of digitalized information among students can help to increase the motivation of young people to self-education.
SLEEP AS A FOUNDATION OF SOCIAL HEALTH: A STUDY OF ADOLESCENT HEALTH AND SLEEP QUALITY
Authors: Ledyankina Ksenia Yu. 
Published: January 2025

Objective. This study aims to analyze the quality of sleep among adolescents aged 15-18 years and to identify factors contributing to sleep deprivation. The main goal of the work is to assess the impact of sleep patterns on the health and emotional well-being of adolescents, as well as to develop recommendations for improving sleep quality.
Methods and methodology. During the study, a survey was conducted among school students to identify their sleep habits, level of awareness regarding the rules of healthy sleep, and factors contributing to disruptions in their sleep patterns. The analysis of the collected data allowed for the identification of the main causes of insufficient sleep, as well as the conduction of a correlational analysis of the impact of various factors on sleep quality. In addition, the research included an analysis of the existing scientific literature on the topic.
Results. The results of the study showed that more than 70% of respondents do not adhere to optimal sleep conditions, leading to chronic sleep deprivation, decreased concentration, impaired memory, and negative effects on emotional well-being. It was found that high academic workloads and participation in additional courses significantly disrupt sleep patterns. Furthermore, a lack of knowledge about sleep hygiene and its importance for health contributes to the deepening of the problem. In this regard, recommendations have been developed regarding optimal sleep duration, limiting screen time usage, and creating a comfortable environment for rest.
Application of results. The results of the study can be applied in educational institutions to create programs aimed at educating adolescents about the importance of adequate sleep. The recommendations can be used as tools for working with parents and educators to foster a conscious approach to sleep patterns and a healthy lifestyle.
ENSEMBLE MUSIC AS EDUCATIONAL AND COMMUNICATIVE RESOURCE. ONLINE SEMINAR REVIEW
Authors: Boiko Valentina L. , Mitasova Svetlana A. , Chaikin Sergey G. 
Published: January 2025

Music has always been a magnet for the greatest minds of mankind considering it as a cultural phenomenon, as manifestation of Divinity, and as a specific art form. Philosophic studies of music nature show that origins of spiritual culture can be seen as deep as in the earliest civilization periods.
Significance of music is determined by its contents, its narrative method and peculiarities of the musical language proper. Nowadays music is the factor that impacts formation of personality, its interaction with the society and its communication with the environment.
Topicality of ensemble music, its educational and communicative qualities are conditioned by ever growing role of music as an art form in general, and ensemble music as its significant part in the constantly spreading information sphere.
The purpose of the topic under discussion is to show the complex and many-sided character of music ensemble, starting from its philosophic basics and going to performance analysis of specific musical pieces.
The authors’ tasks are defined by the discussion purpose and are achieved by presentation of variety of formative and communicative forms, methods and techniques in the work with young musicians in ensemble class.
Results. Accumulation and summarizing theoretical and practical educational and tutorial experience with students in the process of moulding young musician professional and personal formation.
Exploring how the uptake of childhood immunisations can be improved in England: A systematic review
Authors: Ames Sophie , Kelly Gill 
Published: January 2025

A review of best practice in child immunisation was undertaken to help drive uptake Background Childhood immunisations protect communities against preventable and infectious diseases. Coverage for childhood immunisations in the UK is declining and coverage for the MMR vaccine stands at 85.5%, significantly below the target set by the Department of Health and Social Care to achieve herd immunity and prevent sustained disease transmission. Methods Three medical databases were searched using PICO, supported by the application of inclusion and exclusion criteria and critical appraisal. Results Reminder and recall activities are identified as a cost-effective method for improving childhood immunisation uptake but targeted, individualised and family-centred interventions should be utilised to promote access to childhood immunisations for underserved populations. Vaccination data should remain up to date and a child's vaccination status should be reviewed and meaningfully discussed at every healthcare contact and staff involved in immunisation should have access to appropriate education, training and support. Recommendations Understanding how to provide services and improve vaccine coverage for patients who frequently are not brought is identified as an opportunity for further research, as well as analysing the role of public health teams in identifying local inequalities and supporting GP practices to design and deliver bespoke interventions for their community. It is recommended that communication emphasises the safety and effectiveness of vaccines, while highlighting a child's susceptibility or risk to developing complications. Further research is needed to evaluate how best to communicate this to ensure that the information provided is clear and factual and comprehensible to all parents and carers.
Influenza, vaccines and new developments
Authors: Heffernan Catherine 
Published: January 2025

Catherine Heffernan provides an update on influenza vaccination Vaccines are the first step in prevention of many infectious disease such as influenzae and have has dramatically reduced the burden of infectious disease ( van Panhuis et al, 2013 ). Their aim is to induce an immune response to the targeted pathogen without developing the disease. Vaccines work by initiating an innate immune response which is the body's first line of defence against invading pathogens. The Influenza virus is a challenge for global immunisation programmes as the virus can change and adapt creating a need for an annual vaccine and continuous monitoring of the effectiveness of these interventions
Do global health networks facilitate innovation, learning and sharing? A qualitative analysis of the Quality-of-Care Network in Bangladesh, Ethiopia, Malawi, and Uganda
Authors: Mwandira Kondwani , Lemma Seblewengel , Dube Albert , Akter Kohenour , Tufa Asebe Amenu , Kyamulabi Agnes , Seruwagi Gloria , Nakidde Catherine , Mwaba Kasonde , Djellouli Nehla , Makwenda Charles , Colbourn Tim , Shawar Yusra Ribhi 
Published: January 2025

The Quality-of-Care Network (QCN), launched by WHO and partners, links global and national actors across several countries to improve maternal and newborn health. We conducted a prospective qualitative study to examine how QCN in Bangladesh, Ethiopia, Malawi and Uganda facilitated learning, sharing, and innovation within and between network countries. We conducted 227 key informant interviews with QCN actors at global, national, and facility levels iteratively in two to four rounds from June 2019 to March 2022. We also reviewed all accessible QCN documents. Drawing on knowledge sharing theory, we thematically analysed the qualitative data according to three themes: sharing, learning, and innovations. Sharing and learning were evident through virtual and in-person platforms including conferences and webinars, held on online resource libraries such as the QCN website. This provides access to strategies and approaches shared by countries and actors. Locally, there was a strengthening of learning collaborative meetings, coaching, and mentorship. Regular meetings, such as stakeholder coordination meetings and learning collaborative sessions, provided opportunity for stakeholders to strategize, share and learn maternal and child health approaches. The network also promoted coordination among stakeholders. Common sharing and learning approaches, such as learning collaborative sessions, were evident across QCN countries. However, innovation was not as apparent across countries. While there were some exceptions, such as the development and adoption of innovative software applications aimed at boosting the capacity of service providers in network countries, these were limited. Most innovation approaches were similar to pre-existing maternal health approaches, adopted from an era preceding the QCN. Nevertheless, there was evidence that QCN improved their functionality. We provide evidence of how learning, sharing, and innovation among and within countries can be fostered for improving maternal and child health; and limitations. This understanding may help country efforts to achieve targets for ending preventable maternal and neonatal deaths.
Antibody response to Plasmodium vivax in the context of Epstein-Barr virus (EBV) co-infection: A 14-year follow-up study in the Amazon rainforest
Authors: Guimarães Luiz F. F. , Rodrigues Bárbara A. , Dias Michelle H. F. , Barcelos Matheus G. , Nascimento Maria F. A. , Moreira-Nascimento Sâmick L. , Afonso Sofia L. , Abreu Barbara G. S. , Middeldorp Jaap M. , Ntumngia Francis B. , Adams John H. , Fabbri Camila , Lopes Stefanie , Fernandes Cor J. F. , Kano Flora S. , Carvalho Luzia H. 
Published: January 2025

Background
To develop an effective vaccine against Plasmodium vivax, the most widely dispersed human malaria parasite, it is critical to understand how coinfections with other pathogens could impact malaria-specific immune response. A recent conceptual study proposed that Epstein-Barr virus (EBV), a highly prevalent human herpesvirus that establishes lifelong persistent infection, may influence P. vivax antibody responses. Here, it was investigated whether EBV could impact the longevity of humoral immune response to P. vivax.

Methodology/principal findings
A 14-year follow-up study was carried out among long-term P. vivax-exposed Amazonian individuals (272, median age 35 years), and included 9 cross-sectional surveys at periods of high and low malaria transmission. The experimental approach focused on monitoring antibodies to the major blood-stage P. vivax vaccine candidate, the Duffy binding protein region II (DBPII-Sal1), including a novel engineered DBPII-based vaccine targeting conserved epitopes (DEKnull-2). In parallel, the status of EBV infection was determined over time by the detection of circulating EBV DNA (EBV-DNAemia) and EBV-specific antibodies to lytic (VCAp18) or latent (EBNA1) antigens. Regardless of the malaria transmission period, the results demonstrated that one or multiple episodes of EBV-DNAemia did not influence the longevity of DBPII immune responses to both strain-specific (Sal-1) or strain-transcending (DEKnull-2) antibodies. Also, the average time in which DBPII-responders lost their antibodies was unrelated to the EBV serostatus. Considering all malaria cases detected during the study, there was a predominance of P. vivax mono-infection (76%), with a positive correlation between malaria infection and EBV-DNAemia.

Conclusions/significance
In an immunocompetent P. vivax-exposed adult population neither sporadic episodes of EBV-DNAemia nor antibody responses to lytic/latent EBV antigens influence the longevity of both strain-specific and strain-transcending DBPII immune responses. Further studies should investigate the role of acute P. vivax infection in the activation of EBV replication cycle.
Heterogeneity analysis provides evidence for a genetically homogeneous subtype of bipolar-disorder
Authors: McGrouther Caroline C. , Rangan Aaditya V. , Di Florio Arianna , Elman Jeremy A. , Schork Nicholas J. , Kelsoe John ,   Bipolar Disorder Working Group of the Psychiatric Genomics Consortium
Published: January 2025

Background
Bipolar Disorder (BD) is a complex disease. It is heterogeneous, both at the phenotypic and genetic level, although the extent and impact of this heterogeneity is not fully understood. One way to assess this heterogeneity is to look for patterns in the subphenotype data. Because of the variability in how phenotypic data was collected by the various BD studies over the years, homogenizing this subphenotypic data is a challenging task, and so is replication. An alternative methodology, taken here, is to set aside the intricacies of subphenotype and allow the genetic data itself to determine which subjects define a homogeneous genetic subgroup (termed ‘bicluster’ below).

Results
In this paper, we leverage recent advances in heterogeneity analysis to look for genetically-driven subgroups (i.e., biclusters) within the broad phenotype of Bipolar Disorder. We first apply this covariate-corrected biclustering algorithm to a cohort of 2524 BD cases and 4106 controls from the Bipolar Disease Research Network (BDRN) within the Psychiatric Genomics Consortium (PGC). We find evidence of genetic heterogeneity delineating a statistically significant bicluster comprising a subset of BD cases which exhibits a disease-specific pattern of differential-expression across a subset of SNPs. This disease-specific genetic pattern (i.e., ‘genetic subgroup’) replicates across the remaining data-sets collected by the PGC containing 5781/8289, 3581/7591, and 6825/9752 cases/controls, respectively. This genetic subgroup (discovered without using any BD subtype information) was more prevalent in Bipolar type-I than in Bipolar type-II.

Conclusions
Our methodology has successfully identified a replicable homogeneous genetic subgroup of bipolar disorder. This subgroup may represent a collection of correlated genetic risk-factors for BDI. By investigating the subgroup’s bicluster-informed polygenic-risk-scoring (PRS), we find that the disease-specific pattern highlighted by the bicluster can be leveraged to eliminate noise from our GWAS analyses and improve risk prediction. This improvement is particularly notable when using only a relatively small subset of the available SNPs, implying improved SNP replication. Though our primary focus is only the analysis of disease-related signal, we also identify replicable control-related heterogeneity.
Nitroxyl protects H9C2 cells from H/R-induced damage and inhibits autophagy via PI3K/Akt/mTOR pathway
Authors: Li Li , Wang Zhixin , Lyu Yaxuan , Guo Yanqing 
Published: January 2025

Background
Myocardial ischemia-reperfusion injury (MIRI) is an important complication in the treatment of heart failure, and its treatment has not made satisfactory progress. Nitroxyl (HNO) showed protective effects on the heart failure, however, the effect and underlying mechanism of HNO on MIRI remain largely unclear.

Methods
MIRI model in this study was established to induce H9C2 cell injury through hypoxia/reoxygenation (H/R) in vitro. The cell viability was assessed by cell counting kit-8 assay. The effect of HNO on the apoptosis was detected by flow cytometry. DCFH-DA fluorescent probe method was applied to detect the level of intracellular reactive oxygen species (ROS). The morphology of mitochondria and autophagosomes were observed by transmission electron microscopy. Apoptosis, autophagy and PI3K/Akt/mTOR pathway-related proteins were detected by western blot.

Results
The viability of H9C2 cells was significantly increased in the HNO group. HNO inhibited apoptosis and regulated expressions of key apoptotic protein, including Bax and Bcl-2. HNO reduced ROS levels and alleviated H/R-induced mitochondrial damage. HNO also inhibited autophagy and regulated expressions of key autophagy-related molecules, including LC3II, p62 and Beclin1. Further experiments demonstrated that the effects of HNO were mediated through upregulation of PI3K/Akt/mTOR pathway. Rapamycin reversed the inhibition of HNO on H/R-induced autophagy in H9C2 cells, which abrogated the protective effect of HNO.

Conclusion
This study provided the first evidence that HNO protected H/R-induced cardiomyocytes through inhibiting autophagy via the activation PI3K/Akt/mTOR pathway.
Low levels of Plasmodium falciparum parasitaemia among patients receiving antiretroviral therapy at the treatment centre of the Regional Hospital Bamenda, Northwest Cameroon
Authors: Ebai Calvin Bisong , Yamssi Cedric , Ngoufo Flore Nguemaïm , Tchoffo Nicaise Ngouemeta , Nlinwe Omarine Nfor , Kimbi Helen Kuokuo 
Published: January 2025

Background
Malaria and HIV are leading causes of death in Africa, including Cameroon. Antiretroviral therapy (ART) is expected to boost immunity and reduce vulnerability to opportunistic infections. Reports on comorbidities including malaria are common in Cameroon.

Objectives
To determine the prevalence of malaria parasitaemia, clinical manifestations, treatment related factors and prevention methods associated with malaria parasitaemia as well as parasite density among HIV patients on ART.

Methods
It was a cross-sectional study among HIV patients on ART at the Regional Hospital Bamenda. A pre-tested questionnaire was used to collect data on participants’ socio-demographic characteristics, clinical manifestations, history of HIV treatment and malaria prevention methods. Microscopy was used for malaria parasite diagnosis and to determine white blood cell (WBC) count. Data was analysed using SPSS version 20.

Results
The study included 181 participants. The overall prevalence of malaria parasitaemia was 9.4%. Although there were no significant statistical differences, the following trends were observed in the results: 55-71 year old age group (14%) was most infected with malaria parasite with the highest prevalence among those on the 8A first-line ART (10.3%, 14/122); (χ2=1.03, p=0.96), only participants in the 8A and 12A ART protocols were infected; parasite prevalence was higher in patients with detectable viral load (14.3%, 3/21), (OR=.0.57 (CI: 0.15-2.2), p=0.41), while GMPD varied from 45.7-53.3-±23.1/μl of blood.

Conclusion
Low malaria parasite prevalence and density were detected amongst HIV patients receiving ART. A systematic malaria test could be helpful to avert morbidity and improve the general health of patients on ART.
Accuracy of latest large language models in answering multiple choice questions in dentistry: A comparative study
Authors: Nguyen Huy Cong , Dang Hai Phong , Nguyen Thuy Linh , Hoang Viet , Nguyen Viet Anh 
Published: January 2025

Objectives
This study aims to evaluate the performance of the latest large language models (LLMs) in answering dental multiple choice questions (MCQs), including both text-based and image-based questions.

Material and methods
A total of 1490 MCQs from two board review books for the United States National Board Dental Examination were selected. This study evaluated six of the latest LLMs as of August 2024, including ChatGPT 4.0 omni (OpenAI), Gemini Advanced 1.5 Pro (Google), Copilot Pro with GPT-4 Turbo (Microsoft), Claude 3.5 Sonnet (Anthropic), Mistral Large 2 (Mistral AI), and Llama 3.1 405b (Meta). χ2 tests were performed to determine whether there were significant differences in the percentages of correct answers among LLMs for both the total sample and each discipline (p < 0.05).

Results
Significant differences were observed in the percentage of accurate answers among the six LLMs across text-based questions, image-based questions, and the total sample (p<0.001). For the total sample, Copilot (85.5%), Claude (84.0%), and ChatGPT (83.8%) demonstrated the highest accuracy, followed by Mistral (78.3%) and Gemini (77.1%), with Llama (72.4%) exhibiting the lowest.

Conclusions
Newer versions of LLMs demonstrate superior performance in answering dental MCQs compared to earlier versions. Copilot, Claude, and ChatGPT achieved high accuracy on text-based questions and low accuracy on image-based questions. LLMs capable of handling image-based questions demonstrated superior performance compared to LLMs limited to text-based questions.

Clinical relevance
Dental clinicians and students should prioritize the most up-to-date LLMs when supporting their learning, clinical practice, and research.