bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endothelial inflammation in patients with Rheumatoid Arthritis treated with Tofacitinib
Authors: Vega María Celina De la , Riopedre Augusto Martín , Peón Claudia , Rodriguez Gonzalo , Benavidez Federico , Gomez Ramiro Adrián , Gamba María Julieta , Alfaro María Agustina , Eleta Martin , Benitez Cristian Alejandro 
Published: November 2023

Abstract

Introduction:
Cardiovascular involvement is frequent in patients with Rheumatoid Arthritis (RA). The use of tofacitinib has been linked with an increment in cardiovascular events in some populations of RA patients. 18F-Fluorodeoxyglucose Positron Emission Tomography (PET-FDG/TC) has emerged as a sensitive and specific test for the evaluation of vascular wall inflammation. The aim of this study is to evaluate the endothelial vascular inflammation using PET-FDG/TC in patients with active RA initiating tofacitinib, at baseline and after 12 weeks of treatment.
Methods
Observational, prospective, multicentric study. Consecutive patients with RA with moderate/high activity, bDMARD naïve, that were to start tofacitinib were included. Clinical data, disease activity and analytics were assessed. PET-FDG/TC was performed at baseline (week 0) and at week 12 of tofacitinib treatment. Endothelial inflammation was assessed using SUVmax and TBRmax. Carotid arteries doppler ultrasonography was performed at baseline and week 12 and intima-media thickness was measured.
Results
30 patients were included. 70% female, median age 57.5 (IQR 42–65) years old, median RA duration 5 (IQR 2–12) years, Median DAS28ESR 5.24 (IQR 4.6–6.1) median CDAI 27.5 (IQR 20–34). At week 12 of tofacitinib treatment, patients showed a significant decrease in disease activity by DAS28ESR (5.21 vs 3.04, p < 0.0001) and CDAI (26.6 vs 8.80, p < 0.0001) but 18F-FDG uptake in the five evaluated areas showed no significant difference between baseline and week 12 with all explored vascular showing a SUVmax over the prestipulated threshold defining inflammation at baseline.
Conclusion
In our study, we found no change in vascular inflammation at week 12 of tofacitinib treatment, despite improvement in disease activity.
Protocol for a Systematic Review and Meta-analysis of the Prevalence of Mental Illness Among Nursing Home Residents
Authors: Holt Jared , Bhar Sunil , Schofield Penelope , Koder Deborah , Owen Patrick , Seitz Dallas , Bhowmik Jahar 
Published: November 2023

Abstract
Background: There is a high prevalence of mental illness in nursing home residents compared to older adults living in the community. This was highlighted in the most recent comprehensive systematic review on the topic, published in 2010. An updated systematic review and meta-analysis is needed to provide a contemporary account of mental illness among nursing home residents, especially in the context of a rapidly aging population and increased numbers of older adults requiring residential care.
Methods: This protocol was prepared in line with the PRISMA-P 2015 Statement. Systematic searches will be undertaken in August 2023 across six electronic databases: PubMed, Embase, Web of Science, PsycNET, CINAHL, and Abstracts in Social Gerontology. Database searches will be supplemented by forward and backward citation searches of previously published reviews. Titles and abstracts of records will be screened using a semi‑automated process, facilitated by an online review management platform. The full text of selected records will subsequently be assessed to confirm inclusion criteria are met. Study selection will be recorded in a PRISMA flowchart. A pilot-tested form will be used to extract data from included studies. The JBI Critical Appraisal Checklist for Studies Reporting Prevalence Data will be used to undertake quality assessments of included studies. A summary of findings table will be prepared to present key details from each included study, supported by a narrative synthesis. Random-effects meta-analyses will be performed to compute pooled prevalence estimates for mental illnesses represented in the identified studies. Heterogeneity will be assessed using I2 statistics. A Funnel plot and Egger’s test will be used to assess publication bias. The GRADE approach will be used to assess the quality of the body of evidence identified.
Discussion: The study will provide a comprehensive and contemporary account of the prevalence of mental illness among nursing home residents. Meta-analyses will provide robust prevalence estimates across a range of presentations. Key insights will be highlighted, including potential sources of heterogeneity. Implications for residents, researchers, care providers, and policy makers will be noted.
Systematic review registration: PROSPERO: CRD42023456226
Erianin impedes the proliferation and metastatic migration through suppression of STAT-3 phosphorylation in human esophageal cancer cells
Authors: Hu Anxi , Kunkun Li 
Published: November 2023

Abstract
In this study, we have investigated erianin, a natural phenolic drug that impedes proliferation and metastatic migration through suppression of STAT-3 phosphorylation in human esophageal cancer cells. Eca-109 cells were treated with different concentrations of erianin (4, 8, 12 µM) for 24 hours, and then cell proliferation, apoptosis, and metastatic markers were evaluated. Erianin-induced cytotoxicity and cell proliferation were examined using MTT and crystal violet staining techniques. The measurement of reactive oxygen species (ROS) and the study of apoptotic changes were conducted through flow cytometry. Furthermore, protein expression analyses via western blotting included an evaluation of JAK-STAT3, cell survival, cell cycle, proliferation, and apoptosis-related proteins. Moreover, erianin treatment-associated MMP expressions were studied by RT-PCR. In this study, erianin treatment induces substantial cytotoxicity and ROS production based on the concentrations in Eca-109 cells. Moreover, erianin inhibits the MAPK phosphorylation, proliferation, and metastatic protein in Eca-109 cells. STAT-3 is a crucial transcriptional factor that regulates numerous downstream proteins, such as proliferation, anti-apoptosis, and metastatic proteins. In this study, erianin treatment inhibited the protein expression of IL-6, IL-10, JAK-1, and p-STAT-3 expressions leading to induce apoptosis in Eca-109 cells. Moreover, erianin inhibited the expression of proliferation, metastatic, and anti-apoptotic markers in Eca-109 cells. Hence, erianin suppressed the JAK/STAT-3 signaling pathway and demonstrates potential as a chemotherapeutic agent for the treatment of esophageal cancer.
A new prognostic signature of 11 E3-related genes for colon cancer related to the immune microenvironment and somatic mutation
Authors: Jiang Wanju , Dong Jiaxing , Xu Qitong , Cui Ran , Huang Zhiye , Guo Taohua , Zhang Kehui , Jiang Xiaohua , Du Tao 
Published: November 2023

Abstract
Background
Colon Cancer (COAD) is a common tumor in the gastrointestinal tract with a poor prognosis. It has been reported that ubiquitin-dependent modification systems influence tumor genesis and progression in various malignancies.
Methods
We collected the RNA expression data of the E3RGs from the TCGA-COAD program, used the “limma” R package to get differentially expressed E3RGs between COAD and healthy patients. Then we constructed the prognostic signature and calculated the risk score with univariate COX regression analysis and the LASSO analysis. We used a nomogram model to examine the predictive ability of the predictive model to predict OS rates at 1, 3, and 5 years. Next, we explored the significance of the predictive model under the stratified analysis. At last, we used bioinformatics and statistical methods to find some potential mechanisms in COAD cancer.
Results
We screened 137 E3-related genes (E3RGs), including 89 upregulated and 48 down-regulated E3RGs. Eleven genes (CORO2B, KCTD9, RNF32, BACH2, RBCK1, DPH7, WDR78, UCHL1, TRIM58, WDR72, and ZBTB18) were identified for the construction of prognostic signatures using univariate and multivariate Cox regression analysis, and lasso regression analysis. Kaplan-Meier curve analysis with log-rank testing showed a worse prognosis for patients with high risk based on the constructed E3RGs-based classifiers both in the train and test sets (P = 1.037e-05, P = 5.704e-03), and the proportion under ROC curves (AUC) was significant both in training and test groups (5-year AUC, 0.728 versus 0.892). Based on a stratified analysis, this 11-ERGs signature was a novel and attractive prognostic model independent of several clinicopathological parameters (age, sex, stage, TNM) in COAD. The enrichment and TME analysis of the signature confirmed that this signature might provide insight into the molecular mechanisms in COAD cancer.
Conclusions
We developed a novel independent risk model consisting of 11 ERGs and verified the effectiveness of this model in predicting the prognosis of COAD patients, providing important insights into survival prediction in COAD and several promising targets for COAD therapy.
Dwindling Groundwater Reserves Will Threaten Mountain Streamflow in a Warmer World
Authors: Carroll Rosemary , Niswonger Richard , Ulrich Craig , Varadharajan Charuleka , Siirila-Woodburn Erica , Williams Kenneth 
Published: November 2023

Abstract

Groundwater storage and release in mountain systems is often ignored compounding uncertainty in how these systems may respond to climate change. To assess the importance of groundwater to mountain streamflow, we combine an extensive data network with a high-resolution, integrated hydrologic model stretching into the deep subsurface for a Colorado River headwater basin. In a warmer climate, increased forest water use reduces recharge, thereby destabilizing groundwater storage such that it cannot recover to historical levels even during simulated wet periods. Groundwater depletion significantly reduces average annual streamflow and forces the basin toward non-perennial conditions during dry water years. Groundwater declines are amplified in high-relief, conifer-dominated catchments. Upscaling model results across the region indicates concentrated risk of groundwater decline in the Colorado Headwater and Gunnison Basins. Research highlights the tight coupling of topography, vegetation and groundwater dynamics and that excluding groundwater response to warming will underestimate future reductions in mountain streamflow.
A Time-domain Runge-Kutta Dual Reciprocity Boundary Element Method for Scalar Wave Propagation
Authors: Zhou Fenglin , Zhao jiaao , Yu Qin , Li Wenyuan , Yu Jianghong 
Published: November 2023

Abstract
In this paper, the dual reciprocity boundary element method combined with the Runge- Kutta method is applied to solve the acoustic radiation problem. In the dual reciprocity scheme, the radial basis function is introduced to transform the equivalent integral equation into an equivalent integral equation of a pure boundary form. After the boundary elements discretization, the second-ordered ordinary differential equations (ODEs) are obtained. Furthermore, these ODEs are transformed into first-order ODEs (Euler equations) by introducing a median variable. The Runge-Kutta method of the fourth order is applied to solve these Euler equations. Two numerical examples of acoustic problems on different structures are presented to illustrate the validity and the accuracy of the proposed method.
Bones embedded in muscles structure-inspired flexible wide-range multidimensional force sensors
Authors: Hou Xiaojuan , Zhang Jie , Qian Shuo , Huo Jiabing , Yuan Mengjiao , Duan Zhigang , Song Xiaoguang , Wu Hui , Shi Shuzheng , geng wenping , Mu Jiliang , He Jian , Chou Xiujian 
Published: November 2023

Abstract
Flexible sensors have been widely studied in motion monitoring, human-machine interaction (HMI), personalized medicine, and soft intelligent robots. However, their practical application is limited by the low output performance, the narrow measuring range, and the unidirectional force detection. Here, to achieve flexibility and high performance simultaneously, we developed a flexible wide-range multidimensional force sensor (FWMFS) like bones embedded in muscles structure. The adjustable magnetic field renders FWMFS with multidimensional perception for detecting forces in different directions. The multilayer stacked coils significantly improve the output from µV to mV level while ensuring FWMFS miniaturization. The optimized FWMFS exhibits a high voltage sensitivity of 0.227mV/N (0.5-8.4N) and 0.047mV/N (8.4-60N) responding to normal forces in the range 0 to 60N and can detect the lateral force with a range of 0.2-1.1N, a voltage sensitivity of 1.039mV/N (0.2-0.5N) and 0.194mV/N (0.5-1.1N). In terms of normal force measurement, FWMFS exhibits a wide range by monitoring finger pressure and sliding trajectories in response to finger taps, as well as measuring plantar pressure for assessing human movement. The plantar pressure signals of five human movement collected by FWMS are analyzed using the k-Nearest Neighbors classification algorithm with a recognition accuracy of 92%. Additionally, an artificial intelligence biometric authentication system is being developed that classifies and recognizes user passwords. Based on the lateral force measurement ability of FWMFS, the direction of ball movement can be distinguished, and communication systems like Morse code can be expand. This research has significant potential in intelligent sensing and personalized spatial recognition.
Rapid and efficient stem segment regeneration propagation system for Euonymus bungeanus
Authors: Cui Kai-Ge , Wang Hao-Lin , Quan Wen-Yue , Ye Zhuo-Zhuo , Zhao Cheng-Ri 
Published: November 2023

Abstract

Euonymus bungeanus Maxim. is a tree species with high ornamental, industrial, and medicinal value. Establishing a method for rapid and efficient regeneration of E. bungeanus is essential to achieve industrial-scale production. The aim of this study was to establish a rapid tissue propagation technique for E. bungeanus and provide a foundation for the industrial production of tissue-cultured seedlings. Using stem segments of E. bungeanus as explants, we investigated effects of explant collection time, sterilization method, various culture media, and ratios of plant growth regulators on the initiation, subculture, and rooting stages of the tissue culture process for E. bungeanus. The optimal explant collection time was mid-April; a combination of 75% ethanol for 20 s, followed by 0.1% HgCl2 for 7 min was suitable for disinfection, yielding a survival rate of 55.00% for the explants. Initiation culture using the woody plant medium (WPM) supplemented with 1.0 mg L-1 of 6-benzylaminopurine (6-BA) and 0.2 mg L-1 of α-naphthalene acetic acid (NAA) achieved an induction rate of 87.22% for explants. Propagation culture on ¼ WPM medium containing 2.0 mg L-1 of 6-BA and 0.1 mg L-1 of NAA resulted in a propagation coefficient of 4.98. Rooting culture on ½ Murashige and Skoog medium supplemented with 0.2 mg L-1 of indole-3-butyric acid achieved a rooting rate of 78.33%. However, the rooting rate was low, indicating the need for further optimization of rooting and acclimatization. This study is the first to develop a rapid and efficient regeneration system for E. bungeanus using stem segments.
Design of Acoustic Meta-surfaces for Underwater Stealth in Low Frequency Ranges
Authors: Ranjbar Mostafa , Bayer Muhammed Umar 
Published: November 2023

Abstract
In this study, it is aimed to protect underwater vehicles against active sonar systems by anechoic metamaterial coating. A mixture of matrix material with close acoustic impedance to water, resistivity to hydrostatic pressure, suitability for the marine environment, and high material loss factor are selected. At low frequencies, the inclusions in different shapes and sizes are added to the matrix material. Since solid inclusions will increase the density considerably, air cavities are preferred as inclusions. More attention is paid to low frequency absorption, especially below 1 kHz, because of advancing sonar technology. The acoustic performance of the designed models is compared in three frequency range: low (0–3 kHz), middle (3–6 kHz), and high (6–10 kHz). The designed models are constructed by considering hydrostatic pressure; hence, volume of air cavities is tried to decrease while absorption performance is aimed to increase. Therefore, a conical air cavity is optimized by chancing its dimensions and location. Also, novel approaches, gong shape air cavity, and sandglass air cavities are introduced. The results show that, not only cavity shape, but also its location and dimensions are highly influential on absorption performance. High volume air cavities increase the absorption performance at the low frequency range, but they are not effective at high frequencies. The gong shape, and sandglass air cavities shows broadband absorption, also, gong shape air cavity volume is less than literature models. Thus, its usability increases at deep waters. The results of this study provide novel underwater meta surfaces for various applications.
Seasonal impact on embryogenic callus induction under subtropical conditions in banana (Musa spp. cv. Grand Naine [AAA])
Authors: Marimuthu Kumaravel , Patel Prashanti , Weil Amir , Kozin Laura , Barak Shira Raikin , Gortikov Maggie , Peres Moti , Or Gal , Bakhshian Ortal , Elingold Idan , Tsarfaty Avi , Raz Amir , Galpaz Navot 
Published: November 2023

Abstract
Gene editing tools for banana crop improvement necessitates efficient embryogenic cell suspensions, which are derived through embryogenic calli (EC). Although many factors are known to influence EC formation, the roles of seasonal effects and environmental factors in EC induction of banana remain unclear. We therefore examined the formation of EC from immature male flower buds (IMFB) collected in each month of the years 2020 and 2022. Among 12 batches examined, IMFB initiated between January and April produced the highest percentage of EC in both years. In 2020, the percent of EC ranges from 8.22 to 12.14, whereas in 2022 the EC percentage ranges from 2.50 to 4.47. IMFB initiated from May to August produced moderate response and those initiated between September and December gave the lowest percentage of EC in both the years. Plants that generated highest percentage of EC underwent transition from vegetative to reproductive phase in autumn and flowered in winter. During this period, environmental factors such as global radiation, relative humidity and temperature were relatively low compared to summer. In contrast, plants that underwent vegetative–reproductive transition under extreme summer conditions and flowered at the end of summer and autumn yielded lowest percentage of EC. Exposure to high temperature and radiation during this period might have altered inflorescence development. Our results indicate that seasonal alterations in environmental factors influence the rate of EC induction.
Esketamine pretreatment during cesarean section reduced the incidence of PPD: A randomized controlled trail
Authors: Xu Shixia , Yang Jiaojiao , Li Jing , Zhang Min , Sun Jie , Liu Qingren , Yang Jianjun 
Published: November 2023

Abstract
Background
Postpartum depression (PPD) is a common mental disease in postpartum women, which has received more and more attention in society. Ketamine has been confirmed for its rapid antidepressant effect in women with PPD. We speculate that esketamine, an enantiomer of ketamine, pretreatment during cesarean can also reduce the incidence of PPD.
Methods
All the parturients enrolled in the study were randomly assigned to two groups: the esktamine group (0.2 mg/kg esketamine) and the control group (a same volume of saline). All the drugs were pumped for 40 min started from the beginning of the surgery. The Amsterdam Anxiety and Information Scale (APAIS) scores before the surgery, the Edinburgh postnatal depression scale (EPDS) scores at 4 d and 42 d after surgery, the Pain Numerical Rating Scale (NRS) scores at 6 h, 12 h, 24 h and 48 h post-operation were evaluated, as well as the adverse reactions were recorded.
Results
A total of 319 parturients were analyzed in the study. The incidence rate of PPD (EPDS score > 9) in the esketamine group was decreased than the control group at 4 days after surgery (13.8% vs 23.1%, P = 0.0430 ) but not 42 days after surgery (P = 0.0987). Esketamine 0.2 mg/kg could reduce the NRS score at 6 h,12 h and 24 h after surgery, as well as the use of vasoactive drugs during surgery (P < 0.05). The incidences of maternal dizziness (17.0%), blurred vision (5%), illusion (3.8%) and drowsiness (3.8%) in the esketamine group were higher than those of control group (P < 0.05).
Conclusions
Intraoperative injection of esketamine (0.2 mg/kg) prevented the occurrence of depression (EPDS score > 9) at 4 days after delivery but not 42 days. Esketamine reduced the NRS scores at 6 h, 12 h and 24 h after surgery, but the occurrence of maternal side effects such as dizziness, blurred vision, drowsiness and hallucination were increased.
Trial registration:
Registered in the Chinese Clinical Trial Registry (ChiCTR2100053422) on 20/11/2021.
Autopolyploidization effect on morpholological, anatomical, reproductive variations and andrographolide content in Andrographis paniculata (Burm. f.) Nees.
Authors: PRIYA LEKSHMI , P Shibin Felix , PILLAI P R UNNIKRISHNA 
Published: November 2023

Abstract
Artificial polyploidy induction plays a major role in improving various characteristics of the plants. Polyploidization has found widespread application in the breeding of new varieties of medicinal plants due to its numerous advantages and superior performance when compared to their diploid counterparts. This study was undertaken to evaluate the impact of artificial polyploidy on various characteristics of the medicinal plant Andrographis paniculata (Burm. f.) Nees. Aqueous colchicine was applied to Kalmegh seedlings at three different concentrations: 0.05%, 0.1%, and 0.2% (w/v), with varying exposure durations of 6, 12, and 24 h. The duplication of DNA content in induced polyploid plants was confirmed by employing flow cytometry analysis. The most successful induction of polyploidy was accomplished by administering 0.1% (w/v) colchicine for duration of 12 h. The polyploid plants achieved were shorter but with thicker stems, larger leaves, increased leaf area and thickness, larger seeds and flower characteristics than their diploid intact plants. The length and width of stomata were increased in induced polyploid plants, whereas stomata density and index was decreased, in contrast with initial diploid plants. This work follows a facile immersion method and also includes the stomatal and seed detailing of polyploid A. paniculata using SEM. High Performance Liquid Chromatography (HPLC) analysis showed notable (200 fold) increases in andrographolide content of polyploid plants (1.6mg/100g) in contrast with those of diploid plants (0.008mg/100g). Polyploidization also holds significant potential as a breeding technique for future breeding programs involving A. paniculata.