bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of Therapeutic Potential of Biosynthesized Silver and Gold
Nanoparticles Using Extract of Wrightia tinctoria
Authors: Kadam Shivani V. , Magdum Chandrakant S. , Kane Sandip R. , Bhutkar Mangesh A. , Randive Dheeraj S. , Bhinge Somnath D. , Sonawane Kailas D. 
Published: October 2023

Background:
In Indian traditional medicine, the seeds and bark of Wrightia tinctoria are
utilized as remedies for antidiarrheal and antidysenteric purposes, as well as for other medicinal uses.

Aim:
The primary aim of the study was to explore the green synthesis of silver and gold
nanoparticles by employing an extract obtained from the Wrightia tinctoria plant and to explore their
potential medicinal properties.

Objective:
This study involved the characterization of the nanoparticles in terms of their properties
and quality, as well as an investigation of their potential anti-bacterial, anticancer, and antiinflammatory
properties.

Methods:
Various characterization techniques, including UV spectroscopy, XRD spectra, FTIR,
SEM, particle size and zeta potential analysis, were used in this study for the synthesized
nanoparticles. Our study investigated the impact of concentration, pH, and incubation time on
nanoparticle synthesis, providing a comprehensive description of the synthesis procedure for both
silver and gold nanoparticles.

Result:
Experimental findings confirmed that silver and gold nanoparticles derived from Wrightia
tinctoria exhibited irregular shape, with an average diameter ranging from approximately 0.08 to
0.34 μm and 0.09 to 0.30 μm, respectively. Appreciably, the biologically synthesized WTAgNPs and
WTAuNPs demonstrated promising antibacterial, anticancer, and anti-inflammatory properties
without any signs of toxicity. The enhanced biological activity of WTAgNPs and WTAuNPs can be
attributed to their distinctive properties at the nanoscale, as both exhibit lower polydispersity and
average particle size, contributing to increased reactivity and interactions with biological systems.

Conclusion:
The nanoparticles synthesized through the biogenic approach using Wrightia tinctoria
extract have immense potential for a wide range of pharmaceutical applications.
MicroRNAs and Diet-induced Weight Loss: What’s the Link?
Authors: Cannataro Roberto , Abrego-Guandique Diana M. , Caroleo Maria C. , Bonilla Diego A. , Cione Erika 
Published: October 2023

Abstract:
It is now well established that lifestyle, particularly eating habits, modulates the syn-thesis and action of microRNAs (miRNAs). In particular, several nutritional schemes have proven effective in improving body composition, but molecular mechanisms still need to be fully under-stood. Within the complex physiological network of food intake regulation, it is essential to un-derstand the changes in endocrine activity after the reduction of adipose tissue during a weight loss program. This could be the key to identifying the optimal endocrine profile in high respond-ers, the assessment of musculoskeletal status, and long-term management. In this review, we summarize the state of the art regarding miRNAs as a function of weight loss and as a mechanistic regulator of the effectiveness of the nutritional program.
Formulation of Lipid Nanoparticles based Nanogel of Sertaconazole
Nitrate and its Evaluation
Authors: Pawar Sarvesh S. , Gadekar Punam S. , Rane Bhushan R. , Jain Ashish S. 
Published: October 2023

Background:
Sertaconazole nitrate is a topical antifungal drug used to treat interdigital tinea pedis in patients with immunocompetent conditions. The class of imidazole includes the antifungal medication sertaconazole nitrate. It is available in topical formulations for treating skin infections, including athlete's foot. Solid lipid nanoparticles (SLN) are at the cutting edge of nanotechnology, with several potential uses in drug delivery and research. Because of their unique size-dependent features, lipid nanoparticles hold the promise of novel therapies.

Objective:
Drug incorporation into nanocarriers creates a new drug delivery prototype that could be employed for drug targeting. The research aims to study the formulation and evaluation of Sertaconazole nitrate solid lipid nanoparticles. The goal behind formulating SLN gel is to provide and maintain therapeutic concentrations of the drug at the target biological site to maximise therapeutic efficacy and minimise side effects.

Methods:
Sertaconazole Nitrate Solid Lipid Nanoparticles are prepared by using High Pressure Homogenizer to get nanogel formulation as the final formulation and In-vitro drug release using a diffusion apparatus. The prepared SLNs were evaluated in their FTIR studies to determine compatibility between the drug and the excipients; zeta potential indicates the solid lipid nanoparticle was stable, and polydispersity index was used to determine particle size.

Result:
The results demonstrate that optimised SLN-based Sertaconazole nitrate gel exhibited the best physicochemical properties, including FTIR studies of the drug, excipients, and optimised formulation demonstrate that all are compatible with each other, particle size is less than 200 nm, zeta potential ranging from 12 to -20 mV, and highest entrapment efficiency is 71.48%. Optimised solid lipid nanoparticles showed good in vitro release and antimicrobial results. The main application of SLN large scale-up is possible, and the drug can be effective with less dose incor-poration.

Conclusion:
In this research work, the proposed plan of work SLN of Sertaconazole Nitrate was formulated successfully. The preliminary identification tests were performed, such as melting point determination, estimation of λmax by UV-visible spectrophotometry and plot of its calibration curve in solvent and buffer system, and FT-IR investigation to confirm the purity and confirmation of medication. High physical stability and drug loading are advantageous to SLN.
Ultrastructural Blood Cell Changes in Patients with COVID-19
Authors: Kyyak Yulian , Barnett Olga , Halkevych Marta , Labinska Olha , Kysil Orysia 
Published: October 2023

Introduction::
Nowadays, the coronavirus disease COVID-19 is a global problem for the
population of the whole world which has acquired the character of a pandemic. Under physiological
conditions, in a healthy person, erythrocytes make up 96 % of all blood cells, leukocytes 3 %, and
thrombocytes about 1 %. In healthy individuals, erythrocytes are mostly shaped like a biconcave disc
and do not contain a nucleus. The diameter of the erythrocyte is 8 microns, but the peculiarities of
the cell structure and the membrane structure ensure their great ability to deform and pass through
capillaries with a narrow lumen of 2-3 microns. Therefore, the study of the morpho-functional state
of blood cells, namely erythrocytes, in this category of patients is relevant and deserves further research.

The Aim:
To figure out the effect of the coronavirus disease COVID-19 on the ultrastructural blood
cell changes, in particular erythrocytes, in patients with ischemic heart disease (IHD) and diabetes
mellitus type 2.

Materials and Methods:
Twelve patients with COVID-19 who had an acute myocardial infarction
were examined. The comparison group consisted of 10 people with acute myocardial infarction
without symptoms of COVID-19. The average age of the patients was 62 ± 5,6 years. The functional
state and ultrastructure of blood cells were studied using electron microscopy.

Results::
In the presence of COVID-19, we detected both calcification and destruction of erythrocytes
and platelets. Reticulocytes were detected much more often in these individuals than in the comparison
group. In patients with acute myocardial infarction in the presence of type 2 diabetes and
COVID-19, a significant number of markedly deformed, hemolyzed erythrocytes or with signs of
acanthosis, which stuck together and with other destructively changed blood cells, were found. We
also detected «neutrophils extracellular traps» (NETs).

Conclusions::
Morphological changes of blood cells in COVID-19 varied according to the disease
course and severity especially in the background of a weakened immune system in older and elderly
people, in the presence of diabetes, excessive body weight, cardiovascular diseases and occupational
hazards. Under the influence of COVID-19, blood cells are destroyed by apoptosis and necrosis.
Therefore, hypoxia and ischemia of vital organs of the human body occur.
The Association between Statins Intake and Risk of Post Stroke Pneumonia:
A Systematic Review and Meta-Analysis
Authors: Darvishi Mohammad , Amiri Mehdi Mohammadian , Heidari-Soureshjani Saeid , Sherwin Catherine M.T. , Mardani-Nafchi Hossein 
Published: October 2023

Aim:
This research aimed to examine the relationship between the intake of statins and
the risk of post-stroke pneumonia in a systematic review and meta-analysis study.

Methods:
An extensive search of published articles on March 21st, 2023, was done in several databases,
like Web of Science (ISI), PubMed, Cochrane Library, Embase, Scopus, and Google Scholar.
The Newcastle Ottawa Scale (NOS) checklist was employed to evaluate the quality of observational
studies. Statistical tests (Chi-square test and I2) and graphical techniques (Forest plot) were used to
determine whether heterogeneity existed in the meta-analysis studies. Funnel plots and Begg and
Egger's tests were used to assess the publication bias.

Results:
Seven studies (5 cohort and 2 case-control studies) were retrieved to examine the association
between statins and post-stroke pneumonia. The sample size of the studies compiled in the meta-
analysis was obtained to be 68,966 participants. Meta-analysis demonstrated that the overall odds
of post-stroke pneumonia in the statin group was equal to 0.87 (95% CI: 0.67 – 1.13; p-value
0.458). Subgroup analysis indicated that the odds of post-stroke pneumonia in the statin group was
equal to 0.93 (95% CI: 0.73-1.18; p-value = 0.558) in the cohort studies, and equal to 0.92 (95% CI:
0.37-2.26; p-value = 0.857) in the case-control studies. The examination of the association between
the intake of statins and post-stroke pneumonia showed no evidence of publication bias (Begg's test,
p-value = 0.368; Eggers test, p-value = 0.282).

Conclusion:
In this study, no relationship has been observed between receiving statins and the risk
of post-stroke pneumonia.
Immune-Boosting Effect of the COVID-19 Vaccine: Real-World Bidirectional Cohort Study
Authors: Liu Ming , Zhao Tianshuo , Mu Qiuyue , Zhang Ruizhi , Liu Chunting , Xu Fei , Liang Luxiang , Zhao Linglu , Zhao Suye , Cai Xianming , Wang Mingting , Huang Ninghua , Feng Tian , Lei Shiguang , Yang Guanghong , Cui Fuqiang 
Published: October 2023

Background
As the SARS-CoV-2 attenuates and antibodies from the COVID-19 vaccine decline, long-term attention should be paid to the durability of primary booster administration and the preventive effect of the second or multiple booster doses of the COVID-19 vaccine.

Objective
This study aimed to explore the durability of primary booster administration and the preventive effect of second or multiple booster doses of the COVID-19 vaccine.

Methods
We established a bidirectional cohort in Guizhou Province, China. Eligible participants who had received the primary booster dose were enrolled for blood sample collection and administration of the second booster dose. A retrospective cohort for the time of administration was constructed to evaluate antibody attenuation 6-12 months after the primary booster dose, while a prospective cohort on the vaccine effect of the second booster dose was constructed for 4 months after the second administration.

Results
Between September 21, 2022, and January 30, 2023, a total of 327 participants were included in the final statistical analysis plan. The retrospective cohort revealed that approximately 6-12 months after receiving the primary booster, immunoglobulin G (IgG) slowly declined with time, while immunoglobulin A (IgA) remained almost constant. The prospective cohort showed that 28 days after receiving the second booster, the antibody levels were significantly improved. Higher levels of IgG and IgA were associated with better protection against COVID-19 infection for vaccine recipients. Regarding the protection of antibody levels against post–COVID-19 symptoms, the increase of the IgG had a protective effect on brain fog and sleep quality, while IgA had a protective effect on shortness of breath, brain fog, impaired coordination, and physical pain.

Conclusions
The IgG and IgA produced by the second booster dose of COVID-19 vaccines can protect against SARS-CoV-2 infection and may alleviate some post–COVID-19 symptoms. Further data and studies on secondary booster administration are required to confirm these conclusions.
Efficacy and Safety of Makabuhay (Tinospora rumphii) 25% Cream Versus Hydrocortisone 1% Cream in the Management of Mosquito Bite Reactions: Randomized Double-Blind Controlled Trial
Authors: Gatmaitan Julius Garcia , Gatmaitan-Dumlao Jolene Kristine Garcia , Dayrit Johannes , Gabriel Ma Teresita 
Published: October 2023

Background
Most insect bite reactions resolve spontaneously, but the inflammation and pruritus induced have been shown to decrease the quality of life. Previous studies have shown the potential anti-inflammatory properties of Tinospora rumphii.

Objective
The aim of the study is to assess the efficacy and safety of T rumphii 25% cream versus hydrocortisone 1% cream in the management of local cutaneous reactions caused by mosquito bites.

Methods
This study was a parallel-group, double-blind, randomized, placebo-controlled trial with a 1-week duration in a span of 3 months (June 2019 to August 2019). Participants were exposed to sterile noninfectious mosquitoes (Aedes aegypti) for 5-10 minutes to elicit cutaneous lesions. Tinospora 25% cream or hydrocortisone 1% cream was applied twice daily throughout the 7-day study period. Of the 70 participants screened for this study, which was approved by an institutional review board (IRB 2019-07) at the Dermatology Outpatient Department of the Research Institute for Tropical Medicine, Alabang, Muntinlupa, Philippines, 58 participants in total met the inclusion criteria and were randomized to treatment (Tinospora: n=29) and active control (hydrocortisone: n=29) groups.

Results
In total, 58 participants were randomized to receive Tinospora cream (n=29) or hydrocortisone cream (n=29). All participants completed the follow-up. There was a significant decrease in lesion size in both groups from the first 15 minutes to day 7 (P<.001). Comparing the lesion size in both groups, there was a statistically significant decrease in lesion size in the first hour (P=.003) and after 24 hours (P=.03). On day 1, 10% (n=29) of participants in the hydrocortisone group and 7% (n=29) in the Tinospora group experienced complete resolution. On day 3, all participants experienced complete resolution. No adverse effects were documented.

Conclusions
Tinospora 25% cream is safe, effective, and comparable to hydrocortisone 1% cream as an anti-inflammatory agent for mosquito bite reactions based on the decrease in lesion size, the proportion of participants with complete resolution of wheals, and improvement in pruritus intensity score using a visual analog scale. Long-term safety studies are recommended.

Trial Registration
Philippine Health Research Registry PHRR230716-005932; https://www.herdin.ph/index.php/registry?view=research&layout=details&cid=5932
Flexibilidad cognitiva y rendimiento académico en estudiantes de primer año de medicina
Authors: Jaimes Medrano Aurora Leonila , Fossion Ruben , Flores Lázaro Julio , Caraveo-Anduaga Jorge Javier 
Published: October 2023

Introducción: Diversas investigaciones han demostrado que la flexibilidad cognitiva predice el rendimiento académico, principalmente en escolares, pero solo unas pocas han demostrado la participación de la flexibilidad cognitiva en el rendimiento académico de estudiantes universitarios.
Objetivo: Analizar la asociación entre la flexibilidad cognitiva y el rendimiento académico de los estudiantes de primer año de medicina; además de algunos antecedentes socioeconómicos que influyen en el desarrollo y en el rendimiento académico.
Método: Se realizó un estudio descriptivo de corte transversal, en una muestra de 406 estudiantes de primer año de medicina, se evaluó la flexibilidad cognitiva (con el programa informático WisConPC) y los antecedentes socioeconómicos, como factores asociados al rendimiento académico de estudiantes de medicina mediante un análisis de regresión lineal múltiple.
Resultados: El estudio muestra que la flexibilidad cognitiva y el contexto sociocultural son factores asociados al rendimiento académico general, particularmente en materias biomédicas.
Conclusiones: Los hallazgos de este estudio tienen implicaciones para el proceso de enseñanza-aprendizaje de las ciencias biológicas y químicas, ya que los docentes pueden promover la flexibilidad cognitiva utilizando estrategias didácticas dirigidas al desarrollo y uso de operaciones mentales que implican la formulación de inferencias y la aplicación de ecuaciones proporcionales, razonamiento lógico y probabilístico, contribuyendo así a la construcción y transformación del conocimiento de los estudiantes.
Kualitas Starter Sourdough dengan Penambahan Sari Salak
Authors: Nataresa I Wayan Bayu Banen , Kuntariati Utik 
Published: October 2023

Starter sourdough adalah salah satu olahan ragi alami yang dibuat dari produk tepung, dan air. Penelitian bertujuan guna memahami kualitas starter sourdough dengan penambahan sari salak. Metode penelitian yang dipergunakan dalam penelitian yaitu metode penelitian kuantitatif, kualitatif dengan jenis penelitian eksperimental dan teknik pengumpulan data yang dilakukan secara observasi juga kuesioner. Dalam menentukan kualitas dari starter sourdough dengan penambahan sari salak dinilai dengan menggunakan teknik uji organoleptik yang diujikan kepada 30 responden yang mendapatkan hasil penelitian bahwa starter sourdough dengan penambahan 100% sari salak (P1) memiliki kualitas keunggulan dibagian tekstur yang sangat lembut. Dalam aspek rasa dan aroma, sourdough dengan penambahan sari salak atau tanpa penambahan sari salak memiliki keunggulannya masing masing dari setiap responden.

Starter sourdough is one of the natural yeast preparations made from flour and water products. The purpose of this study was to determine the quality of starter sourdough with the addition of snakefruit juice. The research method applied in this study is a qualitative, quantitative research method with the type of experimental research and data collection techniques carried out by observation as well as questionnaires. In determining the quality of starter sourdough with the addition of snakefruit juice, it was assessed using an organoleptic test technique that was tested on 30 respondents who got the results that sourdough starter with the addition of 100% salak juice (P1) had superior quality in the very soft texture. In terms of taste and aroma, sourdough with the addition of snakefruit juice or without the addition of salak juice has its own advantages from each respondent.
Pengaruh Kepemimpinan dan Motivasi Kerja Terhadap Kinerja Karyawan
Authors: Kosala I Ketut Hery , Pantiyasa I Wayan , Wijaya Nyoman Surya 
Published: October 2023

Penelitian ini bertujuan untuk mengetahui pengaruh kepemimpinan dan motivasi kerja terhadap kinerja karyawan. Penelitian ini dilakukan pada The Pond Restaurant Discovery Kartika Plaza Hotel. Populasi yang digunakan dalam penelitian ini adalah 40 orang karyawan dengan seluruh populasi digunakan menjadi sampel. Teknik analisis data yang digunakan adalah analisis regresi linier berganda. Hasil penelitian menunjukkan bahwa kepemimpinan dan motivasi kerja secara bersama – sama berpengaruh terhadap kinerja karyawan adalah, hal ini diperoleh dari nilai signifikansi F sebesar 0,000. Kepemimpinan terhadap kinerja karyawan adalah pengaruh yang positif, hal ini diperoleh dari nilai signifikansi sebesar 0,008. Pengaruh motivasi kerja terhadap kinerja karyawan adalah pengaruh yang positif, hal ini diperoleh dari nilai signifikansi sebesar 0,000. Berdasarkan hasil analisis data menunjukan bahwa besarnya pengaruh kepemimpinan dan motivasi kerja terhadap kinerja karyawan sebesar 84,3%, sementara sisanya sejumlah 15,7% dipengaruhi oleh faktor – faktor lain diluar penelitian ini.

This study aims to determine the influence of leadership and motivation work on employee performance. This research was conducted at The Pond Restaurant Discovery Kartika Plaza Hotel. The population used in this study was 40 employees with the entire population used as a sample. Data analysis technique used is multiple linear regression analysis. The research results show that leadership and work motivation jointly influence performance employees is, this is obtained from the significance value of F of 0.000. Leadership on employee performance is a positive influence, this is obtained from the value a significance of 0.008. The effect of work motivation on employee performance is positive influence, this is obtained from a significance value of 0.000. Based on the results of data analysis show that the magnitude of the influence of leadership and work motivation on employee performance by 84.3%, while the remaining 15.7% is affected by other factors outside of this study.
Pengaruh Pelatihan dan Disiplin Kerja Terhadap Kinerja Karyawan
Authors: Parta I Ketut Wira , Ismail Deden , Wijaya Nyoman Surya 
Published: October 2023

Penelitian ini bertujuan untuk mengetahui apakah Pelatihan (X1) dan Disiplin Kerja (X2) mempengaruhi Kinerja Karyawan (Y) baik secara simultan atau secara parsial di Nandini Jungle Resort & Spa, Payangan Gianyar. Tempat dilaksanakan penelitian ini adalah di Nandini Jungle Resort & Spa, Payangan Gianyar. Waktu penelitian dilaksanakan pada bulan November 2020 – Juli 2021. Populasi dari penelitian ini adalah 44 yang sekaligus dijadikan sampel sehingga menggunakan metode sampel jenuh. Pengumpulan data menggunakan observasi, wawancara, dokumentasi, kuesioner. Jenis penelitian adalah penelitian korelasional. Kemudian dilakukan analisis secara kuantitatif, yang meliputi: uji validitas, uji reliabilitas, analisis regresi linier berganda dan uji asumsi klasik dengan menggunakan SPSS release 25. Uji hipotesis menggunakan uji F dan uji t. Hasil penelitian menunjukkan pelatihan berpengaruh positif dan signifikan (p<0,05) terhadap kinerja karyawan. Disiplin kerja berpengaruh positif dan sangat signifikan (p<0,01) terhadap kinerja karyawan. Kemudian, Pelatihan dan Disiplin Kerja berpengaruh positif dan sangat signifikan (p<0,01) terhadap Kinerja Karyawan.

This study aims to determine whether training (X1) and work discipline (X2) affect Employee Performance (Y) either simultaneously or partially at Nandini Jungle Resort & Spa, Payangan Gianyar. The place where this research was carried out was at Nandini Jungle Resort & Spa, Payangan Gianyar. The time of the study was carried out in November 2020 - July 2021. The total population was 44 people which was also used as a sample so that it used the saturated sample method. Collecting data using observation, interview, documentation, questionnaire. This type of research is correlational research. Then a quantitative analysis was carried out, which included: validity test, reliability test, multiple linear regression analysis and classical assumption test using SPSS release 25. Hypothesis test using F test and t test. The results showed that training had a positive and significant effect (p<0.05) on employee performance. Work discipline has a positive and very significant effect (p<0.01) on employee performance. Then, Training and Work Discipline have a positive and very significant effect (p<0.01) on Employee Performance.