bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐cell analysis of MYD88L265P and MYD88WT Waldenström macroglobulinemia patients
Authors: Bagratuni Tina , Aktypi Foteini , Theologi Ourania , Sakkou Maria , Verrou Kleio Maria , Mavrianou‐Koutsoukou Nefeli , Patseas Dimitrios , Liacos Christine , Skourti Stamatia , Papadimou Alexandra , Taouxi Kostantina , Theodorakakou Foteini , Kollias Georgios , Sfikakis Petros , Terpos Evangelos , Dimopoulos Meletios A. , Kastritis Efstathios 
Published: February 2024

AbstractWaldenström macroglobulinemia (WM) is characterized by the expansion of clonal lymphoplasmacytic cells; the MYD88L265P somatic mutation is found in >90% of patients, but malignant B cells may still display intra‐clonal heterogeneity. To assess clonal heterogeneity in WM, we generated and performed single‐cell RNA sequencing of CD19+ sorted cells from five patients with MYD88L265P and two patients with MYD88WT genotype as well as two healthy donors. We identified distinct transcriptional patterns in the clonal subpopulations not only between the two genetically distinct WM subgroups but also among MYD88L265P patients, which affected the B cell composition in the different subgroups. Comparison of clonal and normal/polyclonal B cells within each patient sample enabled the identification of patient‐specific transcriptional changes. We identified gene signatures active in a subset of MYD88L265P patients, while other signatures were active in MYD88WT patients. Finally, gene expression analysis showed common transcriptional features between patients compared to the healthy control but also differentially expressed genes between MYD88L265P and MYD88WT patients involved in distinct pathways, including NFκΒ, BCL2, and BTK. Overall, our data highlight the intra‐tumor clonal heterogeneity in WM with potential prognostic and therapeutic implications.
Developing the future research agenda for the health and social care workforce in the United Kingdom: Findings from a national forum for policymakers and researchers
Authors: Lamont Tara , Chatfield Cat , Walshe Kieran 
Published: February 2024

AbstractThere is a gap between healthcare workforce research and decision‐making in policy and practice. This matters more than ever given the urgent staffing crisis. As a national research network, we held the first ever United Kingdom (UK) forum on healthcare workforce evidence in March 2023. This paper summarises outputs of the event including an emerging UK healthcare workforce agenda and actions to build research capacity and bridge the gap between academics and decisionmakers. The forum brought together over 80 clinical and system leaders, policymakers and regulators with workforce researchers. Fifteen sessions convened by leading experts combined knowledge exchange with deliberative dialogue over 2 days. Topics ranged from workforce analytics, forecasting, international migration to interprofessional working. In the small groups that were convened, important gaps were identified in both the existing research body and uptake of evidence already available. There had not been enough high quality evaluations of recent workforce initiatives implemented at pace, from virtual wards to e‐rostering. The pandemic had accelerated many changes in skillmix and professional roles with little learning from other countries and systems. Existing research was often small‐scale or focused on individual, rather than organisational solutions in areas such as staff wellbeing. In terms of existing research, managers were often unaware of accepted high quality evidence in areas like the relationship between registered nurse staffing levels and patient outcomes. More work is needed to engage new disciplines from labour economics and occupational health to academic human resources and to strengthen the emerging diverse community of healthcare workforce researchers.
Designing a model for implementing diagnosis‐related groups in Iran: An action plan approach
Authors: Asadi Farkhondeh , Anvari Mahrokh , Ghaderzadeh Mustafa , Ghorbani Nahid Ramezan 
Published: February 2024

AbstractBackground and AimsImplementing diagnosis‐related groups (DRGs) in different countries increases the efficiency of healthcare services, improves treatment quality, and reduces treatment costs. Due to the lack of a coherent model for its implementation, the present study aimed to develop a DRGs‐based implementation action plan Model for Iran.MethodsThe present study was an applied, descriptive cross‐sectional study conducted in three stages. In the first stage, a review of studies conducted in different countries was carried out. In the second stage, a model was designed for an action plan to implement the DRGs in Iran. In the third stage, the model was validated based on the Delphi technique.ResultsThe DRGs‐based implementation action plan model in Iran was designed in three primary axes, including the strategic approach of the DRGs‐based implementation action plan, technical dimensions, and executive institutions involved in the DRGs‐based implementation action plan. Validation of the designed model showed the agreement of experts (94%) for the mentioned axes.ConclusionThe significance of tailoring a DRGs‐based implementation action plan to each country's unique context is well‐established. Given the intricacies of the Iranian healthcare system, we recommend an initial pilot implementation of DRGs at the hospital level, followed by a gradual national rollout.
SARS‐CoV‐2 Omicron (BA.4, BA.5) variant: Lessons learned from a new variant during the COVID‐19 pandemic
Authors: Erabi Gisou , Faridzadeh Arezoo , Parvin Ali , Deravi Niloofar , Rahmanian Mohammad , Fathi Mobina , Aleebrahim‐Dehkordi Elahe , Rezaei Nima 
Published: February 2024

AbstractBackground and AimIn late 2021, the world faced the rapid spread of the SARS‐CoV‐2 Omicron variant, which quickly became the variant of concern. In April 2022, two new lineages of Omicron (BA.4/BA.5) emerged from Africa, where they caused the fifth wave of infection.MethodWe searched PubMed, Google Scholar, and Scopus online databases up to December 2023 for founding relevant studies.ResultsBA.4 and BA.5 subgroups, with changes in the spike protein, have a greater ability to escape from the immune system, which was possible with the help of L452R and F486V mutations. Epidemiologically, these evolving subtypes show similarities to seasonal influenza but with higher mortality rates. The symptoms of these subgroups are different from the previous types in the form of upper respiratory symptoms. Antiviral treatments, the use of antibodies such as bebtelovimab, and the development of vaccines are promising.ConclusionConsequently, we must continue to be vigilant in our joint surveillance efforts against COVID‐19 in diagnosis and treatment.
Adverse neonatal outcomes in pregnant women with asthma: An updated systematic review and meta‐analysis
Authors: Robijn Annelies L. , Harvey Soriah M. , Jensen Megan E. , Atkins Samuel , Quek Kiah J. D. , Wang Gang , Smith Hannah , Chambers Christina , Namazy Jennifer , Schatz Michael , Gibson Peter G. , Murphy Vanessa E. 
Published: February 2024

AbstractBackgroundA systematic review and meta‐analysis from 2013 reported increased risks of congenital malformations, neonatal death and neonatal hospitalization amongst infants born to women with asthma compared to infants born to mothers without asthma.ObjectiveOur objective was to update the evidence on the associations between maternal asthma and adverse neonatal outcomes.Search StrategyWe performed an English‐language MEDLINE, Embase, CINAHL, and COCHRANE search with the terms (asthma or wheeze) and (pregnan* or perinat* or obstet*).Selection CriteriaStudies published from March 2012 until September 2023 reporting at least one outcome of interest (congenital malformations, stillbirth, neonatal death, perinatal mortality, neonatal hospitalization, transient tachypnea of the newborn, respiratory distress syndrome and neonatal sepsis) in a population of women with and without asthma.Data Collection and AnalysisThe study was reported following the 2020 Preferred Reporting Items for Systematic Reviews and Meta‐Analysis (PRISMA) and the Meta‐Analysis of Observational Studies in Epidemiology (MOOSE) guidelines. Quality of individual studies was assessed by two reviewers independently using the Newcastle‐Ottawa Scale. Random effects models (≥3 studies) or fixed effect models (≤2 studies) were used with restricted maximum likelihood to calculate relative risk (RR) from prevalence data and the inverse generic variance method where adjusted odds ratios (aORs) from individual studies were combined.Main ResultsA total of 18 new studies were included, along with the 22 studies from the 2013 review. Previously observed increased risks remained for perinatal mortality (relative risk [RR] 1.14, 95% confidence interval [CI]: 1.05, 1.23 n = 16 studies; aOR 1.07, 95% CI: 0.98–1.17 n = 6), congenital malformations (RR 1.36, 95% CI: 1.32–1.40 n = 17; aOR 1.42, 95% CI: 1.38–1.47 n = 6), and neonatal hospitalization (RR 1.27, 95% CI: 1.25–1.30 n = 12; aOR 1.1, 95% CI: 1.07–1.16 n = 3) amongst infants born to mothers with asthma, while the risk for neonatal death was no longer significant (RR 1.33, 95% CI: 0.95–1.84 n = 8). Previously reported non‐significant risks for major congenital malformations (RR1.18, 95% CI: 1.15–1.21; aOR 1.20, 95% CI: 1.15‐1.26 n = 3) and respiratory distress syndrome (RR 1.25, 95% CI: 1.17–1.34 n = 4; aOR 1.09, 95% CI: 1.01–1.18 n = 2) reached statistical significance.ConclusionsHealthcare professionals should remain aware of the increased risks to neonates being born to mothers with asthma.
Aloe vera‐based biomaterial ink for 3D bioprinting of wound dressing constructs
Authors: Ceballos‐Santa M. Camila , Sierra Alfonso , Zalbidea Iker Martinez , Lazarus Emily , Marin‐Montealegre Valeria , Ramesh Srikanthan , Iglesias Patricia , Wuertz‐Kozak Karin , Rivero Iris V. 
Published: February 2024

AbstractThis study emphasizes the development of a multifunctional biomaterial ink for wound healing constructs. The biomaterial ink benefits from Aloe vera's intrinsic biocompatible, biodegradable, antioxidant, antimicrobial, anti‐inflammatory, and immunomodulatory attributes, thus alleviating the need for supplementary substances employed to combat infections and stimulate tissue regeneration. Moreover, this biomaterial ink seeks to address the scarcity of standardized printable materials possessing adequate biocompatibility and physicochemical properties, which hinder its widespread clinical adoption. The biomaterial ink was synthesized via ionic crosslinking to enhance its rheological and mechanical characteristics. The findings revealed that Aloe vera substantially boosted the hydrogel's viscoelastic behavior, enabling superior compressive modulus and the extrusion of fine filaments. The bioprinted constructs exhibited desirable resolution and mechanical strength while displaying a porous microstructure analogous to the native extracellular matrix. Biological response demonstrated no detrimental impact on stem cell viability upon exposure to the biomaterial ink, as confirmed by live/dead assays. These outcomes validate the potential of the developed biomaterial ink as a resource for the bioprinting of wound dressings that effectively foster cellular proliferation, thereby promoting enhanced wound healing by leveraging Aloe vera's inherent properties.
Preparation of catalyst of cobalt‐ and/or iron‐doped strontium zirconate and its performance in removal of m‐cresol using ozone as oxidant
Authors: Liu Weijun , Wang Ankang , Li Xunchen , Qi Meirong , Li Fuyuan , Zhao Liuqian , Chen Xiaofei , Lei Yihan , Ma Lei 
Published: February 2024

AbstractBACKGROUNDWastewater containing m‐cresol has become a global water environmental problem because its degradation is difficult, and it is highly toxic to plants, animals, and humans. The heterogeneous catalytic ozonation process has been widely used in the treatment of environmental pollution, so it is very important to study efficient catalysts. Perovskite catalysts have been widely investigated and applied in catalytic ozonation processes. Among them, strontium zirconate is a promising perovskite catalyst owing to its high oxidation efficiency and environmental friendliness.RESULTSStrontium zirconate was successfully synthesized via the co‐precipitation method. To improve strontium zirconate catalytic performance, some methods such as changing the doping ratio of cobalt and iron, changing the amount of polyethylene glycol added, changing the calcination temperature, and changing the aging time were carried out during the synthesis process. Finally, the optimal conditions were determined. 0.4 g L−1 Sr2Co0.4Fe0.6ZrO6 prepared under the optimal conditions was used for catalytic ozonation of 100 ppm m‐cresol, which achieved the optimum degradation (0.136 min−1), m‐cresol conversion rate (92.2%) and total organic carbon removal rate (12.1%) within 20 min.CONCLUSIONThis work provides the best preparation conditions. The Sr2Co0.4Fe0.6ZrO6 catalyst, as prepared, is the optimal catalyst for catalytic ozonation of m‐cresol among the investigated Sr2CoxFe1−xZrO6 catalysts. © 2024 Society of Chemical Industry (SCI).
Dental Service Organization affiliation: Understanding key aspects of State Dental Practice Act compliance
Authors: Cameron Cheryl A. , DeVore Cheryl H. 
Published: February 2024

AbstractDental Service Organizations (DSOs) are an increasingly visible and available practice option for new dental graduates. While guidance has been published to help dental students make informed decisions when considering a DSO affiliation, they have not focused on the complexities of assessing compliance with controlling state laws. Accordingly, this Perspectives article provides a concise summary of the common components of state regulatory provisions across the United States to support an understanding of the corporate practice of dentistry and compliance considerations. The guiding principles to consider include ownership or proprietorship of and control over a dental practice; control over dental offices, equipment, and materials; employment of dental personnel; and control over clinical judgment. This article should be helpful to students who are considering a DSO affiliation and educators who prepare them to enter dental practice.
Fatal cervical myelopathy in a child with glutaric aciduria type 1
Authors: Chauvet Eline , Ribeiro Diana , Kern Ilse , Fluss Joel 
Published: February 2024

AbstractWe report the case of a Syrian female refugee with late diagnosis of glutaric aciduria type 1 characterised by massive axial hypotonia and quadriplegia who only started adequate diet upon arrival in Switzerland at the age of 4 years, after a strenuous migration journey. Soon after arrival, she died from an unexpected severe upper cervical myelopathy, heralded by acute respiratory distress after a viral infection. This was likely due to repeated strains on her hypotonic neck and precipitated by an orthotopic os odontoideum who led to atlanto‐axial subluxation. This case reminds us not to omit handling patients with insufficient postural control and hypotonia with great care to avoid progressive cervical myelopathy.
Histopathological findings from the investigation of paediatric acute hepatitis of unknown aetiology, United Kingdom 2022
Authors: Callaby Helen , McGuire Emma , Rajwal Sanjay , Stahlschmidt Jens , Hartley Jane , Brown Rachel M. , Deheragoda Maesha , Quaglia Alberto , Rampling Tommy , Houlihan Catherine F. , Claire Gordon N. , Brown Colin S. , Simmons Ruth , Samson Anda , Mandal Sema , Grammatikopoulos Tassos , Demirjian Alicia 
Published: February 2024

AbstractIn 2022, there were global reports of increased numbers of acute hepatitis not explained by hepatitis A–E virus infection in children. This manuscript summarises histopathology results from 20 patients in the United Kingdom who underwent liver transplant or had a liver biopsy as part of aetiological investigations. All available histopathological samples were reviewed centrally as part of the outbreak investigation. A working group comprised of infection specialists, hepatologists and histopathologists met virtually to review the cases, presentation, investigations and histopathology. All 20 liver samples had evidence of inflammation without significant interface activity, and submassive confluent pan‐lobular or multilobular hepatocellular necrosis. Overall, the predominant histopathological findings were of acute nonspecific hepatitis with submassive hepatic necrosis and central vein perivenulitis and endothelitis. Histopathological findings were a poor indicator of aetiology.
Nitrogen fertilization rates in a subtropical peach orchard: Effect on fruit nutritional value and flavor
Authors: Ames Zilfina Rubio , Brecht Jeffrey K. , Olmstead Mercy A. , Tieman Denise , Sims Charles A. 
Published: February 2024

AbstractBackgroundThe necessity to help farmers improve yields has resulted in many years of agricultural research focused on productivity and disease resistance, neglecting other areas of fruit quality such as flavor, health benefits, and external appearance. Nitrogen is required for several biochemical processes. However, reducing N fertilization can increase the synthesis of antioxidants and volatile aroma compounds. Four‐N rates (0 (N0), 45 (N1), 90 (N2), 179 (N3), and 269 (N4) kg ha−1) were tested each year from 2011 to 2017 in two peach varieties melting flesh (MF) ‘TropicBeauty’ (TB), a soft texture peach, and non‐melting flesh (NMF) ‘UFSharp’ (UFS), a crispy texture peach, to determine the effect of N on nutritional value and flavor.ResultsThe phytochemical composition of the NMF ‘UFSharp’ (UFS) and MF variety ‘TropicBeauty’ (TB) were not cleared affected by N rates. Volatile synthesis was little affected by N. The sensory evaluation showed that consumers preferred MF peaches compared with NMF, because of its juiciness.ConclusionsNitrogen fertilization had minor effects on peach fruit phytochemical composition, volatile aroma compounds, and consumer acceptability. The N effect could had been influence by pruning practices, training of the orchard, and the delay of fruit developmental period.
Partial replacement of saturated fats in liver pâté by an olive oil‐in‐water emulsion containing β‐glucan shows no compromise in sensory and storage oxidation of lipids and protein
Authors: Zając Marzena , Kasprzak Mirosław M , Tkaczewska Joanna , Berski Wiktor , Stępień Anna , Okpala Charles Odilichukwu R , Domagała Jacek 
Published: February 2024

AbstractBACKGROUNDThe consumption of olive oil has been shown to have a positive effect on preventing obesity and hypertension. At the same time, it is recommended to avoid processed meat products as they contain saturated fats. The inclusion of highly unsaturated lipids in food products can lead to rapid oxidation and deterioration of sensory characteristics. The objective of the current work was to encapsulate olive oil and incorporate it into traditional Polish liver pâté. The oil‐in‐water emulsions were formulated with varying levels of oat β‐glucan and were evaluated for droplet size, pH, encapsulation efficiency and rheology. The liver pâtés made using the emulsions with and without β‐glucan were then evaluated for pH, texture, colour, lipid and protein oxidation, thermal stability and sensory properties.RESULTSThe results showed that the oil‐in‐water emulsions had a 100% encapsulation rate of olive oil after 30 days of storage at 4 °C, regardless of the presence of β‐glucan. Although the texture of the emulsion‐enriched liver pâté was different from that of the control, this difference was reduced when β‐glucan was added to the emulsion and then to the pâté matrix.CONCLUSIONReplacing 50% of animal fat with an olive oil emulsion enriched with β‐glucan did not result in any compromise of sensory properties, increase lipid or protein oxidation. These results suggest that it is possible to replace saturated lipids with omega‐3‐rich olive oil. © 2024 Society of Chemical Industry.
Effect of Dietary Protein Intake from Different Sources on Maternal and Umbilical Cord Plasma Amino Acid Levels
Authors: Zhao Rui , Wang Xinzheng , Liu Hongjuan , Wang Shanshan , Zhou Leilei , Cui Ningning , Guo Shu , Xiong Guoping , Yang Xuefeng , Xiong Ting , Hao Liping 
Published: February 2024

ScopeTo assess the associations of dietary protein intake from different sources during pregnancy with maternal and umbilical cord plasma amino acid levels.Methods and resultsThe study includes 216 pregnant women and 39 newborns from the Tongji Birth Cohort in Wuhan, China. The study examines the levels of 21 amino acids in maternal and cord plasma samples using ultra‐performance liquid chromatography with tandem mass spectrometry. A significant positive relationship is observed between dietary protein intake from refined grains and maternal plasma cysteine levels. Dietary protein intake from dairy products is positively associated with maternal plasma levels of sulfur amino acid (mainly cystine), but negatively associated with maternal plasma levels of glutamic acid. In addition, the study observes that pre‐pregnancy body mass index and parity may be potential determinants of maternal plasma amino acid levels, whereas a history of passive smoking during pregnancy is an important factor influencing cord plasma amino acid levels.ConclusionsThese findings suggest that dietary protein intakes from specific sources during pregnancy may affect maternal plasma levels of amino acids.
Folate Deficiency and/or the Genetic Variant Mthfr677C >T Can Drive Hepatic Fibrosis or Steatosis in Mice, in a Sex‐Specific Manner
Authors: Leclerc Daniel , Christensen Karen E. , Reagan Alaina M. , Keser Vafa , Luan Yan , Malysheva Olga V. , Wasek Brandi , Bottiglieri Teodoro , Caudill Marie A. , Howell Gareth R. , Rozen Rima 
Published: February 2024

ScopeDisturbances in one‐carbon metabolism contribute to nonalcoholic fatty liver disease (NAFLD) which encompasses steatosis, steatohepatitis, fibrosis, and cirrhosis. The goal is to examine impact of folate deficiency and the Mthfr677C >T variant on NAFLD.Methods and resultsThis study uses the new Mthfr677C >T mouse model for the human MTHFR677C >T variant. Mthfr677CC and Mthfr677TT mice were fed control diet (CD) or folate‐deficient (FD) diets for 4 months. FD and Mthfr677TT alter choline/methyl metabolites in liver and/or plasma (decreased S‐adenosylmethionine (SAM):S‐adenosylhomocysteine (SAH) ratio, methyltetrahydrofolate, and betaine; increased homocysteine [Hcy]). FD, with contribution from Mthfr677TT, provokes fibrosis in males. Studies of normal livers reveal alterations in plasma markers and gene expression that suggest an underlying predisposition to fibrosis induced by FD and/or Mthfr677TT in males. These changes are absent or reverse in females, consistent with the sex disparity of fibrosis. Sex‐based differences in methylation potential, betaine, sphingomyelin, and trimethylamine‐N‐oxide (TMAO) levels may prevent fibrogenesis in females. In contrast, Mthfr677TT alters choline metabolism, dysregulates expression of lipid metabolism genes, and promotes steatosis in females.ConclusionThis study suggests that folate deficiency predisposes males to fibrosis, which is exacerbated by Mthfr677TT, whereas Mthfr677TT predisposes females to steatosis, and reveal novel contributory mechanisms for these NAFLD‐related disorders.