bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple method comparison: Statistical model using percentage similarity
Authors: Scott Lesley E. , Galpin Jacky S. , Glencross Deborah K. 
Published: June 2003

AbstractBACKGROUNDMethod comparison typically determines how well two methods agree. This is usually performed using the difference plot model, which measures absolute differences between two methods. This is often not applicable to data with wide ranges of absolute values. An alternative model is introduced that simplifies comparisons specifically for multiple methods compared to a gold standard.METHODSThe average between a new method and the gold standard is represented as a percentage of the gold standard. This is interpreted as a percentage similarity value and accommodates wide ranges of data. The representation of the percentage similarity values in a histogram format highlights the accuracy and precision of several compared methods to a gold standard. The calculation of a coefficient of variation further defines agreement between methods.RESULTSPercentage similarity histograms of several new methods can be compared to a gold standard simultaneously, and the comparison easily visualized through use of a single 100% similarity reference line drawn common to all plots.CONCLUSIONThis simple method of comparison would be particularly useful for multiple method comparison and is especially applicable for centers collating for external quality assessment or assurance programs to demonstrate differences in results between laboratories. Cytometry Part B (Clin. Cytometry) 54B:46–53, 2003. © 2003 Wiley‐Liss, Inc.
Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases
Authors: Braun Ruedi K. , Foerster Martin , Grahmann P. Reinhard , Haefner Daniela , Workalemahu Grefachew , Kroegel Claus 
Published: June 2003

AbstractBackgroundThe integrin CD103 is preferentially expressed on intraepithelial T lymphocytes, and cells expressing this integrin may play a regulatory role in the microenvironment of the epithelial cell layer.MethodsThe relative number of CD103+/CD4+ T cells in the bronchoalveolar lavage was significantly elevated in all patients diagnosed with interstitial lung diseases compared with patients with other non‐fibrotic disorders of the lung.ResultsAnalysis by flow cytometry showed that the CD103+ and the CD103− subpopulations were memory T cells based on the high expression of CD45RO+. However, the CD103+/CD4+ T cells were CD25low, CD27−, CD28low, and CD62L−, whereas the CD103−/CD4+ T cells expressed CD25 and CD62L and were CD27high and CD28high. In addition, the CD103+/CD4+ T cells expressed significantly higher quantities of VLA‐1 and CD101 than did CD103−/CD4+ T cells. Reverse transcriptase polymerase chain reaction analysis of purified CD103+ and CD103− CD4+ T cells showed production of tumor necrosis factor (TNF) α‐R‐1 (p55), TNF‐α‐R‐2 (p75), interferon γ, interleukin‐10, and TNF‐α mRNA in both subpopulations. No interleukin‐4 mRNA was detected in either subpopulation.ConclusionsCD103+/CD4+ T cells represent a T‐helper 1–like subpopulation in human lungs with a distinct effector phenotype. Despite the lack of CD27 and the low CD25 and CD28 expression, these cells show a high degree of activation. These results suggest that CD103 expressing CD4 T cells in the lung are continuously activated, long‐living cells. Cytometry Part B (Clin. Cytometry) 54B:19–27, 2003. © 2003 Wiley‐Liss, Inc.
Flow cytometric HLA‐B27 screening: Cross‐reactivity patterns of commercially available anti–HLA‐B27 monoclonal antibodies with other HLA‐B antigens
Authors: Levering Wilfried H. B. M. , Wind Henk , Sintnicolaas Kees , Hooijkaas Herbert , Gratama Jan W. 
Published: June 2003

AbstractBackgroundSome 50 clinical laboratories in the Benelux perform flow cytometric HLA‐B27 screening and participate in the Benelux external quality assessment scheme operational since 1995. Results from this scheme indicate that cross‐reactivity of HLA‐B27 monoclonal antibodies (mAbs) is a major problem.MethodsWe analyzed cross‐reactivity patterns of commercially available mAbs for HLA‐B27 screening. Three clones of HLA‐B27 mAb (ABC‐m3, n = 3; FD705; and GS145.2) from five manufacturers were evaluated. Test cells were selected as to express HLA‐B antigens with known serologic cross‐reactions (HLA‐B7, B12, B13, B16, B17, B22, B37, B40, B41, B42, B47, and B48). Cells without B27 cross‐reactive antigens (B5, B8, B14, B15, B21, and B35) and cells positive for B27 were included as controls. All tests were performed and interpreted as recommended by the manufacturers. Cross‐reactivity was defined as increased fluorescence intensity in comparison with the baseline reactivity observed with the corresponding immunoglobulin G isotype control mAb.Results and ConclusionsAll mAbs tested showed cross‐reactivity, ranging from weak (±) to strong (+), with different antigens and different degrees of intensity—ABC‐m3: (±) B12, B16, B17, B41, B47, and B48 and (+) B7, B13, B22, B37, B40, and B42; GS145.2: (±) B13, B17, B22, B40, and B47 and (+) B7, B16, B37, B42, and B48; FD705: (±) B12, B13, B16, and B48 and (+) B17, B37, and B47. If one mAb had been used for HLA‐B27 screening, ABC‐m3 would have yielded nine false‐positive B27 assignments, FD705 would have yielded seven, and GS145.2 would have yielded two. This problem largely canbe avoided by the combined use of two different mAb clones. The combination of FD705 and GS145.2 yielded the best results, with one false‐positive HLA‐B27 assignment among the 99 HLA‐B27− samples of this highly selected panel. Cytometry Part B (Clin. Cytometry) 54B:28–38, 2003. © 2003 Wiley‐Liss, Inc.
Flow cytometry in the bone marrow staging of mature B‐cell neoplasms
Authors: Stacchini A. , Demurtas A. , Godio L. , Martini G. , Antinoro V. , Palestro G. 
Published: June 2003

AbstractBackgroundEven though flow cytometric (FC) analysis of bone marrow aspirates is often performed in hematolymphoid disorders at diagnosis and during disease monitoring, its role has not been defined during the staging of B‐non–Hodgkin's lymphoma (B‐NHL) and B‐cell lymphoproliferative diseases. The goal of this study was to provide an objective evaluation of how FC might help in the detection of bone marrow involvement by the different types of B‐cell malignant neoplasms.MethodsFifty‐four staging and 156 restaging bone marrow biopsies and bone marrow aspirates, obtained from 185 consecutive patients, were analyzed retrospectively. The results of the morphologic examination and FC were reviewed independently, and their ability to detect bone marrow involvement was compared.ResultsFC and morphology agreed in 176 cases (83.8%), i.e., both showed 77 positive cases and 99 negative ones. Discrepant results were obtained in 30 cases (14.2%) in which morphologic examination showed 25 (11.9%) positive cases, whereas FC showed no evidence of disease. FC detected involvement in five cases (2.4%) in the presence of a histologically negative bone marrow biopsy. All morphologically undetermined bone marrow cases (four) were negative by FC.ConclusionsNeither morphologic examination nor FC alone is adequate for the detection of all cases of B‐lymphoid neoplasm bone marrow involvement. FC failed to detect bone marrow involvement in those B‐NHL cases having focal paratrabecular infiltration, but proved to be more sensitive than histology in detecting small clonal B‐cells in B‐NHL, which demonstrated fewer than 5% neoplastic infiltrates. The clinical relevance of minimal disease detected by FC alone needs further evaluation because staging of lymphomas currently is based only on morphologic data. Cytometry Part B (Clin. Cytometry) 54B:10–18, 2003. © 2003 Wiley‐Liss, Inc.
Intravascular papillary endothelial hyperplasia of the neck masquerading as malignancy on fine‐needle aspiration cytology
Authors: Suh Kwang‐Sun , Shin Kyeong‐Suk , Park In‐Ae 
Published: June 2003

AbstractPapillary endothelial hyperplasia (PEH) is an exuberant, usually intravascular endothelial proliferation that, in many respects, mimics angiosarcoma. A case of PEH originally suggestive of embryonal carcinoma by fine‐needle aspiration is presented. A 12‐year‐old boy presented with a palpable mass on the right side of the neck. The mass was subsequently aspirated. Cytopathologic features showed cohesive sheets of polygonal pleomorphic cells with vesicular nuclei and prominent multiple nucleoli in a hemorrhagic background. Cytologic findings were strongly suggestive of metastatic embryonal carcinoma. There was no evidence of a primary lesion. After the mass was surgically excised, the pathologic findings showed PEH. A retrospective immunocytochemical stain for factor VIII‐related antigen on a destained ethanol‐fixed smear confirmed the endothelial nature of the polygonal cells. A vascular lesion should be considered, especially when atypical polygonal cells in a hemorrhagic background are present, as they were in this case. Diagn. Cytopathol. 2003;29:14–17. © 2003 Wiley‐Liss, Inc.
Cervical cancer screening program of Paraná: Cost‐effective model in a developing country
Authors: Bleggi Torres Luiz F. , Werner Bettina , Totsugui Joel , Collaço Luiz M. , Araújo Samuel Régis , Huçulak Márcia , Boza Edwin Javier , Fischer Rose Mari , de Laat Lucinelli , Sobbania Luiz Carlos , Raggio Armando 
Published: June 2003

AbstractThe purpose of this study is to report the organization of a cost‐effective screening program for cervical cancer in a developing country such as Brazil. The Cervical Cancer Screening Program of Paraná (CCSPP) was launched in October 1997 and was the result of a joint collaboration between the government of Paraná (Secretary of Health of the State of Paraná), scientific societies (pathologists, gynecologists, and nurses), and a nongovernmental organization called the Women's Popular Forum of Paraná. The main goal of the program was to enhance the Papanicolaou (Pap) smear screening to coverage up to 85% of female adult population with a 3‐yr interval between examinations, as well as to reduce the incidence and mortality from cervical cancer in the state of Paraná, a Southern state of Brazil. The cytological findings in all Pap smears recorded in a central computer‐based register during 5 yr of the program (October 1997–October 2002) are discussed. During that period, 2,244,158 Pap smears were performed in women included in the program from the 398 cities of the state of Paraná. The cytological smears were analyzed according to the Bethesda System. The previous year, before the program was launched, a Pap smear was taken from 43% of women of Paraná. At the end of 5 yr, coverage was increased to around 86%. The great majority of examinations had a negative result (98%). Only 2% of examinations had cytological abnormalities (n = 44,621). Low‐grade lesions predominated in women aged 15–30 yr, and the high‐grade lesions were more common in women aged 25–45 yr. Patients older than 40 yr had the greatest incidence of invasive cancer. Although the program is only 5 yr old, a decrease in the mortality from cervical cancer in women from Paraná is clearly apparent: in 1998, 297 women died of cervical cancer, as compared with 188 as of September 2002. Diagn. Cytopathol. 2003;29:49–54. © 2003 Wiley‐Liss, Inc.
Parachordoma or chordoma periphericum? Case report of a tumor of the thoracic wall
Authors: Tong Guoxia , Perle Mary Ann , Desai Panna , Kumar Asok , Waisman Jerry 
Published: June 2003

AbstractWe report the findings from an aspiration biopsy and resection of a chordoma‐like tumorous mass in the wall of the thorax of a 36‐yr‐old man with immunohistochemical, ultrastructural, and cytogenetic studies. The 4‐cm oval tumor was an incidental finding on physical examination, and no other lesions were identified after comprehensive radiologic studies. The aspirate was composed of sheets and nests of cells with distinct borders in a myxoid and fibrillary extracellular matrix. The neoplastic cells were uniform and round or polygonal with abundant pale blue vacuolated cytoplasm and small round, central or eccentric nuclei. On electron microscopy, mitochondrial rough endoplasmic reticulum complexes were seen in neoplastic cells. These features were similar to those of a conventional chordoma. However, the cytogenetic pattern, 43,XY,‐1,‐2,der (5)t(1p;5q),‐6,add(8p),add(10q), was not typical. In addition, the neoplastic cells were positive for vimentin, S‐100, AE1/AE3, CAM 5.2, and CK 19; were focally positive for EMA and smooth muscle actin; and were negative for cytokeratin 1 and 10 (34βE12), CK 7, CK 8 (35H 11B), CK 17, and CK 20. The cytogenetic and immunohistochemical patterns were different from conventional chordoma and its peripheral counterpart, chordoma periphericum, suggesting the diagnosis of parachordoma. To the best of our knowledge, this is the first report of fine‐needle aspiration of this newly defined and rare entity. Diagn. Cytopathol. 2003;29:18–23. © 2003 Wiley‐Liss, Inc.
Cytologic features of small‐cell carcinoma on ThinPrep®
Authors: Bavikatty Neil R. , Michael Claire W. 
Published: June 2003

AbstractThe use of ThinPrep® (TP) technology for fine‐needle aspiration (FNA) cytology has become widely accepted. However, some literature suggests that small‐cell carcinoma may present a diagnostic pitfall due to morphologic alterations. In this study, we retrospectively compared 14 FNA of small‐cell carcinoma prepared using TP with corresponding conventional smears (CS). We also examined the TP appearance of 23 other small round‐cell lesions in order to determine if differential diagnostic features were preserved. TP and CS were evaluated semiquantitatively for background, architecture, chromatin quality, nuclear molding, nuclear smearing, nucleolar prominence, amount of cytoplasm, nuclear size, and single‐cell necrosis. The data were analyzed using the McNemar χ2 test.TP slides of small‐cell carcinoma showed a cleaner background than CS (P < 0.005). Although some degree of nuclear molding was preserved, it was decreased in amount (P < 0.025) and subtler in quality. Similarly, nuclear smearing was present but decreased in amount (P < 0.05), and less prominent qualitatively. The amount of discernible cytoplasm was greater on TP (P < 0.005). No significant differences were found for any of the other parameters studied. The presence of nuclear molding was the single most useful feature in differentiating small‐cell carcinoma from other small round‐cell tumors on TP.Small‐cell carcinoma may be diagnosed with confidence by FNA using TP. However, pathologists should be aware of certain morphologic alterations in order to avoid diagnostic pitfalls. Diagn. Cytopathol. 2003;29:8–12. © 2003 Wiley‐Liss, Inc.
Pelvic washing cytology of ovarian Sertoli‐Leydig‐cell tumor with retiform pattern: A case report
Authors: Guo Meihua , Lim Jennifer C. , Wojcik Eva M. 
Published: June 2003

AbstractA Sertoli‐Leydig‐cell tumor is an exceptionally rare neoplasm. We present the pelvic washing cytomorphology of an ovarian Sertoli‐Leydig‐cell tumor with a retiform pattern in a 24‐yr‐old female. The cytologic features in this case were tight tissue fragments composed of small, relatively uniform cells with scanty cytoplasm and small rounded or blunt papillary fragments with hyalinized cores lined with small, mildly atypical cuboidal cells. Differential diagnoses included borderline and well‐differentiated papillary serous tumors, clear‐cell carcinoma, and collagen balls. Correlation of cytologic findings with histomorphology is crucial for correct interpretation of pelvic washings in cases of Sertoli‐Leydig‐cell tumors. Diagn. Cytopathol. 2003;29:28–30. © 2003 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of intraabdominal extralobar pulmonary sequestration: A case report
Authors: Rajendiran Swaminathan , Kapoor Vibhu , Schoedel Karen 
Published: June 2003

AbstractA 55‐yr‐old female presented with a mass near the left adrenal gland detected on a CT scan performed for breast cancer staging. A CT‐guided fine‐needle aspiration (FNA) was performed and showed scattered ciliated cells, compatible with benign respiratory‐type epithelial cells. CT‐guided needle biopsy also showed fibrocollagenous and fibroadipose connective tissue with focal respiratory type epithelium with subepithelial mucus glands. A final diagnosis of extralobar pulmonary sequestration (ELS) was rendered after surgical resection. This uncommon benign congenital malformation should be considered in the differential diagnosis of FNA of an intraabdominal mass in an adult. Diagn. Cytopathol. 2003;29:24–27. © 2003 Wiley‐Liss, Inc.
Reporting of atypical squamous cells, cannot exclude a high‐grade squamous intraepithelial lesion (ASC‐H) on cervical samples: Is it significant?
Authors: Selvaggi Suzanne M. 
Published: June 2003

Abstract“Atypical squamous cells, cannot exclude a high‐grade squamous intraepithelial lesion (ASC‐H)” is a new diagnostic category in the 2001 Bethesda nomenclature system for cervical cytology. The purpose of this 7‐mo retrospective study (March 1, 2002–September 30, 2002) was to evaluate the significance of ASC‐H on cervical Thin Prep® Pap Tests™. During this period, 25 (0.27%) of 9,214 Pap Tests were diagnosed as ASC‐H, 22 of which resulted in either follow‐up cervical biopsies and/or cervical cones, and which formed the basis of this study. Tissue specimens (22 cases) were negative in 5 cases (23%) and positive in 17 cases (77%). Of the positive specimens, there were 2 (12%) low‐grade squamous intraepithelial lesions (LSIL) and 15 (88%) high‐grade squamous intraepithelial lesions (HSIL). Of the 22 cases, ASC‐H diagnoses included immature/atypical squamous metaplasia vs. a squamous intraepithelial lesion (SIL) in 19 (86%) cases, and tight clusters of small cells with a high nuclear to cytoplasmic ratio in 3 (14%) cases. The results of this study indicate that the reporting of ASC‐H on cervical samples does lead to the detection of HSILs in a significant number of cases (68% in this study). Therefore, further evaluation of the patient is warranted. Diagn. Cytopathol. 2003;29:38–41. © 2003 Wiley‐Liss, Inc.
Incidental detection of microfilariae in aspirates from Ewing's sarcoma of bone
Authors: Ahluwalia Charanjeet , Choudhury Monisha , Bajaj P. 
Published: June 2003

AbstractMicrofilariae have been reported as coincidental findings with various neoplasms. We present a case in which microfilariae were encountered in fine‐needle aspiration (FNA) smears from a 10‐yr‐old boy who presented with a lytic lesion in the diaphysis of the right tibial bone. Stained aspirates from the swelling were composed of a dispersed population of small round cells with scanty to vacuolated cytoplasm. Two sheathed microfilariae with several nuclei and cephalic and caudal clearing were identified. A diagnosis of Ewing's sarcoma with presence of microfilariae of Wuchereria bancrofti was made. The diagnosis of Ewing's sarcoma was confirmed on histopathology. Diagn. Cytopathol. 2003;29:31–32. © 2003 Wiley‐Liss, Inc.
The place of fine‐needle aspiration in the preoperative diagnosis of the congenital sublingual teratoid cyst
Authors: Babuccu Orhan , Işiksaçan Özen Özlem , Hoşnuter Mübin , Kargi Eksal , Babuccu Berfu 
Published: June 2003

AbstractThe term “teratoid cyst” was first used by Meyer in his classification of dysontogenetic cysts of the cervicofacial region, which was based on the type of germinative layers included in the cyst wall. Sublingual location of a dermoid cyst is not common, with an incidence of 1.6%. The teratoid cyst is the least common, accounting for 1.8% of sublingual dermoid cysts, and such lesions are extremely rare in infancy. In our case, a 7‐mo‐old male infant was referred with a sublingual mass. Ultrasonography yielded a cystic mass with internal echoes but no specific diagnosis. The smears obtained from fine‐needle aspiration (FNA) displayed sheets of large, benign‐appearing, anucleated and nucleated squamous cells. There were a number of neutrophils, which had no significant importance. No atypical cells were observed. According to the clinical picture and FNA findings, it appeared to be a cystic structure of keratogenous origin and could have been any type of dermoid cyst. The cyst was excised completely by the oral approach. On histopathological examination, the presence of skin appendages along with mature cartilage and respiratory epithelium confirmed the final diagnosis of a teratoid cyst. In conclusion, although FNA is not comparable with computed tomography (CT) and magnetic resonance imaging (MRI), it might be valuable for the diagnosis of lesions occurring in this anatomical location. It is safe, cost‐effective, and reliable. FNA is not only able to help selecting the most appropriate surgical method, but also be used as a therapeutic modality in some emergencies or during surgery. Diagn. Cytopathol. 2003;29:33–37. © 2003 Wiley‐Liss, Inc.
Clinical impact (cost‐effectiveness) of qualifying atypical squamous cells of undeterminate significance (ASCUS) in cases favoring a reactive or dysplastic process
Authors: Carozzi F.M. , Cariaggi M.P. , Bonardi L. , Bulgaresi P. , Cecchini S. , Ciatto S. , Cipparrone I. , Galanti L. , Maddau C. , Matucci M. , Rubeca T. , Troni G.M. , Turco P. , Zappa M. , Confortini M. 
Published: June 2003

AbstractThe cost‐effectiveness of qualifying ASCUS cases into two different subcategories, favoring a reactive (ASCUS‐R) or dysplastic process (ASCUS‐S), was evaluated at the Centro per lo Studio e la Prevenzione Oncologica of Florence in a prospective study. The study determined the positive predictive value (PPV) for histologically confirmed cervical intraepithelial neoplasia grade 2 (CIN2) or more (CIN>) severe lesion of the two ASCUS subgroups. ASCUS‐S had a PPV (10.78%) comparable to low‐grade squamous intraepithelial lesions (LSIL) (11.40%). For ASCUS‐R cases, the recommendation of 6‐mo repeat cytology prompting colposcopy in cases of persistent ASCUS or more severe cytology was also effective, as it selected a subgroup with a relatively high PPV (10.34%). The cost‐effectiveness of a protocol based on ASCUS qualification was compared with two other possible options for nonqualified ASCUS cases: immediate colposcopy and colposcopy in persistent ASCUS at 6‐mo repeat cytology.. The detection rate of CIN2> was substantially higher using ASCUS qualification (35.9 vs 14.8 or 17.1). The cost per ASCUS subject was €24.99, 27.11, or 25.14 and that per CIN2> detected was €697, 1,831 or 1,470 for the three options, respectively. The evidence that ASCUS detection option implies a higher detection rate of CIN2> and subsequently a lower cost per CIN2> detection must be considered with caution and deserves confirmation by other comparative studies. Diagn. Cytopathol. 2003;29:4–7. © 2003 Wiley‐Liss, Inc.
Selective phosphorylation of dihydroquercetin with trivalent phosphorus reagents
Authors: Nifant'ev E. E. , Kukhareva T. S. , Dzgoeva Z. M. , Vasyanina L. K. , Koroteev M. P. , Kaziev G. Z. 
Published: June 2003

AbstractCharacteristic features of phosphorylation of dihydroquercetin with trivalent phosphorus reagents were studied. Preparative methods for regioselective phosphorylation were found. The most important chemical properties of dihydroquercetin phosphites and cyclophosphites were studied. © 2003 Wiley Periodicals, Inc. Heteroatom Chem 14:399–403, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.10115
Cyclo[bis(1,7‐naphthylenedialkylamidophosphites)]
Authors: Nifantyev Eduard E. , Rasadkina Elena N. , Evdokimova Yuliya B. , Stash Adam I. , Belsky Vitaly K. , Vasyanina Larisa K. 
Published: June 2003

AbstractThe possibility of regioselective cyclobisphosphorylation of nonsymmetrical bisphenols was shown for the 1,7‐dihydroxynaphthalene–phosphorous acid triamide system. The structure and essential chemical properties of the first nonsymmetrical phosphacyclophane were studied. New data demonstrating peculiar features of bisphenol diamidophosphites were obtained. © 2003 Wiley Periodicals, Inc. Heteroatom Chem 14:404–412, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.10143