bluuebunny/crossref_metadata_2025_split huggingface dataset:
A model of chronic thromboembolic pulmonary hypertension with the use of microencapsulated fibrin particles
Authors: Karpov A. A. , Shilenko L. A. , Vaulina D. D. , Sidorova E. E. , Akhmetova A. A. , Bunenkov N. S. , Vorotilov A. V. , Ivkin D. Yu. , Karpenko V. V. , Galagudza M. M. 
Published: January 2025

Aim. To develop a model of chronic thromboembolic pulmonary hypertension (CTEPH) in rats by embolization of the pulmonary vascular bed with microencapsulated fibrin (MF).Materials and methods. Microencapsulated fibrin (MF) was prepared by encapsulating fibrin particles smaller than 71 μm in sodium alginate. Non-encapsulated fibrin with a particle size of 71–200 µm was used as an alternative embolic particle. Modeling was performed on male Wistar rats. The animals were divided into 4 groups. Intact (INT) animals (n = 7) were administered normal saline intravenously. In the NF8 group (n = 14), non-encapsulated fibrin was injected as embolic particles 8 times every 4 days. In the MF5 group (n = 14), 0.5 ml MF (9,047 ± 430 particles) was administered intravenously 5 times every 5 days. In the MF8 group (n = 14), MF was administered 8 times every 4 days. Six weeks after the last injection of embolic particles, cardiac catheterization with manometry and histologic examination of the lungs were performed.Results. According to cardiac catheterization, right ventricular systolic pressure (RVSP) in the MF8 group was significantly higher compared to rats from the INT and NF8 groups (p < 0.05). The hypertrophy index and the percentage of collagen fibers in the structure of the vascular wall of the pulmonary artery branches were significantly higher in the MF5 and MF8 groups than in the INT and NF8 groups (p < 0.01). There were no significant differences between the MF5 and MF8 groups.Conclusion. A representative CTEPH model in rats was developed, characterized by a stable increase in RVSP and pronounced structural changes in the branches of the pulmonary artery.
Changes in VEGFR1 and VEGFR2 expression and endothelial cell maturity in laboratory animals with a model of Alzheimer’s disease
Authors: Kukla M. V. , Averchuk A. S. , Stavrovskaya A. V. , Rozanova N. A. , Berdnikov A. K. , Kolotyeva N. A. , Salmina A. B. 
Published: January 2025

Aim. To evaluate the expression of VEGFR1 and VEGFR2 and the maturity of endothelial cells in neurogenic niches in the model of Alzheimer’s disease.Materials and methods. The study was carried out on 6-month-old male C57BL/6 mice. The experimental group (n = 15) received 2 µl of 1 mM Aβ25-35 solution in the CA1 hippocampal region, while the control group (n = 15) received normal saline. Brain plasticity was assessed at day 10, 17, and 38 after surgery by the passive avoidance test. The expression of VEGFR1, VEGFR2, and CLDN5 was assessed by immunohistochemistry and the Image ExFluorer imaging system.Results. In the control group, cognitive training stimulated angiogenesis in the neurogenic niches of the brain, which was accompanied by the formation of microvasculature with fully mature endothelium. In the experimental group, an early and pronounced increase in the VEGFR1 expression was observed by day 7 after cognitive training, which was followed by impaired barrier formation and high VEGFR2 expression by day 28 after cognitive training. These changes were associated with the formation of small vessels with structural incompetence of endothelial cells.Conclusion. Angiogenesis in neurogenic niches of the animals with the model of Alzheimer’s disease is characterized by incompetent mechanisms regulating the subpopulation composition of endothelial cells, impaired stabilization of the endothelial layer, and a decrease in the maturation rate of endothelial cells in newly formed microvessels by the time of cognitive deficit manifestation. This may contribute to microcirculatory dysfunction and impaired neurogenesis in neurogenic niches as well as to the development of pathological permeability and neuroinflammation. On the whole, the disruption of angiogenesis in neurogenic niches observed in the animal model of Alzheimer’s disease suggests a potential contribution of this mechanism to the development of aberrant brain plasticity.
Dynamic changes in RNA integrity, gene expression, and tissue pathomorphology of experimental mice in the postmortem period
Authors: Buyko E. E. , Perina E. A. , Vasilchenko D. V. , Tsydenova I. A. , Khmelevskaya E. S. , Ufandeev A. A. , Kaidash O. A. , Ivanov V. V. , Vtorushin S. V. , Udut E. V. 
Published: January 2025

Aim. To examine the pattern of morphological changes, RNA quality number, and gene expression in mouse tissues sampled at autopsy under controlled experimental conditions.Materials and methods. Balb/c mice were euthanized and subsequently subjected to necropsy at 0, 3, 12, 24, 48, and 72 hours of the postmortem period. During the first three hours following euthanasia, the mice were maintained at room temperature, after which they were transferred to a refrigerator (4 º С). Total RNA was extracted from tissue samples taken from the kidney, liver, and brain; the integrity of the RNA samples was assessed by capillary electrophoresis, and the RNA quality number (RQN) was calculated. The expression levels of Actb, Epas1, and Rps18 housekeeping genes were evaluated by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) with original primers and probes using the TaqMan assay. The histologic examination was performed according to standard techniques.Results. Degradation of RNA extracted from mouse kidney tissues appeared to be greater than that of RNA taken from the liver. In the meantime, a negative linear correlation was observed between RQN and the duration of the postmortem interval for liver and kidney samples. In contrast, no significant changes in the RQN score were observed for brain RNA samples at any of the time points. The expression of the Epas1 and Rps18 genes was significantly decreased in mouse kidney and liver tissues. However, the level of Epas1 and Rps18 gene expression in the brain remained stable at all time points and did not exhibit a significant decrease at 72 hours after euthanasia. No obvious morphological changes were detected by the histologic examination, which does not exclude the presence of ultrastructural pathological changes.Conclusion. RQN in autopsy tissues serves as a crucial predictor of sample quality for molecular biology studies, including gene expression analysis.
Phenotypic profile of blood monocytes and tumor-associated macrophages in relation to the expression of galectins 1 and 3 in colorectal cancer
Authors: Kurnosenko A. V. , Reingardt G. V. , Poletika V. S. , Kolobovnikova Yu. V. , Chumakova S. P. , Urazova O. I. , Grishchenko M. Yu. , Churina E. G. , Gamirova K. A. 
Published: January 2025

Aim. To identify the features of the subpopulation composition of blood monocytes and tumor macrophages in relation to the plasma concentration and intratumoral expression of galectins 1 and 3 in patients with colorectal cancer.Materials and methods. A total of 23 patients with colorectal cancer (ICD C18-20) were examined – 5 men and 18 women (average age 63.8 ± 9.4 years). The control group consisted of healthy volunteers; the comparison group encompassed age- and sex-matched patients with colon adenomas. The study materials included whole blood and tumor biopsies. The concentration of galectins 1 and 3 in the blood was determined by enzyme-linked immunosorbent assay, the content of tumor galectin-1+ and galectin-3+ cells – by immunohistochemistry. Subpopulations of blood monocytes were evaluated by flow cytometry; the macrophage immunophenotypes M1 (CD68+ CD80+ ) and M2d (CD68+ CD206+ ) in tumor tissues were determined using immunofluorescence staining. Statistical processing of the research results was performed by the Jamovi 2.3.21 software package for Windows.Results. In patients with colorectal cancer (CRC), a positive relationship was identified between high plasma concentrations of galectins 1 and 3 and an imbalance of blood monocytes manifested by a decrease in the relative count of classical CD14++CD16- monocytes and, conversely, an increase in the number of non-classical CD14+CD16++ and intermediate CD14+ CD16- cells. The relative numbers of M1 (CD68+CD80+) and M2d (CD68+CD206+) macrophages in CRC tissue samples turned out to be comparable and did not depend on the level of galectins 1 and 3 in the blood and tumor. In patients with colon adenomas, the M2d subpopulation of tumorassociated macrophages was predominant (p = 0.031).Conclusion. In patients with CRC, galectins 1 and 3 have a modulating effect on the ratio of non-classical CD14+CD16++, intermediate CD14+CD16- , and classical CD14++CD16- monocytes in the blood and do not affect the M1/M2d expression profile of tumor-associated macrophages.
Predictors of mortality in hospitalized patients with COVID-19
Authors: Malinovskiy V. A. , Fedosenko S. V. , Semakin A. V. , Dirks I. I. , Arzhanik M. B. , Semenova O. L. , Vinokurova D. A. , Starovoitova E. A. , Agaeva S. A. , Nesterovich S. V. , Kalyuzhin V. V. 
Published: January 2025

Aim. To determine clinical and laboratory factors associated with a severe course and lethality in hospitalized patients with COVID-19.Materials and methods. A retrospective comparative study included data of 745 adult patients hospitalized with COVID-19 from 16.05.2020 to 30.09.2020 (Tomsk, Russia). The intergroup comparison of indices, ROC analysis, and determination of odds ratio to assess the association between risk factors and the outcome were performed.Results. Age > 62 years, pneumonia within a year before COVID-19, and the presence of ≥3 comorbidities were associated with a fatal outcome (FO). Negative predictors of the outcome at the time of hospitalization included dyspnea, diastolic blood pressure ≤ 80 and pulse pressure > 48 mmHg, SpO2 < 94% (and/or a decrease to ≤ 89% throughout hospitalization). Laboratory predictors of FO at admission were platelets ≤ 183 × 109 / l, neutrophils > 4.57 × 109 / l, lymphocytes ≤ 1.08 × 109 / l, neutrophil-to-lymphocyte ratio > 4.8, aspartate aminotransferase > 39 U / l, urea > 6.75 mmol / l, lactate dehydrogenase > 219 U / l, blood albumin ≤ 38 g / l, C-reactive protein (CRP) > 47 mg / l. When threshold values were reached during any of the hospitalization periods, FO was associated with CRP > 38 mg / l, ferritin > 648.6 µg / l, D-dimer > 731.11 ng / ml, white blood cells > 14.27 × 109 / l, lymphocytes ≤ 0.73× 109 / l, duration of oxygen therapy > 3 days, need for non-invasive and invasive ventilation ≥ 1 day, need for glucocorticoid administration > 1 day, reaching a total course dose > 6 mg for dexamethasone.Conclusion. The factors associated with FO in hospitalized patients with COVID-19 were identified.
The Existence of Women in the Pre- and Post-Reformation Period in The Novel Laut Bercerita: A Review of Feminism
Authors: Ju Roh Jung , M. Yoesoef M. Yoesoef , Setyani Turita Indah 
Published: January 2025

<p class="Abstract">Women are faced with various stereotypes of being second-class beings. The female must accept being a being who must be under the position of the male. However, in contrast to such stereotypes, literary works are able to show a deconstruction of the conditions and situations of women as empowered beings. This is illustrated through one of Indonesia's novels entitled <em>Laut Bercerita </em>by Leila S. Chudori. Women are described as figures who have a role to be able to show their existence even though at that time there was political turmoil before 1998. One of the important things that deconstructs stereotypes is the existence of strong women who are able to heal male characters through their efforts. Women who are usually used as <em>second sex </em>in patriarchal confines seek to be undermined in the narrative of the novel. Based on this, the purpose of this study is to dismantle the existence of women in the time of turmoil before and after the reformation in the novel <em>Laut Bercerita.</em> This research is a qualitative descriptive study with the approach of Simon de Beauvoir existentialist feminism. The results of this study show that women managed to get out of the notion of <em>second</em> sex by showing existence in the public domain by getting a proper education and job in the public domain, being able to struggle to find their brother's character and her brother's colleague who were victims of student disappearance in 1998, being able to cure men due to kidnapping and torture in 1998, and the struggle to get information on the development of cases of losing older siblings and his brother's partner in the period before 1998.</p>
Dengan cara apa mengajar tidak sepenuhnya dianggap profesi?
Authors: Hilman Taufik , Rusmana Nandang , Hyangsewu Pandu , Riyadi Lanang 
Published: January 2025

<p>Profesi mengajar sering kali tidak mendapatkan pengakuan dan penghargaan yang layak<strong> </strong>Artikel ini menginvestigasi faktor-faktor yang mempengaruhi persepsi terhadap profesi mengajar di Indonesia, dengan fokus pada persyaratan pendidikan, tingkat gaji, otonomi profesional, dan pandangan tradisional tentang pendidikan. Metode penelitian menggunakan tinjauan literatur untuk mengumpulkan informasi dari berbagai sumber yang relevan. Temuan menunjukkan bahwa persyaratan pendidikan dan sertifikasi guru bervariasi, dengan implemen-tasi yang tidak konsisten di seluruh negeri. Tingkat gaji guru masih tergolong rendah, sementara otonomi profesional mereka dalam pengajaran sering kali terbatas. Pandangan tradisional tentang pendidikan sering kali menganggap guru sebagai penjaga nilai-nilai moral, yang dapat mengurangi pengakuan terhadap profesionalisme mereka. Implikasi dari temuan ini menyoroti perlunya tindakan untuk meningkatkan status profesional guru di Indonesia, termasuk peningkatan gaji, pemberian otonomi yang lebih besar, dan edukasi masyarakat tentang peran penting guru dalam pembangunan bangsa. Dengan menerapkan rekomendasi yang diajukan, diharapkan dapat tercapai perbaikan dalam kualitas pendidikan dan pembangunan sumber daya manusia di Indonesia.</p><p> </p><p><strong><em>In what ways is teaching not fully considered a profession?</em></strong><strong><em></em></strong></p><p> </p><p><strong><em>Abstra</em></strong><strong><em>ct</em></strong><strong><em>: </em></strong><em>This article investigates the factors that influence perceptions of the teaching profession in Indonesia, focusing on educational requirements, salary levels, professional autonomy, and traditional views of education. The research method uses a literature review to gather information from various relevant sources. Findings show that teacher education and certification requirements vary, with inconsistent implementation across the country. Teachers' salary levels remain low, while their professional autonomy in teaching is often limited. Traditional views of education often regard teachers as guardians of moral values, which can reduce recognition of their professionalism. The implications of these findings highlight the need for action to improve the professional status of teachers in Indonesia, including increased salaries, greater autonomy, and public education about the important role teachers play in nation-building. By implementing the proposed recommendations, it is hoped that improvements in the quality of education and human resource development in Indonesia can be achieved.</em><em></em></p>
Upaya peningkatan penguasaan kosakata pada pembelajaran Bahasa Inggris melalui Bingo Games di sekolah dasar
Authors: Khasanah Fitrotul , Anjarini Titi , Ratnaningsih Arum 
Published: January 2025

<p>Penelitian ini bertujuan untuk meningkatkan penguasaan kosakata Bahasa Inggris siswa melalui penggunaan <em>Bingo Games</em> dalam pembelajaran di SDN Pageron Purworejo. Metode yang digunakan adalah Penelitian Tindakan Kelas dengan pendekatan kualitatif. Penelitian ini dilaksanakan dalam dua siklus. Subjek penelitian adalah siswa kelas 5 SDN Pageron Purworejo yang berjumlah 18 siswa. Hasil penelitian menunjukkan bahwa penggunaan <em>Bingo Games</em> terbukti signifikan dalam meningkatkan penguasaan kosakata Bahasa Inggris siswa. Data menunjukkan rata-rata peningkatan kosakata sebesar 72.92% setelah penerapan metode permainan ini selama 1 minggu. Peningkatan kosakata bervariasi antara siswa, dengan pe-ningkatan tertinggi mencapai 90.91% dan terendah 58.82%. Hal ini menunjukkan bahwa meski-pun metode ini secara umum efektif, ada perbedaan dalam dampaknya terhadap setiap siswa. Faktor-faktor seperti keterlibatan individu, motivasi, dan pemahaman awal siswa dapat mem-pengaruhi hasil. Metode permainan ini tidak hanya membantu siswa mengingat kosakata baru, tetapi juga meningkatkan keterlibatan dan motivasi siswa dalam proses pembelajaran. Selain itu, <em>Bingo Games</em> mendorong interaksi antar siswa, yang berkontribusi pada pencapaian hasil belajar yang lebih baik.</p><p> </p><p><strong><em>Efforts to improve vocabulary acquisition in English language learning through Bingo Games at elementary school</em></strong><strong><em></em></strong></p><p> </p><p><strong><em>Abstra</em></strong><strong><em>ct</em></strong><strong><em>: </em></strong><em>This research aims to improve students' mastery of English vocabulary through the use of Bingo Games in learning at SDN Pageron Purworejo. The method used is classroom action research (PTK) with a qualitative approach. This research was carried out in two cycles. The research subjects were 18 grade 5 students at SDN Pageron Purworejo. The research results showed that the use of Bingo Games proved significant in improving students' mastery of English vocabulary. Data shows an average increase in vocabulary of 72.92% after implementing this game method for 1 week. Vocabulary increases varied between students, with the highest increase reaching 90.91% and the lowest 58.82%. This shows that although these methods are generally effective, there are differences in their impact on individual students. Factors such as individual engagement, motivation, and students' prior understanding can influence outcomes. This game method not only helps students remember new vocabulary but also increases student involvement and motivation in the learning process. In addition, Bingo Games encourage interaction between students, which contributes to achieving better learning outcomes.</em><em></em></p>
Peran feedback peserta didik dalam efektivitas pembelajaran terdiferensiasi di sekolah dasar
Authors: Komara Intan Budi , Murron Faisal Sadam , Heryanto Dwi , Zaman Mochamad Faizal 
Published: January 2025

<p>Penelitian ini dilatarbelakangi pentingnya peran <em>feedback</em> peserta didik dalam meningkatkan efektivitas pembelajaran terdiferensiasi. Pada penerapan <em>feedback</em> peserta didik ini sering kali tidak optimal, terutama di tingkat sekolah dasar. Penelitian ini bertujuan untuk menganalisis peran <em>feedback</em> peserta didik yang dapat diintegrasikan secara efektif di SD, serta mengidentifikasi karakteristik peserta didik yang berbeda-beda dalam menggunakan <em>feedback</em> tersebut. Penelitian ini menggunakan metode pendekatan kualitatif dengan observasi dan wawancara. Hasil penelitian menunjukkan bahwa peran <em>feedback</em> peserta didik dapat meningkatkan pembelajaran terdiferensiasi dengan adanya respon peserta didik terhadap tindak lanjut pembelajaran, namun masih terkendala oleh keterbatasan waktu dan pemahaman guru mengenai peran <em>feedback</em> peserta didik. Penelitian ini merekomendasikan peran <em>feedback</em> peserta didik untuk mempermudah pengumpulan dan analisis kebutuhan peserta didik yang berbeda-beda, serta memberikan wawasan kepada guru untuk meningkatkan keterampilan dalam merespons <em>feedback</em> peserta didik secara efektif. Implikasi dari penelitian ini adalah dapat memberikan kontribusi pada pengembangan pendekatan pembelajaran yang lebih inklusif dan responsif terhadap kebutuhan peserta didik di kelas.</p><p> </p><p><strong>The role of student feedback in the effectiveness of differentiated learning in elementary schools</strong></p><p> </p><p><strong>Abstract</strong>: This study is motivated by the importance of the role of student feedback in improving the effectiveness of differentiated learning. The application of student feedback is often not optimal, especially at the elementary school level. This study aims to analyze the role of student feedback that can be integrated effectively in elementary schools, as well as to identify the characteristics of different students who use the feedback. This study uses a qualitative approach, using observation and interviews. The results of the study indicate that the role of student feedback can improve differentiated learning with student responses to follow-up learning, but is still constrained by time constraints and teachers' understanding of the role of student feedback. This study recommends the role of student feedback in facilitating the collection and analysis of different student needs and providing insight to teachers to improve their skills in responding to student feedback effectively. The implications of this study are that it can contribute to the development of a more inclusive and responsive learning approach to the needs of students in the classroom.</p>
Penerapan Model Pembelajaran Problem Based Learning dengan Media Konkret untuk Meningkatkan Pembelajaran Matematika tentang Bangun Ruang pada Siswa Kelas V di SD Negeri Sitibentar Tahun Ajaran 2022/2023
Authors: Hidayah Tri Nurohmatul , Salimi Moh , Wahyudi Wahyudi 
Published: January 2025

<p class="TableParagraph">Tujuan penelitian ini adalah (1) mendeskripsikan langkah-langkah penerapan model pembelajaran Problem Based Learning (PBL) dengan media konkret (2) meningkatkan pembelajaran Matematika tentang bangun ruang pada siswa kelas V melalui penerapan model <em>Problem Based Learning </em>dengan media konkret. (3) mendeskripsikan kendala dan solusi pada penerapan model Problem Based Learning (PBL) dengan media konkret. Penelitian tindakan kelas ini dilaksanakan dalam tiga siklus. Subjek penelitian ini ialah guru kelas dan siswa kelas V SDN Sitibentar. Data yang digunakan berupa data kualitatif dan kuantitatif. Teknik pengumpulan data menggunakan observasi, wawancara, studi dokumen dan tes. Validitas data menggunakan triangulasi sumber dan teknik. Analisis data dilaksanakan melalui reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian menunjukkan terjadi peningkatan pembelajaran Matematika tentang bangun ruang dengan model <em>Problem Based Learning </em>dengan media konkret, mendeskripsikan kendala dan solusi pada penerapan <em>Model Problem Based Learning</em>. Presentase ketuntasan hasil belajar matematika mengalami pada siklus I = 75,5%, siklus II = 80,5%, siklus III = 89,5% Dapat disimpulkan bahwa penerapan model <em>Problem Based Learning </em>dengan media konkret dapat meningkatkan pembelajaran Matematika tentang bangun ruang pada siswa kelas V SDN Sitibentar tahun ajaran 2022/2023.</p>