bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotypic plasticity mediates colorectal cancer metastasis
Authors: Efremova Mirjana , Ogden Samuel , Metic Nasrine , Smith Elise , Berner Alison , Baker Ann-Marie , Uddin Imran , Chelala Claude , Ullah Dayem , Saad Amina , Aleong Jo-Anne Chin , Graham Trevor , Kocher Hemant 
Published: February 2024

Abstract
A major challenge for inhibiting metastasis is the ability of cancer cells to reversibly switch states in response to microenvironmental cues along the metastatic cascade. The regulatory factors and signals from the microenvironment enabling colorectal cancer (CRC) cells to transition into an invasive state and to establish metastasis in the liver remain unknown. Using a combination of single-cell multiomics and spatial transcriptomics data from primary and metastatic CRC patients, we reveal putative metastasis-initiating cancer states with regenerative and inflammatory signatures, driven by transcription factors AP-1, NF-κB and YAP. We demonstrate the existence of an intermediate population with a hybrid regenerative and stem phenotype, indicating phenotypic transitions between stem and pro-metastatic cells. Our spatial analyses show localisation of the regenerative states at the invasive edge in primary CRC and in an immunosuppressive niche in liver metastasis, surrounded by immune and stromal cells that sustain these cells. We uncover putative ligand-receptor interactions driven by cancer-associated fibroblasts (CAFs), macrophages and CD8 T cells that activate the regenerative and inflammatory invasive phenotype in cancer cells. Together, our findings reveal regulatory and signalling factors that can be targeted to restrict transition into invasive states to impair metastasis.
Adaptive changes of ROS/RNS redox and melatonin synthesis under salt and waterlogging stresses in Pittosporum tobira
Authors: Pan Xiaojiao , Wang Pengcheng , Teng Mingjun , Bao Manzhu 
Published: February 2024

Abstract
Plants have evolved a variety of complex mechanisms to resist the environmental factors including salt and waterlogging stresses. In this study, we described adaptive changes of Pittosporum tobira toward salt and/or waterlogging stresses by mediating ROS (reactive oxygen species)/RNS (reactive nitrogen species) redox and melatonin synthesis. When the P. tobira seedlings were subjected to salt stress, waterlogging stress, and salt-waterlogging stress, both the photosynthetic capacity and antioxidant capacity were significantly inhibited, accompanying with the alterations of MDA, H2O2, O2− and NO levels and melatonin metabolism. These observations were correlated with the changes in the activities of antioxidant enzymes (SOD, CAT, POD and APX) and melatonin biosynthetic enzymes (MEL, TDC, SNAT, SER, and 5-MT) as wells as in the expression of their encoding genes. Lower melatonin content was found in the seedlings treated by salt-waterlogging stress than in those treated by salt or waterlogging stress. Furthermore, the tolerances of the seedlings grown at Zhejiang province to salt and waterlogging stress were stronger than those grown at Fujian province. Our findings suggested that the MEL/ROS/RNS redox network induced by salt stress, waterlogging stress, salt-waterlogging stress may be a crucial mechanism for coping with adverse conditions in P. tobira.
Computational behavior of trihybrid Casson nanofluid blood flow occurring inside the conical gap between the rotating disk and the cone
Authors: Mehmood Yasir , Abbasi Waseem , Alhussein Musaed , Aurangzeb Khursheed , Rehman Asia , Anwar Muhammad Shahid 
Published: February 2024

Abstract
The investigation of the behavior of a trihybrid nanofluid flow inner the conoidal gap created among a revolving disc and a stationary cone is examined computationally in this study.The temperature and flow properties of the nanofluid, which is made up ofthree distinct kinds of nanoparticles, $Al_{2}O_{3}$, $TiO_{2}$ and Ag areexamined with the blood as base fluid. In the current work, heat transferthrough trihybrid nanoliquids that are passing through a gap between a discand a cone is also covered. In this case, the disc and cone may both berotating at the same rate or at different rates, or one may be stationary inrelation to the other. The heat transport and fluid flow patterns inside theconical gap are investigated using numerical simulations. In it, weinvestigated how many parameters, including rotating speed, cone angle, andconcentration of nanoparticles, effect heat transfer performance. Theoutcomes shed light on the complex interactions between the geometric andnanofluid characteristics, offering useful knowledge for fluid dynamics andthermal management applications. This fluid model is also useful for thestudy of blood pressure, arthritis, brain therapy and malignant tumor. Thegraphs are plotted using the MATLAB programme BVP4C to ensure convergence.Several variables, such as a magnetic parameter, Prandtl number, and Reynoldsnumbers, have an impact on temperature and velocity profiles.
Effect of SGLT2 Inhibitor Dapagliflozin on Biomarkers of Acute Kidney Injury in Patients with Acute Heart Failure: A Randomized Controlled Trial
Authors: Wattanakul Jananya , Gojaseni Pongsathorn , Chuasuwan Anan , Chittinandana Anutra 
Published: February 2024

Abstract
Sodium-glucose co-transporter 2 (SGLT2) inhibitors improve renal and cardiovascular outcomes in heart failure patients, and there is growing evidence that it would decrease the risk of acute kidney injury (AKI). The aim of this study was to assess the effect of SGLT2 inhibitor on biomarkers of AKI in patients with acute heart failure (AHF). Patients who hospitalized for AHF were randomized to dapagliflozin added to standard of care or control group for 28 days. The primary outcome was the change of urinary [TIMP-2] x [IGFBP7] by NephroCheck® from baseline. A total of 25 patients were enrolled, with 12 receiving dapagliflozin and 13 serving as controls. Compared with control group, dapagliflozin group significantly reduced urinary [TIMP-2] x [IGFBP7] after 7 days [dapagliflozin: -0.03 ± 0.11 (ng/mL)2/1000; control: +0.4 ± 0.14 (ng/mL)2/1000; P = 0.022] and continue this trend until the end of the study. In terms of clinical outcomes, dapagliflozin has demonstrated a trend towards decrease in AKI events compared to the control group (33.3% vs 46.2%; P = 0.513). The changes in serum creatinine, and adverse events showed no differences in either group. In conclusion, initiation of SGLT2 inhibitors in patients with AHF significantly decrease the urinary AKI risk markers TIMP-2 and IGFBP7, that supported protective effect of SGLT2 inhibitor on renal tubular injury.
A dynamically gated triangular DNA nanopore for molecular sensing and cross-membrane transport
Authors: Liu Xiaoming , Liu Fengyu , Chhabra Hemani , Maffeo Christopher , Huang Qiang , Aksimentiev Aleksei , Arai Tatsuo 
Published: February 2024

Abstract

Synthetic membrane nanopores made of DNA are promising systems to sense and control molecular transport in biosensing, sequencing, and synthetic cells. Dynamically gating cargo transport like the natural ion channels and systematically increasing the lumen size have become long-standing desires in developing nanopores. Here, we design a triangular DNA nanopore with a large dynamically-gated lumen. It can switch between expanded and contracted states without changing its stable triangular shape, whereby specific DNA bindings as stimuli mechanically pinch and release the three corners of the triangular frame. Transmission electron microscopy images and molecular dynamics simulations illustrated the large lumen up to 539 nm2, the stable architectures, and the high shape retention. Single-channel current recordings and fluorescence influx studies demonstrated the low-noise repeatable readouts and the controllable cross-membrane macromolecular transport. We envision that the proposed DNA nanopores could offer powerful tools in molecular sensing, drug delivery, and the creation of synthetic cells.
Bacteriocin-induced mechanism of Wickerhamomyces anomalus Y-5 co-cultured with Lactiplantibacillus paraplantarum RX-8 by transcriptomic and proteomic analysis
Authors: Liu Yangshuo , Nie Rong , Sun Haoxuan , Du Jing , Liu Guorong 
Published: February 2024

Abstract
Bacteriocin is a broad-spectrum antimicrobial active metabolite with a high potential for application in the food field. The previous studies found that co-culture of Wickerhamomyces anomalus Y-5 and Lactiplantibacillus paraplantarum RX-8 could increase the production of plantaricin RX-8. In order to investigate the induced mechanism of W. anomalus Y-5 in co-culture, this study explored the effects of induction components and contact mode on plantaricin RX-8; followed by transcriptomic and proteomic analyses of W. anomalus Y-5 in mono and co-culture systems, and screened differential metabolites by targeted metabolomic; finally, the potential inducing substances were subjected to validation experiments. The results indicated that the induced effect may not require direct cell contact, rather secretions constant stimulation. In co-culture system, W. anomalus Y-5 reduced nitrogen uptake, which allowed the release of the active Tap42 protein into the cytoplasm to stimulate the expression of retrograde genes, maintained biosynthesis of glutamic acid and glutamine. In addition, W. anomalus Y-5 was subjected to acid and osmotic stress, which resulted in activation of cAMP synthesis, inhibition of Ras protein activity, and up-regulation of Hxk2 expression. Further, we found that glutamine, inosine, guanosine, adenine, uracil, fumaric acid and pyruvic acid were the key substances that induced the production of plantaricin RX-8, and the optimal addition time was 8 h. In conclusion, these findings provided new perspectives on the identification of inducing substances and the analysis of production pathways in the efficient synthesis of bacteriocin induced by fungi, and lay the foundation for the industrial production of bacteriocin.
The construction of pH-sensitive starch-based carrier to control the delivery of curcumin for preservation
Authors: Guo Xiaojia , Liu Shujin , Yang Lanyan , Zhao Chaoguang , Shi Liu , Xiong Guangquan , Chen Lang , Chen Sheng , Wu Wenjin , Wang Lan 
Published: February 2024

Abstract
The controlled release of curcumin by the pH-sensitive carrier can achieve a long-term preservation effect, which is beneficial to extend the shelf-life of fish. The FTIR, XRD, 1H NMR, zeta potential, swelling ratio, and TG indicates the pH-sensitive starch-based carrier with a narrow pH-sensitive range (pH 6-7) is successfully fabricated by the carboxymethyl starch grafted with methacrylic acid first and then cross-linked with β-cyclodextrin to achieve dual-functionalities. The FTIR, XRD, and fluorescence spectroscopy reveals the mechanism of curcumin encapsulated by starch-based carrier is related to hydrophobic interactions and inter-molecular hydrogen bonding. The encapsulated curcumin shows improved stability, enjoyable antioxidant activity, antibacterial activity, and biocompatibility. The pH, TVB-N, TVC, and drip loss tests prove the application of carrier-curcumin complex with the concentration of 10 mg/mL on the preservation of yellow catfish can extend the shelf-life for 2-4 days with chilling storage and improve the storage quality of fillets. This work provides a dual-functionalities strategy to construct a pH-sensitive starch-based carrier to deliver curcumin and offers a promising choice in fish preservation.
Is Engagement in Social Activities with Peers within Senior Centers Associated with Participation in Health Check-ups?
Authors: ko ah jung , Kim Jinhyun , Park Eun-Cheol , Ha Min Jin 
Published: February 2024

Abstract
The global older adult population is increasing. Early detection and intervention through screenings are crucial for successful aging, as they play a significant role in identifying and addressing diseases. This study explored the relationship between the utilization of senior centers and the promotion of health check-ups. It utilized data from 10,097 individuals aged 65 years and above, sourced from the 2020 Elderly Survey. The primary variable of interest was the utilization of senior centers, which was classified into two groups. Subgroups were further categorized based on the frequency of usage and the presence of family members among facility users. Logistic regression analyses were conducted to assess the association between the utilization of senior centers and participation in health screenings. Both men and women utilizing older adult facilities demonstrated a higher likelihood of participating in health screenings compared with those who did not use these facilities. Participants visiting older adult facilities in a week exhibited a progressively higher likelihood of engaging in health screenings compared with those who visited such facilities zero times a week. Senior centers can serve as effective intervention methods to enhance health check-ups among older adults. Furthermore, this can contribute to fostering successful aging among older adults.
Effects of OPN and LIF expression in decidual and villous tissues on unexplained recurrent spontaneous abortion in the first trimester of pregnancy
Authors: Wen Xinling , Chen Binli , Yu Xuewen , Wang Li 
Published: February 2024

Abstract
Objective
Recurrent spontaneous abortion (RSA) is defined as a condition in which women suffer from two or more continuous spontaneous miscarriages. However, the etiology of a large proportion of women with RSA remains unknown. The study was conducted to investigate the effects of expression of osteopontin (OPN) and leukemic inhibitory factor (LIF) expression in decidual and villous tissues on unexplained RSA in the first trimester of pregnancy.
Methods
Twenty women with unexplained RSA in the first trimester of pregnancy were selected as study group, as well as 20 women with normal induced abortion in the first trimester of pregnancy were selected as control group. The decidual and villous tissues of participants in the two groups were collected. The expressions of OPN and LIF were detected by immunohistochemistry (IHC), immunofluorescence, western blotting and real-time polymerase chain reaction. The correlation between the expressions of OPN and LIF was analyzed.
Results
The OPN and LIF were mainly expressed in uterine epithelial cells and decidual cells, as well as pregnancy trophoblastic cells in villous tissues. The positive rates of OPN and LIF expression in decidual and villous tissues of the study group were significantly decreased, and the IHC scores were significantly lower compared with control group (P < 0.05). In the decidual tissues, the OPN and LIF were mainly co-located in uterine epithelial cells and decidual cells. While in the villous tissues, OPN and LIF were mainly co-located in gestational trophoblastocytes. The fluorescence intensities of OPN and LIF in decidual and villous tissues of the study group were significantly lower than that of the control group (P < 0.05). In addition, the protein and mRNA expression levels of OPN and LIF in decidual and villous tissues in study group were also significantly decreased compared with control group (P < 0.05). The protein and mRNA expression levels of OPN and LIF in decidual and villous tissues of the two groups were significantly positively correlated (P < 0.01).
Conclusion
The expressions of OPN and LIF in decidual and villous tissues of patients with unexplained RSA in the first trimester of pregnancy were significantly decreased, which may lead to reduced endometrial receptivity, and then cause RSA.
Challenges experienced by nurse educators in promoting acquisition of clinical reasoning skills by the undergraduate nursing students: A qualitative exploratory study
Authors: Mwale Omero .G. , mukwato Patricia .k. , Makukula Marjorie .K. 
Published: February 2024

Abstract

Background: Acquisition of clinical reasoning skills has been shown to improve the quality of care provided to patients when nursing students are competent. However, teaching and learning clinical reasoning skills is difficult because of its complexity. The aim of this study was to explore the challenges experienced by nurse educators in promoting acquisition of clinical reasoning skills by undergraduate nursing students.
Methods: This study employed a qualitative exploratory research design underpinned by a social constructivism paradigm. The participants were the nurse educators from the two universities in Malawi. Twelve nurse educators were purposively sampled and recruited into the current study. Data were collected using semi structured interview guide. Twelve individual in-depth interviews were conducted. Data collection and initial data analysis occurred simultaneously following thematic analysis. The moral precepts of beneficence, justice, and regard for human dignity were upheld.
Results: The two major themes that emerged from the transcribed data as challenges experienced by nurse educators in promoting acquisition of clinical reasoning skills were: nursing student lack of interest in learning, lack of resources in the clinical area, knowledge gap among nurse educators, lack of qualified nurse educators and role modelling.
Conclusion: Nurse educators' challenges in promoting clinical reasoning skills can be addressed by fostering receptiveness to these challenges, thereby enhancing students' competences and improving patient care. Based on the findings of this study it is recommended that nurse educators should update their teaching skills if they are to guide the nursing students effectively. The clinical arena should have enough resources.
Effectiveness of room-of-error interventions for healthcare providers: A systematic review
Authors: Jung Su Jin , Kang Jiwon , Lee Youngjin 
Published: February 2024

Abstract

Background
Patient safety incidents are recognized as significant contributors to patient mortality, thus demanding immediate attention and strategic interventions in healthcare systems. The room-of-error education program serves as a solution, as it provides a case-based learning platform allowing nursing students to identify and resolve medical errors within a controlled environment systematically. This study aimed to identify the context, mechanisms, and outcomes of room-of-error training programs.
Methods
This study adopted a systematic review methodology aligning with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines. Comprehensive searches were conducted across key databases, including OvidMEDLINE, Embase, Cochrane, and CINAHL, by utilizing specific terms related to healthcare providers, nursing students, room-of-error education, medical errors, simulation training, and virtual intervention. Studies focusing on healthcare providers or students, training for error recognition, and room-of-error-related concepts were included. Risk of bias in the selected studies was assessed using the Risk Of Bias In Non-randomized Studies version 2.0 tool.
Results
The search strategy yielded 2,447 articles, with eight studies meeting the inclusion criteria. Predominantly quasi-experimental in design, these eight studies primarily focused on nurses as the target population. Simulations were found to be widely integrated into room-of-error programs, emphasizing skill performance and critical thinking. Diverse content, including topics such as medication errors and infection control, was found to be delivered through offline or virtual formats and group-based or individual education.
Conclusions
The findings provide valuable insights into the characteristics and outcomes of room-of-error training programs for healthcare professionals and students. This study emphasizes the significance of practical, case-based approaches in nursing education to augment knowledge, confidence, and competencies, thereby enhancing patient safety in clinical practice.
Voronoi Structure of Space Integration with Applied Roundabout Network
Authors: El-Shazly Ali 
Published: February 2024

Abstract
Network objective of structuring space integration addresses topologic and geometric dimensions to be framed-out in holistic method. In this sense, the spatial network of multidiscipline voronoi convex-hull encapsulates the varied integral properties with their correlative exploration. In practice, conservational network of roundabout Cairo investigates its integral structure against decomposing. Resulted structural essence defines individual topologic versus plural geometric role at global integration per generator. The integrated topology cross-correlates control syntax with degree centrality at the local context, whereas the global structure of asymmetry complements betweenness dynamics of generators’ distributiveness. Meanwhile, geometrical network of gabriel-graph forms cyclic spaces of global integrity and correlated by voronoi nodes at equidistant meeting-point per cycle. Overlaid voronoi properties classify the layout into clustered generators of correlated interval integrity with functional cognition to infinity. Overview of voronoi network adopts the intangible space integration along its comprehensive framework of interrelated algorithms in a virtual reality of urbanism.
Acellular spinal cord scaffold containing quercetin-encapsulated nanoparticles plays an anti-inflammatory role in functional recovery from spinal cord injury in rats
Authors: Ebrahimi Babak , Mokhtari Tahmineh , Ghaffari Neda , Adabi Mahdi , Hassanzadeh Gholamreza 
Published: February 2024

Abstract

Inflammatory responses play a crucial role in the pathophysiology of spinal cord injury (SCI) and developing new approaches to establish an anti-inflammatory environment for the promotion of neuroregeneration holds promise as a potential approach. In this study, our aim was to investigate the potential of combining an acellular spinal cord scaffold (ASCS) with quercetin-loaded bovine serum albumin (Qu/BSA) nanoparticles (NPs) for the treatment of SCI. The ASCS was prepared using physical and chemical methods, while the Qu/BSA NPs were prepared through a desolvation technique. The NPs exhibited favorable characteristics, including a mean size of 203 nm, a zeta potential of − 38, and an encapsulation efficiency of 96%. Microscopic evaluation confirmed the successful distribution of NPs on the walls of ASCS. Animal studies revealed that Qu/BSA NPs effectively regulated the gene expression and protein levels of NLRP3, ASC, and Casp1. Moreover, treatment with ASCS containing either blank BSA (B/BSA) NPs or Qu/BSA NPs effectively promoted functional recovery via increasing the amount of nestin- and glial fibrillary acidic protein (GFAP)-positive cells in the site of injury. Notably, Qu/BSA/ASCS exhibited superior outcomes compared to B/BSA/ASCS. Overall, the combination of ASCS with the Qu delivery system presents a promising therapeutic approach for SCI by inhibiting inflammatory responses and promoting neuroregeneration, leading to the restoration of motor function in animals. This study demonstrates the potential of utilizing biomaterials and NPs to enhance the effectiveness of SCI treatment.