bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the in vitro probiotic properties of Ligilactobacillus salivariusJCF5 and its impact on Jersey yogurt quality
Authors: Lv Yi , Xu Chunyue , Sun Qingshen 
Published: January 2025

AbstractBackgroundJersey milk, known for its high protein content, is an excellent base for yogurt production. Given that Jersey milk is derived from Jersey cows, this study was to isolate probiotics from Jersey cow feces and investigate their potential as alternative starter cultures for fermenting Jersey milk. Our goal was to develop new starter cultures specifically suited for Jersey yogurt production, while also contributing to the diversity of fermentation agents available for dairy products. This study aimed to evaluate the probiotic functions of Ligilactobacillus salivarius isolated from the feces of newborn Jersey calves after colostrum consumption and to investigate its impact as a starter culture on the quality of Jersey yogurt.ResultsA lactic acid bacterial strain was screened through acid and bile salt tolerance tests and simulated gastrointestinal experiments. The strain survived up to 42.8% after 3 h of cultivation at pH 2, and its viable count after 3 h of cultivation in a medium containing 0.3% bile salt was 3 log(CFU mL−1). Additionally, the survival rates after 3 h of treatment with gastric and intestinal juices were 90.67 ± 0.41% and 84.97 ± 1.40%, respectively, indicating good acid and bile salt tolerance. Identification using 16S rDNA showed that the strain was L. salivarius JCF5. This strain improved the texture properties such as viscosity, elasticity and cohesiveness of yogurt when used in combination with commercial starter cultures.ConclusionLigilactobacillus salivarius JCF5 is a promising probiotic strain for enhancing the quality of Jersey yogurt. © 2025 Society of Chemical Industry.
GelMA Hydrogels Integrated With aptamer CH6‐Functionalized Tetrahedral DNA Nanostructures for Osteoporotic Mandibular Regeneration
Authors: Hong Shebin , Cui Ya , He Dongming , Wu Hao , Jiang Weidong , Cao Jian , Wang Xudong 
Published: January 2025

AbstractOsteoporotic bone regeneration is challenging due to impaired bone formation. Tetrahedral DNA nanostructures (TDN), promising nucleic acid nanomaterials, have garnered attention for their potential in osteoporotic mandibular regeneration owing to their ability to enhance cellular activity and promote osteogenic differentiation. Osteoblasts play a critical role in bone regeneration; however, intracellular delivery of TDN into osteoblasts remains difficult. In this study, a novel osteoblast‐targeted CH6 aptamer‐functionalized TDN (TDN‐CH6) is aimed to develop for osteoporotic mandibular regeneration. This results demonstrated that TDN‐CH6 exhibits superior osteoblast specificity and efficient recruitment to bone fracture sites. Furthermore, TDN‐CH6 significantly enhances cellular activity and osteogenic differentiation compared to TDN alone. Notably, Gelatin Methacryloyl (GelMA) hydrogels incorporating TDN and TDN‐CH6 shows improved biological performance and are favorable for osteoporotic mandibular regeneration, suggesting that this platform represents a promising strategy for addressing complex bone defects.
Color Inside and Outside the Lines: Evidence From Eye‐Tracking Studies on Conformity to and Differentiation From Category Color Codes
Authors: Cardamone Ernesto , Miceli Gaetano “Nino” , Raimondo Maria Antonietta 
Published: January 2025

ABSTRACTCategory color codes are commonly used hues in packaging within a specific product category. These colors establish visual standards for new products and influence consumer perception and product categorization. Product managers face a challenging decision: Should they conform to or differentiate from these established color norms? For example, should flour packaging adopt the typical white category color code or stand out with a distinctive color like purple? Retailers face a similar dilemma: Where should they place products that conform to or differ from these color norms to capture consumer attention effectively? Despite the relevance of category color codes for both manufacturers and retailers, the literature on this topic remains limited. Building upon the Limited Capacity Model of Motivated Mediated Message Processing, this paper examines how conformity or differentiation of main and secondary packaging colors from category codes impacts consumer attention. Findings from lab and field eye‐tracking studies reveal a negative interaction between the conformity of main and secondary colors with category codes on both the duration and number of fixations. Hybrid color combinations—where either the main or secondary color conforms while the other differentiates—are most effective in attracting consumer attention, which, in turn, influences consumer choice. This research expands the understanding of the trade‐off between conformity and differentiation in packaging color, offering theoretical contributions to the role of visual stimuli in attracting attention and providing practical guidance for managers in selecting optimal color combinations for their products.
Long‐Lasting Cross‐Linked PLGA‐Inspired Nanoparticles from One‐Pot Nanopolymerization of Precisely Sequenced Short Oligolactoglycolic Acid Dimethacrylates
Authors: Blagojevic Luka , Kamaly Nazila 
Published: January 2025

AbstractA novel PLGA‐inspired NP polymerization technique is presented, which allows the formation of NPs via the cross‐linking of precisely sequenced short oligolactoglycolic acid dimethacrylates (OLGADMAs). Following the synthesis of a range of OLGADMAs, a library of NPs via this rapid and surfactant‐free nanopolymerization method is successfully generated, which permits the simultaneous NP formation and encapsulation of drugs such as dexamethasone. The results indicate that NPs produced through this nanopolymerization technique with precisely controlled sequences exhibit heightened stability compared to conventionally sequenced and non‐sequence controlled PLGA, as evidenced by minimal pH changes over five weeks. This improved stability is attributed to simultaneous crosslinking and co‐polymerization of the OLGADMAs. Moreover, the long‐acting NPs demonstrate minimal cytotoxicity and uniform cellular uptake in vitro. It is concluded that the ability to precisely regulate the sequence of short PLGA‐inspired monomers and employ a unique in situ nanopolymerizing reaction results in exceptionally stable NPs for sustained drug delivery and opens exciting possibilities for the development of a range of long‐lasting drug delivery systems with programmable structure and function.
Aptamer‐Coupled Polymer‐Grafted Fe3O4 Nanoparticles for Highly Efficient Isolation of Exosomes
Authors: Huang Daqiang , Yu Junjun , Tian Jia , Cai Haibo , Zhang Weian 
Published: January 2025

AbstractExosomes, the bioactive particles secreted by various cells, are essential in mediating cellular communication. However, their small size and the interference from non‐exosome proteins present significant hurdles for their rapid and non‐destructive capture and release. To overcome these obstacles, a promising strategy to efficiently and selectively isolate exosomes from mesenchymal stem cells (MSCs) is developed by using CD63 aptamer‐conjugated magnetic nanoparticles (Fe3O4‐Aptamer). The Fe3O4 nanoparticles are first modified by RAFT polymerization of N‐(methacryloyloxy) succinimide and oligoethylene glycol methacrylate, and subsequently, CD63 aptamers are grafted onto the surface of nanoparticles to produce Fe3O4‐Aptamer. These aptamer units act as a “lock and key” recognition with the CD63 proteins on exosomes, enabling specific binding to exosomes. The Fe3O4‐Aptamer can efficiently capture exosomes in a conditioned medium, and be easily collected by an external magnetic field, facilitating the facile collection and multiple‐cycle reuse of Fe3O4‐Aptamer. By introducing the complementary sequence of the CD63 aptamer, the captured exosomes can be rapidly released from Fe3O4‐Aptamer because of the stronger binding affinity between the complementary sequence and the aptamers. When utilized for exosome isolation, the exosome‐capture and release efficiency of Fe3O4‐Aptamer can achieve up to ca. 82.9% and 96.1%, respectively. Thus, Fe3O4‐Aptamer offers a promising and facile strategy for the highly efficient isolation of exosomes.
Neutrophil heterogeneity and plasticity: unveiling the multifaceted roles in health and disease
Authors: He Weifeng , Yan Lingfeng , Hu Dongxue , Hao Jianlei , Liou Yih‐Cherng , Luo Gaoxing 
Published: January 2025

AbstractNeutrophils, the most abundant circulating leukocytes, have long been recognized as key players in innate immunity and inflammation. However, recent discoveries unveil their remarkable heterogeneity and plasticity, challenging the traditional view of neutrophils as a homogeneous population with a limited functional repertoire. Advances in single‐cell technologies and functional assays have revealed distinct neutrophil subsets with diverse phenotypes and functions and their ability to adapt to microenvironmental cues. This review provides a comprehensive overview of the multidimensional landscape of neutrophil heterogeneity, discussing the various axes along which diversity manifests, including maturation state, density, surface marker expression, and functional polarization. We highlight the molecular mechanisms underpinning neutrophil plasticity, focusing on the complex interplay of signaling pathways, transcriptional regulators, and epigenetic modifications that shape neutrophil responses. Furthermore, we explore the implications of neutrophil heterogeneity and plasticity in physiological processes and pathological conditions, including host defense, inflammation, tissue repair, and cancer. By integrating insights from cutting‐edge research, this review aims to provide a framework for understanding the multifaceted roles of neutrophils and their potential as therapeutic targets in a wide range of diseases.
Butyrolactone I blocks the transition of acute kidney injury to chronic kidney disease in mice by targeting JAK1
Authors: Zhang Zijun , Zhao Ziming , Qi Changxing , Zhang Xiaotian , Xiao Yang , Chen Chengjuan , Zou Yu , Chen Xia , Gu Lianghu , Huang Jianzheng , Huang Kun , Xiang Ming , Zhang Tiantai , Tong Qingyi , Zhang Yonghui 
Published: January 2025

AbstractChronic kidney disease (CKD) is a disease that affects more than 850 million people. Acute kidney injury (AKI) is a common cause of CKD, and blocking the AKI–CKD transition shows promising therapeutic potential. Herein, we found that butyrolactone I (BLI), a natural product, exerts significant nephroprotective effects, including maintenance of kidney function, inhibition of inflammatory response, and prevention of fibrosis, in both folic acid‐ and ureteral obstruction‐induced AKI–CKD transition mouse models. Notably, BLI showed greater blood urea nitrogen reduction and anti‐inflammatory effects than telmisartan. Bioinformatics analysis and target confirmation assays suggested that BLI directly binds to JAK1, and kinase inhibition assay confirmed it is a potent JAK1inhibitor with an IC50 of 0.376 µM. Experiments in JAK1‐knockdown mice also proved that BLI targets JAK1 to work. Furthermore, BLI demonstrated nephroprotective effects and safety comparable to ivarmacitinib, the well‐known JAK1 inhibitor. Mechanistically, BLI targets JAK1 and inhibits its phosphorylation and JAK‐STAT activation, subsequently regulating the downstream signaling pathways to inhibit reactive oxygen species production, inflammation, and ferroptosis, thereby preventing the occurrence of kidney fibrosis and blocking the AKI–CKD transition process. This study demonstrates for the first time that BLI is a JAK1 inhibitor and a promising candidate for delaying CKD progression, which warrants further investigation.
Risk of Motor Vehicle Accidents in Obstructive Sleep Apnea: Comparative Analysis of CPAP Versus Surgery
Authors: Sina Elliott M. , Shankar Somya , Boon Maurits S. , Huntley Colin T. 
Published: January 2025

AbstractObjectiveTo compare the incidence of motor vehicle accidents (MVAs) among patients with obstructive sleep apnea (OSA) undergoing continuous positive airway pressure (CPAP) therapy and sleep surgery.Study DesignRetrospective cohort study using the TriNetX national clinical database.SettingAnalysis of a nationwide patient cohort.MethodsThe database was queried for patients with OSA (International Classification of Diseases, 10th Revision: G47.33) treated with CPAP (Current Procedural Terminology [CPT]: 94660), sleep surgery (CPT: 42145, 64568, 64582), or no treatment. Sleep surgeries included uvulopalatopharyngoplasty and hypoglossal nerve stimulation. 1:1 Propensity score matching (PSM) ensured cohort comparability by age, sex, and race. MVA incidence rates were analyzed, along with a subanalysis of OSA‐related comorbidities as a surrogate of OSA severity.ResultsA total of 2,832,437 patients with OSA were identified. Patients with OSA undergoing sleep surgery demonstrated a significantly lower incidence of MVAs (3.403%) compared to the OSA + CPAP cohort (6.072%) and the no‐treatment group (4.662%). The odds ratio (OR) for MVA incidence in the no‐treatment group compared to the OSA + sleep surgery cohort was 1.214 (95% confidence interval [CI]: 1.060‐1.391, P = .0051). The OR in the OSA + sleep surgery cohort compared to the CPAP cohort was 0.545 (95% CI: 0.480‐0.618, P < .0001). Patients with OSA who experienced MVAs were more likely to have comorbidities such as hypertension, diabetes, and heart failure following the accident.ConclusionSleep surgery significantly reduces the risk of MVAs in patients with OSA compared to CPAP and no treatment. In appropriate candidates, surgery should be considered to mitigate personal and public health risks associated with OSA.
Comparison of Vegetable Waste Byproducts of Selected Cultivars of Foeniculum vulgare Mill. by an Integrated LC‐(HR)MS and 1H‐NMR‐Based Metabolomics Approach
Authors: Crescenzi Maria Assunta , Cerulli Antonietta , Masullo Milena , Montoro Paola , Piacente Sonia 
Published: January 2025

ABSTRACTIntroductionThe metabolome of plants is influenced by various factors, especially environmental, as the season in which they are grown. So, distinct varieties of the identical plant might show an increase or decrease in metabolites. The diversity of content of primary and secondary metabolites can also determine the variation in their biological properties. Due to the current occurrence of various fennel varieties, the crop can now be grown for the entire year.ObjectiveThis work used an integrated approach of LC/MS and NMR analysis to characterize the metabolome of fennel waste of different varieties by multivariate statistical analysis.MethodsThe extracts were investigated by NMR and LC/MS analysis to focus attention on the primary and secondary metabolites. Both LC‐HRMS and NMR data were analyzed by principal component analysis (PCA).ResultsThe 1H‐NMR analysis led to the identification of 15 primary metabolites, such as amino acids, carbohydrates, and organic acid derivatives. The secondary metabolites identified by LC/MS analysis mainly belong to the phenolic, lipid, and fatty acid compounds classes.ConclusionThis integrated approach guarantees a precise and complete overview of the variations in the metabolic expression of the fennel varieties grown in different seasons.
Electrospun chitosan/polycaprolactone wound dressing containing henna extract and vitamin C: A natural composite for wound care
Authors: Sadeghimanesh Maryam , Poorazizi Elahe 
Published: January 2025

AbstractThe present study shows an incredibly promising wound dressing material based on electrospun chitosan (CS)/polycaprolactone (PCL) fibers that are enhanced with henna extract (HE) and vitamin C (VitC). The incorporation of HE, a traditional medicinal herb known for its wound healing capabilities, and Vit C, a powerful antioxidant, into CS takes use of CS's natural biocompatibility and antibacterial characteristics. This combination requires to generate a synergistic effect that enhances wound healing and provides protection against infection. The scaffolds that were created were investigated to determine their surface morphology, chemical composition, ability of water swelling, strength, and ability to kill bacteria. The results indicate that the inclusion of HE and Vit C substantially improved the ability of the wound dressing to absorbing water, which is essential for encouraging the attachment of cells and maintaining moisture, both of which are critical for the healing of wounds. Furthermore, the wound dressing containing HE shown significant antibacterial efficacy against both Escherichia coli and Staphylococcus aureus, demonstrating its capacity to prevent infection. According to in vitro evaluation, the sample containing 1 wt% HE and VitC exhibited exceptional biocompatibility and cell adhesion with the L929 fibroblast cell line. This study introduces an innovative wound dressing that has a customized mixture of natural substances. This innovative approach addresses gaps in previous CS/PCL studies, introducing a promising natural composite for advanced wound care applications.Highlights
Electrospun CS/PCL‐HE/VitC wound dressing enhanced wound healing potential.
HE and VitC improve the biocompatibility and antimicrobial properties.
The scaffold demonstrates moisture retention for wound healing application.
HE enhances the antibacterial activity against E. coli and S. aureus.
In vitro studies show excellent cell adhesion with L929 fibroblast cells.
Bullying Victimization, Psychological Pain, and Suicidal Ideation: Testing the Buffering Hypothesis of Social Support Among Saudi School Students
Authors: El Keshky Mogeda El Sayed , Alganami Badra Hamdi 
Published: January 2025

ABSTRACTBullying is a worrisome problem for school‐age youths, particularly as it has been associated with suicidal ideation among students. The psychological pain theory of suicide and the buffering hypothesis of social support as a protective factor have been established, but have not been tested among Saudi students. The aim of this study was to investigate the association between bullying victimization and suicidal ideation, the mediating role of psychological pain, and the moderating role of social support among Saudi students. A sample of 585 school students (59.5% female, mean age = 16.19, SD = 1.87, range = 12–18) were involved in this study. Relationships in a mediation model and a moderated mediation model were tested using RStudio. Bullying victimization was positively related to suicidal ideation. The mediation model indicated that psychological pain mediated this relationship. The moderated mediation model showed that the indirect relationship of bullying with suicidal ideation through psychological pain was stronger in those students with little support from family, friends, and significant other. This study highlighted the importance of targeting psychological pain and interpersonal relationships in suicide interventions and supported the buffering hypothesis of social support as a protective mechanism.
Arabidopsis 3‐Deoxy‐d‐Arabino‐Heptulosonate 7‐Phosphate (DAHP) Synthases of the Shikimate Pathway Display Both Manganese‐ and Cobalt‐Dependent Activities
Authors: Yokoyama Ryo , Maeda Hiroshi A. 
Published: January 2025

ABSTRACTThe plant shikimate pathway directs a significant portion of photosynthetically assimilated carbon into the downstream biosynthetic pathways of aromatic amino acids (AAA) and aromatic natural products. 3‐Deoxy‐d‐arabino‐heptulosonate 7‐phosphate (DAHP) synthase (hereafter DHS) catalyzes the first step of the shikimate pathway, playing a critical role in controlling the carbon flux from central carbon metabolism into the AAA biosynthesis. Previous biochemical studies suggested the presence of manganese‐ and cobalt‐dependent DHS enzymes (DHS‐Mn and DHS‐Co, respectively) in various plant species. Unlike well‐studied DHS‐Mn, however, the identity of DHS‐Co is still unknown. Here, we show that all three DHS isoforms of Arabidopsis thaliana exhibit both DHS‐Mn and DHS‐Co activities in vitro. A phylogenetic analysis of various DHS orthologs and related sequences showed that Arabidopsis 3‐deoxy‐D‐manno‐octulosonate‐8‐phosphate synthase (KDOPS) proteins were closely related to microbial Type I DHSs. Despite their sequence similarity, these Arabidopsis KDOPS proteins showed no DHS activity. Meanwhile, optimization of the DHS assay conditions led to the successful detection of DHS‐Co activity from Arabidopsis DHS recombinant proteins. Compared with DHS‐Mn, DHS‐Co activity displayed the same redox dependency but distinct optimal pH and cofactor sensitivity. Our work provides biochemical evidence that the DHS isoforms of Arabidopsis possess DHS‐Co activity.
Resmetirom and Thyroid Hormone Receptor‐Targeted Treatment for Metabolic Dysfunction‐Associated Steatotic Liver Disease (MASLD)
Authors: Liu Chang‐Hai , Zeng Qing‐Min , Hu Teng‐Yue , Huang Yu , Song Yongfeng , Guan Haixia , Rockey Don C. , Tang Hong , Li Sheyu 
Published: January 2025

AbstractMetabolic dysfunction‐associated steatotic liver disease (MASLD) is a rapidly increasing chronic disease worldwide, particularly among patients with type 2 diabetes. Its severe form, metabolic dysfunction‐associated steatohepatitis (MASH), is also on the rise. The treatment of MASLD and MASH poses significant challenges. Thyroid hormones and their receptors thyroid hormone receptor (TR) agonists, especially resmetirom, have shown potential in improving metabolism and reducing liver inflammation. Thyroid hormones play a crucial role in regulating metabolism and maintaining physiological balance. However, in patients with MASLD, there is a reduced conversion of 3,3′,5,5′‐tetraiodo‐l‐thyronine (T4) to biologically active 3,5,3′‐triiodo‐l‐thyronine (T3), resulting in decreased T3 levels and impaired hepatic TR signaling. This hormonal imbalance is associated with disrupted hepatic lipid metabolism. Resmetirom, an oral selective TR agonist that specifically targets hepatocytes, was approved by the Food and Drug Administration (FDA) in March 2024 for the treatment of moderate to severe liver fibrosis in non‐cirrhotic adults with MASH. This approval was based on the results of the MAESTRO clinical program, which includes multiple‐stage research designs such as the MAESTRO‐NASH, MAESTRO‐NAFLD‐1, MAESTRO‐NAFLD‐OLE, and MAESTRO‐NASH‐OUTCOMES, aims to evaluate the efficacy and safety of resmetirom in different populations of MASH patient. Although the approval of resmetirom represents a significant milestone in the treatment of MAFLD and MASH, many questions remain regarding its long‐term effectiveness and impact on clinical outcomes. Ongoing research, particularly through the MAESTRO program, holds promise for providing additional insights into the long‐term management of MASLD using resmetirom and other similar medications.