bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Four Hereditary Protein C Deficiencies Associated with Vascular Thromboembolism
Authors: Chen Xuanyu , Yuan Chengxiang , Hu Beilei , Lu Chunxing , Wang Yujia , Han Zhao , Zou Ming 
Published: December 2023

Abstract
Objective
To analyze the clinical features and gene mutations in four families with hereditary protein C (PC) deficiency and explore their association with vascular thromboembolism.
Methods
The clinical data of four patients with PC deficiency were retrospectively analyzed. Venous blood samples were collected from the four affected patients and their family members, and relevant coagulation indexes and thrombin production and inhibition tests were performed. PCR was used to amplify and directly sequence the PROC gene of the prevalent patients. Software analysis was conducted to assess the conservativeness and pathogenicity of the mutated loci. Protein models were constructed to analyze the spatial structure before and after the mutation.
Results
Thrombin generation and inhibition assays demonstrated impaired anticoagulation in all four probands. Proband 1 and 4 presented clinically with pulmonary embolism and lower extremity deep vein thrombosis (DVT), Proband 2 with cerebral infarction, and Proband 3 with DVT. Genetic analysis revealed the presence of the following mutations: c541T > G heterozygous missense mutation, c.577-579delAAG heterozygous deletion mutation, c.247-248insCT heterozygous insertion mutation, c.659G > A heterozygous missense mutation, and a new variant locus c.1146_1146delT heterozygous deletion mutation in the four probands, respectively. Conservativity and pathogenicity analyses confirmed that most of these amino acid residues were conserved, and all the mutations were found to be pathogenic. Analysis of protein modeling revealed that these mutations induced structural alterations in the protein or led to the formation of truncated proteins.
Conclusion
The identified mutations are likely associated with decreased PC levels in each of the four families. The clinical manifestations of hereditary PC deficiency exhibit considerable diversity.
Balancing multiple speeds flexible rotors without trial weights using multi-objective optimization
Authors: Yang Fengyu , Yao Jianfei , Jiao Shiwen , Scarpa Fabrizio , Li Yan 
Published: December 2023

Abstract
This paper proposes a multi-objective optimization method for the optimal balancing of flexible rotors without using trial weights. Finite elements are first used to develop a dynamic model of the flexible rotor. An optimization method and an inverse identification approach are then used to minimize the objective function by considering constraints and vibration levels of the flexible rotor during balancing. Finally, an optimal balancing scheme is solved by using a genetic algorithm. The optimization process here described consists in a global multi-objective optimization framework transformed into single-objective (SO) and dual-objective (DO) optimizations. Simulations and experiments are carried out on a three-discs flexible rotor balancing rig without trial weights, to benchmark the multi-objective optimization technique proposed here. The results show that the method is efficient to avoiding multiple starts and stops during the balancing process, and also provides an enhanced balancing effect.
Cytomegalovirus infection during daratumumab therapy in patients with newly diagnosed multiple myeloma
Authors: Kikuchi Taku , Tsukada Nobuhiro , Kunisada Kodai , Nomura-Yogo Moe , Oda Yuki , Sato Kota , Takei Tomomi , Ogura Mizuki , Abe Yu , Suzuki Kenshi , Ishida Tadao 
Published: December 2023

Abstract

The introduction of daratumumab has improved the treatment outcomes for multiple myeloma (MM). However, infectious complications such as pneumonia are a concern in patients receiving daratumumab. Although some reports have explored the association between daratumumab and cytomegalovirus (CMV) infection, most of these have focused on relapsed or refractory cases. There are few reports on patients with newly diagnosed MM (NDMM). In this study, we retrospectively analyzed CMV infections in 53 patients with NDMM who received daratumumab as induction therapy. CMV infection was defined as CMV antigenemia positivity. Patients who did not undergo CMV antigenemia testing were considered to have no CMV infection. The frequency of CMV infection was calculated as the cumulative incidence rate, considering death during daratumumab administration without CMV infection as a competing event. The median age at treatment initiation was 71 years (range, 50–82 years), and 50.9% of the patients were female. The median duration of daratumumab administration was 10.0 months (range, 0.3–63.8 months). Nine patients developed CMV infection, and the cumulative incidence rate at six months was 18.1% (95% confidence interval: 8.9–30.1%). One patient experienced CMV retinitis and required antiviral therapy, while the remaining eight patients did not require treatment and could be managed through observation. During daratumumab treatment for NDMM, very few cases of CMV infection required treatment. However, the incidence of CMV infection was relatively high, suggesting that regular monitoring of CMV is worth considering for more appropriate management with daratumumab treatment.
Gender difference: Men less frequently but more severely affected in Fuchs endothelial corneal dystrophy (FECD)
Authors: Zwingelberg Sarah Barbara , Dirisamer Martin , Mayer Wolfgang J , Priglinger Siegfried , Luft Nikolaus 
Published: December 2023

Abstract
Purpose: Comparison of pre-operative findings and outcome of Descemet's membrane endothelial keratoplasty (DMEK) in female versus male patients with Fuchs endothelial corneal dystrophy (FECD).Methods: Records of consecutive DMEK surgeries performed between 2015 and 2019 at the Department of Ophthalmology at the University of Cologne and LMU Munich were retrospectively reviewed. Eyes with FECD with a complete 12 months follow-up were enrolled. Main outcome parameters included central corneal thickness (CCT), peripheral corneal thickness (PCT), best spectacle corrected visual acuity (BSCVA, log MAR), and endothelial cell count (ECC) before and after DMEK.Results: 144 eyes from 144 patients could be included in the study with FECD (female (f) n = 95, male (m) n = 49, ~ f:m:2:1). Preoperatively, CCT (f: 678.3 ± 128.8µm; m: 747.0 ± 148.0µm) and PCT (f: 702.6 ± 65.1µm; m: 782.4 ± 105.1µm) were both significantly higher in the male group (p < 0.01). Twelve months after DMEK, CCT (w: 541.0 ± 47.0µm; m 540.7 ± 37.5µm) showed no significant difference between the groups (p = 0.980), whereas the PCT showed a significant difference (f: 681.5 ± 66.3µm; m: 722.6 ± 71.7µm), p = 0.011. The increase in PCT correlated with accelerated ECC loss 12 months after DMEK (p = 0.009). Prior to DMEK, BSCVA in female FECD patients was higher, but without a significant difference (w: 0.38 ± 0.22logMAR; m 0.45 ± 0.34LogMAR, p = 0.256) compared to male patients with FECD. After 12 months, BSCVA improved in both groups without significant difference (p = 0.245).Conclusions: A large number of studies, including our present one, have consistently shown that women are more frequently affected by FECD. Our study suggests for the first time that men may be less frequently, but nevertheless more severely affected by FECD in terms of ECC loss and a higher CCT and PCT. Therefore, further gender-specific analyses should be performed in the disease of FECD.
Exploring the Causal Relationship between Gut Bacteria and DLBCL through Comprehensive Integration of Prior Studies
Authors: Chen Haoqing , Gao Yan , Chen Tingting , He Yanxia , Ping Liqin , Huang Cheng , Huang Huiqiang 
Published: December 2023

Abstract
Previous research has revealed a significant association between the gut microbiome and diffuse large B-cell lymphoma (DLBCL). However, the findings of these studies have yet to be entirely consistent. Whether a causal relationship exists between gut bacterial and DLBCL remains elucidated. We performed two-sample mendelian randomization (MR) using genetic data from MiBioGen and DLBCL summary statistics from GWAS. The primary analysis used inverse variance weighted (IVW), the weighted median, MR-Egger regression, and pleiotropic residual sum and outlier tests. Reverse MR checked for reverse causality. Our study identified four bacterial genera can causally increase the risk of DLBCL disease: Ruminococcus torques.id.14377 (OR 0.44; p = 0.006), Ruminococcaceae UCG014.id.11371 (OR 1.69; p = 0.028), Ruminococcaceae UCG002.id.11360 (OR 0.62; p = 0.023), and Eubacterium oxidoreducens.id.11339 (OR 1.80; p = 0.033). In reverse MR analysis, we found no causal effect from DLBCL to gut bacterial. Our investigation offers indications of causal connections between the gut microbiome and the onset of DLBCL.
Neuropathogenic Equid Herpesvirus 1 involving in horse respiratory disease outbreak, north Xinjiang, China, 2021-2023
Authors: Tong Panpan , Yang Enhui , Liu Bin , Pan JuanJuan , Dang Yueyi , Tian Shuyao , Palidan Nuerlan , Jia Chenyang , Kuang Ling , Wang Chuanfeng , Lu Gang , Xie Jinxin 
Published: December 2023

Abstract
Background: Equid herpesvirus 1 (EHV-1) is one of important viral pathogens that frequently cause respiratory disease in horse cross the worldwide. Until now, no information on EHV-1 presents in Chinese horse respiratory disease. An acute respiratory disease outbreak in horse population of north Xinjiang between April 2021 and May 2023.
Results: qPCR detection revealed the presence of EHV-1 in all samples as the possible cause of all
respiratory disease, although EHV-4, EHV-2 and EHV-5 were also found to circulate in a small portion
of samples. Three EHV-1 strains, caused respiratory disease in horses, were successfully isolated. Furthermore, the full ORF33 sequences comparison and phylogenetic analysis indicated that indicated that the isolates could have been origin from EHV-1 trains detected in Yili horse abortions. In addition, based on the predicted ORF30 nucleotide sequence, it was found that all the strains carried a guanine at the 2254 nucleotide position (aspartic acid at position 752 of the viral DNA polymerase) and were, therefore, identified as neuropathogenic strains.
Conclusion: This study is the first one that establishes EHV-1 as the cause of respiratory disease in horses, of China. Further characterization of the ORF30 sequences revealed that all the EHV-1 strains from the study carried the neuropathogenic genotype. Totally, neuropathogenic EHV-1 infection in China’s horse population should be concerned because the virus detected in both respiratory disease and abortions.
Newly isolated Limosilactobacillus reuteri B1/1 modulates cell response in porcine ex vivo model mirroring the gut
Authors: Kiššová Zuzana , Štofilová Jana , Mudroňová Dagmar , Karaffová Viera 
Published: December 2023

Abstract
The epithelia of the intestine perform various functions, playing a crucial role in providing a physical barrier and an innate immune defence against infections. Creating a 3D model of cell co-cultures established with IPEC-J2 cell line and porcine blood monocyte-derived macrophages (MDMs), we are getting closer to mirroring the porcine intestine ex vivo. The effect of Limosilactobacillus reuteri B1/1 and Limosilactobacillus fermentum CCM 7158 on relative gene expression of interleukins (IL-1β, IL-6, IL-8, IL-18 and IL-10), genes encoding receptors for TLR4 and TLR2, tight junction proteins as claudin-1 (CLDN1), occludin (OCLN) and important antimicrobial proteins as lumican (LUM) and olfactomedin-4 (OLMF-4) was monitored in this model. The immunomodulatory potential of newly isolated L. reuteri B1/1 was confirmed as was able to suppress the enhanced pro-inflammatory response to LPS induction in both cell types. L. reuteriB1/1 was even able to up-regulate the mRNA levels of genes encoding antimicrobial proteins LUM and OLFM-4 and to increase TJ-related genes CLDN1 and OCLN, which were significantly down-regulated by LPS-infection in IPEC-J2 cells. Conversely, L. fermentum CCM 7158 which was chosen as an indicator lactic acid bacteria (LAB) strain, increased the mRNA levels of the investigated pro-inflammatory cytokines (IL-18, IL-6, and IL-1β) in MDM, when LPS was simultaneously applied to basally deposited macrophages. Although L. fermentum CCM 7158 induced the production of pro-inflammatory cytokines, synchronous up-regulation of the anti-inflammatory cytokine IL-10 was detected in both lactic acid bacteria LAB strains used in both cell cultures.
Attitude and perception of Congolese and LGBT on homosexuality, and relationship between homosexuality and the conception of the profession
Authors: Kamuanga Michael Kapitene , Lubaki Jean-Pierre Fina , Nguala Philippe Lukanu , Kiswaya Ernest Sumaili 
Published: December 2023

Abstract

Context Worldwide, 72 countries criminalize homosexuality. Only one country in Africa recognizes homosexuality. While 10 countries criminalize it, against only one that protects it. Exceptionally, the DRC does not have legislation against or for homosexuality. The aim of this study was to describe the attitudes and perceptions of Congolese and sexual minorities on homosexuality.Methods This study was cross-sectional and analytical, conducted from February the 1St, 2021 to March the 30th, 2022, in the homosexual, bisexual and transgender population of Kinshasa town. The selection was made in homosexual associations and we used the snowball method to constitute our sample. The statistical significance level is P < 0.05.Results Almost half of the respondents (44.3%) were met with rejection by those around them. The majority (57.8%) have been victims of homophobic harassment. For Congolese population, homosexuality was perceived as a spiritual possession (90.5%). Homosexuality was not perceived as a handicap to undertake a profession (76.0%), although respondents with employment commitment represented just 15.3%.Conclusion The Congolese population has a bad perception of homosexuality, with an attitude of rejection and harassment towards LGBT people; in the other hand, homosexuals have a good perception of their homosexuality.
Geogas prospecting for igneous ore deposits covered by regolith: The Zijinshan high-sulfidation epithermal Cu-Au deposit in the Cathaysia Block
Authors: Li Quanheng , ye Rong , Duan Haichuan , Xu Kequan , Shen Shuoguo , Tian Yufeng 
Published: December 2023

Abstract
The mining industry faces a challenge due to the scarcity of outcrop or near-surface mineral deposits, necessitating the development of low-cost and efficient prospecting methods. Inductively coupled plasma mass spectrometry (ICP-MS) has been applied to geological sample analysis, and scientists have been gradually improving the method of geogas prospecting. As a result, geogas prospecting has shown promising results in detecting underlying concealed Au, U, and Cu-Ni deposits covered by Gobi sand and Quaternary sediments.To verify the effectiveness of this method for exploring underlying concealed mineral deposits developed in igneous covered areas, the Zijinshan high-sulfidation epithermal (HSE) Cu-Au deposit, a concealed mineral deposit in southeastern China, was selected as the experimental field. Our experiments revealed nanoscale particles composed of Au, Cu-Fe, and Cu-other elements in the geogas captured above the ore bodies of the deposit. Furthermore, Cu-nanoscale particles retain the isotopic composition of copper found in both the oxidation zone and deep copper ores. The geogas samples exhibited similar C1-chondrite normalized rare earth element (REE) distributions, with right-inclined REE patterns indicating significant enrichment of LREE relative to HREE, slightly negative δCe anomalies, and similar (La)N/(Sm)N and (Gd)N/(Yb)N ratios. These characteristics are similar to those of the gold ores, copper ores, altered rock, and bedrock near the ore bodies. These findings suggest that deep-penetration geochemical methods using geogas can be a valuable tool for uncovering underlying concealed mineral deposits in igneous covered areas.
Quantitative Research on the Disorder Degree of Traditional Settlements - A Case Study of Liangjia Village, Jingxing, Hebei Province
Authors: Peng Peng , Yating Fu , Zhou Xiangyun , Wu Shaopu , Zhao Jiajie , Zhang Yuechen 
Published: December 2023

Abstract

Traditional settlements are important material carriers of Chinese history and culture. After hundreds of years of development, the traditional settlements have survived until now, and the bottom-up construction mode reflects the most direct and original demands of the aborigines of all generations. The degree of disorder of the settlement, that is, the degree of its order, is not only the embodiment of its form, but also the concrete manifestation of its construction intention. To explore the intention behind the construction of settlements since ancient times.By using Delaunay triangulation network, street polar histogram and direction entropy, and comprehensibility of settlements, this paper quantitatively analyzes the degree of disorder in the three levels of architecture, street and local environment of the settlement. The paper, combining the background of the settlement, analyzes and compares the causes of the degree of disorder in the three levels of point, line and plane, and then explores the construction ideas of our ancestors for the settlement, so as to provide a scientific basis for the future planning of the settlements.Discovering the inherent construction principles within traditional settlements helps them maintain relative stability under external interventions. The results of disorder degree analysis serve as a benchmark for future settlement planning, contributing to the scientific basis of future decision-making.
A high-capacity Coverless Image Steganography Based on Rule based Machine Learning
Authors: KANZARIYA NITIN , Jadhav Dhaval 
Published: December 2023

Abstract
Securing private information during its transmission over the internet is of paramount importance, as it safeguards against unauthorized access and guarantees the integrity of data. Steganography serves as a technique for enhancing security by concealing sensitive information within digital content such as images, videos, audio, and textual carriers, thus thwarting unauthorized interception. Current methodologies involve manipulating pixels to create stego-images, effectively embedding confidential messages within the image structure. However, this approach has led to steganalysis experts uncovering these hidden messages. To address this issue, an innovative strategy for covert data concealment is proposed. This method involves integrating the secret message through the establishment of a cover file or secret message mapping. This approach ensures the confidentiality of sensitive data communicated over the internet. This research introduces a novel and highly-capable coverless image steganography technique utilizing RBML-based optical mark recognition. Notably, this approach surpasses previous alternatives in terms of embedding capacity, as substantiated by result analysis. Furthermore, it is adaptable for refinement as needed. Significantly, it exhibits remarkable resilience, safeguarding against a spectrum of attacks including scaling, color space conversion, JPEG compression, thresholding, "salt and pepper" noise, file format conversion, and steganalysis tools, among others.
Mapping the race between crop phenology and climate risks. A case-study for wheat in France under climate change conditions
Authors: Roux Renan Le , Furusho-Percot Carina , Deswarte Jean-Charles , Bancal Marie-Odile , Chenu Karine , Noblet-Ducoudré Nathalie , de Cortàzar-Atauri Iñaki García , Durand Alexis , Bulut Burak , Maury Olivier , Décome Jérémie , Launay Marie 
Published: December 2023

Abstract
Climate change threatens food security by affecting the productivity of major cereal crops. To date, agroclimatic risk projections have focused on expected hazards exposure during the crop’s current vulnerable seasons, without considering the non-stationarity of their phenology under evolving climatic conditions. We propose a new method for spatially classifying agroclimatic risks for wheat, combining high-resolution climatic data with a wheat’s phenological model. The method is implemented for French wheat involving three GCM-RCM model pairs and two emission scenarios. We found that the precocity of phenological stages allows wheat to avoid periods of water deficit in the near future. Nevertheless, in the coming decades under the RCP 8.5 scenario, climatic conditions for wheat cultivation are projected to deteriorate over the French territory, due to the emergence of heat stress and increasing water deficit. Projections show the appearance of combined risks of heat and water deficit up to 4 years per decade. The proposed method provides a deep level of information that enables regional adaptation strategies: the nature of the risk, its temporal and spatial occurrence, and its potential combination with other risks. It’s a first step towards identifying potential sites for breeding crop varieties to increase the resilience of agricultural systems.
Urine Biomarkers of Acute Kidney Injury and Association with Brain MRI Abnormalities in Hypoxic-Ischemic Encephalopathy
Authors: Turner Megan , Rumpel Jennifer , Spray Beverly , Stence Nicholas , Neuberger Ilana , Frymoyer Adam , Chock Valerie , Courtney Sherry , Gist Katja 
Published: December 2023

Abstract
Objective: Determine whether urine biomarkers NGAL (neutrophil gelatinase-associated lipocalin), KIM-1 (kidney injury molecule 1) and IL-18 (interleukin 18) are associated with abnormal MRI findings in neonates with hypoxic-ischemic encephalopathy (HIE) who underwent therapeutic hypothermia (TH).
Study Design: Secondary analysis of a multicenter, prospective study of neonates with HIE requiring TH. Urine biomarkers were obtained at 12 and 24 hours of life (HOL). Brain MRI was scored per NICHD criteria. Association between biomarkers and MRI stage was determined.
Results: In 57 neonates with HIE, only IL-18 at 24 HOL was significantly increased in neonates with MRI Stage 2B or greater, compared to Stage 2A or less (mean 398.7 vs. 182.9 pg/mL, p=0.024.) A multivariate model including IL-18 at 24 HOL and 5-minute APGAR performed best, with an AUC of 0.84 (SE=0.07, p=0.02).
Conclusions: Elevated urine IL-18 at 24 HOL was associated with more severe brain MRI abnormalities among neonates with HIE.