bluuebunny/crossref_metadata_2025_split huggingface dataset:
Signatures of Cysteine Oxidation on Muscle Structural and Contractile Proteins Are Associated with Physical Performance and Muscle Function in Older Adults: Study of Muscle, Mobility and Aging (SOMMA)
Authors: Day Nicholas J. , Kelly Shane S. , Lui Li-Yung , Mansfield Tyler A. , Gaffrey Matthew J. , Trejo Jesse B. , Sagendorf Tyler J. , Attah Kwame , Moore Ronald J. , Douglas Collin M. , Newman Anne B. , Kritchevsky Stephen B. , Kramer Philip A. , Marcinek David J. , Coen Paul M. , Goodpaster Bret H. , Hepple Russell T. , Cawthon Peggy M. , Petyuk Vladislav A. , Esser Karyn A. , Qian Wei-Jun , Cummings Steven R. 
Published: November 2023

AbstractOxidative stress is considered a contributor to declining muscle function and mobility during aging; however, the underlying molecular mechanisms remain poorly described. We hypothesized that greater levels of cysteine (Cys) oxidation on muscle proteins are associated with decreased measures of mobility. Herein, we applied a novel redox proteomics approach to measure reversible protein Cys oxidation in vastus lateralis muscle biopsies collected from 56 subjects in the Study of Muscle, Mobility and Aging (SOMMA), a community-based cohort study of individuals aged 70 years and older. We tested whether levels of Cys oxidation on key muscle proteins involved in muscle structure and contraction were associated with muscle function (leg power and strength), walking speed, and fitness (VO2peak on cardiopulmonary exercise testing) using linear regression models adjusted for age, sex, and body weight. Higher oxidation levels of select nebulin Cys sites were associated with lower VO2peak, while greater oxidation of myomesin-1, myomesin-2, and nebulin Cys sites was associated with slower walking speed. Higher oxidation of Cys sites in key proteins such as myomesin-2, alpha-actinin-2, and skeletal muscle alpha-actin were associated with lower leg power and strength. We also observed an unexpected correlation (r = 0.48) between a higher oxidation level of 8 Cys sites in alpha-actinin-3 and stronger leg power. Despite this observation, the results generally support the hypothesis that Cys oxidation of muscle proteins impair muscle power and strength, walking speed, and cardiopulmonary fitness with aging.
Investigating Antimicrobial Resistance Genes in Kenya, Uganda and Tanzania Cattle Using Metagenomics
Authors: Omar Kauthar M. , Kitundu George L. , Jimoh Adijat O. , Namikelwa Dorcus N. , Lisso Felix M. , Babajide Abiola A. , Olufemi Seun E. , Awe Olaitan I. 
Published: November 2023

AbstractAntimicrobial resistance (AMR) is a growing problem in African cattle production systems, posing a threat to human and animal health and the associated economic value chain. However, there is a poor understanding of the resistomes in small-holder cattle breeds in East African countries. This study aims to examine the distribution of Antimicrobial Resistance Genes (ARGs) in Kenya, Tanzania, and Uganda cattle using a metagenomics approach.We used the SqueezeMeta-Abricate (assembly-based) pipeline to detect ARGs and benchmarked this approach using the Centifuge-AMRplusplus (read-based) pipeline to evaluate its efficiency. Our findings reveal a significant number of ARGs of critical medical and economic importance in all three countries, including resistance to drugs of last resort such as carbapenems, suggesting the presence of highly virulent and antibiotic-resistant bacterial pathogens (ESKAPE) circulating in East Africa.Shared ARGs such as aph(6)-id (aminoglycoside phosphotransferase), tet (tetracycline resistance gene), sul2 (sulfonamide resistance gene) and cfxA_gen (betalactamase gene) were detected. Assembly-based methods revealed fewer ARGs compared to read-based methods, indicating the sensitivity and specificity of read-based methods in resistome characterization.Our findings call for further surveillance to estimate the intensity of the antibiotic resistance problem and wider resistome classification. Effective management of livestock and antibiotic consumption is crucial in minimizing antimicrobial resistance and maximizing productivity, making these findings relevant to stakeholders, agriculturists, and veterinarians in East Africa and Africa at large.
Specific and comprehensive genetic targeting reveals brain-wide distribution and synaptic input patterns of GABAergic axo-axonic interneurons
Authors: Raudales Ricardo , Kim Gukhan , Kelly Sean M. , Hatfield Joshua , Guan Wuqiang , Zhao Shengli , Paul Anirban , Qian Yongjun , Li Bo , Huang Z. Josh 
Published: November 2023

AbstractAxo-axonic cells (AACs), also called chandelier cells (ChCs) in the cerebral cortex, are the most distinctive type of GABAergic interneurons described in the neocortex, hippocampus, and basolateral amygdala (BLA). AACs selectively innervate glutamatergic projection neurons (PNs) at their axon initial segment (AIS), thus may exert decisive control over PN spiking and regulate PN functional ensembles. However, the brain-wide distribution, synaptic connectivity, and circuit function of AACs remains poorly understood, largely due to the lack of specific and reliable experimental tools. Here, we have established an intersectional genetic strategy that achieves specific and comprehensive targeting of AACs throughout the mouse brain based on their lineage (Nkx2.1) and molecular (Unc5b,Pthlh) markers. We discovered that AACs are deployed across essentially all the pallium-derived brain structures, including not only the dorsal pallium-derived neocortex and medial pallium-derived hippocampal formation, but also the lateral pallium-derived claustrum-insular complex, and the ventral pallium-derived extended amygdaloid complex and olfactory centers. AACs are also abundant in anterior olfactory nucleus, taenia tecta and lateral septum. AACs show characteristic variations in density across neocortical areas and layers and across subregions of the hippocampal formation. Neocortical AACs comprise multiple laminar subtypes with distinct dendritic and axonal arborization patterns. Retrograde monosynaptic tracing from AACs across neocortical, hippocampal and BLA regions reveal shared as well as distinct patterns of synaptic input. Specific and comprehensive targeting of AACs facilitates the study of their developmental genetic program and circuit function across brain structures, providing a ground truth platform for understanding the conservation and variation of a bona fide cell type across brain regions and species.
MSGene: Derivation and validation of a multistate model for lifetime risk of coronary artery disease using genetic risk and the electronic health record
Authors: Urbut Sarah M. , Yeung Ming Wai , Khurshid Shaan , Jemma Cho So Mi , Schuermans Art , German Jakob , Taraszka Kodi , Fahed Akl C. , Ellinor Patrick , Trinquart Ludovic , Parmigiani Giovanni , Gusev Alexander , Natarajan Pradeep 
Published: November 2023

AbstractCurrently, coronary artery disease (CAD) is the leading cause of death among adults worldwide. Accurate risk stratification can support optimal lifetime prevention. We designed a novel and general multistate model (MSGene) to estimate age-specific transitions across 10 cardiometabolic states, dependent on clinical covariates and a CAD polygenic risk score. MSGene supports decision making about CAD prevention related to any of these states. We analyzed longitudinal data from 480,638 UK Biobank participants and compared predicted lifetime risk with the 30-year Framingham risk score. MSGene improved discrimination (C-index 0.71 vs 0.66), age of high-risk detection (C-index 0.73 vs 0.52), and overall prediction (RMSE 1.1% vs 10.9%), with external validation. We also used MSGene to refine estimates of lifetime absolute risk reduction from statin initiation. Our findings underscore the potential public health value of our novel multistate model for accurate lifetime CAD risk estimation using clinical factors and increasingly available genetics.
WITHDRAWN: Genome-wide risk prediction of primary open-angle glaucoma across multiple ancestries
Authors: Gharahkhani Puya , He Weixiong , Han Xikun , Ong Jue Sheng , Rentería Miguel E , Wiggs Janey L , Khawaja Anthony P , Trzaskowski Maciej , Mackey David A. , Craig Jamie E. , Hewitt Alex W. ,   IGGC International Glaucoma Genetics Consortium, MacGregor Stuart , Wu Yeda 
Published: November 2023

Withdrawal statementThis manuscript has been withdrawn by medRxiv following a formal request by the QIMR Berghofer Medical Research Institute Research Integrity Office owing to lack of author consent.
Responsible leadership toward garnering legitimacy from stakeholders in India: an institutional perspective
Authors: Sargam Sadhna , Pandey Ashish 
Published: November 2023

PurposeUnderstanding societal expectations of a leader's responsibility and context-specific challenges in less-researched emerging economies has become imperative for foreign Multinational enterprises (MNEs) to survive in these contexts while developing globally responsible leaders. Identification of institutionally sanctioned characteristics, competencies and strategies that assist leaders in dealing with such challenges while achieving shared value has wider implications for academics, practitioners and the literature on responsible leadership (RL), which is the purpose of this paper.Design/methodology/approachThe authors conducted in-depth interviews of 28 senior indigenous leaders in 3 Indian Multinational corporations (MNCs) in construction sector, following a grounded theory approach.FindingsThe authors identified three vital institutionally driven challenges and four individual-level societal-driven factors, subsequently influencing leaders' strategic decisions and choices to deal with such challenges beyond passive conformance. Contrary to the previous findings, this study also briefly discusses that a mere ethical climate is insufficient; organizations must develop a holistic values climate that works as contextual factors to influence RL.Originality/valueContrary to the previous findings suggesting Indian leaders' conformance to constraining forces to RL, by adopting a multilevel approach, the authors identify the context-specific strategic behaviors that responsible leaders adopt in dealing with such forces responsibly. Thus, it is the first multilevel inductive approach conducted in a non-Western context, offering a discrete understanding of RL while addressing some of the inconsistencies in the literature and contributing to cross-cultural leadership research. Also, findings highlight the factors of RL that are more emic and etic for generalization.
Mobile work, individual aspirations and job satisfaction in Europe
Authors: Curzi Ylenia , Pistoresi Barbara , Coppeta Gaetano Francesco 
Published: November 2023

PurposeThis article responds to the call for more research on mobile work by exploring how the aspirations of these workers relate to job satisfaction through adaptation to the job characteristics they experience.Design/methodology/approachBased on aspiration theory and the literature on mobile work, the paper examines how mobile workers form aspirations and how this is related to their perception of job satisfaction. The empirical analysis uses a two-tier stochastic frontier analysis and the 2015 European Working Conditions Survey dataset.FindingsMobile workers formulate higher aspirations than the working conditions they experience and report lower levels of job satisfaction than other types of workers. They revise their aspirations downwards when they experience autonomy, discretion, performance-related pay schemes, relation-oriented leadership while they increase their aspirations when they experience work intensification and discrimination.Originality/valueThis paper provides new insights into the work perceptions of mobile workers and enriches existing research by highlighting the importance of the study of individual aspirations to advance understanding of the complex dynamics of mobile work.
Influence of elevated temperature on buckling capacity of mild steel-based cold-formed steel column sections– experimental investigation and finite element modelling
Authors: Sabu Sam Varun , Adarsh M.S. , Lyngdoh Garry Robson , Marak Garry Wegara K. , Anand N. , Al-Jabri Khalifa , Andrushia Diana 
Published: November 2023

PurposeThe capability of steel columns to support their design loads is highly affected by the time of exposure and temperature magnitude, which causes deterioration of mechanical properties of steel under fire conditions. It is known that structural steel loses strength and stiffness as temperature increases, particularly above 400 °C. The duration of time in which steel is exposed to high temperatures also has an impact on how much strength it loses. The time-dependent response of steel is critical when estimating load carrying capacity of steel columns exposed to fire. Thus, investigating the structural response of cold-formed steel (CFS) columns is gaining more interest due to the nature of such structural elements.Design/methodology/approachIn this study, experiments were conducted on two CFS configurations: back-to-back (B-B) channel and toe-to-toe (T-T) channel sections. All CFS column specimens were exposed to different temperatures following the standard fire curve and cooled by air or water. A total of 14 tests were conducted to evaluate the capacity of the CFS sections. The axial resistance and yield deformation were noted for both section types at elevated temperatures. The CFS column sections were modelled to simulate the section's behaviour under various temperature exposures using the general-purpose finite element (FE) program ABAQUS. The results from FE modelling agreed well with the experimental results. Ultimate load of experiment and finite element model (FEM) are compared with each other. The difference in percentage and ratio between both are presented.FindingsThe results showed that B-B configuration showed better performance for all the investigated parameters than T-T sections. A noticeable loss in the ultimate strength of 34.5 and 65.6% was observed at 90 min (986℃) for B-B specimens cooled using air and water, respectively. However, the reduction was 29.9 and 46% in the T-T configuration, respectively.Originality/valueThis research paper focusses on assessing the buckling strength of heated CFS sections to analyse the mode of failure of CFS sections with B-B and T-T design configurations under the effect of elevated temperature.
A novel cascaded transformer coupled multilevel inverter with reduced number of switches for high power applications
Authors: Behera Rashmi Rekha , Dash Ashish Ranjan , Panda Anup Kumar 
Published: November 2023

Purpose
The purpose of this paper is to design a cascaded Multilevel inverter with reduce number of switches for high power applications. This paper came up with an innovative three-phase multilevel inverter (MLI) topology, which is a cascaded structure based on classical three-legged voltage source inverter (VSI) bridges as an individual module. The prominent advantage of this topology is that it requires only one direct current (DC) link system. The main characteristic of it is that a higher number of voltage levels can be achieved with considerably a smaller number of semiconductor switches, which improves the reliability, power quality, cost and size of the system significantly.

Design/methodology/approach
The individual modules are cascaded through three-phase transformers to provide higher voltage at the output with the higher number of voltage levels. In this work, the phase-shifted pulse width modulation technique is implemented to verify the result.

Findings
The proposed topology is compared with three-phase cascaded H-bridge MLI (CHB-MLI) and a modified CHB-MLI topology and found better in many aspects. The proposed MLI can produce a higher number of voltage levels with fewer semiconductor switches and associated triggering circuitry. As the device count in the proposed MLI is less compared to other MLI discussed, it tends to have less switching and conduction loss which increases the efficiency and reliability. As the number of level increases, the voltage profile and the total harmonic distortion of the proposed MLI improves.

Originality/value
This is a transformer-based modular cascaded MLI, which is based on classical VSI bridges. Here in this topology, a single module provides all three phases. So, a single string of cascaded modules is enough for three-phase multilevel voltage generation.
Death after endoscopic prostate surgery in Australia and New Zealand: a review of clinical management issues
Authors: Galiabovitch Elena , Lim Kylie Yen‐Yi , McCahy Philip 
Published: November 2023

AbstractBackgroundEndoscopic prostate surgery is an established treatment for male lower urinary tract symptoms (LUTS) and is recognized to have low mortality rates. We aimed to describe factors that may have contributed to death following endoscopic prostate surgery using data from the Australia and New Zealand Audits of Surgical Mortality (ANZASM).MethodsAll urological related deaths reported to ANZASM from January 1 2012 to December 31 2019 were reviewed. Deaths related to endoscopic prostate resection (transurethral resection of prostate – TURP, laser procedures) were included. Peer reviewers identified up to three clinical management issues (CMIs), and these were analysed. CMIs were classified in order of least to most concerning: area of consideration, area of concern and adverse events.ResultsOf 1127 total urological deaths, 77 deaths were related to endoscopic prostate surgery (7.0%). Most procedures were monopolar TURP. The mean age of patients was 80.9 years (range 57.0–96.2). Leading causes of death were cardiovascular events 23/77 (29.9%) and respiratory failure 14/77 (18.2%). Assessors identified 39 CMIs in 26/77 (33.8%) patients. Twenty‐three were areas of consideration, nine were areas of concern and seven identified adverse events. The most common CMIs were regarding post‐operative care (14/39) and the decision to operate (13/39).ConclusionMost deaths did not elicit concerns from the ANZASM peer assessors. However, the main concerns identified were surrounding decision making and rationale for operating. This highlights the importance of clear counselling and documentation during the treatment decision process.
Gilteritinib‐based combination therapy in adult relapsed/refractory FLT3‐mutated acute myeloid leukaemia
Authors: Chen Nianci , Pan Jiajia , Zhou Yile , Mao Liping , Lou Yinjun , Qian Jiejing , Xu Gaixiang , Wei Juying , Zhou De , Shou Lihong , Huang Li , Yan Minchao , Zeng Hui , Fan Cuihua , Wu Gongqiang , Feng Weiying , Tong Hongyan , Jin Jie , Wang Huafeng 
Published: November 2023

SummaryGilteritinib, a potent FMS‐like tyrosine kinase 3 (FLT3) inhibitor, was approved for relapsed/refractory (R/R) FLT3‐mutated acute myeloid leukaemia (AML) patients but still showed limited efficacy. Here, we retrospectively analysed the efficacy and safety of different gilteritinib‐based combination therapies (gilteritinib plus hypomethylating agent and venetoclax, G + HMA + VEN; gilteritinib plus HMA, G + HMA; gilteritinib plus venetoclax, G + VEN) in 33 R/R FLT3‐mutated AML patients. The composite complete response (CRc) and modified CRc (mCRc) rates were 66.7% (12/18) and 88.9% (16/18) in patients received G + HMA + VEN, which was higher compared with that in G + HMA (CRc: 18.2%, 2/11; mCRc: 45.5%, 5/11) or G + VEN (CRc: 50.0%, 2/4; mCRc: 50.0%, 2/4). The median overall survival (OS) for G + HMA + VEN, G + HMA and G + VEN treatment was not reached, 160.0 days and 231.0 days. The median duration of remission (DOR) for G + HMA + VEN, G + HMA and G + VEN treatment was not reached, 82.0 days and 77.0 days. Four patients in the G + HMA + VEN group received alloHSCT after remission exhibited prolonged median DOR. The most common grade 3/4 adverse events were cytopenia, febrile neutropenia and pulmonary infection; there were no differences among the three groups. In conclusion, our data demonstrated promising response of G + HMA + VEN combination therapy in R/R FLT3‐mutated AML, and it may be considered an effective therapy bridge to transplantation.
Skin sensitization data for methyl esters of sulphonic acids—Corrections to published databases required
Authors: Roberts David W. 
Published: November 2023

AbstractBackgroundSeveral methyl esters of sulphonic acids are listed in murine local lymph node assay (LLNA) databases, with dose–response data and EC3 values. However, some of these entries are questionable—in one case the chemical tested is not the chemical named in the databases and in others the EC3 value has been derived by extrapolation from data that do not meet the applicability criteria for the approved extrapolation method.ObjectivesTo consider how LLNA data came to be attributed to the wrong chemical and to address the inappropriate extrapolated EC3 values.MethodsDose–response data for methyl hexadec‐3‐enesulphonate (wrongly named as methyl hexadec‐1‐enesulphonate), two other methyl sulphonates and hexadec‐1‐ene‐1,3‐sultone are re‐evaluated using the single dose probit extrapolation method (SDPEM). The different reaction chemistry profiles of methyl hexadec‐3‐enesulphonate and methyl hexadec‐1‐enesulphonate are discussed.ResultsExtrapolated EC3 values for hexadec‐1‐ene‐1,3‐sultone are the same by both methods but for the methyl sulphonates the differences are substantial.ConclusionsCurrent databases should be corrected and further analysed to identify other cases where EC3 values are likely to be unreliable due to inappropriate estimation by extrapolation.
Plastic film mulching maintains soil organic carbon by increasing fungal necromass carbon under manure application
Authors: Liu Xu , Bol Roland , Ge Zhuang , Ma Nan , Li Tingyu , Liu Yaocen , Li Shuangyi , An Tingting , Wang Jingkuan 
Published: November 2023

AbstractMicrobial necromass carbon (MNC) is a major contributor to soil organic carbon (SOC) sequestration. Fertilization combined with plastic film mulching, as an intensive agricultural practice to increase crop yields, affects soil microbial growth and metabolism. Nevertheless, how fertilization combined with mulching affects SOC sequestration by mediating MNC dynamics remains elusive. Here, the mulching and no‐mulching sub‐treatments were set under three fertilization treatments (no fertilization, NF; inorganic fertilization, IF; manure, MF), and a 900‐day field incubation experiment using polyvinyl chloride containers was conducted in the buffer zone of NF treatment. We investigated the effects of fertilization combined with mulching on MNC composition (including fungal necromass carbon, FNC; and bacterial necromass carbon, BNC) and qualified their contributions to SOC sequestration. The MF treatment with/without mulching significantly increased the contents of MNC, FNC, BNC, and SOC by 97%–122%, 81%–94%, 152%–210%, and 60%–70% compared with the NF treatment without mulching over 900 days, respectively. The MNC content had a strongly positive correlation with particulate organic carbon and microbial biomass C (p < 0.01). During the incubation stage, the proportion of MNC in SOC was higher in the IF (37%–42%) and MF (40%–44%) treatments with/without mulching than that in the NF treatment with/without mulching (31%–35%). On day 900, mulching significantly decreased the MNC content by 8.7% and 7.8%, and decreased the proportion of MNC in SOC by 5.1% and 5.8% under the NF and IF treatments, respectively. In contrast, mulching did not significantly affect the MNC and SOC contents, but significantly increased the FNC content by 4.8% under the MF treatment on day 900. Mulching significantly decreased the proportion of BNC in SOC regardless of fertilization, and increased the proportion of FNC in SOC under the MF treatment on day 900. Overall, our findings suggest that mulching under manure application maintains SOC sequestration by promoting FNC retention.