bluuebunny/crossref_metadata_2025_split huggingface dataset:
m6A Methylated Long Noncoding RNA LOC339803 Regulates Intestinal Inflammatory Response
Authors: Olazagoitia‐Garmendia Ane , Rojas‐Márquez Henar , Sebastian‐delaCruz Maialen , Agirre‐Lizaso Aloña , Ochoa Anne , Mendoza‐Gomez Luis Manuel , Perugorria Maria J , Bujanda Luis , Madrigal Alain Huerta , Santin Izortze , Castellanos‐Rubio Ainara 
Published: January 2024

AbstractCytokine mediated sustained inflammation increases the risk to develop different complex chronic inflammatory diseases, but the implicated mechanisms remain unclear. Increasing evidence shows that long noncoding RNAs (lncRNAs) play key roles in the pathogenesis of inflammatory disorders, while inflammation associated variants are described to affect their function or essential RNA modifications as N6‐methyladenosine (m6A) methylation, increasing predisposition to inflammatory diseases. Here, the functional implication of the intestinal inflammation associated lncRNA LOC339803 in the production of cytokines by intestinal epithelial cells is described. Allele‐specific m6A methylation is found to affect YTHDC1 mediated protein binding affinity. LOC339803‐YTHDC1 interaction dictates chromatin localization of LOC339803 ultimately inducing the expression of NFκB mediated proinflammatory cytokines and contributing to the development of intestinal inflammation. These findings are confirmed using human intestinal biopsy samples from different intestinal inflammatory conditions and controls. Additionally, it is demonstrated that LOC339803 targeting can be a useful strategy for the amelioration of intestinal inflammation in vitro and ex vivo. Overall, the results support the importance of the methylated LOC339803 lncRNA as a mediator of intestinal inflammation, explaining genetic susceptibility and presenting this lncRNA as a potential novel therapeutic target for the treatment of inflammatory intestinal disorders.
Stimulus‐Responsive Copper Complex Nanoparticles Induce Cuproptosis for Augmented Cancer Immunotherapy
Authors: Hu Fuzhen , Huang Jia , Bing Tiejun , Mou Wenlong , Li Duo , Zhang Hanchen , Chen Yang , Jin Qionghua , Yu Yingjie , Yang Zhiying 
Published: January 2024

AbstractCuproptosis, an emerging form of programmed cell death, has received tremendous attention in cancer therapy. However, the efficacy of cuproptosis remains limited by the poor delivery efficiency of copper ion carriers. Herein, copper complex nanoparticles (denoted as Cu(I) NP) are developed that can efficiently deliver copper complex into cancer cells to induce cuproptosis. Cu(I) NP demonstrate stimulus‐responsive release of copper complexes, which results in mitochondrial dysfunction and promotes the aggregation of lipoylated dihydrolipoamide S‐acetyltransferase (DLAT), leading to cuproptosis. Notably, Cu(I) NP not only induce cuproptosis, but also elicit robust immune responses to suppress tumor growth. Overall, this study provides a promising strategy for cuproptosis‐based cancer therapy.
Operando Mobile Catalysis for Reverse Water Gas Shift Reaction
Authors: Liang Haojie , Zhang Bin , Hong Mei , Yang Xinchun , Zhu Ling , Liu Xingchen , Qi Yuntao , Zhao Shichao , Wang Guofu , van Bavel Alexander P. , Wen Xiaodong , Qin Yong 
Published: January 2024

AbstractMetal atoms on the support serve as active sites for many heterogeneous catalysts. However, the active metal sites on the support are conventionally described as static, and the intermediates adsorbed on the support far away from the active metal sites cannot be transformed. Herein, we report the first example of operando mobile catalysis to promote catalytic efficiency by enhancing the collision probability between active sites and reactants or reaction intermediates. Specifically, ligand‐coordinated Pt single atoms (isolated MeCpPt‐ species) are bonded on CeO2 and transformed into mobile MeCpPt(H)CO complexes during the reverse water gas shift reaction for operando mobile catalysis. This strategy enables the conversion of inert carbonate intermediates on the CeO2 support. A turnover frequency (TOF) of 6358 mol CO2 molPt−1 ⋅ h−1 and 99 % CO selectivity at 300 °C is obtained for reverse water gas shift reaction, dramatically higher than those of Pt catalysts reported in the literature. Operando mobile catalysis presents a promising strategy for designing high‐efficiency heterogeneous catalysts for various chemical reactions and applications.
Ups and downs: An insight on the stranding pattern of Guiana dolphins, Sotalia guianensis, in the Amazon Estuary, Northern Brazil
Authors: Ruenes Greicy F. , Costa Alexandra F. , Laeta Maíra , Emin‐Lima Renata , Oliveira Larissa R. , Siciliano Salvatore 
Published: January 2024

Abstract
Long‐term systematic records of cetacean strandings are valuable indicators of temporal responses of specific cetacean populations and communities to natural or anthropogenic pressures. They also help guide management objectives and conservation actions.
Generalized additive models (GAMs) were used to analyse 10 years of systematic and opportunistic surveys of Guiana dolphin strandings in the Amazon Estuary region, Northern Brazil. The spatial distribution of strandings was assessed through kernel density maps, and age‐at‐death structure of this population was described.
The model results revealed marked seasonality in the strandings and, by extension, the mortality of Guiana dolphins in the Amazon Estuary. This seasonality varied between the inner estuarine regions and Atlantic coastal area, reflecting the environment's characteristics. Long‐term decline of strandings in the Amazon Estuary inner region and unusual high mortality of young sexually mature individuals were detected. These results, besides the high stranding rate detected for the species, are mostly attributed to the species' interaction with intense fishing activity in the region.
Conservation efforts should consider the seasonality of Guiana dolphin strandings in the Amazon Estuary. The systematic monitoring of strandings, especially in high‐frequency areas, is recommended for assessing long‐term species mortality. Additionally, implementing management actions related to the species' interaction with fishing activities, such as regulatory enhancements, gillnet modifications and seasonal no‐fishing zones, is advised. Educational campaigns targeting fishermen and riverine communities are essential to reduce bycatch and the folkloric use of the species.
This study underscores the effectiveness of stranding data as a source of biological and ecological information, and as a tool for understanding human actions affecting coastal populations of aquatic mammals. It emphasizes the need for urgent studies to characterize this population, clarify the influence of bycatch as an anthropogenic pressure and implement effective conservation actions to preserve the Guiana dolphin population.
Differential expression of brummer and levels of TAG in different developmental stages Aedes aegypti (Diptera: Culicidae), including fasted adults
Authors: dos Santos Luan Valim , Silva Elaine Rodrigues Miranda Nery da , Caiado Matheus Silva , Rezende Sabrina Rita da Fonseca , de Carvalho Mario Geraldo , Pontes Emerson Guedes 
Published: January 2024

AbstractLipid storage in the form of triacylglycerol (TAG) is essential for insect life, as it enables flight, development, and reproduction. The activity of the lipase brummer (bmm) has been shown to be essential to insects' homeostasis. The objective of this study was to evaluate how bmm expression occurs in Aedes aegypti larvae and adults, and to observe TAG levels during fasting in adult females. The bmm sequence was identified in A. aegypti and exhibited a patatin‐like phospholipase domain reinforced by the presence of a catalytic dyad with serine and aspartate residues, revealing a high degree of similarity with other organisms. Bmm expression was differentiated in the larvae and adult fat body (FB) following TAG reserve dynamics. Bmm was expressed three times in larval stages L3, L4, and pupae compared with L1 and L2, which could indicate its role in the maturation of these insects. In the postemergence (PE) and post‐blood meal (PBM) FB of adult insects, bmm expression varied over several days. PE adults showed a pronounced bmm increase from the third day onward compared with those not subjected to fasting. This was accompanied by a decrease in TAG from the third day onward, suggesting the participation of bmm. Six hours after blood feeding, TAG levels increased in mosquitos reared in the absence of sucrose, suggesting lipid accumulation to guarantee reproduction. Bmm responded positively to fasting, followed by TAG mobilization in adult FB. During the previtellogenic period, bmm levels responded to low TAG levels, unlike the PBM period.
Plasma Matrix Metalloproteinase Concentrations and Risk of Interstitial Lung Disease in a Prospective Rheumatoid Arthritis Cohort
Authors: Luedders Brent A. , Wheeler Austin M. , Ascherman Dana P. , Baker Joshua F. , Duryee Michael J. , Yang Yangyuna , Roul Punyasha , Wysham Katherine D. , Monach Paul , Reimold Andreas , Kerr Gail S. , Kunkel Gary , Cannon Grant W. , Poole Jill A. , Thiele Geoffrey M. , Mikuls Ted R. , England Bryant R. 
Published: January 2024

ObjectiveTo evaluate the associations of plasma matrix metalloproteinases (MMPs) with prevalent and incident interstitial lung disease (ILD) in people with rheumatoid arthritis (RA).MethodsWithin a multicenter, prospective cohort of US veterans with RA, we performed a cross‐sectional study of prevalent ILD and cohort study of incident ILD. ILD diagnoses were validated by medical record review of provider diagnoses and chest imaging and/or pathology reports. MMP‐1, 3, 7, and 9 concentrations were measured in plasma samples, then standardized and categorized into quartiles. The associations of MMPs with prevalent and incident ILD were assessed with logistic (prevalent) and Cox (incident) regression models adjusted for RA‐ILD risk factors.ResultsAmong 2,312 participants (88.9% male; mean age 63.8 years), 96 had prevalent ILD. Incident ILD developed in 130 participants over 17,378 person‐years of follow‐up (crude incidence rate 7.5/1,000 person‐years). Participants with the highest quartile of MMP‐7 concentrations had a nearly four‐fold increased odds of prevalent ILD (adjusted odds ratio 3.78 [95% confidence interval (95% CI) 1.86–7.65]) and over two‐fold increased risk of incident ILD (adjusted hazard ratio 2.33 [95% CI 1.35–4.02]). Higher MMP‐9 concentrations were also associated with prevalent and incident ILD, as well as negatively correlated with forced vital capacity among those with prevalent ILD (r = −0.30, P = 0.005).ConclusionMMP‐7 and MMP‐9 were strongly associated with both prevalent and incident ILD in this large, multicenter RA cohort after adjustment for other RA‐ILD risk factors. These population‐level findings further support a potential pathogenic role for MMPs in RA‐ILD and suggest that their measurement could facilitate RA‐ILD risk stratification.
Predisposal of Interferon Regulatory Factor 1 Deficiency to Accumulate DNA Damage and Promote Osteoarthritis Development in Cartilage
Authors: Cho Yongsik , Kim Hyeonkyeong , Yook Geunho , Yong Sangmin , Kim Soy , Lee Narae , Kim Yi‐Jun , Kim Jin‐Hee , Kim Tae Woo , Chang Moon Jong , Lee Kyoung Min , Chang Chong Bum , Kang Seung‐Baik , Kim Jin‐Hong 
Published: January 2024

ObjectiveInterferon regulatory factor 1 (IRF1) is a transcriptional regulator conventionally associated with immunomodulation. Recent molecular analyses mapping DNA binding sites of IRF1 have suggested its potential function in DNA repair. However, the physiologic significance of this noncanonical function remains unexplored. Here, we investigated the role of IRF1 in osteoarthritis (OA), a condition marked by senescence and chronic joint inflammation.MethodsOA progression was examined in wild‐type and Irf1−/− mice using histologic assessments and microcomputed tomography analysis of whole‐joint OA manifestations and behavioral assessments of joint pain. An integrated analysis of assay for transposase‐accessible chromatin with sequencing and whole transcriptome data was conducted for the functional assessment of IRF1 in chondrocytes. The role of IRF1 in DNA repair and senescence was investigated by assaying γ‐H2AX foci and senescence‐associated beta‐galactosidase activity.ResultsOur genome‐wide investigation of IRF1 footprinting in chondrocytes revealed its primary occupancies in the promoters of DNA repair genes without noticeable footprint patterns in those of interferon‐responsive genes. Chondrocytes lacking IRF1 accumulated irreversible DNA damage under oxidative stress, facilitating their entry into cellular senescence. IRF1 was down‐regulated in the cartilage of human and mouse OA. Although IRF1 overexpression did not elicit an inflammatory response in joints or affect OA development, genetic deletion of Irf1 caused enhanced chondrocyte senescence and exacerbated post‐traumatic OA in mice.ConclusionIRF1 offers DNA damage surveillance in chondrocytes, protecting them from oxidative stress associated with OA risk factors. Our study provides a crucial and cautionary perspective that compromising IRF1 activity renders chondrocytes vulnerable to cellular senescence and promotes OA development.image
A Cationic Catechol Derivative Binds Anions in Competitive Aqueous Media
Authors: Goodwin Rosemary J. , White Nicholas G. 
Published: January 2024

AbstractA simple dihydroxy isoquinolinium molecule (3+) was prepared by a modification of a literature procedure. Interestingly, during optimisation of the synthesis a small amount of the natural product pseudopalmatine was isolated, and characterised for the first time by X‐ray crystallography. Compound 3+ contains a catechol motif and positive charge on the same scaffold and was found to be a potent anion receptor, binding sulfate strongly in 8 : 2 d6‐acetone:D2O and 7 : 3 d6‐acetone:D2O (Ka>104 and 2,100 M−1, respectively). Unsurprisingly, chloride binding was much weaker, even in the less polar solvent mixture 9 : 1 d6‐acetone:D2O. The sulfate binding is remarkably strong for such a simple molecule, however anion binding studies were complicated by the tendency of the molecule to react with BPh4− or BF4− species during anion metathesis reactions. This gave two unusual zwitterions containing tetrahedral boronate centres, which were both characterised by X‐ray crystallography.
Protocol for the development and validation of a clinical measurement tool for fear of disease progression and recurrence in cardiac patients
Authors: Clarke Sarah T. , Le Grande Michael , Murphy Barbara M. , Hester Robert , Jackson Alun C. 
Published: January 2024

AbstractIntroductionOne in two cardiac patients fear having another heart event or their heart condition getting worse. Research in other chronic illnesses demonstrates that screening for fear of progression and recurrence is vital for adequately addressing such concerns in clinical care. The current project aims to develop and validate a measure for fear of progression and recurrence in cardiac patients.MethodsThe Fear of Cardiac Recurrence and Progression Scale (FCRP) will be developed through a multistep process. An initial item pool will be generated through a review of the literature and existing measures and consultation with and feedback from key informants. The item pool will be tested in a sample of over 250 adults who have ever had an acute coronary event, undergone cardiac surgery, or a chronic cardiac condition. Exploratory factor analysis will be used to identify the underlying factors, and Rasch analysis will be used to reduce the number of items. A short form version of the FCRP will be developed for use as a brief screening tool, informed by clinical relevance and Rasch psychometric indices.DiscussionWhile many cardiac patients experience fears related to the progression or recurrence of their illness, there remains the need for a validated tool with which these concerns can be identified and measured. It is expected that the design and validation of the FCRP will aid identification of cardiac patients suffering from clinically significant levels of fear of progression and recurrence and facilitate the design of tailored psychological interventions to target these fears.
Continuous Synthesis of a Macrocyclic Sulfite of Polyethylene Glycol by Cascaded Continuous Stirred Tank Reactors (CSTRs)
Authors: Wei Jichang , Liu Yinli , Sun Yuchen , Bai Jun , Gao He , Yang Zhaojun , Pan Long 
Published: January 2024

AbstractMany macrocyclic compounds are attractive drug‐like molecules or intermediates due to their special properties. However, the bulk synthesis of such compounds are hindered by the necessity of using diluted solutions, in order to prevent intermolecular reactions that yields oligomer impurities, thereby resulting in a low production efficiency. Such challenge can be adequately addressed by using continuous reactors, allowing improved efficiency with smaller space footprints. In this work, we proposed a novel continuous process for the synthesis of a macrocyclic sulfite of tetraethylene glycol (PEG4‐MCSi), which is a precursor to a very useful building block, PEG4‐macrocyclic sulfate (PEG4‐MCS). The basic reaction parameters, including stoichiometry and temperature, were first confirmed with small batch reactions, and the effectiveness of coiled reactors and continuous stirred tank reactors (CSTRs) were compared. Cascaded CSTRs were proven to be suitable, and the reaction parameters were subject to further optimization to give a robust continuous process. The process was then tested with 4 parallel runs for up to 64 h. Finally, the merits and demerits of batch and continuous reactions were also compared, demonstrating the suitability of latter in the bulk production of macrocyclic PEG‐MCSi compounds.
Combined chemotherapy of zoledronic acid and pamidronate in the treatment of bone metastases from nonsmall cell lung cancer and the effects on pain stress and bone metabolic indices
Authors: Huang Kun , Tang Xiao , Tang Fang 
Published: January 2024

AbstractObjectiveWe conducted this paper to decipher the efficacy of the combined chemotherapy of zoledronic acid and pamidronate in treating bone metastases from nonsmall cell lung cancer (NSCLC) and the effects on pain stress and bone metabolic indices.MethodsPatients with bone metastases from NSCLC were allocated into Group A and Group B. Patients in the Group A were administrated with pamidronate combined chemotherapy and patients in the Group B were administrated with zoledronic acid combined chemotherapy. The efficacy, pain symptom scores, quality of life scores, serum inflammatory factor, serum bone metabolic indices, serum pain stress indicators, and the occurrence of adverse effects were compared in patients of the two groups.ResultsThe total effective rate of treatment was higher in the Group B than in the Group A. After treatment, reduced Numerical Rating Scale scores and elevated Karnofsky Performance Score score, reduced serum levels of N‐terminal mid‐fragment of osteocalcin, N‐terminal propeptide of type I procollagen, bone‐specific alkaline phosphatase, and type I collagen hydroxyl terminal peptide β special sequence, reduced serum levels of C‐reactive protein, procalcitonin, tumor necrosis factor‐α, and interleukin‐6, as well as decreased levels of bradykinin, substance P, neuropeptide Y, and β‐endorphin were found in the Group B versus the Group A. No notable difference was witnessed in the rate of adverse reactions between the Group A and the Group B.ConclusionZoledronic acid combined with chemotherapy can effectively treat bone metastases of NSCLC and improve pain stress and bone metabolic status, which has value that can be promoted and applied in clinical treatment.
Specialist reassociation and residence time modulate the evolution of defense in invasive plants: A meta‐analysis
Authors: Yi Jiahui , Wan Jinlong , Tielbörger Katja , Tao Zhibin , Siemann Evan , Huang Wei 
Published: January 2024

AbstractInvasive plants typically escape specialist herbivores but are often attacked by generalist herbivores in their introduced ranges. The shifting defense hypothesis suggests that this will cause invasive plants to evolve lower resistance against specialists, higher resistance against generalists, and greater tolerance to herbivore damage. However, the duration and direction of selective pressures can shape the evolutionary responses of resistance and tolerance for invasive plants. Two critical factors are (1) residence time (length of time that an invasive species has been in its introduced range) and (2) specialist herbivore reassociation (attack by purposely or accidentally introduced specialists). Yet, these two factors have not been considered simultaneously in previous quantitative syntheses. Here, we performed a meta‐analysis with 367 effect sizes from 70 studies of 35 invasive plant species from native and invasive populations. We tested how the residence time of invasive plant species and specialist reassociation in their introduced ranges affected evolutionary responses of defenses against specialists and generalists, including herbivore resistance traits (physical barriers, digestibility reducers and toxins), resistance effects (performance of and damage caused by specialists or generalists) and tolerance to damage (from specialists or generalists). We found that residence time and specialist reassociation each significantly altered digestibility reducers, specialist performance, generalist damage, and tolerance to specialist damage. Furthermore, residence time and specialist reassociation strongly altered toxins and generalist performance, respectively. When we restricted consideration to invasive plant species with both longer residence times and no reassociation with specialists, invasive populations had lower resistance to specialists, similar resistance to generalists, and higher tolerance to damage from both herbivore types, compared with native populations. We conclude that the duration and direction of selective pressure shape the evolutionary responses of invasive plants. Under long‐term (long residence time) and stable (no specialist reassociation) selective pressure, invasive plants generally decrease resistance to specialists and increase tolerance to generalist damage that provides mixed support for the shifting defense hypothesis.
Water Adducts of the Lewis Superacids: Tris(pentafluoroethyl)gallane and ‐indane
Authors: Tölke Katharina , Porath Sven , Vishnevskiy Yury V. , Neumann Beate , Stammler Hans‐Georg , Mitzel Norbert W. , Hoge Berthold 
Published: January 2024

AbstractLewis superacids (LSA) are defined by a fluoride ion affinity (FIA) that exceeds that of SbF5, and are thus characterized as hard according to the HSAB concept. Soft superacidity was defined for compounds exceeding the hydride ion affinity (HIA) of B(C6F5)3. Herein we report on the synthesis of a Lewis acid being superacidic only on the soft regime: tris(pentafluoroethyl)indane, In(C2F5)3. It is accessible in the form of its dihydrate [In(C2F5)3(OH2)2]. An improved synthesis for the gallium analogue [Ga(C2F5)3(OH2)2] is also reported. Tris(pentafluoroethyl)indane dihydrate, [In(C2F5)3(OH2)2], was fully characterized by IR and NMR spectroscopy, as well as by single crystal X‐ray diffraction. Due to their stability and convenient accessibility, the hydrates are ideal starting compounds for the chemistry of tris(pentafluoroethyl)gallium and ‐indium compounds, for example hydroxogallates and ‐indates. An attempt to determine its gas‐phase structure and that of the gallium analogue by electron diffraction demonstrates that they lose water ligands upon evaporation. The energetics of these processes were predicted by quantum‐chemical calculations as was the nature of bonding in the free Lewis acids and their mono‐ and dihydrates. Furthermore, the synthesis and structural diversity of hydroxido tris(pentafluoroethyl) gallates and one indate are presented.