bluuebunny/crossref_metadata_2025_split huggingface dataset:
A experiência de traduzir a novela Curial e Guelfa (séc. XV) para a língua portuguesa
Authors: Da Costa Ricardo 
Published: December 2023

Resumo: Richard Fletcher (1944-2005) estava certo: nada concentra tanto a mente de um historiador em um texto quanto a tarefa de traduzi-lo. A sublime compreensão histórica (erhabenen historischen Verständnis) abarca o tênue instante em que nos deparamos com as palavras no tempo, ponderamos sua inserção cronológica sociocultural e, cuidadosamente, as conduzimos para o presente e as recolocamos fora de sua época, tentando, da melhor maneira possível, preservar seus significados temporais subjacentes. Mas como realizar esse exercício da melhor maneira? O objetivo deste artigo é apresentar nossa proposta de tradução da novela de cavalaria quatrocentista (e Humanista) Curial e Guelfa e discutir algumas de nossas encruzilhadas, nossas opções, nossas tentativas de trazer à mente do leitor a cultura do passado e a manter viva nela, e ambientar sua reconstrução histórica no melhor espaço imaginário possível, isto é, aquele que respeita os paradoxos do tempo e as multifacetadas contradições inerentes à vida de seus personagens sociais.
Palavras-chave: tradução, Curial e Guelfa, novela de cavalaria, literatura, humanismoAbstract: Richard Fletcher (1944-2005) was right: nothing concentrates as much the historian’s mind than the task of translating a text. The sublime historical understanding (erhabenen historischen Verständnis) covers the tenuous moment that we face the words in time, pondering its chronological and socio-cultural insertion, and carefully bring them to the present and reposition them out of their time, trying as best as possible, to preserve their underlying temporal meanings. But how to exercise this in the best way? The purpose of this article is to present our proposal of translation of the humanist novel of chivalry from the fifteenth century Curial e Güelfa and discuss some of our crossroads, choices, and attempts to bring to the reader’s mind the culture of the past and keep it alive in his thoughts, and acclimatize his historical reconstruction in the best imaginary space as possible, i.e., the one that respects the paradoxes of time and the multifaceted contradictions inherent to the life of their social characters.
Keywords: translation, Curial e Güelfa, chivalry novel, literature, humanism
Benchmarking long-read genome sequence alignment tools for human genomics applications
Authors: LoTempio Jonathan , Delot Emmanuele , Vilain Eric 
Published: December 2023

Background
The utility of long-read genome sequencing platforms has been shown in many fields including whole genome assembly, metagenomics, and amplicon sequencing. Less clear is the applicability of long reads to reference-guided human genomics, which is the foundation of genomic medicine. Here, we benchmark available platform-agnostic alignment tools on datasets from nanopore and single-molecule real-time platforms to understand their suitability in producing a genome representation.

Results
For this study, we leveraged publicly-available data from sample NA12878 generated on Oxford Nanopore and sample NA24385 on Pacific Biosciences platforms. We employed state of the art sequence alignment tools including GraphMap2, long-read aligner (LRA), Minimap2, CoNvex Gap-cost alignMents for Long Reads (NGMLR), and Winnowmap2. Minimap2 and Winnowmap2 were computationally lightweight enough for use at scale, while GraphMap2 was not. NGMLR took a long time and required many resources, but produced alignments each time. LRA was fast, but only worked on Pacific Biosciences data. Each tool widely disagreed on which reads to leave unaligned, affecting the end genome coverage and the number of discoverable breakpoints. No alignment tool independently resolved all large structural variants (1,001–100,000 base pairs) present in the Database of Genome Variants (DGV) for sample NA12878 or the truthset for NA24385.

Conclusions
These results suggest a combined approach is needed for LRS alignments for human genomics. Specifically, leveraging alignments from three tools will be more effective in generating a complete picture of genomic variability. It should be best practice to use an analysis pipeline that generates alignments with both Minimap2 and Winnowmap2 as they are lightweight and yield different views of the genome. Depending on the question at hand, the data available, and the time constraints, NGMLR and LRA are good options for a third tool. If computational resources and time are not a factor for a given case or experiment, NGMLR will provide another view, and another chance to resolve a case. LRA, while fast, did not work on the nanopore data for our cluster, but PacBio results were promising in that those computations completed faster than Minimap2. Due to its significant burden on computational resources and slow run time, Graphmap2 is not an ideal tool for exploration of a whole human genome generated on a long-read sequencing platform.
Differentially expressed genes in orbital adipose/connective tissue of thyroid-associated orbitopathy
Authors: Wang Yan , Liu Yanqiu , Cai Jiping , Zong Tianyi , Zhang Ziyin , Xie Tianhua , Mu Tong , Wu Meili , Yang Qian , Wang Yangningzhi , Wang Xiaolu , Yao Yong 
Published: December 2023

Background
Thyroid-associated orbitopathy (TAO) is a disease associated with autoimmune thyroid disorders and it can lead to proptosis, diplopia, and vision-threatening compressive optic neuropathy. To comprehensively understand the molecular mechanisms underlying orbital adipogenesis in TAO, we characterize the intrinsic molecular properties of orbital adipose/connective tissue from patients with TAO and control individuals.

Methods
RNA sequencing analysis (RNA-seq) was performed to measure the gene expression of orbital adipose/connective tissues of TAO patients. Differentially expressed genes (DEGs) were detected and analyzed through Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and Gene Set Enrichment Analysis (GSEA). The protein–protein interaction (PPI) network was constructed using the STRING database, and hub genes were identified by the Cytoscape plug-in, cytoHubba. We validated several top DEGs through quantitative real-time polymerase chain reaction (qRT–PCR).

Results
We identified 183 DEGs in adipose tissue between TAO patients (n = 3) and control patients (n = 3) through RNA sequencing, including 114 upregulated genes and 69 downregulated genes. The PPI network of these DEGs had 202 nodes and 743 edges. PCR-based validation results of orbital adipose tissue showed multiple top-ranked genes in TAO patients (n = 4) are immune and inflammatory response genes compared with the control individual (n = 4). They include ceruloplasmin isoform x3 (CP), alkaline tissue-nonspecific isozyme isoform x1 (ALPL), and angiotensinogen (AGT), which were overrepresented by 2.27- to 6.40-fold. Meanwhile, protein mab-21-like 1 (MAB21L1), phosphoinositide 3-kinase gamma-subunit (PIK3C2G), and clavesin-2 (CLVS2) decreased by 2.6% to 32.8%. R-spondin 1 (RSPO1), which is related to oogonia differentiation and developmental angiogenesis, was significantly downregulated in the orbital muscle tissues of patients with TAO compared with the control groups (P = 0.024).

Conclusions
Our results suggest that there are genetic differences in orbital adipose-connective tissues derived from TAO patients. The upregulation of the inflammatory response in orbital fat of TAO may be consistent with the clinical phenotype like eyelid edema, exophthalmos, and excess tearing. Downregulation of MAB21L1, PIK3C2G, and CLVS2 in TAO tissue demonstrates dysregulation of differentiation, oxidative stress, and developmental pathways.
Socioeconomic Impact of Agricultural Projects Financed by the District Development Fund: Case Study of the District of Vilankulo - Mozambique
Authors: Matsinhe Graciano , Chimene Catine 
Published: December 2023

According to the Constitution of the Republic of Mozambique, agriculture is recognized as the cornerstone of the country's development. However, the levels of production and productivity in this sector remain low. In response to this challenge, the Government established and implemented the District Development Fund (DDF) to provide financial support for economic activities, with a particular focus on agriculture (IBIS, 2010). Therefore, the goal of this research was to analyze the socioeconomic impact of the DDF on the beneficiary communities. To achieve this objective, semi-structured interviews of 36 beneficiaries of DDF and unsystematic observations were conducted. The results indicated that all participants reported a significant increase, six times higher than before, in their agricultural production areas. This enhancement allowed for the expansion and diversification of agricultural products. The surplus generated from the sale of these products was used for personal purposes, such as purchasing radios, telephones, school supplies for their children, and improving their housing conditions. Consequently, these improvements significantly enhanced the quality of life for those involved in agriculture. These findings strongly align with the objectives underlying the creation of the DDF and could serve as an exemplar for other districts within the country. In conclusion, it can be affirmed that the DDF has made significant contributions to improving the living conditions of the beneficiary population in the district of Vilankulo.
Impact of Submergence1 (Sub1) Locus in Genetic Background of Modern Rice Varieties (Oryza sativa L.)
Authors: R. Salomi , P. Vignesh , S. Bharathkumar 
Published: December 2023

Aim: To improve submergence stress tolerance of modern rice varieties (ADT36 and ADT37) through selection of superior rice lines.
Study Design: Backcross method.
Place and Duration of Study: This effort was taken in Nethouse of Kandaswami Kandar’s College, Namakkal District, Tamil Nadu, India. This process was completed in four years from 2019 to 2022.
Methodology: Here, widely cultivated two short-term rice varieties (ADT36 and ADT37) were selected to improve submergence tolerance based on polymorphism at phenotypic and genotypic level. Then, they were improved through backcrossing with respective recurrent parent by selection of superior rice lines compared to parental lines under submergence condition. Likewise, this process was advanced up to BC3F2 generation.
Results: In this study, a number of two NILs for ADT36 rice variety (ADT36- F12-5-19.18 and ADT36- F15-2-5.17) and five NILs for ADT37 rice variety (ADT37- F113-27-5.2, ADT37- F113-27-5.5, ADT37- F115-2-1.10, ADT37- F115-2-1.14 and ADT37- F115-2-2.14) are selected as superior lines.Selected rice lines exhibited more than 60% submergence tolerance than donor parent. This result indicates the adaptation of Sub1 locus into new genetic background of ADT36 and ADT37 rice variety under submergence condition.
Conclusion: Improvement process of rice lines under stress condition results in identification of highly active rice lines. Thus, selected submergence tolerant rice lines would be a boon to the rice farmers who are facing crop lose due to frequently occurring cyclones in the Cauvery delta region.
Effect of Different Sources of Nitrogen and Phosphorus and Application Methods on Yield and Quality of Hybrid Pearl Millet
Authors: Morya Rahul , Trivedi S. K. , Yadav Shashi S. , Gupta Naresh 
Published: December 2023

Due to high yielding and short duration hybrid pearl millet got populized in last few years under pearlmillet-mustard/wheat cropping system but its required higher nutrient as compared to composite varieties but farmers apply only N and little amount of P which is responsile for low yield therefore, the present study was carried out during kharif 2021 and 2022 with 12 treatments of different sources which are chemical fertilizers, vermicompost (VC) and bio-fertilizers(Azotobacter and PSB) and application methods (seed treatment, soil and foliar application) of nitrogen and phosphorus in RBD with 3 replications. The resulted indicated that the application of 100% Nitrogen and Phosphorus by chemical fertilizers produced significantly higher yield attributes (length and girth of cobs) and yield parameter (grain and stover yield and harvest index) of pearlmilletas compared to 50 and 75% NP treatments. Maximum grain yield 29.87 q ha-1) was noted with integration of chemical, organic and bio fertilizers (75% NP + Seed treatment by Azoto + PSB + 5 t VCha-1) which was significantly higher over rest of all other treatments. Minimum yield parameters were noted with control (0 % NP). Application of vermicompost @ 5 t ha-1 with 75 per cent of NP and inoculation of Azotobacter and PSB improved test weight and protein content.
An Examination of Physical Activity, Non-Communicable Diseases, and Health Screening among People Living with HIV in Zamfara State, Nigeria
Authors: Zurmi Nasiru Bala , Magaji Bello Arkilla , Luximon-Ramma Amitabye 
Published: December 2023

Aim: This study provides a comprehensive analysis of physical activity levels, non-communicable disease prevalence, and health screening practices of people living with HIV in Zamfara State, Nigeria.
Methodology: This study is a cross-sectional study involving a total of 424 individuals living with HIV (PLWH) in Zamfara State, Nigeria.
Results: The socio-demographic analysis reveals that the study participants were predominantly within the age group of 30-39 (38.7%) and were mainly female (70.3%). In terms of ethnicity, Hausa was the most common (75.7%), followed by Yoruba (9.7%) and Igbo (4.7%). The participants were largely married (57.8%) and had no formal schooling (45.8%). Physical activity levels were evaluated based on the time spent on different domains of activity in a typical week. The mean time spent on vigorous intensity activity was 28.6 minutes per week, while moderate-intensity activity and walking/cycling were higher at 69.9 minutes and 205.1 minutes per day, respectively. However, 47.2% of participants overall did not meet WHO recommendations on physical activity for health, with females demonstrating a higher rate of non-compliance (50.7%) compared to males (38.9%). A notable aspect of the study is the examination of the prevalence of non-communicable diseases (NCDs) among the participants. Overall, the highest prevalence was seen for raised blood pressure (10.4%), followed by cardiovascular diseases (7.3%) and raised blood sugar (4.0%), while raised cholesterol levels were minimal (0.2%). Females presented a higher prevalence of raised blood pressure and cardiovascular diseases. The study also scrutinized health screening practices, revealing that the screening for cervical cancer among female respondents was worryingly low at 2.3%. The mean weight and height for both sexes were 63.8 Kg and 1.6 meters, respectively. The mean Body Mass Index (BMI) was found to be 23.5 for males and 25.5 for females. Concerningly, 37.5% of the population had a BMI greater than 25, indicating overweight or obesity, with a higher prevalence among females (31.8%) than males (7.6%).
Conclusion: This study provides valuable insights into physical activity trends, non-communicable disease prevalence, and health screening practices among PLWH in Zamfara State, Nigeria. The findings underline the importance of tailored interventions to promote physical activity, manage non-communicable diseases, and encourage routine health screenings in this demographic.
Use of New Audio-Only Telemedicine Claim Modifiers
Authors: Morenz Anna M. , Staloff Jonathan , Liao Joshua M. , Wong Edwin S. 
Published: December 2023

ImportancePolicymakers at both the state and federal levels face decisions about coverage of audio-only telemedicine amid a dearth of reliable data due to changes and variation in billing practices.ObjectiveTo describe early trends in the use of new audio-only telemedicine claims modifiers 93 and FQ in Washington State, which were introduced to improve the designation and identification of audio-only telemedicine claims.Design, Setting, and ParticipantsThis retrospective cohort study analyzed claims data from the Washington All-Payer Claims Database from January to November 2022. Participants included 4.3 million children and adults insured for at least 6 months in 2021 through public or private insurance plans.ExposuresUse of audio-only telemedicine was compared by age, race, ethnicity, insurance type, rurality, and Social Vulnerability Index.Main Outcomes and MeasuresAudio-only telemedicine services were identified by claims appended by Current Procedural Terminology (CPT) code modifiers 93 or FQ or that included telephone-only CPT codes. Modifiers 93 and FQ denote audio-only telemedicine services for any reason and for behavioral health concerns, respectively.ResultsIn 2022, there were a total of 917 589 audio-only telemedicine services, of which 345 941 (38%) were appended with modifier FQ and 55 352 (6%) with modifier 93. Audio-only telemedicine services with these modifiers were most frequent for behavioral health diagnoses or routine prenatal and postpartum care. Individuals who used telemedicine exclusively via audio-only modality were more likely to be older (mean [SD] age, 46.0 [22.5] vs 42.0 [21.4] years) and insured by Medicare (41 758 of 196 225 [21%] vs 95 962 of 707 626 [14%]) than those who used at least 1 audiovisual service.Conclusions and RelevanceIn this cohort study of a statewide all-payer claims database, modifiers 93 and FQ offered the important capability to identify audio-only telemedicine services beyond telephone-only CPT codes, but their uptake remained low. Audio-only telemedicine appears to offer an important means for access to behavioral health and perinatal care access, but further work is needed to study outcomes and quality of care.
Risk‐based scheduling of CCHP‐based microgrid considering economic and environmental aspects using confidence degree theory and CVaR approach
Authors: Ran Liang , Yu Jian , Ma Zhiwen , Liu Caiyan 
Published: December 2023

AbstractThe combined cooling, heating, and power (CCHP) co‐generation system is an alternative for developing sustainable energy systems. Inside a multi‐energy CCHP microgrid, electric, heat, and cool demands are supplied with a high efficiency. Integrating various energy conversion technologies and storage systems allows managing different resources and taking advantage of electric market participation. However, the uncertainties associated with source demand and prices should be taken into account. In this regard, this paper proposes stochastic programming to optimize the operation cost and emission penalty of a multi‐energy CCHP microgrid considering the mathematical model of components and related uncertainties. Using the proposed optimization problem, the system operator can derive bidding/offering curves in the electric market. To mitigate the financial risks, the conditional value‐at‐risk (CVaR) approach is integrated to provide different risk‐averse strategies. From the results, it is found that under the risk‐averse strategy, by paying 0.4% more money, the risk of CCHP's operation cost instability will be reduced by approximately 16.82%.
Biomimetic Hydrogels as the Inductive Endochondral Ossification Template for Promoting Bone Regeneration
Authors: Zhao Fujian , Qiu Yonghao , Liu Wenjing , Zhang Yanli , Liu Jia , Bian Liming , Shao Longquan 
Published: December 2023

AbstractRepairing critical size bone defects (CSBD) is a major clinical challenge and requires effective intervention by biomaterial scaffolds. Inspired by the fact that the cartilaginous template‐based endochondral ossification (ECO) process is crucial to bone healing and development, developing biomimetic biomaterials to promote ECO is recognized as a promising approach for repairing CSBD. With the unique highly hydrated 3D polymeric network, hydrogels can be designed to closely emulate the physiochemical properties of cartilage matrix to facilitate ECO. In this review, the various preparation methods of hydrogels possessing the specific physiochemical properties required for promoting ECO are introduced. The materiobiological impacts of the physicochemical properties of hydrogels, such as mechanical properties, topographical structures and chemical compositions on ECO, and the associated molecular mechanisms related to the BMP, Wnt, TGF‐β, HIF‐1α, FGF, and RhoA signaling pathways are further summarized. This review provides a detailed coverage on the materiobiological insights required for the design and preparation of hydrogel‐based biomaterials to facilitate bone regeneration.
Point‐Of‐Care Ultra‐Portable Single‐Molecule Bioassays for One‐Health
Authors: Macchia Eleonora , Torricelli Fabrizio , Caputo Mariapia , Sarcina Lucia , Scandurra Cecilia , Bollella Paolo , Catacchio Michele , Piscitelli Matteo , Di Franco Cinzia , Scamarcio Gaetano , Torsi Luisa 
Published: December 2023

AbstractScreening asymptomatic organisms (humans, animals, plants) with a high‐diagnostic accuracy using point‐of‐care‐testing (POCT) technologies, though still visionary holds great potential. Convenient surveillance requires easy‐to‐use, cost‐effective, ultra‐portable but highly reliable, in‐vitro‐diagnostic devices that are ready for use wherever they are needed. Currently, there are not yet such devices available on the market, but there are a couple more promising technologies developed at readiness‐level 5: the Clustered‐Regularly‐Interspaced‐Short‐Palindromic‐Repeats (CRISPR) lateral‐flow‐strip tests and the Single‐Molecule‐with‐a‐large‐Transistor (SiMoT) bioelectronic palmar devices. They both hold key features delineated by the World‐Health‐Organization for POCT systems and an occurrence of false‐positive and false‐negative errors <1–5% resulting in diagnostic‐selectivity and sensitivity >95–99%, while limit‐of‐detections are of few markers. CRISPR‐strip is a molecular assay that, can detect down to few copies of DNA/RNA markers in blood while SiMoT immunometric and molecular test can detect down to a single oligonucleotide, protein marker, or pathogens in 0.1mL of blood, saliva, and olive‐sap. These technologies can prospectively enable the systematic and reliable surveillance of asymptomatic ones prior to worsening/proliferation of illnesses allowing for timely diagnosis and swift prognosis. This could establish a proactive healthcare ecosystem that results in effective treatments for all living organisms generating diffuse and well‐being at efficient costs.