bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is proanthocyanidin associated to vitamin E or palm oil able to protect enamel against initial erosion in vitro?
Authors: Martins Daiana da Silva , Boteon Ana Paula , Silva Thayná Theodoro da , Sabino Julia Fiorese , Ionta Franciny Querobim , de Oliveira Angélica Aparecida , Honório Heitor Marques , Buzalaf Marília Afonso Rabelo , Carvalho Thiago Saads , Rios Daniela 
Published: December 2023

Abstract
Objectives
This in vitro study evaluated the effect of Proanthocyanidin, Palm Oil and Vitamin E against initial erosion.
Materials and Methods
Bovine enamel blocks (n = 140) were divided into 14 groups: PC_SnCl2/NaF/Am-F-containing solution (positive control); NC_deionized water (negative control); PO_palm oil; P6.5_6.5% proanthocyanidin; P2_2% proanthocyanidin; VE_Vitamin E; POP6.5_palm oil + 6.5% proanthocyanidin; P6.5PO_6.5% proanthocyanidin + palm oil; POP2_palm oil + 2% proanthocyanidin; P2PO_2% proanthocyanidin + palm oil; VEP6.5_Vitamin E + 6.5% proanthocyanidin; P6.5VE_6.5% proanthocyanidin + Vitamin E; VEP2_Vitamin E + 2% proanthocyanidin; P2VE_2% proanthocyanidin + Vitamin E. The acquired enamel pellicle (AEP) was previously formed in situ for 30 min. The specimens were treated in vitro with the solutions (500 µl, 30s). Then, the blocks were maintained for an additional hour in oral cavity to develop the modified AEP. The blocks were immersed in 0.5% citric acid (pH 2.5) during 30s. The response variable was the percentage of surface hardness loss. Data were analyzed by one-way ANOVA and Fisher's Test (p < 0.05).
Results
P6.5VE was the only group to promote protection similar to PC. PO, P2, POP6.5, P2PO, P6.5VE and P2VE exhibited %SHL similar to both PC and NC groups. (p < 0.05). P6.5, VE, P6.5PO, POP2, VEP6.5 and VEP2 were different to PC and similar to NC.
Conclusion
It is concluded that proanthocyanidin associated to vitamin E was able to protect enamel against in vitro initial erosive challenge.
Clinical Relevance:
This study suggests the potential for a cost-effective anti-erosion product. Further investigations across diverse experimental models are warranted to validate these promising outcomes.
The cardiovascular benefits of glucagon-like peptide-1 receptor agonists as novel diabetes drugs are mediated via the suppression of miR-203a-3p and miR-429 expression
Authors: Liu Yanfen , Nie Dongying , Lou Xueyong 
Published: December 2023

Abstract
Purpose
Coronary artery disease (CAD) is associated with a high fatality rate and a heavy global health care burden. Glucagon-like peptide-1 (GLP-1) exerts positive cardiovascular effects, although the molecular mechanisms are unclear. Therefore, this study aimed to verify whether the cardioprotective effects of GLP-1 are mediated through the regulation of micro-RNA (miRNA) expression.
Materials and Methods
Follow-up assessments were conducted for 116 patients with type 2 diabetes alone (controls) and 123 patients with both type 2 diabetes and CAD. After matching, each group comprised 63 patients, and age, body mass index, and serum levels of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, and haemoglobin A1C were compared. Subsequently, the expression profiles of four circulating miRNAs (miR-203a-3p, miR-429, miR-205-5p, and miR-203b-5p) were assessed via qRT-PCR in the 63 patients with diabetes and CAD between six months (baseline) and twelve months after the initiation of GLP-1 therapy.
Results
As expected, the metabolic factors were significantly improved after 6 months of treatment with GLP-1 compared with pre-treatment values, and the expression levels of two of the miRNAs (miR-203a-3p and miR-429) decreased from baseline levels in those with diabetes and CAD.
Conclusions
The results suggest that the cardiovascular benefits induced by GLP-1 are mediated via suppressed expression of two miRNAs: miR-203a-3p and miR-429.
Determination of charring conditions of archaeological grape seeds, towards machine-learning based classification
Authors: Reuveni Yuval , Landa Vlad , Shapira Yekaterina , Behar Adi , Ben-Arie Reut , Weiss Ehud , Drori Elyashiv 
Published: December 2023

Abstract
This study investigates the chemical and morphological changes in grape pips resulting from various charring conditions. Employing Fourier Transform Infrared Spectroscopy (FTIR) for chemical analysis and high-resolution scanning combined with morphometric measurements for morphological analysis, we aimed to understand the effects of charring on grape pips. Our hypothesis regarding the potential of chemical composition for distinguishing charring temperatures was partially supported, as FTIR analysis revealed distinctive changes in chemical bonds at different temperatures. However, FTIR spectra of archaeological seeds showed limited utility for identification due to postdeposition alterations masking the original chemical fingerprint. In addition, morphometric analysis demonstrated significant alterations in seed shape above 250℃, corroborating FTIR findings. The length:width ratio and the occurrence of cracks notably changed, providing a basis for assessing charring conditions. Applying a machine learning classification method, we determined that accurate classification of grape varieties by the morphometric analysis method is feasible for seeds charred at up to 250℃ and 8 hours. Integrating the morphometric changes and temperature ranges suitable for classification, we developed a sorting model for archaeological seeds. By projecting length:width ratios onto a curve calculated from controlled conditions, we estimated charring temperatures. Approximately 50% of archaeological seeds deviated from the model, indicating drastic charring conditions. This sorting model facilitates a stringent selection of seeds fit for classification, enhancing the accuracy of our machine learning-based methodology. In conclusion, combining machine learning with morphometric sorting enables the identification of charred grape seeds suitable for identification by the morphometric method. This comprehensive approach provides a valuable tool for future research for the identification of charred grape seeds found in archaeological contexts, enhancing our understanding of ancient viticulture practices and grape cultivation.
Dexamethasone treatment influences tendon healing through altered resolution and a direct effect on tendon cells
Authors: Dietrich-Zagonel Franciele , Alim MD Abdul , Beckman Leo Bon , Eliasson Pernilla 
Published: December 2023

Abstract
Inflammation, corticosteroids, and loading all affect tendon healing, with an interaction between them. However, underlying mechanisms behind the effect of corticosteroids and the interaction with loading remain unclear. The aim of this study was to investigate the role of dexamethasone during tendon healing, including specific effects on tendon cells.
Rats (n = 36) were randomized to strong or moderate loading, the Achilles tendon was transected, and animals were treated with dexamethasone or saline. Gene and protein analyses of the healing tendon were performed for extracellular matrix-, inflammation-, and tendon cell markers. We further tested specific effects of dexamethasone on tendon cells in vitro.
Dexamethasone increased mRNA levels of S100A4 and decreased levels of ACTA2/α-SMA, irrespective of load level. Strong loading + dexamethasone reduced mRNA levels of FN1 and TenC (p < 0.05), while resolution-related genes were unaltered (p > 0.05). In contrast, moderate loading + dexamethasone increased mRNA levels of resolution-related genes ANXA1, MRC1, PDPN, and PTGES (p < 0.03). Altered protein levels were confirmed in moderately loaded tendons. Dexamethasone treatment in vitro prevented tendon construct formation, increased mRNA levels of S100A4 and decreased levels of SCX and collagens.
Dexamethasone during tendon healing appear to act through immunomodulation by promoting resolution, but also through a direct effect on tendon cells.
On the Generalized Jordan-Derivation Over -Paranormal Operators
Authors: Hamada Nuha 
Published: December 2023

Abstract
Let B(H) be the set of all bounded linear operations on the separable infinite dimensional Hilbert spaces. The mapping $J_{A, B}(T)=TA-BT^*$ is called generalized Jordan*-derivations. Researchers have thoroughly investigated the characteristics of $J_{A, B}(T)$ within specific classes of operators A and B. The characterization of the properties of A and B relies significantly on the range of $J_{A, B}(T)$. In this paper, we investigate the generalized Jordan *-derivations $J_{A, B}(T)=TA-BT^*$ over *- paranormal operators A and B. The author shows among other results that if the identity operator belongs to the range of generalized Jordan *-derivations $J_{A, B}$ over *- paranormal operators A and B where $\partial\sigma(A)=\partial\sigma(B)$, then A and B are invertible skew-Hermitian operators.
An Exploratory Study of the Experiences of African International Students in U.S. Higher Education
Authors: Amoateng Ernest Yaw 
Published: December 2023

Abstract
Given the increasing number of international students attending US higher education institutions, it is imperative to study the experiences of African students’ transition period, as they acculturate into the American culture and educational system. The uniqueness of African international students may be different from the experiences of international students from other regions. As a result of the account of African students, university counseling centers, campus services, and those who interact with and support these students, the initial and ongoing stress of living in a foreign country and adapting to a new way of life can be eased. Six African international students participated in individual semi-structured qualitative interviews conducted at a Midwest research university. To better understand cross-cultural adjustment, a basic interpretive qualitative research (BIQR) design was utilized. The interview texts were analyzed based on the basic interpretive qualitative approach, which has been described as containing elements of induction, theoretical, and constant comparison. Findings indicated specific experiences regarding African international students’ adjustment to the US environment, including strategies perceived as helpful in specific situations. Based on the analysis of participant interviews, the major themes that arose from the interviews included: academic prowess; social integration; and cultural integration. Implications for research and practice have been discussed. It is recommended that future researchers examine the unique subpopulations of international students’ cross-cultural adjustments and experiences.
Sulphotransferase (SOT) genes in wheat providing tolerance against drought and heat
Authors: chaudhary Jyoti , Gangwar Himanshi , Jaiswal Vandana , Gupta Pushpendra Kumar 
Published: December 2023

Abstract
Sulphotransferase (SOT) genes that are involved in sulphonation during sulphate metabolism, represent a conserved family of genes, which provide tolerance against abiotic stresses like heat and drought in plants. In plants, sulphate metabolism is a component of primary metabolism, where SOTs catalyze sulphonation. In the present study in wheat, 107 TaSOT genes were identified; 78 of these genes were identified through genome-wide search and the remaining 29 identified through SOT domain (PF00685) (sulfotransferase family protein). All 107 genes were mapped onto individual chromosomes. The length of these ranged from 969bp to 7636bp; 73 (68%) of these 107 genes are intronless. The gene sequences were associated with 48 different cis regulatory elements. The genes also carry some tandem/segmental duplications, SSRs (simple sequence repeats), target sites for miRNAs and sequences for some lncRNAs. The promoters of some genes also carried transcription factor binding sites (TFBS). The SOT proteins carried two highly conserved 5’-phosphosulphate-binding (5’ PSB) regions and a 3’-phosphate-binding (3’ PB) motif that are essential for SOT activities. Homology modeling of TaSOT proteins and docking analysis of their ligand-binding sites revealed the presence of important amino acids in their active sites. An expression assay of TaSOT genes via wheat RNA-seq data suggested engagement of these genes in growth, development and responses to various hormones and biotic/abiotic stresses. The results of the present study should help in further functional characterization of SOT genes in wheat and other related crops.
An efficient monitoring of HELLP syndrome pre-eclampsia in wireless sensors networks
Authors: ullah Muneeb , Young Xiadong , khan Muhammad Faizan , Junaid Junaid , Dai Shihan 
Published: December 2023

Abstract
This paper explores the application of wireless sensing using 5G technology in the 4.8 GHz C-band, a significant step forward in healthcare innovation. It focuses on the application of wireless sensing to monitor HELLP syndrome in cases of pre-eclampsia, showcasing how Wireless Sensor Networks (WSNs), enhanced by 5G's high-speed capabilities, substantially improve real-time data transmission and healthcare decision-making. The integration of WSNs with 5G technology enables non-invasive, continuous patient monitoring, providing advanced solutions for remote health surveillance and efficient data management in critical healthcare situations. Specifically, the study highlights the use of a wireless transceiver in indoor environments to monitor various body movements, including those indicative of HELLP syndrome symptoms. These movements generate unique wireless data, thus enriching the understanding of wireless channel information. The research explores deep learning models such as ANN, CNN, and especially VGGNet, which achieved a notable 99.26% accuracy in classifying patient activities. Additionally, the paper discusses model optimization, emphasizing the need for adjustments in parameters such as batch sizes and hidden units to enhance performance. The study's outcomes, evaluated using metrics such as accuracy, recall, precision, specificity, and F-measure, demonstrate the superior performance of VGGNet compared to other classifiers. These findings underscore the potential of integrating advanced technologies like WSNs and 5G in healthcare, highlighting their role in creating more effective, reliable, and patient-centric healthcare systems
IN SILICO STUDY OF THE SYNERGISTIC INTERACTION OF 5-FLUOROURACIL AND CURCUMIN ANALOGUES AS INHIBITORS OF B-CELL LYMPHOMA 2 PROTEIN
Authors: AMAN LA ODE , ARFAN  , ASNAWI AIYI 
Published: December 2023

Objective: The research objective is to explore the potential for synergistic inhibition of two curcumin analogs combined with 5-fluorouracil (5-FU) against the B-cell lymphoma 2 (BCL-2) protein.
Methods: We investigated the synergistic inhibition of two curcumin analogs, namely, (1E,4E)-1,5-bis(4-hydroxyphenyl)penta-1,4-dien-3-one (AC01) and (1E,4E)-1,5-bis(3,4-dihydroxyphenyl)penta-1,4-dien-3-one (AC02), each combined with 5-FU by calculating their binding free energies and binding stability. An in silico investigation of the synergistic interaction of ligand pairs was conducted using the multiple ligand simultaneous docking (MLSD) technique with the AutoDock Vina package. The stability of interactions and binding free energies of each BCL-2 and curcumin analogs were examined by applying molecular dynamics techniques with the Gromacs package and MMPBSA method.
Results: All ligand pairs had displayed strong binding affinity, as evidenced by highly negative free energy values, indicating a robust association with BCL-2. Molecular dynamics simulations were conducted over 100 ns, confirming good stability with controlled RMSD changes, suggesting that the ligand pairs had remained securely bound to the BCL-2 binding site. Additionally, RMSF analysis and energy decomposition had revealed that ligand interactions did not influence protein residue fluctuations during the simulation, and the protein-ligand complexes had maintained stability throughout the simulation. Furthermore, binding free energy calculations using the MMPBSA method had consistently shown negative values, signifying stable interactions with BCL-2 for all ligand pairs.
Conclusion: In conclusion, our study revealed that AC01 and AC02, when combined with 5-FU, had the ability to intercalate into the P2 and P4 sites of BCL-2. This suggested that AC01 and AC02 held promise for further study as candidates for anticancer drugs, individually or in combination with 5-FU.
METHOTREXATE-INDUCED ORAL ERYTHEMA MULTIFORME
Authors: SANTOSO ASTRID WIDHOWATY , SHAFURIA AMIRA , DEWI TENNY SETIANI 
Published: December 2023

Objective: To discuss the management of oral erythema multiforme (EM) due to methotrexate (MTX) in gestational trophoblastic tumor patients.
Methods: A 28-year-old woman was referred to the Oral Medicine Clinic with complaints of pain throughout the oral cavity accompanied by blackish encrustation on the upper and lower lip for 5 d. She previously underwent the first cycle of chemotherapy using MTX for the treatment of a gestational trophoblastic tumor. Extraoral, multiple diffuse blackish patches on the face and haemorrhagic crusts on the lips with painful erosions. Intraoral, multiple erosive and ulcerative lesions on the labial and buccal mucosa.
Results: Management involved topical 0.025% hyaluronic acid mouthwash for erosion and ulceration, compressed 0.9% NaCl for crusted lesions on the lips, and petroleum jelly for the non-crusted lesions on the lips. Complete healing of oral and lip lesions was achieved within 10 d.
Conclusion: Pharmacological management should be tailored to each patient with careful consideration of treatment risk or benefit. In our case, the use of non-steroidal anti-inflammatory topical agents was considered successful in treating oral EM.
ISOLATION OF CELLULOSE FROM SIWALAN FIBER (BORASSUS FLABELLIFER L.) USING RESPONSE SURFACE METHODOLOGY
Authors: AZAMI YUSUF IKROM NUR , MUSFIROH IDA , MUCHTARIDI  , PRATIW RIMADANI , PUTRA OKTA NAMA 
Published: December 2023

Objective: This study investigated the isolation of α-cellulose powder from siwalan fibers (Borassus flabellifer L.).
Methods: The methods include delignification using 10% NaOH, and bleaching using 10% NaOCl. The present research used response surface methodology (RSM) to investigate the impact of temperature and duration on the bleaching process. Two factors were evaluated and optimized using the central composite design (CCD).
Results: The findings revealed that the hit quality and white degree were 91.5% and 52.58% when the temperature and time of bleaching were 40 °C and 30 min, respectively. Utilizing an analysis of variance (ANOVA), the most influential factors on the percentage of strike quality and whiteness degree were identified. Absorption at 3323.34 cm-1 for O-H, 2893.22 cm-1 for C-H, 1371.38 cm-1 for C-O-H, and 1157.28 cm-1 for C-O, according to FTIR characterization results, are characteristic cellulose peaks.
Conclusion: The cellulose characterization results acquired in this work show that the temperature in the bleaching process has a significant effect on the cellulose isolation results.
EFFICACY OF CHLORINE DIOXIDE ON ORAL LESIONS IN ACUTE MYELOBLASTIC LEUKEMIA PATIENTS UNDERGOING CHEMOTHERAPY
Authors: RIDHO FAIZNUR , FITRIASARI NURI , ZAKIAWATI DEWI 
Published: December 2023

Objective: The aim of this study was to describe the pharmacological management of oral lesions, particularly using chlorine dioxide agents in an acute myeloblastic leukemia (AML) patient undergoing chemotherapy.
Methods: A 9 y old girl was referred from the Department of Paediatrics at Hasan Sadikin Hospital to the Department of Oral Medicine with complaints of swelling on the lips, difficulty while eating, swallowing, and even opening the mouth. Extra oral examination showed angioedema on the lips with a prominent serosanguinolent crust. Intraoral examination revealed white plaques that could not be scrapped off in all parts of the mouth. The patient was diagnosed with AML and had received chemotherapy until the second cycle.
Results: Gauze soaked in chlorine dioxide oral rinse was applied to compress the patient's lips. Following this, 0.2% hyaluronic acid gel was also administered. In terms of intraoral treatment, chlorine dioxide was also applied by spraying it throughout the entire mouth. After one month, the lesion had completely healed, allowing the patient to open their mouth without any issues.
Conclusion: Chlorine dioxide significantly improves the oral mucosal lesions in an AML patient undergoing chemotherapy.
LOZENGES FORMULATION OF CIPLUKAN (PHYSALIS ANGULATA L.) FRUIT EXTRACT AS AN ANTIOXIDANT WITH COMBINATION OF FILLER AGENTS AVICEL PH 102–LUDIPRESS
Authors: TANJUNG YENNI PUSPITA , AKMAL TUBAGUS , JULIANTI ANDI IKA , FAUZIYYAH AMANINA , RIKI APEP 
Published: December 2023

Objective: This research aimed to determine the effect of variations in the concentration of filler agents Avicel PH 102-Ludipress on the evaluation results of granules and tablets and to determine the antioxidant activity of ciplukan (Physalis angulata L.) fruit extract lozenges.
Methods: The extract was obtained by the soxhletation method using ethanol 96%. The lozenges were made by direct compression, and the formula was divided into 3 concentration variations of Avicel PH 102 and Ludipress, namely F1 (1:2), F2 (1:1), and F3 (2:1). The best lozenges were tested for their antioxidant activity using the DPPH method.
Results: The results of statistical analysis using the One Way Anova test formulation of ciplukan fruit extract lozenges with variations in concentrations of Avicel PH 102 and Ludipress had a significant effect (p<0.05) on the angle of repose, bulk density, tapped density, Carr's index, Hausner's ratio, thickness, friability, and disintegration time, but no significant effect (p>0.05) on diameter, weight variation, and hardness. The best ciplukan fruit extract (F1) lozenge formula has a concentration ratio of Avicel PH 102 and Ludipress (1:2) with an IC50 value of 28.46 ppm and is classified as a very strong antioxidant.
Conclusion: Ciplukan fruit extract formulated in the form of lozenges with varying concentrations of the filler Avicel PH 102: Ludipress (1:2) has very strong antioxidant activity.
OPTIMIZATION OF LACTOBACILLUS PLANTARUM FERMENTATION FOR ENHANCED PHENOLIC PRODUCTION FROM EXTRACT OF BAJAKAH BARK (UNCARIA NERVOSA) BY RESPONSE SURFACE METHODOLOGY
Authors: ALMEIDA MARIA , NARSA ANGGA CIPTA , KUSUMA SRI AGUNG FITRI , SRIWIDODO SRIWIDODO 
Published: December 2023

Objective: This research was purposed to optimize the fermentation medium and condition of Lactobacillus plantarum to enhance the production of phenolic compounds with the antibacterial activity tested against Propionibacterium acnes and the optimum conditions of bajakah bark fermentation using Response Surface Method (RSM) methods.
Methods: The antibacterial activity test ethanolic extract of bajakah bark was assayed using the agar diffusion perforator method and fermentation method using L. plantarum. Measurement of phenolic content fermentation and without fermentation was done using the standard methods with gallic acid as standard. The optimum conditions of bajakah bark fermentation using RSM methods using Software Design Expert 10.0.0.1.
Results: As the result, Response surface analysis revealed the optimum values of the tested significant variables for the production of phenolic were extracted in a concentration 40% (w/v) and 30% (w/v) sucrose for 3 d fermentation period. Under this optimal condition, the phenolic compounds were improved from 10.66 to 18.46 mg GAE/g extract. As well as the antibacterial activity of the fermented extract was increased by 1.35 times compared to the non-fermented extract.
Conclusion: In summary that, the optimized fermentation condition could be helpful for the production of antibacterial metabolites from bajakah bark by L. plantarum.