bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Turn‐On Fluorescence Probe for Rapidly Sensing Exogenous and Endogenous Hydrogen Peroxide in Living Cells
Authors: Ding Zixuan , He Xianlin , Huang Jianan , Zhang Ruqi , Song Yanting , Yang Haimei 
Published: February 2025

AbstractMonitoring abnormal changes in hydrogen peroxide (H2O2) levels in mammalian cells is essential to understanding its physicochemical functions in biological systems. Generally, H2O2 fluorescent probes used for intracellular monitoring are limited to the long response time and low sensitivity. Herein, the 1,8‐naphthimide‐based fluorescent probe (NPB) with excellent photostability was confirmed to be an H2O2 specific probe due to the oxidation of borate ester to phenol. Compared with previously reported molecular probes, NPB displayed improved performance in H2O2 detection, such as excellent photostability, good anti‐interference, high selectivity, fast response (10 s), and low detection limit (15 nM). Furthermore, the most outstanding advantage of the as‐prepared NPB was mitochondrial‐targeting ability with a Pearson's colocalization coefficient of 0.91. Meanwhile, the probe NPB with excellent biocompatibility was successfully utilized for imaging exogenous and endogenous H2O2 in living cells. The sensing mechanism of NPB to H2O2 was further carefully demonstrated and proposed to involve the oxidation of borate to phenol by H₂O₂. We anticipated that the as‐prepared NPB should have broad application in chemical analysis and reveal the physiological function of H2O2 in vivo.
Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing
Authors: Clarissa Elizabeth Maria , Karmacharya Mamata , Choi Hyunmin , Kumar Sumit , Cho Yoon‐Kyoung 
Published: February 2025

AbstractThe advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐based genome editing technologies has opened up groundbreaking possibilities for treating a wide spectrum of genetic disorders and diseases. However, the success of these technologies relies heavily on the development of efficient and safe delivery systems. Among the most promising approaches are natural and synthetic nanocarrier‐mediated delivery systems, including viral vectors, extracellular vesicles (EVs), engineered cellular membrane particles, liposomes, and various nanoparticles. These carriers enhance the efficacy of the CRISPR system by providing a unique combination of efficiency, specificity, and reduced immunogenicity. Synthetic carriers such as liposomes and nanoparticles facilitate CRISPR delivery with high reproducibility and customizable functions. Viral vectors, renowned for their high transduction efficiency and broad tropism, serve as powerful vehicles for delivering CRISPR components to various cell types. EVs, as natural carriers of RNA and proteins, offer a stealth mechanism to evade immune detection, allowing for the targeted delivery of genome editors with minimal off‐target effects. Engineered cellular membrane particles further improve delivery by simulating the cellular environment, enhancing uptake, and minimizing immune response. This review explores the innovative integration of CRISPR genome editors with various nanocarrier systems, focusing on recent advancements, applications, and future directions in therapeutic genome editing.
A Simple and Efficient Non‐Noble Cathode Catalyst Based on Carbon Hollow Nanocapsules Containing Cobalt‐Based Materials for Anion Exchange Membrane Water Electrolyzer
Authors: Radhakrishnan Sivaprakasam , Ramakrishnan Shanmugam , Jayaraj Santhosh Kumar , Mamlouk Mohamed , Kim Byoung‐Suhk 
Published: February 2025

AbstractAn efficient approach for fabrication of non‐noble metal‐based electrocatalyst is desirable for designing the energy storage and conversion devices in real‐world usages due to low cost and excellent catalytic properties. The preparation of hollow carbon capsules (HCC) containing cobalt (Co)‐based electrocatalyst is reported by a simple synthesis process without using templates for the first time. Initially, cobalt phenylphosphonate (Co‐MOF) nanorods are fabricated through a simple hydrothermal approach. The as‐formed Co‐MOF is covered with a thin coating of polydopamine (DP‐Co‐MOF) through chemical polymerization of dopamine in Tris‐HCl (pH 8.5). The DP‐Co‐MOF is used as self‐degraded template for the formation of HCC under pyrolysis. The formation mechanism and hydrogen evolution reaction (HER) activity of HCC are investigated. The hollow structure derived under N2 exhibits a low overpotential (295 mV at 100 mA cm−2) with excellent stability (90.98%) for 150 h, which is further verified by density functional theory (DFT) calculations. Finally, the designed anion exchange membrane (AEM) water electrolyzer based on C─Co─N as cathode delivers a current density of 500 mA cm−2 (at 2.19 V) and 1000 mAcm−2 (at 2.33 V) in 1.0 m KOH at 60 °C. The fabricated new Co‐based electrocatalyst is highly beneficial for the fabrication of cost‐effective and high‐performance AEMWEs.
Folding Competition and Dynamic Transformation in DNA Origami: Parallel Versus Antiparallel Crossovers
Authors: Lee Jung Yeon , Yang Qi , Chang Xu , Perumal Devanathan , Zhang Fei 
Published: February 2025

AbstractDNA is a versatile abiomaterial for constructing nanostructures with biomedical and biotechnological applications. Among the methods available, DNA origami is a robust and widely recognized technique. Traditionally, most origami designs adopt antiparallel crossovers in both scaffold and staple strands, with less emphasis on parallel crossovers, which offer advantages like enhanced nuclease resistance and single‐strand routing potential. Here, a DNA origami nanostructure is designed, featuring two rotational panels that can be locked into configurations based on either antiparallel or parallel crossovers. By systematically varying the length and arrangement of these key staples, 36 pairs of antiparallel and parallel designs are studied in competitive folding tests, providing insights into the relative preference for each design. The 12 antiparallel and parallel designs are ranked, their folding pathways are examined, and nuclease resistance is assessed. The results reveal that the arrangement of staples near the central scaffold crossover is crucial for shifting between parallel and antiparallel conformations. Additionally, a two‐way isothermal transformation between antiparallel and parallel origami driven by toehold‐mediated displacement reactions is demonstrated, highlighting the potential of parallel designs as dynamic nanodevices for temperature‐sensitive environments. This study offers valuable insights into ‐ dynamics in antiparallel and parallel DNA origami, opening opportunities for designing nanodevices based on parallel crossovers.
A Long‐Life Zinc‐Bromine Single‐Flow Battery Utilizing Trimethylsulfoxonium Bromide as Complexing Agent
Authors: Zhao Ming , Cheng Tao , Li Tianyu , Xie Congxin , Yin Yanbin , Li Xianfeng 
Published: February 2025

AbstractAqueous zinc‐bromine single‐flow batteries (ZBSFBs) are highly promising for distributed energy storage systems due to their safety, low cost, and relatively high energy density. However, the limited operational lifespan of ZBSFBs poses a significant barrier to their large‐scale commercial viability. Here, trimethylsulfoxonium bromide (TMSO), a nonquaternary ammonium salt, is introduced as a bromine complexing agent to extend the cycle life of ZBSFBs by reducing the imbalance of active substances. Benefiting from the strong interaction between TMSO and H2O, the hydrogen evolution reaction is notably suppressed compared with the traditional N‐ethyl‐N‐methyl‐pyrrolidinium bromide (MEP) complexing agent, resulting in reduced bromine accumulation at the cathode. The resultant solid polybromide‐TMSO complex, featuring rapid electrochemical redox reaction of Br2/Br−, further contributes to reduce the residual bromine. Consequently, the ZBSFB with TMSO demonstrates a longer lifespan of 1500 cycles with a higher average energy efficiency (EE) of ≈81.6% than that with MEP (less than 300 cycles with an average EE of ≈80.2%). This research explores a sulfonium complexing agent and provides a feasible strategy to effectively extend the cycle life of ZBSFBs.
Exploring heterogeneous responses of residential water consumption to lawn size: Insights from a novel parcel‐level dataset
Authors: Stitzel Brandli , Pjesky Rex 
Published: February 2025

AbstractThis paper investigates the relationship between residential water consumption and lawn watering, considering precipitation, temperature, evapotranspiration, and lawn size. This paper uses a unique dataset comprising water‐billing information, parcel features, and weather data to explore how lawn dimensions drive water usage. The findings highlight the necessity of considering these factors in models of residential water demand and informing water conservation strategies. Two general findings emerge: Summer water consumption increases by 37% with a 10% expansion in lawn size, and monthly usage drops 52 gallons for each extra centimeter of precipitation in the preceding 30 days. Furthermore, the study examines the heterogeneity of responses among homeowners with varying household characteristics. The paper shows that newer homes have water consumption more strongly influenced by lawn size during summer, and higher‐value homes with larger lawns exhibit greater sensitivity to lawn size in the summer.
Microfluidics for Electrochemical Energy Conversion and Storage: Prospects Toward Sustainable Ammonia Production
Authors: Rems Ervin , Herceg Ana , Yordanova Apostolova Desislava , Dominko Robert , Jovanovič Primož , Genorio Bostjan 
Published: February 2025

AbstractAmmonia is a key chemical in the production of fertilizers, refrigeration and an emerging hydrogen‐carrying fuel. However, the Haber‐Bosch process, the industrial standard for centralized ammonia production, is energy‐intensive and indirectly generates significant carbon dioxide emissions. Electrochemical nitrogen reduction offers a promising alternative for green ammonia production. Yet, current reaction rates remain well below economically feasible targets. This work examines the application of electrochemical microfluidics for the enhancement of the rates of electrochemical ammonia synthesis. The review is built on the introduction to electrochemical microfluidics, corresponding cell designs, and the main applications of microfluidics in electrochemical energy conversion/storage. Based on recent advances in electrochemical ammonia synthesis, with an emphasis on the critical role of robust experimental controls, electrochemical microfluidics represents a promising route to environmentally friendly, on‐site and on‐demand ammonia production. This review aims to bridge the knowledge gap between the disciplines of electrochemistry and microfluidics and promote interdisciplinary understanding and innovation in this transformative field.
Microalgal‐bacterial aggregates for wastewater treatment: Origins, challenges, and future directions
Authors: Hammond Charles R. , Hernández Misael Sebastián Gradilla , Loge Frank J. 
Published: February 2025

AbstractMicroalgal‐bacterial aggregates are promising for wastewater treatment because they remove organic matter, nitrogen, and phosphorus while producing biomass that settles quickly. This review details the development of microalgal‐bacterial aggregates, identifies key challenges, and proposes future research directions. While many studies have been performed in the laboratory with synthetic wastewater and artificial lighting, more research is needed to better understand how to form and sustain aggregates at larger scales with real wastewater and natural lighting. While it appears that microalgal‐bacterial aggregates are unlikely to replace or augment conventional activated sludge, they have the potential to improve resource recovery in existing microalgae‐based wastewater treatment processes (e.g., high‐rate algal ponds). Alternatively, attached‐growth bioreactors utilizing microalgal‐bacterial consortia may be able to compete directly with conventional activated sludge while providing the benefits that microalgae offer, although additional research is needed.Practitioner Points
More pilot and full‐scale research on microalgal‐bacterial processes is needed.
Microalgae cultivation with short retention times is challenging.
Attached‐growth processes may allow for competitive footprint requirements.
Adsorption behavior of levofloxacin hydrochloride on non‐degradable microplastics aging with H2O2
Authors: Li Yinghua , Wang Yiyan , Yang Shutong , Bao Terun , Su Fei , Qian Jie 
Published: February 2025

AbstractPlastics pollutants, especially microplastics (MPs, <5 mm in diameter) and levofloxacin hydrochloride (Lev‐HCl) often co‐exist in the aquatic environment. To explore the adsorption processes and mechanisms of Lev‐HCl by non‐degradable MPs, in this study, H2O2 oxidation was used to age polyvinyl chloride (PVC), polystyrene (PS), and polyethylene terephthalate (PET) MPs. The results demonstrated that the equilibrium adsorption capacity increased significantly after aging, as H2O2‐PET (1.167 mg/g) > PET (0.995 mg/g), H2O2‐PS (1.057 mg/g) > PS (0.957 mg/g), H2O2‐PVC (1.107 mg/g) > PVC (0.975 mg/g). After H2O2 aging, the hydrogen bond (‐OH) was more obvious, and π‐π interactions were significantly enhanced. These non‐degradable MPs mainly adsorbed Lev‐HCl by micropore filling (contributions: PVC 65.9%, PS 56%, PET 63.5%). The current study highlights the potential of non‐degradable MPs to act as a vector for Lev‐HCl in the aquatic environment, especially after H2O2 aging.Practitioner Points
Adsorption behavior of Lev‐HCl onto three non‐degradable MPs was elucidated.
The adsorption capacity increased significantly after aging for PVC, PS, and PET MPs.
The hydrogen bonding and π‐π interactions of H2O2‐aged MPs were more significant.
Multi‐layer adsorption on non‐homogeneous surfaces via micropore filling was revealed.
Assessment of pollution in Alibeykoy Dam Lake (Istanbul, Türkiye) and its influent streams: Phytoplankton composition and heavy metal accumulation
Authors: Yilmaz Nese , Dogan Ilhan , Demir Goksel , Yalcin Ibrahim Ertugrul , Khan Saifullah , Ozyigit Ibrahim Ilker 
Published: February 2025

AbstractIn this study, the pollution levels of Alibeykoy Dam Lake were assessed by examining phytoplankton distribution, physicochemical parameters, nutrient content, and heavy metal concentrations. Alibeykoy Dam is a critical drinking water source for the Istanbul metropolitan area. Water samples were collected from the lake and six influent streams (Cebeci, Pirincci, Sidan, Bolluca, Gulgen, and Kocaman) in January, February, May, and September of 2021. A total of 36 taxa from seven divisions were identified, including Bacillariophyta (14), Charophyta (2), Chlorophyta (9), Cryptophyta (1), Cyanobacteria (3), Euglenozoa (4), and Miozoa (3). Diatoms were found to be the dominant group in terms of species richness and abundance. The dominant species recorded in each site were as follows: Microcystis aeruginosa in Alibeykoy Dam and Sidan Creek, Cyclotella ocellata in Cebeci and Gulgen Creeks, Scenedesmus sp. in Pirincci Creek, Navicula cryptocephala in Bolluca Creek, and Sphaerocystis planctonica in Kocaman Creek. Mesotrophic and eutrophic phytoplankton species suggested that the lake is nearing eutrophic conditions. This conclusion was further supported by high concentrations of heavy metals and nutrients detected in the water samples. This research is significant because it provides a comprehensive understanding of the ecological status of Alibeykoy Dam Lake, a critical resource for drinking water in Istanbul. The study highlights potential risks associated with eutrophication and heavy metal accumulation by identifying pollution levels and dominant species. These findings are vital for implementing effective water resource management strategies, ensuring the lake's sustainability, and protecting public health.Practitioner Points
The pollution rate of Alibeykoy Dam Lake was determined.
The presence of mesotrophic and eutrophic species of phytoplankton indicated the lake's trophic structure.
The measurements were done to estimate the lake's heavy metal and nutrient contents.
A Review of Tree‐Based Methods for Analyzing Survey Data
Authors: Bhaduri Diya , Toth Daniell , Holan Scott H. 
Published: February 2025

ABSTRACTRecent advances in data complexity and availability present both challenges and opportunities for automated data exploration. Tree‐based methods, known for their interpretability, are widely used for building regression and classification models. However, they often lag behind the best supervised learning approaches in terms of prediction accuracy. To address this limitation, ensemble methods, such as random forests, combine multiple trees to improve prediction accuracy, though at the cost of interpretability. While tree‐based methods have seen extensive use in various fields, their application in the context of complex survey data has been relatively limited. This article provides an overview of the state‐of‐the‐art tree‐based approaches for analyzing complex survey data. It distinguishes methods explicitly designed for survey contexts from those adapted from other domains. The discussion covers applications in model‐assisted approaches, disclosure limitation, and small area estimation, as well as other recent methodological developments tailored to survey data. Additionally, the article explores aggregated tree models that sacrifice interpretability for improved prediction accuracy. These models, such as Bagging, Random Forests, and Boosting, are explained, along with the concept of out‐of‐bag error for model evaluation. Finally, this article provides the history and development of tree models, from their origins in regression trees to more recent Bayesian approaches, and aggregated tree models. This overview sheds light on the potential utility of tree‐based methods in survey methodology and provides insights into future research directions in this evolving field.
Dusky Gopher Frog (Lithobates sevosus) Repatriation at a Reintroduction Site Through Zoo‐Led Captive‐Release Efforts
Authors: Bogisich Allison , Candia Ana Karen , Cantrell Jessica , Collins Cassandra , Reichling Steven B. , Poo Sinlan 
Published: February 2025

ABSTRACTCaptive‐release programs are an increasingly popular conservation strategy to combat wild extinctions. However, it is critical to determine if translocating animals from captive colonies (“source populations”) leads to the establishment of new wild populations that are both stable and self‐sustaining. To fill this knowledge gap, we provide a case study from the dusky gopher frog (Lithobates sevosus) reintroduction program to serve as an example for other critically endangered amphibians. In this study, we provide quantitative information on the reintroduction and survivorship of zoo‐bred individuals that are released into the wild. This unique opportunity is the culmination of close to 20 years of collective efforts across multiple agencies. By taking advantage of the key monitoring window shortly after initial releases, we can formally declare the first successfully reintroduced, breeding population of dusky gopher frogs founded solely from a captive‐bred colony.
Moscow Martinists as the Providers of the Rosicrucian Enlightenment in Russia: Enquiries into the Emergence of the Hermetic Library
Authors: Morosow Witalij 
Published: February 2025

Abstract
This chapter explores the history of the emergence of the Hermetic Library, demonstrating how interest in alchemy in Imperial Russia intensified in the last quarter of the 18th century among the Moscow Martinists from the circle of Nikolay Novikov. The research reveals that, initially, this interest was associated with the development of ideas on natural philosophy and theosophy characteristic of Prussian Rosicrucians. It was these connections with Prussian political figures (Woellner, Bischoffswerder) that led to Novikov’s arrest in 1792 and the subsequent crackdown on Rosicrucians in Russia. However, after Novikov’s release, a large-scale project was launched to create the first manuscript library of alchemical literature in Russian, known as the Hermetic Library. The chapter examines how the Martinists’ perception of alchemical thought evolved from 1782 to 1820s and how the Hermetic Library project became instrumental in revealing the concept of philosophia perennis.
Service Design in the Context of Pragmatic Governance: Cases of the Singapore Government
Authors: Lee Jung-Joo , Ng Debbie 
Published: February 2025

Abstract
Since its initial collaboration with the globally renowned design consultancy IDEO in 2009, the Singapore government has established itself as a rapid adopter of design among Asian governments. By examining service design as a socio-cultural practice, this chapter reviews the trajectory of the Singapore government’s design adoption within the framework of pragmatic governance. Through interviews with public officers and case studies, it becomes evident that their design adoption journey has been characterized by pragmatic acculturation, a legacy of the Singapore government. This chapter aims to illustrate how the adoption and impact of service design are influenced by socio-cultural and political contexts. While pragmatic governance provides the rationale for the Singapore government’s design adoption journey, it also exposes critical limitations, notably a partial understanding of design. Our discussion will explore strategies to mitigate this partial understanding within the context of pragmatic governance.
Description of the behavioural contexts of underwater sound production in juvenile green turtles Chelonia mydas
Authors: Maucourt Léo , Charrier Isabelle , Huetz Chloé , Aubert Nathalie , Bourgeois Ouvéa , Jeantet Lorène , Lecerf Nicolas , Lefebvre Fabien , Lelong Pierre , Lepori Muriel , Martin Jordan , Régis Sidney , Chevallier Damien 
Published: February 2025

Abstract
Green sea turtles Chelonia mydas have the ability to hear and produce sounds under water, with some of them potentially involved in social communication. To investigate the potential biological function of these sounds, we used a combination of acoustic, video and multi-sensor recordings of 23 free-ranging juvenile green turtles and we examined the co-occurrences of sounds with behaviours or external events. Our study revealed that most of the sounds were produced when the sea turtles were resting or swimming. However, four sound types were produced in more specific contexts. Long sequences of rumbles were recorded after sunset and mainly during resting. All these rumbles appear to have been produced by several individuals recorded simultaneously, suggesting that rumbles may be used for social interactions. The frequency modulated sound was highly associated with scratching behaviour. The grunt that was produced occasionally when green turtles were vigilant or approaching a conspecific. The long squeak was produced significantly by a small number of individuals in the presence of humans. The grunt and the long squeak may be the first evidence of an alarm or warning signal for intra-specific communication in green turtles. Our results mark a significant milestone in advancing the understanding of sound production in the behavioural ecology of sea turtles. Further experimental investigations (i.e., playback experiments) are now required to test the hypotheses suggested by our findings. Warning signals could be used to prevent sea turtles of a danger and may contribute to their conservation.