bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of donor-derived cell-free DNA in the setting of multiple kidney transplantations
Authors: Pettersson Linnea , Frischknecht Lukas , Westerling Sofia , Ramezanali Hamid , Weidmann Lukas , Lopez Kai Castrezana , Schachtner Thomas , Nilsson Jakob 
Published: February 2024

BackgroundThe routine use of donor-derived cell-free DNA (dd-cfDNA) assays to monitor graft damage in patients after kidney transplantation is being implemented in many transplant centers worldwide. The interpretation of the results can be complicated in the setting of multiple sequential kidney transplantations where accurate donor assignment of the detected dd-cfDNA can be methodologically challenging.MethodsWe investigated the ability of a new next-generation sequencing (NGS)-based dd-cfDNA assay to accurately identify the source of the detected dd-cfDNA in artificially generated samples as well as clinical samples from 31 patients who had undergone two sequential kidney transplantations.ResultsThe assay showed a high accuracy in quantifying and correctly assigning dd-cfDNA in our artificially generated chimeric sample experiments over a clinically meaningful quantitative range. In our clinical samples, we were able to detect dd-cfDNA from the first transplanted (nonfunctioning) graft in 20% of the analyzed patients. The amount of dd-cfDNA detected from the first graft was consistently in the range of 0.1%–0.6% and showed a fluctuation over time in patients where we analyzed sequential samples.ConclusionThis is the first report on the use of a dd-cfDNA assay to detect dd-cfDNA from multiple kidney transplants. Our data show that a clinically relevant fraction of the transplanted patients have detectable dd-cfDNA from the first donor graft and that the amount of detected dd-cfDNA is in a range where it could influence clinical decision-making.
Exosomal small RNA profiling in first-trimester maternal blood explores early molecular pathways of preterm preeclampsia
Authors: Gál Luca , Fóthi Ábel , Orosz Gergő , Nagy Sándor , Than Nándor Gábor , Orbán Tamás I. 
Published: February 2024

IntroductionPreeclampsia (PE) is a severe obstetrical syndrome characterized by new-onset hypertension and proteinuria and it is often associated with fetal intrauterine growth restriction (IUGR). PE leads to long-term health complications, so early diagnosis would be crucial for timely prevention. There are multiple etiologies and subtypes of PE, and this heterogeneity has hindered accurate identification in the presymptomatic phase. Recent investigations have pointed to the potential role of small regulatory RNAs in PE, and these species, which travel in extracellular vesicles (EVs) in the circulation, have raised the possibility of non-invasive diagnostics. The aim of this study was to investigate the behavior of exosomal regulatory small RNAs in the most severe subtype of PE with IUGR.MethodsWe isolated exosomal EVs from first-trimester peripheral blood plasma samples of women who later developed preterm PE with IUGR (n=6) and gestational age-matched healthy controls (n=14). The small RNA content of EVs and their differential expression were determined by next-generation sequencing and further validated by quantitative real-time PCR. We also applied the rigorous exceRpt bioinformatics pipeline for small RNA identification, followed by target verification and Gene Ontology analysis.ResultsOverall, &gt;2700 small RNAs were identified in all samples and, of interest, the majority belonged to the RNA interference (RNAi) pathways. Among the RNAi species, 16 differentially expressed microRNAs were up-regulated in PE, whereas up-regulated and down-regulated members were equally found among the six identified Piwi-associated RNAs. Gene ontology analysis of the predicted small RNA targets showed enrichment of genes in pathways related to immune processes involved in decidualization, placentation and embryonic development, indicating that dysregulation of the induced small RNAs is connected to the impairment of immune pathways in preeclampsia development. Finally, the subsequent validation experiments revealed that the hsa_piR_016658 piRNA is a promising biomarker candidate for preterm PE associated with IUGR.DiscussionOur rigorously designed study in a homogeneous group of patients unraveled small RNAs in circulating maternal exosomes that act on physiological pathways dysregulated in preterm PE with IUGR. Therefore, our small RNA hits are not only suitable biomarker candidates, but the revealed biological pathways may further inform us about the complex pathology of this severe PE subtype.
Integrating clinical and cross-cohort metagenomic features: a stable and non-invasive colorectal cancer and adenoma diagnostic model
Authors: Zhou Dan , Chen Youli , Wang Zehao , Zhu Siran , Zhang Lei , Song Jun , Bai Tao , Hou Xiaohua 
Published: February 2024

Background: Dysbiosis is associated with colorectal cancer (CRC) and adenomas (CRA). However, the robustness of diagnostic models based on microbial signatures in multiple cohorts remains unsatisfactory.Materials and Methods: In this study, we used machine learning models to screen metagenomic signatures from the respective cross-cohort datasets of CRC and CRA (selected from CuratedMetagenomicData, each disease included 4 datasets). Then select a CRC and CRA data set from the CuratedMetagenomicData database and meet the requirements of having both metagenomic data and clinical data. This data set will be used to verify the inference that integrating clinical features can improve the performance of microbial disease prediction models.Results: After repeated verification, we selected 20 metagenomic features that performed well and were stably expressed within cross-cohorts to represent the diagnostic role of bacterial communities in CRC/CRA. The performance of the selected cross-cohort metagenomic features was stable for multi-regional and multi-ethnic populations (CRC, AUC: 0.817–0.867; CRA, AUC: 0.766–0.833). After clinical feature combination, AUC of our integrated CRC diagnostic model reached 0.939 (95% CI: 0.932–0.947, NRI=30%), and that of the CRA integrated model reached 0.925 (95%CI: 0.917–0.935, NRI=18%).Conclusion: In conclusion, the integrated model performed significantly better than single microbiome or clinical feature models in all cohorts. Integrating cross-cohort common discriminative microbial features with clinical features could help construct stable diagnostic models for early non-invasive screening for CRC and CRA.
Expression of HER2 in high-grade urothelial carcinoma based on Chinese expert consensus and the clinical effects of disitamab vedotin-tislelizumab combination therapy in the treatment of advanced patients
Authors: Zhu Kejia , Chang Yao , Zhao Delong , Guo Andong , Cao Jishuang , Wu Chenrui , Guan Yong , Ding Sentai 
Published: February 2024

Background: A vast number of researchers have discovered high levels of human epidermal growth factor receptor-2 (HER2) expression in urothelial carcinoma (UC), but they do not use a uniform scoring system. Based on the 2021 edition of clinical pathological expert consensus on HER-2 testing in UC in China, we investigated the expression level and clinical significance of HER2 in high-grade UC. Furthermore, we looked at the prognosis of patients with locally advanced/metastatic UC after combining HER2 targeting antibody-drug conjugates (ADC) medication disitamab vedotin (DV) with programmed cell death protein 1 (PD-1) inhibitor tislelizumab.Patients and methods: From 2019 to 2022, we collected paraffin specimens of UC from the Department of Urology at the Provincial Hospital Affiliated to Shandong First Medical University. HER2 expression-related factors were investigated. Patients with advanced UC who have failed systemic chemotherapy at least once and had received immune checkpoint inhibitor (ICI) medication during second-line treatment were selected and treated with DV in combination with tislelizumab. We assessed the therapy’s efficacy and safety.Results: 185 patients with high-grade UC were included in this investigation. 127 patients (68.7%) were HER2 positive (IHC 2+/3+) according to the 2021 Clinical pathological expert consensus on HER2 testing in UC in China. The clinical stage of UC differed statistically significantly between the HER2-and HER2+ groups (p = 0.019). Sixteen advanced UC patients were treated with DV and tislelizumab for a median of 14 months. The disease control rate was 87.5%, while the objective response rate (ORR) was 62.5%. The ORR of HER2+ individuals was higher than that of HER2-individuals (70.0% vs. 50.0%). The median progression-free survival or overall survival was not reached. In this study, the incidence of treatment-related adverse events was 68.8% (11/16), with all of them being grade 1 or 2 adverse reactions.Conclusion: HER2 protein expressed at a high percentage in UC, and 68.7% patients expressed HER2 positive (IHC 2+/3+). HER2+ expression is positively correlated with higher clinical stage of UC. HER2 targeted ADC drug disitamab vedotin combining with PD-1 inhibitor tislelizumab has shown efficacy, safety and controllable adverse reactions in the treatment of advanced UC.
Tackling suppressive cancer microenvironment by NARF-derived immune modulatory vaccine and its validation using simulation strategies
Authors: Paranthaman Priyanga , Veerappapillai Shanthi 
Published: February 2024

Introduction: Targeting tumor microenvironment is beneficial and present an ideal setting for the development of futuristic immunotherapy. Here, we make use of Nuclear prelamin A recognition factor (NARF), a protein linked to the coactivation of transcriptional regulators in human breast cancer stem cells (CSC) in our investigation.Methods: In this study, we initially computed the epitope regions possessing the ability to stimulate both T and B cells within the NARF protein. These identified epitope areas were fused with an adjuvant such as RpfB and RpfE as well as linkers like AAY, GPGPG, KK, and EAAAK. The constructed vaccine was further characterized by assessing its physicochemical properties and population coverage. The potential interactions of the designed vaccine with different toll-like receptors were examined by a sequence of computational studies. Of note, docking study were employed to understand its mechanism of action. Molecular dynamics and immune simulation studies were conducted to comprehend more into their structural stability and immune responses. The resultant vaccine was back-translated, codon-optimised and introduced into pET-28 (+) vector.Results and discussion: We hypothesize from the results that the designed NARF protein-based vaccine in our analysis could effectively provoke the immune responses in the target organism through TLR-7 binding and promotes MHC class-II mediated antigen presentation. Indeed, comprehensive evaluations conducted in both in vitro and in vivo settings are imperative to substantiate the safety and efficacy of the developed vaccine.
La evaluación formativa en el nivel primaria en América Latina: Una revisión sistemática
Authors: Quico-Quispe Rosalinda Isabel , Lescano-López Galia Susana , Boy-Barreto Ana Maritza , Olivos-Jimenez Luis Marcelo 
Published: February 2024

El objetivo de la presente revisión sistemática fue analizar las características de la evaluación formativa en el nivel primario en América Latina, a través de artículos científicos que fueron ubicados en revistas indexadas en base de datos Scopus, Web of Science y Scielo, divulgados durante los años 2018 y 2023. Se realizó una revisión de los artículos científicos considerando los protocolos sugeridos por el modelo PRISMA “Preferred Reporting Items for Systematic reviews and Meta-Analyses”. Se concluye que existe una limitada producción científica relacionada con la evaluación formativa, en educación primaria en Latinoamérica, y que la evaluación formativa brinda la oportunidades para aprender de mejor manera generando climas positivos y buenas relaciones. La retroalimentación está inmersa en la evaluación formativa y está centrada en el estudiante buscando su aprendizaje y por ende su bienestar.
ANALISIS PERBANDINGAN PEMBELIAN ONLINE DAN OFFLINE TERHADAP KEPUTUSAN PEMBELIAN PADA MINIMARKET INDOMARET DI KECAMATAN MANGGALA
Authors: Putri Rezky Amalia , Chahyono Chahyono , Ruslan Muhlis 
Published: February 2024

Tujuan penelitian untuk mengetahui dan mendeskripsikan mengenai bagaiamana perbandingan antara pembelian online dan offline terhadap keputusan pembelian pada minimarket indomaret di Kecamatan Manggala. Pada dasarnya tipe penelitian yang digunakan adalah deskriptif yaitu sebuah penelitian yang berusaha memberikan gambaran mengenai objek yang diteliti yang bertujuan membuat deskriptif atau gambaran secara sistematis dan aktual mengenai fakta-fakta yang ada. Pemilihan informan dilakukan dengan cara purposive sampling yaitu; penarikan informan yang dilakukan secara sengaja dengan kriteria tertentu. Adapun subyek penelitian ini berjumlah 10 orang Yaitu orang atau pelaku yang benar-benar tahu dan menguasai masalah, serta terlibat langsung dengan masalah penelitian. Teknik pengumpulan data dalam penelitian ini menggunakan observasi, wawancara, dokumentasi. Hasil penelitian menunjukkan bahwa faktor-faktor yang mempengaruhi Keputusan Pembelian Konsumen pada minimarket Indomaret di Kacamatan Manggala yaitu produk, Harga, promosi, pelayanan, Fasilitas dan Lokasi.
This study aimed to determine and describe the comparison between online and offline purchase on purchasing decision in Indomaret minimarket in Manggala Sub-district. Basically the type of research used is descriptive, namely a study that attempts to provide an overview of the object being studied which aims to make a descriptive or systematic and actual description of the facts that exist. The selection of informants was carried out by purposive sampling namely, intentional withdrawal of informant with certain criteria. The subjects of this study amounted to 10 people, namely people or actor who really know and master the problem, and they were directly involved with the research problem. Data collection technique in this study used observation, interviews, documentation. The results of the study showed that the factors that influence consumer purchasing decision at Indomaret minimarket in the Manggala Sub-district are product, price, promotion, service, facility and location.
EKSISTENSI YAYASAN PESANTREN K.H MUHAMMAD SYAWIR DAHLAN DALAM MENGEMBANGKAN USAHA EKONOMI MIKRO DIKOTA PANGKAJE’NE DAN KEPULAUAN
Authors: Dahlan Muhammad Syawir , Saleh Haeruddin , Mardjuni Sukmawati 
Published: February 2024

Penelitian bertujuan Untuk mengetahui seberapa tinggi peran dari Yayasan dalam membantu meningkatkan perekonomian masyarakat selain itu juga untuk mengetahui perubahan ekonomi masyarakat dengan adanya bantuan Yayasan pesantren K.H Muhammad Syawir Dahlan. yayasan adalah suatu badan hukum, yang dilahirkan oleh suatu pernyataan sepihak; pernyataan itu harus berisikan pemisahan suatu kekayaan untuk tujuan tertentu, dengan penunjukan bagaimana kekayaan itu harus diurus dan dipergunakan. Penelitian ini menggunakan metode kualitatif, yang membahas perilaku, fenomena, peristiwa, masalah, atau keadaan tertentu yang menjadi fokus penyelidikan dan menghasilkan deskripsi kalimat yang bermakna yang memberikan pemahaman tertentu. Pendekatan pemeriksaan, yang ditujukan kepada yayasan dan orang-orang secara karakteristik dan menyeluruh agar tidak mengurung orang atau perkumpulan menjadi variabel/spekulasi. Hasil dari penelitian ini menunjukkan bahwa Yayasan KH. Muhammad Syawir Dahlan sangat memberikan dampak yang positif dalam memajukan UMKM di Pangkajene.
This study aimed to determine how high the role of the Foundation is in helping to improve the community's economy as well as to find out changes in the community's economy with the assistance of the K.H Muhammad Syawir Dahlan Islamic Boarding School Foundation. Foundation is a legal entity, which was born by a unilateral statement; the statement must contain a separation of a property for a specific purpose, with an indication of how the wealth must be managed and used. This research used a qualitative method, which discusses certain behaviors, phenomena, event, problem, or certain circumstance that are the focus of the investigation and produce meaningful sentence description that provides a certain understanding. Examination approach, which is aimed at foundation and people in a characteristic and comprehensive manner so it is not to confine people or association to variables/speculation. The results of this study indicated that the KH. Muhammad Syawir Dahlan has a very positive impact on advancing MSMEs in Pangkaje’ne.
ІНТЕГРАЛЬНІ РІВНЯННЯ ЗАДАЧІ ДИФРАКЦІЇ ХВИЛЬ У ПРУЖНИХ СЕРЕДОВИЩАХ З ВКЛЮЧЕННЯМИ ЗА ДІЇ НЕСТАЦІОНАРНИХ НАВАНТАЖЕНЬ
Authors: Швабюк В.І. , Фурс Т.В. , Коменда Н.В. , Мікуліч С.Б. 
Published: February 2024

У роботі розроблено алгоритм реалізації непрямого підходу методу граничних елементів до дослідження напружено-деформованого стану для тіл з включеннями за дії нестаціонарних навантажень. Використання методу інтегральних перетворень у випадку дії змінних у часі навантажень дозволило звести розв’язання нестаціонарної задачі до скінченної системи задач у частотній області. У роботі отримано потенціальні представлення фундаментальних функцій для плоскої динамічної задачі теорії пружності. Використовуючи основні підходи методу граничних елементів, у роботі отримано інтегральні рівняння другої основної задачі для тіл з включеннями. Встановлено, що ядра отриманих інтегральних рівнянь містять сингулярну та гіперсингулярну особливості.
ЗАХОДИ ЩОДО РОЗШИРЕННЯ ВИКОРИСТАННЯ ГАЗОВИХ ПАЛИВ В ТЕХНОЛОГІЧНИХ ТРАНСПОРТНИХ ЗАСОБАХ
Authors: Захарчук В.І. , Захарчук О.В. , Швабюк В.В. , Ярощук В.В. 
Published: February 2024

Метою роботи є розробка заходів для забезпечення можливості ефективної експлуатації колісних тракторів та інших технологічних транспортних засобів на газових паливах. Переобладнано дизель Д-243 в газовий двигун з іскровим запалюванням та виконано його стендові випробування, які підтвердили його роботоздатність та показали хороші експлуатаційні показники. Потужність газового двигуна на рівні дизеля, сумарна токсичність відпрацьованих газів в 1,8 рази менша у порівнянні з дизелем, але витрата палива є більшою в газового двигуна. За тією ж технологією і з використанням тих же елементів переобладнано трактор МТЗ-80 для роботи на газових паливах. Виконані його дорожні випробування, які показали, що є певні особливості керування газовим двигуном та трансмісією такиого трактора. Для забезпечення ефективної експлуатації технологічних транспортних засобів на газових паливах пропонується: встановлення високоенергетичної системи запалювання; розробка системи передпускового підігріву газового редуктора; розробка способів забезпечення технологічних транспортних засобів газовим паливом.
Academic Education in the Era of Generative Artificial Intelligence
Authors: Vafadar Maryam , Moradi Amani Ali 
Published: February 2024

This paper provides a technical review of the Generative AI technology and its challenges and opportunities in the education sector. Generative Artificial Intelligence (GAI), presented in some tools such as ChatGPT, has been continuously penetrating our normal lives. It has also attracted several research efforts, from both academia and industry researchers, to solve real-world problems in different applications such as finance and health. Generative AI is indeed a type of Artificial Intelligence that can efficiently "create" a wide variety of information in the form of images, videos, audio, text, and 3D models. Therefore, they can facilitate data analysis and visualization, and enhance personalized and adaptive learning in the education sector. Although instructors and teachers will not be substituted by Generative AI robots completely, education and academic delivery of courses are expected to experience a revolution in the presence of GAI. Similar to other new technologies, serious potential challenges and opportunities are expected in employing GAI in the education sector.
L’évolution des consommations alimentaires dans le monde
Authors: Van Hecke Etienne 
Published: February 2024

Les bilans alimentaires de la FAO permettent de comparer les consommations alimentaires par pays ou par sous-continent. Comme les premiers bilans alimentaires datent des années 1960, ils permettent également de retracer l’évolution de la consommation sur une longue période. Le texte et les tableaux sont soutenus par des graphiques qui permettent d’un coup d’œil d’observer la composition et son évolution. Les graphiques montrent la diversité des régimes alimentaires ainsi que de fortes différences des quantités absorbées aussi bien dans l’espace que dans le temps. Tandis que la consommation totale atteint 3 800 kcal/jour en Amérique du Nord, elle n’atteint pas les 2 300 kcal en Afrique centrale et de l’Est. La surconsommation aussi bien de calories que de protéines d’un côté contraste avec une sous-alimentation dans une grande partie de l’Afrique. Les écarts ont augmenté dans le courant du dernier demi-siècle. Dans les sous-continents avec une consommation de plus de 3 000 kcal/jour, celle-ci est non seulement trop élevée, mais de plus ne correspond pas à une alimentation saine comme avancée par l’OMS : trop de protéines animales, trop de graisses, trop de sucre.
Onsite Adsorbent Testing Approach for Challenging Condensate Mercury Removal Unit Design
Authors: Lepine Y. , Chaiwiboonpol P. , Danuthai T. , Paerungruang A. , Taenumtrakul T. , Porcheron F. 
Published: February 2024

Abstract
A test skid was designed and installed onsite to simultaneously pilot test multiple commercial adsorbents for mercury removal from a condensate feed. A slipstream of the condensate directly sampled from the existing plant (side stream test concept) was used for the test campaign. The respective performance of each adsorbent was determined during the test.
To gauge the presence of particulate mercury in the condensate feed, and to prevent sand, sludge and water carry-over to the different adsorbents tested, four stages of filtration and water coalescence were implemented into the test skid. Daily mercury analyses were conducted.
Following a one-month pilot test campaign, the spent adsorbents were then retrieved, analyzed, and tested to assess the performance and stability of each product. Several adsorbents were severely impacted by the test conditions, losing their mechanical strength or their active phase, or being highly contaminated with heavy hydrocarbons.
Overall, the test results showed a limited efficiency of all the adsorbents tested. The mercury adsorption capacity was found relatively low due to a high level of contamination for all the adsorbents tested. The adsorbents lifetime was estimated based on the unit pre-sizing, the test results and average feed conditions, anticipating high operating costs for this unit.
Adding Depth to Decarbonization: Advancements in Subsea Carbon Capture Injection and Storage for a Net Zero Future
Authors: Yeaw S. , Boersheim E. C. 
Published: February 2024

Abstract
In response to the accelerated climate change witnessed in recent years, caused by excessive Carbon Dioxide (CO2) emissions, the energy sector has adopted low carbon strategies to tackle these issues. One such approach involves implementing offshore Carbon Capture and Storage (CCS) using established subsea processing technologies as the foundational elements.
This paper briefly describes how subsea technology could be utilized within the Carbon Capture and Storage (CCS) domain, to tackle climate change and ensuring energy security. For instance, high carbon emitting assets can now be economically and environmentally developed, utilizing subsea carbon capture and reinjection technologies.
Considering the emerging status of the offshore Carbon Capture and Storage (CCS) sector, the objective of this paper is to present the latest advancements in offshore CCS. It will encompass an overview of development options, the technology qualification activities on key components, and insights into global offshore CCS initiatives.