bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell type specific long non-coding RNA targets identified by integrative analysis of single-cell and bulk colorectal cancer transcriptomes
Authors: Mihaljevic Ante , Rubin Philip , Chouvardas Panagiotis , Esposito Roberta 
Published: January 2024

AbstractLong non-coding RNAs (lncRNAs) represent an emerging class of genes which play significant and diverse roles in human cancers. Nevertheless, the functional repertoires of lncRNAs in cancer cell subtypes remains unknown since most studies are focused on protein coding genes. Here, we explored the contribution of lncRNAs in Colorectal Cancer (CRC) heterogeneity. We analyzed 49,436 single-cells from 29 CRC patients and showed that lncRNAs are significantly more cell type specific compared to protein-coding genes. We identified 996 lncRNAs strongly enriched in epithelial cells. Among these, 98 were found to be differentially expressed in tumor samples compared to normal controls, when integrating 270 bulk CRC profiles. We validated the upregulation of two of them (CASC19 and LINC00460) in CRC cell lines and showed their involvement in CRC proliferation by CRISPR-Cas9 knock down experiments. This study highlights a list of novel RNA targets for potential CRC therapeutics, substantiated through experimental validation.
Large-scale computational analyses of gut microbial CAZyme repertoires enabled by Cayman
Authors: Ducarmon Q. R. , Karcher N. , Tytgat H.L.P. , Delannoy-Bruno O. , Pekel S. , Springer F. , Schudoma C. , Zeller G. 
Published: January 2024

AbstractCarbohydrate-active enzymes (CAZymes) are crucial for digesting glycans, but bioinformatics tools for CAZyme profiling and interpretation of substrate preferences in microbial community data are lacking. To address this, we developed a CAZyme profiler (Cayman) and a hierarchical substrate annotation scheme. Leveraging these, we genomically survey CAZymes in human gut microbes (n=107,683 genomes), which suggests novel mucin-foraging species. In a subsequent meta-analysis of CAZyme repertoires in Western versus non-Western gut metagenomes (n=4,281) we find that non-Western metagenomes are richer in fibre-degrading CAZymes despite lower overall CAZyme richness. We additionally pinpoint the taxonomic drivers underlying these CAZyme community shifts. A second meta-analysis comparing colorectal cancer patients (CRC) to controls (n=1,998) shows that CRC metagenomes are deprived of dietary fibre-targeting, but enriched in glycosaminoglycan-targeting CAZymes. A genomic analysis of co-localizing CAZyme domains predicts novel substrates for CRC-enriched CAZymes. Cayman is broadly applicable across microbial communities and freely available fromhttps://github.com/zellerlab/cayman.
Pathways controlling neurotoxicity and proteostasis in mitochondrial complex I deficiency
Authors: Nithianadam Vanitha , Sarkar Souvarish , Feany Mel B. 
Published: January 2024

AbstractNeuromuscular disorders caused by dysfunction of the mitochondrial respiratory chain are common, severe and untreatable. We recovered a number of mitochondrial genes, including electron transport chain components, in a large forward genetic screen for mutations causing age-related neurodegeneration in the context of proteostasis dysfunction. We created a model of complex I deficiency in theDrosophilaretina to probe the role of protein degradation abnormalities in mitochondrial encephalomyopathies. Using our genetic model, we found that complex I deficiency regulates both the ubiquitin/proteasome and autophagy/lysosome arms of the proteostasis machinery. We further performed an in vivo kinome screen to uncover new and potentially druggable mechanisms contributing to complex I related neurodegeneration and proteostasis failure. Reduction of RIOK kinases and the innate immune signaling kinase pelle prevented neurodegeneration in complex I deficiency animals. Genetically targeting oxidative stress, but not RIOK1 or pelle knockdown, normalized proteostasis markers. Our findings outline distinct pathways controlling neurodegeneration and protein degradation in complex I deficiency and introduce an experimentally facile model in which to study these debilitating and currently treatment-refractory disorders.
Testis Specific Serine Kinase 6 (TSSK6) is abnormally expressed in colorectal cancer and promotes oncogenic behaviors
Authors: Delgado Magdalena , Gallegos Zachary , Stippec Steve , McGlynn Kathleen , Cobb Melanie H. , Whitehurst Angelique W. 
Published: January 2024

AbstractCancer testis antigens (CTAs) are a collection of proteins whose expression is normally restricted to the gamete, but abnormally activated in a wide variety of tumors. The CTA, Testis specific serine kinase 6 (TSSK6), is essential for male fertility in mice. Functional relevance of TSSK6 to cancer, if any, has not previously been investigated. Here we find that TSSK6 is frequently anomalously expressed in colorectal cancer and patients with elevated TSSK6 expression have reduced relapse free survival. Depletion of TSSK6 from colorectal cancer cells attenuates anchorage independent growth, invasion and growth in vivo. Conversely, overexpression of TSSK6 enhances anchorage independence and invasion in vitro as well as in vivo tumor growth. Notably, ectopic expression of TSSK6 in semi-transformed human colonic epithelial cells is sufficient to confer anchorage independence and enhance invasion. In somatic cells, TSSK6 co-localizes with and enhances the formation of paxillin and tensin positive foci at the cell periphery, suggesting a function in focal adhesion formation. Importantly, TSSK6 kinase activity is essential to induce these tumorigenic behaviors. Our findings establish that TSSK6 exhibits oncogenic activity when abnormally expressed in colorectal cancer cells. Thus, TSSK6 is a previously unrecognized intervention target for therapy, which could exhibit an exceptionally broad therapeutic window.
ImagingGiardia intestinaliscellular organisation using expansion microscopy revealed atypical centrin localisation
Authors: Soukup J. , Zelená M. , Weisz F. , Kostelanská M. , Nohýnková E. , Tůmová P. 
Published: January 2024

AbstractAdvanced imaging of microorganisms, including protists, is challenging due to their small size. Specimen expansion prior to imaging is thus beneficial to increase resolution and cellular details. Here, we present a sample preparation workflow for improved observations of the single-celled eukaryotic pathogenGiardia intestinalis(Excavata, Metamonada). The binucleated trophozoites colonize the small intestine of humans and animals and cause a diarrhoeal disease. Their remarkable morphology includes two nuclei and a pronounced microtubular cytoskeleton enabling cell motility, attachment and proliferation. By use of expansion and confocal microscopy, we resolved in a great detail subcellular structures and organelles of the parasite cell. The acquired spatial resolution of 43 nm enabled novel observations of centrin localisation atGiardiabasal bodies. Interestingly, non-luminal centrin localization between theGiardiabasal bodies was observed, which is an atypical eukaryotic arrangement. Our protocol includes antibody staining and can be used for the localisation of epitope-tagged proteins, as well as for differential organelle labelling by amino reactive esters. This fast and simple protocol is suitable for routine use without a superresolution microscopy equipment.
Loss of STIM1 and STIM2 in salivary glands disrupts ANO1 function but does not induce Sjogren’s disease
Authors: Son Ga-Yeon , Zou Anna , Wahl Amanda , Huang Kai Ting , Vinu Manikandan , Zorgit Saruul , Zhou Fang , Wagner Larry , Idaghdour Youssef , Yule David I. , Feske Stefan , Lacruz Rodrigo S. 
Published: January 2024

AbstractSjogren’s disease (SjD) is an autoimmune disease characterized by xerostomia (dry mouth), lymphocytic infiltration into salivary glands and the presence of SSA and SSB autoantibodies. Xerostomia is caused by hypofunction of the salivary glands and has been involved in the development of SjD. Saliva production is regulated by parasympathetic input into the glands initiating intracellular Ca2+signals that activate the store operated Ca2+entry (SOCE) pathway eliciting sustained Ca2+influx. SOCE is mediated by the STIM1 and STIM2 proteins and the ORAI1 Ca2+channel. However, there are no studies on the effects of lack of STIM1/2 function in salivary acini in animal models and its impact on SjD. Here we report that male and female mice lackingStim1andStim2(Stim1/2K14Cre) in salivary glands showed reduced intracellular Ca2+levels via SOCE in parotid acini and hyposalivate upon pilocarpine stimulation. Bulk RNASeq of the parotid glands ofStim1/2K14Cremice showed a decrease in the expression ofStim1/2but no other Ca2+associated genes mediating saliva fluid secretion. SOCE was however functionally required for the activation of the Ca2+activated chloride channel ANO1. Despite hyposalivation, ageingStim1/2K14Cremice showed no evidence of lymphocytic infiltration in the glands or elevated levels of SSA or SSB autoantibodies in the serum, which may be linked to the downregulation of the toll-like receptor 8 (Tlr8). By contrast, salivary gland biopsies of SjD patients showed increasedSTIM1andTLR8expression, and induction of SOCE in a salivary gland cell line increased the expression ofTLR8. Our data demonstrate that SOCE is an important activator of ANO1 function and saliva fluid secretion in salivary glands. They also provide a novel link between SOCE and TLR8 signaling which may explain why loss of SOCE does not result in SjD.
Factors influencing the adoption of Internet of Things (IoT) in university libraries: a systematic literature review (SLR)
Authors: Shahzad Khurram , Khan Shakeel Ahmad , Iqbal Abid 
Published: January 2024

Purpose
For the provision of smart library services to end users, tools of the Internet of Things (IoT) play a significant role. The study aims to discover the factors influencing the adoption of IoT in university libraries, investigate the impact of IoT on university library services and identify challenges to adopt IoT applications in university libraries.

Design/methodology/approach
A systematic literature review was carried out to address the objectives of the study. The 40 most relevant research papers published in the world’s leading digital databases were selected to conduct the study.

Findings
The findings illustrated that rapid growth in technology, perceived benefits, the networked world and the changing landscape of librarianship positively influenced the adoption of IoT in university libraries. The study also displayed that IoT supported library professionals to initiate smart library services, assisted in service efficiency, offered context-based library services, provided tracking facilities and delivered effective management of library systems. Results also revealed that a lack of technical infrastructure, security and privacy concerns, a lack of technological skills and unavailability of policy and strategic planning caused barriers to the successful adoption of IoT applications in university libraries.

Originality/value
The study has provided theoretical implications through a valuable addition to the current literature. It has also offered managerial implications for policymakers to construct productive policies for the implementation of IoT applications in university libraries for the attainment of fruitful outcomes. Finally, the study provides a baseline for understanding the adoption of IoT in academic libraries.
Understanding brand reputation: a case study of Chinese wineries
Authors: Shi Fengxia , Gu Qiushi , Zhou Ting 
Published: January 2024

Purpose
Exploring the determinants of a winery brand reputation (BR) and how those determinants interact is vital for the sustainable development of wineries as well as the growth of the wine industry as a whole. This study aims to test an integrated model to better understand the observed measurement constructs of winery brand reputation, including collective reputation (CR), wine label (WL), expert opinion (EO), social media advertising (SMA) and consumer wine knowledge (CWK).

Design/methodology/approach
In-depth interviews, an expert panel review and a pilot study were conducted to examine and improve the observed variables. A questionnaire survey was conducted as the main data source for the study. A total of 616 valid questionnaire responses were collected from 102 cities in mainland China and Hong Kong, Macao and Taiwan from December 2021 to April 2022. Structural equation modeling was conducted for the data analysis.

Findings
This study supported 9 of the 18 proposed theoretical hypotheses. WL, EO and SMA had positive effects on BR. CWK was found to have a moderating effect on the relationship between expert opinions/social media advertising and brand reputation.

Research limitations/implications
The results of this study can guide wine practitioners, researchers and administrators in brand development, label regulation and consumer education.

Originality/value
To the best of the authors’ knowledge, this is the first attempt to examine the determinants of winery brand reputation among Chinese wine consumers. This study explains the mechanism of winery brand reputation, demonstrating the dynamics and effects of the observed measurement constructs on brand reputation.
Assessing students’ sustainability consciousness in relation to their perceived teaching styles: an exploratory study in Pakistani context
Authors: Nousheen Ayesha , Tabassum Farkhanda 
Published: January 2024

Purpose
This study aims to asses students’ sustainability consciousness (SC) in relation to their perceived teaching styles in seven public sector institutions in Pakistan.

Design/methodology/approach
A survey technique was used to collect data from respondents. Grasha’s (1996) Teaching Styles Inventory and Gericke et al.’s (2019) Sustainability Consciousness Questionnaire were used to collect data pertinent to teachers’ teaching styles and students’ SC, respectively. This study’s population was 1,986 students studying in seven educational institutions. A sample of 993 students was selected for the study. Out of the 993 questionnaires distributed, only 753 respondents returned the questionnaire completely filled, resulting in a response rate of 75.83%. Descriptive statistics and structural equation modeling (SEM) were used to analyze the data.

Findings
The results show that students’ mean scores on environmental and social dimensions were higher compared with the economic dimension. Similarly, students’ scores were relatively higher on the knowledge and attitude dimension while lower on the behavior dimension. Moreover, the expert and formal authority teaching styles were the most prevalent teaching styles. Furthermore, SEM results show that various teaching styles affected students’ knowledge and attitude; however, only the delegator teaching style affects all three dimensions of SC.

Research limitations/implications
This research has implications for educational institutions and policymakers to ensure dedicated efforts to promote and integrate education for sustainable development into the educational system and achieve sustainability goals by 2030.

Practical implications
The study findings will help future teachers to effectively integrate sustainability education into their classrooms.

Originality/value
This research expands the discussion on the effectiveness of various teaching styles on SC in teacher education programs.
Test and analysis of friction torque of double-row angular contact ball bearing under vacuum environment
Authors: Ye Biqing , Zhang Kebiao , Zuo Qiang , Zhang Li , Shan Xiaohang 
Published: January 2024

Purpose
The purpose of this paper is to test and analyze the friction torque of double-row angular contact ball bearings under vacuum or ordinary pressure environment, horizontal or upright installation mode, and different rotational speeds, and to provide theoretical bases for the development of aerospace equipment.

Design/methodology/approach
The experiments were carried out to investigate the effects of vacuum or ordinary pressure environment, horizontal or upright installation mode and different rotational speeds on bearing friction torque. To explore the relationship between working conditions and bearing friction torque, firstly, based on the generation source of friction torque, the test principle was determined, a test system was developed and the reliability of data was verified. Secondly, the friction torque of bearing was tested, and the values under various working conditions were obtained. Finally, this paper compared and discussed the test results.

Findings
The test results show that the friction torque value of vacuum environment horizontal installation condition is the largest at different rotational speeds, and the rotational speed has the most significant influence on the friction torque.

Originality/value
The friction torque test system of double-row angular contact ball bearing under vacuum environment was designed and built. The influence rules of vacuum or ordinary pressure environment, horizontal or upright installation mode and different rotational speeds on bearing friction torque were obtained.

Peer review
The peer review history for this article is available at: http://dx.doi.org/10.1108/ILT-08-2023-0259
Unravelling of moderating effect of progressive education between job demand and burnout
Authors: Rajak Rupesh , Rajak Binod , Kumar Vimal , Mathur Swati 
Published: January 2024

PurposeThis study aims to provide a causal framework for teacher burnout (BO) and work engagement (WE) by examining the factors that contribute to it and evaluating how progressive education (PE) affects teachers' performance in Higher education institutions (HEIs).Design/methodology/approachThis study uses a multi-stage sampling technique with the help of computer random generation data from a selected list of teachers. The survey has two sections; the first consisted of a questionnaire of PE, BO, WE and organizational outcomes and the second contained four items to measure the demographic variables. The researcher contacted 745 teachers and asked them to fill up the questionnaire but the authors received only 498 useable responses.FindingsThe results of the study confirmed that moderating role PE reduces the BO of the teachers of HEIs and increases WE. The job demand-resource (JD-R) model was also validated in the Indian context and the model was found suitable for the Indian sample.Research limitations/implicationsThe study has been conducted to manage BO and teachers' engagement in HEIs and the result suggests that the Management of HEIs should value PE characteristics as a crucial component of the educational process. PE encourages academic engagement among professors and students in HEIs.Originality/valueThe study tests the moderating role of PE with the JD-R and the JD-R model in the higher education system in India, which is rarely tested. The study's integrated approach to BO and WE, which provide insight into both viewpoints and aids in employees' poor health.
Transformation of higher education institutions from rhetoric commitment to a place of lifelong learning organizations: a meta synthesis study
Authors: Assefa Yalalem , Gebremeskel Melaku Mengistu , Moges Bekalu Tadesse , Tilwani Shouket Ahmad 
Published: January 2024

PurposeThe current synthesis study was conducted to locate comprehensive perspectives about the transformation of higher education institutions from being the only places where formal education programs are offered into settings where lifelong learning can be integrated. This demands an inquiry through not only instance investigation but also a more comprehensive evidence upsurge which has great importance in obtaining lessons and drawing conclusions from existing facts to show how higher education institutions can be places where lifelong learning is promoted for the good of both individuals and societal advancement.Design/methodology/approachUsing a meta-synthesis methodology, a comprehensive overview of the current state of knowledge in the area of higher education institutions' role in promoting lifelong learning was synthesized.FindingsThe study identified wide-ranging lifelong learning conceptualizations, potential beneficiaries, learning contents and ways of delivery that can be applied in higher education institutions. Furthermore, the practical challenges, partnership and coordination concerns and policy and reform issues towards promoting lifelong learning were addressed.Originality/valueThis meta-synthesis provides crucial evidence for higher education policymakers and practitioners seeking to guide the transformation of their institutions into settings where lifelong learning is integrated with other forms of educational programs, thereby optimizing individual's professional development and societal progress.
The role of contextual factors in conflict handling strategies in logistics service networks
Authors: Özcan Seda , Sevil Oflaç Bengü 
Published: January 2024

Purpose
In business-to-business interactions, conflicts are inevitable, and conflict-handling strategies that consider different variables improve the decision-making process of actors. This study aims to reveal the role of power and criticality in conflict-handling research in logistics service networks.

Design/methodology/approach
A 2 × 2 between-subject experimental design with four different scenarios was used. One hundred sixty logistics service actors completed an online questionnaire. Data were analyzed with ANOVA and paired sample t-tests using the SPSS 28.0 program.

Findings
In comparative scenarios involving high and low power dynamics, individuals with high power and high criticality situations are more inclined to favor the dominating strategy compared to low-power and low-criticality contexts. However, when faced with specific circumstances characterized by both high power and high criticality, actors tend to prioritize the integrating strategy initially, followed by the dominating and obliging strategies in that order. Notably, the statistical analysis revealed no significant interaction effect between criticality and power concerning the integrating, obliging and dominating conflict-handling strategies.

Originality/value
This study used an experimental approach to investigate criticality and power as contextual elements in determining conflict-handling strategies in an inter-firm environment within logistics service networks. This study is particularly groundbreaking in its knowledge of the relationship among power dynamics, conflict criticality and conflict-handling strategies.