bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variabel Daya Tanggap dan Empati Mempengaruhi Kepuasan Pasien Rawat Jalan pada RSUD H. Abdurrahman Sayoeti Kota Jambi
Authors: Tarmizi Ahmad , Asmas Denny , Yasmin Putri 
Published: November 2023

The main problem is also divided into severalsub-problems or exploration questions about service, videlicet 1) What are the service quality variables conforming of power variables? responsiveness, empathy, and patient satisfaction affect sanitarium case satisfaction. H. Abdurrahman Sayoeti Jambi City, and 2) Which service quality variable has the most dominant effect on patient satisfaction at the Sanitarium. The H. Abdurrahman Sayoeti Regional General Hospital, Jambi City, is a Type D General Hospital belonging to the Regional Government of Jambi City. This sanitarium is located at Jl.K.H Hasan AnangNo. 33Rt. 08 Ex. Olak Kemang Kec. Jambi City Bay Lake. Grounded on Mayor RegulationNo. 42 of 2017 the Jambi City Hospital officially uses the name H. Abdurrahman Sayoeti Hospital, Jambi City. This sanitarium was preliminarily the Olak Kemang Inpatient Health Center which in 2018 was converted into a sanitarium. Responsiveness and empathy variables show a tcount value lesser than table (1,663 and 5,329) or sig> a (0.000 <0.05) therefore H0 is rejected and H1 is accepted, meaning that there's a significant positive effect between responsiveness and empathy on patient satisfaction walk in H. Abdurrahman Sayoeti Hospital, Jambi City. The results of this study indicate that the quality of service conforming of responsiveness ( X1) and empathy ( X2) variables has a significant effect on patient satisfaction. Experimenters' suggestions for RSUD. H. Abdurrahman Sayoeti, Jambi City, should be a hospital nurse H. Abdurrahman Sayoeti Jambi City can maximize services according to the promised time, increase trust and responsiveness to patient complaints, maintain empathy and pay attention to the physical installations of the Sanitarium H. Abdurrahman Sayoeti Jambi City.
Opportunities and Challenges: E-Commerce In Indonesia from Business Legal Perspective
Authors: Azzaprillia Tirza 
Published: November 2023

Tujuan penelitian ini mencoba menjawab peluang dan tantangan e-commerce di Indonesia dari perspektif hukum bisnis. E-commerce telah berkembang pesat di Indonesia dalam beberapa tahun terakhir, memberikan peluang bisnis yang besar bagi pengusaha untuk memasarkan dan menjual produk mereka secara online. Perkembangan ini juga memberikan peluang dan tantangan hukum yang harus diperhatikan oleh para pengusaha. Penelitian ini mencoba menjawab Metode penelitian menggunakan metode yuridis normatif dengan pendekatan deskriptif analitis. Hasil penelitian menunjukkan bahwa peluang e-commerce di Indonesia: potensi pasar yang besar, Pertumbuhan pasar yang tinggi, dan kemudahan akses. Tantangan hukum yang harus dihadapi yakni belum jelasnya regulasi perihal perlindungan konsumen, perlindungan data pribadi, persaingan usaha dan hak kekayaan intelektual, dan pajak. Selain regulasi, kepatuhan hukum dan infrastruktur yang tidak merata menjadi tantangan tersendiri.
Penerapan Kitab Undang Hukum Pidana Dan Kitab Undang Hukum Acara Pidana Dalam Penyelesaian Perkara Pidana Pemilu
Authors: Dahri Muhtar , Sayuti Salman , Chandra Febrian 
Published: November 2023

Simultaneous general elections held by the State of Indonesia to elect the President, Vice President, Members of Parliament, Governor and Regent caused various kinds of problems, one of which was in the form of criminal cases, which subsequently led to polemics in related agencies in terms of determining the forms and placing the articles of election criminal cases and their resolution, Therefore, this study attempts to examine the problem of normative research methods by the method of document overview on the application of the Wetboek Van Strafrecht in the specific provisions of the laws on electoral crimes in electoral laws and regulations and on the resolution of electoral criminal cases that refer to Wetboek Van Strafrecht unless the electoral law provides otherwise, that the application of the provisions on electoral criminal cases and their resolution requires a detailed understanding of the parties involved in the electoral process. Therefore, there is a need for training and guidance for stakeholders namely Gakkumdu, in relation to the Electoral Crimes Ordinance and its resolution procedures; To avoid causing disputes within competent authorities in determining the difference between electoral offenses and common offenses
Effects of black sand on Oreochromis niloticus: insights into the biogeochemical impacts through an experimental study
Authors: Saad Eman , Sayed Alaa El-Din H. 
Published: November 2023

Trace elements such as titanium, zirconium, thorium, and uranium, are found in black sand (BS) after weathering and corrosion. Precious metals are not the only valuable elements in black sand, rare earth elements are also found. The aquatic life in lakes and reservoirs is negatively affected by lithophilic elements such as lithium, uranium, and tin. Accordingly, intensive experiments were conducted on Nile tilapia (Oreochromis niloticus) after exposure to isolated black sand. Blood biomarkers, antioxidant balance, morpho-nuclear erythrocyte’s alterations, and histopathological signs have been investigated after fish exposure for 15 days to a 6.4 g BS/kg diet, 9.6 g BS/kg diet, and 2.4 g BS/kg diet. The blood profile, including platelets and white blood cells, was pronouncedly decreased as a result. Functions of the liver and kidneys were impaired. An increase in serum-antioxidant enzymes such as catalase activities and superoxide dismutase was recorded. Also, exposure to black sand induced cellular and nuclear abnormalities in the erythrocytes. In conclusion, the black sand isolated from the Red sea beach influenced Oreochromis niloticus’s hematology, biochemistry, and antioxidant parameters. Poikilocytosis and RBC nuclear abnormalities were also associated with exposure to black sand. The resulting erosion of rocks and rocks’ access to water forces us to consider the seriousness of climatic change on the aquatic ecosystem.
Adverse effects of type 2 diabetes mellitus on ovarian reserve and pregnancy outcomes during the assisted reproductive technology process
Authors: Qin Xue , Du Junhong , He Ruifen , Li Yi , Zhu Qinying , Li Yaxi , Li Hongli , Liang Xiaolei 
Published: November 2023

ObjectiveTo study the effect of type 2 diabetes mellitus(T2DM)on overall ovarian reserve and pregnancy outcomes during assisted reproductive technology (ART) among childbearing infertile women.DesignRetrospective cohort study.SettingThe Reproductive Medicine Special Hospital, The First Hospital of Lanzhou University, between January 2019 and December 2022.Patient(s)A total of 265 infertile female patients aged 20–45 years who underwent in vitro fertilization-embryo transfer (IVF-ET), intracytoplasmic sperm injection-embryo transfer (ICSI-ET), or rescue intracytoplasmic sperm injection-embryo transfer (RICSI-ET) in the first fresh cycle.Intervention(s)None.Main Outcome Measure(s)Serum Anti-Müllerian Hormone (AMH) levels, clinical pregnancy rate (CPR), live birth rate (LBR), and abortion rate (AR) in the T2DM group and non-T2DM group.Result(s)Patients with T2DM showed statistically decreased levels of AMH compared to the non-T2DM group. During ovarian stimulation, those with T2DM required significantly higher total and initial doses of gonadotropin (GN), although they had fewer retrieved oocytes and worse pregnancy outcomes than the non-T2DM group. Multivariate logistic regression analysis adjusting for confounding factors showed that T2DM alone was an independent risk factor for CPR and LBR (adjusted odds ratio [a OR], 0.458, adjusted 95% confidence interval [CI], 0.235-0.891, P = 0.022; a OR, 0.227, 95% CI, 0.101-0.513, P&lt;0.001; respectively), and the abortion rate in the T2DM group was 3.316 times higher than the non-T2DM group(a OR, 3.316, 95%CI, 1.248-8.811, P = 0.016);ConclusionInfertile patients with T2DM have decreased ovarian reserve, and T2DM has a deleterious impact on clinical pregnancy outcomes during the ART process compared with non-T2DM infertile women.CapsuleInfertile women with T2DM have decreased ovarian reserve and pregnancy outcomes during the assisted reproductive technology process compared with non-T2DM infertile women.
Clinical and histopathological characteristics, diagnosis and treatment, and comorbidities of Bowen’s disease: a retrospective study
Authors: Zhou Cheng , Jiang Bin , Zhang Kaoyuan , Wu Jingwen , Huang Cong , Xu Ning , Ye Tinglu , Chen Bancheng , Yu Bo , Zou Yanfen , Shen Changbing 
Published: November 2023

BackgroundBowen’s disease (BD) is a slow-growing precancerous skin condition, often concurrent with other diseases, with a high misdiagnosis rate. Previous studies show that patients with BD in different populations have differentiated characteristics.Materials and methodsA retrospective study was conducted in a tertiary hospital in Shenzhen, China. Data about demographic information, diagnosis and treatment, clinical and pathological characteristics, and comorbidities of 50 patients with BD were collected and analyzed.ResultsClinical data of onset age and disease course of 43 patients with BD were available, the average onset age of male and female patients are 55.1 (standard deviation (SD) = 15.29) and 58.2 (SD = 15.59) years old, respectively; the average disease course of male and female patients are 25.3 (SD = 28.63) and 33.9 (SD = 49.65) months, respectively. The onset age (p = 0.52) and disease course (p = 0.49) between male and female patients are not significantly different. Interestingly, there is a negative correlation between onset age and disease course (r = −0.245, p = 0.11). The correct rate of clinical diagnosis is relatively low (54.00%); Some patients with BD are misdiagnosed as Bowenoid papulosis (10.00%), actinic keratosis (8.00%), basal cell carcinoma (8.00%), seborrheic keratosis (6.00%), and pigmented naevus (4.00%). Trunk and limbs are the most common distribution sites of BD lesions, and 94.00% patients with BD are treated with surgical resection; 66.00% patients with BD had comorbidities, including skin diseases (48.48%), cardiovascular diseases (39.39%), gastrointestinal diseases (30.30%), respiratory diseases (27.27%), and tumors (18.18%). The most commonly observed histopathological characteristics of BD are squamous-cell hyperplasia (86.00%), disordered maturation with atypical keratinocytes (74.00%), atypical mitoses (60.00%), hyperkeratosis with hypokeratosis (48.00%), dermal inflammatory cell infiltration (36.00%), and koilocytosis (22.00%).ConclusionBD often occurs in middle-aged and elderly people and is easily misdiagnosed. The onset age and disease course of patients with BD are not significantly different between males and females, whereas there is a negative correlation between the onset age and disease course. BD is more likely to occur in trunk and limbs in the Chinese population, and most patients with BD are concurrent with comorbidities.
Gut commensals and their metabolites in health and disease
Authors: Krishnamurthy Hari Krishnan , Pereira Michelle , Bosco Jophi , George Jaimee , Jayaraman Vasanth , Krishna Karthik , Wang Tianhao , Bei Kang , Rajasekaran John J. 
Published: November 2023

Purpose of reviewThis review comprehensively discusses the role of the gut microbiome and its metabolites in health and disease and sheds light on the importance of a holistic approach in assessing the gut.Recent findingsThe gut microbiome consisting of the bacteriome, mycobiome, archaeome, and virome has a profound effect on human health. Gut dysbiosis which is characterized by perturbations in the microbial population not only results in gastrointestinal (GI) symptoms or conditions but can also give rise to extra-GI manifestations. Gut microorganisms also produce metabolites (short-chain fatty acids, trimethylamine, hydrogen sulfide, methane, and so on) that are important for several interkingdom microbial interactions and functions. They also participate in various host metabolic processes. An alteration in the microbial species can affect their respective metabolite concentrations which can have serious health implications. Effective assessment of the gut microbiome and its metabolites is crucial as it can provide insights into one’s overall health.SummaryEmerging evidence highlights the role of the gut microbiome and its metabolites in health and disease. As it is implicated in GI as well as extra-GI symptoms, the gut microbiome plays a crucial role in the overall well-being of the host. Effective assessment of the gut microbiome may provide insights into one’s health status leading to more holistic care.
Mobilization of the blaKPC-14 gene among heterogenous plasmids in extensively drug-resistant hypervirulent Klebsiella pneumoniae
Authors: Wang Lin , Shen Weiyi , Cai Jiachang 
Published: November 2023

IntroductionCeftazidime/avibactam (CZA) is an effective alternative for the treatment of infections caused by KPC-producing carbapenem-resistant Klebsiella pneumoniae (CRKP). However, KPC variants with CZA resistance have been observed in clinical isolates, further limiting the treatment options of clinical use.MethodsIn this study, we isolated three KPC-14-producing CRKP from two patients in intensive care units without CZA therapy. The antimicrobial susceptibility was determined using the broth microdilution method. Three CRKP were subjected to whole-genome sequencing to analyze the phylogenetic relatedness and the carriage of antimicrobial resistance genes and virulence factors. Long-read sequencing was also performed to obtain the complete sequences of the plasmids. The horizontal transfer of the blaKPC-14 gene was evaluated by conjugation experiments.ResultsThree CRKP displayed resistance or reduced susceptibility to ceftazidime/avibactam, colistin, and tigecycline. Single-nucleotide polymorphism (SNP) analysis demonstrated the close phylogenetic distance between these strains. A highly similar IncFII/IncR plasmid encoding blaKPC-14 was shared by three CRKP, with blaKPC-14 located in an NTEKPC-Ib element with the core region of ISKpn27- blaKPC-14-ISKpn6. This structure containing blaKPC-14 was also observed in another tet(A)-carrying plasmid that belonged to an unknown Inc-type in two out of three isolates. The horizontal transferability of these integrated plasmids to Escherichia coli EC600 was confirmed by the cotransmission of tet(A) and blaKPC-14 genes, but the single transfer of blaKPC-14 on the IncFII/IncR plasmid failed. Three CRKP expressed yersiniabactin and carried a hypervirulence plasmid encoding rmpA2 and aerobactin-related genes, and were thus classified as carbapenem-resistant hypervirulent K. pneumoniae (hvKP).DiscussionIn this study, we reported the evolution of a mosaic plasmid encoding the blaKPC-14 gene via mobile elements in extensively drug-resistant hvKP. The blaKPC-14 gene is prone to integrate into other conjugative plasmids via the NTEKPC-Ib element, further facilitating the spread of ceftazidime/avibactam resistance.
Genomic characterization of tigecycline-resistant Escherichia coli and Klebsiella pneumoniae isolates from hospital sewage
Authors: Li Ying , Fu Yu , Qiu Yichuan , Liu Qian , Yin Ming , Zhang Luhua 
Published: November 2023

IntroductionThe tigecycline-resistant Enterobacterales have emerged as a great public concern, and the mobile tet(X) variants and tmexCD-toprJ efflux pump are mainly responsible for the spread of tigecycline resistance. Hospital sewage is considered as an important reservoir of antimicrobial resistance, while tigecycline resistance in this niche is under-researched.MethodsIn this study, five Escherichia coli and six Klebsiella pneumoniae strains were selected from a collection of tigecycline-resistant Enterobacterales for further investigation by antimicrobial susceptibility testing, conjugation, whole-genome sequencing, and bioinformatics analysis.ResultsAll five E. coli strains harbored tet(X4), which was located on different plasmids, including a novel IncC/IncFIA(HI1)/IncHI1A/IncHI1B(R27) hybrid structure. In addition, tet(X4)-bearing plasmids were able to transfer by conjugation and be stabilized in the recipient in the absence of antibiotics. tmexCD1-toprJ1 was identified in two K. pneumoniae (LZSFT39 and LZSRT3) and it was carried by a novel multidrug-resistance transposon, designated Tn7368, on a novel IncR/IncU hybrid plasmid. In addition, we found that two K. pneumoniae (LZSFZT3 and LZSRT3) showed overexpression of efflux genes acrB and oqxB, respectively, which was most likely to be caused by mutations in ramR and oqxR.DiscussionIn conclusion, the findings in this study expand our knowledge of the genetic elements that carry tigecycline resistance genes, which establishes a baseline for investigating the structure diversity and evolutionary trajectories of human, animal, and environmental tigecycline resistomes.
Combined contribution of biochar and introduced AM fungi on lead stability and microbial community in polluted agricultural soil
Authors: Chen Xuedong , Tang Lin , Wu Kongyang , Mo Yifan , Tang Qian , Li Gaojie , Zhu Ying 
Published: November 2023

IntroductionLead (Pb) pollution in agricultural soil has been accelerated by industrial development and human activities, and poses a major threat to agricultural ecosystems. Both biochar and arbuscular mycorrhiza (AM) fungi are considered to play an important role in remediation of Pb contaminated soil.MethodsThe combined remediation effects of introduced AM fungi and biochar on soil properties, Pb availability, microbial community and functional profiles were systematically investigated in unsterilized Pb-polluted agricultural soil.ResultsResults indicated that soil nutrients were significantly improved through the combined application of biochar and introduced AM fungi. The introduced AM fungi combined with biochar prepared at 400°C and 500°C promoted the transformation of Pb to a more stable state with low bioavailability. Moreover, the addition of AM fungi and biochar affected the relative abundances of dominant bacteria and fungi at the phylum and genus levels. Biochar mainly affected soil bacterial community and obviously increased the relative abundance of Actinobacteria and Blastococcus. The interactions between biochar and introduced AM fungi mainly affected fungal community, and increased the abundance of Ascomycota and Botryotrichum. Further, PICRUSt analysis indicated biochar amendment supported stronger bacterial metabolic functional potentials.DiscussionTherefore, the combined application of biochar and Therefore, the combined application of biochar and introduced AM fungi could improve soil nutrients, reduce Pb introduced AM fungi could improve soil nutrients, reduce Pb availability, availability, and show and show a positive effect on a positive effect on indigenous microbial communities and indigenous microbial communities and metabolic functions in metabolic functions in farmland soil.
Migraine and major depression: localizing shared genetic susceptibility in different cell types of the nervous systems
Authors: Androulakis X. Michelle , Yu Xuanxuan , Zhu Xia , Thiam Melinda A. , Cai Guoshuai 
Published: November 2023

BackgroundThere is a bidirectional relationship between migraine and major depression disorder (MDD). They likely share important risk genes associated with different cell types in the central nervous system (CNS) and peripheral nervous system (PNS). Profiling the expression of these genes in specific cell types is critical in understanding the pathophysiology of the relationship between migraine and MDD.MethodsAssociated genes shared by migraine and MDD were identified by consolidating multiple curations of human disease-gene associations. Subsequently, the expression of overlapping genes was profiled and compared across the different cell types in CNS, PNS and neurovascular cells using eight single cell RNA sequencing datasets, including two human CNS datasets, two mouse CNS datasets, one human PNS dataset and three mouse PNS datasets.Results45 shared genes between migraine and MDD were identified. Consistently found in all eight datasets, dopaminergic and serotonergic neurotransmitters were broadly expressed in CNS and PNS cell types. Glutamatergic and endocannabinoid genes were specifically expressed in CNS neurons and astrocytes. Synthesis and/or Release and Binding of Neuropeptides were specifically expressed in PNS peptidergic nociceptor (PEP). Genes related to inflammatory factors and immune responses were specifically expressed in CNS microglia. Among which, IL1B and COMT were highly expressed in CNS microglia cells.ConclusionSingle cell RNA sequencing of the CNS and PNS helps to identify the shared genes between migraine and MDD that are enriched in specific cell types. The findings provide new insight in understanding the underlying mechanism of action for the bidirectional co-morbidity between migraine and MDD.
Circulating plasma miR-23b-3p as a biomarker target for idiopathic Parkinson's disease: comparison with small extracellular vesicle miRNA
Authors: Rai Sanskriti , Bharti Prahalad Singh , Singh Rishabh , Rastogi Simran , Rani Komal , Sharma Vaibhav , Gorai Priya Kumari , Rani Neerja , Verma Bhupendra Kumar , Reddy Thota Jagadeshwar , Modi Gyan Prakash , Inampudi Krishna Kishore , Pandey Hem Chandra , Yadav Sanjay , Rajan Roopa , Nikolajeff Fredrik , Kumar Saroj 
Published: November 2023

BackgroundParkinson's disease (PD) is an increasingly common neurodegenerative condition, which causes movement dysfunction and a broad range of non-motor symptoms. There is no molecular or biochemical diagnosis test for PD. The miRNAs are a class of small non-coding RNAs and are extensively studied owing to their altered expression in pathological states and facile harvesting and analysis techniques.MethodsA total of 48 samples (16 each of PD, aged-matched, and young controls) were recruited. The small extracellular vesicles (sEVs) were isolated and validated using Western blot, transmission electron microscope, and nanoparticle tracking analysis. Small RNA isolation, library preparation, and small RNA sequencing followed by differential expression and targeted prediction of miRNA were performed. The real-time PCR was performed with the targeted miRNA on PD, age-matched, and young healthy control of plasma and plasma-derived sEVs to demonstrate their potential as a diagnostic biomarker.ResultsIn RNA sequencing, we identified 14.89% upregulated (fold change 1.11 to 11.04, p &lt; 0.05) and 16.54% downregulated (fold change −1.04 to −7.28, p &lt; 0.05) miRNAs in PD and controls. Four differentially expressed miRNAs (miR-23b-3p, miR-29a-3p, miR-19b-3p, and miR-150-3p) were selected. The expression of miR-23b-3p was “upregulated” (p = 0.002) in plasma, whereas “downregulated” (p = 0.0284) in plasma-derived sEVs in PD than age-matched controls. The ROC analysis of miR-23b-3p revealed better AUC values in plasma (AUC = 0.8086, p = 0.0029) and plasma-derived sEVs (AUC = 0.7278, p = 0.0483) of PD and age-matched controls.ConclusionWe observed an opposite expression profile of miR-23b-3p in PD and age-matched healthy control in plasma and plasma-derived sEV fractions, where the expression of miR-23b-3p is increased in PD plasma while decreased in plasma-derived sEV fractions. We further observed the different miR-23b-3p expression profiles in young and age-matched healthy control.