bluuebunny/crossref_metadata_2025_split huggingface dataset:
SRRM2 splicing factor modulates cell fate in early development
Authors: Carvalho Silvia , Zea-Redondo Luna , Tang Tsz Ching Chloe , Stachel-Braum Philipp , Miller Duncan , Caldas Paulo , Kukalev Alexander , Diecke Sebastian , Grosswendt Stefanie , Grosso Ana Rita , Pombo Ana 
Published: December 2023

AbstractEmbryo development is an orchestrated process that relies on tight regulation of gene expression to guide cell differentiation and fate decisions. Alternative splicing is modulated during development as an additional layer of regulation to reprogram gene expression patterns. TheSrrm2splicing factor has recently been implicated in developmental disorders and diseases, but its role in early mammalian development remains unexplored. Here, we show thatSrrm2dosage is critical for maintaining embryonic stem cell pluripotency and cell identity.Srrm2heterozygosity promotes loss of stemness, characterized by the coexistence of cells expressing naive and formative pluripotency markers, together with extensive changes in gene expression, including genes regulated by serum- response transcription factor and differentiation-related genes. Depletion ofSrrm2by RNA interference in embryonic stem cells shows that the earliest effects of Srrm2 half-dosage are specific alternative splicing events on a small number of genes, followed by expression changes in metabolism and differentiation-related genes. Our findings unveil molecular and cellular roles ofSrrm2in stemness and lineage commitment, shedding light on the roles of splicing regulators in early embryogenesis, developmental diseases and tumorigenesis.Summary statementThis article emphasizes the importance of splicing regulators in early mammalian development by uncovering roles of SRRM2 splicing factor dosage in pluripotency, providing novel insights for a better understanding of Srrm2-related diseases.
SARS-CoV-2 and other coronaviruses in rats, Berlin, Germany, 2023
Authors: Wernike Kerstin , Mehl Calvin , Aebischer Andrea , Heising Mario , Ulrich Rainer G. , Beer Martin 
Published: December 2023

AbstractWe tested 130 rats trapped in Berlin for coronaviruses. Antibodies against SARS-CoV-2 were detected in a single animal only, but not in further 66 rats from the same location, speaking against virus circulation in the rat population. All animals tested negative for SARS-CoV-2 by RT-PCR. However, rodent-associated alphacoronaviruses were found.
A genome wide CRISPR screen reveals that HOXA9 promotes Enzalutamide resistance in prostate cancer
Authors: Roes Michael V. , Dick Frederick A. 
Published: December 2023

AbstractAndrogen receptor inhibitors are commonly used for prostate cancer treatment, but acquired resistance is a significant problem. Co-deletion of RB and p53 is common in castration resistant prostate cancers, however they are difficult to target pharmacologically. To comprehensively identify gene loss events that contribute to enzalutamide response, we performed a genome-wide CRISPR knockout screen in LNCaP prostate cancer cells. This revealed novel genes implicated in resistance that are largely unstudied. Gene loss events that confer enzalutamide sensitivity are enriched for GSEA categories related to stem cell and epigenetic regulation. We investigated the myeloid lineage stem cell factor HOXA9 as a candidate gene whose loss promotes sensitivity to enzalutamide. Cancer genomic data reveals that HOXA9 overexpression correlates with poor prognosis and characteristics of advanced prostate cancer. In cell culture, HOXA9 depletion sensitizes cells to enzalutamide, whereas overexpression drives enzalutamide resistance. Combination of the HOXA9 inhibitor DB818 with enzalutamide demonstrates synergy. This demonstrates the utility of our CRISPR screen data in discovering new approaches for treating enzalutamide resistant prostate cancer.
Spatial, temporal and numerical regulation of polar flagella assembly inPseudomonas putida
Authors: Pulido-Sánchez Marta , Leal-Morales Antonio , López-Sánchez Aroa , Cava Felipe , Govantes Fernando 
Published: December 2023

ABSTRACTThe Gram-negative bacteriumPseudomonas putidabears a tuft of flagella at a single cell pole. New flagella must be assembledde novoevery cell cycle to secure motility of both daughter cells. Here we show that the coordinated action of FimV, FlhF and FleN sets the location, timing and number of flagella assembled. The polar landmark proteins FimV and FlhF are independently targeted to the nascent new pole during or shortly after cell division, but FimV stabilizes FlhF association with the cell poles. FlhF determines the polar position of the flagella by targeting early flagellar components to the cell pole and preventing their nucleation at non-polar sites. FlhF also promotes efficient flagellar assembly and indirectly stimulates Class III flagellar promoter activation by promoting secretion of the anti-FliA anti-σ factor FlgM. The MinD-like ATPase FleN partitions between the cell poles and the cytoplasm. Cytoplasmic FleN regulates flagellar number by preventing excessive accumulation of FlhF at the cell poles that may otherwise lead to hyperflagellation, likely by antagonizing FleQ-dependent transcriptional activation. FimV is essential to FleN polar location. FimV and FleN temporally regulate the onset of flagellar assembly by preventing premature polar targeting of FlhF and the ensuing premature targeting of additional flagellar components. Our results shed new light on the mechanisms that ensure the timely assembly of the appropriate number of flagella at the correct polar location in polarly flagellated bacteria.HIGHLIGHTSFimV, FlhF and FleN determine the position, number and timing of flagellar assemblyFimV is essential to the normal intracellular distribution of FlhF and FleNFlhF restricts flagellar location to the cell poles and promotes efficient assemblySoluble, cytoplasmic FleN prevents polar FlhF accumulation and hyperflagellationPole-bound FimV and FleN prevent premature FlhF recruitment and flagellar assemblyGRAPHICAL ABSTRACT
A Fungal Spore Calendar for England: Analysis of 13 years of Daily Concentrations at Leicester, UK
Authors: Symon F. A. , Anees-Hill S. , Satchwell J. , Fairs A. , Edwards R. , Wardlaw A. J. , Cuthbertson L. , Hansell A. , Pashley C. H. 
Published: December 2023

AbstractBackgroundFungal respiratory allergy is believed to affect up to 30% of hayfever sufferers and up to 70% of severe asthmatics in the UK, however trends in fungal spore seasonality are not well described. Information about seasonal trends would help allergists determine sources of fungal sensitisation and aid disease management.MethodDaily monitoring was carried out at Leicester from 2007 to 2020 using a Burkard volumetric spore trap. Fungal spore concentrations were analysed by microscopy, identifying 23 morphologically distinct taxa. Daily average concentrations were calculated as spores/m3of air sampled and a 90% method used to determine the spore seasons.ResultsThirteen years of data were used to develop a fungal spore calendar for the nine most abundant spore types identified;Alternaria, Cladosporium, Didymella, Leptosphaeria, Sporobolomyces, TilletiopsisandUstilagoplus the wider groupings ofAspergillus/Penicilliumtype and coloured basidiospores. All have been implicated in fungal allergy.We observed long seasons for,Cladosporium, SporobolomycesandTilletiopsis, beginning in late spring and ending in late autumn. In contrastUstilagoand the highly allergenicAlternariashowed relatively short seasons, spanning summer and early autumn. Temperature and precipitation were the main meteorological factors related to spore concentration with wind speed appearing to have little influence. Over the study period, there was a reducing trend for total spore concentrations, driven by a reduction in “wet weather” spores, in line with a reduction in precipitation. Conversely, the “dry weather” spores ofAlternariaandCladosporiumdemonstrated an increasing trend.ConclusionWe present an aeroallergen calendar to provide readily accessible information to patients, healthcare professionals and pharmaceutical companies on exposure concentrations over the year in central England and potentially more widely across the UK. More research on allergenic thresholds would enhance the clinical usefulness of aeroallergen calendars.
Changes in intra- and interlimb reflexes from hindlimb cutaneous afferents after staggered thoracic lateral hemisections during locomotion in cats
Authors: Mari Stephen , Lecomte Charly G. , Merlet Angèle N. , Audet Johannie , Yassine Sirine , Eddaoui Oussama , Genois Gabriel , Nadeau Charlène , Harnie Jonathan , Rybak Ilya A. , Prilutsky Boris I. , Frigon Alain 
Published: December 2023

AbstractWhen the foot dorsum contacts an obstacle during locomotion, cutaneous afferents signal central circuits to coordinate muscle activity in the four limbs. Spinal cord injury disrupts these interactions, impairing balance and interlimb coordination. We evoked cutaneous reflexes by electrically stimulating left and right superficial peroneal nerves before and after two thoracic lateral hemisections placed on opposite sides of the cord at 9-13 weeks interval in seven adult cats (4 males and 3 females). We recorded reflex responses in ten hindlimb and five forelimb muscles bilaterally. After the first (right T5-T6) and second (left T10-T11) hemisections, coordination of the fore- and hindlimbs was altered and/or became less consistent. After the second hemisection, cats required balance assistance to perform quadrupedal locomotion. Short-latency reflex responses in homonymous and crossed hindlimb muscles largely remained unaffected after staggered hemisections. However, mid- and long-latency homonymous and crossed responses in both hindlimbs occurred less frequently after staggered hemisections. In forelimb muscles, homolateral and diagonal mid- and long-latency response occurrence significantly decreased after the first and second hemisections. In all four limbs, however, when present, short-, mid- and long-latency responses maintained their phase-dependent modulation. We also observed reduced durations of short-latency inhibitory homonymous responses in left hindlimb extensors early after the first hemisection and delayed short-latency responses in the right ipsilesional hindlimb after the first hemisection. Therefore, changes in cutaneous reflex responses correlated with impaired balance/stability and interlimb coordination during locomotion after spinal cord injury. Restoring reflex transmission could be used as a biomarker to facilitate locomotor recovery.Key pointsCutaneous afferent inputs coordinate muscle activity in the four limbs during locomotion when the foot dorsum contacts an obstacle.Thoracic spinal cord injury disrupts communication between spinal locomotor centers located at cervical and lumbar levels, impairing balance and limb coordination.We investigated cutaneous reflexes during quadrupedal locomotion by electrically stimulating the superficial peroneal nerve bilaterally, before and after staggered lateral thoracic hemisections of the spinal cord in cats.We showed a loss/reduction of mid- and long-latency responses in all four limbs after staggered hemisections, which correlated with altered coordination of the fore- and hindlimbs and impaired balance.Targeting cutaneous reflex pathways projecting to the four limbs could help develop therapeutic approaches aimed at restoring transmission in ascending and descending spinal pathways.
A comparative study on the corrosion behavior of Q235 steel in saturated acidic red and yellow soils
Authors: Li Yingying , Liu Lanlan , Wang Jun , Xu Song , Su Hui , Xie Yi , Wu Tangqing 
Published: December 2023

Purpose
The purpose of this paper is to study the corrosion behavior of Q235 steel in saturated acidic red and yellow soils.

Design/methodology/approach
The corrosion behavior of Q235 steel in saturated red and yellow soils was compared by weight-loss, SEM/EDS, 3D ultra-depth microscopy and electrochemical measurements.

Findings
Rp of the steel gradually increases and icorr gradually decreases in both the red and yellow soils with time. The Rp of the steel in the red soil is lower, but its icorr is higher than that in the yellow soil. The uniform corrosion rate, diameter and density of the corrosion pit on the steel surface in the red soil are greater than those in the yellow soil. Lower pH, higher contents of corrosive anions and high-valence Fe oxides in the red soil are responsible for its higher corrosion rates and local corrosion susceptibility.

Originality/value
This paper investigates the difference in corrosion behavior of carbon steel in saturated acidic red and yellow soils, which can help to understand the mechanism of soil corrosion.
Analysis of a turbofan engine using a parametric loop
Authors: Dişlitaş Ayşe Nur , Albayrak Çeper Bilge , Yıldız Melih 
Published: December 2023

Purpose
In this study, the performance analysis of the split flow turbofan engine with afterburners has been examined using the parametric cycle analysis method. The purpose of this study is to examine how engine performance is impacted by design parameters and flight ambient values and to develop a software in this context.

Design/methodology/approach
Software has been developed using the open-source PYTHON programming language to perform performance analysis. Mach number, compressor/fan pressure ratio, bypass ratio and density were used as parameters. The effects of these variables on engine performance parameters were investigated.

Findings
Parametric cycle analysis has been calculated for different flight conditions in the range of 0–2 M and 0–15,000 m altitude for turbofan engines. With this study, basic data were obtained to optimize according to targeted flight conditions.

Practical implications
As a result of the performance analysis, the association between the flight conditions and design parameters of engine were determined. A software has been developed that can be used in the design of supersonic gas turbine engines for fast and easy simulation of the design parameters.

Originality/value
The variables used in the literature have been analyzed, and the results of the studies have been incorporated into the developed software, which can be used in innovative engine design. Software is capable to be developed further with the integration of new algorithms and models.
Uncertainty quantification of blade geometric deviation on compressor stability
Authors: Ji Tianyuan , Chu Wuli 
Published: December 2023

Purpose
The geometric parameters of the compressor blade have a noteworthy influence on compressor stability, which should be meticulously designed. However, machining inaccuracies cause the blade geometric parameters to deviate from the ideal design, and the geometric deviation exhibits high randomness. Therefore, the purpose of this study is to quantify the uncertainty and analyze the sensitivity of the impact of blade geometric deviation on compressor stability.

Design/methodology/approach
In this work, the influence of blade geometric deviation is analyzed based on a subsonic compressor rotor stage, and three-dimensional numerical simulations are used to compute samples with different geometric features. A method of combining Halton sequence and non-intrusive polynomial chaos is adopted to carry out uncertainty quantitative analysis. Sobol’ index and Spearman correlation coefficient are used to analysis the sensitivity and correlation between compressor stability and blade geometric deviation, respectively.

Findings
The results show that the compressor stability is most sensitive to the tip clearance deviation, whereas deviations in the leading edge radius, trailing edge radius and chord length have minimal impact on the compressor stability. And, the effects of various blade geometric deviations on the compressor stability are basically independent and linearly superimposed.

Originality/value
This work provided a new approach for uncertainty quantification in compressor stability analysis. The conclusions obtained in this work provide some reference value for the manufacturing and maintenance of rotor blades.
Healthcare industry waste and public health: a systematic review
Authors: Husaini Danladi Chiroma , Bernardez Vinlee , Zetina Naim , Mphuthi David Ditaba 
Published: December 2023

PurposeA direct correlation exists between waste disposal, disease spread and public health. This article systematically reviewed healthcare waste and its implication for public health. This review identified and described the associations and impact of waste disposal on public health.Design/methodology/approachThis paper systematically reviewed the literature on waste disposal and its implications for public health by searching Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA), PubMed, Web of Science, Scopus and ScienceDirect databases. Of a total of 1,583 studies, 59 articles were selected and reviewed.FindingsThe review revealed the spread of infectious diseases and environmental degradation as the most typical implications of improper waste disposal to public health. The impact of waste includes infectious diseases such as cholera, Hepatitis B, respiratory problems, food and metal poisoning, skin infections, and bacteremia, and environmental degradation such as land, water, and air pollution, flooding, drainage obstruction, climate change, and harm to marine and wildlife.Research limitations/implicationsInfectious diseases such as cholera, hepatitis B, respiratory problems, food and metal poisoning, skin infections, bacteremia and environmental degradation such as land, water, and air pollution, flooding, drainage obstruction, climate change, and harm to marine and wildlife are some of the public impacts of improper waste disposal.Originality/valueHealthcare industry waste is a significant waste that can harm the environment and public health if not properly collected, stored, treated, managed and disposed of. There is a need for knowledge and skills applicable to proper healthcare waste disposal and management. Policies must be developed to implement appropriate waste management to prevent public health threats.
Digital transformation in small and medium enterprises: a scientometric analysis
Authors: Parra-Sánchez Diana Teresa , Talero-Sarmiento Leonardo Hernán 
Published: December 2023

PurposeThis paper aims to explore the research field of digital transformation in small and medium enterprises (SMEs), considering the importance of SMEs in the economic development of countries.Design/methodology/approachConsidering the contributions of researchers and the challenges of SMEs to transform their business models, in this paper, the authors conducted a scientometric analysis using CiteSpace that included 448 documents indexed in Scopus.FindingsThe authors appreciated the growth in the number of publications that have studied the digital transformation process in SMEs, showing a niche of researchers interested in the flourishing research topic. Likewise, the authors identified the intention of SMEs to adopt digital technologies such as artificial intelligence, big data, cloud computing, data analytics, electronic commerce and the Internet of Things.Practical implicationsThis paper is a valuable resource for academics and researchers in information systems, decision-makers in digital transformation in SMEs and governmental organisations concerned with digital technologies adoption in SMEs to achieve digital transformation and increase competitiveness and productivity.Originality/valueThis study used CiteSpace to conduct a scientometric analysis to explore how researchers have focused on frameworks and maturity models for measuring SME readiness, the impact of Industry 4.0 on SMEs, guides for helping managers evaluate their Industry 4.0 positioning, the development and implementation of digital business strategies for SMEs, the presentation of cases of SMEs that have driven digital transformation and future research opportunities.
The interplay among paradoxical leadership, industry 4.0 technologies, organisational ambidexterity, strategic flexibility and corporate sustainable performance in manufacturing SMEs of Malaysia
Authors: Hossain Mohammad Imtiaz , Kumar Jeetesh , Islam Md. Tariqul , Valeri Marco 
Published: December 2023

Purpose
Manufacturing firms must embrace smart technologies and develop complex leadership approaches to achieve sustainability. Using the dynamic capability theory, this paper aims to examine the influence of the adoption of industry 4.0 technologies (AT) and paradoxical leadership (PL) on corporate sustainable performance (CSP) of manufacturing small-medium enterprises (SMEs) in Malaysia. Moreover, organisational ambidexterity (OA) is a mediator and strategic flexibility (SF) is a moderator in the study.

Design/methodology/approach
The study is a cross-sectional, quantitative study design that collected 395 usable responses through a simple random sampling technique and a close-ended structured questionnaire. Structural equation modelling (SEM) procedures were followed to analyse the data.

Findings
The statistical outcome implies that the AT significantly influence CSP and OA and mediate with CSP in the presence of OA. Moreover, PL shows a significant impact on OA, is insignificant on CSP and mediates with OA and CSP. The authors found a significant association between OA and CSP; however, SF did not provide evidence of a moderate effect.

Research limitations/implications
The findings of this study clarify the role that organisational capabilities (OA, AT, PL and SF) play in fostering sustainability. The authors suggest incorporating SMEs from different geographies in other sectors by applying diverse methodologies and relevant constructs.

Practical implications
The result injects new perspectives into policy, managerial and individual levels. Installing OA, AT, PL and SF makes SMEs sustainable.

Originality/value
The empirical validation of the influence of OA and AT on CSP and the interaction of PL and SF enriches the organisational and entrepreneurial literature.