bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robust Glycoproteomics Platform Reveals a Tetra‐Antennary Site‐Specific Glycan Capping with Sialyl‐Lewis Antigen for Early Detection of Gastric Cancer
Authors: Liu Luyao , Liu Lei , Wang Yan , Fang Zheng , Bian Yangyang , Zhang Wenyao , Wang Zhongyu , Gao Xianchun , Zhao Changrui , Tian Miaomiao , Liu Xiaoyan , Qin Hongqiang , Guo Zhimou , Liang Xinmiao , Dong Mingming , Nie Yongzhan , Ye Mingliang 
Published: December 2023

AbstractThe lack of efficient biomarkers for the early detection of gastric cancer (GC) contributes to its high mortality rate, so it is crucial to discover novel diagnostic targets for GC. Recent studies have implicated the potential of site‐specific glycans in cancer diagnosis, yet it is challenging to perform highly reproducible and sensitive glycoproteomics analysis on large cohorts of samples. Here, a highly robust N‐glycoproteomics (HRN) platform comprising an automated enrichment method, a stable microflow LC‐MS/MS system, and a sensitive glycopeptide‐spectra‐deciphering tool is developed for large‐scale quantitative N‐glycoproteome analysis. The HRN platform is applied to analyze serum N‐glycoproteomes of 278 subjects from three cohorts to investigate glycosylation changes of GC. It identifies over 20 000 unique site‐specific glycans from discovery and validation cohorts, and determines four site‐specific glycans as biomarker candidates. One candidate has branched tetra‐antennary structure capping with sialyl‐Lewis antigen, and it significantly outperforms serum CEA with AUC values > 0.89 compared against < 0.67 for diagnosing early‐stage GC. The four‐marker panel can provide improved diagnostic performances. Besides, discrimination powers of four candidates are also testified with a verification cohort using PRM strategy. This findings highlight the value of this strong tool in analyzing aberrant site‐specific glycans for cancer detection.
Speciation of Transition Metal Dissolution in Electrolyte from Common Cathode Materials
Authors: Rynearson Leah , Antolini Cali , Jayawardana Chamithri , Yeddala Munaiah , Hayes Dugan , Lucht Brett L. 
Published: December 2023

AbstractSignificant capacity loss has been observed across extended cycling of lithium‐ion batteries cycled to high potential. One of the sources of capacity fade is transition metal dissolution from the cathode active material, ion migration through the electrolyte, and deposition on the solid‐electrolyte interphase on the anode. While much research has been conducted on the oxidation state of the transition metal in the cathode active material or deposited on the anode, there have been limited investigations of the oxidation state of the transition metal ions dissolved in the electrolyte. In this work, X‐ray absorption spectroscopy has been performed on electrolytes extracted from cells built with four different cathode active materials (LiMn2O4 (LMO), LiNi0.5Mn1.5O4 (LNMO), LiNi0.8Mn0.1Co0.1O2 (NMC811), and (x Li2MnO3*(1‐x) LiNiaMnbCocO2, with a+b+c=1) (LMRNMC)) that were cycled at either high or standard potentials to determine the oxidation state of Mn and Ni in solution. Inductively coupled plasma‐mass spectrometry has been performed on the anodes from these cells to determine the concentration of deposited transition metal ions. While transition metal ions were found dissolved in all electrolytes, the oxidation state(s) of Mn and Ni were determined to be dependent on the cathode material and independent of cycling potential.
The synthesis of a secondary branched epoxy‐modified silicone resin and its application at low temperature
Authors: Pan Zhaoqun , Lin Liangwei , Luo Wenhui 
Published: December 2023

AbstractTo enhance the fracture toughness of epoxy resin at low temperature, a secondary branched epoxy‐terminated silicone resin (ESR‐6) was synthesized and incorporated into bisphenol A epoxy resin at different contents. The structure of ESR‐6 was characterized by Fourier transform infrared spectroscopy and nuclear magnetic resonance (1H NMR), and the fracture surface of the composites was observed by scanning electron microscope (SEM) and atomic force microscopy. At room temperature and − 70°C, the maximum values of elongation at break were 15.78% and 12.55% with 10 wt% ESR. Compared with those of neat epoxy resin, the values of elongation at break of the composite were increased by 50.86% and 36.12%. The results of dynamic mechanical analysis also showed that the toughness of the modified resin had been improved. The SEM images of the fracture surfaces suggested that the fracture mode of the modified resin changed from brittle one to plastic one because of the addition of ESR‐6, which further confirmed the toughening effect of ESR‐6. These research results may provide a new strategy for enhancing the low‐temperature toughness of epoxy resins.
A Memristive Oscillator
Authors: Oshima Yugo , Takenobu Taishi , Pu Jiang , Ishiguro Keisuke , Kato Reizo , Yamamoto Hiroshi M. , Kusamoto Tetsuro 
Published: December 2023

AbstractMemristors, or more generally “memristive systems,” are nonlinear electric components that change their resistance depending on a set of state variables. Meanwhile, negative differential resistance (NDR) is an uncommon electrical property that occurs in a few nonlinear electric components. Such a nonlinear property is now widely used in amplifiers and oscillators. It finds that one of the bilayer‐type nickel‐dithiolene complexes shows nonlinear transport with the NDR property and self‐oscillates under a bias current by simply adding a capacitor in parallel. Surprisingly, the Lissajous figure of this molecular material shows a “pinched hysteresis loop” typical of a memristor, and an inductive reactance emerges when a bias current or voltage is applied to the sample. Herein, a new type of oscillator that uses the NDR and hidden inductive properties of a memristive system is reported. The findings suggest that a memristive system can mimic other passive elements, such as an inductor, and can oscillate itself without having them in the circuit. The oscillation mechanism is straightforward and applicable to a wide variety of memristive materials, including resistors with large negative temperature coefficients, discharge tubes, and even nerve cells in the living body.
A short review of the pulsar magnetic inclination angles (II)
Authors: Li Biao‐Peng , Ma Wen‐Qi , Gao Zhi‐Fu 
Published: December 2023

AbstractThe pulsar magnetic inclination angle is a key parameter for pulsar physics. It influences the observable properties of pulsars, such as the pulse beam width, braking index, polarization, and emission geometry. In this study, we give a brief overview of the current state of knowledge and research on this parameter and its implications for the internal physics of pulsars. We use the observed pulsar data of magnetic inclination angle and braking index to constrain the star's number of precession cycles, , which reflects the interaction between superfluid neutrons and other particles inside a neutron star (NS). We apply the method proposed by Cheng et al. (Cheng, Q., Zhang, S. N., Zheng, X. P., & Fan, X. L., 2019, Phys. Rev. D, 99, 083011) to analyze the data of PSR J2013 + 3845 and obtain the constraints for ranging from to . And further analysis suggests that the internal magnetic field structure of PSR J2013 + 3845 is likely dominated by toroidal components. This study may help us understand the process of internal viscous dissipation and the related evolution of the inclination angles of pulsars, and may have important implications for the study of continuous gravitational wave emissions from NS.
Improving the capturing ability of swirl‐based microfluidic chip by introducing baffle wall
Authors: Dang Yanping , Zhang Qin , Ou Zhiming , Hu Shuai 
Published: December 2023

AbstractMicrofluidics technology is promising in developing microparticle manipulation technology due to its nondestructive control and notable adaptability. The manipulation of microparticle based on swirling stagnation point is one of the feasible microfluidics biotechnologies. Aiming to improve the regulation and control of microparticle, baffle wall is introduced into the 2‐microchannel flow field. The theory of wall attachment jet is employed to elucidate the effect of baffle wall. Subsequently, finite volume method simulation is conducted by modeling the swirling flow region (SFR), and the swirling strength is calculated to characterize the SFR's particle‐capturing ability. Experimental validation of the modeling and simulation methods is performed using a printed microfluidic chip, which has demonstrated exceptional reliability. Simulation results show that the baffle wall makes considerable influence on the SFR. Strikingly, a global range adjustment of stagnation point is realized when the baffle wall is configured with a convex shape, which has remarkably outperformed our previous work, where the stagnation point could only move within half range of the field. This work significantly contributes to advanced flow field structure and provides insight into better regulation of stagnation point as well as microparticles. These findings have potential applications in the analysis of the effect of bio/chemical substances on single cell.
Research progress on chemical modifications of tyrosine residues in peptides and proteins
Authors: Zeng Wei , Xue Jianyuan , Geng Haoxing , Liu Xia , Yang Jin , Shen Wei , Yuan Yuqing , Qiang Yujie , Zhu Qing 
Published: December 2023

AbstractThe chemical modifications (CMs) of protein is an important technique in chemical biology, protein‐based therapy, and material science. In recent years, there has been rapid advances in the development of CMs of peptides and proteins, providing new approaches for peptide and protein functionalization, as well as drug discovery. In this review, we highlight the methods for chemically modifying tyrosine (Tyr) residues in different regions, offering a comprehensive exposition of the research content related to Tyr modification. This review summarizes and provides an outlook on Tyr residue modification, aiming to offer readers assistance in the site‐selective modification of macromolecules and to facilitate application research in this field.
Photoluminescence properties of Eu2+‐activated thiogallate phosphors
Authors: Cho Young‐Sik , Bok Moon‐Su , Huh Young‐Duk 
Published: December 2023

AbstractThis work analyzes the crystal structural properties and photoluminescence properties of six Eu2+‐activated thiogallates: CaGa2S4:Eu2+, SrGa2S4:Eu2+, EuGa2S4, BaGa2S4:Eu2+, Sr2Ga2S5:Eu2+, and Eu2Ga2S5. Photoluminescence parameters such as the absorption energy (Eabs), emission energy (Eem), zero‐phonon line energy (E0), Stokes shift (ΔS), and red shift (D) are discussed. This work is the first report on the photoluminescence parameters of these six Eu2+‐activated thiogallates and therefore provides important information about these materials.
Development and validation of a sensitive UPLC–MS/MS analytical method for GFH009 in rat plasma and its application to toxicokinetics studies
Authors: Liu Qiang , Zhao Jin‐Zhu , Zhou Fu‐Sheng , Lan Jiong , Lu Qiang , Ren Hong‐Can 
Published: December 2023

AbstractGFH009 is a potent, highly selective, small molecule that targets and inhibits the activity of the CDK9/cyclin T1 regulatory complex of P‐TEFb. This study aimed to develop and validate a highly selective and sensitive ultrahigh‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for precise quantification of GFH009 in rat plasma. This method was subsequently employed for conducting toxicokinetic studies of GFH009 in rats. Plasma was prepared using a simple protein precipitation method by acetonitrile. Chromatographic separation of the analytes was achieved on a BEH C18 analytical column with a rapid 3.0 min run time and a flow rate of 0.5 ml/min. The calibration curves for plasma samples exhibited excellent linearity over a wide concentration range of 1.0–1,000 ng/ml for GFH009. Intra‐ and inter‐day accuracies were within 92.7–105.7%, and precisions were no more than 6.7%. Furthermore, the analyte demonstrated stability under four different storage conditions, with variations of <15.0%. This study pioneers a methodological innovation by introducing a highly reliable, specific and sensitive analytical method for GFH009 in rat plasma. The successful application of this method in toxicokinetic studies further underscores its significance, offering valuable insights for the methodology of clinical pharmacokinetic research.
UPLC–quadrupole time‐of‐flight–tandem mass spectrometry combined with chemometrics and network pharmacology to differentiate Coreopsis tinctoria Nutt.
Authors: Zhou Xinyu , Cheng Wei , Chen Xinmei , Wang Kaixuan 
Published: December 2023

AbstractCoreopsis tinctoria Nutt. (C. tinctoria) is a traditional medicinal plant, primarily found in plateau areas with altitudes exceeding 3000 m. The efficacy of C. tinctoria appears to be intricately tied to its quality. However, there is a scarcity of studies focused on evaluating the quality of C. tinctoria from diverse geographical locations. In this study, we used ultra‐performance liquid chromatography–quadrupole time‐of‐flight–tandem mass spectrometry to analyze and identify the prevalent chemical components in 12 batches of C. tinctoria sourced from Xinjiang, Qinghai, Tibet, and Yunnan provinces in China. By using cluster analysis and discriminant analysis of partial least squares, we assessed the similarity and identified varying components in the 12 batches of C. tinctoria. Subsequently, their quality was further evaluated. Utilizing network pharmacology, we identified potential active components for the treatment of diabetes mellitus. The findings revealed the presence of 16 flavonoids, 3 phenylpropanes, 2 sugars, 2 amino acids, and 7 hydrocarbons in the analyzed samples. Through variable importance screening, 17 constituents were identified as quality difference markers. Marein and flavanomarein emerged as pivotal markers, crucial for distinguishing variations in C. tinctoria. In addition, network pharmacology predicted 187 targets for 9 common active components, including marein and flavanomarein. Simultaneously, 1747 targets related to diabetes mellitus were identified. The drug–component–disease target network comprised 91 nodes and 179 edges, encompassing 1 drug node, 9 component nodes, and 81 target nodes. In summary, marein and flavanomarein stand out as key biomarkers for assessing the quality of C. tinctoria, offering a scientific foundation for the quality evaluation of C. tinctoria Nutt.
Profiling regulatory T lymphocytes within the tumor microenvironment of breast cancer via radiomics
Authors: Jiang Wenying , Wu Ruoxi , Yang Tao , Yu Shengnan , Xing Wei 
Published: December 2023

AbstractObjectiveTo generate an image‐driven biomarker (Rad_score) to predict tumor‐infiltrating regulatory T lymphocytes (Treg) in breast cancer (BC).MethodsOverall, 928 BC patients were enrolled from the Cancer Genome Atlas (TCGA) for survival analysis; MRI (n = 71 and n = 30 in the training and validation sets, respectively) from the Cancer Imaging Archive (TCIA) were retrieved and subjected to repeat least absolute shrinkage and selection operator for feature reduction. The radiomic scores (rad_score) for Treg infiltration estimation were calculated via support vector machine (SVM) and logistic regression (LR) algorithms, and validated on the remaining patients.ResultsLandmark analysis indicated Treg infiltration was a risk factor for BC patients in the first 5 years and after 10 years of diagnosis (p = 0.007 and 0.018, respectively). Altogether, 108 radiomic features were extracted from MRI images, 4 of which remained for model construction. Areas under curves (AUCs) of the SVM model were 0.744 (95% CI 0.622–0.867) and 0.733 (95% CI 0.535–0.931) for training and validation sets, respectively, while for the LR model, AUCs were 0.771 (95% CI 0.657–0.885) and 0.724 (95% CI 0.522–0.926). The calibration curves indicated good agreement between prediction and true value (p > 0.05), and DCA shows the high clinical utility of the radiomic model. Rad_score was significantly correlated with immune inhibitory genes like CTLA4 and PDCD1.ConclusionsHigh Treg infiltration is a risk factor for patients with BC. The Rad_score formulated on radiomic features is a novel tool to predict Treg abundance in the tumor microenvironment.
Surgical resection of soft tissue metastasis in cancers: A single‐center study of 77 cases over a 7‐year period
Authors: Ye Qingrong , Hu Tu , Sun Zhengwang , Xu Peihang , Wang Chunmeng , Sun Yangbai , Yan Wangjun 
Published: December 2023

AbstractIntroductionSoft tissue metastasis (STM) of cancers, encompassing skeletal muscle and subcutaneous tissue metastasis, is less common due to unique homeostatic conditions. With longer life expectancy and the advent of new imaging modalities, clinical physicians will increasingly encounter and manage such cases. This study retrospectively reviewed cases of STM in visceral cancers who underwent surgery at Fudan University Shanghai Cancer Center over a 7‐year period.MethodsData were collected through a comprehensive review of medical records, including demographic variables, primary tumor characteristics, surgical data, tumor pathology, and outcomes. Survival analysis was performed using Kaplan–Meier curves.ResultsThe study included 77 cases with a median follow‐up period of 854 days. The most common primary tumor sites were the lung (11) and breast (10). The abdominal wall was the most frequent site of metastasis. The combination of visceral metastasis, age over 52 years, and a history of primary tumor correlates with a poorer prognosis. Surgical‐related metastases are associated with a higher degree of differentiation. Additionally, we have identified a better prognosis for patients with cancer of unknown primary (CUP) exhibiting potential resectable soft tissue metastases.ConclusionThe combination of visceral metastasis, age over 52 years, and a history of primary tumor suggest a poorer prognosis. While no significant impact on survival was observed for patients with lymph node metastasis. Surgical‐related metastases are associated with a higher degree of differentiation. CUP patients with potentially resectable soft tissue metastases should be considered for surgical intervention.
Cysteine and glycine‐rich protein 2 retards platelet‐derived growth factor‐BB‐evoked phenotypic transition of airway smooth muscle cells by decreasing YAP/TAZ activity
Authors: Wang Huiyuan , Zhang Yang , Zhong Bo , Geng Yan , Hao Juanjuan , Jin Qiaoyan , Hou Wei 
Published: December 2023

AbstractCysteine and glycine‐rich protein 2 (Csrp2) has emerged as a key factor in controlling the phenotypic modulation of smooth muscle cells. The phenotypic transition of airway smooth muscle cells (ASMCs) is a pivotal step in developing airway remodeling during the onset of asthma. However, whether Csrp2 mediates the phenotypic transition of ASMCs in airway remodeling during asthma onset is undetermined. This work aimed to address the link between Csrp2 and the phenotypic transition of ASMCs evoked by platelet‐derived growth factor (PDGF)‐BB in vitro. The overexpression or silencing of Csrp2 in ASMCs was achieved through adenovirus‐mediated gene transfer. The expression of mRNA was measured by quantitative real‐time‑PCR. Protein levels were determined through Western blot analysis. Cell proliferation was detected by EdU assay and Calcein AM assays. Cell cycle distribution was assessed via fluorescence‐activated cell sorting assay. Cell migration was evaluated using the scratch‐wound assay. The transcriptional activity of Yes‐associated protein (YAP)/transcriptional coactivator with PDZ‐binding motif (TAZ) was measured using the luciferase reporter assay. A decline in Csrp2 level occurred in PDGF‐BB‐stimulated ASMCs. Increasing Csrp2 expression repressed the PDGF‐BB‐evoked proliferation and migration of ASMCs. Moreover, increasing Csrp2 expression impeded the phenotypic change of PDGF‐BB‐stimulated ASMCs from a contractile phenotype into a synthetic/proliferative phenotype. On the contrary, the opposite effects were observed in Csrp2‐silenced ASMCs. The activity of YAP/TAZ was elevated in PDGF‐BB‐stimulated ASMCs, which was weakened by Csrp2 overexpression or enhanced by Csrp2 silencing. The YAP/TAZ activator could reverse Csrp2‐overexpression‐mediated suppression of the PDGF‐BB‐evoked phenotypic switching of ASMCs, while the YAP/TAZ suppressor could dimmish Csrp2‐silencing‐mediated enhancement on PDGF‐BB‐evoked phenotypic switching of ASMCs. In summary, Csrp2 serves as a determinant for the phenotypic switching of ASMCs. Increasing Csrp2 is able to impede PDGF‐BB‐evoked phenotypic change of ASMCs from a synthetic phenotype into a synthetic/proliferative phenotype through the effects on YAP/TAZ. This work implies that Csrp2 may be a key player in airway remodeling during the onset of asthma.
The Stereochemical Course of DmdC, an Enzyme Involved in the Degradation of Dimethylsulfoniopropionate
Authors: Chhalodia Anuj K. , Dickschat Jeroen S. 
Published: December 2023

AbstractThe acyl‐CoA dehydrogenase DmdC is involved in the degradation of the marine sulfur metabolite dimethylsulfonio propionate (DMSP) through the demethylation pathway. The stereochemical course of this reaction was investigated through the synthesis of four stereoselectively deuterated substrate surrogates carrying stereoselective deuterations at the α‐ or the β‐carbon. Analysis of the products revealed a specific abstraction of the 2‐pro‐R proton and of the 3‐pro‐S hydride, establishing an anti elimination for the DmdC reaction.
Liquid Crystalline Nanoparticles via Polymerization‐Induced Self‐Assembly: Morphology Evolution and Function Regulation
Authors: Xu Chang , Zheng Ming‐Xin , Wei Yen , Yuan Jin‐Ying 
Published: December 2023

AbstractLiquid crystalline nanoparticles (LC NPs) are a kind of polymer NPs with LC mesogens, which can form special anisotropic morphologies due to the influence of LC ordering. Owing to the stimuli‐responsiveness of the LC blocks, LC NPs show abundant morphology evolution behaviors in response to external regulation. LC NPs have great application potential in nano‐devices, drug delivery, special fibers and other fields. Polymerization‐induced self‐assembly (PISA) method can synthesize LC NPs at high solid content, reducing the harsh demand for reaction solvent of the LC polymers, being a better choice for large‐scale production. In this review, we introduced recent research progress of PISA‐LC NPs by dividing them into several parts according to the LC mesogen, and discussed the improvement of experimental conditions and the potential application of these polymers.