bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mechanism of action and experimental verification of Gan-song Yin on renal clear cell carcinoma based on network pharmacology and bioinformatics
Authors: Jiang Wenjie , Yuan Ling , Liu Qian , Li Xiangyang , Yang Yifan , Li Jiaqing , Jiao Taiqiang , Niu Yang , Zhang Lei , Dou Hongli , Nan Yi 
Published: December 2023

Abstract
Background: Gan-song Yin (GSY) is originated from the scripture "Gan-song Pills", a medical work of the Ningxia ethnic minorities, and its treatment of kidney diseases has good results. Its method of treating Renal clear cell carcinoma (KIRC) is still unknown, nevertheless.
Methods: Firstly, utilizing a network pharmacology strategy to screen GSY for active components and targets and looking up KIRC-related targets in GeneCards and GEO databases. Secondly, protein interaction networks were constructed and analyzed for GO and KEGG enrichment. Molecular docking was then performed and clinical and other correlations of the network pharmacology results were analyzed using bioinformatic analysis methods. Finally, we performed in vitro cellular experiments with 786-O cells and ACHN cells to validate the results of network pharmacology and bioinformatic analysis.
Results: With the help of network pharmacological analysis, six hub targets were eliminated. Bioinformatics study revealed that the hub targets has clinically significant clinical guiding importance. The results showed that GSY inhibited the proliferation of 786-O cells and ACHN cells, induced cell apoptosis, blocked cell cycle, and reduced cell colony formation ability. qRT-PCR results showed that GSY promoted the expression of ALB and CASP3 genes, and inhibited the expression of EGFR, JUN, MYC and VEGFA genes. Western blot results showed that GSY could promote the expression of ALB and CASP3 protein, and inhibit the expression of EGFR, JUN, MYC and VEGFA protein.
Conclusions: Network pharmacology and bioinformatics analysis showed that GSY could act on multiple targets through a variety of components to achieve the effect of treating KIRC. In this study, we confirmed that GSY inhibits KIRC by regulating the expression of core targets through in vitro cellular experiments, thus providing a reference for subsequent related studies.
The aging and color development of writing in paper archives
Authors: Wang Qiwen , Guo Xiaoshuang , Xu Zhi , Fan Huiming 
Published: December 2023

Abstract

The most important value of archives lies in the handwritten information they record. However, during the storage process, handwriting may change color or fade due to the influence of the storage environment, especially when writing in blue-black ink. Therefore, in response to the phenomenon that files with blue-black ink handwriting are prone to fading, α,α’bipyridine with the ethanol solution was selected as a developer to perform color-developing treatment on naturally aged ink samples to assess the color-developing effect of faded blue-black ink handwriting. The results showed that the artificially aged ink sample had significantly faded. After being treated with a chromogenic agent, the color of the faded ink sample deepened and the outline of the handwriting became clear. The yellow blue color b value changed to -3.15, the residual ink amount increased by 41.74%, and the difference in absorbance curve area decreased by 296.75. Moreover, the chromogenic agent did not cause significant damage to the paper. This result indicates that the chromogenic agent has a good effect on the recovery of naturally aged blue-black ink blots, and this study has certain practical application value for the protection of ancient literature.
In-vivo culture and genetic identification of Sarcoptes scabiei from human isolate using cox1 gene
Authors: Satoto Tri , Dwiputro Alfin Harjuno 
Published: December 2023

Abstract
Scabies is a highly contagious skin disease caused by infestation of Sarcoptes scabiei mite. The host range of S. scabiei is extremely broad. S. scabiei is a skin-infesting ectoparasite of mammals, including human, domestic, and also wild animals. Antigen detection tests could serve as a potential alternative method for diagnosing scabies, but the availability of the antigen hinder further development. The mites could not be cultured using in-vitro media. S. scabiei showed non-specific interaction with the host. Cytochrome c oxidase subunit 1 (cox1 or COI) is a mitochondrial gene which is widely used as molecular marker for classification in animal species, including S. scabiei. Finding showed that S. scabiei isolated from human could infest and cultured in-vivo using rabbit. Rabbit used in the in-vivo culture of the mites showed heavy crust development after 3 weeks of mites infestation. Phylogenetic analysis with maximum-likelihood using cox-1 generates 2 branches, with the value of 74% and 45%. S. scabiei harvested from in-vivo culture showed genetic relation with S. scabiei isolated from different host, including human, dog, and rabbit from different geographic area. Our present finding showed S. scabiei isolated from human could be cultured in rabbit, thus provides alternative source of antigen for further development of scabies diagnostic test.
Comparison of Optimization Approaches on Linear Quadratic Regulator Design for Trajectory Tracking of a Quadrotor
Authors: Ata Baris , Gencal Mashar 
Published: December 2023

Abstract
Linear Quadratic Regulator (LQR) is one of the most prevalent methods used in the control of unmanned aerial vehicles. LQR controllers are commonly employed in the control of both linear and non-linear systems due to their advantages such as easy-to-apply and high-performance structure. However, there is one main difficulty that plays a significant role in the manner of determining the gain for the control signal: choosing appropriate weighting matrices. The selection of these matrices that directly affect the controller performance is generally performed by trial and error, which is laborious and time-consuming. Accordingly, various optimization algorithms have been utilized to determine the weighting matrices of the LQR controller. In this paper, the weighting matrices of the designed LQR controller were obtained using Standard Genetic Algorithm, Differential Evolution, Particle Swarm Optimization, and Grey Wolf Optimization algorithms. The obtained weighting matrices of the LQR controller were tested on an unmanned aerial vehicle simulation, and the performance of optimization algorithms were presented comparatively.
Cytokeratin Based Assessment of Tumour Budding in Colorectal Cancer
Authors: Muthukumarappan Kumudavalli , Narla Swetha , Subramanyan Annapurneswari 
Published: December 2023

Abstract
Background: One of the most prevalent and major causes of cancer-related mortality is colorectal cancer(CRC). Unfortunately, despite being labelled as low-risk cancers based on their TNM stage, many tumours behave poorly. Therefore, a key area of research has been the search for additional prognostic factors in the evaluation of CRC. The independent forecasting tool for colorectal cancer(CRC), particularly in node-negative illness, is tumour budding(TB). The term "tumour buds"(TB) refers to detached (epithelial) tumour cells, which are single or clusters (less than or equal to five cells) at the maximum invasive front. This prospective study was carried out by evaluating tumour buds using pan-cytokeratin immunostain and comparing it with Haematoxylin & Eosin(H&E) staining and clinicopathological parameters.
Methods: Resected specimens without presurgical therapy were analysed in the Department of Pathology for one year. Among histologically proven colorectal carcinoma, TB scores on H & E and pan-cytokeratin immunostained slides were compared.
Results: Of the clinicopathological parameters evaluated in 83 cases of colorectal cancer, there was a strong correlation of tumour budding score with tumour type ('p' value <0.004), grade of the tumour ('p' value <0.001), and metastasis ('p' value <0.001) and were statistically significant. There is a definitive chance of upgradation of TB score by cytokeratin immunostain
Conclusion: TB is a negative prognostic factor responsible for adverse outcomes in CRC patients. Regardless of the scoring method used, cytokeratin-based assessment of tumour budding is superior compared with the TB score assessed by H&E alone.
A Mendelian randomization study confirmed a causal relationship between high basal metabolic rate levels and increased risk of lung cancer
Authors: Yu Zhuchen , Huang Qianwen , Zou Juntao , Xu Fei 
Published: December 2023

Abstract
Purpose We conducted a two-sample Mendelian randomization (MR) study and performed a reverse causation test to assess the potential causal relationship between basal metabolic rate (BMR) and lung cancer, intending to determine whether genetically predicted BMR levels are a causal factor for lung cancer.Methods We collected data on single nucleotide polymorphisms (SNPs) related to basal metabolic rate (BMR) and lung cancer throughout the whole genome. A total of 599 strongly associated SNPs were selected as proxies for BMR to assess the causal relationship between BMR levels and increased risk of lung cancer. Stratified analyses were performed on different histological types of lung cancer to explore the causal relationship between BMR and non-small cell lung cancer/small cell lung cancer risk. The statistical effect was calculated using the Inverse Variance-Weighted(IVW)method, and sensitivity analysis was conducted to assess pleiotropy and heterogeneity.Result The IVW method determined a potential causal relationship between high BMR levels and lung cancer (OR = 1.23, 95%CI = 1.06–1.43, p < 0.01), while dismissing a reverse causal relationship (OR = 1.00, 95%CI = 0.98–1.01, p = 0.63). The causal relationship between high BMR levels and NSCLC was also confirmed (IVW: OR = 1.32, 95%CI = 1.01–1.74, p < 0.05). Further sensitivity analysis validated the stability of these results.Conclusion Our study results provide the first evidence of a causal relationship between high BMR levels and the risk of lung cancer. Additionally, a positive association between high BMR levels and NSCLC risk was observed, while no relation was found with SCLC, possibly due to the lack of data.
Economic Resilience and the Metal Industry: A Qualitative Examination of Dhaka's Metal Businesses in Response to Fluctuating Economic Conditions
Authors: Hasan Md Mehedi , Nipa Meherun Nisa , Abtahi Ahanaf Tahmid , Siam Saleh Ahmed Jalal 
Published: December 2023

Abstract
This study delves into the intricate interplay between the economic environment and Metal Industry's profitability and growth. Metal Industry, a prominent steel manufacturing company, operates in a complex global landscape, necessitating a comprehensive understanding of economic factors that influence its trajectory. Through qualitative research methods, including interviews and document analysis, key personnel's perspectives within the company were garnered. The economic environment's role in shaping profitability and growth was scrutinized, uncovering critical insights. The study reveals that economic variables, encompassing inflation, interest rates, and exchange rates, exert a significant impact on production costs, demand dynamics, and pricing strategies. The company's adaptive measures, as illuminated in response to economic trends and events, underscore its resilience and strategic agility. Challenges posed by factors like increased competition, raw material costs, and trade policy shifts were identified, alongside opportunities such as market expansion and sustainability initiatives. Ethical practices were highlighted as integral to the company's approach, manifesting through sustainable production, regulatory compliance, stakeholder engagement, and responsible sourcing. The study's findings provide a multi-dimensional perspective on how Metal Industry navigates economic complexities, proactively responds to challenges, and ethically positions itself for sustainable profitability and growth. The insights garnered contribute to a nuanced comprehension of the dynamic relationship between businesses and the economic landscape.
Persistent epigenetic signals propel a senescence-associated secretory phenotype and trained innate immunity in CD34+ hematopoietic stem cells from diabetic patients
Authors: Vinci Maria Cristina , Costantino Sarah , Damiano Giulia , Rurali Erica , Rinaldi Raffaella , Vigorelli Vera , Sforza Annalisa , Carulli Ermes , Pirola Sergio , Mastroiacovo Giorgio , Raucci Angela , El-Osta Assam , Paneni Francesco , Pompilio Giulio 
Published: December 2023

Abstract
Background:
Diabetes-induced trained immunity contributes to the development of atherosclerosis and its complications. This study aimed to investigate in humans whether epigenetic signals involved in immune cell activation and inflammation are initiated in hematopoietic stem/progenitor cells (HSPCs) and transferred to differentiated progeny.
Methods and results
High glucose (HG)-exposure of cord blood (CB)-derived HSPCs induced a senescent-associated secretory phenotype (SASP) characterized by cell proliferation lowering, ROS production, telomere shortening, up-regulation of p21 and p27genes, upregulation of NFkB-p65 transcription factor and increased secretion of the inflammatory cytokines TNFα and IL6. Chromatin immunoprecipitation assay (ChIP) of p65 promoter revealed that H3K4me1 histone mark accumulation and methyltransferase SetD7 recruitment, along with the reduction of repressive H3K9me3 histone modification, were involved in NFkB-p65 upregulation of HG-HSPCs, as confirmed by increased RNA polymerase II engagement at gene level. The differentiation of HG-HSPCs into myeloid cells generated highly responsive monocytes, mainly composed of intermediate subsets (CD14hiCD16+), that, like the cells from which they derive, were characterized by SASP features and similar epigenetic patterns at the p65 promoter. The clinical relevance of our findings was confirmed in sternal BM-derived HSPCs of T2D patients. In line with our in vitro model, T2D HSPCs were characterized by SASP profile and SETD7 upregulation. Additionally, they generated, after myeloid differentiation, senescent monocytes mainly composed of proinflammatory intermediates (CD14hiCD16+) characterized by H3K4me1 accumulation at NFkB-p65 promoter.
Conclusions
Hyperglycemia induces marked chromatin modifications in HSPCs, which, once transmitted to the cell progeny, contributes to persistent and pathogenic changes in immune cell function and composition.
Epigallocatechin-3-gallate ameliorates lipopolysaccharide induced acute thymus involution in mice via AMPK/Sirt1 pathway
Authors: Su Qing , Yang Shu-ping , Guo Jun-ping , Rong Yi-ren , Sun Yun , Chai Yu-rong 
Published: December 2023

Abstract

The thymus, a site to culture the naïve T lymphocytes, is susceptible to atrophy or involution due to ageing, inflammation and oxidation. Eepigallocatechin-3-gallate (EGCG) has been proven to possess the anti-inflammatory, anti-oxidant and anti-tumor activity. Here, we investigate the effects of EGCG on thymic involution induced by lipopolysaccharide (LPS), an endotoxin derived from gram-negative bacteria. Methodology included in vivo experiment on female kunming mice exposed to LPS and EGCG. Morphological assessment of thymic involution, the immunohistochemical detection, thymocyte subsets analysis by flow cytometry were further carried out to evaluate the potential role of EGCG on thymus. As a result, we found that EGCG alleviated LPS-induced thymic atrophy, increased mitochondrial membrane potential and SOD levels, and decreased MDA and ROS levels. In addition, EGCG pre-supplement restored the ratio of thymocyte subsets, the expression of autoimmune regulator (Aire), sex determining region Y-box2 (Sox2) and nanog homebox (Nanog), and reduced the number of senescent cells and collagen fiber deposition. Western blotting results indicated that EGCG treatment elevated LPS-induced decrease in pAMPK, Sirt1 protein expression. Collectively, EGCG relieved thymus architecture and function damaged by LPS via regulation of AMPK/ Sirt1 signaling pathway. Our findings may provide a new strategy on protection of thymus from involution caused by LPS by using appropriate adjuvant natural agents. EGCG might be considered as a potential agent for the prevention and treatment of thymic involution.
A data-driven quantitative model for predicting floor groundwater inrush risk under deep and thick coal seam mining
Authors: Zhan Hao , Liu Shouqiang , Wu Qiang , Yin Shangxian , Liu Weitao , Zhang Xiaohui 
Published: December 2023

Abstract
With the increase of coal mining depth, water hazards in the coal mine floor occur frequently. The coal production process is faced with complex water inrush mechanism and variable water inrush main control factors, and the uncertainties among the factors make the prediction of floor water inrush more difficult. In this paper, Tangjiahui Coal Mine, a Northwest China typical coalfield, in the Inner Mongolia Autonomous Region is taken as the research object. The prediction index system including aquifer capacity, aquiclude capacity, and geological structure is selected, with seven prediction factors being considered. Secondly, the analytic hierarchy process and entropy weight method are used to calculate the subjective and objective weights. On this basis, two models of comprehensive weight based on AHP-EW improved by game theory and improved variable weight of floor water inrush risk based on the foundation of comprehensive weight are constructed. The predicted results are displayed by using the powerful spatial management and information processing functions of GIS, and the performance of the two models is discussed and compared. By comparing the prediction results with the in-situ water inrush points, it is found that these positions are in the relatively hazardous areas of floor water inrush, which proves that the prediction model has high accuracy. Finally, the prevention measures of floor water inrush are put forward according to the risk zoning results. The research results can provide a scientific theoretical basis for mine water disaster prediction, and it is also conducive to the sustainable utilization of groundwater resources.
Perceived certainty of punishment as a boundary condition between fear of punishment and compliance with coronavirus disease prevention policies
Authors: Kura Kabiru Maitama , Abubakar Ramatu Abdulkareem 
Published: December 2023

Abstract
This study investigates the potential impact of perceived certainty of punishment on the relationship between fear of punishment and adherence to coronavirus disease (COVID-19) prevention policies. By integrating regulatory focus theory with deterrence literature, we argued that individuals have varying perceptions regarding the certainty of punishment. Individuals who believe that the consequences of non-compliance with COVID-19 prevention policies are definite are more inclined to adhere to them compared to those who do not perceive the consequences as certain. To test this hypothesis, we invited 354 students from five universities in northwestern Nigeria to participate in our study. The findings of our study confirmed that individuals were more likely to adhere to COVID-19 prevention measures implemented by universities in Nigeria when they were motivated by the fear of potential consequences. Additionally, our research revealed that the level of certainty regarding punishment plays a crucial role in influencing individuals’ compliance with COVID-19 prevention policies, particularly in relation to their fear of potential consequences. Lastly, we explored the practical implications and potential avenues for future research.
Ethnic inequities in type of breast cancer surgery in Aotearoa/New Zealand - which factors contribute?
Authors: Boyle Leah , Lawrenson Ross , Nosa Vili , Campbell Ian , Tin Sandar Tin 
Published: December 2023

Abstract
Purpose
Aotearoa/New Zealand (NZ) faces ethnic inequities with respect to breast cancer survival and treatment. This study establishes if there are ethnic differences in i. type of surgery and ii. receipt of radiotherapy (RT) following breast conserving surgery (BCS), among women with early-stage breast cancer in NZ.
Methods
This analysis used Te Rēhita Mata Ūtaetae (Breast Cancer Foundation National Register), a prospectively maintained database of breast cancers from 2000–2020. Logistic regression models evaluated ethnic differences in type of surgery (mastectomy or BCS) and receipt of RT with sequential adjustment for potential contributing factors. Subgroup analyses by treatment facility type were undertaken.
Results
Of the 16,228 women included, 74% were NZ European (NZE), 10.3% were Māori, 9.4% were Asian and 6.2% were Pacific. Over one-third of women with BCS-eligible tumours received mastectomy. Asian women were more likely to receive mastectomy than NZE (OR: 1.62; 95% CI: 1.39, 1.90) as were wāhine Māori in the public system (OR: 1.21; 95% CI: 1.02, 1.44) but not in the private system (OR: 0.78; 95% CI: 0.51, 1.21). In women undergoing BCS, compared to NZE, Pacific women overall and wāhine Māori in the private system were respectively 36% and 38% less likely to receive RT (respective OR: 0.64; 95% CI: 0.50, 0.83 and 0.62; 95% CI: 0.39, 0.98).
Conclusion
A significant proportion of women with early-stage breast cancer underwent mastectomy and significant ethnic inequities exist. NZ guidelines require revisiting to facilitate standardized and equitable surgical management of early-stage breast cancer.
Exploring the Engineering Potential of Crushed Burnt Bricks as Coarse Aggregate in Concrete Applications
Authors: J Selwyn Babu , devolla Hemanth 
Published: December 2023

Abstract
Concrete, a fundamental element in contemporary construction, plays a crucial role in infrastructure development, consisting of fine aggregates, coarse aggregates, cement, and water. However, concerns have risen regarding the overuse of natural aggregates, leading to the depletion of traditional construction materials. This study investigates the engineering feasibility of replacing coarse aggregate with crushed burnt bricks in concrete formulations. Various replacement percentages (10%, 20%, 30%, 40%, and 100% by volume) are explored to achieve specified targets in strength and durability. The evaluation includes workability, hardened density, compressive strength, flexural strength, and water penetration depth, maintaining a consistent water/cement ratio of 0.49 and strategically utilizing chemical admixtures to enhance performance.
The results indicate a decrease in slump and flexural strength with increasing replacement percentages, a 3% reduction in hardened density at 30% replacement, and an increased water penetration depth. The concrete mix, designed for M25 grade, undergoes extensive testing, revealing a trade-off between workability and strength beyond a 20% replacement threshold. This research emphasizes the importance of exploring the engineering potential of crushed burnt bricks as a substitute for conventional coarse aggregates, contributing to environmental conservation and reducing reliance on traditional resources.