bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeted inhibition of the HNF1A/SHH axis by triptolide overcomes paclitaxel resistance in non-small cell lung cancer
Authors: Li Ling-bing , Yang Ling-xiao , Liu Lei , Liu Fan-rong , Li Alex H. , Zhu Yi-lin , Wen Hao , Xue Xia , Tian Zhong-xian , Sun Hong , Li Pei-chao , Zhao Xiao-gang 
Published: January 2024

AbstractPaclitaxel resistance is associated with a poor prognosis in non-small cell lung cancer (NSCLC) patients, and currently, there is no promising drug for paclitaxel resistance. In this study, we investigated the molecular mechanisms underlying the chemoresistance in human NSCLC-derived cell lines. We constructed paclitaxel-resistant NSCLC cell lines (A549/PR and H460/PR) by long-term exposure to paclitaxel. We found that triptolide, a diterpenoid epoxide isolated from the Chinese medicinal herb Tripterygium wilfordii Hook F, effectively enhanced the sensitivity of paclitaxel-resistant cells to paclitaxel by reducing ABCB1 expression in vivo and in vitro. Through high-throughput sequencing, we identified the SHH-initiated Hedgehog signaling pathway playing an important role in this process. We demonstrated that triptolide directly bound to HNF1A, one of the transcription factors of SHH, and inhibited HNF1A/SHH expression, ensuing in attenuation of Hedgehog signaling. In NSCLC tumor tissue microarrays and cancer network databases, we found a positive correlation between HNF1A and SHH expression. Our results illuminate a novel molecular mechanism through which triptolide targets and inhibits HNF1A, thereby impeding the activation of the Hedgehog signaling pathway and reducing the expression of ABCB1. This study suggests the potential clinical application of triptolide and provides promising prospects in targeting the HNF1A/SHH pathway as a therapeutic strategy for NSCLC patients with paclitaxel resistance.
Designed allosteric protein logic
Authors: Plaper Tjaša , Merljak Estera , Fink Tina , Satler Tadej , Ljubetič Ajasja , Lainšček Duško , Jazbec Vid , Benčina Mojca , Stevanoska Sintija , Džeroski Sašo , Jerala Roman 
Published: January 2024

AbstractThe regulation of protein function by external or internal signals is one of the key features of living organisms. The ability to directly control the function of a selected protein would represent a valuable tool for regulating biological processes. Here, we present a generally applicable regulation of proteins called INSRTR, based on inserting a peptide into a loop of a target protein that retains its function. We demonstrate the versatility and robustness of coiled-coil-mediated regulation, which enables designs for either inactivation or activation of selected protein functions, and implementation of two-input logic functions with rapid response in mammalian cells. The selection of insertion positions in tested proteins was facilitated by using a predictive machine learning model. We showcase the robustness of the INSRTR strategy on proteins with diverse folds and biological functions, including enzymes, signaling mediators, DNA binders, transcriptional regulators, reporters, and antibody domains implemented as chimeric antigen receptors in T cells. Our findings highlight the potential of INSRTR as a powerful tool for precise control of protein function, advancing our understanding of biological processes and developing biotechnological and therapeutic interventions.
Implementing focused echocardiography and AI-supported analysis in a population-based survey in Lesotho: implications for community-based cardiovascular disease care models
Authors: Firima Emmanuel , Gonzalez Lucia , Manthabiseng Molulela , Bane Matumaole , Lukau Blaise , Leigh Bailah , Kaufmann Beat A. , Weisser Maja , Amstutz Alain , Tromp Jasper , Labhardt Niklaus Daniel , Burkard Thilo 
Published: January 2024

AbstractIn settings where access to expert echocardiography is limited, focused echocardiography, combined with artificial intelligence (AI)-supported analysis, may improve diagnosis and monitoring of left ventricular hypertrophy (LVH). Sixteen nurses/nurse-assistants without prior experience in echocardiography underwent a 2-day hands-on intensive training to learn how to assess parasternal long axis views (PLAX) using an inexpensive hand-held ultrasound device in Lesotho, Southern Africa. Loops were stored on a cloud-drive, analyzed using deep learning algorithms at the University Hospital Basel, and afterwards confirmed by a board-certified cardiologist. The nurses/nurse-assistants obtained 756 echocardiograms. Of the 754 uploaded image files, 628 (83.3%) were evaluable by deep learning algorithms. Of those, results of 514/628 (81.9%) were confirmed by a cardiologist. Of the 126 not evaluable by the AI algorithm, 46 (36.5%) were manually evaluable. Overall, 660 (87.5%) uploaded files were evaluable and confirmed. Following short-term training of nursing cadres, a high proportion of obtained PLAX was evaluable using AI-supported analysis. This could be a basis for AI- and telemedical support in hard-to-reach areas with minimal resources.
Risk of COVID-19 death in adults who received booster COVID-19 vaccinations in England
Authors: Ward Isobel L. , Robertson Chris , Agrawal Utkarsh , Patterson Lynsey , Bradley Declan T. , Shi Ting , de Lusignan Simon , Hobbs F. D. Richard , Sheikh Aziz , Nafilyan Vahé 
Published: January 2024

AbstractThe emergence of the COVID-19 vaccination has been critical in changing the course of the COVID-19 pandemic. To ensure protection remains high in vulnerable groups booster vaccinations in the UK have been targeted based on age and clinical vulnerabilities. We undertook a national retrospective cohort study using data from the 2021 Census linked to electronic health records. We fitted cause-specific Cox models to examine the association between health conditions and the risk of COVID-19 death and all-other-cause death for adults aged 50-100-years in England vaccinated with a booster in autumn 2022. Here we show, having learning disabilities or Down Syndrome (hazard ratio=5.07;95% confidence interval=3.69-6.98), pulmonary hypertension or fibrosis (2.88;2.43-3.40), motor neuron disease, multiple sclerosis, myasthenia or Huntington’s disease (2.94, 1.82-4.74), cancer of blood and bone marrow (3.11;2.72-3.56), Parkinson’s disease (2.74;2.34-3.20), lung or oral cancer (2.57;2.04 to 3.24), dementia (2.64;2.46 to 2.83) or liver cirrhosis (2.65;1.95 to 3.59) was associated with an increased risk of COVID-19 death. Individuals with cancer of the blood or bone marrow, chronic kidney disease, cystic fibrosis, pulmonary hypotension or fibrosis, or rheumatoid arthritis or systemic lupus erythematosus had a significantly higher risk of COVID-19 death relative to other causes of death compared with individuals who did not have diagnoses. Policy makers should continue to priorities vulnerable groups for subsequent COVID-19 booster doses to minimise the risk of COVID-19 death.
Atomic-scale observation of nucleation- and growth-controlled deformation twinning in body-centered cubic nanocrystals
Authors: Zhong Li , Zhang Yin , Wang Xiang , Zhu Ting , Mao Scott X. 
Published: January 2024

AbstractTwinning is an essential mode of plastic deformation for achieving superior strength and ductility in metallic nanostructures. It has been generally believed that twinning-induced plasticity in body-centered cubic (BCC) metals is controlled by twin nucleation, but facilitated by rapid twin growth once the nucleation energy barrier is overcome. By performing in situ atomic-scale transmission electron microscopy straining experiments and atomistic simulations, we find that deformation twinning in BCC Ta nanocrystals larger than 15 nm in diameter proceeds by reluctant twin growth, resulting from slow advancement of twinning partials along the boundaries of finite-sized twin structures. In contrast, reluctant twin growth can be obviated by reducing the nanocrystal diameter to below 15 nm. As a result, the nucleated twin structure penetrates quickly through the cross section of nanocrystals, enabling fast twin growth via facile migration of twin boundaries leading to large uniform plastic deformation. The present work reveals a size-dependent transition in the nucleation- and growth-controlled twinning mechanism in BCC metals, and provides insights for exploiting twinning-induced plasticity and breaking strength-ductility limits in nanostructured BCC metals.
The gut microbiota modulate locomotion via vagus-dependent glucagon-like peptide-1 signaling
Authors: Lai Tzu-Ting , Tsai Yu-Hsuan , Liou Chia-Wei , Fan Ching-Hsiang , Hou Yu-Tian , Yao Tzu-Hsuan , Chuang Hsiao-Li , Wu Wei-Li 
Published: January 2024

AbstractLocomotor activity is an innate behavior that can be triggered by gut-motivated conditions, such as appetite and metabolic condition. Various nutrient-sensing receptors distributed in the vagal terminal in the gut are crucial for signal transduction from the gut to the brain. The levels of gut hormones are closely associated with the colonization status of the gut microbiota, suggesting a complicated interaction among gut bacteria, gut hormones, and the brain. However, the detailed mechanism underlying gut microbiota-mediated endocrine signaling in the modulation of locomotion is still unclear. Herein, we show that broad-spectrum antibiotic cocktail (ABX)-treated mice displayed hypolocomotion and elevated levels of the gut hormone glucagon-like peptide-1 (GLP-1). Blockade of the GLP-1 receptor and subdiaphragmatic vagal transmission rescued the deficient locomotor phenotype in ABX-treated mice. Activation of the GLP-1 receptor and vagal projecting brain regions led to hypolocomotion. Finally, selective antibiotic treatment dramatically increased serum GLP-1 levels and decreased locomotion. Colonizing Lactobacillus reuteri and Bacteroides thetaiotaomicron in microbiota-deficient mice suppressed GLP-1 levels and restored the hypolocomotor phenotype. Our findings identify a mechanism by which specific gut microbes mediate host motor behavior via the enteroendocrine and vagal-dependent neural pathways.
Comprehensive investigation of quantum oscillations in semimetal using an ac composite magnetoelectric technique with ultrahigh sensitivity
Authors: Zhang Long , Wang Tianyang , Zhang Yugang , Liu Shuang , Sun Yuping , Zhou Xiaoyuan , Sun Young , He Mingquan , Wang Aifeng , Luo Xuan , Chai Yisheng 
Published: January 2024

AbstractQuantum oscillation (QO), a physical phenomenon that reflects the characteristics of the Fermi surface and transport fermions, has been extensively observed in metals and semimetals through various approaches, like magnetostriction, magnetization, resistivity, and thermoelectric power. However, only limited oscillation frequencies can be revealed by the aforementioned methods, particularly in semimetals with intricate Fermi pockets and associated magnetic breakdown phenomena. In this paper, we present the application of an ac composite magnetoelectric (ME) technique to measure the QOs of a topological nodal-line semimetal, ZrSiS, which possesses six fundamental QO frequencies. By employing the ME technique with a maximum magnetic field of 13 T and a minimum temperature of 2 K, we are able to capture all the fundamental frequencies and most of the permissible magnetic breakdown frequencies. Remarkably, a series of magnetic breakdown frequencies around 8000 T were revealed even in a magnetic field as low as 7.5 T. These findings highlight the ME technique as an ultrahigh-sensitive tool for studying Dirac Fermions and other topological semimetals with complex Fermi surfaces.
A systematic review and meta-analysis of transdiagnostic cognitive behavioural therapies for emotional disorders
Authors: Schaeuffele Carmen , Meine Laura E. , Schulz Ava , Weber Maxi C. , Moser Angela , Paersch Christina , Recher Dominique , Boettcher Johanna , Renneberg Babette , Flückiger Christoph , Kleim Birgit 
Published: January 2024

AbstractTransdiagnostic cognitive behavioural psychotherapy (TD-CBT) may facilitate the treatment of emotional disorders. Here we investigate short- and long-term efficacy of TD-CBT for emotional disorders in individual, group and internet-based settings in randomized controlled trials (PROSPERO CRD42019141512). Two independent reviewers screened results from PubMed, MEDLINE, PsycINFO, Google Scholar, medRxiv and OSF Preprints published between January 2000 and June 2023, selected studies for inclusion, extracted data and evaluated risk of bias (Cochrane risk-of-bias tool 2.0). Absolute efficacy from pre- to posttreatment and relative efficacy between TD-CBT and control treatments were investigated with random-effects models. Of 56 identified studies, 53 (6,705 participants) were included in the meta-analysis. TD-CBT had larger effects on depression (g = 0.74, 95% CI = 0.57–0.92, P < 0.001) and anxiety (g = 0.77, 95% CI = 0.56–0.97, P < 0.001) than did controls. Across treatment formats, TD-CBT was superior to waitlist and treatment-as-usual. TD-CBT showed comparable effects to disorder-specific CBT and was superior to other active treatments for depression but not for anxiety. Different treatment formats showed comparable effects. TD-CBT was superior to controls at 3, 6 and 12 months but not at 24 months follow-up. Studies were heterogeneous in design and methodological quality. This review and meta-analysis strengthens the evidence for TD-CBT as an efficacious treatment for emotional disorders in different settings.
Chromosome-scale Genome assembly of the critically endangered White-eared Night-Heron (Gorsachius magnificus)
Authors: Zheng Chenqing , Chen Qing , Huang Shiguo , Song Weizhen , Chen Guoling , Lin Hongzhou , Xu Chunsheng , Qian Xiran , Cheng Yachang , Jiang Aiwu , Fan Zhongyong , Liu Yang 
Published: January 2024

AbstractThe White-eared Night-Heron (Gorsachius magnificus, G. magnificus) is a critically endangered heron that is very poorly known and only found in southern China and northern Vietnam, with an estimated population of 250 to 999 mature individuals. However, the lack of a reference genome has hindered the implementation of conservation management efforts. In this study, we present the first high-quality chromosome-scale reference genome, which was assembled by integrating PacBio long-reads sequencing, Illumina paired-end sequencing, and Hi-C technology. The genome has a total length of 1.176 Gb, with a scaffold N50 of 84.77 Mb and a contig N50 of 18.46 Mb. Utilizing Hi-C data, we anchored 99.89% of the scaffold sequences onto 29 pairs of chromosomes. Additionally, we identified 18,062 protein-coding genes in the genome, with 95.00% of which were functionally annotated. Notably, BUSCO assessment confirmed the presence of 97.2% of highly conserved Aves genes within the genome. This chromosome-level genome assembly and annotation will be valuable for future investigating the G. magnificus’s evolutionary adaptation and conservation.
A detailed database of sub-annual Spanish demographic statistics: 2005–2021
Authors: Lledó Josep , Pavía Jose M. 
Published: January 2024

AbstractThe big data revolution has made it possible to collect, transmit and exploit huge amounts of data. The potential this offer for data analysis, however, clashes with the limitations imposed by laws on protection of personal data. This paper details a new database (DEMOSPA0521) made after processing and summarising more than 868 million demographic records from Spain, corresponding to a period of seventeen years (2005–2021). DEMOSPA0521 is composed of fifteen files: a group of (monthly and daily moving averages) datasets derived from population stocks and a collection of (daily, monthly and quarterly) datasets obtained from population, death, migration and birth statistics. The intra-annual distributions were calculated by exploiting both the temporal dimensions of age and calendar. DEMOSPA0521 also includes eleven R-Code files that enables the summary datasets to be derived from the raw microdata. DEMOSPA0521 can be used to confirm established results and employed to answer new research questions.
Co-roosting relationships are consistent across years in a bat maternity group
Authors: Sunga Julia , Humber Jessica , Broders Hugh G. 
Published: January 2024

AbstractLong-lived, group living animals have the potential to form multiyear relationships. In some temperate bat species, maternity groups break apart and rejoin both daily, as females depart to forage and select day roosts to use, and annually, as bats leave for and return from hibernation. Here, we investigated whether bats have persistent social preferences by testing whether relationships between dyads in a focal year could be predicted by previous years. We also hypothesized that experience influences social preferences and predicted that an individual’s age would influence its network position, while familiarity with bats of the same cohort would drive persistent social preferences. We quantified roost co-occurrence in little brown myotis (Myotis lucifugus) in Salmonier Nature Park, Newfoundland, Canada both within and among years. We found that roost co-occurrence patterns of previous years still had predictive value even when accounting for potential roost fidelity. However, we found no evidence that cohort familiarity or age explained any of the variation. Overall, we found long-term patterns of association in this temperate bat species that suggest levels of social complexity akin to other large mammal species.
A clinical study evaluating the combination of LISA and SNIPPV for the treatment of respiratory distress syndrome in preterm infants
Authors: Permall Dhivya Lakshmi , Zhang Yuhan , Li Hanyue , Guan Yafei , Chen Xiaoqing 
Published: January 2024

AbstractTo compare the therapeutic effect of less invasive surfactant administration (LISA) followed by synchronized nasal intermittent positive pressure ventilation (SNIPPV) and traditional intubate-Surfactant-Extubate (InSurE) strategy for the treatment of neonatal respiratory distress syndrome (NRDS). A single-center, non-randomized and single- blinded study Tertiary neonatal intensive care unit 89 infants enrolled were preterm with gestational age < 366/7 weeks and clinically diagnosed with neonatal RDS (NRDS) Interventions: 32 infants were assigned to the LISA + SNIPPV group and 57 infants to the InSurE + nCPAP group. No statistically significant differences were noted in the baseline characteristics of the enrolled infants. A lower proportion of infants developed BPD in the LISA + SNIPPV group compared to the InSurE + CPAP group [10 (31.25%) vs. 21 (36.84%), P > 0.05]; however, there was no statistically significant difference. The number needed to treat (NNT) with LISA + SNIPPV to prevent BPD development is 18. The mortality rate was not significant between our study arms [1 (3.13%) vs 2 (3.51%), P > 0.05]. There were no statistically significant differences in the durations (days) of MV [(12.18 ± 13.89) vs. (11.35 ± 11.61), P > 0.05], oxygen therapy [(35.03 ± 19.13) vs. (39.75 ± 17.91), P > 0.05] and re-intubation rates [(0.19 ± 0.40) vs. (0.21 ± 0.45), P > 0.05] between the two study groups. In terms of complications, the incidence of patent ductus arteriosus (PDA) [24 (75.00%) vs. 27 (47.37%), P < 0.05] was higher and a lower rate of disturbed liver function [1 (3.23%) vs. 19 (33.33%), P < 0.05] were observed in the LISA + SNIPPV group. Acid–base imbalances were reportedly significantly higher in the InSurE group (P < 0.05). No significant differences in other complications were noted. In the interventional group, FiO2 requirements were significantly lower up until the 3rd week of treatment [FiO2 at day 0, (30.75 ± 4.78) vs. (34.66 ± 9.83), P < 0.05; FiO2 at day 21, (25.32 ± 3.74) vs. (29.11 ± 8.17), P < 0.05], as was RSS on days 2 [(0.77 ± 0.38) vs. (1.94 ± 0.75), P < 0.05] and 3 [(0.66 ± 0.33) vs. (1.89 ± 0.82), P < 0.05] after treatment. Additionally, infants in the standard group had a significantly prolonged hospital stay (days) [(45.97 ± 16.93) vs. (54.40 ± 16.26), P < 0.05]. The combination of LISA and SNIPPV for NRDS can potentially lower the rate of BPD, FiO2 demand and shorten the length of hospitalization.
Superconductivity of barium with highest transition temperatures in metallic materials at ambient pressure
Authors: Mito Masaki , Tsuji Hiroki , Tajiri Takayuki , Nakamura Kazuma , Tang Yongpeng , Horita Zenji 
Published: January 2024

AbstractPressure-induced superconductivity often occurs following structural transition under hydrostatic pressure (PHP) but disappears after the pressure is released. In the alkali-earth metal barium, superconductivity appears after structural transformation from body-centered cubic structure to hexagonal-close-packed (hcp) structure at PHP = 5 GPa, and the superconducting transition temperature (Tc) reaches a maximum of 5 K at PHP = 18 GPa. Furthermore, by stabilizing the low-temperature phase at PHP ~ 30 GPa, Tc reached a higher level of 8 K. Herein, we demonstrate a significantly higher Tc superconductivity in Ba even at ambient pressure. This was made possible through severe plastic deformation of high-pressure torsion (HPT). In this HPT-processed Ba, we observed superconductivity at Tc = 3 K and Tc = 24 K in the quasi-stabilized hcp and orthorhombic structures, respectively. In particular, the latter Tc represents the highest value achieved at ambient pressure among single-element superconducting metals, including intermetallics. The phenomenon is attributed to a strained high-pressure phase, stabilized by residual strains generated from lattice defects such as dislocations and grain boundaries. Significantly, the observed Tc far exceeds predictions from DFT calculations under normal hydrostatic compressions. The study demonstrates the importance of utilizing high-pressure strained phases as quasi-stable superconducting states at ambient pressure.
Pathological complete response to neoadjuvant chemotherapy may improve antitumor immune response via reduction of regulatory T cells in muscle-invasive bladder cancer
Authors: Ikarashi Daiki , Kitano Shigehisa , Tsuyukubo Takashi , Yamashita Makiko , Matsuura Tomohiko , Maekawa Shigekatsu , Kato Renpei , Kato Yoichiro , Kanehira Mitsugu , Takata Ryo , Sugai Tamotsu , Obara Wataru 
Published: January 2024

AbstractThe prognosis for patients who achieve a pathologic complete response in bladder cancer is excellent, but the association between their prognosis and the tumor microenvironment is unclear. We investigated the tumor immune microenvironment of those with pathological complete response after platinum-based neoadjuvant chemotherapy for cT2-4aN0M0 bladder cancer using multiplex fluorescence immunohistochemistry. Our retrospective study included 12 patients with pathological complete response who underwent radical cystectomy following neoadjuvant chemotherapy for cT2-4aN0M0 muscle-invasive bladder cancer. We assessed the density of several immune cell types in pretreatment and posttreatment tissues via multiplex fluorescence immunohistochemical analysis. The median age was 67 years; 10 patients were male. Nine (75%) and 3 (25%) patients were cT2 and cT3, respectively. The 5-year progression-free and overall survivals were 90% and 100%, respectively. The densities of regulatory T cells (Treg; CD3+CD4+FoxP3+ cell) were significantly decreased and almost disappeared in the tumor microenvironment of posttreatment tissue compared with pretreatment tissue. Other immune cells, such as effector T cells or M2 macrophages, were not significantly changed between posttreatment and pretreatment tissues. In pathological complete response, Tregs in the tumor immune microenvironment were significantly decreased after platinum-based chemotherapy for muscle-invasive bladder cancer. The temporary arresting of immune response in the tumor microenvironment may reflect a favorable prognosis due to the decrease of Tregs with tumor shrinkage and improve the host tumor immune response.