bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of cold storage techniques to improve mass rearing of Cleruchoides noackae from Thaumastocoris peregrinus eggs
Authors: Rodrigues Angelo Peruffo , Tavares Wagner de Souza , Zanuncio José Cola , Wilcken Carlos Frederico , Foerster Luis Amilton , Barbosa Leonardo Rodrigues 
Published: November 2023

AbstractThe egg parasitoid Cleruchoides noackae Lin & Huber, 2007 (Hymenoptera: Mymaridae) is originated from Australia and the main biological control agent of Thaumastocoris peregrinus Carpenter & Dellapé, 2006 (Hemiptera: Thaumastocoridae) on Eucalyptus L'Hér (Myrtaceae). Companies that grow Eucalyptus are in need of a mass rearing protocol to increase the number of individuals produced and improve the quality of this parasitoid. The aim of this study was to define a protocol for mass rearing C. noackae in T. peregrinus eggs, based in the evaluations of the key biological attributes of this parasitoid in the parental and F1 generations, after the cold storage of the parasitised host eggs. Two methods were tested as C. noackae rearing protocols. In the first, parasitised eggs of T. peregrinus by C. noackae were cold stored for 7 days after being left in a climatic chamber at 24 ± 2°C, 60 ± 10% RH and a photoperiod of 12:12 (light:dark) h (standard environmental conditions) for 3, 6, 9 or 12 days. In the second, T. peregrinus eggs parasitised by C. noackae were maintained in a climatic chamber under standard environmental conditions for 6 days, after which these eggs were cold-stored for 0 (control), 7, 14 or 21 days. Parasitism (%), and the development period (parasitism to adult) and female proportion (%) of C. noackae were evaluated. Based on the results (parental generation: parasitism, around 45%; F1 generation: parasitism, around 55%; development period, around 16 days; female proportion, around 60%), eggs should be stored at 5°C on the sixth day after parasitism by C. noackae and maintained at this temperature for 7 days. The cold storage of T. peregrinus eggs, after parasitism, can be included in the mass rearing protocols of the parasitoid C. noackae.
ARCHIMEDES AT SYRACUSE: TWO NEW WITNESSES TO CASSIUS DIO'S ROMAN HISTORY 15 (TZETZES’ CARMINA ILIACA AND HYPOMNEMA IN S. LVCIAM)
Authors: Rance Philip 
Published: November 2023

AbstractCassius Dio's fragmentary Roman History 15 contains an account of Archimedes’ role in defending Syracuse during the Roman siege of 213–212 b.c., incorporating a legendary tale about a solar reflector Archimedes constructed to burn Roman warships, and including details of his death when the city fell. The textual basis of this famous episode depends on two derivative twelfth-century works: Zonaras’ Epitome of Histories (9.4–5) and Tzetzes’ Chiliades (2.35). After clarifying the present state of enquiry, this paper introduces two new witnesses, overlooked by editors of Dio and extensive scholarship on Archimedes, and assesses their value for reconstructing Dio's text. Comparative analysis of corresponding Dio-derived material in Tzetzes’ Carmina Iliaca and Hypomnema in S. Luciam, especially verbal correspondences with Zonaras’ Epitome, demonstrates that they are independent and, sometimes, superior witnesses to Dio's wording and content, reflecting Tzetzes’ selective use of the Roman History in different verse and prose compositions over several decades. The study considers editorial implications for this section of Dio's work and general characteristics of Tzetzes’ writings as repositories of testimonia and fragments.
The first validated record of Epinephelus areolatus (Forsskål, 1775) (Perciformes: Serranidae) from Syrian marine waters
Authors: Hassan Mohamad , Alchikh Ahmad Alaa 
Published: November 2023

Abstract
Here, we report the first validated record of the non-indigenous grouper species Epinephelus areolatus (Perciformes: Serranidae) along the Mediterranean coast of Syria. A single specimen of E. areolatus (Forsskål, 1775), was found on 29 September 2021 during a recurrent ichthyological survey, in a rocky habitat from the Mediterranean coast of Syria. This represents the first confirmed record of this species, native to the Red Sea, for the Syrian waters and the fourth for the Mediterranean Sea. The present record fills the gap with species distribution along the eastern Mediterranean and provides further evidence that the environmental alterations due to the combination of human activities and climate change render the Mediterranean waters increasingly likely to accommodate tropical species, possibly at the expense of the native ones.
Antimony resistance and gene expression inLeishmania: spotlight on molecular and proteomic aspects
Authors: Madusanka Rajamanthrilage Kasun , Karunaweera Nadira D. , Silva Hermali , Selvapandiyan Angamuthu 
Published: November 2023

AbstractLeishmaniasis is a vector-borne parasitic disease caused byLeishmaniaparasites with a spectrum of clinical manifestations, ranging from skin lesions to severe visceral complications. Treatment of this infection has been extremely challenging with the concurrent emergence of drug resistance. The differential gene expression and the discrepancies in protein functions contribute to the appearance of 2 distinct phenotypes: resistant and sensitive, but the current diagnostic tools fail to differentiate between them. The identification of gene expression patterns and molecular mechanisms coupled with antimony (Sb) resistance can be leveraged to prompt diagnosis and select the most effective treatment methods. The present study attempts to use comparative expression of Sb resistance-associated genes in resistant and sensitiveLeishmania, to disclose their relative abundance in clinical orin vitroselected isolates to gain an understanding of the molecular mechanisms of Sb response/resistance. Data suggest that the analysis of resistance gene expression would verify the Sb resistance or susceptibility only to a certain extent; however, none of the individual expression patterns of the studied genes was diagnostic as a biomarker of Sb response ofLeishmania. The findings highlighted will be useful in bridging the knowledge gap and discovering innovative diagnostic tools and novel therapeutic targets.
“There Is a Corpse in the Room”: On Political Guilt and Reparation of the Past
Authors: Gatta Giunia 
Published: November 2023

AbstractPolitical theory scholarship tends to resist guilt, and especially collective guilt, as a framework for thinking about wrongs committed in the past or still enduring. The voices and experiences of those wronged, however, often imply that they are attributing guilt, and they are attributing it to a collectivity. I follow their lead and think through the potential of political guilt in motivating reparation and redress. Drawing on insights that Karl Jaspers fails to fully develop, I appropriate his notion of political guilt as situation, and read it as something that is contested among victims, perpetrators, and bystanders. Through contestation political guilt creates political spaces for reckoning with the past, and can be instrumental in making space for marginalized voices. I apply my framework to race relations in the contemporary United States, but guilt could be a catalyst to rethink postcolonial relations as well.
A
$dd^c$
-TYPE CONDITION BEYOND THE KÄHLER REALM
Authors: Stelzig Jonas , Wilson Scott O. 
Published: November 2023

AbstractThis paper introduces a generalization of the
$dd^c$
-condition for complex manifolds. Like the
$dd^c$
-condition, it admits a diverse collection of characterizations, and is hereditary under various geometric constructions. Most notably, it is an open property with respect to small deformations. The condition is satisfied by a wide range of complex manifolds, including all compact complex surfaces, and all compact Vaisman manifolds. We show there are computable invariants of a real homotopy type which in many cases prohibit it from containing any complex manifold satisfying such
$dd^c$
-type conditions in low degrees. This gives rise to numerous examples of almost complex manifolds which cannot be homotopy equivalent to any of these complex manifolds.
The potential for using wood mulch for agricultural production
Authors: Richardson Matthew L. , Arlotta Caitlin G. , Lopez Francis B. 
Published: November 2023

Abstract
Making woody mulch (WM) from organic waste is one solution for repurposing waste. Our work had two primary objectives. First, we wanted to destermine the current use of WM as a soil cover, barriers to use, benefits, and possible motivations for adopting the use of WM by home and commercial growers for cultivating crops in Barbados and the Baltimore-Washington, DC metropolitan region in the USA. To accomplish this objective, we administered a survey to growers in both regions. Second, we wanted to determine the benefits of using WM in agricultural production for sweet potatoes (both regions) and Hungarian hot wax peppers (USA). We measured whether WM influenced crop survival, crop yield, crop nutrients, weed mass, and soil characteristics in replicated plots covered with a layer of WM or left bare. Growers reported that expense, availability, and ease of application were barriers to using WM. Despite the barriers, many growers were using, or had previously used, WM and reported myriad benefits, including improving plant yield and/or nutrients, preventing weed growth, maintaining soil moisture and reducing irrigation needs, improving soil fertility, reducing soil erosion, reducing compaction from heavy rain, and maintaining soil temperature. Our data from replicated field trials verified some of the potential benefits reported by growers. WM in some cases promoted higher crop survival and yield of sweet potatoes, suppressed weeds, conserved soil moisture, and maintained higher soil temperature. Understanding which crops benefit from WM and the longer-term effects of WM on crops and soil are deserving of future study.
Persistent Model Biases in the Spatial Variability of Winter North Atlantic Atmospheric Circulation
Authors: Tao Qin , Sjolte Jesper , Muscheler Raimund 
Published: November 2023

AbstractThe three leading modes of the North Atlantic atmospheric circulation explain about 70% of the winter climate variability. Although climate models generally can capture these modes, biases may induce large uncertainties in regional climate predictions. Here, we evaluate the leading winter modes simulated by CMIP5‐PMIP3 and CMIP6‐PMIP4 models from the last millennium to future scenarios in comparison with historical reanalysis and paleo‐reconstructions. The models generally have a good representation of the average spatial pattern of the North Atlantic Oscillation (NAO) while showing a larger spread in performance for the East Atlantic and Scandinavian patterns. In contrast to historical reanalysis, the simulated NAO pattern tends to be rather stationary under various climate states over the years 861–2100. Such underestimated spatial variability in the simulated NAO is directly related to the biased spatial shifts in NAO‐related regional temperature and precipitation changes, inducing uncertainties in climate projections over the North Atlantic sector.
A New Lithospheric Density and Magnetic Susceptibility Model of Iran, Starting From High‐Resolution Seismic Tomography
Authors: Maurizio G. , Braitenberg C. , Sampietro D. , Capponi M. 
Published: November 2023

AbstractWe propose a new model for the crust and upper mantle in Iran by joint inversion of gravity and magnetic fields, constrained with a seismic tomography model. We then calculate shear modulus from the Vs velocities and densities. The crust and mantle tomography model is first converted to a density cube through empirical and petrological velocity‐density relations. The starting susceptibility is assigned to a two‐layer homogeneous model, above a heat flow‐derived Curie depth. Considering the uncertainties in the density‐velocity relations, and the starting layered susceptibility variation, we refine the model by a constrained inversion of the gravity and magnetic fields with a Bayesian approach, producing the final 3D density and susceptibility model. The area is tectonically active with high seismicity and active faulting which are regulated by the crustal density and rigidity variations. Higher rigidity matches lower seismicity and extended deserts and basins, suggesting the control of their development. The Neo‐Tethys suture, extending ∼1,500 km long, as well as the Paleo‐Tethys suture match crustal scale density variations, defining characteristics of the lighter Arabian plate and denser Eurasian crust. The South Caspian Basin is enigmatic, due to focusing on the seismicity along all its borders, but with relatively low average rigidity, which is contrary to what is observed for Iran, where the reduced rigidity correlates with higher seismicity. The 3D density model will be useful for numerical geodynamic models and obtaining geologic inferences from the crustal‐scale units.
USP19 deubiquitinase inactivation regulates α-synuclein ubiquitination and inhibits accumulation of Lewy body-like aggregates in mice
Authors: Schorova Lenka , Bedard Nathalie , Khayachi Anouar , Ho Hung-Hsiang , Bolivar-Pedroso Joao , Huynh Julie , Piccirelli Mikaela , Wang Yifei , Plourde Marie , Luo Wen , del Cid-Pellitero Esther , Shlaifer Irina , Castellanos-Montiel María José , Yu Ziqi , Valenzuela Dulce Valeria Carrillo , Lacalle-Aurioles María , Kriz Anita , Ye Yihong , Durcan Thomas M. , Wing Simon S. 
Published: November 2023

AbstractThe USP19 deubiquitinase is found in a locus associated with Parkinson’s Disease (PD), interacts with chaperonins, and promotes secretion of α-synuclein (α-syn) through the misfolding-associated protein secretion (MAPS) pathway. Since these processes might modulate the processing of α-syn aggregates in PD, we inactivated USP19 (KO) in mice expressing the A53T mutation of α-syn and in whom α-syn preformed fibrils (PFF) had been injected in the striatum. Compared to WT, KO brains showed decreased accumulation of phospho-synuclein (pSyn) positive aggregates. This improvement was associated with less activation of microglia and improved performance in a tail-suspension test. Exposure of primary neurons from WT and KO mice to PFF in vitro also led to decreased accumulation of pSyn aggregates. KO did not affect uptake of PFF nor propagation of aggregates in the cultured neurons. We conclude that USP19 instead modulates intracellular dynamics of aggregates. At an early time following PFF injection when the number of pSyn-positive neurons were similar in WT and KO brains, the KO neurons contained less aggregates. KO brain aggregates stained more intensely with anti-ubiquitin antibodies. Immunoprecipitation of soluble proteins from WT and KO brains with antibodies to pSyn showed higher levels of ubiquitinated oligomeric species in the KO samples. We propose that the improved pathology in USP19 KO brains may arise from decreased formation or enhanced clearance of the more ubiquitinated aggregates and/or enhanced disassembly towards more soluble oligomeric species. USP19 inhibition may represent a novel therapeutic approach that targets the intracellular dynamics of α-syn complexes.
A proteomic meta-analysis refinement of plasma extracellular vesicles
Authors: Vallejo Milene C. , Sarkar Soumyadeep , Elliott Emily C. , Henry Hayden R. , Powell Samantha M. , Diaz Ludovico Ivo , You Youngki , Huang Fei , Payne Samuel H. , Ramanadham Sasanka , Sims Emily K. , Metz Thomas O. , Mirmira Raghavendra G. , Nakayasu Ernesto S. 
Published: November 2023

AbstractExtracellular vesicles play major roles in cell-to-cell communication and are excellent biomarker candidates. However, studying plasma extracellular vesicles is challenging due to contaminants. Here, we performed a proteomics meta-analysis of public data to refine the plasma EV composition by separating EV proteins and contaminants into different clusters. We obtained two clusters with a total of 1717 proteins that were depleted of known contaminants and enriched in EV markers with independently validated 71% true-positive. These clusters had 133 clusters of differentiation (CD) antigens and were enriched with proteins from cell-to-cell communication and signaling. We compared our data with the proteins deposited in PeptideAtlas, making our refined EV protein list a resource for mechanistic and biomarker studies. As a use case example for this resource, we validated the type 1 diabetes biomarker proplatelet basic protein in EVs and showed that it regulates apoptosis of β cells and macrophages, two key players in the disease development. Our approach provides a refinement of the EV composition and a resource for the scientific community.
Disentangling Jenny’s equation by machine learning
Authors: Prieto-Castrillo F. , Rodríguez-Rastrero M. , Yunta F. , Borondo F. , Borondo J. 
Published: November 2023

AbstractThe so-called soil-landscape model is the central paradigm which relates soil types to their forming factors through the visionary Jenny’s equation. This is a formal mathematical expression that would permit to infer which soil should be found in a specific geographical location if the involved relationship was sufficiently known. Unfortunately, Jenny’s is only a conceptual expression, where the intervening variables are of qualitative nature, not being then possible to work it out with standard mathematical tools. In this work, we take a first step to unlock this expression, showing how Machine Learning can be used to predictably relate soil types and environmental factors. Our method outperforms other conventional statistical analyses that can be carried out on the same forming factors defined by measurable environmental variables.
Genomic and transcriptomic characterization of pre-operative chemotherapy response in patients with osteosarcoma
Authors: Yang Yongkun , Huang Zhen , Yuan Mingming , Rui Jinqiu , Chen Rongrong , Jin Tao , Sun Yang , Deng Zhiping , Shan Huachao , Niu Xiaohui , Liu Weifeng 
Published: November 2023

AbstractOsteosarcoma is a heterogeneous disease with regard to its chemotherapy response and clinical outcomes. This study aims to investigate the genomic and transcriptomic characteristics related to pre-operative chemotherapy response. Samples from 25 osteosarcoma patients were collected to perform both whole exome and transcriptome sequencing. Osteosarcoma had significant amount of chromosomal copy number variants (CNVs). Chemotherapy responders showed the higher chromosomal CNV burden than non-responders (p = 0.0775), but the difference was not significant. The percentage of COSMIC signature 3, associated with homologous recombination repair deficiency, was higher in responders (56%) than in non-responders (45%). Transcriptomic analysis suggested that 11 genes were significantly up-regulated in responders and 18 genes were up-regulated in non-responders. Both GSEA and KEGG enrichment analysis indicted that four pathways related to cardiomyopathy were up-regulated in responders, while neuroactive ligand − receptor interaction was up-regulated in non-responders. Finally, a previously published chemoresistant model was validated using our dataset, with the area under the curve of 0.796 (95% CI, 0.583–1.000). Osteosarcoma had the heterogeneous mutational profile with frequent occurrence of CNVs. Transcriptomic analysis identified several signaling pathways associated with chemotherapy responsiveness to osteosarcoma. Transcriptomic signatures provides a potential research direction for predicting the chemotherapy response.
Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress
Authors: Yang Yaru , Li Shuaidong , Zhu Yumin , Che Litao , Wu Qifan , Bai Shijun , Shu Guocheng , Zhao Xianming , Guo Peng , Soaud Salma A. , Li Nianzhen , Deng Mengling , Li Jia , El-Sappah Ahmed H. 
Published: November 2023

AbstractBecause China produces the most crayfish in the world, safe solutions must be improved to mitigate the risks of ongoing heavy metal stressors accumulation. This study aimed to use Saccharomyces cerevisiae as a bioremediation agent to counteract the harmful effect of cadmium (Cd) on crayfish (Procambarus clarkia). Our study used three concentrations of S. cerevisiae on crayfish feed to assess their Cd toxicity remediation effect by measuring total antioxidant capacity (TAC) and the biomarkers related to oxidative stress like malondialdehyde (MDA), protein carbonyl derivates (PCO), and DNA–protein crosslink (DPC). A graphite furnace atomic absorption spectroscopy device was used to determine Cd contents in crayfish. Furthermore, the mRNA expression levels of lysozyme (LSZ), metallothionein (MT), and prophenoloxidase (proPO) were evaluated before and following the addition of S. cerevisiae. The results indicated that S. cerevisae at 5% supplemented in fundamental feed exhibited the best removal effect, and Cd removal rates at days 4th, 8th, 12th, and 21st were 12, 19, 29.7, and 66.45%, respectively, which were significantly higher than the basal diet of crayfish. The addition of S. cerevisiae increased TAC levels. On the other hand, it decreased MDA, PCO, and DPC, which had risen due to Cd exposure. Furthermore, it increased the expression of proPO, which was reduced by Cd exposure, and decreased the expression of LSZ and MT, acting in the opposite direction of Cd exposure alone. These findings demonstrated that feeding S. cerevisiae effectively reduces the Cd from crayfish and could be used to develop Cd-free crayfish-based foods.
Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes
Authors: Seki Shinsuke , Kawabe Toshiaki , Yamazaki Wataru , Matsumura Kazuaki , Oikawa Takanori , Obata Takahiro , Higashiya Misako , Yano Megumi , Eto Tomoo 
Published: November 2023

AbstractIntracellular ice formation during cryopreservation is lethal to the cell, including during warming. Here, we examined the effect of sample volume and warming rate on the cryopreservation success of 1-cell rat embryos based on successful development into blastocysts in vitro and to term in vivo following embryo transfer. Embryos were equilibrated in 5% propylene glycol solution for 10 min, held for 40 s at 0 °C in cryopreservation solution (5%PG + PEPeS), and cooled by immersion in liquid nitrogen. When 1-cell embryos were cryopreserved in a volume of 30–100 μL at a cooling rate of 5830–7160 °C/min and warmed at 35,480–49,400 °C/min by adding 1 mL of 0.3 M sucrose solution at 50 °C, 17.3–28.8% developed into blastocysts, compared with 57.0% of untreated embryos. However, when 1-cell embryos were cryopreserved in a smaller volume of 15 μl at 7950 °C/min and warmed at 68,850 °C/min, 58.8 ± 10.6% developed into blastocysts and 50.0 ± 7.4% developed to term, comparable to that of non-treated embryos (57.0 ± 5.4% and 51.4 ± 3.1%, respectively). Cryopreserved embryos at other developmental stages also showed high in vitro culture potential similar to that of the control. Using a conventional cryotube and a small-volume vitrification procedure with rapid warming, we achieved high levels of subsequent rat embryonic development at all developmental stages.