bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is Chronic Fatigue Syndrome a Connective Tissue Disorder? A Cross-Sectional Study in Adolescents
Authors: van de Putte E.M. , Uiterwaal C.S.P.M. , Bots M.L. , Kuis W. , Kimpen J.L.L. , Engelbert R.H.H. 
Published: April 2005

Objectives. To investigate whether constitutional laxity of the connective tissues is more frequently present in adolescents with chronic fatigue syndrome (CFS) than in healthy controls. Increased joint hypermobility in patients with CFS has been previously described, as has lower blood pressure in fatigued individuals, which raises the question of whether constitutional laxity is a possible biological predisposing factor for CFS.Design. Cross-sectional study.Participants. Thirty-two adolescents with CFS (according to the criteria of the Centers for Disease Control and Prevention) referred to a tertiary hospital and 167 healthy controls.Methods. The 32 adolescents with CFS were examined extensively regarding collagen-related parameters: joint mobility, blood pressure, arterial stiffness and arterial wall thickness, skin extensibility, and degradation products of collagen metabolism. Possible confounding factors (age, gender, height, weight, physical activity, muscle strength, diet, alcohol consumption, and cigarette smoking) were also measured. The results were compared with findings in 167 healthy adolescents who underwent the same examinations.Results. Joint mobility, Beighton score, and collagen biochemistry, all indicators of connective tissue abnormality, were equal for both groups. Systolic blood pressure, however, was remarkably lower in patients with CFS (117.3 vs. 129.7 mm Hg; adjusted difference: −13.5 mm Hg; 95% confidence interval [CI]: −19.1, −7.0). Skin extensibility was higher in adolescents with CFS (mean z score: 0.5 vs. 0.1 SD; adjusted difference: 0.3 SD; 95% CI: 0.1, 0.5). Arterial stiffness, expressed as common carotid distension, was lower in adolescents with CFS, indicating stiffer arteries (670 vs 820 μm; adjusted difference: −110 μm; 95% CI: −220, −10). All analyses were adjusted for age, gender, body mass index, and physical activity. Additionally, arterial stiffness was adjusted for lumen diameter and pulse pressure.Conclusions. These findings do not consistently point in the same direction of an abnormality in connective tissue. Patients with CFS did have lower blood pressure and more extensible skin but lacked the most important parameter indicating constitutional laxity, ie, joint hypermobility. Moreover, the collagen metabolism measured by crosslinks and hydroxyproline in urine, mainly reflecting bone resorption, was not different. The unexpected finding of stiffer arteries in patients with CFS warrants additional investigation.
The Utility of Safety Counseling in a Pediatric Emergency Department
Authors: Claudius Ilene A. , Nager Alan L. 
Published: April 2005

Objective. Pediatric injuries have a significant impact on the medical system, costing lives and disabling many survivors. Although injury-prevention measures do exist, they are underutilized. A majority of families do not consistently receive counseling by a primary care provider (PCP). We attempted to demonstrate the efficacy of counseling families who presented to a pediatric emergency department with unrelated medical complaints.Methods. A self-report questionnaire was administered to assess the home safety of patients 2 weeks to 12 years old presenting to the emergency department. Targeted counseling on areas noted to be unsafe was provided, and a 2-week follow-up telephone call was made to assess the effectiveness of the counseling. Information on previous counseling by a PCP was also collected and analyzed. Logistic regression was performed to determine significance and calculate odds ratios.Results. Thirty-seven percent of caregivers recalled receiving any counseling at a PCP visit. Caregivers who had received prior counseling by a PCP were significantly more likely to have a safe home environment. Patients who were English speaking were significantly more likely to have received safety counseling than their Spanish-speaking counterparts. One hundred fifty families received counseling in the emergency department, and 117 were eligible for follow-up. Of these families, 39% made a positive change in the safety of their child's environment at the 2-week telephone follow-up.Conclusions. Caregivers receiving counseling by a PCP are more likely to provide a safe home environment for their children. Spanish-speaking patients are at particularly high risk of not receiving counseling. Of caregivers reporting unsafe practices during an unrelated emergency-department encounter, targeted counseling made a positive impact on behavior after discharge.
Correlates of Stress Fractures Among Preadolescent and Adolescent Girls
Authors: Loud Keith J. , Gordon Catherine M. , Micheli Lyle J. , Field Alison E. 
Published: April 2005

Objective. Although stress fractures are a source of significant morbidity in active populations, particularly among young female athletes, the causes of stress fractures have not been explored among females &lt;17 years of age or in the general population. The purpose of this study was to examine correlates of stress fractures in a large, population-based, national, cohort study of preadolescent and adolescent girls.
Methods. A cross-sectional analysis of data from 5461 girls, 11 to 17 years of age, in the Growing Up Today Study, an ongoing longitudinal study of the children of registered female nurses participating in Nurses' Health Study II, was performed. Mothers self-reported information regarding their children's histories of stress fractures on their 1998 annual questionnaire. Growing Up Today Study participants self-reported their weight and height, menarcheal status, physical activity, dietary intake, and disordered eating habits on annual surveys.
Results. In 1998, the mean age of the participants was 13.9 years. Approximately 2.7% of the girls had a history of stress fracture, 3% engaged in disordered eating (using fasting, diet pills, laxatives, or vomiting to control weight), and 16% participated in ≥16 hours per week of moderate to vigorous activity. Age at menarche, z score of BMI in 1998, calcium intake, vitamin D intake, and daily dairy intake were all unrelated to stress fractures after controlling for age. Independent of age and BMI, girls who participated in ≥16 hours per week of activity in 1998 had 1.88 greater odds of a history of stress fracture than did girls who participated in &lt;4 hours per week (95% confidence interval [CI]: 1.18–3.30). Girls who participated in ≥16 hours per week of activity were also more likely than their peers to engage in disordered eating (4.6% vs 2.8%); however, disordered eating did not have an independent association with stress fractures (odds ratio [OR]: 1.33; 95% CI: 0.61–2.89). Independent of age and BMI, each hour per week of high-impact activity significantly increased the risk of stress fracture (OR: 1.05; 95% CI: 1.02–1.09). Among the high-impact physical activities, only running (OR: 1.13; 95% CI: 1.05–1.22) and cheerleading/gymnastics (OR: 1.10; 95% CI: 1.01–1.21) were independently associated with greater odds of stress fracture.
Conclusions. These findings suggest that, although activity can be beneficial for bone health, there is a threshold over which the risk of stress fracture increases significantly among adolescent girls. High-impact activities, particularly running, cheerleading, and gymnastics, appear to be higher risk than other activities. Prospective studies are needed to explore the directionality of these relationships, as well as the role of menstrual history. In the meantime, clinicians should remain vigilant in identifying and treating disordered eating and menstrual irregularities among their highly active, young, female patients.
A Comparison of 2 Influenza Vaccine Schedules in 6- to 23-Month-Old Children
Authors: Englund Janet A. , Walter Emmanuel B. , Fairchok Mary P. , Monto Arnold S. , Neuzil Kathleen M. 
Published: April 2005

Background. Trivalent inactivated influenza vaccine (TIV) is recommended for all children ages 6 to 23 months. Delivering 2 doses of TIV at least 4 weeks apart to young children receiving this vaccine for the first time is challenging.Methods. We compared the immunogenicity and reactogenicity of the standard 2-dose regimen of TIV administered in the fall with an early schedule of a single spring dose followed by a fall dose of the same vaccine in healthy toddlers 6 to 23 months of age. Children were recruited in the spring to be randomized into either the standard or early schedule. An additional group was also enrolled in the fall as part of a nonrandomized standard comparison group. The 2002–2003 licensed TIV was administered in the spring; the fall 2003–2004 vaccine contained the same 3 antigenic components. Reactogenicity was assessed by parental diaries and telephone surveillance. Blood was obtained after the second dose of TIV for all children. The primary outcome measure was antibody response to influenza A/H1N1, A/H3N2, and B after 2 doses of vaccine, as determined by hemagglutination-inhibition titers ≥1:32 and geometric mean titer (GMT).Results. Two hundred nineteen children were randomized to receive either the standard or early TIV schedule; 40 additional children were enrolled in the fall in the nonrandomized standard group. Response rates in the combined standard versus early groups were similar overall: 78% (GMT: 48) vs 76% (GMT: 57) to H1N1, 89% (GMT: 115) vs 88% (GMT: 129) to H3N2, and 52% (GMT: 24) vs 60% (GMT: 28) to B. Reactogenicity after TIV in both groups of children was minimal and did not differ by dose, age, or time between doses. Reaction rates were higher in those receiving TIV and concomitant vaccines compared with those receiving TIV alone. Overall rates of fever &gt;38°C axillary and injection-site pain, redness, or swelling were 5.4%, 3.1%, 0.9%, and 1.1%, respectively.Conclusions. When the spring and fall influenza vaccines had the same 3 antigenic components, the early vaccine schedule resulted in similar immunogenicity and reactogenicity compared with the standard schedule. When the vaccine components do not change between years, initiating influenza vaccine in the spring at the time of routine office visits would facilitate full immunization of children against influenza earlier in the season.
Whole-rock and mineral chemistry of cumulates from the Kızıldağ (Hatay) ophiolite (Turkey): clues for multiple magma generation during crustal accretion in the southern Neotethyan ocean
Authors: Bağci U. , Parlak O. , Höck V. 
Published: March 2005

AbstractThe late Cretaceous Kızıldağ ophiolite forms one of the best exposures of oceanic lithospheric remnants of southern Neotethys to the north of the Arabian promontory in Turkey. The ultramafic to mafic cumulate rocks, displaying variable thickness (ranging from 165 to 700 m), are ductiley deformed, possibly in response to syn-magmatic extension during sea-floor spreading and characterized by wehrlite, olivine gabbro, olivine gabbronorite and gabbro. The gabbroic cumulates have an intrusive contact with the wehrlitic cumulates in some places. The crystallization order of the cumulus and intercumulus phases is olivine (Fo86–77)± chromian spinel, clinopyroxene (Mg#92–76), plagio-clase(An95–83), orthopyroxene(Mg#87–79). The olivine, clinopyroxene, orthopyroxene and plagioclase in ultramafic and mafic cumulate rocks seem to have similar compositional range. This suggests that these rocks cannot represent a simple crystal line of descent. Instead the overlapping ranges in mineral compositions in different rock types suggest multiple magma generation during crustal accretion for the Kızıldağ ophiolite. The presence of high Mg# of olivine, clinopyroxene, orthopyroxene, and the absence of Ca-rich plagioclase as an early fractionating phase co-precipitating with forsteritic olivine, suggest that the Kızıldağ plutonic suite is not likely to have originated in a mid-ocean ridge environment. Instead the whole-rock and mineral chemistry of the cumulates indicates their derivation from an island arc tholeiitic (IAT) magma. All the evidence indicates that the Kızıldağ ophiolite formed along a slow-spreading centre in a fore-arc region of a suprasubduction zone tectonic setting.
Naldrettite, Pd2Sb, a new intermetallic mineral from the Mesamax Northwest deposit, Ungava region, Québec, Canada
Authors: Cabri L. J. , McDonald A. M. , Stanley C. J. , Rudashevsky N. S. , Poirier G. , Durham B. R. , Mungall J. E. , Rudashevsky V. N. 
Published: March 2005

AbstractNaldrettite, Pd2Sb, is a new intermetallic mineral discovered in the Mesamax Northwest deposit, Cape Smith fold belt, Ungava region, northern Québec. It is associated with monoclinic pyrrhotite, pentlandite, chalcopyrite, galena, sphalerite, cobaltite, clinochlore, magnetite, sudburyite (PdSb), electrum and altaite. Other rarer associated minerals include a second new mineral (ungavaite, Pd4Sb3), sperrylite (PtAs2), michenerite (PdBiTe), petzite (Ag3AuTe4) and hessite (Ag2Te). Naldrettite occurs as anhedral grains, which are commonly attached or moulded to sulphide minerals, and also associated with clinochlore. Grains of naldrettite vary in size (equivalent circle diameter) from ~10 to 239 μm, with an average of 74.4 mm (n = 632). Cleavage was not observed and fracture is irregular. The mineral has a mean micro-indentation hardness of 393 kg/mm2. It is distinctly anisotropic, non-pleochroic, has weak bireflectance, and does not exhibit discernible internal reflections. Some grains display evidence of strain-induced polysynthetic twinning. Naldrettite appears bright creamy white in association with pentlandite, pyrrhotite, clinochlore and chalcopyrite. Reflectance values in air (and in oil) for R1 and R2 are: 49.0, 50.9 (35.9, 37.6) at 470 nm, 53.2, 55.1 (40.3, 42.1) at 546 nm, 55.4, 57.5 (42.5, 44.3) at 589 nm and 58.5, 60.1 (45.4, 47.2) at 650 nm. The average of 69 electron-microprobe analyses on 19 particles gives: Pd 63.49, Fe 0.11, Sb 35.75, As 0.31, and S 0.02, total 99.68 wt.%, corresponding to (Pd1.995Fe0.007)2.002(Sb0.982AS0.014S0.002)0.998. The mineral is orthorhombic, space group Cmc21, a 3.3906(1), b 17.5551(5), c 6.957(2) Å , V 414.097(3) Å3, Z = 8. Dcalc is 10.694(1) g/cm3. The six strongest lines in the X-ray powder-diffraction pattern [d in Å (I)(hkl)] are: 2.2454(100)(132), 2.0567(52)(043), 2.0009(40)(152), 1.2842(42)(115), 1.2122(50)(204) and 0.8584(56)(1.17.4).
A simplified method for analysis of polyunsaturated fatty acids
Authors: Kang Jing X , Wang Jingdong 
Published: March 2005

Abstract
Background
Analysis of fatty acid composition of biological materials is a common task in lipid research. Conventionally, preparation of samples for fatty acid analysis by gas chromatography involves two separate procedures: lipid extraction and methylation. This conventional method is complicated, tedious and time consuming. Development of a rapid and simple method for lipid analysis is warranted.

Results
We simplified the conventional method by combining the extraction and methylation into a single step (omitting the procedure of prior extraction). Various biological samples including cultured cells, animal tissues and human specimens have been tested using the new method. Statistical analysis indicates that the recovery of long chain fatty acids from tissue samples by the simplified method is significantly higher than that by the traditional method, but there is no difference in relative fatty acid composition between the two methods. This simplified method can significantly save time and materials, and reduce the potentials of sample loss and contamination.

Conclusion
The lipid extraction procedure prior to methylation employed conventionally in lipid analysis can be omitted without affecting the recovery of long chain (≥ 18 C) fatty acids and their composition. The simplified method is rapid, easy-to-use, suitable for analysis of total long chain polyunsaturated fatty acid contents (e.g. n-6 and n-3 fatty acids) in various biological samples, especially when the number of samples to be analyzed is large and/or the specimen size is small.
Providing visualisation support for the analysis of anatomy ontology data
Authors: Dadzie Aba-Sah , Burger Albert 
Published: March 2005

Abstract
Background
Improvements in technology have been accompanied by the generation of large amounts of complex data. This same technology must be harnessed effectively if the knowledge stored within the data is to be retrieved. Storing data in ontologies aids its management; ontologies serve as controlled vocabularies that promote data exchange and re-use, improving analysis.
The Edinburgh Mouse Atlas Project stores the developmental stages of the mouse embryo in anatomy ontologies. This project is looking at the use of visual data overviews for intuitive analysis of the ontology data.

Results
A prototype has been developed that visualises the ontologies using directed acyclic graphs in two dimensions, with the ability to study detail in regions of interest in isolation or within the context of the overview. This is followed by the development of a technique that layers individual anatomy ontologies in three-dimensional space, so that relationships across multiple data sets may be mapped using physical links drawn along the third axis.

Conclusion
Usability evaluations of the applications confirmed advantages in visual analysis of complex data. This project will look next at data input from multiple sources, and continue to develop the techniques presented to provide intuitive identification of relationships that span multiple ontologies.
An Entropy-based gene selection method for cancer classification using microarray data
Authors: Liu Xiaoxing , Krishnan Arun , Mondry Adrian 
Published: March 2005

Abstract
Background
Accurate diagnosis of cancer subtypes remains a challenging problem. Building classifiers based on gene expression data is a promising approach; yet the selection of non-redundant but relevant genes is difficult.
The selected gene set should be small enough to allow diagnosis even in regular clinical laboratories and ideally identify genes involved in cancer-specific regulatory pathways. Here an entropy-based method is proposed that selects genes related to the different cancer classes while at the same time reducing the redundancy among the genes.

Results
The present study identifies a subset of features by maximizing the relevance and minimizing the redundancy of the selected genes. A merit called normalized mutual information is employed to measure the relevance and the redundancy of the genes. In order to find a more representative subset of features, an iterative procedure is adopted that incorporates an initial clustering followed by data partitioning and the application of the algorithm to each of the partitions. A leave-one-out approach then selects the most commonly selected genes across all the different runs and the gene selection algorithm is applied again to pare down the list of selected genes until a minimal subset is obtained that gives a satisfactory accuracy of classification.
The algorithm was applied to three different data sets and the results obtained were compared to work done by others using the same data sets

Conclusion
This study presents an entropy-based iterative algorithm for selecting genes from microarray data that are able to classify various cancer sub-types with high accuracy. In addition, the feature set obtained is very compact, that is, the redundancy between genes is reduced to a large extent. This implies that classifiers can be built with a smaller subset of genes.
NXT2 is required for embryonic heart development in zebrafish
Authors: Huang Haigen , Zhang Bo , Hartenstein Parvana A , Chen Jau-nian , Lin Shuo 
Published: March 2005

Abstract
Background
NXT2 is a member of NXT family proteins that are generally involved in exporting nuclear RNA in eukaryotic cells. It is not known if NXT2 has any function in specific biological processes.

Results
A zebrafish mutant exhibiting specific heart defects during embryogenesis was generated by animal cloning-mediated retroviral insertions. Molecular analysis indicated that the mutant phenotype was caused by a disruption of NXT2. Whole-mount RNA in situ hybridization showed that NXT2 transcripts were clearly detectable in embryonic heart as well as other tissues. Further analysis revealed that expression level of one form of alternative splicing NXT2 mRNA transcripts was significantly reduced, resulting in deficient myocardial cell differentiation and the malformation of cardiac valve at the atrioventricular boundary. The defects could be reproduced by morpholino anti-sense oligo knockdown of NXT2.

Conclusion
NXT2 has a critical role in maintaining morphogenetic integrity of embryonic heart in vertebrate species.
Genomic organization, annotation, and ligand-receptor inferences of chicken chemokines and chemokine receptor genes based on comparative genomics
Authors: Wang Jixin , Adelson David L , Yilmaz Ahmet , Sze Sing-Hoi , Jin Yuan , Zhu James J 
Published: March 2005

Abstract
Background
Chemokines and their receptors play important roles in host defense, organogenesis, hematopoiesis, and neuronal communication. Forty-two chemokines and 19 cognate receptors have been found in the human genome. Prior to this report, only 11 chicken chemokines and 7 receptors had been reported. The objectives of this study were to systematically identify chicken chemokines and their cognate receptor genes in the chicken genome and to annotate these genes and ligand-receptor binding by a comparative genomics approach.

Results
Twenty-three chemokine and 14 chemokine receptor genes were identified in the chicken genome. All of the chicken chemokines contained a conserved CC, CXC, CX3C, or XC motif, whereas all the chemokine receptors had seven conserved transmembrane helices, four extracellular domains with a conserved cysteine, and a conserved DRYLAIV sequence in the second intracellular domain. The number of coding exons in these genes and the syntenies are highly conserved between human, mouse, and chicken although the amino acid sequence homologies are generally low between mammalian and chicken chemokines. Chicken genes were named with the systematic nomenclature used in humans and mice based on phylogeny, synteny, and sequence homology.

Conclusion
The independent nomenclature of chicken chemokines and chemokine receptors suggests that the chicken may have ligand-receptor pairings similar to mammals. All identified chicken chemokines and their cognate receptors were identified in the chicken genome except CCR9, whose ligand was not identified in this study. The organization of these genes suggests that there were a substantial number of these genes present before divergence between aves and mammals and more gene duplications of CC, CXC, CCR, and CXCR subfamilies in mammals than in aves after the divergence.