bluuebunny/crossref_metadata_2025_split huggingface dataset:
Betaine prevents loss of sialic acid residues and peroxidative injury of erythrocyte membrane in ethanol‐given rats
Authors: Kanbak Güngör , Özdemir Filiz , Çalışkan Figen , Şahin Fezan , İnal Mine 
Published: September 2005

AbstractTo evaluate chronic ethanol toxicity on erythrocyte membrane and preventive action of betaine as a methyl donor, 24 male Wistar albino rats were divided into three groups: control, ethanol and ethanol plus betaine group. Animals were fed 60 ml diet per day for two months. Rats in the ethanol group were fed ethanol 8 g/kg/day. The ethanol + betaine groups were fed ethanol plus betaine (0.5% w/v). After two months, all animals were killed. Malondialdehyde (MDA) and sialic acid (SA) levels were determined in plasma samples. Osmotic fragility tests were performed on whole blood samples and erythrocyte membrane thiol contents were determined using membrane suspensions.Plasma MDA levels in ethanol‐given rats were increased significantly compared to the control group of rats (p < 0.05). MDA in the betaine group was significantly lower than that in the ethanol group (p < 0.05). Erythrocyte membrane thiol contents in ethanol group were decreased compared with those of the control group (p < 0.05). Thiol contents were increased slightly after betaine therapy, but this increase was not statistically significant (p > 0.05). Plasma sialic acid levels in the ethanol group were significantly higher than in the control group (p < 0.05). Sialic acid was decreased in the betaine group compared to the ethanol group (p < 0.05). In the osmotic fragility test, we observed that chronic ethanol consumption increased erythrocyte hemolysis. Betaine protected against ethanol‐induced hemolysis.Our findings show that chronic ethanol administration affects erythrocyte membrane properties and this may be related to oxidative stress. Betaine protects erythrocyte membrane alterations against chronic ethanol toxicity. Therefore betaine as a nutritional agent, may protect ethanol induced clinical problems associated with membrane abnormalities. Copyright © 2005 John Wiley & Sons, Ltd.
A study of glutathione status in the blood and tissues of patients with breast cancer
Authors: Yeh Chih‐Ching , Hou Ming‐Feng , Wu Szu‐Hsien , Tsai Shih‐Meng , Lin Shu‐Kai , Hou Linda Ann , Ma Hsu , Tsai Li‐Yu 
Published: September 2005

AbstractGlutathione plays an important role in the antioxidant system that is required for the maintenance of the redox status of the cell, defence against free radicals and detoxification of toxic compounds. Reduced glutathione (redGSH) can be converted to oxidized glutathione (GSSG) during oxidative stress. The ratio of redGSH/total glutathione can be regarded as an index of the redox status and a useful indicator of disease risks. We conducted experiments by the capillary zone electrophoresis method to investigate the alterations of the glutathione status in the blood and tissue samples from patients with breast cancer. The results showed that the levels of redGSH, GSSG, total glutathione and the ratio of redGSH/total glutathione were significantly decreased in the blood of the patients with breast cancer compared to those of the control subjects. The levels of various forms of glutathione were lower and more pronounced in stage III. In contrast, the levels of redGSH, GSSG, total glutathione and the redGSH/total glutathione ratio in breast cancer tissues were significantly increased relative to those of the adjacent cancer‐free tissues, especially in stage II. We suggest that the high redGSH levels are associated with the enhancement of cell proliferation and resistance to apoptosis in the cancer cells, and the loss of the large amount of erythrocyte redGSH may be due to increased detoxification capacities and defence against oxidative stress. We propose that redGSH should be regarded as an important biochemical parameter for detecting breast malignancy. Copyright © 2005 John Wiley & Sons, Ltd.
Incidence, retrieval methods, and outcomes of stent loss during percutaneous coronary intervention: A large single‐center experience
Authors: Brilakis Emmanouil S. , Best Patricia J.M. , Elesber Ahmad A. , Barsness Gregory W. , Lennon Ryan J. , Holmes David R. , Rihal Charanjit S. , Garratt Kirk N. 
Published: September 2005

AbstractOur goal was to examine the incidence and consequences of stent loss during percutaneous coronary intervention (PCI) and the retrieval techniques used. We retrospectively reviewed 11,773 consecutive PCI cases involving stents performed at our institution between January 1994 and March 2004 to identify cases of stent loss. Stent loss occurred in 38 of 11,773 PCI procedures involving stents (0.32%; 95% CI = 0.23–0.44%). Mean age of the patients was 67 ± 11 years and 82% were men. Stent loss occurred more frequently in lesions with calcification and/or significant proximal angulation. In three patients, the stent was crushed and covered with another stent without attempting retrieval. Stent retrieval was attempted in 35 of 38 cases and was successful in 30 (86%). The following retrieval methods were used (more than one method was used in some cases): advancing a balloon through the stent, inflating the balloon, and withdrawing the stent (45%); twirling two wires around the stent (5%); loop snare (26%); biliary forceps (12%); Cook retained fragment retriever (10%); and basket retrieval device (2%). Patients in whom stent loss occurred had a higher incidence of bleeding requiring transfusion (24% vs. 7%; P < 0.001) and more often required emergency coronary artery bypass surgery (5% vs. 0.4%; P < 0.001). No patients in whom the stent was crushed or deployed in the coronary artery had any major cardiac complication. Stent loss during PCI occurs infrequently. Lost stents can be successfully retrieved in the majority of cases using a variety of retrieval techniques, yet stent loss is associated with an increased risk of complications. Stent deployment or crushing may be a good alternative to retrieval. © 2005 Wiley‐Liss, Inc.
Novel Anti‐Markovnikov Regioselectivity in the Wacker Reaction of Styrenes
Authors: Wright Joseph A. , Gaunt Matthew J. , Spencer Jonathan B. 
Published: September 2005

AbstractThe Wacker reaction is one of the longest known palladium‐catalysed organic transformations, and in the vast majority of cases proceeds with Markovnikov regioselectivity. Palladium(II)‐mediated oxidation of styrenes was examined and in the absence of reoxidants was found to proceed in an anti‐Markovnikov sense, giving aldehydes. Studies on the mechanism of this unusual transformation were carried out, and indicate the possible involvement of a η4‐palladium–styrene complex. With a heteropolyacid as the terminal oxidant, oxidation of styrene to give the anti‐Markovnikov aldehyde as the major product was found to be catalytic.
Mononuclear, Dinuclear, and Pentanuclear [{N,S(thiolate)}Iron(II)] Complexes: Nuclearity Control, Incorporation of Hydroxide Bridging Ligands, and Magnetic Behavior
Authors: Krishnamurthy Divya , Sarjeant Amy N. , Goldberg David P. , Caneschi Andrea , Totti Federico , Zakharov Lev N. , Rheingold Arnold L. 
Published: September 2005

AbstractThe mixed N3S(thiolate) ligand 1‐[bis{2‐(pyridin‐2‐yl)ethyl}amino]‐2‐methylpropane‐2‐thiol (Py2SH) was used in the synthesis of four iron(II) complexes: [(Py2S)FeCl] (1), [(Py2S)FeBr] (2), [(Py2S)4Fe5II(μ‐OH)2](BF4)4 (3), and [(Py2S)2Fe2II(μ‐OH)]BF4 (4). The X‐ray structures of 1 and 2 revealed monomeric iron(II)–alkylthiolate complexes with distorted trigonal‐bipyramidal geometries. The paramagnetic 1H NMR spectra of 1 and 2 display resonances from δ=−25 ppm to +100 ppm, consistent with a high‐spin iron(II) ion (S=2). Spectral assignments were made on the basis of chemical shift information and T1 measurements and show the monomeric structures are intact in solution. To provide entry into hydroxide‐containing complexes, a novel synthetic method was developed involving strict aprotic conditions and limiting amounts of H2O. Reaction of Py2SH with NaH and Fe(BF4)2⋅6 H2O under aprotic conditions led to the isolation of the pentanuclear, μ‐OH complex 3, which has a novel dimer‐of‐dimers type structure connected by a central iron atom. Conductivity data on 3 show this structure is retained in CH2Cl2. Rational modification of the ligand‐to‐metal ratio allows control over the nuclearity of the product, yielding the dinuclear complex 4. The X‐ray structure of 4 reveals an unprecedented face‐sharing, biooctahedral complex with an [S2O] bridging arrangement. The magnetic properties of 3 and 4 in the range 1.9–300 K were successfully modeled. Dinuclear 4 is antiferromagnetically coupled [J=−18.8(2) cm−1]. Pentanuclear 3 exhibits ferrimagnetic behavior, with a high‐spin ground state of ST=6, and was best modeled with three different exchange parameters [J=−15.3(2), J′=−24.7(3), and J′′=−5.36(7) cm−1]. DFT calculations provided good support for the interpretation of the magnetic properties.
Molecular Design of Glycoprotein Mimetics: Glycoblotting by Engineered Proteins with an Oxylamino‐Functionalized Amino Acid Residue
Authors: Matsubara Naoki , Oiwa Kei , Hohsaka Takahiro , Sadamoto Reiko , Niikura Kenichi , Fukuhara Norio , Takimoto Akio , Kondo Hirosato , Nishimura Shin‐Ichiro 
Published: September 2005

AbstractThe general and efficient method for the site‐directed glycosylation of proteins is a key step in order to understand the biological importance of the carbohydrate chains of proteins and to control functional roles of the engineered glycoproteins in terms of the development of improved glycoprotein therapeutics. We have developed a novel method for site‐directed glycosylation of proteins based on chemoselective blotting of common reducing sugars by genetically encoded proteins. The oxylamino‐functionalized L‐homoserine residues, 2‐amino‐4‐O‐(N‐methylaminooxy) butanoic acid and 2‐amino‐4‐aminooxy butanoic acid, were efficiently incorporated into proteins by using the four‐base codon/anticodon pair strategy in Escherichia coli in vitro translation. Direct and chemoselective coupling between unmodified simple sugars and N‐methylaminooxy group displayed on the engineered streptavidin allowed for the combinatorial synthesis of novel glycoprotein mimetics.
From Dysfunction to Bis‐function: On the Design and Applications of Functionalised Ionic Liquids
Authors: Fei Zhaofu , Geldbach Tilmann J. , Zhao Dongbin , Dyson Paul J. 
Published: September 2005

AbstractSome of the recent developments concerning the synthesis, properties and applications of functionalised ionic liquids are highlighted. Various strategies are presented, including functionalisation of the cation, anion or both cation and anion in the same ionic liquid, leading to what has been termed dual‐functionalised ionic liquids. Particular attention is given to the application of functionalised ionic liquids as reaction media, to stabilise nanoparticles/modify surfaces and to generate porous materials.
In situ Sol—Gel Composition of Inorganic/Organic Polymeric Hybrid Precursors to Synthesize Red‐Luminescent CaTiO3:Pr3+ and CaTi0.5Zr0.5O3:Pr3+ Phosphors.
Authors: Yan Bing , Zhou Kun 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Evidence for the Existence of β‐Na3AlH6: Monitoring the Phase Transformation from α‐Na3AlH6 by in situ Methods.
Authors: Weidenthaler C. , Pommerin A. , Felderhoff M. , Schmidt W. , Bogdanovic B. , Schueth F. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Photocatalytic Performance of Ba5Ta4O15 to Decomposition of H2O into H2 and O2.
Authors: Otsuka Harumi , Kim Kiyoung , Kouzu Asuka , Takimoto Ikuharu , Fujimori Hirotaka , Sakata Yoshihisa , Imamura Hayao , Matsumoto Takako , Toda Kenji 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Assembly of Helical Hydrogen Bonds in a New Layered Aluminophosphate [C6N3H17] [Al2(HPO4)(PO4)2].
Authors: Wang Mei , Li Ji‐yang , Yu Ji‐hong , Pan Qin‐he , Song Xiao‐wei , Xu Ru‐ren 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Fundamental Studies of (H3Ge)xSiH4‐x Molecules: Precursors to Semiconductor Hetero‐ and Nanostructures on Si.
Authors: Ritter Cole J. , Hu Changwu , Chizmeshya Andrew V. G. , Tolle John , Klewer Douglas , Tsong Ignatius S. T. , Kouvetakis John 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Poly[diaquahexakis(dimethyl sulfoxide‐ϰO)cerium [(μ2‐Hexacosaoxooctamolybdate)sodium]].
Authors: Chen Li‐Juan , Lu Can‐Zhong , Zhang Quan‐Zheng , Chen Shu‐Mei 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Non‐C2‐Symmetric, Chirally Economical, and Readily Tunable Linked‐Binols: Design and Application in a Direct Catalytic Asymmetric Mannich‐Type Reaction.
Authors: Yoshida Takamasa , Morimoto Hiroyuki , Kumagai Naoya , Matsunaga Shigeki , Shibasaki Masakatsu 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Fluoride‐Induced Nucleophilic (Phenylthio)difluoromethylation of Carbonyl Compounds with [Difluoro(phenylthio)methyl]trimethylsilane (TMS—CF2SPh).
Authors: Prakash G. K. Surya , Hu Jinbo , Wang Ying , Olah George A. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Convenient Synthesis of Difluoromethyl Alcohols from Both Enolizable and Non‐Enolizable Carbonyl Compounds with Difluoromethyl Phenyl Sulfone.
Authors: Prakash G. K. Surya , Hu Jinbo , Wang Ying , Olah George A. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Tow‐Carbon Homologation of Ketones via Sily Ketene Acetals: Synthesis of α,β‐Unsaturated Acids and α‐Trimethylsilyl δ‐Ketoacids.
Authors: Bellassoued Moncef , Mouelhi Sinda , Fromentin Pierre , Gonzalez Aurelien 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Silicasulfuric Acid/NaNO2 as a New Reagent for Deprotection of S,S‐Acetals under Solvent‐Free Conditions.
Authors: Hajipour Abdol R. , Zarei Amin , Khazdooz Leila , Pourmousavi Seied Ali , Ruoho Arnold E. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Bis(4,5‐dimethoxy‐2‐nitrophenyl)ethylene Glycol: A New and Efficient Photolabile Protecting Group for Aldehydes and Ketones.
Authors: Kantevari Srinivas , Narasimhaji Ch. Venkata , Mereyala Hari Babu 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
The Rhodium Catalyzed Three‐Component Reaction of Diazoacetates, Titanium(IV) Alkoxides and Aldehydes.
Authors: Lu Chong‐Dao , Liu Hui , Chen Zhi‐Yong , Hu Wen‐Hao , Mi Ai‐Qiao 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Novel Lipophilic and/or Fluorophilic Ethers of Perfluoro‐tert‐butyl Alcohol, Perfluoropinacol and Hexafluoroacetone Hydrate via a Mitsunobu Reaction: Typical Cases of Ideal Product Separation.
Authors: Szabo Denes , Bonto Ana‐Maria , Koevesdi Istvan , Goemoery Agnes , Rabai Jozsef 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Perfluorinated Carboxylic Acid Membrane Monomers by Utilizing Liquid‐Phase Direct Fluorination.
Authors: Okazoe Takashi , Watanabe Kunio , Itoh Masahiro , Shirakawa Daisuke , Kawahara Kengo , Tatematsu Shin 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
β‐Cyclodextrin Promoted Allylation of Aldehydes with Allyltributyltin under Supramolecular Catalysis in Water.
Authors: Krishnaveni N. Srilakshmi , Surendra K. , Kumar V. Pavan , Srinivas B. , Reddy C. Suresh , Rao K. Rama 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Medium‐Dependent Lithiated Side Products in the Reductive Lithiation of Allylic Phenyl Thioethers. Diethyl Ether versus Tetrahydrofuran.
Authors: Screttas Constantinos G. , Heropoulos Georgios A. , Micha‐Screttas Maria , Steele Barry R. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Pd2(dba)3/Xantphos‐Catalyzed Cross‐Coupling of Thiols and Aryl Bromides/Triflates
Authors: Mispelaere‐Canivet Clotilde , Spindler Jean‐Francis , Perrio Stephane , Beslin Pierre 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Optically Pure 1,2‐Diaryl‐ and 1,2‐Alkylaryl‐1,2‐amino Sulfides.
Authors: Arroyo Yolanda , Meana Angela , Rodriguez J. Felix , Santos Mercedes , Sanz‐Tejedor M. Ascension , Garcia Ruano Jose L. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Facile Synthesis of Multisubstituted Buta‐1,3‐dienes via Suzuki—Miyaura and Kumada Cross‐Coupling Strategy of 2,4‐Diiodo‐buta‐1‐enes with Arylboronic Acids and Grignard Reagents.
Authors: Shao Li‐Xiong , Shi Min 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Benzothieno[3,2‐b]indole Derivatives as Potent Selective Estrogen Receptor Modulators.
Authors: Ji Qinggang , Gao Jie , Wang Junbo , Yang Chunhao , Hui Xin , Yan Xueming , Wu Xihan , Xie Yuyuan , Wang Ming‐Wei 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Regioselective Synthesis of 1,5‐Diaryl‐1H‐imidazoles by Palladium‐Catalyzed Direct Arylation of 1‐Aryl‐1H‐imidazoles.
Authors: Bellina Fabio , Cauteruccio Silvia , Mannina Luisa , Rossi Renzo , Viel Stephane 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Direct Catalytic Synthesis of Enantiopure 5‐Substituted Oxazolidinones from Racemic Terminal Epoxides.
Authors: Bartoli Giuseppe , Bosco Marcella , Carlone Armando , Locatelli Manuela , Melchiorre Paolo , Sambri Letizia 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
1,1‐Dichloropolyfluoroalkanesulfenamides and Their Dehydrochlorination Effected by Triethylamine.
Authors: Timoshenko V. M. , Rudnichenko A. V. , Tkachenko A. V. , Shermolovich Yu. G. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Herbicidal Activity of 2‐Alkyl(aryl)‐3‐methylsulfonyl(sulfinyl)pyrano [4,3‐c]pyrazol‐4(2H)‐ones.
Authors: Li Yu Xin , Wang You Ming , Liu Bin , Wang Su Hua , Li Zheng Ming 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis, Reaction and Antimicrobial Activity of 2‐Amino‐4‐(8‐quinolinol‐5‐yl)‐1‐(p‐tolyl)‐pyrrole‐3‐carbonitrile.
Authors: Abdel‐Mohsen Shawkat A. 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Phosphodiesterase 5 Inhibitory Activity of Novel Pyrido[1,2‐e]purin‐4(3H)‐one Derivatives.
Authors: Xia Guangxin , Li Jianfeng , Peng Aiming , Lai Shunan , Zhang Shujun , Shen Jingshan , Liu Zhonghua , Chen Xinjian , Ji Ruyun 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Single Step Synthesis of 2,3‐Dialkyl‐6‐nitro‐quinazolin‐4(3H)‐imines and 3,5‐Dialkyl‐9‐nitro‐imidazo[1,2‐c]quinazolin‐2(3H)‐ones.
Authors: Erba Emanuela , Pocar Donato , Trimarco Pasqualina 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Intramolecular Cyclization of δ‐Iminoacetylenes: A New Entry to Pyrazino[1,2‐a]indoles.
Authors: Abbiati Giorgio , Arcadi Antonio , Bellinazzi Alessandra , Beccalli Egle , Rossi Elisabetta , Zanzola Simona 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Regio‐ and Stereoselective Approach to 1,2‐Di‐ and 1,1,2‐Trisilylethenes by Cobalt‐Mediated Reaction of Silyl‐Substituted Dibromomethanes with Silylmethylmagnesium Reagents.
Authors: Ohmiya Hirohisa , Yorimitsu Hideki , Oshima Koichiro 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Steroids. Part 418. Synthesis, Reactions, Conformation Analysis, and NMR Spectra of 5,10‐Epoxy‐5ξ,10ξ‐estrane‐3,17‐diones.
Authors: Budesinsky Milos , Fajkos Jan , Guenter Jaroslav , Kasal Alexander 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Asymmetric Synthesis of Both Enantiomers of syn‐(3‐Trifluoromethyl)cysteine Derivatives.
Authors: Jiang Zhong‐Xing , Liu Xiao‐Ping , Qiu Xiao‐Long , Qing Feng‐Ling 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Isolation, Characterization and Antiplasmodial Activity of Steroidal Alkaloids from Funtumia elastica (Preuss) Stapf.
Authors: Zirihi Guede Noel , Grellier Philipp , Guede‐Guina Frederick , Bodo Bernard , Mambu Lengo 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of All‐trans Arachidonic Acid and Its Effect on Rabbit Platelet Aggregation.
Authors: Anagnostopoulos Dimitris , Chatgilialoglu Chryssostomos , Ferreri Carla , Samadi Abdelouahid , Siafaka‐Kapadai Athanassia 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Lambertellol C, a Labile and Novel Biosynthetic Congener of Lambertellols A and B.
Authors: Murakami Takanori , Sasaki Akane , Fukushi Eri , Kawabata Jun , Hashimoto Masaru , Okuno Toshikatsu 
Published: September 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Comparison of two methods for enumerating circulating tumor cells in carcinoma patients
Authors: Balic Marija , Dandachi Nadia , Hofmann Günter , Samonigg Hellmut , Loibner Hans , Obwaller Andreas , van der Kooi Alexander , Tibbe Arjan G. J. , Doyle Gerald V. , Terstappen Leon W. M. M. , Bauernhofer Thomas 
Published: September 2005

AbstractBackgroundMonitoring of circulating tumor cells (CTCs) in blood of carcinoma patients treated with novel compounds may be a measurement of treatment effectiveness. Before it can be used clinically, a reliably method is needed to enumerate CTCs. We compared two methods for CTC enumeration, OnkoQuick and the CellSearch system.MethodsWe drew 22.5 ml of blood into three CellSave tubes from 15 healthy donors and 61 patients with metastatic carcinoma. After pooling, 15 ml was processed with OncoQuick and 7.5 ml with CellSearch.ResultsWith both methods no CTCs were found in healthy donors. At least one CTC was detected in 14 of 61 patients (23%) with OncoQuick and 33 of 61 patients (54%) with CellSearch (P < 0.0001). The number of CTCs detected was larger for CellSearch (mean 20 CTCs/7.5 ml of blood) than for OncoQuick (3 CTCs/7.5 ml; P < 0.0001).ConclusionThe CellSearch system is a more accurate and sensitive method to enumerate CTCs. Further studies are warranted to evaluate CTC enumeration by the CellSearch system as a monitoring tool for the evaluation of the efficacy of novel anticancer agents. © 2005 Wiley‐Liss, Inc.