bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychiatric Disorders and Dietary Habits: An Investigation using Mendelian Randomization Analysis and Insights into Gene Expression Regulatory Mechanisms
Authors: Feng Yu , Shen Jing 
Published: October 2023

Abstract
Background
Dietary habits play a crucial role in life and may influence the development of psychiatric disorders (PD). Although observational studies have suggested an association between dietary habits and PD, establishing causality remains challenging due to confounding factors and study limitations.
Methods
In this study, we utilized dietary data from a vast cohort of over 500,000 participants recruited by the UK Biobank, along with genome-wide association study (GWAS) data for 11 PD. Leveraging two-sample Mendelian randomization (TSMR) and summary data-based Mendelian randomization (SMR) analyses, we integrated expression, methylation, and protein quantitative trait loci (eQTL, mQTL, and pQTL) data from brain tissue and blood.
Results
Our TSMR analyses revealed significant correlations between the intake of Cheese, Alcohol, and Dried fruit and multiple PD. SMR analyses identified several genes associated with these dietary habits, some of which were also linked to an increased risk of various PD. Furthermore, we observed a consistent association between the dietary habit-associated protein C1-Esterase Inhibitor and the risk of Insomnia, Major Depressive Disorder (MDD), and Neuroticism.
Conclusions
Our findings suggest a potential link between dietary habits and PD and shed light on plausible mechanisms underlying gene expression regulation. These results hold essential implications for future research on the pathogenesis of PD and the intricate relationship between diet and mental health. However, further investigations are warranted to validate and gain a deeper understanding of these associations, exploring additional contributing factors and elucidating the underlying mechanisms.
Effects of anticipatory humanitarian cash assistance to households forecasted to experience extreme flooding: evidence from Bangladesh
Authors: Gros Clemens , Pronti Andrea , Sheikh Khairul , Hassan Ahmadul , Shahjahan Mohammad 
Published: October 2023

Abstract

The 2020 monsoon floods in Bangladesh were among the most severe and protracted in decades. Instead of waiting for disaster to strike, the Bangladesh Red Crescent Society used impact-based forecast data to reach nearly 3,800 vulnerable households along the Jamuna River with a one-off unconditional cash transfer of BDT 4,500 (about $53) before peak flooding in July 2020. Anticipatory action to help at-risk populations avoid or mitigate extreme weather event impacts has become widely used by governments and humanitarian organisations worldwide. However, robust evaluations of the effectiveness of forecast-based assistance are limited. This assessment follows a quasi-experimental approach, drawing on survey data from a sample of cash recipients and equally vulnerable and flood-affected households that were not reached by BDRCS before the flood. Our analysis finds robust statistical evidence that the intervention was effective in helping households evacuate the flood-affected area, protecting personal health and well-being, and safeguarding people's productive assets and livestock. It was also effective in enabling beneficiaries to avoid taking on high-interest loans and selling valuable assets during and after the flood. The intervention does not appear to have helped cash recipients avoid food-based coping mechanisms or regain their productive capacity sooner after the flood.
Beyond the Dusty Fog: Local Eye Drop Therapy and Potentially New
Treatment Alternatives in Pseudoexfoliative Glaucoma
Authors: Zeppieri Marco , Musa Mutali 
Published: October 2023

Abstract:
Pseudoexfoliative glaucoma (PEG) is a type of secondary open-angle glaucoma
characterized by the accumulation of whitish-gray material on the trabecular meshwork
and lens, leading to an increase in intraocular pressure (IOP) and optic nerve damage. Local
eye drop therapy is one of the first-line treatments for PEG, which include prostaglandin
analogues, beta-blockers, and alpha-adrenergic agonists to lower IOP. New treatments
beyond conventional techniques, however, are constantly being developed. One potential
treatment proposed for PEG is based on magnetic phage display, which involves using
magnetic nanoparticles conjugated to specific peptides or proteins selected using phage
display techniques to remove aggregates in the anterior chamber of the eye or inflammatory
cells and cytokines that contribute to PEG pathogenesis. Other potential treatments include
microRNAs (miRNAs) that are involved in the regulation of gene expression at the
post-transcription stages. Gene therapies, nanotechnology, immunotherapy and methods
based on stem cells can also be potentially used to target and treat specific tissues and
cells responsible for regulating IOP. In addition, photobiomodulation therapy (PBMT), a
non-invasive procedure that utilizes low-level laser therapy to improve cellular function
and promote tissue repair, can prove an interesting alternative in treating PEG. The aim of
our mini-review is to provide a brief overview of these innovative methods that appear to
offer potentially promising treatment options for PEG.
The Potential Role of Cardiokines in Heart and Kidney Diseases
Authors: Yang Ming , Luo Shilu , Yang Jinfei , Chen Wei , He Liyu , Liu Di , Wang Xi , Sun Lin 
Published: October 2023

Abstract:
As the engine that maintains blood circulation, the heart is also an endocrine
organ that regulates the function of distant target organs by secreting a series of cardiokines.
As endocrine factors, cardiokines play an indispensable role in maintaining the
homeostasis of the heart and other organs. Here, we summarize some of the cardiokines
that have been defined thus far and explore their roles in heart and kidney diseases. Finally,
we propose that cardiokines may be a potential therapeutic target for kidney diseases.
Remarkable Effects of a Rhenium(I)-diselenoether Drug on the Production of
Cathepsins B and S by Macrophages and their Polarizations
Authors: Collery Philippe , Desmaële Didier , Harikrishnan Adhikesavan , Veena Vijay 
Published: October 2023

Background/Objective:
Tumor-associated macrophages (TAMs) produce an excessive amount of
cysteine proteases, and we aimed to study the effects of anticancer rhenium(I)-diselenoether (Re-diSe) on the
production of cathepsins B and S by macrophages. We investigated the effect of Re-diSe on lipopolysaccharides
(LPS) induced M1 macrophages, or by interleukin 6 (IL-6) induced M2 macrophages.

Methods:
Non-stimulated or prestimulated murine Raw 264 or human THP-1 macrophages were exposed to
increasing concentrations of the drug (5, 10, 20, 50 and 100 μM) and viability was assayed by the MTT assay.
The amount of cysteine proteases was evaluated by ELISA tests, the number of M1 and M2 macrophages by
the expression of CD80 or CD206 biomarkers. The binding of Re-diSe with GSH as a model thiol-containing
protein was studied by mass spectrometry.

Results:
A dose-dependent decrease in cathepsins B and S was observed in M1 macrophages. There was no
effect in non-stimulated cells. The drug induced a dramatic dose-dependent increase in M1 expression in both
cells, significantly decreased the M2 expression in Raw 264 and had no effect in non-stimulated macrophages.
The binding of the Re atom with the thiols was clearly demonstrated.

Conclusion:
The increase in the number of M1 and a decrease in M2 macrophages treated by Re-diSe could
be related to the decrease in cysteine proteases upon binding of their thiol residues with the Re atom.
Ras-related Protein in Brain 4A (Rab4A) is Downregulated by miR-496 to inhibit the Progression of Gastric Cancer
Authors: Su Rui , Su Meng , Lu Yan 
Published: October 2023

Introduction:
Ras-related protein in brain 4A (Rab4A), as a member of the Rab family,
is involved in the intracellular circulation of membrane receptors or endocytic substances and
regulates the progression of multiple tumors.

Methods:
From our results, the knockdown of Rab4A inhibited the proliferation, migration and invasion
in AGS cells. Importantly, the surface expression of epidermal growth factor receptor (EGFR)
declined significantly in Rab4A knockdown cells. The downstream pathway of EGFR was also inhibited
after the transfection of Rab4A-specific siRNA, including AKT and β-catenin pathways.

Result:
In addition, miR-496 down-regulated the expression of Rab4A in AGS cells. The result
of the luciferase reporter assay showed that miR-496 could bind to the 3’UTR of Rab4A.

Conclusion:
In conclusion, the expression of Rab4A is inhibited by miR-496, and the knockdown
of Rab4A inhibits the proliferation, migration and invasion through down-regulating the
surface expression of EGFR. Rab4A is a potential target in the treatment of gastric cancer.
Phytochemistry and Pharmacological Activity of Malva sylvestris L:
A Detailed Insight
Authors: Paul Zahid Ahmad , Malla Aamir Tariq , Dar Mohammad Akbar , Masoodi Mubashir Hussain 
Published: October 2023

:
Malva sylvestris L., is commonly referred to as Mallow and is found in Europe, Asia
and Africa. This has been traditionally used for inflammation, gastrointestinal disturbances, skin
disorders, menstrual pains, and urological disorders. This review covers phytoconstituents and
Pharmacological activities of M. sylvestris. The plant contains a large number of phytochemical
constituents having diverse pharmacological activities. The plant contains many phenolic compounds
responsible for its strong antioxidant activity. Coumarins from Mallow have a potential
anticancer activity. Malva sylvestris also contains essential as well as non-essential elements and
minerals. Many researchers have provided evidence that Malva sylvestris is a good candidate for
use as a medicinal herb and has good nutritional value. The leaves, in particular, offer properties
like anticancer, skin whitening, and anti-aging. Furthermore, the aqueous extract was recently
shown to have an anti-ulcerogenic effect. Malva sylvestris has a high potential for use in cosmetics
such as skin whitening and anti-aging treatments. Methanolic extracts of Malva sylvestris
leaves, and flowers showed strong antibacterial activity against a common plant pathogen bacterium.
The plant also contains Malvone A, which is responsible for antibacterial action. The plant
also possesses anti-inflammatory, analgesic, wound healing properties and various other activities.
Network-Pharmacology-Based Study on Active Phytochemicals and Molecular Mechanism of Cnidii Fructus in Treating Colorectal Cancer
Authors: Wei Zhihui , Zhang Xiaoyun , Peng Antang , Liu Chenxu , Pang Jianying , Zhang Yajing , Duan Xuhong 
Published: October 2023

Aims:
Cnidii Fructus (CF) is known for its antibacterial, anti-inflammatory, and antitumor
properties, as well as its activities against kidney deficiency and impotence. In this study,
we aimed to explore the anti-CRC cancer effect and molecular mechanism of CF via network
pharmacology and in vitro antitumor experiments.

Methods:
Network pharmacology was used to investigate the anti-CRC mechanism of CF. First,
a series of databases was used to screen the active phytochemical targets and anti-CRC core targets.
Then, the GO and KEGG pathways were analyzed to predict possible mechanisms. Molecular
docking analysis explore core targets-phytochemicals interactions. In vitro antitumor experiments
were carried on verifying anti-CRC mechanism of CF.

Results:
In this study, 20 active ingredient targets and 50 intersecting targets were analyzed by
Cytoscape software 3.9.1 to obtain the core genes and phytochemicals. Then, the GO and KEGG
pathways of 50 intersecting targets were analyzed to predict possible mechanisms. The results
from GO and KEGG indicated that CF has significant antitumor efficacy, which involves many
signaling pathways, such as PI3K/AKT and p53. The five core targets and five core phytochemicals
were screened for molecular docking to show protein-ligand interactions. According to the
results of molecular docking, the compound O-acetylcolumbianetin was selected for the anti-
CRC functional verification in vitro. MTT assay showed that O-acetylcolumbianetin significantly
inhibited the proliferation of colorectal HCT116 cells in a time- and quantity-dependent manner.
O-acetylcolumbianetin can promote the expression of CASP3 protein, induce HCT116 cells
apoptosis, thus exert anti-CRC effect.

Conclusion:
This study preliminarily verified the anti-CRC effect and molecular mechanism of
CF and provided a reference for Traditional Chinese Medicine anti-tumor subsequent research.
BAO-Ag-NPs as Promising Suppressor of ET-1/ICAM-1/VCAM-1
Signaling Pathway in ISO-induced AMI in Rats
Authors: Mosaad Yasser O. , Ateyya Hayam , Hussein Mohammed A. , Moro Ahmed M. , Abdel-Wahab Ebtsam A. , El-Ella Amr A. , Nassar Zahraa N. 
Published: October 2023

Objectives:
Acute myocardial infarction (AMI) is the most prevalent cause of myocardial
fibrosis and the leading cause of mortality from cardiovascular disease. The goal of this
work was to synthesize Balanites aegyptiaca oil-silver nanoparticles (BAO-Ag-NPs) and evaluate
their cardioprotective effect against ISO-induced myocardial infarction in rats, as well as
their mechanism.

Materials and Methods:
BAO was isolated, and the unsaturated fatty acids were estimated.
BAO-Ag-NPs was prepared, LD50 was calculated to evaluate its cardioprotective activity
against ISO (85 mg/kg)-induced AMI. Different doses of BAO-Ag-NPs (1/50 LD50; 46.6
mg/kg.b.w and 1/20 LD50; 116.5 mg) were received to the rats.

Results:
The total fatty acids and unsaturated fatty acids generated by BAO were 909.63 and
653.47 mg/100 g oil, respectively. Oleic acid methyl ester, 9-octadecenoic acid methyl ester, and
9, 12-Octadecadienoic acid methyl ester were the predominant ingredients, with concentrations
of 107.6, 243.42, and 256.77 mg/100 g oil, respectively. According to TEM and DLS examinations,
BAO-Ag-NPs have a size of 38.20 ± 2.5 nm and a negative zeta potential of -19.82 ± 0.30
mV, respectively. The LD50 of synthesized BAO-Ag-NPs is 2330 mg. On the other hand, BAOAg-
NPs reduce myocardial necrosis by lowering increased BNP, cTnI, CK-MB, TC, TG, MDA,
MMP2, TGF-β1, PGE2, and IL-6 levels. Furthermore, BAO-Ag-NPs inhibit the expression of
ET-1, ICAM-1, and VCAM-1 genes as well as enhance HDL-C, CAT, and GSH levels when
compared to the ISO-treated group of rats. Histopathological findings suggested that BAO-Ag-
NPs enhance cardiac function by increasing posterior wall thickness in heart tissues.

Conclusion:
BAO-Ag-NPs protect against AMI in vivo by regulating inflammation, excessive
autophagy, and oxidative stress, as well as lowering apoptosis via suppression of the ET-1,
ICAM-1, and VCAM-1 signaling pathways.
Research Progress of Eucommia ulmoides Oliv and Predictive Analysis of
Quality Markers Based on Network Pharmacology
Authors: Zhao Xiaomei , Qu Qiong , Zhang Ying , Zhao Peiyuan , Qiu Jinqing , Zhang Xinbo , Duan Xi , Song Xiao 
Published: October 2023

Abstract:
Du Zhong is a valuable Chinese medicinal herb unique to China. It is a national second-
class precious protected tree, known as “plant gold”, which has been used to treat various
diseases since ancient times. The main active ingredients are lignans, phenylprophetons, flavonoids,
iridoids and steroids and terpenoids, which have pharmacological effects such as lowering
blood pressure, enhancing immunity, regulating bone metabolism, protecting nerve cells, protecting
liver and gallbladder and regulating blood lipids. In this paper, a comprehensive review
of Eucommia ulmoides Oliv. was summarized from the processing and its compositional changes,
applications, chemical components, pharmacological effects, and pharmacokinetics, and the
Q-marker of Eucommia ulmoides Oliv. is preliminarily predicted from the aspects of traditional
efficacy, medicinal properties and measurability of chemical composition, and the pharmacodynamic
substance basis and potential Q-marker of Eucommia ulmoides Oliv. are further analyzed
through network pharmacology. It is speculated that quercetin, kaempferol, β-sitosterol, chlorogenic
acid and pinoresinol diglucoside components are selected as quality markers of Eucommia
ulmoides Oliv., which provide a basis for the quality control evaluation and follow-up research
and development of Eucommia ulmoides Oliv.
Viaminate Inhibits Propionibacterium Acnes-induced Abnormal Proliferation
and Keratinization of HaCat Cells by Regulating the S100A8/S100A9-
MAPK Cascade
Authors: Cao Junjie , Xu Meifeng , Zhu Longfei , Xiao Shengxiang 
Published: October 2023

Background:
Viaminate, a vitamin A acid drug developed in China, has been clinically
used in acne treatment to regulate epithelial cell differentiation and proliferation, inhibit keratinization,
reduce sebum secretion, and control immunological and anti-inflammatory actions; however,
the exact method by which it works is unknown.

Methods:
In the present study, acne was induced in the ears of rats using Propionibacterium acnes
combined with sebum application

Results:
After 30 days of treatment with viaminate, the symptoms of epidermal thickening and keratin
overproduction in the ears of rats were significantly improved. Transcriptomic analysis of rat
skin tissues suggested that viaminate significantly regulated the biological pathways of cellular
keratinization. Gene differential analysis revealed that the S100A8 and S100A9 genes were significantly
downregulated after viaminate treatment. The results of qPCR and Western blotting confirmed
that viaminate inhibited the expression of S100A8 and S100A9 genes and proteins in rat
and HaCat cell acne models, while its downstream pathway MAPK (MAPK p38/JNK/ERK1/2)
protein expression levels were suppressed. Additional administration of the S100A8 and S100A9
complex protein significantly reversed the inhibitory effect of viaminate on abnormal proliferation
and keratinization levels in acne cell models.

Conclusion:
In summary, viaminate can improve acne by modulating S100A8 and S100A9 to inhibit
MAPK pathway activation and inhibit keratinocyte proliferation and keratinization levels.
Vegetable Mountain Products, as Functional Food. A Cross-sectional
Study of European Romanian Agricultural Producers
Authors: Covaci Brindusa , Brejea Radu Petru , Covaci Mihai 
Published: October 2023

Background::
Mountain areas, in a normal ecosystemic context as the analyzed region
from the European Romanian Carpathians, offer healthier solutions through different agronomical
practices and solutions designed for the production of functional food. The paper approaches a
chain formed by mountain functional food – agronomic practices – mountain products commerce,
the purpose being the development of the entire mountain products value chain from an area focusing on the matrix "from the farm to the fork". The paper analyzes highly consumed mountain products with functional food roles Allium cepa, Allium sativum, Cucumis sativus, Capsicum, and
Solanum lycopersicum.

Objective:
The research highlights the importance of vegetable mountain products as a functional
food in the current hunger and environment contexts, in a more and more polluted world

Methods::
The experimental, clinical, and agronomical research, together with the production territorial profile, show that mountain products present high qualitative valences comparatively with
low-land areas or with reference values given by USDA.

Results::
According to macro-nutritional and micro-nutritional analysis – lipids-fats, saturated fatty acids, protein, cyanocobalamin (B12), ergocalciferol (D2
), cholecalciferol (D3
), iron and calcium
– Allium sativum dominates the mountain product's top with a functional food role. Instead of this,
mountain producers prefer to cultivate mountain products with a higher income horizon.

Conclusion::
Functional food represents an imperative in a more polluted world. Being less polluted than other ecosystems, the mountain area offers healthier agricultural products and requires
notable investments, together with more involved agronomy.
Applications of PET and SPECT in Patients with Autism Spectrum Disorder
Authors: Martini Anna Lisa , Quartuccio Natale , Schiera Irene Giovanna , Berti Valentina , Burroni Luca , Cistaro Angelina 
Published: October 2023

Abstract:
Autism spectrum disorder (ASD) consists of neurological development disorders that manifest before three years of age and affect social
interactions, markedly restricting range of interests and activities, often associated with some degree of intellectual disability. Single-photon
emission computed tomography (SPECT) and positron emission tomography (PET) are non-invasive imaging tools to investigate the function of
the brain in vivo. SPECT and PET studies exploring rCBF and brain glucose metabolism in patients with ASD have been performed, providing
important insights into the brain regions involved in ASD. Abnormalities in serotonergic, dopaminergic, GABAergic, cholinergic, and
glutamatergic systems have been suggested to contribute to the observed distorted brain circuitry associated with ASD. However, the specificity of
such abnormalities needs to be fully clarified because schizophrenia and other psychiatric diseases have been shown to present with comparable
changes in neurotransmitter systems. Neuroinflammation could also play a role in the development of autism. Therefore, ASD is a complicated
process involving a number of factors. It is mandatory to perform more research studies to determine the molecular cornerstone of ASD and to
improve our comprehension of the clinical correlates of ASD.
An Awareness of Pharmacovigilance Among Healthcare Professionals Due
to an Underreporting of Adverse Drug Reactions Issue: A Systematic
Review of the Current State, Obstacles, and Strategy
Authors: Putri Risani Andalasia , Ikawati Zullies , Rahmawati Fita , Yasin Nanang Munif 
Published: October 2023

Background:
Healthcare professionals play an essential role in reporting adverse
drug reactions as part of pharmacovigilance activities. However, adverse drug reactions reported
by healthcare professionals remain low.

Objective:
The aim of this systematic review was to investigate healthcare professionals'
knowledge, awareness, attitude, and practice on pharmacovigilance and adverse drug reaction
reporting, explore the causes of the underreporting issue, and provide improvement strategies.

Methods:
This systematic review was conducted using four electronic databases for original papers,
including PubMed, Scopus, Google Scholar, and Scholar ID. Recent publications from 1st January
2012 to 31st December 2022 were selected. The following terms were used in the search: "awareness",
"knowledge", "adverse drug reaction", "pharmacovigilance", "healthcare professional", and "underreporting
factor". Articles were chosen, extracted, and reviewed by the two authors.

Results:
Twenty-five studies were selected for systematic review. This review found that
24.8%–73.33% of healthcare professionals were unaware of the National Pharmacovigilance
Center. Around 20%–95.7% of healthcare professionals have a positive attitude toward
pharmacovigilance and adverse drug reaction reporting, while 12%–60.8% of healthcare
professionals have experience reporting any adverse drug reaction in their practice. The most
frequently highlighted barriers to pharmacovigilance were a lack of awareness and knowledge
regarding what, when, and to whom to report.

Conclusion:
Underreporting issues require immediate attention among healthcare professionals
due to a lack of awareness and knowledge of pharmacovigilance and adverse drug reaction
reporting. Educational and training program interventions have been suggested by most studies
to address these issues.