bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gut microbiota reflect adaptation of cave-dwelling tadpoles to resource scarcity
Authors: Zhu Wei , Chang Liming , Shi Shengchao , Lu Ningning , Du Simeng , Li Jiatang , Jiang Jianping , Wang Bin 
Published: January 2024

Abstract
Gut microbiota are significant to the host’s nutrition and provide a flexible way for the host to adapt to extreme environments. However, whether gut microbiota help the host to colonize caves, a resource-limited environment, remains unknown. The nonobligate cave frog Oreolalax rhodostigmatus completes its metamorphosis within caves for 3–5 years before foraging outside. Their tadpoles are occasionally removed from the caves by floods and utilize outside resources, providing a contrast to the cave-dwelling population. For both cave and outside tadpoles, the development-related reduction in their growth rate and gut length during prometamorphosis coincided with a shift in their gut microbiota, which was characterized by decreased Lactobacillus and Cellulosilyticum and Proteocatella in the cave and outside individuals, respectively. The proportion of these three genera was significantly higher in the gut microbiota of cave-dwelling individuals compared with those outside. The cave-dwellers’ gut microbiota harbored more abundant fibrolytic, glycolytic, and fermentative enzymes and yielded more short-chain fatty acids, potentially benefitting the host’s nutrition. Experimentally depriving the animals of food resulted in gut atrophy for the individuals collected outside the cave, but not for those from inside the cave. Imitating food scarcity reproduced some major microbial features (e.g. abundant Proteocatella and fermentative genes) of the field-collected cave individuals, indicating an association between the cave-associated gut microbiota and resource scarcity. Overall, the gut microbiota may reflect the adaptation of O. rhodostigmatus tadpoles to resource-limited environments. This extends our understanding of the role of gut microbiota in the adaptation of animals to extreme environments.
Nitrate-driven anaerobic oxidation of ethane and butane by bacteria
Authors: Wu Mengxiong , Li Jie , Lai Chun-Yu , Leu Andy O , Sun Shengjie , Gu Rui , Erler Dirk V , Liu Lian , Li Lin , Tyson Gene W , Yuan Zhiguo , McIlroy Simon J , Guo Jianhua 
Published: January 2024

Abstract
The short-chain gaseous alkanes (ethane, propane, and butane; SCGAs) are important components of natural gas, yet their fate in environmental systems is poorly understood. Microbially mediated anaerobic oxidation of SCGAs coupled to nitrate reduction has been demonstrated for propane, but is yet to be shown for ethane or butane—despite being energetically feasible. Here we report two independent bacterial enrichments performing anaerobic ethane and butane oxidation, respectively, coupled to nitrate reduction to dinitrogen gas and ammonium. Isotopic 13C- and 15N-labelling experiments, mass and electron balance tests, and metabolite and meta-omics analyses collectively reveal that the recently described propane-oxidizing “Candidatus Alkanivorans nitratireducens” was also responsible for nitrate-dependent anaerobic oxidation of the SCGAs in both these enrichments. The complete genome of this species encodes alkylsuccinate synthase genes for the activation of ethane/butane via fumarate addition. Further substrate range tests confirm that “Ca. A. nitratireducens” is metabolically versatile, being able to degrade ethane, propane, and butane under anoxic conditions. Moreover, our study proves nitrate as an additional electron sink for ethane and butane in anaerobic environments, and for the first time demonstrates the use of the fumarate addition pathway in anaerobic ethane oxidation. These findings contribute to our understanding of microbial metabolism of SCGAs in anaerobic environments.
Microbial metabolism influences microplastic perturbation of dissolved organic matter in agricultural soils
Authors: Qiu Xinran , Ma Sirui , Pan Jianrui , Cui Qian , Zheng Wei , Ding Ling , Liang Xujun , Xu Baile , Guo Xuetao , Rillig Matthias C 
Published: January 2024

Abstract
An estimated 258 million tons of plastic enter the soil annually. Joining persistent types of microplastic (MP), there will be an increasing demand for biodegradable plastics. There are still many unknowns about plastic pollution by either type, and one large gap is the fate and composition of dissolved organic matter (DOM) released from MPs as well as how they interact with soil microbiomes in agricultural systems. In this study, polyethylene MPs, photoaged to different degrees, and virgin polylactic acid MPs were added to agricultural soil at different levels and incubated for 100 days to address this knowledge gap. We find that, upon MP addition, labile components of low aromaticity were degraded and transformed, resulting in increased aromaticity and oxidation degree, reduced molecular diversity, and changed nitrogen and sulfur contents of soil DOM. Terephthalate, acetate, oxalate, and L-lactate in DOM released by polylactic acid MPs and 4-nitrophenol, propanoate, and nitrate in DOM released by polyethylene MPs were the major molecules available to the soil microbiomes. The bacteria involved in the metabolism of DOM released by MPs are mainly concentrated in Proteobacteria, Actinobacteriota, and Bacteroidota, and fungi are mainly in Ascomycota and Basidiomycota. Our study provides an in-depth understanding of the microbial transformation of DOM released by MPs and its effects of DOM evolution in agricultural soils.
Temporal colonization and metabolic regulation of the gut microbiome in neonatal oxen at single nucleotide resolution
Authors: Dong Quanbin , Hua Dongxu , Wang Xiuchao , Jiao Yuwen , Liu Lu , Deng Qiufeng , Wu Tingting , Zou Huayiyang , Zhao Chen , Wang Chengkun , Reng Jiafa , Ding Luoyang , Hu Shixian , Shi Jing , Wang Yifeng , Zhang Haifeng , Sheng Yanhui , Sun Wei , Shen Yizhao , Tang Liming , Kong Xiangqing , Chen Lianmin 
Published: January 2024

AbstractThe colonization of microbes in the gut is key to establishing a healthy host-microbiome symbiosis for newborns. We longitudinally profiled the gut microbiome in a model consisting of 36 neonatal oxen from birth up to 2 months postpartum and carried out microbial transplantation to reshape their gut microbiome. Genomic reconstruction of deeply sequenced fecal samples resulted in a total of 3931 metagenomic-assembled genomes from 472 representative species, of which 184 were identified as new species when compared with existing databases of oxen. Single nucleotide level metagenomic profiling shows a rapid influx of microbes after birth, followed by dynamic shifts during the first few weeks of life. Microbial transplantation was found to reshape the genetic makeup of 33 metagenomic-assembled genomes (FDR &lt; 0.05), mainly from Prevotella and Bacteroides species. We further linked over 20 million microbial single nucleotide variations to 736 plasma metabolites, which enabled us to characterize 24 study-wide significant associations (P &lt; 4.4 × 10−9) that identify the potential microbial genetic regulation of host immune and neuro-related metabolites, including glutathione and L-dopa. Our integration analyses further revealed that microbial genetic variations may influence the health status and growth performance by modulating metabolites via structural regulation of their encoded proteins. For instance, we found that the albumin levels and total antioxidant capacity were correlated with L-dopa, which was determined by single nucleotide variations via structural regulations of metabolic enzymes. The current results indicate that temporal colonization and transplantation-driven strain replacement are crucial for newborn gut development, offering insights for enhancing newborn health and growth.
Filamentous prophage Pf4 promotes genetic exchange in Pseudomonas aeruginosa
Authors: Pei Tong-Tong , Luo Han , Wang Yuanyuan , Li Hao , Wang Xing-Yu , Zhang Yi-Qiu , An Ying , Wu Li-Li , Ma Junhua , Liang Xiaoye , Yan Aixin , Yang Liang , Chen Changbin , Dong Tao 
Published: January 2024

Abstract
Filamentous prophages are widespread among bacteria and play crucial functions in virulence, antibiotic resistance, and biofilm structures. The filamentous Pf4 particles, extruded by an important pathogen Pseudomonas aeruginosa, can protect producing cells from adverse conditions. Contrary to the conventional belief that the Pf4-encoding cells resist reinfection, we herein report that the Pf4 prophage is reciprocally and commonly exchanged within P. aeruginosa colonies, which can repair defective Pf4 within the community. By labeling the Pf4 locus with antibiotic resistance and fluorescence markers, we demonstrate that the Pf4 locus is frequently exchanged within colony biofilms, in artificial sputum media, and in infected mouse lungs. We further show that Pf4 trafficking is a rapid process and capable of rescuing Pf4-defective mutants. The Pf4 phage is highly adaptable and can package additional DNA doubling its genome size. We also report that two clinical P. aeruginosa isolates are susceptible to the Pf4-mediated exchange, and the Pf5 prophage can be exchanged between cells as well. These findings suggest that the genetic exchanging interactions by filamentous prophages may facilitate defect rescue and the sharing of prophage-dependent benefits and costs within the P. aeruginosa community.
Tissue-associated and vertically transmitted bacterial symbiont in the coral Pocillopora acuta
Authors: Maire Justin , Tsang Min Ching Sarah Jane , Damjanovic Katarina , Epstein Hannah E , Judd Louise M , Blackall Linda L , van Oppen Madeleine J H 
Published: January 2024

Abstract
Coral microhabitats are colonized by a myriad of microorganisms, including diverse bacteria which are essential for host functioning and survival. However, the location, transmission, and functions of individual bacterial species living inside the coral tissues remain poorly studied. Here, we show that a previously undescribed bacterial symbiont of the coral Pocillopora acuta forms cell-associated microbial aggregates (CAMAs) within the mesenterial filaments. CAMAs were found in both adults and larval offspring, suggesting vertical transmission. In situ laser capture microdissection of CAMAs followed by 16S rRNA gene amplicon sequencing and shotgun metagenomics produced a near complete metagenome-assembled genome. We subsequently cultured the CAMA bacteria from Pocillopora acuta colonies, and sequenced and assembled their genomes. Phylogenetic analyses showed that the CAMA bacteria belong to an undescribed Endozoicomonadaceae genus and species, which we propose to name Candidatus Sororendozoicomonas aggregata gen. nov sp. nov. Metabolic pathway reconstruction from its genome sequence suggests this species can synthesize most amino acids, several B vitamins, and antioxidants, and participate in carbon cycling and prey digestion, which may be beneficial to its coral hosts. This study provides detailed insights into a new member of the widespread Endozoicomonadaceae family, thereby improving our understanding of coral holobiont functioning. Vertically transmitted, tissue-associated bacteria, such as Sororendozoicomonas aggregata may be key candidates for the development of microbiome manipulation approaches with long-term positive effects on the coral host.
Episymbiotic Saccharibacteria induce intracellular lipid droplet production in their host bacteria
Authors: Dong Pu-Ting , Tian Jing , Kobayashi-Kirschvink Koseki J , Cen Lujia , McLean Jeffrey S , Bor Batbileg , Shi Wenyuan , He Xuesong 
Published: January 2024

Abstract
Saccharibacteria (formerly TM7) are a group of widespread and genetically diverse ultrasmall bacteria with highly reduced genomes that belong to Candidate Phyla Radiation, a large monophyletic lineage with poorly understood biology. Nanosynbacter lyticus type strain TM7x is the first Saccharibacteria member isolated from the human oral microbiome. With restrained metabolic capacities, TM7x lives on the surface of, and forms an obligate episymbiotic relationship with its bacterial host, Schaalia odontolytica strain XH001. The symbiosis allows TM7x to propagate but presents a burden to host bacteria by inducing stress response. Here, we employed super-resolution fluorescence imaging to investigate the physical association between TM7x and XH001. We showed that the binding with TM7x led to a substantial alteration in the membrane fluidity of XH001. We also revealed the formation of intracellular lipid droplets in XH001 when forming episymbiosis with TM7x, a feature that has not been reported in oral bacteria. The TM7x-induced lipid droplets accumulation in XH001 was confirmed by label-free Raman spectroscopy, which also unveiled additional phenotypical features when XH001 cells are physically associated with TM7x. Further exploration through culturing XH001 under various stress conditions showed that lipid droplets accumulation was a general response to stress. A survival assay demonstrated that the presence of lipid droplets plays a protective role in XH001, enhancing its survival under adverse conditions. In conclusion, our study sheds new light on the intricate interaction between Saccharibacteria and their host bacteria, highlighting the potential benefit conferred by TM7x to its host and further emphasizing the context-dependent nature of symbiotic relationships.
Courtship and mating behavior of Endoclita signifer (Hepialidae: Lepidoptera) and the male sex pheromones in hairbrushes
Authors: Chen Xiao , Su Xiaoyan , Qiu Zhisong , Xu Yuan , Yang Zhende , Hu Ping 
Published: January 2024

Abstract
Endoclita signifer Walker is the most destructive wood-boring pest of Eucalyptus in China, causing significant economic and ecological damage. As an insect of the primitive Lepidoptera family Hepialidae, E. signifer fly and mat for only 10−20 min at dusk. The courtship and mating behavior of E. signifer adults and whether male moths release sex pheromones are still unknown, especially since transitory flight survival strategies in primitive moths differ from advanced moths like noctuids. In this study, we first observed the courtship and mating behavior of E. signifer by considering the effects of space and then analyzed extracts of male hairbrushes using gas chromatography–electroantennogram detection. Our results indicated that during the courtship period, flying males form courtship fields, lekking, and chase flying females before mating with them; E. signifer were more successful in mating in larger spaces (Length × Width × Height = 9.6 × 7 × 4 m); 5 compounds in the hairbrushes of the male moths which elicited antennal responses of 2 sexes, despite at high concentrations. Combined with it, indicating that communication between male and female may rely on male sex pheromones. These findings can serve as a basis for studying the mechanisms of sex communication in E. signifer and developing sex pheromone-based trapping techniques.
Avadhūta: Examining the Emergence and Institutionalisation of an Antinomian Ascetic
Authors: Kanamarlapudi Sravani 
Published: January 2024

Abstract
In numerous historical and contemporary cases, and in various textual and lived traditions, several Hindu ascetics have been called avadhūtas. Yet, there is little scholarly research on this ascetic designation. Based on an ‘iterative’ textual search of early and medieval Hindu texts, this article examines the literary emergence and early reception of the avadhūta ascetic. I argue that the avadhūta label emerged in the late first-millennium CE and was institutionalised within the āśrama system in the early second-millennium CE. I further posit that the avadhūta’s emergence is probably connected to the systematisation of the jīvanmukti or liberation-while-living doctrine, and that the institutionalisation of the antinomian avadhūta ascetic as a legitimate saṃnyāsin or renouncer correlates with two interrelated factors: medieval advaitins defending the avadhūta from anti-jīvanmukti critics and the increasing theological importance of the āśrama system.
Performing/Informing Rights: Mixing Inclusive Dance and Human Rights Education for Disabled People in Sri Lanka and Nepal
Authors: Waldorf Lars , Marambio Helena-Ulrike , Blades Hetty 
Published: January 2024

AbstractOver several years, the German/Sri Lankan NGO VisAbility has been pioneering a mix of inclusive dance with rights education and advocacy to empower persons with conflict-related, physical impairments, first in Sri Lanka and, more recently, in Nepal. This article captures both the benefits and challenges of using dance for more transformative human rights education in informal settings.
Genetic diversity and population structure of the Asian citrus psyllid in China
Authors: Huang Aijun , Ma Jiayu , Yang Jin , Chen Bo , Zhou Jun , Yi Long 
Published: January 2024

Abstract
The Asian citrus psyllid (ACP) is the main vector of Citrus Huanglongbing, the most damaging citrus disease, causing significant financial losses in the citrus industry. Global warming has expanded the habitat of this pest, allowing it to continue its northward migration to China. Population genetic information of ACP is fundamentally essential for species management. This study investigated the genetic diversity and population structure of Chinese ACP using the mitochondrial cytochrome oxidase subunit I gene by dataset comprised 721 sequences from 27 geographic sites in China. Low haplotype diversity (0.323 ± 0.022) and low nucleotide diversity (0.00071 ± 0.00007) were observed in the entire population, which may indicate recent founder events. Twenty-three haplotypes were identified and clustered into 2 haplogroups: haplogroup I and haplogroup II. Haplogroup II included only 2 unique haplotypes, which occurred exclusively in the Southwest China ACP population. Genetic differentiation analyses were also indicative of Southwest China population was significantly differentiated from the remaining populations. Demographic history analysis showed that ACP population in China has experienced demographic expansion. Our results provided a better understanding of the genetic distribution patterns and structures of ACP populations in China.
Small Area Poverty Estimation Under Heteroskedasticity
Authors: Das Sumonkanti , Chambers Ray 
Published: January 2024

Abstract
Multilevel models with nested errors are widely used in poverty estimation. An important application in this context is estimating the distribution of poverty as defined by the distribution of income within a set of domains that cover the population of interest. Since unit-level values of income are usually heteroskedastic, the standard homoskedasticity assumptions implicit in popular multilevel models may not be appropriate and can lead to bias, particularly when used to estimate domain-specific income distributions. This article addresses this problem when the income values in the population of interest can be characterized by a two-level mixed linear model with independent and identically distributed domain effects and with independent but not identically distributed individual effects. Estimation of poverty indicators that are functionals of domain-level income distributions is also addressed, and a nonparametric bootstrap procedure is used to estimate mean squared errors and confidence intervals. The proposed methodology is compared with the well-known World Bank poverty mapping methodology for this situation, using model-based simulation experiments as well as an empirical study based on Bangladesh poverty data.
Measles, mumps, rubella and varicella antibodies among international and domestic university students
Authors: Dyda Amalie , Broome Audrey , Rawlinson William , Mahimbo Abela , Saha Amit , Kefalas Bill , Seale Holly , Macintyre C Raina , Zwar Nicholas , Gidding Heather F , Heywood Anita E 
Published: January 2024

Abstract

Background
Vaccine-preventable infections are generally well controlled in Australia. However, gaps in immunity can lead to outbreaks and are important to identify. Young adults are a highly mobile population and a potential source of imported infections. We aimed to evaluate anti- measles, mumps, rubella and varicella (MMR&V) IgG seroprevalence and explore factors relating to antibody seropositivity.

Methods
A cross-sectional online survey was conducted among students from a large Australian university to collect demographic, vaccination, infection and travel characteristics. Blood samples were collected to measure MMR&V seroprevalence. Logistic regression was used to identify factors associated with seropositivity.

Results
Among 804 university students, seroprevalence (positive or equivocal) for measles was 82.3% (95% CI 79.6–84.8%), mumps 79.5% (95% CI 76.7–82.3%), rubella 91.5% (95% CI 89.6–93.5%) and varicella 86.2% (95% CI 84.1–88.8%), with 452 (56.2%, 95% CI 52.8–59.6) seropositive to all four viruses. Varicella seropositivity was highest in the older birth cohort (born 1988–1991). Measles seropositivity was higher for international students compared to domestic students. Among international students, mumps seroprevalence was significantly lower than measles and rubella seroprevalence. International travel in the previous 12 months was reported by 63.1% of students, but only 18.2% of travellers reported seeking pre-travel health advice prior to most recent international travel.

Conclusions
Overall, this study suggests immunity to MMR&V is sub-optimal. We found the university student population to be highly mobile and unlikely to seek pre-travel advice; thus, they are a potential source of infection importation. The implementation of university immunization policies could address the gaps identified and our findings can inform the development of targeted vaccination campaigns.
Physical Fitness Tests in the Army—Legitimate Diversity or Randomness?
Authors: Tomczak Andrzej 
Published: January 2024

ABSTRACT
In this article, I present my views on the diversified approaches to assess the physical fitness of soldiers within selected armies worldwide. I discussed the related common features and paradoxes associated with these approaches. For the comparative analysis, national diversity was taken into account and subjective selection of the physical fitness tests conducted in the armies of the United States, Finland, Germany, India, China, Israel, Netherlands, Sweden, Switzerland, United Kingdom, and Poland was done. An analysis of the physical fitness tests reveals significant variations, despite the purpose of the assessment being the same, that is, to evaluate a soldier’s physical preparedness for combat tasks. Based on the analysis, I categorize the variation in physical fitness tests of soldiers at four levels: (1) single-system function tests (e.g., push-ups, sit-ups) vs. multi-system tests (obstacle courses), (2) considering health aspects vs. not considering health aspects during physical fitness tests, (3) motor components vs. coordination components (e.g.,balance), and (4) tests differentiated by gender and age vs.tests not differentiated by gender and age. In conclusion, I am in favor of multi-system tests, comprised of obstacle courses mirroring potential situations in combat tasks. In my opinion, multi-system tests are superior compared to single-system function tests since they enable a comprehensive assessment of effort, coordination, and mental predispositions necessary for functioning in real-life conditions. I also support physical fitness evaluation that is not differentiated based on a soldier’s gender and age, as there is no logical justification for such distinctions.