bluuebunny/crossref_metadata_2025_split huggingface dataset:
Obstetric and neonatal outcomes of extremely obese pregnant women after late preterm gestation
Authors: Choodinatha Harshitha Kallubhavi , Lee Jin , Lee Min Jung , Choi Bo Young , Kim Hyeon Ji , Park Jee Yoon 
Published: October 2023

Abstract

Background
Maternal obesity characterized by high body mass index(BMI) is linked to adverse maternal and neonatal outcomes. However, insufficient data exist about the impact of extreme obesity on pregnancy within the Asian population.
Objective
To determine the obstetric and neonatal outcomes of pregnant women with extreme obesity at birth after late preterm gestation.
Methods
A retrospective study on extremely obese pregnant women with BMI ≥ 40kg/m2, obesity class III, excluding fetal death in utero and preterm births before 34 weeks of gestation. Obstetric and neonatal outcomes were reviewed.
Results
The study involved 94 extremely obese pregnant women (median BMI at delivery: 42.4 kg/m2). In relation to the prepregnancy BMI obesity class II category, higher chronic hypertension rates were found in allegedly extremely obese women compared to those with lower pre-pregnancy BMI (34% vs. 10%, p = 0.012), while the tocolytics use for preterm labour was higher in the BMI < 35.0 kg/m2 group (26% vs. 5%, p = 0.007). Adverse neonatal outcomes, including neonatal intensive care unit admission, use of respiratory support and jaundice were higher in the pre-pregnancy BMI < 35.0 kg/m2 group than the BMI ≥ 35.0 kg/m2 group (all p-values < 0.05). The use of neonatal support increased with lower pre-pregnancy BMI and with higher weight gain during pregnancy.
Conclusions
In extremely obese women(class III) who delivered during late preterm gestation, the use of tocolytics for preterm labour and the incidence of adverse neonatal respiratory outcomes appeared to be higher among those who were less obese before pregnancy than among those who were already extremely obese.
Quality of Life in Women with Breast Cancer Undergoing Neoadjuvant Chemotherapy: Comparison Between PICC and PICC-port
Authors: Pinelli Fulvio , Barbani Francesco , Defilippo Barbara , Fundarò Angela , Nella Alessandra , Selmi Valentina , Romagnoli Stefano , Villa Gianluca 
Published: October 2023

Abstract
Background
Peripherally inserted central catheters (PICCs) and new type of arm-port, the PICC-port, are currently used for neoadjuvant chemotherapy treatment in patients with breast cancer. We aimed to compare Quality of Life (QoL) of patients receiving one of these two devices investigating overall satisfaction, psychological impact as well as the impact on professional, social and sport activities, and local discomfort.
Methods
We did a prospective observational before-after study of PICCs versus PICC-ports. Adult (aged ≥18 years) female with breast cancer candidate to neoadjuvant chemotherapy were included. The primary outcome was QoL according to the Quality-of-Life Assessment Venous Device Catheters (QLAVD) questionnaire assessed 12 months after device implantation.
Results
Between May 2019 and November 2020, of 278 individuals screened for eligibility, 210 were enrolled. PICC-ports were preferred over PICCs with a QLAVD score of 29 [25; 32] vs 31 [26; 36.5] (p = 0.014). Specifically, most QLAVD constructs related to psychological impact, social aspects, and discomfort were in favor of PICC-ports vs PICC, especially in women under the age of 60. Overall, pain scores at insertion and during therapy administration were not significantly different between the two groups, as well as infection, secondary malpositioning, thrombosis or obstruction of the device.
Conclusions
In women with breast cancer undergoing neoadjuvant chemotherapy, PICC-ports were overall better accepted than PICCs in terms of QoL, especially in those who were younger. Device-related complications were similar.
Oncoproteins E6/E7 of the Human Papillomavirus Types 16 &amp; 18 Cooperate in Modulating Oncogenes and Tumor Suppressor Proteins in Colorectal Cancer
Authors: Fernandes Queenie , Therachiyil Lubna , Younis Shahd M , Dermime Said , Moustafa Ala-Eddin Al 
Published: October 2023

Abstract

Background This study presents a novel analysis of the oncogenes and tumor suppressor proteins directly modulated by E6/E7 of high-risk HPV types 16 and 18, in two mutational cell models of colorectal cancer (CRC).Methods Two distinct mutational cell models of colorectal cancer, HCT 116 (KRAS mutant) & HT-29 (TP53 mutant) were transfected with E6/E7 of HPV16 and HPV18, individually and in combination (co-transfection), via lipofection. Further, we utilized a liquid chromatography mass spectrometry (LC-MS/MS) approach to analyze and compare the proteomes of KRAS mutant HCT 116 and TP53 mutant HT-29 cell models of CRC, expressing E6/E7 of HPVs type 16 and 18, both, individually and in combination.Results We generated six stably transfected cell lines, namely, HCT 116 HPV16+, HCT 116 HPV18+, HCT 116 HPV16+/18+, HT-29 HPV16+, HT-29 HPV18+, HT-29 HPV16+/18+. Our proteomics data revealed a significantly higher, HPV-induced, modulation of oncogenes and tumor suppressor proteins in the TP53 mutant model of CRC, as compared to the KRAS mutant cell model (p ≤ 0.01). Less than 1% of the genes were found to be commonly modulated by HPV, between the two models of CRC. In addition, we report that HT-29 cells, expressing E6/E7 of both HPVs 16 and 18, significantly reduced the downregulation of oncogenes as compared to cells expressing E6/E7 of either HPV16 or HPV18 individually (p-value is ≤ 0.00001).Conclusion Our data imply that coinfections with the two most frequently observed high-risk HPV types worldwide, leads to the sustenance of a pro-oncogenic environment in CRC; that is suggestive of the cooperative role of the viruses in inflicting cancer. Further, HPV modulates different sets of oncogenes/tumor suppressor proteins in CRC models of varying mutational backgrounds, thus highlighting the importance of personalized therapies for diseases with mutational heterogeneities.
Identification of autophagy related genes predicting response of trastuzumab resistance in breast cancer patients
Authors: liu yaping , hao xinlei , li yuntao , hong yang 
Published: October 2023

AbstractBackground The emergence of trastuzumab resistance is the barrier to effective clinical outcomes for HER2 + Breast Cancer (BC). However, the relationship between the expression of autophagy related genes in HER2 + BC and their correlation with prognosis remains unclear. This study aims to identify the potential predictive markers through bioinformatics analysis and experiment validation. Methods Gene expression profile dataset GSE29431 was obtained from GEO database. Autophagy related differentially expressed genes (ARGs) of HER2 + BC were identified by R software. Multiple bioinformatics analyses were conducted to identify functional hub genes. The prognostic significance of these hub genes was validated, and the correlations between ARGs, clinicopathological parameters, and patient prognosis were analyzed using The Cancer Genome Atlas (TCGA) cohort. Results A total of 73 ARGs were identified between 41 HER2 + BC patients and 12 normal samples. Functional analysis, including Gene Ontology (GO), protein-protein interaction (PPI), and Kyoto Encyclopedia of Genes and Genomes (KEGG), revealed important functional genes related to macroautophagy, late endosome formation, and ubiquitin-like metabolism, which were identified as autophagy-related hub genes. Additionally, PPP1R15A, VAMP7, PTK6, CASP3 demonstrated strong predictive power in the TCGA cohort through prognostic correlation analysis (p < 0.05). The results of qRT-PCR and immunohistochemistry demonstrated increased expression of VAMP7 and PTK6 in BC patients with trastuzumab resistance, further confirming their prognostic significance in a trastuzumab adjuvant treatment cohort using our clinical data. Conclusions Patients with high expression of VAMP7 and PTK6 exhibit poor efficacy and prognosis in HER2 + BC, particularly in the context of trastuzumab neoadjuvant chemotherapy.
Substituting maize with blends of Solanum tuberosum chips and Ensete ventricosum corm on egg production and quality in Bovans Brown
Authors: Kecheroo Yisehakk Kebede , Weji Gossaye 
Published: October 2023

Abstract
The experiment was conducted in the Sodo district of the Gurage zone, in the central highlands of Ethiopia, to ascertain the effects of substituting enset (Ensete ventricosum) corm and potato (Solanum tuberosum) chips for corn on the quantity and quality of eggs laid by Bovans brown layers. A total of 132 Bovans brown layers aged 24 weeks were used in the investigation. All 132 of the Bovans brown layers in the study were 24 weeks old. In place of maize grain, the layers were fed feed that contained 0% (T1), 15% (T2), 30% (T3), and 45% (T4) of a potato chip and enset corm mixture. The trial was totally randomized and comprised four treatments. With 11 birds apiece, each treatment was carried out three times. A 12-week experiment was conducted. Hens were weighed at the start and end of the trial. Dry matter intake, hen-day egg production (HDEP), egg weight, and egg mass were all tracked daily. Every 15 days, four eggs per replicate were evaluated for four different egg quality metrics (egg shell weight and thickness, albumen weight and height, Haugh unit and yolk weight, and color). Mix of potato chips and enset corms with 3568 kcal/kg DM, 4.28% CP, 0.17% ether extract, and 2.40% crude fiber. The average daily increases of T1 (2.08g), T4 (1.99g), and T3 (1.85g) were comparable and significantly larger (P 0.05) than T3 (1.28g). The egg mass and feed conversion ratios among treatments were statistically comparable (P < 0.05). T1's (81.08%) HDEP was equivalent to and significantly (P 0.05) higher than T3 (84.07%), T4 (83.61%), and T2 (83.34%). Compared to T1 and T3, T4 showed a higher eggshell weight (P 0.05). As a result, enset corm and potato chips can be combined to substitute maize in layer rations when it is scarce in enset and potato-growing regions.
Effects of Sucrose and 6-Benzylaminopurine Concentrations on Shoot Regeneration and Vitrification in Aronia melanocarpa: Insights for Plant Tissue Culture Systems
Authors: Bayhan Nida , Yucesan Buhara 
Published: October 2023

Abstract
This study focuses on investigating the effects of sucrose and 6-Benzylaminopurine (BAP) concentrations on shoot regeneration and the development of vitrification in Aronia melanocarpa. Initially, the presence of BAP, in combination with varying sucrose concentrations, leads to a substantial increase in shoot number. The highest shoot formation is observed in the MS medium containing 3% sucrose and 5.0 mg/L BAP. Furthermore, sucrose concentration plays a crucial role in shoot growth, with higher concentrations promoting more extensive shoot development. However, when sucrose levels were at 3% combined with higher BAP concentrations (1.0 or 2.5 mg/L), an increased incidence of vitrification was observed over time. Interestingly, lower sucrose concentrations (1% or 2%) combined with 0.5 mg/L or 2.5 mg/L BAP initially delayed vitrification but eventually led to its occurrence. Microscopic analysis of leaf samples with varying levels of vitrification indicates significant differences in stoma characteristics, such as density and size, further confirming the detrimental impact of vitrification on cellular structures and physiological processes. The recovery of vitrified plants was evaluated using different growth media combinations. The absence of BAP in the medium led to higher recovery percentages without necrosis, while the addition of 0.5 mg/L BAP promoted shoot growth but potentially inhibited root development. During acclimatization, vitrified plants exhibited stunted shoot growth, shorter and narrower leaves, reduced root numbers and lengths, and decreased survival rates, particularly under lower humidity conditions. The cultivation period required for the recovery of the crop was determined to be six weeks under controlled greenhouse conditions.
Cerebral filariasis infection with Litomosoides in Molossus barnesi (Chiroptera: Molossidae) in the brazilian eastern amazon, with comments on Molossinema Wimsatti Georgi, Georgi, Jiang and Fronguillo, 1987
Authors: Conga David , de Araújo Camille , de Souza Natália , Corrêa Jean , Santos Josye , Figueiredo Edigleise , Bernal Marcella , Andrade Sara , Silva Neuder da , Pereira Washington , Gardner Scott , Notarnicola Juliana 
Published: October 2023

Abstract
During the health evaluation of 30 specimens of bats captured in periurban forest areas in the city of Belém–Pará, Brazil, a case of cerebral filariasis was observed. In the histopathological examination, adult filariae were found in the meningeal membranes in the brain tissue of Molossus barnesi (Molossidae) and classified morphologically as Litomosoides by the shape of the spicules –left spicule with a handle longer than the blade; right spicule curved, with a sclerotized heel supporting a dorsal notch; the area rugosa constituted by a ventral band of small longitudinal crests; tail rounded in males; long esophagus with a slightly glandular distal portion, and a muscular bent vagina. All the specimens lack a stoma (buccal capsule). We compared our filarioids with the description and of specimens of Molossinema wimsatti. Morphological characters from M. wimsatti resemble the genus Litomosoides. Thus, we believe that M. wimsatti is a synonym of L. molossi Esslinger, 1973, and filarioid specimens from material reported by Lichtenfels et al. (1981) and from de Souto et al. (2021) most probably correspond to Litomosoides. We suggest that the reduction of the buccal capsule could be attributable to the ectopic location. No evidence of tissue responses by the host were observed. This is the first record of Litomosoides infecting brain tissue of Molossus barnesi from Brazil, representing a new host record. More specimens of bats should be examined in order to find filarioids in the brain and verify its taxonomic position using molecular techniques.
Comparison of breathing pattern and diaphragmatic motion in patients with unilateral cervical radiculopathy and asymptomatic group
Authors: Yousefiyan Raziyeh , Yoosefinejad Amin Kordi , Jalli Reza , Rezaei Iman 
Published: October 2023

Abstract

Background: Recently, there has been growing interest in the research and management of respiratory dysfunctions in patients with neck pain. Although patients with cervical radiculopathy (CR) are predominantly managed as neuromusculoskeletal patients, currently, there is evidence that they also have pulmonary dysfunction. Although many studies have investigated respiratory dysfunction in patients with chronic neck pain, this is the first study to examine the respiratory motion of the diaphragm in CR. The purpose of this study was to compare the breathing pattern and diaphragmatic excursion in patients with unilateral CR with those in an asymptomatic group.
Methods: Twenty-five patients with unilateral CR and 25 asymptomatic individuals between 30 and 55 years old participated in this study. Diaphragmatic motion, breathing pattern, active cervical range of motion (ACROM) and kinesiophobia were investigated in both groups by using fluoroscopy, manual assessment of respiratory motion (MARM), cervical range of motion device, and Tampa Scale of Kinesiophobia (TSK) questionnaire. Statistical significance was set at 0.05.
Results: No statistically significant differences were found between the two groups with regard to sex, age and body mass index (BMI). The mean excursion of the hemi diaphragm on the involved side (the side of CR) was significantly lower than that on the uninvolved side in patients with unilateral CR with a large effect size. The excursion of the involved hemi diaphragm in patients was reduced compared to the matched hemi diaphragm in the control group. There was no significant difference between the hemi diaphragm excursion in the control group. The results of the MARM variables showed that the volume of breathing and the percentage rib cage motion in normal and deep breathing were significantly different between the two groups, but there was no significant difference in the balance of breathing between the two groups. Additionally, the CROM was reduced in these patients in comparison to the control group, and it was less on the involved side than on the uninvolved side.
Conclusion: The results of this study revealed a dysfunctional breathing pattern in normal and deep breathing and a unilateral reduction in diaphragmatic excursion on the side of radiculopathy in patients with unilateral CR compared to the control group.
Hyper-methionine Feeding Influence diabetes nephropathy (DN) by regulating the methylation status of kidney in STZ-induced diabetes rats
Authors: Jiang Yongwei , Deng GuoXiong , Liu Chengyin , Tang Han , Zheng Jing , Kong Xiaomu , Zhao Meimei , Liu Yi , Gao Peng , Li Tianbao , Zhao Hailing , Cao Yongtong , Li Ping , Ma Liang 
Published: October 2023

Abstract
This study aimed to investigate the impact of hyper-methionine (HM) feeding on diabetic nephropathy (DN) pathogenesis by examining and analyzing differential methylation profiles. Eight-week-old male Wistar rats (250–300g) were randomly divided into four groups: control group (healthy, n = 8), streptozocin (STZ) induced DN rats (STZ group, n = 8), STZ-induced DN rats fed with HM (STZ + HM group, n = 8), and the Tangshen Formula (TSF) treatment for STZ + HM rats (TSF group, n = 8). Blood glucose and other metabolic indicators were monitored before treatment and at four-week intervals until 12 weeks. Total DNA was extracted from the aforementioned groups, and DNA methylation profiles were analyzed using reduced representative bisulfite sequencing (RRBS).The STZ group and STZ + HM group exhibited increased levels of blood glucose and urinary albumin/creatinine compared to control group. STZ + HM group showed significantly higher levels of urinary albumin/creatinine (411.90 ± 88.86 mg/g) than the STZ group (238.41 ± 62.52 mg/g). A total of 71,957 differentially methylated regions (DMRs) were identified, with 48,950 (68.02%) being hyper-methylated and 23,007 (31.98%) being hypo-methylated. In-depth analysis of DNA methylation profiles revealed 19 genes, out of 797 methylated genes associated with DN. HM exacerbates DN through altered methylation patterns at specific CpG sites. TSF group demonstrated significant reductions in glucose and urinary albumin/creatinine levels compared to the STZ + HM group. Furthermore, TSF shows potential as an effective treatment for DN by restoring aberrant methylation levels. The identification of specific genes associated with DN provides valuable insights into the underlying mechanisms of DN pathogenesis and offers potential therapeutic targets for further investigation.
Application of rigid bronchoscopy with endoscopic bronchial ultrasound-guided transbronchial node biopsy in pulmonary sarcoidosis
Authors: wan nansheng , meng han , wang zhongli , shi yu , Feng Jing 
Published: October 2023

Abstract

Background
Pathological examination of diseased lymph nodes is crucial for diagnosing pulmonary sarcoidosis, and obtaining quality histological specimens is important for improving the diagnosis rate. This study assessed the diagnostic rate and safety differences associated with performing endobronchial ultrasound-guided transbronchial node biopsy plus rapid on-site evaluation versus traditional endobronchial ultrasound-guided transbronchial needle aspiration plus rapid on-site evaluation for pulmonary sarcoidosis.
Methods
This retrospective study included data from 110 patients diagnosed with pulmonary sarcoidosis after undergoing rigid bronchoscopy combined with endobronchial ultrasound to obtain hilar or mediastinal lymph node specimens between March 2016 and May 2022. A rapid on-site evaluation was used to evaluate all intraoperative samples.
Results
Diagnostic rates of pulmonary sarcoidosis via endobronchial ultrasound-guided transbronchial node biopsy and endobronchial ultrasound-guided transbronchial needle aspiration were 94.0% (63/64) and 72.1% (31/43; relative risk = 1.30, 95% confidence interval: 1.07–1.59; P = 0.002), respectively. Regarding intraoperative safety, four (9%) patients in the endobronchial ultrasound-guided transbronchial needle aspiration group and five (7%) patients in the endobronchial ultrasound-guided transbronchial node biopsy group had intraoperative airway bleeding without intervention (relative risk = 1.02, 95% confidence interval: 0.91–1.15; P = 0.74). There were no other between-group differences in postoperative adverse effects.
Conclusions
Compared with traditional endobronchial ultrasound-guided transbronchial needle aspiration combined with rapid on-site evaluation, endobronchial ultrasound-guided transbronchial node biopsy combined with rapid on-site evaluation is of increased diagnostic value when assessing mediastinal or hilar lymphadenopathy suspected to be pulmonary sarcoidosis. Further, no significant differences in intra- or postoperative complications and adverse reactions were observed.
Pathogenic variants in human DNA damage repair genes mostly arose in recent human history
Authors: Zhao Bojin , Li Jiaheng , Sinha Siddharth , Qin Zixin , Kou Si Hoi , Xiao Fengxia , Lei Huijun , Chen Tianhui , Cao Wenming , Ding Xiaofan , Wang San Ming 
Published: October 2023

Abstract
Background
Genome stability is maintained by the DNA damage repair (DDR) system composed of multiple DNA repair pathways of hundreds of DDR genes. Germline pathogenic variation (PV) damages function of the affected DDR genes, leading to genome instability and high risk of cancer. Knowing evolutionary origin of human DDR PV is essential to understand the etiology of human cancer. However, answer to the issue remains largely elusive. In the study, we analyzed evolutionary origin for the PVs in human DDR genes.
Methods
We collected 169 DDR genes by referencing various databases, analyzing cross-species genomic data using the phyloFit program of the PHAST package, with visualization using GraphPad Prism software and the ggplot module. Ancient and modern human DDR PV detection and comparison using tools such as SAMtools and variant annotation databases. Evolution selection studies across 20 vertebrates used CodeML in PAML for phylogenetic analysis, and the MEGA were used for sequence alignment. We created an open-access database using a LAMP stack and performed statistical analyses using Pearson correlation and Kruskal-Wallis tests.
Results
Through phylogenic analysis, we ruled out cross-species conservation as the origin; using archeological approach, we identified rich DDR PVs shared between modern and ancient humans, which were mostly dated within the last 10,000 years. We observed similar pattern of quantitative PV distribution between modern and ancient humans. We also detected a group of ATM, BRCA2 and CHEK2 PVs shared between human and Neanderthals.
Conclusions
Our study reveals that human DDR pathogenic variation mostly arose in recent human history, and human high cancer risk caused by DDR PVs can be a by-product of human evolution.