bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of Heterosis in Photosynthetic Traits in F1 Generation of Sorghum Hybrids and Relationship with Yield Traits
Authors: Zhao Renjie , Li Yueqiao , Xu Chen , Zhang Zhian , Zhou Ziyang , Zhou Yihan , Qi Zexin 
Published: October 2023

Abstract
Heterosis plays an important role in improving crop yield. Understanding the expression of heterosis for photosynthetic physiological traits in sorghum is important for breeding sorghum varieties with strong hybrid dominance. In this study, six sorghum restorer lines and six sorghum sterile lines and their thirty-six hybrid combinations formulated according to the NCII incomplete double-row hybridization method were used as materials, and the performance of hybrids in the F1 generation of hybrids in terms of hybridization in leaf photosynthesis-related parameters and carbon metabolism-related enzymes and their correlation with yield traits were analyzed at the flowering stage. The results showed that net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), apparent leaf meat conductance (AMC), ribulose-1,5-bisphosphate (RuBP) carboxylase, phosphoenolpyruvate (PEP) carboxylase, and sucrose phosphate synthase (SPS) of hybrid sorghum showed significant high-parent heterosis, while inter-cellular carbon dioxide concentration (Ci), water use efficiency (WUE), and sucrose synthase (SuSy) mainly showed negative heterosis, a few showed high-parent heterosis. Thousand grain weight (TGW), grain weight per spike (GWPS) and dry matter content (DMC) all showed high high-parent heterosis, with the highest value of 82.54% for thousand grain weight (TGW). Meanwhile, Pn was positively correlated with Tr, Ci, Gs, RuBP carboxylase, PEP carboxylase, GWPS, TGW, and DMC, which indicated that Tr, Ci, and Gs could assist in the screening of high-photosynthesis sorghum varieties and that Pn could assist in the screening of carbon-efficient sorghum varieties, and that Pn was closely related to yield. In summary, the F1 generation of sorghum hybrids has shown significant heterosis at anthesis. Combined with the field performance, the Pn at anthesis can be used as a reference for early prediction of the yield potential of the F1 generation of sorghum hybrids and screening of carbon-efficient sorghum varieties.
SYNGR2 plays a tumor-promoting role in lung adenocarcinoma through PI3K-AKT signaling pathway
Authors: Liu Jiakun , Luan Yanchao , Han QingSong , Zhao Wei 
Published: October 2023

Abstract
Objectives
To analyze the expression level of SYNGR2 in lung adenocarcinoma, explored its prognostic and diagnostic value, and preliminarily discussed its mechanism of action.
Methods
The diagnostic value was assessed by generating the ROC curve using SYNGR2 expression data. COX regression and correlation analysis were conducted to establish its association with clinical features. Additionally, immunohistochemical staining was performed on samples from 20 patients with lung adenocarcinoma (LUAD) to validate the observed differences in expression levels. Furthermore, silencing of SYNGR2 in LUAD cells demonstrated inhibition of proliferation, invasion, migration, and colony formation abilities. Moreover, GO and KEGG analyses along with PPI analysis were employed to preliminarily investigate the underlying mechanism of SYNGR2 in lung adenocarcinoma.
Results
The results demonstrated an upregulation of SYNGR2 which was associated with shorter overall survival (OS) and progression-free survival (PFS). Furthermore, the receiver operating characteristic (ROC) curve analysis revealed a robust diagnostic value for SYNGR2. Additionally, the SYNGR2 gene exhibited a strong association with the PI3K-AKT signaling pathway.
Conclusions
SYNGR2 plays a tumor-promoting role in lung adenocarcinoma and may act through PI3K-AKT signaling pathway.
Electroantennographic and Behavioral Responses of Bactrocera cucurbitae (Coquillett) to Volatile Compounds of Luffa acutangular L
Authors: wangjingjing wangjingjing , yaTian Zhen , Zhou Yong ping , Yang Jin fang , Gao Xuyuan , Chen Hong song , Ma Wei hua , Zhou Zhong shi 
Published: October 2023

Abstract
The melon fly, Bactrocera cucurbitae (Coquillett), is an invasive pest of fruits and vegetables and. Melon fly attractants have been used in control strategies to reduce pest levels and have gained widespread attention in recent years. Previous studies have shown that some host fruits are attractive to melon flies; however, few have investigated the chemical compounds responsible for their attraction. In this study, volatile compounds from Luffa acutangula L. were isolated and identified using dynamic headspace adsorption and gas chromatography-mass spectrometry (GC-MS), and their effects on B. cucurbitae were investigated using electroantennography (EAG) and behavioral tests (trap assays). Chemical profiling identified 21 compounds in ridge gourds, with stearyl alcohol, methyl myristate, phenyl acetate, and methyl stearate being the major components. The EAG results revealed that 12 compounds elicited antennal responses in B. cucurbitae, and the differences in antennal responses between male and female B. cucurbitae adults caused by heptanal, phenyl acetate, p-xylene, alpha-pinene, and 1-octadecene were significant. Moreover, in the behavioral experiment, the EAG-active compounds methyl isovalerate, methyl myristate, 1-octadecene, methyl laurate,heptanal, and 1-decanol had either attractive or repellent effects on B. cucurbitae. Our findings provide a theoretical basis producing repellents or attractants for effective green control of B. cucurbitae.
The Magnesium Depletion Score is associated with increased likelihood of kidney stone disease among female adults
Authors: Xu Ying , Qin Yingyi , Lu Hongtao , Liu Lulu , Huang Weiyan , Huang Anwen , Ye Yufei , Shen Hui , Guo Zhiyong , Chen Wei 
Published: October 2023

Abstract
Object: The association between magnesium depletion score (MDS) and kidney stone disease (KSD) remains unknown. This study was designed to investigate the association of MDS with KSD in adults. Methods: A total of 19,654 participants were enrolled from the National Health and Nutrition Examination Surveys (NHANES). The MDS was calculated by assessing four aspects, including alcohol assumption, renal function, and use of diuretics and proton pump inhibitor (PPI). Multivariable logistic regressions were performed to explore the associations between MDS and the prevalence of KSD. Linear correlations were conducted explore the relationship of testosterone with MDS. Results: In the multivariable logistic regressions with full adjustment for confounding variables, the odds ratio of MDS associating with KSD was 1.28 (95% CI: 1.04–1.58, P = 0.022) in total population, and 1.70 (95% CI: 1.16–2.50, P = 0.007) in female participants. Besides, compared to the lowest MDS, the highest MDS was associated with a lower testosterone (β = -11.548, P = 0.001) after full adjustment in non-menopause women. Conclusion: This study highlighted a positive correlation of high MDS with KSD in female population, which may be associated low level of serum testosterone.
Participatory diagnosis for the management of degraded forest areas in the Sierra de Guadalupe State Park
Authors: Valencia-Trejo Guadalupe Montserrat , Lara-Bueno Alejandro , Cetina-Alcalá Victor Manuel , López-López Miguel Ángel , Ramírez-Herrera Carlos 
Published: October 2023

Abstract

Participatory diagnosis is the methodological process through which society participates in order to undertake and achieve sustainable development with the contribution of science and collective knowledge. The objective of this research is to analyze the current situation of the Sierra de Guadalupe State Park (SGSP) from the natural, historical, economic, and social aspects, through participatory research and thus influence the selection of trees and shrubs of multiple uses for reforestation that have the potential to establish agroforestry and silvopastoral systems. This study was carried out with the help of the following tools that are commonly used in a participatory diagnosis: SWOT analysis, timeline, and identification and classification of natural resources. In order to determine the current problems of the state park (SGSP), the "problem tree" method was used. To identify possible solutions to this problem, a collaborative workshop was conducted in which negative conditions were assigned to positive actions. In addition, 141 semi-structured interviews were conducted with relevant actors in the society using the "snowball" methodology. Field visits were also conducted to complement the information. A total of 72 and 44 species of wild fauna and flora, respectively. The priority forest species considered to initiate the restoration actions were: Prosopis laevigata, Vachellia schaffneri, and Quercus rugosa. The shrub species were: Opuntia tomentosa, Opuntia hyptiacatha, and Agave salmiana. In conclusion, with this study, reliable technical knowledge was generated with the participation of society, which will be used to carry out actions of protection, restoration, conservation, and productive reconversion.
Long-term humoral and cellular responses elicited by Gam-COVID-Vac (Sputnik V) vaccine in hemodialysis patients versus non-renal subjects: a prospective cohort study
Authors: Parshina Ekaterina , Zulkarnaev Alexey , Tolkach Alexey , Ivanov Andrey , Kislyy Pavel 
Published: October 2023

Abstract
Background
we aimed to assess long-term immunogenicity of the recombinant adenovirus 26 and 5 vector-based COVID-19 vaccine Gam-COVID-Vac (Sputnik V, developed by N.F. Gamaleya National Research Centre, Russia) in patients receiving maintenance hemodialysis compared to healthy subjects.
Methods
a prospective cohort study included patients treated with maintenance hemodialysis (n = 23) and healthy volunteers (n = 28). The levels of anti-severe acute respiratory syndrome coronavirus-2 specific IgG as well as specific T-cell responses were quantified in all participants at two time points: 1 and 6 months after complete vaccination. All participates were adults, had been vaccinated twice with Gam-COVID-Vac and had no prior history of confirmed COVID-19.
Results
In both groups, IgG levels decreased from month 1 to 6, however, antibodies did not decline more rapidly in the hemodialysis group (analysis of variance p = 0.7214 for the “time × group” interaction, non-adjusted model). At the end of the study, 48% of non-hemodialysis and 67% of hemodialysis participants showed T-cell positivity. T-spot counts dropped over time in non-hemodialysis controls, but not in hemodialysis subjects (p = 0.008 and p = 0.18, respectively).
Conclusions
Patients receiving hemodialysis maintain significant long-term humoral response after Gam-COVID-Vac vaccination, which is comparable to that in subjects with normal kidney function. Cellular response turned up to be more sustained over time in hemodialysis group.
Healthcare workers’ attitudes and factors affecting sexual and reproductive health services to adolescents in refugee settlements, western Uganda
Authors: Mambo Simon Binezero , Lodhi Moazzam Mohiuddin , Abalinda Mary Gorret , Asiimwe Isa , Abdulkarim Abubakar , Bizimana Gloria Neema , Mbina Solomon Adomi , Shinkafi Tijjani Salihu , Ibrahim Umar 
Published: October 2023

Abstract
Introduction
Adolescent sexual and reproductive health care needs are rising in both low and middle-income countries. This need is even higher in refugee settlements. This study aimed to assess the healthcare workers’ attitudes and the factors affecting adolescent sexual and reproductive health care in the Nakivale, Kyaka II, and Rwamwanja refugee settlements.
Methods
This was a cross-sectional quantitative study in which health workers from public and private health facilities from three randomly selected refugee communities in western Uganda responded to a questionnaire. The questionnaire collected information on services provided, the attitudes towards each of the services and the factors that hindered the provision of these services. Binary logistic regression was done to assess the baseline characteristics associated with provision of these services using SPSS version 26.
Results
Of the 386 medical professionals enrolled, 194(50.3%) were females with a mean age of 30.9 years (SD = 6.9). The services that were most commonly offered were contraception counselling/provision (81.3%), HIV prevention/care (79.0%), sexually transmitted infections prevention (78.5%) and comprehensive sexuality education provision (75.1%). The least offered services were safe abortion care (40.9%) and harmful traditional practice prevention (39.9%). The good attitudes were highest towards comprehensive sexuality education and antenatal, intrapartum plus postnatal care while the bad attitudes were highest toward safe abortion care. Inadequate training, age, residence, education level, designation and type of facility had a significant association with offering of the different services (P < 0.05 for all at multivariate level of analysis).
Conclusion
More efforts are still required toward provision of safe abortion care and harmful traditional practices prevention. This will be achieved by providing training which will be vital in improving knowledge and attitude toward these services. The training should be more focused on the older professionals and non-councilors.
Biological effects of Lippia alba essential oil against Anopheles gambiae and Aedes aegypti
Authors: Coulibaly Fangala Hamidou , Rossignol Marie , Haddad Mohamed , Carrasco David , Azokou Alain , Valente Adeline , Ginibre Carole , Koné Mamidou Witabouna , Chandre Fabrice 
Published: October 2023

Abstract

Background the management of mosquito resistance to chemical insecticides and the biting behaviour of some species are motivating the search for complementary and/or alternative control methods. The use of plants is increasingly considered as a sustainable biological solution for vector control. The aim of this study was to evaluate the biological effects of the essential oil (EO) ofLippia albaagainstAnopheles gambiaeandAedes aegyptimosquitoes. Methods phytochemical compounds were identified by GC-MS. Knockdown and mortality were determined according to the WHO test tube protocol. Contact irritancy was assessed by observing the movement of mosquitoes from a treated WHO tube to a second untreated tube. Non-contact repellency was assessed using a standardised high-thtoughput screening system (HITSS). Blood meal inhibition was assessed using a membrane feeding assay treated with EO. Results the EO was identified as the citral chemotype (geranial + neral) and it is composed mainly of geranial (26.79%), neral (19.07%) and geraniol (14.52%). The essential oil gave 100% KD60 in both species at a concentration of 1%. Mortalities of 100% were recorded withAn. gambiaeandAe aegyptiat concentrations of 1% and 5% respectively. The highest proportions of females escaping during the contact iritancy test were 100% forAn. gambiaeat 1% concentration and 94% forAe.aegyptiat 2.5% concentration. The 1% concentration produced the highest proportions of repelled mosquitoes in the non-contact repellency tests: 76.8% (An. gambiae) and 68.5% (Ae. aegypti). The blood meal inhibition rate at a dose of 10% was 98.4% inAe.aegyptibut only 15.5% inAn. gambiae. Conclusion the citral chemotype ofL. albaEO has highly interesting biological effects in both species that make it a potentially good candidate for its use in mosquito control. The results obtained in this study encourage the further evaluation ofL. albaessential oils from other localities and of different chemotypes.
Single-Cell Sequencing Unravels Heterogeneity in Annulus Fibrosus Cells and Highlights PRG4 as a Potential Marker in Disc Degeneration
Authors: LU JIAWEI , Guo Kai , Guo Youfeng , Wang Feng , Zeng Jianhua , Huang Yufeng , Wu Desheng 
Published: October 2023

Abstract
Background: Intervertebral disc degeneration is characterized by the rupture of annulus fibrosus (AF) structures, precipitating nucleus pulposus herniation. Ensuring robust AF healing is pivotal for optimal disc repair. Incomplete or delayed AF healing can culminate in recurrent herniations following surgical interventions. While the reactive nature of the AF to degeneration is acknowledged, the molecular and cellular underpinnings remain undefined.
Methods: We embarked on a comprehensive exploration of the degenerative trajectory by analyzing single-cell sequencing datasets from both degenerated and normative AF specimens. The human AF samples were also analyzed. The overarching aim was to delineate differential cellular phenotypes and gene expression patterns emblematic of each state.
Results: Our comparative analyses unveiled distinct, highly proliferative cellular subsets uniquely prevalent in degenerated discs. Intriguingly, these cellular subsets were hallmarked by an augmented expression of the PRG4 gene, a well-established marker encoding the lubricative molecule, Lubricin. Subsequent assays reaffirmed a significant correlation between Lubricin expression (as dictated by PRG4) and the propensity for AF degeneration, reinforcing our initial observations of elevated PRG4 transcription in degenerative states.
Conclusions: This study elucidates previously unrecognized cellular landscapes within degenerated AF and underscores the pivotal role of Lubricin in this context. Such revelations furnish a foundation for conceptualizing targeted therapeutic interventions, aiming to ameliorate AF ruptures and their sequelae.
A Preliminary Study for The Development and Psychometric Properties of Secondhand Smoke Exposure Assessment Tools
Authors: Noormohammadi Robab , Samadi Amirmohammad , Maleki Azam 
Published: October 2023

Abstract
Background
The exposure of non-smokers to tobacco smoke is known as secondhand smoke (SHS) exposure, which can significantly affect their health. Due to the unavailability of a standardized questionnaire to assess SHS exposure, this study aimed to design Psychometric Properties of SHS Exposure Assessment Tools.
Methods
The present study was an assessment tool study conducted on 160 adults with SHS exposure who were citizens of Zanjan, Iran, in 2020. In the first step, we carried out a literature review for creating tool items to assess SHS exposure. The psychometric properties steps include face validity, content validity, construct validity and reliability. Exploratory Factor Analysis with the Principal component factor analysis method was used to test the construct validity of the scale. The reliability of the tools was tested using Cronbach’s alpha coefficient.
Results
The KMO index of adequacy was measured to be 0.822 that more than 0.7, indicating the adequacy of cases for factor analysis. The questionnaire was approved with four factors and 26 items which explained a cumulative variance of 0.57%. The face validity of the questionnaire was evaluated; all items had an impact score above 1.5. Also, the content validity ratio and the content validity index were both adequate. Finally, the reliability of the questionnaire was approved based on Cronbach’s alpha coefficient of 0.88.
Conclusion
In the primary assessment validity and reliability of tools were adequate for the assessment of SHS exposure in adults. Further studies are suggested to assess the specificity and sensitivity of this tool.
Hydrocolpos causing bowel obstruction in a preterm newborn: A case report
Authors: Jouza Martin , Rejdova Ingrid , Cintula Lukas , Jouzova Anna , Jabandziev Petr 
Published: October 2023

Abstract

Background:Hymenal atresia is the most common congenital defect of the female urogenital tract. The spectrum of clinical manifestations is broad, ranging from mild cases undiagnosed until adolescence to severe cases of giant intraabdominal masses. The most common complication of hydrocolpos is bladder compression, resulting in obstructive uropathy and hydronephrosis.
Case presentation: We present here the case of a preterm neonate who was admitted to the surgical neonatal intensive care unit for bowel obstruction. The baby did not appear septic or unwell, a small amount of meconium passed frequently, and no bilious gastric residuals occurred. Based on these findings, acute abdominal obstruction was doubtful, and the surgeon chose a conservative (watch and wait) approach. Subsequently, we performed abdominal ultrasound and magnetic resonance imaging based on unclear information about a suspicious abdominal mass raised by the gynecologist shortly before the emergency C-section. The final diagnosis was congenital hydrocolpos due to hymenal atresia. The pediatric gynecologist indicated an incision of the imperforate hymen under general anesthesia. The incision resolved abdominal distention as well as the bowel obstruction.
Conclusion: The presentation of hydrocolpos was not typical (no bulging in the vaginal introitus) in our case, and clinical symptoms implied acute bowel obstruction shortly after birth.
The surgeon chose a conservative (watch and wait) approach as the baby did not appear unwell on the second day of life. Fortunately, diagnostic laparotomy was not required as the next step in bowel obstruction management. All clinical symptoms resolved after a minor surgical procedure.
Relationship between mechanical ventilation at birth and respiratory tract infections within one year of follow-up in preterm infants
Authors: Zhou Cailing , Wang Linghua , Ye Meiling , Lei Shuyao , Wang Lizhen , Zhang Meixian 
Published: October 2023

Abstract
The purpose of the study was to investigate the relationship between mechanical ventilation (MV) at birth and respiratory tract infections (RTIs) in premature infants during later stages of life. We recruited 132 premature infants including 81 received mechanical ventilation and 51 non-mechanical ventilation (non-MV) at birth during their hospital stay, who were regularly followed up in the outpatient department of premature infants after discharge from the Department of Neonatology between March 2021 to February 2023. MV group was subdivided into invasive mechanical ventilation (IMV) group and non-invasive mechanical ventilation (NIMV) group. Primary outcome was respiratory tract infections within the first year of life. The incidence of respiratory tract infection in the MV group was similar with that in the non-MV group(82.7% vs 80.4%, p = 0.736). After adjusting for gestational age (GA), birth weight, twins, mode of delivery, neonatal respiratory distress syndrome, Apgar score and bronchopulmonary dysplasia, mechanical ventilation was found to be not an independent risk factor for RTIs (NIMV group vs. non-MV group: OR 1.886, 95%CI 0.253–14.258, p = 0.536; IMV group vs. non-MV group, OR 1.553, 95%CI 0.219–11.041, p = 0.660).
Conclusions: Mechanical ventilation at birth did not increase the risk of respiratory infections in premature infants within one year after birth.