bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential expression and prognostic value of proliferation markers in different histological types of ovarian cancer
Authors: wang yurong , li xia 
Published: December 2023

Abstract
Purpose We will assess the expression of the Ki67 antigen in different histological types ofepithelial ovarian cancer and its correlation with pathological and clinical characteristics,and explore the clinical value of Ki67 antigen as a proliferation marker for diagnosis and prognosis.
Methods The GEPIA and GEO databases was used to assessed the mRNA expression of MKI67 in ovarian cancer. The expression of MKI67 in both normal and cancerous ovarian cells was assessed using qRT-PCR. Immunohistochemical staining was detected the expression of Ki67 protein in 104 ovarian cancers,general information was gathered,such as the age of each patient to analyze the connection between the expression of Ki67 and the clinicopathological features of the patients. Kaplan-Meier analysis examines the relationship between ovarian cancer patients' prognosis and Ki67 expression.
Results Ki67 was overexpressed in carcinoma ovarian cells and tissues compared with normal cells and tissues. Ki67 expression was significantly associated with FIGO stage (P<0.001), histologic subtype (P<0.001), involvement of unilateral/bilateral(P=0.007), and presence of lymph node metastasis(P=0.014). However, there was no association with other prognostic variables such age,tumor nature,or serum CA125. The expression of Ki67 was significantly different between serous carcinoma and endometrioid carcinoma (P=0.007) 、clear cell carcinoma (P <0.001). Moreover, its expression was significantly different between FIGO stage III and stages I (P=0.002) 、stage II (P=0.008). According to Kaplan-Meier analysis,the 3-year survival rate and PFS in the Ki67 high expression group were considerably poorer than in the low expression group (P<0.05).
Conclusions In the tissues of ovarian cancer, Ki67 was highly expressed, and its expression levels was closely correlated with clinicopathological features including tumor FIGO stage, histologic subtype, unilateral / bilateral involvement, and lymph node metastasis. Patients with high expression have a reduced survival rate and a worse prognosis. Inactivation of the proliferation marker Ki67 will lead to proliferative cell-specific death, therefore maybe a potential strategy for ovarian cancer.
Attitude, awareness, and understanding of Artificial Intelligence AI among medical and dental students in Jordan: a cross-sectional study
Authors: Abdallat Mahmoud , AlSamhori Jehad Feras , AlSamhori Abdel Rahman Feras , Shnekat Hala Hayel , AlSamhori Ahmad Feras , Abdallat Shahem , Murshidi Rand 
Published: December 2023

Abstract
Introduction:
The rapid growth of AI has transformed various sectors, including healthcare. AI aids medical diagnoses and treatment planning, but challenges such as privacy and ethical concerns exist. Understanding AI is crucial for medical and dental students in Jordan to enhance patient care. This study examines students' attitudes, awareness, and understanding of AI to inform effective education and better healthcare practices.
Methods:
A cross-sectional study was conducted in Jordan among medical and dental students from Nov 2022 to Mar 2023. Data were analyzed using descriptive statistics, Mann-Whitney U test, Kruskal-Wallis tests, and Spearman's correlation. Ethical approval was obtained, and SPSS was used for analysis.
Result:
A survey of 800 medical and dental students in Jordan revealed significant variations in AI awareness, understanding, and attitudes. Notably, 54.6% were female, average age 19.81. Only 38.8% had tech family background. 35.0% used social media for AI info. 28.0% believed AI would improve medical diagnosis. Correlation showed positive link between AI awareness and understanding (r: 0.491, p < 0.001).
Conclusion:
The younger generation of medical and dental professionals’ views AI as a collaborator in their practice. Strong interest and active engagement in AI-related courses highlight the importance of integrating AI education into medical curricula for innovative healthcare practices.
Physicians and midwives' perceptions and experiences of sexual health and right education and its influence on promoting women's empowerment: a qualitative content analysis study
Authors: Ghorbani Faeze , Latifnejad Robab , Khadivzadeh Talat , Mirzaii khadijeh 
Published: December 2023

Abstract

Background: Training of sexual assertiveness, which is the knowledge and skill of defense of sexual rights and sexual identity, would enhance marital satisfaction and adjustment. It is necessary to have a deep understanding of this concept in order to achieve strategies to promote sexual assertiveness to empower women. The purpose of this study was to explore perceptions and experiences of midwives and physicians towards the concept of sexual assertiveness training and its promotion in line with women's empowerment.Methods: This qualitative study was conducted from July to November 1401 in the hospitals and health centers of Mashhad, Iran. The data was collected by conducting semi-structured and individual interviews (telephone and virtual) by 14 participants, who were selected through purposive sampling until data saturation was reached. Data analysis was done using conventional content analysis with MAXQda software.Results::Three main categorieswas emerged from analysis of qualitative data including: 1) the concept of sexual assertiveness (the meaning of sexual rights in life, the effects of sexual rights), 2) complications of sexual rights violation (psychological side effects, physical side effects) and 3) strategies for empowering women (not having a gender bias on women, participation of women in social life, comprehensive sex education) was obtained.Conclusion:In order to empower women in sexual rights, midwives and physicians should use strategies such as comprehensive sex education for women, and also eliminating the gender bias on women's issues and encouraging women to participate and negotiate in their sexual life.
Application of exercise therapy in patients with chronic kidney disease-induced muscle atrophy: A scoping review
Authors: Yin Jiawei , Zhang Xiaotu , Wang Zilin , Sun Xuefeng , Qu Zihan , Song Yuqing , Zhang Hongshi 
Published: December 2023

Abstract

Objective: To conduct a systematic analysis of existing research on the application of exercise intervention in the nursing care of patients suffering from chronic kidney disease (CKD)-induced muscle atrophy and identify the specific exercise modalities, contents, outcome indicators, and application effects associated with this intervention.
Methods:According to the method of a scoping review, nine databases (Cochrane, PubMed, EMBASE, Web of Science, ProQuest, Ovid, CNKI, Wanfang Data, and VIP) were searched until September 28, 2023. The included literature was screened, summarized, and analyzed.
Results: A total of 20 pieces of literature were included. Some types include aerobic exercise, resistance exercise, and aerobic combined resistance exercise. The exercise intensity primarily falls within the mild to moderate range, with a recommended frequency of 2-3 times a week, lasting 30-60 min each time. The types of outcomes encompassed in this study include body composition, functional testing, strength measurements, laboratory examinations, cardiopulmonary function assessments, and patient-reported outcomes. To varying degrees, exercise intervention positively impacts the subjects' physical activity ability, body composition, and skeletal muscle status. Currently, resistance training is the primary type of intervention used for preventing and treating CKD patients induced by muscle atrophy.
Conclusion: Exercise intervention can improve muscle strength, physical function, and quality of life in patients with CKD muscle atrophy. Therefore, patients should be fully informed of the effect of exercise intervention in the treatment of chronic kidney disease-induced muscle atrophy in future, so as to promote the standardized implementation of exercise intervention.
Research on the privacy protection technology of blockchain spatiotemporal big data
Authors: Zhou Bao , Zhao Junsan , Chen Guoping , Yin Ying 
Published: December 2023

Abstract

At present, the fast-paced development of big data technology and the Internet of Things has led to the generation of spatiotemporal data streams on a large scale. In this circumstance, data holders often choose the cloud storage of their data to alleviate with the pressure of local storage and computing overhead. However, this is a centralized mode of storage, and the data is susceptible to the risk of tampering and leakage in the absence of physical control over spatio-temporal big data. Therefore, a blockchain-based space-time big data privacy protection scheme is devised in this paper. To begin with, in order to ensure data privacy, the scheme adopts a storage mode in which the public chain are coordinated with the side chain. The former stores various information such as keys and security authentication parameters, while the latter stores private data. Then, the irreversible SHA256 algorithm is applied to authenticate the access nodes. Finally, AES and RSA hybrid encryption is performed to encrypt the collected spatio-temporal big data for the security of private data to be further enhanced. The security performance of the system is evaluated by comparing the success rate of attacks on the blockchain under different circumstances. Also, the system throughput of this scheme is analyzed through simulation, the results of which show that the service life of this system reaches a minimum of 13.79 years when 100 nodes are use. In this context, the system throughput reaches 1607 transactions/s, each node contains 1.2×107 blocks, and the time taken to query specific spatio-temporal data is less than 2 seconds.
Blood cadmium concentrations and risk of all-cause mortality among adults with chronic pruritic dermatoses: A prospective cohort study
Authors: Xiao Yu , Li Shan , Yang Wanying , Zhang Tianyi , Liu Chao 
Published: December 2023

Abstract
Cadmium (Cd) accumulates in the human body over time, posing significant health risks. This study focused on the relationship between blood Cd concentrations and all-cause mortality in adults suffering from chronic pruritic dermatoses (CPD). Data utilized in our study was collected from the National Health and Nutrition Examination Survey for the 2005–2006 cycle in the United States. To assess the hazard ratio (HR) for mortality, a weighted multivariable Cox regression analysis was conducted, considering age, sex, race, body mass index, waist circumference, hay fever, and allergy. The restricted cubic spline analyses were adopted to explore the dose-response association. Additionally, subgroup and sensitivity analyses were implemented to estimate the stability of the findings. In this study, 323 participants were collected from 10,348 individuals. Compared with the lowest quantile of blood Cd concentrations group, individuals in the highest quantile had an adjusted HR of 3.35 (95% CI: 1.46, 7.73) for all-cause mortality. The restricted cubic spline analyses of multivariate adjustment revealed a significant linear dose-response relationship between ln-transformed blood Cd concentrations (ranging from 0.46 to 3.96µg/g ) and the risk of all-cause mortality. In addition, no significant interactions were observed between strata variables and blood Cd (all Pinteraction > 0.05). Sensitivity analyses also yielded similar results to those obtained in our study. Cd exposure serves as a critical risk factor for all-cause mortality in adults suffering from CPD. Our data substantiate a linear relationship between blood Cd concentrations and mortality risk. These findings underscore the imperative for policy makers and the general public to mitigate Cd exposure, thereby reducing long-term adverse health outcomes.
NSUN7 gene polymorphisms increase the risk of neuroblastoma in Chinese children
Authors: Xu Yile , Zhang Weixin , Hu Yaofang , Guo Huiqin , Li Meng , Li Di , Lin Lei , He Jing , Miao Lei 
Published: December 2023

Abstract
Purpose
Neuroblastoma (NB) is the most common childhood solid tumor, exhibiting significant genetic variability. This study aims to assess the impact of NSUN7 gene polymorphisms (rs55690540 T > G, rs2437323 G > T, rs4861311 A > G, and rs11724316 T > C) on the susceptibility and prognosis of NB in a Chinese cohort. It focuses on elucidating the role of NSUN7 expression correlation with NB progression and outcomes, leveraging public databases and bioinformatics analysis.
Methods
A case-control study comprising 398 NB patients and 473 healthy controls was conducted, utilizing TaqMan assays for genotyping NSUN7 polymorphisms. Multivariate logistic regression determined the association between these polymorphisms and NB risk. The GTEx database was used to assess gene expression impact. Kaplan–Meier analysis and bioinformatics tools, including GSEA and KEGG pathway analysis, were employed to evaluate the prognostic implications of NSUN7 expression in datasets GSE49710 and GSE45547.
Results
Certain NSUN7 polymorphisms, particularly rs11724316 CC and rs2437323 TT genotypes, showed a significant association with increased NB susceptibility. Stratification analysis revealed age and stage-specific correlations. Elevated NSUN7 expression, correlated with poor prognosis in NB, was linked to significant metabolic shifts and involvement in key pathways like KRAS signaling, E2F targets, and G2M checkpoint.
Conclusion
NSUN7 polymorphisms emerge as potential biomarkers for NB susceptibility and prognosis. High NSUN7 expression is associated with adverse outcomes, highlighting its critical role in NB pathogenesis and as a promising target for therapeutic intervention. This study provides a foundation for future research in neuroblastoma genetics and treatment strategies.
Impact of Large-Scale Disasters on Breast Cancer Care: A Qualitative Analysis of Patient Experiences During the 2011 Triple Disaster in Fukushima, Japan
Authors: Kaneda Yudai , Ozaki Akihiko , Murakami Michio , Saito Hiroaki , Sawano Toyoaki , Yamashiat Erika , Gonda Kenji , Tachibana Kazunoshin , Ohtake Tohru , Tsubokura Masaharu , Ohira Hiromichi 
Published: December 2023

Abstract

Background Large-scale disasters can severely affect breast cancer care, leading to treatment disruption and delay. Although few empirical studies have explored these effects, analyzing patients’ narratives can help bridge this gap. This study aimed to explore treatment continuity and medical access among patients with breast cancer during the 2011 triple disaster using qualitative research methods. Methods This qualitative study focused on 21 patients with breast cancer diagnosed before the disaster and were affected by it. Upon receiving consent, semi-structured interviews were conducted, lasting between 20 min to 1 h. Inductive thematic analysis was used for data interpretation. Results Five key themes emerged in the present study, ranging from immediate response to long-term recovery: medical challenges faced after the disaster, family roles and burdens, information access and communication, mental health effects of disasters, and challenges faced during evacuation. These themes covered a range of challenges, including infrastructure damage, treatment disruptions, substantial constraints arising from familial roles that could overburden the family support, importance of reliable medical and nonmedical information sources, psychological impacts of the disaster, and unique difficulties experienced in evacuation shelters. Conclusions This analysis highlights the critical need for ongoing care and addresses the key challenges faced by breast cancer patients during disasters. Future research should aim to develop practical and prompt solutions to address these issues in disaster settings.
Fast recovery of COVID-19-induced acute respiratory distress syndrome after hyperbaric oxygen treatment and changes in endoplasmic reticulum (ER) stress response in peripheral monocytes – A randomized-controlled trial
Authors: Kjellberg Anders , Zhao Allan , Lussier Anna , Hassler Adrian , Al-Ezerjawi Sarah , Boström Emil , Catrina Sergiu-Bogdan , Bergman Peter , Rodriguez-Wallberg Kenny Alexandra , Lindholm Peter 
Published: December 2023

Abstract
Background: Many questions remain unanswered regarding the scientific understanding of SARS-CoV-2 and host interaction. There is an established need to develop anti-inflammatory agents with the potential to prevent or mitigate the development of severe COVID-19, targeting viral replication and/or the immune response. To address these unmet needs, we investigated the effects of oxygen treatment (HBOT) on clinical inflammatory markers, peripheral blood cells and transcriptional changes in patients with severe COVID-19.
Methods: In this pre-defined sub-study of a randomized controlled clinical trial, critical COVID-19 patients with moderate acute respiratory distress syndrome (ARDS) at one Swedish university hospital were enrolled and randomly assigned (1:1) to HBOT plus best practice or best practice (Control) and followed up on nine visits for 30 days. HBOT was administered with five treatments at 2.4 atmospheres absolute (ATA) for 80 min each, the first seven days. Clinical inflammatory markers, lymphocyte phenotyping with flow cytometry, and bulk RNA sequencing (RNAseq) were analyzed on peripheral blood monocytes (PBMCs).
Results: Between December 3rd 2020, and May 17th 2021, 23 patients were randomized, and 17 were analyzed. RNAseq revealed 791 differentially expressed genes in the HBOT group compared to 46 in the control group at Day 7 vs. baseline. Gene set enrichment analysis revealed unique decreases of the unfolded protein response (UPR) and inflammatory response in the HBOT group. The HBOT group recovered faster. National early warning score (NEWS) was lower in the HBOT group (ANOVA, F(8, 120) = 3.817, p < 0.001) and PaO2/FiO2 was higher in the HBOT group (Mixed effects model, F(8, 94) = 2.900, p < 0.01). The HBOT group had a shorter mean hospital length of stay (HLoS), 16 vs. 26 days (95.99% CI -16-0), p=0.045, saving ~300.000 USD.
Conclusions: Pathways related to viral-induced ER stress with downregulation of the UPR and inflammatory response in critical COVID-19 patients treated with HBOT was significantly different compared to controls and associated with faster recovery and reduced HLoS.
Trial registration: NCT04327505 (March 31, 2020) and EudraCT 2020-001349-37 (April 24, 2020)
Inborn error of WAS presenting with SARS-CoV-2-related multisystem inflammatory syndrome in children
Authors: Drago Enrico , Fioredda Francesca , Penco Federica , Prigione Ignazia , Bertoni Arinna , Zotto Genny Del , Bocca Paola , Massaccesi Erika , Lanciotti Marina , Moratto Daniele , Thurner Lorenz , Caorsi Roberta , Gattorno Marco , Volpi Stefano 
Published: December 2023

Abstract

Multisystem inflammatory syndrome in children (MIS-C) has been reported in patients with inborn errors of immunity (IEI), providing insights into disease pathogenesis. Here, we present the first case of MIS-C in a child affected by Wiskott-Aldrich syndrome (WAS) gene mutation, elucidating underlying predisposing factors and the involved inflammatory pathways. Genetic analysis revealed a frameshift truncating variant in the WAS gene, resulting in WAS protein expression between mild and severe forms, despite a clinical phenotype resembling X-linked thrombocytopenia (XLT). IL-1β secretion by LPS-stimulated peripheral blood mononuclear cells from patient during MIS-C was lower compared to healthy subjects but increased during follow-up. Conversely, the percentage ASC (apoptosis-associated speck-like protein containing a CARD) specks in the patient's circulating monocytes during the acute phase was higher than in healthy subjects. The type I interferon (IFN) score during MIS-C was normal, in contrast to the raised IFN signature measured far from the acute event. This case confirms the association of IEI with MIS-C, potentially linked to delayed immune responses to SARS-CoV-2. The XLT phenotype underlies a subclinical immunodysregulation involving the NLRP3 inflammasome and the type-I IFN response.
Fingertip Tactile Sensation via Piezoelectric Micromachined Ultrasonic Transducers with an Amplified Interface
Authors: Sone Junji 
Published: December 2023

Abstract

Tactile devices are often used in the field of robotics; however, the development of compact high-resolution tactile devices remains challenging. In this study, we developed a haptic device for force presentation using a DC motor and a tactile sensation device to simultaneously present haptic and tactile stimuli. A microelectromechanical system was selected to maintain the compactness of the tactile device. Piezoelectric micromachined ultrasonic transducers are known for high-power stimulation, and we selected lanthanum-doped lead zirconate titanate as the high-power amplified actuator. A finger mount structure that transfers force for amplifying ultrasonic waves was considered to combine acoustic pressure and aeroacoustics by attaching silicone rubber. The device was fabricated, and the performance of the tactile sensations was evaluated. The developed device uses the novel concept of combining acoustic pressure and aeroacoustics, and its compactness renders it suitable for wearable systems.
Exosomes from myoblasts induced by hypoxic preconditioning improved ventricular conduction by increasing Cx43 expression in hypothermia ischemia reperfusion hearts
Authors: Hu Tingju , Gao Hong , Duan Rui , Bai Xue , Huang Xiang , Yan Xu , An Li , Ma Yanyan , Chen Rui , Hong Sen , Gan Mi 
Published: December 2023

Abstract

Myocardial ischemia-reperfusion arrhythmia after cardiac surgery is common and seriously affects quality of life. Remote ischemic preconditioning can reduce the myocardial damage caused by severe ischemia. However, the underlying mechanism is not well understood. This study aimed to investigate the effects of exosomes derived from C2C12 mouse myoblasts after hypoxic preconditioning (HP) on ventricular conduction in hypothermic ischemia-reperfusion hearts. Myocardial ischemia-reperfusion model rats were established using the Langendorff cardiac perfusion system. Exosomes derived from normoxic (ExoA) and hypoxia-preconditioned (ExoB) C2C12 cells were injected into the jugular vein of the model rats. The time to heartbeat restoration, arrhythmia type and duration, and heart rate were recorded after myocardial ischemia-reperfusion. Conduction velocity on the surface of left ventricle was measured using a microelectrode array after 30 min of balanced perfusion, 15 min of reperfusion, and 30 min of reperfusion. Immunofluorescence and western blotting were performed to determine the distribution and relative expression of connexin 43 (Cx43). ExoB contained more exosomes than ExoA, showing that HP stimulated the release of exosomes. The IR + ExoB group showed faster recovery of ventricular myocardial activity, a lower arrhythmia score, faster conduction velocity, and better electrical conductivity than the IR group. ExoB increased the expression of Cx43 and reduced its lateralization in the ventricular muscle. Our study showed that exosomes induced by hypoxic preconditioning can improve ventricular myocardial conduction and reperfusion arrhythmia in isolated hearts after hypothermic ischemia-reperfusion.