bluuebunny/crossref_metadata_2025_split huggingface dataset:
A gene regulatory network critical for axillary bud dormancy directly controlled by Arabidopsis BRANCHED1
Authors: van Es Sam W. , Muñoz‐Gasca Aitor , Romero‐Campero Francisco J. , González‐Grandío Eduardo , de los Reyes Pedro , Tarancón Carlos , van Dijk Aalt D. J. , van Esse Wilma , Pascual‐García Alberto , Angenent Gerco C. , Immink Richard G. H. , Cubas Pilar 
Published: November 2023

Summary

The Arabidopsis thaliana transcription factor BRANCHED1 (BRC1) plays a pivotal role in the control of shoot branching as it integrates environmental and endogenous signals that influence axillary bud growth. Despite its remarkable activity as a growth inhibitor, the mechanisms by which BRC1 promotes bud dormancy are largely unknown.

We determined the genome‐wide BRC1 binding sites in vivo and combined these with transcriptomic data and gene co‐expression analyses to identify bona fide BRC1 direct targets. Next, we integrated multi‐omics data to infer the BRC1 gene regulatory network (GRN) and used graph theory techniques to find network motifs that control the GRN dynamics. We generated an open online tool to interrogate this network. A group of BRC1 target genes encoding transcription factors (BTFs) orchestrate this intricate transcriptional network enriched in abscisic acid‐related components.

Promoter::β‐GLUCURONIDASE transgenic lines confirmed that BTFs are expressed in axillary buds. Transient co‐expression assays and studies in planta using mutant lines validated the role of BTFs in modulating the GRN and promoting bud dormancy.

This knowledge provides access to the developmental mechanisms that regulate shoot branching and helps identify candidate genes to use as tools to adapt plant architecture and crop production to ever‐changing environmental conditions.
Rate‐dependent elevation of the capture threshold after implantation of a leadless pacemaker
Authors: Sano Makoto , Takahashi Naoyuki , Kaneko Yutaro , Narumi Taro , Naruse Yoshihisa , Maekawa Yuichiro 
Published: November 2023

AbstractThe procedural success in the implantation of cardiac electric devices depends on both the implanted position and the electric performance. The capture threshold and the pacing output affect the estimated battery longevity. In a case with a high capture threshold, recapture and reimplantation of a leadless pacemaker are commonly recommended. We experienced a case with the rate‐dependent elevation of the capture threshold following the implantation of a leadless pacemaker. The recognition of the rate‐dependency of the capture threshold and the acceptable programming could avoid the unnecessary recapture and reimplantation of that, avoiding the increase of procedural risks.
The Matter of Coincidence
Authors: Mooney Justin 
Published: November 2023

AbstractThe phasalist solution to the puzzle of the statue and the piece of clay claims that being a statue is a phase sortal property of the piece of clay, just like being a child is a phase sortal property of a human being. Some philosophers reject this solution because it cannot account for cases where the statue seems to gain and lose parts that the piece of clay does not. I rebut this objection by arguing, contrary to the prevailing view, that the piece of clay is not mereologically constant and might even be highly mereologically flexible.
ZmELF6‐ZmPRR37 module regulates maize flowering and salt response
Authors: Su Huihui , Cao Liru , Ren Zhenzhen , Sun Wenhao , Zhu Bingqi , Ma Shixiang , Sun Chongyu , Zhang Dongling , Liu Zhixue , Zeng Haixia , Yang Wenjing , Liu Yingpeng , Zheng Lingling , Yang Yuwei , Wu Zhendong , Zhu Yingfang , Ku Lixia , Chong Leelyn , Chen Yanhui 
Published: November 2023

SummaryThe control of flowering time in maize is crucial for reproductive success and yield, and it can be influenced by environmental stresses. Using the approaches of Ac/Ds transposon and transposable element amplicon sequencing techniques, we identified a Ds insertion mutant in the ZmPRR37 gene. The Ds insertion showed a significant correlation with days to anthesis. Further research indicated that ZmPRR37‐CR knockout mutants exhibited early flowering, whereas ZmPRR37‐overexpression lines displayed delayed flowering compared to WT under long‐day (LD) conditions. We demonstrated that ZmPRR37 repressed the expression of ZmNF‐YC2 and ZmNF‐YA3 to delay flowering. Association analysis revealed a significant correlation between flowering time and a SNP2071‐C/T located upstream of ZmPRR37. The SNP2071‐C/T impacted the binding capacity of ZmELF6 to the promoter of ZmPRR37. ZmELF6 also acted as a flowering suppressor in maize under LD conditions. Notably, our study unveiled that ZmPRR37 can enhance salt stress tolerance in maize by directly regulating the expression of ABA‐responsive gene ZmDhn1. ZmDhn1 negatively regulated maize salt stress resistance. In summary, our findings proposed a novel pathway for regulating photoperiodic flowering and responding to salt stress based on ZmPRR37 in maize, providing novel insights into the integration of abiotic stress signals into floral pathways.
Isolation of yellow vein mosaic virus (YVMV)‐resistant mutants of okra (Abelmoschus esculentus L.) through applied mutagenesis
Authors: Hazra Soham , Gorai Shouvik , Roy Sourav , Bose Suvojit , Hazra Pranab , Chattopadhyay Arup , Ali Md. Nasim , Jambhulkar Sanjay , Maji Anirban 
Published: November 2023

AbstractOkra, Abelmoschus esculentus (L.) Moench, being highly susceptible to yellow vein mosaic virus (YVMV) disease warrants its genetic improvement for resistance. Applied mutagenesis programme using two optimum doses of gamma radiation, namely, 350 Gy and 450 Gy radiation, was administered for the variety Pusa Sawani rated as excellent for fruit quality but highly susceptible to YVMV disease. Two selected putative mutant families, namely, 350//10///3‐9////28 and 450//66///2‐4////39, isolated in the M5 generation with slightly different plant morphology as compared to the parental genotype, Pusa Sawani, showed consistent resistance against YVMV disease. Upon evaluation in the M6 generation, eight morphological characteristics and five quantitative characteristics differed significantly among the mutants and Pusa Sawani. Proximate compositions and enzyme activity in leaf were significantly higher in the two YVMV‐resistant mutants. Disease screening under artificial inoculation in the insect proof cages confirmed YVMV resistance in these mutants. DNA fingerprinting further validated the alterations occurred in these two isolated mutants compared to the parental genotype. These mutants deserve due attention towards the development of YVMV‐resistant variety.
Bridging the gap in BASCULE syndrome: A retrospective case series of a recently described clinical entity
Authors: Reinhart Jacob P. , Kumar Anagha Bangalore , Casanegra Ana I. , Rooke Thom W. , Sartori‐Valinotti Julio C. , Tollefson Megha M. , Klaas Kelsey M. , Davis Dawn M. 
Published: November 2023

AbstractBackgroundBier anemic spots, cyanosis with urticaria‐like eruption (BASCULE) syndrome is a recently described entity with episodic urticarial lesions and white anemic halos on a background of erythrocyanosis, commonly affecting the lower extremities. Possible association with autonomic dysfunction remains poorly understood. Existing publications are limited, but the condition is suggested as highly underrecognized.ObjectiveTo further characterize clinical and epidemiologic data for BASCULE syndrome.MethodsWe performed an IRB‐approved retrospective chart review on patients with BASCULE syndrome evaluated at Mayo Clinic from April 2021 to November 2022.ResultsA total of 17 patients were identified (13 female, 4 male). Median age of onset was 12 years (range 9–17). Lower extremities were involved in all patients (17). Most patients were symptomatic with pruritus (8) or burning pain (8); three were asymptomatic. Triggers were standing (11), hot showers or hot environments (7), or no clear trigger (4). Autonomic dysfunction was present in 10 patients. Treatment responses were observed from propranolol (3) and high‐dose cetirizine (1).ConclusionNovel epidemiologic data from 17 pediatric and young adult patients with BASCULE syndrome further supports an association with autonomic dysfunction and suggests a higher prevalence than previously acknowledged.
Influence of post‐disaster evacuation on childhood obesity and liver dysfunction: The Fukushima Health Management Survey
Authors: Hosoya Mitsuaki , Nakano Hironori , Hashimoto Koichi , Ohira Tetsuya , Sakai Akira , Shimabukuro Michio , Yasumura Seiji , Ohto Hitoshi , Kamiya Kenji 
Published: November 2023

AbstractBackgroundAfter the Great East Japan Earthquake and subsequent Fukushima Daiichi Nuclear Power Plant accident in 2011, the Fukushima Prefectural Government launched a long‐term health management survey for the population of Fukushima. Results of the Comprehensive Health Check (CHC) showed that some children aged 6–15 years, who resided in the evacuation area at the time of the disaster, had obesity, hyperlipidemia, liver dysfunction, and/or renal dysfunction from as early as 2011. The aim of the present study was to determine the long‐term trend of obesity and hepatic enzyme abnormalities in Fukushima children.MethodsWe evaluated the changes in body mass index standard deviation score (BMI‐SDS), aspartate aminotransferase, alanine aminotransferase, and gamma‐glutamyl transpeptidase from 2011 to 2018.ResultsObesity (BMI‐SDS ≥ 2) was significantly associated with hepatobiliary enzyme abnormalities. The mean BMI‐SDS was significantly higher in 2011 after the disaster, but then soon showed a gradual decrease. The frequency of obesity did not increase significantly after the disaster. There were no significant differences in the prevalence of hepatobiliary enzyme abnormalities in the children aged 6–15 years of either sex from 2011 to 2018.ConclusionsIn the present study, we found that the increase in the mean BMI‐SDS after the disaster was temporary, suggesting that the frequency of obesity and liver dysfunction might not have been significantly influenced by the disaster.
The length of the 3′ UTR of the tomato torrado virus (ToTV) RNA1 affects virus accumulation in Solanum lycopersicum during mechanical passages from plant to plant
Authors: Budziszewska Marta , Wrzesińska‐Krupa Barbara , Wieczorek Przemysław , Obrępalska‐Stęplowska Aleksandra 
Published: November 2023

AbstractTomato torrado virus (ToTV), a member of the Torradovirus genus, primarily infects tomatoes. Previous analyses revealed high heterogeneity in the 3′ untranslated region (3′ UTR) of RNA1 of isolate ToTV‐Kra. In addition to the full‐length 3′ UTR RNA1 (a wild‐type, wt), four truncated versions (var2, var3, var4 and var5) were identified. Here, we investigated the biological importance of this phenomenon by assessing whether such defective genomes are infectious individually, and how the length of the 3′ UTR influences disease symptoms, virus transmission and viral RNA accumulation. Using the ToTVpJL‐Kra infectious clone, we introduced deletions corresponding to the known RNA1 defective variants and examined their impact on ToTV virulence and sap transmission ability. Viral RNA accumulation was assessed in agroinfiltrated tomatoes, as well as during serial passages. We found that all defective genomes were infectious and the length of the 3′ UTR of RNA1 influenced viral RNA accumulation. Tomatoes agroinfiltrated with var2, var3 or var5 showed the highest copy numbers of genomic RNAs. However, during serial passages, ToTVpJL‐Kra‐var1 and ‐var2 (six nucleotides shorter) showed limited sap transmission ability compared to the other variants, which persisted and replicated well in tomatoes. Plants treated with ToTVpJL‐Kra‐var3, ‐var4 and a mixture of var1–var5 showed the highest viral RNA accumulation, which was not associated with increased symptom severity in comparison to the other variants. Additionally, we identified a further sequence insertion in the 3′ UTR of var3 RNA1. This insertion could have occurred spontaneously or as a result of virus adaptation to the mode of transmission.
A high‐throughput analysis of high‐resolution X‐ray CT images of stems of olive and citrus plants resistant and susceptible to Xylella fastidiosa
Authors: Walker Nancy C. , Ruiz Siul A. , Ferreira Talita R. , Coletta‐Filho Helvecio D. , Le Houx James , McKay Fletcher Daniel , White Steven M. , Roose Tiina 
Published: November 2023

AbstractThe bacterial plant pathogen Xylella fastidiosa causes disease in several globally important crops. However, some cultivars harbour reduced bacterial loads and express few symptoms. Evidence considering plant species in isolation suggests xylem structure influences cultivar susceptibility to X. fastidiosa. We test this theory more broadly by analysing high‐resolution synchrotron X‐ray computed tomography of healthy and infected plant vasculature from two taxonomic groups containing susceptible and resistant varieties: two citrus cultivars (sweet orange cv. Pera, tangor cv. Murcott) and two olive cultivars (Koroneiki, Leccino). Results found the susceptible plants had more vessels than resistant ones, which could promote within‐host pathogen spread. However, features associated with resistance were not shared by citrus and olive. While xylem vessels in resistant citrus stems had comparable diameters to those in susceptible plants, resistant olives had narrower vessels that could limit biofilm spread. And while differences among olive cultivars were not detected, results suggest greater vascular connectivity in resistant compared to susceptible citrus plants. We hypothesize that this provides alternate flow paths for sustaining hydraulic functionality under infection. In summary, this work elucidates different physiological resistance mechanisms between two taxonomic groups, while supporting the existence of an intertaxonomical metric that could speed up the identification of candidate‐resistant plants.
Diagnosis and management of exuberant palatal pyogenic granuloma in a systemically compromised patient – Case report
Authors: Ventura José Victor Lemos , Vogel Jéssica de Oliveira , Cortezzi Ellen Brilhante de Albuquerque , de Arruda José Alcides Almeida , Cunha John Lennon Silva , Andrade Bruno Augusto Benevenuto de , Tenório Jefferson R. 
Published: November 2023

AbstractAimTo discuss the diagnosis and treatment of an exuberant oral pyogenic granuloma (OPG) in the palatal region in a systemically compromised patient.Methods and ResultsA 50‐year‐old woman presented with extensive and painless nodular mass that extended throughout the palatal region, with difficulty speaking, swallowing and spontaneous bleeding for 6 months. Her medical history showed poorly controlled type II diabetes mellitus and hypertension. The intraoral physical examination also revealed poor oral hygiene and periodontal disease. After clinical and radiographic evaluation, the presumptive diagnosis of OPG was made and complete excision of the lesion was performed. Local hemostatic measures were employed to control bleeding. Microscopic evaluation showed a lobulated lesion composed of many blood capillaries confirming the diagnostic hypothesis.ConclusionsIn summary, individuals with severe periodontal disease and systemic disorders may present exacerbated clinical presentations of OPG.
Understanding the challenges and opportunities of talking to children about race and racism in child‐facing institutions
Authors: Wilton Leigh S. , Sullivan Jessica , Albuja Analia F. , Perry Sylvia P. 
Published: November 2023

AbstractResearchers from a range of disciplines emphasize that effectively socializing children about race and racism is vital to promoting positive outcomes, such as disrupting the development of racist attitudes and beliefs. While parents and guardians influence children's racial attitudes and beliefs, children also learn about race and racism from many other important adults in their lives, such as teachers, community leaders, and librarians. Yet race‐related topics are largely absent or underdeveloped from child‐facing programming, policies, and procedures. In this paper, we describe three major barriers to effectively socializing children about race and racism within child‐facing institutions like libraries, museums, and schools: (1) adults’ (often inaccurate) beliefs about children's social and cognitive race‐related development, (2) adults’ knowledge and comfort discussing race and racism with others, and (3) social norms that minimize explicit discussion of race and racism in institutional and interpersonal contexts. To contextualize these barriers, we address how children can process and reason about race across childhood and outline the evidence‐based benefits of socializing children about race and racism inside and outside the home. Finally, we provide recommendations aimed at translating this research to child‐focused spaces and provide general guidelines to consider when implementing such practices in their own spheres of influence. In sum, we argue that effective socialization about race and racism benefits all children and can (and should!) be achieved successfully outside of the home; and that adults who interact with children must interrogate their own beliefs, biases, and perspectives, work to develop cultural competence, and invest in and continually reassess practices and policies to facilitate effective socialization about race and racism.
Transient Horner's syndrome in horses following cervical plexus local anesthesia
Authors: Pearce Duncan J. , Raffetto Jennifer , Vallance Stuart A. 
Published: November 2023

AbstractObjectiveTo report the development of transient Horner's syndrome in horses following local anesthesia of the cervical plexus.AnimalsA total of 37 horses.Study designObservational retrospective short case series.MethodsMedical records of cases undergoing ultrasound‐guided cervical plexus anesthesia for standing prosthetic laryngoplasty at a single referral institution were evaluated (2019–2020).ResultsFive of 37 horses (14%) developed transient signs of Horner's syndrome postoperatively. Obvious clinical signs began 2 to 5 h following local anesthesia and persisted for two to 4 h. Profuse unilateral sweating of the head and upper neck was the most apparent clinical sign.ConclusionTransient clinical signs of Horner's syndrome were seen following unilateral local anesthesia of the cervical plexus. Long‐term adverse sequelae were not observed.
Unveiling the spatiotemporal evolutionary patterns of eriophyoid mites in China
Authors: Li Ni , Hu Yue , Yao Liang‐Fei , Gao Wei‐Nan , Xue Xiao‐Feng 
Published: November 2023

AbstractEriophyoid mites are among the most species‐rich superfamilies (Eriophyoidea) in the Acari, consisting of over 5000 named species. Although they exhibit a geographically uneven worldwide distribution, their regional spatiotemporal evolutionary patterns remain largely unknown. Here, we compiled a data set including 3058 occurrence records assigned into 1203 species in China. We found that eriophyoid mite species richness is higher in the southeast and lower in the northwest of China, coinciding with the 500‐mm annual precipitation isoline. Using fragments of two mitochondrial genes (COI, 12S rRNA) and two nuclear genes (18S rRNA, 28S rRNA) of 304 eriophyoid mite species, we constructed a regional dated phylogenetic tree in China. Our spatiotemporal analysis revealed that mountains in eastern and southern China had high eriophyoid mite species richness and phylogenetic clustering, possessing both ancient and young mite lineages. Species distribution modelling (SDM) results showed that the predicted suitable area of eriophyoid mites slightly expanded from the last inter‐glacial period to the last glacial maximum period. In comparison with the current SDM, a significantly larger distribution range was observed in future scenarios. These findings suggest that mountains in eastern and southern China acted as not only cradles—recent rapid speciation, but also museums—centre of gradual accumulation and preservation and provide insights into monitoring and conserving eriophyoid mites.
Targeting mitochondrial Ca2+ uptake for the treatment of amyotrophic lateral sclerosis
Authors: Zhong Renjia , Rua Michael T. , Wei‐LaPierre Lan 
Published: November 2023

AbstractAmyotrophic lateral sclerosis (ALS) is a rare adult‐onset neurodegenerative disease characterized by progressive motor neuron (MN) loss, muscle denervation and paralysis. Over the past several decades, researchers have made tremendous efforts to understand the pathogenic mechanisms underpinning ALS, with much yet to be resolved. ALS is described as a non‐cell autonomous condition with pathology detected in both MNs and non‐neuronal cells, such as glial cells and skeletal muscle. Studies in ALS patient and animal models reveal ubiquitous abnormalities in mitochondrial structure and function, and disturbance of intracellular calcium homeostasis in various tissue types, suggesting a pivotal role of aberrant mitochondrial calcium uptake and dysfunctional calcium signalling cascades in ALS pathogenesis. Calcium signalling and mitochondrial dysfunction are intricately related to the manifestation of cell death contributing to MN loss and skeletal muscle dysfunction. In this review, we discuss the potential contribution of intracellular calcium signalling, particularly mitochondrial calcium uptake, in ALS pathogenesis. Functional consequences of excessive mitochondrial calcium uptake and possible therapeutic strategies targeting mitochondrial calcium uptake or the mitochondrial calcium uniporter, the main channel mediating mitochondrial calcium influx, are also discussed.
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Successful addition of topical antibiotic treatment after surgery in treatment-refractory nontuberculous mycobacterial skin and soft tissue infections
Authors: Stemkens Ralf , Cobussen Maarten , de Laat Erik , Hoefsloot Wouter , van Crevel Reinout , Aarnoutse Rob E. , van Ingen Jakko 
Published: November 2023

ABSTRACT
Treatment of skin and soft tissue infections with nontuberculous mycobacteria sometimes fails despite repeated debridements and long-term systemic antibiotic therapy. These treatment-refractory infections can cause significant morbidity and pose a treatment challenge. Following surgery, we treated three patients with negative pressure wound therapy with the instillation and dwell time of topical antibiotics, in addition to systemic antibiotic treatment. Treatment was successful and well tolerated, except for some local irritation.
Case Commentary: Another prong of attack? Topical antibiotic instillation with negative pressure wound therapy for nontuberculous mycobacterial skin and soft tissue infections
Authors: Nguyen David C. , Buettner Angel M. , Dousa Khalid M. 
Published: November 2023

ABSTRACT
Nontuberculous mycobacteria (NTM) skin infections remain therapeutically challenging. Given the diversity in infections, host responses, and antimicrobials, clinical guidelines are often built on case series and observational studies. In this commentary, we respond to a paper by Stemkens et al. that introduces an emerging strategy: adjunctive negative pressure wound therapy with instillation and dwell time combined with topical antibiotics for refractory NTM skin and soft tissue infections. We delve into the primary considerations surrounding this innovative approach.
Genome sequence and annotation of the B3 mycobacteriophage Phayeta
Authors: Bishop Emily , Earley Warren , Greco Alexandra , Hofseth Emma , Kinerson Emma , Lafayette Brandon , Llanot-Arocho Nestor , Mazen Brittney , Cevasco Megan , Williams Daniel C. 
Published: November 2023

ABSTRACT

Mycobacteriophage Phayeta was extracted from soil near Myrtle Beach, South Carolina using
Mycobacterium smegmatis
as a host. Annotation of the 68,700 base-pair circularly permuted genome identified 104 predicted protein-encoding genes, 34 of which have functional assignments.