bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enterocytozoon hepatopenaeiProliferate inProcambarus clarkii: A Warning for Crayfish and Shrimp Aquaculture
Authors: Ling Bingxin , Wu Yujiao , Yu Qing , Wang Chunxia , Hu Mengjiao , Meng Xianzhi , Long Mengxian , Pan Guoqing , Xiang Zhonghuai , Zhou Zeyang , Chen Jie 
Published: October 2023

AbstractThe pacific whiteleg shrimp (Litopenaeus vannamei) and the crayfish (Procambarus clarkii) are the most productive aquatic animals in the world. The prevalence of the microsporidiumEnterocytozoon hepatopenaei(EHP), an intracellular spore-forming unicellular parasite which leading retarded growth ofL. vannamei, has caused severe economic losses in most shrimp farming country. In this study, we found that the wildP. clarkii, living in shrimp ponds with EHP outbreak, excreted white faeces after temporary laboratory culture. The hepatopancreas (HP) of symptomatic crayfish exhibited a lighter color and severely atrophied. H&E-stain showed tissue lesions in both hepatopancreas and intestine, and clustered microsporidian spores were filled in the cytoplasm of the cells. PCR usingEHPptp2and two microsporidian-universal primers sets demonstrated the existence of EHP in the hepatopancreas, intestine and the white feces ofP. clarkii. The EHP loads of 103-104copies ofEHPptp2/50 ng HPgDNA were detected by qPCR. Developing stages and mature spores of EHP were observed in hepatopancreas ofP. clarkiithrough indirect-immunofluorescence assay (IFA) and transmission electron microscopy (TEM). Considering the large-scale cultivation ofL. vannameiandP. clarkii, overlap farming areas between these two species as well as the ability of crayfish to crawl on land and water, our finding indicates the potential role ofP. clarkiiin the transmission of EHP, and it is an early warning for crayfish and shrimp farming.
V-pipe 3.0: a sustainable pipeline for within-sample viral genetic diversity estimation
Authors: Fuhrmann Lara , Jablonski Kim Philipp , Topolsky Ivan , Batavia Aashil A , Borgsmüller Nico , Icer Baykal Pelin , Carrara Matteo , Chen Chaoran , Dondi Arthur , Dragan Monica , Dreifuss David , John Anika , Langer Benjamin , Okoniewski Michal , du Plessis Louis , Schmitt Uwe , Singer Franziska , Stadler Tanja , Beerenwinkel Niko 
Published: October 2023

AbstractThe large amount and diversity of viral genomic datasets generated by next-generation sequencing technologies poses a set of challenges for computational data analysis workflows, including rigorous quality control, adaptation to higher sample coverage, and tailored steps for specific applications. Here, we present V-pipe 3.0, a computational pipeline designed for analyzing next-generation sequencing data of short viral genomes. It is developed to enable reproducible, scalable, adaptable, and transparent inference of genetic diversity of viral samples. By presenting two large-scale data analysis projects, we demonstrate the effectiveness of V-pipe 3.0 in supporting sustainable viral genomic data science.
An inducible ESCRT-III inhibition tool to control HIV-1 budding
Authors: Wang Haiyan , Gallet Benoit , Moriscot Christine , Pezet Mylène , Chatellard Christine , Kleman Jean-Philippe , Göttlinger Heinrich , Weissenhorn Winfried , Boscheron Cécile 
Published: October 2023

AbstractHIV-1 budding as well as many other cellular processes require the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. Understanding the architecture of the native ESCRT-III complex at HIV-1 budding sites is limited due to spatial resolution and transient ESCRT-III recruitment. Here, we developed a drug-inducible transient HIV-1 budding inhibitory tool to enhance the ESCRT-III lifetime at budding sites. We generated auto-cleavable CHMP2A, CHMP3, and CHMP4B fusion proteins with the hepatitis C virus NS3 protease. We characterized the CHMP-NS3 fusion proteins in the absence and presence of protease inhibitor Glecaprevir with regard to expression, stability, localization and HIV-1 Gag VLP budding. Immunoblotting experiments revealed rapid and stable accumulation of CHMP-NS3 fusion proteins with variable modification of Gag VLP budding upon drug administration. Notably, CHMP2A-NS3 and CHMP4B-NS3 fusion proteins substantially decrease VLP release while CHMP3-NS3 exerted a minor effect and synergized with CHMP2A-NS3. Localization studies demonstrated the re-localization of CHMP-NS3 fusion proteins to the plasma membrane, endosomes, and Gag VLP budding sites. Through the combined use of transmission electron microscopy and video-microscopy, we unveiled drug-dependent accumulation of CHMP2A-NS3 and CHMP4B-NS3, causing a delay in HIV-1 Gag-VLP release. Our findings provide novel insight into the functional consequences of inhibiting ESCRT-III during HIV-1 budding and establish new tools to decipher the role of ESCRT-III at HIV-1 budding sites and other ESCRT-catalyzed cellular processes.
A Tetravalent Dengue Virus-like Particle Vaccine Induces High levels of Neutralizing Antibodies and Inhibits Dengue Replication in Non-Human Primates
Authors: Thoresen Daniel , Matsuda Kenta , Urakami Akane , Tun Mya Myat Ngwe , Nomura Takushi , Moi Meng Ling , Watanabe Yuri , Ishikawa Momoko , Hau Trang Thi Thu , Yamamoto Hiroyuki , Suzaki Yuriko , Ami Yasushi , Smith Jonathan F , Matano Tetsuro , Morita Kouichi , Akahata Wataru 
Published: October 2023

AbstractDengue virus (DENV) represents a significant global health burden, with 50% of the world’s population at risk of infection, and there is an urgent need for next-generation vaccines. Virus-like particle (VLP)-based vaccines, which mimic the antigenic structure of the authentic virus but lack the viral genome, are an attractive approach. Here we describe a dengue VLP (DENVLP) vaccine which generates a robust and long-lasting neutralizing antibody response against all four DENV serotypes in non-human primates. Importantly, DENVLP vaccination produced no ADE response against any of four DENV serotypes. Finally, we demonstrate in a non-human primate challenge model that DENVLP vaccination substantially reduces viral replication. We also transfer the purified IgG from the immunized monkeys into immunodeficient mice, where they protect against subsequent lethal dengue virus challenge, indicating a humoral mechanism of protection. These results indicate that a DENVLP vaccine is a safe and effective vaccine candidate.One Sentence Summary:Immunization of non-human primates with a tetravalent dengue VLP vaccine induces high levels of neutralizing antibodies and reduces the severity of infection for all four dengue serotypes.
Listeria monocytogenesutilizes glutathione and limited inorganic sulfur compounds as a source of essential L-cysteine
Authors: Berude John C. , Kennouche Paul , Reniere Michelle L. , Portnoy Daniel A. 
Published: October 2023

AbstractListeria monocytogenes(Lm) is a Gram-positive facultative intracellular pathogen that leads a biphasic lifecycle, transitioning its metabolism and selectively inducing virulence genes when it encounters mammalian hosts. Virulence gene expression is controlled by the master virulence regulator PrfA, which is allosterically activated by host- and bacterially-derived glutathione (GSH). The amino acid L-cysteine is the rate-limiting substrate for GSH synthesis in bacteria and is essential for bacterial growth. Unlike many bacteria,Lmis auxotrophic for L-cysteine and must import exogenous cysteine for growth and virulence. GSH is enriched in the host cytoplasm, and previous work suggests thatLmutilizes exogenous GSH for PrfA activation. Despite these observations, the import mechanism(s) for GSH remains elusive. Analysis of known GSH importers predicted a homologous importer inLmcomprised of the Ctp ABC transporter and the OppDF ATPases of the Opp oligopeptide importer. Here, we demonstrated that the Ctp complex is a high-affinity GSH/GSSG importer that is required forLmgrowth at physiologically relevant concentrations. Further, we demonstrated that OppDF are required for GSH/GSSG import in an Opp-independent manner. These data support a model where Ctp and OppDF form a unique complex for GSH/GSSG import that supports growth and pathogenesis. Additionally, we show thatLmutilizes the inorganic sulfur sources thiosulfate and H2S for growth in a CysK-dependent manner in the absence of other L-cysteine sources. These findings suggest a pathoadaptive role for partial cysteine auxotrophy inLm, where locally high GSH/GSSG or inorganic sulfur concentrations may signal arrival to distinct host niches.
Managing environmental sustainability in customer experience processes: A multi-stakeholder analysis within the food delivery ecosystem
Authors: Calza Francesco , Sorrentino Annarita , Tutore Ilaria 
Published: October 2023

PurposeThis paper aims to determine how environmental sustainability (ES) can be integrated into the customer experience (CX). In order to accomplish this, the paper uses a customer journey (CJ) perspective. Speculatively, the paper analyses the experiential stakeholder ecosystem beyond the CJ to verify the obstacles to the implementation of ES.Design/methodology/approachThis paper uses a qualitative analysis of in-depth interviews with multiple stakeholders within the food delivery ecosystem. The multi-stakeholder analysis allows the authors to explore the problem not only from an operational point of view but also from a strategic point of view since in the delivery of a service the value for the end customer is the result of the efforts of several players.FindingsThe results shed light on the importance attributed to ES by the players that make up the food delivery ecosystem. The findings emphasise the importance of an ecosystemic view amongst stakeholders to achieve ES.Originality/valueThis research extends the scarce and embryonic literature on a sustainable CX by applying a CJ perspective, by revealing how and with which touchpoints it is possible to be environmentally sustainable in the design of the CX.
Research on fatigue life prediction model for 2A70-T6 aluminum alloy at different strain ratios
Authors: Wei Yaobing , Li Yanan , Liu Jianhui , Wang Gai , Guo Yanlei , Pan Xuemei 
Published: October 2023

PurposeIn practical engineering, oil filters often work under asymmetric cyclic loading. In order to improve the prediction accuracy of fatigue life of the oil filters under asymmetric cyclic loading, the effect of strain ratio and low cycle fatigue plastic deformation on fatigue life need to be considered. This paper aims to discuss the aforementioned objective.Design/methodology/approachFirst, strain-controlled fatigue tests with strain ratios of 0, 0.5 and −1 were carried out on the oil filter material 2A70-T6 aluminum alloy, and the test data were used to obtain strain fatigue life curves at three strain ratios. Then, based on the idea of the constant life curve method, the average value of the ratio of the strain amplitude corresponding to different strain ratios under the same partial life was defined as the strain ratio factor. Finally, the elastic-plastic factor was modified by the strain ratio factor, and a new fatigue life prediction model considering the effect of strain ratio was proposed.FindingsThe proposed model was validated, respectively, by fatigue test data of 2A70-T6 aluminum alloy, 2124-T851 aluminum alloy and oil filter and the results of the proposed model were compared with the Coffin–Manson equation, Morrow model and Smith–Watson–Topper (SWT) model, showing that the proposed model had higher applicability and accuracy.Originality/valueIn this work, a strain ratio factor is established based on the idea of the constant life curve method, and the strain ratio factor is used to modify the introduced elastic-plastic factor, and then a new fatigue life prediction model considering the influence of strain ratio and low cycle fatigue plastic deformation on material fatigue damage accumulation is proposed. The results show that the prediction results of the proposed model are in good agreement with the experimental data, and the proposed model has good fatigue life prediction ability considering the influence of strain ratio and lays a foundation for the fatigue life prediction of the oil filter.
Does corruption grease or sand the wheels of firm productivity? Evidence of a non-linear relationship from a firm-level analysis in Vietnam
Authors: Ha Van Thi Cam , Nguyen Chau Trinh , Pham Tra Thi Thu , Nguyen Duy 
Published: October 2023

PurposeThis analysis examines the relationship between corruption and firm productivity in Vietnam.Design/methodology/approachThe authors apply the system generalized method of moments estimation approach on a panel dataset constructed from comprehensive enterprise surveys covering all the sectors over the 2011–2020 period.FindingsThe results confirm a non-linear relationship between corruption and firm productivity. Where corruption is severe, leaving corruption alone tends to benefit firm productivity because efforts to control corruption are likely to cause greater delays. In less corrupt provinces, corruption appears to harm firm productivity while efforts to control corruption provide significant productivity gains. This U-shaped relationship is confirmed for small firms and those in the private sector sub-samples. Intriguingly, this study reveals that the U-shaped relationship does not apply to micro, medium, large firms, state-owned firms and foreign-invested firms because corruption is found to have no significant impact on productivity among these sub-samples. Changes in regulations after 2014 toward promoting a transparent business environment are shown to foster the positive impact of lowering corruption on firm productivity.Research limitations/implicationsThis study suggests that lowering corruption is beneficial for firm productivity at the micro level. However, where corruption is severe, monitoring corruption alone is likely to cause adverse effects on productivity due to increased bureaucratic delays. Institutional reforms might play an important role in leveraging the effects of lowering corruption on productivity in highly corrupt areas.Originality/valueThis paper sheds new light on the relationship between corruption and firm productivity in the broad existing literature and especially in the limited number of studies for Vietnam.
Bibliometric investigation on green human resource management research
Authors: Mehta Poonam 
Published: October 2023

PurposeThe paper aims to present the comprehensive picture on green human resource management (GHRM) based upon bibliometric investigation, including different criteria, such as key authors, documents, keywords, bibliographic coupling (BC), etc.Design/methodology/approachBibliometric technique has been used with the application of VOSviewer. Further, the Scopus database has been utilised to extract the research articles.FindingsThe present study has exposed the due relevance of GHRM in the area of human resource management (HRM) for researchers as well as practitioners. The study has revealed that number of citations in the area is high from the researchers of China. Also, Jabbour, C.J.C., has been identified as the lead contributor and author from the area with 43 publications and 1,352 citations. The journal of cleaner production has been identified as the journal with the largest number of publications from the area. Also, prominent keywords used in the area are sustainability, GHRM, environmental management, sustainable environment and HRM.Research limitations/implicationsThe study has offered the comprehensive publication analysis on the area which will assist the researchers in gaining review on topic and development of empirical models. Further, with the utilisation of keywords criterion they can easily explore the publications done in the area. Also, study will help the scholars and researchers to identify the region and countries along with the institutions and universities to pursue research on the GHRM topic.Practical implicationsThe present study will help the mangers to focus on GHRM with more focus to acquire positive consequences for human resource management.Originality/valueThe present paper has presented various new and recent facets associated with the area of GHRM. Previously the similar analysis with bibliometric technique is rarely done with the present Scopus database.
Advantageous selection with intermediaries: a study of GSE‐securitized mortgage loans
Authors: Tsai Hsin‐Tien 
Published: October 2023

AbstractThis research studies the effects of mortgage subsidies and asymmetric information in the US mortgage market. I exploit discontinuities in interest rates generated by pricing rules and find patterns consistent with advantageous selection. I estimate an industry model that highlights the relationship between mortgage subsidies, intermediary lenders' incentives, and borrowers' advantageous selection. The model shows that mortgage subsidies enable advantageous selection, creating a deadweight loss of $7.90 billion. The counterfactual analysis reveals that pricing borrowers' private information eliminates advantageous selection only if mortgages are not subsidized. Without the mortgage subsidy, pricing borrowers' private information improves efficiency by $728.58 million.
One step at a time: Melanocytic differentiation in fusion‐driven cutaneous neoplasms
Authors: Linos Konstantinos 
Published: October 2023

AbstractAs dermatopathologists, we routinely diagnose melanocytic nevi, melanomas, and occasionally melanocytomas in our daily clinical practice. However, it is now clearly established that the presence of melanocytic differentiation in a tumor does not necessarily indicate any of the aforementioned diagnoses. Tumors such as clear cell sarcoma, malignant melanotic nerve sheath tumor, PEComa, melanotic neuroectodermic tumor of infancy, and even certain translocation‐associated renal cell carcinomas all share the common characteristic of melanin synthesis. Over the past two decades, with the advent of molecular diagnostics, there has been an explosion of new data and discoveries in this field. Examples such as CRTC1::TRIM11 cutaneous tumors and MITF pathway‐activated melanocytic tumors (ACTIN::MITF and MITF::CREM) have been incorporated into the latest edition of the WHO classification of skin tumors (5th ed). In a recent issue, Alexandrescu et al. reported another case of a dermal/subcutaneous melanocytic tumor harboring a MITF::CREM1 translocation. In a separate paper within the current issue, Li et al. present a case of clear cell sarcoma with the rare EWSR1::CREM fusion, which had initially been misdiagnosed as melanoma with regional and distant metastases. We warmly welcome these two very interesting and high‐quality articles to our journal, and we eagerly anticipate what the future holds for this fascinating category of tumors.
Wheat‐dependent exercise‐induced anaphylaxis: Subtypen, Diagnostik und Management
Authors: Faihs Valentina , Kugler Claudia , Schmalhofer Viktoria , Scherf Katharina Anne , Lexhaller Barbara , Mortz Charlotte G , Bindslev‐Jensen Carsten , Biedermann Tilo , Brockow Knut 
Published: October 2023

ZusammenfassungDie wheat‐dependent exercise‐induced anaphylaxis (Anstrengungs‐getriggerte Weizenallergie, WDEIA) ist eine IgE‐vermittelte Nahrungsmittelallergie, bei der es nur zu allergischen Symptomen kommt – welche von intermittierender Urtikaria bis hin zu schweren Anaphylaxien reichen können –, wenn Weizen im zeitlichen Zusammenhang mit verstärkenden Kofaktoren wie körperlicher Bewegung, nichtsteroidalen Antirheumatika oder Alkohol verzehrt wird. In den meisten Fällen weisen die Patienten eine Sensibilisierung auf ω5‐Gliadin in der Glutenfraktion des Weizens auf. ω5‐Gliadin‐negative Subtypen der WDEIA sind oft schwierig zu diagnostizieren und können durch Tri a 14 (Weizen‐Lipid‐Transferprotein), durch perkutane Sensibilisierung mit hydrolysierten Weizenproteinen oder, in seltenen Fällen, durch eine Gräserpollenkreuzreaktivität verursacht werden. Die Diagnose wird anhand der Anamnese in Kombination mit dem serologischen IgE‐Profil, Hauttests, Basophilenaktivierungstests und einer Provokationstestung mit Weizengluten und Kofaktoren gestellt. Die individuelle Ernährungsberatung ist nach wie vor die zentrale Säule im Management von Patienten mit WDEIA. Eine komplett weizenfreie Diät stellt eine mögliche Option dar, jedoch scheint diese die Toleranz weniger zu fördern als der weitergeführte regelmäßige Verzehr glutenhaltiger Getreidesorten, dieses allerdings nur bei gleichzeitiger Meidung von Kofaktoren. Alle Patienten sollten ein Notfallset zur Selbstbehandlung inklusive einem Adrenalin‐Autoinjektor erhalten und entsprechend geschult werden. Zur sublingualen Immuntherapie bei WDEIA, einer potenziell vielversprechenden therapeutischen Perspektive, werden weitere Daten benötigt. Dieser Artikel gibt einen Überblick über den aktuellen Wissensstand zur Diagnostik und zum Management bei WDEIA, einschließlich eines optimierten Provokationsprotokolls mit Weizengluten und Kofaktoren.
Die Prognose des Melanoms im Kopf‐Hals‐Bereich im Stadium II hängt vom histologischen Subtyp ab
Authors: Jasper Sophie , Keim Ulrike , Leiter Ulrike , Amaral Teresa , Flatz Lukas , Forschner Andrea 
Published: October 2023

ZusammenfassungHintergrund und ZieleDie Melanom‐Leitlinie basiert hauptsächlich auf dem AJCC‐Stadium. Hierbei wird nicht zwischen den histologischen Subtypen wie dem superfiziell spreitenden Melanom (SSM), dem Lentigo‐maligna‐Melanom (LMM) oder dem nodulären malignen Melanom (NM) unterschieden. Ziel der Studie war es zu untersuchen, ob sich Patienten mit LMM im klinischen Verlauf von Patienten mit SSM/NM unterscheiden. Dies ist aktuell besonders wichtig, da die adjuvante Anti‐PD‐1‐Therapie für Melanome im Stadium IIB und IIC zugelassen wurde.Patienten und MethodikDie Daten wurden aus dem Zentralregister „malignes Melanom“ entnommen. Es wurden nur Patienten mit LMM, SSM oder NM des Kopf‐Hals‐Bereichs und Primärdiagnose zwischen dem 01.01.2000 und dem 31.12.2019 eingeschlossen. Das progressionsfreie Überleben (PFÜ), das melanomspezifische Überleben (MSÜ) und das Metastasierungsmuster wurden für die Gruppe der LMM im Vergleich zur Gruppe der SSM/NM analysiert.ErgebnisseDie LMM‐Kohorte (n = 902) hatte ein signifikant besseres MSÜ als die SSM/NM‐Kohorte (n = 604). Beim PFÜ gab es keinen Unterschied. Das 5‐Jahres‐MSÜ der LMM‐Kohorte im Stadium II betrug 88,5 % (95 % KI 81,4–95,6), im Vergleich dazu das der SSM/NM‐Kohorte im Stadium II 79,7 % (95 % KI 72,8–86,6).SchlussfolgerungEs scheint nicht angebracht zu sein, eine adjuvante Therapie bei LMM‐Patienten im Stadium II im gleichen Umfang durchzuführen, wie bei Patienten mit SSM/NM.