bluuebunny/crossref_metadata_2025_split huggingface dataset:
PERCEPÇÕES SOBRE EMPREGABILIDADE E LIFELONG LEARNING ENTRE PESSOAS EMPREGADAS EM UMA CIDADE DO MEIO-OESTE CATARINENSE
Authors: Bragagnolo Sandra Mara , Alves da Luz Rafaela 
Published: October 2023

Lifelong learning é um termo inglês que, em tradução livre, significa “aprendizado ao longo da vida”. Trata-se de um conceito que preconiza a educação contínua, ou seja, sustenta a ideia de que os estudos devem ser permanentes, e não apenas durante um período da vida. O mercado de trabalho, volátil e competitivo, exige cada vez mais que o profissional esteja preparado para concretizar uma carreira de sucesso. A intenção deste trabalho é analisar as percepções sobre empregabilidade e lifelong learning entre pessoas empregadas na cidade de Fraiburgo-SC. A natureza da pesquisa é quantitativa, de tipologia descritiva. O método de coleta é um questionário e os dados coletados são analisados a partir de análise estatística com apoio de tabelas. Os resultados apontam que os respondestes atualmente estão fazendo curso de reciclagem profissional (59,4%) e procuram oportunidades de desenvolvimento quando não são oferecidas pela empresa (95,4%). Ainda, acreditam que ter um diploma em nível superior faz diferença positiva na hora de procurar uma boa colocação profissional (87,5%) e buscam aprender continuamente (93,7%). Também é possível verificar que 96,8% procuram realizar funções no trabalho que permitam aprender algo novo. Os resultados confirmam que as pessoas buscam atualização e novos conhecimentos para agregar em sua empregabilidade e gestão de carreira.
Palavras-Chave: Gestão de Carreira. Aprendizagem. Empregabilidade.

Abstract: Lifelong learning is an English term that, in free translation, means “lifelong learning”. It is a concept that advocates continuous education, that is, it supports the idea that studies should be permanent, and not just during a period of life. The job market, volatile and competitive, demands more and more that the professional is prepared to achieve a successful career. The intention of this work is to analyze the perceptions about employability and lifelong learning among people employed in the city of Fraiburgo-SC. The nature of the research is quantitative, with a descriptive typology. The collection method is a questionnaire and the collected data are analyzed from statistical analysis supported by tables. The results indicate that respondents are currently taking a professional recycling course (59.4%) and seek development opportunities when they are not offered by the company (95.4%). Still, they believe that having a higher education degree makes a positive difference when looking for a good job placement (87.5%) and seek continuous learning (93.7%). It is also possible to verify that 96.8% seek to carry out functions at work that allow them to learn something new. The results confirm that people seek updating and new knowledge to add to their employability and career management.
Keywords: Career Management. Continuous learning. Employability.
Callus Culture of <i>Aralia cordata</i> Thunb.: Obtaining, Selection of Cultivating Conditions, Somatic Embryogenesis Induction
Authors: Nekrasova D. A. , Povydysh M. N. , Pivovarova N. S. , Sidorov K. O. 
Published: October 2023

Introduction. Cell cultures spreads widely in different areas of biology, biotechnology and agriculture. Aralia cordata is a perennial herbaceous plant, which has been listed in Red book of the Russian Federation. Pharmaceuticals which are based on raw materials of Aralia ssp. have valuable types of pharmacological activity and are widely used in oriental medicine. A serious obstacle for resumption of the natural populations is the presence of the period of morphophysiological dormancy in the seeds of the plants, which requires a long-time stratification process. Limitation of the natural geographic range and the combination of biological activities useful for humans make Aralia cordata Thunb. prospective object for in vitro introduction.Aim. The aim of the study is obtaining of the viable cell culture of Aralia cordata Thunb., investigation of the somatic embryogenesis conditions.Materials and methods. Pieces of the leaves of Aralia cordata intact plant from Komarov Botanical Institute of the Russian Academy of Sciences were used as a primary explants. Pieces of leaves were sterilized in a 2 % benzalkonium chloride solution for 5 minutes, induction of primary callogenesis was carried out on Murasige – Skoog medium. Nutrient media with different constituents were discovered for choosing one for long-time cultivation of calli. Induction of somatic embryogenesis was carried out on the nutrient media with high auxins content. Ethanol extracts from the intact plant and calli cultures were assayed with HPTLC PRO SYSTEM (CAMAG AG, Switzerland).Results and discussion. After two weeks of cultivation, the formation of primary callus was observed on the surface of the explants. The Linsmaier – Skoog medium with a reduced amount of sucrose (20 g/l) was recognized as the most suitable medium for long-term maintenance of cultures. Embryoid structures of Aralia cordata have been obtained, now we are continuing to collect analytics data about this process. Qualitative analysis of the extracts showed that callus cultures accumulate triterpene glycosides, their composition is close to that of the intact plant.Conclusion. A viable strain of callus culture of Aralia cordata Thunb. was obtained, a nutrient medium for long-term cultivation of calli was established. Somatic embryoids have been obtained, and their further development is currently being monitored. A preliminary phytochemical study showed that the composition of the chemical components of calli is close to that of an intact plant.
Application of Thermodynamic Models to Predict the Solubility of Biologically Active Substances
Authors: Stepanov K. S. , Turmanidze G. N. , Sorokin V. V. , Sakharov A. D. 
Published: October 2023

Introduction. The choice of solvents for the processes of extraction of biologically active substances from natural raw materials, the processes of purification of natural and synthesized substances by extraction, crystallization and dissolution methods is an important problem of the modern pharmaceutical industry, because a large number of experiments must be performed to determine the optimal solvent or mixture of solvents. To reduce the cost of developing and optimizing the extraction and purification of substances stages, it is proposed to use thermodynamic models at the stage of preliminary solubility assessment. The article investigates predicting the solubility of pharmaceutical substances issue on the example of the technology for isolating hesperidin and glycyrrhizic acid from plant materials.Aim. Theoretical determination of the dissolving power of various solvents with respect to hesperidin and glycyrrhizic acid.Materials and methods. The PMUNIFAC and NRTL-SAC thermodynamic models were used to predict solubility. The solubility calculation for the NRTL-SAC model was performed using Aspen Properties V14 software, and for the PMUNIFAC model using PTC Mathcad Prime V6. To evaluate the results obtained using thermodynamic models, a number of experiments were carried out, the object of which was the peel of an orange (dried flavedo and albedo, the degree of grinding is 0.2–0.5 mm, the moisture content is 8 %). The quantitative content of hesperidin was determined by direct spectrophotometry at a wavelength of 290 nm. Statistical data processing was performed using Minitab v20 software (Minitab Inc., USA), differences were considered statistically significant at p < 0.05.Results and discussion. On the basis of thermodynamic models, the prediction of the solubility of hesperidin and glycyrrhizic acid was made. It has been shown that the solubility can be assessed both in the presence of solubility data according to the NRTL-SAC model, and in their complete absence according to PMUNIFAC. The correspondence of the theoretically calculated data to the experimental data confirms the correctness of the calculations of thermodynamic models. The results of the calculations are evaluated and solvents are recommended that can be used in the technology of isolating hesperidin and glycyrrhizic for the stages of degreasing, extraction and crystallization with the antisolvent.Conclusion. Using the NRTL-SAC and PMUNIFAC thermodynamic models, the dissolving power of various solvents with respect to hesperidin and glycyrrhizic acid was determined. From the calculation results, a list of solvents was built, ranked by the solubility of the studied substances in them. The resulting list can be used in the development of an industrial technology for the isolation and purification of hesperidin and glycyrrhizic acid. It is shown that the NRTL-SAC and PMUNIFAC models have good prospects for quantitative prediction of the solubility of active substances.
Kapasitas Total Antioksidan dan Uji Toksisitas Ekstrak Bunga Kecombrang (Etlingera elatior (Jack) R.M.Sm)
Authors: Hermawan Faiz Agung , Ferdinal Frans 
Published: October 2023

Oxidative stress is imbalance between antioxidant production and the formation of free radicals, which are involved in the development of degenerative diseases. Declining organ function is a symptom of degenerative diseases. The antioxidant helps protect the body from free radicals and prevent oxidative stress. Antioksidan classified into exogenous and endogenous. (Etlingera elatior (Jack) R.M.Sm) flower is known for its antioxidants. This study aimed to establish the toxicity and antioxidant features of kecombrang flower. Total antioxidant capacity test by Blois 1,1 Diphenyl-2Picryhydrazyl (DPPH) method, toxicity test by Brine Shrimp Lethality Test (BSLT) method. After acquiring simplicia by grinding and aerating the flowers, the extract was obtained by the maceration process using methanol as the solvent and evaporation. The extract was then analyzed. The total antioxidant capacity test showed an IC50 of 931.53 g/mL, indicating that kecombrang flower has a low total antioxidant capacity. Based on the results of the toxicology tests, the LC50 value for the kecombrang flower extract was 612.66 g/mL, indicating that it possessed toxic properties. This study concluded that kecombrang flower extract was shown to have low antioxidant levels and potential antimitotic activity.
PENGEMBANGAN LEMBAR KERJA PESERTA DIDIK BERBASIS NUMBERED HEADS TOGETHER PADA MATERI ZAT ADITIF KELAS VIII
Authors: Deswita Aji Astutik  , Oktaffi Arinna Manasikana  
Published: October 2023

Penelitian ini bermaksud untuk membuat dan mengukur valid, praktis dan efektif LKPD berbasis numbered heads together pada materi zat aditif kelas VIII. Metode penelitian mengintisarikan pengembangan model ADDIE yang dilakukan lima tahap ialah analyze, design, development, implementation, dan evaluation. Hasil dari validasi LKPD oleh dua validator ahli dan pendidik didapatkan skor rerata sebesar 77,8% dengan kategori valid. Hasil kepraktisan berdasarkan keterlaksanaan penggunaan LKPD didapatkan skor rata-rata 88,6% dan hasil respon positif peserta didik didapatkan skor rerata sebesar 92.8% dengan kelompok sangat praktis. Hasil keefektifan mengolah ketuntasan hasil belajar peserta didik didapatkan skor rerata78,7% dengan kategori efektif.
Kata Kunci : Pengembangan LKPD, Model Numbered Heads Together, Zat Aditif.
The antivirulence activity, transcriptomics of EGCG and its protective effects on zebrafish infected by Aeromonas hydrophila
Authors: Yin Hongmei , Yan Qiaohua , Cheng Guoqiang , Zhang Li , Li Meiqing , Hu Tingting , Gao Sihui , Chen Yuanhang , Tang Huaqiao , Luo Jie 
Published: October 2023

BackgroundAeromonas hydrophila is an important pathogen that mainly harms aquatic animals and exhibits resistance to a variety of antibiotics. This study investigated the effect of epigallocatechin-3-gallate (EGCG) on the virulence factors of A.hydrophila and its impact on adhesion, invasion, and cytotoxicity in Caco-2 cells. The potential mechanism of antibacterial activity of EGCG was investigated by transcriptomic analysis.ResultsEGCG not only inhibited the production of biofilm, hemolytic activity, motility, and protease activity of A.hydrophila, but also reduced its adhesion, invasion, and cytotoxicity in Caco-2 cells. Transcriptomic analysis indicated that the antimicrobial activity of EGCG may be achieved by weakening the chemotaxis and stress response of the bacteria, as well as inhibiting the TonB system. Animal studies demonstrated that EGCG can significantly improve the survival rate and organs damage of zebrafish infected with A.hydrophila.ConclusionEGCG would be a potential alternative drug for the prevention and treatment of A. hydrophila infections by anti-virulence mechanism.
Assessment of the activities physiological cost of the defense forces officers in Ukraine using miniature ECG device
Authors: Bocharov Mykhailo , Stasiuk Vasyl , Osyodlo Vasyl , Ryzhenko Tetyana , Malanin Vlad , Chumachenko Dmytro , Chaikovsky Illya 
Published: October 2023

IntroductionIn the principles of the organization of armed struggle of the defense forces of most developed countries of the world, considerable attention is paid to the evaluation of combat readiness of the military personnel. This procedure is conditioned by such interconnected goals of the armed struggle as the maximum realization of the combat potential and the minimization of personnel losses. The purpose of the work is to determine the physiological cost of the activities of the soldiers of the Defense Forces of Ukraine with the help of miniature electrocardiographic hardware and software complexes.MethodsIn the research, ultra-miniature ECG devices worn on the body for a long time, so-called wearable “on-body” ECG patch devices, were used in various combat conditions. When analyzing the data, the principle of multi-faceted ECG analysis was implemented, which allows you to obtain complete and physiologically based information, which includes 4 blocks: heart rate variability (HRV), amplitude-time indicators of the ECG, heart rhythm disorders, and psycho- emotional state.ResultsIn this study, a complex index of the functional state formed based on estimates of generally accepted and original indicators of heart rhythm variability, the shape of the teeth and complexes of the electrocardiogram, as well as an index of the psycho-emotional state formed according to the same principles based on the analysis of heart rhythm variability according to the modified McCraty algorithm (USA) was evaluated. Examination with the help of the complex is carried out in a state of rest, sitting or lying down.DiscussionThe sensitivity of the developed monitoring system is good enough to detect the changes in the functional state both in the case of short-term (for hours) intense physical or psycho-emotional stress and more chronic (for days and weeks) stress depending on the nature of the task being done. The proposed methods and means can be considered an important tool to support the commander's decision-making regarding the ability of personnel from the point of view of their functional state to perform combat tasks.
A cross-sectional survey on mother-to-child transmission of HIV among the migrant population in Dongguan, China
Authors: Pan Congcong , Pan Haiyan , Liang Dongmei , Liu Yuanyuan , Yin Sichun , Zhong Jianbo , He Songmei 
Published: October 2023

IntroductionThe migrant population, consisting of individuals who relocate from rural to urban areas, faces unique challenges that heighten their vulnerability to HIV infection. These challenges stem from a combination of sociodemographic factors and limited access to healthcare services. Understanding the dynamics of HIV transmission within this population is crucial for the development of effective prevention strategies.MethodsTo investigate the factors contributing to HIV vulnerability among migrants, we conducted a cross-sectional study at Dongguan People's Hospital from January 1, 2018, to December 31, 2021. Our study focused on pregnant women living with HIV and their infants, with a particular emphasis on sociodemographic characteristics, HIV testing and treatment profiles, and neonatal clinical data. Data were systematically collected using standardized forms.ResultsAnalysis of data from 98 participants revealed noteworthy findings. No significant associations were observed between age, marital status, and educational background regarding HIV vulnerability. Similarly, factors such as the status of sexual partners, spousal therapy, and the number of children had no significant impact. However, our analysis highlighted the critical role of treatment strategies for HIV-positive women and the timing of antiretroviral therapy initiation for women with HIV, both of which were associated with HIV transmission (P &lt; 0.05). Additionally, factors such as feeding type, neonatal antiretroviral prophylaxis, and preventive treatment strategies showed significant associations, while the preventive treatment program for neonates demonstrated no significant impact.DiscussionThese findings provide valuable insights into the specific risk factors and barriers to HIV prevention faced by the migrant population in Dongguan. They underscore the importance of targeted interventions and policies aimed at curtailing mother-to-child HIV transmission. By addressing the unique challenges experienced by migrant mothers and their infants, this study contributes significantly to broader efforts in controlling the spread of HIV, ultimately enhancing the health outcomes and well-being of Dongguan's migrant population. Furthermore, our research introduces a distinctive perspective within the extensively examined domain of Prevention of Mother-to-Child Transmission (PMTCT) programs, focusing on the internally migrant Chinese population, an understudied demographic group in this context. This study, conducted in Dongguan, China, represents one of the pioneering investigations into pregnant women with HIV and their infants within this migrant community.
Causal association between adiposity and hemorrhoids: a Mendelian randomization study
Authors: Huang Jian , Gui Ying , Qin Hongping , Xie Yubo 
Published: October 2023

BackgroundHemorrhoids are a very common anorectal disorder affecting a large number of individuals throughout the world. This study aimed to evaluate the causal effects of four adiposity traits including body mass index (BMI), body fat percentage, waist circumference, and waist-to-hip ratio on hemorrhoids by Mendelian randomization (MR).MethodsWe used summary statistics of BMI (N = 461,460), body fat percentage (N = 454,633), waist circumference (N = 462,166), waist-to-hip ratio (N = 212,244), and hemorrhoids (N = 337,199) from large-scale genome wide association studies of European ancestry. Univariable and multivariable MR were carried out to infer causality. The MR Steiger directionality test was used to test the causal direction.ResultsThe primary MR analysis using the inverse variance weighted (IVW) method showed that there were positive effects of genetically determined BMI [odds ratio (OR) = 1.005, 95% confidence interval (CI): 1.003–1.008, per standard deviation (SD), p = 7.801 × 10−5], body fat percentage (OR = 1.005, 95% CI: 1.001–1.008, per SD, p = 0.008), waist circumference (OR = 1.008, 95% CI: 1.005–1.011, per SD, p = 1.051 × 10−6), and waist-to-hip ratio (OR = 1.010, 95% CI: 1.003–1.017, per SD, p = 0.003) on hemorrhoids. These findings were robust in multivariable MR adjusting for physical activity. The Steiger directionality test showed evidence against reverse causation.ConclusionOur MR study supports a causal role of adiposity in the development of hemorrhoids. Adiposity prevention may be an important strategy for reducing hemorrhoids risk.
Effect of epidural block on surgical conditions during pediatric subumbilical laparoscopic surgery involving a supraglottic airway: a randomized clinical trial
Authors: Wu Lei , Wei Siwei , Xiang Zhen , Yu Eryou , Chen Zheng , Du Zhen , Qu Shuang Quan 
Published: October 2023

BackgroundFew studies have examined the effect of epidural block on surgical conditions during pediatric subumbilical laparoscopic surgery involving a supraglottic airway (SGA). This study investigated the surgical condition scores for such procedures in cases where neuromuscular block, epidural block, or neither was used.MethodsA total of 150 patients aged 3–12 years undergoing laparoscopic orchiopexy with a ProSeal SGA device were randomly allocated to one of three groups: the control group (did not receive neuromuscular block and epidural block), the NMB group [received a neuromuscular block (train-of-four 1–2 twitches) using rocuronium], or the EDB group (received an epidural block using ropivacaine). The primary outcome was the quality of surgical conditions evaluated with the Leiden-Surgical Rating Scale by the blinded surgeon. The secondary outcome measures included intraoperative hemodynamic data (including mean arterial pressure and heart rate), the SGA device removal time, the PACU discharge time, the pain score in the PACU and intraoperative adverse events (including bradycardia, hypotension, peak airway pressure &gt; 20 cmH2O, and poor or extremely poor surgical conditions occurred during the operation). Statistical analysis was performed with one-way analysis of variance, the Kruskal–Wallis test, the chi-square test or Fisher’s exact test. Bonferroni corrections for multiple comparisons were made for primary and secondary outcomes.ResultsSurgical condition scores were significantly higher in the NMB and EDB groups than in the control group (median difference: 0.8; 95% confidence interval [CI], 0.5–1.0; p &lt; 0.0001; and median difference: 0.7; 95% CI, 0.5–0.8; p &lt; 0.0001, respectively). Blood pressure and heart rate were significantly lower in the EDB group than in the other two groups (p &lt; 0.0001 and p = 0.004). Patients in the EDB group had significantly lower pain scores during PACU than those in the other two groups (p &lt; 0.0001). The sufentanil dose was lower in the EDB group than in the other two groups (p = 0.001).ConclusionEpidural block can improve surgical conditions during pediatric subumbilical laparoscopic surgery involving a SGA to a degree comparable to that with moderate neuromuscular block.