bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural organization and interactions of transmembrane domains in tetraspanin proteins
Authors: Kovalenko Oleg V , Metcalf Douglas G , DeGrado William F , Hemler Martin E 
Published: June 2005

Abstract

Background
Proteins of the tetraspanin family contain four transmembrane domains (TM1-4) linked by two extracellular loops and a short intracellular loop, and have short intracellular N- and C-termini. While structure and function analysis of the larger extracellular loop has been performed, the organization and role of transmembrane domains have not been systematically assessed.

Results
Among 28 human tetraspanin proteins, the TM1-3 sequences display a distinct heptad repeat motif (
abcdefg
)n. In TM1, position
a
is occupied by structurally conserved bulky residues and position
d
contains highly conserved Asn and Gly residues. In TM2, position
a
is occupied by conserved small residues (Gly/Ala/Thr), and position
d
has a conserved Gly and two bulky aliphatic residues. In TM3, three
a
positions of the heptad repeat are filled by two leucines and a glutamate/glutamine residue, and two
d
positions are occupied by either Phe/Tyr or Val/Ile/Leu residues. No heptad motif is apparent in TM4 sequences. Mutations of conserved glycines in human CD9 (Gly25 and Gly32 in TM1; Gly67 and Gly74 in TM2) caused aggregation of mutant proteins inside the cell. Modeling of the TM1-TM2 interface in CD9, using a novel algorithm, predicts tight packing of conserved bulky residues against conserved Gly residues along the two helices. The homodimeric interface of CD9 was mapped, by disulfide cross-linking of single-cysteine mutants, to the vicinity of residues Leu14 and Phe17 in TM1 (positions
g
and
c
) and Gly77, Gly80 and Ala81 in TM2 (positions
d
,
g
and
a
, respectively). Mutations of
a
and
d
residues in both TM1 and TM2 (Gly25, Gly32, Gly67 and Gly74), involved in intra molecular TM1-TM2 interaction, also strongly diminished inter molecular interaction, as assessed by cross-linking of Cys80.

Conclusion
Our results suggest that tetraspanin intra- and intermolecular interactions are mediated by conserved residues in adjacent, but distinct regions of TM1 and TM2. A key structural element that defines TM1-TM2 interaction in tetraspanins is the specific packing of bulky residues against small residues.
PMD2HD – A web tool aligning a PubMed search results page with the local German Cancer Research Centre library collection
Authors: Bohne-Lang Andreas , Lang Elke , Taube Anke 
Published: June 2005

Abstract

Background
Web-based searching is the accepted contemporary mode of retrieving relevant literature, and retrieving as many full text articles as possible is a typical prerequisite for research success. In most cases only a proportion of references will be directly accessible as digital reprints through displayed links. A large number of references, however, have to be verified in library catalogues and, depending on their availability, are accessible as print holdings or by interlibrary loan request.

Methods
The problem of verifying local print holdings from an initial retrieval set of citations can be solved using Z39.50, an ANSI protocol for interactively querying library information systems. Numerous systems include Z39.50 interfaces and therefore can process Z39.50 interactive requests. However, the programmed query interaction command structure is non-intuitive and inaccessible to the average biomedical researcher. For the typical user, it is necessary to implement the protocol within a tool that hides and handles Z39.50 syntax, presenting a comfortable user interface.

Results
PMD2HD is a web tool implementing Z39.50 to provide an appropriately functional and usable interface to integrate into the typical workflow that follows an initial PubMed literature search, providing users with an immediate asset to assist in the most tedious step in literature retrieval, checking for subscription holdings against a local online catalogue.

Conclusion
PMD2HD can facilitate literature access considerably with respect to the time and cost of manual comparisons of search results with local catalogue holdings. The example presented in this article is related to the library system and collections of the German Cancer Research Centre. However, the PMD2HD software architecture and use of common Z39.50 protocol commands allow for transfer to a broad range of scientific libraries using Z39.50-compatible library information systems.
Improved Survival of Follicular Lymphoma Patients in the United States
Authors: Swenson Wade T. , Wooldridge James E. , Lynch Charles F. , Forman-Hoffman Valerie L. , Chrischilles Elizabeth , Link Brian K. 
Published: June 2005

Purpose Despite several new treatment options, single- and multi-institution analyses have not clarified whether survival patterns in follicular lymphoma (FL) patients have changed in recent decades. We undertook a study using a large population-based registry to analyze survival patterns among patients with FL. Patients and Methods Surveillance, Epidemiology, and End Results morphology codes were used to identify 14,564 patients diagnosed with FL between 1978 and 1999. Observed median survival times, Kaplan-Meier survival curves, proportional death hazard ratios, and relative survival rates were calculated. Joinpoint regression analysis was used to identify trends in annual adjusted death hazard ratios. Results An improvement in survival of all patients with FL was observed between each of three diagnosis eras (1978 to 1985, 1986 to 1992, and 1993 to 1999) by log-rank tests. Among patients with stage-specific data, the median survival time improved from 84 months (95% CI, 81 to 88 months) in the 1983 to 1989 era to 93 months (95% CI, 89 to 97 months) in the 1993 to 1999 era. Similar findings were identified across sex and age groups and for subsets including advanced-stage, large-cell FL and the combined subset of small cleaved- and mixed-cell FL. The inter-era survival advantage observed in white patients was not observed for black patients. The relative risk of death decreased by 1.8% per year over the 1983 to 1999 observation period. Conclusion The survival of patients with FL in the United States has improved over the last 25 years. The survival improvement may be a result of the sequential application of effective therapies and improved supportive care.
Predictive Value of Bone Resorption and Formation Markers in Cancer Patients With Bone Metastases Receiving the Bisphosphonate Zoledronic Acid
Authors: Coleman Robert E. , Major Pierre , Lipton Allan , Brown Janet E. , Lee Ker-Ai , Smith Matthew , Saad Fred , Zheng Ming , Hei Yong Jiang , Seaman John , Cook Richard 
Published: June 2005

Purpose Three large, randomized trials of patients with bone metastases recently demonstrated that zoledronic acid reduces the risk of skeletal-related events. These trials provide an opportunity for investigating the correlation between bone metabolism and clinical outcome during bisphosphonate therapy. Patients and Methods Urinary measurements of N-telopeptide (Ntx) and serum bone alkaline phosphatase (BAP) were obtained in 1,824 bisphosphonate-treated patients—1,462 with zoledronic acid (breast, 490; prostate, 411; myeloma, 210; non–small-cell lung, 183; other, 168) and 362 with pamidronate (breast, 254; myeloma, 108). This exploratory cohort analysis grouped patients by baseline and most recent levels of Ntx as low (< 50 nmol/mmol creatinine), moderate (50 to 99 nmol/mmol creatinine), or high (≥ 100 nmol/mmol creatinine), and BAP as low (< 146 U/L) or high (≥ 146 U/L). The relative risks for negative clinical outcomes were estimated for each group using multiple-event and Cox regression models with time-varying covariates. Results Patients with high and moderate Ntx levels had 2-fold increases in their risk of skeletal complications and disease progression compared with patients with low Ntx levels (P < .001 for all). High Ntx levels in each solid tumor category were associated with a 4- to 6-fold increased risk of death on study, and moderate Ntx levels a 2- to 4-fold increased risk compared with low Ntx levels (P < .001 for all). Bone alkaline phosphatase also showed some correlation with risk of negative clinical outcomes. Conclusion The bone resorption marker Ntx provides valuable prognostic information in patients with bone metastases receiving bisphosphonates.
Effects of Exemestane Administered for 2 Years Versus Placebo on Bone Mineral Density, Bone Biomarkers, and Plasma Lipids in Patients With Surgically Resected Early Breast Cancer
Authors: Lønning Per E. , Geisler Jürgen , Krag Lars E. , Erikstein Bjørn , Bremnes Yngve , Hagen Anne I. , Schlichting Ellen , Lien Ernst A. , Øfjord Erik S. , Paolini Jolanda , Polli Anna , Massimini Giorgio 
Published: June 2005

Purpose To evaluate potential detrimental effects of exemestane on bone and lipid metabolism. Patients and Methods Postmenopausal women with early breast cancer were randomly assigned to exemestane 25 mg daily or placebo for 2 years in a double-blind setting. Primary objective was to evaluate the effect of exemestane on bone mineral density. Secondary objectives were effects on bone biomarkers, plasma lipids, coagulation factors, and homocysteine. Planned size was 128 patients. Results One hundred forty-seven patients were enrolled. All patients completed their 24-month visit except for those discontinuing treatment at an earlier stage. The mean annual rate of bone mineral density loss was 2.17% v 1.84% in the lumbar spine (P = .568) and 2.72% v 1.48% in the femoral neck (P = .024) in the exemestane and placebo arm, respectively. The mean change in T-score after 2 years was −0.21 for exemestane and −0.11 on placebo in the hip, and −0.30 and −0.21, respectively, in the lumbar spine. Exemestane significantly increased serum level and urinary excretion of bone resorption, but also bone formation markers. Except for a modest reduction in high-density lipoprotein cholesterol (P < .001) and apolipoprotein A1 (P = .004), exemestane had no major effect on lipid profile, homocysteine levels, or coagulation parameters. Conclusion Exemestane modestly enhanced bone loss from the femoral neck without significant influence on lumbar bone loss. Except for a 6% to 9% drop in plasma high-density lipoprotein cholesterol, no major effects on serum lipids, coagulation factors, or homocysteine were recorded. Bone mineral density should be assessed according to the US Preventive Services Task Force guidelines.
Phase II Trial of Single-Agent Temsirolimus (CCI-779) for Relapsed Mantle Cell Lymphoma
Authors: Witzig Thomas E. , Geyer Susan M. , Ghobrial Irene , Inwards David J. , Fonseca Rafael , Kurtin Paul , Ansell Stephen M. , Luyun Ronnie , Flynn Patrick J. , Morton Roscoe F. , Dakhil Shaker R. , Gross Howard , Kaufmann Scott H. 
Published: June 2005

PurposeMantle cell lymphoma (MCL) is characterized by a t(11;14) resulting in overexpression of cyclin D1 messenger RNA. This study tested whether temsirolimus (previously known as CCI-779), an inhibitor of the mammalian target of rapamycin kinase that regulates cyclin D1 translation, could produce tumor responses in patients with MCL.Patients and MethodsPatients with relapsed or refractory MCL were eligible to receive temsirolimus 250 mg intravenously every week as a single agent. Patients with a tumor response after six cycles were eligible to continue drug for a total of 12 cycles or two cycles after complete remission, and were then observed without maintenance.ResultsThirty-five patients were enrolled and were assessable for toxicity; one patient had MCL by histology but was cyclin D1 negative and was ineligible for efficacy. The median age was 70 years (range, 38 to 89 years), 91% were stage 4, and 69% had two or more extranodal sites. Patients had received a median of three prior therapies (range, one to 11), and 54% were refractory to the last treatment. The overall response rate was 38% (13 of 34 patients; 90% CI, 24% to 54%) with one complete response (3%) and 12 partial responses (35%). The median time-to-progression in all patients was 6.5 months (95% CI, 2.9 to 8.3 months), and the duration of response for the 13 responders was 6.9 months (95% CI, 5.2 to 12.4 months). Hematologic toxicities were the most common, with 71% (25 of 35 patients) having grade 3 and 11% (four of 35 patients) having grade 4 toxicities observed. Thrombocytopenia was the most frequent cause of dose reductions but was of short duration, typically resolving within 1 week.ConclusionsSingle-agent temsirolimus has substantial antitumor activity in relapsed MCL. This study demonstrates that agents that selectively target cellular pathways dysregulated in MCL cells can produce therapeutic benefit. Further studies of this agent in MCL and other lymphoid malignancies are warranted.
Outcome of cognitive behaviour therapy for minor depression in routine practice
Authors: Cuijpers Pim , Smit Filip , Voordouw Ineke , Kramer Jeannet 
Published: June 2005

Objectives. To examine (1) whether the improvement in depressive symptomatology in subjects participating in psychoeducational groups for minor depression in routine practice is comparable to the improvement realized in a randomized efficacy trial; and (2) whether the level of depressive symptoms of subjects who participated in this intervention is similar after treatment to the level of depressive symptoms of the general population.Design. Participants (N=187) of 20 psychoeducational groups in routine practice in the Netherlands were examined before and after the intervention using the Centre for Epidemiological Studies – Depression scale (CES‐D).Methods. The standardized improvement from pre‐ to post‐test in subjects was compared to the improvement found in subjects participating in a randomized trial of the same intervention. Furthermore, we compared the post‐test scores to the scores of the general population.Results. The improvement of depressive symptoms in routine practice was of the same magnitude as the improvement in the randomized trial. However, a considerable proportion of the participants (54.5%) still scored above the cut‐off score of the CES‐D at post‐test, and the mean CES‐D score of the participants (M=17.0; SD=9.8) differed significantly (p<.01) from the mean score in the general population (M=9.7; SD=8.6).Conclusion. Psychoeducational intervention can be an important help for people with depressive symptoms. The improvement in terms of depressive symptoms in routine practice does not differ from the improvement found in a randomized trial. However, participants remained considerably more depressed than the general population and this intervention is, for many, not sufficient as a form of treatment.
Who benefits from inpatient short‐term psychotherapy in the long run? Patients' evaluations, outpatient after‐care and determinants of outcome
Authors: Beutel M. E. , Höflich A. , Kurth R. A. , Reimer Ch. 
Published: June 2005

Background. Little work has been published on short‐term inpatient treatments combining the virtues of inpatient treatments and short‐term psychotherapy. The purposes of this study are to (a) determine the outcomes of short‐term psychodynamic inpatient psychotherapy from patients' and therapists' perspectives, (b) assess the stability of changes, and (c) identify predictors of long‐term outcome.Methods. 83 consecutive inpatients (76% of those eligible) were assessed at intake, discharge, and 1 year follow‐up by standardized questionnaires regarding symptoms (SCL‐90R), interpersonal problems (IIP), object relationships (IPO), and the therapeutic relationship (HAQ). Diagnoses and functioning (GAF) were assessed by the therapists.Results. Psychological distress (effect size S=1.14) and physical complaints (ES=1.03) decreased strongly from intake to discharge, the majority of patients moving from the pathological to the normal range of the global severity index GSI (SCL‐90R). At follow‐up, average distress remained at a low level, and the majority of patients, and their therapists and physicians, regarded their well‐being as improved. In a multivariate approach, a substantial proportion of variance of the distress at follow‐up (42%) was explained by an infantile object relationship pattern (IPO), social avoidance (IIP), negative vocational changes, and a lack of a confidant at follow‐up. A delayed start of subsequent ambulatory psychotherapy was another negative outcome predictor.Conclusions. Attention should be given to maladaptive interpersonal relationship patterns, to vocational reintegration, and a confiding relationship as potential predictors of long‐term outcome. Also, patterns of ambulatory after‐care following inpatient treatment need further scrutiny.
Clinician reports of personality pathology of patients beginning and patients ending psychoanalysis
Authors: Cogan Rosemary , Porcerelli John H. 
Published: June 2005

Objectives. The purpose of this work was to use a clinician Q‐sort procedure to describe the personality pathology and adaptive functioning of patients beginning and ending psychoanalysis.Design. With a cross‐sectional design, we compared a group of patients beginning and a group of patients ending psychoanalysis.Methods. Twenty‐six psychoanalysts described a patient beginning psychoanalysis and 26 described a patient ending psychoanalysis using the Shedler‐Westen Assessment Procedure 200 (SWAP‐200). Each clinician also completed questions about themselves, the patient, and the treatment. The most characteristic SWAP‐200 items describing patients beginning and patients ending psychoanalysis provide a meaningful picture of the two groups.Results. Among patients at the end of psychoanalysis, scores were significantly lower on the SWAP‐200 Paranoid, Schizotypal, Borderline, Histrionic, and Dependent scales and scores were significantly higher on the SWAP‐200 High functioning scale and the DSM‐IV GAF scale. Common characteristics of patients beginning psychoanalysis were anxiety, guilt, and shame. Common characteristics of patients ending psychoanalysis were conscientiousness and responsibility, striving to live up to moral and ethical standards, and enjoyment of challenges.Conclusions. The findings demonstrate the usefulness of a clinician report measure for the study of psychoanalytic psychotherapy and psychoanalysis.
Review of Radio Studies teaching
Authors: Price-Davies Eryl , Wall Tim , Windows Peter , McClellan Tim , Dubber Andrew , Keith Michael C. , Berryman Bruce , Duffield Lee 
Published: June 2005

This survey of current approaches to the teaching of radio studies in universities seeks to identify common themes and elements of such courses. In addition, it identifies notable examples of interesting, unusual and creative pedagogic approaches, based on contributions volunteered
by university teachers of radio in Britain, Australia, New Zealand and the USA. The introduction by Eryl Price-Davies covers the variety of approaches to radio in UK degree programmes, which include theory and practice, critical listening skills, history and archives, vocations in the radio
industries, and the actual practice of broadcasting within a course. The contributions that follow this illustrate international examples of some of these approaches. These include the pre-produced radio interview programme as a lecture, ‘visualisation’ lectures, web-radio production
training, individual students broadcasting from an overseas/exchange assignment, and programmes with a developed radio route - comprising several modules or units.
1.
Introduction: some thematic observations on the teaching of radio studies
(Eryl Price-Davies)
2.
BA
(Hons.) Media and Communications (Radio): a case study. The specialist radio degree at UCE, Birmingham, UK
(Tim Wall and Peter Windows)
3.
Touching the imagination through sound: using visualization to develop undergraduate creativity
(Tim McClellan)
4.
The
radio interview as teaching: how listening to a radio show can beat the in-person lecture
(Andrew Dubber)
5.
A debating point: radio in the curriculum in the United States of America
(Michael C. Keith)
6.
From on-air to the Web: Re-defining the radio producer

(Bruce Berryman)
7.
QUT's international radio reporting field trips
(Lee Duffield)
Vom Stau zur Verkehrsinformation – Datenfusion als Teil eines Gesamtprozesses (From Jams on the Road to Traffic Information – Data Fusion as a Part of the Traffic Modelling Process)
Authors: Vortisch Peter , Ortgiese Michael 
Published: June 2005

Abstract
Optimal informiert zu sein – der Wunsch wohl der meisten Verkehrsteilnehmer. Eine Vielzahl von Aktivitäten versuchen, diesem Wunsch immer stärker zu entsprechen. Im Zentrum dieser Bemühungen steht die Fusion möglichst vieler Eingangsdaten zu einer Verkehrslage. Diese komplexen technischen und wirtschaftlichen Inhalte sollen mit Blick auf die folgenden Fragestellungen beleuchtet werden: Was verbirgt sich hinter dem Begriff Verkehrlage? Welche Datenquellen können zu einer Verkehrslage fusioniert werden? Welche technischen Ansätze können zur Fusion genutzt werden? Wie sollten die unterschiedlichen Akteure im Gesamtprozess zusammenarbeiten? Was sind die Rahmenbedingungen, die im Kontext der Wertschöpfungskette bei der Datenfusion zu berücksichtigen sind?
STERILIZED in the Name of Public Health
Authors: Stern Alexandra Minna 
Published: June 2005

In exploring the history of involuntary sterilization in California, I connect the approximately 20 000 operations performed on patients in state institutions between 1909 and 1979 to the federally funded procedures carried out at a Los Angeles County hospital in the early 1970s. Highlighting the confluence of factors that facilitated widespread sterilization abuse in the early 1970s, I trace prosterilization arguments predicated on the protection of public health. This historical overview raises important questions about the legacy of eugenics in contemporary California and relates the past to recent developments in health care delivery and genetic screening.
Injuries at Work in the US Adult Population: Contributions to the Total Injury Burden
Authors: Smith Gordon S. , Wellman Helen M. , Sorock Gary S. , Warner Margaret , Courtney Theodore K. , Pransky Glenn S. , Fingerhut Lois A. 
Published: June 2005

Objectives. We estimated the contribution of nonfatal work-related injuries on the injury burden among working-age adults (aged 18–64 years) in the United States. Methods. We used the 1997–1999 National Health Interview Survey (NHIS) to estimate injury rates and proportions of work-related vs non–work-related injuries. Results. An estimated 19.4 million medically treated injuries occurred annually to working-age adults (11.7 episodes per 100 persons; 95% confidence interval [CI]=11.3, 12.1); 29%, or 5.5 million (4.5 per 100 persons; 95% CI=4.2, 4.7), occurred at work and varied by gender, age, and race/ethnicity. Among employed persons, 38% of injuries occurred at work, and among employed men aged 55–64 years, 49% of injuries occurred at work. Conclusions. Injuries at work comprise a substantial part of the injury burden, accounting for nearly half of all injuries in some age groups. The NHIS provides an important source of population-based data with which to determine the work relatedness of injuries. Study estimates of days away from work after injury were 1.8 times higher than the Bureau of Labor Statistics (BLS) workplace-based estimates and 1.4 times as high as BLS estimates for private industry. The prominence of occupational injuries among injuries to working-age adults reinforces the need to examine workplace conditions in efforts to reduce the societal impact of injuries.