bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of Grp170 results in catastrophic disruption of endoplasmic reticulum functions
Authors: Mann Melissa J. , Melendez-Suchi Chris , Sukhoplyasova Maria , Flory Ashley R. , Carson Irvine Mary , Iyer Anuradha R. , Vorndran Hannah , Guerriero Christopher J. , Brodsky Jeffrey L. , Hendershot Linda M. , Buck Teresa M. 
Published: October 2023

AbstractGRP170, a product of theHyou1gene, is required for mouse embryonic development, and its ablation in kidney nephrons leads to renal failure. Unlike most chaperones, GRP170 is the lone member of its chaperone family in the ER lumen. However, the cellular requirement for GRP170, which both binds non-native proteins and acts as nucleotide exchange factor for BiP, is poorly understood. Here, we report on the isolation of embryonic fibroblasts from mice in which LoxP sites were engineered in theHyou1loci (Hyou1LoxP/LoxP). A doxycycline-regulated Cre recombinase was also stably introduced into these cells. Induction of Cre resulted in excision ofHyou1and depletion of Grp170 protein, culminating in apoptotic cell death. As Grp170 levels fell we observed increased steady-state binding of BiP to a client, slowed degradation of a misfolded BiP substrate, and BiP accumulation in NP40-insoluble fractions. Consistent with disrupted BiP functions, we observed reactivation of BiP storage pools and induction of the unfolded protein response (UPR) in futile attempts to provide compensatory increases in ER chaperones and folding enzymes. Together, these results provide insights into the cellular consequences of controlled Grp170 loss and insights into mutations in theHyou1locus and human disease.
Chemo-senolytic therapeutic potential against angiosarcoma
Authors: Wang Xuebing , Chung Claire Yik-Lok , Yoshioka Ai , Hashimoto Shinya , Jimbo Haruki , Tanizawa Hideki , Ohta Shinya , Fukumoto Takeshi , Noma Ken-ichi 
Published: October 2023

AbstractAngiosarcoma is an aggressive soft-tissue sarcoma with a poor prognosis. Chemotherapy for this cancer typically employs paclitaxel, one of the taxanes (genotoxic drugs), although it has a limited effect due to chemoresistance for prolonged treatment. Here we examine a new angiosarcoma treatment approach that combines chemotherapeutic and senolytic agents. We first find that the chemotherapeutic drugs, cisplatin and paclitaxel, efficiently induce cellular senescence of angiosarcoma cells. Subsequent treatment with a senolytic agent, ABT-263, eliminates senescent cells through the activation of the apoptotic pathway. In addition, expression analysis indicates that senescence-associated secretory phenotype (SASP) genes are activated in senescent angiosarcoma cells and that ABT-263 treatment eliminates senescent cells expressing genes in the type-I interferon (IFN-I) pathway. Moreover, we show that cisplatin treatment alone requires a high dose to remove angiosarcoma cells, whereas a lower dose of cisplatin is sufficient to induce senescence, followed by the elimination of senescent cells by senolytic treatment. This study sheds light on a potential therapeutic strategy against angiosarcoma by combining a relatively low dose of cisplatin with the ABT-263 senolytic agent, which can help ease the deleterious side effects of chemotherapy.
Adverse mental health inpatient experiences: Qualitative systematic review of international literature and development of a conceptual framework
Authors: Hallett Nutmeg , Dickinson Rachel , Eneje Emachi , Dickens Geoffrey L. 
Published: October 2023

AbstractBackgroundTrauma is increasingly linked to poor health outcomes. Adverse experiences in mental health inpatient settings can be traumatic and contribute to long-lasting negative effects like post-traumatic stress disorder. However, the full range of relevant experiences is often unaddressed in service design and delivery.AimTo describe the spectrum of negative experiences that people identify while they are inpatients in adult mental health services.MethodA systematic literature review was conducted to identify qualitative studies that included people’s subjective negative reports of their inpatient admissions. CINAHL, MEDLINE and PsycINFO were searched from 2000 onwards, alongside a search of Google Scholar. The quality of studies was assessed using the Critical Appraisal Skills Programme qualitative checklist. Data were synthesised using the ‘best-fit’ framework synthesis approach. A patient and public involvement reference group contributed to the review.ResultsStudies (111) from 25 countries were included. Adverse mental health inpatient experiences can be conceptualised under three headings: the ecosystem (the physical environment and the resources available, and other people within or influential to that environment); systems (processes and transitions); and the individual (encroachments on autonomy and traumatisation).ConclusionsImproved patient experience is associated with improved patient outcomes, and addressing negative experiences could significantly impact patient care. Mental health professionals should strive to create inpatient environments that are supportive, respectful, and safe for patients, which consideration of the adversity framework developed from this review can facilitate.
A Systematic Review: Cardiovascular effects of Systemic Sclerosis
Authors: Panchumarthi Dedeepya , Bathula Sasidhar Reddy , madam Sai Tapasvi , Nemani Raghu Ram Shanmukh , Panjiyar Binay Kumar 
Published: October 2023

AbstractSystemic sclerosis is a connective autoimmune disorder characterized by the deposition of an extracellular matrix in the internal organs leading to fibrosis and damage to internal organs. Cardiovascular effects of systemic sclerosis include arrhythmias, coronary artery disease, valvular diseases, pericardial diseases, atherosclerosis, myocardial diseases. Thus it also has a fatal effect on the cardiovascular system making it significant to know its epidemiology, pathophysiology, screening techniques, and investigations which help in early diagnosis of the cardiac lesions of systemic sclerosis. Cardiovascular effects of systemic sclerosis is a multidisciplinary approach by a rheumatologist, cardiologist and radiologist. We reviewed 182 articles from renowned journals taken from google scholar and pubmed published between 2010 and 2023 of which 8 articles are focused for in depth review. This systematic review summarizes the effects of systemic sclerosis on cardiovascular system ranging from arrhythmias to pericardial diseases in brief including the pathogenesis, investigations and epidemiology.
The lung extracellular matrix protein landscape in severe early-onset and moderate chronic obstructive pulmonary disease
Authors: Joglekar M.M. , Bekker N.J. , Ngassie M.L. Koloko , Vonk J.M. , Borghuis T. , Reinders-Luinge M.A. , Bakker J. , Woldhuis R.R. , Pouwels S.D. , Melgert B.N. , Timens W. , Brandsma C.A. , Burgess J.K. 
Published: October 2023

ABSTRACTExtracellular matrix (ECM) remodeling has been implicated in the irreversible obstruction of airways and destruction of alveolar tissue in chronic obstructive pulmonary disease (COPD). Studies investigating differences in the lung ECM in COPD have mainly focused on some collagens and elastin, leaving an array of ECM components unexplored. We investigated the differences in the ECM landscape comparing severe-early onset (SEO-) COPD and moderate COPD to control lung tissue for collagen type I α chain 1 (COL1A1), COL6A1, COL6A2, COL14A1, fibulin 2 and 5 (FBLN2, FBLN5), latent transforming growth factor-beta binding protein 4 (LTBP4), lumican (LUM), versican (VCAN), decorin (DCN), and elastin (ELN) using image analysis and statistical modelling. Percentage area and/or mean intensity of expression of LUM in the parenchyma, and COL1A1, FBLN2, LTBP4, DCN, and VCAN in the airway walls, was proportionally lower in COPD compared to controls. Lowered levels of most ECM proteins were associated with decreasing FEV1measurements, indicating a relationship with disease severity. Furthermore, we identified six unique ECM signatures where LUM and COL6A1 in parenchyma and COL1A1, FBLN5, DCN, and VCAN in airway walls appear essential in reflecting the presence and severity of COPD. These signatures emphasize the need to examine groups of proteins to represent an overall difference in the ECM landscape in COPD, that are more likely to be related to functional effects, than individual proteins. Our study revealed differences in the lung ECM landscape between control and COPD and between SEO and moderate COPD signifying distinct pathological processes in the different subgroups.NEW & NOTEWORTHYOur study identified COPD-associated differences in the lung ECM composition. We highlight the compartmental differences in the ECM landscape in different subtypes of COPD. The most prominent differences were observed for severe-early onset COPD. Moreover, we identified unique ECM signatures that describe airway walls and parenchyma providing insight into the intertwined nature and complexity of ECM changes in COPD that together drive ECM remodeling and may contribute to disease pathogenesis.
ConfluentFUCCI for fully-automated analysis of cell-cycle progression in a highly dense collective of migrating cells
Authors: Goldstien Leo , Lavi Yael , Atia Lior 
Published: October 2023

AbstractUnderstanding mechanisms underlying various physiological and pathological processes requires accurate and fully automated analysis of dense cell populations that collectively migrate, and specifically, relations between biophysical features and cell cycle progression aspects. A seminal tool that led to a leap in real-time study of cell cycle is the fluorescent ubiquitination-based cell cycle indicator (FUCCI). Here, we introduce ConfluentFUCCI, an open-source graphical user interface-based framework designed for fully automated analysis of cell cycle progression, cellular dynamics, and cellular morphology, in highly dense migrating cell collectives. Leveraging state-of-the-art tools, some incorporate deep learning, ConfluentFUCCI offers accurate nuclear segmentation and tracking using FUCCI tags, enabling comprehensive investigation of cell cycle progression at both the tissue and single-cell levels. We compare ConfluentFUCCI to the most recent relevant tool, showcasing its accuracy and efficiency in handling large datasets. Furthermore, we demonstrate the ability of ConfluentFUCCI to monitor cell cycle transitions, dynamics, and morphology within densely packed epithelial cell populations, enabling insights into mechanotransductive regulation of cell cycle progression. The presented tool provides a robust approach for investigating cell cycle-related phenomena in complex biological systems, offering potential applications in cancer research and other fields.
Streamlined generation of CRISPR/Cas9-mediated single-cell knockout clones in murine cell lines
Authors: Tobias Hub , Alex Cornean , Kellen Round , Thomas Fleming , Marc Freichel , Rebekka Medert 
Published: October 2023

AbstractClonal cell lines harbouring loss-of-function mutations in genes of interest are crucial for studying the cellular functions of the encoded proteins. Recent advances in genome engineering have converged on the CRISPR/Cas9 technology to quickly and reliably generate frameshift mutations in the target genes across various cell lines and species. Although high on-target cleavage efficiencies can be obtained reproducibly, screening and identifying clones with loss-of-function alleles remains a major bottleneck. Here, we describe a single sgRNA strategy to generate CRISPR/Cas9-mediated frameshift mutations in target genes of mammalian cell lines that can be easily and cost-effectively identified. Given the proliferation of workhorse cell lines such as HEK293 and N2a cells and the resulting clonal expansion of the cell type, our protocol can facilitate the isolation of knockout clonal cell lines and their genetic validation within a period of down to 3-4 weeks.
Factors affecting merchants' acceptance of P2P m-payments: a multigroup moderating effect of gender, age, and experience
Authors: Jean Pierre Sadrac , Mombeuil Claudel 
Published: October 2023

PurposeThis paper hypothesized that perceived relative advantage and perceived compatibility would have a positive effect on merchants' intention to accept payments via P2P mobile payment services, while perceived financial risks and perceived costs would have a negative effect. The study also explored the differences in gender, age and experience.Design/methodology/approachThe proposed model is based on the valence framework, where positive utility is represented by relative advantage and perceived compatibility, while negative utility is represented by perceived risks and perceived costs. The data for this study were collected from small business owners (merchants) at the largest public market in the Center Department of Mirebalais, Haiti, using a purposive sampling method.FindingsThe results of a structural equation modeling on a sample of 339 merchants only confirmed the effect of both perceived comparative advantage and perceived compatibility. Furthermore, the multigroup analysis revealed that the perceived comparative advantage is stronger for female merchants, older age groups and merchants who frequently used P2P m-payment for the transfer of remittances. Perceived compatibility is stronger for male merchants, younger age groups and merchants who occasionally used P2P m-payment for the transfer of remittances.Originality/valueThis study was conducted in the economic context of Haiti, where P2P m-payments are commonly used for transferring remittances. Since there are limited studies that examine P2P m-payment acceptance from the perspective of merchants, this study offers valuable insights.
Marketing micro-credentials: understanding learners' engagement and willingness to pay more
Authors: Tee Poh Kiong , Cham Tat-Huei , Aw Eugene Cheng-Xi , Khudaykulov Adham , Zhang Xiaoyu 
Published: October 2023

PurposeDigitalisation is reshaping the learning process, shifting it towards personalisation and online learning, and fuelling the growth of massive open online courses (MOOCs) and micro-credentials. Despite being a popular global trend, limited studies have looked at micro-credentials and their impact on learners' behavioural outcomes. The purpose of this study is to investigate the impact of programme design factors on learning experience, as well as the inter-relationships between programme design, learning experience and behavioural responses (e.g. engagement and willingness to pay more (WTPM)) towards micro-credentials. In addition, the study aims to investigate learning enjoyment as a moderator.Design/methodology/approachA survey questionnaire was used to collect data from 354 respondents who are working adults living in the major economic states in Malaysia. Data analysis was performed using the analysis of a moment structures (AMOS) statistical software and SPSS (Statistical Package for the Social Sciences) PROCESS macro.FindingsThe results show the significance of programme design factors (i.e., flexibility, system quality and content quality) in determining the learning experience. The learning experience is found as a mediator in the relationship between programme design factors and learner engagement and WTPM. In addition, the moderation assessment confirms that enjoyment during learning strengthens the relationship between experience and behavioural responses.Originality/valueThis study is amongst a few selected studies that focus on engagement in and WTPM for micro-credentials. In addition, it emphasises the mediating role of the learning experience and the moderating role of enjoyment in understanding the impact of programme design on learners' experiences and behavioural responses.
Social mediametrics: the mention laws and patterns of scientific literature
Authors: Zhao Rongying , Zhu Weijie , Huang He , Chen Wenxin 
Published: October 2023

PurposeSocial mediametrics is a subfield of measurement in which the emphasis is placed on social media data. This paper analyzes the trends and patterns of paper comprehensively mentions on Twitter, with a particular focus on Twitter's mention behaviors. It uncovers the dissemination patterns and impact of academic literature on social media. The research has significant theoretical and practical implications.Design/methodology/approachThis paper explores the fundamental attributes of Twitter mentions by means of analyzing 9,476 pieces of scholarly literature (5,097 from Nature and 4,379 from Science), 1,474,898 tweets and 451,567 user information collected from Altmetric.com database and Twitter API. The study uncovers assorted Twitter mention characteristics, mention behavior patterns and data accumulation patterns.FindingsThe findings illustrate that the top academic journals on Twitter have a wider range of coverage and display similar distribution patterns to other academic communication platforms. A large number of mentioners remain unidentified, and the distribution of follower counts among the mention users exhibits a significant Pareto effect, indicating a small group of highly influential users who generate numerous mentions. Furthermore, the proportion of sharing and exchange mentions positively correlates with the number of user followers, while the incidence of supportive mentions has a negative correlation. In terms of country-specific mention behavior, Thai scholars tend to utilize supportive mentions more frequently, whereas Korean scholars prefer sharing mentions over communicating mentions. The cumulative pattern of Twitter mentions suggests that these occur before official publication, with a half-life of 6.02 days and a considerable reduction in the number of mentions is observed on the seventh day after publication.Originality/valueConducting a multi-dimensional and systematic analysis of Twitter mentions of scholarly articles can aid in comprehending and utilizing social media communication patterns. This analysis can uncover literature's distribution patterns, dissemination effects and social significance in social media.
Characteristics of beech mixed forest distributed at low elevations on Mt. Toishi, northern Kyushu, in the upper warm‐temperate zone of Japan
Authors: Funato Yosuke , Itabashi Koji , Kanetani Seiichi , Sakuta Kotaro 
Published: October 2023

AbstractThe aim of this study was to clarify the structure and regeneration status of mixed beech (Fagus crenata) forest on Mt. Toishi in the Sangun mountain range in northern Kyushu, a relatively low‐latitude region in Japan, with emphasis on forests at lower elevations. We focused on a mixed beech forest at a low elevation of Mt. Toishi and compared the forest structure of the target stands, including tree species and size, with the forest structure near the peaks of Mt. Toishi and Mt. Sangun. Deciduous broad‐leaf trees were dominant near the peaks, while evergreen broad‐leaf trees dominated at lower elevations, whereas the relative dominance of beech trees was not significantly different. Near the peaks, the number of large‐diameter beech trees forming the canopy decreased, and small‐diameter trees were present. The proportion of small‐sized trees was lower in the low‐elevation forest area than in the forest area near the peak. Even at lower elevations, juvenile beech trees grew in limited areas on steep slopes along forest roads. These sites suggested that regeneration of beech populations is possible even at low elevation.
3D superimposition of human dentition contours in personal identification: A preliminary study
Authors: Yu Jiannan , Wang Menglin , Zhou Yu , Jin Xiang , Wang Feng , Sun Jinlong , Hao Wenjun , Yuan Li , Li Yanfeng 
Published: October 2023

AbstractThe human permanent dentition has been commonly used for personal identification due to its uniqueness. Limited research, however, is conducted using 3D digital dental models. We propose to develop a new 3D superimposition method using the contours of human dentition and to further evaluate its feasibility. A total of 270 intraoral scan models were collected from 135 subjects. After a one‐year interval, 52 subjects were chosen at random and the secondary intraoral scan models were obtained. The dentition contours of the first and secondary models were extracted to form a resource dataset and a test dataset. Through the application of the iterative nearest point (ICP) algorithm, the test dataset was registered with the resource dataset, and the root mean square error (RMSE) values of the point‐to‐point distances were calculated. 104 genuine pairs and 13,936 imposter pairs were generated, and in this study, the registration accuracy was 100%. The difference between mean RMSE values for the genuine pair (0.20 ± 0.06 mm) and the minimum RMSE value for the imposter pair (0.83 ± 0.06 mm) was significant in the maxillary arch (p < 0.05). Similarly, in the mandibular arch, the difference between mean RMSE values for the genuine pair (0.22 ± 0.07 mm) and the minimum RMSE value for the imposter pair (0.85 ± 0.08 mm) was significant (p < 0.05). The difference between the RMSE value for the genuine pair in the maxillary and the mandibular arch was significant (p < 0.05). This study indicated the feasibility of dentition contour‐based model superimposition and could be considered for personal identification in the future.
Three‐dimensional lung reconstructions for the localization of lung nodules to be resected during surgery
Authors: Natale Giovanni , Leonardi Beatrice , Messina Gaetana , Bergameo Grazia , Di Filippo Vincenzo , Chisari Giulia , Raciti Gabriele , Lombardo Sofia Paola , Mirra Rosa , Capasso Francesca , Leone Francesco , Pica Davide Gerardo , Fiorelli Alfonso 
Published: October 2023

AbstractBackgroundThe localization of lung nodules is challenging during thoracoscopy. In this study, we evaluated the use of three‐dimensional (3D) lung reconstruction for use in the operating room to guide the identification of lung nodules during thoracoscopy.MethodsThis was a single‐center retrospective study. All consecutive patients undergoing thoracoscopic resection of lung nodules were included in the study. Patients were retrospectively divided into two groups based upon whether the thoracoscopic resection was performed with the assistance (3D group) or not (standard group) of 3D lung reconstruction. The operative time (minutes) to detect lung nodules was statistically compared between the two study groups in relation to the characteristics of lung nodules as size, localization, and distance from the visceral pleura.ResultsOur study population consisted of 170 patients: 85 in the 3D group and 85 in the standard group. No intergroup difference differences were found regarding the characteristics and histological diagnosis of lesions. The standard group compared to the 3D group was associated with a significantly longer operative time for the detection of lesions <10 mm (13.87 ± 2.59 vs. 5.52 ± 1.01, p < 0.001), lesions between 10 and 20 mm (5.05 ± 0.84 vs. 3.89 ± 0.92; p = 0.03), lesions localized in complex segments (7.49 ± 4.25 vs. 5.11 ± 0.97; p < 0.001), and deep lesions (9.58 ± 4.82 vs. 5.4 ± 1.01, p < 0.001).ConclusionsOur 3D lung reconstruction model for use in the operating room may be an additional tool for thoracic surgeons to guide the detection of small and deep nodules during thoracoscopy. It is a noninvasive and cost saving procedure and may be widely used.