bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal role of immune cells in ischemic stroke: a Mendelian Randomization study
Authors: Dong Chenlan , Li Lin , Xiang Xiaolei , Hu Xiaowei , Hu Huiqin , Chu Lisheng 
Published: January 2024

Abstract
Background
Conventional observational designs face challenges in studying this relationship, as confounding factors, reverse causality, minor exposure factors and multiple tests cannot be completely eliminated. There is currently a lack of MR studies concerning immune cells and the risk of ischemic stroke. This particular study offers a novel perspective on risk prediction for ischemic stroke.
Objective
To investigate the causal relationship between immune cells and ischemic stroke through Mendelian randomization analysis.
Methods
A complete two-sample Mendelian randomization (MR) analysis was utilized to ascertain the causative relationship between immune cells and ischemic stroke. Using publicly available genetic data, we investigated the causal association between 731 immune cells and the risk of ischemic stroke. Four immune characteristics were included: relative cells (RC), absolute cells (AC), median fluorescence intensity (MFI), and morphological parameters (MP). MR-Egger, Weighted median, Inverse variance weighted (IVW), Weighted mode, Simple mode, and MRPRESS were utilized for analysis. Heterogeneity and horizontal pleiotropy tests were also conducted.
Results
Mendelian randomization analysis showed that 32 of the 731 immune cells had a robust causal relationship with ischemic stroke, among which 15 immune cells such as IgD−CD27− %B cell (β = 0.033, 95%CI = 1.002 ~ 1.065, p = 0.037), IgD+ CD24 + AC (β = 0.045, 1.010 ~ 1.082, p = 0.012), CD25hi CD45RA−CD4 not Treg %T cell (β = 0.022, 95%CI = 1.002 ~ 1.042, p = 0.028) and soon. CD62L−HLADR++ monocyte AC (β =-0.053, 95% CI = 0.914 ~ 0.985, p = 0.005), CD33br HLA DR+ CD14− AC (β =-0.017, 95% CI = 0.972 ~ 0.995, p = 0.004), EM DN (CD4−CD8−) %DN (β =-0.014, 95% CI = 0.975 ~ 0.997, p = 0.014), etc. There exists a strong inverse causal link for ischemic stroke.
Conclusion
Our study has demonstrated a close genetic link between immune cells and ischemic stroke. Fifteen immune cells such as IgD−CD27− %B cell, IgD+ CD24+ AC, CD25hi CD45RA−CD4 not Treg %T cell have robust positive causal associations with ischemic stroke, and seventeen immune cells such asCD62L− HLA DR++ monocyte AC, CD33br HLA DR+ CD14− AC, EM DN (CD4−CD8−) %DN have robust positive causal associations with ischemic stroke. A strong inverse causal relationship with ischemic stroke offers direction for forthcoming clinical studies.
Transcriptomics and gut microbiome analysis of the edible herb Bidens pilosa as a functional feed additive to promote growth and metabolism in tilapia (Oreochromis spp.)
Authors: Chen Che-Chun , Lin Chung-Yen , Lu Hsin-Yun , Liou Chyng-Hwa , Ho Ying-Ning , Huang Chang-Wen , Zhang Zhong-Fu , Kao Chih-Hsin , Yang Wen-Chin , Gong Hong-Yi 
Published: January 2024

Abstract

Background
To reduce the use of antibiotics and chemicals in aquaculture, an edible herb - B. pilosa - has been selected as multifunctional feed additives to address this issue. Although there has been considerable research into the effects of B. pilosa on poultry, the wider effects, particularly on the growth and gut microbiota in fish, remain largely unexplored. We aim to investigate the interactive effects between the host on growth and the gut microbiota using transcriptomics and gut microbiota in B. pilosa-fed tilapia.
Results
In this study, we added 0.5% and 1% B. pilosa to the diet and observed that the growth performance of tilapia was significantly increased after 8 weeks of feeding. Comparative transcriptome analysis was performed on RNA sequence profiles obtained from liver and muscle tissues. Functional enrichment analysis showed that B. pilosa regulates several pathways and genes including amino acid metabolism, lipid metabolism, carbohydrate metabolism, endocrine system, signal transduction and metabolism of other amino acids. The expression of selected growth-associated genes was validated by qRT-PCR. The qRT-PCR result indicated that B. pilosa may enhance growth performance by activating the expression of liver igf1 and muscle igf1rb genes and inhibiting the expression of the muscle negative regulator myostatin b (mstnb). Enhancement of endocrine Igf1/Igf1rb signaling and suppression of Mstn signaling both induced the expression of myogenic regulatory factors (MRFs), myod1, myogenin and mrf4, to promote muscle growth in tilapia. The predicted function of the gut microbiota showed several significantly different pathways that overlapped with the KEGG enrichment results of differentially expressed genes in the liver transcriptomes. This suggests that gut microbiota may be able to influence liver metabolism through the gut-liver axis in B. pilosa-fed tilapia.
Conclusions
In conclusion, dietary B. pilosa can regulate endocrine igf1 signaling and myostatin signaling to activate expression of MRFs to promoter muscle growth, and alter the composition of gut bacteria, which can then affect liver amino acid metabolism, carbohydrate metabolism, the endocrine system, lipid metabolism, metabolism of other amino acids, and signal transduction of the host, ultimately enhancing growth performance. Our results suggest that B. pilosa has the potential to be a functional additive that can be an alternative to reduce antibiotics as growth promoter in aquaculture organisms.
Morphological features of the eyelid margin in relation to conjunctival goblet cells and MUC5AC in tear film and clinical tests of dry eye
Authors: Dou Zexia , Sun Ming , Huang Yue , Zhao Shaozhen 
Published: January 2024

Abstract
Purpose This study aimed to investigate the morphological features of eyelid margin in dry eye patients and determine their correlations with conjunctival goblet cells, MUC5AC secretion, and clinical tests of tear film.
Methods Patients diagnosed with dry eye disease (DED) were asked to complete the evaluations of symptoms and signs of dry eye, including Ocular Surface Disease Index (OSDI) questionnaire, Lipid layer thickness (LLT), partial blinking (PB) rate, tear-film breakup time (TBUT), Schirmer’s I test (SIt), and meiboscore. Marx line (ML), lid wiper epitheliopathy (LWE), and meibomian orifices (MOs) were used as indicators of morphological features of the eyelid margin. PAS staining of conjunctival goblet cells and MUC5AC measurement of the tear samples were also performed.
Results A total of 30 patients diagnosed with DED were included. The ML score was correlated with the SIt. The MOs showed a significant negative correlation with the TBUT and LLT. The LWE was corrected to TBUT and meiboscore, and also associated with decreased conjunctival goblet cell density and MUC5AC concentration in the tear film rather than ML and MOs in the eyelid margin of dry eye patients. The tear MUC5AC concentration had a significant positive correlation with conjunctival goblet cell density.
Conclusion The eyelid margin is crucial for maintaining the integrity of tear film and ocular surface. Attracting more attention to the eyelid margin is beneficial to routine clinical practice, and lid wipers could be used to evaluate the secretion of MUC5AC in the tear film secreted by conjunctival goblet cells.
The study of human mRNA m7G methylation writer molecule related lncRNA in gastric cancer
Authors: Ma Tianyu , Zhou Dongli , Hu Binbin 
Published: January 2024

Abstract
OBJECTIVE
Presenting a poor prognosis, gastric cancer (GC) remains one of the leading causes of disease and death worldwide. N7-methylguanosine (m7G) is the most prevalent RNA modification. m7G-long noncoding RNAs (lncRNAs) regulate GC onset and progression, but their precise mechanism in GC is unclear.
MATERIALS AND METHODS
The Cancer Genome Atlas database helped extract gene expression data and clinical information for GC. Coexpression analysis helped identify m7G-related lncRNAs. Univariate Cox and multivariate Cox analyses helped identify m7G-related lncRNAs with predictive capability. The chi square test was used to study the relationship between the expression of lncRNAs and clinical characteristics. Enrichment analysis (GSEA) was used for pathway enrichment analysis.
RESULTS
Thirty-one m7G lncRNAs were identified by coexpression analysis. Further univariate Cox and multivariate Cox analyses were used to screen the prognostic lncRNAs. There is only one lncRNA, REPIN1 antisense RNA 1 (REPIN1-AS1), that can be used as an independent prognostic factor. Therefore, REPIN1-AS1 was selected as the molecule for further study. Then, REPIN1-AS1 was enriched by GSEA. Finally, drug sensitivity was analysed.
CONCLUSIONS
We constructed a m7G-related lncRNA prognostic model, and the results showed that REPIN1-AS1 could be an independent prognostic factor involved in tumour formation and other signalling pathways and had potential therapeutic targets.
VERSA: Validating Evaluations with a Robust Scoring Approach - Detecting Fake Reviews through MRCS
Authors: Ennaouri Mohammed , Zellou Ahmed 
Published: January 2024

Abstract
The emergence of online commerce in the digital age resulted to an unparalleled dependence on consumer feedback for making informed choices. However, the rise in the importance of reviews has coincided with an increase in fraudulent behavior, as fake reviews have come into platforms and influenced users' opinions. The widespread use of these deceptive reviews has become an increasing issue, reducing the trust that is essential for the success of online marketplaces.
This study uses a comprehensive approach to handle the difficulty of identifying fake reviews. This distinctive strategy introducing a multidimensional methodology incorporating advanced Natural Language Processing (NLP) tools. The utilization of Word2Vec for semantic richness in review analysis is detailed, emphasizing its ability to capture contextual complexities. The integration of new metric navigates the semantic landscape, offering a normalized measure of textual closeness critical for precise detection. A distinctive feature is the incorporation of an exaggeration detection method, which improves the model's capacity to reveal misleading complexities in reviews' language. Moreover, an adaptive clustering is elaborated, which deviates from conventional K-means clustering by continuously adjusting clusters to account for the dynamic nature of deceptive language patterns.
The approach’s excellent accuracy is demonstrated via empirical validation on the Yelp Labeled Dataset, which also highlights areas for development. Metrics like precision, recall, and F1 score provide light on the model's advantages and direct further improvements. The conclusion examines how misleading reviews affect digital trust more broadly and highlights the proposed approach as a critical first step in reducing fraudulent activity.
Study of short-term periodicities in the occurrence of Forbush decreases: Wavelet analysis
Authors: Singh Y. P. , Badruddin Badruddin 
Published: January 2024

Abstract
We utilized the Forbush decreases (magnitude >1.5%) detected in cosmic ray neutron monitor data during continuous five solar cycles, viz., 20, 21, 22, 23 and 24 (1965 to 2019) and subjected them to wavelet analysis in order to obtain the possible periodicities in their occurrence. We also studied the periodicities separately during the odd and even solar activity cycles. In addition to solar activity, the solar magnetic polarity and its extension into the interplanetary space makes significant difference in the cosmic ray modulation in the helisphere, we have also applied the wavelet analysis procedure separately during positive (A > 0) and negative (A < 0) polarity states of the heliospheric magnetic fields. Observed periodicities in Forbush decreases have been discussed and compared with earlier detected periodicities in solar and geomagnetic activity indices, e.g., sunspot numbers, sunspot areas, sunspot groups, solar flares, coronal mass ejections, and various geomagnetic activity indices. Significant short-term periodic behaviour detected in the occurrence of Forbush decreases, which in general, corroborates the observed behaviour in solar (in particular, solar eruptive activity) and geomagnetic activity. Understanding the quasi-periodic process in magnetic field emergence from solar active regions and solar eruptive activity, as well as solar-terrestrial coupling and space weather effects, requires comparing the quasi-periodic behaviour between parameters representing solar and geomagnetic activity along with cosmic ray variability.
Comprehensive analysis of LD-related genes signature for predicting prognosis and immunotherapy response in clear cell renal cell carcinoma
Authors: Yangtao Jia , Xinke Dong , Fangzheng Yang , Huimin Long , Libin Zhou 
Published: January 2024

Abstract

Background
Lipid droplets (LD) in renal clear cell carcinoma (ccRCC)play a crucial role in lipid metabolism and immune response modulation. The purpose of this study was to create a LD-related signature to predict prognosis and guide the immunotherapy and targeted therapy in ccRCC patients.
Methods
We conducted a comprehensive analysis using transcriptional profiles and clinical data obtained from The Cancer Genome Atlas (TCGA). LD-related genes were identified from existing literature and the GeneCards database, and differentially expressed genes were determined. Sequentially, we conducted Cox regression analysis and Lasso regression analysis, to establish a prognostic risk model. The performance of the risk model was evaluated using Kaplan-Meier (KM) analysis and time-dependent receiver operating characteristic (ROC) analysis. Additionally, gene set enrichment analysis (GSEA), ESTIMATE, CIBERSORT, and immunophenoscore (IPS) algorithm were used to assess the tumor microenvironment (TME) and treatment response.
Results
We constructed a risk signature with four LD-related genes in the TCGA dataset, which could be an independent prognostic factor in ccRCC patients. Then, patients were classified into two risk groups and exhibited notable differences in overall survival (OS), progression-free survival (PFS), and TME characteristics. Furthermore, we developed a comprehensive nomogram based on clinical features, which demonstrated good prognostic predictive value. According to the results of GSEA analysis, immune-related pathways were found to be significantly enriched in the high-risk group. Additionally, the high-risk group displayed high levels of immune cell infiltration, TMB and IPS scores, indicating better efficacy of immune checkpoint inhibitors (ICIs). Finally, high-risk demonstrated reduced IC50 values compared to the low-risk counterpart for specific targeted and chemotherapeutic drugs., suggesting that the patients receiving these targeted drugs in high-risk group had better treatment outcomes.
Conclusions
Our LD-related genes signature provides reliable prognostic predictions and can guide immunotherapy and targeted therapy in ccRCC patients.
Safety Diagnostic Tool for Non-Small Cell Lung Cancer (NSCLC) Lyophilized Serum
Authors: Mohammed Mohammed , Mohammed Asmaa M S 
Published: January 2024

Abstract
Background and Objectives
The international protocol used to diagnose non-small cell lung cancer (NSCLC) usually faces an inappropriate result due to the poor diagnostic ability in the early stages. Carcinoembryonic Antigen (CEA), an established serum tumor marker that is used for NSCLC diagnosis, has limited sensitivity and specificity, but, still, it is the predominant complementary detecting tool wherein its results confirm diagnostic radiology findings (PET-CT). Unfortunately, the limited range of its sensitivity is unable to classify approximately one third of patients suffering from NSCLC. Due to a huge number of patients lately classified as NSCLC, the efficacy of the offered treatment is limited. Hence, the importance of discovering, improving, and establishing a new technique that participates in the NSCLC diagnosis is indeed urgent.
Methods
The low angle x-ray scattering (LAXS) technique was applied on the lyophilized serum of NSCLC patients to create patient profiles that were able to distinguish the molecular differences between NSCLC patients, avoiding the undesirable radiation exposure to the patients.
Results
The created LAXS profile was characterized by two peaks. The first scattering peak at 4.8° was sensitive to molecular alterations in protein structures that were the main characteristic differences from the normal serum. Comparing the measurements of LAXS profiles of NSCLC with the normal sera, the unique first scattering peak at 4.8° was elucidated as a characterization shape and profile for NSCLC and normal individuals. Conclusions: Using the LAXS technique gives us full details at a molecular level that are introduced as a promising tool that could be a supporter in NSCLC early detection.
On the characteristics of aphantasia, hyperphantasia and sensory imagery in a multi-cultural sample
Authors: Bruder Jennifer , Zehra Masooma 
Published: January 2024

Abstract
Sensory imagery, such as visual imagery, is assumed to be a universal cognitive ability, yet a current research gap is a lack of cultural diversity in the existing literature. Further, research has primarily focused on visual imagery, as opposed to other sensory imagery. Visual imagery presents with a large degree of individual variability and includes two extreme forms, aphantasia and hyperphantasia. This study investigated sensory imagery in a multi-cultural sample. Participants (N = 636) completed the Vividness of Visual Imagery Questionnaire (VVIQ) scale and the Plymouth Sensory Imagery Questionnaire (Psi-Q). Altogether 5.0% of participants fell within the aphantasia range (VVIQ: 16–32) and 12.1% within the hyperphantasia range (VVIQ: 75–80). Across the sample, no VVIQ differences were found for gender, age, occupation, or education. VVIQ scores positively correlated with all imagery scales in the Psi-Q, suggesting imagery across senses is not independent. To explore cultural differences, participants were divided into five cultural groups: 1) Western Educated Industrialized Rich & Democratic (WEIRD) (n = 89); 2) South-East Asia (SEA, n = 121); 3) South Asia (n = 180); 4) Middle East and North Africa (MENA, n = 86), and 5) Arabian Gulf (Arab, n = 43). WEIRD and SEA participants reported significantly higher levels of visual imagery in the VVIQ than those from MENA. Within the Psi-Q sensory subscales, SEA and WEIRD participants reported significantly higher imagery than MENA and Arabian Gulf participants. In conclusion, this study contributes to previous prevalence reports for aphantasia and hyperphantasia in a novel sample and demonstrates the need to consider culture when investigating imagery across all senses.
Effect of Humidity on of Sol-Gel Glass Chirowaveguide Thin Films and Microstructures
Authors: SAHIB Hoshang , Bensalah-Ledoux Amina , Gassenq Alban , Baguenard Bruno , Guy Laure , Guy Stephan 
Published: January 2024

Abstract
We present a comprehensive study on the effect of humidity on organically modified sol-gel glasses (ormosils) thin films and related-microstructures, using optical characterization methods.Using a Mach-Zehnder interferometer and a polarimetric setup, we quantitatively measure the variations in refractive index and linear birefringence induced by changes in ambient relative humidity. Our findings unveil the high sensitivity of the material's refractive index to humidity, predominantly attributed to the hygroscopic nature of the polyethylene glycol (PEG) component embedded within the ormosils.Furthermore, We show that stress-induced birefringence during the deposition of the sol-gel layers is partially released in the structured layers compared to the planar one. The remaining stress is reduced by the effect of humidity in a linear relationship in which the proportionality factor is higher in the planar layers. The results shown here shed new light on the complex relationship between humidity and sol-gel derived materials and contribute to the understanding of the mechanisms behind their sensitivity to humidity.
The January 1 st 2024 Noto Peninsula Co-seismic Landslides Hazards: Preliminary Results
Authors: Gomez Christopher 
Published: January 2024

Abstract
On the first day of 2024, a strong Mw.7.6 earthquake followed by a tsunami shook the Noto Peninsula (Japan) located on the coast facing the Sea of Japan. It resulted in numerous casualties, infrastructures and dwelling destroyed. The earthquake also triggered at least 930 coastal and mountain co-seismic landslides that were digitized from the emergency aerial photographs (2/1/2024), in order to provide a preliminary assessment of their distribution and characteristics. The medium surface of the landslides was found to be 1749 m2, with numerous small < 50 m2 landslide and at least one large deep-seated landslide (0.8 km x 1 km). The mountain landslides were concentrated around two clusters, which were not close to the epicentre, but around 7 km and 10 km from the epicentre. From a disaster perspective, the 1/1/2024 Noto Peninsula earthquake is typical of a ‘coastal earthquake’ where the coastal landslides, even sparse collapsed on the main artery of the peninsula, the ring road, isolating communities and hampering the disaster relief process.
Contemporary Survival Outcomes and Prognostic Factors in Glioblastoma: An Institutional Analysis
Authors: Narang Kushal , Kataria Tejinder , Bisht Shyam Singh , Gupta Deepak , Banerjee Susovan , Mayank Mayur , Shishak Sorun , Kaliyaperumal Venkatesan , Tamilselvan S , Kamaraj Dayanithi , Abraham Susan 
Published: January 2024

Abstract
Purpose
There have been significant advancements in diagnostic radiology and pathology, and in the technical aspects of surgery, radiation therapy and temozolomide used for treatment of glioblastoma. We analyzed the outcomes of 267 adult patients with glioblastoma, consecutively treated between December 2010 and November 2018 using modern techniques at our institute.
Methods
All patients underwent surgical resection, volumetric modulated arc therapy-based radiation therapy using institute-specific target-delineation guidelines and image-guidance, and temozolomide according to Stupp’s protocol. Serial multiparametric magnetic resonance imaging-based follow-up ensured early detection of disease progression. Appropriate salvage therapy was determined based on clinicopathological attributes. Kaplan-Meier survival plots, log-rank test and Cox regression analysis was performed on the prospectively recorded dataset to estimate survival, and the factors affecting it.
Results
The median progression-free survival (PFS), 1-year PFS, and 2-year PFS were 10 months, 37.8%, and 17.5%, respectively. Radiation dose ≥ 54 Gy and ≥ 4 adjuvant TMZ cycles were associated with favourable PFS. The median overall survival (OS), 2-year OS and 5-year OS were 24 months, 48% and 18%, respectively. One-year disease control, surgery-use at progression, and systemic therapy-use at progression were associated with favourable OS. Haematological toxicity ≥ Grade 2 was evident in 6% patients during concurrent radiation-temozolomide phase, and in 9% patients in adjuvant temozolomide phase. Post-radiation neurocognitive deficits were noted in 20.1% patients, with onset at a median duration of 10 months.
Conclusion
Modern diagnostic and therapeutic techniques for glioblastoma effected a near-doubling of survival compared to historical data, with acceptable late toxicity.
B-Index as activity proxy for Proxima Centauri star ; HARPS survey
Authors: Azizi Fatemeh , Mirtorabi Mohammad Taghi , Foroughi Rahimeh 
Published: January 2024

Abstract

Stellar activity cycles on magnetically active stars can be estimated by molecular absorption bands.A new color indicator which compares absorptional line strength of the T iOλ567nm with its near continuum has previously been introduced. In this work we use this indicator to evaluation activity cycle for Proxima Centauri star, using spectroscopic data from HARPS survey. The results indicate periodicity with an activity period of 2873+47.4 −53.9 days.