bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gentle label-free nonlinear optical imaging relaxes linear-absorption-mediated triplet
Authors: Wang Geng , Li Lianhuang , Sorrells Janet E. , Chen Jianxin , Tu Haohua 
Published: October 2023

AbstractSample health is critical for live-cell fluorescence microscopy and has promoted light-sheet microscopy that restricts its ultraviolet-visible excitation to one plane inside a three-dimensional sample. It is thus intriguing that laser-scanning nonlinear optical microscopy, which similarly restricts its near-infrared excitation, has not broadly enabled gentle label-free molecular imaging. We hypothesize that intense near-infrared excitation induces phototoxicity via linear absorption of intrinsic biomolecules with subsequent triplet buildup, rather than the commonly assumed mechanism of nonlinear absorption. Using a reproducible phototoxicity assay based on the time-lapse elevation of auto-fluorescence (hyper-fluorescence) from a homogeneous tissue model (chicken breast), we provide strong evidence supporting this hypothesis. Our study justifies a simple imaging technique, e.g., rapidly scanned sub-80-fs excitation with full triplet-relaxation, to mitigate this ubiquitous linear-absorption-mediated phototoxicity independent of sample types. The corresponding label-free imaging can track freely movingC. elegansin real-time at an irradiance up to one-half of water optical breakdown.
In vitroreconstitution reveals membrane clustering and RNA recruitment by the enteroviral AAA+ ATPase 2C
Authors: Shankar Kasturika , Sorin Marie N. , Sharma Himanshu , Skoglund Oskar , Dahmane Selma , Ter Beek Josy , Tesfalidet Solomon , Nenzén Louise , Carlson Lars-Anders 
Published: October 2023

AbstractEnteroviruses are a vast genus of positive-sense RNA viruses that cause diseases ranging from common cold to poliomyelitis and viral myocarditis. They encode a membrane-bound AAA+ ATPase, 2C, that has been suggested to serve several roles in virus replication, e.g. as an RNA helicase and capsid assembly factor. Here, we report the reconstitution of full-length, poliovirus 2C’s association with membranes. We show that the N-terminal membrane-binding domain of 2C contains a conserved glycine, which is suggested by structure predictions to divides the domain into two amphipathic helix regions, which we name AH1 and AH2. AH2 is the main mediator of 2C oligomerization, and is necessary and sufficient for its membrane binding. AH1 is the main mediator of a novel function of 2C: clustering of membranes. Cryo-electron tomography reveal that several 2C copies mediate this function by localizing to vesicle-vesicle interfaces. 2C-mediated clustering is partially outcompeted by RNA, suggesting a way by which 2C can switch from an early role in coalescing replication organelles and lipid droplets, to a later role where 2C assists RNA replication and particle assembly. 2C is sufficient to recruit RNA to membranes, with a preference for double-stranded RNA (the replicating form of the viral genome). Finally, thein vitroreconstitution revealed that full-length, membrane-bound 2C has ATPase activity and ATP-independent, single-strand ribonuclease activity, but no detectable helicase activity. Together, this study suggests novel roles for 2C in membrane clustering, RNA membrane recruitment and cleavage, and calls into question a role of 2C as an RNA helicase. The reconstitution of functional, 2C-decorated vesicles provides a platform for further biochemical studies into this protein and its roles in enterovirus replication.
Ultrahigh throughput evolution of tryptophan synthase in droplets via an aptamer-biosensor
Authors: Scheele Remkes A. , Weber Yanik , Nintzel Friederike E. H. , Herger Michael , Kaminski Tomasz S. , Hollfelder Florian 
Published: October 2023

AbstractTryptophan synthase catalyzes the synthesis of a wide array of non-canonical amino acids and is an attractive target for directed evolution. Droplet microfluidics offers an ultrahigh throughput approach to directed evolution (>107experiments per day), enabling the search for biocatalysts in wider regions of sequence space with reagent consumption minimized to the picoliter volume (per library member). While the majority of screening campaigns in this format on record relied on an optically active reaction product, a new assay is needed for tryptophan synthase. Tryptophan is not fluorogenic in the visible light spectrum and thus falls outside the scope of conventional droplet microfluidic read-outs which are incompatible with UV light detection at high throughput. Here, we engineer a tryptophan DNA aptamer into a biosensor to quantitatively report on tryptophan production in droplets. The utility of the biosensor was validated by identifying 5-fold improved tryptophan synthases from ∼100,000 protein variants. More generally this work establishes the use of DNA-aptamer sensors with a fluorogenic read-out in widening the scope of droplet microfluidic evolution.
A human prenatal skin cell atlas reveals immune cell regulation of skin morphogenesis
Authors: Gopee Nusayhah Hudaa , Huang Ni , Olabi Bayanne , Admane Chloe , Botting Rachel A. , Foster April Rose , Torabi Fereshteh , Winheim Elena , Sumanaweera Dinithi , Goh Issac , Miah Mohi , Stephenson Emily , Tun Win Min , Moghimi Pejvak , Rumney Ben , He Peng , Lawrence Sid , Roberts Kenny , Sidhpura Keval , Englebert Justin , Jardine Laura , Reynolds Gary , Rose Antony , Ganier Clarisse , Rowe Vicky , Pritchard Sophie , Mulas Ilaria , Fletcher James , Popescu Dorin-Mirel , Poyner Elizabeth , Dubois Anna , Filby Andrew , Lisgo Steven , Barker Roger A. , Park Jong-Eun , Vento-Tormo Roser , Le Phuong Ahn , Serdy Sara , Kim Jin , Deakin CiCi , Lee Jiyoon , Nikolova Marina , Rajan Neil , Ballereau Stephane , Li Tong , Moore Josh , Horsfall David , Lozada Daniela Basurto , O’Toole Edel A. , Treutlein Barbara , Bayraktar Omer , Kasper Maria , Mazin Pavel , Gambardella Laure , Koehler Karl , Teichmann Sarah A. , Haniffa Muzlifah 
Published: October 2023

SummaryHuman prenatal skin is populated by innate immune cells including macrophages, and whether they act solely in immunity or have additional functions in morphogenesis is unclear. We assembled the first comprehensive multi-omic reference atlas of prenatal human skin (7-16 post-conception weeks), combining single cell and spatial transcriptomic data, to characterise the skin’s microenvironmental cellular organisation. This revealed that crosstalk between non-immune and immune cells underpins formation of hair follicles, has implications for scarless wound healing, and is critical for skin angiogenesis. We benchmarked a skin organoid model, derived from human embryonic stem (ES) and induced pluripotent stem (iPS) cells, against prenatal and adult skin, demonstrating close recapitulation of the epidermal and dermal skin components during hair follicle development. Notably, the skin organoid lacked immune cells and had markedly diminished endothelial cell heterogeneity and quantity. From ourin vivoskin cell atlas data, we found that macrophages and macrophage-derived growth factors play a key role in driving endothelial development prenatally. Indeed, vascular network formation was enhanced following transfer of autologous iPS-derived macrophages into both endothelial cell angiogenesis assays and skin organoid cultures. In summary, innate immune cells moonlight as key players in skin morphogenesis beyond their conventional immune roles, a function they achieve via extensive crosstalk with non-immune cells. Finally, we leveraged our human prenatal skin cell atlas to further our understanding of the pathogenesis of genetic hair and skin disorders.
Automatic simulated dental implants method with implant parameter estimation based on CBCT images
Authors: Wang Kai , Liu Jiaying , Yang Shuai , Guo Jing , Ke Yongzhen 
Published: October 2023

PurposeThis paper aims to automatically obtain the implant parameter from the CBCT images to improve the outcome of implant planning.Design/methodology/approachThis paper proposes automatic simulated dental implant positioning on CBCT images, which can significantly improve the efficiency of implant planning. The authors introduce the fusion point calculation method for the missing tooth's long axis and root axis based on the dental arch line used to obtain the optimal fusion position. In addition, the authors proposed a semi-interactive visualization method of implant parameters that be automatically simulated by the authors' method. If the plan does not meet the doctor's requirements, the final implant plan can be fine-tuned to achieve the optimal effect.FindingsA series of experimental results show that the method proposed in this paper greatly improves the feasibility and accuracy of the implant planning scheme, and the visualization method of planting parameters improves the planning efficiency and the friendliness of system use.Originality/valueThe proposed method can be applied to dental implant planning software to improve the communication efficiency between doctors, patients and technicians.
Board gender diversity and corporate litigation: evidence from China
Authors: Zheng Xiaojing , Wang Xiaoxian 
Published: October 2023

Purpose
This study aims to examine the effect of board gender diversity on corporate litigation in China’s listed firms. The key questions this study addresses are: what are the effect of board gender diversity on corporate litigation in terms of both the frequency and severity of consequence, is there any heterogeneous effects of the relationships across firm performance?

Design/methodology/approach
A sample consists of 25,668 firm-year observations from over 3,340 firms is examined using logistic regression analysis and negative binomial regression analysis. The authors also use event study method and ordinary least square (OLS) regression to explore female directors’ effects on reducing the negative consequences of litigation. The logistic regression and OLS regression are reestimated with interaction terms when examining the firm performance heterogeneity.

Findings
The authors document that firms with greater female representation on their boards experience fewer and less severe corporate litigations. Moreover, in high-performing firms, board gender diversity plays a more potent role in reducing the frequency and consequences of corporate litigation than low-performing firms.

Originality/value
This study is among the first to examine the relationship between board gender diversity and the comprehensive corporate litigations under Chinese context. It sheds new light on China’s boardroom dynamics, offering valuable empirical implication to Chinese corporate policymakers on the role of female directors.
The readability and narrative tone of risk and risk management disclosures for South African listed companies
Authors: Enslin Zack , du Toit Elda , Puane Mangwakong Faith 
Published: October 2023

PurposeRisk information provides information to enable stakeholders to make informed decisions about a company. Corporate communications should be readable and unbiased so as not to hamper disclosure usefulness. This study assesses whether risk disclosures in the integrated reports are readable and unbiased.Design/methodology/approachThe readability and narrative tone of South African listed companies' risk and risk management disclosures as disclosed in their integrated reports are analysed using automated software for the Top 40 JSE listed companies from 2015 to 2019.FindingsThe results show that risk and risk management disclosures are unreadable and lack any improvement in readability during the period. Additionally, these disclosures are biased toward narrative tones signalling communality and certainty.Originality/valueThe study adds to the literature on the readability of corporate reports, by focussing on the readability and narrative tone of risk and risk management disclosures during a period of increased scrutiny over the content of such disclosures. Also, by analysing risk disclosure and risk management disclosure separately, and by performing trend analysis to determine whether requirement changes related to content (specifically King IV) affect readability and narrative tones.
Recent developments in entrepreneurial marketing: systematic literature review, thematic analysis and research agenda
Authors: Breit Luca A. , Volkmann Christine K. 
Published: October 2023

Purpose
The developing field of entrepreneurial marketing reflects input from both marketing and entrepreneurship. Since the early 1980s, it has evolved heterogeneously, without a coherent theory, leading to complex scholarly views. Therefore, this literature review aims to shed light on the recent developments, reveal various research perspectives related to entrepreneurial marketing and derive future research avenues.

Design/methodology/approach
To account for recent scientific contributions and establish a more transparent view of divergent insights, the systematic literature review reported herein covers 207 peer-reviewed journal articles published after the “Charleston Summit” over 12 years (2010–2021) and details their contributions based on descriptive and inductive thematic analysis.

Findings
First, a descriptive analysis illustrates recent scientific developments indicating that entrepreneurial marketing is a vibrant research field with a continuous increase in publications worldwide and a wide range of research methods applied. Second, the thematic analysis suggests a three-part classification into entrepreneur, business and market perspectives. The authors present the most frequent themes and subthemes within this literature domain, as well as offering a critical assessment of the field that reveals key directions for expanding existing research.

Originality/value
To the best of the authors’ knowledge, this study is the first comprehensive review systematically examining entrepreneurial marketing literature while conducting an in-depth thematic analysis. It enhances current knowledge of the field by extending previous narrative and bibliographic reviews and discussing research directions. Aside from specific research questions, an alternative way to narrow down the multiple research objects is elaborated by critically debating the perspectives.
Personal touch in digital customer service: a conceptual framework of relational personalization for conversational AI
Authors: Blümel Jan Hendrik , Zaki Mohamed , Bohné Thomas 
Published: October 2023

PurposeCustomer service conversations are becoming increasingly digital and automated, leaving service encounters impersonal. The purpose of this paper is to identify how customer service agents and conversational artificial intelligence (AI) applications can provide a personal touch and improve the customer experience in customer service. The authors offer a conceptual framework delineating how text-based customer service communication should be designed to increase relational personalization.Design/methodology/approachThis paper presents a systematic literature review on conversation styles of conversational AI and integrates the extant research to inform the development of the proposed conceptual framework. Using social information processing theory as a theoretical lens, the authors extend the concept of relational personalization for text-based customer service communication.FindingsThe conceptual framework identifies conversation styles, whose degree of expression needs to be personalized to provide a personal touch and improve the customer experience in service. The personalization of these conversation styles depends on available psychological and individual customer knowledge, contextual factors such as the interaction and service type, as well as the freedom of communication the conversational AI or customer service agent has.Originality/valueThe article is the first to conduct a systematic literature review on conversation styles of conversational AI in customer service and to conceptualize critical elements of text-based customer service communication required to provide a personal touch with conversational AI. Furthermore, the authors provide managerial implications to advance customer service conversations with three types of conversational AI applications used in collaboration with customer service agents, namely conversational analytics, conversational coaching and chatbots.
Connecting managers’ international work experience, advice networks, and subsidiary-unit performance: a social capital perspective
Authors: Kim Taehyung , Chung Chul , Brewster Chris , Yoon Sang-Hyeak 
Published: October 2023

Purpose
This study aims to examine whether and why subsidiary-unit managers’ prior international work experiences across multinational enterprises’ (MNEs) home and host countries impact their subsidiary-unit performance, considering the mediating effect of their advice networks.

Design/methodology/approach
A survey on 222 subsidiary-unit managers (154 parent country nationals [PCNs] and 68 host country nationals [HCNs]) of a Korean MNE operating in China, Vietnam, Thailand, Singapore, Hungary and Slovakia was conducted. The authors analyzed the data using partial least square structural equation modeling, multigroup analysis and bootstrapping techniques.

Findings
PCN subsidiary managers with more prior international work experience manage better-performing units due to the strength of the manager’s advice networks across local parties. However, for HCN subsidiary managers, this study did not find such mediating roles of the size and strength of their advice networks in the MNE home country.

Originality/value
This study provides novel insights and empirical evidence about the effect of the length of prior international work experience of subsidiary managers on their advice-seeking networks and subsidiary-unit performance. In addition, it draws on and add to social capital theory about how international work experience impacts dealing with local businesses and the relationship with corporate headquarters.
Development of a textile multilayered device with piezoelectric property using different knitted fabric structures
Authors: Maestri Gabriela , Merlini Claudia , Mejia Leonardo , Steffens Fernanda 
Published: October 2023

Purpose
This study aims to develop two piezoelectric textile devices formed from different weft knitted fabric rapports (Jersey and Pique) to be applied in the renewable energy’s (RE) area.

Design/methodology/approach
Two different weft knitted rapports were produced with polyester (PES). The device developed has five layers: a central of poly(vinylidene fluoride) (PVDF) nonwoven, involved by two insulating layers of PES knitted fabric; and two conductive external layers, made of polypyrrole-coated PES knitted fabric. The piezoelectric textile devices were joined by sewing the five layers of the device.

Findings
The FTIR technique confirmed the β-phase in the PVDF nonwoven. This study produced and tested two different textiles devices with piezoelectric behavior, confirmed by the correlated pattern of voltage and tensile stress difference curves, showing the potential application in RE’s and sustainable energies field as smart textiles, such as devices incorporated in garments in the areas of high movement (elbow, knee, foot, fingers and hands, among others), and as an energy generator device

Originality/value
Textile materials with piezoelectric properties promise to advance RE’s developments due to their high material flexibility and sensitivity to the electrical response. The knitted fabric technology presents flexibility due to its construction process. Comparative studies analyzing the electrical response between knitted and woven fabrics have already been realized. However, there is a gap in terms of research scientific research regarding the comparison of the piezoelectric effect in a material that presents different knitted fabric rapports.
Evaluation of solanum tuberosum potato peel waste for use as an eco-friendly antibacterial finish for cotton fabrics
Authors: Mpofu Nonsikelelo Sheron , Mwasiagi Josphat Igadwa , Mecha Cleophas Achisa , Nganyi Eric Oyondi 
Published: October 2023

Purpose
This study aims to investigate the potential use of potato peel extracts as antibacterial finishes for cotton fabrics against Staphylococcus aureus and Escherichia coli. Potato peels are abundant as waste and provide a natural, cheaper and sustainable alternative means of preventing the spread of bacterial infections on cotton fabric.

Design/methodology/approach
This research included the characterization of potato peel extracts, application of the extract onto cotton fabric and efficacy testing of the treated cotton fabric against bacteria. Phytochemical screening, agar well diffusion antibacterial test, minimum inhibitory concentration and Fourier transform infrared (FTIR) tests were used to characterize the extract. Antibacterial efficacy of the treated fabric was determined qualitatively using the disc diffusion assay and quantitatively using the bacteria reduction test.

Findings
Phytochemical screening confirmed the presence of several secondary metabolites including phenols and flavonoids. Antibacterial tests revealed a positive response in Escherichia coli and Staphylococcus aureus with a zone of inhibition of 6.50 mm and 5.60 mm, respectively. Additional peaks on the FTIR spectroscopy confirmed the presence of potato peel extract on the treated cotton fabric. The treated cotton fabrics showed efficacy against Staphylococcus aureus and Escherichia coli up to 20 washes.

Originality/value
This study introduced the application of potato peel extracts onto cotton fabrics and assessment of the antibacterial properties before and after washing. Results of this study suggest that potato peel extracts can be used as an organic eco-friendly antibacterial finish for cotton fabrics.
Positive family connections: co-producing a virtual group programme for family carers of children with learning disabilities or who are autistic
Authors: Griffin Joanna , Austin Debbie , Lynham John , Hafidh Rasha , Boxill Natasha , Sutherland Daniel , Flynn Samantha , Hastings Richard P. 
Published: October 2023

Purpose
This paper aims to outline the process of developing a new co-produced virtual group support programme called Positive Family Connections (PFC) aimed at family carers of children with a learning disability, or who are autistic, aged between 8 and 13 years.

Design/methodology/approach
Development process: family carers were recruited to develop PFC prior to a feasibility randomised controlled trial being conducted (not reported in this paper). The programme was positively oriented and family systems-focused. PFC was developed by family carers, along with the research team, and designed to be delivered by family carer facilitators. The development process included several meetings to design the format and content of the programme. An initial pilot was then delivered and further amendments made to the programme in response to the pilot participants’ feedback.

Findings
The programme: the co-produced PFC programme involved attending six weekly sessions on Zoom; each 2-h session focused on different themes (e.g. communication and activities).

Research limitations/implications
Reflections on the co-production process: key ingredients of co-production included ensuring clarity on roles, positive communication and understanding of the family carers’ situation and utilising the varied skills family carers can bring to research and practise.

Originality/value
This is the first family systems-focused programme that the authors know of, that has been co-produced with family carers and solely delivered virtually by trained family carer facilitators from the outset.