bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-molecule analysis of intracellular insulin granule behavior and its application to analyzing cytoskeletal dependence and pathophysiological implications
Authors: Hatakeyama Hiroyasu , Oshima Tomomi , Ono Shinichiro , Morimoto Yuichi , Takahashi Noriko 
Published: December 2023

Introduction: Mobilization of intracellular insulin granules to the plasma membrane plays a crucial role in regulating insulin secretion. However, the regulatory mechanisms of this mobilization process have been poorly understood due to technical limitations. In this study, we propose a convenient approach for assessing intracellular insulin granule behavior based on single-molecule analysis of insulin granule membrane proteins labeled with Quantum dot fluorescent nanocrystals.Methods: This approach allows us to analyze intracellular insulin granule movement with subpixel accuracy at 33 fps. We tracked two insulin granule membrane proteins, phogrin and zinc transporter 8, fused to HaloTag in rat insulinoma INS-1 cells and, by evaluating the tracks with mean-square displacement, demonstrated the characteristic behavior of insulin granules.Results and discussion: Pharmacological perturbations of microtubules and F-actin affected insulin granule behavior on distinct modalities. Specifically, microtubule dynamics and F-actin positively and negatively regulate insulin granule behavior, respectively, presumably by modulating each different behavioral mode. Furthermore, we observed impaired insulin granule behavior and cytoskeletal architecture under chronic treatment of high concentrations of glucose and palmitate. Our approach provides detailed information regarding intracellular insulin granule mobilization and its pathophysiological implications. This study sheds new light on the regulatory mechanisms of intracellular insulin granule mobilization and has important implications for understanding the pathogenesis of diabetes.
Ultra-high static magnetic field induces a change in the spectrum but not frequency of DNA spontaneous mutations in Arabidopsis thaliana
Authors: Xu Xiang , Chen Mengjiao , Chen Tianli , Ni Xinda , Fang Zhicai , Fang Yanwen , Zhang Lei , Zhang Xin , Huang Jirong 
Published: December 2023

Biological effects of magnetic fields have been extensively studied in plants, microorganisms and animals, and applications of magnetic fields in regulation of plant growth and phytoprotection is a promising field in sustainable agriculture. However, the effect of magnetic fields especially ultra-high static magnetic field (UHSMF) on genomic stability is largely unclear. Here, we investigated the mutagenicity of 24.5, 30.5 and 33.0 T UHSMFs with the gradient of 150, 95 and 0 T/m, respectively, via whole genome sequencing. Our results showed that 1 h exposure of Arabidopsis dried seeds to UHSMFs has no significant effect on the average rate of DNA mutations including single nucleotide variations and InDels (insertions and deletions) in comparison with the control, but 33.0 T and 24.5 T treatments lead to a significant change in the rate of nucleotide transitions and InDels longer than 3 bp, respectively, suggesting that both strength and gradient of UHSMF impact molecular spectrum of DNA mutations. We also found that the decreased transition rate in UHSMF groups is correlated with the upstream flanking sequences of G and C mutation sites. Furthermore, the germination rate of seeds exposed to 24.5 T SMF with -150 T/m gradient showed a significant decrease at 24 hours after sowing. Overall, our data lay a basis for precisely assessing the potential risk of UHSMF on DNA stability, and for elucidating molecular mechanism underlying gradient SMF-regulated biological processes in the future.
Lessons learned from the COVID-19 response in Sri Lankan hospitals: an interview of frontline healthcare professionals
Authors: Munasinghe Nimali Lakmini , O'Reilly Gerard , Cameron Peter 
Published: December 2023

IntroductionThe COVID-19 pandemic revealed the lack of preparedness in health systems, even in developed countries. Studies published on COVID-19 management experiences in developing countries, including Sri Lanka, are significantly low. Therefore, lessons learned from pandemic management would be immensely helpful in improving health systems for future disaster situations. This study aimed to identify enablers and barriers to COVID-19 management in Sri Lankan hospitals through healthcare workers’ perceptions.MethodsFrontline doctors and nurses from different levels of public hospitals were interviewed online. Both inductive and deductive coding and thematic analysis were performed on the transcribed data.Result and discussionThis study identified four themes under enablers: preparing for surge, teamwork, helping hands and less hospital-acquired infections. Seven themes were identified as barriers: lack of information sharing, lack of testing facilities, issues with emergency equipment, substandard donations, overwhelmed morgues, funding issues and psychological impact. These preparedness gaps were more prominent in smaller hospitals compared with larger hospitals. Recommendations were provided based on the identified gaps.ConclusionThe insights from this study will allow health administrators and policymakers to build upon their hospital’s resources and capabilities. These findings may be used to provide sustainable solutions, strengthening the resilience of the local Sri Lankan health system as well as the health systems of other countries.
L'audace du rail : les trains blindés du Sud-Annam.
Authors: Neviaski Alexis 
Published: December 2023

Railways, though extremely vulnerable, are essential to the flow of economic goods and military supplies. It thus fell to soldiers to provide for their defence. Little known, but audacious, armoured trains proved to be an efficacious means to do this, evidenced by their actions in the war in Indochina and particularly in Southern Annam. The rail network was of crucial importance in this territory, for it formed the sole mode of communications. Dispositions were made to ensure the network’s integrity - but they lacked the essential tool to prevent the rail link being interrupted : an armoured train.
The survival of these mobile fortresses was due to several trade-offs between their armour protection, speed and firepower. Placed at the service of the railway system, this combat asset undertook missions of all kinds : on the track (repair and maintenance), supporting outposts (in defence and resupply), and with other trains (escort and support duties). Participating in operations planned by the military staffs, the armoured trains were truly effective in the struggle against the guerrillas, generating a climate of insecurity around the railway line. Constantly in action, by day and night, they surprised and destroyed the Viet-minh through a combination of flexible manoeuvre by the troops they carried on board and the firepower of the train’s guns.
Effective in a distant conflict, long-ago, are armoured trains purely a weapons-system of the past ? Assuredly not, for several of them made an appearance in the recent conflict in the former-Yugoslavia. Could armoured trains be useful in Western Europe, where they are currently non-existent ? Not right now -but this is an asset worth reconsidering as a counter to future terrorist attack against the railways.
СЕРЦЕ ВІДДАЮ ДІТЯМ – ПОГЛЯД В. СУХОМЛИНСЬКОГО НА ВЧИТЕЛІВ ТА ЙОГО СУЧАСНЕ ЗНАЧЕННЯ
Authors: Пен Тен , Су Сяо 
Published: December 2023

Теоретичною основою всебічної гармонійної педагогічної концепції В. Сухомлинського є гуманістична філософія та соціально-історична культурна теорія, започаткована у свій час Л. Виготським. Педагогічні ідеї В. Сухомлинського як вчителя та директора є кристалізацією його довготривалої педагогічної практики, яка не тільки вплинула на освіту того часу, а й мала великий вплив в усьому світі. В. Сухомлинський вважав, що хорошому вчителю недостатньо володіти теоретичною грамотністю – він повинен також мати високий професіоналізм і прагматичний дослідницький дух, вірити у виховний потенціал «любові» та надавати значення самоосвіті.
Carcinoma Escamoso de Cérvix en una Paciente de 21 Años: Reporte de Caso
Authors: Vergara Cárcamo Robert Fernando , Vergara Ealo Sebastián , Salas - Navarro Claudia Patricia , Duque - Uribe Camilo , Salas – Navarro Jennifer 
Published: December 2023

Introducción: La guía de práctica clínica del ministerio de salud de Colombia, para la detección y manejo de lesiones precancerosas del cuello uterino, recomienda iniciar tamizaje para la prevención del cáncer de cérvix a partir de los 25 años. Métodos: Se presenta el caso de una paciente de 21 años, quien acudió al servicio de urgencias por cuadro clínico caracterizado por dolor abdominal asociado a sangrado vaginal. Mediante ecografía pélvica transvaginal se visualizó pólipo cervical, por lo que la paciente fue sometida a legrado. Resultados: La lesión fue extraída y enviada para análisis anatomopatológico y caracterización inmunohistoquímica. El resultado de patología concluyó que paciente cursaba con carcinoma escamoso del cérvix. Conclusión: No hay evidencia que indique que es acertado disminuir la edad para el inicio del cribado, a pesar de que, en ocasiones, se producen neoplasias en mujeres menores de 25 años. La forma más eficaz de disminuir la incidencia de lesiones malignas cervicales es utilizando una estrategia conjunta basada en tamizaje y vacunación contra el virus del papiloma humano.
Revisiting ϵ Eridani with NEID: Identifying New Activity-sensitive Lines in a Young K Dwarf Star
Authors: Jiang Sarah , Roy Arpita , Halverson Samuel , Bender Chad F. , Selgas Carlos , Otor O. Justin , Mahadevan Suvrath , Stefánsson Guđmundur , Terrien Ryan C. , Schwab Christian 
Published: December 2023

Abstract
Recent improvements in the sensitivity and precision of the radial velocity (RV) method for exoplanets have brought it close, but not quite to, the threshold (∼10 cm s−1) required to detect Earth-mass and other potentially habitable planets around Sun-like stars. Stellar activity-driven noise in RV measurements remains a significant hurdle to achieving this goal. While various efforts have been made to disentangle this noise from real planetary signals, a greater understanding of the relationship between spectra and stellar activity is crucial to informing stellar activity mitigation. We use a partially automated method to analyze spectral lines in a set of observations of the young, active star ϵ Eridani from the high-precision spectrograph NEID, correlate their features (depth, FWHM, and integrated flux) with known activity indicators, and filter and curate for well-defined lines whose shape changes are sensitive to certain types of stellar activity. We then present a list of nine lines correlated with the S-index in all three line features, including four newly identified activity-sensitive lines, as well as additional lines correlated with the S-index in at least one feature, and discuss the possible implications of the behavior observed in these lines. Our line lists represent a step forward in the empirical understanding of the complex relationships between stellar activity and spectra and illustrate the importance of studying the time evolution of line morphologies with stabilized spectrographs in the overall effort to mitigate activity in the search for small, potentially Earth-like exoplanets.
A Spatially Resolved X-Ray Polarization Map of the Vela Pulsar Wind Nebula
Authors: Liu Kuan , Xie Fei , Liu Yi-han , Ng Chi-Yung , Bucciantini Niccolò , Romani Roger W. , Weisskopf Martin C. , Costa Enrico , Di Marco Alessandro , La Monaca Fabio , Muleri Fabio , Soffitta Paolo , Deng Wei , Meng Yu , Liang En-wei 
Published: December 2023

Abstract
In this paper, we present a full spatially resolved polarization map for the Vela pulsar wind nebula (PWN) observed by IXPE. By employing effective background discrimination techniques, our results show a remarkably high degree of local polarization in the outskirt region, exceeding 60% (55%) with a probability of 95% (99%), which approaches the upper limit predicted by the synchrotron emission mechanism. The high degree of polarization suggests that the turbulent magnetic energy is at most 33% of the ordered one. In addition, the X-ray polarization map exhibits a toroidal magnetic field pattern that is consistent with the field revealed by radio observations across the entire nebula. This consistency reveals that the observed X-ray and radio emissions are radiated by electrons from the same magnetic field. Different from the Crab PWN, the consistency observed in the Vela PWN may be attributed to the interaction between the reverse shock of the supernova blast wave and the PWN, which leads to a displacement between the synchrotron-cooled nebula and the fresh nebula close to the pulsar. These findings deepen our understanding of the structure and evolution of the Vela PWN and the magnetohydrodynamic interaction in PWNe.
Creating a multi-linked dynamic dataset: a case study of plant genera named for women
Authors: von Mering Sabine , Gardiner Lauren , Knapp Sandra , Lindon Heather , Leachman Siobhan , Ulloa Ulloa Carmen , Vincent Sarah , Vorontsova Maria 
Published: December 2023

A discussion on social media led to the formation of a multidisciplinary group working on this project to highlight women’s contributions to science. The role of marginalised groups in science has been a topic of much discussion, but data on these contributions are largely lacking. Our motivation for the development of this dataset was not only to highlight names of plant genera that honour women, but to enrich this information with data that would allow the names, roles and lives of these women to be shared more widely with others, both researchers and data sources like Wikidata. Amplification of the contributions of women to botany through multiple means will enable the community to better recognise and celebrate the role of this particular marginalised group in the history and development of science.The innovative approach of our study resulted in a dataset that is dynamic, expansive and widely shared. We have published a static dataset with this paper and have also created a dynamic dataset by linking flowering plant genera and the women in whose honour those genera were named in Wikidata. This concurrent addition of the data to Wikidata, a linked open data repository, enabled it to be enriched, queried and proactively shared during the whole process of dataset creation and into the future. This innovative workflow allowed wide, open participation throughout the research process. The methodology and workflows applied can be used to create future datasets celebrating and amplifying the contributions of marginalised groups in science.
Réflexions sur l’accompagnement des soignants exerçant en psychiatrie pendant la crise sanitaire du Covid-19
Authors: Tsagkari Vasiliki 
Published: December 2023

La pandémie du Covid-19 a déclenché une situation de crise sanitaire imposant au personnel soignant des hôpitaux une nouvelle organisation de travail. Sans être au front de la pandémie, l’hôpital psychiatrique s’organise néanmoins afin de pouvoir accueillir des patients psychiatriques touchés par le Covid-19. Quel est l’impact psychique de cette crise pour les soignants ? Cet article s’intéresse à l’accompagnement psychologique proposé au personnel de l’hôpital psychiatrique pendant la première vague de cette crise. Les missions du psychologue clinicien auprès du personnel y seront discutées ainsi que les changements dans l’organisation du travail en lien avec la pandémie. Les questions de la relation thérapeutique, du cadre et de ses limitations y seront également abordées.
Crise du discours : solitudes et invention subjective
Authors: Tible Jessica 
Published: December 2023

L’émergence de la Covid-19 et les modes de régulation nécessaires de la pandémie ont ouvert une réflexion sur le modèle sociétal contemporain et la possibilité du « jour d’après » en rupture avec le système capitalisme et son économie libérale. Dès lors, nous proposons une analyse du traitement « biopolitique » de la pandémie afin de dégager les coordonnées d’un nouveau paradigme sociétal : celui d’un régime de domination par les « lathouses » numériques réhabilitées d’utilité sociale. Un mode de gouvernance des vivants qui se présente comme solution à l’errance subjective que produit le « discours du capitaliste », tel qu’il fut formalisé de façon inédite par Jacques Lacan en 1972. À cet égard, nous ferons valoir ce que permet, au niveau du singulier, une pratique analytique sous transfert et ses effets de lien social.
Le symptôme dans la peau. Écrire la scarification en clinique
Authors: Roose-Beauprez Olivia , Reniers Dominique , Guillén Julio 
Published: December 2023

À partir de l’étude d’un cas clinique de notre pratique, nous proposons d’interroger la place des scarifications aujourd’hui, notamment dans leur valeur symptomatique. Nous nous intéresserons tout particulièrement à l’action de se scarifier en ce qu’elle peut être entendue à partir des référentiels psychiatriques et psychanalytiques. Ce cheminement nous amènera à introduire l’acte d’énonciation dans lequel le symptôme prend statut et à nous arrêter sur la position du clinicien dans la dynamique transférentielle où certains éléments que nous avons présentés peuvent trouver leurs coordonnées de symptôme.
Du signifiant formel à la narration : l’apport du concept d’enveloppe psychique dans la clinique du traumatisme en situation interculturelle
Authors: Landbeck Alicia , Mellier Denis , Laurent Alexandra 
Published: December 2023

La clinique humanitaire conjugue régulièrement traumatismes psychiques, carences précoces et environnement marqué par la précarité. Sur ces terrains, l’accompagnement thérapeutique est souvent repensé, au regard de la culture et des spécificités de la clinique. Dans le cadre d’une recherche menée au Cambodge, auprès d’enfants victimes de sévices, un dispositif pensé au regard du concept d’enveloppe psychique a été mis en place. Durant un an, dix enfants ont été rencontrés. Dans le cadre de cet article nous présentons comment le concept d’enveloppe psychique a servi d’étayage théorique au thérapeute pour penser les infimes mouvements psychiques sous le prisme de la sensorialité, des mouvements du corps et de la construction-reconstruction des enveloppes psychiques primaires. Nous présentons la situation clinique de Poeu et les différentes étapes de sa prise en charge. Durant les rencontres, le thérapeute a été confronté à des défenses adhésives et à un espace psychique bidimensionnel. L’appui sur le concept de signifiant formel comme origine de la constitution des enveloppes et du Moi-peau a constitué une aide au thérapeute et à l’interprète pour ne pas céder à leur désir de soin et se saisir des spécificités cliniques de la situation : les aspects sensoriels primaires, la remise en mouvement du corps ainsi que l’expression corporelle dans la mise en sens partageable de contenus psychiques. La verbalisation sous forme de narration des mouvements et signifiants formels a permis la remise en mouvement et l’élaboration psychique. Nous proposons ainsi le terme de « narrativité formelle » pour envisager la transformation des enveloppes psychiques dans un dispositif qui a rendu possible la rencontre des thérapeutes avec la souffrance des sujets.
« Travailler au bonheur de [son] compagnon » : Marie-Thérèse Gravier et Émile Ollivier (décembre 1868-août 1870)
Authors: Berthiaud Emmanuelle 
Published: December 2023

En septembre 1869, Marie-Thérèse Gravier épouse Émile Ollivier, un homme politique en vue qui devient chef du gouvernement libéral de Napoléon III, de janvier 1870 jusqu’à leur exil en août 1870. Pendant ces quelques mois, elle fait le rude apprentissage de la vie d’épouse de ministre dans un gouvernement très exposé. Ses écrits privés révèlent le travail largement invisible et peu valorisé que cette fonction implique, la nécessité de repenser le rôle politique des femmes et la frontière habituellement établie entre sphère privée et sphère publique.
Pensées Imperceptibles in Arnauld and Nicole
Authors: Stencil Eric , Walsh Julie 
Published: December 2023

Dans cet article, nous nous concentrons sur les réflexions qu’Antoine Arnauld et Pierre Nicole consacrent au concept de « pensées imperceptibles », en soulignant à quel point ces réflexions sont liées à leur doctrine de la grâce et de la condition postlapsaire. Nous montrons à cet égard combien les débats métaphysiques de la première modernité sont inséparables d’un certain nombre d’engagements éthiques et politiques. En particulier, les préjugés de nos penseurs sur les capacités intellectuelles des non-Européens ne sont pas extérieurs à ce débat mais en sont, au contraire, le moteur.
Se tromper de bonne foi
Authors: Ferraro Angela 
Published: December 2023

Cet article se propose de montrer comment, en se penchant sur la question épineuse des habitudes de l’âme, la pensée de Malebranche a intégré et renouvelé le débat sur la thématique, chère aux moralistes du Grand Siècle, de l’insondabilité du cœur humain. Dans ce cadre, il s’agira surtout de mettre au jour les rapports qui se dessinent, à l’intérieur du corpus malebranchiste, entre une série de couples conceptuels : logique et morale, sensation et sentiment intérieur, conscience psychologique et conscience morale, péché et erreur, reproches de la raison et remords. Des éléments de réponse seront ainsi apportés aux interrogations de fond qui persistent sur la nature et l’orientation de la philosophie morale de l’oratorien.
Simulation Modeling to Assess Line Transect Distance Sampling Under a Range of Translocation Scenarios
Authors: Jones Max D. , Smith Lora L. , Richardson Katherine Gentry , DeSha J. Nicole , Castellón Traci , Hipes Dan , Kalfin Alex , Halstead Neal T. , Hunter Elizabeth A. 
Published: December 2023

Abstract
The accuracy of posttranslocation population monitoring methods is critical to assessing long-term success in translocation programs. Translocation can produce unique challenges to monitoring efforts; therefore, it is important to understand the flexibility and robustness of commonly used monitoring methods. In Florida, USA, thousands of gopher tortoises Gopherus polyphemus have been, and continue to be, translocated from development sites to permitted recipient sites. These recipient sites create a broad range of potential monitoring scenarios due to variability in soft-release strategies, habitat conditions, and population demographics. Line transect distance sampling is an effective method for monitoring natural tortoise populations, but it is currently untested for translocated populations. We therefore produced 3,024 individual-based, spatially explicit scenarios of translocated tortoise populations that differed in recipient site and tortoise population properties, based on real-world examples, literature review, and expert opinion. We virtually sampled simulated tortoise populations by using line transect distance sampling methods and built a Bayesian hierarchical model to estimate the population density for each simulation, which incorporated individual-level covariates (i.e., burrow width and burrow occupancy). Line transect distance sampling was largely appropriate for the conditions that typify gopher tortoise recipient sites, particularly when detection probability on the transect lines was greater than or equal to 0.85. Designing the layout of transects relative to the orientation of soft-release pens, to avoid possible sampling biases that lead to extreme outliers in estimates of tortoise densities, resulted in more accurate population estimates. We also suggest that use of individual-level covariates, applied using a Bayesian framework as demonstrated in our study, may improve the applicability of line transect distance sampling surveys in a variety of contexts and that simulation can be a powerful tool for assessing survey design in complex sampling situations.