bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fe3O4‐nanoparticle‐functionalized 3D‐printed injectable microgel
Authors: Wei Xinlin , Zheng Taijing , Zhu Shunyao , Liao Xueyuan , Zhou Nazi , Wang Yu , Xu Yue , Xiong Meimei , Liu Rui , Mao Jian , Tao Jie 
Published: November 2023

AbstractMagnetic Fe3O4 nanoparticles have attached attention in bone tissue engineering because of their superior magnetism and great biocompatibility. However, some disadvantages such as the potential risk of agglomeration impair their applications. Here, we proposed a hybrid magnetic nanocomposite microgel by the integration of Fe3O4 nanoparticles and digital lighting processing (DLP) three‐dimensional (3D) printing technology. The 3D‐printed microgels could be precisely customized by printing the mixture of gelatin methacryloyl (GelMA) solution and polydopamine‐coated Fe3O4 nanoparticles, in which polydopamine decoration improved the hydrophilicity and distribution of the incorporated Fe3O4. The degradable microgels could be injected through a 22‐G needle while retaining their original shape after injection. Interestingly, the addition of Fe3O4 nanoparticles into GelMA solution displayed improved printing accuracy. Moreover, these magnetic microgels were biocompatible in vitro and in vivo. After induction within osteogenic medium, addition of nanoparticles upregulated the osteogenic gene expression of rat bone mesenchymal stem cells (BMSCs). In a word, this work provides a magnetic microplatform, which shows great potential in bone tissue engineering.
The effects of morin and methotrexate on pentose phosphate pathway enzymes and GR/GST/TrxR enzyme activities: An in vivo and in silico study
Authors: Caglayan Cuneyt , Temel Yusuf , Türkeş Cüneyt , Ayna Adnan , Ece Abdulilah , Beydemir Şükrü 
Published: November 2023

AbstractIn this study, the mechanisms by which the enzymes glucose‐6‐phosphate dehydrogenase (G6PD), 6‐phosphogluconate dehydrogenase (6PGD), glutathione reductase (GR), glutathione‐S‐transferase (GST), and thioredoxin reductase (TrxR) are inhibited by methotrexate (MTX) were investigated, as well as whether the antioxidant morin can mitigate or prevent these adverse effects in vivo and in silico. For 10 days, rats received oral doses of morin (50 and 100 mg/kg body weight). On the fifth day, a single intraperitoneal injection of MTX (20 mg/kg body weight) was administered to generate toxicity. Decreased activities of G6PD, 6PGD, GR, GST, and TrxR were associated with MTX‐related toxicity while morin treatment increased the activity of the enzymes. The docking analysis indicated that H‐bonds, pi–pi stacking, and pi–cation interactions were the dominant interactions in these enzyme‐binding pockets. Furthermore, the docked poses of morin and MTX against GST were subjected to molecular dynamic simulations for 200 ns, to assess the stability of both complexes and also to predict key amino acid residues in the binding pockets throughout the simulation. The results of this study suggest that morin may be a viable means of alleviating the enzyme activities of important regulatory enzymes against MTX‐induced toxicity.
Dehydroacetic acid a privileged medicinal scaffold: A concise review
Authors: Grover Anshul , Kumar Aman , Tittal Ram Kumar , Lal Kashmiri 
Published: November 2023

AbstractFrom the last decade, research on dehydroacetic acid (DHA) and its derivatives has increased immensely due to its significant role in various fields, including medicine, cosmetics, food industry, and so on. In the medicinal area, DHA plays an essential role in developing novel action‐based drugs, which are helpful for treating various diseases. Besides its plethora of biological applications, its chelating ability offers the easiest synthetic route for synthesizing more active metal complexes. DHA derivatives along with their metal complexes show a number of biological activities and also exhibit various interactions with multiple biological targets. This article summarizes recent medicinal applications (2000‐onwards) of DHA‐based compounds and their analogs, along with their structure–activity relationship (SAR) analysis. Their interactions with different target enzymes are also discussed. This information derived from SAR analysis would be helpful for medicinal chemists working on the development of drugs based on heterocyclic frameworks, particularly those based on the DHA scaffold.
Development of emotion comprehension in children with autism spectrum disorder and Williams syndrome
Authors: Ikeda Ayaka , Hakuno Yoko , Asada Kosuke , Ikeda Takahiro , Yamagata Takanori , Hirai Masahiro 
Published: November 2023

AbstractAlthough research has shed light on the development of emotion comprehension in typically developing children, little is known about emotion comprehension in children who are developing atypically. Thus, this study examined the developmental trajectory of emotion understanding in non‐clinical (NC) children and children with autism spectrum disorder (ASD) and Williams syndrome (WS) using a Test of Emotion Comprehension. In the test, we measured children's understanding of (I) recognition of emotions based on facial expressions, (II) external causes of emotions, (III) desire‐based emotions, (IV) belief‐based emotions, (V) the influence of a reminder on a present emotional state, (VI) regulating an experienced emotion, (VII) hiding an emotional state, (VIII) mixed emotions, and (IX) moral emotions. A Bayesian modeling approach was applied to compare the developmental trajectories of emotion understanding across the syndrome groups. The results revealed that NC children and children with WS followed significantly different developmental trajectories in specific aspects of emotion understanding, while children with ASD followed a very similar path to NC children. Children with ASD and NC children gradually developed an understanding of each component of emotion comprehension as they matured. However, the understanding of some components, such as desire‐based emotions, hiding an emotional state, and moral emotions, in children with WS was affected by their Autism Spectrum Quotient scores. This is one of the first cross‐syndrome studies to assess the development of emotion comprehension in children with ASD and WS, providing important insights for understanding the nature of disability and advancing the development of intervention programs.
In‐situ Polymerized Single Lithium‐ion Conducting Binder as an Integrated Strategy for High Voltage LNMO Electrodes
Authors: Olmedo‐González Jorge , Guzmán‐González Gregorio , Manzo‐Robledo Arturo , Ramírez‐Rosales Daniel , Arellano‐Ahumada Stephany N. , Vera‐Ramírez Marco A. , González Ignacio , de Guadalupe González‐Huerta Rosa , Ramos‐Sanchez Guadalupe 
Published: November 2023

AbstractHigh voltage spinel LiNi0.5 Mn1.5O4 (LNMO) is a promising material for next generation lithium‐Ion batteries. However, its reactivity near 5 V possess stability and cycling challenges. In this study, a novel integrated approach is employed using a single lithium‐ion conducting polymer binder (SLICPB) to prevent interactions with reactive anions and create a protective layer against electrolyte decomposition. The proposed SLICPB in‐situ polymerization in the LNMO electrodes simplifies the preparation process, reducing costs. SLICPB properties effectively decrease polarization by concentration. For instance, at a discharge capacity of 68 mAh g−1, the voltage hysteresis difference is 0.31 V, enabling higher capacity at 1 C (a 38 % increase) compared to traditional binder electrodes. Notably, this integrated strategy completely replaces the traditional binder without any need for additives, thus avoiding any extra weight in the electrode preparation. Furthermore, SLICPB properties successfully decreasereactivity and diminish the leaching of Mn2+, as evaluated through differential electrochemical mass spectrometry and electron paramagnetic resonance, respectively.
Performance evaluation of portable time synchronization method using eBPF
Authors: Kobori Kazuto , Lee Chunghan , Hirotsu Toshio 
Published: November 2023

SummaryThe Precision Time Protocol (PTP) is a widely used protocol for high‐precision time synchronization, but it requires hardware or driver support for network interface card. Therefore, we propose a portable time synchronization method that is independent of them. Our method uses eBPF to synchronize time by recording timestamps of PTP packets in the kernel. eBPF is provided by the kernel and is backward‐compatible, making it independent of hardware or other software. To demonstrate the portability and high‐precision time synchronization capabilities of our method, we compared the time synchronization precision in physical environment and virtual machine. We also modified server settings that could affect time synchronization precision, and clarified the effects of these changes. The experiments showed that our method can achieve the similar level of time synchronization precision as conventional methods while remaining portable and independent of other components.
Multifunctional Block Copolymers, Acting as Recycling Aids, by Atom Transfer Radical Polymerization
Authors: Szewczyk‐Łagodzińska Matylda , Oleksiuk Dawid , Kowalczyk Sebastian , Czajka Anna , Dużyńska Anna , Łapińska Anna , Ryszkowska Joanna , Dziewit Piotr , Janiszewski Jacek , Plichta Andrzej 
Published: November 2023

AbstractBlock copolymers utilizing oligomeric poly(pentylene‐co‐hexylene carbonate)diol modified with 2,4‐diisocyanatotoluene and further with 2‐bromo‐N‐(3‐hydroxypropyl)‐2‐methylpropanamide were synthesized and utilized as Activators ReGenerated by Electron Transfer Atom Transfer Radical Polymerization macroinitiators to obtain a first generation of multifunctional recycling additives with poly(glycidyl methacrylate‐co‐butyl methacrylate‐co‐methyl methacrylate) side chains, which could act as chain extenders. Then, chosen additive was reacted with a radical scavenger, 3,5‐ditertbutyl‐4‐hydroxybenzoic acid (DHBA), to obtain a second generation of reactive additives. Those copolymers had different numbers of epoxy groups per polymer chain, and different number of epoxides opened with DHBA, hence showed a range of properties, and were utilized as reactive modifiers for polylactide (PLA) extrusion melting. The first‐generation modifiers caused an increase in PLA's blends relative melt viscosity, stabilized material properties, and enhanced impact strength, while the second‐generation modifiers with more than 8 % of epoxide ring opened showed worse properties. However, they managed to suppress the UV degradation of PLA blend plates.
Bop1 is required to establish precursor domains of craniofacial tissues
Authors: Keer Stephanie , Neilson Karen M. , Cousin Helene , Majumdar Himani D. , Alfandari Dominique , Klein Steven L. , Moody Sally A. 
Published: November 2023

SummaryBop1 can promote cell proliferation and is a component of the Pes1‐Bop1‐WDR12 (PeBoW) complex that regulates ribosomal RNA processing and biogenesis. In embryos, however, bop1 mRNA is highly enriched in the neural plate, cranial neural crest and placodes, and potentially may interact with Six1, which also is expressed in these tissues. Recent work demonstrated that during development, Bop1 is required for establishing the size of the tadpole brain, retina and cranial cartilages, as well as controlling neural tissue gene expression levels. Herein, we extend this work by assessing the effects of Bop1 knockdown at neural plate and larval stages. Loss of Bop1 expanded neural plate gene expression domains (sox2, sox11, irx1) and reduced neural crest (foxd3, sox9), placode (six1, sox11, irx1, sox9) and epidermal (dlx5) expression domains. At larval stages, Bop1 knockdown reduced the expression of several otic vesicle genes (six1, pax2, irx1, sox9, dlx5, otx2, tbx1) and branchial arch genes that are required for chondrogenesis (sox9, tbx1, dlx5). The latter was not the result of impaired neural crest migration. Together these observations indicate that Bop1 is a multifunctional protein that in addition to its well‐known role in ribosomal biogenesis functions during early development to establish the craniofacial precursor domains.
Endemic fish calling: Acoustics and reproductive behaviour of the Neretva dwarf goby Orsinigobius croaticus
Authors: Horvatić Sven , Parmentier Eric , Malavasi Stefano , Amorim Maria P. Clara , Fonseca Paulo J. , Zanella Davor 
Published: November 2023

AbstractThe Neretva dwarf goby Orsinigobius croaticus (Gobiiformes, Gobionellidae) is an endemic fish native to the freshwaters of the Adriatic Basin in Croatia and Bosnia and Herzegovina, a Mediterranean Biodiversity Hotspot. Due to its limited distribution range, specific karst habitat and endangered status, laboratory studies on reproductive biology are scarce but crucial. Herein, we investigated the sound production and acoustic behaviour of the endangered O. croaticus during reproductive intersexual laboratory encounters, utilising an interdisciplinary approach. We also performed dissections and micro‐computed tomography (μCT) scanning of the pectoral girdle to explore its potential involvement in sound production. Finally, comparative acoustic analysis was conducted on sounds produced by previously recorded soniferous sand gobies to investigate whether acoustic features are species‐specific. The endemic O. croaticus is a soniferous species. Males of this species emit pulsatile sounds composed of a variable number of short (~15 ms) consecutive pulses when interacting with females, usually during the pre‐spawning phase in the nest, but also during courtship outside the nest. Pulsatile sounds were low‐frequency and short pulse trains (~140 Hz, <1000 ms). Male visual behaviour rate was higher when co‐occurring with sounds and females entered the male's nest significantly more frequently when sounds were present. Characteristic body movements accompanied male sound production, such as head thrust and fin spreading. Furthermore, μCT scans and dissections suggest that O. croaticus shares certain anatomical similarities of the pectoral girdle (i.e. osseous elements and arrangement of levator pectoralis muscles) to previously studied sand gobies that could be involved in sound production. Multivariate comparisons, using sounds produced by eight soniferous European sand gobies, effectively distinguished soniferous (and sympatric) species based on their acoustic properties. However, the discrimination success decreased when temperature‐dependent features (sound duration and pulse repetition rate) were excluded from the analysis. Therefore, we suggest both spectral and temporal features are important for the acoustic differentiation of sand gobies.
Forest and snow rather than food or foe limit the distribution of a generalist mesocarnivore in winter
Authors: Butler Andrew R. , Evans Bryn E. , Mortelliti Alessio , Moll Remington J. 
Published: November 2023

AbstractInvestigating species responses to trophic interactions and abiotic factors is crucial to better understanding their ecology and creating effective management strategies. In carnivore communities, smaller species are often regulated by larger ones via top‐down interference competition. Smaller subordinate carnivores can also be regulated by bottom‐up and abiotic factors, such as the availability of important prey, habitat features, and climatic conditions. However, substantial ambiguity remains regarding the relative roles these complex factors play in shaping subordinate carnivore populations, especially during winter. To investigate this issue, we conducted a large‐scale camera‐trapping study (n = 197 sites distributed across a ~60,000 km2 landscape) using a balanced study design that sampled a gradient of forest disturbance and climatic conditions. We used dynamic occupancy modeling to examine the influences of top‐down (interference competition), bottom‐up (prey and habitat), and abiotic (climate) factors on a widespread, generalist subordinate carnivore, the red fox (Vulpes vulpes), in Maine, USA. Across three winters, we collected 107 red fox and 185 coyote (Canis latrans) daily detections, and 3875 snowshoe hare (Lepus americanus) detections. We found evidence for the top‐down effects of coyotes on red fox detection probability and site colonization. However, contrary to theoretical expectations, the association between coyotes and red foxes was positive rather than negative. Snowshoe hares had a positive association with local extinction by red foxes, which also contrasts with prevailing theory given that snowshoe hares are an important winter prey of red foxes in this ecosystem. The intensity of forest disturbance and the proportion of conifer forest had negative effects on red fox occurrence and detection probability, while snow depth had a strong negative effect on site colonization. Together, these results suggest red foxes are limited more by abiotic and bottom‐up factors related to habitat than by the top‐down interference competition or primary prey availability in winter. Our study supports recent findings that bottom‐up factors may shape carnivore distributions during less productive times of year. Our work also highlights how caution is needed when extrapolating previous results from summer studies to winter, as the role of top‐down and bottom‐up factors may change seasonally.
Visible‐Light‐Induced Bromination of α‐Diazo Compounds with Bromoform
Authors: Zhang Xin , Lin Junyue , Liu Miao‐Chang , Zhou Yun‐Bing , Wu Hua‐Yue 
Published: November 2023

AbstractAn efficient visible‐light‐driven strategy for bromination of α‐diazo compounds is achieved for the construction of α‐bromoesters with bromoform. It is noteworthy that the reaction occurs under sole light irradiation without a photocatalyst or an additive. Several synthetic transformations and gram‐scale reactions demonstrate the synthetic potential of this photochemical reaction.
Forecasting tourist flows in the COVID‐19 era using nonparametric mixed‐frequency VARs
Authors: You Wanhai , Huang Yuming , Lee Chien‐Chiang 
Published: November 2023

AbstractIt is widely recognized that the tourism industry is susceptible to crisis or natural disaster. Although some literature has studied the consequences of the crisis and disaster, there remains a lack of study on the effect of COVID‐19. Against this background, this paper investigates the tourist flow forecasting by adopting an advanced nonparametric mixed‐frequency vector autoregressions model using Bayesian additive regression trees. This is particularly suitable for forecasting the presence of extreme observations, for example, the COVID‐19 pandemic. We investigate tourism demand forecasting using a large number of predictors, including industrial production index, CPI, exchange rate, economic policy uncertainty, Google trends index, and COVID‐19 infection rate. The data used for this study relate to tourist flows in Chinese Hong Kong, Japan, and South Korea. Empirical study demonstrates that this novel model significantly outperforms the traditional mixed‐frequency vector autoregressions model to quarterly tourist flow forecasting. Therefore, this model can significantly enhance tourism forecast accuracy in the face of extreme events. This study contributes to the literature on tourism forecasting and provides policymakers with policy implications.
Application of the cell‐based RT‐qPCR assay (C‐QPA) for potency detection of the novel trivalent rotavirus vaccine in China
Authors: Wang Yunjin , Liu YueYue , Bao Hong , Chen Yueru , Kou Guiying , Wang Mingqiang , Fu Shengfang , Huo Wen , Guan Wenzhu , Cheng Yahui , Zhou Xu , Li Xiongxiong 
Published: November 2023

AbstractBackgroundBecause of the deficiencies of traditional methods in multivalent rotavirus vaccine potency detection, a cell‐based quantitative RT‐qPCR assay (C‐QPA) was established and validated for specificity, precision, and accuracy.MethodsIn order to further validate the robustness of this method in actual titer detection, the linear range and the practical application under different conditions were tested using monovalent and trivalent rotavirus samples and standards.ResultsResults showed that the linear range was 2.0–6.5, 3.9–8.3, and 3.5–8.1 UI (unit of infectivity) for G2, G3, and G4, respectively. Besides, unknown sample with high titer exceeding the linear range can be calculated by dilution. The UIs of serotypes G2, G3, and G4 in monovalent and trivalent rotavirus samples showed a relative deviation ≤4.10%, and the monovalent samples of the same serotype with or without protective agents showed a relative deviation ≤4.28%; the coefficient of variation (CV) of at least 176 tests (548 individual runs) of 3 in vitro‐transcribed RNA standards with certain concentrations was not higher than 6.50%; the results of the trivalent samples tested by more than 149 times in 5 years (467 individual runs) showed the CVs lower than 12.66%; 15 samples detected by one laboratory showed a CV lower than 9.83%, while other three samples tested by two independent laboratories showed a CV lower than 6.90%.ConclusionIn summary, the C‐QPA has good linearity, durability, repeatability, and reproducibility in practical application and has been proved by the authority to be widely used in the production, quality control and release of the recently licensed trivalent vaccine in China.
Henri de Lacaze‐Duthiers and the ascidian hypothesis
Authors: Jessus Catherine , Laudet Vincent 
Published: November 2023

AbstractIn 1830, Cuvier and Geoffroy Saint‐Hilaire confronted each other in a famous debate on the unity of the animal kingdom, which permeated the zoology of the 19th century. From that time, a growing number of naturalists attempted to understand the large‐scale relationships among animals. And among all the questions, that of the origin of vertebrates was one of the most controversial. Analytical methods based on comparative anatomy, embryology and paleontology were developed to identify convincing homologies that would reveal a logical sequence of events for the evolution of an invertebrate into the first vertebrate. Within this context, several theories have clashed on the question of the identity of the ancestor of vertebrates. Among the proposals, a group of rather discrete organisms, the ascidians, played a central role. Because he had discovered an ascidian with a particularly atypical larval development, theMolgula, Henri de Lacaze‐Duthiers, a rigorous and meticulous naturalist, became involved in the ascidian hypothesis. While the visionary mind of Lacaze‐Duthiers led him to establish a particularly innovative methodology and the first marine biology station in Europe, at Roscoff, the tailless tadpole of theMolgulaprevented him from recognizing the ancestor of vertebrates. This old 19th century story echoes the ever‐present questions driving the field of Eco‐Evo‐Devo.