bluuebunny/crossref_metadata_2025_split huggingface dataset:
Declines in ice cover are accompanied by light limitation responses and community change in freshwater diatoms
Authors: Zepernick Brittany N , Chase Emily E , Denison Elizabeth R , Gilbert Naomi E , Truchon Alexander R , Frenken Thijs , Cody William R , Martin Robbie M , Chaffin Justin D , Bullerjahn George S , McKay R Michael L , Wilhelm Steven W 
Published: January 2024

Abstract
The rediscovery of diatom blooms embedded within and beneath the Lake Erie ice cover (2007–2012) ignited interest in psychrophilic adaptations and winter limnology. Subsequent studies determined the vital role ice plays in winter diatom ecophysiology as diatoms partition to the underside of ice, thereby fixing their location within the photic zone. Yet, climate change has led to widespread ice decline across the Great Lakes, with Lake Erie presenting a nearly “ice-free” state in several recent winters. It has been hypothesized that the resultant turbid, isothermal water column induces light limitation amongst winter diatoms and thus serves as a competitive disadvantage. To investigate this hypothesis, we conducted a physiochemical and metatranscriptomic survey that spanned spatial, temporal, and climatic gradients of the winter Lake Erie water column (2019–2020). Our results suggest that ice-free conditions decreased planktonic diatom bloom magnitude and altered diatom community composition. Diatoms increased their expression of various photosynthetic genes and iron transporters, which suggests that the diatoms are attempting to increase their quantity of photosystems and light-harvesting components (a well-defined indicator of light limitation). We identified two gene families which serve to increase diatom fitness in the turbid ice-free water column: proton-pumping rhodopsins (a potential second means of light-driven energy acquisition) and fasciclins (a means to “raft” together to increase buoyancy and co-locate to the surface to optimize light acquisition). With large-scale climatic changes already underway, our observations provide insight into how diatoms respond to the dynamic ice conditions of today and shed light on how they will fare in a climatically altered tomorrow.
Specialized digestive mechanism for an insect-bacterium gut symbiosis
Authors: Lee Junbeom , Jeong Bohyun , Kim Jeongtae , Cho Jae H , Byeon Jin H , Lee Bok L , Kim Jiyeun K 
Published: January 2024

Abstract
In Burkholderia-Riptortus symbiosis, the host bean bug Riptortus pedestris harbors Burkholderia symbionts in its symbiotic organ, M4 midgut, for use as a nutrient source. After occupying M4, excess Burkholderia symbionts are moved to the M4B region, wherein they are effectively digested and absorbed. Previous studies have shown that M4B has strong symbiont-specific antibacterial activity, which is not because of the expression of antimicrobial peptides but rather because of the expression of digestive enzymes, mainly cathepsin L protease. However, in this study, inhibition of cathepsin L activity did not reduce the bactericidal activity of M4B, indicating that there is an unknown digestive mechanism that renders specifically potent bactericidal activity against Burkholderia symbionts. Transmission electron microscopy revealed that the lumen of symbiotic M4B was filled with a fibrillar matter in contrast to the empty lumen of aposymbiotic M4B. Using chromatographic and electrophoretic analyses, we found that the bactericidal substances in M4B existed as high-molecular-weight (HMW) complexes that were resistant to protease degradation. The bactericidal HMW complexes were visualized on non-denaturing gels using protein- and polysaccharide-staining reagents, thereby indicating that the HMW complexes are composed of proteins and polysaccharides. Strongly stained M4B lumen with Periodic acid–Schiff (PAS) reagent in M4B paraffin sections confirmed HMW complexes with polysaccharide components. Furthermore, M4B smears stained with Periodic acid–Schiff revealed the presence of polysaccharide fibers. Therefore, we propose a key digestive mechanism of M4B: bacteriolytic fibers, polysaccharide fibers associated with digestive enzymes such as cathepsin L, specialized for Burkholderia symbionts in Riptortus gut symbiosis.
Genome reduction occurred in early Prochlorococcus with an unusually low effective population size
Authors: Zhang Hao , Hellweger Ferdi L , Luo Haiwei 
Published: January 2024

Abstract
In the oligotrophic sunlit ocean, the most abundant free-living planktonic bacterial lineages evolve convergently through genome reduction. The cyanobacterium Prochlorococcus responsible for 10% global oxygen production is a prominent example. The dominant theory known as “genome streamlining” posits that they have extremely large effective population sizes (Ne) such that selection for metabolic efficiency acts to drive genome reduction. Because genome reduction largely took place anciently, this theory builds on the assumption that their ancestors’ Ne was similarly large. Constraining Ne for ancient ancestors is challenging because experimental measurements of extinct organisms are impossible and alternatively reconstructing ancestral Ne with phylogenetic models gives large uncertainties. Here, we develop a new strategy that leverages agent-based modeling to simulate the changes in the genome-wide ratio of radical to conservative nonsynonymous nucleotide substitution rate (dR/dC) in a possible range of Ne in ancestral populations. This proxy shows expected increases with decreases of Ne only when Ne falls to about 10 k − 100 k or lower, magnitudes characteristic of Ne of obligate endosymbiont species where drift drives genome reduction. Our simulations therefore strongly support a scenario where the primary force of Prochlorococcus genome reduction is drift rather than selection.
Flexible genomic island conservation across freshwater and marine Methylophilaceae
Authors: Layoun Paul , López-Pérez Mario , Haro-Moreno Jose M , Haber Markus , Thrash J Cameron , Henson Michael W , Kavagutti Vinicius Silva , Ghai Rohit , Salcher Michaela M 
Published: January 2024

Abstract
The evolutionary trajectory of Methylophilaceae includes habitat transitions from freshwater sediments to freshwater and marine pelagial that resulted in genome reduction (genome-streamlining) of the pelagic taxa. However, the extent of genetic similarities in the genomic structure and microdiversity of the two genome-streamlined pelagic lineages (freshwater “Ca. Methylopumilus” and the marine OM43 lineage) has so far never been compared. Here, we analyzed complete genomes of 91 “Ca. Methylopumilus” strains isolated from 14 lakes in Central Europe and 12 coastal marine OM43 strains. The two lineages showed a remarkable niche differentiation with clear species-specific differences in habitat preference and seasonal distribution. On the other hand, we observed a synteny preservation in their genomes by having similar locations and types of flexible genomic islands (fGIs). Three main fGIs were identified: a replacement fGI acting as phage defense, an additive fGI harboring metabolic and resistance-related functions, and a tycheposon containing nitrogen-, thiamine-, and heme-related functions. The fGIs differed in relative abundances in metagenomic datasets suggesting different levels of variability ranging from strain-specific to population-level adaptations. Moreover, variations in one gene seemed to be responsible for different growth at low substrate concentrations and a potential biogeographic separation within one species. Our study provides a first insight into genomic microdiversity of closely related taxa within the family Methylophilaceae and revealed remarkably similar dynamics involving mobile genetic elements and recombination between freshwater and marine family members.
Strain-level diversity in sulfonamide biodegradation: adaptation of Paenarthrobacter to sulfonamides
Authors: Huang Yue , Pan Anxin , Song Ying , Deng Yu , Wu Alnwick Long-Hei , Lau Colin Shiu-Hay , Zhang Tong 
Published: January 2024

Abstract
The widespread occurrence of sulfonamides raises significant concerns about the evolution and spread of antibiotic resistance genes. Biodegradation represents not only a resistance mechanism but also a clean-up strategy. Meanwhile, dynamic and diverse environments could influence the cellular function of individual sulfonamide-degrading strains. Here, we present Paenarthrobacter from different origins that demonstrated diverse growth patterns and sulfonamide-degrading abilities. Generally, the degradation performance was largely associated with the number of sadA gene copies and also relied on its genotype. Based on the survey of sad genes in the public database, an independent mobilization of transposon-borne genes between chromosome and plasmid was observed. Insertions of multiple sadA genes could greatly enhance sulfonamide-degrading performance. Moreover, the sad gene cluster and sadA transposable element showed phylogenetic conservation currently, being identified only in two genera of Paenarthrobacter (Micrococcaceae) and Microbacterium (Microbacteriaceae). Meanwhile, Paenarthrobacter exhibited a high capacity for genome editing to adapt to the specific environmental niche, opening up new opportunities for bioremediation applications.
PSVII-30 Effect of mineral mixture containing HMTBa in hay-based substrate on methane production.
Authors: Figueiredo Cibelle Borges , Ferreira Reginaldo Nassar , Ubeda Emanuely Zequim , Nunes Gabriela Vitória Pinto , Miranda Matheus Alves , Filho Edson Martins Ribeiro 
Published: January 2024

Abstract
In grazing systems, cattle produce a high molar ratio of acetate, resulting in greater methane production and greater energy loss via gas production. Thus, the objective of this study was to evaluate in vitro gas production and methane concentration using a mineral mixture containing 0.4% HMTBa in a Tifton hay-based substrate. The experiment was carried out under anaerobic conditions using ANKOM Gas Pressure Monitor bottles kept at 39 ºC containing artificial saliva, ruminal fluid, and two grams of a substrate from each treatment: (FE) Control, 1.75 g of Tifton hay and 0.25 g of mineral mixture; and (FM) 1.75 g of Tifton hay and 0.25 g of Suplemax mineral mixture (Suplemento Nutrição Animal) containing 0.4% of HMTBa. Duplicate substrates and duplicate blanks were fermented for each treatment in two runs, with a total of four repetitions per treatment. The accumulated pressure resulting from the fermentation was measured automatically over 48 hours using the Gas Monitor software connected to the ANKOM modules of each bottle. The conversion of pressure to gas volume was calculated as follows: Volume (mL) = 2.3932 + 3.4053 × pressure (R2 = 0.9146; n = 1.151). At the end of each run, a gas sample was collected from each bottle to measure methane concentration using gas chromatography. To obtain the methane production for every 100 mL of gas produced, the total volume of methane produced was multiplied by 100 and divided by the total gas production. Data were analyzed in a completely randomized design using analysis of variance (ANOVA) at α = 0.05. Gas production was not affected by the inclusion of HMTBa (P = 0.3938), with an average of 54.14 mL (Table 1). There was a reduction in methane production from 25.41 to 20.05 mL with the addition of HMTBa (P = 0.0034), representing a decrease of 21.09%. There was a tendency to reduce methane production for each 100 mL of gas produced from 46.01 to 37.86 mL (P = 0.0889). Although HMTBa reduces the molar proportion of propionate, it is considered to be one of the main hydrogen users. In environments with a high concentration of H2 (above 1 mmol) and the presence of an anti-methanogen, the most favored path for using hydrogen is acetogenesis, which reduces methane production. Thus, the use of a mineral mixture containing 0.4% HMTBa in a Tifton-hay-based substrate reduced methane production in an in vitro assay.
Prevalence and Trend of Depression in Burn Survivors: A Single Center Cohort Study
Authors: Ranganath V S , Segu Smitha , Girish B S , Johns Joel M , Meghana C S 
Published: January 2024

Abstract
Burn injuries are frequently disastrous catastrophes that can cause significant skin damage and frequently result in hypertrophic scarring. Recovery from burn injury includes 3 domains—medical, functional, and psychiatric. Psychological concerns affect both the quality of life and the disease recovery. This study assesses the prevalence of major depression or clinically significant symptoms of depression among patients hospitalized for acute burn injury, as well as their persistence once diagnosed. It is a prospective cohort that was studied in 1%-40% of patients with body surface area burn, where the Beck Depression Inventory was used as a depressive symptom measure. A total of 108 patients were included in the study, out of which 42 (38.8%) exhibited depressive symptoms. Type of burn did not affect the prevalence of depression, whereas total body surface area had an impact on prevalence rate. The Beck Depression Inventory score assessment during the study revealed that 8.4% of participants exhibited worsening of scores during the study period and only 3.4% showed improvement throughout the study. Patients with mild depressive symptoms at admission were likely to recover, whereas those with severe depressive symptoms were less likely to recover from the psychological state. To conclude, depression was more prevalent among burn survivors from the Indian community.
Factors Predicting Burn Surgery Operative Time in a Middle-Income Country Regional Burn Service
Authors: Marais Marlé , Wall Shelley , Smith Michelle , Allorto Nikki 
Published: January 2024

Abstract
Access to theater is essential for the surgical management of deep burns. This is a scarce resource in low-middle-income countries. It underpins the importance of optimizing theater time. We sought to look at specific factors and their influence on operative time in minor to moderate burn surgery. This knowledge can assist teams where theater planning and optimization may be beneficial in resource-limited settings. Operative records between January and December 2018 at the Regional Hospital were analyzed. Data fields included age, gender, TBSA of burn, surface area burn operated on, intraoperative position change, seniority of surgeon, presence of an assistant, inclusion of special areas, predicted operative time, and actual operative time. Operative records for 265 patients were analyzed, with a median operative time of 40 minutes (interquartile range 25-64). Overall factors that predict longer operating time are larger total body surface area burn, larger surface area burn operated on, an operation involving at least one special area, number of special areas operated on, position change, presence of an assistant, and longer predicted operative time. Total percentage burn, operated percentage, special areas to be operated, and position change are overall factors to be considered when planning a burns list for the nonspecialist burn surgeon. This knowledge may be useful for an inexperienced surgeon to understand and aid in the effective utilization of limited operative time available for the surgical management of deep burns in resource-limited settings.
Morals in Multi-Unit Markets
Authors: Ziegler Andreas G B , Romagnoli Giorgia , Offerman Theo 
Published: January 2024

Abstract
We examine how the erosion of morals, norms, and norm compliance in markets depends on the market power of individual traders. Previously studied markets allow traders to exchange at most one unit and provide market power to individual traders by de-activating two forces: (i) the replacement logic, whereby immoral trading is justified by the belief that others would trade otherwise and (ii) market selection, by which the least moral trader determines aggregate quantities. In an experiment, we compare single-unit to (more common) multi-unit markets, which may activate these forces. Multi-unit markets, in contrast to single-unit markets, lead to a complete erosion of morals. This is associated primarily with a deterioration in norm compliance: the observed level of immoral trade is in contrast with the prevailing social norm. The replacement logic is the main mechanism driving this finding.
Human Rights-based Approaches and the Right to Health: A Systematic Literature Review
Authors: Patterson David 
Published: January 2024

Abstract
The term ‘human rights-based approach’ is common in rights and international development literature. Yet there is no single, universally agreed definition of a human rights-based approach, let alone its application to the right to health. This article uses a PRISMA-informed systematic literature review to address the question, ‘What is the current status of the human rights-based approach to health in international law?’ Previous reviews have described how international organizations and development donors have tackled human rights-based approaches to development generally and discussed prominent works on human rights-based approaches to health. However, this is the first review to sample the peer-reviewed literature systematically. The study revealed that authors use the terms ‘human rights’, ‘human rights-based approach’ and ‘right to health’ to import a raft of legal implications, or none at all. Similarly, readers may assign legal meanings to these terms, or none at all. Confusion arises because although these terms often have different meanings for authors and readers from different disciplines, this is not commonly acknowledged, and authors rarely clarify their perspectives. The author concludes that scholars should seek co-authors with human rights law or public health qualifications, as relevant. Most academic institutions research and teach health and law separately; interdisciplinary centres of excellence in health, law and human rights offer an opportunity to overcome these historical obstacles to interdisciplinary dialogue and understanding. The study and its conclusions will be of interest to legal researchers, human rights advocates, public health scholars, and advocates from other disciplines.
When the Dragon comes Home to Roost: Chinese Investments in the EU, National Security, and Investor–State Arbitration
Authors: Gáspár-Szilágyi Szilárd 
Published: January 2024

Abstract
The gradual rise of China as an economic, normative, and lending power has resulted in more protectionist measures in areas of the world that traditionally championed economic liberalization. Currently, 21 out of 27 European Union (EU) Member States have national laws or measures in place for the screening or review of foreign investments. However, such restrictive national measures can result in investment treaty-based arbitration under the existing bilateral investment treaties concluded by 26 EU Member States with China, as evidenced by the recent arbitration initiated by Huawei against Sweden. Therefore, this article assesses whether EU Member States are likely to see a spike in investor–State arbitral claims initiated by Chinese investors as a result of the former’s investment screening measures. To achieve this aim, the article first looks at the bilateral investment treaties (BIT)-level variables that can influence the initiation of arbitration against EU Member States, such as the presence and type of investor–State arbitration (ISA) clauses, the types of investments being made, the coverage of the pre- and/or post-establishment phases, or the inclusion of ‘non-precluded measures’ clauses. This is then followed by a look at other variables, such as the decreasing number of Chinese foreign direct investment into EU countries, the treatment of Chinese investors in recent high-profile cases, and the importance of security alliances. The article concludes that those EU States are at a higher risk of being respondents in arbitrations initiated by Chinese investors whose BITs with China include modern ISA clauses, cover the pre-establishment phase, and lack non-precluded measures clauses. However, EU States should wait for the outcome of the Huawei v Sweden arbitration before deciding whether the amendment or termination of the existing BITs with China is needed.
Perceptions of Tree Diseases in Indigenous Communities: Native Alaskan and Hawaiian Insights
Authors: Roy Kylle , Frankel Susan J , Oakes Lauren E , Francisco Kainana S , Kealiʻikanakaʻoleohaililani Kekuhi , Sitz Rachael A , Huff Emily S , Schelhas John 
Published: January 2024

Abstract
Concern for tree mortality and damage, and preferences for management interventions, reflect people’s values and cultures. We examine perceptions of tree diseases in two Indigenous communities to provide examples of the types of distress and attitudes that arise when tree diseases significantly damage cultural values. Summaries of yellow-cedar decline as perceived by Tlingit and Haida Alaska Natives and rapid ʻōhiʻa death (ROD) and Native Hawaiian culture underscore the interconnections between local people and local forests and the ways forest diseases can impart a loss of culture and connections to ancestors. The complex relationships between Indigenous people, ecosystems, and tree diseases highlight the importance of the inclusion of diverse viewpoints to inform natural resource planning and management so that it may be conducted equitably and ethically.
Study Implications: Consideration of cultural and social impacts as criteria for prioritization of forest disease management requires knowledge of the types and intensity of impacts that forest diseases have on Indigenous and Western culture. A greater understanding of the diversity of perspectives toward forest diseases is needed to inform natural resource planning and management so that it may be conducted in an ecologically sound and inclusive manner.
Digital Rights and the State of Exception. Internet Shutdowns from the Perspective of Just Securitization Theory
Authors: Thumfart Johannes 
Published: January 2024

Abstract
Addressing cases from Cameroon, Egypt, Ethiopia, India, Indonesia, Iran, Nigeria, Pakistan, Spain, Togo, the United Kingdom, the United States, and Zimbabwe, this article discusses the global phenomenon of internet shutdowns (ISs) from the normative perspective of Just Securitization Theory (JST). It focuses on the conflict between arguments used to justify ISs and the negative impact of ISs regarding fundamental and human rights. This article develops strict criteria for when ISs might be legitimate as extraordinary security measures in emergency situations. Following JST, these criteria are based on citizens’ right to physical integrity, the expectation of reasonable success, proportionality, harm minimization, and specificity. I argue that it is not legitimate to use ISs to enact collective punishment, preemptive censorship, or hamper legitimate political protests. While denying the legitimacy of the vast majority of ISs on these grounds, I sketch four exceptional scenarios (“WhatsApp lynchings,” “US Capitol 2021,” “Computer virus,” and “False alarm”) in which ISs can be legitimate. JST also includes states’ duty to desecuritize once a threat has been neutralized. In this way, a balanced discussion of ISs as an exceptional measure from the perspective of JST contributes to the establishment of a customary positive human right to digital connectivity in the normal situation.