bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract A006: Using ChatGPT to solve clinical radiobiology problems
Authors: Kao Yung-Shuo , Su Che-Wei , Dai Kun-Yao 
Published: January 2025

Abstract
ChatGPT is a powerful large language model and has been applied to many fields in medicine. Radiobiology is the key in the determining of radiation dose in radiation oncology. We would like to test the efficacy of using ChatGPT in clinical radiobiology. A set of 30 clinical radiobiology questions were used in this study. OpenAI API was used to query GPT-4O and generate corresponding answers (Access Date: 2024/6/26). Five different prompts (involving role-playing, role-playing with in-context learning, chain of thought, chain of thought with in-context training, and in-context training) and no prompt were used to test the ability of the model. Questions were further divided into two subgroups, 15 questions without alpha-beta ratio and 15 questions with alpha-beta ratio(s). Answers were graded using the below grading system: Grade 1: totally incorrect; Grade 2: partially correct (either answer or calculation process is correct); Grade 3: totally correct (both answer and calculation process are correct). The chi-square test was used to compare results between different situations. R studio and R language were used in the statistical analysis; p &lt;0.05 is set as statistically significant. The detailed results for each prompt are shown below: No prompt(grade 1: 9, grade 2: 3, grade 3: 18), role-playing (grade 1: 7, grade 2: 6, grade 3: 17), role-playing with in-context learning(grade 1: 10, grade 2: 7, grade 3: 13), chain of thought(grade 1: 10, grade 2: 5, grade 3: 15), chain of thought with in-context learning(grade 1: 11, grade 2: 2, grade 3: 17), and in-context learning(grade 1: 10, grade 2: 4, grade 3: 16). For grade 1 versus grade 2-3, Chi-square test showed p-value = 0.6708, and for grade 1-2 versus grade 3, chi-square test showed p-value = 0.829. That meant that the performances were similar regardless of the prompt. The detailed results for two question types are shown below: questions without alpha-beta ratio (grade 1: 53, grade 2: 10, grade 3: 27), questions with alpha-beta ratio(s) (grade 1: 4, grade 2: 17, grade 3: 69). For grade 1 versus grade 2-3, Chi-square test showed p-value &lt;0.01, and for grade 1-2 versus grade 3, chi-square test showed p-value &lt;0.01. That meant that the performances were different between question with and without alpha-beta ratio(s). In conclusion, without prompting, ChatGPT can correct about 60% of questions. Using different prompts cannot enhance performance. ChatGPT can perform well in questions with alpha-beta ratio(s).
Citation Format: Yung-Shuo Kao, Che-Wei Su, Kun-Yao Dai. Using ChatGPT to solve clinical radiobiology problems. [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Targeted Therapies in Combination with Radiotherapy; 2025 Jan 26-29; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2025;31(2_Suppl):Abstract nr A006.
Abstract A011: Activity of antibody-drug conjugates with radiation in preclinical bladder cancer models
Authors: Zhou Yuzhen , Shanmugam Surish , D'Andrea Vincent , Stelter Isabella , Stormoen Dag , Chroneos Rea , Hanlon Timothy , Epstein Ilana , Bekele Raie , Anderson William , Szallasi Zoltan , Bellmunt Joaquim , Mouw Kent 
Published: January 2025

Abstract
Antibody-drug conjugates (ADCs) are transforming the treatment landscape in metastatic bladder cancer, but the role of ADCs in localized disease is uncertain. Radiation is a central component of trimodality therapy (TMT), a curative-intent treatment approach for muscle-invasive bladder cancer (MIBC) that includes concurrent radiation and cytotoxic chemotherapy. We investigated the safety and efficacy of combining novel ADCs with radiation in preclinical bladder cancer models. We observed additive cytotoxicity in vitro, and the combination of ADCs with conformal fractionated radiation was well-tolerated and resulted in improved tumor control compared to either agent alone in vivo. We also assessed the impact of DNA repair pathway alterations commonly observed in bladder tumors on sensitivity to ADCs alone and in combination with radiation and DNA repair targeted agents. Homologous recombination (HR) deficiency modestly sensitized bladder cancer cells to MMAE, the payload of the ADC enfortumab vedotin (EV), and potently sensitized cells to govitecan, the payload of the ADC sacitumuzab govitecan (SG). Conversely, nucleotide excision repair (NER) deficiency did not sensitize cells to either MMAE or govitecan. The combination of govitecan and PARP inhibition resulted in synergistic cell killing independent of HR or NER status. Together these data provide preclincal support for clinical efforts to combine ADCs with radiation in MIBC and identify rationale combination therapy approaches.
Citation Format: Yuzhen Zhou, Surish Shanmugam, Vincent D'Andrea, Isabella Stelter, Dag Stormoen, Rea Chroneos, Timothy Hanlon, Ilana Epstein, Raie Bekele, William Anderson, Zoltan Szallasi, Joaquim Bellmunt, Kent Mouw. Activity of antibody-drug conjugates with radiation in preclinical bladder cancer models. [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Targeted Therapies in Combination with Radiotherapy; 2025 Jan 26-29; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2025;31(2_Suppl):Abstract nr A011.
Abstract B012: Interferon- γ is required for immunotherapy induced radiosensitivity in solid tumors
Authors: Wu Annette , Kang Minjeong , Lee Daeyong , Wang Yifan , Huntoon Kristin M. , Kim Betty Y.S. , Jiang Wen 
Published: January 2025

Abstract
Radiation therapy (RT) is the standard of care for many cancer types, however, some solid tumors fail to respond due to factors such as a hypoxic tumor microenvironment (TME). To overcome radioresistance in these solid tumors, clinical trials combining RT with immune checkpoint blockade (ICB) are ongoing, but hindered by a limited understanding of the mechanisms by which immunotherapies enhance RT response. In this study, we use EO771 breast and CT26 colorectal tumor models to investigate how anti PD-1 therapy improves radiosensitivity to varying RT regimens including single or multiple RT doses administered concurrently or sequentially. We show that concurrent administration of ICB and RT normalizes tumor vasculature, alleviates hypoxia, and increases vessel perfusion to improve immune cell infiltration in solid tumors. Furthermore, we reveal that this TME modulation was linked to stimulation of interferon-γ (IFN-γ) signaling in endothelial cells by activated CD8+ T cell responses, which results in systemic tumor control and establishes long term immunological memory in the neoadjuvant setting. Collectively, these results indicate that immunotherapy sensitizes solid tumors to RT by modulating the tumor microenvironment in an IFN-γ dependent manner, resulting in successful anti-tumor immunity. This research provides a rationale for the concurrent administration of anti PD-1 therapy and radiation therapy to treat radioresistant solid tumors.
Citation Format: Annette Wu, Minjeong Kang, Daeyong Lee, Yifan Wang, Kristin M. Huntoon, Betty Y.S. Kim, Wen Jiang. Interferon- γ is required for immunotherapy induced radiosensitivity in solid tumors. [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Targeted Therapies in Combination with Radiotherapy; 2025 Jan 26-29; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2025;31(2_Suppl):Abstract nr B012.
Abstract IA01: Moving forward translation of targeted therapy with external beam radiation
Authors: Baumann Michael 
Published: January 2025

Radiotherapy is an anatomically highly targeted treatment modality capable of eradicating cancer cells, including cancer stem cells (CSC). This precision makes radiotherapy an ideal partner for synergistic treatment with targeted drugs, combining local effects on the tumor with systemic molecular interventions. To evaluate the efficacy of such combined treatments on inactivation of CSC in translational research, it is essential to select the appropriate clinical endpoints for preclinical experiments, most importantly inactivation of CSC in vitro or local tumor control in vivo. This ensures that preclinical findings are better translatable to the clinical setting, bridging the gap between experimental models and patient outcomes. A critical factor in this translational pipeline is the use of appropriate in vitro and animal or explant models that as accurate as possible mimic the clinical tumor microenvironment and response dynamics. To account for inter-tumor heterogeneity, it is necessary to apply a panel of models that are well characterized on a molecular biological level. With the right preclinical systems in place, combination strategies can be refined to optimize timing, dosing and sequencing of therapies to maximize tumor control while minimizing damage to normal tissues. Biomarker-driven approaches and molecular pathway analysis are increasingly guiding patient selection and therapeutic personalization, thereby improving the likelihood of clinical success. Examples include biomarker-based studies such as the DELPHI trial for dose de-escalation in post-op HPV-positive head and neck cancer (HNSCC) and the INDIRA Miso study on escalation of PET hypoxic HNSCC. Promising examples of novel combinations with radiotherapy include not only small molecule or Immunotherapeutics, but also the use of radiotherapy integration with novel theranostic radiopharmaceuticals, genomic and radiomic data for adaptive treatment planning. By aligning preclinical and clinical strategies, these efforts are advancing the integration of targeted therapies with radiotherapy and improve loco-regional control, overall survival and quality of life for patients.
Citation Format: Michael Baumann. Moving forward translation of targeted therapy with external beam radiation. [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Targeted Therapies in Combination with Radiotherapy; 2025 Jan 26-29; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2025;31(2_Suppl):Abstract nr IA01
Abstract IA06: Targeting ferroptosis in radioresistance
Authors: Gan Boyi 
Published: January 2025

Abstract
Ferroptosis is a distinct form of regulated cell death driven by iron-dependent lipid peroxidation, which disrupts cellular homeostasis and viability. Recent studies, including our own, have demonstrated that radiotherapy (RT) is a potent inducer of ferroptosis. These findings have uncovered a critical link between ferroptosis and RT-mediated tumor suppression. Moreover, we and others have identified ferroptosis inducers (FINs) as promising radiosensitizers that enhance the efficacy of RT, particularly in tumors exhibiting intrinsic or acquired radioresistance. In this presentation, I will provide an overview of the fundamental biology of ferroptosis, emphasizing its unique characteristics and mechanisms. I will highlight our discoveries on the role of ferroptosis in RT-mediated cell death and tumor control. Additionally, I will delve into the mechanisms underlying ferroptosis-driven radiosensitization and present evidence supporting the use of FINs as a novel strategy to overcome radioresistance. Finally, I will discuss the potential clinical implications of combining FINs with RT, offering insights into how these advancements might improve outcomes for patients with challenging radioresistant tumors.
Citation Format: Boyi Gan. Targeting ferroptosis in radioresistance. [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Targeted Therapies in Combination with Radiotherapy; 2025 Jan 26-29; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2025;31(2_Suppl):Abstract nr IA06
Bacurau: A arte de entrelaçar imagens, sons e memórias no processo de criação cinematográfica
Authors: Ferraraz Rogério , Magno Maria Ignes Carlos 
Published: January 2025

Bacurau – pássaro comum no Brasil, de plumagem muito macia, voo silencioso, olhos muito brilhantes e só encontrado à noite – deu nome ao filme de Kleber Mendonça Filho e Juliano Dornelles. O filme conta a história de um povoado do sertão brasileiro que misteriosamente desaparece do mapa e é invadido por drones e estranhos turistas que começam a atacar as pessoas do lugar. A comunidade precisa identificar o inimigo e criar coletivamente um meio para se defender. Na ficção, Bacurau sumiu do mapa e se tornou apenas uma lembrança sem referência geográfica, mas na história contada Bacurau está repleto de referências culturais, alusões cinematográficas e lembranças fílmicas, nacionais e internacionais, e pede que comecemos a querer ligar “os fios da meada no rio” criativo do filme. Essa é a nossa proposta de resenha: refletir sobre esse processo de criação do fazer cinema.
Accumulation of advanced oxidative protein products exacerbate satellite glial cells activation and neuropathic pain
Authors: Tu Chen , Wang Shi-Cheng , Dai Meng-Xuan , Lai Si-Qi , Huang Zhi-Wei , Yu Yong-Peng , Chen Yun-Biao , Zeng Ji-Huan , Wang Liang , Zhong Zhao-Ming 
Published: January 2025

Abstract

Background
Neuropathic pain (NP) is a debilitating condition caused by lesion or dysfunction in the somatosensory nervous system. Accumulation of advanced oxidation protein products (AOPPs) is implicated in mechanical hyperalgesia. However, the effects of AOPPs on NP remain unclear.

Methods
A rat model of NP was established by chronic constriction injury (CCI) and employed to evaluate the changes of mechanical withdrawal threshold, thermal and cold withdrawal latency, as well as AOPPs levels. The effects of AOPPs on the activation of satellite glial cells (SGCs) in the dorsal root ganglion (DRG), receptor for advanced glycation end-products (RAGE) expression, and NF-κB signaling pathway activation were also investigated using western blotting, immunofluorescence, and the Fluo4-AM fluorescence probe for calcium signaling. Additionally, oxidative stress levels and inflammatory cytokine production in SGCs, triggered by AOPPs exposure, were measured through the DCFH-DA probe for ROS detection and ELISA kits for cytokine quantification.

Results
CCI significantly elevated the AOPPs levels in the plasma and sciatic nerve and caused AOPPs accumulation in the DRG. Exogenous AOPPs activated SGCs, increased reactive oxygen species and inflammatory response, upregulated the RAGE, and activated NF-κB signaling. The RAGE inhibitor FPS-ZM1 effectively inhibited AOPPs-induced SGC activation. Additionally, AOPPs intervention worsened CCI-induced hyperalgesia and neuroinflammation in vivo.

Conclusion
These results indicate that AOPPs exacerbate the SGC activation and NP following nerve injury, and AOPPs accumulation might play an important role in the pathogenesis of NP.
Analysis of logistics measures of CEP service providers for the last-mile delivery in small- and medium-sized cities: A case study for the Aachen city region
Authors: Ghazal Abdulrahmon , Narayanan Santhanakrishnan , Adeniran Ibraheem Oluwatosin , Kehrt Carina , Antoniou Constantinos 
Published: January 2025

Abstract
The e-commerce sector’s rapid expansion has led to an increase in delivery activities both within and across cities, fuelling the growth of the courier, express, and parcel (CEP) services. CEP service providers are crucial for the distribution of goods across all types of cities, especially for last-mile delivery. However, CEP service providers need innovative approaches for their last-mile distribution in small- and medium-sized cities to reduce transport costs and negative environmental impacts. For this reason, this paper analyses the quantitative impacts of logistics measures of CEP service providers for last-mile delivery in small- and medium-sized cities, especially the resulting transport costs and environmental impacts, in the framework of a case study for the investigation area of the Aachen city region. A simulation-based analysis was conducted using the agent-based transport simulation MATSim and the linked route optimisation Jsprit. The results revealed that electric trucks are not cost-effective as a stand-alone logistics measure for last-mile delivery in small- and medium-sized cities. However, combining electric trucks with other sustainable logistics measures, such as parcel shops and parcel lockers, results in a viable logistics measure for last-mile delivery. It is possible to reduce total transport costs by at least 5.4% and CO2 emissions by at least 61.1%. Hence, CEP service providers should replace diesel trucks with a mix of sustainable logistics measures for last-mile delivery in small- and medium-sized cities to achieve better operational efficiency and lesser environmental impacts.
Future perspectives for PDE5 inhibitors bridging the gap between cardiovascular health and psychological status
Authors: Sansone Andrea , Guida Eugenia , Dolci Susanna , Frangione Valeria , Asso Amanda , Bellia Gilberto , Jannini Emmanuele A. 
Published: January 2025

Abstract
The serendipitous discovery that inhibiting type 5 phosphodiesterase (PDE5) using sildenafil, a potent PDE5 inhibitor (PDE5i) initially developed for cardioprotection, introduced the possibility of orally managing erectile dysfunction (ED) led to an increase in research data, which are currently considered groundbreaking for the new discipline of sexual medicine. Findings from a number of laboratories and clinics around the world unanimously demonstrated the following: (i) the major cause of ED is directly or indirectly related to cardiovascular disease (CVD); (ii) ED and CVDs share the same risk factors, which are related mainly to lifestyle choices; (iii) the first therapeutic approach to both ED and CVDs is to transform harmful lifestyles into virtuous lifestyles; and (iv) PDE5is in general, particularly sildenafil, are very safe, if not protective, for use in CVD patients. However, the use of PDE5is has faced several challenges. Many patients and some healthcare providers (HCPs) often share the misconception that using these drugs can increase the risk of CVD. Some patients might desire to fulfill the unmet need for privacy linked to the stigma of being treated for ED or might be enticed by the idea of buying drugs online, either because of shame or cheaper prices, without knowing the risks associated with counterfeit drugs. The aim of this narrative revision of the current literature is to demonstrate that (i) the orodispersible film of sildenafil is safe from a CV perspective; (ii) it is a discreet formulation that respects the need for privacy; and (iii) it is virtually the unique PDE5i formulation too expensive to produce outside the correct channels, making it impossible to be counterfeit.
Factors influencing role preferences in decision-making of healthy women with BRCA1/2 pathogenic variants: subanalysis from a randomised controlled decision coaching trial
Authors: Kautz-Freimuth Sibylle , Shukri Arim , Stracke Claudia , Isselhard Anna , Berger-Höger Birte , Steckelberg Anke , Vitinius Frank , Dikow Nicola , Kiechle Marion , Meisel Cornelia , Wöckel Achim , von Mackelenbergh Marion Tina , Schmutzler Rita , Rhiem Kerstin , Stock Stephanie 
Published: January 2025

Abstract

Background
Patients who actively engage in their medical decision-making processes can experience better health outcomes. This exploratory study aimed to identify predictors of preferred and actual roles in decision-making in healthy women with BRCA1/2 pathogenic variants (PVs).

Methods
Women with BRCA1/2 PVs without a history of breast and/or ovarian cancer were recruited in six centres across Germany. Those returning the baseline questionnaires (T1) were randomly assigned to the intervention or control group (IG, CG). The IG completed a decision-coaching (DC) programme, the CG received standard care. A second survey (T2) followed after 12 weeks. Ordinal regression analyses were performed. Sociodemographic and outcome-related baseline variables were used to identify predictors of (i) desired role at T1 in the total group and (ii) actual role at T2 in the CG and the IG. Role preferences were measured with the Control Preferences Scale.

Results
389 women completed the baseline questionnaires, 191 were randomised to the CG and 198 to the IG. At T1, high decisional conflict (OR 1.016, 95% CI 1.001–1.023, p = 0.038) and a negative self-concept (OR 1.030, 95% CI 1.008–1.054, p = 0.009) were significant predictors for preferring a more passive role. At T2, high baseline decisional conflict significantly predicted taking a more passive role in the CG, whereas in the IG, baseline decisional conflict showed no influence. Furthermore, in the IG, younger age (OR 1.049, 95% CI 1.001–1.098, p = 0.044) and a non-academic education (OR 0.46, 95% CI 0.213–0.775, p = 0.006) were identified as significant predictors for taking a more active role.

Conclusions
High initial decisional conflict was identified as an important predictor for preferring and taking a passive role in decision-making among women with BRCA1/2 PVs. Participating in the DC programme can counteract passivating effects of an initially high decisional conflict and particularly support younger PV carriers and those with lower educational status to take an active role. With this profile, the DC programme expands the existing counselling and care concept to include a measure that can also specifically cover the support needs of younger women and those with a lower education level.

Trial registration
DRKS-ID: DRKS00015527. Registered 30/10/2019.