bluuebunny/crossref_metadata_2025_split huggingface dataset:
The science of rural human settlements: a comprehensive overview
Authors: Liu Junyou , Zheng Bohong , Tang Haifang 
Published: December 2023

In the process of rapid urbanization in the world, many villages are facing a series of problems such as depopulation, population ageing, insufficient infrastructure, and public service facilities. In order for better rural development and full implementation of the 2030 sustainable development goals, we should hammer at improving the rural human settlements. Based on the science of human settlements proposed by Constantinos Doxiadis, bibliometric analysis was made on studies related to rural human settlements in the last 25 years. Through analysis, we believe that the number of related studies will gradually increase. Currently, the improvement of the rural ecological environment and the creation of a healthy rural human settlement environment are two hot topics. It is very likely that these topics will also be highly focused in the following decades. With the gradual development of rural areas and the improvement of the material lives of people, more and more scholars will pay attention to higher-level demands such as rural cultural environment and self-realization of villagers. By reviewing literature related to rural human settlements, we can comprehensively consider different aspects in rural human settlements. This is quite beneficial for the comprehensive development of rural human settlements. Scholars in different countries have similar focuses related to rural human settlements. However, because of different country conditions, the focuses of researchers have some obvious differences. Relevant literature contributes to the development of the science of human settlements.
Age-related changes in human bone marrow mesenchymal stromal cells: morphology, gene expression profile, immunomodulatory activity and miRNA expression
Authors: Massaro Fulvio , Corrillon Florent , Stamatopoulos Basile , Dubois Nathan , Ruer Achille , Meuleman Nathalie , Bron Dominique , Lagneaux Laurence 
Published: December 2023

IntroductionMesenchymal stromal cells (MSC) are one of the main cellular components of bone marrow (BM) microenvironment. MSC play a key role in tissue regeneration, but they are also capable of immunomodulating activity. With host aging, MSC undergo age-related changes, which alter these functions, contributing to the set-up of “inflammaging”, which is known to be the basis for the development of several diseases of the elderly, including cancer. However, there’s few data investigating this facet of MSC, mainly obtained using murine models or replicative senescence. The aim of this research was to identify morphological, molecular and functional alterations of human bone marrow-derived MSC from young (yBM-MSC) and old (oBM-MSC) healthy donors.MethodsMSC were identified by analysis of cell-surface markers according to the ISCT criteria. To evaluate response to inflammatory status, MSC were incubated for 24h in the presence of IL-1β, IFN-α, IFN-ɣ and TNF-α. Macrophages were obtained by differentiation of THP-1 cells through PMA exposure. For M1 polarization experiments, a 24h incubation with LPS and IFN-ɣ was performed. MSC were plated at the bottom of the co-culture transwell system for all the time of cytokine exposure. Gene expression was evaluated by real-time PCR after RNA extraction from BM-MSC or THP-1 culture. Secreted cytokines levels were quantitated through ELISA assays.ResultsAging MSC display changes in size, morphology and granularity. Higher levels of β-Gal, reactive oxygen species (ROS), IL-6 and IL-8 and impaired colony-forming and cell cycle progression abilities were found in oBM-MSC. Gene expression profile seems to vary according to subjects’ age and particularly in oBM-MSC seem to be characterized by an impaired immunomodulating activity, with a reduced inhibition of macrophage M1 status. The comparative analysis of microRNA (miRNA) expression in yBM-MSC and oBM-MSC revealed a significant difference for miRNA known to be involved in macrophage polarization and particularly miR-193b-3p expression is strongly increased after co-culture of macrophages with yBM-MSC.ConclusionThere are profound differences in terms of morphology, gene and miRNA expression and immunomodulating properties among yBM-MSC and oBM-MSC, supporting the critical role of aging BM microenvironment on senescence, immune-mediated disorders and cancer pathogenesis.
Comparative analysis of contemporary anti-double stranded DNA antibody assays for systemic lupus erythematosus
Authors: Bauer Claus-Juergen , Karakostas Pantelis , Weber Nadine , Behning Charlotte , Stoffel-Wagner Birgit , Brossart Peter , Dolscheid-Pommerich Ramona , Schäfer Valentin Sebastian 
Published: December 2023

ObjectiveElevated double-stranded DNA (dsDNA) antibody levels in blood serum are considered a disease-specific marker in systemic lupus erythematosus (SLE), correlate with disease activity and the incidence of lupus nephritis, and can be detected in up to 86% of all SLE cases. Despite the high clinical relevance, the variety of dsDNA antibody testing methods with heterogenous performance in clinical use remains challenging. This study is the first to prospectively investigate the performance of two of today’s most commonly applied anti-dsDNA testing methods head-to-head under real-world conditions, as well as their correlation with other clinical and serological disease parameters in SLE patients.MethodsIn this prospective study, all SLE patients undergoing treatment at the Department of Rheumatology at the University Hospital Bonn within a 13-months period (n=41) and control patients without connective-tissue disease (n=51) were consecutively enrolled and examined. For all study participants’ serum samples both anti-dsDNA-NcX enzyme-linked immunoassay testing EUROIMMUN, Luebeck, Germany) and the fluorescence immunoassay ELiA dsDNA (Thermo Fisher Scientific, Waltham, USA) were performed. In addition, demographic data, further laboratory values and disease activity parameters were recorded. Clinical disease activity was assessed by SLEDAI-2K.ResultsBoth assays showed high specificity (anti-dsDNA-NcX ELISA: 0.9, ELiA dsDNA: 0.959), but there were notable differences in sensitivity (anti-dsDNA-NcX ELISA: 0.51, ELiA dsDNA: 0.38). Pearsons’s correlation yielded a positive correlation between anti-dsDNA concentrations and CRP concentrations for the anti-dsDNA-NcX ELISA (R=0.22; p=0.038) and a mild-to-moderate inverse correlation between concentrations of anti-dsDNA and complement C4 for the ELiA dsDNA test (R=-0.22; p=0.045) when SLE and control patients were considered together. Other than, no significant correlation between anti-dsDNA concentrations and clinical or laboratory findings was found for either test procedure.ConclusionBoth anti-dsDNA antibody assays represent reliable examination methods with high specificity for the diagnosis of SLE that fulfill EULAR/ACR requirements. However, the anti-dsDNA-NcX ELISA showed superior sensitivity and significant correlation with disease activity (as measured by CRP concentrations).
mtDNA data reveal disparate population structures and High Arctic colonization patterns in three intertidal invertebrates with contrasting life history traits
Authors: Csapó Hedvig , Jabłońska Aleksandra , Węsławski Jan Marcin , Mieszkowska Nova , Gantsevich Michael , Dahl-Hansen Ida , Renaud Paul , Grabowski Michał 
Published: December 2023

IntroductionPost-glacial climate variation is known to have influenced the distribution of marine species in the North Atlantic. In particular, the Atlantic side of the Arctic has experienced strong fluctuations in both atmospheric and sea surface temperature, as well as seasonal ice coverage since the last glacial maximum (LGM). Here, we aim to unveil the phylogeography and historical demography of three rocky intertidal marine invertebrates showing a trans-Atlantic distribution and presently inhabiting the Arctic: Gammarus oceanicus, Littorina saxatilis and Semibalanus balanoides.MethodsWe used a large amount of mitochondrial DNA barcode data, both newly-obtained and stored in public databases. We performed phylogeographic and demographic analyses on 1119 G. oceanicus, 205 L. saxatilis, and 884 S. balanoides sequences.ResultsOur results show that all three of these boreal species have expanded their effective population sizes in the high Arctic Svalbard Archipelago since the LGM. Analyses investigating the origin of all these populations point to the eastern Atlantic.DiscussionBased on our results we conclude that the expansion of these boreal species to the Arctic possibly happened during an earlier warm cycle of the Holocene era, and is probably not the result of the recent ‘Atlantification’ of the Arctic. We also discuss the effects of dispersal potential on population structure as an important aspect of comparative biogeographical studies.
A real-world study on the safety profile of extended-interval dosing of immune checkpoint inhibitors for melanoma: a single-center analysis in Japan
Authors: Ito Takamichi , Kaku-Ito Yumiko , Ohno Fumitaka , Nakahara Takeshi 
Published: December 2023

BackgroundAnti-programmed death-1 (PD-1) antibodies are the mainstay for the treatment of unresectable or high-risk melanoma. However, real-world data on the safety profile of their extended-interval doses (EDs) are limited, particularly in Asian patients with melanoma.Materials and methodsIn this single-center retrospective study, we analyzed the risks of immune-related adverse events (irAEs) among 71 Japanese patients (36 males; mean age, 65.0 years) who received anti-PD-1 monotherapy for melanoma at our institute. Patients who were administered ipilimumab prior to anti-PD-1 monotherapy were excluded. Patients were divided into three groups: canonical-interval dose (CD) group (n = 50, body weight-based dosing or 240 mg Q2W for nivolumab and body weight-based dosing or 200 mg Q3W for pembrolizumab), ED group (n = 14, 480 mg Q4W for nivolumab and 400 mg Q6W for pembrolizumab), and dose-switch (DS) group (n = 7, upfront CD followed by ED).ResultsThe CD group received nivolumab more frequently in the metastatic setting. There were no significant differences in baseline characteristics among the three groups, including in sex, age, primary tumor site, tumor subtype, and follow-up period. irAEs occurred in 36.6% (26 patients) of all patients (32.0% of the CD group, 35.7% of the ED group, and 71.4% of the DS group), while severe (grade ≥ 3) irAEs occurred in only two patients, both of whom were in the CD group. Most of the irAEs occurred during the first 6 months of anti-PD-1 therapy and, interestingly, all of the irAEs in the DS group occurred before the switch (during the CD). There was no significant difference among the three groups in the probability of irAE estimated by the Kaplan–Meier method.ConclusionThese findings may highlight the safety of ED of anti-PD-1 monotherapy in the treatment of Asian patients with melanoma.
Adolescent social housing protects against adult emotional and cognitive deficits and alters the PFC and NAc transcriptome in male and female C57BL/6J mice
Authors: Lodha Jyoti , Brocato Emily R. , Nash McKenzie , Marcus Madison M. , Pais A. Chris , Pais Alex B. , Miles Michael F. , Wolstenholme Jennifer Theresa 
Published: December 2023

IntroductionAdolescence is a critical period in cognitive and emotional development, characterized by high levels of social interaction and increases in risk-taking behavior including binge drinking. Adolescent exposure to social stress and binge ethanol have individually been associated with the development of social, emotional, and cognitive deficits, as well as increased risk for alcohol use disorder. Disruption of cortical development by early life social stress and/or binge drinking may partly underlie these enduring emotional, cognitive, and behavioral effects. The study goal is to implement a novel neighbor housing environment to identify the effects of adolescent neighbor housing and/or binge ethanol drinking on (1) a battery of emotional and cognitive tasks (2) adult ethanol drinking behavior, and (3) the nucleus accumbens and prefrontal cortex transcriptome.MethodsAdolescent male and female C57BL/6J mice were single or neighbor housed with or without access to intermittent ethanol. One cohort underwent behavioral testing during adulthood to determine social preference, expression of anxiety-like behavior, cognitive performance, and patterns of ethanol intake. The second cohort was sacrificed in late adolescence and brain tissue was used for transcriptomics analysis.ResultsAs adults, single housed mice displayed decreased social interaction, deficits in the novel object recognition task, and increased anxiety-like behavior, relative to neighbor-housed mice. There was no effect of housing condition on adolescent or adult ethanol consumption. Adolescent ethanol exposure did not alter adult ethanol intake. Transcriptomics analysis revealed that adolescent housing condition and ethanol exposure resulted in differential expression of genes related to synaptic plasticity in the nucleus accumbens and genes related to methylation, the extracellular matrix and inflammation in the prefrontal cortex.DiscussionThe behavioral results indicate that social interaction during adolescence via the neighbor housing model may protect against emotional, social, and cognitive deficits. In addition, the transcriptomics results suggest that these behavioral alterations may be mediated in part by dysregulation of transcription in the frontal cortex or the nucleus accumbens.
Optimized complete cytoreduction in ovarian cancer through intraoperative real-time tumor visualization by 5-ALA – a case report
Authors: Tascón Padrón Laura , Egger Eva K. , Ralser Damian Johannes , Otten Lucia , Toksöz Özer-Altan , Kristiansen Glen , Stummer Walter , Mustea Alexander 
Published: December 2023

IntroductionComplete macroscopic cytoreduction represents the most important prognostic parameter for overall survival in ovarian cancer. This dogma remains tenacious despite significant improvements in adjuvant systemic treatment. Hence, optimization of surgical therapy is an overarching goal to improve patients’ outcomes. In this context, intraoperative tumor-specific imaging might facilitate optimized cytoreduction. In neurosurgery, intraoperative 5-aminolevulinic acid (5-ALA) guided imaging is applied in clinical routine to assess surgical resection margins. Here, we report the case of a patient with ovarian cancer in whom intraoperative 5-ALA tumor visualization led to optimized complete cytoreduction.ObjectiveIntraoperative administration of 5-ALA led to improved complete cytoreduction by identification and resection of additional ovarian cancer tumor manifestations.CaseThe 39-year-old patient, Jehovah`s witness, presented to our department with a left sided ovarian mass, suspicious of ovarian cancer, based on clinical examination, sonographic suspicious features and a CA12-5 elevation. The patient’s medical history and family history was unremarkable. Preoperative CT imaging of the thorax and abdomen showed no pathology besides the adnexal mass. Surgery was performed by a midline laparotomy with hysterectomy, bilateral adnexectomy, pelvic peritonectomy, omentectomy, ureterolysis, diaphragm stripping, adhesiolysis and the collection of peritoneal and rectal samples. Intraoperative 5-ALA imaging using a dedicated excitation and detection loupe system (Reveal, DVI) led to tumor detection at the diaphragm, the omentum and the rectum that was not detectable by palpation and visualization using white light. The pathology results revealed that the 5-ALA positive samples (diaphragm, rectum and omentum) obtained by intraoperative 5-ALA were positive for ovarian cancer.ConclusionIntraoperative administration of 5-ALA represents a promising approach to improve complete cytoreduction in ovarian cancer surgery thereby improving clinical outcomes. Hence, further research and clinical trials are required to investigate the potential of intraoperative 5-ALA imaging in ovarian cancer debulking surgery and its impact on long-term clinical outcomes.
Economic evaluation of breast MRI in screening - a systematic review and basic approach to cost-effectiveness analyses
Authors: Tollens Fabian , Baltzer Pascal A.T. , Froelich Matthias F. , Kaiser Clemens G. 
Published: December 2023

BackgroundEconomic evaluations have become an accepted methodology for decision makers to allocate resources in healthcare systems. Particularly in screening, where short-term costs are associated with long-term benefits, and adverse effects of screening intermingle, cost-effectiveness analyses provide a means to estimate the economic value of screening.PurposeTo introduce the methodology of economic evaluations and to review the existing evidence on cost-effectiveness of MR-based breast cancer screening.Materials and methodsThe various concepts and techniques of economic evaluations critical to the interpretation of cost-effectiveness analyses are briefly introduced. In a systematic review of the literature, economic evaluations from the years 2000-2022 are reviewed.ResultsDespite a considerable heterogeneity in the reported input variables, outcome categories and methodological approaches, cost-effectiveness analyses report favorably on the economic value of breast MRI screening for different risk groups, including both short- and long-term costs and outcomes.ConclusionEconomic evaluations indicate a strongly favorable economic value of breast MRI screening for women at high risk and for women with dense breast tissue.
Molecular cytogenetic identification and nutritional composition evaluation of newly synthesized Triticum turgidum-Triticum boeoticum amphiploids (AABBAbAb)
Authors: Liu Xin , Jiang Xiaomei , Zhang Junqing , Ye Hong , Shen Mang , Wu Lei , Miao Yongping , Chen Longyu , Zhou Ke , Hao Ming , Jiang Bo , Huang Lin , Ning Shunzong , Chen Xuejiao , Chen Xue , Liu Dengcai , Zhang Lianquan 
Published: December 2023

Triticum boeoticum Boiss. (AbAb, 2n = 2x = 14) is a wheat-related species with the blue aleurone trait. In this study, 18 synthetic Triticum turgidum-Triticum boeoticum amphiploids were identified, which were derived from crosses between T. boeoticum and T. turgidum. Three probes (Oligo-pTa535, Oligo-pSc119.2, and Oligo-pTa713) for multicolor fluorescence in situ hybridization (mc-FISH) were combined with genomic in situ hybridization (GISH) to identify chromosomal composition. Seven nutritional indices (anthocyanins, protein, total essential amino acids TEAA, Fe, Zn, Mn and Cu) were measured, and the nutritional components of 18 synthetic amphiploids were comprehensively ranked by principal component analysis (PCA). The results showed that all three synthetic amphiploids used for cytological identification contained 42 chromosomes, including 14 A, 14 B, and 14 Ab chromosomes. The average anthocyanin content was 82.830 μg/g to 207.606 μg/g in the whole meal of the 17 blue-grained lines (Syn-ABAb-1 to Syn-ABAb-17), which was obviously higher than that in the yellow-grained line Syn-ABAb-18 (6.346 μg/g). The crude protein content was between 154.406 and 180.517 g/kg, and the EAA content was 40.193-63.558 mg/g. The Fe, Zn, Mn and Cu levels in the 17 blue-grained lines were 60.55 to 97.41 mg/kg, 60.55-97.41 mg/kg, 35.11 to 65.20 mg/kg and 5.74 to 7.22 mg/kg, respectively, which were higher than those in the yellow-grained line. The contribution of the first three principal components reached 84%. The first principal component was mainly anthocyanins, Fe, Zn and Mn. The second principal component contained protein and amino acids, and the third component contained only Cu. The top 5 Triticum turgidum-Triticum boeoticum amphiploids were Syn-ABAb-11, Syn-ABAb-17, Syn-ABAb-5, Syn-ABAb-8 and Syn-ABAb-4. These amphidiploids exhibited the potential to serve as candidates for hybridization with common wheat, as indicated by comprehensive score rankings, toward enhancing the nutritional quality of wheat.
Athlete anxiety questionnaire: the development and validation of a new questionnaire for assessing the anxiety, concentration and self-confidence of athletes
Authors: Trpkovici Melinda , Pálvölgyi Ágnes , Makai Alexandra , Prémusz Viktória , Ács Pongrác 
Published: December 2023

IntroductionAnxiety is one of the most prevalent issues among athletes. Therefore, measuring the stress caused by high-stakes situations could be important for investigating the issue. In sports psychology literature, no valid and reliable questionnaire is available in Hungarian for assessing the anxiety experienced by athletes in high-stakes situations. This study aimed to create a new Hungarian questionnaire to measure anxiety, self-confidence, and concentration during high-stakes contests.Methods263 athletes of various sports participated in the cross-sectional study (age: 16.18 ± 3.46 years). The structure of the Anxiety Athletes Questionnaire (AAQ) was examined through factor analysis, where exploratory factor analysis (EFA) as well as confirmatory factor analysis (CFA) were carried out. The internal consistency of the subscales of AAQ was measured by Cronbach’s alpha. Through a convergent validity test, the AAQ questionnaire was compared to the subscales of the CSAI-2 and ACSI-28 questionnaire subscales by Spearman’s rank correlation coefficients. Through a discriminant validity analysis, the differences by age group, sex, and sport variables were examined by AAQ scores. The Mann–Whitney U and Kruskal-Wallis H tests were utilized in the analysis. The SPSS 28.0 software was used for the statistical analysis, and the level of significance was set at p &lt; 0.05.ResultsFour factors have been identified through the EFA. The CFA analysis showed the four-factor model an acceptable model fit (SRMR, RMSEA CFI, TLI). Cronbach’s alpha of the four subscales showed acceptable internal consistency (cognitive anxiety: α = 0.871; somatic anxiety: α = 0.700; self-confidence: α = 0.832; concentration: α = 0.747). The convergent validity showed a weak or moderate, significant relationship between AAQ subscales and subscales of CSAI and ACSI (R = −0.398–0.412).ConclusionThe Athlete Anxiety Questionnaire can be considered a reliable and valid measurement tool for measuring athletes’ anxiety, self-confidence and concentration in high-stakes situations.