bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional partitioning of sentence processing and emotional prosody in the right perisylvian cortex after perinatal stroke
Authors: Martin Kelly C. , Seydell-Greenwald Anna , Turkeltaub Peter E. , Chambers Catherine E. , Gaillard William D. , Newport Elissa L. 
Published: February 2024

Abstract

In healthy adults different language abilities—sentence processing versus emotional prosody—are supported by the left (LH) versus the right hemisphere (RH), respectively. However, after LH stroke in infancy, RH regions support both abilities with normal outcomes. We investigated how these abilities co-exist in RH regions after LH perinatal stroke by evaluating the overlap in the activation between two fMRI tasks that probed auditory sentence processing and emotional prosody processing. We compared the overlap for these two functions in the RH of individuals with perinatal stroke with the symmetry of these functions in the LH and RH of their healthy siblings. We found less activation overlap in the RH of individuals with LH perinatal stroke than would be expected if both functions retained their typical spatial layout, suggesting that their spatial segregation may be an important feature of a functioning language system.
Multiomics approaches disclose very-early molecular and cellular switches during insect-venom allergen-specific immunotherapy
Authors: Hefeng Feng , Pogorelov Dimitrii , Bode Sebastian , He Xin , Ramiro-Garcia Javier , Hedin Fanny , Ammerlaan Wim , Konstantinou Maria , Capelle Christophe , Zeng Ni , Poli Aurélie , Domingues Olivia , Montamat Guillem , Hunewald Oliver , Cire Séverine , Baron Alexandre , Longworth Joseph , Neuberger-Castillo Lorie , Revets Dominique , Guyonnet Léa , Demczuk Agnieszka , Delhalle Sylvie , Zimmer Jacques , Benes Vladimir , Codreanu-Morel Françoise , Lehners-Weber Christiane , Weets Ilse , Alper Pinar , Brenner Dirk , Gutermuth Jan , Guérin Coralie , Morisset Martine , Hentges François , Schneider Reinhard , Shamji Mohamed , Betsou Fay , Wilmes Paul , Glaab Enrico , Goncalves Jorge , Cosma Antonio , Ollert Markus 
Published: February 2024

Abstract

Allergen-specific immunotherapy (AIT) induces immune tolerance, showing the highest success rate (>95%) for insect venom while a much lower chance for pollen allergy. However, the molecular switches leading to successful durable tolerance restoration remain elusive. Here we applied a multilayer-omics approach to reveal dynamic peripheral immune landscapes during AIT-initiation phase in venom allergy patients (VAP) versus pollen-allergic and healthy controls. Already at baseline, VAP exhibited altered abundances of several cell types, including antigen-presenting cells (APC) and hybrid types, especially pDC-mDC hybrids. At 8-24h following AIT launch in VAP, we identified a uniform AIT-elicited pulse of late-transitional/IL-10-producing B cells, IL-6 signaling within Th2 cells and non-inflammatory serum-IL-6 levels. Sequential induction of activation and survival protein markers also immediately occurred. A disequilibrium between serum IL-6 and APC in VAP baseline was restored at day seven following AIT launch. Our longitudinal analysis discovers molecular switches during initiation-phase insect-venom AIT that secure long-term outcomes.
Dipyridamole Reduces Lung Injury in a Rat Lung Ischemia and Reperfusion Model
Authors: Özgür Yücel , Altun Şenel , Işık Reyhan , Kılıçkaya Refika , Özcan Burcu , Şahin Nurettin , Köksal Bülent 
Published: February 2024

Abstract

Background: In this study, the effect of Dipyridamole (DIP) on lung tissue in lung ischemia/reperfusion (I/R) injury in rats was investigated.
Methods: A total of 24 Wistar rats were divided into four equal groups with six rats in each group: Group Sham, Group I/R (ischemia/reperfusion), and Group I/R+ 10mg/kg DIP, Group I/R+ 100mg/kg DIP. 45 minutes of ischemia and 120 minutes of reperfusion were applied.
Results: TNF-α value was decreased in Groups 10mg/kg DIP and I/R+ 100mg/kg DIP compared to Group I/R. TNF-α value value was lower in Groups 10mg/kg DIP and 100mg/kg DIP. The difference in TNF-α value was statistically significant between the groups (p=0.002). Statistically significant results were observed between Group I/R-DIP 100 mg/kg and Group I/R for TNF-α. (p=0.038).
MDA value in the blood was higher in Group I/R than in Group Sham. MDA value was decreased in Groups 10mg/kg DIP and 100mg/kg DIP compared to Group I/R. However, the difference in MDA value was not statistically significant between the groups (p=0.071). Catalase level in the blood was higher in Group I/R than Group Sham.). The difference in CAT activity was statistically significant between the groups (p=0.025). Statistically significant results were observed between Group I/R-DIP 100 and Group I/R for CAT. (p=0.047).
According to histopathological examination, lung tissue neutrophil infiltration, pulmonary edema, interstitial edema, alveolar hemorrhage and total score levels were significantly higher in Group I/R than Groups I/R-10mg/kg DIP and I/R-100mg/kg DIP(p=0.02, p=0.03, p=0.01, respectively, p=0.03 and p=0.01). Lung tissue pulmonary edema, interstitial edema, neutrophil infiltration, alveolar hemorrhage and total score levels were significantly lower in Group 10mg/kg DIP compared to Group I/R (p=0.03, p=0.01, p=0.02, p=0.03 and p=0.01, respectively). ). Lung tissue pulmonary edema, interstitial edema, neutrophil infiltration, alveolar hemorrhage and total score levels were significantly lower in Group I/R-100mg/kg DIP compared to Group I/R (p=0.009, p=0.002, p=0.001, p=0.002 and p=0.002, respectively). ).
Conclusion: Lung tissue may be affected by ischemia/reperfusion injury, and this damage can be reversed with the use of dipyridamole.
Astrocyte morphogenesis requires self-recognition
Authors: Zipursky S. , Lee John , Sergeeva Alina , Ahlsen Goran , Mannepalli Seetha , Bahna Fabiana , Goodman Kerry , Khakh Baljit , Weiner Joshua , Shapiro Lawrence , Honig Barry 
Published: February 2024

Abstract
Self-recognition is a fundamental cellular process across evolution and forms the basis of neuronal self-avoidance1-4. Clustered protocadherins (Pcdh), comprising a large family of isoform-specific homophilic recognition molecules, play a pivotal role in neuronal self-avoidance required for mammalian brain development5-7. The probabilistic expression of different Pcdh isoforms confers unique identities upon neurons and forms the basis for neuronal processes to discriminate between self and non-self5,6,8. Whether this self-recognition mechanism exists in astrocytes, the other predominant cell type of the brain, remains unknown. Here, we report that a specific isoform in the Pcdhγ cluster, γC3, is highly enriched in human and murine astrocytes. Through genetic manipulation, we demonstrate that γC3 acts autonomously to regulate astrocyte morphogenesis in the mouse visual cortex. To determine if γC3 proteins act by promoting recognition between processes of the same astrocyte, we generated pairs of γC3 chimeric proteins capable of heterophilic binding to each other, but incapable of homophilic binding. Co-expressing complementary heterophilic binding isoform pairs in the same γC3 null astrocyte restored normal morphology. By contrast, chimeric γC3 proteins individually expressed in single γC3 null mutant astrocytes did not. These data establish that self-recognition is essential for astrocyte development in the mammalian brain and that, by contrast to neuronal self-recognition, a single Pcdh isoform is both necessary and sufficient for this process.
Transparent and High-porosity Aluminum Alkoxide Network-forming Glasses
Authors: Zhao Yingbo , Zhang Zihui 
Published: February 2024

Abstract

Metal-organic network-forming glasses are an emerging type of material capable of combining the modular design and high porosity of metal-organic frameworks and the high processability and optical transparency of glasses. However, a generalizable strategy for achieving both high porosity and high glass forming ability in modularly designed metal-organic networks has yet to be developed. Herein, we developed a new series of metal-organic network-forming glasses, aluminum alkoxide glasses, by linking aluminum-oxo clusters with alcohol linkers in the presence of a modulator template. These glasses exhibit well-defined glass transitions and high surface areas up to 500 m2/g, making them one of the most porous glassy materials. The aluminum alkoxide glasses also have optical transparency and fluorescent properties, and their structures were elucidated by pair-distribution functions and compositional analysis. A systematic glass transition study suggested that progressive increase in network connectivity during the evaporation of a coordinatively competitive solvent is key to the bottom-up glass synthesis. Aluminum alkoxide glass can also encapsulate crystalline MOFs to yield composite materials with higher porosities. These findings could significantly expand the library of microporous metal-organic network-forming glasses and enable their future applications.
Study on hydraulic fracture propagation of strong heterogeneous shale based on stress-seepage damage coupling model
Authors: Liu Wei , Xiao Lei , Ke Yunbin , Dong Kangxing 
Published: February 2024

Abstract

The developed laminar structure of shale makes it have obvious anisotropic characteristics, and the anisotropic characteristics are one of the important factors leading to the strong and weak heterogeneous properties of shale, and there is a common phenomenon that the propagation of hydraulic fracture heights is limited in the hydraulic fracturing process of strong heterogeneous shale reservoirs. In order to clarify the reasons for the limited height propagation of hydraulic fractures in strongly heterogeneous shale reservoirs, numerical simulation methods were used to study the propagation patterns of hydraulic fractures in shale reservoirs under different anisotropy, flow rate, viscosity, and stress differences. The results show that as the anisotropy value increases and the heterogeneity becomes stronger, the more difficult it is for hydraulic fractures to expand along the fracture height direction, and the larger the fracture width is at the fracture opening. For the strong heterogeneous shale reservoir, the hydraulic fracture height can be increased within a certain range by increasing the flow rate, increasing the viscosity and increasing the stress difference. When the upper limit value is exceeded, the hydraulic fracture height of the strong heterogeneous shale reservoir gradually becomes stable, and the increasing trend is no longer obvious. The strong or weak heterogeneity caused by the inherent anisotropic characteristics of shale reservoirs will affect the development and transformation effectiveness of shale reservoirs. The research in this article will provide some reference and guidance for the efficient development of shale reservoirs.
Hallmarks of a Genomically Distinct Subclass of Head and Neck Cancer
Authors: Muijlwijk Tara , Nauta Irene , Lee Anabel van der , Grünewald Kari , Brink Arjen , Ganzevles Sonja , de Jong Robert Baatenburg , Atanesyan Lilit , s.savola@mrcholland.com Suvi , de Wiel Mark van , Peferoen Laura , Bloemena Elisabeth , de Ven Rieneke van , Leemans C. , Poell Jos , Brakenhoff Ruud 
Published: February 2024

Abstract

Cancer is caused by an accumulation of somatic mutations and copy number alterations (CNAs). Besides mutations, these copy number changes are key characteristics of cancer development, but nonetheless some tumors show hardly any CNAs, a remarkable phenomenon in oncogenesis. Head and neck squamous cell carcinomas (HNSCCs) arise by either exposure to carcinogens, or infection with the human papillomavirus (HPV). HPV-negative HNSCCs are generally characterized by many CNAs and frequent mutations in CDKN2A, TP53, FAT1 and NOTCH1. Here we present the hallmarks of the distinct subgroup of HPV-negative HNSCC with no or few CNAs (CNA-quiet) by genetic profiling of 802 oral squamous cell carcinomas (OCSCCs). In total, 73 OCSCC (9.1%) were classified as CNA-quiet and 729 as CNA-other. The CNA-quiet group was characterized by wild-type TP53, frequent CASP8 and HRAS mutations, and a less immunosuppressed tumor immune microenvironment with lower density of regulatory T cells. Patients with CNA-quiet OCSCC were older, more often women, less frequently current smokers and had a better 5-year overall survival compared to CNA-other OCSCC. This study demonstrates that CNA-quiet OCSCC should be considered as a distinct, clinically relevant subclass. Given the clinical characteristics, the patient group with these tumors will rapidly increase in the aging population.
Response surface approach of Propylene Glycol and Water (75:25)/Graphene+MWCNT nanofluid viscosity
Authors: Surakasi Raviteja , Manyala Gangadhar Rao , Ch Polayya 
Published: February 2024

Abstract
Response surface methodology (RSM) was used to study a Propylene Glycol-water nanofluid. The nano powder content was varied to make three PG + W (75:25) samples. Graphene and MWCNT nano particles were blended in base fluids at 0.25% and 0.5% using four-ball milling. Temperature decreases viscosity, and the combination PG + W (75:25) + 0.5% G + MWCNT has the lowest viscosity at 0.32 m2/s. The laboratory parameters in this study include a 40–120 ◦C temperature range and a 0 to 0.5% weight percentage. Finding the best prediction model and solution is the goal. The statistical-mathematical investigation of modelers' performance, contrast, and motives and the inconsistency between laboratory behaviour and real-world applications prompted this study. Some tested models are 2FI, quadratic, cubic, and quartic. Analysis of these modelling functions' statistical properties is a novel contribution to the area. Statistical research shows that the cubic model depicts nanofluids twice as accurately as other models. The R2 coefficient, C.V%, and P-value are used to evaluate models. The cubic model indices are 0.9936, 3.54%, and 0.0001. At 109.661 C, nanofluids should have 0.401 m2/sec viscosity and 0.278 weight percentage.
Associations of Helicobacter Pylori with Anemia: A Bidirectional Two-Sample Mendelian Randomization Study
Authors: Xia Chuqi , Lei Wanyang , Yang Yongyu , Sun Guowu , Zhang Yang , Li Xinyue , Xu Ning , Liang Daoming 
Published: February 2024

Abstract
Background
Observational studies have reported a correlation between Helicobacter pylori infection and an increased incidence of anemia, iron deficiency anemia (IDA), and vitamin B12 deficiency anemia. However, the fundamental cause remains elusive. This study aimed to ascertain the causal relationship between H. pylori infection and anemia.
Method
Using data from genome-wide association studies (GWAS), we executed a bidirectional Mendelian randomization (MR) study utilizing two-sample GWAS. The principal analytical method was the inverse variance weighted (IVW) method. We employed a conservative Bonferroni-corrected threshold (p < 2.38 × 10− 3). Sensitivity analyses were implemented with Cochran’s Q test and MR-Egger regression.
Results
Genetically predicted H. pylori infection, anti-H. pylori CagA, VacA, and IgG seropositivity was not causally associated with anemia, IDA, or vitamin B12 deficiency anemia (p > 2.38 × 10− 3). Furthermore, reverse MR analysis did not reveal evidence for a causal effect of anemia on H. pylori infection (p > 0.05).
Conclusions
Utilizing genetic data, MR analysis demonstrated the absence of evidence for a causal association between the seroprevalence of H. pylori infection and anemia. Further research is needed to elucidate the relationship between H. pylori infection and anemia.
Streamline topologies and bifurcation analysis for electroosmosis peristaltic flow of FENE-P fluid through an asymmetric channel
Authors: Ullah Kaleem , Shahzad Hasan , Algarni Salem , Alqahtani Talal , Irshad Kashif 
Published: February 2024

Abstract

The bifurcation of stagnation points and streamlines topologies of various flow phenomena for the transportation of FENE-P fluid due to electro-osmotic peristaltic activity have been investigated via dynamical system approach. The study focuses on the flow within an asymmetric channel, using well-known approximations that assume long wavelength and an extremely low Reynolds number. Autonomous differential equations have been established to point out the stagnation points. Three various flow distributions: backward, trapping and augmented flow are cropped up in the flow. The transition of these phenomena correspond two critical (bifurcation) conditions. Variations of the involved parameters have a notable impact on the streamline topologies in these flow distributions, as well as on the bifurcations of stagnation points. The trapping region is found to expand by enlarging the electric potential on the channel walls. However, increments in the Helmholtz-Smoluchowski velocity and electro-osmotic parameter reduces the trapping phenomenon in the flow. The findings acquired undergo examination through global bifurcation diagrams. This analysis could form the basis for constructing a mathematical framework. Such a framework would be useful in investigating the quantitative influences of the parameters involved in the diverse flow phenomena.
Real-World Effectiveness of First-Line Azacitidine or Decitabine with or without Venetoclax in AML Patients Unfit for Intensive Therapy
Authors: Acker Fabian , Chromik Jörg , Tiedjen Emily , Wolf Sebastian , Vischedyk Jonas B. , Makowka Philipp , Enßle Julius C. , Kouidri Khouloud , Sebastian Martin , Steffen Björn , Oellerich Thomas , Serve Hubert , Neubauer Andreas , Schäfer Jonas A. , Bittenbring Jörg T. 
Published: February 2024

Abstract

Standard frontline treatment in patients with acute myeloid leukemia (AML) unfit for intensive therapy is the combination of a hypomethylating agent (HMA) with venetoclax (VEN). However, retrospective data confirming the benefits of this regimen outside of clinical trials are sparse and have shown conflicting results. Thus, we performed a multicenter retrospective analysis of outcomes with HMA-VEN compared to HMA alone in patients with newly diagnosed AML unfit for intensive treatment. A total of 213 patients were identified from 3 German tertiary care centers. Of those, 125 were treated with HMA-VEN and 88 with HMA alone. Median overall survival (OS) in the HMA-VEN cohort was 7.9 months (95% confidence interval [CI], 5.1–14.7) compared to 4.9 months (3.1–7.1) with HMA alone. After 1 year, 42% (95% CI, 33–54) and 19% (12–30) of patients were alive, respectively. The hazard ratio (HR) for death was 0.64 (95% CI, 0.46–0.88; p = 0.006). After adjusting for age, NCCN cytogenetic risk, NPM1, RUNX1, and TP53 status, ECOG performance status, baseline leukocytes, and type of HMA, treatment with HMA-VEN remained significantly associated with a prolonged survival (HR, 0.48; 95% CI, 0.29–0.77). Accordingly, time to next treatment (TTNT) was longer with HMA-VEN with a HR of 0.63 (95% CI, 0.47–0.85). Patients who achieved recovery of peripheral blood counts had a favorable prognosis (HR for death, 0.52; 95% CI, 0.33–0.84). These data align with findings from the pivotal VIALE-A trial and support the use of HMA-VEN in patients unfit for intensive therapy.
Serum cytokines and creatinine/cystatin C ratio as prognostic biomarkers in advanced cancer patients treated with anti-PD-1/PD-L1 therapy
Authors: Jin Shan-xiu , Liu Bo-Na , Ji juan , Wu Jing-ran , Li Bao-lei , Gao Xiao-li , Li Na , Zheng Zhen-dong , Du Cheng 
Published: February 2024

Abstract

Objective
Immune checkpoint inhibitors (ICIs), specifically targeting the programmed cell death protein-1 or its ligand (PD-1/PDL-1), have been extensively used in the treatment of a spectrum of malignancies, although the predictive biomarkers remain to be elucidated. This study aims to investigate the association between baseline circulating levels of cytokines and the creatinine/cystatin C ratio (CCR) with the treatment outcomes of ICIs in patients with advanced cancer.
Methods
The pre-treatment circulating levels of 10 cytokines (PD-L1, CTLA4, CXCL10, LAG3, HGF, CCL2, MIG, GRANB, IL-18, and IL-6) were measured via automated capillary-based immunoassay platform in the serum of 65 advanced cancer patients treated with anti-PD-1/PD-L1-based systemic therapy and 10 healthy volunteers. The levels of cytokines and CCR were quantified and categorized into high and low groups based on the median value. The associations of serum cytokines and CCR with response to treatment, survival, and immune-related adverse events were assessed.
Results
Elevated circulating levels of 6 cytokines (PD-L1, CXCL10, HGF, CCL2, MIG, and IL-6) were observed in cancer patients compared with that in healthy volunteers. In the cancer cohort (N = 65), low circulating HGF (P = 0.023, P = 0.029), low IL-6 (P = 0.002, P < 0.001), and high CCR (P = 0.031, P = 0.008) were associated with significantly improved progression-free survival (PFS) and overall survival (OS). Multi-variable COX analyses adjusted for clinicopathological factors revealed that low HGF, low IL-6, and high CCR were independent favorable prognostic factors for PFS (P = 0.028, P = 0.010, and P = 0.015, respectively) and OS (P = 0.043, P = 0.003, and P = 0.026, respectively). Grade 2 irAEs occurred more frequently in patients with low levels of circulating CCL2 and LAG3.
Conclusions
Pre-treatment circulating levels of serum IL-6, HGF, and CCR may serve as independent predictive and prognostic biomarkers in advanced cancer patients treated with ICIs-based systemic therapy. These findings might help to identify potential patients who would benefit from these therapies.
Characterization of antibiotic resistomes and pathobiomes of soil antibiotic-degrading microbial by stable isotope probing and metagenomics
Authors: Wang Fang , Mei Zhi , He Chao , Fu Yuhao , Virta Marko , Liao Maoyuan , Hashsham Syed , Jiang Xin , Jia Zhongjun , Zhang Tong , Tiedje James 
Published: February 2024

Abstract

The presence and spread of antimicrobial resistance genes (ARGs) in soil represent a significant threat to human health. However, the ARGs harbored by antibiotic-degrading microorganisms in soil are inadequately characterized. Employing diverse molecular techniques, we investigate the dynamics of ARGs, mobile genetic elements, and virulence factors in soils contaminated with the antibiotic 13C-sulfadiazine (SDZ), enabling the identification of active SDZ-degrading bacteria. We find that the addition of biochar-based biofilm to various soil types reduces the abundance of several ARGs and virulence factors associated with active SDZ-degrading bacteria. Furthermore, our results reveal a marked increase in the relative abundance of specific genes within associated microorganisms during SDZ metabolism in the soil. This study elucidates the distribution and composition of antimicrobial resistance in active SDZ-degrading bacteria across diverse soils, suggesting that the utilization of biochar-based biofilm represents an effective strategy for mitigating antimicrobial resistance in soil within the One Health framework.