bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hyperspectral and Photodiode Retrievals of Nighttime LED‐Induced Chlorophyll Fluorescence (LEDIF) for Tracking Photosynthetic Phenology in a Vineyard
Authors: Wong Christopher Y. S. , McHugh Devin P. , Bambach Nicolas , McElrone Andrew J. , Alsina Maria Mar , Kustas William P. , Magney Troy S. 
Published: January 2024

AbstractThe magnitude of chlorophyll fluorescence emission represents both chlorophyll content and energy quenching processes enabling its application to serve as a proxy of photosynthetic activity. Thus, there is interest in advancing methods for canopy‐scale monitoring of chlorophyll fluorescence. Remotely sensed solar‐induced fluorescence (SIF) retrievals offer daytime monitoring of chlorophyll fluorescence, which can serve as an indicator of photosynthesis. However, it represents an instantaneous measurement during the day, which is strongly influenced by incoming radiation, solar angle, and sun/shade fraction—making it difficult to tease out baseline information on plant health and potential photosynthetic capacity—which could be tracked by changes in fluorescence yield (independent of sunlight). Recent advances have demonstrated the potential for inducing nighttime chlorophyll fluorescence via LED light sources at the canopy‐scale, which can be retrieved as LED‐induced chlorophyll fluorescence (LEDIF), potentially serving as a baseline indicator of plant health and photochemical capacity, independent of daytime conditions. In this study, we explored two methods of LEDIF retrievals: (a) hyperspectral sensor (1.33 nm full‐width half max) and (b) low‐cost Red‐Far‐Red photodiode sensor. LEDIF retrieved by the hyperspectral sensor demonstrated strong correlations with daytime SIF and gross primary productivity during mid to end of season phenology (R2 > 0.70). In contrast, phenological dynamics of LEDIF retrieved by the photodiode sensor was more subtle, likely due to weaker signal‐to‐noise ratio, but still demonstrated some potential. Overall, LEDIF offers a technique to monitor nighttime chlorophyll fluorescence emissions (and changes in its spectral shape with a hyperspectral sensor) to assess canopy‐scale phenology of photosynthetic potential.
The multifaceted functions of β-arrestins and their therapeutic potential in neurodegenerative diseases
Authors: Kee Teresa R. , Khan Sophia A. , Neidhart Maya B. , Masters Brianna M. , Zhao Victoria K. , Kim Yenna K. , McGill Percy Kyle C. , Woo Jung-A A. 
Published: January 2024

AbstractArrestins are multifunctional proteins that regulate G-protein-coupled receptor (GPCR) desensitization, signaling, and internalization. The arrestin family consists of four subtypes: visual arrestin1, β-arrestin1, β-arrestin2, and visual arrestin-4. Recent studies have revealed the multifunctional roles of β-arrestins beyond GPCR signaling, including scaffolding and adapter functions, and physically interacting with non-GPCR receptors. Increasing evidence suggests that β-arrestins are involved in the pathogenesis of a variety of neurodegenerative diseases, including Alzheimer’s disease (AD), frontotemporal dementia (FTD), and Parkinson’s disease (PD). β-arrestins physically interact with γ-secretase, leading to increased production and accumulation of amyloid-beta in AD. Furthermore, β-arrestin oligomers inhibit the autophagy cargo receptor p62/SQSTM1, resulting in tau accumulation and aggregation in FTD. In PD, β-arrestins are upregulated in postmortem brain tissue and an MPTP model, and the β2AR regulatesSNCAgene expression. In this review, we aim to provide an overview of β-arrestin1 and β-arrestin2, and describe their physiological functions and roles in neurodegenerative diseases. The multifaceted roles of β-arrestins and their involvement in neurodegenerative diseases suggest that they may serve as promising therapeutic targets.
Apically localized PANX1 impacts neuroepithelial expansion in human cerebral organoids
Authors: Noort Rebecca J. , Zhu Hanrui , Flemmer Robert T. , Moore Craig S. , Belbin Thomas J. , Esseltine Jessica L. 
Published: January 2024

AbstractDysfunctional paracrine signaling through Pannexin 1 (PANX1) channels is linked to several adult neurological pathologies and emerging evidence suggests that PANX1 plays an important role in human brain development. It remains unclear how early PANX1 influences brain development, or how loss of PANX1 alters the developing human brain. Using a cerebral organoid model of early human brain development, we find that PANX1 is expressed at all stages of organoid development from neural induction through to neuroepithelial expansion and maturation. Interestingly, PANX1 cellular distribution and subcellular localization changes dramatically throughout cerebral organoid development. During neural induction, PANX1 becomes concentrated at the apical membrane domain of neural rosettes where it co-localizes with several apical membrane adhesion molecules. During neuroepithelial expansion, PANX1−/− organoids are significantly smaller than control and exhibit significant gene expression changes related to cell adhesion, WNT signaling and non-coding RNAs. As cerebral organoids mature, PANX1 expression is significantly upregulated and is primarily localized to neuronal populations outside of the ventricular-like zones. Ultimately, PANX1 protein can be detected in all layers of a 21–22 post conception week human fetal cerebral cortex. Together, these results show that PANX1 is dynamically expressed by numerous cell types throughout embryonic and early fetal stages of human corticogenesis and loss of PANX1 compromises neuroepithelial expansion due to dysregulation of cell-cell and cell-matrix adhesion, perturbed intracellular signaling, and changes to gene regulation.
ZO-1 regulates the migration of mesenchymal stem cells in cooperation with α-catenin in response to breast tumor cells
Authors: Park Aran , Choi Sanghyuk , Do Jungbeom , Kim Youngjae , Kim Kyung-Sup , Koh Eunjin , Park Ki-Sook 
Published: January 2024

AbstractsMesenchymal stem cells are recruited from the bone marrow into breast tumors, contributing to the creation of a tumor microenvironment that fosters tropism for breast tumors. However, the intrinsic mechanisms underlying the recruitment of bone marrow-derived mesenchymal stem cells (MSCs) into the breast tumor microenvironment are still under investigation. Our discoveries identified zonula occludens-1 (ZO-1) as a specific intrinsic molecule that plays a vital role in mediating the collective migration of MSCs towards breast tumor cells and transforming growth factor beta (TGF-β), which is a crucial factor secreted by breast tumor cells. Upon migration in response to MDA-MB-231 cells and TGF-β, MSCs showed increased formation of adherens junction-like structures (AJs) expressing N-cadherin and α-catenin at their cell-cell contacts. ZO-1 was found to be recruited into the AJs at the cell-cell contacts between MSCs. Additionally, ZO-1 collaborated with α-catenin to regulate AJ formation, dependently on the SH3 and GUK domains of the ZO-1 protein. ZO-1 knockdown led to the impaired migration of MSCs in response to the stimuli and subsequent downregulation of AJs formation at the cell-cell contacts during MSCs migration. Overall, our study highlights the novel role of ZO-1 in guiding MSC migration towards breast tumor cells, suggesting its potential as a new strategy for controlling and re-engineering the breast tumor microenvironment.
Role of Sox9 in BPD and its effects on the Wnt/β-catenin pathway and AEC-II differentiation
Authors: Wu Di , Bai Dongqin , Yang Miao , Wu Bo , Xu Wei 
Published: January 2024

AbstractThe excessive activation of the Wnt/β-catenin signaling pathway is an important regulatory mechanism that underlies the excessive proliferation and impaired differentiation of type 2 alveolar epithelial cells (AEC-II) in bronchopulmonary dysplasia (BPD). Sox9 has been shown to be an important repressor of the Wnt/β-catenin signaling pathway and plays an important regulatory role in various pathophysiological processes. We found that the increased expression of Sox9 in the early stages of BPD could downregulate the expression of β-catenin and promote the differentiation of AEC-II cells into AEC-I, thereby alleviating the pathological changes in BPD. The expression of Sox9 in BPD is regulated by long noncoding RNA growth arrest-specific 5. These findings may provide new targets for the early intervention of BPD.
A CYBDOM protein impacts iron homeostasis and primary root growth under phosphate deficiency in Arabidopsis
Authors: Clúa Joaquín , Montpetit Jonatan , Jimenez-Sandoval Pedro , Naumann Christin , Santiago Julia , Poirier Yves 
Published: January 2024

AbstractArabidopsis primary root growth response to phosphate (Pi) deficiency is mainly controlled by changes in apoplastic iron (Fe). Upon Pi deficiency, apoplastic Fe deposition in the root apical meristem activates pathways leading to the arrest of meristem maintenance and inhibition of cell elongation. Here, we report that a member of the uncharacterized cytochrome b561 and DOMON domain (CYBDOM) protein family, named CRR, promotes iron reduction in an ascorbate-dependent manner and controls apoplastic iron deposition. Under low Pi, the crr mutant shows an enhanced reduction of primary root growth associated with increased apoplastic Fe in the root meristem and a reduction in meristematic cell division. Conversely, CRR overexpression abolishes apoplastic Fe deposition rendering primary root growth insensitive to low Pi. The crr single mutant and crr hyp1 double mutant, harboring a null allele in another member of the CYDOM family, shows increased tolerance to high-Fe stress upon germination and seedling growth. Conversely, CRR overexpression is associated with increased uptake and translocation of Fe to the shoot and results in plants highly sensitive to Fe excess. Our results identify a ferric reductase implicated in Fe homeostasis and developmental responses to abiotic stress, and reveal a biological role for CYBDOM proteins in plants.
OXR1 maintains the retromer to delay brain aging under dietary restriction
Authors: Wilson Kenneth A. , Bar Sudipta , Dammer Eric B. , Carrera Enrique M. , Hodge Brian A. , Hilsabeck Tyler A. U. , Bons Joanna , Brownridge George W. , Beck Jennifer N. , Rose Jacob , Granath-Panelo Melia , Nelson Christopher S. , Qi Grace , Gerencser Akos A. , Lan Jianfeng , Afenjar Alexandra , Chawla Geetanjali , Brem Rachel B. , Campeau Philippe M. , Bellen Hugo J. , Schilling Birgit , Seyfried Nicholas T. , Ellerby Lisa M. , Kapahi Pankaj 
Published: January 2024

AbstractDietary restriction (DR) delays aging, but the mechanism remains unclear. We identified polymorphisms in mtd, the fly homolog of OXR1, which influenced lifespan and mtd expression in response to DR. Knockdown in adulthood inhibited DR-mediated lifespan extension in female flies. We found that mtd/OXR1 expression declines with age and it interacts with the retromer, which regulates trafficking of proteins and lipids. Loss of mtd/OXR1 destabilized the retromer, causing improper protein trafficking and endolysosomal defects. Overexpression of retromer genes or pharmacological restabilization with R55 rescued lifespan and neurodegeneration in mtd-deficient flies and endolysosomal defects in fibroblasts from patients with lethal loss-of-function of OXR1 variants. Multi-omic analyses in flies and humans showed that decreased Mtd/OXR1 is associated with aging and neurological diseases. mtd/OXR1 overexpression rescued age-related visual decline and tauopathy in a fly model. Hence, OXR1 plays a conserved role in preserving retromer function and is critical for neuronal health and longevity.
The HER2-directed antibody-drug conjugate DHES0815A in advanced and/or metastatic breast cancer: preclinical characterization and phase 1 trial results
Authors: Lewis Gail D. , Li Guangmin , Guo Jun , Yu Shang-Fan , Fields Carter T. , Lee Genee , Zhang Donglu , Dragovich Peter S. , Pillow Thomas , Wei BinQing , Sadowsky Jack , Leipold Douglas , Wilson Tim , Kamath Amrita , Mamounas Michael , Lee M. Violet , Saad Ola , Choeurng Voleak , Ungewickell Alexander , Monemi Sharareh , Crocker Lisa , Kalinsky Kevin , Modi Shanu , Jung Kyung Hae , Hamilton Erika , LoRusso Patricia , Krop Ian , Schutten Melissa M. , Commerford Renee , Sliwkowski Mark X. , Cho Eunpi 
Published: January 2024

AbstractApproved antibody-drug conjugates (ADCs) for HER2-positive breast cancer include trastuzumab emtansine and trastuzumab deruxtecan. To develop a differentiated HER2 ADC, we chose an antibody that does not compete with trastuzumab or pertuzumab for binding, conjugated to a reduced potency PBD (pyrrolobenzodiazepine) dimer payload. PBDs are potent cytotoxic agents that alkylate and cross-link DNA. In our study, the PBD dimer is modified to alkylate, but not cross-link DNA. This HER2 ADC, DHES0815A, demonstrates in vivo efficacy in models of HER2-positive and HER2-low cancers and is well-tolerated in cynomolgus monkey safety studies. Mechanisms of action include induction of DNA damage and apoptosis, activity in non-dividing cells, and bystander activity. A dose-escalation study (ClinicalTrials.gov: NCT03451162) in patients with HER2-positive metastatic breast cancer, with the primary objective of evaluating the safety and tolerability of DHES0815A and secondary objectives of characterizing the pharmacokinetics, objective response rate, duration of response, and formation of anti-DHES0815A antibodies, is reported herein. Despite early signs of anti-tumor activity, patients at higher doses develop persistent, non-resolvable dermal, ocular, and pulmonary toxicities, which led to early termination of the phase 1 trial.
Neuro-ophthalmological changes in healthy females exposed to a 5-day dry immersion: a pilot study
Authors: Kermorgant Marc , Chedmail Thibault , Varenne Fanny , Bareille Marie-Pierre , Beck Arnaud , Billette de Villemeur Rebecca , Fournié Pierre , Grondin Lisa , Hélissen Ophélie , Membrives Charlotte , Nasr Nathalie , Pavy-Le Traon Anne , Soler Vincent 
Published: January 2024

AbstractAfter exposure to microgravity, astronauts undergo microgravity-induced thoraco-cephalic fluid shift, which may lead to ocular changes called “spaceflight associated neuro-ocular syndrome” (SANS). The onset of SANS may be multifactorial, including a potential elevation in intracranial pressure. Moreover, little is known about the impact of spaceflight on SANS in women due to the fact that fewer female astronauts have spent time in long-term missions. The objective is to determine whether similar ophthalmological changes occur in healthy women after short-term exposure to microgravity. The auto-refractometer was used to determine objective refraction. The best corrected distance visual acuity was assessed with a Monoyer chart. The ocular axial length was assessed using optical biometry. The applanation tonometry was used to determine intraocular pressure. Peripapillary retinal nerve fibre layer thickness (pRNFLT), macular total retinal thickness, and ganglion cell complex (GCC) were measured using optical coherence tomography. Ocular axial length is reduced after DI. pRNFL is thickest after DI specifically in the temporal, temporal-inferior, and nasal-inferior quadrants. Macular total retinal at the inferior quadrant of the 6-mm ring is thickest after DI. Global GCC is thinnest after DI. In this study, 5 days of DI induces slight but significant ophthalmological changes in women. However, these subtle changes do not correspond to criteria defined in SANS.
Synthesis of UV-resistant and colorless polyimide films for optoelectrical applications
Authors: Xi Le , Lv Yadong , Feng Jiabao , Huang Yanyan , Huang Yajiang , Yang Qi , Li Guangxian , Kong Miqiu 
Published: January 2024

AbstractDue to their excellent mechanical properties and intrinsic flexibility, polyimides (PIs) are promising candidates for optoelectrical applications under harsh conditions such as flexible organic solar cells as well as flexible smart windows, etc. Much progress has been made on their optical transmittance; however, there remain significant concerns about their environmental stability, particularly their UV resistance. Herein, 4 types of colorless polyimides (CPIs) with different molecular structures containing trifluoromethyl, ethers, or fluorenes are carefully designed, and the dependence of their UV resistance on structures is explored systematically. It is found that the introduction of isopropylidene, ethers and fluorenes effectively enhances the UV resistance of CPI and its initial performance (optical transparency, thermal stability, and toughness) simultaneously as a result of the subtle manipulation of the conjugation structures. Specifically, the optimized polyimide film shows decent optical properties ($${T}_{550{\rm{nm}}}$$

T

550
nm

~ 88%, yellowness index ~3.26), and thermal stability ($${T}_{5 \% }$$

T

5
%

~ 503 °C in N2 atmosphere, $${T}_{{\rm{g}}}$$

T

g

~ 312 °C). Moreover, after high-intensity UV irradiation, CPI not only maintains over 90% of mechanical properties but also retains excellent optical properties ($${T}_{550{\rm{nm}}}$$

T

550
nm

~ 88%) and thermal stability ($${T}_{5 \% }$$

T

5
%

~ 506 °C). The design strategy paves the way for enhancing the durability of PIs for energy conversion and electronic applications resisting harsh conditions.
Multi-site benchmark classification of major depressive disorder using machine learning on cortical and subcortical measures
Authors: Belov Vladimir , Erwin-Grabner Tracy , Aghajani Moji , Aleman Andre , Amod Alyssa R. , Basgoze Zeynep , Benedetti Francesco , Besteher Bianca , Bülow Robin , Ching Christopher R. K. , Connolly Colm G. , Cullen Kathryn , Davey Christopher G. , Dima Danai , Dols Annemiek , Evans Jennifer W. , Fu Cynthia H. Y. , Gonul Ali Saffet , Gotlib Ian H. , Grabe Hans J. , Groenewold Nynke , Hamilton J Paul , Harrison Ben J. , Ho Tiffany C. , Mwangi Benson , Jaworska Natalia , Jahanshad Neda , Klimes-Dougan Bonnie , Koopowitz Sheri-Michelle , Lancaster Thomas , Li Meng , Linden David E. J. , MacMaster Frank P. , Mehler David M. A. , Melloni Elisa , Mueller Bryon A. , Ojha Amar , Oudega Mardien L. , Penninx Brenda W. J. H. , Poletti Sara , Pomarol-Clotet Edith , Portella Maria J. , Pozzi Elena , Reneman Liesbeth , Sacchet Matthew D. , Sämann Philipp G. , Schrantee Anouk , Sim Kang , Soares Jair C. , Stein Dan J. , Thomopoulos Sophia I. , Uyar-Demir Aslihan , van der Wee Nic J. A. , van der Werff Steven J. A. , Völzke Henry , Whittle Sarah , Wittfeld Katharina , Wright Margaret J. , Wu Mon-Ju , Yang Tony T. , Zarate Carlos , Veltman Dick J. , Schmaal Lianne , Thompson Paul M. , Goya-Maldonado Roberto ,   the ENIGMA Major Depressive Disorder working group
Published: January 2024

AbstractMachine learning (ML) techniques have gained popularity in the neuroimaging field due to their potential for classifying neuropsychiatric disorders. However, the diagnostic predictive power of the existing algorithms has been limited by small sample sizes, lack of representativeness, data leakage, and/or overfitting. Here, we overcome these limitations with the largest multi-site sample size to date (N = 5365) to provide a generalizable ML classification benchmark of major depressive disorder (MDD) using shallow linear and non-linear models. Leveraging brain measures from standardized ENIGMA analysis pipelines in FreeSurfer, we were able to classify MDD versus healthy controls (HC) with a balanced accuracy of around 62%. But after harmonizing the data, e.g., using ComBat, the balanced accuracy dropped to approximately 52%. Accuracy results close to random chance levels were also observed in stratified groups according to age of onset, antidepressant use, number of episodes and sex. Future studies incorporating higher dimensional brain imaging/phenotype features, and/or using more advanced machine and deep learning methods may yield more encouraging prospects.
Changes in microbial community structure and yield responses with the use of nano-fertilizers of nitrogen and zinc in wheat–maize system
Authors: Upadhyay Pravin Kumar , Dey Abir , Singh Vinod Kumar , Dwivedi Brahma Swaroop , Singh Rajiv Kumar , Rajanna G. A. , Babu Subhash , Rathore Sanjay Singh , Shekhawat Kapila , Rai Pradeep Kumar , Choudhury Nalini Kanta , Budhlakoti Neeraj , Mishra Dwijesh Chandra , Rai Anil , Singh Awtar , Bhardwaj Ajay Kumar , Shukla Gaurav 
Published: January 2024

AbstractThe growing popularity of nano-fertilization around the world for enhancing yield and nutrient use efficiency has been realized, however its influence on soil microbial structure is not fully understood. The purpose of carrying out this study was to assess the combined effect of nano and conventional fertilizers on the soil biological indicators and crop yield in a wheat–maize system. The results indicate that the at par grain yield of wheat and maize was obtained with application of 75% of recommended nitrogen (N) with full dose of phosphorus (P) and potassium (K) through conventional fertilizers along with nano-N (nano-urea) or nano-N plus nano-Zn sprays and N100PK i.e. business as usual (recommended dose of fertilizer). Important soil microbial property like microbial biomass carbon was found statistically similar with nano fertilizer-based management (N75PK + nano-N, and N75PK + nano-N + nano-Zn) and conventional management (N100PK), during both wheat and maize seasons. The experimental data indicated that the application of foliar spray of nano-fertilizers along with 75% N as basal is a sustainable nutrient management approach with respect to growth, yield and rhizosphere biological activity. Furthermore, two foliar sprays of nano-N or nano-N + nano-Zn curtailed N requirement by 25%, furthermore enhanced soil microbial diversity and the microbial community structure. The specific microbial groups, including Actinobacteria, Bacteroidia, and Proteobacteria, were present in abundance and were positively correlated with wheat and maize yield and soil microbial biomass carbon. Thus, one of the best nutrient management approaches for sustaining productivity and maintaining sound microbial diversity in wheat–maize rotation is the combined use of nano-fertilizers and conventional fertilizers.