bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enabling Long‐term Cycling Stability of Na3V2(PO4)3/C vs. Hard Carbon Full‐cells
Authors: Stüble Pirmin , Müller Cedric , Klemens Julian , Scharfer Philip , Schabel Wilhelm , Häringer Marcel , Binder Joachim R. , Hofmann Andreas , Smith Anna 
Published: December 2023

AbstractSodium‐ion batteries are becoming an increasingly important complement to lithium‐ion batteries. However, while extensive knowledge on the preparation of Li‐ion batteries with excellent cycling behavior exists, studies on applicable long‐lasting sodium‐ion batteries are still limited. Therefore, this study focuses on the cycling stability of batteries composed of Na3V2(PO4)3/C based cathodes and hard carbon anodes. It is shown that full‐cells with a decent stability are obtained for ethylene carbonate/propylene carbonate electrolyte and the conducting salt NaPF6. With cathode loadings of 1.2 mAh/cm2, after cell formation discharge capacities up to 92.6 mAh/g are obtained, and capacity retentions >90 % over 1000 charge/discharge cycles at 0.5 C/0.5 C are observed. It is shown that both, the additive fluoroethylene carbonate and impurities in the electrolyte, negatively affect the overall discharge capacity and cycling stability and should therefore be avoided. Remarkably, the internal resistances of well‐balanced and well‐built cells did not increase over 1500 cycles and 5 months of testing, which is a very promising result regarding the possible lifespan of the cells. The initial loss of active sodium ions in hard carbon remains a major problem, which can only be partially reduced by proper balancing.
Conceptualising quality early childhood education: Learning from young children in Brazil and South Africa through creative and play‐based methods
Authors: Wright Laura H. V. , Rizzini Irene , Gwele Malibongwe , McNair Lynn , Porto Cristina Laclette , Orgill Marsha , Tisdall E. Kay M. , Bush Malcolm , Biersteker Linda 
Published: December 2023

AbstractEarly childhood has increasingly been acknowledged as a vital time for all children. Inclusive and quality education is part of the United Nations Sustainable Development Goals, with the further specification that all children have access to quality pre‐primary education. As early childhood education (ECE) has expanded worldwide, so have concerns about the quality of ECE provision, including whether its pedagogy is culturally meaningful and contextually appropriate. While these issues are much debated in themselves, often missing is a key stakeholder group for such discussions: young children. Young children have critical insights and perspectives of key importance for ensuring quality ECE. This article addresses how quality ECE can be conceptualised, through reflections on creative and play‐based methods with young children, used in a cross‐national project titled Safe Inclusive Participative Pedagogy. The article draws on community case studies undertake by two of the country teams in Brazil and South Africa. In contexts where children's participation is not necessarily familiar in ECE settings nor understood by key stakeholders, the fieldwork shows that children can express their views and experiences through using creative and play‐based methods. We argue that these methods can become part of a critical pedagogy through ECE settings, where ECE practitioners, children and other key stakeholders engage in ongoing, challenging and transformative dialogue. In turn, critical pedagogy has the potential to strengthen local practices, challenge top‐down approach, and foster quality safe, inclusive, participative early years education.
Endogenous chemicals guard health through inhibiting ferroptotic cell death
Authors: Song Yuan‐Hao , Lei Hong‐Xu , Yu Dou , Zhu Hao , Hao Meng‐Zhu , Cui Rong‐Hua , Meng Xiang‐Shuai , Sheng Xie‐Huang , Zhang Lei 
Published: December 2023

AbstractFerroptosis is a new form of regulated cell death caused by iron‐dependent accumulation of lethal polyunsaturated phospholipids peroxidation. It has received considerable attention owing to its putative involvement in a wide range of pathophysiological processes such as organ injury, cardiac ischemia/reperfusion, degenerative disease and its prevalence in plants, invertebrates, yeasts, bacteria, and archaea. To counter ferroptosis, living organisms have evolved a myriad of intrinsic efficient defense systems, such as cyst(e)ine‐glutathione‐glutathione peroxidase 4 system (cyst(e)ine‐GPX4 system), guanosine triphosphate cyclohydrolase 1/tetrahydrobiopterin (BH4) system (GCH1/BH4 system), ferroptosis suppressor protein 1/coenzyme Q10 system (FSP1/CoQ10 system), and so forth. Among these, GPX4 serves as the only enzymatic protection system through the reduction of lipid hydroperoxides, while other defense systems ultimately rely on small compounds to scavenge lipid radicals and prevent ferroptotic cell death. In this article, we systematically summarize the chemical biology of lipid radical trapping process by endogenous chemicals, such as coenzyme Q10 (CoQ10), BH4, hydropersulfides, vitamin K, vitamin E, 7‐dehydrocholesterol, with the aim of guiding the discovery of novel ferroptosis inhibitors.
Development of a multifunctional bioreactor to evaluate the promotion effects of cyclic stretching and electrical stimulation on muscle differentiation
Authors: Hu Wei‐Wen , Chen Yen‐Chi , Tsao Chia‐Wen , Chen Shen‐Liang , Tzeng Chung‐Yuh 
Published: December 2023

AbstractA multifunctional bioreactor was fabricated in this study to investigate the facilitation efficiency of electrical and mechanical stimulations on myogenic differentiation. This bioreactor consisted of a highly stretchable conductive membrane prepared by depositing polypyrrole (PPy) on a flexible polydimethylsiloxane (PDMS) film. The tensile deformation of the PPy/PDMS membrane can be tuned by adjusting the channel depth. In addition, PPy/PDMS maintained its electrical conductivity under continuous cyclic stretching in the strain range of 6.5%–13% for 24 h. This device can be used to individually or simultaneously perform cyclic stretching and electrical stimulation. The results of single stimulation showed that either cyclic stretching or electrical stimulation upregulated myogenic gene expression and promoted myotube formation, where electrical stimulation improved better than cyclic stretching. However, only cyclic stretching can align C2C12 cells perpendicular to the stretching direction, and electrical stimulation did not affect cell morphology. Myosin heavy chain (MHC) immunostaining demonstrated that oriented cells under cyclic stretching resulted in parallel myotubes. The combination of these two stimuli exhibited synergetic effects on both myogenic gene regulation and myotube formation, and the incorporated electrical field did not affect the orientation effect of the cyclic stretching. These results suggested that these two treatments likely influenced cells through different pathways. Overall, the simultaneous application of cyclic stretching and electrical stimulation preserved both stimuli's advantages, so myo‐differentiation can be highly improved to obtain abundant parallel myotubes, suggesting that our developed multifunctional bioreactor should benefit muscle tissue engineering applications.
Systematic review of nerves at risk at the wrist in common surgical approaches to the forearm: Anatomical variations and surgical implications
Authors: Bridgwater Hannah , Mertz Tamara , Brassett Cecilia , Ashwood Neil 
Published: December 2023

AbstractThree commonly used approaches to the forearm in orthopedic surgery are Henry's, Thompson's, and the ulnar approach, each of which has the potential to cause injury to nerves around the wrist. Preserving these nerves is important to prevent complications such as neuroma formation and motor and sensory changes to the hand. We conducted a review of the literature to assess the nerves at risk and whether ‘safe zones’ exist to avoid these nerves. An independent reviewer conducted searches in Embase and MEDLINE of the literature from 2010 to 2020. A total of 68 papers were identified, with 18 articles being included in the review. Multiple nerves were identified as being at risk for each of the approaches described. In the anterior approach, the palmar cutaneous branch of the median nerve (PCBMN) is most at risk of injury. An incision immediately radial to the flexor carpi radialis (FCR) or directly over the FCR is most likely to avoid injury to both superficial branch of the radial nerve (SBRN) and PCBMN. With Thompson's approach, the safest zone for an incision is directly over or slightly radial to Lister's tubercle to avoid injury to SBRN and lateral cutaneous nerve of the forearm. For the ulnar approach, a safe zone was shown to be on the ulnar side of the wrist around the ulnar styloid (US) when the forearm was in supination or a neutral position to avoid injury to the dorsal branch of the ulna nerve (DBUN). Care must be taken around the US due to the density of nerves and the proximity of the last motor branch of the posterior interosseous nerve to the ulnar head. This review highlighted the proximity of nerves to the three most common surgical incisions used to access the forearm. In addition, anatomical variations may exist, and each of the nerves identified as being at risk has multiple branches. Both factors increase the potential of intraoperative damage if the anatomy is not properly understood. The surgeon must adhere carefully to the established approaches to the wrist and distal forearm to minimize damage to nerves and optimize surgical outcomes for the patient.
Adjuvant chemotherapy combined with immunotherapy in patients with cholangiocarcinoma after radical resection
Authors: Li Xiao‐hui , Zhou En‐liang , Zhao Chong‐yu , Cui Bo‐kang , Dong Xiao‐yuan , Du Hang , Lin Xiao‐jun 
Published: December 2023

AbstractBackgroundThe malignancy of cholangiocarcinoma is highly pronounced, and it exhibits a propensity for recurrence and metastasis even in the presence of standard chemotherapy. The efficacy of adjuvant chemotherapy combined with immunotherapy in patients with resected cholangiocarcinoma needs to be substantiated.MethodsData from 101 patients with cholangiocarcinoma treated at the Sun Yat‐sen University Cancer Center between 2015 and 2020 were studied.ResultsAfter propensity score matching, there were no significant differences in baseline characteristics between patients in the combined adjuvant chemotherapy and immunotherapy group (AC + IM group) and the adjuvant chemotherapy alone group (AC group) (all p > 0.05). The AC + IM group demonstrated a statistically significant improvement in relapse‐free survival (RFS) compared to the AC group (p = 0.032). Likewise, the AC + IM group exhibited a significantly superior overall survival (OS) outcome when compared to the AC group (p = 0.044). Multivariate Cox analysis unveiled perineural invasion (p = 0.041), lymph node metastasis (p = 0.006), and postoperative immunotherapy (p = 0.008) as independent prognostic factors exerting a significant impact on the OS of patients. In the cohort of patients with perineural invasion, the AC + IM group exhibited significantly improved OS compared to the AC group (p = 0.0077). Similarly, within the subset of patients with lymph node metastasis, the AC + IM group exhibited a significantly superior OS outcome when compared to the AC group (p = 0.023).ConclusionCombining postoperative adjuvant chemotherapy with immunotherapy extends the RFS and OS of patients with cholangiocarcinoma following radical resection.
Addition of immune checkpoint inhibitors to chemotherapy versus chemotherapy alone in patients with triple‐negative breast cancer: A systematic review and meta‐analysis
Authors: Gao Xin , Zhu Ying , Wang Peipei , Yu Lulin , Ruan Shanming , Shen Minhe , Zhang Kai 
Published: December 2023

AbstractBackgroundTriple‐negative breast cancer (TNBC) is a relatively common malignant tumor with high mortality rates. There are limited treatment options and current therapy regimens often fall short of providing positive outcomes. The development of immune checkpoint inhibitors (ICIs) have provided a vital treatment option although efficacy has varied. Here, we review patient response to current TNBC treatment with and without the addition of ICIs.MethodsA systematic search of PubMed, Cochrane, and EMBASE library databases was done to search eligible studies published from their inception through April 3, 2022. The primary outcome indicators used were progression‐free survival (PFS), overall survival (OS), pathological complete response rate (pCR) and objective remission rate (ORR), while adverse events (AEs) were also analyzed. Publication bias and sensitivity analyses and were performed to evaluate the quality of assessment.ResultsOverall, the meta‐analysis looked at seven randomized controlled trials (RCTs) that included 4631 patients with TNBC. Results showed an improvement in PFS for patients receiving ICI in addition to chemotherapy (CT) in both the intent‐to‐treat (ITT) population and PD‐L1 positive patients. Increased pCR rates were observed in all patients irrespective of PD‐L1 status as well as increased ORR in the ITT which was more notable in PD‐L1 positive subjects. While significant improvement in OS was observed only in PD‐L1 positive individuals, the use of ICIs plus CT resulted in severe adverse reactions, specifically immune‐related.ConclusionsThis study supports the increased efficacy of ICIs in combination with CT compared to CT alone in patients with TNBC, with the most notable benefit observed in PD‐L1 positive patients. However, combination therapy increases the risk of adverse reactions which warrants further investigation.
The heterogeneous impact of targeted therapy on the prognosis of stage III/IV colorectal cancer patients with different subtypes of TP53 mutations
Authors: Chen Jie , Chang Xiaona , Li Xinyi , Liu Jiaying , Wang Na , Wu Ying , Zheng Liduan , Nie Xiu 
Published: December 2023

AbstractBackgroundThe relationship between molecular characteristics and the prognosis of colorectal cancer (CRC) patients has not been fully understood. This study explored the impact of targeted therapy on the prognosis of CRC patients with different TP53 mutations, in the context of comprehensive treatment.MethodsThis study included patients with stage III/IV primary CRC from the electronic medical record system. TP53 mutations were detected via next‐generation sequencing (NGS) using formalin‐fixed paraffin‐embedded (FFPE) tissues. Applying two methods, we classified TP53 mutations as gain of function (GOF)/non‐GOF mutations or known/likely loss of function (LOF) mutations. Kaplan–Meier plot and parametric survival analysis were performed to evaluate the prognosis of CRC patients and identify potential predictors.ResultsThere were 286 patients included, of which 166 (58.04%) patients received targeted therapy and 120 (41.96%) did not. There were 286 patients in the TP53 GOF classification set and 247 in the TP53 LOF classification set. Parametric survival analysis, adjusted for sex, onset, KRAS mutation, sidedness, stage, and surgery, showed that receiving targeted therapy predicted better overall survival (OS) among patients who harbored TP53 GOF mutations (HR 0.40, 95% confidence interval (CI) [0.21, 0.76], p = 0.005) or known LOF mutations (HR 0.21, 95% CI [0.07, 0.60], p = 0.002). However, there was no significant impact of receiving targeted therapy on OS among patients harboring TP53 non‐GOF mutations (HR 1.68, 95% CI [0.50, 5.63], p = 0.403) or likely LOF mutations (HR 0.90, 95% CI [0.34, 2.39], p = 0.837).ConclusionsReceiving targeted therapy had a heterogeneous impact on the prognosis of CRC patients harboring different TP53 mutations. These results provide promising value for future personalized treatment and precision medicine.
The effectiveness of blinatumomab in clearing measurable residual disease in pediatric B‐cell acute lymphoblastic leukemia patients detected by next‐generation sequencing
Authors: Gu Min'er , Xia Yahong , Zhang Jingying , Tang Yongmin , Xu Weiqun , Song Hua , Xu Xiaojun 
Published: December 2023

AbstractBackgroundBlinatumomab improved survival outcomes in B‐cell acute lymphoblastic leukemia (B‐ALL) patients with measurable residual disease (MRD) <10−4. However, data on blinatumomab clearing MRD with high sensitivity of 10−6 remain scarce. This study evaluates the effectiveness of blinatumomab in eradicating extremely low level (up to <10−6) of MRD, as detected by next‐generation sequencing (NGS), in children with B‐ALL.MethodsPatients (n = 19) whose MRD was undetectable by multiparameter flow cytometry (MFC) (sensitivity of 10−4) but detectable by NGS after chemotherapy and followed by blinatumomab consolidation were included retrospectively.ResultsAfter one course of blinatumomab, 13/19 patients (68%) successfully achieved NGS‐MRD clearance (undetectable). With a median follow‐up of 13.3 months, three of patients who were NGS‐MRD positive relapsed within 1.8 months, while another three remained complete remission.ConclusionsOur study was the first to demonstrate that blinatumomab could further eradicate MRD after patients achieve MFC‐MRD undetectable in B‐ALL patients.
Efficacy and outcome of molecular targeted therapies in elderly patients with hepatocellular carcinoma: Relative dose intensity associated with overall survival
Authors: Oura Kyoko , Morishita Asahiro , Takuma Kei , Nakahara Mai , Tadokoro Tomoko , Fujita Koji , Mimura Shima , Tani Joji , Ono Masafumi , Himoto Takashi , Masaki Tsutomu 
Published: December 2023

AbstractAimIndications of drug therapies to elderly patients with hepatocellular carcinoma (HCC) should be carefully determined. The current study assessed the safety and efficacy of molecular targeted agents (MTAs) in the elderly patients with HCC, and identified factors associated with prognosis in a real‐world clinical setting.MethodsIn a retrospective observational study, clinical data of patients with unresectable HCC treated with sorafenib or lenvatinib as first‐line treatment at our hospital between 2011 and 2022, were investigated. Clinical parameters, therapeutic effects, adverse events (AEs), and prognosis were evaluated separately for the non‐elderly (<75 years old) and elderly patients (≥75 years old).ResultsOverall, 111 patients were enrolled, including 59 non‐elderly and 52 elderly patients. Compared to the non‐elderly patients, the elderly patients had significantly lower skeletal muscle mass and a significantly lower percentage of patients in poor general condition with performance status 2 or higher, but there were no differences in parameters related to liver function or nutritional status. There were no significant differences in the incidence of severe AEs and therapeutic effects between the groups. No significant difference in progression‐free survival was observed in the elderly and non‐elderly patients; however, overall survival (OS) for sorafenib treatment was shorter in the elderly patients than in the non‐elderly patients. Elderly patients consumed lower doses of both the drugs, and relative dose intensity (RDI) 4 weeks after treatment (4W‐RDI) was associated with OS. Further, OS in the elderly patients was significantly longer in the subgroup with high 4W‐RDI as compared to that in the subgroup with low 4W‐RDI.ConclusionsMTAs can be safely administered to elderly patients with HCC. Furthermore, 4W‐RDI is associated with longer OS. Maintaining RDI in the early phase is crucial in predicting the success of treatment with MTAs, especially in the elderly patients.
The efficacy of Linum usitatissimum seeds to inhibit estrogen receptor as a natural therapy for PCOS: An in silico and in vitro analysis
Authors: Ahmad Fatima , Ahmed Abrar , Shakeel Alishba , Hussain Hafiza A. , Raza Syed A. 
Published: December 2023

AbstractPolycystic ovarian syndrome (PCOS) is an endocrinological disorder aroused due to hormonal disturbances. It is characterized by anovulation due to an excess of androgen and estrogen hormones, thus leading to the formation of multiple cysts, imposing life‐threatening conditions. This manuscript aimed to introduce a natural estrogen receptor (ESR) inhibitors that can provide protection against PCOS. The computational analysis of Linum usitatissimum seeds compounds against ESR alpha receptor was performed, and the binding affinities of the ligand compounds and receptor proteins were scrutinized. Nine lignin compounds were docked, and the results were compared with that of reference estrogen receptor inhibitors, clomiphene, and tamoxifen. The binding affinity scores for pinoresinol, lariciresinol, secoisolariciresinol, and matairesinol were −10.67, −10.66, −10.91, and −10.60 kcal mol−1, respectively. These were comparable to the binding affinity score of reference compounds −11.406 kcal mol−1 for clomiphene and −10.666 kcal mol−1 for tamoxifen. Prime MM‐GBSA studies showcased that Linum usitatissimum seeds compounds exhibit significant efficacy and efficiency towards receptor protein. Moreover, MD‐simulation studies were performed and the results depict that the lignin compounds form stable complexes at 300 K throughout the simulation time. For further clarity, in‐vitro experiments were carried out. The results exhibit the decline in cell proliferation in a concentration‐dependent manner by extract 1 (ethyl acetate) EX1 and extract 2 (petroleum ether) EX2. Hence, providing evidence regarding the anti‐estrogenic activity of the sample extracts. Collectively, these results showed that flax seed can reduce the levels of estrogen, which can induce ovulation and prevent cyst formation, and ultimately can provide protection against PCOS.
Engineering of Candida antarctica Lipase B for Enhanced Perhydrolysis Activity by Modulating the Potential Water Channel
Authors: Jeon Minjeong , Park Seongsoon 
Published: December 2023

AbstractAlthough Candida antarctica lipase B (CAL‐B) naturally hydrolyzes an ester compounds, it can catalyze a variety of promiscuous reactions, such as perhydrolysis, aldol reaction, and Michael‐type reactions. Perhydrolysis is similar to hydrolysis but uses hydrogen peroxide instead of water molecules. It was recently proposed that water molecules access the active site of CAL‐B through a water channel. We hypothesized that hydrogen peroxide may also use the channel to enter the active site, and that the access of hydrogen peroxide is one of the key factors for perhydrolysis activity. We investigated the effects of mutations at two residues in the water channel of CAL‐B on the perhydrolysis activity. The residues, Pro280 and Ala281, are located at the constricted area of the water channel. We found that the substitution of Ala281 to a small and polar amino acid, such as serine or threonine, resulted in a noticeable increase in hydrolysis and perhydrolysis activity. The activity of the variant enzymes was up to three times and twice higher for the activities of hydrolysis and perhydrolysis, respectively, than those of CAL‐B. In contrast, the substitution of the Pro280 residue with a small and polar amino acid decreased both hydrolysis and perhydrolysis activities.
Survey of herbicide and fungicide use in peanut in North Carolina and Virginia in the United States
Authors: Jordan David L. , Anco Dan , Balota Maria , Langston David , Lux LeAnn , Shew Barbara , Brandenburg Rick L. 
Published: December 2023

Plain Language SummaryDiseases and weeds can reduce yield of peanut (Arachis hypogaea L.) if effective control measures including fungicides and herbicides are not used. A survey of 76 farmers in North Carolina and Virginia at Cooperative Extension Service meetings was completed relative to fungicide and herbicide use in 2021. Eighty‐two percent of farmers made between three and five herbicide applications, while 68% of farmers made between four and five fungicide applications. 2,4‐DB was the herbicide applied most frequently. The fungicide chlorothalonil was applied the most often. Pydiflumetofen was applied by 61% of farmers. Results from this survey can be used to inform regulatory agencies on pesticide use patterns by defining the relative importance of individual or groups of pesticides currently used to suppress pests and prevent or mitigate associated economic losses.