bluuebunny/crossref_metadata_2025_split huggingface dataset:
EF-Hand Calcium Sensor, EfhP, Controls Transcriptional Regulation of Iron Uptake by Calcium inPseudomonas aeruginosa
Authors: Burch-Konda Jacob , Kayastha Biraj B. , Kubo Aya , Achour Myriam , Hull Mackenzie , Braga Reygan , Winton Lorelei , Rogers Rendi R. , McCoy Jacee , Lutter Erika I. , Patrauchan Marianna A. 
Published: January 2024

ABSTRACTThe human pathogenPseudomonas aeruginosaposes a major risk for a range of severe infections, particularly lung infections in patients suffering from cystic fibrosis (CF). As previously reported, the virulent behavior of this pathogen is enhanced by elevated levels of Ca2+that are commonly present in CF nasal and lung fluids. In addition, a Ca2+-binding EF-hand protein, EfhP (PA4107), was partially characterized and shown to be critical for the Ca2+-regulated virulence inP. aeruginosa. Here we describe the rapid (10 min, 60 min), and adaptive (12 h) transcriptional responses of PAO1 to elevated Ca2+detected by genome-wide RNA sequencing and show thatefhPdeletion significantly hindered both rapid and adaptive Ca2+regulation. The most differentially regulated genes included multiple Fe sequestering mechanisms, a large number of extracytoplasmic function sigma factors (ECFσ) and several virulence factors, such as production of pyocins. The Ca2+regulation of Fe uptake was also observed in CF clinical isolates and appeared to involve the global regulator Fur. In addition, we showed that theefhPtranscription is controlled by Ca2+and Fe, and this regulation required Ca2+-dependent two-component regulatory system CarSR. Furthermore, theefhPexpression is significantly increased in CF clinical isolates and upon pathogen internalization into epithelial cells. Overall, the results established for the first time that Ca2+controls Fe sequestering mechanisms inP. aeruginosaand that EfhP plays a key role in the regulatory interconnectedness between Ca2+and Fe signaling pathways, the two distinct and important signaling pathways that guide the pathogen’s adaptation to host.IMPORTANCEPseudomonas aeruginosa(Pa) poses a major risk for severe infections, particularly in patients suffering from cystic fibrosis (CF). For the first time, kinetic RNA sequencing analysis identifiedParapid and adaptive transcriptional responses to Ca2+levels consistent with those present in CF respiratory fluids. The most highly upregulated processes include iron sequestering, iron starvation sigma factors, and self-lysis factors pyocins. An EF-hand Ca2+sensor, EfhP, is required for at least 1/3 of the Ca2+response, including all the iron uptake mechanisms and production of pyocins. Transcription ofefhPitself is regulated by Ca2+, Fe, and increases during interactions with host epithelial cells, suggesting the protein’s important role inPainfections. The findings establish the regulatory interconnectedness between Ca2+and iron signaling pathways that shapePatranscriptional responses. Therefore, understanding Pa’s transcriptional response to Ca2+and associated regulatory mechanisms will serve the development of future therapeutics targetingPadangerous infections.
Microbial communities associated with marine sponges from diverse geographic locations harbour biosynthetic novelty
Authors: Nowak Vincent V , Hou Peng , Owen Jeremy G 
Published: January 2024

AbstractMarine sponges are a prolific source of biologically active small molecules, many of which originate from sponge-associated microbes. Identifying the producing microbes is a key challenge in developing sustainable routes for production and isolation of sponge-associated metabolites, and requires application of several computational tools. To facilitate these analyses, we developed MetaSing, a reproducible singularity-based pipeline for assembly, identification of high quality metagenome-assembled genomes (MAGs), and analysis biosynthetic gene clusters (BGCs) from metagenomic short read data. We apply this pipeline to metagenome datasets from 16 marine sponges collected from New Zealand, Tonga and the Mediterranean Sea. Our analysis yielded 643 MAGs representing 510 species. Of the 2,670 BGCs identified across all samples, 70.8% were linked to a MAG, enabling taxonomic characterisation. Further comparison of BGCs to those identified from previously sequenced microbes revealed high biosynthetic novelty in variety of underexplored phyla including Poribacteria, Acidobacteriota and Dadabacteria. Alongside the observation that each sample contains unique biosynthetic potential, this holds great promise for natural product discovery and for furthering the understanding of different sponge holobionts.
A Genetic Screen inDrosophilauncovers a role forsenseless-2in surface glia in the peripheral nervous system to regulate CNS morphology
Authors: Lacin Haluk , Zhu Yuqing , DiPaola Jose T. , Wilson Beth A. , Zhu Yi , Skeath James B. 
Published: January 2024

AbstractDespite increasing in mass approximately 100-fold during larval life, theDrosophilaCNS maintains its characteristic form during this rapid growth phase. Dynamic interactions between the overlying basement membrane and underlying surface glia are known to regulate CNS structure inDrosophila, but the genes and pathways that establish and maintain CNS morphology during development remain poorly characterized. To identify genes that regulate CNS shape inDrosophila, we conducted an EMS-based, forward genetic screen of the second chromosome, uncovered 50 mutations that disrupt CNS structure, and mapped these alleles to 17 genes. Whole genome sequencing revealed the affected gene for all but one mutation. Identified genes include well characterized regulators of tissue shape, likeLanB1, viking, and Collagen type IV alpha1, as well as previously characterized genes, such asToll-2andRme-8, with no known role in regulating CNS structure. We also uncovered thatpapilinandC1GalTAlikely act in the same pathway to regulate CNS structure and found that the fly homolog of a glucuronosyltransferase, B4GAT1/LARGE1, that regulates Dystroglycan function in mammals is required to maintain CNS shape inDrosophila. Finally, we show that thesenseless-2transcription factor is expressed and functions specifically in surface glia found on peripheral nerves but not those on the CNS proper to govern CNS structure, identifying a gene that functionally subdivides a glial subtype along the peripheral-central axis. Future work on these genes should clarify the genetic mechanisms that ensure the homeostasis of CNS shape and form during development.
Distinct effects of phyllosphere and rhizosphere microbes on invaderAgeratina adenophoraduring its early life stages
Authors: Zeng Zhao-Ying , Huang Jun-Rong , Liu Zi-Qing , Yang Ai-Ling , Li Yu-Xuan , Wang Yong-Lan , Zhang Han-Bo 
Published: January 2024

AbstractMicrobes strongly affect invasive plant growth. However, how the phyllosphere and rhizosphere soil microbes distinctively affect seedling mortality and the growth of invasive plants across ontogeny under varying soil nutrient levels remains unclear. In this study, we used the invaderAgeratina adenophorato evaluate these effects in plant growth chambers. We found that leaf litter harboured more potential pathogens and thus had more adverse effects on seed germination and seedling survival than soil inoculation. Microbial inoculation at different growth stages altered the microbial community and microbial functions of seedlings, and earlier inoculation had a more adverse effect on seedling survival and growth. In most cases, the soil nutrient level did not affect microbe-mediated seedling growth and the relative abundance of the microbial community and functions involved in seedling growth. The effects of some microbial genera on seedling survival are distinct from those on growth. Moreover, theA. adenophoraseedling-killing effects of fungal strains isolated from dead seedlings by nonsterile leaf inoculation litter exhibited significant phylogenetic signals, by which strains ofAllophomaandAlternariagenerally caused high seedling mortality. Our study stresses the essential role ofA. adenophoralitter microbes in population establishment by regulating seedling density and growth.
Hyperfine structure and isotope shifts of the
(4s2)S01→(4s4p)P11
transition in atomic zinc
Authors: Röser David , Padilla-Castillo J. Eduardo , Ohayon Ben , Thomas Russell , Truppe Stefan , Meijer Gerard , Stellmer Simon , Wright Sid C. 
Published: January 2024

We report absolute frequency, isotope shift, radiative lifetime, and hyperfine structure measurements of the (4s2)S01→(4s4p)P11 (213.8 nm) transition in Zn I using a cryogenic buffer gas beam. Laser-induced fluorescence is collected with two orthogonally oriented detectors to take advantage of differences in the emission pattern of the isotopes. This enables a clear distinction between isotopes whose resonances are otherwise unresolved, and a measurement of the Zn67 hyperfine structure parameters, A(Zn67)=20(2)MHz and B(Zn67)=10(5)MHz. We reference our frequency measurements to an ultralow expansion cavity and achieve an uncertainty at the level of 1 MHz, about 1 percent of the natural linewidth of the transition.

Published by the American Physical Society
2024
Probing factorization violation with vector angularities
Authors: Bijl Pim , Niedenzu Steven , Waalewijn Wouter J. 
Published: January 2024

Factorization underlies all predictions at the Large Hadron Collider, but has only been rigorously proven in a few cases. One of these cases is the Drell-Yan process, pp→Z/γ+X, in the limit of small-boson transverse momentum. We introduce a one-parameter family of observables, that we call vector angularities, of which the transverse momentum is a special case. This enables the study of factorization violation, with a smooth transition to the limit for which factorization has been established. Like the angularity event shapes, vector angularities are a sum of transverse momenta weighted by rapidity, but crucially this is a vector sum rather than a sum of the magnitude of transverse momenta. We study these observables in , using the effect of multiparton interactions (MPI) as a proxy factorization violation, finding a negligible effect in the case where factorization is established but sizable effects away from it. We also present a factorization formula for the cross section, that does not include factorization violating contributions from Glauber gluons, and thus offers a baseline for studying factorization violation experimentally using vector angularities. Our predictions at next-to-leading logarithmic accuracy are in good in agreement with (not including MPI), and can be extended to higher order.

Published by the American Physical Society
2024
Full one-loop radiative corrections to
e+e−→H+H−
in the inert doublet model
Authors: Abouabid Hamza , Arhrib Abdesslam , El Falaki Jaouad , Gong Bin , Xie Wenhai , Yan Qi-Shu 
Published: January 2024

We compute the full one-loop radiative corrections for charged scalar pair production e+e−→H+H− in the inert doublet model. The on-shell renormalization scheme has been used. We take into account both the weak contributions as well as the soft and hard QED corrections. We compute both the real emission and the one-loop virtual corrections using the Feynman diagrammatic method. The resummed cross section is introduced to cure the Coulomb singularity which occurs in the QED corrections. We have analyzed the parameter space of the inert doublet model in three scenarios after taking into account theoretical constraints, the collider experimental bounds, and dark matter search bounds as well. It is found that the weak interaction dominates the radiative corrections, and its size is determined by the triple Higgs coupling λh0H+H−, which is further connected to the mass of the charged scalar. In the scenario where all the constraints are taken into account, we find that for s=250  GeV and s=500  GeV, the weak corrections are around −6%∼−5% and −10%∼−3%, respectively. While for s=1000  GeV, the weak corrections can reach −15%∼+25%. The new feature is that the weak corrections can be positive near the threshold when the charged scalar is heavier than 470 GeV. Six benchmark points for future collider searches have been proposed.

Published by the American Physical Society
2024
Effective field theory for dark matter absorption on single phonons
Authors: Mitridate Andrea , Pardo Kris , Trickle Tanner , Zurek Kathryn M. 
Published: January 2024

Single phonon excitations, with energies in the 1–100 meV range, are a powerful probe of light dark matter (DM). Utilizing effective field theory, we derive a framework to compute DM absorption rates into single phonons starting from general DM-electron, proton, and neutron interactions. We apply the framework to a variety of DM models: Yukawa coupled scalars, axionlike particles with derivative interactions, and vector DM coupling via gauge interactions or Standard Model electric and magnetic dipole moments. We find that GaAs or Al2O3 targets can set powerful constraints on a U(1)B−L model, and targets with electronic spin ordering are similarly sensitive to DM coupling to the electron magnetic dipole moment. Lastly, we make the code, (an extension of the existing code which computes general DM–single phonon scattering rates), publicly available.

Published by the American Physical Society
2024
From passion to profit: unveiling the strengths of proactive mindset, perseverance, excellence and education in the entrepreneurial success of MSMEs
Authors: Biswas Abhijeet 
Published: January 2024

PurposeThe study investigates the linkage between personality traits and firm performance. It examines the role of the pursuit of excellence, perseverance, a proactive mindset and formal education in determining the entrepreneurial success of MSMEs.Design/methodology/approachData were collected from 432 MSME entrepreneurs using a structured questionnaire from India's two major industrial towns to analyze the impact of personality traits on firm performance. Structural equation modeling (SEM) was employed to assess the direct and indirect relationships with the help of mediation analysis.FindingsThe findings assert that personality traits improve firm performance and determine the success of MSMEs. The results reveal that the need for achievement, a proactive mindset and the pursuit of excellence are crucial to firm performance. In addition, formal education mediates between perseverance and the pursuit of excellence personality attributes on the one side and firm performance on the other.Research limitations/implicationsThe research has various theoretical and practical implications for entrepreneurs, financial institutions and policymakers. The results could be productively used to nurture the entrepreneurial ecosystem in India.Originality/valueAlthough research on personality traits as a driver of firm performance is growing, the pursuit of excellence, perseverance and proactive mindset attributes as enablers of firm performance have not garnered much attention. The study presents a precise conceptual model by integrating the aforementioned dimensions in the backdrop of an emerging market.
The impact of inflation on local government fiscal health
Authors: Martell Christine R. 
Published: January 2024

PurposeInflation and federal monetary efforts to control it with interest rate hikes have very real and overwhelmingly negative consequences on US local governments following the onset of COVID-19. This study explores the post-pandemic inflationary environment of US local governments; examines the impacts of inflation and high interest rates on local government revenue, operating costs, capital costs, and debt service; reviews local government inflation management strategies, including the use of intergovernmental revenue; and assesses ongoing threats to local government financial health and financial resilience.Design/methodology/approachThis study uses trend and literature analysis to comment on current issues local governments face.FindingsThe study finds that the growth of property values and resulting stability of property tax revenue has been important to local government revenues; that local governments bear very real burdens as operating and capital costs increase; and that the combination of high inflation and interest rates affects local government debt issuance by negatively affecting credit quality and interest costs, leading to municipal market contraction. Local governments have benefitted tremendously from intergovernmental revenue, but would be ill-advised to rely on it.Practical implicationsVulnerabilities owing from revenue mismatch with the economy; inadequate affordable housing, inequality, and social issues; a changing workforce and tight labor market; climate change; and federal fiscal contraction—all of which are exacerbated by high inflation and interest rates—require local governments to act strategically, boldly and collaboratively to achieve fiscal health and financial resilience, and to realize positive returns of investments in people and capital.Originality/valueThis work is unique in addressing the post-pandemic impact of inflation and interest rates on local governments.
Examining the effect of perceived risk, self-efficacy and individual differences on consumer intention to use contactless mobile payment services
Authors: Kamboj Shampy , Matharu Manita , Shukla Yupal 
Published: January 2024

Purpose
The purpose of this study is to examine the influence of perceived risk (perceived susceptibility and perceived severity), self-efficacy and individual differences (personal innovativeness and mobile payment knowledge) on consumers’ intention to use contactless mobile payment services (MPS). Additionally, it also empirically tested the moderating effect of hygiene consciousness in the proposed conceptual model.

Design/methodology/approach
The present research developed a conceptual model based on the health belief model (HBM) and diffusion of innovation theory (DOI) to empirically assess whether and how perceived risk, self-efficacy and individual differences influence consumer intention to use contactless MPS. The data was collected using the survey method from 251 smartphone users and analyzed with structural equation modeling. The moderating role of hygiene consciousness was studied in the relationship that self-efficacy and personal innovativeness shares with consumers’ intention toward contactless MPS.

Findings
The results of this study disclose that among all precursors of consumers’ intention toward contactless MPS, only perceived severity, self-efficacy, personal innovativeness and mobile payment knowledge positively affect consumers’ intention to use contactless MPS. However, the effect of perceived susceptibility on consumers’ intention toward contactless MPS was found to be non-significant. The results further depict that hygiene consciousness acts as a moderator in driving consumers’ intention toward contactless mobile payment services.

Research limitations/implications
The HBM model and DOI, which incorporate perceived risk, self-efficacy, individual differences and hygiene consciousness, give light on the factors influencing consumer intention to use contactless MPS in a pandemic situation such as COVID-19. The study will provide useful insights to marketers on how to frame their strategies in the aftermath of pandemics, as the contactless mobile payment method may be perceived as a defensive behavior during and after pandemics.

Practical implications
The study will provide useful insights to marketers on how to frame their strategies in the aftermath of pandemics, as the contactless mobile payment method may be perceived as a defensive behavior during and after pandemics.

Originality/value
By uniting perceived risk, self-efficacy and individual differences with consumers’ intention toward contactless MPS in a conceptual model, along with examining the moderating role of hygiene consciousness, this work responds to the calls for upcoming research concerning mobile payment systems as means of future payment. Thus, it offers an understanding of particular consumer motivations that may guide consumers’ intention toward contactless MPS through the theoretical lens of HBM and DOI.
Domestic tourism and the effects of behavioral changes, government-media trust and moderation of “maintenance of country image”: theoretical analysis
Authors: Yasir Ammar , Hu Xiaojian , Aktan Murat , Farías Pablo , Rauf Abdul 
Published: January 2024

PurposeContemporary changes have occurred in country-level policies and tourists’ intentions in recent years. The role of maintaining a country’s image is trendy in crisis control but has not yet been discussed in domestic tourism research. Extending the Stimulus Organism Response model, this study aims to focus on “trustable WOM creation” in China. In addition, it aimed to discover how behavioral changes encourage domestic tourism intention (DTI).Design/methodology/approachThis study explored the mediating role of DTI and the moderating role of maintenance of country image (MCI) for trustable word of mouth (WOM) creation. Using the snowball sampling technique, a structural equation modeling analysis (Smart PLS-4) was employed to analyze the data of 487 Chinese tourists.FindingsFindings confirm that behavioral changes positively encourage domestic tourism and discourage international tourism, with significant negative moderation by MCI. MCI has an insignificant positive moderating effect between government-media trust and DTI. Furthermore, DTI positively and directly affects the creation of trustable WOM. In addition, it had a 20% mediation effect (VAF%) between behavioral changes and WOM creation, higher than the rejected mediation effect (12%), in the causal relationship between government-media trust and WOM creation.Practical implicationsWOM creation varies from different behavioral changes, but findings suggest that government-media trust and DTI influenced it significantly. Based on the study findings, the government and media can enhance domestic tourism by maintaining the country’s image. These findings both encourage and control the recovery of tourism.Originality/valueThis study provides a theoretical explanation for tourists' behavioral changes during the pandemic. Moreover, it shows that despite avoiding international tourism due to behavioral changes and government-media trust, MCI moderation with the mediation effect of DTI can create trustable WOM. To the best of the authors’ knowledge, this is the first study to theoretically promote tourism through DTI-induced psychology as a mediator and an organism affect prevailing among Chinese tourists.