bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcription factor 3 enhances hepatocellular carcinoma metastasis by upregulating MMP11
Authors: Tian Hongpeng , Huang Cheng , Hou Huafang , Li Peidong , Zhou Yu , Wen Jing , Zhao Yu , Xiao Zhongxiang , Wu Tianhao , Zhou Tong , Zhang Guangjun , Li Shengjie 
Published: December 2023

Abstract
Transcription factor 3 (TCF3) has a vital role in tumor occurrence and progression. However, the specific functions and underlying mechanisms of dysregulated TCF3 in hepatocellular carcinoma (HCC) is not thoroughly characterized. Thus, we explored the levels and roles of TCF3 in HCC samples. In addition, TCF3 knockdown and overexpression models were developed via lentiviral vectors in HCC cells. Transwell as well as in vivo metastasis experiments were performed to measure the effects of TCF3 on HCC cell metastasis. Then, reverse transcription-quantitative PCR, serial deletion, western blotting, site-directed mutagenesis, chromatin immunoprecipitation and dual-luciferase reporter assays were done to determine the involved pathomechanisms. TCF3 levels were markedly elevated in HCC samples, and correlated with poor prognosis. Besides, overexpressed TCF3 promoted HCC cell invasion as well as migration, while TCF3 knockdown repressed HCC cell growth. In addition, TCF3 mediated MMP11 expressions. MMP11 knockdown repressed TCF3-associated HCC cell migration and invasion while its overexpression attenuated the TCF3 knockdown-mediated repression of HCC growth. In human-derived HCC samples, TCF3 were positively correlated with MMP11 in expression level. Overall, these findings highlighted that TCF3 could be regarded as a prognostic biomarker and HCC metastasis regulator.
Applying LASSO logistic regression for prediction of biliary complications after ex-vivo liver resection and autotransplantation in end-stage hepatic alveolar echinococcosis
Authors: Lin Xin , Shao Yingmei , Zhang Ruiqing , Aji Tuerganaili 
Published: December 2023

Abstract
Background: The purpose of this study was to explore the relevant risk factors for biliary complications (BC) in patients with end-stage hepatic alveolar echinococcosis (HAE) following ex-vivo liver resection and autotransplantation (ELRA) and to establish and visualize a nomogram based on LASSO logistic regression.
Methods: From January 2010 to May 2022, clinical data were collected from patients diagnosed with end-stage hepatic alveolar echinococcosis (HAE) who underwent ELRA treatment at the First Affiliated Hospital of Xinjiang Medical University. LASSO logistic regression analysis was performed using R software version 4.2.2. In the variable selection stage, we applied the lasso regression to select the most valuable variables as candidates for further logistic regression analysis. Afterward, we utilized the variables obtained from the previous stage to construct a binary logistic regression analysis. The aim of this analysis was to elucidate the relationship between perioperative clinical data and postoperative biliary complications. Bootstrapping resampling was used to validate the resulting nomogram, and decision curve analysis (DCA) was performed to evaluate the clinical effectiveness of the model.
Results:The study included a total of 118 patients with end-stage HAE after screening. Among them, 48 patients (40.68%) belonged to the biliary complication group and 70 patients (59.32%) belonged to the non-biliary complication group. Through the above analysis, it was found that age (OR=1.04, 95%CI, 1.0-1.09), biliary dilatation (OR=6.71, 95%CI, 2.62-17.19), and portal hypertension (OR=3.62, 95%CI, 1.22-10.76) were independent risk factors for BC after ELRA, while regular liver resection (OR=0.13, 95%CI, 0.02-0.75) was a protective factor. The area under the receiver operating characteristic curve (AUROC) was 0.8188 (95%CI: 0.7417-0.8958) and the calibration curve for this prediction model were satisfactory. Decision Curve Analysis (DCA) showed that within the 9%-85% range, the nomogram had a greater net benefit than uniform intervention for the entire population.
Conclusion: Age, biliary dilatation and portal hypertension are independent risk factors for biliary complications after ELRA. Postoperative biliary complications can be reduced while preserving liver volume by intraoperative regular liver resection. Appropriate interventions based on the nomogram should be used for patients at high risk of postoperative biliary complications.
Vaccination strategies to identify and reach zero-dose and under- immunized children in crisis-affected states in Sudan: A qualitative study
Authors: Sabahelzain Majdi M. , Almaleeh Alaa , Abdelmagid Nada , Abdalla Omayma , Nor Barni , Mounier-Jack Sandra , Singh Neha S. 
Published: December 2023

Abstract

Background
In 2021, 18.2 million "zero-dose" children did not receive any DTP-containing vaccine. In 99 low-and middle-income countries, 20% of zero-dose children are conflict-affected. There is insufficient evidence on vaccination interventions to identify and reach zero-dose children in these settings. This study aimed to map and assess current vaccination strategies to identify and reach zero-dose and under-vaccinated children the crisis-affected states of South Kordofan, South Darfur and the Blue Nile in Sudan.
Methods
We conducted a cross-sectional qualitative study guided by the IRMA model framework (Identify-Reach-Monitor-Measure-Advocate), developed by Gavi. We conducted 20 individual semi-structured interviews during November and December 2022. We interviewed governmental and non-governmental vaccination stakeholders at federal, state and locality levels. We conducted a thematic analysis of interview transcripts.
Results
Zero-dose and under-immunized children in the study sites are concentrated in opposition-controlled areas, nomadic communities, and remote rural areas. Zero-dose and under-immunized children in accessible areas are identified through routine vaccination strategies and surveillance reports. Various strategies are used in inaccessible areas. This includes tasking local institutions and individuals trusted by communities to identify and reach children, and infrequent integration and co-delivery of routine vaccines with other health interventions such as COVID-19 vaccination and insecticidal net distribution. There is a lack of specific population analysis and guidance from ministries of health to measure and monitor zero-dose and under-immunized children. Advocacy is conflated with mobilization and broadly characterized as ad-hoc activities connected to immunization campaigns only
Conclusions
We conclude that there is a need for further research and testing and adapting innovative and tailored interventions in these settings, particularly in the areas of integrated vaccination delivery, community participation in vaccination, improved data generation and use and advocacy.
Down-regulated miR-10a protects against spinal cord injury by up-regulating SIRT1
Authors: Song Chao , Zhang Yan 
Published: December 2023

Abstract
Background: Spinal cord injury (SCI) is a sensory and motor deficit that greatly reduces patients’ standard of living. MicroRNAs (miRNAs) are essential modulators of gene expression and are associated with various pathological processes, including SCI. This investigation aimed to elucidate miR-10a activity in SCI and its potential interaction with SIRT1, a known inflammation and cell survivalregulator.
Methods:
Lentiviral vectors were used to knock down miR-10a in SCI rats. The locomotion scale called Basso, Beattie, and Bresnahan was utilized to assess SCI rats' hindlimbs' functional recovery. PC12 cells were stimulated by lipopolysaccharide (LPS) to establish an in-vitro SCI model. qRT-PCR was carried out to elucidate miR-10a levels in the spinal cord tissues and PC12 cells. The interaction of miR-10a with SIRT1 was elucidated via luciferase reporter analysis. Immunohistochemistry and western blot assessed SIRT1 protein expression. Cell viability was elucidated via MTT analysis, and an ELISA assay was carried out to investigate inflammatory factors.
Results: This investigation indicated upregulated miR-10a and reduced SIRT1 expression in the SCI rats’ tissues.miR-10a knockdown in SCI rats improved motor function recovery, increased neuronal survival, and reduced levels of inflammatory cytokines. Luciferase reporter assays confirmed that miR-10adirectly targets SIRT1. In PC12 cells, downregulation of miR-10a increased SIRT1 expression, enhanced cell viability, and reduced inflammatory factor levels afterLPS stimulation. Conversely, SIRT1 knockdown inhibited the protective effects of downregulated miR-10a on cell viability and inflammatory responses.
Conclusions: The resultssuggest that miR-10a downregulation protects against SCI by upregulating SIRT1 expression, improving functional recovery, and reducing inflammation. Targeting the miR-10a/SIRT1 axis is a promising strategy for SCItreatment.
Development and validation of a metabolic gene signature for predicting the overall survival of patients in lung adenocarcinoma with lymph node metastasis
Authors: He Jinqi , Tang Haidong , Xia Fan , Liu Yanhua , Zhang Wenjing Zhang , Li Faxiang 
Published: December 2023

Abstract
Purpose
Metabolism reprogramming plays an essential role in cancers. The current study explored the prognostic potential of metabolic genes for lung adenocarcinoma (LUAD) with metastasis.
Methods
The RNA-seq data and clinical data for LUAD patients were retrieved from public databases. LASSO analysis was utilized to construct a multigene signature in the TCGA-LUAD cohort. LUAD patients from the GSE72094, GSE30219, GSE31210 dataset were used for external validation. The effect of altered GNPNAT1 expression on cell migration, invasion and EMT were explored in vitro.
Results
Our results showed that a total of 40 differentially expressed metabolic genes (DEMGs) were identified to be involved in the occurrence and lymph node metastasis of LUAD. Univariate Cox regression analysis demonstrated that 10 DEMGs were correlated with overall survival (OS) of LUAD patients. LASSO regression analysis indicated that the prognostic signature, including ALDOA, MTHFD1L, LDHA, GNPNAT1, POLR3G, GPD1L, PGS1, was developed in the TCGA-LUAD cohort. The prognostic value of this signature was successfully validated in the GSE72094, GSE31210, GSE30219 dataset. Receiver operating characteristic (ROC) curve analysis confirmed this signature's predictive capacity. Functional analysis revealed that several signaling pathways were enriched in the high-risk group. Furthermore, the calibration curve of the prognostic nomogram demonstrated good agreement between the predicted and observed survival rates for each of OS. Further analysis revealed that this signature could be an independent predictor for OS in patients with LUAD. Finally, we successfully detected expression level of 7 hub MGs at the transcription level and firstly found that GNPNAT1 might played an important role on LUAD cells migration, invasion, and EMT.
Conclusion
A novel metabolic gene signature can be used for prognostic prediction in LUAD metastasis. Targeting metabolism may be a therapeutic alternative for LUAD metastasis.
An AMPK-dependent hypoxia-responsive subnucleus of the nucleus tractus solitarius coordinates the hypoxic ventilatory response and protects against apneoa in mice
Authors: MacMillan Sandy , Burns David P. , O'Halloran Ken D. , Evans A. Mark 
Published: December 2023

Abstract
Functional magnetic resonance imaging (fMRI) suggests that the hypoxic ventilatory response is facilitated by the AMP-activated protein kinase (AMPK), not at the carotid bodies, but within a subnucleus (Bregma − 7.5 to -7.1mm) of the nucleus tractus solitarius that exhibits rightsided bilateral asymmetry. Here, we map this subnucleus using cFos expression as a surrogate for neuronal activation and mice in which the genes encoding the AMPK-α1 (Prkaa1) and AMPKα2 (Prkaa2) catalytic subunits were deleted in catecholaminergic cells by Cre expression via the tyrosine hydroxylase promoter. Comparative analysis of brainstem sections, relative to controls, revealed that AMPKα1/α2 deletion inhibited, with rightsided bilateral asymmetry, cFos expression in and thus activation of a neuronal cluster that partially spanned three interconnected anatomical nuclei adjacent to the area postrema: SolDL (Bregma − 7.44mm to -7.48mm), SolDM (Bregma − 7.44mm to --7.48mm) and SubP (Bregma − 7.48mm to -7.56mm). This approximates the volume identified by fMRI. Moreover, these nuclei are known to be in receipt of carotid body afferent inputs, and catecholaminergic neurons of SubP and SolDL innervate aspects of the ventrolateral medulla responsible for respiratory rhythmogenesis. Accordingly, AMPKα1/α2 deletion attenuated hypoxiaevoked increases in minute ventilation, blocked active expiration, decreased sigh frequency and increased apnoea frequency. The metabolic status of these AMPKα1/α2 knockouts and the brainstem and spinal cord catecholamine levels were equivalent to controls. We conclude, that within the brainstem an AMPK-dependent, hypoxia-responsive subnucleus partially spans SubP, SolDM and SolDL, namely SubSolHΙe, and is critical to coordination of active expiration, the hypoxic ventilatory response and defence against apnoea.
The Ornamental white marble use and reuse at the Ottoman palaces of Algiers’ Regency: New knowledge to a provenance study
Authors: Bibimoune Aicha , Chergui Samia , Hamza Mohamed Chérif , Prochaska Walter 
Published: December 2023

Abstract

This multi-disciplinary study examines from a historical and technical approach the supply of ornamental white marble in Ottoman Algiers between the 17th and 19th centuries. Our systematic analysis of Ottoman Regency administration archives, in particular the correspondence between the provincial chiefs of Algiers and Cherchell dating from 1824 to 1825AD, reveals two completely different supply routes for white ornamental marble to Algiers' building sites: the first identifies Genoa as the unique source of imports, while the second confirms the use of the remains from the ancient Roman city of Cherchell/Caesarea. In order to verify these provenances of ornamental white marble, petrographic and geochemical analyses were carried out on fifteen (15) samples of white marble from the decorative elements of the Algiers Citadel: a palatial complex whose construction and subsequent completion correspond to the same period as these exchanges of official correspondence. Experimental results confirm the Italian provenance of some samples, particularly from Carrara, while others appear to be of local origin from Filfila.
Prominent Bioactive Profile of 3d- Transition Metal Doped Schiff’s Base Complexes
Authors: Gajbhiye Rahul 
Published: December 2023

Abstract
A mixed ligand complex were prepared from the Schiff’s base ligand p-Methoxy Isonitroso Acetophenone (p-MINAP) as a primary ligand which is prepared by the action of n-amyl nitrite with p- Methoxy Acetophenone and 1, 10 – Phenanthroline (1, 10-Phen) as a secondary ligand. The Schiff base ligand and its mixed ligand chelate were characterized based on its elemental analysis; IR, 1H NMR, UV-visible spectra, molar conductance and magnetic moment also have been studied.
The mixed ligand complex were found to have the formula [M (p-MINAP)(1, 10-Phen)] and the geometrical structure of the complex were octahedral geometry. The synthesized Schiff base ligand (p-MINAP), 1, 10-Phenanthroline ligand (1, 10-Phen) and their mixed ligand complex were also investigated for their antibacterial and antifungal activity against bacterial species (gram positive bacteria) and (gram negative bacteria) and fungi. The anticancer activities of the newly synthesized compound have been tested against breast (MFC7) and colon (HCT-116) cell lines. The result showed high activity for synthesized compound.
Influence of abiotic factors on diapause termination and temperature requirements for postdiapause development in the European red mite, Panonychus ulmi (Acari: Tetranychidae)
Authors: Martínez-Villar Elena , López-Manzanares Beatriz , Legarrea Saioa , Pérez-Moreno Ignacio , Marco-Mancebón Vicente S. 
Published: December 2023

Abstract

The European red mite Panonychus ulmi (Koch) is widely distributed and it can severely affect pome fruit crops, particularly apple. Pest outbreaks are related to an overuse of non-selective pesticide treatments that lead to the development of resistance and the absence of natural enemies in the orchard. A key aspect to optimize the use of pesticide treatments in the context of IPM is to increase the knowledge on the biology and ecology of the pest to better predict population dynamics and outbreaks. For the European red mite, knowledge on the conditions that lead to diapause breaking by overwintering eggs is essential to model population dynamics. To increase this knowledge, winter eggs were collected during field surveys in northen Spain during three seasons and egg hatching was monitored under controlled temperature and photoperiod conditions in the laboratory. The “number of days exposed to cold temperatures” was the most significant factor that positively affected hatching of overwintering eggs. The time required for 50% of the egg population to hatch (T50%) was also negatively modulated by the duration of exposure to cold temperature. The temperature threshold for postdiapause eggs development collected from the field was estimated between 5 and 6 ºC in 2005 and 2007, respectively. Moreover, the degree-days required for post diapause development were estimated between 255 and 264, depending on the year of collection. Collectively, we provide additional information on the diapause termination and postdiapause development of the European red mite that may effectively contribute to optimize pest population models.
Predictors of quality of work life in health care workers at adult critical care units
Authors: Quiñones-Rozo Laura del Pilar , Canaval-Erazo Gladys Eugenia , Sandoval-Moreno Lina Marcela 
Published: December 2023

Abstract

Background
Some of the health workforce qualified as front-line health care workers work in precarious conditions, which results in inadequate global distribution and quality of health care.
Objective
To identify predictors of the quality of work life of healthcare workers in adult critical care units.
Methods
Quantitative study, cross-sectional analytical design with stratified two-stage sampling; three instruments were applied to 209 healthcare professionals in adult critical care units in different sites in a region of Colombia, concerning Quality of Life at Work - GOHISALO, Copenhagen Psychosocial Questionnaire - COPSOQ and Professional Quality of Life - ProQoL V. Multiple ordinal logistic regression was performed with exposure variables from the COPSOQ and ProQoL domains; the outcome variables were the dimensions of the Quality of Work Life instrument. Ethical standards for research involving human subjects were ensured.
Results
According to the results of the multiple logistic model, quality of work life is predicted by job integration and predictability (OR = 6.93; 95%CI = 3.6–13.9), leisure time management and double presence (OR = 4.5; 95%CI = 1.22–8.79). Both job satisfaction and job security are related to leadership quality (OR = 3.82; 95%CI = 2.27–6.55 and OR = 3.18; 95%CI = 1.22–8.79) respectively.
Conclusions
The quality of work life of healthcare workers in adult intensive care units is predicted by quantitative demands, double presence, emotional demands, work pace, predictability, vertical trust, predictability, and quality of leadership.
Assessing the utility of strontium isotopes in fossil dental calculus
Authors: Dosseto Anthony , Dux Florian , Eisenhofer Raphael , Weyrich Laura 
Published: December 2023

Abstract
Strontium (Sr) isotopes measured in fossil remains have been a useful tool to assess the geographical origin and even migrations of humans and other animals. In particular, dental enamel generally provides as the ideal material, as it is dense and less prone to diagenetic replacement of Sr post-burial. However, fossil teeth can often be precious artefacts and difficult to access for destructive analysis. Here, we assess whether measuring Sr isotopes in fossil dental calculus could be used at least as a rangefinder to determine the geographical origin of an individual. We measured trace element concentrations in modern calculus (from a local dental practice), and trace element concentrations and 87Sr/86Sr ratios in human fossil calculus, dentine and enamel from specimens collected in York, UK. Comparing trace element concentrations between modern and fossil calculus show that metals present in fossil calculus are mostly acquired post-burial, including Sr. The relationship between 87Sr/86Sr and Rb/Sr ratios in fossil calculus, dentine and enamel suggests that the diagenetic endmember would have a 87Sr/86Sr ratio consistent with the one modelled for the York region, but a low Rb/Sr. Without calculus data, dentine and enamel data would have probably suggested a lower 87Sr/86Sr ratio for a diagenetic endmember, expecting high Rb/Sr values. Thus, while Sr isotopes in fossil calculus may not be useful to identify the geographical origin of an individual, they may be useful in constraining the composition of the diagenetic endmember. Combining Sr isotopes in fossil dental calculus and enamel could be a more robust approach to identify geographical origin, than using enamel alone.
Rev. dr. Edmund Nowicki – Apostolic Administrato r in Gorzów Wielkopolski
Authors: Wejman Grzegorz 
Published: December 2023

Rev. PA Edmund Nowicki PhD, a priest of the Archdiocese of Gniezno i Poznań, was strongly associated with Gorzów Wielkopolski through his priestly ministry and with Gdańsk through his episcopal ministry. The work in the apostolic administration in Gorzów Wielkopolski was particularly difficult for him. The enormous area – 1/7 of Poland’s area, the lack of basic religious ministry tools and the difficulties arising from the then authorities posed extremely big problems in creating the pastoral life in the newly created church unit in these lands. More than five years of his work in Western Pomerania and the Lubusz region brought great achievements in the form of a very well-functioning church organization,only permanent church structures were lacking, but nfortunately political factors did not lead to their implementation. This success is the fruit of his fortitude, solid theological and legal background and personal sensitivity. Rev. PA Dr Edmund Nowicki is a great Pole, priest and bishop.