bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uncarboxylated Osteocalcin Inhibits De Novo Lipogenesis and Promotes Fatty Acid Oxidation via SIRT1 to Alleviate Hepatocyte Lipid Accumulation
Authors: Chen Lei , Zhang Miao , Xu Jiaojiao , Yang Jianhong 
Published: February 2024

Abstract

Background Non-alcoholic fatty liver disease (NAFLD) is a disease characterized by the hepatic lipids accumulation resulting from genetic susceptibility and metabolic dysfunction. Uncarboxylated osteocalcin (GluOC) is a protein that is synthesized by osteoblasts which performs a vital function in the management of energy balance. Previous studies have shown that GluOC is beneficial for lipid and glucose metabolism in KKAy mice induced fatty liver. GluOC effectively ameliorates hyperglycemia, fatty liver, and hyperlipidemia. Furthermore, it regulates stearyl-coenzyme A desaturase 1 (SCD1) expression through AMP-activated protein kinase (AMPK), which alleviates hepatocyte lipid accumulation. However, the underlying mechanisms by which GluOC alleviates hepatocyte lipid accumulation need further elucidation. Methods In this study, the NCTC 1469 cells induced by oleic acid (OA) and palmitic acid (PA) were used in the establishment of the NAFLD cell model. Triglyceride (TG) kits and BODIPY 493/503 staining were employed to measure the levels of hepatocyte lipid accumulation. Immunoprecipitation, western blotting, and real-time PCR analyzed the expression of protein and mRNA in the fatty acid oxidation (FAO) and de novo lipogenesis (DNL) pathways. Results The results indicated that increasing concentrations of GluOC resulted in reduced intracellular lipid accumulation and triglyceride levels. GluOC promoted sirtuin1 (SIRT1) expression, inhibited acetyl-CoA carboxylase (ACC) and fatty acid synthetase (FASN) expression, promoted medium-chain acyl-CoA dehydrogenase (MCAD) and long-chain acyl-CoA dehydrogenase (LCAD) expression. In addition, GluOC activated AMPK phosphorylation and peroxisome proliferator activated receptor γ coactivator-1 α (PGC-1α) deacetylation. si-SIRT1 attenuated the above effects of GluOC, resulting in hepatocyte lipid accumulation. Conclusion GluOC inhibited DNL via SIRT1-AMPK and promoted FAO via SIRT1-PGC-1α to alleviate lipid accumulation in hepatocytes. This provides new insights for further research in NAFLD.
Chronic bacterial prostatitis treatment using quadruple regimen therapy: a preliminary prospective observational study
Authors: Hameed Farooq , Anwaar Adeel , mannan Abdul , Hashmi Saadat , Zahoor Muhammad Imran , Anwar Mamoona , Anwar Maria , Saleem Abdullah 
Published: February 2024

Abstract
Background
This study explored the data collected from 27 patients who underwent a new treatment combination of drugs using quadruple regimen therapy with tadafil 5 mg, N-acetylcysteine 200 mg, diclofenac sodium, and a targeted antibiotic for chronic bacterial prostatitis treatment.
Methods
Twenty-seven Pakistani patients underwent a three-glass test. Those who tested positive received quadruple therapy, while negative cases were treated with triple therapy minus antibiotics, using a gradual tapering off technique for a duration of 20 weeks, and followed for 1 year to observe the recurrence of the disease. Patients were evaluated for international prostatic score, improvement in quality of life, uroflowmeteric studies, improvement in symptoms, and disease recurrence. Chi-square test was used to analyze the data, and p value < 0.05 was considered significant.
Results
This study yielded favorable results with significant improvements in symptoms, IPSS score, quality of life, urinary flow rate, and post-micturition residual volume (p < 0.05). Of the 19 participants with positive three-glass tests, 14/19 (73%) saw a complete resolution of symptoms; however, three (26%) had a reemergence six months later. The remaining eight patients (29%) who tested negative did not show the same level of effectiveness as the positive cases, but still observed improvements in quality of life and IPPS score (P < 0.05). However, their symptoms were not fully resolved.
Conclusions
Our study resulted in significant improvement in the patients’ IPSS score, disease symptoms and quality of life after 20 weeks of therapy, with five visits each. All medications were gradually tapered.
Hematological alterations associated with the SNV rs10974944, part of the 46/1 haplotype, in patients from the Brazilian Amazon with BCR::ABL1-negative myeloproliferative neoplasms
Authors: Paes Jhemerson F. , Torres Dania G. , Aquino Deborah C. , Alves Emanuela V. B. , Mesquita Erycka A. , Sousa Miliane A. , Fraiji Nelson Abrahim , Passos Leny N. M. , Abreu Rosângela S. , Silva George A. V. , Tarragô Andréa M. , Mourão Lucivana P. de Souza 
Published: February 2024

Abstract
BCR::ABL1-negative myeloproliferative neoplasms are hematopoietic disorders characterized by panmyelosis. JAK2 V617F is a frequent variant in these diseases and often occurs in the 46/1 haplotype. The G allele of rs10974944 has been shown to be associated with this variant, specifically its acquisition, correlations with familial cases, and laboratory alterations. This study evaluated the association between the 46/1 haplotype of JAK2 in patients with myeloproliferative neoplasms in a population from the Brazilian Amazon. Clinical, laboratory and molecular sequencing analyses were considered. Carriers of the G allele of rs10974944 with polycythemia vera showed an increase in mean corpuscular volume and mean corpuscular hemoglobin, while in those with essential thrombocythemia, there was an elevation in red blood cells, hematocrit, and hemoglobin. Associations were observed between rs10974944and the JAK2 V617F, in which the G allele (OR: 3.47; p < 0.0001), CG genotype (OR: 8.4; p = 0.002), and GG genotype (OR: 4.1; p = 0.002) were associated with JAK2 V617F+ and an increase in variant allele frequency (GG: OR 13.1; p = 0.004; G: OR: 6.0; p = 0.0002). These results suggest an association between rs10974944 (G) and a status for JAK2 V617F, JAK2 V617F+_VAF ≥50%, and laboratory alterations in the erythroid lineage.
Simulation of interfacial debonding in hollow particle-reinforced composites with VCFEM
Authors: Wang Zhiyi , Zhang Rui 
Published: February 2024

Abstract
The addition of hollow glass miscrosphere into composites is a method to improve mechanical properties. However, the interfacial debonding of hollow miscrosphere inevitably causes a decrease in the mechanical properties of the material, which ultimately leads to the failure of the composites. In the numerical simulation of such hollow particle-reinforced composites, the ordinary displacement finite element requires a large number of meshes, which undoubtedly greatly increases the computational cost. In this paper, a new VCFEM is proposed to solve this problem by establishing a two-dimensional Voronoi cell finite element model, deriving the residual energy generalized function of hollow particle-reinforced composites, and calculating the interface debonding. The simulation results are compared with the commercial software MARC, ABAQUS to verify the effectiveness of this VCFEM. The results show that this VCFEM greatly improves the computational efficiency while ensuring the accuracy. Based on this model, this paper also investigates the effect of the generation of interfacial debonding on the overall structure and the effect of different wall thickness of hollow particles on the damage of element debonding.
Implementation of evidence in oral care for adult mechanically ventilated patients with orotracheal intubation using the framework of evidence-based continuous quality improvement: A quality improvement project
Authors: Liu Yanan , Sun Xiaofei , Chen Cancan , Zang Shuting , Zhang Junmei , Zhang Juan , Ren Ying , Wu Hao 
Published: February 2024

Abstract
Background Effective oral care can eliminate bacteria from the oral cavity, enhance oral hygiene, and decrease the occurrence of ventilator-associated pneumonia in mechanically ventilated patients with orotracheal intubation. However, proper implementation of oral care for mechanically ventilated adult patients undergoing orotracheal intubation has not been achieved in clinical practice. The aim was to examine the effectiveness of implementing the evidence in oral care for adult mechanically ventilated patients with orotracheal intubation using the framework of evidence-based continued quality improvement and to take actions to improve nursing quality.Methods Pre- and post-implementation studies were carried out employing a framework of evidence-based continuous quality improvement at the comprehensive intensive care unit of Henan Provincial People’s Hospital in Zhengzhou, China from January to December 2022. A baseline survey was conducted to analyse barriers and facilitators to evidence implementation, informing the development of support strategies such as training nursing staff and creating resources, system flow improvements, and tools. After the evidence implementation, changes in nurses, patients, and the system were assessed. The statistical analysis of the data was performed using SPSS 26.0.Results Nurses' knowledge scores and compliance with evidence significantly improved. The modified Beck Oral Assessment Score decreased from (16.28 ± 1.02) to (8.52 ± 0.45). The detection rate of oral sputum pathogens decreased from 50.0–24.0%. Standardized oral care and workflows for mechanically ventilated adult patients undergoing orotracheal intubation were successfully established.Conclusion The framework of evidence-based continuous quality improvement facilitated the successful implementation of evidence in oral care for adult mechanically ventilated patients with orotracheal intubation. This resulted in improved knowledge among nurses and enhanced oral hygiene for patients, ultimately contributing to the ongoing improvement of nursing quality.
Impact of Timing on Lower Extremity Amputations in Blunt Polytrauma : A Retrospective Analysis of Clinical Outcomes and Risk Factors
Authors: Kim Jinjoo , Choi Wanseon , Cho Wontae , Moon Jonghwan 
Published: February 2024

Abstract
Delayed amputation after failed limb salvage can lead to negative clinical and functional outcomes due to complications, including re-amputation. This study aimed to compare clinical outcomes and identify risk factors according to the timing of amputation. A retrospective review of managed lower-extremity injuries was conducted between January 2016 and December 2022 at a level 1 trauma center. Outcomes were compared between the early amputation and the delayed amputation groups (within and beyond 48 h after the time of injury, respectively). The primary outcome of interest was that the risk factors changed to a more proximal amputation level according to the timing of amputation. The secondary outcomes included trauma-related complications and clinical outcomes. The incidence of trauma-related complications was more common in the delayed amputation group with no significant differences in 30-day mortality. Delayed amputation and stump site deep surgical infection were associated with higher odds of changing to a more proximal level than initially predicted. The overall hospital stay was significantly longer in the delayed amputation group. Although patients and clinicians prefer the limb salvage strategy, delayed amputation could be a risk factor for shorter limb stumps due to re-amputation, and patients may experience more trauma-related complications and prolonged hospitalization.
Farmers’ knowledge and attitude towards the use of low-cost stationary brush for dairy cows
Authors: Nascimento Fernanda Gatti de Oliveira , Brito Amanda Aparecida , Bóscollo Paola Perez , Brettas Patrícia Kelly de Moraes , França André Madeira Silveira , De-Sousa Karolini Tenffen , Faria Priscila Neves , Guimarães Ednaldo Carvalho , Nascimento Mara Regina Bueno de Mattos 
Published: February 2024

Abstract
The aim was to assess farmers’ knowledge of herd behaviour, grooming-related injuries, stress reduction, and familiarity with a brush device, and their attitude in adopting a low-cost stationary brush. Farmers from Southeast and Midwest of Brazil participated in an online survey. The questionnaire was organized into sections: first about the profile of farmers, second focused on the description of milk production system, and third explore farmers' knowledge and attitude towards brush devices. The data were analyzed using descriptive statistics, and a multiple logistic regression model estimated the odds ratio. Farmers with a higher level of milking system technification were found to have 2.85 times greater odds of recognizing the need to reduce stress. Farmers interested in a low-cost brush were found to have 10.48 times greater odds of recognizing the need for stress-reducing improvements. Those who believe that the use of a brush improves milk production and cow welfare have 14.15 times higher odds of installing the low-cost brush. Most farmers reported familiarity with cow brushes. Moreover, farmers recognize the use of brushes as a beneficial strategy for reducing stress, improving milk production, and enhancing animal welfare, Consequently, they express willingness to install a low-cost stationary brush for dairy cows.
Validity of annealed approximation in a high-dimensional system
Authors: Um Jaegon , Hong Hyunsuk , Park Hyunggyu 
Published: February 2024

Abstract
This study investigates the suitability of the annealed approximation in high-dimensional systems characterized by dense networks with quenched link disorder, employing models of coupled oscillators. We demonstrate that dynamic equations governing dense-network systems converge to those of the complete-graph version in the thermodynamic limit, where link disorder fluctuations vanish entirely. Consequently, the annealed-network systems, where fluctuations are attenuated, also exhibit the same dynamic behavior in the thermodynamic limit. However, a significant discrepancy arises in the incoherent (disordered) phase wherein the finite-size behavior becomes critical in determining the steady-state pattern. To explicitly elucidate this discrepancy, we focus on identical oscillators subject to competitive attractive and repulsive couplings. In the incoherent phase of dense networks, we observe the manifestation of random irregular states. In contrast, the annealed approximation yields a symmetric (regular) incoherent state where two oppositely coherent clusters of oscillators coexist, accompanied by the vanishing order parameter. Our findings imply that the annealed approximation should be employed with caution even in dense-network systems, particularly in the disordered phase.
Integrative analysis of transcriptome and metabolome profiling reveal coloration molecular mechanisms in four rapeseed petals with different color
Authors: Zheng Tao , Chen Qiao , Chen Mengjiao , Zeng Haitao , Xue Yan , Tang Qi , Xu Hao , Li Ying 
Published: February 2024

Abstract

Background
The colorful rapeseed petals have specific phenotype and excellent ornamental value. However, the coloration mechanism of rapeseed petals are poorly understood. In this study, the integrative metabolome and transcriptome analysis of white, yellow, dark purple, and dark red rapeseed petals were analyzed to elucidate the mechanisms regulating petal pigmentation.
Results
The metabolome analysis results revealed that chalcones compounds, tamarixetin-3-O-glucoside-7-O-rhamnoside and 4'-hydroxy-2,4,6-trimethoxydihydrochalcone diaplayed a higher accumulation in the yellow color of rape petals, acylated anthocyanins, cyanidin-3-O- (6"-O-caffeoyl) sophoroside-7-O-glucoside and cyanidin-3-O-feruloylglucoside-5-O-glucoside were the main pigments that caused the dark purple color of rapeseed petals, and glycosylated anthocyanins, peonidin-3-O-glucoside were the main coloring substances in the dark red rape petals. KEGG and qRT-PCR results suggested that the higher expression of CHS lead to the accumulation of 4'-hydroxy-2,4,6-trimethoxydihydrochalcone in yellow petals. ANS1, CCoAAT2 and CCoAOMT were responsible for regulating the biosynthesis of cyanidin-3-O- (6"-O-caffeoyl) sophoroside-7-O-glucoside and cyanidin-3-O-feruloylglucoside-5-O-glucoside in dark purple petals. ANS1, UGT, MT1, and MT2 co-expressed to regulate peonidin-3-O-glucoside content in dark red petals. The differential expression of CHS, ANS, UGT, CCoAOMT, CCoAAT2, and MT2 was contributed to the yellow, dark purple and dark red formation of rapeseed flowers. Through weighted gene co-expression network analysis, it was found that five modules were highly correlated with flavonoids accumulation, in which five candidate transcription factors MYB12, MYB122, MYB114, bHLH21, and bHLH96 related to flavonoids biosynthesis were identified.
Conclusion
A genetic and metabolic regulatory network of flavonoid biosynthesis in rapeseed petals with different color was proposed, according to the those differential flavonoids and the candidate genes screened. In summary, this study elucidated the molecular mechanism of coloration formation in rapeseed petal for comprehensive utilization.
Mining and analysis of adverse event signals of Mometasone furoate based on real-world data from the FAERS database
Authors: Zou Zhixin , Long Ziyi , Zhang Qidi , Chen Jianjun 
Published: February 2024

Abstract
Background
Mometasone furoate (MF) is a steroidal active pharmaceutical ingredient (API) that has been approved for the treatment of atopic dermatitis, eczema, allergic rhinitis, and chronic rhinosinusitis by the FDA. The aim of the present study was to assess real-world MF-related adverse events (AEs) through data mining of the US Food and Drug Administration Adverse Event Reporting System (FAERS).
Methods
Disproportionality analyses, including the reporting odds ratio (ROR), the proportional reporting ratio (PRR), the Bayesian confidence propagation neural network (BCPNN) and the multi-item gamma Poisson shrinker (MGPS) algorithms, were employed to quantify the signals of MF-associated AEs.
Results
Out of 49,659,292 reports collected from the FAERS database, 11,510 reports of MF-associated AEs were identified. A total of 185 significant preferred terms (PTs) conforming to the four algorithms were retained. The most common AEs included epistaxis, nasal dryness, burning, stinging, blood-tinged secretions, pharyngeal inflammation, skin weeping and pruritis allergic reactions, which corresponded to those reported in the specification and clinical trials. Unexpected significant AEs, such as chorioretinopathy, retinal edema, ageusia, anosmia, otorrhea, ear pruritus, auricular swelling and limb reduction defects, might also occur.
Conclusion
The results of our study were consistent with clinical observations, and we also found potential new and unexpected AEs associated with MF. Prospective clinical studies are needed to confirm these results and determine their relationship. Our results could provide valuable evidence for further safety studies of MF.
The impact of time perception on consumer’s intertemporal choices: a study on self-improvement and self-enhancement products
Authors: Hou Min , Yan Linlin , Gu Chunmei 
Published: February 2024

Abstract

Consumer decisions often have both short- and long-term consequences, making Subjective Time Perception an important variable in intertemporal choice research. However, limited studies have explored the relationship between the subjective time perception and intertemporal choices in specific product contexts. To gain a deeper understanding of consumer behavior, it is crucial to examine how subjective time perception (STP) impacts the decision-making process. This study focuses on subjective time perception and its effect on intertemporal choices, while also considering the moderating role of product type (self-improvement vs. self-enhancement). Study 1 shows that individuals with a shorter sense of STP are more inclined to choose delayed rewards. Study 2 further confirms that consumers make different intertemporal choices based on the product type. For self-improvement products, individuals with a shorter STP are more likely to choose delayed rewards, whereas those with a longer STP are more inclined to choose immediate rewards. On the other hand, for self-enhancement products, there was no difference in intertemporal choices. Lastly, Study 3 reveals that the way individuals perceive time plays a significant moderating role in how product type influences intertemporal choices. The research findings confirm previous evidence on the important role of time perception in intertemporal choices under different product contexts.
Significant efficacy of conversion therapy for advanced hepatocellular carcinoma: a case report
Authors: Fang Cheng , Zheng Rujun , Guo Qinxi , Li Bo , Su Song 
Published: February 2024

Abstract
Hepatocellular carcinoma (HCC) is the sixth most common cancer and the third leading cause of cancer-related mortality worldwide [1]. This study aimed to report a case with advanced-stage HCC who underwent transhepatic arterial chemotherapy and embolization combined with anlotinib, sintilimab, and radiotherapy. He successfully received conversion therapy to get tumor downstaging and finally had an opportunity to undergo a right lobe hepatectomy. The postoperative pathological examination confirmed a pathologic complete response, and the level of serum alpha-fetoprotein decreased from > 1000 to 11.33 ng/mL. The follow-up to date revealed that the progression-free survival time was more than 13 months. It indicated that combined therapy might be a superior choice for the conversion therapy of patients with advanced-stage HCC.
Biofilm-disrupting effects of phage endolysins LysAm24, LysAp22, LysECD7, and LysSi3: breakdown the matrix
Authors: Lendel Anastasiya M. , Antonova Nataliia P. , Grigoriev Igor V. , Usachev Evgeny V. , Gushchin Vladimir A. , Vasina Daria V. 
Published: February 2024

Abstract

The ability of most opportunistic bacteria to form biofilms, coupled with antimicrobial resistance, hinder the efforts to control widespread infections, resulting in high risks of negative outcomes and economic costs. Endolysins are promising compounds that efficiently combat bacteria, including multidrug-resistant strains and biofilms, without the subsequent emergence of endolysin-resistant genotypes. However, the details of antibiofilm effects of these enzymes are poorly understood. To elucidate the interactions of bacteriophage endolysins LysAm24, LysAp22, LysECD7, and LysSi3 with bacterial films formed by Gram-negative species, we estimated their composition and assessed the endolysins’ effects on the most abundant exopolymers in vitro. The obtained data suggests a pronounced efficiency of these lysins against biofilms with high (Klebsiella pneumoniae) and low (Acinetobacter baumannii) matrix contents, or dual-species biofilms, resulting in at least a 2-fold loss of the biomass. These peptidoglycan hydrolases interacted diversely with protective compounds of biofilms such as extracellular DNA and polyanionic carbohydrates, indicating a spectrum of biofilm-disrupting effects for bacteriolytic phage enzymes. Specifically, we detected disruption of acid exopolysaccharides by LysAp22, strong DNA-binding capacity of LysAm24, both of these interactions for LysECD7, and neither of them for LysSi3.