bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diffusing capacity as an independent predictor of acute exacerbations in chronic obstructive pulmonary disease
Authors: Park Heemoon , Lee Hyo Jin , Lee Jung-Kyu , Park Tae Yun , Jin Kwang Nam , Heo Eun Young , Kim Deog Kyeom , Lee Hyun Woo 
Published: February 2024

AbstractA weak correlation between diffusing capacity of the lung for carbon monoxide (DLCO) and emphysema has been reported. This study investigated whether impaired DLCO in chronic obstructive pulmonary disease (COPD) is associated with increased risk of acute exacerbation independent of the presence or extent of emphysema. This retrospective cohort study included patients with COPD between January 2004 and December 2019. The participants were divided into four groups based on visually detected emphysema and impaired DLCO. Among 597 patients with COPD, 8.5% had no emphysema and impaired DLCO whereas 36.3% had emphysema without impaired DLCO. Among the four groups, patients with impaired DLCO and emphysema showed a higher risk of moderate-to-severe or severe exacerbation than those with normal DLCO. Impaired DLCO was an independent risk factor for severe exacerbation (hazard ratio, 1.524 [95% confidence interval 1.121–2.072]), whereas the presence of emphysema was not. The risk of moderate-to-severe or severe exacerbation increases with the severity of impaired DLCO. After propensity-score matching for the extent of emphysema, impaired DLCO was significantly associated with a higher risk of moderate-to-severe (p = 0.041) or severe exacerbation (p = 0.020). In patients with COPD and heterogeneous parenchymal abnormalities, DLCO can be considered an independent biomarker of acute exacerbation.
Effects of irrigation scheduling on the yield and irrigation water productivity of cucumber in coconut coir culture
Authors: Li You-li , Zhang Si-qi , Guo Wen-zhong , Zheng Wen-gang , Zhao Qian , Yu Wen-ya , Li Jian-she 
Published: February 2024

AbstractOptimum irrigation scheduling is important for ensuring high yield and water productivity in substrate-cultivated vegetables and is determined based on information such as substrate water content, meteorological parameters, and crop growth. The aim of this study was to determine a precise irrigation schedule for coconut coir culture in a solar greenhouse by comparing the irrigation, evapotranspiration (ET), substrate water content (VWC), as well as the crop growth indices and yield of cucumber, and irrigation water productivity (IWP) under three irrigation schedules: the soil moisture sensor-based method (T-VWC), the accumulated radiation combined with soil moisture sensor-based method (Rn-VWC), and the crop evapotranspiration estimated method using the hourly PM-ETo equation with an improved calculation of Kc (T-ETc). The results showed that the daily irrigation and evapotranspiration amount were the highest under T-VWC treatment, while the lowest under T-ETc treatment. In different meteorological environments, the change in irrigation amount was more consistent with the ET,and the VWC was relatively stable in T-ETc treatment compared with that under T-VWC or Rn-VWC treatments. The plant height, leaves number, leaf area, and stem diameter of T-VWC and Rn-VWC treatments were higher than those of the T-ETc treatments, but there was no significant difference in cucumber yield. Compared with the T-VWC treatment, total irrigation amount under Rn-VWC and T-ETc treatments significantly decreased by 25.75% and 34.04%, respectively ($$\hbox {P}<0.05$$

P
<
0.05

). The highest IWP values of 25.07 kg m$$^{-3}$$

-
3

was achieved from T-ETc treatment with significantly increasing by 44.33% compared to the T-VWC treatment (17.37 kg m$$^{-3}$$

-
3

). In summary, the T-ETc treatment allowed more reasonable irrigation management and was appropriate for growing cucumber in coconut coir culture.
Static micromechanical measurements of the flexural modulus and strength of micrometre-diameter single fibres using deflecting microcantilever techniques
Authors: Reda Ali , Arscott Steve 
Published: February 2024

AbstractThe mechanical properties of natural and man-made fibres ultimately govern the robustness of products. Examples range from textiles to composite materials for mechanical parts in emerging technological applications. An accurate determination of the mechanical properties of microscopic single fibres is therefore important. Today, macroscopic mechanical techniques, such as tensile testing, are commonly employed to obtain this information. However, a relatively high dispersion of results is often encountered due to a relatively long sample size. As an alternative to tensile methods, we demonstrate here micromechanical techniques to accurately measure the flexural modulus and strength of micrometre-sized diameter fibres without the need of force sensing. To demonstrate our ideas, we use the example of single natural fibres (Linum Usitatissimum). The flexural modulus of the single fibres is first accurately measured in the low deflection regime of an inclined bending cantilever in an original setup. The flexural strength of the single fibres is then measured in the high deflection regime of a bending cantilever. Interestingly, the novel measurements have allowed the authors to quantify the flexural strength of two different failure modes in flax fibre, enabling a contribution to plant mechanics.
Uncontrolled blood pressure and its risk factors among hypertensive patients, Marrakech, Morocco
Authors: Belayachi Safae , Boukhari Fatima Zahra , Essayagh Firdaous , Terkiba Othmane , Marc Ikram , Lambaki Abdellah , Zohoun Alban , Essayagh Meriem , Essayagh Touria , Essayagh Sanah 
Published: February 2024

AbstractHypertension is a public health problem. Failure to control blood pressure figures is responsible for morbidity and premature mortality. This study aims to describe the characteristics of hypertensive patients followed at primary health care centers in Marrakech. Between May 2021 and December 2022, a cross-sectional study of 922 hypertension patients attending primary health care centers in Marrakech was done. To gather socio-demographic, behavioral, and clinical data, as well as hypertension treatment features and the care-patient-physician triad, a face-to-face questionnaire was employed. To identify risk factors associated with uncontrolled blood pressure, multivariate logistic regression was used. Uncontrolled blood pressure was found in 73.5% of people. The patients' average age was 63.4 ± 9.4 years (mean ± standard deviation), and 524 (77.3%) were women. Tobacco consumption (Adjusted Odd Ratio of 4.34; 95% CI [1.58–11.9]); lack of self-monitoring of hypertension (AOR of 1.69; 95% CI [1.14–2.52]); a family history of hypertension (AOR of 1.58; 95% CI [1.12–2.22]); overweight or obesity (AOR of 1.73; 95% CI [1.15–2.58]); and nonadherence to antihypertensive medication (AOR of 1.58; 95% CI [1.05–2.38]) were identified as risk factors for uncontrolled blood pressure. In hypertensive individuals, the percentage of uncontrolled blood pressure is considerable. It is essential to provide therapeutic education classes for hypertension patients in order to strengthen their power and autonomy in managing their hypertension.
Advanced strain elastography is a reliable approach for prostate cancer detection in patients with elevated PSA levels
Authors: Almalki Yassir Edrees , Mansour Mohamed Gamal El-Din , Ali Susan Adil , Basha Mohammad Abd Alkhalik , Abdelkawi Moustafa Mahmoud , Alduraibi Sharifa Khalid , Almushayti Ziyad A. , Aldhilan Asim S. , Aboualkheir Mervat , Amin Darine , Metkees Mohamed , Basha Ahmed M. A. , Ebaid Noha Yahia 
Published: February 2024

AbstractThis study aimed to examine the validity and reproducibility of strain elastography (SE) for detecting prostate cancer (PCa) in patients with elevated prostate-specific antigen (PSA) levels. The study included 107 patients with elevated PSA levels. All eligible patients underwent transrectal ultrasound (TRUS) with real-time elastography (RTE) to detect suspicious lesions. Two readers independently evaluated the lesions and assigned a strain ratio and elastography score to each lesion. Histopathology was used as a reference standard to estimate the validity of RTE in predicting malignant lesions. An intraclass correlation (ICC) was performed to detect reliability of the strain ratios and elastography scores. TRUS-guided biopsy detected malignancies in 64 (59.8%) patients. TRUS with RTE revealed 122 lesions. The strain ratio index (SRI) cut-off values to diagnose malignancy were 4.05 and 4.35, with sensitivity, specificity, and accuracy of 94.7%, 91.3%, and 93.4%, respectively. An elastography score > 3 was the best cut-off value for detecting malignancy. According to readers, the sensitivity, specificity, and accuracy were 91.3–94.7%, 89.5–93.4%, and 91.3–90.9%, respectively. Excellent inter-reader agreement was recorded for SRI and elastography scores, with ICC of 0.937 and 0.800, respectively. SE proves to be an efficient tool for detecting PCa with high accuracy in patients with elevated PSA levels.
Diagnostic value of renal biopsy in anti-phospholipase A2 receptor antibody-positive patients with proteinuria in China
Authors: Lu Shan , Xiao Jing , Liu Dong , Zhang Yan , Dong Yijun , Zhao Zhanzheng 
Published: February 2024

AbstractRenal biopsy remains the gold standard for diagnosing membranous nephropathy (MN). Recent studies have suggested that renal biopsy can be replaced with the serum phospholipase A2 receptor (PLA2R) antibody test for MN diagnosis in patients with nephrotic syndrome. However, this test has not been validated in the Chinese population. In this study, we investigated whether renal biopsy provides additional diagnostic information on patients with proteinuria who are seropositive for PLA2R antibodies (SAb +). We retrospectively reviewed the clinicopathological characteristics of SAb + adult patients (aged ≥ 18 years) with proteinuria (≥ 0.5 g/24 h) assessed at the Department of Nephrology, the First Affiliated Hospital of Zhengzhou University, from June 2021 to March 2022. Among a total of 801 SAb + patients who received renal biopsy, those with incomplete pathological data, diabetes or any potential cause of secondary MN were excluded. Among the 491 remaining patients, 474 had primary MN (PMN), 16 had atypical MN (AMN, 9 patients with “full house” and 2 patients with HBsAg + /HBcAg + immunofluorescence results), and 1 had focal segmental glomerulosclerosis. In patients with an eGFR of ≥ 60 mL/min/1.73 m2 (n = 451), 436 had PMN, and 71 (16.3%) exhibited additional biopsy findings, with obesity-related glomerulopathy being the most common. In patients with an impaired eGFR (n = 40), 38 had PMN, and 31 (81.6%) showed additional findings, with acute tubular injury being the most common. In conclusion, anti-PLA2R antibody positivity is highly predictive of PMN in Chinese adults but often coexists with other pathological diagnoses. The advantages of renal biopsy for detecting other pathologies should be weighed against the potential risks of the biopsy procedure.
Comparison of noninvasive cardiac output and stroke volume measurements using electrical impedance tomography with invasive methods in a swine model
Authors: Chung Chi Ryang , Ko Ryoung Eun , Jang Geuk Young , Lee Kyounghun , Suh Gee Young , Kim Yongmin , Woo Eung Je 
Published: February 2024

AbstractPulmonary artery catheterization (PAC) has been used as a clinical standard for cardiac output (CO) measurements on humans. On animals, however, an ultrasonic flow sensor (UFS) placed around the ascending aorta or pulmonary artery can measure CO and stroke volume (SV) more accurately. The objective of this paper is to compare CO and SV measurements using a noninvasive electrical impedance tomography (EIT) device and three invasive devices using UFS, PAC-CCO (continuous CO) and arterial pressure-based CO (APCO). Thirty-two pigs were anesthetized and mechanically ventilated. A UFS was placed around the pulmonary artery through thoracotomy in 11 of them, while the EIT, PAC-CCO and APCO devices were used on all of them. Afterload and contractility were changed pharmacologically, while preload was changed through bleeding and injection of fluid or blood. Twenty-three pigs completed the experiment. Among 23, the UFS was used on 7 pigs around the pulmonary artery. The percentage error (PE) between COUFS and COEIT was 26.1%, and the 10-min concordance was 92.5%. Between SVUFS and SVEIT, the PE was 24.8%, and the 10-min concordance was 94.2%. On analyzing the data from all 23 pigs, the PE between time-delay-adjusted COPAC-CCO and COEIT was 34.6%, and the 10-min concordance was 81.1%. Our results suggest that the performance of the EIT device in measuring dynamic changes of CO and SV on mechanically-ventilated pigs under different cardiac preload, afterload and contractility conditions is at least comparable to that of the PAC-CCO device. Clinical studies are needed to evaluate the utility of the EIT device as a noninvasive hemodynamic monitoring tool.
Preference for diagnosing and treating renal colic during pregnancy: a survey among Chinese urologists
Authors: Deng Shidong , Guo Dayong , Liu Lingzhi , Wang Yurou , Fei Kuilin , Zhang Huihui 
Published: February 2024

AbstractTo explore the preference for diagnosing and treating renal colic during pregnancy among Chinese urologists. A questionnaire was designed using the Sojump® platform. WeChat, the largest social networking platform in China, was used to distribute the questionnaire to urologists at hospitals of all levels in China. In total, 110 responses were included. Of the respondents, 100.0% used ultrasound to diagnose renal colic during pregnancy, followed by magnetic resonance imaging (17.3%) and low-dose CT (3.6%). Phloroglucinol (80.9%) and progesterone (72.7%) were the most commonly used antispasmodics and analgesics. Opioid analgesics were not commonly used (12.7%). Most of the respondents (63.6%) indicated that no more than 20% of the patients needed surgical intervention. If surgery was unavoidable, 95.5% preferred temporary renal drainage, including ureteral stenting (92.7%) and percutaneous nephrostomy (2.7%). However, some respondents still preferred definitive stone treatment, such as ureteroscopy lithotripsy (3.6%) and percutaneous nephrolithotomy (0.9%). Moreover, there were no differences in the choices of urologists with different professional titles regarding diagnostic tools, most therapeutic medications, or surgical methods (p > 0.05). Ultrasound is the preferred tool for diagnosing renal colic during pregnancy. Low-dose CT is still not widely accepted. Pregnant patients with renal colic are initially treated conservatively. Urologists prefer ureteral stenting when there are clinical indications for intervention.
Chemical control of excited-state reactivity of the anionic green fluorescent protein chromophore
Authors: List Nanna H. , Jones Chey M. , Martínez Todd J. 
Published: February 2024

AbstractControlling excited-state reactivity is a long-standing challenge in photochemistry, as a desired pathway may be inaccessible or compete with other unwanted channels. An important example is internal conversion of the anionic green fluorescent protein (GFP) chromophore where non-selective progress along two competing torsional modes (P: phenolate and I: imidazolinone) impairs and enables Z-to-E photoisomerization, respectively. Developing strategies to promote photoisomerization could drive new areas of applications of GFP-like proteins. Motivated by the charge-transfer dichotomy of the torsional modes, we explore chemical substitution on the P-ring of the chromophore as a way to control excited-state pathways and improve photoisomerization. As demonstrated by methoxylation, selective P-twisting appears difficult to achieve because the electron-donating potential effects of the substituents are counteracted by inertial effects that directly retard the motion. Conversely, these effects act in concert to promote I-twisting when introducing electron-withdrawing groups. Specifically, 2,3,5-trifluorination leads to both pathway selectivity and a more direct approach to the I-twisted intersection which, in turn, doubles the photoisomerization quantum yield. Our results suggest P-ring engineering as an effective approach to boost photoisomerization of the anionic GFP chromophore.
Effect of vessel compression on blood flow in microvascular networks and its implications for tumour tissue hypoxia
Authors: Enjalbert Romain , Krüger Timm , Bernabeu Miguel O. 
Published: February 2024

AbstractThe tumour microenvironment is abnormal and one of its consequences is that blood vessels are compressed. Vessel compression correlates with reduced survival rates, while decompression of vessels improves tissue oxygenation as well as increases survival rates. Vessel compression contributes, at a single vascular bifurcation, to the increase of heterogeneity of red blood cell (RBC) transport. However, the effect that vessel compression has at a network level is unknown. This work numerically investigates the effect of vessel compression on RBC transport in microvascular networks. The key findings are that vessel compression both reduces the average haematocrit, and increases haematocrit heterogeneity, in vessels in the network. The mechanisms for these changes in haematocrit distribution are unravelled, and a parameter sweep shows that networks with lower inlet haematocrits are more susceptible to haemodilution from vessel compression over a wide range of compressed fraction of a network. These findings provide a theoretical underpinning for the link between vessel compression and tumour tissue hypoxia.
A GIPR antagonist conjugated to GLP-1 analogues promotes weight loss with improved metabolic parameters in preclinical and phase 1 settings
Authors: Véniant Murielle M. , Lu Shu-Chen , Atangan Larissa , Komorowski Renee , Stanislaus Shanaka , Cheng Yuan , Wu Bin , Falsey James R. , Hager Todd , Thomas Veena A. , Ambhaikar Malhar , Sharpsten Lucie , Zhu Yineng , Kurra Vamsi , Jeswani Rohini , Oberoi Rajneet K. , Parnes Jane R. , Honarpour Narimon , Neutel Joel , Strande Jennifer L. 
Published: February 2024

AbstractObesity is a major public health crisis. Multi-specific peptides have emerged as promising therapeutic strategies for clinical weight loss. Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are endogenous incretins that regulate weight through their receptors (R). AMG 133 (maridebart cafraglutide) is a bispecific molecule engineered by conjugating a fully human monoclonal anti-human GIPR antagonist antibody to two GLP-1 analogue agonist peptides using amino acid linkers. Here, we confirm the GIPR antagonist and GLP-1R agonist activities in cell-based systems and report the ability of AMG 133 to reduce body weight and improve metabolic markers in male obese mice and cynomolgus monkeys. In a phase 1, randomized, double-blind, placebo-controlled clinical study in participants with obesity (NCT04478708), AMG 133 had an acceptable safety and tolerability profile along with pronounced dose-dependent weight loss. In the multiple ascending dose cohorts, weight loss was maintained for up to 150 days after the last dose. These findings support continued clinical evaluation of AMG 133.
Realtime monitoring of thrombus formation in vivo using a self-reporting vascular access graft
Authors: Hoare Daniel , Kingsmore David , Holsgrove Michael , Russell Ewan , Kirimi Mahmut T. , Czyzewski Jakub , Mirzai Nosrat , Kennedy Simon , Neale Steven L. , Mercer John R. 
Published: February 2024

Abstract
Background
Chronic kidney disease (CKD) affects 10% of the global population costing over a hundred billion dollars per annum and leading to increased risk of cardiovascular disease. Many patients with CKD require regular haemodialyses. Synthetic arteriovenous grafts (AVG) are increasingly used to provide rapid vascular connection for dialysis. Initially, they have excellent patency rates but are critically limited by neointimal hyperplasia at the venous anastomosis, which drives subsequent thrombosis, graft failure and death.

Methods
Here, we describe a system in which electrical impedance spectroscopy sensors are incorporated circumferentially into the wall of a synthetic arteriovenous graft. This is combined with an implantable radiotelemetry system for data transmission outside the patient. The system was tested using monolayers of endothelial and smooth muscle cells as well as swine blood and clots with explanted human carotid artery plaques. Sensor testing was then performed in vitro and the device was implanted in vivo in female swine.

Results
The device can wirelessly report the accumulation of biological material, both cells and blood. Differences are also detected when comparing controls with pathological atheroma. In swine differences between blockage formation in a graft were remotely obtained and wireless reported.

Conclusions
Combining electrical impedance spectroscopy and an implantable radiotelemetry system enables graft surveillance. This has the potential to be used for early detection of venous stenosis and blood clot formation in real-time in vivo. In principle, the concept could apply to other cardiovascular diseases and vascular implantable devices.
The DDHD2-STXBP1 interaction mediates long-term memory via generation of saturated free fatty acids
Authors: Akefe Isaac O , Saber Saber H , Matthews Benjamin , Venkatesh Bharat G , Gormal Rachel S , Blackmore Daniel G , Alexander Suzy , Sieriecki Emma , Gambin Yann , Bertran-Gonzalez Jesus , Vitale Nicolas , Humeau Yann , Gaudin Arnaud , Ellis Sevannah A , Michaels Alysee A , Xue Mingshan , Cravatt Benjamin , Joensuu Merja , Wallis Tristan P , Meunier Frédéric A 
Published: February 2024

AbstractThe phospholipid and free fatty acid (FFA) composition of neuronal membranes plays a crucial role in learning and memory, but the mechanisms through which neuronal activity affects the brain’s lipid landscape remain largely unexplored. The levels of saturated FFAs, particularly of myristic acid (C14:0), strongly increase during neuronal stimulation and memory acquisition, suggesting the involvement of phospholipase A1 (PLA1) activity in synaptic plasticity. Here, we show that genetic ablation of the PLA1 isoform DDHD2 in mice dramatically reduces saturated FFA responses to memory acquisition across the brain. Furthermore, DDHD2 loss also decreases memory performance in reward-based learning and spatial memory models prior to the development of neuromuscular deficits that mirror human spastic paraplegia. Via pulldown-mass spectrometry analyses, we find that DDHD2 binds to the key synaptic protein STXBP1. Using STXBP1/2 knockout neurosecretory cells and a haploinsufficient STXBP1+/− mouse model of human early infantile encephalopathy associated with intellectual disability and motor dysfunction, we show that STXBP1 controls targeting of DDHD2 to the plasma membrane and generation of saturated FFAs in the brain. These findings suggest key roles for DDHD2 and STXBP1 in lipid metabolism and in the processes of synaptic plasticity, learning, and memory.