bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Binding Occupancy of Pro-Hypertrophic Transcription Factors among Cardiac Developmental Enhancers
Authors: Zehra Raza Rabail , Hussain Irfan , Akhtar Fatima , Mumtaz Sara 
Published: January 2024

Objective: To check which of the eight pro-hypertrophic transcription factors bind in abundance to a set of 140 functionally confirmed cardiac enhancers aiming to identify potential therapeutic targets for delaying the onset of cardiac hypertrophy. Study Design: Bioinformatics, in-Silico Study.Place and Duration of Study: The study was conducted at the Department of Biological Sciences, National University of Medical Sciences (NUMS), Rawalpindi, Pakistan, from January 2022 to December 2022.Methods: For experimentally verified human and mouse noncoding regions with gene enhancer activity measured in transgenic mice, VISTA Enhancer Browser was used to obtain 140 cardiac-specific functionally confirmed enhancers. Among these enhancers, transcription factor binding sites were computationally mapped to assess transcription factor occupancy. The binding sites for all eight transcription factors were screened in the collective set of 140 cardiac enhancers via an in-house script. Results: In this study, among a small set of eight pro-hypertrophic transcription factors, occupancy of transcription factor HAND (Heart- And Neural Crest Derivatives-Expressed Protein) was found to be maximum in the collected pool of functionally confirmed cardiac enhancers. Conclusion: The transcription factor HAND was found to have maximum binding occupancy among a set of experimentally confirmed cardiac enhancers.How to cite this: Raza RZ, Hussain I, Akhtar F, Mumtaz S. The Binding Occupancy of Pro-Hypertrophic Transcription Factors among Cardiac Developmental Enhancers. Life and Science. 2024; 5(1): 22-26. doi: http://doi.org/10.37185/LnS.1.1.464
Role of Prophylactic Antibiotics in Preventing Stroke-Associated Pneumonia in Patients with Ischemic Stroke
Authors: Aslam Muhamad Jawad , Amir Muhammad , Sayed Tahir Mukhtar , Hashmat Asif , Hussain Maryam , Fatima Minahil 
Published: January 2024

Objective: To compare the frequency of stroke-associated pneumonia in patients given prophylactic antibiotics versus those not given prophylactic antibiotics.Study Design: Comparative Cross-sectional study.Place and Duration of Study: The study was carried out at the Department of Internal Medicine, Combined st st Military Hospital (CMH), Peshawar, Pakistan from 1 August 2022 to 31 January 2023.Methods: We included 250 ischemic stroke patients and divided them into groups A, “Prophylactic group,” and B, “no prophylactic antibiotics group.” Patients were assessed at 7 days after admission for presence of “stroke associated pneumonia (SAP)” through clinical examination and chest X-rays. Data were analyzed using a statistical package for social sciences 22. For qualitative variables, frequency and percentages were used, whereas for quantitative data, the median (IQR) was used as the data were not normally distributed and contained outliers; using the median and IQR can be more appropriate for describing the central tendency and spread of the data compared to the mean and standard deviation.Results: In our study, the median age of the patients was 68(46-83) years. 61 (24.40%) of the patients were male while 189(75.60%) were female. The median BMI was 31 (19-44) kg/m2. Diabetes was present in 123 (49.20%) patients and 96(38.40%) had hypertension. We found that in the the “prophylactic antibiotics” group (A), 9 (7.20%) patients developed SAP, while in the “no prophylactic antibiotics” group (B) frequency of SAP was 34 (27.20%) (p< 0.001).Conclusion: Prophylactic antibiotics used in “ischemic stroke” patients provide better outcomes by reducing the incidence of “stroke-associated pneumonia”. Therefore, it should be given to all patients admitted to the hospital with ischemic CVA.
How to cite this: Aslam MJ, Amir M, Sayed TM, Hashmat A, Hussain M, Fatima M.Role of Prophylactic Antibiotics in Preventing Stroke Associated Pneumonia in Patients with Ischemic Stroke. Life and Science. 2024; 5(1): 82-88. doi: http://doi.org/10.37185/LnS.1.1.465
Evaluation of Restless Leg Syndrome in Patients with Non-Dialysis Dependent Chronic Kidney Disease at Fatima Memorial Hospital Lahore: A Cross-sectional Study
Authors: Basit Bilal , Sabir Omer , Shahid Adnan Hussain , Kanwal Andleeb , Abass Qadri Ghulam , Rizvi Nabiha 
Published: January 2024

Objective: To determine the occurrence of restless leg syndrome in individuals with non-dialysis-dependent chronic kidney disease.Study Design: A descriptive cross-sectional study.Place and Duration of Study: The study was carried out at the Outpatient Department (OPD) of Fatima Memorial Hospital, Lahore, Pakistan, from March 2020 to August 2020.Methods: A total of 280 CKD patients not dependent on dialysis were included in the study. The data were collected through interviews containing questions from international restless leg syndrome study group (IRLSSG). Patients were stratified into different stages according to the estimated glomerular filtration rate (eGFR). Restless leg syndrome was diagnosed by analyzing the responses of the interview marked by the examiner. Results: Among 280 patients, 15.7% (n=44) of chronic kidney disease patients were noted to have restless leg syndrome. The syndrome was significantly more frequent in females and was associated with higher CKD stages (Stage V > Stage III and IV). There was no association with either age or BMI.Conclusion: Restless leg syndrome is more common in chronic kidney disease stage V as compared to lower chronic kidney disease stages (III & IV). If untreated, it can affect patients' quality of life and increase the risk of cardiovascular activities.
How to cite this: Basit MB, Sabir O, Shahid AH, Kanwal A, Qadri GA, Rizvi N. Evaluation of Restless Leg Syndrome in Patients with NonDialysis Dependent Chronic Kidney Disease at Fatima Memorial Hospital Lahore: A Cross-sectional study. Life and Science. 2024; 5(1): 95-100.doi: http://doi.org/10.37185/LnS.1.1.516
Diagnostic Accuracy of Serum Cystatin C for Early Detection of Kidney Damage in Patients with Type II Diabetes Mellitus: Bahawalpur, Pakistan
Authors: Sajid Iqra , Saeed Rabia , Goheer Arfa , Asghar Sameen , Saqib Sara , Bashir Fareeha 
Published: January 2024

Objective: To determine the diagnostic accuracy of serum Cystatin C for early detection of kidney damage taking albumin Creatinine Ratio as a reference standard among people of Bahawalpur having diabetes mellitus type 2.Study Design: A cross-sectional study.Place and Duration of Study: The Study was conducted at the Department of Pathology in collaboration with th th Kidney Center, Bahawalpur Victoria Hospital, Bahawalpur, Pakistan from 27 August 2021 to 26 February 2022.Methods: There was a total of 200 patients having diabetes mellitus type 2 with GFR between 60-90ml/min with an age range from 40-60 years were selected. Study participants with a history of steroid intake, hypothyroidism, chronic liver disease, AIDS, and hypertension were not included in the study. For assessment of diagnostic accuracy of Cystatin C to evaluate the renal damages in early stages, microalbuminuria was evaluated. As per the guidelines provided by the respective manufacturers, individuals exhibiting two albumin creatinine ratio (ACR) levels exceeding 30 mg/g were classified as having diabetic nephropathy (DN).Results: The overall sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy of serum Cystatin C for the early detection of kidney damage, with albumin-to-creatinine ratio used as the reference standard among individuals with type 2 diabetes mellitus, were 90.98%, 78.21%, 86.72%, 84.72%, and 86.0%, respectively.Conclusion: The findings of this investigation indicate that serum cystatin C (CysC) exhibits a considerable level of diagnostic accuracy in the early detection of kidney damage among individuals with type 2 diabetes mellitus, utilizing the albumin-to-creatinine ratio as a reference standard.How to cite this: Sajid I, Saeed R, Goheer A, Asghar S, Saqib S, Bashir F. Diagnostic Accuracy of Serum Cystatin C for Early Detection of Kidney Damage in Patients with Type II Diabetes Mellitus: Bahawalpur, Pakistan. Life and Science. 2024; 5(1): 41-47. doi: http://doi.org/10.37185/LnS.1.1.559
Kinds, properties and atelicity
Authors: Chierchia Gennaro 
Published: January 2024

Since at least Vendler 1967, one of the most widely discussed data points, often viewed as the ultimate test for (a)telicity, is the behavior of durative modifiers with respect to different VP types as in John killed mosquitos/*a mosquito for an hour. In the present paper, I explore a new blend of the two most widespread approaches to this issue, namely (i) the view of durative modifiers as universal quantifiers (e.g., Dowty 1979, a.o.) and (ii) their view as aspect sensitive measure adverbials (e.g., Krifka 1998, a.o.). The blend explored here is based on an economy constraint specific to the scope of adverbial quantification (‘do not weaken’ cf. Bassa Vanrell 2017) combined with the identification of the special role that kinds and properties may play as direct bearers of thematic relations in an event-based semantics.
La Importancia de la Nutrición en el Manejo de la Obesidad Infantil: Un Estudio de Revisión
Authors: Ortiz Sánchez Erick Alexander , Hidalgo Morales Kattyta Patricia 
Published: January 2024

Según la OMS (2), la obesidad es “una enfermedad crónica que se caracteriza por el exceso de grasa en el organismo, en un niño se considera que es obeso cuando su peso sobrepasa el 20% de su ideal, mientras que en un adulto es cuando el índice de masa corporal es mayor de 30 kg/m²”. Es por ello que se la considera como una enfermedad crónica, compleja y multifactorial, que generalmente aparece en la infancia y adolescencia que puede tener cierta predisposición genética y ambiental. Por ello, el propósito del presente estudio fue elaborar una revisión bibliográfica referente a la importancia de la nutrición en el manejo de la obesidad infantil, además de cómo prevenirlo a través una buena alimentación desde edades tempranas. Se realizaron búsquedas en sitios como PubMed, MDPI, Dialnet y Google Scholar desde el 12 de julio del 2023 al En la cual, se incluyó, 30 artículos publicados desde el año 2018 y 2023; a partir de los cuales se logró describir los índices y estadísticas de la obesidad infantil, así como, la importancia de una buena alimentación acompañada de ejercicio para mantener una buena salud. Por otro lado de determinó la niñez, es la edad perfecta para crear hábitos alimenticios adecuados, ya que una dieta equilibrada es fundamental para el desarrollo y fortalecimiento del sistema inmunológico; muchos de los problemas de salud que se llegan a presentar en la adultez se encuentran ligadas a una alimentación deficiente durante la infancia.
Improving Phlebotomy Practices Through Small-Volume Blood Tubes: A Survey-Based Study
Authors: ARSLAN Fatma Demet , ÖNCEL VAN Tuğba , ALPŞEN Canan , ÇINAR Sevgi , KÖSEOĞLU Mehmet 
Published: January 2024

Aim: The blood collection for laboratory tests has been frequently performed due to evidence-based medicine. We aimed to conduct a survey on phlebotomy among phlebotomists and patients and to reduce unnecessary blood loss by using small-volume blood collection tubes.
Material and Method: A survey among phlebotomists and patients was conducted to gather their opinions. Phlebotomists received training on the importance of the preanalytical process. The blood volume required for laboratory tests was reduced by 33.3%-50.0% in children and adults, and 63.0%-84.0% in newborns. Following this intervention, we investigated its effects on the blood transfusion ratio in the neonatal and adult intensive care unit (NICU and ICU) and the amount of laboratory medical waste generated.
Results: A majority of phlebotomists (91.8%) reported difficulties in drawing blood from newborns, pediatric, oncology, hematology, and geriatric patients. Additionally, 68.9% of phlebotomists and 57.1% of patients expressed an opinion for reduced blood volume. Despite an increase in the number of laboratory tests (28.4%) and samples (15.7%), we observed a 17.8% reduction in the amount of laboratory medical waste. Although the number of patients in NICU increased statistically significant, the increase in transfusion rates was not significant. Although the number of patients in ICU increased, transfusion rates decreased, but neither was found to be statistically significant.
Conclusion: Post-graduation, phlebotomists should be educated regularly about the preanalytical process. Based on the opinions of both phlebotomists and patients, using small-volume tubes in patients with difficult blood collection may increase their satisfaction. Generally, laboratory medical waste may be reduced.
Evaluation of Publishing Status of Doctoral Theses of Faculty of Medicine Department of Anatomy in Scientific Journals
Authors: ATEŞOĞLU KARABAŞ Sibel , SOLGUN DAĞ Selma 
Published: January 2024

Aim: The aim of this study was to evaluate the publication status of doctoral theses conducted by the anatomy departments of medical faculties in scientific journals.
Material and Method: In this descriptive, cross-sectional study, data were obtained retrospectively from the internet database of the National Thesis Center in the High Education Board (YÖK). A total of 148 doctoral dissertations published between 2009-2019 in the anatomy department of medical faculty were included in the study.
Results: Of the 148 theses, 82 (55.4%) were published in a journal and 66 (44.6%) were unpublished. Of the published theses, 53 (35.8%) were published in SCI/SCI-E, 3 (2%) in TÜBİTAK/Ulakbim TR index and 26 (17.6%) in other international indexes (p=.0001).
Conclusion: It was observed that the publication rate of doctoral dissertations in the anatomy department of medical faculty in Türkiye and the rate of publication as articles in journals in SCI/SCI-E were quite high.
Les efforts de recrutement des employeurs améliorent-ils les appariements sur le marché du travail ?
Authors: Rémy Véronique , Simonnet Véronique 
Published: January 2024

Les efforts de recrutement des employeurs peuvent modifier l’efficacité du processus d’appariement par plusieurs biais dont certains n’ont pas été identifiés. Cet article analyse le lien entre efforts engagés à chaque étape de l’embauche (canaux de recherche, méthodes de sélection, temps imparti, personnel investi, etc.) et qualité des appariements à l’aide d’une typologie des efforts mesurés dans l’enquête Ofer 2016 de la Dares. Les employeurs exigeants et patients sont les plus satisfaits de leurs embauches ainsi que ceux très pressés, très flexibles et limitant leur recherche. Les employeurs les moins satisfaits sont ceux trop pressés pour identifier la meilleure candidature parmi celles recueillies au cours de leur large recherche. Classification JEL : J23, J63, M51.
Du game design au play design : éthos et médiation ludique
Authors: Genvo Sébastien 
Published: January 2024

Dans un premier temps, à partir d’une synthèse de mes travaux antérieurs (Genvo, 2013), je montre comment la notion d’éthos ludique permet de penser et d’analyser ce qui va permettre à un individu de reconnaitre que « ceci est un jeu » (Bateson, 1972, p. 214) et de devenir joueur, mais aussi les écarts sémiotiques qui peuvent se produire lors de l’actualisation de la structure de jeu. Il s’agit de montrer que ce concept, dès lors qu’il est mis en lien avec la notion de contexte pragmatique et de jouabilité, permet d’aller au-delà de l’expérience immédiate pour comprendre la façon dont un objet réussit (ou échoue) à faire sens ludique au sein d’un milieu donné. J’illustre la mise en application de ce cadre théorique par l’étude de deux initiatives de recherche-création menant au développement de jeux expressifs. Le premier, Keys of a gamespace (Expressive Game Lab), a été réalisé en 2011 et le second, Lie in my heart (2019, Expressive Gamestudio), était en cours de développement au moment de la rédaction de l’article. Ces études nous permettent de montrer conjointement comment une interrogation centrée sur l’éthos ludique amène à considérer les enjeux éthiques du processus créatif de game design et engage une réflexion orientée vers le play design (Genvo, 2008).
L’appareil paratextuel vidéoludique comme lieu d’une double médiation : Survival, survivance et intericonicité
Authors: Baychelier Guillaume 
Published: January 2024

Affiches, box art ou encore vidéos constituent un ensemble d’images publicitaires qui accompagnent la parution de la plupart des jeux vidéo. Ce texte s’interroge sur le statut de ces images et sur leur capacité à illustrer, c’est-à-dire à mettre en lumière le contenu de ces jeux — qu’il s’agisse de leur contenu narratif ou ludique. À travers un corpus convoquant l’idée d’horreur (via une iconographie monstrueuse et sanglante), il est montré comment s’opposent plusieurs dynamiques de transposition de l’imaginaire convoqué par ces jeux. Ce texte montre ensuite comment, par l’expression de survivances iconographiques, peut se mettre en place une double médiation ouvrant ces images à un au-delà de la sphère du média jeu vidéo.
Enseignants : le défi de l’attractivité
Authors: Boissinot Alain , Lessard Claude 
Published: January 2024

Recruter en nombre suffisant des enseignants, éviter la démission de ceux qui sont déjà en poste : autant de défis auxquels sont confrontés tous les pays. Les multiples situations analysées dans ce dossier font certes apparaître des différences liées aux contextes sociaux et historiques, mais aussi des constantes qui permettent d’identifier des facteurs décisifs. La reconnaissance du métier, les conditions de travail et d’affectation, la qualité de la formation sont partout évoquées. La comparaison des politiques mises en œuvre pour améliorer le recrutement renvoie à trois enjeux importants : le type d’engagement proposé aux enseignants, l’efficacité des dispositifs de formation, la capacité à dynamiser et à valoriser les évolutions de carrière. Mais par-delà la difficulté à attirer vers le métier d’enseignant, ce qui est en cause est peut-être aussi la capacité des sociétés contemporaines à rendre attractive la mission d’enseignement elle-même.
A Novel Method to Fix Paediatric Mandibular Fracture using 2-0 Polyglactin Suture
Authors: Marimallappa T. R. , Pal Supriyo , Rao Keerthana , Sreelakshmi M. S. 
Published: January 2024

Aim:
This study aimed on a novel cost-effective technique of stabilising the paediatric mandibular fracture using 2-0 resorbable polyglactin 910 suture with minimal trauma to tooth buds and bone and adequate stability.

Background:
Reduction and stabilisation of fractures at the earliest is valuable in the paediatric age groups. This novel technique focuses on stabilisation of the fracture fragments using vicryl, which has an added advantage to conventional plating and wiring.

Technique:
After raising a full-thickness mucoperiosteal flap, the fracture segments are identified and reduced. 1.6mm drill bit is used to make holes through the buccal cortex on either side of the fractured segment in an anteroposterior direction. The patency of holes is checked by passing a long 26-gauge wire. The proximal end of the wire is bent into a loop, a 2-0 resorbable polyglactin suture (vicryl) is passed through this loop and the loop is pressed in place to lock the suture. The wire with the attached suture is pulled through the distal end. Once the suture is secure in place, the 26G wire is cut, and the two ends of sutures are knotted and stabilised. The mucoperiosteal flap is then closed.

Conclusion:
A novel method of stabilisation of paediatric fracture without hampering the tooth and jaw growth, especially in the financially unstable population.

Clinical Significance:
2–0 polyglactin 910 suture is cost-effective, $2 US, compared to resorbable plates costing about $150 US, and easily available. Polyglactin 910 takes 40–60 days for resorption, and half-life tensile strength is 2 weeks; thus, it gives adequate stability and time for callus formation and does not damage the tooth buds or bone by cutting through them.
Management of Complete Persistent Rectal Prolapse in Children
Authors: Gad Mostafa , Dessouky Mostafa Nabil , Abdullateef Khaled Salah , Abdelazim Osama , Fares Ahmed E. , Kaddah Sherif Nabhan , Ragab Moutaz 
Published: January 2024

Abstract

Context:
Rectal prolapse is a relatively common, usually self-limiting illness in children. Peak incidence is between 1 and 3 years. The primary treatment of rectal prolapse is non-operative. Surgical intervention is needed in long-standing intractable cases of rectal prolapse, rectal pain/bleeding/ulceration and prolapse that needs frequent manual or difficult reduction.

Aim of the Study:
The aim of this study was to compare the efficacy and outcome of laparoscopic ventral mesh rectopexy versus laparoscopic suture rectopexy in the management of persistent rectal prolapse in children not responding to conservative management and/or recurrent after sclerotherapy or anal encirclement.

Methods:
Twenty-four cases were randomised into two groups at the ratio of 1:1, Group 1 patients were managed by laparoscopic ventral mesh rectopexy, whereas Group 2 cases were managed by laparoscopic suture rectopexy. Patients with primary surgical conditions such as anorectal malformations, Hirschsprung’s disease, rectal polyps or masses and Ectopia Vesicae were excluded from the study. Inclusion criteria were complete rectal prolapse cases with failed medical treatment for at least 6 months and/or recurrent after injection sclerotherapy or anal encirclement.

Results:
In the mesh rectopexy group, one case had recurrence in the form of partial prolapse 3 weeks postoperatively which improved 2 months postoperatively with conservative management, one case had bleeding per rectum 2 months postoperatively, stool analysis was done revealing parasitic infestation which was treated medically. In the suture rectopexy group, one case had one attack of bleeding per rectum on the 2nd day postoperatively which resolved spontaneously and one case was readmitted on the 5th day postoperatively for non-bilious vomiting which improved by medical treatment. No recurrent cases of complete rectal prolapse were reported in both groups.

Conclusion:
Laparoscopic rectopexy can be an effective modality for the treatment of refractory complete rectal prolapse in children. It is effective, safe and easy. Although the current study has shown that laparoscopic suture rectopexy and mesh rectopexy have nearly the same results, a larger number of patients are needed to compare more deeply between the two groups.