bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of trade on global aquatic food consumption patterns
Authors: Zhao Kangshun , Gaines Steven D. , García Molinos Jorge , Zhang Min , Xu Jun 
Published: February 2024

AbstractGlobalization of fishery products is playing a significant role in shaping the harvesting and use of aquatic foods, but a vigorous debate has focused on whether the trade is a driver of the inequitable distribution of aquatic foods. Here, we develop species-level mass balance and trophic level identification datasets for 174 countries and territories to analyze global aquatic food consumption patterns, trade characteristics, and impacts from 1976 to 2019. We find that per capita consumption of aquatic foods has increased significantly at the global scale, but the human aquatic food trophic level (HATL), i.e., the average trophic level of aquatic food items in the human diet, is declining (from 3.42 to 3.18) because of the considerable increase in low-trophic level aquaculture species output relative to that of capture fisheries since 1976. Moreover, our study finds that trade has contributed to increasing the availability and trophic level of aquatic foods in >60% of the world’s countries. Trade has also reduced geographic differences in the HATL among countries over recent decades. We suggest that there are important opportunities to widen the current focus on productivity gains and economic outputs to a more equitable global distribution of aquatic foods.
Reconstitution of early paclitaxel biosynthetic network
Authors: Liu Jack Chun-Ting , De La Peña Ricardo , Tocol Christian , Sattely Elizabeth S. 
Published: February 2024

AbstractPaclitaxel is an anticancer therapeutic produced by the yew tree. Over the last two decades, a significant bottleneck in the reconstitution of early paclitaxel biosynthesis has been the propensity of heterologously expressed pathway cytochromes P450, including taxadiene 5α-hydroxylase (T5αH), to form multiple products. Here, we structurally characterize four new products of T5αH, many of which appear to be over-oxidation of the primary mono-oxidized products. By tuning the promoter strength for T5αH expression in Nicotiana plants, we observe decreased levels of these proposed byproducts with a concomitant increase in the accumulation of taxadien-5α-ol, the paclitaxel precursor, by three-fold. This enables the reconstitution of a six step biosynthetic pathway, which we further show may function as a metabolic network. Our result demonstrates that six previously characterized Taxus genes can coordinatively produce key paclitaxel intermediates and serves as a crucial platform for the discovery of the remaining biosynthetic genes.
Chp1 is a dedicated chaperone at the ribosome that safeguards eEF1A biogenesis
Authors: Minoia Melania , Quintana-Cordero Jany , Jetzinger Katharina , Kotan Ilgin Eser , Turnbull Kathryn Jane , Ciccarelli Michela , Masser Anna E. , Liebers Dorina , Gouarin Eloïse , Czech Marius , Hauryliuk Vasili , Bukau Bernd , Kramer Günter , Andréasson Claes 
Published: February 2024

AbstractCotranslational protein folding depends on general chaperones that engage highly diverse nascent chains at the ribosomes. Here we discover a dedicated ribosome-associated chaperone, Chp1, that rewires the cotranslational folding machinery to assist in the challenging biogenesis of abundantly expressed eukaryotic translation elongation factor 1A (eEF1A). Our results indicate that during eEF1A synthesis, Chp1 is recruited to the ribosome with the help of the nascent polypeptide-associated complex (NAC), where it safeguards eEF1A biogenesis. Aberrant eEF1A production in the absence of Chp1 triggers instant proteolysis, widespread protein aggregation, activation of Hsf1 stress transcription and compromises cellular fitness. The expression of pathogenic eEF1A2 variants linked to epileptic-dyskinetic encephalopathy is protected by Chp1. Thus, eEF1A is a difficult-to-fold protein that necessitates a biogenesis pathway starting with dedicated folding factor Chp1 at the ribosome to protect the eukaryotic cell from proteostasis collapse.
Tunable templating of photonic microparticles via liquid crystal order-guided adsorption of amphiphilic polymers in emulsions
Authors: Ma Xu , Han Yucen , Zhang Yan-Song , Geng Yong , Majumdar Apala , Lagerwall Jan P. F. 
Published: February 2024

AbstractMultiple emulsions are usually stabilized by amphiphilic molecules that combine the chemical characteristics of the different phases in contact. When one phase is a liquid crystal (LC), the choice of stabilizer also determines its configuration, but conventional wisdom assumes that the orientational order of the LC has no impact on the stabilizer. Here we show that, for the case of amphiphilic polymer stabilizers, this impact can be considerable. The mode of interaction between stabilizer and LC changes if the latter is heated close to its isotropic state, initiating a feedback loop that reverberates on the LC in form of a complete structural rearrangement. We utilize this phenomenon to dynamically tune the configuration of cholesteric LC shells from one with radial helix and spherically symmetric Bragg diffraction to a focal conic domain configuration with highly complex optics. Moreover, we template photonic microparticles from the LC shells by photopolymerizing them into solids, retaining any selected LC-derived structure. Our study places LC emulsions in a new light, calling for a reevaluation of the behavior of stabilizer molecules in contact with long-range ordered phases, while also enabling highly interesting photonic elements with application opportunities across vast fields.
Global and regional ocean mass budget closure since 2003
Authors: Ludwigsen Carsten Bjerre , Andersen Ole Baltazar , Marzeion Ben , Malles Jan-Hendrik , Müller Schmied Hannes , Döll Petra , Watson Christopher , King Matt A. 
Published: February 2024

AbstractIn recent sea level studies, discrepancies have arisen in ocean mass observations obtained from the Gravity Recovery and Climate Experiment and its successor, GRACE Follow-On, with GRACE estimates consistently appearing lower than density-corrected ocean volume observations since 2015. These disparities have raised concerns about potential systematic biases in sea-level observations, with significant implications for our understanding of this essential climate variable. Here, we reconstruct the global and regional ocean mass change through models of ice and water mass changes on land and find that it closely aligns with both GRACE and density-corrected ocean volume observations after implementing recent adjustments to the wet troposphere correction and halosteric sea level. While natural variability in terrestrial water storage is important on interannual timescales, we find that the net increase in ocean mass over 20 years can be almost entirely attributed to ice wastage and human management of water resources.
Global freshwater fish invasion linked to the presence of closely related species
Authors: Xu Meng , Li Shao-peng , Liu Chunlong , Tedesco Pablo A. , Dick Jaimie T. A. , Fang Miao , Wei Hui , Yu Fandong , Shu Lu , Wang Xuejie , Gu Dangen , Mu Xidong 
Published: February 2024

AbstractIn the Anthropocene, non-native freshwater fish introductions and translocations have occurred extensively worldwide. However, their global distribution patterns and the factors influencing their establishment remain poorly understood. We analyze a comprehensive database of 14953 freshwater fish species across 3119 river basins and identify global hotspots for exotic and translocated non-native fishes. We show that both types of non-native fishes are more likely to occur when closely related to native fishes. This finding is consistent across measures of phylogenetic relatedness, biogeographical realms, and highly invaded countries, even after accounting for the influence of native diversity. This contradicts Darwin’s naturalization hypothesis, suggesting that the presence of close relatives more often signifies suitable habitats than intensified competition, predicting the establishment of non-native fish species. Our study provides a comprehensive assessment of global non-native freshwater fish patterns and their phylogenetic correlates, laying the groundwork for understanding and predicting future fish invasions in freshwater ecosystems.
Cell softness renders cytotoxic T lymphocytes and T leukemic cells resistant to perforin-mediated killing
Authors: Zhou Yabo , Wang Dianheng , Zhou Li , Zhou Nannan , Wang Zhenfeng , Chen Jie , Pang Ruiyang , Fu Haixia , Huang Qiusha , Dong Fang , Cheng Hui , Zhang Huafeng , Tang Ke , Ma Jingwei , Lv Jiadi , Cheng Tao , Fiskesund Roland , Zhang Xiaohui , Huang Bo 
Published: February 2024

AbstractMechanical force contributes to perforin pore formation at immune synapses, thus facilitating the cytotoxic T lymphocytes (CTL)-mediated killing of tumor cells in a unidirectional fashion. How such mechanical cues affect CTL evasion of perforin-mediated autolysis remains unclear. Here we show that activated CTLs use their softness to evade perforin-mediated autolysis, which, however, is shared by T leukemic cells to evade CTL killing. Downregulation of filamin A is identified to induce softness via ZAP70-mediated YAP Y357 phosphorylation and activation. Despite the requirements of YAP in both cell types for softness induction, CTLs are more resistant to YAP inhibitors than malignant T cells, potentially due to the higher expression of the drug-resistant transporter, MDR1, in CTLs. As a result, moderate inhibition of YAP stiffens malignant T cells but spares CTLs, thus allowing CTLs to cytolyze malignant cells without autolysis. Our findings thus hint a mechanical force-based immunotherapeutic strategy against T cell leukemia.
Highly permeable and shelf-stable aquaporin biomimetic membrane based on an anodic aluminum oxide substrate
Authors: Fuwad Ahmed , Ryu Hyunil , Han Eui Don , Lee Jun-Hee , Malmstadt Noah , Kim Young-Rok , Seo Young Ho , Kim Sun Min , Jeon Tae-Joon 
Published: February 2024

AbstractAquaporin (AQP) biomimetic membranes are a coming-of-age technology for water purification. Although several studies have reported aquaporin biomimetic membrane fabrication to date, these membranes show low water flux mainly due to the low porosity and inherently dense structure of the polymeric substrate materials. Herein, we report a ceramic-based aquaporin biomimetic membrane based on anodic aluminum oxide (AAO) as a substrate, which has a uniform porous structure with a high aspect ratio and pore density compared to conventional polymer substrates and exhibits a high water flux of 27.6 ± 3.6 LMH (L m−2 h−1) and superior membrane selectivity of 0.11 g L−1. Briefly, the AAO substrate was functionalized with amino-silane followed by polydopamine coating, then the AQP vesicles were immobilized on the functionalized AAO substrate surface using an electrokinetic method, and the water rejection performance of the membrane was analyzed in a forward osmosis system. Furthermore, a simple cryodesiccation method is introduced to improve the storage stability and easy transportation of aquaporin membranes, which does not require special environmental conditions to transport or store them.
3D hierarchical aquaporin-like nanoporous graphene membrane with engineered tripartite nanochannels for efficient oil/water separation
Authors: Hegab Hanaa M. , Elmekawy Ahmed , Aubry Cyril , Kallem Parashuram , Wadi Vijay S. , Banat Fawzi , Hasan Shadi W. 
Published: February 2024

AbstractThe aqueous system is being polluted by the untreated direct discharge of industrial oily wastewater into the ecosystem. Due to its low cost, energy economy, and sustainability, the advanced membrane filtration method is regarded as one of the best methods for treating oily wastewater. Its exceptional atomic thickness and superior amphiphilic properties of graphene oxide (GO) nanosheet make it one of the finest 2D constituents for creating membranes with high permeability. Nevertheless, the interlayer d-spacing of multi-stacked GO membranes is crucial since it is responsible for the permeability/selectivity trade-off. To efficiently separate oil-in-water emulsion using an in-situ polymerization technique, we developed an aquaporin-like 3D hierarchical multi-functionalized nanoporous graphene (NPG) membrane with tripartite nanochannels. The extraordinary, prepared membrane displayed both ultra-water-permeability of 2490 L m−2 h−1.bar along with superior selectivity. Consequently, the permeance of the aquaporin-like 3D hierarchical multi-functionalized NPG membrane achieves a higher flux than the GO membrane, while the oil rejection reaches ~97%.
Validation of portable in-clinic video-based gait analysis for prosthesis users
Authors: Cimorelli Anthony , Patel Ankit , Karakostas Tasos , Cotton R. James 
Published: February 2024

AbstractDespite the common focus of gait in rehabilitation, there are few tools that allow quantitatively characterizing gait in the clinic. We recently described an algorithm, trained on a large dataset from our clinical gait analysis laboratory, which produces accurate cycle-by-cycle estimates of spatiotemporal gait parameters including step timing and walking velocity. Here, we demonstrate this system generalizes well to clinical care with a validation study on prosthetic users seen in therapy and outpatient clinics. Specifically, estimated walking velocity was similar to annotated 10-m walking velocities, and cadence and foot contact times closely mirrored our wearable sensor measurements. Additionally, we found that a 2D keypoint detector pretrained on largely able-bodied individuals struggles to localize prosthetic joints, particularly for those individuals with more proximal or bilateral amputations, but after training a prosthetic-specific joint detector video-based gait analysis also works on these individuals. Further work is required to validate the other outputs from our algorithm including sagittal plane joint angles and step length. Code for the gait transformer and the trained weights are available at https://github.com/peabody124/GaitTransformer.
Genetic variants associated with osteosarcoma risk: a systematic review and meta-analysis
Authors: Hassanain Omneya , Alaa Mahmoud , Khalifa Mohamed K. , Kamal Nehal , Albagoury Aseel , El Ghoneimy Ahmed M. 
Published: February 2024

AbstractOsteosarcoma (OS) is the most common type of primary bone malignancy. Common genetic variants including single nucleotide polymorphisms (SNPs) have been associated with osteosarcoma risk, however, the results of published studies are inconsistent. The aim of this study was to systematically review genetic association studies to identify SNPs associated with osteosarcoma risk and the effect of race on these associations. We searched the Medline, Embase, Scopus from inception to the end of 2019. Seventy-five articles were eligible for inclusion. These studies investigated the association of 190 SNPs across 79 genes with osteosarcoma, 18 SNPs were associated with the risk of osteosarcoma in the main analysis or in subgroup analysis. Subgroup analysis displayed conflicting effects between Asians and Caucasians. Our review comprehensively summarized the results of published studies investigating the association of genetic variants with osteosarcoma susceptibility, however, their potential value should be confirmed in larger cohorts in different ethnicities.
Ex-vivo validation of spatial gain sonography for the quantification of echo intensity in fascicle-aligned ultrasound images in ten anatomical muscles in Bos taurus
Authors: Rosahl Sophie C. , Rauschendorfer Philipp , Arndt Lukas , Voigtmann Thomas , Mittag Uwe , Rittweger Jörn 
Published: February 2024

AbstractThis study aimed to validate the concept of spatial gain sonography for quantifying texture-related echo intensity in B-mode ultrasound of skeletal muscle. Fifty-one bovine muscles were scanned postmortem using B-mode ultrasonography at varying fascicle probe angles (FPA). The relationship between mean gray values (MGV) and FPA was fitted with a sinusoidal and a linear function, the slope of which was defined as tilt echo gain (TEG). Macroscopic muscle cross sections were optically analyzed for intramuscular connective tissue (IMCT) content which was plotted against MGV at 0° FPA (MGV_00). MGV peaked at FPA 0°. Sine fits were superior to linear fits (adjusted r2-values 0.647 vs. 0.613), especially for larger FPAs. In mixed models, the pennation angle was related to TEG (P < 0.001) and MGV_00 (P = 0.035). Age was relevant for MGV_00 (P < 0.001), but not TEG (P > 0.10). The correlation between the IMCT percentage and MGV_00 was significant but weak (P = 0.026; adjusted r2 = 0.103). The relationship between fascicle probe angle and echo intensity in B-mode ultrasound can be modeled more accurately with a sinusoidal but more practically for clinical use with a linear fit. The peak mean gray value MGV_00 can be used to compare echo intensity across muscles without the bias of pennation angle.
Diabetic peripheral neuropathy among adult type 2 diabetes patients in Adama, Ethiopia: health facility-based study
Authors: Mekuria Negussie Yohannes , Tilahun Bekele Nardos 
Published: February 2024

AbstractDiabetic peripheral neuropathy is the most prominent microvascular complication of diabetes mellitus and the leading cause of ulceration, amputation, and extended hospitalization. Evidence regarding the magnitude and factors associated with diabetic peripheral neuropathy is not well documented in Ethiopia, particularly in the study area. A facility-based cross-sectional study was conducted among 293 adult type 2 diabetic patients who were on treatment and follow-up from May to June 31, 2023. To select participants in the study, a systematic random sampling method was utilized. Data were collected using semi-structured questionnaires and medical record reviews. The Michigan Neuropathy Screening Instrument (MNSI) was employed to assess diabetic peripheral neuropathy. To model the association between diabetic peripheral neuropathy and independent variables, binary logistic regression model was used. An adjusted odds ratio with a 95% confidence interval was used to estimate the association and statistical significance was proclaimed at a p-value < 0.05. The magnitude of diabetic peripheral neuropathy was 14.3% (95% CI 10.4–18.0). It was 13.4% (95% CI 8.4–19.1) among males and 15.4% (95% CI 10.1–22.2) among females. Age above 60 years (AOR = 5.06, 95% CI 1.60–15.96), being rural resident (AOR = 2.41; 95% CI 1.15–5.06), duration of diabetes above 5 years (AOR = 2.48, 95% CI 1.16–5.27) and having comorbid hypertension (AOR = 2.56, 95% CI 1.24–5.28) were independently associated with diabetic peripheral neuropathy. One in seven adult type 2 diabetes patients in the study area had diabetic peripheral neuropathy. Factors such as age, place of residence, duration of diabetes, and comorbid hypertension showed positive associations with diabetic peripheral neuropathy. Thus, it is imperative to give special consideration to diabetic patients who are elderly, living in rural areas, experiencing a prolonged duration of diabetes, or dealing with comorbid hypertension.
Elevated platelet count is associated with decreased mortality from hemorrhagic stroke in hospital: a multi-center retrospective cohort study
Authors: Huang Zhenhua , Liu Chenglin , Wu Zhanxing , Xiao Xiaoyong , Chen Zhongqin , Huang Qun , Liu Dehong , Deng Zhe 
Published: February 2024

AbstractThis study aimed to investigate the relationship between platelet count (PC) and mortality in patients with hemorrhagic stroke (HS). The research reviewed data from 10,466 patients hospitalized in 208 hospitals in the United States from January 1, 2014, to December 31, 2015. Of these, 3262 HS patients were included in the primary analysis for those admitted to the intensive care unit (ICU). The average age of these patients was 67.05 years, with 52.79% being male. The median PC was (221.67 ± 73.78) × 109/L. Multivariate logistic regression analysis revealed that PC was a protective factor for mortality in HS patients (OR = 0.98, 95% CI 0.97–1.00, P < 0.05). Additionally, a non-linear association between PC and mortality in HS patients was found using a generalized additive model (GAM) and smooth curve fitting (penalty spline method). For the first time, a recursive algorithm identified the inflection point of platelet count as 194 × 109/L. On the left side of the inflection point, for every increase of 10 units in platelet count, the mortality rate of HS patients decreases by 10%. The study demonstrates a non-linear relationship between PC and the risk of mortality in HS patients. A platelet counts higher than the inflection point (194 × 109/L) may be a significant intervention to reduce mortality in HS patients.
Asymmetric imaging through engineered Janus particle obscurants using a Monte Carlo approach for highly asymmetric scattering media
Authors: da Mota Achiles F. , Sadafi Mohammad Mojtaba , Mosallaei Hossein 
Published: February 2024

AbstractThe advancement of imaging systems has significantly ameliorated various technologies, including Intelligence Surveillance Reconnaissance Systems and Guidance Systems, by enhancing target detection, recognition, identification, positioning, and tracking capabilities. These systems can be countered by deploying obscurants like smoke, dust, or fog to hinder visibility and communication. However, these counter-systems affect the visibility of both sides of the cloud. In this sense, this manuscript introduces a new concept of a smoke cloud composed of engineered Janus particles to conceal the target image on one side while providing clear vision from the other. The proposed method exploits the unique scattering properties of Janus particles, which selectively interact with photons from different directions to open up the possibility of asymmetric imaging. This approach employs a model that combines a genetic algorithm with Discrete Dipole Approximation to optimize the Janus particles' geometrical parameters for the desired scattering properties. Moreover, we propose a Monte Carlo-based approach to calculate the image formed as photons pass through the cloud, considering highly asymmetric particles, such as Janus particles. The effectiveness of the cloud in disguising a target is evaluated by calculating the Probability of Detection (PD) and the Probability of Identification (PID) based on the constructed image. The optimized Janus particles can produce a cloud where it is possible to identify a target more than 50% of the time from one side (PID > 50%) while the target is not detected more than 50% of the time from the other side (PD < 50%). The results demonstrate that the Janus particle-engineered smoke enables asymmetric imaging with simultaneous concealment from one side and clear visualization from the other. This research opens intriguing possibilities for modern obscurant design and imaging systems through highly asymmetric and inhomogeneous particles besides target detection and identification capabilities in challenging environments.