bluuebunny/crossref_metadata_2025_split huggingface dataset:
Astragaloside IV improves slow transit constipation by regulating gut microbiota and enterochromaffin cells
Authors: Wan Xingyang , Zhou Qian , Chen Huaxian , Li Zhen , Mo Mianling , Liu Zhimin , Zhang Heng , He Zhuojie , Xiao Guozhong , Zheng Yihui , Lin Hongcheng , Ren Donglin 
Published: November 2023

Purpose: Slow transit constipation (STC) is a common gastrointestinal disorder characterized by altered gut microbiota and reduced number of enterochromaffin cells (ECs). Astragaloside IV (AS-IV), a low drug permeability saponin, has showed beneficial effects on patients with STC. However, the specific mechanism by which AS-IV regulates STC remains unclear. In this study, we aimed to investigate the effect of AS-IV on STC and its associated mechanisms involving gut microbiota.Methods: The effect of AS-IV on STC was evaluated on STC mice induced with loperamide. We measured defecation frequency, intestinal mobility, ECs loss, and colonic lesions in STC mice treated with AS-IV. We also analyzed the changes in gut microbiota and metabolites after AS-IV treatment. Moreover, we investigated the relationship between specific gut microbes and altered fecal metabolites, such as 3-bromotyrosine (3-BrY). We also conducted in vitro experiments to investigate the effect of 3-BrY on caspase-dependent apoptosis of ECs and the activation of the p38 MAPK and ERK signaling pathways induced by loperamide.Results: AS-IV treatment promoted defecation, improved intestinal mobility, suppressed ECs loss, and alleviated colonic lesions in STC mice. AS-IV treatment also affected gut microbiota and metabolites, with a significant correlation between specific gut microbes and altered fecal metabolites such as 3-BrY. Furthermore, 3-BrY may potentially reduce caspase-dependent apoptosis of ECs and protect cell survival by inhibiting the activation of the p38 MAPK and ERK signaling pathways induced by loperamide.Conclusion: Our findings suggest that changes in gut microbiota and ECs mediated the therapeutic effect of STC by AS-IV. These results provide a basis for the use of AS-IV as a prebiotic agent for treating STC. The specific mechanism by which AS-IV regulates gut microbiota and ECs warrants further investigation.
Total flavonoids extracted from Penthorum chinense Pursh mitigates CCl4-induced hepatic fibrosis in rats via inactivation of TLR4-MyD88-mediated NF-κB pathways and regulation of liver metabolism
Authors: Wang Sujuan , Li Wenqing , Liu Wenxiu , Yu Lei , Peng Fu , Qin Junyuan , Pu Lin , Tang Yunli , Xie Xiaofang , Peng Cheng 
Published: November 2023

Background:Penthorum chinense Pursh (PCP) is widely utilized in China to treat a variety of liver diseases. It has been shown that flavonoids inhibit inflammation and have the potential to attenuate tissue damage and fibrosis. However, the mechanisms underlying how total flavonoids isolated from PCP (TFPCP) exert their anti-fibrotic effects remain unclear.Methods: The chemical composition of TFPCP was determined using UHPLC–Q-Orbitrap HRMS. Subsequently, rats were randomly assigned to a control group (Control), a carbon tetrachloride (CCl4)-induced hepatic fibrosis model group (Model), a positive control group [0.2 mg/(kg∙day)] of Colchicine), and three TFPCP treatment groups [50, 100, and 150 mg/(kg∙day)]. All substances were administered by gavage and treatments lasted for 9 weeks. Simultaneously, rats were intraperitoneally injected with 10%–20% CCl4 for 9 weeks to induce liver fibrosis. At the end of the experiment, the liver ultrasound, liver histomorphological, biochemical indicators, and inflammatory cytokine levels were tested respectively. The underlying mechanisms were assessed using Western blot, immunohistochemistry, immunofluorescence, RT-qPCR, and metabolomics.Results: Fourteen flavonoids were identified in TFPCP. Compared with control animals, CCl4-treated rats demonstrated obvious liver injury and fibrosis, manifested as increases in gray values, distal diameter of portal vein (DDPV) and a decrease in blood flow velocity (VPV) in the ultrasound analysis; increased biochemical index values (serum levels of ALT, AST, TBIL, and ALP); marked increases in the contents of fibrotic markers (PC III, COL4, LN, HA) and inflammatory factors (serum TNF-α, IL-6, and IL-1β); and significant pathological changes. However, compared with the Model group, the ultrasound parameters were significantly improved and the serum levels of inflammatory cytokines were reduced in the TFPCP group. In contrast, the expression of TGF-β1, TLR4, and MyD88, as well as the p-P65/P65 and p-IκBα/IκBα ratios, were considerably reduced following TFPCP treatment. In addition, we identified 32 metabolites exhibiting differential abundance in the Model group. Interestingly, TFPCP treatment resulted in the restoration of the levels of 20 of these metabolites.Conclusion: Our findings indicated that TFPCP can ameliorate hepatic fibrosis by improving liver function and morphology via the inactivation of the TLR4/MyD88-mediated NF-κB pathway and the regulation of liver metabolism.
Pharmacokinetics, pharmacodynamics, and safety of ciprofol emulsion in Chinese subjects with normal or impaired renal function
Authors: Tao Jun , Liu Shuaibing , Zhao Ying Ying , Qi Lei , Yan Pangke , Wu Nan , Liu Xiao , Liu Dongwei , Tian Xin 
Published: November 2023

Background: Ciprofol, a novel sedative–hypnotic drug, has been approved for its use in inducing and maintaining general anesthesia, as well as for providing sedation.Methods: In this phase I, single-center, parallel, controlled, open-label clinical trial, our objective was to analyze the pharmacokinetics (PK), pharmacodynamics (PD), and safety of ciprofol emulsion in 24 participants with mild and moderate renal impairment (n = 8 per group) and matched healthy participants (n = 8). An initial loading infusion of ciprofol was administered at 0.4 mg/kg for 1 min, followed by a maintenance infusion at a rate of 0.4 mg/kg/h for 30 min. We collected plasma and urine samples from the participants to assess the PK of ciprofol and its metabolite M4. The evaluation of PD involved using a modified observer’s alertness/sedation scale (MOAA/S) in combination with bispectral index (BIS) monitoring. Safety assessments were conducted throughout the trial process.Results: The plasma concentration–time curve of ciprofol in participants with renal impairment was similar to that in participants with normal kidney function. The area under the curve (AUC) and maximum concentration (Cmax) of total and unbound ciprofol in plasma for participants with renal impairment were only slightly higher (0.7–1.2-fold) than those in participants with normal renal function. In contrast, for the metabolite M4, AUC values were 1.3- and 2.1-fold greater in participants with mild and moderate renal impairment, respectively, than in healthy controls. However, renal impairment had no significant impact on the PD parameters. The study found that ciprofol was well-tolerated, with all adverse events (AEs) reported being mild or moderate in severity.Conclusion: Based on these findings, we can conclude that no dosage adjustment of ciprofol is necessary for patients with mild–moderate renal impairment who receive the injection.Clinical Trial Registration:https://clinicaltrials.gov, identifier NCT04142970.
Gut microbiome and metabolome in aneurysm rat with hypertension after ginsenoside Rb1 treatment
Authors: Zeng Zhaobin , Wang Haibin , Yi Renhui , Lou Jianyun , Wen Shuting , Hu Zheng 
Published: November 2023

Introduction: Hypertension is a well-known risk factor for aneurysms, as high blood pressure can worsen the development and rupture of aneurysms. Ginsenoside, derived from ginseng and widely used in traditional herbal medicine, is believed to have antihypertensive properties. Recent research has also shown a connection between gut microbiota and various diseases, including hypertension. However, the relationship between ginsenosides, gut microbiota, blood pressure, and intracranial aneurysms needs further exploration.Methods: In this study, a rat model was used to investigate the effects of ginsenosides on both blood pressure and intracranial arteries. Comparative analysis was conducted, and 16S rRNA sequencing was employed to identify marker genera within the gut microbiota. Metabolites were also analyzed to uncover potential mediators of blood pressure regulation.Results and Discussion: The results of this study revealed that ginsenosides, particularly ginsenoside Rb1, demonstrated positive effects in reducing both blood pressure and the development of intracranial aneurysms in rats. Furthermore, the analysis of gut microbiota showed that certain genera, including Clostridium, Roseburia, Ruminococcus, and Treponema, were significantly influenced by ginsenoside treatment. Several metabolites, such as behenic acid, N-Acetylserotonin, Prostaglandin F2a, and Vitamin D2, were also detected, all of which play a role in regulating blood pressure. These findings provide valuable insights into the potential benefits of ginsenosides in hypertension and atheroma development. Furthermore, they suggest a possible link between ginsenosides, gut microbiota, and blood pressure regulation. Further research is needed to fully understand the mechanisms underlying these effects and to determine the clinical implications for treating hypertension and reducing the risk of aneurysm development.
Predictive modeling of Alfvén eigenmode stability in inductive scenarios in JT-60SA
Authors: Coelho R. , Vincenzi P. , Vallar M. , Rodrigues P. , Tholerus E. , Särkimäki K. , Garcia J. , Borba D. , Nabais F. , Calado R. , Ferreira J. , Figueiredo A. 
Published: November 2023

The JT-60SA device offers unique conditions before ITER for the study of the interaction of energetic particles with plasma waves. With similar dimensions to JET, e.g., a major radius but with a slightly more elongated plasma volume, JT-60SA is used as a high-power device where additional heating power (including 10 MW of the 500 keV Neutral Beam Injection) of up to 41 MW and the potential for high non-inductive plasma current operation pave the path for numerous challenges in physics on MHD stability, in particular, when considering the effects of energetic particles. Several operational scenarios with ITER and DEMO-relevant plasma regimes, in terms of non-dimensional plasma parameters, are anticipated. In this work, the stability of Alfvén eigenmodes (AEs) in variants of two of the most relevant operational scenarios with single null is analyzed: a full Ip inductive scenario at high density (1.1 × 1020 m−3 on-axis electron density) and 5.48MA/2.05T toroidal plasma current and magnetic field, and an advanced (hybrid) scenario with an ion energy transport barrier (ITB) and 3.5MA/2.28T toroidal plasma current and magnetic field. The workflow included the CRONOS code to establish the scenario, the ASCOT code to calculate the slowing-down energetic particle distributions for a positive/negative ion source-based neutral beam, and the MISHKA/CASTOR-K suite to calculate the MHD spectra of AEs and the associated drive/damping contributions from the NBI energetic ions, as well as the thermal ion landau damping. The systematic analysis, over a large Fourier space of the toroidal mode number/mode frequency, provides evidence that although a significant fraction of supra-Alfvénic particles stemming from the negative ion source-based neutral beam (500 keV) can, in some cases, drive to AEs in both scenarios, it is not enough to overcome the thermal ion landau damping. In addition, the advanced scenario with ITB is shown to be stable against AEs localized in the vicinity of the barrier as well, offering good prospects of sustainability of the plasma performance and of ITB. Finally, some sensitivity scan results are shown on the influence of fast ion density and q-profile on the AE mode spectra and stability.
Global tendencies and frontier topics in hemodynamics research of intracranial aneurysms: a bibliometric analysis from 1999 to 2022
Authors: Chen Bo , Huang Siting , Zhang Liyang , Yang Liting , Liu Yuanyuan , Li Chuntao 
Published: November 2023

Background: Hemodynamics plays a crucial role in the initiation, enlargement, and rupture of intracranial aneurysms (IAs). This bibliometric analysis aimed to map the knowledge network of IA hemodynamic research.Methods: Studies on hemodynamics in IAs published from 1999 to 2022 were retrieved from the Web of Science Core Collection (WoSCC). The contributions of countries, institutions, authors, and journals were identified using VOSviewer, Scimago Graphica, and Microsoft Excel. Tendencies, frontier topics, and knowledge networks were analyzed and visualized using VOSviewer and CiteSpace.Results: We identified 2,319 publications on hemodynamics in IAs. The annual number of publications exhibited an overall increasing trend. Among these, the United States, Japan, and China were the three major contributing countries. Capital Medical University, State University of New York (SUNY) Buffalo University, and George Mason University were the three most productive institutions. Meng H ranked first among authors regarding the number of articles and citations, while Cebral JR was first among co-cited authors. The American Journal of Neuroradiology was the top journal in terms of the number of publications, citations, and co-citations. In addition, the research topics can be divided into three clusters: hemodynamics itself, the relationship of hemodynamics with IA rupture, and the relationship of hemodynamics with IA treatment. The frontier directions included flow diverters, complications, morphology, prediction, recanalization, and four-dimensional flow magnetic resonance imaging (4D flow MRI).Conclusion: This study drew a knowledge map of the top countries, institutions, authors, publications, and journals on IA hemodynamics over the past 2 decades. The current and future hotspots of IA hemodynamics mainly include hemodynamics itself (4D flow MRI), its relationship with IA rupture (morphology and prediction), and its relationship with IA treatment (flow diverters, complications, and recanalization).
Assistive diagnostic technology for congenital heart disease based on fusion features and deep learning
Authors: Wang Yuanlin , Yang Xuankai , Qian Xiaozhao , Wang Weilian , Guo Tao 
Published: November 2023

Introduction:Congenital heart disease (CHD) is a cardiovascular disorder caused by structural defects in the heart. Early screening holds significant importance for the effective treatment of this condition. Heart sound analysis is commonly employed to assist in the diagnosis of CHD. However, there is currently a lack of an efficient automated model for heart sound classification, which could potentially replace the manual process of auscultation.Methods:This study introduces an innovative and efficient screening and classification model, combining a locally concatenated fusion approach with a convolutional neural network based on coordinate attention (LCACNN). In this model, Mel-frequency spectral coefficients (MFSC) and envelope features are locally fused and employed as input to the LCACNN network. This model automatically analyzes feature map energy information, eliminating the need for denoising processes.Discussion:The proposed classification model in this study demonstrates a robust capability for identifying congenital heart disease, potentially substituting manual auscultation to facilitate the detection of patients in remote areas.Results:This study introduces an innovative and efficient screening and classification model, combining a locally concatenated fusion approach with a convolutional neural network based on coordinate attention (LCACNN). In this model, Mel-frequency spectral coefficients (MFSC) and envelope features are locally fused and employed as input to the LCACNN network. This model automatically analyzes feature map energy information, eliminating the need for denoising processes. To assess the performance of the classification model, comparative ablation experiments were conducted, achieving classification accuracies of 91.78% and 94.79% on the PhysioNet and HS databases, respectively. These results significantly outperformed alternative classification models.
Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatments
Authors: Zhong Chao , Liu Yu , Li Zhao , Wang Xiaoguang , Jiang Chunji , Zhao Xinhua , Kang Shuli , Liu Xibo , Zhao Shuli , Wang Jing , Zhang He , Huang Yuning , Yu Haiqiu , Xue Renfeng 
Published: November 2023

IntroductionThe TGA transcription factors, plays a crucial role in regulating gene expression. In cultivated peanut (Arachis hypogaea), which faces abiotic stress challenges, understanding the role of TGAs is important.MethodsIn this study, we conducted a comprehensive in analysis of the TGA gene family in peanut to elucidate their regulatory mechanisms and expression patterns under abiotic stress and hormone treatments. Furthermore, functional studies on the representative AhTGA gene in peanut cultivars were conducted using transgenic Arabidopsis and soybean hair roots.ResultsThe genome-wide analysis revealed that a total of 20 AhTGA genes were identified and classified into five subfamilies. Collinearity analysis revealed that AhTGA genes lack tandem duplication, and their amplification in the cultivated peanut genome primarily relies on the whole-genome duplication of the diploid wild peanut to form tetraploid cultivated peanut, as well as segment duplication between the A and B subgenomes. Promoter and Protein-protein interaction analysis identified a wide range of cis-acting elements and potential interacting proteins associated with growth and development, hormones, and stress responses. Expression patterns of AhTGA genes in different tissues, under abiotic stress conditions for low temperature and drought, and in response to hormonal stimuli revealed that seven AhTGA genes from groups I (AhTGA04, AhTGA14 and AhTGA20) and II (AhTGA07, AhTGA11, AhTGA16 and AhTGA18) are involved in the response to abiotic stress and hormonal stimuli. The hormone treatment results indicate that these AhTGA genes primarily respond to the regulation of jasmonic acid and salicylic acid. Overexpressing AhTGA11 in Arabidopsis enhances resistance to cold and drought stress by increasing antioxidant activities and altering endogenous hormone levels, particularly ABA, SA and JA.DiscussionThe AhTGA genes plays a crucial role in hormone regulation and stress response during peanut growth and development. The findings provide insights into peanut's abiotic stress tolerance mechanisms and pave the way for future functional studies.
Energy transfer from phycobilisomes to photosystem I at 77 K
Authors: van Stokkum Ivo H. M. , Akhtar Parveen , Biswas Avratanu , Lambrev Petar H. 
Published: November 2023

Phycobilisomes serve as a light-harvesting antenna of both photosystem I (PSI) and II (PSII) in cyanobacteria, yet direct energy transfer from phycobilisomes to PSI is not well documented. Here we recorded picosecond time-resolved fluorescence at wavelengths of 605–760 nm in isolated photosystem I (PSI), phycobilisomes and intact cells of a PSII-deficient mutant of Synechocystis sp. PCC 6803 at 77 K to study excitation energy transfer and trapping. By means of a simultaneous target analysis of the kinetics of isolated complexes and whole cells, the pathways and dynamics of energy transfer in vitro and in vivo were established. We establish that the timescale of the slowest equilibration between different terminal emitters in the phycobilisome is ≈800 ps. It was estimated that the terminal emitter in about 40% of the phycobilisomes transfers its energy with a rate constant of 42 ns−1 to PSI. This energy transfer rate is higher than the rates of equilibration within the phycobilisome – between the rods and the core or between the core cylinders – and is evidence for the existence of specific phycobilisome-PSI interactions. The rest of the phycobilisomes remain unconnected or slowly transferring energy to PSI.
Predicting online information seeking on Douyin, Baidu, and other Chinese search engines among gynecologic oncology patients: a cross-sectional study
Authors: Xiong Wenli , Li Xiaohong , Han Yun , He Lijuan 
Published: November 2023

ObjectiveThe rise of online platforms like Douyin, Baidu, and other Chinese search engines has changed how gynecologic oncology patients seek information about their diagnosis or condition. This study aimed to investigate the factors associated with information seeking among these patients and to evaluate their predictive performance.MethodsA cross-sectional study was conducted among 199 gynecologic oncology patients at a single hospital in China. The patients’ demographic characteristics and scores on the State–Trait Anxiety Inventory (STAI-S and STAI-T) and the Hospital Anxiety and Depression Scale (HADS-A and HADS-D) were compared between those who sought information online and those who did not. Logistic regression analyses and receiver operating characteristic (ROC) curve analyses were performed.ResultsThe patients’ age, marital status, STAI-S scores, and HADS-A scores were significantly associated with online information seeking. The combined model that included these factors showed good predictive performance with an area under the ROC curve of 0.841.ConclusionThe combination of demographic and psychological factors can be used to predict the likelihood of gynecologic oncology patients seeking information online. These findings can help healthcare providers understand their patients’ information-seeking behaviors and tailor their communication strategies accordingly.
What can we learn from COVID-19?: examining the resilience of primary care teams
Authors: Hughes Ashley M. , Arredondo Kelley , Lester Houston F. , Oswald Frederick L. , Pham Trang N. D. , Jiang Cheng , Hysong Sylvia J. 
Published: November 2023

IntroductionThe COVID-19 pandemic continues to place an unprecedented strain on the US healthcare system, and primary care is no exception. Primary care services have shifted toward a team-based approach for delivering care in the last decade. COVID-19 placed extraordinary stress on primary care teams at the forefront of the pandemic response efforts. The current work applies the science of effective teams to examine the impact of COVID-19—a crisis or adverse event—on primary care team resilience.MethodsLittle empirical research has been done testing the theory of team resilience during an extremely adverse crisis event in an applied team setting. Therefore, we conducted an archival study by using large-scale national data from the Veterans Health Administration to understand the characteristics and performance of 7,023 Patient Aligned Care Teams (PACTs) during COVID-19.ResultsOur study found that primary care teams maintained performance in the presence of adversity, indicating possible team resilience. Further, team coordination positively predicted team performance (B = 0.53) regardless of the level of adversity a team was experiencing.DiscussionThese findings in turn attest to the need to preserve team coordination in the presence of adversity. Results carry implications for creating opportunities for teams to learn and adjust to an adverse event to maintain performance and optimize team-member well-being. Teamwork can act as a protective factor against high levels of workload, burnout, and turnover, and should be studied further for its role in promoting team resilience.