bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chinese grand(parenting) encourages a re‐imagining of Canada's immigration regime
Authors: Wang Chen J. , Hari Amrita 
Published: October 2023

AbstractCanada's migration regime prioritizes the admission of young skilled immigrants while restricting elderly immigrants constructed as non‐contributing dependents. We review and compare the current pathways to family reunification for elderly immigrants. Based on interviews with 16 Chinese skilled immigrant mothers and eight sponsored (grand)parents in Edmonton and Ottawa, we highlight the contributions made by elderly Chinese immigrants to social reproduction, intergenerational cultural preservation, and various forms of transnational support. We also reveal the financial and emotional costs incurred by young skilled immigrants who sought to sponsor their elderly parents for permanent residency. Based on these rich conversations, we propose two policy recommendations to reduce the pressure on sponsoring family members and create opportunities for elderly immigrants to access gainful employment.
Improved positional accuracy of dental implant placement using a haptic and machine‐vision‐controlled collaborative surgery robot: A pilot randomized controlled trial
Authors: Shi Jun‐Yu , Liu Bei‐Lei , Wu Xin‐Yu , Liu Min , Zhang Qi , Lai Hong‐Chang , Tonetti Maurizio S. 
Published: October 2023

AbstractAimTo compare the implant accuracy, safety and morbidity between robot‐assisted and freehand dental implant placement.Materials and MethodsSubjects requiring single‐site dental implant placement were recruited. Patients were randomly allocated to freehand implant placement and robot‐assisted implant placement. Differences in positional accuracy of the implant, surgical morbidity and complications were assessed. The significance of intergroup differences was tested with an intention‐to‐treat analysis and a per‐protocol (PP) analysis (excluding one patient due to calibration error).ResultsTwenty patients (with a median age of 37, 13 female) were included. One subject assigned to the robotic arm was excluded from the PP analysis because of a large calibration error due to the dislodgement of the index. For robot‐assisted and freehand implant placement, with the PP analysis, the median (25th–75th percentile) platform global deviation, apex global deviation and angular deviation were 1.23 (0.9–1.4) mm/1.9 (1.2–2.3) mm (p = .03, the Mann–Whitney U‐test), 1.40 (1.1–1.6) mm/2.1 (1.7–3.9) mm (p < .01) and 3.0 (0.9–6.0)°/6.7 (2.2–13.9)° (p = .08), respectively. Both methods showed limited damage to the alveolar ridge and had similar peri‐ and post‐operative morbidity and safety.ConclusionsRobot‐assisted implant placement enabled greater positional accuracy of the implant compared to freehand placement in this pilot trial. The robotic system should be further developed to simplify surgical procedures and improve accuracy and be validated in properly sized trials assessing the full spectrum of relevant outcomes.
Mediation of meaningful outcomes in early intervention: a commentary on Carruthers et al. (2023)
Authors: Shire Stephanie , Shih Wendy 
Published: October 2023

Across the spectrum of behavioral, naturalistic developmental behavioral, and developmental/relational interventions for young children with autism, there has been limited empirical testing of the mechanism of these early intervention approaches. Testing mediation provides insights to the how or why the intervention condition may be preferred in comparison to a control in the population sample (Kraemer, American Journal of Psychiatry, 2016, 173, 672). Combined with an understanding of moderation (for whom or under what conditions), we move toward a better understanding of how to personalize interventions to build on strengths and maximize skill gains for autistic children. Yet, to date there are very few published texts of mechanism in early intervention for young children with autism. This commentary explores a recently published article by Carruthers et al (2023) and examines themes and considerations for measurement of intervention outcomes and mediators.
European equity markets volatility spillover: Destabilizing energy risk is the new normal
Authors: Huszár Zsuzsa R. , Kotró Balázs B. , Tan Ruth S. K. 
Published: October 2023

AbstractWhile energy risk is increasingly recognized as a systemic risk, there is limited comprehensive analysis of the risk propagation in regional contexts. In this study, we examine oil and natural gas price changes and shocks in relation to equity market returns and volatility for 24 European Economic Area (EEA) countries. In addition to traditional panel regressions, we also deploy the Diebold‐Yilmaz (2014) spillover index for a closed network analysis. We differentiate in the cross‐section across the core EU block, PIIGS countries, EU enlargement countries joining after 2004, and other non‐EU countries, to provide insights into the ongoing debates on the European energy market stability. While we find evidence of the manifestation of energy risk throughout the sample period, we find that until 2019 the primary sources of volatility spillover in the EEA economic network arose from economic or political uncertainty. Energy risks, measured by large crude oil and natural gas price shocks also significantly contributed to equity market volatility, with increasing volatility risk arising from natural gas, a green labelled energy source after 2019. Last, we show that CEEC equity markets are more sensitive to oil and natural gas price shocks when domestic currencies depreciate against the Euro.
First report of Neopestalotiopsis clavispora causing foliar disease of rubber (Hevea brasiliensis) in the Philippines
Authors: Solpot Tamie C. , Villanueva Jill D. , Abello Meynard P. , Zuñiga John Benrich M. 
Published: October 2023

AbstractIn September 2022, a leaf spot disease, which later coalesce and result into leaf defoliation of rubber was observed in Tungawan, Zamboanga Sibugay, Philippines. Morpho‐cultural and molecular characterization identified the pathogen as Neopestalotiopsis clavispora. Koch's postulates were fulfilled confirming its pathogenicity to rubber seedlings. To the best of our knowledge, this is the first report of N. clavispora infecting rubber in the Philippines.
Gastric pH and serum gastrin concentration in age‐matched healthy dogs and dogs with chronic kidney disease
Authors: Grady Kylie , Ernst Eli , Secoura Patricia L. , Price Josh , Birkenheuer Adam , Vaden Shelly L. , Lidbury Jonathan , Gould Emily , Steiner Joerg M. , Tolbert M. Katherine 
Published: October 2023

AbstractBackgroundGastric hyperacidity and hypergastrinemia are purported to cause gastric ulceration in dogs with chronic kidney disease (CKD); however, no published studies have evaluated gastric pH with serum gastrin concentrations in dogs with CKD.HypothesisTo compare mean intragastric pH, mean percent pH distribution, and serum gastrin concentrations in dogs with CKD to age‐matched, healthy dogs. We hypothesized there would be no difference in mean gastric pH or serum gastrin between groups.AnimalsThirteen dogs with CKD; 10 aged‐matched healthy dogs.MethodsProspective, case‐control study. Serum chemistry, complete blood count, urinalysis, and serum gastrin concentrations were evaluated in all dogs before radiographic‐assisted gastric placement of a pH capsule. Forty‐eight‐hour continuous gastric pH monitoring was performed in all dogs. Serum gastrin concentration, mean pH, and mean percentage time that gastric pH was strongly acidic (pH <1 and pH <2) were compared between groups using a repeated measures mixed‐model ANOVA.ResultsNo significant differences were observed between groups for any pH measurements, including mean ± SD gastric pH (CKD, 2.37 ± 0.87; healthy, 2.39 ± 0.99; P > .05). Serum gastrin concentrations were not significantly different between groups (median [range]: CKD, 10.5 ng/dL [<10‐17.1]; healthy, 10.9 ng/dL [<10‐15]; P > .05).Conclusions and Clinical ImportanceOur client‐owned dogs with CKD did not have lower gastric pH or higher serum gastrin concentrations compared to healthy dogs. Our results suggest that prophylactic gastric acid suppression in dogs with CKD is not warranted unless other clinical indications for use are present.
Population biology of the endangered land crab Johngarthia lagostoma (H. Milne Edwards, 1837) in the Trindade Island, Brazil: Identifying crucial areas for future conservation strategies
Authors: João Marcio Camargo Araujo , Duarte Rafael Campos , Freire Andrea Santarosa , Kriegler Nicholas , Pinheiro Marcelo Antonio Amaro 
Published: October 2023

AbstractThe life history of insular gecarcinid crabs is divided into a marine larval and an adult phase, adapted to the terrestrial environment. As adults, individuals migrate seasonally to locations near the sea, engaging in reproductive behaviors. Therefore, identifying breeding and recruitment areas is crucial for the conservation of insular gecarcinids, especially for those endangered, such as Johngarthia lagostoma (H. Milne Edwards, 1837). We used sex ratio and sexual dimorphism analyses (body and cheliped size) as well as records of juveniles and adults to describe the population structure of J. lagostoma in the Trindade Island, Brazil. During the reproductive period, several population parameters were compared among sites with different altitudes, comprising two beaches (Andradas and Tartarugas) and two hills (Príncipe, 136 m; and Desejado, 612 m). Overall, males predominated in the population and invested more in body and cheliped growth than females. However, at Andradas Beach, it was observed a similar frequency of adult males and females as well as a small difference in the body size between the sexes. In comparison, the smallest crabs were found at Príncipe Hill. The analyzed population presented a predominance of adults, especially at Andradas Beach (93.1%). Compared to the other sites sampled, Príncipe Hill showed the highest abundance of juveniles (males: 22.2%; females: 40.8%). Thus, Andradas Beach was considered a reproductive site, while Príncipe Hill had the highest density of juveniles, which makes them priority areas for the conservation of J. lagostoma in Trindade Island.
PdeERF114 recruits PdeWRKY75 to regulate callus formation in poplar by modulating the accumulation of H2O2 and the relaxation of cell walls
Authors: Zhang Yan , Cai Guanghua , Zhang Keai , Sun Huanxi , Huang Liyu , Ren Wenyu , Ding Yiwei , Wang Nian 
Published: October 2023

Summary
Callus formation is important for numerous biological processes in plants. Previously, we revealed that the PdeWRKY75–PdeRBOHB module positively regulates hydrogen peroxide (H2O2) accumulation, thereby affecting callus formation in poplar.
In this study, we identified and confirmed a transcription factor, PdeERF114, that interacts with PdeWRKY75 both in vitro and in vivo. Gene expression analysis identified both PdeRBOHB and PdeEXPB2 as downstream genes of PdeERF114 and PdeWRKY75. Overexpression (OE) and reduced‐expression (RE) transgenic poplar lines for these four genes were generated, and the observation of callus formation was also performed in all plant materials.
We demonstrated that PdeERF114 and PdeWRKY75 formed a protein complex and that this complex could bind W‐Box motifs in the promoters of PdeRBOHB and PdeEXPB2, thereby positively regulating the expression of PdeRBOHB and PdeEXPB2. The OE/RE transgenic lines for these four genes also showed enhanced/reduced callus formation.
Overall, we revealed a novel gene regulatory network for the regulation of callus formation in plants that involves four genes and regulates callus formation through two pathways: the accumulation of H2O2 in explants and the relaxation of cell walls. In the future, the four genes could be used to enhance transformation effectiveness in genetic engineering.
Prevalence and correlates of very rapid repeat pregnancy: Pregnancy Risk Assessment Monitoring System, United States, 2009–2020
Authors: Luff Amanda , Menegay Michelle , Gallo Maria F. 
Published: October 2023

AbstractBackgroundMost rapid repeat pregnancies, defined as those occurring within 18 months of a previous birth, are unintended. These pregnancies are associated with later initiation of prenatal care and are more common among people with lower socio‐economic status and among racially and ethnically minoritised populations.ObjectivesTo assess prevalence and correlate pregnancies occurring in the immediate period after a live birth in the United States, using the Pregnancy Risk Assessment Monitoring System (PRAMS).MethodsWe assessed data from the 2009–2020 PRAMS, a population‐based survey of perinatal maternal characteristics of mothers of liveborn infants in US locations. We assessed pregnancies reported during the immediate postpartum period (approximately 2–6 months post‐delivery), and term this ‘very rapid repeat pregnancy’ (VRRP). We assessed the adjusted prevalence of VRRP from 2009 to 2020. From 2016 to 2020, we calculated adjusted prevalence ratios (aPR) with 95% confidence intervals (CI) for maternal characteristics.ResultsThe adjusted prevalence of VRRP ranged from 0.38% (95% CI: 0.29, 0.48) in 2009 to 0.76% (95% CI: 0.61, 0.91) in 2020. Demographic characteristics associated with VRRP included younger age, lower educational attainment, and being unmarried. Black mothers had a higher prevalence of VRRP compared to white mothers. Mothers who attended a healthcare visit in the 12 months preconception had a lower prevalence of VRRP as did mothers who attended a postpartum check‐up, compared to their counterparts without these visits. Among those receiving prenatal care, mothers whose prenatal healthcare provider asked about postpartum contraception birth had a lower prevalence of VRRP, compared to those not asked about postpartum contraception.ConclusionsVRRP appeared to increase over time in 2009–2020. Mothers who are younger, Black, have lower educational attainment, or who did not attend healthcare visits before or after pregnancy had a higher prevalence of VRRP and may comprise a population who would benefit from additional family planning resources.
A new hope for vascular access, modifying a new technique: The Surfacer® Inside‐Out Access Catheter System and sharp recanalization
Authors: Rau Melissa , Alba Esther , Valcarcel Joana , Landa Jaid , Sandoval Diego 
Published: October 2023

AbstractVascular access (VA) for hemodialysis (HD) continues to be the keystone of the technique's success. The first option should be an arteriovenous fistula (AVF) or arteriovenous graft (AVG), although in some scenarios the only possibility available is a central venous catheter, which could lead to an increase in vascular complications such as thoracic central vein occlusion (TCVO), becoming a challenge for physicians. If conventional therapies cannot be performed, a new device called the Surfacer® Inside‐Out® Access Catheter System (IOA) (Bluegrass Vascular Technologies, San Antonio, TX, USA) arises as a promising alternative. Here we describe the case of a patient in which we performed a combination of two techniques: sharp recanalization plus Surfacer® Inside‐Out® Access Catheter System.
Satisfaction of basic psychological needs and European entrepreneurs' well‐being and health: The association with job satisfaction and entrepreneurial motivation
Authors: Padilla‐Angulo Laura , Lucia‐Casademunt Ana M. , Gómez‐Baya Diego 
Published: October 2023

PurposeWe examine an integrative model associating entrepreneurial motivation and job satisfaction with basic psychological needs satisfaction and the psychological well‐being (PWB) and health problems of European entrepreneurs. In contrast with previous literature that focuses mainly on hedonic well‐being, this study examines well‐being by using a eudaimonic perspective and the link between entrepreneurial motivation and entrepreneurs' PWB.Design/methodology/approachBased on the self‐determination theory (SDT) and using structural equation modeling, this study examines a European representative sample composed of 7,878 entrepreneurs from the sixth European Working Conditions Survey (6th EWCS; Eurofound [2015] database).FindingsThis study finds a positive relationship between the satisfaction of the need for autonomy and competence and opportunity motivation, which in turn is positively associated with job satisfaction. This study also finds that need satisfaction is positively associated with entrepreneurs' PWB and job satisfaction, which in turn is positively associated with entrepreneurs' PWB and health. Results highlight the relevance of SDT, opportunity motivation, and job satisfaction to understanding entrepreneurs' PWB and health.OriginalityTo the best of our knowledge, this is the first integrative model relating satisfaction of basic psychological needs (autonomy and competence) and diverse individual outcomes related to work (job satisfaction, PWB, and health) of European entrepreneurs by considering entrepreneurial motivation. This study examines a large and representative European sample, in contrast with previous research focusing on the Anglosphere nations.
Establishing hematologic and biochemical reference intervals for the endangered Lear's macaw (Anodorhynchus leari)
Authors: da Costa Machado Paula , da Motta Lima Caio Filipe , Ramos Patrícia Locosque , Chagas Carolina Romeiro Fernandes , Borges Salgado Paula Andrea 
Published: October 2023

AbstractBackgroundThe Lear's macaw, Anodorhynchus leari (Psittaciformes, Psittacidae), is an endemic species of the Caatinga region in Brazil. With only a few free‐living individuals that are constantly under threat from agricultural clearance, hunting, and trapping, A. leari is classified as “endangered” by the Red Book of Endangered Brazilian Fauna and by the International Union for Conservation of Nature. At the same time, the number of individuals in captivity has been growing due to the efforts of biologists and veterinarians in zoological parks to reproduce this species. Thus, having species‐specific reference intervals for hematologic and biochemical measurands is essential to assess the health status of these captive and wild birds and guarantee their reproductive success.ObjectivesThis study aimed to determine reference intervals for hematologic and biochemical measurands for A. leari kept in captivity in São Paulo Zoo, Brazil.MethodsBlood samples from 32 individuals were selected based on inclusion and exclusion criteria. Only samples from adults and healthy individuals were included in this study. All statistical analyses were performed according to the American Society for Veterinary Clinical Pathology guidelines and available literature.ResultsReference values and reference intervals were determined for the captive population of Anodorhynchus leari.ConclusionsThis is the first study to establish reference intervals for A. leari, which can aid the veterinarian in the interpretation of laboratory test results, helping in the diagnosis, treatment, and monitoring of diseases of this endangered species of Brazilian fauna.
Transcription factors SltA and CrzA reversely regulate calcium homeostasis under calcium-limited conditions
Authors: Dai Mengyao , Du Wenlong , Lu Ling , Zhang Shizhu 
Published: October 2023

ABSTRACT

The maintenance of cellular calcium homeostasis in response to calcium supply is required for a wide range of physiological processes in fungi, including growth, development, and virulence. In
Aspergillus
, the transcription factor CrzA is involved in efficient regulation of calcium homeostasis, especially under calcium-repleted conditions. However, the transcriptional regulatory mechanisms under calcium-limited conditions remain unclear. Here, we found that a highly conserved transcription factor, SltA, known for its roles in salt tolerance and azole resistance, can confer adaptation to calcium-limited conditions in
Aspergillus fumigatus
. Loss of
sltA
caused severe growth defects with abnormal expressions of calcium metabolism-related genes, which resulted in a decreased cytosolic calcium transient under calcium-limited conditions, while the addition of calcium rescued all defective phenotypes in the Δ
sltA
mutant. Moreover,
A. fumigatus
SltA undergoes a proteolysis modification that depends on SltB, a chymotrypsin-like serine protease. Strikingly,
A. fumigatus
SltA plays a reverse role to CrzA in the regulation of expressions of calcium metabolism-related genes. Deletion of
crzA
significantly suppressed the growth defect of the Δ
sltA
mutant. Deletion of
sltA
led to highly expressed CrzA with a constant nuclear location under calcium-limited conditions, demonstrating that there exists a mutually restricted network regulated by SltA and CrzA to adapt to calcium-limited conditions. Electrophoretic mobility shift assays and chromatin immunoprecipitation sequencing revealed that SltA directly binds to a subset of genes involved in calcium metabolism via a conserved motif. These findings expand knowledge on the regulatory circuits of the Ca
2+
signaling pathway.

IMPORTANCE

Calcium ions are ubiquitous intracellular signaling molecules for many signaling pathways regulating the fungal response to stress and antifungal drugs. The concentration of intracellular calcium is tightly regulated in its storage, release, and distribution. CrzA is the best-studied transcription factor that regulates this process under sufficient calcium or other external signals. However, CrzA was excluded from nuclei and then lost transcriptional activation under calcium-limited conditions. The regulators in the Ca
2+
signaling pathway under calcium-limited conditions remain unclear. Here, we identified SltA as a key regulator in the Ca
2+
signaling pathway under calcium-limited conditions, and the underlying mechanisms were further explored in
Aspergillus fumigatus
. These findings reveal a transcriptional control pathway that precisely regulates calcium homeostasis under calcium-limited conditions.
Protein-based vaccine candidate MecVax broadly protects against enterotoxigenic
Escherichia coli
intestinal colonization in a rabbit model
Authors: Upadhyay Ipshita , Parvej Shafiullah M. D. , Shen Yiyang , Li Siqi , Lauder Kathryn L. , Zhang Chongyang , Zhang Weiping 
Published: October 2023

ABSTRACT

There are no vaccines licensed against enterotoxigenic
Escherichia coli
(ETEC), a leading cause of children’s diarrhea and the most common cause of travelers’ diarrhea. Multivalent vaccine candidate MecVax unprecedentedly targets two ETEC enterotoxins (heat-stable toxin, STa; heat-labile toxin, LT) and the seven most prevalent ETEC adhesins (colonization factor antigen, CFA/I, coli surface antigens, CS1–CS6) and has been demonstrated preclinically to protect against STa- and LT-mediated ETEC clinical diarrhea and prevent intestinal colonization from ETEC strain H10407 (CFA/I, STa, LT). However, it is unattested whether MecVax broadly protects against intestinal colonization from ETEC strains producing the other six adhesins (CS1–CS6) also targeted by this product. In this study, we immunized rabbits with MecVax and challenged them with heterogeneous ETEC strains that express CS1–CS6 adhesins to evaluate MecVax’s efficacy against bacterial intestinal colonization, thus providing broad vaccine protection against ETEC infection. Data revealed that rabbits intramuscularly immunized with MecVax developed robust responses to both ETEC enterotoxins (STa, LT) and seven adhesins (CFA/I, CS1–CS6), and when challenged with ETEC isolates expressing CS1/CS3, CS2/CS3, CS4/CS6, CS5/CS6, or CS6 adhesin, the immunized rabbits prevented over two logs (>99%) of bacteria from colonization in small intestines. Additionally, compared to a CFA-toxoid fusion protein, which is another potential ETEC vaccine antigen to target two ETEC enterotoxins and the seven adhesins, MecVax exhibited better protection against ETEC intestinal colonization. These results, in conjunction with the protection data from early studies, evidenced that MecVax is broadly protective, validating MecVax’s candidacy as an effective vaccine against ETEC-associated diarrhea and accelerating ETEC vaccine development.