bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selenium Yeast Mitigates Diquat-Induced Oxidative Stress in Rooster Testicles and Preserves Reproductive Performance
Authors: Xiong Ningna , Liu Sili , Hu Wei , Liu Yamei , Ding Xiang , Wu Bingyan , Ling Yiqiang , Fang Manxin , Liu Ben , Zheng Lucheng , Zheng Wenya 
Published: October 2023

Abstract

Background:Diquat (DQ) is a pro-oxidant that generates free radicals in cells through redox reactions, leading to the induction of oxidative stress. It is widely utilized in agricultural production across many countries. During the processes of growth and reproduction, poultry are particularly vulnerable to oxidative stress. Selenium yeast (SeY) serves as an organic selenium source characterized by high activity and low toxicity, imparting antioxidant effects. The objective of this study was to assess the protective effects of SeY against DQ-induced oxidative stress in rooster testicles and its impact on reproductive performance. A cohort of 60 healthy Kangle yellow roosters participated in this investigation. These roosters were randomly and equally divided into four groups: the control group, the DQ group, the SeY and DQ group, and the SeY group.
Results: The results demonstrated that SeY pretreatment mitigated DQ-induced oxidative damage in the testes. This mitigation encompassed the alleviation of inhibited spermatogenesis, reduced spermatogenic cell abundance, decreased expression of genes related to testosterone synthesis, and amelioration of impaired reproductive performance. Specifically, SeY pretreatment counteracted DQ-induced oxidative stress by activating the Nrf2/HO-1 antioxidant signaling pathway, enhancing the activity of antioxidant enzymes such as catalase (CAT) and total superoxide dismutase (T-SOD), and reducing the concentration of malondialdehyde (MDA). Furthermore, SeY pretreatment attenuated DQ-induced spermatogonia apoptosis by modulating the expression of apoptosis-related genes and proteins, including Bax, Bcl-2, Caspase3, and NF-κB. Additionally, SeY restored the proliferative capacity of spermatogenic cells by promoting the expression of the proliferation-related protein Ki67.
Conclusions: The aforementioned findings signify that SeY effectively safeguards the testes against DQ-induced damage through mechanisms involving the reduction of oxidative stress, inhibition of apoptosis, promotion of proliferation, and the preservation of reproductive performance in roosters. This study lays a solid theoretical foundation for future research aimed at safeguarding the reproductive health of male poultry exposed to agricultural pesticides.
Mechanisms of Intermittent theta-burst stimulation attenuating nerve injury after ischemic reperfusion in rats through endoplasmic reticulum stress and ferroptosis
Authors: Shen Xin-Ya , Zhang Xing-Yu , Han Ping-Ping , Zhao Yi-Ning , Bi Xia 
Published: October 2023

Abstract
Background: Repetitive transcranial magnetic stimulation (rTMS) exerts neuroprotective effects early in cerebral ischemia/reperfusion (I/R) injury. Intermittent theta-brust stimulation (iTBS), a more time-efficient modality of rTMS, improves the efficiency without at least decreasing the efficacy of the therapy. iTBS elevates cortical excitability, and in recent years it has become increasingly common to apply iTBS to patients in the early post-IS period. However, little is known about the neuroprotective mechanisms of iTBS. Endoplasmic reticulum stress (ERS), and ferroptosis have been shown to be involved in the development of I/R injury. We aimed to investigate the potential regulatory mechanisms by which iTBS attenuates neurological injury after I/R in rats.
METHODS: Rats were randomly divided into three groups: sham-operated group, MCAO/R group, and MCAO/R+iTBS group, and were stimulated with iTBS 36 hours after undergoing middle cerebral artery occlusion (MCAO) or sham-operated. The expression of ERS, ferroptosis, and apoptosis-related markers was subsequently detected by western blot assays. We also investigated the mechanism by which iTBS attenuates nerve injury after ischemic reperfusion in rats by using the modified Neurological Severity Score (mNSS) and the balance beam test to measure nerve function.
RESULTS: iTBS performed early in I/R injury attenuated the levels of ERS, ferroptosis, and apoptosis, and improved neurological function, including mNSS and balance beam experiments. It is suggested that this mode of stimulation reduces the cost per treatment by several times without compromising the efficacy of the treatment and could be a practical and less costly intervention.
Awake prone positioning improves ventilation distribution in patients with acute respiratory failure: a prospective physiological study
Authors: Wang Jingjing , Chen Changxing , Zhao Zhanqi , Deng Puyu , Zhang Chenchen , Zhang Yu , Lv Hui , Chen Daonan , Xie Hui , Wang Ruilan , Road Wujin 
Published: October 2023

Abstract
Background:
Since the beginning of the coronavirus disease 2019 (COVID-19) pandemic, prone positioning has been widely applied for non-intubated, spontaneously breathing patients. Many clinical studies have shown that awake prone positioning (APP) can reduce the intubation rate and help improving the prognosis of patients with acute hypoxemic respiratory failure (ARF), but its physiological mechanism is still unknown. In our study, we aimed to evaluate the effect of APP on lung ventilation in patients with moderate to severe ARF, trying to reveal the effects on ventilation distribution and oxygenation in non-intubated patients.
Measurements:
The study included patients with ARF (PaO2/FiO2 < 200mmHg or SpO2/FiO2 < 235) treated with high-flow nasal oxygen (HFNC). EIT measurements were performed in the supine position (T0), 0.5 h after the start of APP (T1) and at 0.5 h returning to supine position after the APP (T2).
Results:
Based on EIT measurements, it was observed that the distribution of ventilation was more uniform after prone positioning, and prone positioning significantly improved the ventilation of dorsal regions. Ventilation redistributed from the ventral to dorsal area (48.77% vs 54.79% vs 50.34%, P=0.030), global inhomogeneity (GI) index decreased significantly after the end of prone positioning therapy (48.17% vs 42.60% vs 28.21%, P=0.049), regional ventilation delay (RVD) changes are not obvious (5.24% vs 4.47% vs 4.35%, P=0.794).
Conclusions:
Awake prone positioning can improve ventilation distribution and homogeneity of lung ventilation assessed by electrical impedance tomography in non-intubated patients with acute respiratory failure.
The central expression of connexin 36 and 43 in rat animal models of trait and state anxiety
Authors: Alizadeh Mohammadreza , Motaghi Sahel , sirchi Mahya Moradi , Abbasnejad Mehdi , Mahani Saeed Esmaeili 
Published: October 2023

Abstract
Purpose
There are few studies on the role of gap junctions in anxiety. The Gap junctions are intercellular channels and their subunit is connexin (CX). The specific isoforms of connexins for neurons and astrocytes are (CX36) and (CX43). Here, we examined the gene expression of these connexins in animal models of trait and state anxiety.
Methods
The animal grouping was as follows: 1) control group, 2) trait anxiety group in which the rats were placed in the elevated plus maze (EPM) 3) diazepam+ trait anxiety group 4) state anxiety group, in which the animals were placed in the (EPM) after tolerating 120 minutes of isolation 5) diazepam + state anxiety group. Using a real time PCR technique, we examined the gene expression of (CX36) and (CX43) in the ventral hippocampus (v Hip), basolateral amygdala (BLA), and medial prefrontal cortex (m PC).
Results
Data showed that the anxiety of animals in the state anxiety group was significantly higher than the trait anxiety group (p < 0.05). In the state anxiety group, gene expression of (CX36) was lower in (m PC) and (BLA) compared to the control and trait anxiety groups (p < 0.01). The expression of (CX43) in (BLA) and (v Hip) was also lower than in the control and trait anxiety groups.
Conclusions
This study shows (CX36) and (CX43) reduction in the mentioned structures increases anxiety and the role of these connexins in the state anxiety is more prominent than trait anxiety.
Mechanism of Bushen Guchi Pill in the Treatment of Periodontitis Based on Network Pharmacology
Authors: Yang Lina , Zhai Yuhong , Gao Ying , Shan Qiusheng , Chen Li 
Published: October 2023

Abstract
Periodontitis is an oral disease caused by chronic inflammation, which not only damages the periodontal tissue structure but also is associated with some systemic diseases. Clinical treatment is only through symptomatic treatment and cannot fundamentally treat the disease. Bushen Guchi Pill (BGP), as a compound arrangement of conventional Chinese medication, incorporates a positive therapeutic effect on periodontitis, however, the helpful component is unclear. The purpose of this study was to explore the mechanism of BGP in periodontitis treatment based on the Gene Expression Omnibus (GEO) database, network pharmacology, and molecular docking technology. The beneficial components and drug targets of BGP were screened out from the Traditional Chinese Medicine System Pharmacology (TCMSP) database. Search for disease targets of periodontitis from the DisGeNET database, Gene Cards database, and GEO database. The disease target and drug action target overlap as candidate targets. The "Drug-Active composition-Candidate target" network and protein-protein interaction (PPI) network were constructed by Cytoscape software, and the candidate targets were subjected to Gene Ontology (GO)and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Finally, molecular docking was performed by AutoDockVina and PyMOL.189 active compounds of BGP, 283 presumed drug targets of active ingredients, and 1056 periodontitis-related disease targets were identified and 89 candidate targets between BGP and periodontitis were found. IL-1β, IL-6, TNF, PTGS2, DPP4, ACHE, PPARG, CCL2, IL-1α, and PTGS1 were identified as core targets. Quercetin, Kaempferol, Luteolin, Naringenin, Wogonin and Formononetin are the core composition of the drug. The main pathways in BGP treating periodontitis include the IL-17 signaling pathway and TNF signaling pathway. This experiment clarified for the first time the active compositions, therapeutic targets, and action pathways of BGP in treating periodontitis, which provided a theoretical basis for the clinical treatment of BGP in the treatment of periodontitis.
Transthyretin-related familial amyloidosis polyneuropathy with spinal cord damage: A case report
Authors: Liu Hailin , Huang Chao , Du Yanjiao , Liu Jiacheng , Ren Xiangyang , Wang Huilin , Ye Jingna , Zhou Haitao , Duan Zhihui 
Published: October 2023

Abstract
Transthyroxin protein-related familial amyloidosis polyneuropathy is an autosomal dominant genetic disease caused by TTR gene mutation. Peripheral and autonomic nerve damage is the main disease. With the progression of the disease, heart, lung, kidney, eye and other organs are often involved. TTR gene c.148G > A (P.Vir30met) mutation is more common among known mutations, but this type of FAP is rarely involved in the spinal cord. A case of TTR-FAP with spinal cord damage was reported in this paper. This case is A late-onset sporadic patient with peripheral neuropathy with typical sensorimotor and autonomic nerve damage, combined with multiple site damage of heart, kidney, heart failure, and spinal cord injury. Color cardiac ultrasonography showed typical FAP cardiomyopathy, and gene test showed heterozygous mutation of exon 2 c.148G > A (P.AL30met). TTR-FAP with myelopathy was diagnosed. TTR-FAP is a rare disease, especially when combined with spinal cord symptoms, it is difficult to diagnose early. Echocardiography is a non-invasive, repeatable method to evaluate cardiac characteristics and function of cardiac amyloidosis, and genetic testing plays a crucial role in diagnosis.
Predictive Value of Red Cell Distribution Width in Prognosis of Patients with Anterior ST-Elevation Myocardial Infarction and Relationship with Hospital Mortality
Authors: Bodagh Haleh , Banisefid Erfan , Yousefzadeh Asma , Bodagh Mohammad bagher , Moghaddamziabari Seyedehyasmin , Sakhaei Arshiya , Khiavi Shima Mosadeghi , Boudagh Shabnam , Ghanivash Amin 
Published: October 2023

Abstract
Introduction and objective: The clinical diagnosis of acute myocardial infarction (MI) requires a rigorous assessment of the patient's medical history and with evidence of myocardial necrosis obtaind indirectly using biochemical, electrocardiographic, or imaging methods. Normally, the disorder of the blood supply to the coronary arteries and its disproportion with the needs of the myocardium leads to ischemia, hypoxia and finally necrosis of the myocardium. In almost all patients, the vascular pathology is atherosclerosis, usually associated with thrombosis and obstruction of one of the coronary arteries, and considering the increasing prevalence of ST-elevation myocardial infarction (STE-MI) and of healthcare costs and its clinical consequences, and the involvement various factors in the diagnosis of Ant patients STEMI In this study, we intend to investigate the diagnostic value of RDW in Ant STEMI patients as well as the effect of red cell distribution width (RDW) level on hospital and one-year mortality of patients diagnosed with Ant-STEMI who underwent primary angioplasty in Shahid Madani Hospital, Tabriz University of Medical Sciences so that we can evaluate them. The impact of the RDW level on the clinical outcome of these patients was determined and the necessary treatment decisions were made to improve the treatment outcome of these patients.
Materials and Methods: With referring to the archive of Shahid Madani Tabriz Hospital heart Center, the list of patients diagnosed with Ant-STEMI in Shahid Madani Hospital of Tabriz University of Medical Sciences was compiled and assembled, in 2018-2019 underwent primary angioplasty. The inclusion and exclusion criteria were included in the study and reviewed.
Results: In the conducted study, the amount of RDW with one-year mortality and non-hospital mortality was determined. There was no significant relationship, however, with hospital complications such as heart failure and cardiogenic shock. Angio hematoma, gastrointestinal bleeding and stent thrombosis had a significant relationship.
Conclusion: We found that RDW is not a reliable prognostic factor for mortality in ant-STEMI patients.
Development of Chemical and Mechanical Acceleration Stress Test Method for PEMFC Polymer Membranes
Authors: Oh Sohyeong , Lim Daehyeon , Han Yoohan , Lee Seung-tae , Yoo Dong-geun , Park Kwon Pil 
Published: October 2023

Abstract
To improve the durability of polymer electrolyte membrane fuel cell polymer membranes, it is important to evaluate their durability in a short time. In 2016, the U.S. Department of Energy (DOE) presented the AST protocol, a degradation method that simultaneously performs electrochemical and mechanical degradation. The AST protocol has several problems such as long evaluation time, electrode degradation due to open circuit voltage changes, and difficulty in determining whether the durability of the polymer membrane is electrochemically or mechanically weaker. Based on the DOE accelerated stress test protocol, we supplied O2 instead of air to the cathode, increased the drying time, distinguished and improved electrochemical/mechanical evaluation methods. The improved AST protocol reduced the evaluation time of the Nafion XL membrane electrode assembly by approximately 3 times or more, and the degradation of the electrode catalyst was also reduced by 1/3. In addition, it was confirmed that the polymer membrane exhibited weaker mechanical durability than electrochemical; hence, it was possible to ascertain the cause of the degradation, such that the durability of the polymer membrane can be evaluated more accurately.
Assessing the Impact of Dental Restorative Materials on Fibroblast Cells: An Immunohistochemical and ELISA Analysis
Authors: Duzyol Mustafa , Bayram Pinar , Duzyol Esra , Karamese Selina Aksak 
Published: October 2023

Abstract
Introduction: In this study, our aim was to investigate the effects of restorative materials such as composite, compomer and glass ionomer, which are frequently used in dentistry, on L929 fibroblast cells by evaluating the oxidative stress parameters, pro- and anti-inflammatory cytokines, and apoptosis markers.
Methods: L929 fibroblast cells were cultured, and dental filling materials were applied in two doses (50 and 100 µl). Immunohistochemical staining was performed for experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect the level of TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT.
Results: In the light of the data, the examined dental filling materials were effective on increasing the TGF-beta, IL-10, LPO and CAT levels, and decreasing the TNF-alpha, IL-1-beta, and IL-6 levels. The histological micrographs were also support the issues. When the levels of H-score in Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups.
Discussion: Biocompatibility varies according to the content of the material, the amount of residual monomer, and its solubility. Although all the experimental groups have cytotoxic effects, the least effect is seen in the Omnichroma group.
Functional and mechanistic studies of a phytogenic formulation, Shrimp Best, in growth performance and Vibriosis in whiteleg shrimp
Authors: Lee Yi-San , Umam Khotibul , Kuo Tien-Fen , Yang Yu-Liang , Feng Ching-Shan , Yang Wen-Chin 
Published: October 2023

Abstract
Climate change and disease threaten shrimp farming. Here, we conducted a functional and mechanistic study of the administration of a phytogenic formulation, Shrimp Best (SB), to whiteleg shrimp, and assessed their health and growth performance. First, SB was found to increase shrimp body weight by 2.75 to 20.93% and decreased feed conversion ratio by 8–21% compared to control shrimp at 28 days post feeding in aquaria. The data were verified by field trials. Next, we tested the effect of SB on vibriosis in shrimp. SB protected against Vibrio parahaemolyticus as evidenced by survival rate, bacterial load, and hepatopancreatic pathology in shrimp. Finally, we explored the likely mechanism of SB for growth performance and anti-Vibrio protection in shrimp. We initially examined gut microbiota in control shrimp and shrimp fed with SB. The 16S rDNA sequencing data showed that SB increased 6 probiotic genera and decreased 6 genera of pathogenic bacteria in shrimp. Furthermore, Lactobacillus johnsonii, L. reuteri and V. parahaemolyticus were identified from shrimp guts using a serial dilution culture and mass spectrometry. Consistently, polymerase chain reaction data confirmed that SB increased the proportion of L. johnsonii and decreased that of V. parahaemolyticus in shrimp guts. To dissect the relationship among SB, Lactobacillusand Vibrio, we investigated the in vitro regulation of probiotics and pathogenic bacteria by SB. SB at ≥ 0.25 μg/mL promoted growth of L. johnsonii whilst SB at ≥ 200 μg/mL was able to inhibit V. parahaemolyticus growth. Additionally, antagonistic studies revealed that L. johnsonii and its culture supernatant could inhibit V. parahaemolyticus but not vice versa. Furthermore, five anti-Vibrio metabolites, identified from the supernatant of L. johnsonii using liquid chromatography coupled with mass spectrometry were up-regulated by SB. This anti-Vibriomechanism involved destruction of the bacterial membrane. In parallel, we also identified linoleic acid, linolenic acid, and stearic acid as active compounds from SB. Overall, this work demonstrated that SB improved growth performance and vibriosis in shrimp by regulation of gut microbiota, suggesting that SB is a promising phytogenic for shrimp growth and disease control.
Impact of some heavy metal's accumulation in different organs on Fish quality from Bardawil Lake and human health risk assessment
Authors: Zaghloul Ghada Y. , Eissa Hoda A. , Zaghloul Amira Y. , Kelany Mahmoud S. , Hamed Mohamed A. , El-Moselhy Khalid M. 
Published: October 2023

Abstract
Bardawil Lake is a unique aquatic ecosystem that provides a habitat for various fish and other aquatic organisms. This study aimed to analyze the quality of fish species to make sure this lake, like the rest of the other Egyptian lakes, suffers from the deterioration of fish product quality due to the accumulation of some heavy metals (Cd, Pb, Cu, and Zn) in various tissues from fish species that were caught from this lake. 35 fish samples were caught during spring, 2018; from seven different species: Mugil cephalus, Liza auratus, Sparus aurata, Dicentrarchus labrax, Siganus rivulatus, Anguilla angilla, and Solae Solea. The Association of Official Analytical Chemists methods using a spectrophotometer determined the biochemical composition. In contrast, atomic absorption spectrometry was employed to determine the heavy metals expressed by µg/g wet weight. Results exposed that the accumulation of essential micronutrient (Cu, Zn) content was higher than toxic elements (Cd & Pb) in muscles to Zn > Cu > Pb > Cd. Muscles < gills < liver in all metals except Pb muscles < liver < gills. The metals studied in the muscles were lower than those set by the WHO, and the EU standard. The carcinogenic risk with lower allowable limits of 1x10-6 to 1x10-4 in both normal and high consumption groups; target and total target hazard quotients (THQ & HI) in muscles were <1. The biochemical composition level was highest in the liver, except for protein, which was highest in muscle for all fish species. There is no evidence of harmful contaminants in the muscular tissue of the fish sampled from Bardawil Lake. However, customers should be aware that health concerns may be associated with eating too much fish
The potential mechanism of Bu Shen Zhuang Jin Decoction in the treatment of anti-osteoporosis based on mass spectrometry analysis-network pharmacology-molecular docking
Authors: Zhou Shibo , Zhong Wenqing , Yu Xing , Li Wenhao , Zhao Dingyan , Xiong Yang , Ma Yukun , Zhao He 
Published: October 2023

Abstract
Background To investigate the potential mechanism of Bu Shen Zhuang Jin Decoction(BSZJD) in the treatment of anti-osteoporosis based on mass spectrometry analysis-network pharmacology-molecular docking.Methods We used Waters Synapt G2-Si Qtof high-resolution mass spectrometry and Unifi software to analyze the chemical constituents of BSZJD. Querying the targets of ingredients through the Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicines (BATMAN-TCM). GeneCards, OMIM databases were searched for osteoporosis targets. Venny online analysis tool was used to obtain ingredients-disease common targets, construct drug-ingredient-target-disease network by Cytoscape software, and screen core ingredients based on node degree value. Based on disease-ingredient common targets, STRING database and Cytoscape software constructed protein-protein interaction networks and assigned core targets based on node degree value. Metascape was analyzed for GO and KEGG enrichment. The main ingredients and core targets were molecularly docked and the results were visualized by Pymol.Results There are 107 active ingredients in BSZJD. AKT1, ALB, INS, IL6, and TNF were from the 157 targets identified by the protein-protein interaction network. The PI3K-AKT and osteoclast differentiation signaling pathways were identified as possible anti-osteoporosis pathways by the enrichment analysis. Molecular docking confirms that the core ingredients and the core targets have strong binding capability.Conclusion Through mass spectrometry analysis-network pharmacology-molecular docking, we speculate that the BSZJD may play an anti-osteoporotic role by modulating the PI3K/AKT and osteoclast differentiation signaling pathways, which may provide a new idea for the treatment of osteoporosis.