bluuebunny/crossref_metadata_2025_split huggingface dataset:
OsNAC3 regulates seed germination involving abscisic acid pathway and cell elongation in rice
Authors: Huang Chengwei , Zhao Jia , Huang Qianqian , Peng Liling , Huang Zhibo , Li Wenwen , Sun Shan , He Yongqi , Wang Zhoufei 
Published: November 2023

Summary
Seed germination is a critical trait for the success of direct seeding in rice cultivation. However, the underlying mechanism determining seed germination is largely unknown in rice.
Here, we report that NAC transcription factor OsNAC3 positively regulates seed germination of rice. OsNAC3 regulates seed germination involving abscisic acid (ABA) pathway and cell elongation. OsNAC3 can directly bind to the promoter of ABA catabolic gene OsABA8ox1 and cell expansion gene OsEXP4, which consequently activates their expressions during seed germination.
We also find that the expression of OsEXP4 is reduced by ABA during seed germination in rice. OsNAC3 regulates seed germination by influencing cell elongation of the embryo through directly affecting OsEXP4 expression and indirectly ABA‐medicated OsEXP4 expression. The OsNAC3 elite haplotype is useful for genetic improvement of seed germination, and overexpression of OsNAC3 can significantly increase seed germination.
We therefore propose that OsNAC3 is a potential target in breeding of rice varieties with high seed germination for direct seeding cultivation.
Neurogenic myocardial dysfunction post craniopharyngioma resection: A diagnostic dilemma
Authors: Reddy Ashwini , Thappa Priya , Jangra Kiran , Dhandapani Sivashanmugam , Sihag Bhupendra Kumar 
Published: November 2023

AbstractEndoscopic transsphenoidal resection of craniopharyngioma is a commonly used technique. Cerebral vasospasm may occur in nearly 10% of cases leading to adverse neurological outcomes. Cardiopulmonary dysfunction may be seen in patients with severe vasospasm. The literature describing the occurrence of neurogenic stunned myocardium following craniopharyngioma resection in pediatric patients is very sparse. Here, we describe such a case managed with a combination of milrinone (to relieve vasospasm and improve cardiac pump function), noradrenaline (to obtain target blood pressure), and vasopressin (to control urine output). This case report proposes the treatment plan of neurogenic stunned myocardium following vasospasm in pediatric patients.
Kelp breeding in China: Challenges and opportunities for solutions
Authors: Hu Zi‐Min , Shan Ti‐Feng , Zhang Quan‐Sheng , Liu Fu‐Li , Jueterbock Alexander , Wang Gaoge , Sun Zhong‐Min , Wang Xiang‐Yu , Chen Wei‐Zhou , Critchley Alan T. , Ye Nai‐Hao 
Published: November 2023

AbstractBreeding has played an important role in the mariculture and industrialization of kelp in China. However, the current kelp breeding systems in China have encountered some problems relating to germplasm diversity, management, technological innovations, and regional co‐operation. This review summarizes the main challenges, such as top‐down and fragmented management of germplasm libraries, as well as private industry breeding without government regulations, inter‐cultivar accidental admixing and genetic erosion, loss of heterozygosity due to repeated selection and self‐crossing. We outline multiple potential approaches to breed cultivars with improved qualitative/quantitative traits which can be subjected to changing environments, for example: (i) establishing a national germplasm repository to enhance integrative collection and preservation of kelp resources; (ii) planning and implementing kelp breeding programmes according to strategic priorities and goal‐orientations; (iii) optimizing a hybridization‐based breeding pipeline to produce robust cultivars through the introgression of novel alleles and thus the expression of hybrid vigour; (iv) enriching the high‐quality annotated reference genomes and functional analysis of trait‐associated markers/loci to develop DNA‐based breeding technologies; (v) developing new priming‐based (e.g., thermal and disease resistance) bio‐engineering breeding strategies to meet future unpredictable climate change; and (vi) breeding towards an ecological kelp‐microbiome interaction‐based technique to produce cultivars with enhanced performance and adaptability to environmental scenarios. Collectively, the lessons learned from kelp breeding in China and the solutions proposed here may not only potentially improve or re‐invigorate the Chinese kelp industry, but will also assist other developing countries in taking corrective actions to develop a sustainable future kelp farming industry.
Factors affecting birth weight and stillbirth in sows housed in a tropical environment
Authors: Adi Yosua Kristian , Boonprakob Rafa , Kirkwood Roy N. , Tummaruk Padet 
Published: November 2023

AbstractThis study analysed data from a commercial swine herd in Thailand equipped with a free‐farrowing housing system, comprising 17,196 piglets from 1318 litters, to explore the impact of sow and litter characteristics on the piglet birth weight and the incidence of stillbirth. The piglets were classified into four groups based on the total number of piglets born per litter (TB): ≤9 (n = 1434), 10–12 (n = 3232), 13–15 (n = 6537) and ≥16 (n = 5993). Sows were classified into four groups based on parity number: 1, 2–4, 5–7 and ≥8. The piglets were categorized into quartiles based on their birth order ranking: Q1 (n = 4786), Q2 (n = 4143), Q3 (n = 3808) and Q4 (n = 4456). Piglet birth weight was individually measured before colostrum ingestion. On average, TB, the number of live‐born piglets and the incidence of stillbirth were 13.1 ± 3.7, 11.5 ± 3.8 and 6.3%, respectively. Among these litters, 26.6% had TB numbers ≥16. The average piglet birth weight was 1.37 ± 0.36 kg, with 18.3% of piglets weighing ≤1.0 kg at birth. Piglet birth weight was influenced by birth order ranking, as Q4 piglets were found to be heavier than piglets born in Q1–Q3 (p < .001). Moreover, the percentage of piglets with a birth weight of ≤1.0 kg increased from 5.9% in litters with TB of ≤9–25.3% in litters with TB of ≥16 (p < .001). Additionally, primiparous sows had lower piglet birth weights compared to sows with parity numbers 2–4, 5–7 and ≥8 (p < .001). Piglets born in the fourth quartile (Q4) had a higher risk of stillbirth compared to those born in the first (Q1), second (Q2) and third (Q3) quartiles (12.5% vs. 2.2%, 4.1% and 6.6%, respectively, p < .001). The incidence of stillbirth in litters with TB ≥16 was also higher than that in litters with TB ≤9 and 10–12 (p < .05). Furthermore, sows with parity numbers ≥8 had a higher incidence of stillbirth (9.7%) compared to primiparous sows (4.0%, p < .001), sows with 2–4 parity (5.2%, p < .001) and sows with 5–7 parity (7.6%, p = .003). In summary, a high incidence of stillbirth was found in piglets born in the last quartile of litters, in litters with >16 piglets and for sows with parity numbers ≥8. Piglets born in the last quartile of litters were heavier than those born in the first to third quartiles.
Do export demand shocks affect the export quality of multi‐product firms? Evidence from China
Authors: Wang Lei , Huang Xianhai , Sun Qianyun 
Published: November 2023

AbstractWe extend the theoretical model of Antoniades (2015, Heterogeneous Firms, quality, and trade. Journal of International Economics, 95(2), 263–273) to analyze the impact of export demand shocks on the quality of multi‐product firms. Using Chinese micro‐data, we find that positive export demand shocks significantly promote the export quality of multi‐product firms, but the impact decreases as firm productivity increases. In addition to the scale effect and the competition effect, the intensified competition caused by the expansion of export demand will induce firms to skew their export sales towards the best performing products. Our findings elucidate the importance of the resource reallocation effect in the study of trade‐induced export performance.
Heidegger's fundamental ontology and the human good in Aristotelian ethics
Authors: Hacker‐Wright John 
Published: November 2023

AbstractNeo‐Aristotelian ethical naturalists take the concept “human” to be central to practical philosophy. According to this view, practical philosophy aims at a distinctive human good that defines its subject matter. Hence, practical philosophy can survive neither the elimination of the concept nor its subsumption under a more general concept, such as that of the rational agent. The challenge central to properly formulating Aristotelian naturalism is: How can the concept of the human be specified in a way that captures the distinctive role that it is supposed to play in practical philosophy? For the view to be sustained, the concept “human” as it figures in practical philosophy must not designate rationality plus a set of facts that we learn empirically about ourselves and then consider from a detached standpoint of reason. Heidegger's existential analytic offers an approach to addressing the challenge to neo‐Aristotelian naturalism and, therefore, a way of capturing the sui generis human good. I aim to show that concepts of the existential analytic in Being and Time have their origin or parallels in readings of Aristotle and that they capture the distinctive character of the being that we are, exclusively, completely, and as a unity. I argue that fundamental ontology offers a way to grasp the integral unity and irreplaceability of the human that lies at the heart of the neo‐Aristotelian project.
Perioperative daptomycin for prophylaxis of vancomycin‐resistant Enterococcus infection in colonized liver transplant recipients
Authors: Mak Jordan T. , Ha Seung , Perloff Sarah , Knorr John P. 
Published: November 2023

AbstractBackgroundInfection with vancomycin‐resistant Enterococcus (VRE) in liver transplant recipients (LTR) has been associated with extended hospital stays, increased readmission rates, graft failure, and death. A tailored perioperative surgical prophylaxis regimen targeting VRE may reduce postoperative infections in VRE‐colonized patients. This study investigated the outcomes of perioperative daptomycin in this patient population.MethodsThis retrospective, single‐center cohort study included LTR ≥ 18 years old who were VRE‐colonized from June 2018 to November 2022. VRE colonization was identified by a VRE rectal swab screen or a positive VRE culture prior to transplant. Two groups were analyzed: daptomycin versus no daptomycin. All LTR received perioperative piperacillin‐tazobactam for 24 h. If VRE‐colonized, one dose of daptomycin (6 mg/kg) was given pre‐ and postoperatively. Demographics, clinical characteristics, risk factors for VRE infection, and daptomycin dose were collected. The primary outcome was VRE infection at 14 days and 90 days post‐transplant.ResultsThere were 36 VRE‐colonized LTR; 19 received daptomycin and 17 did not. Baseline characteristics and risk factors for VRE infection were similar between groups. More VRE infections occurred in the no daptomycin group within 14 days post‐transplant (24% vs. 0%, p = .04), but at 90 days posttransplant there was no significant difference (29% vs. 16%, p = .43). The average daptomycin dose was 7.1 mg/kg.ConclusionPerioperative daptomycin reduced the rate of VRE infections in VRE‐colonized LTR within 14 days posttransplant but not at 90 days. Future studies should evaluate if higher doses and/or longer duration of perioperative daptomycin can reduce VRE infections beyond 14 days post‐transplant.
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XLG2 and CORI3 function additively to regulate plant defense against the necrotrophic pathogen Sclerotinia sclerotiorum
Authors: Tiwari Ruchi , Garg Kajal , Senthil‐Kumar Muthappa , Bisht Naveen C. 
Published: November 2023

SUMMARYThe membrane‐bound heterotrimeric G‐proteins in plants play a crucial role in defending against a broad range of pathogens. This study emphasizes the significance of Extra‐large Gα protein 2 (XLG2), a plant‐specific G‐protein, in mediating the plant response to Sclerotinia sclerotiorum, which infects over 600 plant species worldwide. Our analysis of Arabidopsis G‐protein mutants showed that loss of XLG2 function increased susceptibility to S. sclerotiorum, accompanied by compromised accumulation of jasmonic acid (JA) during pathogen infection. Overexpression of the XLG2 gene in xlg2 mutant plants resulted in higher resistance and increased JA accumulation during S. sclerotiorum infection. Co‐immunoprecipitation (co‐IP) analysis on S. sclerotiorum infected Col‐0 samples, using two different approaches, identified 201 XLG2‐interacting proteins. The identified JA‐biosynthetic and JA‐responsive proteins had compromised transcript expression in the xlg2 mutant during pathogen infection. XLG2 was found to interact physically with a JA‐responsive protein, Coronatine induced 1 (CORI3) in Co‐IP, and confirmed using split firefly luciferase complementation and bimolecular fluorescent complementation assays. Additionally, genetic analysis revealed an additive effect of XLG2 and CORI3 on resistance against S. sclerotiorum, JA accumulation, and expression of the defense marker genes. Overall, our study reveals two independent pathways involving XLG2 and CORI3 in contributing resistance against S. sclerotiorum.
Efficacy and safety of platelet additive solution‐E stored platelet concentrates
Authors: Spelmink Saskia E. , Jager Suzanne T. , van de Watering Leo , van der Meer Pieter F. , van Gammeren Adriaan J. , Wiersum‐Osselton Johanna C. , Klei Thomas R. L. , Kerkhoffs Jean‐Louis H. ,   PAS‐E Research group
Published: November 2023

AbstractIntroductionIn 2018, platelet (PLT) additive solution‐E (PAS‐E) was introduced. The implementation of PAS‐E was expected to diminish the number of allergic reactions in recipients following a PLT transfusion. Here, we evaluated the efficacy and safety of transfusions with PLTs stored in PAS‐E.Study Design and MethodsAfter implementation of PAS‐E, data were collected from 2 cohorts of patients with hematological disorders as well as oncology patients, receiving PLTs in PAS‐E. A similar patient group in a recent RCT, receiving PLTs in plasma, was used as a historical control group for both cohorts. Endpoints were corrected count increments (CCIs), bleeding scores (only reported in cohort 1), and the incidence of adverse reactions.ResultsIn cohort 1, the mean 1‐h CCI was 14.3 ± 6.9, and the 24‐h CCI was 8.7 ± 5.6. In cohort 2, the 1‐h CCI was 11.6 ± 7.8 and the 24‐h CCI was 7.0 ± 6.1. In the control group, the 1‐h CCI was 15.4 ± 5.5 and 24‐h CCI 8.7 ± 4.8. Bleeding complications of WHO grade ≥2 occurred in 40% of patients in cohort 1 compared to 44% in plasma PCs. The incidence of adverse reactions was 1.2% in the two PAS‐E cohorts, compared to 3.0% in plasma PCs. National hemovigilance data showed a significant reduction in allergic reactions with PAS‐E PC transfusions as compared to plasma PCs with an odds ratio of 0.46 (CI 95% 0.37–0.58).ConclusionThe CCIs of PLTs in PAS‐E were decreased compared to plasma PCs, but clinically acceptable. Allergic transfusion reactions were decreased in PAS‐E PCs compared to plasma PCs.
Translingual Englishes, participatory hip‐hop and social media in Nepal
Authors: Sharma Bal Krishna 
Published: November 2023

AbstractThis article discusses some key functions and features of English in participatory popular culture and social media in Nepal. Analytical attention is paid to how English use in rap battles is entangled with other local languages and semiotic modes to create translingual practices and how online metapragmatic comments about the rap battles give rise to diverse language ideologies. The study shows that by creating their social life offline and online, Nepali young adults project themselves as individuals who have access to niche Englishes via popular culture. English use by Nepali youth functions not only as an instrument to understand Western popular culture but also as a symbolic resource to index a range of such social identities as ‘educated’, ‘civilized’ and ‘competent’. The article concludes by arguing that Nepali young adults create new subjectivities that are often suggestive of social transformation.
A Mechano-Electro-Chemical Coupling Model for Bending Analysis of Single-Stranded DNA Microbeam Biosensors Due to Flexoelectricity
Authors: Tan Zouqing , Feng Yang , Shi Xiaohao , Yue Yanmei , Zhang Nenghui 
Published: November 2023

Abstract
Highly compliant structures such as microbeams can deform substantially in response to interactions between molecules adsorbed on their surface. To understand such systems and improve their detection signals, a mechano-electro-chemical coupling model for mechanical deformations of the microbeams immobilized single-stranded DNA (ssDNA) is established due to flexoelectricity. The governing equations and corresponding boundary conditions of ssDNA microbeams are derived by using the variational principle. The bending deformations of ssDNA microbeams (one for cantilever beam and another for simply supported beam) are derived. The electric potential in the regions inside and outside the ssDNA layer is obtained by linear Poisson–Boltzmann equation for different electrolyte solutions. The analytical expressions to quantify the beam deflection and the potential difference of ssDNA layer are presented. The theoretical predictions are compared with the experimental data to validate the applicability of the present model. Numerical results reveal that the solution types, thickness, and elastic modulus of substrate materials have an obvious influence on the deflections of ssDNA microbeams. Therefore, the present model can help to improve the reading of the bending deformation signal of the microbeam biosensors.
Performance evaluation of the cobas SARS-CoV-2 Duo, a novel qualitative and quantitative assay, for the detection of SARS-CoV-2 RNA
Authors: Su Yang-Di , Lai Chih-Cheng , Lin Tsai-Hsiu , Chen Wei-Cheng , Hsueh Po-Ren 
Published: November 2023

ABSTRACT

Viral load quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a critical measure for monitoring clinical responses of patients with COVID-19. The cobas SARS-CoV-2 Duo (Roche Molecular Systems, Inc.) uses the cobas 6800/8800 platform and is an automated real-time reverse transcription PCR (RT-PCR) assay for the qualitative detection of SARS-CoV-2 RNA (ORF1a and ORF1a/b non-structural regions) in nasal and nasopharyngeal specimens. This assay also performs quantitation of the SARS-CoV-2 RNA level in the collected specimen. The precision, accuracy, and linearity of the cobas SARS-CoV-2 Duo test were assessed and correlated with the cycle threshold (Ct) value of the cobas SARS-CoV-2 assay, and the cobas Liat SARS-CoV-2 and influenza A/B assay (Inf A/B) (cobas Liat) in 201 clinical nasopharyngeal swab specimens. The analytical measurement range of the cobas SARS-CoV-2 Duo has been verified to be 1.0E + 02 IU/mL to 1.0E + 09 IU/mL. The between- and within-run precision evaluation and the linearity results (
R
2
= 0.9961) of the cobas SARS-CoV-2 Duo were acceptable based on the verification criteria. A total of 201 nasopharyngeal specimens were evaluated. The positive, negative, and total agreements between the cobas SARS-CoV-2 Duo and the cobas SARS-CoV-2 assay were 93.5% (157 of 168), 75% (33 of 44), and 92.5% (186 of 201), respectively. The qualitative results (Ct values) from the cobas SARS-CoV-2 and the cobas Liat demonstrated good agreement with the quantitative results from the cobas SARS-CoV-2 Duo assay. In conclusion, the diagnostic performance of the cobas SARS-CoV-2 Duo assay is appropriate. It is a precise, accurate, and sensitive method for the detection of SARS-CoV-2 RNA and is comparable to two qualitative assays.

IMPORTANCE
Quantitative SARS-CoV-2 tests for viral load are necessary to guide patient treatment, as well as to determine infection control measures and policies. Although the real-time RT-PCR assays can report the Ct value to estimate the viral load, there are several serious concerns regarding the use of Ct values. Importantly, Ct values can vary significantly among between- and within-run methods. The diagnostic performance of the cobas SARS-CoV-2 Duo is appropriate. It is a precise, accurate, and sensitive method for the detection of SARS-CoV-2 RNA and is comparable to two qualitative assays (the cobas SARS-CoV-2 and the Liat cobas SARS-CoV-2 and Inf A/B). In contrast, using the Ct value to estimate viral load is not reliable, and utilization of a quantitative detection test, such as the cobas SARS-CoV-2 Duo, to accurately measure the viral load is needed.
Treatments for partial remission of major depressive disorder: a systematic review and meta-analysis
Authors: Gülpen Joost , Brouwer Marlies E , Geurtsen Gert J , van Dis Eva A M , Denys Damiaan A J P , Bockting Claudi L 
Published: November 2023

QuestionPartial remission of major depressive disorder (MDD) is a debilitating and distressing clinical state related to chronicity, morbidity and relapse. Although one-third of patients remit partially, evidence for treatment efficacy is unclear. We provide an overview of treatment options and their efficacy.Study selection and analysisEmbase, PsycINFO, Medline and SCOPUS were systematically searched through February 2023. Included were randomised controlled trials (RCTs) examining any treatment in patients with partially remitted MDD aged 13–65 years, reporting data on severity, remission or relapse.FindingsSeven RCTs examining psychotherapy including 1024 patients were eligible. There were not enough RCTs to examine effects of pharmacotherapy. Psychotherapy was associated with lower depressive symptom severity at post-treatment (Hedges’ g=0.50; 95% CI 0.23 to 0.76), but not at follow-up up to 1 year (Hedges’ g=0.36; 95% CI −0.30 to 1.02) or longer (Hedges’ g=0.02; 95% CI −0.09 to 0.12). Psychotherapy was associated with superior remission rates at post-treatment (OR 2.57; 95% CI 1.71 to 3.87) and follow-up 6 months or longer (OR 1.75; 95% CI 1.21 to 2.53), although not with improved relapse rates at post-treatment (OR 0.17; 95% CI 0.01 to 4.83) or follow-up 6 months or longer (OR 0.46; 95% CI 0.21 to 1.03). Overall methodological quality was poor.ConclusionsPsychotherapy targeting partial remission may be effective in lowering depressive symptom severity and patients may potentially achieve full remission twice as likely. Yet, long-term and prophylactic effects are lacking. Given the risk of chronicity, more high-quality RCTs are needed.PROSPERO registration numberCRD42020188451.