bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of a New Hypotensive Peptide from the Venom of Snake Bothrops jararaca (Bj)
Authors: Basak Ajoy , Carmona Euridice , Au Felicia , Barbarulo Borgheresi Rosa Anna Maria 
Published: December 2023

Background:
Snake venom has become a key source of many bioactive peptides, enzymes,
and toxins associated with blood coagulation and neuronal toxicity. In the past, a number
of bradykinin potentiating peptides have been isolated from snake venom that display hypotensive
activity due to their inhibitory action towards Angiotensin-Converting Enzyme (ACE). Significant
interest has developed to isolate, characterize, and subsequently design peptide analogs as potent
ACE-inhibitors which may find therapeutic applications for the treatment of hypertension and associated
diseases.

Aim:
The aim of this study is to search for new bioactive peptide/s in the venom of the snake Bothrops
Jararaca (Bj).

Objective:
The objective is to isolate and characterize new hypotensive peptides from BJ venom.

Methods:
We examined the venom of Bj which is known to host a range of bioactive peptides.
We have isolated a new peptide (BJ-1) which displayed in vitro potent hypotensive activity. The
peptide was purified via Sephadex G25 column chromatography and RP-HPLC. It was characterized
by mass spectrometry, amino acid analysis, N-terminal sequencing, and chemical synthesis.

Result:
The peptide was identified as an octa-decapeptide with amino acid sequence as DCPSDWSSYEGHCYKPFS
where the two Cys residues are likely present in free state, although they
can form an internal S-S bond upon oxidation. It was fully confirmed by comparing with synthetic
peptide prepared by solid phase chemistry. Both have the same molecular mass (2,108 Da) and
identical bioactivity. Furthermore, we rationalize that BJ-1 may be derived from precursor protein
“Coagulation factor IX/factor X binding protein (CF-IX/X-BP)” by proteolytic cleavage at the Nterminus
of its B-chain within the sequence KPFS18↓E19PKN. This cleavage site contains the recognition
motif of enzyme PCSK8 (Proprotein Convertase Subtilisin Kexin8) also known as Subtilisin
Kexin Isozyme 1 (SKI-1) or Site 1 Protease (S1P). Despite this observation, using a synthetic
peptide encompassing the proposed cleavage site and recombinant PCSK8 enzyme, we found that
the enzyme responsible for generation of BJ-1 is not PCSK8. Further studies will be needed to
identify the associated enzyme and fully characterize the pharmacological and biological properties
of the peptide.

Conclusion:
Our study revealed the presence of a novel hypotensive octa-decapeptide in the venom
of the snake Bothrops jararaca. It is likely derived from the A-chain of protein CF-IX/X-BP
via proteolytic cleavage at the N-terminus by a protease yet to be characterized.
Is SiC a Predominant Technology for Future High Power Electronics?: A Critical Review
Authors: Fletcher A.S. Augustine , Nirmal D. , Ajayan J. , Murugapandiyan P. 
Published: December 2023

:
Due to the magnificent properties of Silicon Carbide (SiC), such as high saturation
drift velocity, large operating temperature, higher cut-off and maximum frequency (fT and fmax),
high thermal conductivity and large breakdown voltages (BV), it is desirable for high power
electronics. With the latest advancements in semiconductor materials and processing technologies, diverse high-power applications such as inverters, power supplies, power converters and
smart electric vehicles are implemented using SiC-based power devices. Especially, SiC
MOSFETs are mostly used in high-power applications due totheir capability to achieve lower
switching loss, higher switching speed and lower ON resistance than the Si-based (Insulated
gate bipolar transistor) IGBTs. In this paper, a critical study of SiC MOSFET architectures,
emerging dielectric techniques, mobility enhancement methods and irradiation effects are discussed. Moreover, the roadmap of Silicon Carbide power devices is also briefly summarized.
Application of Machine Learning Predicting Injuries in Traffic Accidents through the Application of Random Forest
Authors: Singh Veer Bhadra Pratap , Hemamalini V. , Muttipati Appala Srinuvasu , Raju SSSV Gopala , Md Shatil Abu Hena , Sharma Abhishek 
Published: December 2023

Background:
The objective of this work is to analyze and predict the harmfulness in
traffic accidents.

Method:
Several Random Forest statistical models are created, in which the predictable variable
(response/ output variable) is the harmfulness of the accident, while the input variables are the
various characteristics of the accident. In addition, these generated models will allow estimating
the influence or importance of each of the factors studied (input variables) concerning the harmfulness
of road accidents so that it is possible to know in which aspects it is more profitable to
work with the objective of reducing mortality from traffic accidents [1].

Results:
In this regard, the predictive algorithm has an out-of-bag error of 26.55% and an overall
accuracy of 74.1%. Meanwhile, the local accuracy of the mildly wounded class is 66.1% compared
to 81.4% of the dead and severely wounded class, which, as mentioned, has higher prediction
reliability.

Conclusion:
Finally, it is worth noting the enormous usefulness of the Random Forest machine
learning technique, which provides very useful information for possible research or studies that
may be carried out. In the specific case of this work, through the use of the R programming language,
which in turn presents a wide range of freely accessible utilities and functions with which
it may be interesting working, it has generated results of great value for this area of activity, important
to society as road safety.
Investigation on Cross-correction of Cystinosis through Genetically
Engineered Cells Secreting Cystinosin
Authors: Graceffa Valeria 
Published: December 2023

Background:
Cystinosis is a rare inherited lysosomal storage disease (LSD), caused by a mutation in the Cystinosin Lysosomal Cystine Transporter (CTNS). Novel therapies and strategies are needed to improve patients' clinical conditions and quality of life.

Objectives and Methods:
This study assessed whether CTNS can be secreted, and investigated a method to enhance its secretion, by adding a secretion signal to the N-terminus. Human Embry-onic Kidney (HEK) 293 cells were transfected with the resulting construct. The amount of protein secreted was then measured. Uptake by monolayer cultures of cystinotic cells and enzyme activity were also assessed.

Results:
The recombinant protein could effectively be secreted, and the secretion signal slightly further increased its secretion. The secreted recombinant protein was taken up by cystinotic cells, and, after internalization, still retained its biological activity.

Conclusion:
Optimization of the proposed method to increase the secretion of CTNS would pro-vide new insights into the production of recombinant proteins for medical and industrial use. Further identification and screening of alternative signalling peptides and cell types can maximise the secretion and production of recombinant CNTS, to be used as a therapeutic agent in human healthcare.
Compatibility and Thermal Decomposition Kinetics of Hexahydro-1,3,5-
trinitro-1,3,5-triazine with Different Polyester-based Polyurethanes
Authors: Kumar Rajesh , Singh Arjun , Kishor Prateek , Thakur Preeti , Soni Pramod Kumar , Thakur Atul 
Published: December 2023

Backround:
The compatibility study is an important aspect before
pre-formulation of the energetic composites. Any sort of the incompatibility
between the ingredients of the energetic composites greatly affects the safety
and functionality of the energetic composites. Therefore, to develope safer
energetic composites, the compatibility between the different ingredients of the
energetic formulations and their thermal decomposition kinetics is important
study as it is directly linked with the safety and functionality of the energetic composites.

Methods:
The compatibility of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) with
different polyester-based polyurethanes (PUs) were studied by using vacuum stability
tester (VST) and differential scanning calorimetry (DSC) methods as outlined by
North Atlantic Treaty Organisation Standardisation Agreement (STANAG 4147).
The mixture of RDX with polyester-based PUs was cured with MDI (4,4’-methylene
diphenyl diisocyanate), IPDI (isophorone diisocyanate) and TMDI (2,2,4-
trimethylhexamethylene diisocyanate) as curatives to get polyester-based PUs. The
VST measurements were carried out at isothermal temperature of 100℃ for 40 h. For
kinetic study, all the samples were subjected to heat from 25-600℃ at different heating rates under flow rate of nitrogen gas of 40 mL/min

Results:
The VST results revealed that energetic RDX was compatible with all polyester-based PUs and was chemically stable. The thermal decomposition behaviour
was studied by employing thermogravimetric analysis (TGA) and DSC. The DSC results indicated that peak temperature difference (∆Tp) between pure RDX and binary
mixture of RDX and polyester-based PUs i.e., RDX/PE/MDI, RDX/PE/IPDI and
RDX/PE/TMDI were found to be greater than 4℃, indicating that RDX was not compatible with all types polyester-based PUs. The thermal stability in terms of Tmax values of RDX/PE/MDI, RDX/PE/IPDI and RDX/PE/TMDI was found to be significantly reduced as compared to pure RDX. The activation energy obtained by the Kissinger method for RDX/PE/MDI, RDX/PE/IPDI and RDX/PE/TMDI samples was found
to be 220.2, 271.5 and 210.4 kJ/mol, respectively. The experimental results showed
that the values are comparable and in good agreement with the values obtained by
Ozawa method.

Conclusion:
This study provides useful information for selecting polyester
-based PUs as polymeric binder for the preparation of RDX-based energetic
composites.
Recent Scenario of the Indian Healthcare System Post COVID-19
Emergence and in Severely ill Patients
Authors: Mishra Sushant , Mahajan Shushank , Mogha Able , Yadav Abhinav , Dagar Wandeep , Chawla Isha , Dhanawat Meenakshi 
Published: December 2023

Abstract:
The coronavirus disease 2019 pandemic has brought fundamental changes to our priorities
and problems, especially those related to the healthcare sector. The COVID-19 pandemic put
even the world's most advanced healthcare systems to the test and India's healthcare system has been
rattled as well. In this accordance, the Indian government has introduced many new policies and
schemes like free vaccination drive, Atmanirbhar Bharat, and free COVID care under Ayushman
Bharat. With the continuation of COVID-19, problems like Self-medication and hoarding of medicines
among the common population keep on rising and this was due to flu-like symptoms of
COVID-19, miss information on social media, or due to less or no interaction between patients and
doctors as the population is now shifting to telemedicine which offers the benefit of consultation at
their own home. These situations served as a boon to the Pharma sector as stocks of many pharma
companies and industries have been reported to have increased in the past 2 years of the pandemic
due to an increase in demand and manufacturing and consumption of pharmaceutical goods and also
reportedly being increased by alot in coming years. However, this rapid growth of the pharmaceutical
sector is beneficial for pharmacists, businessmen, and others who are directly or indirectly associated
with the healthcare fraternity but in our opinion, it will negatively impact the Indian economy
or can be viewed as a problem which can lead to the downfall of the qualitative aspect of the
pharma sector in the long term.
Merger waves: Are buyers following the herd or responding to structural queues?
Authors: Sonenshine Ralph Mark 
Published: December 2023

While there has been a significant amount of research covering the causes of merger waves, few papers have rank ordered merger waves based on the causes nor sought to determine which rationale leads to higher bidder payouts. This paper seeks to fill this gap by examining a cross section of large mergers across most industries occurring over a 17 year period. I find that merger waves over this period are caused foremost by changing economic and regulatory conditions. It is the behavioral rationale of mispricing, however, that more often leads to higher bidder payouts or merger premiums among acquirers in merger waves.
STUDIES ON FIXED DOSE COMBINATION OF IBRUTINIB AND QUERCETIN SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEMS IN HUMAN CANCER CELL LINES
Authors: Bagri Rashmi , Ravouru Nagaraju 
Published: December 2023

Ibrutinib (IB), irreversibly inhibits Bruton’s Tyrosine Kinase which plays a crucial role in the tumor microenvironment and Quercetin (QC) shown apoptosis induction, angiogenesis inhibition, and anti-proliferative action against several human carcinoma cells. The self nano-emulsifying drug delivery system (SNEDDS) is suitable for the loading of insolubilized oil-based compounds such as Ibrutinib and Quercetin. In the current study IB with QC was formulated into SNEDDS and cytotoxicity was determined by using Human malignant melanoma (A-375) and Human lung adenocarcinoma (A549) cell lines. The optimized loaded formula consisted of Castor oil, Kolliphor® RH 40, and PEG-600. The optimized formulation was evaluated for physical parameters and the results were satisfactory. For cytotoxicity studies MTT assay was conducted for these combinations, IC50 values were calculated for the tested compound. In A-549 adenocarcinoma cell line, the calculated IC50 values (μM) for the test compounds T1 (pure IB+QC) and T2 (IB+QC SNEDDS) were 70.34+ 0.8 µM and 85.46 + 0.93 µM at 24 h study, respectively. In A-375 cancer cell line, IC50 values for the compounds T1 and T2 were 59.52 + 0.87 µM and 88.43 + 1.03 µM for 24 h study, respectively. It was observed that the IC50 of IB-QC loaded SNEDDS was higher than pure drug combination and these enter the cells by active transport and induce cytotoxicity to the cells. The overall results from the studies suggest that IB-QC-loaded SNEDD provided synergistic effects, which could play a significant role in the percentage of cell death.
Molecular Docking Studies to Identify Inhibitors of Norepinephrine Reuptake from Marine Algae
Authors: Razvi Ummehani A. , Kamble Laxmikant H. 
Published: December 2023

Depression is one of the major mental health problems at prevalence now days. It can be characterized by poor concentration, low self-esteem, losing interest in family or social life, feeling tired or fatigue, suicidal thoughts and similar symptoms. There are treatments like psychotherapies, antidepressants and electroconvulsive therapies available, but there is need to identify more effective treatment with lesser side effects. Marine organisms like algae, sponges or corals are been investigated to explore their potential as antidepressants. This article aims to explore the potential of some compounds from marine algae by molecular docking and assessment of pharmacokinetics. Human norepinephrine transporter (hNET) was used as target for this study as this transporter is responsible to reuptake norepinephrine and disturbs chemical balance of brain, which can be a cause for depression. Binding affinity of these compounds was compared with binding affinity of prescribed drug levomilnacipran. From 14 selected compounds, 13 showed higher binding affinity towards hNET. Among all compounds, saringosterone has highest binding score. Pharmacokinetics properties were constructive for most compounds. Compounds showed weaker druglikeness and drugs score but can be optimized to enhance it. Compounds identified as inhibitors of NET can be developed as drug molecules in future or algal source for it can be taken as a food supplement.
Percepções sobre comportamento alimentar compulsivo, imagem corporal e inflamação em indivíduos com esquizofrenia
Authors: Amato Isabel de Andrade , Silva Renato Oliveira , Lorencetti Pedro Gabriel , Vicentin Sérgio Roberto Correa , Prado Thaís Paulino do , Bobbo Vanessa Cristina , Nogueira Guilherme Augusto da Silva , Celeri Eloisa Helena Rubello Valler , Azevedo Renata Cruz Soares de , Velloso Lício Augusto , Dalgalarrondo Paulo , Santos Junior Amilton dos 
Published: December 2023

Objetivo: O Transtorno da Compulsão Alimentar Periódica (TCAP) é altamente prevalente em indivíduos com esquizofrenia. Este estudo tem como objetivo explorar a percepção da compulsão alimentar em pacientes com esquizofrenia e suas associações com marcadores clínicos, psicopatológicos e inflamatórios. Métodos: 45 participantes com queixa de compulsão alimentar foram avaliados por meio da Escala de Compulsão Alimentar Periódica (BES). Medidas clínicas e antropométricas, gordura corporal, dimensões psicopatológicas (PANSS) e um escore de satisfação com a imagem corporal com base nos itens da Body Shape Satisfaction Scale (BSSS) e do Body Shape Questionnaire (BSQ) e marcadores inflamatórios séricos, como IL-1β, IL - 6, IL-10, TNF-α e leptina foram dosados. Resultados: Os testes de correlação de Spearman revelaram correlações positivas entre escores de BES, insatisfação com a imagem corporal e escore PANSS, bem como correlação negativa com IL-10. Modelos de regressão linear múltipla mostraram que os escores de BES, embora não tenham sido suficientes para o diagnóstico de compulsão alimentar, estiveram associados positivamente com escore de insatisfação com a imagem corporal, níveis de IL-1β, IL-6 (ambas interleucinas pró inflamatórias) e negativamente com IL-10 (uma interleucina anti-inflamatória). Conclusão: Nossos achados sugerem que a inflamação crônica e a psicopatologia em indivíduos com esquizofrenia podem estar associados a comportamentos alimentares compulsivos e pior percepção de autoimagem corporal. Além da inflamação, o comer compulsivo pode contribuir para o aumento da mortalidade cardiovascular e piora do estado de saúde desses pacientes.
Analysis of water quality on growth performance bamboo lobster (Panulirus versicolor) in Tanjung Limau, Bontang City, East Kalimantan
Authors: Andriawan Soni , Fahreza Mohammad Adam , David Hermawan , Handajani Hany 
Published: December 2023

Lobster is one of the commodities that have high economic value in the world market. Thus, the export opportunities for lobster commodities are still promising. One area that can produce lobster in Indonesia is Bontang, East Kalimantan. The area which is the sea waters is very potential for lobster rearing cultivation. This study was conducted with the aim of analyzing good water quality in lobster rearing cultivation in Bontang, East Kalimantan. The study used 3 floating net cages by taking water samples 4 times and 5 lobsters each sampling. Water quality samples include physics (color, smell and taste, temperature, brightness), chemistry (pH, DO, nitrite, salinity), biology (coliform), heavy metals (hg, pb, cu). Lobster samples were measured by weight. The results showed that Fsignificant > 0.05 so that the water quality parameters had no effect on growth. The cause of the ineffectiveness of water quality parameters is due to the contamination of waters in Bontang, East Kalimantan.Keywords: Bamboo Lobster; Contamination; Floating Net Cage; Heavy Metal.
Assessment of some heavy metal content in sediments of a mangrove swamp, niger delta, nigeria using applicable ecological risk indices
Authors: Chris Davies Ibienebo , Anyanwu Emeka Donald 
Published: December 2023

Sediments can act as pollutant sinks and a source of pollution in aquatic environments. The surface sediments of the Isaka–Bundu tidal swamp, Niger Delta was assessed for heavy metal content using standard methods and assessment indices. The study was carried out in three stations between January and June 2022. Five heavy metals (lead, copper, cadmium, nickel and zinc) were evaluated while eight assessment indices (Contamination Factor - CF, degree of contamination - DC, Ecological Risk - Er, Potential Ecological Risk - PERI, Pollution Load Index - PLI, Enrichment Factor - EF, Geo-accumulation Index - Igeo and Quantification of Contamination -QoC) were applied. Results showed that cadmium and copper exceeded permissible limits while lead, zinc and nickel were within limits. Stations 1 and 3 recorded significantly higher values indicating anthropogenic influence. The dominant anthropogenic activities observed in the area include maritime and dockyard activities and artisanal crude oil refinery. The assessment indices showed that the sediments were at various levels of heavy metal pollution mainly influenced by copper and cadmium viz: CF: Cu (considerable degree) and Cd (very high degree), DC (very high degree), Er: Cd (high ecological risk) and Cu (very high ecological risk), PERI (significant high ecological risk), PLI (very high level pollution), EF: Pb, Zn, Cu (Station 1) and all Cd (extremely high enrichment), Igeo: Cd (extremely polluted) and QoC: Cu and Cd (anthropogenic source). The sediments were highly polluted with toxic metals, which is detrimental to humans and aquatic biota.Keywords: Heavy metal; Indices; Mangrove; Niger Delta; Sediment.
INOVASI PENGOLAHAN OTAK-OTAK IKAN PATIN PADA UMKM â€œAHMADI KULINERâ€
Authors: Iriani Dian , Hasan Bustari , Alfajriey Rayhand , Ramadhan Tengku Rahmat , Zein Fauzan Fadilah , Sidik Fawwaz Afifah , Berkhaty Ayang Putri , Ananda Dea , Maulani Iffah , Rinanda Khairunnisa Yulia , Yustiani Maulida 
Published: December 2023

UMKM Ahmadi Kuliner adalah salah satu UMKM yang terdapat di Desa Pulau Lawas Kecamatan Bangkinang, Kampar yang memiliki potensi besar dalam pengembangan usaha lokal masyarakat dengan memanfaatkan ikan patin yang merupakan ikan andalan Kabupaten ini. Tujuan dari kegiatan ini adalah untuk membantu mitra UMKM Ahmadi Kuliner di desa Pulau Lawas dengan memberikan pelatihan dalam inovasi pengembangan produk baru berbahan dasar ikan patin berupa otak-otak ikan sebagai makanan sehat. Metode yang digunakan dalam kegiatan ini yaitu penyuluhan tentang manfaat mengkonsumsi daging ikan dan dilanjutkan dengan pelatihan pembuatan otak-otak ikan patin sebagai inovasi baru dalam pengembangan produk jajanan sehat. Sebelum kegiatan dilakukan, peserta yang merupakan mitra UMKM mengisi kuisioner (Pre-test) dan diminta pendapatnya tentang produk yang telah dibuat (Post-test). Pengumpulan data dilakukan secara kualitatif dan dibahas sesuai dengan literatur. Kegiatan penyuluhan dan pelatihan pada UMKM Ahmadi Kuliner berjalan sukses dan lancar, dimana peserta sangat antusias untuk melakukan inovasi pengembangan produk baru berbahan lokal berupa otak-otak ikan patin sebagai makanan jajanan sehat. Peningkatan pengetahuan mitra yaitu 85% dari awalnya kurang paham, menjadi sangat paham dan sudah dapat membuat otak-otak ikan patin dengan bentuk yang menarik, rasa yang enak dan kemasan yang bagus. Dengan pengembagan produk otak-otak ikan patin, diharapkan mitra dapat memperluas wilayah pemasaran, yang tidak hanya diwilayah sekitaran desa dan kabupaten tetapi bisa sampai tingkat provinsi bahkan ketingkat nasional. Sehingga dengan adanya inovasi pengembangan produk baru berupa otak-otak ikan dapat menambah varian kuliner baru pada mitra UMKM Ahmadi Kuliner, yang diharapkan nantinya dapat meningkatkan pendapatan ekonomi anggota mitra.