bluuebunny/crossref_metadata_2025_split huggingface dataset:
Excited State Behavior of Aromatic Amines in Solution: Dihydrophenazine Derivatives
Authors: Morris J. V. , Brühlmann U. , Serafimov O. , Huber J. Robert 
Published: October 2023

AbstractAbsorption and emission spectra as well as emission quantum yields and lifetimes of 5,10‐dimethylphenazine (DMP), 5‐methyl‐10‐phenylphenazine, and 5,10‐diphenylphenazine were measured in 3‐methylpentane at room temperature and 77°K. By means of relative polarization measurements in conjunction with CNDO/2‐CI calculations, the excited states were assigned. The long natural lifetime of the fluorescence (τf0 (DMP) ∼ 210 ns), together with the results of the calculation, reveals that the first transition is a symmetry forbidden 1A1 → 1A2 transition in a C2v geometry bent along the N‐N axis. This transition has an oscillator strength f0 ∼ 0.006 for all three compounds as determined from the fluorescence data. We propose that it acquires most of its intensity through a vibronic interaction with S2(1B1) (Herzberg‐Teller coupling). Since intersystem crossing between S1(1A2) and T1(3A2) is also orbitally forbidden, we suggest, in accordance with the phosphorescence polarization, an electronic spin‐orbit coupling between S2 and T1. Replacement of the methyl substituents by phenyl groups increases kisc and enhances the temperature dependent behavior of this parameter. Non‐radiative transitions occuring from the lowest triplet state account for nearly 40% of the absorbed quanta.
Collaborative technologies and project performance in manufacturing in the industry 4.0 environment: Mediating effect of individual, organizational, sociotechnical factors
Authors: Turi Jamshid Ali , Razzak Mohammad Rezaur , Bhuiyan Abul Bashar , Alam Mohammad Nurul , Al‐Kharusi Sami 
Published: October 2023

AbstractThe use of collaborative technologies (CT) in the industry 4.0 environment has become ubiquitous. While industry practitioners acknowledge the positive influence of CT on operations and projects of their organizations, yet there seem to be insufficient scholarly insights into the link between CT and project performance (PP). Hence, this study deploys the resource dependence theory to posit the influence of CT on PP, along with mediating roles of the individual factors (IF), organizational factors (OF), and sociotechnical factors (STF) in the above relationship. A set of hypotheses is developed and tested through cross‐sectional survey data collected from 392 employees in managerial and supervisory roles, in manufacturing organizations in the Kingdom of Saudi Arabia (KSA). The analysis conducted through structural equation modeling using SmartPLS indicates that CT positively influences PP. Moreover, the study found that IF, OF, and STF are all significant mediators in the relationships between CT and perceived project performance (PPP). This study contributes to the extant literature and industry practice by providing, nuanced insights into the role of pertinent mediators in the link between CT and PP.
Prediction algorithm for gastric cancer in a general population: A validation study
Authors: Wong Martin C. S. , Leung Eman Yee‐man , Yau Sarah T. Y. , Chan Sze Chai , Xie Shaohua , Xu Wanghong , Huang Junjie 
Published: October 2023

AbstractBackgroundWorldwide, gastric cancer is a leading cause of cancer incidence and mortality. This study aims to devise and validate a scoring system based on readily available clinical data to predict the risk of gastric cancer in a large Chinese population.MethodsWe included a total of 6,209,697 subjects aged between 18 and 70 years who have received upper digestive endoscopy in Hong Kong from 1997 to 2018. A binary logistic regression model was constructed to examine the predictors of gastric cancer in a derivation cohort (n = 4,347,224), followed by model evaluation in a validation cohort (n = 1,862,473). The algorithm's discriminatory ability was evaluated as the area under the curve (AUC) of the mathematically constructed receiver operating characteristic (ROC) curve.ResultsAge, male gender, history of Helicobacter pylori infection, use of proton pump inhibitors, non‐use of aspirin, non‐steroidal anti‐inflammatory drugs (NSAIDs), and statins were significantly associated with gastric cancer. A scoring of ≤8 was designated as “average risk (AR)”. Scores at 9 or above were assigned as “high risk (HR)”. The prevalence of gastric cancer was 1.81% and 0.096%, respectively, for the HR and LR groups. The AUC for the risk score in the validation cohort was 0.834, implying an excellent fit of the model.ConclusionsThis study has validated a simple, accurate, and easy‐to‐use scoring algorithm which has a high discriminatory capability to predict gastric cancer. The score could be adopted to risk stratify subjects suspected as having gastric cancer, thus allowing prioritized upper digestive tract investigation.
Dissection of MKK6 and p38 Signaling Using Light‐Activated Protein Kinases
Authors: Rahman Shah Md. Toufiqur , Zhou Wenyuan , Deiters Alexander , Haugh Jason M. 
Published: October 2023

AbstractStress‐activated signaling pathways orchestrate cellular behaviors and fates. Studying the precise role(s) of stress‐activated protein kinases is challenging, because stress conditions induce adaptation and impose selection pressure. To meet this challenge, we have applied an optogenetic system with a single plasmid to express light‐activated p38α or its upstream activator, MKK6, in conjunction with live‐cell fluorescence microscopy. In starved cells, decaging of constitutively active p38α or MKK6 by brief exposure to UV light elicits rapid p38‐mediated signaling, release of cytochrome c from mitochondria, and apoptosis with different kinetics. In parallel, light activation of p38α also suppresses autophagosome formation, similarly to stimulation with growth factors that activate PI3K/Akt/mTORC1 signaling. Active MKK6 negatively regulates serum‐induced ERK activity, which is p38‐independent as previously reported. Here, we reproduce that result with the one plasmid system and show that although decaging active p38α does not reduce basal ERK activity in our cells, it can block growth factor‐stimulated ERK signaling in serum‐starved cells. These results clarify the roles of MKK6 and p38α in dynamic signaling programs, which act in concert to actuate apoptotic death while suppressing cell survival mechanisms.
Silencing geranylgeranyltransferase I inhibits the migration and invasion of salivary adenoid cystic carcinoma through RhoA/ROCK1/MLC signaling and suppresses proliferation through cell cycle regulation
Authors: Chi Zengpeng , Wang Qimin , Tong Lei , Qiu Jing , Yang Fang , Guo Qingyuan , Li Wenjian , Zheng Jiawei , Chen Zhenggang 
Published: October 2023

AbstractGeranylgeranyltransferase type I (GGTase‐I) significantly affects Rho proteins, such that the malignant progression of several cancers may be induced. Nevertheless, the effect and underlying mechanism of GGTase‐I in the malignant progression of salivary adenoid cystic carcinoma (SACC) remain unclear. This study primarily aimed to investigate the role and mechanism of GGTase‐I in mediating the malignant progression of SACC. The level of GGTase‐I gene in cells was stably knocked down by short hairpin RNA‐EGFP‐lentivirus. The effects of GGTase‐I silencing on the migration, invasion, and spread of cells were examined, the messenger RNA levels of GGTase‐I and RhoA genes of SACC cells after GGTase‐I knockdown were determined, and the protein levels of RhoA and RhoA membrane of SACC cells were analyzed. Moreover, the potential underlying mechanism of silencing GGTase‐I on the above‐mentioned aspects in SACC cells was assessed by examining the protein expression of ROCK1, MLC, p‐MLC, E‐cadherin, Vimentin, MMP2, and MMP9. Furthermore, the underlying mechanism of SACC cells proliferation was investigated through the analysis of the expression of cyclinD1, MYC, E2F1, and p21CIP1/WAF1. Besides, the change of RhoA level in SACC tissues compared with normal paracancer tissues was demonstrated through quantitative reverse‐transcription polymerase chain reaction and western blot experiments. Next, the effect after GGTase‐I silencing was assessed through the subcutaneous tumorigenicity assay. As indicated by the result of this study, the silencing of GGTase‐I significantly reduced the malignant progression of tumors in vivo while decreasing the migration, invasion, and proliferation of SACC cells and RhoA membrane, Vimentin, ROCK1, p‐MLC, MMP2, MMP9, MYC, E2F1, and CyclinD1 expression. However, the protein expression of E‐cadherin and p21CIP1/WAF1 was notably upregulated. Subsequently, no significant transform of RhoA and MLC proteins was identified. Furthermore, RhoA expression in SACC tissues was significantly higher than that in paracancerous tissues. As revealed by the results of this study, GGTase‐I shows a correlation with the proliferation of SACC through the regulation of cell cycle and may take on vital significance in the migration and invasion of SACC by regulating RhoA/ROCK1/MLC signaling pathway. GGTase‐I is expected to serve as a novel exploration site of SACC.
Increased prescription dose for large vessel intravascular brachytherapy
Authors: Wallner Kent , Kearney Kathleen E. , Tiwana Jasleen , Pristera Nicole , Kim Edward Y. , Azzalini Lorenzo , Sandison George , Lombardi William L. , Don Creighton , Kim Minsun 
Published: October 2023

AbstractBackgroundMost randomized studies testing the effectiveness of IVBT were limited to vessels less than 4 mm diameter. In fact, it is now common to treat vessels larger than 4 mm. Accordingly, the authors instituted a prescription dose increase to 34 Gy at 2 mm from source center for vessels greater than 4.0 mm. The increase in prescription dose to 34 Gy at 2 mm from center is substantial, being 50% higher than the conventional maximum of 23 Gy.AimTo take a close look at group of patients treated to 34 Gy, and for whom follow‐up angiograms are available.MethodsTen patients treated for ISR with a prescription dose of 34 Gy and for whom follow‐up angiograms were available were studied. Beta‐radiation brachytherapy was performed with a Novoste Beta‐Cath System using a strontium‐90 (beta) source (Best Vascular, Springfield, VA). Source lengths of 40 or 60 mm were used. A dose of 34 Gy was prescribed at 2 mm from the source center.ResultsPatients were re‐catheterized from 2 to 21 months (median: 16 months) following IVBT, all for symptoms suggested of restenosis. All patients had some degree of ISR of the target vessel, but no IVBT‐treated vascular segment showed angiographic signs of degeneration, dissection or aneurysm.ConclusionThe authors' clinical impression, along with detailed review of the 10 cases, suggest that using a 34 Gy prescription dose at 2 mm from source center does not result in increased toxicity.
Successful closure of transcatheter aortic valve replacement‐induced Gerbode defect with valve‐in‐valve technique: A case report
Authors: Skalidis Ioannis , Hamilos Michalis , Petousis Stylianos , Kochiadakis George , Skalidis Emmanouil 
Published: October 2023

AbstractWe present the first documented case of a successful closure of a transcatheter aortic valve replacement (TAVR)‐induced Gerbode defect using a valve‐in‐valve approach. A 90‐year‐old female with severe aortic stenosis underwent TAVR. Following post‐dilatation, the patient experienced hemodynamic deterioration and collapse due to tamponade and sub‐annular rupture leading to hemodynamic deterioration and the development of a Gerbode defect with communication between the left ventricle and right atrium. Hemodynamic stabilization was achieved through pericardiocentesis, followed by the low implantation of a second valve, effectively sealing the rupture. This case showcases a valuable alternative for managing rare challenging complications during TAVR procedures.
Impact of prolonged drug‐coated balloon inflation on residual stenosis and clinical outcomes in coronary artery disease patients: A propensity score matched analysis
Authors: Ueda Hiroshi , Fujiwara Yoshihiro , Nishida Yusuke , Maenaka Motoyoshi , Yoshimura Kojiro , Oshida Yuki , Matsuhisa Seiji , Yoshida Naohiro , Yoshitani Hidetoshi , Kuga Yukiko , Ueda Kazuya , Nishida Yasunori 
Published: October 2023

AbstractBackgroundThere is a paucity of data regarding the optimal duration of drug‐coated balloon (DCB) inflation for coronary lesions. We sought to explore the effect of DCB angioplasty with versus without long inflation time on residual stenosis and clinical outcomes in patients with coronary artery disease.MethodsThis study included 314 consecutive patients with 445 lesions undergoing paclitaxel DCB angioplasty using different inflation time, divided according to whether the total inflation time of the DCB was ≥180 s (prolonged group) or <180 s (standard group). The primary clinical endpoint, defined as a composite of all‐cause death, myocardial infarction, stroke, or target lesion revascularization, was examined in 92 propensity score matched pairs.ResultsIn the matched cohort, the median clinical follow‐up period was 947 days. Postprocedural angiographic diameter stenosis was smaller in the prolonged group than in the standard group (30.0% [22.0–37.0] vs. 33.5% [25.5–40.5]; p = 0.042). Intravascular ultrasound measurements revealed that longer DCB inflation time resulted in smaller area stenosis (66.6 ± 7.8% vs. 69.4 ± 7.0%; p = 0.044) and a less mean increase in percent atheroma volume (−11.2 ± 7.1% vs. −7.4 ± 5.9%; p = 0.004) after angioplasty. The rate of the primary endpoint was lower in the prolonged group than in the standard group (log‐rank p = 0.025). The efficacy of prolonged DCB inflation was prominent in patients with in‐stent restenosis and longer lesions.ConclusionProlonged DCB inflation was associated with reduced residual stenosis and improved clinical outcomes in patients with coronary artery disease undergoing percutaneous coronary intervention. Prospective randomized trials are warranted to validate the benefits of DCB angioplasty with long inflation time.
Debulking the tricuspid valve with FlowTriever aspiration: A case series
Authors: Brown Brian 
Published: October 2023

AbstractIntracardiac masses adhering to the tricuspid valve can occur as a result of right‐sided infective endocarditis, malignancy, clot formation in the right atrium, or clots‐in‐transit passing through the right atrium. Early surgical intervention is recommended for tricuspid valve vegetation in some patients, although open heart surgery is not always an option. Treatment options for right heart thrombi include anticoagulation, thrombolysis, surgical embolectomy, or mechanical aspiration. We present a case series of tricuspid valve debulking using aspiration with the FlowTriever System.
Scalable Unseparated Bioelectrochemical Reactors by Using a Carbon Fiber Brush as Stirrer and Working Electrode
Authors: Hengsbach Jan‐Niklas , Engel Mareike , Cwienczek Marcel , Stiefelmaier Judith , Tippkötter Nils , Holtmann Dirk , Ulber Roland 
Published: October 2023

AbstractThe concept of energy conversion into platform chemicals using bioelectrochemical systems (BES) has gained increasing attention in recent years, as the technology simultaneously provides an opportunity for sustainable chemical production and tackles the challenge of Power‐to‐X technologies. There are many approaches to realize the industrial scale of BES. One concept is to equip standard bioreactors with static electrodes. However, large installations resulted in a negative influence on various reactor parameters. In this study, we present a new single‐chamber BES based on a stirred tank reactor in which the stirrer was replaced by a carbon fiber brush, performing the functions of the working electrode and the stirrer. The reactor is characterized in abiotic studies and electro‐fermentations with Clostridium acetobutylicum. Compared to standard reactors an increase in butanol production of 20.14±3.66 % shows that the new BES can be efficiently used for bioelectrochemical processes.
Generation of Bioactivity‐Tailored FK506/FK520 Analogs by CRISPR Editing in Streptomyces tsukubaensis
Authors: Buntin Kathrin , Mrak Peter , Pivk Lukančič Petra , Wollbrett Séverine , Drčar Tjasa , Krastel Philipp , Thibaut Christian , Salcius Michael , Gao Xiaolin , Wang Shaowen , Weber Eric , Koplan Eva , Regenass Hugo 
Published: October 2023

AbstractFor a potential application of FK506 in the treatment of acute kidney failure only the FKBP12 binding capability of the compound is required, while the immunosuppressive activity via calcineurin binding is considered as a likely risk to the patients. The methoxy groups at C13 and C15 are thought to have significant influence on the immunosuppressive activity of the molecule. Consequently, FK506 analogs with different functionalities at C13 and C15 were generated by targeted CRISPR editing of the AT domains in module 7 and 8 of the biosynthetic assembly line in Streptomyces tsukubaensis. In addition, the corresponding FK520 (C21 ethyl derivative of FK506) analogs could be obtained by media adjustments. The compounds were tested for their bioactivity in regards to FKBP12 binding, BMP potentiation and calcineurin sparing. 15‐desmethoxy FK506 was superior to the other tested analogs as it did not inhibit calcineurin but retained high potency towards FKBP12 binding and BMP potentiation.
Single Atom Stabilization of Phosphinate Ester‐Containing Rhodamines Yields Cell Permeable Probes for Turn‐On Photoacoustic Imaging**
Authors: Brøndsted Frederik , Fang Yuan , Li Lin , Zhou Xinqi , Grant Steven , Stains Cliff I. 
Published: October 2023

AbstractPhotoacoustic imaging (PAI) is an emerging imaging technique that uses pulsed laser excitation with near‐infrared (NIR) light to elicit local temperature increases through non‐radiative relaxation events, ultimately leading to the production of ultrasound waves. The classical xanthene dye scaffold has found numerous applications in fluorescence imaging, however, xanthenes are rarely utilized for PAI since they do not typically display NIR absorbance. Herein, we report the ability of Nebraska Red (NR) xanthene dyes to produce photoacoustic (PA) signal and provide a rational design approach to reduce the hydrolysis rate of ester containing dyes, affording cell permeable probes. To demonstrate the utility of this approach, we construct the first cell permeable rhodamine‐based, turn‐on PAI imaging probe for hypochlorous acid (HOCl) with maximal absorbance within the range of commercial PA instrumentation. This probe, termed SNR700‐HOCl, is capable of detecting exogenous HOCl in mice. This work provides a new set of rhodamine‐based PAI agents as well as a rational design approach to stabilize esterified versions of NR dyes with desirable properties for PAI. In the long term, the reagents described herein could be utilized to enable non‐invasive imaging of HOCl in disease‐relevant model systems.
Local rates of heat transfer and pressure losses in the vicinity of annular orifices
Authors: Williams P. S. , Knudsen J. G. 
Published: October 2023

AbstractA study was made of local rates of heat transfer and pressure losses in the vicinity of annular orifices. Various airflow rates and tube‐to‐orifice clearances were considered. Correlations were obtained for the value of the heat transfer coefficient at the orifice centre and the location and value of the maximum heat transfer coefficient downstream from the orifice. The marked effect of an upstream orifice on the coefficient at a downstream baffle is shown. Orifice pressure drop is correlated with a dimensionless pressure drop function, and results agree with those of previous workers.
Ultra‐wideband microwave imaging for breast tumor screening using step recovery diode‐based pulse generator
Authors: Liu Guancong , Xiao Xia , Lu Min , Zang Xuhui , Zhang Xiaofeng , Kikkawa Takamaro 
Published: October 2023

SummaryThe feasibility of a multistatic imaging system built on step recovery diode (SRD)‐based pulse generator for microwave breast cancer detection is investigated to develop a low‐cost and portable non‐invasive screening device in the future. The system consists of a pulse generator, an antenna array, and a sampling oscilloscope, in which the pulse generator generates monocycle pulse with a frequency bandwidth from 510 MHz to 6.1 GHz. Experiments are performed on a breast phantom made up of a breast model using 3D printing technology and soybean oil as internal filler, without the usage of any coupling medium. Four identical omnidirectional antennas sequentially transmit ultra‐wideband (UWB) signal and collect signals scattered inside the breast phantom, and then, the interior image of the phantom is reconstructed by background subtraction method and delay and sum (DAS) imaging algorithm. The system can successfully detect and distinguish a variety of test scenarios including cancerous breast phantom containing target with different size and different depth made by iron ball and bacon and healthy breast phantom. The smallest detectable target is 3 mm bacon, with signal to clutter ratio (SCR) and signal to mean ratio (SMR) metrics of 1.63 and 7.79 dB, respectively, and positioning error and relative error of 2.2 mm and 1.7%, respectively. It proves that the proposed time‐domain imaging system is promising for scaling up the detection range of early breast cancer.
Loss of species and genetic diversity during colonization: Insights from acanthocephalan parasites in northern European seals
Authors: Sromek Ludmila , Ylinen Eeva , Kunnasranta Mervi , Maduna Simo N. , Sinisalo Tuula , Michell Craig T. , Kovacs Kit M. , Lydersen Christian , Ieshko Evgeny , Andrievskaya Elena , Alexeev Vyacheslav , Leidenberger Sonja , Hagen Snorre B. , Nyman Tommi 
Published: October 2023

AbstractStudies on host–parasite systems that have experienced distributional shifts, range fragmentation, and population declines in the past can provide information regarding how parasite community richness and genetic diversity will change as a result of anthropogenic environmental changes in the future. Here, we studied how sequential postglacial colonization, shifts in habitat, and reduced host population sizes have influenced species richness and genetic diversity of Corynosoma (Acanthocephala: Polymorphidae) parasites in northern European marine, brackish, and freshwater seal populations. We collected Corynosoma population samples from Arctic, Baltic, Ladoga, and Saimaa ringed seal subspecies and Baltic gray seals, and then applied COI barcoding and triple‐enzyme restriction‐site associated DNA (3RAD) sequencing to delimit species, clarify their distributions and community structures, and elucidate patterns of intraspecific gene flow and genetic diversity. Our results showed that Corynosoma species diversity reflected host colonization histories and population sizes, with four species being present in the Arctic, three in the Baltic Sea, two in Lake Ladoga, and only one in Lake Saimaa. We found statistically significant population‐genetic differentiation within all three Corynosoma species that occur in more than one seal (sub)species. Genetic diversity tended to be high in Corynosoma populations originating from Arctic ringed seals and low in the landlocked populations. Our results indicate that acanthocephalan communities in landlocked seal populations are impoverished with respect to both species and intraspecific genetic diversity. Interestingly, the loss of genetic diversity within Corynosoma species seems to have been less drastic than in their seal hosts, possibly due to their large local effective population sizes resulting from high infection intensities and effective intra‐host population mixing. Our study highlights the utility of genomic methods in investigations of community composition and genetic diversity of understudied parasites.