bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of non-small cell lung cancer N2 metastasis using HIF-1α expression–related multicenter 18 F-FDG PET/CT radiomics
Authors: Ju Hyemin , Choi Joon Young , Kim Kangsan , Hwang Gil Tae , Woo Sang-Keun 
Published: November 2023

Abstract
The prognosis for stage III N2 metastatic non-small cell lung cancer (NSCLC) is poor. We aimed to develop an N2 metastasis prediction machine learning model using multicenter 18F- fluorodeoxyglucose (FDG) positron emission tomography (PET) radiomics correlating with hypoxia-inducible factor (HIF)-1α expression levels. Internal and external cohorts consisted of data of 66 patients from public databases and 102 patients from external institutions. Hub genes associated with metastasis were identified via functional enrichment analysis using stage III N2 metastasis and stage Ⅰ non-metastasis patients. The data of patients with pathological stage T2a or higher (tumor size > 3 cm) were extracted. Features were calculated from 18F-FDG PET/computed tomography (CT) images; internal and external cohorts were harmonized using the ComBat algorithm. Image features of the prediction model were selected based on the area under the receiver operating characteristic curve (AUC). Using functional gene analysis, HIF-1α was confirmed to be associated with N2 metastasis. Either radiomics correlated with hub genes or HIF-1α gene expression levels were used to construct RF models. The prediction performance of the model was the highest when using image features correlated with HIF-1α expression (accuracy = 0.83, AUC = 0.819). The RF model utilizing harmonized image features showed high performance for the cohort with high tumor size. Harmonization of radiomics is required when developing a machine learning model for predicting N2 metastasis using multicenter data. As lymph node involvement is the main prognostic factor, the prediction of N2 metastasis could facilitate personalized therapeutic strategies for NSCLC.
Chromosome-level genome assembly of Oncomelania hupensis: the intermediate snail host of Schistosoma japonicum
Authors: Liu Qin , Duan Lei , Guo Yun-Hai , Yang Li-Min , Zhang Yi , Li Shi-Zhu , Lv Shan , Hu Wei , Chen Nan-Sheng , Zhou Xiao-Nong 
Published: November 2023

Abstract
Background
Schistosomiasis is a zoonotic parasitic disease that is estimated to affect almost 300 million people worldwide. As one of the three major human parasites, Schistosoma japonicum is endemic in Southeast Asia, including China, Philippines and Indonesia. Transmission relies only on one snail host Oncomelania hupensis (Gastropoda: Pomatiopsidae). However, no genomic information for this important intermediate host is available.
Methods
Contig-level of O. hupensis assembly of an individual male O. hupensis snail was performed with full PacBio long reads using Next Denovo. The completeness and continuity of the assembly were assessed with Benchmarking Universal Single-Copy Orthologs (BUSCO). CAFÉ was used to do analysis of contraction and expansion of the Gene family and CodeML module in PAML was used for positive selection analysis in protein coding sequences.
Results
A total length of 1.46 Gb high-quality O. hupensis genome with 17 unique full-length chromosomes (2n = 34) of the individual including a contig N50 of 1.35 Mb and a scaffold N50 of 75.08 Mb. Additionally, 95.03% of these contig sequences were anchored in 17 chromosomes. After scanning the assembled genome, a total of 30,604 protein-coding genes were predicted. Among them, 86.67% were functionally annotated. Further phylogenetic analysis using single copy protein-coding genes revealed that O. hupensis was separated from a common ancestors of Pomacea canaliculata and Bellamya purificata approximately 170 million years ago. Comparing the genome of O. hupensis with its most recent common ancestor, it showed 266 significantly expanded gene families (p < 0.05) and 58 significantly contracted gene families (p < 0.05). Functional enrichment of the expanded gene families indicated that they were mainly involved with intracellular, DNA-mediated transposition, DNA integration, transposase activity and hyalurononglucosaminidase activity. Additionally, a total of 281 protein-coding genes related to protein kinase activity, protein phosphorylation, catalytic activity and metabolic process under positive selection were identified in O. hupensis (FDR < 0.05).
Conclusion
A complete genome sequence of O. hupensis would enable the fundamental understanding of snail biology as well as its co-evolution with the S. japonicum parasite causing schistosomiasis in humans. Using a single molecular DNA sequencing technology, we have successfully constructed the genome at the chromosomal-level. These data will not only provide the compressive genomic information for the sole intermediated host of S. japonicum, but also benefit future work on population genetics of this snail as well as evolutional studies between S. japonicum and the snail host.
Imprints of Sagittarius accretion event: Young O-rich stars
and discontinuous chemical evolution in Milky Way disc
Authors: Bi Shao-Lan , Sun Tiancheng , Chen Xunzhou , Chen Yuqin , Liu Chao , Zhang Xianfei , Li Tanda , Li Yaguang , Wu Ya-Qian , Ge Zhishuai , Ye Lifei 
Published: November 2023

Abstract

The Milky Way has undergone significant transformations in its early history, characterised by violent mergers and the accretion of satellite galaxies. Among these events, the infall of the satellite galaxy Gaia-Enceladus/Sausage is recognised as the last major merger event, fundamentally altering the evolution of the Milky Way and shaping its chemo-dynamical structure. However, recent observational evidence suggests that the Milky Way remains undergone notable events of star formation in the past 4 Gyr, which is thought to be triggered by the perturbations from Sagittarius dwarf galaxy (Sgr). Here we report chemical signatures of the Sgr accretion event in the past 4 Gyr, using the [Fe/H] and [O/Fe] ratios in the thin disc, which is reported for the first time. It reveals that the previously discovered V-shape structure of age-[Fe/H] relation varies across different Galactic locations and has rich substructures. Interestingly, we discovery a discontinuous structure at zmax < 0.3 kpc, interrupted by a recent burst of star formation from 4 Gyr to 2 Gyr ago. In this episode, we find a significant rise in oxygen abundance leading to a distinct [O/Fe] gradient, contributing to the formation of young O-rich stars. Combined with the simulated star formation history and chemical abundance of Sgr, we suggest that the Sgr is an important actor in the discontinuous chemical evolution of the Milky Way disc.
Batch Electrocoagulation Process for the Removal of High Colloidal Clay from Open-Cast Coal Mine Water using Al and Fe Electrodes
Authors: Abfertiawan Muhammad Sonny , Syafila Mindryani , Handajani Marisa , Hasan Faiz , Oktaviani Hanifah , Gunawan Firman , Djali Febriwiadi 
Published: November 2023

Abstract

Open-cast coal mining, which involves massive excavation and stockpiling of overburden, has the potential to affect surface water quality. In addition to the potential generation of acid mine drainage owing to the presence of sulfide minerals, mining operations also have the potential to produce water with high total suspended solids (TSS) concentrations. This can occur owing to the presence of clay materials, especially colloidal clays, which prevent solid particles from settling naturally. This study aims to develop an alternative electrocoagulation technology that can be used to remove colloidal clay from mine water. Monopolar batch electrocoagulation was performed at a laboratory scale using aluminum and iron electrodes with variations in the current (0.5, 1, and 2 A) and contact time (15, 30, and 45 min). The results showed that aluminum electrode electrocoagulation, with a current of 2 A and a contact time of 15 min, had a TSS removal efficiency of 99.58%, with concentrations decreasing from 5,400 to 22.84 mg/L. Iron electrode electrocoagulation achieved the highest efficiency with a current of 2 A and a contact time of 30 min. The TSS removal efficiency reached 98.78% while the concentration dropped to 65.66 mg/L. This study is the initial stage to develop electrocoagulation technology. This technology may become an essential alternative for treating mine water with colloidal clay in the future.
Segmenting tourism companies with relational and technological bases
Authors: Fuentes-Blasco Maria , Moliner-Velázquez Beatriz , Gil-Saura Irene , Berenguer-Contrí Gloria 
Published: November 2023

Abstract

The objective of this study is to achieve a travel agencies segmentation based on both relational (trust, commitment, satisfaction, and loyalty) and technological (advancement and use of Information and Communication Technologies) criteria that improve the understanding of their strategic behaviours. The segmentation methodology uses a tandem approach: correspondence and hierarchical cluster analysis. From a sample of 256 travel agencies, four segments have been identified. Relational criteria have made it possible to segment only the retail agency market, while technological criteria have been shown to be more capable of segmenting the wholesale agency market. This work contributes to the advancement of the literature on business-to-business segmentation in tourism by providing a more complete vision of the segmentation of companies. From a practical approach, it allows a better knowledge of the agency segments, so it could be used for the selection not only of providers (customer perspective) but also of target segments (service provider perspective).
Allosteric Neutralization by Human H7N9 Antibodies
Authors: Wu Xueling , Jia Manxue , Zhao HanJun , Morano Nicholas , Lu Hong , Lui Yin-Ming , Du Haijuan , Becker Jordan , Yuen Kwok-Yung , Ho David , Kwong Peter , Shapiro Lawrence , To Kelvin Kai-Wang 
Published: November 2023

Abstract

The avian influenza A virus H7N9 causes severe human infections with more than 30% fatality despite the use of neuraminidase inhibitors. Currently there is no H7N9-specific prevention or treatment for humans. From a 2013 H7N9 convalescent case occurred in Hong Kong, we isolated four H7 hemagglutinin (HA)-reactive monoclonal antibodies (mAbs) by single B cell cloning, with three mAbs directed to the HA globular head domain (HA1) and one to the HA stem region (HA2). Two clonally related HA1-directed mAbs, H7.HK1 and H7.HK2, potently neutralized H7N9 and protected mice from a lethal H7N9/AH1 challenge. Cryo-EM structures revealed that H7.HK1 and H7.HK2 bind to a β14-centered surface partially overlapping with the antigenic site D of HA1 and disrupt the 220-loop that makes hydrophobic contacts with sialic acid on the adjacent protomer, thus affectively blocking viral entry. The more potent mAb H7.HK2 retained full HA1 binding and neutralization capacity to later H7N9 isolates from 2016-2017, which is consistent with structural data showing that the antigenic mutations of 2016-2017 from the 2013 H7N9 only occurred at the periphery of the mAb epitope. The HA2-directed mAb H7.HK4 lacked neutralizing activity but protected mice from the lethal H7N9/AH1 challenge when engineered to mouse IgG2a enabling Fc effector function in mice. Used in combination with H7.HK2 at a suboptimal dose, H7.HK4 augmented mouse protection. Our data demonstrated an allosteric mechanism of mAb neutralization and augmented protection against H7N9 when a HA1-directed neutralizing mAb and a HA2-directed non-neutralizing mAb were combined.
Hyaluronan in mesenchymal stromal cell lineage differentiation from human pluripotent stem cells: Application in serum free culture
Authors: De Sousa Paul A , Perfect Leo , Ye Jinpei , Samuels Kay , Piotrowska Ewa , Gordon Martin , Abranches Elsa , Wishart Thomas M , Dockrell David H , Courtney Aidan , Mate Ryan 
Published: November 2023

Abstract

Background
Hyaluronan (HA) is an extracellular glycosaminoglycan polysaccharide with widespread roles throughout development and in healthy and neoplastic tissues. In pluripotent stem cell culture it can support both stem cell renewal and differentiation. However, responses to HA in culture are influenced by interaction with a range of cognate factors and receptors including components of blood serum supplements, which alter results. These may contribute to variation in cell batch production yield and phenotype as well as heighten the risks of adventitious pathogen transmission in the course of cell processing for therapeutic applications.
Main
Here we characterise differentiation of a human embryo/pluripotent stem cell derived Mesenchymal Stromal Cell (hESC/PSC-MSC)-like cell population by culture on a planar surface coated with HA in serum-free media qualified for cell production for therapy. Resulting cells met minimum criteria of the International Society for Cellular Therapy for identification as MSC by expression of. CD90, CD73, CD105, and lack of expression for CD34, CD45, CD14 and HLA-II. They were positive for other MSC associated markers (ie.CD166, CD56, CD44, HLA 1-A) whilst negative for others (eg. CD271, CD71, CD146). In vitro co-culture assessment of MSC associated functionality confirmed support of growth of hematopoietic progenitors and inhibition of mitogen activated proliferation of lymphocytes from umbilical cord and adult peripheral blood mononuclear cells, respectively. Co-culture with immortalized THP1 monocyte derived macrophages (Mɸ) concurrently stimulated with lipopolysaccharide as a pro-inflammatory stimulus, resulted in a dose dependent increase in pro-inflammatory IL6 but negligible effect on TNFα. To further investigate these functionalities, a bulk cell RNA sequence comparison with adult human bone marrow (hBM) derived MSC and hESC substantiated a distinctive genetic signature more proximate to the former.
Conclusion
Cultivation of human pluripotent stem cells on a planar substrate of HA in serum-free culture media systems is sufficient to yield a distinctive mesenchymal stromal cell lineage with prospective therapeutic utilities for tissue morphogenesis and repair.
Diagnosis Challenges when Substance Use and Trauma are Part of the Narrative: A Review of Human Trafficking Cases Among Women and Critical Points of Learning for the Mental Health Community
Authors: Lavy-Joy Nicole Lavy-Joy 
Published: November 2023

Abstract
Human trafficking survivors often have a myriad of complex mental health issues because of their experiences. Research has shown there is a high correlation between substance use and post-traumatic stress disorder among survivors. The mental health challenges can become more complex when trauma and/or substance use is misdiagnosed or underdiagnosed. The results of this study show the prevalence of both situations among a program serving adult female survivors from within the US. The study documents the more common inaccurate diagnoses for this population of women, and the change in diagnosis after a dedicated period of assessment was completed. The discussion includes lack of understanding of how trauma and substance use present in a clinical or medical setting, an overfocus on other symptoms, lack of education and training among law enforcement, courts, and community service providers, and the exacerbated problems for survivors because of the incorrect or inaccurate diagnoses.
Urbanization and Wetland Degradation: Land Use Land Cover Change Analysis of the Aberdeen Creek, Sierra Leone River Estuary
Authors: Suleiman Aloysious Kingsley , Yang Changming , Sesay Alpha 
Published: November 2023

Abstract
The rapid urbanization of the global population is having devastating impacts on wetlands globally. In Sierra Leone, urbanization has resulted in the degradation of mangroves and the overall destruction of the wetland around Aberdeen Creek. This study therefore maps out, characterizes, and assesses the Land Use Land Cover Change, and potentials of Aberdeen Creek situated on the Sierra Leone River Estuary. The study will serve as an eye-opener for sustainable land use planning and preserving the Wetland under the supervision of the Ramsar Convention. In that light, the research was carried out using remote sensing and GIS techniques to analyze the extent of degradation on the wetland in three time series, which were mainly driven by Landsat imageries acquired in 2000, 2023 and predicted a 2033 scenario if urgent actions are not taken. The results show that there is an exponential settlement and infrastructural growth in the study area resulting to the quest for land, housing and other facilities/infrastructures that have given rise to changes in land cover, reduction and loss of the Wetlands. Furthermore, comparing the various time series, the result shows that, by the year 2000 there were abundant mangroves, but over two decades later in 2023, there is a drastic reduction in the mangroves, and the 2033 estimation predicted a worrisome outcome for the wetland. The study equally revealed alarming rates of environmental degradation due to ineffective legal frameworks and enforcement systems. It is therefore recommended that urgent actions be taken to evacuate people from those areas, halt further encroachment and regenerate lost species and ecosystems.
Gas Reservoir Detection Using Mixed Components Short Time Fourier Transform (MC-STFT) as a new attribute
Authors: Jalali Ali , Bagheri Majid 
Published: November 2023

Abstract
Identification of gas reservoirs as a main natural resource due to its economic importance has always been one of the most important issues in research studies in the oil and gas field. Accurate localization of a gas reservoir through seismic data has been broadly studied. The final destination of all seismic attributes is to distinguish a specific feature and make it detectable. Accordingly, many seismic attributes have been developed among which short time Fourier transform (STFT)-based methods play an important role. However, simplicity and efficiency can make a method superior. So we propose an attribute which utilizes mixed components of STFT (MC-STFT). The novelty about this method is that without altering STFT method or adding any complexity, MC-STFT is able to detect gas reservoirs at high resolution. In fact, this method takes advantage of extracting three frequency components obtained by STFT. We apply this method on three data sets, first, Marmousi model and then two other real seismic zero-offset sections. Numerical results confirm its good performance in high resolution gas reservoir detection.
Revealing the Pattern of Causality in Tourist Experience and Perception of Sacredness in Shamanic Heritage Destinations: Evidence from Northeast China
Authors: Lin Chunhua , Wang Guanqiang , Zeng Bing-Yu , Li Si-Jie , Xiong Lei , Yang Hua 
Published: November 2023

Abstract

With the growth of cultural route tourism and the transformation and upgrading of the needs and preferences of major consumer groups, maintaining and highlighting the sanctity of religious heritage tourism destinations is one of the key factors affecting the appeal of cultural tourism. The purpose of this study is to clarify the causal association rules between tourists’ tourism experience characteristics/attributes and destination sacredness perceptions, and then explore resource development and place-making strategies in religious tourism destinations with the goal of continuously enhancing local sacredness. This study collected 374 tourist questionnaire data, and applied rough set theory (RSA) and decision-making laboratory analysis method (DEMATEL) to conduct data exploration and analysis. The results of this study highlight the impact of architectural and landscape features of shamanic religious tourism destinations on tourists’ perceptions of sacredness. In addition, tourists' interpretation and participation are also important condition attributes. The results of this study will provide a key theoretical foundation for subsequent research. For the empirical cases in this study, this study provides a key decision-making reference for local culture and tourism management departments to enhance the destination's cultural tourism attraction and sustainability.
Inhibition of PbeXTH1 and PbeSEOR1 is required for the Valsa canker resistance contributed by Wall-associated kinase gene MbWAK1
Authors: Lu Yuan , Mao Xia , Wang Chao , Zheng Yan , Duo Hu , Sun E , Yu Hongqiang , Chen Zhongjian , ZUO Cunwu 
Published: November 2023

Abstract
Wall-associated kinases (WAKs) were determined the role for perceiving pathogenic signals and initiation of plant immune responses. However, the roles of the family members in host resistance against Valsa canker, a serious fungal disease of apple and pear, are largely unknown. Here, we identified MbWAK1in Malus baccata, a resistant germplasm, was differentially expressed during infection by Valsa mali (Vm) and multiple stress-related signals. Over-expression MbWAK1 enhanced the Valsa canker resistance of apple and pear fruits and ‘Duli-G03’ (Pyrus betulifolia) suspension cells. A large number of phloem, cell wall and lipid metabolic process-related genes were differentially expressed during overexpressed suspension cells lines respond to Valsa pyri (Vp) signals. Among these, the expression of xyloglucan endotransglucosylase/hydrolase gene PbeXTH1 and sieve element occlusion-related gene PbeSEOR1 were significantly inhibited. Transient expression of PbeXTH1 or PbeSEOR1 obviously compromised the expressional induction of MbWAK1 and the resistance contributed by MbWAK1. In addition, MbWAK1 interacted with PbeXTH1 and PbeSEOR1, as well as several patterns recognize receptors, such as MdBAK1 and MdCERK1. Our results enriched the molecular mechanisms for MbWAK1 against Valsa canker and resistant breeding.
The dominant role of bacterial community and network characteristics drive the humification process and greenhouse gas emissions during plant residues composting under different aeration rates
Authors: Gu Junyu , CAO YUN , Sun Qian , Xu YUEDING , Huang Hongying 
Published: November 2023

Abstract
The study investigated the effects of different aeration intensities on organic matter (OM) degradation, greenhouse gas emissions (GHG) as well as humification during plant residue composting. Three intermittent aeration intensities of 0.16 (T1), 0.38 (T2) and 0.68 (T3) L/kg DM/min with 30 min on/30 min off were conducted on a lab-scale composting experiment. Results showed that OM mineralization in T3 was more evident than T1 and T2, resulting in the highest humification degree and humic acids (HA) content. HA content in T2 and T3 was 7.68% and 10.28% higher than that in T1. Medium aeration intensity was more favorable for emission of total GHG emission. Linear Discriminant Analysis Effect Size analysis showed that the biomarkers within T1 mainly belonged to Anaerolineaceae, while Thermostaphylospora was identified as biomarkers in T2. The enrichment of thermophilic bacterial genera may play important roles on promoting humification in T2 and T3. Bacterial network analysis showed that T3 had the most key nodes and edges. The interaction between bacterial community was strengthened and the network relationship was closest in T3. The Structural Equation Model confirmed the roles of dominant bacterial community on promoting humification. Oxygen concentration and pH indirectly affected both the mineralization and humification through the action of key microorganisms.