bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mosaic sarbecovirus nanoparticles elicit cross-reactive responses in pre-vaccinated animals
Authors: Cohen Alexander A. , Keeffe Jennifer R. , Schiepers Ariën , Dross Sandra E. , Greaney Allison J. , Rorick Annie V. , Gao Han , Gnanapragasam Priyanthi N.P. , Fan Chengcheng , West Anthony P. , Ramsingh Arlene I. , Erasmus Jesse H. , Pata Janice D. , Muramatsu Hiromi , Pardi Norbert , Lin Paulo J.C. , Baxter Scott , Cruz Rita , Quintanar-Audelo Martina , Robb Ellis , Serrano-Amatriain Cristina , Magneschi Leonardo , Fotheringham Ian G. , Fuller Deborah H. , Victora Gabriel D. , Bjorkman Pamela J. 
Published: February 2024

SUMMARYImmunization with mosaic-8b [60-mer nanoparticles presenting 8 SARS-like betacoronavirus (sarbecovirus) receptor-binding domains (RBDs)] elicits more broadly cross-reactive antibodies than homotypic SARS-CoV-2 RBD-only nanoparticles and protects against sarbecoviruses. To investigate original antigenic sin (OAS) effects on mosaic-8b efficacy, we evaluated effects of prior COVID-19 vaccinations in non-human primates and mice on anti-sarbecovirus responses elicited by mosaic-8b, admix-8b (8 homotypics), or homotypic SARS-CoV-2 immunizations, finding greatest cross-reactivity for mosaic-8b. As demonstrated by molecular fate-mapping in which antibodies from specific cohorts of B cells are differentially detected, B cells primed by WA1 spike mRNA-LNP dominated antibody responses after RBD-nanoparticle boosting. While mosaic-8b- and homotypic-nanoparticles boosted cross-reactive antibodies, de novo antibodies were predominantly induced by mosaic-8b, and these were specific for variant RBDs with increased identity to RBDs on mosaic-8b. These results inform OAS mechanisms and support using mosaic-8b to protect COVID-19 vaccinated/infected humans against as-yet-unknown SARS-CoV-2 variants and animal sarbecoviruses with human spillover potential.
Geographically widespread and novel hemotropic mycoplasmas and bartonellae in Mexican free-tailed bats and sympatric North American bat species
Authors: Becker Daniel J. , Dyer Kristin E. , Lock Lauren R. , Olbrys Beckett L. , Pladas Shawn A. , Sukhadia Anushka A. , Demory Bret , Nunes Batista Juliana Maria , Pineda Micaela , Simmons Nancy B. , Adams Amanda M. , Frick Winifred F. , O’Mara M. Teague , Volokhov Dmitriy V. 
Published: February 2024

AbstractBacterial pathogens remain poorly characterized in bats, especially in North America. We describe novel (and in some cases panmictic) hemoplasmas (10.5% positivity) and bartonellae (25.5% positivity) across three colonies of Mexican free-tailed bats (Tadarida brasiliensis), a partially migratory species that can seasonally travel hundreds of kilometers. Molecular analyses identified three novelCandidatushemoplasma species most similar to another novelCandidatusspecies in Neotropical molossid bats. We also detected novel hemoplasmas in sympatric cave myotis (Myotis velifer) and pallid bats (Antrozous pallidus), with sequences in the latter 96.5% related toC. Mycoplasma haemohominis. We identified nineBartonellagenogroups, including those in cave myotis with 96.7% similarity toC. Bartonella mayotimonensis. We also detectedBartonella rochalimaein migratory Mexican free-tailed bats, representing the first report of this human pathogen in the Chiroptera. The seasonality and diversity of these bacteria observed here suggest that additional longitudinal, genomic, and immunological studies in bats are warranted.
A single giant cell at the origin of an evolutionary innovation
Authors: Badiane Arnaud , Viala Séverine , Armisen David , Mignerey Ilona , Forcet-Vauchel Christelle , De Jesús Villanueva Christina N. , Van Belleghem Steven , Crumière Antonin , Santos Emilia , Francesconi Mirko , Khila Abderrahman 
Published: February 2024

AbstractKey innovations play a central role in the diversification of lineages, yet our understanding of the mechanisms underlying their emergence remains fragmented. The propelling fan is a key innovation associated with the diversification ofRhagoveliawater striders into fast flowing streams. Here, we show that a single giant cell is responsible for the development of the fan throughout embryogenesis. RNA interference against the fan-specific gene familygsha/mogshadepleted the fan in the embryo but did not alter the giant cell, indicating that these genes are not required for specifying the identity of this cell.gsha/mogshainstead seem to activate the expression of cuticular genes during early development, suggesting a role of these genes in regulating the accumulation of fan structural proteins. We also show that Hox genes act to block the giant cell in the fore- and rear-legs, thus restricting its fate to the mid-legs – the pair of legs that generates movement on water. In addition, Hox expression is specifically excluded from the giant cell, thus allowing the expression of the fan’s developmental genetic program. This study reveals that a single cell can be sufficient to generate innovative traits that have been central for a lineage to acquire new ecological opportunities and burst into diversification.One-sentence summaryA single giant cell orchestrates the developmental genetic program of a key innovation
Proteomics analysis reveals novel phosphorylated residues and associated proteins of the polyomavirus DNA replication initiation complex
Authors: Dey-Rao Rama , Shen Shichen , Qu Jun , Melendy Thomas 
Published: February 2024

AbstractPolyomavirus (PyV) Large T-antigen (LT) is the major viral regulatory protein that targets numerous cellular factors/pathways: tumor suppressors, cell cycle regulators, transcription and chromatin regulators, as well as other factors for viral replication. LT directly recruits the cellular replication factors involved in LT’s recognition of the viral origin, origin unwinding, and primer synthesis which is carried out by mutual interactions between LT, DNA polymerase alpha-primase (Polprim), and single strand (ss) DNA binding replication protein A (RPA). The activities as well as interactions of these three with each other as well as other factors, are known to be modulated by post-translational modifications (PTMs); however, modern high-sensitivity proteomic analyses of the PTMs as well as proteins associated with the three have been lacking. Elution from immunoprecipitation (IP) of the three factors were subjected to high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). We identified 479 novel phosphorylated amino acid residues (PAARs) on the three factors: 82 PAARs on SV40 LT, 305 on the Polprim heterotetrametric complex and 92 on the RPA heterotrimeric complex. LC-MS/MS analysis also identified proteins that co-immunoprecipitated (coIP-ed) with the three factors that were not previously reported: 374 with LT, 453 with Polprim and 183 with RPA. We used a bioinformatic-based approach to analyze the proteomics data and demonstrate a highly significant “enrichment” of transcription-related process associated uniquely with LT, consistent with its role as a transcriptional regulator, as opposed to Polprim and RPA associated proteins which showed no such enrichment. The most significant cell cycle related network was regulated by ETS proto-oncogene 1 (ETS1), indicating its involvement in regulatory control of DNA replication, repair, and metabolism. The interaction between LT and ETS1 is validated and shown to be independent of nucleic acids. One of the novel phosphorylated aa residues detected on LT from this study, has been demonstrated by us to affect DNA replication activities of SV40 Large T-antigen. Our data provide substantial additional novel information on PAARs, and proteins associated with PyV LT, and the cellular Polprim-, RPA- complexes which will benefit research in DNA replication, transformation, transcription, and other viral and host cellular processes.
Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets
Authors: Lee Jeong Min , Zeng Jing , Liu Pengpeng , Nguyen My Anh , Loustaunau Diego Suchenski , Bauer Daniel E. , Yilmaz Nese Kurt , Wolfe Scot A. , Schiffer Celia A. 
Published: February 2024

AbstractOver the last 5 years, cytosine base editors (CBEs) have emerged as a promising therapeutic tool for specific editing of single nucleotide variants and disrupting specific genes associated with disease. Despite this promise, the currently available CBE’s have the significant liabilities of off-target and bystander editing activities, in part due to the mechanism by which they are delivered, causing limitations in their potential applications. In this study we engineeredhighly stabilized Cas-embedded CBEs (sCE_CBEs) that integrate several recent advances, andthat are highly expressible and soluble for direct delivery into cells as ribonucleoprotein (RNP) complexes. Our resulting sCE_CBE RNP complexes efficiently and specifically target TC dinucleotides with minimal off-target or bystander mutations. Additional uracil glycosylase inhibitor (UGI) protein intransfurther increased C-to-T editing efficiency and target purity in a dose-dependent manner, minimizing indel formation to untreated levels. A single electroporation was sufficient to effectively edit the therapeutically relevant locus for sickle cell disease in hematopoietic stem and progenitor cells (HSPC) in a dose dependent manner without cellular toxicity. Significantly, these sCE_CBE RNPs permitted for the transplantation of edited HSPCs confirming highly efficient editing in engrafting hematopoietic stem cells in mice. The success of the designed sCBE editors, with improved solubility and enhanced on-target editing, demonstrates promising agents for cytosine base editing at other disease-related sites in HSPCs and other cell types.
Freeze-in bino dark matter in high-scale supersymmetry
Authors: Han Chengcheng , Wu Peiwen , Yang Jin Min , Zhang Mengchao 
Published: February 2024

We explore a scenario of high-scale supersymmetry where all supersymmetric particles except gauginos stay at a high-energy scale MSUSY that is much larger than the reheating temperature TRH. The dark matter is dominated by a bino component with mass around the electroweak scale and the observed relic abundance is mainly generated by the freeze-in process during the early Universe. Considering the various constraints, we identify two available scenarios in which the supersymmetric sector at an energy scale below TRH consists of (a) bino or (b) bino and wino. Typically, for a bino mass around 0.1–1 TeV and a wino mass around 2 TeV, we find that MSUSY should be around 1013−14  GeV with TRH around 104−6  GeV.

Published by the American Physical Society
2024
Digital enabled innovation ecosystems: a dual case study of knowledge flows in intellectual property platforms
Authors: Ma Lei , Zhang Ben , Liang Kaitong , Cheng Yang , Yi Chaonan 
Published: February 2024

PurposeThe embedding of digital technology and the fuzzy organizational boundary have changed the operation of platform innovation ecosystem (PIE). Specifically, as an important energy of PIE, the internal logic of knowledge flow needs to be reconsidered in the context of digital age, which will be helpful to select the cultivation and governance strategy of PIE.Design/methodology/approachA dual case-analysis is applied to open the “black box” of knowledge flow in the PIE from the perspective of enabled by digital technology, by taking the intellectual property (IP) operation platform as cases.FindingsThe research findings are as follow: (1) The knowledge flow mechanism of PIE is mainly demonstrated through the processes of knowledge acquisition, knowledge integration and knowledge spillover. During this process, connectivity empowerment and scenario empowerment realize the digital empowerment of the platform. (2) Connectivity empowerment provides a channel of knowledge acquisition for the digital connection between participants in PIE. In the process of knowledge integration, scenario empowerment improves the opportunities for accurate matching and collaborative innovation between knowledge supplier and demander, and enhance the value of knowledge. The dual effect of connectivity empowerment and scenario empowerment has accelerated the knowledge spillover in PIE. Particularly, connectivity empowerment expands the range of knowledge spillover, and scenario empowerment affects the generativity of the platform, resulting in the enhancement of platform’s capability to embed and expand its value network. (3) Participants have been benefitted from the PIE enabled by digital technology through three key modules (knowledge acquisition, knowledge integration and knowledge spillover), as the result of knowledge flow.Originality/valueThis study focuses on the knowledge flow mechanism of PIE enabled by digital technology, which enriches the PIE theory, and has enlightenments for the cultivation of digital platform ecosystem.
A comparative study on sustainability standards with specific reference to GRI standards and BRSR framework
Authors: Nial Naresh , Parashar Pranay 
Published: February 2024

PurposeThe main objective of the study was to compare Business Responsibility and Sustainability Report (BRSR) norms with Global Reporting Initiative (GRI) standards, so as to establish whether BRSR norms match the global standards and best practices or not. Additionally, an effort was made to ascertain and highlight areas where BRSR norms are more comprehensive, just match, or require further refinement to be at par with the GRI standards. The study highlights the similarities and dissimilarities between the internationally accepted GRI standards and the BRSR framework; thereby suggesting areas of improvement for the BRSR framework.Design/methodology/approachScrutinised all the 36 standards of the Global reporting initiative and BRSR format and guidelines of the Securities Exchange Board of India. The Content Analysis Technique was used to ascertain the percentages of similarities between the two frameworks.FindingsThe content analysis found that there are 52.30% similarities between BRSR norms and GRI standards. Further, this study shows the factors that led to the dissimilarities between BRSR and GRI standards. This study found 18 areas where BRSR is more informative than GRI, and 7 areas where BRSR could be further refined.Originality/valueThis study contributes to research in the sustainability reporting framework to be adopted by Indian listed companies. There are a few Indian listed companies who are already reporting as per the GRI framework and might perceive the BRSR as a separate reporting altogether. But as found in this study, more than half of the BRSR framework is similar to the GRI framework; thus, half the work is almost done. As such this study helps Indian firms in developing an understanding of the BRSR and puts in perspective its standing among global sustainability reporting standards. This study shall help institutional investors, rating agencies, and external assurers to better visualize an Indian entity, by referring to its Business Responsibility and Sustainability Reporting.
Weaving emotions: unraveling the emotional significance of Sri Lankan handloom artistry
Authors: Gopura Sumith , Wickramasinghe Ayesha 
Published: February 2024

PurposeThis paper examines the socio-emotional identities of handloom artisans in Sri Lanka as a novel technique for new product development.Design/methodology/approachQualitative research methods, including observational research and semi-structured interviews with 27 artisans from different handloom communities in Sri Lanka were conducted and analyzed in thematic approach.FindingsBy highlighting the maker of the craft through their socio-emotional identities in an artisan-oriented approach, this paper provides insight into new product development for handloom in alignment with up-and-coming trends. Ultimately, this can increase the demand for handloom and sustain the sector in both local and international fashion markets.Originality/valueThis research study is one of the first of its kind to propose a novel approach for artisan-oriented product development through the application of artisans’ socio-emotional identities.
The determinants of compliance VAT gap
Authors: Cuceu Ionuţ Constantin , Florescu Decebal Remus , Văidean Viorela Ligia 
Published: February 2024

PurposeThis paper aims to analyze the potential variables explaining the compliance value added tax (VAT) gap, which basically represents an estimate of the unpaid VAT in the economy. A major component of compliance VAT Gap is represented by tax fraud; there exist other causes too, like insolvencies, bankruptcies, optimizations practices and maladministration. The objective of our paper is to revisit the main determinants of the VAT compliance gap for the European Union (EU)-27 member states. Using econometric modeling, our study identifies the relationship between the VAT gap and various determinants of it.Design/methodology/approachOur work focuses on the shadow economy, final consumption, VAT revenues, standard VAT rates, differences between the standard and reduced rates, economic prosperity, press freedom, political stability and others, as determinants of European VAT compliance gaps, for the 2005–2020 time interval. The methods include panel data analysis through simple and multiple regression modeling, the combinatorial approach, fixed and random effects.FindingsOur study validates the direct impact of shadow economy and the indirect impact of VAT revenues, economic prosperity and press freedom, upon VAT compliance gaps. Upon subsampling of EU member states within old and new ones, our results estimate a larger positive impact of shadow economy upon old member states, compared to new ones.Practical implicationsThe policy implications include leverage effects of governments acting upon a reduction in shadow economy phenomena and boosts of economic development, political stability and press freedom, in order to attain the contraction of compliance VAT gaps.Originality/valueOur paper sheds light in a poorly explored scientific area, that of the determinants of VAT gap, especially in relationship with financial and economic crime phenomena.
An application of the extended UTAUT model to understand the adoption of cashless transactions: evidence from developing country
Authors: Sakib Md. Nazmus , Akter Mahmuda , Sahabuddin Mohammad , Fahlevi Mochammad 
Published: February 2024

Purpose
This study aims to identify the factors influencing cashless transactions toward digital payment systems using the extended UTAUT model in developing countries. This model was extended with perceived usefulness, perceived ease of use, facilitating conditions, perceived security/trust and social influence for assessing consumer behavior toward cashless transactions.

Design/methodology/approach
Using structural equation modeling (SEM), this study conducted a cross-sectional survey to collect data, providing a snapshot of the relationship between exogenous and endogenous variables.

Findings
The results of the study indicate that perceived usefulness, facilitating conditions, perceived trust/security and social influence have a significant influence on consumer intentions toward cashless transactions. Oppositely, leaving the perceived ease of use has no significant influence on consumer intentions toward the usage of cashless transactions.

Originality/value
The contribution of this study is to extend the UTAUT model for adopting cashless transactions in developing countries that will help government agencies, service providers and financial institutions design effective strategies in the future.
Resurgence and transformation: the impact of COVID-19 on Myanmar’s tourism industry and future trends
Authors: Zreik Mohamad 
Published: February 2024

PurposeThis paper analyses the impact of the COVID-19 pandemic on Myanmar’s tourism industry, identifying challenges and innovations, and predicting future trends to foster a resilient and sustainable sector.Design/methodology/approachThe study uses a comprehensive analysis of tourism trends, government and private sector responses and changes in tourist behaviour, employing data, case studies and policy reviews.FindingsThe pandemic significantly disrupted Myanmar’s tourism, causing economic losses and shifts towards sustainable, outdoor and cultural experiences. Effective strategies by the government and private sector are aiding recovery and suggesting a more diverse and resilient future.Research limitations/implicationsThe research is limited by the availability of data during the pandemic, highlighting the need for ongoing strategy adaptation.Practical implicationsThe study recommends digitalization, health protocols, domestic tourism focus and sustainable practices for a robust post-pandemic industry.Social implicationsThe study emphasizes the pandemic’s social impact on tourism-dependent communities and the need for inclusive, sustainable tourism practices.Originality/valueItprovides unique insights into COVID-19’s multifaceted impact on Myanmar’s tourism, offering valuable information for future sector strategies.
Pervasiveness of inorganic gunshot residue (IGSR) in handguns after cleaning and conditioning procedures
Authors: Donghi Matteo , Orsenigo Stefano , Manna Luigi , Profumo Antonella , Mattino Alessia , Merli Daniele 
Published: February 2024

AbstractMemory effect in firearms that is, the possibility for a weapon to release inorganic particles whose elemental composition depends on its entire shooting history, is responsible for most of the interpretation difficulties encountered in forensic gunshot residue analysis. The presence of residues chemically inconsistent with the last discharged round, the creation of particles having unusual elemental profiles, and the dependence of residue population composition on the collection point are all manifestations of memory effect. The experimental results reported in this paper highlight the ineffectiveness of a wide number of gun cleaning procedures in reducing memory effect. Moreover, the common alternative of discharging batches of rounds having a “new” primer mixture does not fully eliminate the possibility to recover “old” residues at least from the shooter's hands. Two brand new pistols and ammunition having lead‐based, leadless and heavy metal free primers were used. Specimens, collected both from the shooters' hands and from cotton targets set nearby the gun muzzle, were analyzed by SEM‐EDS and by ICP‐OES. After discharging 10's of new ammunitions, the number of old residues ejected from the gun muzzle indeed showed an asymptotic decrease to zero. In spite of this, the number of old residues recovered from the shooter's hands did not follow any predictable trend. These different behaviors suggest that all internal components of a gun, and not just the barrel, play a role in memory effect.