bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of SLC40A1, LCN2, CREB5, and SLC7A11 as ferroptosis-related biomarkers in alopecia areata through machine learning
Authors: Xu Wen , Wei Dongfan , Song Xiuzu 
Published: February 2024

AbstractAlopecia areata (AA) is a common non-scarring hair loss condition driven by the collapse of immune privilege and oxidative stress. The role of ferroptosis, a type of cell death linked to oxidative stress, in AA is yet to be explored, even though it's implicated in various diseases. Using transcriptome data from AA patients and controls from datasets GSE68801 and GSE80342, we aimed to identify AA diagnostic marker genes linked to ferroptosis. We employed Single-sample gene set enrichment analysis (ssGSEA) for immune cell infiltration evaluation. Correlations between ferroptosis-related differentially expressed genes (FRDEGs) and immune cells/functions were identified using Spearman analysis. Feature selection was done through Support vector machine-recursive feature elimination (SVM-RFE) and LASSO regression models. Validation was performed using the GSE80342 dataset, followed by hierarchical internal validation. We also constructed a nomogram to assess the predictive ability of FRDEGs in AA. Furthermore, the expression and distribution of these molecules were confirmed through immunofluorescence. Four genes, namely SLC40A1, LCN2, CREB5, and SLC7A11, were identified as markers for AA. A prediction model based on these genes showed high accuracy (AUC = 0.9052). Immunofluorescence revealed reduced expression of these molecules in AA patients compared to normal controls (NC), with SLC40A1 and CREB5 showing significant differences. Notably, they were primarily localized to the outer root sheath and in proximity to the sebaceous glands. Our study identified several ferroptosis-related genes associated with AA. These findings, emerging from the integration of immune cell infiltration analysis and machine learning, contribute to the evolving understanding of diagnostic and therapeutic strategies in AA. Importantly, this research lays a solid foundation for subsequent studies exploring the intricate relationship between AA and ferroptosis.
Cancer-related cells and oncosomes in the liquid biopsy of pancreatic cancer patients undergoing surgery
Authors: Shishido Stephanie N. , Lin Emmeline , Nissen Nicholas , Courcoubetis George , Suresh Divya , Mason Jeremy , Osipov Arsen , Hendifar Andrew E. , Lewis Michael , Gaddam Srinivas , Pandol Stephen , Kuhn Peter , Lo Simon K. 
Published: February 2024

AbstractPancreatic ductal adenocarcinoma (PDAC) has a five-year survival rate of less than 10% due to its late diagnosis, rapid metastasis, and chemotherapeutic resistance. For a small proportion (10–20%) of early-stage patients however, surgical resection of the pancreatic tumor offers the best chance for survival but the effect of surgery on disease dissemination is unknown. The primary objective of this study was to characterize cellular and acellular blood-based analytes in portal and peripheral blood before pancreatic manipulation, during tumor dissection and immediately after surgical resection to determine the effects of the surgery. This study used the non-enriching third generation High-Definition Single Cell Assay (HDSCA3.0) workflow to investigate heterogeneous circulating rare cell population in the blood. Blood from both sites taken before surgical manipulation of the pancreas had significantly greater incidence of total rare cellular and acellular analytes than normal donor samples. Post-surgery portal and peripheral blood had significantly greater incidence of specific cellular and acellular subtypes compared to the matched pre- and during-surgery samples. Our results reveal that in patients with PDAC liquid biopsy analytes are increased in both the portal and peripheral blood; portal blood contains a higher frequency of analytes than in the peripheral blood; total analytes in the portal and peripheral blood samples were significantly associated with the tumor volume and pathological T stage; and the surgical procedure increased the blood levels of circulating cellular and acellular analytes, but not Epi.CTCs or Mes.CTCs. This study demonstrates liquid biopsy’s utility in monitoring patients with PDAC with surgically resectable disease.
Bob1 maintains T follicular helper cells for long-term humoral immunity
Authors: Yanagi Masahiro , Ikegami Ippei , Kamekura Ryuta , Sato Tatsuya , Sato Taiki , Kamiya Shiori , Murayama Kosuke , Jitsukawa Sumito , Ito Fumie , Yorozu Akira , Kihara Miho , Abe Takaya , Takaki Hiromi , Kawata Koji , Shigehara Katsunori , Miyajima Satsuki , Nishikiori Hirotaka , Sato Akinori , Tohse Noritsugu , Takano Ken-ichi , Chiba Hirofumi , Ichimiya Shingo 
Published: February 2024

AbstractHumoral immunity is vital for host protection, yet aberrant antibody responses can trigger harmful inflammation and immune-related disorders. T follicular helper (Tfh) cells, central to humoral immunity, have garnered significant attention for unraveling immune mechanisms. This study shows the role of B-cell Oct-binding protein 1 (Bob1), a transcriptional coactivator, in Tfh cell regulation. Our investigation, utilizing conditional Bob1-deficient mice, suggests that Bob1 plays a critical role in modulating inducible T-cell costimulator expression and cellular respiration in Tfh cells. This regulation maintains the long-term functionality of Tfh cells, enabling their reactivation from central memory T cells to produce antibodies during recall responses. In a bronchial asthma model induced by house dust mite (HDM) inhalation, Bob1 is observed to enhance HDM-specific antibodies, including IgE, highlighting its pivotal function in Tfh cell regulation. Further exploration of Bob1-dependent mechanisms in Tfh cells holds promise for governing protective immunity and addressing immune-related disorders.
Maximum tree height in European Mountains decreases above a climate-related elevation threshold
Authors: Gelabert P. J. , Rodrigues M. , Coll L. , Vega-Garcia C. , Ameztegui A. 
Published: February 2024

AbstractMountain forests face important threats from global change and spatio-temporal variation in tree height can help to monitor these effects. In this study, we used the Global Ecosystem Dynamics Investigation space-borne laser sensor to examine the relationship between maximum tree height and elevation, and the role of climate, in the main European mountain ranges. We found a piecewise relationship between elevation and maximum tree height in all mountain ranges, supporting the existence of a common breakpoint that marks the beginning of tree development limitations. Temperature and precipitation were identified as the most important drivers of tree height variation. Additionally, we predicted significant upward displacement of the breakpoint for the period 2080-2100 under climate change scenarios, potentially increasing the area without growth limitations for trees. These findings contribute to understanding the impacts of global warming on mountain forest ecosystems and provide insights for their monitoring and management.
METTL3 promotes osteogenic differentiation of human umbilical cord mesenchymal stem cells by up-regulating m6A modification of circCTTN
Authors: Chen Shujiang , Duan Xiaoqiong , He Yanjin , Chen Wenchuan 
Published: February 2024

Abstract
Background: Human umbilical cord mesenchymal stem cells (hUCMSCs) are promising seed cells in bone tissue engineering. circRNA and N6-methyladenosine (m6A) RNA methylation play important roles in osteogenic differentiation. Here, we investigated the potential relevance of a critical circRNA, hsa_circ_0003376 (circCTTN), and methyltransferase-like 3 (METTL3) in osteogenic differentiation of hUCMSCs.
Methods: Expression of circCTTN after hUCMSC osteogenic induction was detected by qRT-PCR. Three databases (RMBase v2.0, BERMP, and SRAMP) were used to predict m6A sites of circCTTN. RNA was enriched by methylated RNA immunoprecipitation (MeRIP), followed by quantitative real-time polymerase chain reaction to detect m6A level of circCTTN after METTL3 overexpression and osteogenic induction. RNA pull-down, Western blotting, and protein mass spectrometry were performed to investigate the potential mechanisms by which METTL3 promoted m6A modification of circCTTN. Bioinformatic analyses based on database (STRING) search and co-immunoprecipitation were used to analyze the proteins that interacted with METTL3.
Results: Overexpression of METTL3 promoted osteogenic differentiation of hUCMSCs and increased m6A level of circCTTN. Two potential m6A modification sites of circCTTN were predicted. No direct interaction between METTL3 and circCTTN was observed. Thirty-one proteins were pulled down by probes specific for circCTTN, including NOP2, and two m6A reading proteins, EIF3A and SND1. Bioinformatics analysis and co-immunoprecipitation showed that METTL3 interacted with EIF3A indirectly through NOP2.
Conclusions: METTL3 promotes the osteogenic differentiation of hUCMSCs by increasing the m6A level of circCTTN. However, METTL3 does not bind directly to circCTTN. METTL3 interacts with circCTTN indirectly through NOP2 and EIF3A.
Evaluation of an Integrated Fluency and Acceptance and Commitment Therapy Intervention for Adolescents and Adults Who Stutter
Authors: Hart Alice K. , Breen Lauren J. , Hennessey Neville W. , Beilby Janet M. 
Published: February 2024

Purpose:
Developmental stuttering is a complex and multifaceted neurodevelopmental disorder that may cause pervasive negative consequences for adults who stutter (AWS). Historically, intervention for AWS has primarily addressed speech fluency, with less focus on the covert psychosocial aspects of the disorder. The purpose of this article is to report on a feasibility trial evaluating a novel integrated intervention that combines traditional stuttering management techniques with Acceptance and Commitment Therapy (ACT) for AWS.

Method:
Twenty-nine AWS participated in the feasibility trial. All participants successfully completed a combined fluency and ACT intervention, titled the fluency and Acceptance and Commitment Therapy for Stuttering (fACTS) Program. As this was a feasibility study, no control group was included. Intervention was administered by two certified practicing speech-language pathologists, over eight 60- to 90-min sessions.

Results:
Generalized linear mixed modeling was used to determine change from pre- to post-intervention and follow-up. Significant pre- and post-intervention improvements in self-efficacy, psychosocial functioning, and psychological flexibility were observed, along with significant reductions in observable stuttering behaviors (i.e., stuttered speech frequency). Intervention gains for all variables of interest were maintained 3 and 6 months post-intervention.

Conclusions:
The fACTS Program was created to be a holistic and flexible intervention to promote self-efficacy beliefs and address stuttering-related psychosocial impacts and speech fluency goals of AWS. Preliminary results indicated positive improvement in all psychosocial outcomes (i.e., self-efficacy, psychosocial impact, and psychological flexibility) and observable speech fluency following completion of the program. Future clinical trials of the fACTS Program with an included control group will further investigate the mechanisms of change for the positive effects observed.
MiRNA Signatures Related to Invasiveness and Recurrence in Patients
With Non-Functioning Pituitary Neuroendocrine Tumors
Authors: Nikolova Emiliya , Nankova Anelia , Kalenderova Silvia , Georgieva Bilyana , Hadzhiyanev Asen , Bichev Stoyan , Savov Alexey , Todorova Albena , Mitev Vanyo , Elenkova Atanaska 
Published: February 2024

Abstract
Purpose: This preliminary study aimed to analyze and identify
differentially expressed miRNAs in Bulgarian patients with non-functioning
pituitary neuroendocrine tumors (NFPitNET). The relationship between deregulated
miRNAs and tumor invasiveness, recurrence, and size was determined.
Methods: Twenty patients with NFPitNET were selected and fresh pituitary
tumor tissues were collected. RNA containing miRNAs were isolated using
miRNAeasy mini kit and analyzed by quantitative real-time polymerase chain
reaction (PCR) using LNA miRNA Cancer-Focus PCR Panel
(Qiagen).
Results: Three miRNAs (miR-210-3p, miR-149-3p, and miR-29b-3p) were
deregulated in invasive compared to non-invasive NFPitNETs. Differential
expression of four-miRNA signatures – miRNA-17, miR-19, miR-106a, and miR-20,
correlated with patient recurrence.
Conclusion: This prospective pilot study selected a unique miRNA
expression profile, that correlates with invasiveness and recurrence in
non-functioning pituitary neuroendocrine tumors. Moreover, some of the selected
miRNAs are reported for the first time in patients with this disease, shedding
light on the molecular mechanisms involved in pituitary pathogenesis. The
identified miRNAs demonstrate potential as biomarkers, deserving further
investigation in a larger cohort to validate their clinical applicability.
Enzymatic Synthesis of Hydroxycinnamic Acid Amides in Water Using the Promiscuous Hydrolase/Acyltransferase PestE from Pyrobaculum calidifontis VA1
Authors: Bornscheuer Uwe T. , Baumert Benjamin , Terholsen Henrik , Cziegler Clemens , Thier Isabel , Badenhorst Christoffel P. S. , Siems Karsten 
Published: February 2024

AbstractHydroxycinnamic acid amides are believed to have antioxidant, antidiabetic, cytotoxic, anticancer, neuroprotective, and antiinflammatory properties, making them interesting target molecules for potential applications in the food, cosmetics, and pharmaceutical industries. Here, we describe the synthesis of hydroxycinnamic acid amides starting from hydroxycinnamic acid esters and the corresponding amines by using variants of the promiscuous hydrolase/acyltransferase from Pyrobaculum calidifontis VA1 (PestE) in water as a solvent. Up to 97% conversion within two hours at 60 °C was achieved with methyl ferulate and tyramine as substrates. This is a promising, environmentally friendly alternative strategy to established chemical synthesis routes or enzymatic methods using lipases in nonaqueous organic solvents.
Synthesis of Acyl Hydrazides and Hydrazones from Activated Amides
Authors: Lee Sunwoo , Kim Miso 
Published: February 2024

AbstractAcyl hydrazide and acyl hydrazone synthesis methods were developed. They involve the reaction of an activated amide with hydrazine under transition-metal-catalyst-free conditions. Additionally, acyl hydrazones were synthesized by extending the same reaction procedure in the presence of aldehydes (or ketones). These reactions were performed in an aqueous environment at 25 °C, demonstrating the successful attainment of the desired compounds with high yields.
Synthesis of Phosphinoboranes via Coordination-Induced Bromine Abstraction of an Isolable Phosphinyl Radical with Bromoboranes
Authors: Ishida Shintaro , Iwamoto Takeaki , Katayama Yasuhiro , Hirakawa Fumiya 
Published: February 2024

AbstractA stable dialkylphosphinyl radical reacted with bromoboranes to afford the corresponding phosphinoboranes accompanied with a bromophosphine under neutral condition at room temperature. The obtained phosphinoboranes have almost planar structures and have an effective P–B dative π-bond. The formation of phosphinoboranes would proceed via coordination-induced bromine abstraction from bromoboranes as a pivotal step, which was supported by the computational study.
Modeling the intention to consume and willingness to pay premium price for 3D-printed food in an emerging economy
Authors: Yang Marvello , Gao Jingzu , Yang Qing , Al Mamun Abdullah , Masukujjaman Mohammad , Hoque Mohammad Enamul 
Published: February 2024

AbstractThis study examined the factors influencing customers’ consumption intentions and willingness to pay a premium price for 3D-printed food by combining personal innovativeness, perceived compatibility, perceived product value, desire to eat, and willingness to pay a premium price for 3D-printed food into a conceptual framework. Applying the extended theory of planned behavior (TPB) framework with personal innovativeness, perceived compatibility, and perceived product value, this study obtained data from 1540 respondents using an online cross-sectional questionnaire and utilized partial least squares structural equation modeling to verify the study hypotheses. The findings revealed that personal inventiveness, attitude, perceived compatibility, subjective norms, perceived product value, and perceived behavioral control exert a significant influence on the intention and willingness to pay a premium price for 3D-printed food. The result also showed that perceived behavior and intention significantly affect willingness to pay a premium price for 3D-printed food. Furthermore, the outcome confirmed that, with the exception of the link between perceived product value, subjective norms, and willingness to pay a premium price for 3D-printed food, intention serves as a significant mediator in the relationship between factors and willingness to pay a premium price for 3D-printed food. This research contributes to the theoretical understanding of the factors that influence consumers’ intention to consume and willingness to pay a premium for 3D-printed food, providing new insights from new variables and broadening the scope of the TPB’s original model in the perspective of new food settings and Asian nations. These findings imply that 3D-printed food sellers in the Asian market need to pay more attention to the factors that stimulate customers’ intentions, which, in turn, affect consumers’ views of perceived product value and improve their readiness to pay a premium price for 3D-printed food.
Permanent or temporary homes?
Authors: Gonçalves Kellie , Lexander Kristin Vold 
Published: February 2024

AbstractQuestions surrounding mobility and migration are often connected to matters of language, citizenship, socio-economic status and class that are inherently unequal between developed and less-developed nation states. Scholars of geography and demographics have conceptualized both internal and external/international migration with “push” and “pull” factors as salient reasons for mobility. Traditional causes of external migration and so-called “push factors” include conflict, natural disaster, economic collapse, war, and transformations of socio-political systems as well as more promising employment options and decent incomes as contributing factors to external migration (Gonçalves & Schluter, 2020, p. 3). Such “forced moves” affect largely disadvantaged populations that are at risk (i.e. asylum seekers, refugees, victims of human trafficking) (Castles, 2003). For individuals engaging in lifestyle migration and lifestyle mobilities and considered voluntary migration, their life circumstances are extremely different in that they are often equipped with “privileged preconditions” (Mancinelli, 2020, p. 419) as “nomads from affluence” (Cohen, 1973) including for the most part, education, specialized skills, high standards of living and favorable visa regimes of their home countries (primarily in the West). These factors facilitate a range of choices in terms of where they travel to (i.e. place), also known as network capital, the kind of work they engage in and the rate of their mobility. Based on years of ethnographic work, in this paper, we investigate the different dimensions and discourses of lifestyle migration and lifestyle mobilities, both of which we argue presents a new trajectory and venue to explore within the field of migration linguistics (Borlongan, 2023). By focusing on two different case studies within Norway, we also draw on the notion of mediational repertoires (Lexander & Androutsopolous, 2021) as part of the communicative ecology in which jobs are found and where communication takes place among different types of migrants and their new permanent or temporary homes.
Flirting and winking in Tinder chats
Authors: Gibson Will 
Published: February 2024

Abstract
Existing research across the diverse field(s) of ‘discourse studies’ has started to explore the communicative
orders and sequential practices surrounding emoji use (Skovholt, Grønning and Kankaanranta
2014; Herring and Dainas 2017; Gibson,
Huang and Yu 2018; Sampietro 2019). However, researchers have not yet
systematically analysed one of the demonstrable phenomena of emoji, which is their ambiguity as meaning-making devices (Miller et al. 2016; Jaeger et al. 2017). This
study draws on Conversation Analysis to explore the issue of ambiguity in the use of one particular type of emoji, the wink (e.g.,
😉, 🥴, 😜). Drawing on a data corpus of text conversations in Danish and in Spanish by users of the dating app ‘Tinder’, the
analysis explores the phenomena of ambiguity in relation to the practice of flirting. The paper highlights four possible sources
of ambiguity: idiosyncratic use, semiotic references, sequential placement, and relationship to ambiguous textual actions. The
paper ends with reflections on possible future areas of research in the study of emoji and communication.
Extrinsic sources of translator job satisfaction
Authors: Rodríguez-Castro Mónica 
Published: February 2024

Abstract
Over the last decade, translator job satisfaction has received an increasing amount of attention in Translation
Studies. Translator job satisfaction is broadly investigated from different perspectives such as psychology, sociology, and
ergonomics. This article examines extrinsic sources of satisfaction that translators experience in evolving work environments and
revisits a comprehensive construct of job satisfaction (Rodríguez-Castro 2015) that
captures perceptions aligned with (a) the job (individual-job fit) and (b) the organization (individual-organization fit). This
construct includes multiple factors of job satisfaction that have not been extensively studied in the literature such as client
review and feedback, team interactions, communication workflow, and factors associated with online platforms. Such a construct
could be used to determine specific predictors of job satisfaction for devising career pathways to enhance retention and improve
overall human resource management in the language industry.
A novel robust intelligent fault diagnosis method for rolling bearings based on SPAVMD and WOA-LSSVM under noisy conditions
Authors: Yan Xiaoan , Hua Xing , Jiang Dong , Xiang Ling 
Published: February 2024

Abstract
Aiming at the problem that the fault information of rolling bearings under harsh operation environment is easily submerged by strong noise interference, which causes the traditional method to be difficult to identify bearing faults effectively, this paper proposes a novel robust intelligent fault diagnosis method for rolling bearings based on sparsity-assisted parameter adjustable variational mode decomposition (VMD) and whale optimization algorithm-based optimized least-squares support vector machine (WOA-LSSVM). Firstly, a sparsity measurement named the improved Gini index is introduced as the fitness function of grid search algorithm to adaptively adjust and search for the optimal decomposed mode number K and penalty factor α of VMD. Additionally, VMD containing the optimal parameters is adopted to decompose the original bearing vibration signal into several intrinsic mode function (IMF), and the effective signal reconstruction is performed by screening the sensitive IMF components according to the effective weighted kurtosis Gini index criterion. Subsequently, the refine composite multi-scale dispersion entropy of the reconstructed signal is further calculated to establish a multi-dimensional feature vector set. Finally, the constructed feature vector set is fed into the WOA-LSSVM to achieve automatic fault identification of rolling bearings. The effectiveness of the proposed method is verified by two experimental examples. Experimental results show that the proposed method has higher fault recognition accuracy and better robustness against noise than other homologous methods in noisy conditions. This study provides a new perspective for the developing of robust diagnosis methods.