bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between Optical Coherence Tomography Ganglion Cell Layer Analysis and Visual Field in Cases of Benign Intracranial Hypertension
Authors: Saad Elham Abdullah Elsheraiy , Mohammed Tarek Ragaey , Soht Rabab Mohammed El , Abdelhalim Tamer Ibrahim 
Published: February 2024

Background: Benign intracranial hypertension (BIH) is a condition in which there is excessive cerebral spinal fluid (CSF) pressure in the subarachnoid space that surrounds the brain and spinal cord (intracranial pressure (ICP)) owing to an unexplained cause. It affects 1:100,000 persons yearly with a 20 times greater prevalence in young, obese girls. We wanted to correlate between optical coherence tomography ganglion cell layer analyses and visual field in patients of benign intracranial hypertension.
Methods: This cross-sectional clinical study that was carried out on 30 patients (60 eyes) who all had a recently diagnosed papilledema due to first episode of IIH. Goal of the study, and applied tests was done before obtaining an informed consent. Personal history, past medical and ocular history, possible risk factors for IIH were taken. Full ophthalmological examination including best corrected visual acuity (BCVA), intraocular pressure (IOP), color perception, pupillary reflexes, slit lamp bio microscopy examination including assessment of papilledema grade, blood pressure check and body mass index (BMI). All patients underwent automated perimetry in dim light to evaluate their visual field using SITA fast Standard 30-2 protocol by Humphrey Field Analyzer (Humphrey Field Analyzer I Carl Zeiss AG – Oberkochen – Germany).
Results: Average RNFL thickness had significant positive correlation with rim area (r=0.654, P<0.001) and disc area (r=0.665, P<0.001). Average RNFL thickness had no correlation with VA, IOP and average CD.
Average GCL complex had significant negative correlation with rim area (r= -0.283, P=0.028) and disc area (r= -0.328, P=0.01). Average GCL complex had no correlation with VA, IOP and average CD. Minimum GCL complex had significant negative correlation with rim area (r= -0.272, P=0.036) and disc area (r= -0.315, P=0.014). Minimum GCL complex had no correlation with VA, IOP and average CD. Papilledema grading had significant positive correlation with average RNFL thickness (r=0.461, P<0.001). Papilledema grading had no correlation with average GCL complex, minimum GCL complex, VFI, MD and PSD.
Conclusions: There is a strong structure-function link between visual field indices and OCT parameters such as RNFL and GCC.
Organization of mitochondrial UDP-glucose: Dolichylmonophosphate glucosyltransferase in the outer membrane
Authors: Gasnier F. , Lerme F. , Vaganay E. , Louisot P. , Gateau-Roesch O. 
Published: February 2005

Previous studies have shown the existence of an autonomous mitochondrial UDP-glucose: dolichylmonophosphate glucosyltransferase, located in mitochondrial outer membrane of liver cells. To improve our knowledge about the topographical aspects of glycosylation in mitochondria, we have investigated the organization of this enzyme in intact mitochondria, using controlled proteolysis with trypsin and sensitivity towards amino-acid specific reagents.
Our data provides evidence:-for a mitochondrial glucosyltransferase facing the cytoplasmic side of the outer membrane-and for the involvement of histidine and tryptophan residues as well as sulfhydryl groups in the catalytic activity of the enzyme.
Electrorotation of lymphocytes—The influence of membrane events and nucleus
Authors: Ziervogel Heiko , Glaser Roland , Schadow Dieter , Heymann Stephan 
Published: February 2005

Electrorotation—the spin of cells in rotating high frequency electric fields—has been used to investigate properties of human peripheral blood lymphocytes. The rotation spectra of lymphocytes deviate from those of single shell spheres. The deviations are caused by the electrical properties of the nucleus in the cell interior.
Electrorotation allows the distinction between successfully stimulated lymphocytes and unstimulated cells after application of concanavalin A. Notwithstanding the fact that only a proportion of the cells will be mitogenically stimulated we detected an enhanced cell membrane conductivity for the whole cell population immediately after the addition of mitogen.
Detection of protein disulphide-isomerase in sheep pancreas fractions
Authors: Hillson David A. , Anderson Jacqueline 
Published: February 2005

Conclusions
The use of diethylpyrocarbonate to inhibit endogenous ribonuclease in sheep pancreas allows the detection of protein-disulphide-isomerase activity in homogenates, at specific activities of up to 4 units/g. This is higher than the specific activity in sheep liver homogenates (about 2 units/g) or in homogenates of other sheep tissues (16). It is thus evident that high levels of protein-disulphide-isomerase activity are present in sheep pancreas. This is consistent both with the postulated general role of protein disulphide-isomerase in protein biosynthesis (10,11) and with the in vitro action of the enzyme on its conventional substrate scrambled ribonuclease, since pancreas is the major site of ribonuclease synthesis.
Calcium binding protein changes of sarcoplasmic reticulum from rat denervated skeletal muscle
Authors: Loirat Marie-Jeanne , Lucas-Heron Brigitte , Ollivier Béatrice , Leoty Claude 
Published: February 2005

Two Ca2+ sequestering proteins were studied in fast-twitch (EDL) and slow-twitch (soleus) muscle sarcoplasmic reticulum (SR) as a function of denervation time. Ca2+-ATPase activity measured in SR fractions of normal soleus represented 5% of that measure in SR fractions of normal EDL. Denervation caused a severe decrease in activity only in fast-twich muscle. Ca2+-ATPase and calsequestrin contents were affected differently by denervation. In EDL SR, Ca2+-ATPase content decreased progressively, whereas in soleus SR, no variation was observed. Calsequestrin showed a slight increase in both muscles as a function of denervation time correlated with increased45Ca-binding.
These results indicate first that Ca2+-ATPase activity in EDL was under neural control, and that because of low Ca2+-ATPase activity and content in slow-twitch muscle no variation could be detected, and secondly that greater calsequestrin content might represent a relative increasing of heavy vesicles or decreasing of light vesicles as a function of denervation time in the whole SR fraction isolated in both types of muscles.
Introduction of antibody (PL/IM 430) to a 100 kDa protein into permeabilised platelets inhibits intracellular sequestration of Ca2+
Authors: Hack Nashrudeen , Authi Kalwant Singh , Crawford Neville 
Published: February 2005

A monoclonal antibody (PL/IM 430), previously found to inhibit the uptake of Ca2+ into highly purified platelet intracellular membrane vesicles (Hack, N., Wilkinson, J. M. and Crawford, N. 1988, Biochem. J.250, 355–361) has been introduced into saponin-permeabilised platelets. At a saponin concentration (20–25 μg/ml) commensurate with total LDH release, sequestration of Ca2+ into intracellular non-mitochondrial stores is inhibited by the antibody (~50% inhibition at 20 μg/ml IgG). At higher saponin concentrations when intracellular binding of125I-labelled mAb is maximum, inhibition of Ca2+ sequestration approaches 70%. The inhibition is specific, control studies with non-platelet directed mouse IgG and mAbs which immunoblot platelet antigens other than the 100 kDa protein did not affect the Ca2+ sequestration.
No effect of the antibody were observed against IP3-induced release of prestored Ca2+, either in permeabilised platelets or with isolated intracellular membrane vesicles. The mAb PL/IM 430 appears to bind only to the Ca2+ translocating channel protein associated with the intracellular membrane (Ca2++Mg2+) ATPase and not to Ca2+ channels responsive to IP3.
Intrinsic fluorescence studies on saccharide binding toArtocarpus integrifolialectin
Authors: Sastry M. V. Krishna , Surolia A. 
Published: February 2005

The combining region of Artocarpus integrifolia lectin has been studied by using the ligand-induced changes in the fluorescence of the lectin. The saccharide binding properties of the lectin show that C-l, C-2, C-4, and C-6 hydroxyl groups of D-galactose are important loci for sugar binding. The α-anorner of galactose binds more strongly than its β-counterpart. Inversion in the configuration at C-4 as in glucose results in a loss of binding to the lectin. The C-6 hydroxyl group is also presumably involved in binding as D-fucose does not bind to the lectin.The lectin binds to the Thomsen-Friedenreich antigen (Galβ(1→3)GalNAc) more strongly than the other disaccharides studied, viz. Gal/β (1→4) Gal and Galβ (1→3) GlcNAc, which are topographically similar to T-antigen. This observation suggests that the combining region of Artocarpus lectin is complementary to that of T-antigen.Solvent accessibility of the protein fluorophores have been probed by the quenching of protein fluorescence by Iodide ion in the absence and presence of sugar. In the presence of sugar a slight inaccessibility of the fluorophores to the solvent has been observed.
Regulation of acyl CoA: Cholesterol acyl transferase (ACAT) activity by mevalonate and cholesterol in isolated rat hepatocytes during perinatal development
Authors: Bruscalupi G. , Devirgiliis L. Conti , Leoni S. , Piemonte F. , Trentalance A. 
Published: February 2005

Acyl CoA: cholesterol acyl transferase (ACAT) activity presents marked oscillations and differential sensitivity to the “in vitro” stimulation of the kinase-phosphatase modulatory system in the perinatal rat liver.
The regulation of this enzyme activity by some modulators generally active in adulthood, such as cholesterol, lipoproteins and mevalonate, has been studied in hepatocytes isolated at different developmental stages. A lack of effect of mevalonate and a positive effort of lipoprotein cholesterol have been observed at the fetal and neonatal stages.
A differential prevalence is suggested of one of the two modulatory mechanisms (phosphorylation-dephosphorylation system, or substrate effect) at each developmental stage.
Myelin Basic Protein inhibits the calcium response to phytohaemagglutinin in human lymphocytes
Authors: Bellini Tiziana , Degani Diana , Matteuzzi Maurizio , Dallocchio Franco 
Published: February 2005

Myelin Basic Protein, one of the major membrane protein component of the central nervous system, was used to probe the molecular mechanism of cellular activation by phytohaemagglutinin.
Pre-treatment of human lymphocytes with myelin basic protein results in a lower rising of cytosolic concentration of free calcium after stimulation with phytohaemagglutinin.
This effect is dependent on myelin basic protein concentration and on the preincubation time of the protein with the cells. It is not due to a interaction between myelin basic protein and phytohaemagglutinin, but appears to be a consequence of the binding of the protein to the cell surface.
The reduction of the rise of cytosolic calcium induced by phytohaemagglutinin is specific for the myelin basic protein because other proteins like albumin and protamine have no effect.
Effects of mitogenic agents upon glucocorticoid action in human tonsillar T-lymphocytes
Authors: Altiok S. , Bermek E. 
Published: February 2005

The treatment of human tonsillar T-lymphocytes with 4-phorbol 12-myristate 13-acetate (PMA), resulted in about two fold increase in glucocorticoid receptor (GR) number, without any significant change in the receptor affinity. This increase disappeared in the presence of cycloheximide.Alone, PMA and calcium inophore A23187 did not affect, but together stimulated, like phytohaemagglutinin (PHA), leucine and, in particular, thymidine incorporation. PMA enhanced slightly the stimulatory effect of PHA. Alone, these agents failed to alter the suppressive effect of dexamethasone on thymidine and leucine incorporation; however, PMA-A23187 and PMA-PHA combinations appeared to antagonize the supression by dexamethasone.
The major protein of bull seminal plasma: Biosynthesis and biological function
Authors: Scheit Karl Heinz , Kemme Michael , Aumüller Gerhard , Seitz Jürgen , Hagendorff Gerd , Zimmer Michael 
Published: February 2005

We isolated the major protein of apparent Mr of 15,000–16,000 from seminal plasma as well as from seminal veiscle secretion of bull and proved by amino acid analysis and tryptic peptide mapping that the two proteins were identical. An antiserum against this major protein was employed to quantitate and identify the major protein in seminal plasma as well as seminal vesicle secretion. The antiserum did not cross-react with proteins from bovine or human plasma or follicular fluid respectively.
Cell-free translation of poly(A)RNA from seminal vesicle tissue and immunoprecipitation yielded one major species with apparent Mr of 18,000. Using the anti-major protein antiserum, this major species was specifically immuno absorbed. Cloning and sequencing of a major protein-specific cDNA led to the identification of clone pMP17, encoding a precursor of the major protein of 128 amino acid residues. We proved that the major protein is identical to protein PDC 109 (Esch et al., Biochem. Biophys. Res. Comm.113:861–867, 1983).
The seminal vesicles synthesize major protein in an androgen-dependent fashion. In addition to intraluminal secretion of the vas deferens, ampullary spermatozoa revealed an intense immunoreaction which was restricted to the neck region of the sperm head and the middle piece, while the principal piece of the tail as well as the sperm head were devoid of immunoreactive material. Epididymal epithelium (as well as calf seminal vesicle epithelium) showed no immunoreactivity with major protein antiserum. Immunoelectron microscopy demonstrated that only spermatozoa devoid of a plasma membrane around the middle piece were able to bind the antiserum against major protein. After removal of the plasma membrane from epididymal spermatozoa, binding of major protein to subplasmalemmal binding sites was visualised using gold-labeled MP.
Transblotting with gold-labeled MP demonstrated a protein of about 66 kDa which appears to represent the major protein-receptor. Binding of major protein to the receptor (after loss of the plasma membrane in the mid-piece region of the spermatozoa after contact with secretions from seminal vesicles) is interpreted as a phyisological process presumably related to the onset of sperm motility.
Influence of lindane on the fluidity of the rat ventral prostate membranes
Authors: Senar S. , Gutierrez-Ocaña M. T. , Perez-Albarsanz M. A. , Recio M. N. 
Published: February 2005

The influence of lindane upon the dynamic properties of plasma membranes from rat ventral prostate has been investigated using a fluorescence polarization technique. Preincubation with lindane decreased the fluorescence polarization in a dose dependent manner. This effect, which is associated with an increased membrane fluidity, occurred in a very short period of time.
Lindane also provoked a number of changes in lipid biosynthesis from acetate in the membrane. Less [1-14C]acetate was incorporated into cholesterol and more into phospholipids when this liposoluble toxicant was added to the preincubation medium. However, not all phospholipid classes were equally increased, because while the rate of acetate incorporation was greater into choline glycerophospholipids than into ethanolamine glycerophospholipids, both were higher than the rates of acetate incorporation into serine glycerophospholipids and sphingomyelin.
Regulation of polyamine biosynthesis by antizyme and some recent developments relating the induction of polyamine biosynthesis to cell growth
Authors: Canellakis E. S. , Kyriakidis D. A. , Rinehart C. A. , Huang S.-C. , Panagiotidis C. , Fong W.-F. 
Published: February 2005

This review considers the role of antizyme, of amino acids and of protein synthesis in the regulation of polyamine biosynthesis.
The ornithine decarboxylase of eukaryotic ceils and of Escherichia coli coli can be non-competitively inhibited by proteins, termed antizymes, which are induced by di-and poly- amines. Some antizymes have been purified to homogeneity and have been shown to be structurally unique to the cell of origin. Yet, the E. coli antizyme and the rat liver antizyme cross react and inhibit each other's biosynthetic decarboxylases. These results indicate that aspects of the control of polyamine biosynthesis have been highly conserved throughout evolution.
Evidence for the physiological role of the antizyme in mammalian cells rests upon its identification in normal uninduced cells, upon the inverse relationship that exists between antizyme and ornithine decarboxylase as well as upon the existence of the complex of ornithine decarboxylase and antizyme in vivo. Furthermore, the antizyme has been shown to be highly specific; its Keq for ornithine decarboxylase is 1.4 × 1011 M-1. In addition, mammalian ceils contain an anti-antizyme, a protein that specifically binds to the antizyme of an ornithine decarboxylase-antizyme complex and liberates free ornithine decarboxylase from the complex. In B. coli, in which polyamine biosynthesis is mediated both by ornithine decarboxylase and by arginine decarboxylase, three proteins (one acidic and two basic) have been purified, each of which inhibits both these enzymes. They do not inhibit the biodegradative ornithine and arginine decarboxylases nor lysine decarboxylase. The two basic inhibitors have been shown to correspond to the ribosomal proteins S20/L26 and L34, respectively. The relationship of the acidic antizyme to other known B. coli proteins remains to be determined.
Rat carcinoma cells in long-term, serum-free culture provide a continuing supply of collagenase
Authors: Stevenson Geoffrey A. , Lyons J. Guy , Cameron David A. , O'Grady Robert L. 
Published: February 2005

Neoplastic, epithelial cells derived from a spontaneously-arising rat mammary carcinoma have been cultured in a defined medium, in the absence of serum, continuously, for over 2 years. The medium is a mixture of Ham's F12 and Dulbecco's Modified Eagle's media supplemented with insulin, transferrin and bovine serum albumin. The cells have retained their potential to produce tumours and, in culture, a true vertebrate collagenase.
This system provides a continuing supply of vertebrate collagenase through the application of recently developed methods.
Washout phenomena in dialyzed mast cells allow discrimination of different steps in stimulus-secretion coupling
Authors: Penner Reinhold , Pusch Michael , Neher Erwin 
Published: February 2005

Transient increases of intracellular calcium and exocytotic activity of rat peritoneal mast cells following stimulation with compound 48/80 were monitored using the Ca-indicator dye fura-2 and the capacitance measurement technique. It is known that mast cells very rapidly lose their secretory response towards antigenic or compound 48/80-induced stimulation in the whole-cell recording configuration of the patch-clamp technique due to “washout” of signal mediators. In contrast, we found that calcium transients remained unaffected by intracellular dialysis for as long as 10 min.The fast “washout” phenomenon of exocytosis could be overcome by supplementing the pipette filling solution with guanosinetriphosphate (GTP) indicating a major role for GTP-binding proteins in secretion. The restoration of exocytosis was transient and decayed within three minutes, suggesting diffusional escape of one or several other cytoplasmic substances involved in stimulus-secretion coupling. Quantitative aspects of this process and the implications of its differential effects on Ca-transients versus secretion are discussed.
Metabolic studies of whole animals and humans using phosphorus nuclear magnetic resonance
Authors: Gadian David G. 
Published: February 2005

Conclusions
31P NMR now provides a totally non-invasive method of studying the metabolism of selected tissues and organs within whole animals and humans. A wide range of studies therefore becomes feasible. For example, there have been problems associated with metabolic studies of isolated organs such as the brain which are particularly sensitive to hypoxia; these problems can now be circumvented by studying the organ within the whole animal. Theories of metabolic control are based largely on studies performed in vitro, and it should now prove possible to test some of these theories directly in vivo. NMR could provide significant advances in our understanding of organ preservation and function. Finally,31P NMR provides a new method of studying the metabolic basis and effects of disease in humans and in animal models. The technique could also provide an objective way of assessing the effectiveness of procedures that are used in the treatment of human disease.
Seasonal commitment of HMGCoA reductase activity to vitellogenin production
Authors: Manzi Pamela , Bruscalupi Giovannella , Castellano Flavia , Trentalance Anna 
Published: February 2005

In female frogs (Rana Esculenta) during gametogenesis the cholesterol synthesized in the liver by 3-hydroxy-3-methylglutaryl coenzyme A reductase is mostly exported into the blood and taken up by the oocytes.
In order to understand the fate of the neosynthesized cholesterol, female and male frogs and estrogenized male controls were injected with the labelled precursor14C mevalonate.
In females and in estrogenized controls, mevalonate-derived radioactivity is found in a plasmatic lipoprotein that has been identified as vitellogenin by immunological detection.
The increased 3-hydroxy-3-methylglutaryl coenzyme A reductase activity present in females in Fall is likely to be committed to provide cholesterol for the lipidation of this cholesterol-rich protein.