bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experience in the management of patients with HTLV-1 positive adult T-cell leukemia/lymphoma in a Latin American center
Authors: Floyd-Aristizábal Danielle , Barreiro García Cristian Alonso , Arias-Valderrama Oriana , Proaño Millán Catalina , Silva Perez Nhora María , Rosales Martinez Joaquín Donaldo , Arrieta López Elizabeth 
Published: February 2025

Background: Adult T-cell leukemia/lymphoma (ATL) is a neoplasm with a high prevalence in certain regions such as southwestern Japan, the Caribbean, sub-Saharan Africa, South America, and Australia. In Colombia, the seroprevalence of human T-cell lymphotropic virus type 1 (HTLV-1) has been reported in specific populations, but there is limited information about the clinical course and management of ATL in the country. Objectives: To describe the clinical characteristics, treatment patterns, and outcomes of patients diagnosed with HTLV-1-positive ATL in a high-complexity healthcare institution in Colombia and compare these findings with reports from other geographic regions. Design: Observational retrospective cohort study conducted in a single high-complexity healthcare institution in Cali, Colombia, including patients diagnosed between 2011 and January 2022. Methods: This study presents an observational retrospective cohort of patients diagnosed with HTLV-1-positive ATL and managed at a high-complexity institution in Cali, Colombia. Eligible patients were adults diagnosed with ATL and HTLV-1 seropositivity, receiving treatment between 2011 and January 2022. Demographic, clinical, and treatment-related variables were collected and analyzed using descriptive and survival analyses. Results: Thirty-three patients diagnosed with ATL between 2011, and January 2022 were identified and included in the study. Most patients were female (52%) with a median age of 54 years. Acute presentation was the most common (64%), and most patients were identified at Ann Arbor stage 4. Treatment mainly consisted of various chemotherapy protocols, with cyclophosphamide, doxorubicin hydrochloride, vincristine sulfate, and prednisone (CHOP)-like regimens being the most frequently used. However, the overall response rate to chemotherapy was low, and most patients experienced complications and toxicities associated with treatment. Conclusion: This study provides insights into the clinical course and management of ATL in a Colombian population. The findings highlight the predominance of acute presentations, advanced disease stages at diagnosis, and challenges in achieving a complete response with conventional chemotherapy. Further research is needed to improve treatment strategies, identify prognostic markers, and develop more effective therapies for ATL patients in Colombia.
Overcoming barriers and enabling artificial intelligence adoption in allied health clinical practice: A qualitative study
Authors: Hoffman Jane , Wenke Rachel , Angus Rebecca L , Shinners Lucy , Richards Brent , Hattingh Laetitia 
Published: February 2025

Background Artificial intelligence (AI) has the potential to revolutionise healthcare. If the implementation is successful it has the potential to improve healthcare outcomes for patients and organisations. Little is known about the perceptions of allied health professionals (AHPs) towards AI in healthcare. Objective This study investigated barriers and enablers to AI implementation in the delivery of healthcare from the AHPs perspective. Methods Qualitative methodology informed by behaviour change theory using focus groups with AHPs at a health service in Queensland, Australia. Results Twenty-four barriers and 24 enablers were identified by 25 participants across four focus groups. Barriers included: lack of AI knowledge, explainability challenges, risk to professional practice, negative impact on professional practice, and role replacement. Enablers include AI training and education, regulation, reputation, understanding the healthcare benefits of AI and engaging clinical champions. Conclusions AHPs have concerns about the impact and trustworthiness of AI and the readiness of organisations to support its use. Organisations must take a proactive approach and adopt targeted and multifaceted strategies to address barriers. This may include workforce upskilling, clear communication of the benefits of AI use of local champions and ongoing research.
Conversion to Total Knee Arthroplasty After High Tibial Osteotomy: A Systematic Review and Meta-analysis
Authors: Loke Ryan Wai Keong , Chan Yang Kai , Lim Yao Hui , Tan Barry Wei Loong , Hui James Hoi Po 
Published: February 2025

Background: High tibial osteotomy (HTO) is a treatment option for younger, active patients with medial compartment knee osteoarthritis. Clinical results of HTO have been shown to deteriorate over time despite initial satisfactory results, requiring patients to eventually undergo conversion to total knee arthroplasty (TKA). Evidence monitoring survivorship of HTO remains scarce and potentially outdated. Purpose: To investigate the impact of concomitant cartilage repair procedures, conversion to TKA, and associated complications for HTO. Study Design: Systematic review; Level of evidence, 4. Methods: We searched MEDLINE, Embase, Cochrane Library, and SCOPUS from inception to July 18, 2023, for studies reporting on survivorship and associated complications after medial opening-wedge HTO. Pooled analysis of conversion to TKO was categorized as occurring at <5 years, 5 to 10 years, or >10 years postoperatively. Further subgrouping was performed on studies reporting on HTO with concomitant cartilage repair procedures. Results: Overall, 59 studies comprising 5162 patients were included. Rates of conversion to TKA were 4.5% at <5 years, 8.3% at 5 to 10 years, and 11.2% at >10 years. When comparing patients with isolated HTO versus HTO with concomitant cartilage procedures (including mesenchymal stem cell augmentation, osteochondral allograft transplantation, microfracture, abrasion arthroplasty, and autologous chondrocyte implantation), there was no significant difference in survival rates at <5 years (relative risk, 0.78 [95% CI, 0.45-1.33]; P = .36) or 5 to 10 years (relative risk = 0.76 [95% CI, 0.32-1.83]; P = .55). The overall complication rate was 12.1%; intraoperative lateral hinge and tibial plateau fractures had pooled complication rates of 1.6% and 2.0%, respectively. The rate of nonunion was 1.7%, and pooled rates of superficial and deep infections were 2.6% and 2.0% respectively. Conclusion: Rates of conversion to TKA and complications were low and acceptable, although survival rates decreased with time. Concomitant cartilage repair procedures as a whole did not significantly improve survivorship; however, more high-quality studies are warranted to determine the impact of individual concomitant cartilage repair procedures.
Plasma-derived extracellular vesicles prime alveolar macrophages for autophagy and ferroptosis in sepsis-induced acute lung injury
Authors: Ye Rongzong , Wei Yating , Li Jingwen , Zhong Yu , Chen Xiukai , Li Chaoqian 
Published: February 2025

Abstract
Sepsis-induced acute respiratory distress syndrome (ARDS) is a severe complication of sepsis and the leading cause of mortality. Although the role of alveolar macrophages (AMs) in stabilizing pulmonary homeostasis is well established, the effects of circulating extracellular vesicles (EVs) on AMs remain largely unknown. In this study, an investigation was conducted to map the miRNA and protein expression profiles of EVs derived from septic plasma. Notably, EV-based panels (miR-122-5p, miR-125b-5p, miR-223-3p, OLFM4, and LCN2) have been found to be associated with the severity or prognosis of sepsis, with promising AUC values. Moreover, the levels of LCN2, miR-122-5p, and miR-223-3p were identified as independent predictors of septic ARDS. The in vitro coculture results revealed that the effects of LPS-EVs from the plasma of sepsis-induced acute lung injury (ALI), which carry pro-inflammatory EVs, were partly mediated by miR-223-3p, as evidenced by the promotion of inflammation, autophagy and ferroptosis in AMs. Mechanistically, the upregulation of miR-223-3p in LPS-EVs triggers autophagy and ferroptosis in AMs by activating Hippo signaling via the targeting of MEF2C. In vivo, the inhibition of miR-223-3p effectively mitigated LPS-EV-induced inflammation and AM death in the lungs, as well as histological lesions. Overall, miR-223-3p in LPS-EVs contributes to sepsis-induced ALI by priming AMs for autophagy and ferroptosis through the MEF2C/Hippo signaling pathway. These findings suggest a novel mechanism of plasma-AM interaction in sepsis-induced ALI, offering a plausible strategy for assessing septic progression and treating lung injury.
Overview of styles, content, learning effects and attitudes of students towards digitally enhanced physiotherapy education – a scoping review
Authors: Pagels Larissa , Schindler Oskar , Luedtke Kerstin 
Published: February 2025

Abstract

Background
Digital competences are essential for lifelong learning, as highlighted by the European Commission and emphasized in the Digital Education Action Plan 2021–2027. The COVID-19 pandemic necessitated an unprecedented shift to online education, profoundly impacting fields like physiotherapy that heavily rely on practical skills. This scoping review aims to provide an overview of currently applied digitally enhanced learning methods, content, effect on knowledge gain and student perceptions in physiotherapy education.

Methods
Following PRISMA guidelines for scoping reviews, a comprehensive search was conducted across multiple databases, including Medline, Web of Science, and ERIC, incorporating hand searches and expert consultations. Studies were included if they reported on any digitally enhanced educational methods in physiotherapy education, involving qualitative studies, clinical trials, observational studies, or case reports published in English or German from 2010 to February 2024. Data extraction focused on the digital tools that were used, the educational contents, individually measured outcomes, and the impact of digital education.

Results
Out of 2988 screened studies, 67 met the inclusion criteria, encompassing 7160 participants. These sources of evidence primarily used quantitative methods (n = 51), with a minority using qualitative (n = 7) or mixed methods (n = 6). Nearly half employed hybrid educational approaches. Outcome measures included knowledge, performance, perception, satisfaction, and attitudes. Most sources of evidence reported positive impacts of digitally enhanced education, particularly in knowledge transfer and skill performance. Synchronous and asynchronous methods were used, with varying success across theoretical and practical courses. Gamification and virtual reality emerged as promising tools for enhancing engagement and learning outcomes. However, challenges included the limited direct interaction and perceived self-efficacy among students.

Conclusion
Digitally enhanced learning formats in physiotherapy education can enhance learning experiences and is generally welcomed by students, especially when blended with traditional methods. The integration of innovative digital strategies holds promise for the future of physiotherapy training, contingent on comprehensive support and training for educators and students alike.
Prmt5 is essential for intestinal stem cell maintenance and homeostasis
Authors: Yang Li , Li Xuewen , Shi Chenyi , Zhao Bing 
Published: February 2025

Abstract
Intestinal homeostasis relies on the continuous renewal of intestinal stem cells (ISCs), which could be epigenetically regulated. While protein arginine methyltransferase 5 (Prmt5) is known to play a key role in multiple organs as an epigenetic modifier, its specific function in maintaining intestinal homeostasis remains to be elucidated. Here, we show that Prmt5 is highly expressed in mouse crypts. The deletion of Prmt5 results in ISCs deficiency, ectopic localization of Paneth cells, and spontaneous colitis. Mechanistically, Prmt5 sustains a high level of H3K27ac accumulation by inhibiting Hdac9 expression in the intestinal epithelium, and maintains the stemness of ISCs in a cell-autonomous manner. Notably, inhibition of histone deacetylases can rescue both self‐renewal and differentiation capacities of Prmt5‐depleted ISCs. These findings highlight Prmt5 as a critical regulator in intestinal epithelium development and tissue homeostasis.
Enhancing microbial predator–prey detection with network and trait-based analyses
Authors: Martínez Rendón Cristina , Braun Christina , Kappelsberger Maria , Boy Jens , Casanova-Katny Angélica , Glaser Karin , Dumack Kenneth 
Published: February 2025

Abstract

Background
Network analyses are often applied to microbial communities using sequencing survey datasets. However, associations in such networks do not necessarily indicate actual biotic interactions, and even if they do, the nature of the interactions commonly remains unclear. While network analyses are valuable for generating hypotheses, the inferred hypotheses are rarely experimentally confirmed.

Results
We employed cross-kingdom network analyses, applied trait-based functions to the microorganisms, and subsequently experimentally investigated the found putative predator–prey interactions to evaluate whether, and to what extent, correlations indicate actual predator–prey relationships. For this, we investigated algae and their protistan predators in biocrusts of three distinct polar regions, i.e., Svalbard, the Antarctic Peninsula, and Continental Antarctica. Network analyses using FlashWeave indicated that 89, 138, and 51 correlations occurred between predatory protists and algae, respectively. However, trait assignment revealed that only 4.7–9.3% of said correlations link predators to actually suitable prey. We further confirmed these results with HMSC modeling, which resulted in similar numbers of 7.5% and 4.8% linking predators to suitable prey for full co-occurrence and abundance models, respectively. The combination of network analyses and trait assignment increased confidence in the prediction of predator–prey interactions, as we show that 82% of all experimentally investigated correlations could be verified. Furthermore, we found that more vicious predators, i.e., predators with the highest growth rate in co-culture with their prey, exhibit higher stress and betweenness centrality — giving rise to the future possibility of determining important predators from their network statistics.

Conclusions
Our results support the idea of using network analyses for inferring predator–prey interactions, but at the same time call for cautionary consideration of the results, by combining them with trait-based approaches to increase confidence in the prediction of biological interactions.
Quantitative profiling of the vaginal microbiota improves resolution of the microbiota-immune axis
Authors: Armstrong Eric , Liu Rachel , Pollock James , Huibner Sanja , Udayakumar Suji , Irungu Erastus , Ngurukiri Pauline , Muthoga Peter , Adhiambo Wendy , Yegorov Sergey , Kimani Joshua , Beattie Tara , Coburn Bryan , Kaul Rupert 
Published: February 2025

Abstract

Background
The composition of the vaginal microbiota is closely linked to adverse sexual and reproductive health outcomes, due in part to effects on genital immunology. Compositional approaches such as metagenomic sequencing provide a snapshot of all bacteria in a sample and have become the standard for characterizing the vaginal microbiota, but only provide microbial relative abundances. We hypothesized that the addition of absolute abundance data would provide a more complete picture of host-microbe interactions in the female genital tract.

Results
We analyzed cervicovaginal secretions from 196 female sex workers in Kenya and found that bacterial load was elevated among women with diverse, bacterial vaginosis (BV)-type microbiota and lower among women with Lactobacillus predominance. Bacterial load was also positively associated with proinflammatory cytokines, such as IL-1α, and negatively associated with chemokines, such as IP-10. The associations between bacterial load and immune factors differed across bacterial community states, but L. crispatus predominance was the only microbial community where higher bacterial load was not associated with higher proinflammatory cytokines. Total vaginal bacterial load was also a stronger predictor of the genital immune environment than BV by Nugent score, the current clinical standard, in the Kenya-based cohort and in a Uganda-based confirmatory cohort.

Conclusions
Our results suggest that total vaginal bacterial load is at least as strong a predictor of the genital immune milieu as current BV clinical diagnostic tools, supporting exploration of the vaginal bacterial load as a predictor of adverse reproductive and sexual health outcomes.
Association between the triglyceride–glucose index and the risk of acute kidney injury in critically ill patients with acute pancreatitis: a retrospective study
Authors: Wang Zheng , Zhang Haoyu , Xie Xiaozhou , Li Jie , Jia Yuchen , Lu Jiongdi , Gao Chongchong , Cao Feng , Li Fei 
Published: February 2025

Abstract

Background
The triglyceride–glucose (TyG) index is increasingly recognized for its ability to predict cardiovascular and metabolic risks. This study investigated the correlation between the TyG index and the risk of acute kidney injury(AKI) in critical ill patients with acute pancreatitis(AP).

Methods
The Medical Information Mart for Intensive Care IV database was retrospectively searched to identify AP patients hospitalized in the intensive care unit. The primary outcome measure was the incidence of AKI. The secondary endpoint was in-hospital mortality and the rate of renal replacement therapy(RRT) use. Cox regression analysis and restricted cubic spline were used to analyze TyG index association with AKI risk. Kaplan–Meier survival analysis was performed to assess the incidence of endpoints in the different groups.

Results
A total of 848 patients were enrolled. The incidence of AKI was 61.56%.The in-hospital mortality was 11.69%. Kaplan–Meier analysis showed that the TyG index ≥ 8.78 group has a high incidence of AKI and high risk of requiring RRT (P < 0.001). Multivariable Cox regression analysis showed whether TyG index was a continuous variable (HR, 1.65 [95% CI 1.10–2.48], P = 0.015) or a categorical variable (HR, 1.72 [95% CI 1.09–2.79], P = 0.028), and the TyG index was independently associated with the risk of AKI in AP patients. The restricted cubic splines model illustrated the linear relationship between higher TyG index and increased risk of AKI in this specific patient population.

Conclusions
High TyG index is an independent risk factor for AKI in critical ill patients with AP. Assessing the TyG index may be beneficial for early stratification and interventions to improve prognosis.
Towards high-quality informatics K-12 education in Europe: key insights from the literature
Authors: Sampson Demetrios , Kampylis Panagiotis , Moreno-León Jesús , Bocconi Stefania 
Published: February 2025

Abstract
This paper explores the evolving landscape of informatics education in European primary and secondary schools, analysing academic and grey literature to define the state of play and open questions related to ‘high-quality informatics education’. It underlines the strategic importance of promoting high-quality informatics education to prepare students for life and work in the digital era, contributing to European societies and economies’ social and economic resilience. Drawing on a review of over 180 recent academic publications, policy documents, and grey literature, it provides an overview of how informatics education is being implemented across Europe and beyond, highlighting recent curricular developments, pedagogical practices, and policy initiatives. The paper also identifies and analyses key open issues related to high-quality informatics education, organised into four clusters: student-related (e.g., equity and inclusion), teacher-related (e.g., professional development, shortage of qualified teachers), school-related (e.g., the need for whole-school approach) and curriculum- and resource-related (e.g., competing curriculum priorities, quality of teaching and learning materials). Finally, the paper offers recommendations for policymakers, researchers, and practitioners (school leaders and educators) related to the key open issues of high-quality K-12 informatics education. Overall, the paper contributes to the discussion on high-quality informatics K-12 education in Europe towards identifying and addressing major challenges for equitable access to quality informatics education for all European K-12 students.
Unveiling the effects of post-monsoon agricultural biomass burning on aerosols, clouds, and radiation in Northwest India
Authors: Khatri Pradeep , Hayasaka Tadahiro , Patra Prabir K. , Letu Husi , Jethva Hiren , Hayashida Sachiko 
Published: February 2025

Abstract
The post-monsoon agricultural biomass burning activities in Northwest India have been recognized as a significant socio-environmental problem in recent years, primarily due to their severe impacts on air quality degradation across a wide area, including the capital New Delhi. Although these biomass burning activities have been extensively studied from an air quality perspective, their potential impacts on the climate system, particularly through their influences on cloud and radiation fields, have been largely overlooked. In this study, we aim to address this research gap by analyzing fire, meteorological parameters, aerosol, cloud, and radiation data spanning nearly two decades (2002–2021), obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS) aboard the Aqua satellite, Modern-Era Retrospective Analysis Research and Applications, Version 2 (MERRA-2), and the Fifth Generation of European Centre for Medium-Range Weather Forecasts (ECMWF) Reanalysis (ERA5). Our analysis reveals a notable increase in agricultural biomass burning intensity in Northwest India over the past two decades, contributing significantly to air quality degradation. Our analysis further indicates a delay in peak burning time (day of the year) and a shortening of the period of intense burning, reflecting changes in farming practices and agricultural biomass burning in Northwest India over the past two decades. These agricultural biomass burning activities substantially elevate total and light-absorbing aerosols, thereby affecting cloud properties and altering the radiation budget. The intensification of these burning activities can cause an increase in cloud droplet size and a decrease in cloud optical thickness, suggesting an enhancement of the cloud droplet collision-coalescence process during the period of intense burning. Similarly, the intensification of burning activities leads to increased cooling effects at the surface and top-of-the-atmosphere across shortwave and longwave spectral ranges, while inducing a heating effect within the atmosphere. These findings highlight the potential impacts of agricultural biomass burning activities on the regional climate system and hydrological cycle, emphasizing the need for more detailed studies in the future.