bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow Diversion for the Treatment of Posterior Inferior Cerebellar Aneurysms: A Novel Classification of Posterior Inferior Cerebellar Artery Origin
Authors: Sathya Anvitha , Enriquez-Marulanda Alejandro , Young Michael , Shutran Max , Taussky Phillip , Ogilvy Christopher S. 
Published: December 2023

BACKGROUND AND OBJECTIVES:
Flow diversion (FD) for posterior circulation aneurysms, such as proximal posterior inferior cerebellar artery (PICA) aneurysms, remains “off-label” and controversial. Although there are reports of using FD in the vertebral artery (VA) to treat PICA aneurysms, the differences between structural PICA-origin variants have not been explored. We analyzed PICA aneurysms treated with FD, assessed radiographic and clinical outcomes, and presented a novel classification of the PICA origin in relationship to aneurysm anatomy.

METHODS:
We performed a retrospective study of prospective data for intracranial aneurysms treated with FD at a major academic institution from 2013 to 2022. Proximal PICA aneurysms that underwent FD placement in the V4 segment of the vertebral artery were included for analysis. A literature review was performed on PubMed to evaluate previously published cases. The PICA origin was characterized by 4 distinct subtypes. Type 1 describes the PICA originating adjacent/separate to the aneurysm neck, Type 2 with the PICA originating from the aneurysm neck, Type 3 with the PICA originating from the aneurysm dome, and Type 4 (True PICA aneurysm) with the aneurysm located proximally on PICA, distal to the PICA-VA junction.

RESULTS:
Thirteen proximal PICA aneurysms were identified and included in the analysis. Patients were primarily female (76.9%), with a median age of 62 years. The aneurysm median maximum diameter was 5.8 mm. From the total sample (institutional and literature review cases), type 1 had a 100% complete and near-complete occlusion rate, type 2 had 75.0%, type 3 had 88.9%, and type 4 had 75%. The overall complete and near-complete occlusion rate was 83.3% (20/24).

CONCLUSION:
FD in the V4 VA segment is an effective way to treat proximal PICA aneurysms. Exploring the relationship between PICA origin is a helpful method in predicting occlusion rates for proximal PICA aneurysms and may lead to improved treatment considerations.
The H2Bub1-deposition complex is required for human and mouse cardiogenesis
Authors: Barish Syndi , Berg Kathryn , Drozd Jeffrey , Berglund-Brown Isabella , Khizir Labeeqa , Wasson Lauren K. , Seidman Christine E. , Seidman Jonathan G. , Chen Sidi , Brueckner Martina 
Published: December 2023

ABSTRACT
De novo variants affecting monoubiquitylation of histone H2B (H2Bub1) are enriched in human congenital heart disease. H2Bub1 is required in stem cell differentiation, cilia function, post-natal cardiomyocyte maturation and transcriptional elongation. However, how H2Bub1 affects cardiogenesis is unknown. We show that the H2Bub1-deposition complex (RNF20-RNF40-UBE2B) is required for mouse cardiogenesis and for differentiation of human iPSCs into cardiomyocytes. Mice with cardiac-specific Rnf20 deletion are embryonic lethal and have abnormal myocardium. We then analyzed H2Bub1 marks during differentiation of human iPSCs into cardiomyocytes. H2Bub1 is erased from most genes at the transition from cardiac mesoderm to cardiac progenitor cells but is preserved on a subset of long cardiac-specific genes. When H2Bub1 is reduced in iPSC-derived cardiomyocytes, long cardiac-specific genes have fewer full-length transcripts. This correlates with H2Bub1 accumulation near the center of these genes. H2Bub1 accumulation near the center of tissue-specific genes was also observed in embryonic fibroblasts and fetal osteoblasts. In summary, we show that normal H2Bub1 distribution is required for cardiogenesis and cardiomyocyte differentiation, and suggest that H2Bub1 regulates tissue-specific gene expression by increasing the amount of full-length transcripts.
Membrane transporters in cell physiology, cancer metabolism and drug response
Authors: Alam Sara , Doherty Emily , Ortega-Prieto Paula , Arizanova Julia , Fets Louise 
Published: December 2023

ABSTRACT
By controlling the passage of small molecules across lipid bilayers, membrane transporters influence not only the uptake and efflux of nutrients, but also the metabolic state of the cell. With more than 450 members, the Solute Carriers (SLCs) are the largest transporter super-family, clustering into families with different substrate specificities and regulatory properties. Cells of different types are, therefore, able to tailor their transporter expression signatures depending on their metabolic requirements, and the physiological importance of these proteins is illustrated by their mis-regulation in a number of disease states. In cancer, transporter expression is heterogeneous, and the SLC family has been shown to facilitate the accumulation of biomass, influence redox homeostasis, and also mediate metabolic crosstalk with other cell types within the tumour microenvironment. This Review explores the roles of membrane transporters in physiological and malignant settings, and how these roles can affect drug response, through either indirect modulation of sensitivity or the direct transport of small-molecule therapeutic compounds into cells.
Optimisation of Multiple Response Processes Using Different Modeling Techniques
Authors: Maciel Gomes Fabrício , Imamura Célia Sayuri , De Souza Sampaio Nilo Antonio , Monteiro Pereira Félix , De Souza Andrade Herlandi , Borges Silva Messias 
Published: December 2023

Purpose: This article aims to compare the impact on process optimisation with multiple responses of two different mathematical modelling methods: Ordinary Least Squares Method (OLS) and Symbolic Regression Method (SR).
Methodology/Approach: Data from the literature were selected from the design of experiments for a process with multiple responses. Using these data, models were obtained that represented each response as a function of independent variables using the OLS and SR techniques. Then, the Desirability method was applied together with the Generalized Reduced Gradient (GRG) in order to obtain the process adjustment that would lead to the optimisation of the responses.
Findings: The findings illustrate that the SR modelling technique yields models with superior predictive capabilities when contrasted with the OLS technique. Throughout the optimisation process, it becomes evident that the adjustments in the process diverge, even though the desirability function's value exhibits negligible variation.
Research Limitation/implication: This research considered only an SR algorithm and a process with two dependent variables and two independent variables.
Originality/Value of paper: No works were found in the literature that reported the use of the Age-Layered Population Structure (ALPS) algorithm in modelling processes that contain multiple responses. Furthermore, no comparison of this method with the OLS method was available.
Category: Research paper.
Malposition is main cause of failure of Oxford mobile-bearing medial unicompartmental knee arthroplasty
Authors: Sang Weilin , Qiu Hongjiu , Xu Ying , Pan Yuqin , Ma Jinzhong , Zhu Libo 
Published: December 2023

AimsUnicompartmental knee arthroplasty (UKA) is the preferred treatment for anterior medial knee osteoarthritis (OA) owing to the rapid postoperative recovery. However, the risk factors for UKA failure remain controversial.MethodsThe clinical data of Oxford mobile-bearing UKAs performed between 2011 and 2017 with a minimum follow-up of five years were retrospectively analyzed. Demographic, surgical, and follow-up data were collected. The Cox proportional hazards model was used to identify the risk factors that contribute to UKA failure. Kaplan-Meier survival was used to compare the effect of the prosthesis position on UKA survival.ResultsA total of 407 patients who underwent UKA were included in the study. The mean age of patients was 61.8 years, and the mean follow-up period of the patients was 91.7 months. The mean Knee Society Score (KSS) preoperatively and at the last follow-up were 64.2 and 89.7, respectively (p = 0.001). Overall, 28 patients (6.9%) with UKA underwent revision due to prosthesis loosening (16 patients), dislocation (eight patients), and persistent pain (four patients). Cox proportional hazards model analysis identified malposition of the prostheses as a high-risk factor for UKA failure (p = 0.007). Kaplan-Meier analysis revealed that the five-year survival rate of the group with malposition was 85.1%, which was significantly lower than that of the group with normal position (96.2%; p < 0.001).ConclusionUKA constitutes an effective method for treating anteromedial knee OA, with an excellent five-year survival rate. Aseptic loosening caused by prosthesis malposition was identified as the main cause of UKA failure. Surgeons should pay close attention to prevent the potential occurrence of this problem.Cite this article: Bone Jt Open 2023;4(12):914–922.
Can antibody conjugated nanomicelles alter the prospect of antibody targeted therapy against schistosomiasis mansoni?
Authors: Amer Eglal I. , Allam Sonia R. , Hassan Aceel Y. , El-Fakharany Esmail M. , Agwa Mona M. , Khattab Sherine N. , Sheta Eman , El-Faham Marwa H. 
Published: December 2023

Background
CLA (conjugated linoleic acid)-mediated activation of the schistosome tegument-associated sphingomyelinase and consequent disruption of the outer membrane might allow host antibodies to access the apical membrane antigens. Here, we investigated a novel approach to enhance specific antibody delivery to concealed surface membrane antigens of Schistosoma mansoni utilising antibody-conjugated-CLA nanomicelle technology.

Methodology/Principal findings
We invented and characterised an amphiphilic CLA-loaded whey protein co-polymer (CLA-W) as an IV injectable protein nanocarrier. Rabbit anti-Schistosoma mansoni infection (anti-SmI) and anti-Schistosoma mansoni alkaline phosphatase specific IgG antibodies were purified from rabbit sera and conjugated to the surface of CLA-W co-polymer to form antibody-conjugated-CLA-W nanomicelles (Ab-CLA-W). We investigated the schistosomicidal effects of CLA-W and Ab-CLA-W in a mouse model of Schistosoma mansoni against early and late stages of infection. Results showed that conjugation of nanomicelles with antibodies, namely anti-SmI, significantly enhanced the micelles’ schistosomicidal and anti-pathology activities at both the schistosomula and adult worm stages of the infection resulting in 64.6%-89.9% reductions in worm number; 72.5–94% and 66.4–85.2% reductions in hepatic eggs and granulomas, respectively. Treatment induced overall improvement in liver histopathology, reducing granuloma size and fibrosis and significantly affecting egg viability. Indirect immunofluorescence confirmed CLA-W-mediated antigen exposure on the worm surface. Electron microscopy revealed extensive ultrastructural damage in worm tegument induced by anti-SmI-CLA-W.

Conclusion/Significance
The novel antibody-targeted nano-sized CLA delivery system offers great promise for treatment of Schistosoma mansoni infection and control of its transmission. Our in vivo observations confirm an immune-mediated enhanced effect of the schistosomicidal action of CLA and hints at the prospect of nanotechnology-based immunotherapy, not only for schistosomiasis, but also for other parasitic infections in which chemotherapy has been shown to be immune-dependent. The results propose that the immunodominant reactivity of the anti-SmI serum, Schistosoma mansoni fructose biphosphate aldolase, SmFBPA, merits serious attention as a therapeutic and vaccine candidate.
A medium-chain fatty acid analogue prevents hepatosteatosis and decreases inflammatory lipid metabolites in a murine model of parenteral nutrition-induced hepatosteatosis
Authors: Cho Bennet S. , Fligor Scott C. , Fell Gillian L. , Secor Jordan D. , Tsikis Savas T. , Pan Amy , Yu Lumeng J. , Ko Victoria H. , Dao Duy T. , Anez-Bustillos Lorenzo , Hirsch Thomas I. , Lund Jenny , Rustan Arild C. , Fraser David A. , Gura Kathleen M. , Puder Mark 
Published: December 2023

Background
Parenteral (intravenous) nutrition is lifesaving for patients with intestinal failure, but long-term use of parenteral nutrition often leads to liver disease. SEFA-6179 is a synthetic medium-chain fatty acid analogue designed to target multiple fatty acid receptors regulating metabolic and inflammatory pathways. We hypothesized that SEFA-6179 would prevent hepatosteatosis and lipotoxicity in a murine model of parenteral nutrition-induced hepatosteatosis.

Methods
Two in vivo experiments were conducted. In the first experiment, six-week-old male mice were provided an ad lib fat-free high carbohydrate diet (HCD) for 19 days with orogastric gavage of either fish oil, medium-chain triglycerides, or SEFA-6179 at a low (0.3mmol/kg) or high dose (0.6mmol/kg). In the second experiment, six-week-old mice were provided an ad lib fat-free high carbohydrate diet for 19 days with every other day tail vein injection of saline, soybean oil lipid emulsion, or fish oil lipid emulsion. Mice then received every other day orogastric gavage of medium-chain triglyceride vehicle or SEFA-6179 (0.6mmol/kg). Hepatosteatosis was assessed by a blinded pathologist using an established rodent steatosis score. Hepatic lipid metabolites were assessed using ultra-high-performance liquid chromatography-mass spectrometry. Effects of SEFA-6179 on fatty acid oxidation, lipogenesis, and fatty acid uptake in human liver cells were assessed in vitro.

Results
In the first experiment, mice receiving the HCD with either saline or medium-chain triglyceride treatment developed macrovesicular steatosis, while mice receiving fish oil or SEFA-6179 retained normal liver histology. In the second experiment, mice receiving a high carbohydrate diet with intravenous saline or soybean oil lipid emulsion, along with medium chain triglyceride vehicle treatment, developed macrovescular steatosis. Treatment with SEFA-6179 prevented steatosis. In each experiment, SEFA-6179 treatment decreased arachidonic acid metabolites as well as key molecules (diacylglycerol, ceramides) involved in lipotoxicity. SEFA-6179 increased both β- and complete fatty oxidation in human liver cells, while having no impact on lipogenesis or fatty acid uptake.

Conclusions
SEFA-6179 treatment prevented hepatosteatosis and decreased toxic lipid metabolites in a murine model of parenteral nutrition-induced hepatosteatosis. An increase in both β- and complete hepatic fatty acid oxidation may underlie the reduction in steatosis.
Construction of a 5-gene prognostic signature based on oxidative stress related genes for predicting prognosis in osteosarcoma
Authors: Hong Xiaofang , Fu Ribin 
Published: December 2023

Background
The understanding of the complex biological scenario of osteosarcoma will open the way to identifying new strategies for its treatment. Oxidative stress is a cancer-related biological scenario. At present, it is not clear the oxidative stress genes in affecting the prognosis and progression of osteosarcoma, the underlying mechanism as well as their impact on the classification of osteosarcoma subtypes.

Methods
We selected samples and sequencing data from TARGET data set and GSE21257 data set, and downloaded oxidative stress related-genes (OSRGs) from MsigDB. Univariate Cox analysis of OSRG was conducted using TARGET data, and the prognostic OSRG was screened to conduct unsupervised clustering analysis to identify the molecular subtypes of osteosarcoma. Through least absolute shrinkage and selection operator (LASSO) regression analysis and COX regression analysis of differentially expressed genes (DEGs) between subgroups, a risk assessment system for osteosarcoma was developed.

Results
45 prognosis-related OSRGs genes were acquired, and two molecular subtypes of osteosarcoma were clustered. C2 cluster displayed prolonged overall survival (OS) accompanied with high degree of immune infiltration and enriched immune pathways. While cell cycle related pathways were enriched in C2 cluster. Based on DEGs between subgroups and Lasso analysis, 5 hub genes (ZYX, GJA5, GAL, GRAMD1B, and CKMT2) were screened to establish a robust prognostic risk model independent of clinicopathological features. High-risk group had more patients with cancer metastasis and death as well as C1 subtype with poor prognosis. Low-risk group exhibited favorable OS and high immune infiltration status. Additionally, the risk assessment system was optimized by building decision tree and nomogram.

Conclusions
This study defined two molecular subtypes of osteosarcoma with different prognosis and tumor immune microenvironment status based on the expression of OSRGs, and provided a new risk assessment system for the prognosis of osteosarcoma.
How could mouthwashes affect the color stability and translucency of various types of monolithic zirconia? An in-vitro study
Authors: Giti Rashin , Jebal Reza 
Published: December 2023

Objectives
This study aimed to evaluate the color stability and translucency of various types of monolithic zirconia after immersion in chlorhexidine and Listerine mouthwashes.

Materials and methods
This experimental study was performed on 36 disk-shaped specimens fabricated from low-translucent, high-translucent, and multilayered monolithic zirconia (n = 12 per group). Each group was equally divided and immersed in either 2% chlorhexidine (CHX) or Listerine mouthwash for 2 min daily over 7 days. Changes in color (ΔE) and the translucency parameter (ΔTP) were evaluated and compared. The data were analyzed with one-way ANOVA followed by Tukey’s post-hoc tests (α = 0.05).

Results
Chlorhexidine caused a significantly lower ΔE and a significantly higher ΔTP in multilayered zirconia compared to the low-translucency (ΔE: P = 0.0027, ΔTP: P<0.001) and the high-translucency zirconia group (ΔE: P<0.001, ΔTP: P = 0.022). Listerine caused a significantly higher ΔE in the high-translucency zirconia group compared to the multilayered zirconia group (P = 0.0165). It also resulted in a significantly higher mean ΔTP in the multilayered zirconia group compared to the low-translucency (P = 0.0003) and high-translucency zirconia groups (P = 0.019).

Conclusions
In both mouthwashes, multilayered monolithic zirconia exhibited the highest color stability among the tested materials; albeit with the most pronounced changes in translucency. Meanwhile, high-translucency monolithic zirconia was more prone to discoloration when exposed to both mouthwashes.
Pensar la autonomía femenina en el contexto de las políticas públicas locales. El caso de la Municipalidad de Santa Fe (2011-2019)
Authors: Molina Adriana 
Published: December 2023

Los gobiernos locales han ganado relevancia en las últimas décadas. Nuevas demandas han aparecido y los municipios han tenido que dar respuesta a una sociedad que cada vez necesita más. En este sentido, dentro de esas nuevas demandas, las cuestiones de género han ganado terreno.
El presente trabajo busca dar cuenta de las maneras en que desde la gestión pública se generaron acciones para dar respuesta a situaciones concretas pensadas desde una perspectiva de derechos humanos, pero sobre todo desde una perspectiva de género; teniendo como eje de análisis el concepto de autonomía feminista.
Ante ello, el objetivo del presente artículo será analizar el concepto de autonomía feminista en las políticas públicas con perspectiva de género llevadas adelante por la Municipalidad de Santa Fe en el período comprendido entre 2011 y 2019.
Del árbol sagrado a la “ceguera de las plantas”
Authors: Drivet Leandro 
Published: December 2023

La deforestación se ha convertido en una invariante en el modo de producción/destrucción dominante. Esto constituye un problema mayúsculo. Pese a que las medidas orientadas a ponerle límite están aumentando, aún son insuficientes para detener el patrón destructivo que obedece a una clara racionalidad económica. En este trabajo interrogamos algunas de las razones de la tolerancia ante este aspecto del ecocidio. Sin desconocer el cinismo crematístico de las élites, postulamos que el desinterés mayoritario que posibilita la destrucción de la biodiversidad tiene fundamentos profundos en la ignorancia y en los prejuicios erróneos. De aquí que conjeturemos que una transformación del vínculo destructivo de nuestra cultura con la naturaleza requiere de una revisión de los sentidos de largo plazo que le dieron forma. En particular aquí, nos interesa proponer una reflexión de carácter filosófico enfocada en la patología llamada “ceguera vegetal”: nuestra incapacidad para advertir la presencia y la relevancia de la vida vegetal. Para ilustrar el pasaje del estatuto sagrado al estatuto profano de las plantas, proponemos una interpretación laica de dos mitos que evocan la presencia de árboles sagrados. Luego, conectamos este saber con el análisis crítico de la indiferencia cómplice ante el destino sacrificial de los vegetales en la modernidad. Para finalizar, nos preguntamos si el Derecho participa del sesgo denunciado y, bajo el paradigma de la justicia ecológica, sugerimos invertir esfuerzos interdisciplinarios dirigidos a remediar la ceguera de las plantas junto a sus catastróficas consecuencias.
Las caminatas eco-sonoras
Authors: Espinosa Susana 
Published: December 2023

El arte sonoro de la actualidad supera problemáticas epistémicas que devienen de la música acústica, así como de la electroacústica, la música ecológica y la audiovisual. La hibridez y transversalidad de los lenguajes artísticos integrados, así como la fusión entre ciencia, arte y tecnología, desafían al receptor a ampliar su escucha y sensibilizar su percepción hacia todos los sonidos, sean ellos musicales o del medioambiente natural, industrial o social.
Así es que surge la Ecología Acústica como nueva ciencia que estudia la relación de los seres vivos con su medioambiente sónico y se ocupa de la preservación y defensa de ello, por lo que postula un concepto de validez ecológica: el sonido del entorno es un bien que sirve al sujeto que lo recibe.
Para valorar y experimentar estas concepciones, desde el campo de la educación, diversos investigadores proponen la realización de Caminatas Eco–sonoras como un camino creativo y rico para educar al oído y contribuir a la construcción de un nuevo paisaje sonoro que supere la contaminación acústica que sufre la sociedad actual, especialmente en los ámbitos urbanos.
Se realizan en forma grupal con un guía orientador que organiza los tiempos pre–fijados para cada etapa de escucha silenciosa, luego se construyen espacios de reflexión de los sonidos escuchados para analizar sus tipomorfologías características y por último se proponen actividades de intervención sonora a través de la realización de «obras» musicales con los elementos encontrados en el medio ambiente natural.
Entre sus principales objetivos se destacan el concientizar acerca del ruido nocivo para la salud auditiva; el aprender a escuchar con atención y no solo a oír; el integrar todo tipo de sonido en cualquier producción musical; el abrazar la cultura del silencio y del habla a media voz como forma de convivencia sana entre los hombres.