bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can temperate forests deliver both future wood demand and climate-change mitigation?
Authors: Forster Eilidh , Healey John , Styles David 
Published: December 2023

Abstract
Global wood demand is projected to rise but supply capacity is questioned due to limited global forest resources. Furthermore, the lifecycle global warming potential (GWP) impact of additional wood supply and use is poorly understood. For the case of a temperate country, combining forest carbon modelling and life-cycle assessment we show that sustained afforestation to double forest area alongside enhanced productivity can meet lower-bound wood demand projections from 2058. Thus, temperate forestry value-chains can achieve a cumulative GWP benefit of up to 265 Tg CO2-equivalent (CO2e) by 2100 for each 100,000 ha (expanding to 200,000 ha through afforestation) of forest. Net GWP balance depends on which overseas forests supply domestic shortfalls, how wood is used, and rate of industrial decarbonisation. There is considerable but constrained potential for increased wood-use to deliver future climate-change mitigation, providing it is connected with a long-term planting strategy, enhanced tree productivity and efficient wood use.
Evaluation of Adaptability and Yield Performance of Onion (Allium cepa L.) Varieties at Efa Galano, Sayo District of Kellam Wallaga Zone, Western Ethiopia.
Authors: Tesfa Garedo , Olana Gemechisa , Kitila Chala 
Published: December 2023

Abstract
For the study, the research field was laid out and reproduced three times using a randomized complete block design (RCBD). Thus, analysis of variance revealed that the tested onion types had a substantial influence on practically all quantitative parameters except average stand count at harvest, which was non-significant. In the current study, however, the greatest days to maturity (late maturing) were recorded for both Red Bombey and Red Croel (140.333days to mature), whereas the minimum days to maturity (early maturing) was recorded for Adama Red (115.333days to mature). Furthermore, the greatest (6.513t/ha) and least (4.381t/ha) marketable bulb weights were recorded from Red Croel and Adama Red, respectively, while the minimum bulb yield (4.827t/ha) and maximum bulb yield (6.699t/ha) were reported from Red Croel and Adama Red, respectively. Based on the current investigation, it was unable to recommend one variety over another because a single yield trial cannot offer trustworthy information on yield performance of varieties, necessitating a multi-year multi-location yield experiment. However, for the immediate needs of onion crop growers, this experiment suggests that the Red Croel variety was a better bulb yielder than the other analyzed onion varieties in the study area and areas with similar agro-ecological characteristics.
An assessment model for efficacy of patients with relapsed or refractory diffuse large B-cell lymphoma following autologous CD19 chimeric antigen receptor T-cell therapy.
Authors: Xue Bin , Luo Xiu , Liu Yifan , Ye Shiguang , Zhou Lili , Lu Yan , Fu Jianfei , Qian Wenbin , Wang Li , Li Ping , Liang Aibin 
Published: December 2023

Abstract
Background
Currently, there is no predictive model for the efficacy of autologous CD19 chimeric antigen receptor T-cell therapy (CAR-T) in relapsed or refractory diffuse large B-cell lymphoma (r/r DLBCL). This study aims to construct a comprehensive model that takes into account numerous influencing factors to predict the efficacy of CD19 CAR-T therapy.
Methods
A total of 80 r/r DLBCL patients receiving CD19 CAR-T therapy from two centers were included in the study. Multivariable logistic regression analysis model was constructed using data from CAR-T clinical trials as the derivation cohort and real-world data as the validation cohort.
Results
The model was optimized based on the results of clinical practice and further developed into an index model, which demonstrated excellent predictive utility in both the derivation cohort (C-index = 0.891) and the external validation cohort (C-index = 0.797). Calibration curve, decision curve analysis, and clinical impact curve confirmed the clinical utility of the predictive model. The risk stratification based on the index model can indicate differences in progression-free survival (PFS) and overall survival (OS).
Conclusions
the established predictive model for assessing the efficacy of CD19 CAR-T treatment in r/r DLBCL is accurate and clinically useful.
Unveiling Harmful Phase Transition of Kidney Stones and its Clinical Impact
Authors: Maruyama Mihoko , Michibata Uta , Tanaka Yutaro , Yoshimura Masashi , Yoshikawa Hiroshi , Takano Kazufumi , Furukawa Yoshihiro , Momma Koichi , Tajiri Rie , Taguchi Kazumi , Hamamoto Shuzo , Okada Atsushi , Kohri Kenjiro , Yasui Takahiro , Usami Shigeyoshi , Imanishi Masayuki , Mori Yusuke 
Published: December 2023

Abstract
Calcium oxalate kidney stones, the most prevalent type of kidney stones, undergo a multi-step process of crystal nucleation, growth, aggregation, and secondary transition. The secondary transition has been rather overlooked, and thus, the effects on the disease and the underlying mechanism remain unclear. Here, we show, by periodic micro-CT images of human kidney stones in an ex vivo incubation experiment, that the growth of porous aggregates of calcium oxalate dihydrate (COD) crystals triggers the hardening of the kidney stones that causes difficulty in lithotripsy of kidney stone disease in the secondary transition. This hardening was caused by the internal nucleation and growth of precise calcium oxalate monohydrate (COM) crystals from isolated urine in which the calcium oxalate concentrations decreased by the growth of COD in closed grain boundaries of COD aggregate kidney stones. Reducing the calcium oxalate concentrations in urine is regarded as a typical approach for avoiding the recurrence. However, our results revealed that the decrease of the concentrations in closed microenvironments conversely promotes the transition of the COD aggregates into hard COM aggregates. We anticipate that the suppression of the secondary transition has the potential to manage the deterioration of kidney stone disease.
Cold atmospheric plasma activated media selectively affects human head and neck cancer cell lines
Authors: Giacomo Viviana di , Balaha Marwa , Pinti Morena , Marcantonio Maria Carmela Di , Cela Ilaria , Acharya Tirtha Raj , Kaushik Nagendra Kumar , Choi Eun Ha , Rapino Monica , Mazzone Mariangela , Mincione Gabriella , Sala Gianluca , Perrucci Miryam , Locatelli Marcello , Perrotti Vittoria 
Published: December 2023

Abstract
Objective: Cold atmospheric plasma (CAP) is a novel therapeutic approach for cancer treatment. It can be used to treat liquids - plasma-activated media (PAM) - which are then transferred to the target as an exogenous source of reactive oxygen and nitrogen species (RONS). The present study aimed at chemically characterizing different PAM and assessing their in vitro selectivity against head and neck cancer cell lines (HNC).
Materials and methods: PAM were obtained by exposing 2 and 5 mL of medium to CAP for 5, 10 and 20 minutes at a 6 mm working distance. Anions kinetics was evaluated by ion chromatography. In addition, inhibition of cell proliferation by MTS assay, apoptosis occurrence and cell cycle modifications by flow cytometry were assessed on primary human gingival fibroblasts (hGF) and the HNC cell lines HSC2, HSC4 and A253.
Results: All the 2 mL conditions showed a significant reduction in cell proliferation whereas for the 5 mL the effect was milder, but the time-dependence was more evident. In addition, hGF were unaffected by the 5 mL PAM, indicating a selectivity for cancer cells.
Conclusions: The media chemical composition modified by CAP exposure influenced cell proliferation by modulating cell cycle and inducing apoptosis in cancer cells, without affecting normal cells.
Clinical Relevance: The present investigation represents a starting point to favour the clinical translation of CAP as a precision medicine tool by proposing an innovative method, namely ion chromatography, to standardize the quantification of plasma-derived RONS and proving its selectivity in inactivating tumor cells over non-malignant cells. These strategies could be applied to identify the optimal parameter configuration to achieve the desired treatment/therapeutic outcome and to aid the definition of clinical protocols.
NeuroD1 and Ascl1 Convert Human Glial Cells into Neurons in Ex Vivo Culture of Human Brain Tissue
Authors: Xu Liang , Wang Qingsong , Liao Jiancheng , Zheng Jiajun , Qin Bing , Li Wen , Zhang Jiaxuan , Li Wei , Wang Xiangyu , Zhang Maoying , Chen Gong 
Published: December 2023

Abstract

Transcription factor-mediated cell conversion has been reported in the central nervous system (CNS) of both rodents and non-human primates (NHPs). In particular, glia-to-neuron (GtN) conversion has been achieved in the brains and spinal cords of animal models of neurological disorders for neural regeneration and repair. However, whether GtN conversion can ultimately be used for human brain repair in patients is still unknown. To investigate the applicability of GtN conversion technology in the human brain, we established a long-term ex vivo culture system using human brain tissue that was surgically removed from epileptic patients to directly test GtN conversion. We demonstrate that both neural transcription factors NeuroD1 and Ascl1 can convert human glial cells into neurons. Importantly, both immunostaining and electrophysiological recordings revealed that the glia-converted neurons showed immature properties during the initial 1–2 weeks of conversion, and then acquired more mature neuronal properties after 3–4 weeks of conversion. These ex vivo conversion studies in human brain tissue pave the way toward future clinical trials using a transcription factor-based glia-to-neuron conversion approach to treat neurological disorders.
Association of Statin Use with 28-Day Mortality in the MIMIC-IV Database: A Cohort Study
Authors: kang chen , Jin Yiran , Yang Xiuling 
Published: December 2023

Abstract
Background
Statins, acknowledged for their cardiovascular risk reduction, exhibit pleiotropic effects, including anti-inflammatory, antithrombotic, and endothelial stabilizing actions. While intensive care unit (ICU) patients face heightened risks of cardiovascular disease, infections, and thrombotic complications, the impact of statin therapy on ICU mortality remains debated.
Aim
This retrospective cohort study, utilizing the Medical Information Mart for Intensive Care IV (MMIC-IV) database, aims to scrutinize the association between statin therapy and all-cause mortality in critically ill patients. Additionally, it endeavors to compare the impact of different statin types on mortality.
Method
Eligible ICU-admitted patients aged 18–90 were categorized based on statin usage during their ICU stay. The primary outcome was 28-day mortality, analyzed through multivariable Cox regression, providing adjusted hazard ratios (HR) with 95% confidence intervals (CI).
Results
Among 50,624 enrolled patients, 30.9% used statins. Statin treatment in ICU patients was correlated with reduced 28-day all-cause mortality in multivariate Cox analysis (Statins [HR] = 0.66, [95% CI, 0.61–0.70]; atorvastatin [HR] = 0.71, [95% CI, 0.66–0.78]; rosuvastatin [HR] = 0.57, [95% CI, 0.45–0.72]; simvastatin [HR] = 0.54, [95% CI, 0.48–0.62]; other statins [HR] = 0.68, [95% CI, 0.56–0.83]). Propensity score matching confirmed these findings (statins [HR] = 0.69, [95% CI, 0.63–0.75]).
Conclusion
Statin use may correlate with a decreased risk of 28-day mortality in ICU patients, with simvastatin showing a more pronounced effect. The robustness of these findings remain unaffected by subgroup analyses, sensitivity analyses, and propensity score matching, indicating potential clinical significance.
Food Insecurity in Urban American Indian and Alaska Native Populations During the COVID-19 Pandemic
Authors: Nelson Katie , Jackson Alexandra M. , Nguyen Cassandra J. , Noonan Carolyn , Muller Clemma , MacLehose Richard F , Manson Spero M. , Dillard Denise , Buchwald Dedra 
Published: December 2023

Abstract
Background
Food insecurity is an important social determinant of health that was exacerbated by the COVID-19 pandemic. Both food insecurity and COVID-19 infection disproportionately affect racial and ethnic minority groups, particularly American Indian and Alaska Native communities; however, there is little evidence as to whether food insecurity is associated with COVID-19 infection or COVID-19 preventive behaviors such as vaccination uptake. The purpose of this study was to evaluate associations between food insecurity, COVID-19 infection, and vaccination status among urban American Indian and Alaska Native adults seen at 5 clinics serving urban Native people.
Methods
In partnership with health organizations in Alaska, Colorado, Kansas, Minnesota, and New Mexico, the study team conducted a cross-sectional survey in 2021 to assess food security status and attitudes, barriers, and facilitators for COVID-19 testing and vaccination. Logistic regression was used to examine the association of food security status with sociodemographic factors and COVID-19 infection and vaccination status. Marginal standardization was applied to present results as prevalence differences.
Results
Among 730 American Indian and Alaska Native adults, the prevalence of food insecurity measured during the pandemic was 38%. For participants who reported persistent food security status before and during the pandemic (n=588), the prevalence of food insecurity was 25%. Prevalence of COVID-19 infection and vaccination did not vary by food security status after adjustment for confounders.
Conclusions
High rates of food insecurity among American Indian and Alaska Native communities likely increased during the COVID-19 pandemic. However, despite the high prevalence of food insecurity, community-led efforts to reduce COVID-19 infection and increase vaccination uptake across Indian Health Service and Tribal healthcare facilities may have mitigated the negative impacts of the pandemic for families experiencing food insecurity. These successful approaches serve as an important reference for future public health efforts that require innovative strategies to improve overall health in AIAN communities.
Faecalibaculum rodentium protects HSCs from IR-induced apoptosis via suppression of nuclear PKM2-enhanced p53 transcriptional activity
Authors: Zhu Hanyong , Sun Na , Chen Xin , Xiao Rui , Hui Guo , Ji Binbin , Yao Chen , Zhao Jie , Liu Zimeng , Gong Shengzhe , Qin Yongqin , Li Rongqing , Li Xueqin , Ikezoe Takayuki , Pan Yuchen , Yang Jing 
Published: December 2023

Abstract
It has been shown that under various conditions, such as aging, the gut flora contributes to modulating the status and functions of hematopoietic stem cells (HSCs). However, which species/strains of probiotics and the underlying mechanisms involved in controlling HSC function remain obscure, especially under irradiation conditions. In this study, we found that an antibiotic cocktail caused STAT3 localization in the mitochondria of epithelial cells along with a decrease in the abundance of F. rodentium. Feeding mice with F. rodentium and its metabolite butyrate prolonged the survival of irradiated mice. In a further study, we found that the administration of butyrate sustained nuclear PKM2 expression, attenuating p53-induced apoptosis. These results suggested that in response to irradiation, a reduced abundance of F. rodentium led to decreased butyrate content, thereby promoting PKM2 nuclear translocation, which subsequently enhanced p53 transcriptional activity.
Characteristics of regional heavy rainfall in the pre flood season in South China and prediction skill of NCEP S2S
Authors: Zheng Ting , Zheng Zhihai , Feng Guolin , Zhao Yuheng , Fan Peiyi 
Published: December 2023

Abstract
South China is one of the regions with the longest flood season and the highest frequency of floods in China. Understand the characteristics of heavy precipitation during the flood season and its potential mechanisms is crucial for predicting and preventing flood disasters in South China. In this study, we identified that the regional heavy precipitation during the first flood season in South China can be categorized into frontal heavy rainfall (FR) and warm-sector heavy rainfall (WR). The FR is mainly controlled by the southwest monsoon and the westerly jet in the northwest of the West Pacific Subtropical High (WPSH). The westerly wind brings enough water vapor to the front, creating an unstable environment that leads to heavy rainfall events. When precipitation occurs in warm regions, warm and humid air from the west side of the WPSH goes northward and westerly jet brings warm and humid air to the mainland to rise and condense, thus forming heavy rainfall. The extended period predictability of the National Centers for Environmental Prediction (NCEP) sub-seasonal to seasonal (S2S) forecast system for the two types of heavy rainfall decreases with the increase of the forecast lead time, and the prediction skill of NCEP S2S for FR is obviously better than that for WR. The poor prediction skill of NCEP S2S for WR is mainly due to the model inadequacies in predicting atmospheric circulation and water vapor transport anomalies corresponding to WR. When predicting WR, the model exhibits the easterly wind has controlled in the upper troposphere as early as ten days in advance. Meanwhile, the model struggles to predict the characteristics of the WPSH and the water vapor transport anomalies from the Bay of Bengal. This may be attributed to the absence of a distinct weather-scale boundary (front) for WR in the model, making it more difficult to forecast WR than FR.
A pilot study of the proarrhythmic effects of pimobendan injection in cats
Authors: Ishizaka Mio , Katagiri Keisuke , Ogawa Mizuki , Hsu Huai-hsun , Miyagawa Yuichi , Takemura Naoyuki 
Published: December 2023

Abstract

Pimobendan is not currently approved for cats, but its usefulness in feline hypertrophic cardiomyopathy has been suggested. An increase in arrhythmic events has also been reported after oral administration to healthy cats. Because intravenous administration has a more potent effect than oral administration, the incidence of arrhythmias after pimobendan injection may be increased, similar to oral pimobendan. Therefore, this study aimed to investigate the proarrhythmic effects of pimobendan injection in cats. Five cats underwent physical examination, echocardiography, blood pressure measurements and 24-h Holter electrocardiography before/after pimobendan administration as an intravenous bolus dose of 0.15 mg/kg twice daily for 3 days. After pimobendan administration, the 24-h total heart rate significantly increased. Echocardiography revealed a significant increase in mitral valve annulus systolic velocity (S') on the ventricular septal wall side, suggesting an increase in contractility. Only one cat had a lower tricuspid valve annulus S' on the free wall side of the right ventricle. This cat had paroxysmal ventricular tachycardia and an increase in the number of arrhythmic events. In other cats, the number of arrhythmias showed a decreasing trend. Therefore, when administering pimobendan to cats suspected of having myocardial dysfunction/heart disease, it may be necessary to closely monitor their electrocardiogram.
Python Toolbox for Android GNSS Raw Data to RINEX Conversion
Authors: Olcina Jorge Hernández , Julián Ana B. Anquela , Furones Ángel E. Martín 
Published: December 2023

Abstract
Global Navigation Satellite System (GNSS) data collected from Android devices has gained increasing importance in various applications, ranging from geospatial positioning to environmental monitoring. However, the lack of standardized tools for converting Android GNSS raw data into Receiver Independent Exchange (RINEX) format poses a significant challenge for researchers and practitioners. In response to this need, we present a comprehensive Python toolbox designed to streamline the conversion process and enhance the usability of Android GNSS data. The proposed toolbox leverages Python's versatility to provide a user-friendly interface for converting Android GNSS raw data into the widely adopted RINEX format. Key features include robust data parsing algorithms, support for multiple GNSS constellations, and compatibility with diverse Android device configurations. Furthermore, the toolbox's open-source nature encourages community collaboration and allows for continual improvement and adaptation to emerging GNSS technologies. We anticipate that this Python toolbox will serve as a valuable resource for researchers and practitioners working with Android GNSS data, facilitating standardized data interchange and promoting reproducibility in GNSS-based studies.
Using situational awareness and case-based seminars in a comprehensive nursing skills practice course to improve self-directed learning, academic self-efficacy, and professional identity in undergraduate nursing students
Authors: Sun Yuanhao , Li Xiangdong , Liu Haiyang , Li Yuqing , Gui Jiaofeng , Zhang Xiaoyun , Li Xiaoping , Sun Lu , Zhang Lin , Wang Congzhi , Li Jing , Liu Mingming , Zhang Dongmei , Gao Jingyi , Kang Xuefeng , Lei Yunxiao , Yuan Ting 
Published: December 2023

Abstract
Background: Nurses play an important role in healthcare development. The increasing demands for nurses mean that nursing schools at the undergraduate level have the responsibility to ensure patient safety and quality care through a well-designed curriculum. This research aimed to evaluate the effect of the teaching method combined with situational awareness and case-based seminars in a comprehensive nursing skills practice course on the level of self-directed learning, professional identity, academic self-efficacy, theoretical scores, practical scores, teaching satisfaction, and student competence among nursing students.
Methods: The research population comprised was of the grades of 2019 and 2020 at Wannan Medical College in Anhui Province, China (n =169, response rate 77.88%). The observation group from grade 2020 used the teaching method combined with situational awareness and case-based seminars, whereas the control group from grade 2019 used the traditional teaching mode. General information, self-directed learning, a professional identity, and academic self-efficacy were compared between the two groups. This research used means and standard deviations, chi-square, the Shapiro-Wilk test, and an independent sample t-test for statistical analyses.
Results: Compared with the control group, the total scores for self-directed learning, professional identity, and academic self-efficacy were higher in the observation group (78.80±7.89 vs 60.21±7.44, 63.39±7.87 vs 52.35±7.68, and 22.31±3.30 vs 21.28±2.31, respectively, with P ＜ 0.05 for all scores). More significant improvements were made in the observation group on the level of theoretical scores (81.39±3.32 vs 76.28±5.90) and practical scores (93.32±4.70 vs 90.67±5.09) (P ＜0.05). Meanwhile, teaching satisfaction, which includes teaching method (66/18 vs 32/53) and teacher-student interaction (72/12 vs 34/51), and student competence, which includes team cooperation (67/17 vs 39/46), critical thinking (60/24 vs 31/54), and communication skills (67/17 vs 38/47) after the intervention (P ＜0.05). There was no significant difference in social persuasion (P ＞0.05).
Conclusion: The teaching method combined with situational awareness and case-based seminars in a comprehensive nursing skills practice course has the potential to improve the level of self-directed learning, professional identity, and academic self-efficacy, and it increases theoretical scores, practical scores, teaching satisfaction, and student competence.