bluuebunny/crossref_metadata_2025_split huggingface dataset:
Limited preoperative knee extension in anterior cruciate ligament reconstruction using a hamstring tendon affects improvement of postoperative knee extensor strength
Authors: Sengoku Takuya , Nakase Junsuke , Mizuno Yushin , Kanayama Tomoyuki , Ishida Yoshihiro , Yanatori Yusuke , Arima Yu 
Published: October 2023

AbstractPurposeThis study aimed to determine the factors affecting knee extensor strength 6 months after anterior cruciate ligament (ACL) reconstruction using autograft hamstring tendon.Methods144 patients who could undergo regular follow‐up after ACL reconstruction were divided into 2 groups: those with greater than 90% (Group A: n = 95) and less than 85% (Group B: n = 49) isokinetic knee contraction at 60°/s 6 months post‐ACL reconstruction. Basic information, injury status, limited preoperative knee extension, and knee extensor strength at 3 and 6 months postoperatively were compared between the groups. Multivariate logistic analysis was performed and included variables that showed statistically significant differences between the groups in the univariate analysis. In addition, the cut‐off value for the limb symmetry index (LSI) at 3 months postoperatively needed to exceed an LSI of 90% at 6 months postoperatively was calculated using the receiver operating characteristics curve.ResultsAge, preoperative waiting period, limited preoperative knee extension, and knee extensor strength at 3 months postoperatively were significantly different between the two groups. The multivariate logistic analysis showed that all the variables affected the improvement in knee extensor strength at 6 months postoperatively. Limited preoperative knee extension was the most significant factor (odds ratio: 15.1, 95% confidence interval: 2.57–118.56, p < 0.01). The LSI cut‐off value at 3 months postoperatively was 72.0%.ConclusionKey factors in achieving the necessary knee extensor strength criteria for return to sports at 6 months post‐ACL reconstruction include addressing limited preoperative knee extension and achieving an LSI ≥ 72% in knee extensor strength at 3 months postoperatively.Level of evidenceLevel III.
MRI-detected intraosseous bone marrow edema recedes after effective therapy of periodontitis
Authors: Schwarting Julian , Probst Florian Andreas , Griesbauer Magdalena , Robl Teresa , Burian Egon , Wiestler Benedikt , Brunner Teresa , Malenova Yoana , Bumm Caspar , Folwaczny Matthias , Probst Monika 
Published: October 2023

Abstract
Objectives
T2 STIR MRI sequences can detect preclinical changes associated with periodontal inflammation, i.e. intraosseous edema in the tooth-supporting bone. In this study, we assessed whether MRI can be used for monitoring periodontal disease.

Material and methods
In a prospective cohort study, we examined 35 patients with periodontitis between 10/2018 and 04/2019 by using 3D isotropic T2-weighted short tau inversion recovery (STIR) and Fast Field Echo T1-weighted Black bone sequences. All patients received standardized clinical exams before and three months after non-surgical periodontal therapy. Bone marrow edema extent was quantified in the STIR sequence at 922 sites before and after treatment. Results were compared with standard clinical findings. Non-parametric statistical analysis was performed.

Results
Non-surgical periodontal treatment caused significant improvement in mean probing depth (p < 0.001) and frequency of bleeding on probing (p < 0.001). The mean depth of osseous edema per site was reduced from a median [IQR] of 2 [1, 3] mm at baseline to 1 [0, 3] mm, (p < 0.001). Periodontal treatment reduced the frequency of sites with edema from 35 to 24% (p < 0.01).

Conclusion
The decrease of periodontal bone marrow edema, as observed with T2 STIR MR imaging, is indicative of successful periodontal healing.

Clinical relevance statement
T2 STIR hyperintense bone marrow edema in the periodontal bone decreases after treatment and can therefore be used to evaluate treatment success. Furthermore, MRI reveals new options to depict hidden aspects of periodontitis.

Key Points
• T2 STIR hyperintense periodontal intraosseous edema was prospectively investigated in 35 patients with periodontitis before and after treatment and compared to clinical outcomes.
• The frequency of affected sites was reduced from 35 to 24% (p < 0.001), and mean edema depth was reduced from a median [IQR] of 2 [1, 3] mm at baseline to 1 [0, 3] mm 3 months after treatment. (p < 0.001).
• T2 STIR sequences can be used to monitor the posttreatment course of periodontitis.
Preoperative screening and prehabilitation strategies prior to ileocolic resection in patients with Crohn’s disease are not incorporated in routine care
Authors: Bak Michiel Thomas Jan , van Ruler Oddeke , Stassen Laurents , Ruiterkamp Marit , Arkenbosch Jeanine Hubertina Catharina , Dijkstra Gerard , Campmans-Kuijpers Maria Johanna Elisabeth , van Meeteren Nico Leonard Ulrich , Bongers Bart Chateau , Romberg-Camps Mariëlle , van der Marel Sander , Hoentjen Frank , van Dongen Koen Willem , West Rachel , van der Woude Janneke , de Vries Annemarie Charlotte ,   on behalf of the Dutch Initiative Crohn and Colitis (ICC) and Dutch Initiative on Crohn and Colitis – Surgery (ICC-S)
Published: October 2023

Abstract
Purpose
Recently, recommendations on perioperative care have been published to optimize postoperative outcomes in preoperative patients with inflammatory bowel disease. This study evaluated the current use of preoperative screening and prehabilitation strategies (PS) prior to elective ileocolic resection (ICR) in patients with Crohn’s disease (CD).

Methods
Patients with CD who underwent an elective ICR were identified from a Dutch prospective cohort study. Primary endpoint was to evaluate to what extent IBD-relevant PS were applied in patients with CD prior to ICR according to the current recommendations.

Results
In total, 109 CD patients were included. Screening of nutritional status was performed in 56% of the patients and revealed malnutrition in 46% of these patients. Of the malnourished patients, 46% was referred to a dietitian. Active smoking and alcohol consumption were reported in 20% and 28%; none of these patients were referred for a cessation program. A preoperative anemia was diagnosed in 61%, and ferritin levels were assessed in 26% of these patients. Iron therapy was started in 25% of the patients with an iron deficiency anemia. Exposure to corticosteroids at time of ICR was reported in 29% and weaned off in 3%. Consultation of a dietitian, psychologist, and physiotherapist was reported in 36%, 7%, and 3%. Physical fitness was assessed in none of the patients.

Conclusion
PS are not routinely applied and not individually tailored in the preoperative setting prior to elective ICR in patients with CD. Prior to implementation, future research on the costs and effectiveness of PS on postoperative outcomes and quality of life is necessary.
Survival outcome of local versus radical resection for jejunoileal gastrointestinal stromal tumors: a propensity score-matched population-based analysis
Authors: Yan Shangcheng , Peng Wei , Cheng Ming , Zhang Jingyu , Liu Tianhua , Sheng Mengchao , Ren Rui , Chen Qiang , Gong Wei , Wu Yongyou 
Published: October 2023

Abstract
Purpose
Survival after local resection (LR) versus radical resection (RR) has been revealed comparable for patients with rectal and duodenal gastrointestinal stromal tumors (GISTs), but is unknown for jejunoileal (JI) GISTs. This study aimed to compare the long-term survival between patients with JI GISTs who underwent LR and RR, and to find out the prognostic factors for JI GISTs.

Methods
Patients diagnosed with JI GISTs in 1975–2019 were identified from Surveillance, Epidemiology, and End Results (SEER) database and grouped according to surgical modality. Propensity score matching (PSM) was performed to balance the LR and RR groups. Overall survival (OS) and disease-specific survival (DSS) were compared in the full and matched cohorts using Kaplan–Meier (KM) analysis. Subgroup sensitivity analyses were also performed. Risk factors associated with DSS were analyzed in multivariate Cox analysis following model selection.

Results
1107 patients diagnosed with JI GISTs were included in the study cohort. After PSM, OS and DSS were comparable in LR and RR groups. Consistently, the two groups had similar DSS in all subgroup analyses. Moreover, multivariate Cox analysis identified lymphadenectomy, older age, larger tumor size, distant metastasis, high and unknown mitotic rate, but not LR, as independent prognostic risk factors for JI GISTs.

Conclusions
We conducted the first population-based comparison between the effect of different surgical modes on survival for patients with JI GISTs. LR can be carried out safely without compromising oncological outcome, and should be considered as a treatment option in selected patients with JI GISTs.
Human cases of lymphocytic choriomeningitis virus (LCMV) infections in Hungary
Authors: Pankovics Péter , Nagy Arnold , Nyul Zoltán , Juhász Annamária , Takáts Károly , Boros Ákos , Reuter Gábor 
Published: October 2023

AbstractLymphocytic choriomeningitis (LCM) is a “neglected” rodent-borne viral zoonotic disease caused by lymphocytic choriomeningitis virus (LCMV) (family Arenaviridae). The aim of this retrospective clinical and laboratory study was to detect LCMV RNA, using RT-PCR, in cerebrospinal fluid samples collected from patients with central nervous system (CNS) infections of unknown aetiology from over a 12-year period in Hungary. Between 2009 and 2020, a total of 74 cerebrospinal fluid samples were tested using an in-house LCMV-specific RT-PCR-based method at the Department of Medical Microbiology and Immunology, University of Pécs. The mean age of the 74 patients included in our study was 24 years (min. 5, max. 74), with a predominance of men (44 [59.5%]; women, 30 [40.5%]). Two (2.7%) cerebrospinal fluid samples were found to be positive for LCMV RNA by RT-PCR and sequencing. The first LCMV case was a 5-year-old preschool boy who had a hamster bite on his left-hand finger, and the second LCMV case was a 74-year-old man who was living in a village and had incipient dementia and a previous permanent functional CNS impairment. The two detected LCMV strains (MW558451 and OM648933) from the year 2020 belonged to two different genetic lineages (I and II). These two cases of CNS inflammation of unknown origin represent the first published human LCMV infections confirmed by molecular methods in Hungary.
Collision probability reduction method for tracking control in automatic docking/berthing using reinforcement learning
Authors: Wakita Kouki , Akimoto Youhei , Rachman Dimas M. , Miyauchi Yoshiki , Maki Atsuo 
Published: October 2023

AbstractAutomation of berthing maneuvers in shipping is a pressing issue as the berthing maneuver is one of the most stressful tasks seafarers undertake. Berthing control problems are often tackled by tracking a predefined trajectory or path. Maintaining a tracking error of zero under an uncertain environment is impossible; the tracking controller is nonetheless required to bring vessels close to desired berths. The tracking controller must prioritize the avoidance of tracking errors that may cause collisions with obstacles. This paper proposes a training method based on reinforcement learning for a trajectory tracking controller that reduces the probability of collisions with static obstacles. Via numerical simulations, we show that the proposed method reduces the probability of collisions during berthing maneuvers. Furthermore, this paper shows the tracking performance in a model experiment.
Neutralizing anti-IFN-γ IgG was increased in patients with systemic lupus erythematosus and associated with susceptibility to infection
Authors: Chen Longfang , Chi Huihui , Teng Jialin , Meng Jianfen , Zhang Hao , Su Yutong , Liu Honglei , Ye Junna , Shi Hui , Hu Qiongyi , Zhou ZhuoChao , Yang Chengde , Sun Yue , Cheng Xiaobing 
Published: October 2023

Abstract
Objectives
Systemic lupus erythematosus (SLE) is a complicated autoimmune disease, in which infection is a leading cause of death. Some SLE patients clinically presented with recurrent and refractory infections, which manifested as adult-onset immunodeficiency syndrome due to the production of anti-interferon-γ (anti-IFN-γ) autoantibodies. This study aimed to investigate the role of anti-IFN-γ autoantibodies concerning severe infections in SLE patients.

Methods
We detected serum levels of anti-IFN-γ IgG/IgM isotypes in SLE patients with severe infections (n = 55), SLE patients without severe infections (n = 120), rheumatoid arthritis (n = 24), ankylosing spondylitis (n = 24), and healthy controls (n = 60). The relationship between anti-IFN-γ autoantibodies and clinical characteristics and laboratory parameters were analyzed. We further evaluated the neutralizing ability of anti-IFN-γ IgG.

Results
The level of anti-IFN-γ IgG was significantly elevated in SLE patients with severe infections compared with the other groups (all p < 0.01), and the positive rates of anti-IFN-γ IgG in SLE patients with and without severe infections were 29.1% and 10.8%, respectively. Further analysis indicated that the levels of anti-IFN-γ IgG were positively associated with the SLEDAI score (r = 0.6420, p < 0.001), and it could predict the susceptibility to severe infections in SLE patients. Moreover, the inhibition and function assay showed that purified IgG from anti-IFN-γ IgG-positive SLE patients could neutralize IFN-γ, and further impair IFN-γ-induced STAT1 phosphorylation.

Conclusions
The neutralizing anti-IFN-γ IgG might increase the susceptibility to infection in SLE patients, which has important implications for the treatment.

Key Points• The role of anti-IFN-γ autoantibodies concerning severe infections in SLE patients remains unknown.• The results of this study reveals that anti-IFN-γ IgG levels were significantly elevated in SLE patients with severe infections.• This study suggests that neutralizing anti-IFN-γ IgG might increase the susceptibility to infection in SLE patients.
Country-wide expansion of a VIM-1 carbapenemase-producing Klebsiella oxytoca ST145 lineage in Poland, 2009–2019
Authors: Biedrzycka M. , Urbanowicz P. , Żabicka D. , Hryniewicz W. , Gniadkowski M. , Izdebski R. 
Published: October 2023

Abstract
Purpose
To elucidate the role of the Klebsiella oxytoca species complex (KoSC) in epidemiology of VIM-type MBL-producing Enterobacterales in Poland.

Methods
The study comprised all 106 VIM-positive KoSC isolates collected by the Polish National Reference Centre for Susceptibility Testing during 2009–2019 from 60 institutions in 35 towns. All isolates were sequenced by Illumina MiSeq, followed by MinION sequencing of selected organisms. Genomes were subjected to bioinformatic analysis, addressing taxonomy, clonality, phylogeny and structural characterisation of key resistance determinants within their chromosomal and plasmidic loci.

Results
Among five species identified, K. oxytoca was predominant (n = 92), followed by Klebsiella michiganensis (n = 11). MLST distinguished 18 STs, with the most prevalent Klebsiella oxytoca ST145 (n = 83). The clone segregated a lineage with the In237-like integron [blaVIM-1–aacA4 genes; n = 78], recorded in 28 cities almost all over the country. The integron was located in a ~ 49–50 kb chromosomal mosaic region with multiple other resistance genes, linked to a ~ 51 kb phage-like element. The organism might have originated from Greece, and its evolution in Poland included several events of chromosomal ~ 54–258 kb deletions, comprising the natural β-lactamase blaOXY gene. A group of other isolates of various species and clones (n = 12) carried the integron In916 on self-transmissible IncA-type plasmids, effectively spreading in Italy, France and Poland.

Conclusion
KoSC has been one of the major VIM producers in Poland, owing largely to clonal expansion of the specific K. oxytoca–In237-like lineage. Its apparently enhanced epidemic potential may create a danger on international scale.
High risk of intestinal colonization with ESBL-producing Escherichia coli among soldiers of military contingents in specific geographic regions
Authors: Literacka E. , Konior M. , Izdebski R. , Żabicka D. , Herda M. , Gniadkowski M. , Korzeniewski K. 
Published: October 2023

AbstractOne-hundred Polish soldiers of a contingent in Afghanistan in 2019 were screened for Enterobacterales resistant to newer-generation β-lactams at their departure and return. Seventeen percent were colonized in the gut at the departure, whereas 70% acquired carriage in Afghanistan. The commonest organisms were extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-Ec; 96.6%). All isolates were sequenced and were clonally diverse overall, even within the same sequence type, indicating that independent acquisitions mainly. ESBL-Ec were often multi-drug-resistant. Soldiers stationing in certain regions are at high risk of acquiring resistant bacteria that may cause endogenous infection, be transmitted to vulnerable individuals, and spread resistance genes.
Productivity analysis: roots, foundations, trends and perspectives
Authors: Zelenyuk Valentin 
Published: October 2023

AbstractThe goal of this article is to give a brief overview of productivity analysis, starting with general concepts, its importance and a brief historical excursion and then focusing on various productivity indexes. We also start from very simple productivity indexes to more sophisticated, such as Malmquist Productivity Indexes, which are among the most popular in academic literature these days. A special attention is on the contributions to this literature from Rolf Färe and Shawna Grosskopf (and their many co-authors), and some of the related works they have inspired.A brief discussion of likely perspectives for the area is also provided.
Pandemieforschung: von Autoantikörpern und Impfstoff-Innovationen
Authors: de la Rosa Kathrin 
Published: October 2023

AbstractThe study of human antibody repertoires and the underlying mechanisms of diversification are key to understand immune dysregulation, as well as to improve vaccine designs. This article outlines, how the interaction of antibodies and vaccines with body-own structures affects disease pathogenesis and vaccine performance. It is discussed why autoantibodies in COVID-19 should be discriminated in specific-pathogenic versus promiscuous-harmless, and how a strategy called BIBAX improves vaccines by design.
Untersuchung der Liganden-Bindung am MHC-Klasse I-Protein
Authors: Borchert Britta , Springer Sebastian 
Published: October 2023

AbstractAnalysis of receptor-ligand interactions is fundamental for the understanding of many biological processes. Our main object of investigation are MHC class I proteins, cell surface receptors and key players of the adaptive immune system. Their ligands are antigenic peptides presented to immune cells for revelation of infection. Ligand binding is inevitable for structural integrity and stability of the MHC class I receptor. In this article, we describe expedient methods for MHC class I analysis: nano-DSF, microscale thermophoresis, and fluorescence anisotropy.
IMS meets HKA: ein neues Tool zur Überwachung von Mikroalgenkulturen
Authors: Mayer Thomas , Steingroewer Juliane , Petrich Ralf , Borsdorf Helko 
Published: October 2023

AbstractA new method for real-time monitoring of algae cultures in bioreactors has been developed. It is based on ion mobility spectrometry and enables the detection of volatile organic compounds released by the algae. Spectral analysis and principal component analysis can be used to identify growth phases and to detect experimental influences. This continuous monitoring enables early detection of disturbances in the biotechnological process.
Nanokörper – klein, aber vielfältig einsetzbar
Authors: Buser Dominik P. 
Published: October 2023

AbstractNanobodies represent the variable domains of heavy-chain-only antibodies and possess the potential to surmount several shortcomings of conventional antibodies. Due to their exceptionally small size, structure, solubility, robust stability, high antigen-binding affinity, and ease of recombinant manipulation and expression, they are not only well suited for biomedical applications, but also provide sophisticated tools for basic research in molecular biology. Here, we discuss applications based on engineered and derivatized nanobodies as tools to systematically study biological processes in the cell.
Trispezifische Antikörper in der Immuntherapie: Drei sind keiner zu viel
Authors: Stadlbauer Katharina , Natale Veronica , Rüker Florian , Wozniak-Knopp Gordana 
Published: October 2023

AbstractBispecific antibodies revolutionized immunotherapy by improving the specificity of target recognition and extending the spectrum of treatments by enabling efficient target and effector cell interaction. Engineering strategies have led to novel designs of tri- and multispecific antibodies, which combine even more beneficial properties and promise superior efficacy and safety. Several of them efficiently harvest the patient’s immune response by addressing effector cells of the innate immune system.
Aktivierung natürlicher Killerzellen zur Behandlung von Tumorerkrankungen
Authors: Harwardt Julia , Schoenfeld Katrin , Kolmar Harald 
Published: October 2023

AbstractImmunotherapy represents a promising tool significantly improving cancer outcomes. The concept of tumor recognition and elimination by harnessing the patients’ immune system has recently been applied to cells of the innate immunity. Particularly, antibody-based NK cell engagers have been explored which specifically target tumor-associated antigens and simultaneously receptors on natural killer cells boosting the immune effector cells’ cytolytic effect and further inducing a multicellular immune response.
Der Crumbs-Komplex – von Epithelpolarität zu retinaler Degeneration
Authors: Knust Elisabeth 
Published: October 2023

AbstractThe evolutionarily conserved Crumbs protein complex, initially identified in Drosophila, is a crucial regulator of epithelial cell polarity and shape in both invertebrates and vertebrates. Mutations are associated with various diseases, which are based on epithelial dysfunction, such as tumorigenesis, and result in retinal degeneration in humans, mice and flies. The plethora of its interaction partner defines the Crumbs complex as an apical hub to coordinate polarity, adhesion and signalling.
Early detection of dyslexia based on EEG with novel predictor extraction and selection
Authors: Parmar Shankar , Paunwala Chirag 
Published: October 2023

AbstractDyslexia is a learning disorder caused by difficulties in the brain’s processing of letters and words. This study used EEG recordings to detect dyslexia at a young age. EEG recordings of 53 individuals, including 29 dyslexic and 24 normal individuals, were collected while they were engaged in two distinct mental activities known as the N-Back task and the Oddball task. Predictors were extracted using several methods and reduced using Principal Component Analysis (PCA). A relief-based strategy was applied to select predictors, and Support Vector Machine (SVM) classifier was used to achieve an average accuracy of 79.3% for dyslexia detection, which is better than the performance of its predecessors. The results indicate that EEG recordings and machine learning methods could be useful for identifying dyslexia in children.