bluuebunny/crossref_metadata_2025_split huggingface dataset:
CTXP, The Major Cobra Toxin Peptide from Naja Naja Oxiana Venom; A Promising Target for Antivenom Agent Development
Authors: Chafi Mohammad Hosseininejad , Eslamnezhad-Namin Mohsen , Dastjerdeh Mansoureh Shahbazi , Zareinejad Mohammad Reza , Oghalaie Akbar , Bagheri Kamran Pooshang , Kazemi-Lomedasht Fatemeh , Karimi Gholamreza , Razzaghi-Abyaneh Mehdi , Seyedjavadi Sima Sadat , Behdani Mahdi 
Published: January 2024

Background and Objective:
Snakebite envenoming is a serious public health issue causing
more than 135,000 annual deaths worldwide. Naja naja oxiana is one of the most clinically important
venomous snakes in Iran and Central Asia. Conventional animal-derived polyclonal antibodies
are the major treatment of snakebite envenoming. Characterization of venom components
helps to pinpoint the toxic protein responsible for clinical manifestations in victims, which aids us
in developing efficient antivenoms with minimal side effects. Therefore, the present study aimed
to identify the major lethal protein of Naja naja oxiana by top-down proteomics.

Methods:
Venom proteomic profiling was performed using gel filtration (GF), reversed-phase (RP)
chromatography, and intact mass spectrometry. The toxicity of GF-, and RP-eluted fractions
was analyzed in BALB/c mice. The rabbit polyclonal antisera were produced against crude venom,
GF fraction V (FV), and RP peak 1 (CTXP) and applied in neutralization assays.

Results:
Toxicity studies in BALB/c identified FV as the major toxic fraction of venom. Subsequently,
RP separation of FV resulted in eight peaks, of which peak 1, referred to as “CTXP” (cobra
toxin peptide), was identified as the major lethal protein. In vivo neutralization assays using
rabbit antisera showed that polyclonal antibodies raised against FV and CTXP are capable of neutralizing
at least 2-LD50s of crude venom, FV, and CTXP in all tested mice.

Conclusion:
Surprisingly, the Anti-CTXP antibody could neutralize 8-LD50 of the CTXP peptide.
These results identified CTXP (a 7 kDa peptide) as a potential target for the development of
novel efficient antivenom agents.
Roles of Accelerated Molecular Dynamics Simulations in Predictions of
Binding Kinetic Parameters
Authors: Chen Jianzhong , Wang Wei , Sun Haibo , He Weikai 
Published: January 2024

Abstract:
Rational predictions on binding kinetics parameters of drugs to targets play significant
roles in future drug designs. Full conformational samplings of targets are requisite for accurate predictions
of binding kinetic parameters. In this review, we mainly focus on the applications of enhanced
sampling technologies in calculations of binding kinetics parameters and residence time of
drugs. The methods involved in molecular dynamics simulations are applied to not only probe conformational
changes of targets but also reveal calculations of residence time that is significant for
drug efficiency. For this review, special attention are paid to accelerated molecular dynamics (aMD)
and Gaussian aMD (GaMD) simulations that have been adopted to predict the association or disassociation
rate constant. We also expect that this review can provide useful information for future
drug design.
Calcium Modulating Effect of Polycyclic Cages: A Suitable Therapeutic
Approach Against Excitotoxic-induced Neurodegeneration
Authors: Egunlusi Ayodeji O. , Malan Sarel F. , Palchykov Vitalii A. , Joubert Jacques 
Published: January 2024

Abstract:
Neurodegenerative disorders pose a significant challenge to global healthcare systems
due to their progressive nature and the resulting loss of neuronal cells and functions. Excitotoxicity,
characterized by calcium overload, plays a critical role in the pathophysiology of these disorders. In
this review article, we explore the involvement of calcium dysregulation in neurodegeneration and
neurodegenerative disorders. A promising therapeutic strategy to counter calcium dysregulation involves
the use of calcium modulators, particularly polycyclic cage compounds. These compounds,
structurally related to amantadine and memantine, exhibit neuroprotective properties by attenuating
calcium influx into neuronal cells. Notably, the pentacycloundecylamine NGP1-01, a cage-like
structure, has shown efficacy in inhibiting both N-methyl-D-aspartate (NMDA) receptors and voltage-
gated calcium channels (VGCCs), making it a potential candidate for neuroprotection against
excitotoxic-induced neurodegenerative disorders. The structure-activity relationship of polycyclic
cage compounds is discussed in detail, highlighting their calcium-inhibitory activities. Various
closed, open, and rearranged cage compounds have demonstrated inhibitory effects on calcium influx
through NMDA receptors and VGCCs. Additionally, these compounds have exhibited neuroprotective
properties, including free radical scavenging, attenuation of neurotoxicities, and reduction
of neuroinflammation. Although the calcium modulatory activities of polycyclic cage compounds
have been extensively studied, apart from amantadine and memantine, none have undergone clinical
trials. Further in vitro and in vivo studies and subsequent clinical trials are required to establish the
efficacy and safety of these compounds. The development of polycyclic cages as potential multifunctional
agents for treating complex neurodegenerative diseases holds great promise.
Traditional Herbal Medications Utilized in the Indian Medical System for
the Management of Diabetes: An Updated Review and Clinical Implications
Authors: Sachan Kapil , Verma Suryakant , Arora Kunal , Bharti Ujjwal , Singh Pranjal Kumar , Singh Smita 
Published: January 2024

Abstract:
Phytomedicine, also called botanical medicine, is the practice of using plants to treat
disease. Diabetes, for example, has been treated and prevented with herbal medication for a lot
longer than Western medicine. Worldwide, diabetes has become a major health concern. The management
of diabetes and hyperglycemia, two of the most common public health threats, is far from
ideal. When hyperglycemia persists or is not under control, diabetes-related complications, like
blindness, lower limb amputations, renal disease, and cardiovascular disease, play a significant
role in the morbidity and mortality of the disease. Although chemicals and biochemical agents can
assist in managing diabetes, there is currently no complete cure for the disease. Herbal remedies
are one of many methods that can be used to treat and prevent diabetes and its subsequent problems.
Numerous traditional treatments have been discovered for diabetes as a result of extensive
research efforts. However, there are many factors to consider when deciding which herbs to use,
such as the patient's financial status, the presence or absence of co-morbidities, and the accessibility,
cost-effectiveness, and safety profile of the herbs. This article focuses on the use of herbal and
natural remedies in the treatment and prevention of diabetes, the mechanisms by which these remedies
lower blood glucose levels, and the specific herbal items now utilized in the management of
diabetes.
Unravelling the Crosstalk between Estrogen Deficiency and Gut-biota
Dysbiosis in the Development of Diabetes Mellitus
Authors: Rishabh  , Bansal Seema , Goel Akriti , Gupta Sumeet , Malik Deepti , Bansal Nitin 
Published: January 2024

Abstract:
Estrogens are classically considered essential hormonal signals, but they exert profound
effects in a number of physiological and pathological states, including glucose homeostasis
and insulin resistance. Estrogen deficiency after menopause in most women leads to increased
androgenicity and changes in body composition, and it is recommended to manipulate the β-cell
function of the pancreas, insulin-induced glucose transport, and hepatic glucose output, hence, the
increasing incidence of type 2 diabetes mellitus. Recently, studies have reported that gut biota
alteration due to estrogen deficiency contributes to altered energy metabolism and, hence, accentuates
the pathology of diabetes mellitus. Emerging research suggests estrogen deficiency via
genetic disposition or failure of ovaries to function in old age modulates the insulin resistance and
glucose secretion workload on pancreatic beta cells by decreasing the levels of good bacteria such
as Akkermansia muciniphila, Bifidobacterium spp., Lactobacillus spp., Faecalibacterium
prausnitzii, Roseburia spp., and Prevotella spp., and increasing the levels of bad bacteria’s such as
Bacteroides spp., Clostridium difficile, Escherichia coli, and Enterococcus spp. Alteration in these
bacteria's concentrations in the gut further leads to the development of impaired glucose uptake
by the muscles, increased gluconeogenesis in the liver, and increased lipolysis and inflammation
in the adipose tissues. Thus, the present review paper aims to clarify the intricate interactions
between estrogen deficiency, gut microbiota regulation, and the development of diabetes mellitus.
Novel Strategies to Improve the Cardioprotective Effects of Cardioplegia
Authors: Osorio-Llanes Estefanie , Castellar-López Jairo , Rosales Wendy , Montoya Yuliet , Bustamante John , Zalaquett Ricardo , Bravo-Sagua Roberto , Riquelme Jaime A. , Sánchez Gina , Chiong Mario , Lavandero Sergio , Mendoza-Torres Evelyn 
Published: January 2024

Abstract:
The use of cardioprotective strategies as adjuvants of cardioplegic solutions has become
an ideal alternative for the improvement of post-surgery heart recovery. The choice of the
optimal cardioplegia, as well as its distribution mechanism, remains controversial in the field of
cardiovascular surgery. There is still a need to search for new and better cardioprotective methods
during cardioplegic procedures. New techniques for the management of cardiovascular
complications during cardioplegia have evolved with new alternatives and additives, and each
new strategy provides a tool to neutralize the damage after ischemia/reperfusion events. Researchers
and clinicians have committed themselves to studying the effect of new strategies and
adjuvant components with the potential to improve the cardioprotective effect of cardioplegic
solutions in preventing myocardial ischemia/reperfusion-induced injury during cardiac surgery.
The aim of this review is to explore the different types of cardioplegia, their protection mechanisms,
and which strategies have been proposed to enhance the function of these solutions in
hearts exposed to cardiovascular pathologies that require surgical alternatives for their corrective
progression.
Nuchal Translucency and Congenital Heart Defects
Authors: Sofia-Gonçalves A. , Guedes-Martins L. 
Published: January 2024

Abstract:
Nuchal translucency comprises a temporary accumulation of fluid in the subcutaneous
tissue on the back of a fetus’s neck, which accompanies the crown-rump length and is observed
through an ultrasound performed between 11 and 13 weeks + 6 days gestation. Nuchal
translucency is considered to be above normal when values are higher than the 95th/99th percentile
or equal to or higher than 2.5/3.5 mm. The first connection between increased nuchal
translucency and the presence of congenital heart defects is described in the study of Hyett et
al., who observed that they are directly proportional. Since that time, several studies have been
conducted to understand if nuchal translucency measurements can be used for congenital heart
defect screening in euploid fetuses. However, there is great variability in the estimated nuchal
translucency cutoff values for congenital heart defect detection. The purpose of this review was
to understand how increased nuchal translucency values and congenital heart defects are related
and to identify which of these defects are more frequently associated with an increase in these
values.
Cardiovascular Symptoms, Dysautonomia, and Quality of Life in Adult
and Pediatric Patients with Hypermobile Ehlers-Danlos Syndrome:
A Brief Review
Authors: Hertel Amanda , Black William R. , Malloy Walton Lindsey , Martin Julie R. , Jones Jordan T. 
Published: January 2024

Background::
Hypermobile Ehlers-Danlos Syndrome (hEDS) is a connective tissue
disorder characterized by joint hypermobility and other systemic manifestations, such as cardiovascular
symptoms, musculoskeletal pain, and joint instability. Cardiovascular symptoms, such as
lightheadedness and palpitations, and types of dysautonomia, including postural orthostatic tachycardia
syndrome (POTS), are frequently reported in adults with hEDS and have been shown to
negatively impact quality of life (QoL).

Objective::
This brief review will be an overview of co-occurring symptoms in POTS and hEDS
to inform potential cardiovascular screening procedures.

Results::
While many patients with hEDS report cardiovascular symptoms, few have structural
abnormalities, suggesting that dysautonomia is likely responsible for these symptoms. One validated
screening measure for dysautonomia symptom burden is the Composite Autonomic Symptom
Scale (COMPASS-31). Studies have found that adults with POTS, hEDS, and both POTS
and hEDS have higher COMPASS-31 scores than the general population, suggesting a high
symptom burden due to dysautonomia, which leads to impaired QoL.

Conclusion::
While studies have examined cardiovascular symptoms and the impact of dysautonomia
in adults with and without hEDS, there is scant literature on dysautonomia in pediatric
patients with hEDS. Therefore, more studies on cardiovascular symptoms and dysautonomia, as
they relate to the quality of life in pediatric patients with hEDS, are needed. This brief review
summarizes the current literature on dysautonomia and cardiovascular symptoms in pediatric and
adult populations with hEDS.
Green Synthesis Derived Novel Fe2O3/ZnO Nanocomposite for Efficient
Photocatalytic Degradation of Methyl Orange Dye
Authors: Jadon Nimisha , Kour Bhupinder , Bhat Bilal Ahmad , Sharma Harendra K. 
Published: January 2024

Introduction:
An eco-friendly method was reported for the synthesis of ferric oxide nanoparticles
(Fe2O3), zinc oxide nanoparticles (ZnO) and Fe2O3/ZnO nanocomposite using Mangifera
indica plant leaf extract as a natural reducing agent.

Methods:
The synthesized nanomaterials were successfully characterized by X-ray diffraction, UVvisible
spectrophotometer, Photoluminescence spectroscopy and Transmission electron microscopy.
The obtained XRD spectrums revealed the crystalline nature of synthesized materials and the average
diameters of Fe2O3 nanoparticles, ZnO nanoparticles and Fe2O3/ZnO nanocomposite came out to
be 11.33 nm, 14.31 nm and 9.80 nm, respectively. The UV-visible absorbance spectra and photoluminescence
spectrums confirmed that the Fe2O3/ZnO nanocomposite was visible light active and had
excitation peaks in the visible range.

Results:
The TEM analysis confirmed the composite and semiconductor nature of the synthesized
Fe2O3/ZnO nanocomposite. Furthermore, the photocatalytic activity of Fe2O3/ZnO nanocomposite
reaches about 91.07% degradation of methyl orange dye within a time period of 150 min at an optimized
catalyst dose. Adsorption isotherm and kinetic study were also applied to validate the study.

Conclusion::
It was found that there was monolayer adsorption of methyl orange dye molecules on
the surface of the synthesized catalyst under optimized experimental conditions and also, the adsorption
process follows the pseudo-second-order kinetic model.
Antimetastatic Drug, Pharmacologic Challenge and Opportunity
Authors: Lu Da-Yong , Lu Ting-Ren 
Published: January 2024

:
Cancer is a complex and high-mortality disease in the clinic. Cancer metastasis leads to
most cancer deaths. The therapeutics for cancer metastasis are greatly unsatisfactory now. Despite
different types of antimetastatic agents and drugs have been reported, 90% of patients die in 5 years
after metastatic nodules at secondary sites have been found.

:
Many pharmacologic challenges and opportunities for current metastasis therapies are presented. To
overcome the dilemma and shortcomings of antimetastatic treatment, medical, chemical, pharmaceutical,
methodological and technical issues are integrated and highlighted. To introduce up-to-date
knowledge and insights into drug targeting and pharmaceutical features and clinical paradigms, relevant
drug design insights are discussed—including different pathological modes, diagnosis advances,
metastatic cascade, tumor plasticity, variety of animal models, therapeutic biomarkers, computational
tools and cancer genomics. Integrated knowledge, systems and therapeutics are focused.

:
In summary, medicinal comparison, pharmaceutical innovation and clinical strategies should be increasingly
investigated.
Endothelial Cell Aging and Autophagy Dysregulation
Authors: Marzoog Basheer Abdullah 
Published: January 2024

:
Entropy is a natural process that affects all living cells, including senescence, an irreversible
physiological process that impairs cell homeostasis. Age is a significant factor in disease
development, and the pathogenesis of endothelial cell aging is multifactorial. Autophagy dysfunction
accelerates endothelial cell aging and cell death, while autophagy preserves endothelial
cell youthfulness through intracellular homeostasis and gene expression regulation. Sirt,
mTORC1, and AMPK are youthfulness genes that induce autophagy by inhibiting mTOR and
upregulating FIP200/Atg13/ULK1. Aged endothelial cells have decreased levels of Lamin B1,
γH2AX, Ki67, BrdU, PCNA, and SA β-Gal. Maintaining healthy young endothelial cells can
prevent most cardiovascular diseases. Autophagy targeting is a potential future therapeutic strategy
to modify endothelial cell age and potentially slow or reverse the aging process. This article
provides state-of-the-art research on the role of autophagy in endothelial cell aging. Hypothesizing
that autophagy dysregulation is associated with early endothelial cell dysfunction and further
clinical sequelae, including atherosclerosis formation, leading to various cardiovascular diseases.
Comunicação no Facebook dos governos federal brasileiro e estadual paulista
Authors: Medrado Andrea Meyer , Cabral Filho Adilson Vaz 
Published: January 2024

São abordados temas presentes nas páginas do Governo Federal do Brasil e do Estado de São Paulo no Facebook durante a pandemia da Covid-19, a partir de observações netnográficas e análise de conteúdo. São enfatizadas questões econômicas e de saúde, relacionadas com a pandemia nestes dois níveis - Federal e Estadual - de governo. O artigo incorpora noções de "sociedade incivil" e "necropolítica", analisando como estas se manifestam em um contexto de reformas neoliberais aceleradas. As conclusões demonstram que o Governo Federal se concentrou em medidas econômicas enquanto havia pouca cobertura de questões de saúde, tais como prevenção, orientação e vacinas. Em contraste, o Estado de São Paulo tinha uma predominância de postos sobre vacinação. Para ambas as campanhas, estas foram percebidas como estratégias de campanha permanente em vez de atender às necessidades dos cidadãos.
Investigating the Integration and the Long-Term Use of Smart Speakers in Older Adults’ Daily Practices: Qualitative Study
Authors: Chang Fangyuan , Sheng Lin , Gu Zhenyu 
Published: January 2024

Background
As smart speakers become more popular, there have been an increasing number of studies on how they may benefit older adults or how older adults perceive them. Despite the increasing ownership rates of smart speakers among older adults, studies that examine their integration and the long-term use in older adults’ daily practices are scarce.

Objective
This study aims to uncover the integration of smart speakers into the daily practices of older adults over the long term, contributing to an in-depth understanding of maintained technology use among this demographic.

Methods
To achieve these objectives, the study interviewed 20 older adults who had been using smart speakers for over 6 months. These semistructured interviews enabled participants to share their insights and experiences regarding the maintained use of smart speakers in the long term.

Results
We identified 4 dimensions of the long-term use of smart speakers among older adults, including functional integration, spatial integration, cognitive integration, and semantic integration. For the functional integration of smart speakers, the study reported different types of use, including entertainment, information collection, medication reminders, companionship, environment modification, and emergency calls. For the spatial integration of smart speakers, the study showed older adults’ agency in defining, changing, and reshaping daily practices through the spatial organization of smart speakers. For the cognitive integration of smart speakers, the findings showed the cognitive processes involved in adapting to and incorporating smart speakers into daily habits and routines. For the semantic integration of smart speakers, the findings revealed that older adults’ enjoyable user experience and strong bonds with the device contributed to their acceptance of occasional functional errors. Finally, the study proposed several suggestions for designers and developers to better design smart speakers that promote maintainable use behaviors among older adults.

Conclusions
On the basis of the findings, this study highlighted the importance of understanding how older adults use smart speakers and the practices through which they integrate them into their daily routines. The findings suggest that smart speakers can provide significant benefits for older adults, including increased convenience and improved quality of life. However, to promote maintainable use behaviors, designers and developers should consider more about the technology use contexts and the specific needs and preferences of older adults when designing these devices.
Effectiveness of a Nursing Educational Intervention in Adults to Promote Control Behaviors Against Dengue: Protocol for a Randomized Controlled Trial
Authors: Llorente Pérez Yolima Judith , Rodríguez-Acelas Alba Luz , Mattiello Rita , Cañon-Montañez Wilson 
Published: January 2024

Background
The increase in dengue cases can be attributed to social, demographic, environmental changes, or community-driven factors. In this regard, different strategies have been established in health education, using educational interventions as necessary tools for the reduction of the disease with the aim of reinforcing and stimulating the prevention and control of dengue.

Objective
This study aims to evaluate the effectiveness of a nursing educational intervention for dengue control.

Methods
A randomized controlled trial will be conducted with adults living in rural areas and participating in health promotion and disease prevention programs. We will enroll 116 adults. Adults will be randomized 1:1, with 58 adults assigned to the educational intervention group and 58 to the usual care group. Participants will receive 4 sessions over the course of a month, 1 week apart, and will be followed up for 1 month after the end of the educational intervention. Nursing Outcome Classification labels will be used to measure the outcomes: risk control (1902) and participation in health care decisions (1606).

Results
The participants in the intervention group are expected to achieve better dengue control behaviors than those in the usual care group.

Conclusions
Risk factors are fostered by the community, largely caused by artificial reservoirs or unprotected tanks in homes; also, the lack of information hinders the identification of symptomatology and the poor implementation of effective measures, and the development of standardized educational strategies can contribute to efficient and cost-effective control of the disease.

Trial Registration
ClinicalTrials.gov NCT05321264; https://clinicaltrials.gov/study/NCT05321264

International Registered Report Identifier (IRRID)
PRR1-10.2196/54286