bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health risks assessment for trace elements accumulation in Amaranthaceae family cultivars and their correlation with macro-minerals
Authors: Sharma Pooja , Mahongnao Sophayo , Nanda Sarita 
Published: January 2025

Abstract

The Yamuna Floodplain, a vital agricultural hub feeding Delhi’s growing population, faces significant challenges from soil contamination and unsustainable farming practices. In a quest for solutions that prioritize both crop quality and environmental health, this study explores the transformative potential of organic waste compost. Spinach, green amaranth, and red amaranth were cultivated in two distinct soils amended with compost, irrigated with groundwater, and rigorously assessed for their nutrient content and safety. Using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), we observed that compost amendments significantly boosted the concentrations of essential macro-minerals like phosphorus, sulfur, magnesium, and potassium, enhancing their contribution to Recommended Dietary Allowance (RDA) and Estimated Average Requirement (EAR) levels compared to chemical fertilizers. Health risk assessments revealed minimal non-carcinogenic risks, with hazard quotient (HQ) and health index (sum of Target Hazard Quotients, THQ) values well below 1. Moreover, compost amendments reduced risks associated with trace elements, including manganese, iron, copper, zinc, and selenium, while revealing interesting correlations between trace and macro-minerals. This study highlights urban organic compost as a sustainable soil amendment for the Yamuna Floodplain, promoting the production of high-quality, nutrient-rich vegetables with minimal health risks. These findings pave the way for environmentally friendly and health-conscious agricultural practices in a critical region.
Microbiome re-colonization dynamics of a moving bed biofilter in an Atlantic salmon recirculating aquaculture system.
Authors: Coca Yoandy , Sáez-Navarrete César , Caro Diego , Suárez Rudy , Bledsoe Jacob W. , Abanikannda Mosope F. , de Oca Marco Montes , Pontigo Juan Pablo , Rodríguez-Córdova Leonardo , Burbulis Ian , Kibenge Frederick S. B. , Godoy Marcos 
Published: January 2025

Abstract

Bacterial outbreaks commonly challenge fish health in recirculating aquaculture systems (RAS). Many practitioners believe that recycled biofilters may be a potential source of pathogen contamination during subsequent cycles of fish growth. Biofilters undergo rigorous chemical disinfection protocols, known as “knockout” procedures, as a precaution to neutralize the carry-over of infectious agents. However, the impact of these disinfection protocols on the re-establishment of nitrifying bacterial biofilms within plastic biofilter carriers remains unknown. In this study, a moving bed biofilter from a recirculating aquaculture system (RAS) was used over time during a complete production cycle of Atlantic salmon (Salmo salar) smolts, before their transfer to seawater. The analysis employed next-generation sequencing of 16S ribosomal RNA gene amplicons, alpha diversity metrics, and their correlation with fish health and water quality. Sampling was conducted every 15 d to collect data from biofilter carriers and the biofilter water. Our findings revealed that the “knockout” protocols affected the composition and abundance of nitrifying bacterial communities within the biofilter carriers. Contrary to the logic supporting aggressive “knockout” procedures, significant out-growth of freshwater bacterial pathogens, e.g., Aeromonas salmonicida, was not observed in the biofilter carriers when the RAS was cleaned using low salt conditions. Our findings corroborate those of previous reports showing that low salinity (2–5‰) may influence bacterial diversity and abundance during biofilm regrowth. Our findings suggest that aggressive disinfection may be unnecessary for safe and functional biofilm re-establishment in RAS.
Porphyromonas gingivalis levels correlate with Toll-like receptor 4 expression status and prognosis after esophagectomy for advanced thoracic esophageal squamous cell carcinoma
Authors: Yoneya Takatoshi , Sato Yusuke , Nomura Kyoko , Wakita Akiyuki , Nagaki Yushi , Sasamori Ryohei , Sasaki Yoshihiro , Nozaki Shu , Takahashi Tsukasa , Yamada Munehiro , Takahashi Mayu , Terata Kaori , Imai Kazuhiro 
Published: January 2025

Abstract

Background. Poor oral health is an independent risk factor for upper-aerodigestive tract cancers, including esophageal squamous cell carcinoma (ESCC). Porphyromonas gingivalis (P. gingivalis), the most studied periodontal pathogen, is a Gram-negative bacterium with walls composed of lipopolysaccharide (LPS). Toll-like receptor 4 (TLR4) recognizes LPS, and we previously reported that ESCC patients exhibiting high TLR4 expression also show significantly poorer survival than patients expressing lower levels of TLR4. We therefore hypothesized that P. gingivalis affects disease progression and prognosis in patients with ESCC.
Patients and Methods. We used an ESCC tissue microarray to assess the P. gingivalis status of ESCC patients and to determine whether P. gingivalislevels correlate with prognosis after curative esophagectomy. We also tested whether the combined P. gingivalisand TLR4 statuses correlate with prognosis in these patients.
Results. Clinical ESCC samples from 175 patients were tested for P. gingivalis. High P. gingivalis levels (3+ and 2+) correlated with significantly poorer 5-year overall survival (OS) and disease-specific survival (DSS) after esophagectomy than lower P. gingivalis levels (1+ and 0). Univariate and multivariate analyses showed that the P. gingivalis status is an independent prognostic factor affecting 5-year OS and DSS. Moreover, the combined P. gingivalis and TLR4 statuses are also an independent prognostic factor affecting 5-year OS and DSS.
Conclusion. P. gingivalis infection leads to TLR4 expression and a poorer prognosis in ESCC patients after esophagectomy. Preventing P. gingivalis infection may be an effective strategy for improving the long-term outcomes of patients with ESCC.
Somatic Genomic and Transcriptomic Changes in Single Ischemic Human Heart Cardiomyocytes
Authors: Choudhury Sangita , Hilal Nazia , An Zheming , Prondzynski Maksymilian , Matsui Erica , Sahu Debesh , Mao Shulin , Jung Youngsook , Yang Yingxi , Epstein Sonia , Chen Ming , Pu William , Monte Federica del , Huang August 
Published: January 2025

Abstract

Heart failure is a multifaceted syndrome contributing significantly to mortality and hospitalization rates among the global population1. One of the prevalent causes of heart failure is ischemic heart disease (IHD), often caused by a blockage in a coronary artery, ultimately leading to the loss of myocardial tissue and contractile force2. The impact of this ischemic ambiance on the cardiomyocyte genome and transcriptome has not been thoroughly studied. During normal aging, cardiomyocytes progressively accumulate somatic mutations faster than many dividing cells, suggesting that internal and external factors specific to cardiomyocytes might influence this accumulation3. In this study, we analyzed single-cell whole-genome and transcriptome data from the left ventricle of 5 individuals with IHD and 10 healthy control individuals. We found that somatic DNA alterations significantly increase in IHD cardiomyocytes, with distinct mutational patterns indicating a disrupted DNA repair system and a cytotoxic environment, potentially associated with increased inflammatory response in the myocardium and a compensatory anti-inflammatory response in IHD. An in vitro iPS-derived hypoxic cardiomyocyte mutational profile indicates similar mutational spectra. Transcriptomic analysis revealed increased expression of EGR1, FOS, and collagen genes in ischemic heart cardiomyocytes, leading to a more fibrotic heart. The aberrant accumulation of DNA alterations and changes in transcriptional patterns in the ischemic heart cardiomyocytes provide insight into the development of IHD.
Burden of Pulmonary Arterial Hypertension in China from 1990 to 2021, with Projections to 2050: An Analysis of Data from the Global Burden of Disease Study 2021
Authors: Wei Shuoshuo , Han Yonghui , Liu Min , Wang Hanli , Lu Zhiwei , Cheng Yusheng , Guo Jun , Zha Lei 
Published: January 2025

Abstract

Background
Pulmonary arterial hypertension (PAH) is a severe condition with high morbidity and mortality, yet its epidemiology in China remains poorly understood. This study aims to assess the burden of PAH in China from 1990 to 2021 and project trends to 2050, providing insights for effective management and prevention strategies.
Method
Data from the Global Burden of Disease (GBD) Study 2021 were analyzed, focusing exclusively on PAH. Key metrics included incidence, prevalence, mortality, and disability-adjusted life years (DALYs), along with their corresponding age-standardized rates (ASRs). Average annual percent changes (AAPCs) were calculated using joinpoint regression analysis, and a Bayesian age-period-cohort model was employed to project trends through 2050.
Results
In 2021, there were 41,135 estimated prevalent cases of PAH (95% UI: 32,838.91 to 51,357.25) in China, with an age-standardized prevalence rate (ASPR) of 2.24 per 100,000. Females accounted for approximately 58% of cases. PAH caused 7,318 deaths (95% UI: 4,835.72 to 9,075.75; 3,683 males, 3,635 females), with an age-standardized death rate (ASDR) of 0.42 per 100,000. From 1990 to 2021, the ASPR increased slightly from 2.07 (95% UI: 1.68 to 2.54) to 2.24 (95% UI: 1.81 to 2.75), while the ASDR decreased from 0.61 (95% UI: 0.46 to 0.83) to 0.42 (95% UI: 0.28 to 0.51). Projections suggest declining trends in both ASPR and ASDR, although the overall prevalence of PAH is expected to rise over the next 30 years.
Conclusion
PAH imposes a significant disease burden in China, particularly among women and older adults. These findings highlight the urgent need to enhance diagnostic capabilities and develop improved treatment strategies to address this challenging condition in the Chinese population.
CALLY Index as a Comprehensive Biomarker for Heart Failure Risk: Findings from NHANES 1999–2010
Authors: Yang Boning , Zou Junhua , Zhou Run , Wang Anqi , Hu Yuchi , Wang Jing 
Published: January 2025

Abstract

Background
Heart failure (HF) is a critical global health issue characterized by high morbidity, mortality, and economic burden. The interplay of chronic inflammation, malnutrition, and immune dysregulation is central to HF pathogenesis. The C-reactive protein-Albumin-Lymphocyte (CALLY) index, a composite biomarker that integrates inflammatory, nutritional, and immune parameters, offers a novel, holistic approach to HF risk prediction. However, its association with HF prevalence and potential clinical utility remains underexplored.
Methods
This study utilized data from the NHANES 1999–2010 cohort, encompassing 14,900 participants after stringent inclusion criteria. The CALLY index was computed as albumin (g/L) × lymphocyte count (10⁹/L) / C-reactive protein (CRP, mg/L). Logistic regression models were utilized to assess the relationship between CALLY index quartiles and HF prevalence, with adjustments made for demographic, socioeconomic, and clinical factors. Nonlinear associations were examined using restricted cubic spline analysis, while subgroup analyses evaluated differences across age, gender, and ethnicity.
Results
Participants with HF (n = 659, 4.4%) exhibited significantly lower CALLY index values compared to those without HF. Multivariate analyses revealed a robust, dose-dependent association between higher CALLY index quartiles and HF risk (Q4 vs. Q1: adjusted OR = 7.49, 95% CI: 5.89–9.52, P < 0.001). Restricted cubic spline analysis identified a threshold value of 692, above which HF risk sharply increased. Subgroup analyses highlighted significant heterogeneity, with stronger associations observed among older adults (≥ 60 years), males, and non-Hispanic Black populations, suggesting demographic-specific predictive utility.
Conclusions
The CALLY index serves as a low-cost, readily accessible biomarker for HF risk stratification, integrating critical dimensions of inflammation, nutrition, and immune function. Its nonlinear association with HF risk and identified threshold offer practical insights for early detection and intervention. Tailored strategies for high-risk demographic groups, such as older adults and non-Hispanic Black individuals, could enhance its clinical application. Further longitudinal studies and interventional trials are required to confirm these findings and evaluate the broader applicability of the CALLY index in cardiovascular risk management.
Targeted downregulation of FIS1 in excitatory neurons within the spinal dorsal horn alleviates neuropathic pain through the mitigation of mitochondrial fragmentation
Authors: Zhu Chang-Lei , Li Shu-Jiao , Lin Zhi-Peng , Ni Zi-Wei , Tian Ke , Xia Yu-Lu , Tie Jing-Jing , Pu Xue-Yin , Huang Yun-Qiang , Wu Fei-Fei , Liu Hui , Zhang Kun-Long , Zhang Shuai , Wu You-Sheng , Tian Fei , Liu Nan-Nan , Yang Yan-Ling , Wang Ya-Yun 
Published: January 2025

Abstract

Background: Neuropathic pain has been shown to induce abnormal mitochondrial fission in neurons, yet the analgesic potential of inhibiting this process remains unclear. Our previous studies demonstrated that targeted regulation of the dynamin-related protein (DRP1) can alleviate neuropathic pain; however, the downstream molecular signaling mechanisms remain to be elucidated.
Methods: To investigate the role of mitochondrial dynamics in neuropathic pain, we utilized C57BL/6J mice, GAD2-Cre mice, and vGluT2-Cre mice. Mitochondrial network changes in pain states were assessed using GAD2-MITO and vGluT2-MITO transgenic mouse models combined with MiNA analysis. Pain thresholds and the expression levels of various molecules in the spinal dorsal horn (SDH) were evaluated through behavioral tests, immunofluorescence, and Western blotting. Mitochondrial morphology and function in pain conditions were examined using electron microscopy, mitochondrial membrane potential, reactive oxygen species, and adenosine triphosphate assays. The effects of antioxidant analgesics epigallocatechin gallate (EGCG) and Cinnamic Acid on molecular changes in the SDH during pain states were also investigated.
Results: We observed that mitochondrial networks in both excitatory and inhibitory neurons of the SDH were disrupted in spared nerve injury mice, as evidenced by GAD2-MITO and vGluT2-MITO transgenic mouse models. Specifically, down-regulating FIS1 in excitatory neurons, but not in inhibitory neurons, within the SDH elicited analgesic effects, as evidenced by experiments conducted with vGluT2-Cre and GAD2-Cre mouse models. Additionally, epigallocatechin gallate (EGCG), which effectively down-regulates FIS1 in the SDH, concurrently inhibited SNI-induced neuropathic pain. These findings suggest that reducing mitochondrial fragmentation by down-regulating FIS1 in SDH excitatory neurons can alleviate neuropathic pain.
The clinical efficacy of unilateral biportal endoscopic treatment for lumbar spinal stenosis with preservation of the ligamentum flavum
Authors: Li Kepeng , Jiang Bo , Han Ye 
Published: January 2025

Abstract

Background
As a minimally invasive surgical approach for treating lumbar spinal stenosis, Unilateral Biportal Endoscopy (UBE) is receiving significant attention from clinical practitioners. Conventional UBE surgery involves the removal of the ligamentum flavum, and to date, there is no research on UBE techniques that preserve the ligamentum flavum.
Method
A retrospective analysis was conducted on 100 patients with lumbar spinal stenosis (LSS) who underwent UBE treatment from January 2022 to March 2024. The surgical techniques of decompression of the lumbar canal while preserving the ligamentum flavum using UBE technology were summarized. Patients were divided into two groups based on whether the ligamentum flavum was preserved during surgery: the preservation group with 40 patients, and the removal group with 60 patients. The intraoperative surgical time and blood loss of the two groups were compared, as well as perioperative Visual Analogue Pain Scores (VAS), Oswestry Disability Index (ODI), and complications such as dural tears and epidural hematoma. The difference in radiological decompression between the two groups was assessed using the cross-sectional area of the dural sac on lumbar MRI.
Results
The average surgery time for the group with the ligamentum flavum preserved was (50.13±8.45) minutes, and the intraoperative blood loss was (30.57±6.64) ml. For the group with the ligamentum flavum removed, the average surgery time was (66.47±7.26) minutes, and the intraoperative blood loss was (58.70±6.19) ml. Compared to the group with the ligamentum flavum removed, the group with the ligamentum flavum preserved had a shorter surgery time and less blood loss, with these results being statistically significant (P < 0.01). There was no difference in the VAS scores and ODI scores between the two groups at 3 days and 6 months postoperatively. The postoperative dural sac cross-sectional area for the ligamentum flavum preserved group and the ligamentum flavum removed group was 164.31±26.35 mm²and 170.92±27.68 mm², respectively, with no statistically significant difference (P = 0.24). The incidence of postoperative complications was 1.92% in the ligamentum flavum preserved group and 5.77% in the ligamentum flavum removed group, which was not statistically significant (P = 0.61).
Conclusion
The technique of unilateral biportal endoscopy (UBE) for lumbar spinal stenosis, which preserves the ligamentum flavum, can reduce complications, surgical time, and blood loss, while simultaneously providing favorable surgical outcomes. It is recommended to preserve the ligamentum flavum as much as possible when using UBE for the treatment of patients with lumbar spinal stenosis.
Pure Laparoscopic Treatment of a Rare Giant Post-Mesenteric Benign Ovarian Seromucinous Cystadenoma: A Case Report and Literature Review
Authors: Ye Yifan , Liu He , Huang Rui , Li NingBo , Tang Yaling , Chen Hong 
Published: January 2025

Abstract

Background
Ovarian seromucinous tumors, although relatively rare, represent a distinctive subset of ovarian neoplasms. These tumors are predominantly benign but carry a potential risk of malignant transformation over time. Early detection and management are crucial for improving outcomes. For large or complex masses, open surgery is preferred for better access and intraoperative histological assessment. However, with the advancement of laparoscopic techniques, minimally invasive surgery has emerged as a viable alternative for select patients, offering comparable efficacy, along with the advantages of reduced recovery times and minimal scarring.
Case report:
A 65-year-old female presented with a seven-month history of abdominal distension, a sensation of fullness beneath the xiphoid, left-sided discomfort, and intermittent morning cramping. Laboratory findings revealed a mild elevation in CA-125 to 46 U/mL, and CT imaging suggested a diagnosis of an ovarian cystadenoma or possibly a retroperitoneal mass. Preoperative assessment was complicated by the tumor's irregular shape, large size, and adhesions to nearby structures, making its origin unclear. A single-port laparoscopic approach was selected to minimize tissue damage, improve tumor handling, and reduce risks of fluid leakage or tumor spread. Postoperative pathology confirmed the lesion as a seromucinous ovarian cystadenoma.
Conclusion
This case emphasizes the need for a multidisciplinary approach in tumor diagnosis and treatment, highlighting the benefits of minimally invasive surgical techniques for better patient outcomes. Given the rarity of ovarian seromucinous tumors, continued research into their pathogenesis, classification, and treatment strategies is essential for advancing our understanding and optimizing management approaches for patients affected by these tumors.
Deep Learning Applications in the Resilience Critical Infrastructure Systems—A Systematic Review
Authors: Gururaj H L 
Published: January 2025

Abstract

Technological advancements like AI, blockchain, and IoT are merging to bring about a new level of digital change. Critical infrastructure systems (CISs) are vital to modern society, as they support crucial social functions, economic organization, and national defense. Recently, the resilience of CISs has garnered attention in academic and policy fields, particularly in light of increased natural and technological disasters. However, assessing CIS resilience remains challenging, particularly in its practical application to operational risk management. Integrating advanced technologies with critical infrastructure (CI) can significantly enhance the quality of life and boost national economic productivity. Nevertheless, the lack of robust cybersecurity in CI has given rise to advanced threats and vulnerabilities, undermining these potential benefits. The paper explores cyber vulnerabilities and dangers in various critical structures, including the financial, agricultural, energy, and health systems. Moreover, we examine the positive aspects of artificial intelligence and provide a rich taxonomy of solutions that show how well AI-based approaches deal with different types of cyberattacks on critical infrastructure.
Melatonin reduces cell motility and antioxidant defenses in ovarian cancer cell lines
Authors: Silveira Henrique Spaulonci , Cesário Roberta Carvalho , Simão Vinicius Augusto , Guimarães Fernando , Seiva Fábio Rodrigues Ferreira , Zuccari Debora Aparecida P. C. , Fernandes Glaura Scantamburlo Alves , de Souza Milena Cremer , Reiter Russel J. , Chuffa Luiz Gustavo de Almeida 
Published: January 2025

Abstract

Ovarian cancer (OC), a highly recurrent and fatal tumor, poses diagnostic challenges due to generic symptoms and chemoresistance. Melatonin (Mel) is an indoleamine acting against tumor progression and exhibiting pro-oxidative actions in tumor cells. This study explores the impact of Mel on antioxidant defenses of OC cells (SKOV-3 and CAISMOV-24 lines), focusing on its receptor-dependent and -independent effects. Cell viability was assessed using the MTT method and the antioxidant system was analyzed by preparing supernatants for assessing glutathione (GS), reduced glutathione (GSH), oxidized glutathione (GSSG), catalase (CAT), glutathione S-transferase (GST), and superoxide dismutase (SOD). Mel stimulated its own intracellular levels, reducing cell viability in both cell lines. Notably, Mel independently of its membrane receptors, inhibited migration and invasion, thus showing its anti-tumoral potential. By investigating melatonin’s actions, we observed an impact on the antioxidant system primarily through the reduced activity of CAT and the GS axis. The modulation of these antioxidants by Mel demonstrates its multifaceted role in OC, emphasizing its therapeutic potential. We also demonstrated, for the first time, the theoretical ability of Mel to bind to CAT, which may be responsible for the reduction in enzyme activity. This study contributes with novel insights into Mel's receptor-independent actions, providing a foundation for further research in OC therapy.