bluuebunny/crossref_metadata_2025_split huggingface dataset:
Graduate Nurses in Acute Mental Health Settings and Their Viewpoints of Nursing Education to Support Their Transition to Mental Health Nursing: A Q Methodology Study
Authors: Lim Eric , Higham Kezia , Ferraz Kristina , Harban Kathryn , Tate Rebecca , Sewell Amy , Murdock Jane , Delahunty Sharon 
Published: January 2025

ABSTRACTGraduate nurses are now comprehensively prepared in Australia and have limited mental health knowledge and experiences to work in acute mental health settings. As such, graduate nurses would need the support that they can get from their respective mental health services to progress from novice to advanced beginner. Nursing education is an important support for graduate nurses to develop the knowledge and skills required for them to transition successfully into acute mental health settings. This study explored 12 graduate nurses' experiences with the nursing education of one mental health services in Western Australia. Q methodology was the approach chosen to conduct this study as it is a ‘ready‐to‐use’ mixed method research approach that can enable the researchers to obtain in‐depth and accurate understanding of the researched topic. Data analysis generated four factors which revealed that nursing education needs to: (1) be tailored to the area of speciality for graduate nurses who experience challenges of being accepted by their nursing team; (2) provide advanced specialist mental health training for graduate nurses who have low self‐confidence about their mental health nursing knowledge and skills to practice in acute mental health settings; (3) provide clinical supervision for graduate nurses who experience challenges to apply their mental health knowledge and skills to difficult clinical situations; and (4) change the way mental health education and training are delivered for graduate nurses who feel confident with their mental health knowledge and skills and accepted by their nursing team. The findings of this study highlighted the usefulness of Q methodology in nursing research. The findings of this study can be used by nurse educators to provide more individualised education and training for graduate nurses. Future research will be conducted to test the use of different teaching and learning approaches for the different categories of graduate nurses in acute mental health settings and develop evidence‐based nursing education.
Exploring Students' Perceptions and Experiences of Raising Concerns During Pre‐Registration Training in England: A Systematic Review
Authors: Phillips Charlotte , Smith Hazel Ann , Crisp Elizabeth , Carter Vanda , Donnelly Helene , Ross Al , Bowie Paul , Latham Callum , Jones Sarahjane 
Published: January 2025

ABSTRACTAimTo explore the perceptions and experiences of students raising concerns during pre‐registration health and/or social care training in England.DesignSystematic review.Data SourcesMEDLINE, CINAHL, ERIC, PsycINFO and Education Research Complete were systematically searched for studies published between September 2015 and August 2024. Grey literature searches were conducted using Google Scholar and ETHOS British Library. Reference lists from included studies were hand searched.MethodsJoanna Briggs Institute methodological guidance for the conduct of systematic review informed conduct and the convergent integrated approach. Mixed methods appraisal tool was used for quality appraisal.FindingsEleven studies were included. Synthesis of findings generated three themes: (1) conflicting needs of self and others, (2) navigating the professional workspace and, (3) ‘choice to voice’.ConclusionSpeaking up and raising concerns as a pre‐registration student is a complex, multi‐faceted and non‐linear social phenomenon. Experiences and perceptions are impacted by the novice student position alongside individual, interpersonal and organisational factors. Open cultures within teams and organisations, leadership, support and feedback may enable students overcome barriers to raising concerns.ImpactRaising concerns may reduce avoidable harm. Pre‐registration students offer a ‘fresh pair of eyes’; however, they face barriers related to their student position. Synthesis of speaking‐up experiences and perceptions of students in English settings can inform the design of learning environments which equip pre‐registration students with the knowledge and skills required to cultivate safety behaviours. These skills contribute positively to safety culture and support learning and improvement in complex systems such as health and social care.Reporting MethodThe review followed PRISMA reporting guidelines.Patient or Public ContributionThe conceptualisation of this project was informed by engagement events with higher education staff, students and Freedom to Speak Up Guardians.
Adaptation and Validation of the Supportive and Palliative Care Indicators Tool (SPICT): A Quantitative Methodological Study
Authors: Huang Yufei , Deng Cunqing , Lin Haiyang , Peng Meifang , Hao Yanping 
Published: January 2025

ABSTRACTAimsTo translate the Supportive and Palliative Care Indicators Tool (SPICT) into Chinese and conduct preliminarily tests of its performance in hospitalized patients with cancer.DesignA cross‐sectional validation study conducted from January to March 2024.MethodsSPICT 2022 was translated in both directions, following the Brislin translation model, and the Chinese version culturally debugged through expert consultation and pre‐testing. Content validity was evaluated by expert scoring. Tool internal consistency was evaluated using KR‐20 coefficient, and retest reliability was evaluated using kappa coefficient. The screening performance was evaluated by sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV).ResultsOf 388 hospitalized cancer patients included, approximately one‐quarter had potential palliative care needs. Content validity of the Chinese version of SPICT was good, as were internal consistency and test–retest reliability. Accuracy (0.905), sensitivity (0.806), specificity (0.943), PPV (0.845), and NPV (0.926) for the Chinese version of SPICT indicated that it is an acceptable instrument.ConclusionThe Chinese version of SPICT can be applied for screening of palliative care needs in hospitalized patients with cancer in China.Implications for the Profession and/or Patient CareThe Chinese version of SPICT had been adapted to assist clinicians or nurses in quickly identifying hospitalized patients with cancer who may have palliative care needs. This is conducive to help clinical team to start palliative care consultation, care goal discussion and (or) referral for patients in clinical practice. And it probably helps to advance integration between palliative care assessment and routine oncology care assessment.ImpactThis study provided a screening tool for palliative care, with good validity and reliability, as well as excellent screening performance to facilitate palliative care need screening in clinical practice, promote palliative care referrals and improve patient quality of life.Reporting MethodThis study was reported according to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement.Patient or Public ContributionNo patient or public contribution.
Boswellia Extract Promotes Healing and Resolving Inflammation in Oral Ulcers of Rat
Authors: Zhao Wei , Jia Zhuoqun , Han Jiao , Sun Xiaojun 
Published: January 2025

ABSTRACTBackgroundRecurrent aphthous ulcers significantly impact patients' quality of life due to their painful and recurrent nature, necessitating more effective treatments. This study explores the therapeutic potential of Boswellia to treat recurrent aphthous ulcers by its anti‐inflammatory and healing promotion effect in a rat oral ulcer model.MethodsNetwork pharmacology techniques were employed to elucidate Boswellia's active components and potential targets. Intersecting targets of Boswellia and oral ulcer‐related genes were screened for protein–protein interaction network analysis and functional enrichment. An oral ulcer model in rats was used and rats were treated with Boswellia extract. The healing process was monitored by measuring the ulcer area and body weight changes. Histological analysis was performed, and the role of Boswellia in macrophage polarization was investigated through gene expression analysis and protein array tests. The underlying mechanism involving PPARγ activation was also explored.ResultsNetwork pharmacology analysis revealed Boswellia's interaction with key genes and pathways associated with inflammation and lipid metabolism, such as MAPK3, PPARG, and PTGS2. Boswellia extract significantly accelerated oral ulcer healing and recovered weight loss in rats. Histological examinations revealed reduced tissue swelling and inflammatory cell infiltration in treated groups. Furthermore, Boswellia extract decreased infiltration of M1 macrophage presence while increasing M2 macrophage, indicating an inflammation‐resolving effect. In vitro studies showed that Boswellia extract enhanced M2‐related gene expression and decreased pro‐inflammatory cytokines, which is PPARγ dependent.ConclusionBoswellia extract promotes oral ulcer healing and resolves inflammation, primarily through the modulation of macrophage polarization via PPARγ activation.
Friends' influence on Adolescents' loss of control eating: The moderating role of self‐esteem, fear of negative evaluation, and body dissatisfaction
Authors: Beckers Desi , Burk William J. , Larsen Junilla K. , Cillessen Antonius H. N. 
Published: January 2025

AbstractFriends' influence may importantly contribute to the development of adolescent disordered eating behaviors. However, little is known about the influence of friends on loss of control eating. This study investigated whether friend‐reported loss of control eating was associated with changes in adolescents' own loss of control eating 1 year later and tested whether adolescents with lower self‐esteem, higher fear of negative evaluation, and higher body dissatisfaction were more susceptible to friends' influence. Sex differences in these associations were also examined. Participants were 612 adolescents (50.0% female; MT1 Age = 13.50 years, SDT1 Age = 1.01) who were recruited from a school in the Netherlands with predominantly native Dutch students and participated in an ongoing longitudinal research project between 2019 and 2021. Results indicated that adolescents and their friends reported similar levels of loss of control eating but provided no support for friends' influence on loss of control eating, nor for differential susceptibility. The modest degree of similarity between adolescents' and their friends' loss of control eating and the lack of friends' influence on loss of control eating may be due to homophilic selection effects or the restrictions involving the COVID‐19 pandemic, so replication of the results is warranted.
Educational or Behavioural Interventions to Improve Long‐Term Haemodialysis Vascular Access Self‐Management: A Systematic Review
Authors: Wembenyui Colette , Marsh Nicole , Larsen Emily , Bonner Ann 
Published: January 2025

ABSTRACTBackgroundGlobally, haemodialysis is the most frequent type of kidney replacement therapy and necessitates access to the bloodstream either through a native arteriovenous fistula, arteriovenous graft or central venous catheter. Vascular access complications are a major cause of morbidity and mortality in adults receiving haemodialysis, and effective vascular access self‐management is required.ObjectiveTo examine the effectiveness of educational or behavioural interventions designed to improve self‐management of long‐term vascular access in adults receiving haemodialysis.DesignSystematic review.MethodEnglish language publications from January 2013 to May 2023 retrieved from PubMed, Embase, CINAHL, Cochrane Library, PsycINFO and Joanna Briggs Institute (JBI) databases were undertaken. Two independent reviewers identified studies for full‐text review, data extraction and quality assessment. Data synthesis and quality assessment followed the JBI guidelines for quantitative reviews.ResultsSeven studies involving 540 participants were included: two studies were randomised control trials and five were quasiexperimental. All studies involved patient education, predominantly provided by nurses, and employed a variety of teaching resources, such as education booklets, practical demonstrations and videos. Outcomes measured included vascular access self‐management behaviours, self‐efficacy and vascular access knowledge although there was no consistency between studies. Overall, vascular access self‐management significantly improved following education.ConclusionEducational interventions led to improvements in self‐management behaviours in adults with long‐term haemodialysis vascular access. However, there was insufficient evidence for the delivery and duration of intervention education. Further research is needed. An evidence‐based nurse‐led codesign intervention could lead to improvements in vascular access self‐management.
Investigating the influence of blood meal sources on the composition of culturable haemolytic gut bacteria of a wild‐caught BTV vector Culicoides oxystoma Kieffer (Diptera: Ceratopogonidae)
Authors: Sarkar Ankita , Banerjee Paramita , Kar Surajit , Pal Arjun , Mazumdar Abhijit 
Published: January 2025

AbstractCulicoides oxystoma Kieffer (Diptera: Ceratopogonidae) transmits many pathogens, including seven viruses, four protozoa and one nematode. This species has a wide distribution range across northern Afro‐tropical, Palearctic, Australian, Indo‐Malayan realms with a broad host spectrum, including cattle, buffaloes, sheep, pigs, dogs, horses and even humans. The heterogeneous nature of Culicoides' blood‐feeding patterns is well documented, but the influence of various host blood meal sources on gut bacterial composition remains scant. Adult midges were collected during April (2023) by operating UV light traps in cattle, buffalo sheds and poultry farm in Purulia (India). Besides C. oxystoma, eleven Culicoides species were collected across the sheds and farm, seven of which are vectors. Culicoides liui Wirth and Hubert and C. thurmanae Wirth and Hubert are reported from India for the first time. In all the sheds, engorged females of C. oxystoma were ubiquitous. Identification of culturable gut bacteria and the host blood meal of C. oxystoma were done through the polymerase chain reaction (PCR)‐based method. Blood meal analysis confirmed the following hosts: cattle, buffaloes and humans. Identification of blood meal of engorged C. oxystoma caught from poultry farm showed positive results for humans but not for birds. Among bacteria, Bacillus cereus was abundant in all of the engorged females. Bacillus paramycoides and Enterococcus faecium were identified from females feeding on cattle and buffaloes' blood, while Alcaligenes faecalis was found in the gut contents of females that fed on cattle and human blood. The gut bacteria Alcaligenes faecalis exhibited alpha haemolytic activity. In contrast, Bacillus sp., B. cereus, B. flexus, B. licheniformis, B. thuringiensis, B. paramycoides, E. faecium, Paenibacillus sp. and Pseudomonas sp. exhibited beta haemolysis. This is the first report on the composition of gut bacteria, with particular emphasis on the haemolytic bacteria of C. oxystoma with different host blood meals. The pathogenic bacteria B. cereus, B. licheniformis and A. faecalis within the females could potentially impact pathogen acquisition and increase the probability of their zoonotic transmissibility.
A Study on the Effects of Gluteal Muscle Activation on the Electromyography of Lower Limb Muscles in Young Male Patients With Patellofemoral Pain Syndrome
Authors: Yue Wu , Shuang Ren , Hongshi Huang , Yingfang Ao , Bo Gou 
Published: January 2025

ABSTRACTObjectivePatellofemoral pain syndrome (PFPS) is a common knee issue, and hip joint function significantly affects knee health. Gluteus activation exercises are a promising treatment for PFPS. This study aims to investigate the impact of gluteal muscle activation exercises on the muscle involvement and movement patterns of young male patients with PFPS.MethodsOur study was a randomized controlled clinical trial study from June 2020 to December 2021, included 18 young male patients with PFPS, randomly divided into two groups: the gluteus activation group (GAG) and control group (CON), with nine cases in each group. The GAG underwent gluteal muscle activation exercises for 40 min per session, three times per week, for 6 weeks; the CON received no intervention. At baseline and after 6 weeks, the integrated electromyography (IEMG), contribution rates (CRs), and activation times (ATs) of the gluteus maximus (GM), vastus medialis (VM), rectus femoris (RF), vastus lateralis (VL), biceps femoris (BF), and semitendinosus (ST) muscles of the affected lower limb during stair‐climbing exercise were assessed. Additionally, the explosive power (EP) of the lower limbs and the visual analog scale (VAS) pain value of the knee joint were evaluated. Paired sample t‐tests and independent sample t‐tests were used to compare the differences within and between groups.ResultsAfter 6 weeks, the GAG showed a significant increase in the IEMG of GM by 118 ± 67.09 μVs compared to CON (p < 0.05), and an increase in the CR of the GM by 6.75% (p < 0.05). Additionally, the AT of the GM and BF was significantly reduced (p < 0.05), and the lower limb EP increased by 14.66% compared to the CON (p < 0.05). Concurrently, there was a very significant reduction in the knee VAS pain score (p < 0.01). The CON exhibited no significant changes in the EMG indices of the lower limbs, EP, and VAS before and after the 6 weeks (p > 0.05).ConclusionA 6‐week gluteal muscle activation training program for patients with PFPS can adjust and optimize the IEMG, CR, and firing order of the lower limb muscle groups, enhance EP, and alleviate pain.
Pediatric Transplant Surgeons' Perspectives on Palliative Care for Children With Chronic Kidney Disease: A National Cross‐Sectional Survey
Authors: House Taylor R. , Wightman Aaron , Smith Jodi , Dick Andre , Bradford Miranda C. , Rosenberg Abby R. 
Published: January 2025

ABSTRACTBackgroundSome adult transplant surgeons consider transplant to be contraindicated in patients receiving palliative care (PC). Little is known about pediatric transplant surgeons' attitudes toward PC. We sought to ascertain pediatric kidney transplant surgeons' perspectives regarding the routine integration of PC for children with chronic kidney disease.MethodWe administered a cross‐sectional web‐based survey to members of the American Society of Transplant Surgeons listserv in summer 2021. We adapted the survey from the previously validated Provider Survey about Palliative Care for Children with Heart Disease and pretested it with representative kidney transplant surgeons, nephrologists, and PC physicians; queries related to PC included institutional and personal experience, knowledge, and education. Data were summarized descriptively.ResultsThere were 21 participants. Over half of the respondents were white (57%) males (62%), practicing in urban, academic centers (94%). Although 67% of the participants practiced in an institution with a subspecialty PC team, 24% were unsure if such a team existed in their institution. A minority (19%) perceived PC consultation and kidney transplant to be mutually exclusive. Most surgeons (86%) believed that PC should not be restricted to when a child is dying, and 59% reported that PC consultation should happen at diagnosis for life‐threatening conditions. However, surgeons indicated that PC consultation is rarely utilized for pediatric kidney transplant recipients. Transplant surgeons expressed a desire for additional PC‐focused training and willingness to engage in additional education.ConclusionsAlthough a minority of pediatric transplant surgeons perceived PC to be contraindicated for kidney transplant, most indicated openness to PC engagement for their patients.
Female‐biased nectar production is associated with the Darwin's inflorescence syndrome
Authors: Strelin M. M. , Gavini S. S. , Aizen M. A. 
Published: January 2025

Abstract

Plant reproduction is influenced not only by individual flower characteristics but also by the arrangement of flowers within inflorescences. In bee‐pollinated plants with protandrous flowers in vertical acropetal inflorescences — where male fertile flower structures mature before female ones and basal flowers open first (i.e., Darwin's inflorescence configuration) — male‐phase flowers are positioned above female‐phase flowers. This arrangement benefits from the upward foraging motion of bees, reducing pollen transfer between flowers of the same plant and increasing pollen export. Additionally, female‐biased nectar production in these species could reinforce upward bee movement, further reducing self‐pollen transfer.

We conducted a comparative study of 112 hermaphrodite angiosperm species with temporal separation of male and female reproductive functions (i.e., dichogamy) to explore whether female‐biased nectar production is common among bee‐pollinated species with Darwin's inflorescence configuration.

Our results revealed female‐biased nectar production in bee‐pollinated species with Darwin's inflorescence configuration. In the absence of bee pollination, species with this type of inflorescence exhibit male‐biased nectar production. Gender‐biased nectar production is absent in species lacking this inflorescence type.

Female‐biased nectar production in vertical acropetal inflorescences with protandrous flowers likely evolved independently in different bee‐pollinated species to enhance pollen export. Our study emphasizes the need for microevolutionary research on how nectar production patterns adapt to bee pollination in species with Darwin's inflorescence configuration. It also calls for investigation into the developmental biases influencing nectar production in plants with dichogamous flowers.
MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering
Authors: Melero‐Cobo Xavier , Gallemí Marçal , Carnicer Marc , Monte Elena , Planas Antoni , Leivar Pablo 
Published: January 2025

AbstractPhotosynthetic microalgae are promising green cell factories for the sustainable production of high‐value chemicals and biopharmaceuticals. The chloroplast organelle is being developed as a chassis for synthetic biology as it contains its own genome (the plastome) and some interesting advantages, such as high recombinant protein titers and a diverse and dynamic metabolism. However, chloroplast engineering is currently hampered by the lack of standardized cloning tools and Design‐Build‐Test‐Learn workflows to ease genomic and metabolic engineering. The MoClo (Modular Cloning) toolkit based on Golden Gate assembly was recently developed in the model eukaryotic green microalgae Chlamydomonas reinhardtii to facilitate nuclear transformation and engineering. Here, we present MoCloro as an extension of the MoClo that allows chloroplast genome engineering. Briefly, a Golden Gate‐compatible chloroplast transformation vector (pWF.K.4) was constructed, which contains homologous arms for integration at the petA site in the plastome. A collection of standardized parts (promoters, terminators, reporter and selection marker genes) was created following the MoClo syntax to enable easy combinatorial assembly of multi‐cassettes in the destination pWF.K.4 vector. The functionality of the biobricks was assayed by constructing and assessing the expression of several multigenic constructs. Finally, a generic vector pK4 was constructed for easy Golden Gate cloning of 5′ and 3′ homologous arms, allowing targeting at alternative plastome integration sites. This work represents a significant advancement in technology aimed at facilitating more efficient and rapid chloroplast transformation and engineering of green microalgae.