bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-time imaging of mitochondrial redox reveals increased mitochondrial oxidative stress associated with amyloid β aggregates in vivo in a mouse model of Alzheimer’s disease
Authors: Calvo-Rodriguez Maria , Kharitonova Elizabeth K. , Snyder Austin C. , Hou Steven S. , Sanchez-Mico Maria Virtudes , Das Sudeshna , Fan Zhanyun , Shirani Hamid , Nilsson K. Peter R. , Serrano-Pozo Alberto , Bacskai Brian J. 
Published: January 2024

Abstract
Background
Reactive oxidative stress is a critical player in the amyloid beta (Aβ) toxicity that contributes to neurodegeneration in Alzheimer’s disease (AD). Damaged mitochondria are one of the main sources of reactive oxygen species and accumulate in Aβ plaque-associated dystrophic neurites in the AD brain. Although Aβ causes neuronal mitochondria reactive oxidative stress in vitro, this has never been directly observed in vivo in the living mouse brain. Here, we tested for the first time whether Aβ plaques and soluble Aβ oligomers induce mitochondrial oxidative stress in surrounding neurons in vivo, and whether this neurotoxic effect can be abrogated using mitochondrial-targeted antioxidants.

Methods
We expressed a genetically encoded fluorescent ratiometric mitochondria-targeted reporter of oxidative stress in mouse models of the disease and performed intravital multiphoton microscopy of neuronal mitochondria and Aβ plaques.

Results
For the first time, we demonstrated by direct observation in the living mouse brain exacerbated mitochondrial oxidative stress in neurons after both Aβ plaque deposition and direct application of soluble oligomeric Aβ onto the brain, and determined the most likely pathological sequence of events leading to oxidative stress in vivo. Oxidative stress could be inhibited by both blocking calcium influx into mitochondria and treating with the mitochondria-targeted antioxidant SS31. Remarkably, the latter ameliorated plaque-associated dystrophic neurites without impacting Aβ plaque burden.

Conclusions
Considering these results, combination of mitochondria-targeted compounds with other anti-amyloid beta or anti-tau therapies hold promise as neuroprotective drugs for the prevention and/or treatment of AD.
LINC00571 drives tricarboxylic acid cycle metabolism in triple-negative breast cancer through HNRNPK/ILF2/IDH2 axis
Authors: Xi Zihan , Huang Haohao , Hu Jin , Yu Yuanhang , Ma Xianxiong , Xu Ming , Ming Jie , Li Lei , Zhang Hui , Chen Hengyu , Huang Tao 
Published: January 2024

Abstract
Background
Triple-negative breast cancer is a complex breast malignancy subtype characterized by poor prognosis. The pursuit of effective therapeutic approaches for this subtype is considerably challenging. Notably, recent research has illuminated the key role of the tricarboxylic acid cycle in cancer metabolism and the complex landscape of tumor development. Concurrently, an emerging body of evidence underscores the noteworthy role that long non-coding RNAs play in the trajectory of breast cancer development. Despite this growing recognition, the exploration of whether long non-coding RNAs can influence breast cancer progression by modulating the tricarboxylic acid cycle has been limited. Moreover, the underlying mechanisms orchestrating these interactions have not been identified.

Methods
The expression levels of LINC00571 and IDH2 were determined through the analysis of the public TCGA dataset, transcriptome sequencing, qRT‒PCR, and Western blotting. The distribution of LINC00571 was assessed using RNA fluorescence in situ hybridization. Alterations in biological effects were evaluated using CCK-8, colony formation, EdU, cell cycle, and apoptosis assays and a tumor xenograft model. To elucidate the interaction between LINC00571, HNRNPK, and ILF2, RNA pull-down, mass spectrometry, coimmunoprecipitation, and RNA immunoprecipitation assays were performed. The impacts of LINC00571 and IDH2 on tricarboxylic acid cycle metabolites were investigated through measurements of the oxygen consumption rate and metabolite levels.

Results
This study revealed the complex interactions between a novel long non-coding RNA (LINC00571) and tricarboxylic acid cycle metabolism. We validated the tumor-promoting role of LINC00571. Mechanistically, LINC00571 facilitated the interaction between HNRNPK and ILF2, leading to reduced ubiquitination and degradation of ILF2, thereby stabilizing its expression. Furthermore, ILF2 acted as a transcription factor to enhance the expression of its downstream target gene IDH2.

Conclusions
Our study revealed that the LINC00571/HNRNPK/ILF2/IDH2 axis promoted the progression of triple-negative breast cancer by regulating tricarboxylic acid cycle metabolites. This discovery provides a novel theoretical foundation and new potential targets for the clinical treatment of triple-negative breast cancer.
Comprehensive full genome analysis of norovirus strains from eastern India, 2017–2021
Authors: Lo Mahadeb , Doan Yen Hai , Mitra Suvrotoa , Saha Ritubrita , Miyoshi Shin-ichi , Kitahara Kei , Dutta Shanta , Oka Tomoichiro , Chawla-Sarkar Mamta 
Published: January 2024

Abstract
Background
Worldwide, noroviruses are the leading cause of acute gastroenteritis (AGE) in people of all age groups. In India, norovirus rates between 1.4 to 44.4% have been reported. Only a very few complete norovirus genome sequences from India have been reported.

Objective
To perform full genome sequencing of noroviruses circulating in India during 2017–2021, identify circulating genotypes, assess evolution including detection of recombination events.

Methodology
Forty-five archived norovirus-positive samples collected between October 2017 to July 2021 from patients with AGE from two hospitals in Kolkata, India were processed for full genome sequencing. Phylogenetic analysis, recombination breakpoint analysis and comprehensive mutation analysis were also performed.

Results
Full genome analysis of norovirus sequences revealed that strains belonging to genogroup (G)I were genotyped as GI.3[P13]. Among the different norovirus capsid-polymerase combinations, GII.3[P16], GII.4 Sydney[P16], GII.4 Sydney[P31], GII.13[P16], GII.16[P16] and GII.17 were identified. Phylogenetic analysis confirmed phylogenetic relatedness with previously reported norovirus strains and all viruses were analyzed by Simplot. GII[P16] viruses with multiple residue mutations within the non-structural region were detected among circulating GII.4 and GII.3 strains. Comprehensive mutation analysis and selection pressure analysis of GII[P16] viruses showed positive as well as negative selection sites. A GII.17 strain (NICED-BCH-11889) had an untypeable polymerase type, closely related to GII[P38].

Conclusion
This study highlights the circulation of diverse norovirus strains in eastern India. These findings are important for understanding norovirus epidemiology in India and may have implications for future vaccine development.
Physical performance determinants in competitive youth swimmers: a systematic review
Authors: Price Todd , Cimadoro Giuseppe , S Legg Hayley 
Published: January 2024

AbstractBackgroundYouth swimming performance is determined by several physiological, biomechanical and anthropometric characteristics. This review aimed to identify physical performance determinants of youth swimming performance, assessing strength, power, anaerobic, aerobic and body composition measures. ̇MethodsSearches were conducted in electronic databases (PubMed and Web of Science) using keywords relating to swimming and physiological measures, supplemented by citation searching of similar reviews. A total of 843 studies were identified in the initial search. The following inclusion criteria were used: participants were competitive/trained swimmers; swimming time-trial or event was conducted; data was provided on one or more physiological parameters; study was published in English and peer-reviewed. A total of 43 studies met the inclusion criteria. Risk of bias was assessed using Joanna Briggs Institute (JBI) checklist.ResultsCross-sectional studies scored between 4–8 and randomised-controlled trials scored 8–9 on their respective JBI checklists. Youth swimming performance was determined by muscle strength, muscle power, lean body mass, anaerobic and aerobic metabolism measures in most studies, where improved performance values of these variables were conducive to swimming performance. Body fat percentage did not have a clear relationship in youth swimming performance.ConclusionsFindings of this review suggest that greater levels of muscle strength, muscle power and lean body mass are favourable in swimming performance, with muscle strength and muscle power particularly beneficial for start and turn performance. Anaerobic and aerobic metabolism measures were good determinants of swimming performance, with middle- and long-distance events more influenced by the latter. Body fat percentage has a nuanced relationship with swimming performance, where further investigation is required. Findings were inconsistent across studies, potentially due to unidentified confounding factors.Key points• Greater muscular strength and power qualities, anaerobic and aerobic capacities, and lean body mass are conducive to swimming performance.• Body fat percentage has a nuanced relationship with swimming performance.• Practitioners should consider general strength and power training as a useful tool to enhance performance in their youth competitors.
Relative risk of childhood and adolescence cancer in Iran: spatiotemporal analysis from 1999 to 2016
Authors: Hashemi Hasti , Mahaki Behzad , Farnoosh Rahman 
Published: January 2024

Abstract
Objective
Cancer is the third leading cause of death in the world with increasing trends in Iran. The study of epidemiology, trend, and geospatial distribution of pediatric cancers provides important information for screening as well as early detection of cancer and policy making. We aimed to assess the spatio-temporal disparity of childhood and adolescence cancer risk among provinces of Iran.

Methods
In this retrospective study, we estimated geospatial relative risk (RR) of childhood cancer in provinces of Iran using data from 29198 cases. We used BYM and its extended spatiotemporal model in Bayesian setting. This hierarchical model takes spatial and temporal effects into account in the incidence rate estimation simultaneously.

Results
The relative risk of cancer was > 1 for 45% of the provinces, where 27% of provinces had significantly ascending trend. North Khorasan, Yazd and Qazvin provinces had the highest risk rates while Sistan-Baluchistan province showed the lowest risk of cancer. However, the differential trends was highest in Sistan-Baluchistan, Bushehr, Hormozgan, and Kohgilouyeh-Boyerahmad. Both the point estimate and the trend of risk was high in Tehran.

Conclusion
The geographic pattern and trend of cancer in children seems to be different from that in adults that urges further studies. This could lead to increased health system capacity and facilitate the access to effective detection, research, care and treatment of childhood cancer.
Pediatric femoral shaft fracture requiring revision surgery for nonunion associated with vitamin D and K deficiencies: a case report
Authors: Igarashi Shun , Nozaka Koji , Shirahata Tsuyoshi , Kijima Hiroaki , Saito Hidetomo , Saito Kimio , Kawano Tetsuya , Miyakoshi Naohisa 
Published: January 2024

Abstract
Background
Nonunion of femoral shaft fractures in children is rare, and there is no clear treatment protocol. In this case report, a pediatric femoral shaft fracture that developed in nonunion due to vitamin deficiency after osteosynthesis, which was successfully treated with vitamin augmentation and replacement with a rigid antegrade intramedullary nail, is described.

Case presentation
The patient is an 11-year-old Japanese girl. She injured her right femoral shaft fracture when she hit a wall after kickboarding down a hill and underwent osteosynthesis with a titanium elastic nail. Six months postoperatively, she developed nonunion, was found to be deficient in vitamins D and K, and was started on vitamin supplementation. She underwent replacement with a rigid antegrade intramedullary nail at 7 months postoperatively, and bone union was achieved 3 months after reoperation.

Conclusion
When delayed union of a fracture is observed postoperatively, even in children without underlying disease, the cause of the problem must be investigated and treated promptly.
IDSL_MINT: a deep learning framework to predict molecular fingerprints from mass spectra
Authors: Baygi Sadjad Fakouri , Barupal Dinesh Kumar 
Published: January 2024

Abstract
The majority of tandem mass spectrometry (MS/MS) spectra in untargeted metabolomics and exposomics studies lack any annotation. Our deep learning framework, Integrated Data Science Laboratory for Metabolomics and Exposomics—Mass INTerpreter (IDSL_MINT) can translate MS/MS spectra into molecular fingerprint descriptors. IDSL_MINT allows users to leverage the power of the transformer model for mass spectrometry data, similar to the large language models. Models are trained on user-provided reference MS/MS libraries via any customizable molecular fingerprint descriptors. IDSL_MINT was benchmarked using the LipidMaps database and improved the annotation rate of a test study for MS/MS spectra that were not originally annotated using existing mass spectral libraries. IDSL_MINT may improve the overall annotation rates in untargeted metabolomics and exposomics studies. The IDSL_MINT framework and tutorials are available in the GitHub repository at https://github.com/idslme/IDSL_MINT.

Scientific contribution
Structural annotation of MS/MS spectra from untargeted metabolomics and exposomics datasets is a major bottleneck in gaining new biological insights. Machine learning models to convert spectra into molecular fingerprints can help in the annotation process. Here, we present IDSL_MINT, a new, easy-to-use and customizable deep-learning framework to train and utilize new models to predict molecular fingerprints from spectra for the compound annotation workflows.
Geographic and sociodemographic access to systemic anticancer therapies for secondary breast cancer: a systematic review
Authors: Pearson Sally Anne , Taylor Sally , Marsden Antonia , O’Reilly Jessica Dalton , Krishan Ashma , Howell Sacha , Yorke Janelle 
Published: January 2024

Abstract
Background
The review aimed to investigate geographic and sociodemographic factors associated with receipt of systemic anticancer therapies (SACT) for women with secondary (metastatic) breast cancer (SBC).

Methods
Included studies reported geographic and sociodemographic factors associated with receipt of treatment with SACT for women > 18 years with an SBC diagnosis. Information sources searched were Ovid CINAHL, Ovid MEDLINE, Ovid Embase and Ovid PsychINFO. Assessment of methodological quality was undertaken using the Joanna Briggs Institute method. Findings were synthesised using a narrative synthesis approach.

Results
Nineteen studies published between 2009 and 2023 were included in the review. Overall methodological quality was assessed as low to moderate. Outcomes were reported for treatment receipt and time to treatment. Overall treatment receipt ranged from 4% for immunotherapy treatment in one study to 83% for systemic anticancer therapies (unspecified). Time to treatment ranged from median 54 days to 95 days with 81% of patients who received treatment < 60 days.
Younger women, women of White origin, and those women with a higher socioeconomic status had an increased likelihood of timely treatment receipt. Treatment receipt varied by geographical region, and place of care was associated with variation in timely receipt of treatment with women treated at teaching, research and private institutions being more likely to receive treatment in a timely manner.

Conclusions
Treatment receipt varied depending upon type of SACT. A number of factors were associated with treatment receipt. Barriers included older age, non-White race, lower socioeconomic status, significant comorbidities, hospital setting and geographical location. Findings should however be interpreted with caution given the limitations in overall methodological quality of included studies and significant heterogeneity in measures of exposure and outcome. Generalisability was limited due to included study populations.
Findings have practical implications for the development and piloting of targeted interventions to address specific barriers in a socioculturally sensitive manner. Addressing geographical variation and place of care may require intervention at a commissioning policy level. Further qualitative research is required to understand the experience and of women and clinicians.

Systematic review registration
PROSPERO CRD42020196490
Basic counseling skills in psychology and teaching: validation of a short version of the counselor activity self-efficacy scales
Authors: Hunsmann Joanna Joy , Ay-Bryson Destina Sevde , Kobs Scarlett , Behrend Nicole , Weck Florian , Knigge Michel , Kühne Franziska 
Published: January 2024

Abstract
Background
Counseling self-efficacy is a relevant measure to examine trainees’ beliefs about their counseling skills. This study aimed to validate three scales of the revised German version of the Counselor Activity Self-Efficacy Scales (CASES-R) measuring basic counseling skills. To ascertain the scales’ sensitivity to change, counseling self-efficacy was assessed before and after specific training.

Method
The sample comprised 163 university students enrolled either in psychology or education. Students were examined before and after participating in training focusing on basic counseling skills. We applied confirmatory factor analysis and tested internal consistency, convergent validity, and criterion validity.

Results
Confirmatory factor analysis supported the three-factor structure of the CASES-R scales for basic counseling skills. The scales provided acceptable to good internal consistency (α = 0.77 − 0.87). Significant relations with general self-efficacy (r =.23, p <.01) provided first indication for convergent validity. We also found a significant correlation of the CASES-R with positive affect (r =.22), and significant correlations of some subscales with empathetic concern (r =.16 −.21) and mastery goal orientation (r =.16), overall supporting criterion validity. The CASES-R scales proved to be sensitive to change, as participants’ scores were higher after (M = 6.18, SD = 1.05) than before (M = 5.37, SD = 1.16) counseling training (F(1, 309) = 42.27, p <.001).

Conclusion
We found support for the proposed factor structure and reliability of the German version of the three CASES-R scales, indicating its suitability for use in basic counseling settings. Future research should further examine the scales’ validity.
Assessment of patients’ dental anxiety levels in the context of infectious diseases: development and validation of Musa Kazim’s Dental Anxiety Scale (MK-DAS)
Authors: Ucuncu Musa Kazim , Ucuncu Merve Yildirim 
Published: January 2024

AbstractObjectivesThe study aimed to develop and validate a new scale called Musa Kazim’s Dental Anxiety Scale (MK-DAS) to measure dental anxiety in relation to infectious diseases.MethodsThe study utilized a cross-sectional design and recruited participants from Faculty of Dentistry, Altinbas University. The sample included 289 participants who were seeking dental treatment. The Modified Dental Anxiety Scale (MDAS) was employed for the purpose of assessing levels of dental anxiety. In contrast, the MK-DAS, comprised a series of seven inquiries specifically targeting concerns regarding the treatment procedure and the fear of contagion. The data was analyzed using various statistical methods, including descriptive statistics, exploratory factor analysis, criterion validity, cluster analysis for cut-off points, and test-retest reliability.ResultsThe factor analysis revealed that MK-DAS had a two-factor structure. The first factor consisted of five items related to various aspects of the treatment process (α:0.837), while the second factor included two items related to the fear of infectious diseases (α:0.747). The scale showed good reliability, as indicated by high Cronbach’s alpha coefficients for both factors. Strong positive correlations were found between MDAS and the first factor of MK-DAS (r = 0.857;p < 0.01), moderate positive correlations between MDAS and the second factor (r = 0.323;p < 0.01), and a strong positive correlation between MDAS and the overall of MK-DAS (r = 0.782;p < 0.01). Additionally, the cluster analysis yielded a cut-off score of 17 based on the k-means analysis. Moreover, test-retest reliability analyses indicated that dimension 1 (ICC: 0.904), dimension 2 (ICC: 0.840), and overall MK-DAS (ICC: 0.944) demonstrated high internal consistency.ConclusionThe MK-DAS is an innovative and modern dental anxiety scale that has been proven to be reliable and valid, surpassing the comprehensiveness of the MDAS.
Zinc white marker paint in Mondrian’s neoplastic paintings
Authors: van der Werf Inez Dorothé , van den Berg Klaas Jan , Geldof Muriel , de Groot Suzan , Gross Markus , Hoppe Ruth , Hürlimann Cathja , Kolkena Laura , Steckling Friederike 
Published: January 2024

Abstract
This research provides new insights into the composition of zinc white paints used by Piet Mondrian during his neoplastic period. Nine paintings, dated between 1921 and 1935, were studied, with a focus on three works in the collection of the Fondation Beyeler (Basel, Switzerland)—Tableau I (1921–1925), Composition with yellow and blue (1932) and Composition with double line and blue (1935)—and on Lozenge composition with yellow lines (1933) in Kunstmuseum Den Haag (The Netherlands). Cross sections from other paintings, most of which previously studied by Van Asperen de Boer in the early 1990’s, were reexamined as well. The analyses revealed a zinc white paint with aluminum phosphate inclusions (ZW-Al/P). In two of the works, a zinc white paint with aluminum sulphate was also found. The occurrence of aluminum phosphate (or aluminum sulphate) in 1920–1930’s paint formulations, and in paintings by Mondrian, has never been reported so far. Likely, the use of this zinc white paint in Mondrian’s and other artworks is more widespread than currently known, but it may have been overlooked in similar case studies, since its identification can only be accomplished with detailed scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX) analysis of cross sections. Mondrian’s use of pure ‘bright’ ZW-Al/P paint is limited to the later paintings (1932–1935) of this study, whereas in the earlier works (1921–1929) it was mostly mixed with lead white, likely conveying a different hue. Both the ZW-Al/P and Zn-Al/S ‘marker paints’ were also added to yellow, blue or black paint in specific paint layers of the planes and lines, respectively. It was also shown that Mondrian used the same paint on the frames as in the white planes and that the frames were painted while still working on the composition, and not only when he considered the work completed. Indeed, the detection of the zinc white marker paints might help to delineate the chronology of Mondrian’s working process in other paintings and may also be important in the interpretation of overpaints in the course of a treatment when removal of certain paint layers is considered.