bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virome characterization of field-collected rodents in suburban Beijing reveals a spectrum of emerging pathogens
Authors: Hu Zhen-Yu , Tang Fang , Zhang Jing-Tao , Zhang Yun-Fa , Si Guang-Qian , Fu Bo-Kang , Wang Gang , Li Shuang , Zhang Lei , Peng Cong , Ma Xiao-Fang , Liu Yan-Tao , Bao Peng-Tao , Zhang Xiao-Ai , Liu Wei , Zhang Mei-Qi 
Published: February 2024

Abstract

Background
Rodents serve as natural reservoirs and transmission hosts for numerous zoonotic viruses, which can cause a range of animal and human diseases, posing significant public health concerns. Analyzing the viral diversity harbored by rodents is crucial for early warnings of emerging infectious diseases.
Results
By conducting meta-transcriptomic sequencing on spleen samples obtained from 432 wild rodents across three habitats, we characterized the high-depth RNA virome of wild rodents representing 9 species of two prominent mammalian families (Cricetidae and Muridae) in suburban Beijing. The composition of virome varied significantly at the virus family level among the nine rodent species and three types of natural habitats. A total of 142 viral species associated with vertebrates (n = 133) and invertebrates (n = 9) were identified from 26 families, including 75 novel viruses and 67 known viruses, thereby substantially expanding our knowledge about the diversity of rodent virome. Among these, twenty-five viruses were classified as high-risk, including 8 zoonotic viruses and 17 spillover-risk viruses. Additionally, nine previously unreported viruses were discovered for the first time in China. Furthermore, thirty-three viruses exhibited species transmission potential and some had evolutionary significance.
Conclusions
These findings enhance our understanding of rodent virome in Suburban Beijing and suggest that there is vast array of undiscovered viruses within these rodent species in China. Understanding the composition of rodent virome might provide insights into the potential risk of zoonotic spillover to humans.
suPAR is a potential biomarker of stage III-IV, grade C periodontitis through the impact of post-radiotherapy on head and neck cancer patients
Authors: Al-Kubaisi Ahmed A. , Ghazi Maysam Abdulrahman , Majeed Nisreen Salah , Aldelaimi Ekram R. , Enezei Hamid H. 
Published: February 2024

Abstract

Background: The urokinase-type plasminogen activator receptor (uPAR) plays an essential function in leukocytes and endothelial homeostasis and, therefore, in the development of chronic periodontitis.
Methods: The study enrolled 150 participants, including 50 (CP+HNC post-RT) patients, 50 (CP) without HNC patients, and 50 healthy controls. Clinical Attachment Loss (CAL), Probing Pocket Depth (PPD), Plaque Index (PI), and Gingival Bleeding Index (GBI) were recorded. An enzyme-linked immunosorbent assay (ELISA) was constructed to quantify serum (suPAR) levels.
Results: Stage and grade of periodontitis were stage III-IV, grade C in patients (CP+HNC post-RT), stage I-III, grade A/B in patients (CP without HNC), and absent in (healthy). Chronic periodontitis with HNC post-RT patients presented a significantly higher proportion of suPAR levels (506.7 pg/ml) compared to chronic periodontitis without HNC and healthy controls (423.08 pg/ml and 255.9 pg/ml), respectively. A significant positive correlation was found between serum suPAR levels and CAL, PPD, PI, and GBI in the periodontal disease groups. ROC results of suPAR (AUC=0.976 for CP+HNC post-RT, AUC=0.872 for CP without HNC). Hyposalivation was decreased in patients (CP+HNC post-RT; 0.15 [0.11-0.23] ml/min, P=0.001) and (CP without HNC; 0.30 [0.25-0.41] ml/min, P=0.001), compared to healthy controls; 0.35 [0.28-0.54] ml/min, P=0.001).
Conclusion: The study showed a significant elevation in serum suPAR levels in CP+HNC post-RT patients compared to the CP without HNC and control groups.
Polyvinyl chloride promoted the dissemination of antibiotic resistance genes in Chinese soil: A metagenomic viewpoint
Authors: Zhao Shuwen , Zhang Qianru , Huang Qilan , Zhang Chuchen 
Published: February 2024

Abstract
Microplastics (MPs) are becoming progressively widespread in the surrounding and are regarded as vectors of antibiotic resistance genes (ARGs). Soils of various properties carry large amounts of microplastics and ARGs. However, a thorough research evaluating the impact of different regions of China in evolving antibiotic resistome in soil MPs is insufficient. Here, we engaged a massive investigation by putting Polyvinyl chloride microplastics (PVC) into soil in 20 provinces of China which have different physical and chemical properties. The results showed that PVC could significantly affect soil bacterial community structure and ARGs abundance. Structural equation models showed that the addition of PVC changed the characteristics of the soil, which in turn influenced the bacterial community in the soil (which included ARG-containing bacterial hosts) and, ultimately, the relative abundance of ARGs. This work improves our comprehension of the effects of microplastics on the proliferation and hosts of ARGs in various soil environments, and it serves as a crucial reference for future plastic consumption and disposal.
The Effect of the Mechanical Densification Process in Wood Material on the Surface Adhesion Resistance of Varnishes
Authors: Atilgan Abdi , Burdurlu² Erol , Atar² Musa , Yasar³ Sekip Sadiye 
Published: February 2024

Abstract

This research was carried out to find out the impact the mechanical densifying process has on wood material on the varnish adhesion resistance to surface. To this end, the experiment samples acquired from black pine (Pinus nigra) and Uludag Fir (Abies bornmulleriana Mattf) were subjected to densification in a hydraulic press at 140°C and a rate of 25% and 50% with a direction of radial. Following the process of densification, the sanded and unsanded samples were varnished with cellulosic and polyurethane varnishes, afterwards the surface adhesion resistance of the varnishes was determined. Regarding the three-way interaction between densification ratio, surface treatment and wood type, the highest surface adhesion resistance of the varnish layer was found in black pine + unsanded surface + 25% densification (1.98), and the lowest was in Uludag Fir + unsanded surface + 50% densification (0.99). Concerning the triple interaction of varnish type, concentration ratio and surface treatment, the highest surface adhesion resistance of the varnish layer was found in the polyurethane varnish + non-densified control group + sanded surface (2.23), and the lowest was in cellulosic varnish + non-densified control group + sanded surface (1.02). Approximate values were obtained for the densification process. It can be stated that the densification process creates high adhesion values for the polyurethane varnish in the black pine wood type. The sanding process has an intensifier effect on these values, and the products which were obtained from the polyurethane varnished samples do not require sanding. Considering these situations can provide significant advantages in projects with wood materials subjected to the densification process.
Regional high iron deposition on brain quantitative susceptibility mapping correlates with cognitive decline in chronic kidney disease patients
Authors: Li Yuan , Jiang Yuhan , Gao Bingbing , Liu Na , Zhang Yukun , Zhou Huiling , Song Qingwei , Wang Nan , Miao Yanwei 
Published: February 2024

Abstract

Objective
Evaluate the changes of gray matter nuclei iron deposition in chronic kidney disease (CKD) patients by quantitative susceptibility mapping (QSM) threshold method and analyze the relationship between brain iron and cognitive function.
Materials and Methods
Prospectively recruited CKD patients with and without hemodialysis (HD and NHD), and healthy controls (HCs). Compared neuropsychological scores, clinical indexes and QSM data among three groups. Analyzed correlation between QSM data with neuropsychological scores and laboratory index in HD group. Used linear regression analysis to explore the effect of iron deposition on cognition and emotion in HD group. A statistically significant P-value was set at 0.05.
Results
96 individuals were recruited, including 35 HD patients (57.54 ± 10.42 years, 21 males), 18 NHD patients (55.06 ± 11.47 years, 10 males) and 43 HCs (55.67 ± 7.79 years, 18 males). Right RN mean-MSV increased in HD patients than HCs (P = 0.006). Left CN, bilateral Put and right RN high-MSV were significantly different among three groups (all P = 0.027, FDR-correction). Left Put high-MSV was positively correlated with Cre and UA, right Put high-MSV was negatively correlated with MCH and MCHC. High-MSV of some nuclei were negatively correlated with cognition. Regression analysis showed iron deposition in some nuclei were independent risk factors of depression and orientation ability.
Conclusion
Brain iron deposition is excessive and uneven in CKD patients, especially undergoing hemodialysis. Regional high-iron deposition can better assess the distribution of iron, which is related to the cognitive dysfunction and emotional disorder.
The prediction of estimated cerebral perfusion pressure with trans-systolic time in preterm and term infants
Authors: Zuiki Masashi , Ohta Mikito , Fujita Naoe , Uda Daisuke , Uesugi Madoka , Yamano Akio , Ichise Eisuke , Morimoto Hidechika , Hashiguchi Kanae , Kinoshita Daisuke , Hasegawa Tatsuji , Iehara Tomoko 
Published: February 2024

Abstract
Purpose
It is important to monitor cerebral perfusion in infants because hypo- and hyperperfusion can contribute to neurological injury. This study aimed to clarify the relationship between trans-systolic time (TST) and critical closing pressure (CrCP) or estimated cerebral perfusion pressure (CPPe) in neonates. Moreover, we aimed to determine the TST values in preterm and term infants with stable cerebral perfusion to clarify normative reference data.
Methods
This multicentre prospective study included infants with arterial lines admitted to the neonatal intensive care units between December 2021 and August 2023. TST, CrCP, and CPPe were calculated using middle cerebral artery waveforms recorded using transcranial Doppler ultrasonography when clinicians collected arterial blood samples.
Results
Three hundred and sixty samples were obtained from 112 infants with a gestational age of 32 (interquartile range, 27–37) weeks and a birth weight of 1481 (956–2355) g. TST was positively correlated with CPPe (r = 0.60, p < 0.001), but not with CrCP (r = 0.08, p = 0.10). The normative reference values of TST in preterm and term infants without samples of hyper- or hypocapnia and/or hyper- or hypotension, which may affect cerebral perfusion, were as follows: ≤ 29 weeks, 0.12 (0.11–0.14) s; 30–36 weeks, 0.14 (0.12–0.15) s; and ≥ 37 weeks, 0.16 (0.14–0.17) s, respectively.
Conclusions
TST in neonates significantly correlated with CPPe, but not with CrCP. This parameter may be a good predictor of cerebral perfusion and potentially have wider clinical applications.
Parents' Protective Strategies in Dealing with Adolescence Cancer: A Qualitative Study
Authors: Padehban Vahid , Yazdi Khadijeh . , poorkhani Amir Houshang , Mancheri Hamideh , sabzi Zahra 
Published: February 2024

Abstract
Background:
Being diagnosed with cancer is a very unpleasant and unbelievable experience for the family and the patient. The association of this disease with adolescence as the most dangerous developmental stage can be accompanied by an increasing line of psychological and social problems. The aforementioned research was conducted to explain the performance of parents in caring for Adolescence with cancer.
Methods:
The present qualitative study was conducted using the conventional content analysis method. 17 Iranian families with Adolescence diagnosed with cancer from December 2022 to November 2023 were selected as participants based on the inclusion criteria through targeted sampling. Continuous comparative data collection and analysis until reaching data saturation was done through semi-structured interviews. Data analysis was done using the qualitative content analysis method proposed by Granheim Velangman. The research report was presented using the Consolidated Criteria for Qualitative Research Reporting (COREQ) checklist.
Results:
The family's dominant strategy against Adolescence cancer includes 5 main concepts, included: "Parents' conscious approach appropriate to the adolescent when facing a cancer diagnosis," "Disease recognition based on multiple sources," "Using social support resources," "Family self-control methods when facing a cancer diagnosis," and "Limiting social interactions in the family."
Conclusions:
The findings showed that parents of Adolescence with cancer use protective strategies when facing their child's diagnosis and treatment process. Therefore, nurses can use these protective strategies in developing a care plan while considering the conditions of the parents and the affected adolescent to improve the health of the affected adolescent.
Combination Therapy with Anti-VEGF and Intravitreal Dexamethasone Implant for Treatment of Macular Edema Secondary to Retinal Vein Occlusion
Authors: Cai Xiaohui , Zhao Jing , Dang Yalong 
Published: February 2024

Abstract

Purpose
to compare the safety and efficacy of intravitreal injection of ranibizumab alone or ranibizumab combined with dexamethasone intravitreal implant in the treatment of macular edema secondary to retinal vein occlusion.
Study Design:
a single center, case-controlled, prospective cohort study (Clinical Trail Registration Number: ChiCTR2400080048).
Methods
patients with macular edema secondary to retinal vein occlusion who visited our hospital from August 2020 to August 2022 were enrolled. The patients were randomized into the ranibizumab group and the combination group (ranibizumab combined with dexamethasone intravitreal implant). The patients in both groups received ranibizumab intravitreal injections in the first three months as the initial treatment. However, in the ranibizumab group, patients might receive repeat injections during the follow-up in case of the recurrence of macular edema; in the combination group, patients received an intravitreal injection of dexamethasone implant after the first injection of ranibizumab at the day 15. The main outcome evaluations are best-corrected visual acuity and reduction of central macular thickness. The secondary outcome evaluations are the numbers of recurrence, the average injection interval, and the number of injections. Adverse events, such as ocular hypertension, and subconjunctival hemorrhage were also recorded.
Results
44 eyes were assigned to the ranibizumab group (n = 23) and the combination group (n = 21). Compared with the ranibizumab group, the combination group showed a significantly greater improvement in best-corrected visual acuity at 1, 3, 6, and 12 months postoperatively. Both groups showed a decrease in central macular thickness at 1 week and 1, 3, 6, and 12 months, compared with the baseline (P < 0.05). However, the combination group did not exhibit higher central macular thickness-reducing effects than the ranibizumab group (P > 0.05). Compared with the ranibizumab group, the combination group required a significantly lower average number of injections (P = 0.011) and had a significantly lower number of macular edema recurrences (P < 0.001) and significantly prolonged average time to re-injection (P = 0.05). The incidence of adverse events was not significantly different between the two groups (P = 0.944).
Conclusions
ranibizumab combined with dexamethasone intravitreal injection could sustainably and rapidly improve the best-corrected visual acuity of retinal vein occlusion patients with a good safety profile.
Fecal microbiota transplantation improves spatial learning disability caused by developmental anesthetic neurotoxicity in neonatal rats
Authors: Chaki Tomohiro , Horiguchi Yuri , Tachibana Shunsuke , Sato Satoshi , Hirahata Tomoki , Nishihara Noriaki , Kii Natsumi , Yoshikawa Yusuke , Hayamizu Kengo , Yamakage Michiaki 
Published: February 2024

Abstract
Background: Anesthetic exposure induces neurodegeneration in children. Although this problem has been elucidated in decades, the prophylaxis for developmental anesthetic neurotoxicity (DAN) has not been established. It has been reported that gut microbiota produces various metabolites and influences brain function of host, which has been called as Gut microbiota-Brain axis. We report the effect of fecal microbiota transplantation (FMT) on spatial learning disability caused by DAN in neonatal rats.
Methods: In experiment 1, neonatal rats were divided into C (Control) and S (Sevoflurane) groups to elucidate the effect of sevoflurane exposure on gut microbiota composition. In S group, rats were exposed by 2.1% sevoflurane for 2 hours in postnatal day (PND) 7-13. In experiment 2, neonatal rats were divided into S and SF groups. In SF group, neonatal rats were received FMT just after sevoflurane exposure in PND 7-13. The sample of FMT was obtained from non-anesthetized mother rat. Behavioral tests were performed to evaluate spatial learning ability from PND 26-39.
Results: Sevoflurane exposure significantly altered the gut microbiota composition. Especially, the relative abundance of Bacteroidetesphylum was significantly increased and that of Firmicutes phylum was significantly decreased by sevoflurane exposure. The FMT improved spatial learning ability. The microbiota analysis revealed that the α-diversity of gut microbiota was increased by FMT. Particularly, FMT decreased the relative abundances of Bacteroidetes phylum, Bacteroidia class, Bacteroidales order, Bacteroidaceae family, Bacteroides genus. Meanwhile, the relative abundances of Firmicutes phylum, Clostridia order, Clostridiales class, Ruminococcaceae family, Ruminococcus genus, and butyric acid-producing bacteria increased by FMT. Moreover, the FMT increased the fecal concentration of butyrate, and exerted the histone acetylation and the mRNA expression of brain derived neurotrophic factor in hippocampus. Immunofluorescence staining with Iba-1 antibody revealed that microglia infiltration in hippocampus was significantly suppressed by FMT. The mRNA expressions of apoptosis-inducing proteins were significantly suppressed and those of anti-apoptotic proteins were significantly promoted by FMT. The TUNEL staining indicated that neuronal apoptosis in hippocampus was significantly suppressed by FMT.
Conclusions: FMT improved spatial learning ability in rats with DAN. The modulation of gut microbiota might be an effective prophylaxis for DAN in children.
Efficacy of simple intravitreal gas injection for treating unclosed idiopathic macular holes after vitrectomy
Authors: Dou Zexia , Han Jindong , Zhao Shaozhen 
Published: February 2024

Abstract

Background
This study aimed to observe the efficacy of simple intravitreal injection of C3F8 in the treatment of unclosed idiopathic macular holes (IMH) in patients after primary pars plana vitrectomy (PPV).
Methods
This was a retrospective clinical observational study. Patients diagnosed with unclosed IMH who underwent primary PPV combined with internal limiting membrane peeling (ILMP) and air tamponade were included in the study. Optical coherence tomography (OCT) at 1 week after PPV revealed unclosed IMH with the "cuff" sign and intraretinal cysts. The next day, the patients were treated by simple intravitreal injection of C3F8. All patients underwent best corrected visual acuity (BCVA), indirect ophthalmoscopy, fundus photography, and OCT before PPV and at 1 week, before and after intravitreal gas injection for 1 to 3 months.
Results
The minimum horizontal hole diameter (MHD) before PPV was 555.4 ± 104.8 µm, and that at 1 week after PPV was 335.1 ± 74.3 um, with statistically significant (t = 11.256, P < 0.001). The percentage of closed holes after air tamponade was 100%. The mean BCVA before PPV was 0.79 ± 0.26 logMAR (20/123), and that after air tamponade was 0.48 ± 0.19 logMAR (20/30) with statistical difference significantly (t = 5.303, P = 0.002).
Conclusion
For patients with enclosed IMH after primary PPV, if OCT reveals the "cuff" sign, retreatment can be performed through simple intravitreal gas injection. The method is simple, economical, practical, and effective.
Rapid assessment of bio-distribution and antitumor activity of the photosensitizer Bremachlorin in a murine PDAC model: detection of PDT-induced tumor necrosis by IRDye® 800CW Carboxylate, using whole-body fluorescent imaging
Authors: Morrow Roisin Mc , de Bruijn Henriette S. , Que Ivo , Stuurman Debra C. , de Ridder Corrina M.A. , Doukas Michail , Robinson Dominic J. , Mezzanotte Laura , Lowik Clemens W.G.M. 
Published: February 2024

Abstract
Photodynamic therapy (PDT) is a light-based anticancer therapy that can induce tumor necrosis and/or apoptosis. Two important factors contributing to the efficacy of PDT are the concentration of the photosensitizer in the tumor tissue and its preferential accumulation in the tumor tissue compared to that in normal tissues. In this study, we investigated the use of optical imaging for monitoring whole-body bio-distribution of the fluorescent (660 nm) photosensitizer Bremachlorin in vivo, in a murine pancreatic ductal adenocarcinoma (PDAC) model. Moreover, we non-invasively, examined the induction of tumor necrosis after PDT treatment using near-infrared fluorescent imaging of the necrosis avid cyanine dye IRDye®-800CW Carboxylate. Using whole-body fluorescence imaging, we observed that Bremachlorin preferentially accumulated in pancreatic tumors. Furthermore, in a longitudinal study we showed that 3 hours after Bremachlorin administration, the fluorescent tumor signal reached its maximum. In addition, the tumor-to-background ratio at all-time points was approximately 1.4. Ex vivo, at 6 hours after Bremachlorin administration, the tumor-to-muscle or -normal pancreas ratio exhibited a greater difference than it did at 24 hours, suggesting that, in terms of efficacy, 6 hours after Bremachlorin administration was an effective time point for PDT treatment of PDAC. In vivo administration of the near infrared fluorescence agent IRDye®-800CW Carboxylate showed that PDT, 6 hours after administration of Bremachlorin, selectively induced necrosis in the tumor tissues, which was subsequently confirmed histologically. In conclusion, by using in vivo fluorescence imaging, we could non-invasively and longitudinally monitor, the whole-body distribution of Bremachlorin. Furthermore, we successfully used IRDye®-800CW Carboxylate, a near-infrared fluorescent necrosis avid agent, to image PDT-induced necrotic cell death as a measure of therapeutic efficacy. This study showed how fluorescence can be applied for optimizing, and assessing the efficacy of, PDT.