bluuebunny/crossref_metadata_2025_split huggingface dataset:
Auto-ICell: An Automated and Cost-Effective Integrated Droplet Microfluidic Platform for Real-Time Analysis of Single-Cell Morphology and Apoptosis
Authors: Wei Yuanyuan , Lin Meiai , Luo Shanhang , Abbasi Syed Muhammad Tariq , Tan Liwei , Cheng Guangyao , Bai Bijie , Ho Yi-Ping , Yuan Wu , Ho Ho-Pui 
Published: December 2023

Abstract
Existing single-cell analysis methods based on droplet microfluidics face challenges related to high chip costs and the lack of accurate, time-efficient, and high-throughput analysis techniques. To address these limitations, we present the Auto-ICell, a cost-effective droplet microfluidic system that integrates a 3D-printed microfluidic chip with automated image analysis algorithms. The Auto-ICell enables the generation of uniform droplet reactors and real-time analysis of single-cell morphology and apoptosis. With a throughput of 1,500 droplets per minute and droplet diameters ranging from 70 to 240 μm, the system employs colour-based and deep-learning-enabled algorithms for the analysis of droplet encapsulation and cell morphology, respectively. The Auto-ICell achieves an accuracy of over 91% and processes images in less than 0.03 seconds, eliminating the need for specialized facilities or trained operators. Its versatility extends to applications in cell culture, microreactors, drug carriers, assays, synthetic biology, and diagnostics. This study showcases the potential of the Auto-ICell system in advancing biological research and personalized disease treatment.
Progressive multifocal leukoencephalopathy associated with systemic lupus erythematosus: longitudinal observation of lymphocytes, JC virus in cerebrospinal fluid, and brain magnetic resonance imaging
Authors: Yamada Hidetada , Toko Megumi , Nakamori Masahiro , Ueno Hiroki , Aoki Shiro , Sugimoto Tomohiro , Yasutomi Hiroko , Nakamichi Kazuo , Maruyama Hirofumi 
Published: December 2023

Abstract
Progressive multifocal leukoencephalopathy (PML) rarely occurs in patients with systemic lupus erythematosus (SLE). This report presents a patient who developed PML due to SLE-associated lymphocytopenia. A 60-year-old woman diagnosed with SLE undergoing multiple immunosuppressive therapies, including azathioprine, presented with cerebral cortical symptoms, lymphocytopenia, and vitamin B12 deficiency and was subsequently diagnosed with SLE-associated PML. We evaluated the cause and disease activity of PML, focusing on the longitudinal assessment of lymphocytopenia, JC virus (JCV) DNA copy number in the cerebrospinal fluid, and magnetic resonance imaging (MRI) findings. Immunosuppressive therapy contributes to lymphocytopenia and PML; discontinuing azathioprine and initiating alternative immunosuppressive treatments (with intramuscular vitamin B12 injections) affected lymphocytopenia and disease management. The large hyperintense and punctate lesions observed on the fluid-attenuated inversion recovery images exhibited varying behaviors, indicating fluctuating PML disease activity. In addition, prominent hypointense signal rims were evident on susceptibility-weighted imaging during the late stages, particularly when JCV DNA copies were low. Clinicians should be meticulous when assessing the underlying pathology of lymphocytopenia owing to the multifactorial causes of PML due to SLE. This case’s time-dependent changes in lymphocytopenia, JCV DNA copy number, and MRI findings could provide clinicians with valuable assessment support.
Ocean Tides on Planets Orbiting Low-mass Stars
Authors: Yang Jun , Shi Jiaru , Abbot Dorian , LIU Yonggang , Kang Wanying , Lin Yufeng , Hu Yongyun 
Published: December 2023

Abstract
Planets in the liquid-water habitable zone of low-mass stars experience large tidal forces, 103 to 104 times those on Earth, due to the small distance between the habitable zone and the host star. Here, for the first time, we perform global ocean tide simulations and show that ocean tides on the planets with large eccentricities can reach 100-1000 m in height and ~10 m s-1 in flow speed. Interactions between tide and bottom topography can induce large energy dissipation, 10-100 W m-2 in global mean. This tidal energy dissipation can strongly accelerate orbital evolution by 1-2 orders, from ~1010 to 109 or 108 Earth years. Through global climate simulations, we further show that this level of tidal dissipation can strongly warm the surface, melt sea ice, and even push the system into a catastrophic runaway greenhouse state, within which the existence of life is impossible. For planets with small eccentricities, the ocean tides are much weaker.
Psychological impact of acne and post inflammatory hyperpigmentation among Sudanese women
Authors: Abdelgadir Hiba Salah , Abdelgadir Hind Salah , Ahmed Mosab Abdelgadir 
Published: December 2023

Abstract

Background:
Acne vulgaris is a chronic inflammatory disorder of the skin which often causes a negative impact on a woman's psychological state and quality of life. This study seeks to provide basic data and information about the psychological impact of acne on Sudanese women.
Materials and methods:
A cross-sectional questionnaire-based study was conducted during the period from June 2022 to January 2023. A total of 400 females aged 15 years and above were included in this study. These are Sudanese women who had suffered from acne in their lives, and who responded to the online study questionnaire. Cardiff Acne Disability Index (CADI) was used to assess the psychological effect of acne.
Results:
Seventy one percent of respondents had active acne. Acne scars were observed in (74.8%) of participants, while post-acne hyperpigmentation was observed in (71.3%). Facial acne was the most common, as it was observed in (75.8%) of the respondents. The mean age at acne onset was 15.66 years and ranged between 10 and 36 years. Mild and moderate acne were the most common types, accounting for (37.0%) and (41.5%) of cases, respectively. Acne was found to affect the psychological state of the vast majority of the respondents (89%). Cosmetics or makeup were used by (42.3%) of the respondents to conceal acne and scars. Late onset of acne treatment was reported in (42%) of the cases. Most respondents (72.5%) visited doctors to treat acne. The mean Cardiff Acne Disability Index (CADI) score was 4.55, which reflects mild effects of acne on the quality of life of most of the respondents.
The adverse psychological impact was found to be significantly associated with increased acne severity, scarring, and hyperpigmentation as well as with university educated respondents.
Conclusion:
It was found that acne has mild effects on the quality of life of Sudanese women. Late onset of acne treatment resulted in widespread scarring and post-inflammatory hyperpigmentation in most participants, which was associated with the psychological impact of acne. There is an urgent need to encourage early treatment of acne to reduce the physical sequalae of acne to reduce the psychosocial consequences associated with the disease and the risk of scarring and hyperpigmentation on Sudanese women. Sudanese health authorities and Sudanese doctors can address this issue through education and awareness programs.
Effect of pretreatment with a small dose of esketamine on sufentanil-induced cough during anesthesia induction: A randomized controlled trial
Authors: GAO Liangliang , Zhang Zhuoliang , Zhu Yi , Lu Xinyu , Tian Yue , Wei Lei 
Published: December 2023

Abstract
Background
Sufentanil-induced cough is common during the induction of anesthesia. The objective of this study was to determine whether pretreatment with a small dose of esketamine is effective in treating sufentanil-induced cough.
Methods
220 patients were screened, and 200 patients who had scheduled elective surgery and were between 18 and 70 years old were randomly divided into two groups. Before sufentanil was administered, group K was injected with 0.15 mg/kg esketamine at 5 s, and group C was administered with the same volume. Within 1 minute after sufentanil(0.4ug/kg) injection during induction, cough incidence and severity were evaluated. After sufentanil was injected, we recorded its hemodynamic changes and side effects.
Results
In the group K and group C, there was an incidence of cough of 5 and 34%, respectively. The K group had a significantly lower incidence and severity of cough compared to the C group immediately after sufentanil injection (P < 0.05). MAP and HR did not differ significantly between the two groups during three different times of general anesthesia induction (P > 0.05).
Conclusion
In our study, we found that sufentanil-induced cough was significantly reduced by pretreatment with 0.15mg/kg esketamine, but with no significant changes in the hemodynamic status.
Trial registration:
Chinese Clinical Trial Registry (ChiCTR2200063821, registered date: 17/09/2022), http://www.chictr.org.cn
Stable Isotopes as a Predictor for Organic or Conventional Classification of Berries and Vegetables
Authors: Zhu-Barker Xia 
Published: December 2023

Abstract

As organic agriculture spreads globally, promising enhanced food quality and soil health, the challenge lies in verifying the authenticity of organic practices, notably in developing countries with limited oversight. This study offers a solution by examining the elemental composition of carbon (C) and nitrogen (N), particularly their stable isotopes (δ13C and δ15N), in seven crops grown under organic or conventional methods across various countries. Our findings reveal that, while total C and N content showed no significant differences across production methods or regions, the N stable isotope (δ15N) levels were markedly distinct between organic and conventional crops. This difference allows us to propose a δ15N threshold to reliably distinguish between organic and conventional raspberries and strawberries, with a very low error rate. These thresholds, backed by an extensive dataset (n = 791), hold promise as effective tools for confirming organic authenticity, providing a new standard for future validation of organic farming practices.
Orbital-scale Dynamics of the Eastern Asian Summer Monsoon
Authors: Sinha Nitesh , Timmermann Axel , Lee Sun-Seon , Jo Kyoung-Nam , Wassenburg Jasper , Cleary Daniel , Yun Kyung-Sook 
Published: December 2023

Abstract
Due to multiple factors influencing orbital-scale variability of the East Asian Summer monsoon (EASM), the interpretation of speleothem-based climate reconstructions from this region has remained challenging. Using a 130,000-year accelerated astronomically-forced simulation conducted with the isotope-enabled Community Earth System Model, we determine the drivers of orbital-scale EASM variability in oxygen isotopes (δ18O). Indian summer monsoon (ISM) and associated moisture transport changes control the strong precipitation-δ18O variability in the western part of the EASM domain on timescales of 21,000 years. In the eastern part of the EASM region, including Korea and Japan, the interactions between ISM, western North Pacific Subtropical High, and westerlies control precipitation-δ18O. Thus, precessional variability in precipitation-δ18O is suppressed by the compensating effects of oceanic and continental moisture sources.
The influence of dual filial piety consciousness on mental health in Chinese youths is moderated by mobility and mediated by bidirectional intergenerational support
Authors: Zhou Jingjing , Zhao Qian , Zhang Huahua 
Published: December 2023

Abstract
Background: Previous studies have found the influence on mental health by filial piety (FP) among young people; however, there has been a lack of attention to the interactions between their dual FP (authoritarian vs. reciprocal) consciousness, bidirectional intergenerational support, and mental health. In the Chinese context, it is also unclear whether these interactions vary according to youth mobility (local vs. migrant). This study therefore explored the triple interactions between dual FP consciousness, bidirectional intergenerational support, and mental health in young people, and studied the mediating effect of bidirectional intergenerational support and the moderating effect of youth mobility therein.
Methods: Using the 2017 Chinese General Social Survey statistics, we defined young people as those aged 15-44 years. The final effective sample consisted of 1,400 individuals. Software SPSS v22.0 was used to organize the data and to conduct descriptive and correlation analyses, and SPSS PROCESS Macro v3.4.1 to test the mediating and moderating effects.
Results: With such variables as gender, age, ethnicity, education level, marital status, household registration type, personal annual income, and number of children controlled, we found that young people’s dual FP (authoritarian vs. reciprocal) consciousness and mental health were positively correlated (r = 0.281, p < 0.01; r = 0.406, p < 0.01, respectively). Higher dual FP consciousness corresponded to better mental health, and the influence of dual FP consciousness on mental health was mediated by bidirectional intergenerational support and moderated by mobility.
Conclusions: Our findings provide theoretical insights and practical pointers for improving the youth mental health. First, theoretical propaganda and practical activities should be combined to strengthen the publicity of the filial culture. Second, the role of family members should be given full play, because intergenerational mutual support helps maximize the interests of the family as a whole. Finally, young migrants should be guided vigorously to participate in community activities themed on “FP and respect for the elderly” to improve their FP consciousness. By forging harmonious intergenerational relationships, their mental health can be expected to improve.
WITHDRAWN: A Meta-Heuristic Based Approach for Optimal Fake News Detection Using Supervised Learning
Authors: Jaiswal Arunima , Verma Himika , Sachdeva Nitin , Wary Alongbar , Indra Gaurav , Brahma Daniel Kr. 
Published: December 2023

Abstract

The rapid dissemination of fake news in the digital age has raised concernsregarding the authenticity and credibility of online information. In this study, we propose an innovative approach for detecting fake news by combining the power of RoBERTa, a state-of-the-art language model, with the Firefly Optimization Algorithm (FOA) and Long Short-Term Memory (LSTM) neural networks. Our model leverages RoBERTa’s contextual understanding of text to extract informative features from news articles, including content, user engagement, and source characteristics. The FOA is employed to optimize the selection of these features, enhancing the model’s ability to discern between genuine and deceptive news. The selected features are then used as inputs to an LSTM network, which learns the temporal dependencies within the data for accurate classification. To validate the effectiveness of our approach, we conduct experiments using benchmark datasets such as ISOT and fakenewsnet specifically designed for fake news detection tasks. The experimental results demonstrate that our proposed model outperforms existing methods in terms of accuracy and other performance metrics. By integrating RoBERTa, FOA, and LSTM, our model effectively combines contextual understanding, feature selection optimization, and sequence modeling, enabling accurate identification of fake news articles. This research contributes to the advancement of fake news detection techniques and offers a promising solution to address the challenges associated with online misinformation, ultimately fostering trust and reliability in the digital information landscape.
Value of the Acute Physiology and Chronic Health Evaluation II (APACHE II) score in predicting the hospital mortality for postoperative brain tumor patients admitted to the intensive care units in Japan: A Retrospective Case-Control Study.
Authors: Azumi Mai , Mizobuchi Yoshifumi , Nakanishi Nobuto , Nakajima Kohei , Hara Keijiro , Fujihara Toshitaka , Ishihara Manabu , Oto Jun , Takagi Yasushi 
Published: December 2023

Abstract

Objective
No prognostic predictors for brain tumors based on ICU admission data have been reported. APACHE II is a score based on the data of patients admitted to the ICU and is often correlated with the severity of the disease and prognosis. However, no studies have reported an association between APACHE Ⅱand the prognosis of brain tumor patients. Since 2014, the Japanese Intensive care Patients Database (JIPAD) was established by the Japanese Society of Intensive Care Medicine (JSICM) to construct a high-quality ICU database as a national registry with the goal of improving the quality of care and pursuing the development of intensive care medicine in Japan. We used JIPAD to examine the factors associated with in-hospital mortality based on the background of postoperative patients with brain tumors admitted to the ICU and the data from the ICU.
Methods
Patients aged ≥16 years enrolled in JIPAD between April 2015 and March 2018 after surgery for brain tumors underwent craniotomy or biopsy. We examined factors related to outcomes at discharge from data such as blood tests and medical procedures during ICU admission, tumor type, and APACHE II score.
Results
Of the 1,454 cases (male:female ratio: 1:1.1, mean age: 62 years) included in the study, 32 cases (2.2%) died in hospital. In multivariate analysis, male (OR 2.70 [95%CI 1.21–6.03]), malignant tumor (OR 2.94 [95%CI 1.31–6.59]), ventilator use (OR 2.67 [95%CI 1.19–5.98]), APACH II score ≥ 15 (OR 5.46 [95%CI 2.49–11.9]) were significantly associated with in-hospital mortality.
Conclusion
Factors related to in-hospital mortality in postoperative brain tumor patients cannot be improved in advance, but by picking up cases with a high risk of in-hospital death at an early stage, it is possible to devise methods of treatment and support for the patient's family.
Integrated analysis of single-cell and bulk RNA-seq unravels the role of macrophage markers ceRNA networks in colorectal cancer’s immune microenvironment and chemotherapy sensitivity
Authors: Tian Jinhai , Jiang Zongying , Wang Jia , Zhang Shaoting , Cao Xu , Sun Jianmin 
Published: December 2023

Abstract
Background
Colorectal cancer (CRC) is a highly heterogeneous tumor with differences in gene expression and regulation among different cell types. Macrophages, which are highly heterogeneous cells, play important roles in anti-tumor responses, shaping the tumor immune microenvironment(TIME), and influencing tumor chemotherapy sensitivity. However, the precise roles of macrophages in these processes in CRC are not clear.
Methods
Gene expression data of CRC tissues (COADREAD) were downloaded from the TCGA database and underwent ID conversion, data cleaning, and normalization before analysis. Single-cell RNA sequencing (scRNA-seq) data were obtained from the GEO database. The gene expression profile of CRC tissues was analyzed using the R package, and scRNA-seq data were analyzed using the cell dex package. The limma package was used for the analysis of lncRNA, and online databases were used for the analysis of competitive endogenous RNA (ceRNA) network, TIME, and tumor response to chemotherapy.
Results
By t-SNE analysis on scRNA-seq data of CRC tissues, 17 clusters of cells were identified, which were further annotated as 9 cell types. Among these cell types, macrophages had the most interaction with other cells based on analysis of ligand-receptor gene pairs. By overall survival (OS) analysis, we found that the expression of 41 macrophage markers correlated with OS of CRC patients. The pattern of mRNA regulation by ceRNA is considered to play an important role in the regulation of gene expression in CRC. We analyzed the interaction of the identified 41 macrophage markers with lncRNA and miRNA and found that mRNAs of RPL28, CANX, VMO1, GNA13, ARL4C, TUBB, and RNASE1 had 22 interactions with 3 miRNAs and 9 lncRNAs. Furthermore, we found that the expression of these 7 molecules was highly correlated with multiple immune factors and immune cell infiltration levels in CRC tissues, as well as the sensitivity of patients to chemotherapeutic drugs such as Cisplatin, Camptothecin, Metformin, Paclitaxel, and Vinblastine. KEGG and GO enrichment analysis of these 7 molecules showed their involvement in key metabolic pathways related to the progression of CRC.
Conclusions
This study identified that the expression of RPL28, CANX, VMO1, GNA13, ARL4C, TUBB, and RNASE1 in macrophages is correlated with the overall survival of CRC patients. The ceRNA regulatory networks of these molecules are involved in the regulation of immune response and sensitivity of CRC to chemotherapy, as well as metabolic pathways in CRC.
Enhancing Physical Function and Activity Level in malnourished Older Adults through Oral Nutrition Supplements: A Randomized Controlled Trial
Authors: Weerasinghe Kalani , Jayawardena Ranil , Gamage Manoja , Hills Andrew P 
Published: December 2023

Abstract
Background: Malnutrition of older individuals, leads to significant functional decline, reducing their quality of life. Lifestyle interventions; dietary improvements and supplementation are explored to enhance the physical function of older adults. The current study aimed to assess the impact of oral nutritional supplements (ONS) on the functional and activity levels of the Sri Lankan elderly.
Methods: This included; an intervention group (IG) receiving 200 mL of ONS providing 247 kcal per serving, for 12 weeks and a control group (CG) receiving an equivalent volume of water. Changes in handgrip strength, knee extension strength, gait speed, functional and activity levels were assessed.
Results: The IG showed significant improvements in handgrip strength (43.96 ± 18.61 kg vs. 32.81 ± 17.92 kg; p < 0.001) and knee extension strength (23.45 ± 2.29 kg vs. 16.41 ± 2.09 kg; p < 0.001) following 12 weeks compared to the CG. The IG also exhibited significant improvements in gait speed (1.31 ± 0.52 m/s vs. 0.87 ± 0.26 m/s), Barthel index score, (0.30 ± 0.47 vs. -0.18 ± 0.66), PASE score (0.52 ± 17.79 vs. -1.60 ± 21.77) and IPAQ categories.
Conclusions: ONS was found to be effective in improving the functional and physical activity levels of malnourished older adults.
Trial registration: Sri Lanka Clinical Trial Registry SLCTR/2022/021. Registered on 06/10/2022.