bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collaborative effect of Csnk1a1 haploinsufficiency and mutant p53 in Myc induction can promote leukemic transformation
Authors: Fuchs Stijn N. R. , Stalmann Ursula S. A. , Snoeren Inge A. M. , Bindels Eric , Schmitz Stephani , Banjanin Bella , Hoogenboezem Remco M. , van Herk Stanley , Saad Mohamed , Walter Wencke , Haferlach Torsten , Seillier Lancelot , Saez-Rodriguez Julio , Dugourd Aurélien J. F. , Lehmann Kjong-Van , Ben-Neriah Yinon , Gleitz Hélène F. E. , Schneider Rebekka K. 
Published: December 2023

Abstract
It is still not fully understood how genetic haploinsufficiency in del(5q) myelodysplastic syndrome (MDS) contributes to malignant transformation of hematopoietic stem cells. We asked how compound haploinsufficiency for Csnk1a1 and Egr1 in the common deleted region on chromosome 5 affects hematopoietic stem cells. Additionally, Trp53 was disrupted as the most frequently comutated gene in del(5q) MDS using CRISPR/Cas9 editing in hematopoietic progenitors of wild-type (WT), Csnk1a1–/+, Egr1–/+, Csnk1a1/Egr1–/+ mice. A transplantable acute leukemia only developed in the Csnk1a1–/+Trp53–edited recipient. Isolated blasts were indefinitely cultured ex vivo and gave rise to leukemia after transplantation, providing a tool to study disease mechanisms or perform drug screenings. In a small-scale drug screening, the collaborative effect of Csnk1a1 haploinsufficiency and Trp53 sensitized blasts to the CSNK1 inhibitor A51 relative to WT or Csnk1a1 haploinsufficient cells. In vivo, A51 treatment significantly reduced blast counts in Csnk1a1 haploinsufficient/Trp53 acute leukemias and restored hematopoiesis in the bone marrow. Transcriptomics on blasts and their normal counterparts showed that the derived leukemia was driven by MAPK and Myc upregulation downstream of Csnk1a1 haploinsufficiency cooperating with a downregulated p53 axis. A collaborative effect of Csnk1a1 haploinsufficiency and p53 loss on MAPK and Myc upregulation was confirmed on the protein level. Downregulation of Myc protein expression correlated with efficient elimination of blasts in A51 treatment. The “Myc signature” closely resembled the transcriptional profile of patients with del(5q) MDS with TP53 mutation.
High-risk and silent clonal hematopoietic genotypes in patients with nonhematologic cancer
Authors: Stonestrom Aaron J. , Menghrajani Kamal N. , Devlin Sean M. , Franch-Expósito Sebastià , Ptashkin Ryan N. , Patel Swara Y. , Spitzer Barbara , Wu Xiaodi , Jee Justin , Sánchez Vela Pablo , Milbank Jennifer H. , Shah Ronak H. , Mohanty Abhinita S. , Brannon A. Rose , Xiao Wenbin , Berger Michael F. , Mantha Simon , Levine Ross L. 
Published: December 2023

Abstract
Clonal hematopoiesis (CH) identified by somatic gene variants with variant allele fraction (VAF) ≥ 2% is associated with an increased risk of hematologic malignancy. However, CH defined by a broader set of genotypes and lower VAFs is ubiquitous in older individuals. To improve our understanding of the relationship between CH genotype and risk of hematologic malignancy, we analyzed data from 42 714 patients who underwent blood sequencing as a normal comparator for nonhematologic tumor testing using a large cancer-related gene panel. We cataloged hematologic malignancies in this cohort using natural language processing and manual curation of medical records. We found that some CH genotypes including JAK2, RUNX1, and XPO1 variants were associated with high hematologic malignancy risk. Chronic disease was predicted better than acute disease suggesting the influence of length bias. To better understand the implications of hematopoietic clonality independent of mutational function, we evaluated a set of silent synonymous and noncoding mutations. We found that silent CH, particularly when multiple variants were present or VAF was high, was associated with increased risk of hematologic malignancy. We tracked expansion of CH mutations in 26 hematologic malignancies sequenced with the same platform. JAK2 and TP53 VAF consistently expanded at disease onset, whereas DNMT3A and silent CH VAFs mostly decreased. These data inform the clinical and biological interpretation of CH in the context of nonhematologic cancer.
Enhancing agricultural value chains through technology adoption: a case study in the horticultural sector of a developing country
Authors: Valdes Rodrigo , Gómez David , Barrantes Luz 
Published: December 2023

Abstract
Background
The development of agricultural value chains in developing countries has been the focus of much international interest, prompting the exploration of technology adoption strategies. These strategies hinge upon a multitude of factors like farmer characteristics, farm structure, location, organizational and institutional elements, as well as information-related factors.

Purpose
In this study, we delve into the influential social and productive factors that underpin technology adoption among small horticultural producers in Chile.

Design/methodology/approach
Data pertaining to the five primary horticultural crops in Chile—namely, corn, lettuce, tomato, cucumber, and onion—were collected, considering their respective cultivated areas. A comprehensive evaluation of 13 technologies encompassing cultural practices, crop improvement, and irrigation was conducted. The methodological approach comprised two stages. Initially, an ordered probit model was employed to analyze the adoption intensity of cultural practices, crop improvement, and irrigation. Subsequently, a Kruskal–Wallis test was utilized to compare the means across technology adoption groups.

Results
The findings reveal a positive correlation between technology adoption intensity and the level of education, composition of the family nucleus, and investment intensity. Moreover, the production system’s location emerged as the most critical determinant for technology adoption.

Conclusions
The factors under scrutiny furnish direct and indirect evidence of their impact on the productivity and competitiveness of agricultural value chains. Thus, this paper significantly contributes to comprehending the role of technology adoption in designing and executing rural development strategies within developing countries.
Analysis of the Genetic Characteristics and Metastatic Pathways of G1 and G2 Colorectal Neuroendocrine Neoplasms
Authors: Wang Zhijie , Chen Qichen , Zhao Fuqiang , Sun Li , Qiu Yixian , Cheng Huanqing , Qin Jiayue , Wang Huina , Shi Susheng , Cao Shanbo , Liu Qian 
Published: December 2023

Abstract

Objective
G1 and G2 colorectal neuroendocrine neoplasms (NENs) are a group of rare and indolent diseases. We aimed to delineate their genetic characteristics and explore their metastatic mechanisms.

Methods
We used next-generation sequencing technology for targeted sequencing for 54 patients with G1 and G2 colorectal NENs. We delineated their genetic features and compared the genetic characteristics between metastatic NENs and nonmetastatic NENs. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was utilized to explore their abnormal pathways and study their potential metastatic mechanisms.

Results
We collected 23 metastatic NENs and 31 nonmetastatic NENs. In the whole cohort, the common mutated genes were NCOR2, BRD4, MDC1, ARID1A, AXIN2, etc. The common copy number variations (CNVs) included amplification of HIST1H3D, amplification of HIST1H3E, and loss of PTEN. The KEGG enrichment analysis revealed that PI3K-Akt, MAPK, and Rap1 were the major abnormal pathways. There were significantly different genetic features between metastatic NENs and nonmetastatic NENs. The metastatic NENs shared only 47 (22.5%) mutated genes and 6 (13.3%) CNVs with nonmetastatic NENs. NCOR2, BRD4, CDKN1B, CYP3A5, and EIF1AX were the commonly mutated genes in metastatic NENs, while NCOR2, MDC1, AXIN2, PIK3C2G, and PTPRT were the commonly mutated genes in nonmetastatic NENs. Metastatic NENs presented a significantly higher proportion of abnormal pathways of cell senescence (56.5% vs 25.8%, P = .022) and lysine degradation (43.5% vs 16.1%, P = .027) than nonmetastatic NENs.

Conclusion
G1 and G2 colorectal NENs are a group of heterogeneous diseases that might obtain an increased invasive ability through aberrant cell senescence and lysine degradation pathways.
Reading Border through Desire: Queer Indigenous Theory, Nordic Settler Colonialism, and Trans Aesthetics
Authors: Kyrölä Kata 
Published: December 2023

Abstract
This article examines the Swedish fantasy-horror-romance film Gräns (Border, dir. Ali Abbasi, 2018) through queer Indigenous thought and the notion of trans aesthetics, exploring how the film may sensitize its viewers to seeing and feeling with gender variance, queer desire, and the trauma of settler colonialism. Drawing on Eve Tuck's call for desire-based research, the article asks what is at stake in queer, trans, and decolonial readings of films that are not necessarily identifiable as such at the surface level. Border centers on a love story between two gender-fluid trolls who pass as human and whose kin have been subjected to genocide, dislocation, and mutilation, but the film's reception largely misses the connection to the treatment of Indigenous Sámi people and transgender people within Nordic settler states. The article argues that Border's ecstatic depiction of gender-fluid desires, bodies, and sex, alongside its examination of the psychic consequences of settler colonial violence, make it a thus far unique film in the Nordic context — even though this examination happens through the distancing effect of trolls as metaphorical Natives. The main characters embody wrongness in the settler nation-state, in heteronormative society, and ultimately in the delimiting category of the human, but the film imagines rightness in nature as a queer, gender-fluid space where all creatures can just be. Through employing notions of trans aesthetics, the (non)sovereign erotic, refusal, and haunting, the article proposes desire-based readings of cinema that envision ways of feeling and existing beyond humancentric, settler, binary notions of gender and sexuality.
Their Bodies, Their Selves: Reflexivity and Embodiment in 2010s Women's Autobiographical Author-Performer TV Dramedies
Authors: Levy Yael 
Published: December 2023

Abstract
During the 2010s, American television witnessed a growing attention to and presence of women, both in TV representation and in TV creation and production. As part of that development, the self-representation of women who are both the creators and the stars of their shows — such as Lena Dunham (creator and star of Girls; HBO, 2012 – 17), Tig Notaro (creator and star of One Mississippi, Amazon, 2015 – 17), Issa Rae (creator and star of Insecure, HBO, 2016 – 21), Pamela Adlon (creator and star of Better Things, FX, 2016 – 22), and Frankie Shaw (creator and star of Smilf, Showtime, 2017 – 19) — has been on the rise. This article examines these five dramedies in which women both exert authorial voices in telling their own stories and bring to bear performative embodiment in starring in these autobiographical texts. The textual multilayeredness of these author-performers’ autobiographical dramedies, the article shows, reveals the ways in which authorship and performance operate in the realm of women's televisual representation.
Cost-Effectiveness Analysis of Hepatocellular Carcinoma Surveillance in Nonalcoholic Fatty Liver Disease Cirrhosis Using US Visualization Score C–Triggered Abbreviated MRI
Authors: Mulgaonkar Ashwini , Huang Daniel Q. , Siddiqi Harris , Fowler Kathryn , Sirlin Claude B. , Marks Robert , Loomba Rohit , Konijeti Gauree G. 
Published: December 2023

INTRODUCTION:Ultrasound (US) is associated with severe visualization limitations (US Liver Imaging Reporting and Data System visualization score C) in one-third of patients with nonalcoholic fatty liver disease (NAFLD) cirrhosis undergoing hepatocellular carcinoma (HCC) screening. Data suggest abbreviated MRI (aMRI) may improve HCC screening efficacy. This study analyzed the cost-effectiveness of HCC screening strategies, including an US visualization score–based approach with aMRI, in patients with NAFLD cirrhosis.METHODS:We constructed a Markov model simulating adults with compensated NAFLD cirrhosis in the United States undergoing HCC screening, comparing strategies of US plus visualization score, US alone, or no surveillance. We modeled aMRI in patients with visualization score C and negative US, while patients with scores A/B did US alone. We performed a sensitivity analysis comparing US plus visualization score with US plus alpha fetoprotein or no surveillance. The primary outcome was the incremental cost-effectiveness ratio (ICER), with a willingness-to-pay threshold of $100,000 per quality-adjusted life-year. Sensitivity analyses were performed for all variables.RESULTS:US plus visualization score was the most cost-effective strategy, with an ICER of $59,005 relative to no surveillance. The ICER for US alone to US plus visualization score was $822,500. On sensitivity analysis, screening using US plus visualization score remained preferred across several parameters. Even with alpha fetoprotein added to US, the US plus visualization score strategy remained cost-effective, with an ICER of $62,799 compared with no surveillance.DISCUSSION:HCC surveillance using US visualization score–based approach, using aMRI for visualization score C, seems to be the most cost-effective strategy in patients with NAFLD cirrhosis.
Extraintestinal Manifestations and Family History of Inflammatory Bowel Disease Increase the Risk of Pouchitis in a State-Level Epidemiology Study
Authors: Barnes Edward L. , Dunn Michael S. , Ashburn Jean , Barto Amy , Bloomfeld Richard , Cairns Ashley , Mehta Kurren , Patel Pooja , Dziwis Jennifer , Esckilsen Scott , Rahbar Reza , Sadiq Timothy S. , Hanson John S. , Herfarth Hans H. 
Published: December 2023

INTRODUCTION:
Our understanding of the epidemiology of inflammatory conditions of the pouch and effectiveness of treatment is largely based on selected populations. We created a state-level registry to evaluate the incidence of pouchitis and the effectiveness of treatments used in an initial episode of pouchitis.

METHODS:
In a state-level retrospective cohort of all patients undergoing proctocolectomy with ileal pouch-anal anastomosis (IPAA) for ulcerative colitis between January 1, 2018, and December 31, 2020, we evaluated the incidence of pouchitis and compared the proportion of patients developing recurrent pouchitis and chronic antibiotic-dependent pouchitis according to initial antibiotic therapy.

RESULTS:
A total of 177 patients underwent surgery with 49 (28%) developing pouchitis within the 12 months after the final stage of IPAA. Patients with extraintestinal manifestations of inflammatory bowel disease (IBD) were significantly more likely to develop pouchitis within the first 12 months after IPAA (adjusted odds ratio 2.45, 95% confidence interval 1.03–5.81) after adjusting for family history of IBD (adjusted odds ratio 3.50, 95% 1.50–8.18). When comparing the proportion of patients who developed recurrent pouchitis or chronic antibiotic-dependent pouchitis with those who experienced an isolated episode of pouchitis, there were no significant differences among the initial antibiotic regimens used.

DISCUSSION:
In a state-level examination of outcomes after IPAA for ulcerative colitis, patients with extraintestinal manifestations of IBD were more likely to develop pouchitis; however, the initial antibiotic regimen chosen did not seem to affect long-term outcomes.
Digital Cloning of the Dead: Exploring the Optimal Default Rule
Authors: Iwasaki Masaki 
Published: December 2023

Abstract
We conducted a survey experiment in the U.S. to analyze how the consent or dissent of a deceased individual influences the social acceptability of digital resurrection. The results showed a substantial relative treatment effect of consent versus dissent, with a 2-point difference in acceptability on a 5-point scale. When the deceased had consented, 58 % of respondents viewed digital resurrection as socially acceptable, whereas this number was only 3 % when the deceased had dissented. These findings suggest that relevant legal regulations should respect the decision of the deceased. Our study then explored the optimal default rule using observational research: 59 % of respondents were against the idea of their own digital resurrection. An opt-in rule seems socially desirable, where the default is the prohibition of digital resurrection, and exceptions allow it only with consent from the deceased.
Cellulose acetate oleate-reinforced poly(butylene adipate-co-terephthalate) composite materials
Authors: Chen Yi , Feng Ting , Long Yifei , Pan Cheng , Fan Guozhi , Juan Bai , Song Guangsen 
Published: December 2023

Abstract
Cellulose acetate oleate (CAO)-reinforced poly(butylene adipate-co-terephthalate) (PBAT) composites were prepared by the solvent casting method. The influence of the addition of CAO on the mechanical property, thermal property, disintegration property, compatibility, and hydrophobicity of PBAT/CAO composites was investigated. Compared with PBAT, the tensile strength and Young’s modulus of PBAT/CAO with 4 wt% CAO were increased by 9.5% and 25.7%, and the disintegration rate was also increased by 2.8 times. The results of morphological property, contact angle, and water vapor transmission indicated that the PBAT/CAO composites had good interfacial interaction and compatibility, and the hydrophobicity was improved. PBAT/CAO was applied to strawberry preservation, and it showed excellent freshness retention performance. Moreover, a possible degradation pathway for PBAT/CAO composite was proposed. This work provided a way for the preparation and performance improvement of biodegradable materials, which is expected to be applied in the packaging field.
Sustainable conversion of carbon dioxide into novel 5-aryldiazenyl-1,2,4-triazol-3-ones using Fe3O4@SP-vanillin-TGA nanocomposite
Authors: Nikpassand Mohammad , Fekri Leila Zare , Varma Rajender S. 
Published: December 2023

Abstract
A series of novel 5-aryldiazenyl-1,2,4-triazol-3-ones are synthesized at room temperature in short reaction time, and excellent yields via four-component reaction between 4-aryldiazenyl-salicylaldehydes, ammonium acetate, arylhydrazine, and carbon dioxide using bis(thioglycolic acid)-vanillin (2,2′-(((4-hydroxy-3-methoxyphenyl)methylene)bis(sulfanediyl))diacetic acid)‐functionalized silica-coated Fe3O4 magnetic nanocomposite (Fe3O4@SP-vanillin-TGA MNCs). The present protocol offers several advantages such as gentle reaction conditions, excellent performance, simple fabrication, separation methods, and reduction of detrimental environmental consequences. The catalyst could be easily recovered and reused for six runs with nearly steady activity. The structures of the synthesized 5-aryldiazenyl-1,2,4-triazol-3-ones have been confirmed with the aid of using 1H, 13C NMR, and fourier transform infrared spectral data and elemental analyses.
Determinants of prematurity in urban Indonesia: a meta-analysis
Authors: Marsubrin Putri Maharani Tristanita , Ibrahim Naufal Arkan Abiyyu , Dilmy Mohammad Adya Firmansha , Ariani Yulia , Wiweko Budi , Irwinda Rima , Harzif Achmad Kemal , Hegar Badriul , Basrowi Ray Wagiu 
Published: December 2023

Abstract

Objectives
Indonesia is the fifth country with the highest number of preterm births worldwide. More than a third of neonatal deaths in Indonesia were attributed to preterm birth. Residential areas affected the occurrence of preterm birth due to differing socioeconomic and environmental conditions. Many studies have investigated the determinants of prematurity in Indonesia, however, most of them were performed in rural areas. This study is the first meta-analysis describing the determinants of preterm birth in urban Indonesia, which aimed to become the foundation upon implementing the most suitable preventative measure and policy to reduce the rate of preterm birth.

Methods
We collected all published papers investigating the determinants of preterm birth in urban Indonesia from PubMed MEDLINE and EMBASE, using keywords developed from the following key concepts: “preterm birth”, “determinants”, “risk factors”, “Indonesia” and the risk factors, such as “high-risk pregnancy”, “anemia”, “pre-eclampsia”, and “infections”. Exclusion criteria were multicenter studies that did not perform a specific analysis on the Indonesian population or did not separate urban and rural populations in their analysis, and articles not available in English or Indonesian. The Newcastle Ottawa Scale was used to assess the risk of bias. This systematic review was registered in PROSPERO.

Results
Sixteen articles were included in the analysis and classified into five categories: genetic factors, nutrition, smoking, pregnancy characteristics or complications, and disease-related characteristics.

Conclusions
Our meta-analysis revealed adolescent pregnancy, smoking, eclampsia, bacterial vaginosis, LC-PUFA, placental vitamin D, and several minerals as the significant determinants of preterm birth in urban Indonesia.
Disparities in preconception health indicators in U.S. women: a cross-sectional analysis of the behavioral risk factor surveillance system 2019
Authors: Terry Rachel , Gatewood Ashton , Elenwo Covenant , Long Abigail , Wu Wendi , Markey Caroline , Strain Shawn , Hartwell Micah 
Published: December 2023

Abstract

Objectives
Optimized preconception care improves birth outcomes and women’s health. Yet, little research exists identifying inequities impacting preconception health. This study identifies age, race/ethnicity, education, urbanicity, and income inequities in preconception health.

Methods
We performed a cross-sectional analysis of the Center for Disease Control and Prevention’s (CDC) 2019 Behavioral Risk Factor Surveillance System (BRFSS). This study included women aged 18–49 years who (1) reported they were not using any type of contraceptive measure during their last sexual encounter (usage of condoms, birth control, etc.) and (2) reported wanting to become pregnant from the BRFSS Family Planning module. Sociodemographic variables included age, race/ethnicity, education, urbanicity, and annual household income. Preconception health indicators were subdivided into three categories of Physical/Mental Health, Healthcare Access, and Behavioral Health. Chi-squared statistical analysis was utilized to identify sociodemographic inequities in preconception health indicators.

Results
Within the Physical/Mental Health category, we found statistically significant differences among depressive disorder, obesity, high blood pressure, and diabetes. In the Healthcare Access category, we found statistically significant differences in health insurance status, having a primary care doctor, and being able to afford a medical visit. Within the Behavioral Health category, we found statistically significant differences in smoking tobacco, consuming alcohol, exercising in the past 30 days, and fruit and vegetable consumption.

Conclusions
Maternal mortality and poor maternal health outcomes are influenced by many factors. Further research efforts to identify contributing factors will improve the implementation of targeted preventative measures in directly affected populations to alleviate the current maternal health crisis.