bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of gastrointestinal parasites of poultry in rural and peri-urban farms of Bobo-Dioulasso, Burkina Faso
Authors: ZONGO André , PAGABELEGUEM Soumaïla , SOMDA Martin Bienvenu , OUEDRAOGO Xavier Souleymane , DOULKOUM Daniel , BENGALY Zakaria , BELEM Adien Marie Gaston 
Published: November 2023

Abstract

The main objective of this study was to evaluate the helminth parasites encountered in poultry farms in the peri-urban and rural areas of Bobo-Dioulasso. A total of 160 fecal samples were collected from poultry breeding farms in 2 chicken breeds (exotic and local) and 2 areas (rural and peri-urban). The fecal samples was examined by the flotation method using saturated sodium chloride solution. McMaster technique was used to observe and quantify the oocysts of the different gastrointestinal parasites on the slide. The actors surveyed are all male literate with an average age of 48 ± 8.50 years. The average number held by the actors is 3023 ± 1865 chicken heads. They all have poultry farming as their main activity with an average of 12 ± 5.50 years, only 5% practice agriculture in addition to poultry activity. Of the 160 samples tested, 114 were positive, given an overall prevalence rate of 71.25%. The prevalence rate was significantly influenced by the area (𝜒2 = 11.06, df = 1, 𝑃 < 0.001) and the type of intestinal parasite (𝜒2 = 155.65, df = 2, 𝑃 < 0.001). Three types of gastrointestinal nematodes were responsible of these infections therefore Eimeria sp (53.13 ± 50.06%), Heterakis sp (43.13 ± 49.68%) and Ascaris sp (0.63 ± 7.91%). In positive samples, the number of parasites was found to be between 10 and 85000 for oocysts of Eimeria sp, 15 and 2300 for Heterakis sp and 100 for Ascaris sp. The majority of infections in urban area were caused by Eimeria sp (94.12%, p = 0.003), except on one farm where a Heterakis sp infection was encountered. However, in rural area, all intestinal parasites were encountered, with similar level of infections (p = 0.9) between Eimeria sp (50%) and Heterakis sp (49.30%), and which were significantly higher than infections due to Acaris sp (0.07%, p < 0.001). In rural area, mixed infections with Eimeria sp and Heterakis sp or Eimeria sp, Heterakis sp and Ascaris sp were observed in 41.24% and 1.03% respectively of the positive farms. The chicken breed has no significate on the prevalence (𝜒2 = 0.98, df = 1, 𝑃 = 0.3) but the local breed had the high prevalence of intestinal parasites of 77.92% as compared with exotic breed 65.06%. The results showed a high rate of intestinal parasites in poultry farm in rural and peri-urban areas of Bobo-Dioulasso. Therefore, further investigations and design appropriate control strategies in improving management of farms are necessary and strongly recommended.
Large-scale calcium imaging reveals a systematic V4 map for encoding natural scenes
Authors: Tang Shiming , Wang Tianye , Yao Haoxuan , Lee Tai Sing , Hong Jiayi , Li Yang , Jiang Hongfei , Andolina Ian 
Published: November 2023

Abstract

Biological visual systems have evolved to process natural scenes. A comprehensive understanding of visual cortical functions requires characterizing how natural stimuli are encoded by the massive population of neurons in each visual area. Here, we utilized widefield calcium imaging to sample primate V4 cortical response to tens of thousands of natural images. With this large dataset, we developed a deep-learning model of a substantial region of V4 that allowed us to map the natural image preference of neural populations at 100-µm scale. This map contains a variety of domains encoding distinct shape and surface features of natural scenes, including both previously reported attributes and previously missed ones like facial features or textures. We validated these model predictions with additional widefield calcium imaging and single-cell resolution two-photon imaging. Our study illuminates the V4 representation for natural scenes and provides an unbiased approach for characterizing the functional organization of primate visual cortex.
Investigation of the risk factors associated with prediabetes in normal-weight Qatari adults: a cross-sectional study
Authors: Elmagarmid Khadija , Fadlalla Mohamed Abbas , Jose Johane , Arredouani Abdelilah , Bensmail Halima 
Published: November 2023

Abstract
Background: Type 2 diabetes is one of the most prevalent chronic diseases in the world, and more people than ever before have impaired glucose tolereance, or prediabetes. Many patients with impaired glucose tolerance and undiagnosed diabetes do not know that their glucose metabolism system has been in a state of disorder. Every year, about 5%-10% of prediabetics develop diabetes. One of the important achieving factors may be the increase in blood lipids. However, it is not clear whether triglyceride is associated with impaired glucose tolerance and prediabetes in the Qatari population. Therefore, we investigated the relationship between the first several clinical variables and prediabetes status in normal and overweight populations.
Methods: We conducted a cross-sectional study using data from the Qatar Biobank program. The study included 5,996 participants who were adults over the age of 20. We collected information about participants’ fasting blood glucose levels with other clinical measurements and used various machine learning models and logistic regression to study the association between the clinical measurements and prediabetes for normal and over&obese weight groups.
Results: The use of several machine learning models showed that, after adjusting the potential confounding factors such as age and sex, Triglyceride has been demonstrated to be positively correlated with prediabetes, and there was a special population dependence phenomenon. Among them, non-obese people (p<0.05). The effect value and 95% confidence interval and OR of triglyceride on prediabetes was 2.79 and (e0.78, e1.28), respectively.
Analysing the factors influencing groundwater quality using the Leachate Pollution Index (LPI), Heavy Metal Pollution Index (HPI), and Partial Least Squares - Structural Equation Modelling (PLS-SEM) in the vicinity of an open dumping yard in Saduperi, Vellore, Tamil Nadu, India.
Authors: Venkatesh Arumugasamy Thangapandian , Rajkumar Sujatha , MASILAMANI UMA SHANKAR 
Published: November 2023

Abstract
Open dumping is the prevailing municipal solid waste (MSW) disposal technique in India. Unsanitary landfill system results in the release of leachate, a substance that has the potential to contaminate the nearby environment, including groundwater. Hence, the present study was carried out in the vicinity of the Saduperi open dumpsite, Vellore, Tamil Nadu, India, to explore the key factors that influence groundwater contamination. 18 sample wells were identified near the dumpsite and a total of 216 groundwater samples were collected between May 2021 and April 2022. These samples were categorized into four different seasons such as summer, southwest monsoon (SWM), northeast monsoon (NEM), and winter. The groundwater contamination is assessed using hydrogeochemical methods such as Piper and Gibbs diagrams. The leachate pollution index (LPI) and Heavy metal pollution index (HPI) were used to evaluate the contamination potential. The calculated LPI > 35 in all seasons indicates the prevailing poor environmental condition. It was observed that about 56% of the sampling site was affected by heavy metal concentrations such as Cd, Cr, and Ni. The HPI value was found to be more than the critical value of 100 in the 10 sampling wells for all seasons. Partial least squares-structural equation modelling (PLS-SEM) offers a novel approach to assessing the intricate link between several influencing elements and groundwater quality, in contrast to the conventional multivariate statistical technique. The PLS-SEM creates the link between Latent variables such as “IOT Parameters”, “Leachate Parameters”, “Heavy Metal” and “Groundwater Quality” which was quantified by the yield of R2 value. The R2 value of the sampling well ahead of the dumpsite and along the direction of the groundwater flow values ranging from 24.7–86.5% than the wells located behind the dumpsite are prone to get more contamination due to migration of leachate. Hence this present study shows various influencing factors that affect the groundwater quality.
Maternal and fetal outcomes in pregnant patients with systemic lupus erythematosus:a single center studyMaternal and fetal outcomes in pregnant patients with systemic lupus erythematosus:a single center study
Authors: Zeng Wen , Lei Ling , Zhang Lan , Zhao Cheng , Qin Fang , Chen Zhanrui , Wen Jing , Dong Fei , Wei Wanling 
Published: November 2023

Abstract
Objective: Identify risk factors associated with maternal and fetal outcomes in pregnant patients with systemic lupus erythematosus (SLE).
Methods: Retrospective analysis of the characteristics and pregnancy outcomes of 283 women with SLE.
Results: The mean age at delivery was 29.3±4.7 years, the mean SLE duration was 6.1±4.6 years, and 151 women (53.3%) had adverse pregnancy outcomes (APOs). The APO group had lower levels of C3 and C4, greater level of anti dsDNA antibodies, higher albumin-to-creatinine ratio (ACR), greater frequent history of lupus nephritis (LN), greater use of cyclosporine A (CsA), used higher doses of glucocorticoids (GCs), and had higher disease-activity scores (SLEDAI-2000; all p<0.05). Pregnancy loss (PL) and low birth weight (LBW) infants were also associated with several maternal factors. Multivariate logistic regression analysis showed that low C3 level and CsA use increased the risk of APO, and disease remission for more than 6 months was a protective factor (all p<0.05). A low C3 level increased the risk for PL, and CsA use increased the risk for premature birth (PB; both p<0.05). The dose of GC was associated with APO and PL (both p<0.05), with cut-off values of 8.5 and 9.5 mg/day, respectively.
Conclusion: Pregnant women with SLE who have a low level of C3, use CsA, and use higher doses of GCs have an increased risk of APO. Decreased C3 and use of higher doses of GCs increased the risk for PL, and CsA use increased the risk of PB. Pregnancy after 6 months of SLE remission was protective.
A novel defined ferroptosis-related gene signature for predicting the prognosis of Osteosarcoma
Authors: Pan Chongzhi , Huang Guanfeng , Zhu Wenjie , Chen Zhaojun , Liu Yuchi , Pan Huajun , Wu Tianlong , Cheng Xigao 
Published: November 2023

Abstract
Background
Osteosarcoma is a malignant tumor that is harmful to adolescents and has a poor prognosis. There is no effective treatment at now. This study aimed to construct a prognosis based on ferroptosis-related genes to provide guidance for the treatment of osteosarcoma.
Methods
In this study, we identified three ferroptosis-regulating genes that were distinctly expressed in osteosarcoma and normal tissues. Osteosarcoma patients from three datasets were classified using consensus clustering of these three ferroptosis-regulated genes, and gene differences among the three subtypes were analyzed in one step, and these differentially expressed genes were analyzed by univariate regression (DEGs), the ssGSEA method was used to assess the tumor microenvironment (TME), and the principal component analysis algorithm was used to quantitatively construct a predictive model. Finally, we use qRT-PCR to detect gene expression to validate the model.
Results
We identified three distinct ferroptosis genotypes, and the differences between the three subtypes were used to build a prognostic model, which we termed the "Ferroptosis-Score". The score can predict the prognosis and overall survival time of patients with osteosarcoma. The survival of patients with high scores is higher than that of patients with low scores (P < 0.05). At the same time, it is observed that immune cell infiltration in patients with high scores is significantly higher than that in patients with low scores.
Conclusion
We constructed a Ferroptosis-Score scoring system, the higher the score, the better the prognosis of the patient. And found that cluster C may activates ferroptosis through autophagy and JAK-STAT signaling pathway, so the patients of cluster C have better prognosis.
Quantifying post-saccadic macroscopic traveling brain waves in marmosets with graph-based algorithm
Authors: Chen Chih-Yang , HO KUAN-TING , Onoe Hirotaka , Isa Tadashi 
Published: November 2023

Abstract
Saccades are rapid eye movements that also strongly influence the visual processing. A variety of perisaccadic modulations of neuronal activity have been widely studied in many visual areas, yet the relationship between these modulations remains unclear. In this study, we recorded hemispheric electrocorticogram from marmosets performing visually-guided saccade task. We found that the 20 ~ 50 Hz phase-locked activities in the occipital cortex during 0 ~ 100 ms after saccade offset actually form a macroscopic traveling wave (TW) which originated from the primary visual area and propagated rostrally, occasionally to the parietal and temporal cortex. We designed an algorithm based on graph theory that can flexibly identify TWs and quantify wave parameters in data from large-scale recording systems. The results showed that latency, source, and other parameters of these TWs were modulated by saccade direction. We suggest this TW may function to sequentially reset the neuronal excitability along the cortical visual pathways after saccades.
Business Model Innovation of Chinese Logistics Enterprises from the Perspective of Ecosystems: The Case of Cainiao Network
Authors: Wang Jian , Si Fan , Yang Siyu , Wang Lin 
Published: November 2023

Abstract
With the development and application of digital technology, a new logistics ecology based on the internet and big data is emerging through collaborative innovation models and advanced Artificial Intelligence technology. Building on the event system theory, we analyze Cainiao’s business model innovation from the perspective of ecosystem research through three aspects: value proposition, value co-creation, and value capture. The research concludes that Cainiao should realize business model innovation from the following three aspects: (1) clearing value proposition, (2) co-creating value through ecological cooperation and an open platform, and (3) capturing value by positioning precisely, distributing legitimately, and managing the supply chain.
Distinct clinical characteristics and outcome caused by a novel deletion mutation in XIAP/BIRC4 gene: a case report
Authors: Wang Junyan , Ma Hanwei , Li Ye , Zhang Xiaoyan , Zhang Juanli 
Published: November 2023

Abstract
X-linked lymphoproliferative disease (XLP), as a disorder of the immune system and blood-forming cells, is closely related to the infection with the Epstein-Barr virus (EBV) in most cases. XLP was found almost exclusively in males and it was estimated that around two million of males in the world are affected by this disease. Although intensive research work has been conducted on revealing the symptoms, pathology and the relevant contributing factors of XLP, a review of the reported literature shows that the reports of the asymptomatic state associated XLP or full recovery after treatment are relatively rare. In this paper, a case of XLP-2 with a novel deletion mutation in China is reported and discussed. From the Sanger sequencing analysis, it is found that the XIAP deficiency was caused by the deletion mutation (c.993 (exon4) _c.997 (exon4) del AGACE) in the XIAP gene however this novel mutation had not been reported. The evidence found in this case report implies that EBV infection is still a key stimulant. Besides, it can be inferred from the analysis of the results that peculiar gene mutation may lead to better prognosis. *Corresponding author: Junyan Wang Email: jywang@lzu.edu.cn
Utility of Adrenal Vein Sampling (AVS) with and without ultra-low dose ACTH infusion in the diagnostic evaluation of Primary Aldosteronism
Authors: Preston Christopher , Yong Eric , Marginson Benjamin , Farrell Stephen , Sawyer Matthew , Hahimura Hikaru , Derbyshire Maresa , MacIsaac Richard , Sachithanandan Nirupa 
Published: November 2023

Abstract
Adrenal vein sampling (AVS), integral to identifying surgically remediable unilateral primary aldosteronism (PA), is technically challenging and subject to fluctuations in cortisol and aldosterone secretion. Intra-procedural adrenocorticotropic hormone (ACTH), conventionally administered as a 250mcg bolus and/or 50mcg per hour infusion, increases cortisol and aldosterone secretion and can improve AVS success, however, may mask lateralisation of unilateral PA. Here we describe our AVS experience using an ultra-low dose ACTH infusion protocol. We retrospectively reviewed the results of consecutive AVS procedures (n = 37) performed with and without ultra-low dose ACTH (1mcg bolus followed by 1.25mcg per hour infusion). Bilateral AV cannulation was successful in 70% of procedures pre-ACTH and 89% post-ACTH (p < 0.01). Sixty-nine percent of studies lateralised pre-ACTH and 55% post-ACTH, improving to 79% when both groups were combined. Lateralisation was discordant in 11 cases, including eight in which ACTH masked lateralisation, and three in which lateralisation occurred only with ACTH stimulation. Overall, the decrease in lateralisation rates with ACTH was similar to previously reported rates for high dose protocols. Of 21 operated patients, 86% had an adrenal adenoma and 10% had adrenal hyperplasia on histology. Our results suggest that AVS performed with ultra-low dose ACTH masks lateralisation and does not obviate the need for non-ACTH AVS. We conclude, combined AVS with and without ultra-low dose ACTH improved the overall diagnostic yield of the procedure, identifying additional cases of unilateral PA. Studies directly comparing low and high dose ACTH AVS protocols may help define the optimal ACTH dose for accurate PA subtyping.
Seroprevalence and risk factors of STIs among rejected blood donors at the National Blood Transfusion Service in Angola
Authors: Sebastião Cruz S. , Jandondo Domingos , Vigário António , António Felícia , Vienga Pedro , Sebastião Joana , Pimentel Victor , Abecasis Ana , Sacomboio Euclides , Vasconcelos Jocelyne , Morais Joana 
Published: November 2023

Abstract
Background
Sexually transmitted infections (STIs) are a global health concern. Blood donation centres employ comprehensive screening measures to identify donors with STIs, including HIV, HBV, HCV, and syphilis ensuring the safety of the donated blood and protecting the recipients. Herein, we investigated the seroprevalence of STIs and risk factors related to multiple infections among rejected blood donors at the National Blood Transfusion Service (NBTS) in Angola.
Methods
This was a cross-sectional study conducted with 1668 rejected blood donors serologically screened for anti-HBsAg, anti-HCV, anti-HIV, and anti-TP at the NBTS, located in Luanda, the capital city of Angola, between March 2022 to July 2023.
Results
Overall, HIV (11.2%), HBsAg (71.7%), HCV (9.30%), and Syphilis (8.80%) were detected. The multiple infection rate was 2.30%, from these, HIV/HBV (1.10%), HIV/HCV (0.60%), HIV/Syphilis (1.20%), HBV/HCV (8.0%), HBV/Syphilis (5.50%), and HCV/Syphilis (1.80%) coinfections, were detected. HIV and syphilis were related to age groups (p < 0.05). HBV was related to age group, areas of residence, occupation, and Rh factor (p < 0.05). HCV was related to areas of residence and educational level (p < 0.05). No relationship was observed between demographic characteristics with multiple infections (p > 0.05). Individuals aged over 40 years (OR: 2.48, p = 0.393), males (OR: 1.33, p = 0.639), non-urbanized regions (OR: 1.18, p = 0.594), low educational level (OR: 3.46, p = 0.222), and employed (OR: 1.34, p = 0.423), presented a higher likelihood of having multiple infections.
Conclusions
Our results demonstrate a high rate of circulation of STIs among young blood donation candidates in Luanda. However, the prevalence, particularly that of HBV, is one of the highest in the sub-Saharan African region, suggesting that priority should be given to strengthening donor screening and considering the possibility of including nucleic acid-based screening techniques.
Coliform bacterial contamination of dairy products and antimicrobial susceptibility patterns of common coliform bacterial isolates in Gondar-Bahir Dar milk shed, Northwest Ethiopia
Authors: Beyene Achenef Melaku , Gizachew Mucheye , Yousef Ahmed , Jemere Zenebe , Haileyesus Hana , Gelaw Baye 
Published: November 2023

Abstract

Background: Dairy products can be contaminated by pathogenic microorganisms due to unhygienic production and processing practices. Determining the status of the bacteriological contamination is vital to design evidence-based strategies to minimize the risk of infections and to enhance the quality and shelf life of processed products. This study was conducted to determine the status of contamination of dairy products with coliform bacteria and the antimicrobial susceptibility of common coliform bacterial isolates in the Gondar-Bahir Dar milkshed, Northwest Ethiopia.
Methods: In total, 184 dairy product samples (raw milk (97), yoghurt (traditionally fermented milk) (48), buttermilk (9), traditionally prepared soft cheese (11), and pasteurized milk (19)) were collected. Of these, the coliform count was conducted on 146 samples (raw milk (97), yoghurt (6), buttermilk (6), traditionally prepared soft cheese (11), and pasteurized milk (19)) was assessed by coliform counts. Coliform bacteria were isolated from all 184 samples using standard bacteriological techniques and isolates were identified by the Analytical Profile Index (API) E 20 test. The antimicrobial susceptibility of the bacterial isolates was assessed by using the Kirby-Bauer disk diffusion method.
Results: The average coliform count (CC) of samples was 5.6 log CFU/ml (confidence interval (CI)= 5.3 – 6.0). There was a significant (P<0.05) difference in the average CC among sample types, cleanness of the animals and hygiene of the containers. However, the differences in the average CC among sampling sites, farm size, source of water to clean milk containers, availability of cooling facilities, feed source, type of container, farm hygiene, and milker’s hygiene were not statistically significant. Out of examined 184 samples, 40.8, 33.7, 9.2, 12.5, and 1.6% were positive for E. coli, Citrobacter species (spp), Klebsiella spp, Enterobacterspp, and Serratia spp, respectively. Coliform bacteria isolates were more susceptible to third-generation cephalosporins like ceftazidime, ceftriaxone, and cefotaxime. However, they were relatively more resistant to cephalothin and ampicillin.
Conclusion: most of the dairy product samples were highly contaminated by coliform bacteria and the isolates demonstrated a considerable rate of resistance. Therefore, hygienic measures should be enhanced to improve the bacteriological quality of dairy products in the study area, and strategies need to be designed to reduce the proliferation and spread of resistant strains to minimize public health risks.