bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunolocalisation of angiotensin II receptors in icefish (Chionodraco hamatus) tissues
Authors: Marsigliante S , Acierno R , Maffia M , Muscella A , Vinson G P , Storelli C 
Published: December 2004

Abstract
The monoclonal antibody 6313/G2 raised against the mammalian type I (AT1) angiotensin II (Ang II) receptor (Ang II-R) also recognises a component in teleost (eel) tissue preparations that binds radiolabelled Ang II, and has an isoelectric point (pI) of 6·5 and molecular mass of 75 kDa. Immunohistochemical analysis using this antibody showed specific binding sites in eel intestine, kidney, gill and liver sections.
The same antibody was used here to evaluate the presence and distribution of Ang II-R in target tissues of the Antarctic teleost icefish (Chionodraco hamatus).
Immunocytochemistry of intestine and gill sections showed that the antibody bound to uniformly distributed intracellular sites and cell surface membranes in absorptive cells in the intestine and chloride and pavement cells in the gills. It also stained endothelium and both the longitudinal and circular layers of smooth muscle cells in the intestine. In the kidney, only the tubules in the trunk stained positively while the head (atubular part of the kidney) was negative. In kidney tubules, in contrast with other tissues, the receptor was most concentrated in the cytoplasm underlying the basolateral membranes, with somewhat weaker staining beneath the apical cell membrane. Immunoblotting identified a single component from trunk kidney preparations that focused at pI 5·9 in isoelectric focusing gels and showed a molecular mass of 75 kDa in SDS–polyacrylamide gels.
The data suggest that, as in other teleosts, Ang II has a physiological role in the icefish.
Journal of Endocrinology (1997) 154, 193–200
Luteotrophic factors in hyperstimulated pseudopregnant rabbit: II – High sensitivity to hCG of luteal tissue and small luteal cells
Authors: Arioua R K , Benhaïm A , Féral C , Leymarie P 
Published: December 2004

Abstract
Previous studies on rabbit corpus luteum (CL) led to the conclusion that the luteotrophic complex, in rabbit, may include LH as well as oestradiol for normal luteal function. However, the requirement for LH is controversial. We have recently demonstrated the existence of a human chorionic gonadotrophin (hCG)-stimulated aromatase activity in cultured corpora lutea from a hyperstimulated pseudopregnant rabbit model, which develops a large number of corpora lutea, with only a few or no follicles in the ovaries. The present study was undertaken to investigate the in vitro responsiveness to hCG, dibutyryl cAMP (dbcAMP) and oestradiol of those corpora lutea.
Pseudopregnancy (PP) was induced in rabbits by i.m. injection of 200 IU equine chorionic gonadotrophin daily for 2 days followed on day 4 by i.m. injection of 200 IU hCG (day 0 of PP). Luteal tissue and small and large luteal cells obtained at days 5 and 9 of PP were cultured for 24 h or during several days respectively with or without hCG, dbcAMP or oestradiol. Basal progesterone secretion was 3·6- and 22-fold higher in large cells compared with small ones at day 5 and 9 of PP respectively. When stimulated by small doses of hCG, luteal tissue responded by a 5-fold increase in progesterone secretion. Small cells produced four times higher amounts of progesterone than controls in the presence of 1 mIU/ml hCG and more than ten times in the presence of 0·1 IU/ml hCG, whereas large cells were insensitive to hCG stimulation. dbcAMP mimicked the effect of hCG on progesterone secretion by luteal tissue and luteal cells and oestradiol stimulated basal progesterone secretion in both small and large luteal cells. Given the large contribution of non stimulated large cells to luteal progesterone production and the remarkably high sensitivity of luteal tissue to gonadotrophin in vitro it seems that interactions between the two types of cells might occur during LH stimulation. Our results suggest that LH could participate in the luteotrophic complex at least in part through the stimulation of endogenous oestradiol production by luteal cells.
Journal of Endocrinology (1997) 154, 259–265
Differential regulation of tissue insulin-like growth factor-binding protein (IGFBP)-3, IGF-I and IGF type 1 receptor mRNA levels, and serum IGF-I and IGFBP concentrations by growth hormone and IGF-I
Authors: Lemmey A B , Glassford J , Flick-Smith H C , Holly J M P , Pell J M 
Published: December 2004

Abstract
The aims of this investigation were (1) to examine IGF-binding protein-3 (IGFBP-3) mRNA levels in candidate tissues which might be important sources for blood IGFBP-3 (liver and skin) and in a target tissue for IGF-I action (skeletal muscle), and (2) to examine the effects of a single dose (500 μg) of GH or IGF-I on IGFBP-3 message levels in these tissues since temporal responses (4, 8 and 24 h after the single subcutaneous dose of peptide to GH-deficient dwarf rats) would indicate which peptide is the primary modulator of IGFBP-3 synthesis. Circulating IGF-I and IGFBP-3 concentrations were significantly increased (P<0·05) by IGF-I and GH. GH treatment increased liver IGFBP-3 mRNA levels by 4 h (P<0·001 over the 24 h) whereas IGF-I had no effect. Similarly, GH, but not IGF-I, increased muscle IGFBP-3 mRNA levels (P<0·001 for the 24 h study period). However, both IGF-I and GH induced increases in skin IGFBP-3 mRNA abundance throughout the 24 h period (P<0·001 and P<0·01 respectively) and skin IGFBP-3 message abundance was greater that in the liver. Liver IGF-I mRNA levels were, as expected, increased after GH and tended to decrease after IGF-I treatment; muscle IGF-I mRNA was increased by GH (P<0·001) and, interestingly, progressively increased by IGF-I (P<0·05 for the 24 h period); skin IGF-I mRNA levels were unchanged by both peptides. The IGF-I induced increase in serum IGFBP-3 concentrations in the absence of an increase in hepatic IGFBP-3 mRNA levels and a paucity of liver IGF-I type 1 receptor mRNA imply that other sources of IGFBP-3 protein or synthesis must exist. The response of skin IGFBP-3 mRNA levels to both GH and IGF-I suggests that other cell types, such as fibroblast-derived cells, could be more important than the liver in the regulation of circulating reservoir IGFBP-3 in certain circumstances. In contrast to some current suggestions, the rapid and consistent GH-induced increase in IGFBP-3 message levels in all tissues studied implies that GH might have a direct function in the regulation of IGFBP-3 synthesis.
Journal of Endocrinology (1997) 154, 319–328
Renal responses to oxytocin during the 4 days of the oestrous cycle in the rat
Authors: Windle R J , Forsling M L 
Published: December 2004

Abstract
Oxytocin was administered to virgin female rats at doses of 25–200 pmol/min during 0·077 mol NaCl/l infusion at 150 μl/min on each day of the oestrous cycle. The resultant rates of urine flow, glomerular filtration (GFR) and electrolyte excretion were determined. Oxytocin caused significant increases in urine flow (P<0·001) and sodium excretion (P<0·001); both responses being dose-dependent (P<0·02 and P<0·01 respectively). Significant variations in the renal responsiveness to the hormone occurred over the 4 days of the oestrous cycle. On oestrus the lowest dose of 25 pmol oxytocin/min produced a significant increase in urine flow (from 139·5 ± 4·3 to 165·6 ± 7·1 μl/min, P<0·005) and a dose of 50 pmol/min produced a significant increase in sodium excretion (from 10·6 ± 0·1 to 14·5 ± 0·7 μmol/min, P<0·005). Significant increases in urine flow and sodium excretion were seen on pro-oestrus with hormone administration rates of 50 and 100 pmol/min respectively and on dioestrus day 2 with a rate of 100 pmol/min. On dioestrus day 1 no increase in urine flow or sodium excretion was seen over the dose range of oxytocin administration. A dose of 100 pmol oxytocin/min significantly increased GFR on pro-oestrus and dioestrus day 2, but not on the other 2 days of the cycle. The circulating hormone concentrations produced by oxytocin infusion were similar on each day of the cycle and so could not account for the differences seen. Therefore, these results suggest varying renal responsiveness to oxytocin during the reproductive cycle of the female rat.
Journal of Endocrinology (1997) 154, 347–353
Detection of serum insulin-like growth factor binding proteins on Western ligand blots by biotinylated IGF and enhanced chemiluminescence
Authors: Op De Beeck L , Verlooy J E A , Van Buul-Offers S C , Du Caju M V L 
Published: December 2004

Abstract
A novel procedure for the detection of IGF binding capacity of IGFBPs on Western ligand blots (WLB) was developed using biotinylated IGFs as probes. The biotinylated IGF-IGFBP complexes were visualized by streptavidin-horseradish peroxidase and enhanced chemiluminescence (ECL). The procedure was found to be faster and more efficient than the conventional method with iodinated IGFs. In normal human serum a predominant doublet at 38-42 kDa and five smaller bands at 35, 34, 30, 28 and 24 kDa were detected by both methods, whereas two additional bands at 26 and 16 kDa became visible with the ECL method. In pregnancy serum only one single faint band at 30 kDa could be detected by the iodinated method. In contrast, the ECL method revealed five other bands at 42, 34, 28, 26 and 16 kDa. Besides the 38-42 kDa doublet, the 30 and 16 kDa bands reacted strongly with anti-IGFBP-3 antibodies in Western immunoblotting (WIB) and therefore were related to IGFBP-3 fragments. The technical advantages of this ECL method include an extremely short exposure time to the radiographic film and a long stability of the probe. In addition, the ECL method is a non-radioactive method, making radioprotection and radioactive waste removal unnecessary.
Diurnal changes in hypothalamic neuropeptide and SOCS-3 expression: effects of lactation and relationship with serum leptin and food intake
Authors: Denis R G P , Bing C , Brocklehurst S , Harrold J A , Vernon R G , Williams G 
Published: December 2004

Rats normally eat about 85% of their food at night. Lactation increases food intake 3- to 4-fold, but the diurnal pattern of food intake persists. The mechanisms responsible for the diurnal and lactation-induced changes in food intake are still unresolved, hence we have further investigated the possible roles of serum leptin and hypothalamic expression of neuropeptide Y (NPY), agouti-related peptide (AgRP) and pro-opiomelanocortin (POMC) in rats. Suppressor of cytokine signalling-3 (SOCS-3) acts as a feedback inhibitor of leptin signalling in the hypothalamus, hence changes in expression of SOCS-3 were also investigated.
Changes in expression of NPY, AgRP or POMC alone could not account for the diurnal changes in intake and their alteration by lactation. However, there were increased AgRP mRNA:POMC mRNA ratios at night and also during lactation, which were very similar to estimated changes in food intake. Such changes in expression may result in dominance of the orexigenic AgRP peptide over the appetite-suppressing POMC-derived peptides, and so could contribute to the hyperphagia in these states. Diurnal and lactation-related changes in the AgRP mRNA:POMC mRNA ratio and food intake are not due to changes in leptin alone. However, hypoleptinaemia, possibly through increased expression of NPY, may contribute to the hyperphagia of lactation.
In the dark, expression of SOCS-3 was decreased in non-lactating rats; lactation decreased SOCS-3 expression in both light and dark phases. However, such changes are likely to enhance the ability of leptin-responsive neurones to transmit the leptin signal, and so are unlikely to contribute to either the nocturnal increase in appetite or the hyperphagia of lactation.
Studies of the neuromedin U-2 receptor gene in human obesity: evidence for the existence of two ancestral forms of the receptor
Authors: Bhattacharyya Sumit , Luan Jian’an , Farooqi I Sadaf , Keogh Julia , Montague Carl , Brennand John , Jorde Lynn , Wareham Nicholas J , O’Rahilly Stephen 
Published: December 2004

Central administration of neuromedin U (NMU) suppresses food intake acting through the NMU-2 receptor (NMU2R), which is expressed in the hypothalamus. We screened the NMU2R gene in 96 patients with severe early-onset obesity. A common variant haplotype was found (f-0.21). This common variant haplotype was unusual in nature, consisting of four non-contiguous missense changes in complete linkage disequilibrium, and across two separate exons. The variant haplotype resulted in four amino acid substitutions (S295T/F312L/P380L/ M385 V) and was present in several other Europid populations and in subjects of South Asian, East Asian and African American origin, but not in eleven African Pygmies. This variant haplotype was not associated with obesity or related traits in 500 subjects from a prospective population-based cohort.
In summary, we have identified two markedly different isoforms of the NMU-2 receptor, presumably arising through an ancient and complex mutational event; no genetic associations between this haplotype and obesity-related traits were, however, discerned. Further investigation of the pharmacogenomic consequences of NMU2R variation in humans is warranted.
Comparison of the Antibacterial Activity of Selected Deep Eutectic Solvents (DESs) and Deep Eutectic Solvents Comprising Organic Acids (OA‐DESs) Toward Gram‐Positive and Gram‐Negative Species
Authors: Swebocki Tomasz , Kocot Aleksandra M. , Barras Alexandre , Arellano Helena , Bonnaud Leila , Haddadi Kamel , Fameau Anne‐Laure , Szunerits Sabine , Plotka Magdalena , Boukherroub Rabah 
Published: February 2024

AbstractDeep eutectic solvents (DESs) have been intensively investigated in recent years for their antibacterial properties, with DESs that comprise organic acids (OA‐DESs) showing promising antibacterial action. However a majority of the reports focused only on a limited number strains and techniques, which is not enough to determine the antibacterial potential of a substance. To bridge this gap, the antibacterial activity of classical DESs and OA‐DESs is assessed on twelve Gram‐negative and Gram‐positive bacteria strains, with some of them exhibiting specific resistance toward antibiotics. The investigated formulations of OA‐DESs comprise glycolic, malic, malonic, and oxalic acids as representatives of this group. Using a range of microbiological assays as well as physicochemical characterization methods, a major difference of the effectiveness between the two groups is demonstrated, with OA‐DESs exhibiting, as expected, greater antibacterial effectiveness than classical DESs. Most interestingly, slight differences in the minimum inhibitory and bactericidal concentration values as well as time‐kill kinetics profiles are observed between Gram‐positive and Gram‐negative strains. Transmission electron microscopy analysis reveals the effect of the treatment of the bacteria with the representatives of both groups of DESs, which allows us to better understand the possible mechanism‐of‐action of these novel materials.
Multifaceted roles and functions of SOX30 in human cancer
Authors: Sun Na , Wang Cheng , Gao Pingping , Wang Rui , Zhang Yi , Qi Xiaowei 
Published: February 2024

AbstractSRY‐box transcription factor 30 (SOX30) participates in tumor cell apoptosis in lung cancer. The occurrence of somatic SOX30 mutations, the expression signature of SOX30 in normal and cancer tissues, the correlation of SOX30 with immune cells and immune‐related genes, and the clinical significance of SOX30 in various cancers have stimulated interest in SOX30 as a potential cancer biomarker. SOX30 influences drug sensitivity and tumor immunity in specific cancer types. In this review, we have comprehensively summarized the latest research on the role of SOX30 in cancer by combining bioinformatics evidence and a literature review. We summarize recent research on SOX30 in cancer regarding somatic mutations, trials, transcriptome analysis, clinical information, and SOX30‐mediated regulation of malignant phenotypes. Additionally, we report on the diagnostic value of SOX30 mRNA expression levels across different cancer types. This review on the role of SOX30 in cancer progression may provide insights into possible research directions for SOX30 in cancer and a theoretical basis for guiding future studies.
Macroeconomic conditions and bank failure
Authors: Wu Qiongbing , Cole Rebel A. 
Published: February 2024

AbstractUtilizing a simple time‐varying hazard model, we incorporate nationwide and state‐level economic variables with banking‐industry and bank‐level data to examine U.S. bank failures during 1977–2019. We find that bank‐level financial conditions are more essential in predicting bank failure, although macro factors affect the failure likelihood of vulnerable banks. We also find that banking‐industry market variables are significant predictors. Unlike bank systemic funding cost whose predictive power is subsumed in the presence of macroeconomic variables, banking‐industry market performance has a significantly independent predictive power on bank failure. This finding is novel to existing literature of bank‐failure forecast and has important policy implications.
From intention to action: Assessing need and creating a JEDI toolkit for individuals teaching cancer genetics curriculum
Authors: Paysour Jamie , Shehayeb Susan , McVayre Kelsie , Garg Shreshtha , Amir Fatima , Vercruyssen Anna , Sitaula Alina , Gerhart Meredith , Greenberg Samantha E. 
Published: February 2024

AbstractThe effects of systemic racism persist in cancer care and contribute to disparities. Recent publications have shown that injustices and biases continue to affect the field of genetic counseling in the form of microaggressions, barriers to entry, and disparate patient care. Toolkits are one method that can be used to incorporate anti‐racist practices to address this need. We sought to identify the current state of coverage of Justice, Equity, Diversity, and Inclusion (JEDI) topics during cancer genetics training across genetic counseling training programs (GCTPs) and utilize this information to create a novel toolkit that would support integration of anti‐racist pedagogy into formal genetic counseling curricula. To accomplish this aim, recent learners and program directors/cancer course instructors were surveyed using two novel surveys. The survey responses, which helped to identify the frequency and manner of incorporation of JEDI topics into cancer curricula in GCTPs, led to the development of an educational toolkit. Recent learners and instructors/program directors identified multiple content areas within cancer genetic training in which they felt incorporating JEDI topics would be desired. A toolkit to support the incorporation of anti‐racist teaching and practices into cancer genetics training in GCTPs was created. This toolkit can be adapted to focus on topics relevant to the care of other marginalized identities and to support the learning of other healthcare providers receiving cancer genetics education.
Climatology of icing conditions over Colombia based onERA5reanalysis and in situ observations
Authors: Chicaeme‐Ordoñez Kevin , Baquero‐Bernal Astrid , Mejía John F. 
Published: February 2024

AbstractThis study shows vertical profiles and spatial distribution of upper‐air icing frequency over the tropical Americas. We estimated the in‐flight icing (IFI) over Colombia using the Current Icing Product‐sonde‐A algorithm over two data sets: (1) vertical soundings of temperature and relative humidity and surface station data taken at 12 Coordinated Universal Time or UTC (07 Local Time or LT) on five sites and (2) ERA5 at 00, 06, 12 and 18 UTC (19, 01, 07 and 13 LT). In either case, icing was defined for IFI values exceeding 0.01. Results show that icing tends to occur between 550 and 300 hPa (4.5 and 8.6 km altitude), with a maximum at 500–550 hPa and monotonically decreasing to zero until reaching 300 hPa. Aeronautic reports were used to evaluate the total column IFI and a layer‐based IFI detection with a probability of detection of 87% and 71%, respectively. The annual cycle of IFI is modulated by the meridional migration of the Intertropical Convergence Zone (ITCZ) with a bimodal distribution with peaks during the rainiest seasons. Spatially, IFI hotspots are found in the Pacific, the Andes Mountains and the Amazonia regions of Colombia; the northern Colombia Caribbean region show lower IFI frequency with a relative maximum collocated over the Sierra Nevada de Santa Marta mountains. The IFI exhibits a strong diurnal cycle with a high between night‐time to early morning and a low around noon.
Highlights in the clinical nutrition literature: A critical appraisal of current research
Authors: Stoppe Christian , Elke Gunnar , Silvstre Simone Chaves de Miranda , Kappus Matthew 
Published: February 2024

AbstractWithin the American Society for Parenteral and Enteral Nutrition (ASPEN), the Physician Engagement Committee (PEC) was created in 2017 by the ASPEN Board of Directors with the goal of growing the physician community both nationally and internationally. The PEC meets each month throughout the year to develop educational and research initiatives. In 2022, the PEC began an initiative to systematically review and evaluate practice‐changing literature annually with the overall aim to highlight these studies at the annual ASPEN conferences and to critically discuss the potential clinical implications. The objective of the held meeting session was to present identified key papers in the fields of critical care medicine, gastroenterology and hepatology, and adult internal medicine that were published in 2022, which would complement the knowledge of the pathogenesis, diagnosis, and management of nutrition topics as well as to identify areas of future research. Overall, several large‐scale randomized controlled studies were identified in each of these sections, with practice‐changing major results. This manuscript summarizes the information that was presented and the discussions that followed.
An Exploratory, Randomized, Double‐Blind Clinical Trial of Dipraglurant for Blepharospasm
Authors: Kilic‐Berkmen Gamze , Kim Hodam , Chen Dongdong , Yeo Cameron I. , Dinasarapu Ashok R. , Scorr Laura M. , Yeo Woon‐Hong , Peterson David A. , Williams Hilde , Ruby April , Mills Roger , Jinnah H. A. 
Published: February 2024

AbstractBackgroundBlepharospasm is treated with botulinum toxin, but obtaining satisfactory results is sometimes challenging.ObjectiveThe aim is to conduct an exploratory trial of oral dipraglurant for blepharospasm.MethodsThis study was an exploratory, phase 2a, randomized, double‐blind, placebo‐controlled trial of 15 participants who were assigned to receive a placebo or dipraglurant (50 or 100 mg) and assessed over 2 days, 1 and 2 hours following dosing. Outcome measures included multiple scales rated by clinicians or participants, digital video, and a wearable sensor.ResultsDipraglurant was well tolerated, with no obvious impact on any of the measurement outcomes. Power analyses suggested fewer subjects would be required for studies using a within‐subject versus independent group design, especially for certain measures. Some outcome measures appeared more suitable than others.ConclusionAlthough dipraglurant appeared well tolerated, it did not produce a trend for clinical benefit. The results provide valuable information for planning further trials in blepharospasm. © 2024 International Parkinson and Movement Disorder Society.