bluuebunny/crossref_metadata_2025_split huggingface dataset:
#391 : Oocyte Cryopreservation — A Five Year Follow Up Study on its Utilization and Outcome
Authors: Baid Rashmi , Pai Hrishikesh D , Palshetkar Nandita 
Published: January 2024

Background & Aim: Oocyte cryopreservation is a boon for women undergoing Assisted reproductive technology (ART). Apart from cancer patients it is recently promoted as a mode of fertility insurance to overcome the age-related decline in fertility, endometriosis, primary ovarian insufficiency, etc. The objective of the study was to evaluate the clinical applications of oocyte freezing and its efficacy in terms of pregnancy outcome. Method: Retrospective observational study of all patients undergoing oocyte cryopreservation from 2016 to 2021 in Lilavati Hospital & Research Centre, Mumbai, India. Result: 221 patients undergoing oocyte cryopreservation were included. Oocyte cryopreservation was done for different indications like azoospermia (50.2%, most common), planned oocyte freezing (41.17%), fertility preservation (1.36%), endometriosis (0.45%), ejaculatory dysfunction (1.8%), husband with active hepatitis B infection (3.62%) and unavailability of husband on the day of egg retrieval (3.62%). A total of 1715 mature oocytes were frozen. 41.17% women were normo-responder, 38.91% were poor responder and 19.90% were hyper-responder. The oocytes of 59 patients (return rate-26.7%) have been thawed and 162 women (73.3%) remain in storage. Out of 508 oocytes frozen for these 59 patients utilizing their oocytes, around 474 oocytes were recovered post-warming (recovery rate 93.3%). A total of 59.3% patients created embryos with a partner, and 40.6% used donor sperm. The mean fertilization rate was 65.8% of surviving oocytes. Of 59 patients, 44 (74.5%) planned a fresh embryo transfer, out of which 20 patients tested positive for pregnancy (pregnancy rate 45.45%). Conclusion(s): We report the outcomes of patients opting for oocyte cryopreservation, which is useful for patient counselling. Limited evidence is available in terms of utilization rate, post warming survival of oocytes, fertilization and pregnancy rate. Further studies over long period of time with larger cohorts are needed.
#5 : Associated Factors with Premenstrual Syndrome and Premenstrual Dysphoric Disorder Among Female Medical Students: A Cross-Sectional Study
Authors: Trieu Vy Ngo Dinh , Bui Linh Phuong , Hoang Long Bao , Tran My Thi Tra , Nguyen Huy Vu Quoc , Tran Linh Manh , Pham Tung Thanh 
Published: January 2024

Background and Aims: Premenstrual syndrome (PMS) and Premenstrual dysphoric disorder (PMDD) are two premenstrual disorders that have been reported in many countries and have gradually become predominant concerns The study aimed to determine potential risk factors associated with PMS and PMDD Method: Three hundred two female student participants who were 18 – 45 years old completed a questionnaire including demographic characteristics, lifestyle factors, and a Vietnamese Premenstrual Syndrome Screening Tool (PSST). We then followed up participants during at least two menstrual cycles using the Daily Record of Severity of Problems (DRSP). The PMS and PMDD diagnosis were established using The Carolina Premenstrual Assessment Scoring System (C-PASS), based on the American College of Obstetrics and Gynecology (ACOG) and Diagnostic and Statistical Manual of Mental Disorders (DSM-5). Results: According to the C-PASS, 35 out of 302 students (11.6%; 95%CI: 8.2-15.7%) met the diagnosis of PMS (31 students) or PMDD (4 students). We found that age at menarche (PR = 0.77, 95%CI: 0.63 - 0.96), having negative Rh blood type (PR=4.43, 95%CI: 1.95 to 10.08), being moderately depressed or higher (PR=2.81, 95%CI: 1.24 to 6.36), and consuming caffeine more than three times per week were statistically associated with having PMS/PMDD after adjusting for other variables. Conclusion: The prominent risk factors for PMS and PMDD were negative Rhesus blood type, menarche age, caffeine consumption, and self-reported depression.
#94 : Study to Improve Clinical Results of Male Infertility Treatment ROSI (Round Spermatid Injection) with Correcting Genetic Expression by Histone Deacetylase Inhibitor
Authors: Tanaka Atsushi , Takemoto Youichi , Nakajima Daisuke , Nagayoshi Motoi , Tanaka Phar.B. Izumi , Watanabe Seiji 
Published: January 2024

Background and Aims: While the histone deacetylase inhibitor Scriptaid has been reported to be effective to improve ROSI results, there are also reports that offspring born after its use are stunted. In order to examine the conflicting results of basic research, experiments were conducted using mice, and the results are reported herein. Method: B6D2F1 mice were used, with Scriptaid liquid at 250nM in 10% SPS-HTF and 5mM strontium chloride, and Gx-TL (Vitrolife) was used as the embryo culture medium. Comparisons were made among three groups: (1) immersion of round spermatid cells in Scriptaid solution for 10 minutes before ROSI, (2) immersion of oocytes in Scriptaid solution for 16-18 hours after ROSI, and (3) conventional method without using Scriptaid. Results: No abnormalities were observed in the newborn offspring from all three groups, and their growth curves were normal at 10 weeks of age. There were no signs of stunting, which had been a concern. No abnormalities were found in the offspring born to the first generation. Conclusion: In basic animal experiments using the histone deacetylase inhibitor Scriptaid, normal results were obtained. The Ministry of Health, Labour and Welfare requires submission of an application for basic experiments using human gametes for ROSI. The problem is the judgment that the histone deacetylase inhibitor directly affects nucleic acids, but the histones targeted by the inhibitor are nucleosomes, not nucleic acids. Furthermore, the inhibitor does not target gene expression specific to the histones, and therefore does not constitute genome editing by definition. After obtaining approval for basic experiments, we aim to conduct basic experiments using human gametes and accumulate data on the risks and safety of this method.
#199 : Does Hysteroscopic Evaluation of Uterine Characteristic Improve Outcome of Thin Endometrium in Patients with a History of Repeated Implantation Failures?
Authors: Namshir Narangerel 
Published: January 2024

Background: Repeated implantation failure (RIF) is a problem for many patients suffering from infertility and their physicians. Despite numerous developments in the field of assisted reproduction, the implantation rate remains low. Among the various reasons of implantation failure, endometrial regularity has an important role. Recently, hysteroscopy is an accurate method for evaluating the endometrial integrity, with the ability to treat uterine pathology for repeated implantation failure patients. Objective: The aim of this study to evaluate the incidence of uterine pathology among infertile women with a history of two implantation failures after IVF and estimate the effect of diagnostic hysteroscopy and hysteroscopic procedures in uterine cavity on achieving a pregnancy in these patients in Mongolia. Materials and Methods: The retrospective study population included infertile women who attended the outpatient department of the Creation and Love Women’s infertility clinic, between August 2020 and July 2022 in Mongolia. The study inclusion criteria were women who had more than two RIF of IVF/ET cycle. Pregnancy and nonpregnancy rate after hysteroscopic procedures were analyzed included in patients reports archived in clinical data. Results: Hysteroscopy were performed on 35 women who had at least two RIFs. The participants’ mean age was 40.3±5.3 and mean duration of infertility year was evaluated 5.53±3.7. Mean AMH level was 1.4±1.6 ng/ml. All patients were diagnosed abnormal uterine cavity by hysteroscopy. Out of 35 patients participated in this study who underwent hysteroscopic evaluation, 20 patients (57.1%) showed synechiae (mild, moderate and severe synechiae). Second most frequent hysteroscopic finding was the endometrial polyp, accounting for 15 (42.9%) patients. Pregnancy rate after hysteroscopic procedures was 8 patients (23%) in this study group. The duration of infertility year was significantly different in pregnant and nonpregnant women underwent hysteroscopic correction (p<0.05). Conclusion: The intrauterine pathology diagnosed by hysteroscopy in patients with previous IVF failure were found all patients in this study. The synechiolysis was common abnormality in Mongolia may cause by artificial abortion. The correction of abnormalities such as and polyp synechiolysis might improve the outcome of successful pregnancy rate.
#306 : Enhancing Myoma Detection in Robot-Assisted Myomectomy: Evaluating the Efficacy of Intracorporeal Ultrasonography
Authors: Lee Jungyun , Paik Haerin , Hong Yeon Hee , Kim Seul Ki , Lee Jung Ryeol 
Published: January 2024

Background and Aims: Robot-assisted myomectomy is being widely implemented in gynecologic surgery, with strength in cases with multiple myomas. To maximize myoma detection during surgery, intracorporeal ultrasonography (USG) has been introduced. The study aims to evaluate the efficacy of intracorporeal USG in robot-assisted myomectomy. Method: This was a retrospective study of patients who underwent robot-assisted myomectomy from January 2022 to February 2023. The cases with preoperative pelvis magnetic resonance imaging (MRI) and which utilized intracorporeal USG during surgery were identified. Demographic and operative variables were analyzed, along with myoma extraction rates, which were determined as the proportion of surgically removed myomas compared to the number identified in preoperative MRI, and the number of additional myomas removed during the operation. Results: In all patients, the number of myomas removed was greater than the number of myomas in preoperative MRI (12.4 ± 8.6 vs. 7.9 ± 5.0, [Formula: see text]<0.001). An average of 4.5 additional myomas were found, and the extraction rate was 167%. For subgroup analysis, patients were stratified according to preoperative myoma counts: 1-5(n=22), 6-9(n=17), and 10 or more(n=17). For all three groups, more myomas were detected in surgery with the use of intracorporeal USG (Median 4 vs 5, [Formula: see text]=0.001, 7 vs 12, [Formula: see text]<0.001, 12 vs 18, [Formula: see text]=0.005, respectively) and the number of additional myomas removed increased in proportion to the preoperative myoma count. There was a positive correlation between the number of myomas identified in preoperative MRI and the number of additional myomas removed (Pearson’s correlation coefficient: 0.292, p=0.029). Conclusion: Intracorporeal ultrasound improves myoma detection, as significantly more myomas are removed during surgery than anticipated based on preoperative MRI. Moreover, this study demonstrates a positive correlation between the number of myomas identified in preoperative MRI and the additional myomas removed during surgery. Intracorporeal ultrasound may be particularly advantageous for patients with multiple myomas detected on preoperative MRI.
#334 : Tackling Complex Infertility Patient: A Case Report
Authors: Nabila Putri , John Clifford E. , Ekapatria Christofani F.C 
Published: January 2024

Background: Infertility is a common problem presented in women with endometriosis especially as it is often associated with poor quality of life. Therefore, surgical Intervention has been considered as the first-line treatment of choice. On the other hand, surgery itself has detrimental effects on ovarian reserve and is associated with ovarian failure. Case Report: We present a case of a 39-year-old woman para 1 with multiple myoma uteri, bilateral endometriosis cyst, left hydrosalpinx, deep infiltrating endometriosis (DIE), and adhesion (Figure 1). She comes with a history of heavy abnormal uterine bleeding, lower abdominal pain, and unable to conceive 11 years after their first child despite adequate unprotected intercourse. The patient was anemic (Hemoglobin 7.30 g/dL) with low Anti Mullerian Hormone (AMH; 0.26 mg/ml). We decided to perform laparoscopic uterine myoma with multiple bilateral endometrial cysts, left salpingectomy, and adhesiolysis (Figure 2). Patient then underwent an ultra-long protocol with three cycles of leuprolide acetate 3.75 mg. Ovum pick-up was performed and we retrieved five M-II oocytes and from embryo culture, two embryos with good and moderate quality were collected for vitrification procedure. Prior frozen embryo transfer, extra acetylsalicylic acid 80 mg once a day and estradiol valerate 4 mg thrice a day were given for 18 days. The patient soon became pregnant after a few weeks of embryo transfer with βHCG of 185 mIU/m. Conclusion: Individualized treatment plans based on the patient’s symptoms and respective reproductive goals are important points to be discussed in endometriosis-associated infertility patients. Surgical intervention before IVF/ICSI is deemed as necessary to preserve ovarian reserve and to restore normal anatomy to increase the success rate of embryo implantation.
#342 : Asherman’s Syndrome Attributed to Genital Tuberculosis: A Case Report
Authors: Yuad Haviz 
Published: January 2024

Background: Asherman’s syndrome is a rare pathology secondary to intrauterine adhesion formation characterized by menstrual disorders and reproductive dysfunction. Although the cause intrauterine adhesion is thought to be associated with vigorous curettage or previous uterine surgery, other causes such as tuberculosis or schistosomiasis leading to intrauterine adhesion, are particularly important in developing countries. Case Presentation: A 33-year-old, multiparous woman, has applied for care to our hospital after 6 months of secondary amenorrhea. Other than occasional untreated heavy vaginal discharge, she had no past medical history nor prior uterine surgical procedure. No one in her family was treated for tuberculosis. Previously she was given combination oral contraceptive pills for 2 months, with no desirable outcome. On June 2022, we conducted a transvaginal ultrasound, with several features suggestive of uterine synechiae i.e. interrupted endometrial line in sagittal plain, small focal cystic areas within endometrium, and hyperechoic lesion with and without posterior shadowing. We decided to perform a hysteroscopy, with findings of mottled endometrium across the fundus and corpus of the uterus. Adhesiolysis was performed. An intrauterine foley catheter was inserted to prevent the reformation of adhesions. The specimen from this procedure were collected for pathology testing, with positive result for endometrial tuberculosis with caseous necrosis. The patient was treated with anti-tuberculosis drugs for 9 months. Upon completion of her tuberculosis treatment, she received combination oral contraceptive pills for endometrial restoration. She had her first period on May 2022, but only scanty bleeding occurred. On July 2022, she had two days of period with heavier blood flow. She is still closely observed. Conclusion: Asherman’s syndrome attributed to genital tuberculosis may emerge in otherwise healthy individual especially in countries where tuberculosis is prevalent. Although the results of hysteroscopic adhesiolysis in Asherman’s syndrome are encouraging, unfortunately, women with Asherman’s syndrome due to genital tuberculosis have poorer prognosis for restoration of menstrual function as there is usually complete destruction of endometrium by the mycobacterial disease. Well-designed clinical trials are needed to determine the most appropriate diagnostic and therapeutic modalities.
#61 : Frugal Exoscope: A Potential Option for Microsurgery and Training Program in Male Infertility in the Context of Emerging Markets
Authors: Khoa Le Dang , Hoang Le , Thang Le Duc , Trieu Vy Ngo Dinh , Dung Mai Ba Tien , Huy Duong Quang , Nhu Giang Huynh , Handelsmen David 
Published: January 2024

Background and Aims: Conventional microscopes and exoscopes currently play pivotal roles in the microsurgery or training of male infertility. However, they are expensive and unaffordable in low resource settings which limits training or application of advanced methods. The study aimed to introduce a new, low–cost exoscope system and evaluate its frugal innovation. Method: Using animal models, we evaluated a new frugal exoscope system (called DKTA) in experimental microsurgery of vas deferens repair. Five experts in male infertility surgery undertook an anonymous survey to elucidate their experience with DKTA system use. Three crucial criteria (i.e., substantial cost reduction, concentration on core functionalities, optimised performance level) were evaluated to determine whether the DKTA is effective as frugal innovation. The survey used a 5-point Likert scaling of responses to eight statements investigating surgical, ergonomic, and educational aspects. Results: The DKTA system, including an optical microscope and ultrahigh definition camera (4K), was an exoscope placed between 20–40 cm from the surgical field. It was held in position with a camera support arm on casters and connected to a 65-inches smart TV. DKTA system met the three crucial criteria of frugal innovation, including substantial cost reduction, concentration on core functionalities, and optimised performance level. Five male infertility microsurgeons reported positive evaluations of the DKTA system (use modal scores) when performing experimental vasovasostomy. Conclusions: DKTA system, a frugal innovation, has a valuable contribution to the training programme and performance of male infertility microsurgery in low–resource settings. Acknowledgements: We want to send special thanks to the Centre for Training and Scientific Research staff, Tam Anh General Hospital for their logistics support.
#90 : Association of Serum Vitamin D Level with Asthenozoospermic Male
Authors: Ara Rowshan 
Published: January 2024

Background and Aims: Vitamin D deficiency is one of the most common health problems throughout the world and has an effect on reproductive process. But there is conflicting data about potential association between serum vitamin D level and semen quality. Currently available data indicate that vitamin D has a vital role in sperm motility which is crucial for fertility. The aim was to evaluate the association of serum vitamin D level with asthenozoospermic male, a medical condition characterized by reduced sperm motility which can lead to male infertility. Method: This cross-sectional analytic study was conducted on 314 men attending the Department of Reproductive Endocrinology and Infertility, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh. Considering the eligibility criteria all participants were categorized into two groups; Group-I: 157 asthenozoospermic male, Group-II: normozoospermic male, according to WHO ‘strict’ criteria 2010. Blood and semen samples were obtained for assessment, and data were adjusted for age, body mass index (BMI), total motility and progressive motility. Vitamin D levels were classified according to the Endocrine Society Guideline. Statistical analyses were carried out by using Statistical Package for Social Sciences version 22 for Windows. Results: It was observed that mean vitamin D level was 16.63± 5.54 ng/ml in asthenozoospermic group and 19.83± 5.33 ng/ml in normozoospermic group. Mean vitamin D level was significantly (p<0.05) lower in asthenozoospermic group. It was noticed, 86.6% patients were vitamin D deficient (<20 ng/ml) in asthenozoospermic group compared to 56.7% in normozoospermic group. Low vitamin D is 5 times increased risk of developing asthenozoospermia at 95% CI (2.74-8.99). There was a positive significant correlation (r=0.285; p<0.001) between serum vitamin D level with total motility and progressive motility (r=0.232; p<0.001). Conclusion: Study showed a significant association between low serum vitamin D level and Asthenozoospermia. But cross-sectional study makes it difficult to establish causal relationship. Hence, higher quality studies are required.
#97 : Can Platelet Rich Plasma (PRP) Improve Human Ejaculated Sperm Quality? A Systematic Review and Meta Analysis
Authors: Bofe Adelina Amelia Febriani , Dewanto Agung , Widad Shofwal , Chandra Lukman Ade 
Published: January 2024

Background and Aims: Males are found contribute to 50% of infertility cases overall. Abnormal sperm parameters, such as low concentration, low motility, or abnormally morphology is thought to play a major role in male infertility. In many recent studies, Platelet Rich Plasma (PRP) was reported to have potential therapeutic effect. PRP with its rich growth factor composition, has already proven beneficial in regenerative therapy. PRP can improve the structural and functional impairment of the testis, thereby improve the sperm quality as its product. This study aims to identify the improvement of ejaculated sperm concentration, motility, morphology after treatment with PRP. Methods: A systematic search was conducted in PubMed, Cochrane and Google Scholar databases up to November 2022. We used Prisma’s checklist 2020 as a guideline for writing this. To be included in this meta-analysis, eligible studies had to be a clinical trial comparing ejaculated sperm with PRP treatment and no PRP addition. We conducted both quantitative and qualitative analysis of the assessed outcomes. Results: A total of 91 studies were retrieved. After excluding ineligible publication, 5 studies were finally included in the present meta-analysis (n=331). Among them, varied methods of PRP intervention, but all of them had using PRP 2%. By using a random effect model, there is an improvement of the progressive sperm in group with PRP treatment (MD 15,00, 95%-CI 6,71-23,29). Quantitative analysis (meta-analysis) was not carried out for sperm concentration and morphology, but was explained descriptively, that sperm concentration and morphology increased after PRP treatment. The articles included in the analysis have a low risk of bias. Conclusions: There is an improvement in sperm progressivity after treatment with PRP, that also means there is an improvement in human ejaculated sperm quality. Although found beneficial enhancing the sperm progressivity, but still needed strong evidence for further research.