bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of Germline BRCA Mutations in Locally Advanced, Triple Negative Breast Cancer Treated with Platinum Based Neoadjuvant Chemotherapy
Authors: Mutai Raz , Kuchuk Iryna , Goldshtein Alexandra , Yerushalmi Rinat , Rotem Ofer , Lotan Adi Maisel , Bdolah-Abram Tali , Gabizon Alberto , Goldvaser Hadar 
Published: December 2023

Abstract
Background
Whether germline BRCA (gBRCA) mutation affects prognosis of women with triple negative breast cancer (TNBC) and whether it has implications for treatment decisions in the neoadjuvant setting is unclear.
Methods
This is a retrospective two-center cohort study comprising all women with early-stage TNBC who have completed genetic testing and were treated with neoadjuvant dose-dense doxorubicin and cyclophosphamide followed by paclitaxel and carboplatin. All eligible patients treated between 10.2014 and 3.2020 were included. Data on clinico-pathological, pathological response, overall-survival (OS) and disease-free survival (DFS) were evaluated. Differences in clinico-pathological features and outcomes were analyzed according to gBRCA status.
Results
Sixty-four women were included in the final analysis, of which 31 had pathogenic gBRCA mutation and 33 were gBRCA wild-type. Clinico-pathological characteristics were similar between both groups. The odds for pathological complete response (pCR) were significantly higher in gBRCA mutated women (74.2%) compared to BRCA wild-type women (48.5%), p = 0.035. At a median follow-up of 30 months, gBRCA mutated women had significantly favorable OS (HR = 8.64, 95% CI 1.08–69.21, p = 0.042). The difference in DFS did not reach statistical significance (HR = 7.4, 95% CI 0.91–60.27, p = 0.062). The favorable OS for gBRCA mutated women remained significant in multivariate analysis (p = 0.029) and was noted regardless of pathological response (p = 0.018).
Conclusion
Compared to wild-type, gBRCA mutated women with locally advanced TNBC treated with neoadjuvant chemotherapy containing carboplatin had a higher pCR rate and better outcomes. These results strengthen the contention that gBRCA status should be considered when tailoring treatment decisions in women with locally advanced TNBC.
Risk factors of prolonged mechanical ventilation after liver transplantation: a retrospective cohort study
Authors: yu ningna , Wang Huafen , lu fangyan , Shen Shichao , Su Wei 
Published: December 2023

Abstract
Background
The risk factors of prolonged mechanical ventilation (PMV) in patients after liver transplantation remain unclear. The aim was to evaluate the risk factors of PMV to guide clinical nursing programs for early extubation and reduction of PMV complications by a retrospective cohort study.
Methods
505 patients were recruited to conduct a retrospective cohort study by a logistic regression model to determine risk factors for PMV patients after liver transplantation.
Results
129 (25.5%) of 505 patients required PMV. The PMV-associated factors were significantly different between the PMV group and the non-PMV group, which included MELD score, ASA score and TP level. According to logistic regression analysis, MELD score ≥ 18 points, ASA score > 3, TP < 60 g/L, et al., are higher risk for PMV.
Conclusion
The incidence of PMV after liver transplantation is 25.5%. A MELD score ≥ 18 points, ASA rating > 3, TP < 60 g/L, et al, are independent risk factors for PMV.
Osteomyelitis and immune cell phenotypes: a study based on a Mendelian randomisation approach
Authors: Long Kehan , Dong Sumiao , Yu Dou , Gong Ao , Ying Zhendong 
Published: December 2023

Abstract
Background: Osteomyelitis is a severe bone marrow infection, whose pathogenesis is not fully understood. This study aims to explore the causal relationship between immune cell characteristics and osteomyelitis, in hopes of providing new insights for the prevention and treatment of osteomyelitis.
Methods: Based on two independent samples, this study employed a two-sample Mendelian randomization (MR) analysis to assess the causal relationship between 731 immune cell characteristics (divided into 7 groups) and osteomyelitis. Genetic variants were used as proxies for risk factors to ensure the selected instrumental variables meet the three key assumptions of MR analysis. GWAS data for immune characteristics came from the public GWAS catalog, while data for osteomyelitis was sourced from the Finnish database.
Results: At a significance level of 0.05, 21 immune phenotypes were identified as having a causal relationship with the development of osteomyelitis. In the B cell group, phenotypes such as Memory B cell %B cell, CD20- %B cell, and Memory B cell %lymphocyte showed a positive causal relationship with osteomyelitis, while Naive-mature B cell %B cell and IgD- CD38- AC phenotypes showed a negative causal relationship. In addition, specific immune phenotypes in the cDC cell group, Myeloid cell group, TBNK cell group, T cell maturation stage, and Treg cell group also showed significant associations with osteomyelitis. Through reverse MR analysis, it was found that osteomyelitis had no significant causal impact on these immune phenotypes, suggesting that the occurrence of osteomyelitis might not in turn affect these immune cell phenotypes.
Conclusion: This study reveals for the first time the causal relationship between specific immune cell characteristics and the development of osteomyelitis, providing a new perspective for understanding the immune mechanism of osteomyelitis. These findings are significant for formulating targeted prevention and treatment strategies, and hold promise for improving the clinical treatment outcomes of patients’ osteomyelitis.
Infestation and related ecology of chiggers on the large-eared field mouse (Apodemus latronum), an endemic rodent species in southwest China
Authors: Liu Qiao-Yi , Guo Xian-Guo , Fan Rong , Song Wen-Yu , Peng Pei-Ying , Zhao Ya-Fei , Jin Dao-Chao 
Published: December 2023

Abstract
Chiggers generally refer to the larvae of chigger mites, and they are the exclusive vector of scrub typhus. Based on the field surveys in the five provincial regions of southwest China between 2001 and 2022, the present paper first reported the infestation and related ecology of chiggers on the large-eared field mouse (Apodemus latronum), an endemic rodent species in the region. A total of 501 A. latronum were captured and examined, and 933 chiggers collected from the mice were identified as 2 families, 8 genera, and 61 species. The overall infestation prevalence (PM) and mean abundance (MA) of A. latronum with chiggers reached 19.76% and 1.86 mites/host, respectively. Of 61 chigger species identified from the mice, Leptotrombidium bayanense, Neotrombicula tongtianhensis, L. rupestre and L. yongshengense were four dominant species, which were of aggregated distribution among the different individuals of their host, A. latronum. The interspecific relationship analysis showed that a positive or negative correlation existed between any two of the four dominant chigger species. The male A. latronum harbored more chiggers with higher infestation prevalence (PM = 20.25%), mean abundance (MA = 2.31 mites/host) and mean intensity (MI = 11.42 mites/host) than the female mice (PM = 18.68%, MA = 1.11 mites/host, MI = 5.94 mites/host) with p > 0.05. The infestation indices of chiggers on adult A. latronum (PM = 38.28%, MA = 5.11) were higher than those on juvenile mice (PM = 12.63%, MA = 0.97) with p < 0.01, showing an age bias in the infestation. The relative fatness (K) was introduced to reflect the nutrition status of the mouse host. The mouse hosts with good nutrition (K = 3.4±0.89g/cm3) harbored fewer chiggers than the hosts with poor nutrition (K = 2.2±0.90g/cm3) (p < 0.01). Based on Preston’s lognormal model, the theoretical curve of the species abundance distribution of chigger community on A. latronum was successfully fitted, the expected theoretical total number of chigger species on A. latronum in southwest China was estimated to be 93 species.
Unsupervised robot-assisted rehabilitation after stroke: feasibility, effect on therapy dose, and user experience
Authors: Devittori Giada , Dinacci Daria , Romiti Davide , Califfi Antonella , Petrillo Claudio , Rossi Paolo , Ranzani Raffaele , Gassert Roger , Lambercy Olivier 
Published: December 2023

Abstract

Background
Unsupervised robot-assisted rehabilitation is a promising approach to increase the dose of therapy after stroke, which may help promote sensorimotor recovery without requiring significant additional resources and manpower. However, the unsupervised use of robotic technologies is not yet a standard, as rehabilitation robots often show low usability or are considered unsafe to be used by patients independently. In this paper we explore the feasibility of unsupervised therapy with an upper limb rehabilitation robot in a clinical setting, evaluate the effect on the overall therapy dose, and assess user experience during unsupervised use of the robot and its usability.
Methods
Subacute stroke patients underwent a four-week protocol composed of daily 45 minutes-sessions of robot-assisted therapy. The first week consisted of supervised therapy, where a therapist explained how to interact with the device. The second week was minimally supervised, i.e., the therapist was present but intervened only if needed. After this phase, if participants learnt how to use the device, they proceeded to two weeks of fully unsupervised training. Feasibility, dose of robot-assisted therapy achieved during unsupervised use, user experience, and usability of the device were the primary outcome measures. Questionnaires to evaluate usability and user experience were performed after the minimally supervised week and at the end of the study, to evaluate the impact of therapists’ absence.
Results
Unsupervised robot-assisted therapy was found to be feasible, as 12 out of the 13 recruited participants could progress to unsupervised training. During the two weeks of unsupervised therapy participants on average performed an additional 360 minutes of robot-assisted rehabilitation. Participants were satisfied with the device usability (mean System Usability Scale scores > 79), and no adverse events or device deficiencies occurred.
Conclusions
We demonstrated that unsupervised robot-assisted therapy in a clinical setting with an actuated device for the upper limb was feasible and can lead to a meaningful increase in therapy dose.
Trial registration
Registered on 13.05.2020 on clinicaltrials.gov (NCT04388891).
Neutrophil subsets play dual roles in tuberculosis by producing inflammasome dependent-IL-1β or suppressing T-cells via PD-L1
Authors: WINTER Nathalie , DOZ-DEBLAUWE Emilie , BOUNAB Badreddine , CARRERAS Florence , SILVEIRA-FAHEL Julia , OLIVEIRA Sergio C. , LAMKANFI Mohamed , VERN Yves LE , GERMON Pierre , PICHON Julien , KEMPF Florent , PAGET Christophe , REMOT Aude 
Published: December 2023

Abstract
Neutrophils can be beneficial or deleterious during tuberculosis (TB). Based on the expression of MHC-II and programmed death ligand 1 (PD-L1), we distinguished two functionally and transcriptionally distinct neutrophil subsets in the lungs of mice infected with mycobacteria. Inflammatory [MHC-II−, PD-L1lo] neutrophils produced inflammasome-dependent IL-1β in the lungs in response to virulent mycobacteria and “accelerated” deleterious inflammation, which was highly exacerbated in IFN-γR−/− mice. Regulatory [MHC-II+, PD-L1hi] neutrophils “brake” inflammation by suppressing T-cell proliferation and IFN-γ production. Such beneficial regulation, which depends on PD-L1, is controlled by IFN-γR signaling in neutrophils. The hypervirulent HN878 strain from the Beijing genotype curbed PD-L1 expression by regulatory neutrophils, abolishing the braking function and driving deleterious hyper-inflammation in the lungs. These findings add a layer of complexity to the roles played by neutrophils in TB and may explain the reactivation of this disease observed in cancer patients treated with anti-PD-L1.
Dengue fever outbreak in Puntland State, Somalia, November 2022-May 2023
Authors: Jama Saaid Said , Abshir Said Nuriye , Jama Jibril Said , Abdi Mohamed Mohamud 
Published: December 2023

Abstract

Dengue infection is a mosquito-borne, endemic viral disease, particularly in developing countries. Here, we report the results of the clinicodemographic, serologic profile, and monthly occurrence of a recent dengue fever outbreak in Puntland State (Somalia). We analyzed the data of 956 dengue-suspected patients who were investigated using the rapid diagnostic testing (RDTs) method for detecting NS1 (dengue virus non-structural protein) and IgM antibodies employing the SD Biosensor Dengue Dou NS Ag and IgM test kit (Germany) at the Puntland Public Health Referral Laboratory in the period from November 21, 2022, to May 27, 2023. We found that 118 cases were positive for dengue among the suspected patients enrolled in the present study. Of these cases, 86.4% were dengue NSI positive, and 23.7% were dengue IgM positive. The number of females and males in the confirmed cases was equal, and most (48.3%) were aged 20 years or less. 43.1% of them lived in the Nugal region, particularly in Garowe. Clinically, fever was the most frequent symptom (88.9%). The cases peaked in December 2022 but dropped from January to March, with a slight rise in February, and then increased in April and May 2023. We recommend improving vector control measures, strengthening dengue surveillance, developing an early warning system, and future studies to characterize the circulating strains.
Computational Analysis of Gastric Canceromics Data to Identify Putative
Biomarkers
Authors: Saha Sagarika , Vyas Renu 
Published: December 2023

Background::
Gastric cancer develops as a malignant tumor in the mucosa of the stomach,
and spreads through further layers. Early-stage diagnosis of gastric cancer is highly challenging
because the patients either exhibit symptoms similar to stomach infections or show no signs at
all. Biomarkers are active players in the cancer process by acting as indications of aberrant alterations
due to malignancy.

Objective::
Though there have been significant advancements in the biomarkers and therapeutic targets,
there are still insufficient data to fully eradicate the disease in its early phases. Therefore, it
is crucial to identify particular biomarkers for detecting and treating stomach cancer. This review
aims to provide a thorough overview of data analysis in gastric cancer.

Methods::
Text mining, network analysis, machine learning (ML), deep learning (DL), and structural
bioinformatics approaches have been employed in this study.

Results::
We have built a huge interaction network in the current study to forecast new biomarkers
for gastric cancer. The four putatively unique and potential biomarker genes have been identified
via a large association network in this study.

Conclusion::
The molecular basis of the illness is well understood by computational approaches,
which also provide biomarkers for targeted cancer therapy. These putative biomarkers may be useful
in the early detection of disease. This study also shows that in H. pylori infection in early-stage
gastric cancer, the top 10 hub genes constitute an essential component of the epithelial cell signaling
pathways. These genes can further contribute to the future development of effective biomarkers.
Melatonin as a Promising Agent for Cancer Treatment: Insights into
its Effects on the Wnt/beta-catenin Signaling Pathway
Authors: Davoodvandi Amirhossein , Asemi Reza , Sharifi Mehran , Reiter Russel J. , Matini Seyed Amirhassan , Mirhashemi Seyyed Mehdi , Asemi Zatollah 
Published: December 2023

Abstract:
In recent years, substantial advances have been made in cancer treatment modalities.
Yet, within the last three decades, neither cancer incidence nor the cancer-induced
mortality rate has changed. Available anti-cancer chemotherapeutics possess remarkably
restricted effectiveness and often have severe adverse effects. Hence, the identification
of novel pharmaceutical agents that do not exhibit these major disadvantages is
imperative. Melatonin, an important endogenous molecule synthesized and secreted by
the pineal gland, is a promising chemical agent that has been comprehensively assessed
over the last decades for its anti-inflammatory and anti-cancer properties. Melatonin is reportedly
a significant inhibitor of cancer initiation, progression, and metastasis. The anti--
cancer potential of melatonin is principally mediated by reversing the up-regulated
amounts of different transcription factors, growth factors, inflammatory cytokines, protein
kinases, and other oncogenic agents. Also, melatonin often has signifcant inhibitory
effects on cancer cell proliferation through either promoting apoptosis or inducing cell cycle
arrest. The current review provides an insight into melatonin-induced effects against
various human cancers with a particular focus on the regulation of Wnt/β-catenin signaling
pathway.
The impact of COVID-19 on the quality of life among University students with obsessive-compulsive disorder
Authors: Almustafa Reem , Alkhoshi Abdulaziz , Alshaikh Jumana , Ayoub Reema , Aljifri Zainalabden , A. Alfakeh Sulhi 
Published: December 2023

Background:
The coronavirus disease 2019 (COVID-19) pandemic has necessitated precautionary measures to prevent the transmission of the virus, affecting individuals with obsessive-compulsive disorder (OCD). We investigated the impact of the COVID-19 pandemic on the quality of life of university students with OCD.
Methods:
Students with validated confirmed diagnosis of OCD at King Abdulaziz University, Jeddah, received two online surveys: the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS) to approve the diagnosis and the 36-Item Short-Form Health Survey (SF-36) to assess symptom severity and overall quality of life during the pandemic. Students with psychotic disorders were excluded from this study.
Results:
The majority were females (62.5%), with an average age of 23.13 years. The participants had mild to moderate of OCD symptoms. The SF-36 role limitation due to emotional problems domain was significantly associated with the level of OCD symptoms (p=0.023), with higher scores reported for those with mild symptoms. Age was positively correlated with social functioning and pain domains but negatively correlated with Y-BOCS scores. The general health domain was significantly associated with current OCD symptoms and family history of OCD diagnosis.
Conclusions:
The self-reported decline in the severity of OCD symptoms in adult students indicates that the participants in our study with diagnosable OCD perceive an improvement in their symptoms from the previous year.
EL PUERTO DE VALPARAÍSO Y SUS RELACIONES CON LA CIUDAD
Authors: Escobar Luis Eduardo 
Published: December 2023

La historia de los puertos en Chile viene desde la colonización española. De hecho, como los caminos eran de muy mala calidad o simplemente no existían, las rutas marítimas y, por tanto, los puertos, o playas más bien, jugaron un papel central no sólo en el comercio exterior del país sino también en la movilidad de personas y carga dentro del territorio nacional. En este contexto el puerto de Valparaíso ha sido un motor fundamental en el desarrollo económico de la región y del país desde su primer uso por los colonizadores españoles. Este artículo examina la evolución de la actividad portuaria en Valparaíso, como también su impacto en la ciudad desde la década de 1970 hasta nuestros días. Durante este período, el puerto ha enfrentado desafíos significativos y ha experimentado transformaciones sustanciales que le han permitido acomodar buena parte del crecimiento del comercio exterior que se transfiere en la zona central de Chile.
Al cumplir 500 años, en 2026, el Puerto de Valparaíso debería estar bien avanzado hacia la realización de su nueva etapa de expansión con pleno respaldo ciudadano y con una combinación de servicios al comercio exterior, al turismo y a sus habitantes que sigan garantizando que sea, como dice la canción, el Puerto Principal.
Land Use and Land Cover (LULC) Change in Eastern Areas of East Java From 1972 To 2021: Learning From Landsat Image
Authors: Mandala Marga , Indarto Indarto , Mas'udi Ach. Fauzan , Saputra Akhmad Andi 
Published: December 2023

Urban development, population growth, high traffic jams, and intensive disaster events are indicators of changing the landscape of the eastern area of East Java. Investigating these changes is vital for planning and environmental protection in the future. This study examines changes in land use and land cover (LULC) during the past 50 years in the eastern part of East Java from 1972 to 2021. The changes are examined by contrasting four maps derived from Landsat images (1972, 1997, 2013, and 2021). The following are the main study procedures: (1) data inventory, (2) field survey, (3) image processing and classification, and (4) interpretation of LULC changes. With Google Earth Engine, all photos are downloaded (GEE). Landsat image classification was completed using the maximum likelihood algorithm with an overall Kappa accuracy of>85%. Eight (8) major classifications are therefore produced by the classification: (1) the pavement or urban area (PUA); (2) heterogeneous agricultural land (HAL); (3) bare soil (BS); (4) paddy field (PF); (5) open water body (OWB); (6) vegetation/plantation (VG); (7) shrubland (SL); and (8) wetlands (WL). In the areas with rapid development, the LULC change is more pronounced, i.e., Pasuruan, Jember, and Banyuwangi Regencies. LULC change in and near mid-regency and the rural regions comes next. Over the past fifty years, regional growth has resulted in increases in PUA (+4.4 percent), PF (+12.2 percent), and VG (+17.9 percent). On the other hand, the development has decreased SL, BS, and HAL by 5.8 and 15.9 percent, respectively (-13.1 percent ). LULC alterations for human activities have profoundly altered the natural landscape. Keywords: East Java, GEE, Image Processing, Landsat, LULC
Evaluation of an Urban Drainage System in a Big City
Authors: Prayogo Wisnu , Rahmadani Sarra , Wibowo Harry , Fitria Laili , Suryawan I Wayan Koko , Panjaitan Nahesson H. , Luthan Putri Lynna A. , Fitria Novi 
Published: December 2023

Population growth has led to increased runoff and wastewater flowing into drainage channels. This study, therefore, aims to evaluate the drainage capacity of selected channels in Medan City and whether they can still serve the community in the next 10, 20 and 30 years. The observed channel starts from Taduan Street No. 1 to Taduan Street No. 153, with a length of 1,000 m. This evaluation considers population growth, the volume of domestic wastewater, the volume of runoff, the generation of sediment in channels, and evapotranspiration as part of the water cycle. Monthly maximum rainfall data from 2012-2021 from the BMKG Sampali Medan and population data along Taduan are used in the review in this article. Based on the investigation, it is known that the volume of drainage on Taduan Street is no longer able to serve the community even for 2032. It is necessary to review and redesign the dimensions to ensure that wastewater from household activities and runoff does not have a negative impact, especially during the rainy season. With as many as 48 injection wells and the Watershed expansion, it is expected to reduce inundation. Keywords: Drainage channel, Domestic wastewater, Evaluation, Medan, Rainwater