bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of cyclin D2 is associated with increased in vivo invasiveness of human squamous carcinoma cells
Authors: Liu Shao Chen , Bassi Daniel E. , Zhang Shi Yu , Holoran Dana , Conti Claudio J. , Klein‐Szanto Andres J. P. 
Published: October 2002

AbstractOverexpression of cyclin D2 was studied in 10 human squamous cell carcinoma lines, to establish whether this gene plays a role in tumor progression. We found that those cell lines that overexpressed cyclin D2 (CCND2) had the most invasive in vivo behavior. The invasive ability of the cell lines was determined by evaluating the penetration of carcinoma cells into the tracheal wall in an in vivo assay with de‐epithelialized tracheas transplanted into the subcutaneous tissue of nude mice. From five cell lines that exhibited low invasive ability, we selected two that had very little CCND2 expression (SCC9 and SCC15), to evaluate whether CCND2 gene transfer would increase the invasive behavior. After confirming the successful transfer of CCND2 by Northern, Western, and kinase‐activity assays, we assessed the in vivo invasive behavior of the CCND2‐transfected cells and their respective vector alone–transfected controls. The cell lines containing the transferred CCND2 gene had a significantly higher invasive ability than respective controls. This was accompanied by a moderate increase in gelatinase activity. In addition, the in vitro proliferative abilities, under normal culture conditions, of the parental CCND2‐transfected and vector alone–transfected cells were found to be similar, as was the in vivo labeling index of Ki‐67 in the tracheal transplants. These results indicated that the overexpression of CCND2 in squamous cell carcinoma lines modulates cell proliferation after induced quiescence and also has a powerful enhancing effect on in vivo aggressive growth behavior. © 2002 Wiley‐Liss, Inc.
Test–Retest reliability of the Unified Parkinson's Disease Rating Scale in patients with early Parkinson's disease: Results from a multicenter clinical trial
Authors: Siderowf Andrew , McDermott Michael , Kieburtz Karl , Blindauer Karen , Plumb Sandra , Shoulson Ira ,   the Parkinson Study Group
Published: October 2002

AbstractOur objective was to assess the test–retest reliability of the Unified Parkinson's Disease Rating Scale (UPDRS). The UPDRS is the most widely used instrument for measuring severity of parkinsonian symptoms in clinical research and in practice. The validity and inter‐rater reliability of this scale have been previously studied. We examined the test–retest (intrarater) reliability of the UPDRS and derived subscales. Four hundred patients with early‐stage Parkinson's disease (PD) who were participating in a multicenter clinical trial were evaluated using the UPDRS on two separate occasions (screening and baseline visits) prior to receiving treatment. The same neurologist at each center rated the subjects at both examinations that were, on average, 14.6 ± 7.6 days apart (range 3–36 days). Test–retest reliability was estimated using the intraclass correlation coefficient (ICC) for the total UPDRS score, the mental, ADL, and motor subscale scores, and other derived subscale scores. Weighted kappa statistics were calculated for individual UPDRS items. The ICCs for the UPDRS scores were as follows: total score, 0.92; mental, 0.74; ADL, 0.85; motor, 0.90. ICCs for derived symptom‐based scales ranged from 0.69–0.88. Reliability of specific items was generally lower than for summary scales. Reliability was slightly better in patients for whom the testing interval was within 14 days. Based on conventional standards, the UPDRS scores were found to have excellent test–retest reliability in this sample of patients with early PD rated by academic movement disorder specialists. The findings are in agreement with previous reports on interrater reliability. © 2002 Movement Disorder Society.
Post‐Streptococcal autoimmune neuropsychiatric disease presenting as paroxysmal dystonic choreoathetosis
Authors: Dale Russell C. , Church Andrew J. , Surtees Robert A.H. , Thompson Edward J. , Giovannoni Gavin , Neville Brian G.R. 
Published: October 2002

AbstractParoxysmal dystonic choreoathetosis (PDC) is an episodic, non‐kinesogenic, extrapyramidal movement disorder. It is postulated that PDC is an ion channel disorder. We describe a sporadic case of paroxysmal dystonic choreoathetosis occurring after streptococcal pharyngitis. The episodes were characterized by abrupt‐onset dystonic posturing, choreoathetosis, visual hallucinations and behavioral disturbance. Each episode lasted between 10 minutes and 4 hours, and occurred up to 4 times per day. In between attacks, examination was normal. The episodes waxed and waned in frequency during a 6‐month illness. Magnetic resonance imaging of the brain was normal. Post‐streptococcal neuropsychiatric disease has a proposed autoimmune etiology, which is supported by the presence of serum antibasal ganglia antibodies. Western immunoblotting of this case's serum demonstrated antibody binding to a basal ganglia antigens of molecular weight 80 kDa and 95 kDa. Immunohistochemistry examination demonstrated specific antibody binding to large striatal neurones. We propose that autoantibodies produced in post‐streptococcal neuropsychiatric disease cause alteration in neurotransmission, possibly secondary to ion channel binding. © 2002 Movement Disorder Society
Parkinsonian syndrome induced by amlodipine: Case Report
Authors: Teive Hélio A.G. , Germiniani Francisco M.B. , Werneck Lineu César 
Published: October 2002

AbstractAn elderly patient who developed marked symptoms of parkinsonism in response to amlodipine for the treatment of arterial systemic hypertension is described. She had a complete reversal of her motor symptoms after discontinuation of the drug. © 2002 Movement Disorder Society
Effects of apomorphine on flexor reflex and periodic limb movement
Authors: Paradiso Guillermo , Khan Farooq , Chen Robert 
Published: October 2002

AbstractIt has been suggested that periodic leg movements (PLM) and spinal flexor reflex (FR) share common mechanisms. Although dopaminergic agents improve PLM in humans and strongly influence spinal FR circuitry in animal studies, its effects on FR have not been documented in humans. We describe a 65‐year‐old man with PLM after overnight withdrawal of dopaminergic agents. The electromyographic pattern of spontaneous PLM closely resembled that of the FR elicited by medial plantar nerve stimulation. Thirty minutes after subcutaneous injection of apomorphine, both PLM and FR were completely abolished. These findings demonstrate that dopaminergic agents can suppress exaggerated FR in humans, and support the hypothesis of common mechanisms for PLM and FR. © 2002 Movememt Disorder Society.
Novel mobile terminal antenna for meteor burst communication systems
Authors: Fabrés Marta Cabedo , Vilar Juan Escudero , Bataller Miguel Ferrando , Nogueira Alejandro Valero 
Published: October 2002

AbstractThe possibility of establishing a communication channel using meteor burst has been known for decades. In recent years there has been a renewed interest on the subject as a way to offer new wireless services at a low cost. This Letter proposes a novel mobile terminal antenna that fits the stringent size requirements demanded while retaining good gain and polarization purity. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 34: 80–83, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10378
Planar monopole folded into a rectangular‐disk‐like structure as surface‐mountable antenna for 2.4/5.2‐GHz dual‐band operation
Authors: Chang Fa‐Shian , Wong Kin‐Lu 
Published: October 2002

AbstractBy folding a planar monopole made of a copper plate, into a rectangular‐disk‐like structure, a low‐cost surface‐mountable antenna suitable for applications in WLAN (2400–2484 MHz) and HIPERLAN (5150–5350 MHz) systems is proposed. The antenna has compact dimensions of 12 × 8 × 3 mm3, is easy to construct, and can also be easily fed with the use of a 50‐Ω microstrip line. Details of the antenna design and the experimental results for the proposed antenna mounted on a microwave substrate are presented. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 34: 166–169, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10405
Including near‐field constraints for the synthesis of onboard array antennas
Authors: Obelleiro F. , Taboada J. M. , Landesa L. , Castro I. T. , Rodríguez J. L. 
Published: October 2002

AbstractThis article presents a new procedure for the synthesis of array antennas mounted over complex vehicular platforms, where an accurate control of the electromagnetic interference and disturbances (EMI) between systems is required. This is done by including into the overall optimization procedure near‐field restrictions over those regions of the platform where sensitive devices are installed. The electromagnetic coupling between the radiating systems and with the platform is modeled by an accurate three‐dimensional method‐of‐moments formulation. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 34: 188–191, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10413
Numerical analysis of irregular slots in slotted waveguide antennas
Authors: Wang H. Y. , Simkin J. , Sangster A. J. 
Published: October 2002

AbstractA hybrid FEM/MoM method and a finite‐difference–time‐domain (FDTD) simulator have been employed for the modeling of irregular slot radiators in slotted waveguide antennas. Two typical examples of irregular slots are examined, namely, the T‐shaped slot radiator in a bifurcated waveguide and the inclined slot in the narrow wall of rectangular waveguide. In both cases the simulated results are shown to be in good agreement with each other and with measured results. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 34: 342–344, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10457
Conformation techniques for direct‐sequence spread‐spectrum wireless optical systems
Authors: Rabadán J. A. , Bacallado M. A. , Delgado F. , Pérez‐Jiménez R. , López‐Hernández F. J. 
Published: October 2002

AbstractIn this Letter the effect of using pulse conformation techniques over a DSSS wireless optical system is explored. The performances of both Gaussian and sinusoidal conformation over the spread and code signal are compared. As a pattern of comparison, the side‐lobe elimination for adjacent‐channel interference reduction, the process gain, and the BER versus signal to narrow‐band interference ratio have been considered. Simulation results and prototype testing are given so as to illustrate the results obtained with this technique. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 34: 360–364, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10462
A simple closed‐form formula for the mutual impedance of dipoles
Authors: Kazemipour A. , Begaud X. 
Published: October 2002

AbstractA simple and compact formula of dipole mutual impedance is presented. This method gives an analytical expression of mutual impedance between two real, thick center‐fed dipoles in a general geometrical configuration for small distances. The results of this new formulation show a good agreement with the numerical (NEC‐2) and experimental results. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 34: 371–374, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10465
Radiation Q of electrically large loop antennas with nonuniform currents
Authors: Krishnan Sivanand , Li Le‐Wei , Leong Mook‐Seng , Kooi Pang‐Shyan 
Published: October 2002

AbstractAn analysis of the radiation Q of electrically large loop antennas is presented and its characteristics are discussed. The methodology involves the spherical mode expansion for the fields outside the loop. It is found that higher‐order modes need to be considered for a realistic estimate of the radiation Q of large loops. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 34: 377–380, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10467
Stereochemical analysis of the 3α‐ and 3β‐hydroxy metabolites of tibolone through NMR and quantum‐chemical investigations. An experimental test of GIAO calculations
Authors: Colombo Diego , Ferraboschi Patrizia , Ronchetti Fiamma , Toma Lucio 
Published: October 2002

AbstractThe configuration at C‐3 of the 3α‐ and 3β‐hydroxy metabolites of tibolone was studied by extensive application of one‐ and two‐dimensional 1H and 13C NMR spectroscopy combined with molecular modeling performed at the B3LYP/6–31G(d) level. Using HF and DFT GIAO methods, shielding tensors of the two molecules were computed; comparison of the calculated NMR chemical shifts with the experimental values revealed that the density functional methods produced the best results for assigning proton and carbon resonances. Although steroids are relatively large molecules, the present approach appears accurate enough to allow the determination of relative configurations by using calculated 13C resonances; the chemical shift of pairs of geminal α/β hydrogen atoms can also be established by using calculated 1H resonances. Copyright © 2002 John Wiley & Sons, Ltd.
Regulated expression of heparin‐binding EGF‐like growth factor (HB‐EGF) in the human endometrium: A potential paracrine role during implantation
Authors: Lessey Bruce A. , Gui Yaoting , Apparao K.B.C. , Young Steven L. , Mulholland Joy 
Published: October 2002

AbstractHeparin‐binding epidermal growth factor (HB‐EGF) is a recently identified member of the EGF growth factor family found to be expressed in the uterus of both mouse and human at the time of implantation. In the present study, we investigated the expression patterns of HB‐EGF in normal cycling endometrium and compared its expression with the fertility‐associated endometrial epithelial biomarkers αvβ3 integrin, leukemia inhibitory factor (LIF) and homeobox gene, HOXA‐10. RNase protection assay (RPA) using RNA made from endometrium collected from different phases of the menstrual cycle demonstrated increased HB‐EGF expression during the mid‐secretory phase, a pattern similar to, but slightly preceding the expression of αvβ3 integrin and HOXA‐10. In vitro studies demonstrated stimulation of HB‐EGF expression by estradiol‐17β (E2) and progesterone (P4) alone or in combination in stromal cells. Combined treatment with E2 + P4 was, however, required to stimulate epithelial HB‐EGF expression. In vitro experiments demonstrated the ability of HB‐EGF to stimulate epithelial expression of the key endometrial proteins including LIF, HOXA‐10, and the β3 integrin subunit. Each has previously been demonstrated to be an important epithelial biomarker expressed during the implantation window. In addition, conditioned media from endometrial stromal cells treated with E2 + P4 + relaxin mimicked the stimulatory effect of HB‐EGF on epithelial expression of the β3 integrin subunit. The stimulatory effect of the stromal‐conditioned medium was blocked by antibodies that neutralize a known receptor for HB‐EGF. These data suggest that uterine receptivity may be regulated in part by the stromal‐derived HB‐EGF. Mol. Reprod. Dev. 62:446–455, 2002. © 2002 Wiley‐Liss, Inc.
Two novel genes expressed in Xenopus germ line: Characteristic features of putative protein structures, their gene expression profiles and their possible roles in gametogenesis and embryogenesis
Authors: Ikema Yasuko , Hiyoshi Masateru , Daiyasu Hiromi , Toh Hiroyuki , Mori Mitsuaki , Takamune Kazufumi 
Published: October 2002

AbstractWe compared the secondary spermatogonia and the primary spermatocytes of Xenopus for the proteins in their microsomal fractions and identified a newly synthesized protein (94 kDa) and three other proteins (99, 85, and 72 kDa) which increased their amount after entering the meiotic phase. These four proteins were used as antigens to produce polyclonal antibody which was found to react with the four proteins as well as two other proteins (208 and 60 kDa). Immunoscreening of Xenopus testis cDNA library with this polyclonal antibody yielded two cDNA clones (Xmegs and Xtr) encoding novel proteins. Xmegs mRNA was specifically expressed in the spermatogenic cells from the mid‐pachytene stage to completion of two meiotic divisions. The putative Xmegs protein contained 19 tandem repeats of 26 amino acid residues rich in proline as well as potential phosphorylation sites (i.e., serine and threonine residues). Around this repetitive area, we found five PEST sequences known as a proteolytic signal to target protein for degradation. The presence of PEST sequences was believed to allow protein levels to closely parallel mRNA abundance. These results suggested the possible role of this novel protein in the regulation of two meiotic divisions specific to the spermatogenesis in a phosphorylation‐ and/or dephosphorylation‐dependent manner. On the other hand, Xtr mRNA was expressed in both spermatogenic and oogenic cells except for round spermatids and the later stage cells. This mRNA was also expressed in the early stage embryos and its amount was kept constant from the St. I oocyte to the gastrula stage and decreased thereafter. The putative Xtr protein contained four complete and one partial tudor‐like domains that were discovered in Drosophila tudor protein which plays an important role in PGC differentiation and abdominal segmentation. The characteristic expression profile of Xtr and the protein structure similar to the Drosophila tudor protein suggested its possible role in the progression of meiosis and PGC differentiation. Mol. Reprod. Dev. 62:421–430, 2002. © 2002 Wiley‐Liss, Inc.
A model of blood–brain barrier permeability to water: Accounting for blood inflow and longitudinal relaxation effects
Authors: Barbier Emmanuel L. , Lawrence Keith S. St. , Grillon Emmanuelle , Koretsky Alan P. , Décorps Michel 
Published: October 2002

AbstractA noninvasive technique for measuring the permeability of the blood–brain barrier (BBB) to water could help to evaluate changes in the functional integrity of the BBB that occur in different pathologies, such as multiple sclerosis or growth of brain tumor. Recently, Schwarzbauer et al. (Magn Reson Med 1997;37:769–777) proposed an MR method to measure this permeability based on the T1 reductions induced by injecting various doses of paramagnetic contrast agent. However, this method may be difficult to implement in a clinical environment. Described here is a two‐point technique, in which a spatially selective inversion is used to measure T1 prior to and after injection of an intravascular contrast agent. Measurements made in the rat brain are compared to numerical simulations generated with a physiological model that accounts for blood flow and includes two different blood volumes: nonexchanging and exchanging blood volumes. Our results suggest that BBB permeability could be evaluated from the change in T1 caused by the vascular contrast agent. This technique might provide an approach for monitoring changes in BBB permeability to water in clinical studies. Magn Reson Med 47:1100–1109, 2002. © 2002 Wiley‐Liss, Inc.
Neurophysiologic parameters and symptoms in chronic renal failure
Authors: Laaksonen Satu , Metsärinne Kaj , Voipio‐Pulkki Liisa‐Maria , Falck Björn 
Published: October 2002

AbstractWe studied: (1) the sensitivity of various neurophysiologic parameters in the diagnosis of uremic polyneuropathy, (2) the relationship between subjective symptoms and neurophysiologic parameters, and (3) the effect of a single hemodialysis on the neurophysiologic parameters in 21 patients undergoing hemodialysis. The following parameters were studied: sensory and motor nerve conduction, including F‐wave parameters; vibration detection thresholds; and thermal thresholds. The clinical findings and subjective symptoms were studied using a standardized questionnaire. The most sensitive parameters in the diagnosis of uremic neuropathy were F‐wave parameters from lower limbs, vibration detection thresholds from the feet, and the sural nerve sensory action potential amplitude. The nerves from the upper extremities on the side of the fistula should not be used in the diagnosis of uremic polyneuropathy due to numerous mild local nerve lesions. The positive neuropathic symptoms correlated with quantitative vibratory detection thresholds and sensory nerve conduction studies, especially the amplitude of the sensory nerve action potential in the sural nerve. We found no significant change in any of the neurophysiologic parameters following a single hemodialysis session. © 2002 Wiley Periodicals, Inc. Muscle Nerve 25: 000–000, 2002
Activity‐independent neural influences on cat soleus motor unit phenotypes
Authors: Zhong Hui , Roy Roland R. , Hodgson John A. , Talmadge Robert J. , Grossman Elena J. , Edgerton V. Reggie 
Published: October 2002

AbstractThe physiological and phenotypic properties of motor units in the cat soleus muscle were studied after 4 months of inactivity induced by spinal cord isolation (SI). The soleus of some SI cats were stimulated for 30 min/day during an isometric (SI‐I), shortening (SI‐S), or lengthening (SI‐L) phase of a simulated step cycle. Mean maximum tetanic tensions were approximately 15, 26, 32, and 51% of the control in the SI, SI‐S, SI‐L, and SI‐I groups. Mean time‐to‐peak tension was approximately 50% shorter than the control in all SI groups. One motor unit was glycogen‐depleted in each muscle via repetitive stimulation. Eighteen physiologically slow and 9 fast motor units from the spinal cord–isolated groups consisted of fibers that contained only slow myosin heavy chain (MHC) and sarco(endo)plasmic reticulum calcium‐adenotriphosphatase (SERCA) isoforms. Two motor units (physiologically fast) consisted primarily of fibers that contained both fast and slow MHC and SERCA. These data reflect a dissociation between isometric speed‐related properties and MHC and SERCA isoforms following inactivity. The predominance of fibers containing both fast and slow MHC and SERCA isoforms in 2 motor units demonstrates a strong motoneuronal influence on the muscle‐fiber phenotype even when the motoneurons are silent. © 2002 Wiley Periodicals, Inc. Muscle Nerve 26: 252–264, 2002
Class of nerve fiber involvement in sensory neuropathies: Clinical characterization and utility of the plantar nerve action potential
Authors: Nodera Hiroyuki , Logigian Eric L. , Herrmann David N. 
Published: October 2002

AbstractPrecise classification of distal sensory polyneuropathies (SN) according to fiber type involvement is desirable for clinical and research purposes. The sural sensory response has served as an electrophysiologic gold standard for the assessment of large‐fiber sensory dysfunction. However, patients labeled as having small‐fiber sensory neuropathies on the basis of a normal sural response frequently have clinical evidence of large‐fiber dysfunction. The surface‐recorded medial plantar potential has shown promise as a more sensitive indicator of large‐fiber sensory dysfunction, but is not widely accepted because of concerns about age effects and a lack of large well‐controlled studies in SN. We have thus correlated clinical type of SN: large‐fiber sensory neuropathies (LFSN), small‐fiber sensory neuropathies (SFSN), and mixed (large‐ and small‐fiber) sensory neuropathies (MFSN) with sural and medial plantar nerve conduction studies in 133 consecutive patients with distal SN and 108 control subjects. A combination of stringent clinical characterization and electrophysiologic features, especially the surface‐recorded medial plantar rather than sural potential, was complementary, and permitted a more clear separation of LFSN, MFSN, and SFSN than with either approach used alone. © 2002 Wiley Periodicals, Inc. Muscle Nerve 26: 212–217, 2002