bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chlorophyll‐a variation in response to precipitation in a tropical urban lake
Authors: Hamdhani Hamdhani , Sharaha Musyiir , Eppehimer Drew E. , Rizal Samsul 
Published: January 2024

AbstractWith increasing urbanization, urban lakes are becoming more common and serve as important flood control infrastructure, recreational uses, as well as habitat for a variety of biota. However, given their construction, function and location, urban lakes are susceptible to disturbances including stormwater runoff from metropolitan catchment areas. For example, nutrient loading from stormwater runoff can cause eutrophication. Chlorophyll‐a concentration is an important water quality parameter because it is used to assess water quality and determine trophic state. Chlorophyll‐a is known to be impacted by a number of environmental factors including precipitation and associated runoff. Previous studies indicate that the relationship between precipitation and chlorophyll‐a is complex and often site‐specific. In this study, we examined the relationship between chlorophyll‐a and precipitation in an urban, tropical lake located in East Kalimantan Province, Indonesia. From October through December 2022, chlorophyll‐a, turbidity, phosphate (PO4), pH, dissolved oxygen, temperature, total dissolved solids and conductivity were measured at five sampling sites in Air Hitam Lake, and precipitation was measured adjacent to the lake. These data indicated that chlorophyll‐a concentrations were negatively correlated with recent precipitation (r2 = .71, p < .05). This relationship was likely a result of dilution from increased lake volume. Although urban runoff is expected to increase nutrient loading, our monitoring indicated that dilution can mediate this process in the short‐term. Our findings suggest that timing matters when monitoring water quality in tropical, urban lakes. Sampling immediately following heavy precipitation and stormwater runoff can lead to an underestimation of normal chlorophyll‐a concentrations due to dilution. As a result, dilution effects should be examined when studying and managing urban lentic ecosystems with strong precipitation dynamics. Further studies are necessary to better understand these urban aquatic ecosystems to continue to improve future management efforts.
Community‐level post‐hazard functionality methodology for buildings exposed to floods
Authors: Nofal Omar , Rosenheim Nathanael , Kameshwar Sabarethinam , Patil Jayant , Zhou Xiangnan , van de Lindt John W. , Duenas‐Osorio Leonardo , Cha Eun Jeong , Endrami Amin , Sutley Elaina , Cutler Harvey , Lu Tao , Wang Chen , Jeon Hwayoung 
Published: January 2024

AbstractThis paper presents a building‐level post‐hazard functionality model for communities exposed to flood hazards including the interdependencies between the population, buildings, and infrastructure. An existing portfolio of building archetypes is used to model the post‐hazard physical flood functionality of different building typologies within the community with the goal of supporting resilience‐informed decision‐making. Specific fragility‐based flood functionality curves were developed for this portfolio to quantify the exceedance probability of a prescribed set of functionality states. While the physical functionality of buildings is significant to the total functionality of a building and community resilience assessment, the functionality of utilities such as power and water along with the accessibility of the household to essential services such as schools and hospitals is crucial to measure their total functionality. Therefore, functionality models for essential infrastructure were developed to assess housing unit‐ and building‐level functionality following flood hazards. This model also accounts for the functionality of the road network following a flood hazard to identify the level of accessibility of households to different services (e.g., schools, hospitals, gas stations, shopping centers, etc.). The main novelty of this paper is the ability to quantify the total functionality of buildings based on a socio‐physical formulation after including the interdependencies between the functionality of the physical systems and the subsequent functionality of the socio‐economic systems, which is key to measuring resilience at the community level. This was evident from the analysis results using a testbed community of Lumberton, NC, showing that the physical functionality is not sufficient to quantify the total post‐hazard functionality of buildings and that the functionality of other subsystems such as utilities and accessibility to essential services are also needed to quantify the total functionality of buildings and the livability of households.
Endoplasmic reticulum localized TMEM33 domain‐containing protein is crucial for all life cycle stages of the malaria parasite
Authors: Kamil Mohd , Kina Umit Yasar , Atmaca Habibe Nur , Unal Sinem , Deveci Gozde , Burak Pinar , Aly Ahmed S. I. 
Published: January 2024

AbstractEndoplasmic reticulum (ER) plays a pivotal role in the regulation of stress responses in multiple eukaryotic cells. However, little is known about the effector mechanisms that regulate stress responses in ER of the malaria parasite. Herein, we aimed to identify the importance of a transmembrane protein 33 (TMEM33)‐domain‐containing protein in life cycle of the rodent malaria parasite Plasmodium berghei. TMEM33 is an ER membrane‐resident protein that is involved in regulating stress responses in various eukaryotic cells. A C‐terminal tagged TMEM33 was localized in the ER throughout the blood and mosquito stages of development. Targeted deletion of TMEM33 confirmed its importance for asexual blood stages and ookinete development, in addition to its essential role for sporozoite infectivity in the mammalian host. Pilot scale analysis shows that the loss of TMEM33 results in the initiation of ER stress response and induction of autophagy. Our findings conclude an important role of TMEM33 in the development of all life cycle stages of the malaria parasite, which indicates its potential as an antimalarial target.
Investigation of the relationship between spiritual care needs of relatives of patients hospitalized in intensive care unit and intensive care satisfaction in Turkey
Authors: Kaplan Ebubekir , Sir Özkan , Özakgül Aylin 
Published: January 2024

AbstractBackgroundRelatives of patients admitted to the intensive care unit (ICU) play a crucial role in the care of their loved ones. After a patient's admission to the ICU, family members may be vulnerable to conditions such as depression, anxiety or post‐traumatic stress disorder, which can lead to moral concerns.AimThe aim of this study was to determine the relationship between the spiritual care needs of family members of patients hospitalized in the ICU and their satisfaction with the ICU.Study DesignIn this study, a cross‐sectional and correlational design was used. The study was conducted with 291 patient relatives who could be reached by face‐to‐face questionnaire. Data were collected using the ‘Personal Information Form’, ‘Family Satisfaction Scale in Intensive Care Unit (FS‐ICU‐24)’ and ‘Spiritual Care Needs Inventory’.ResultsSpiritual Care Needs Inventory (SCNI) total score was determined as 58.00 ± 15.91. The mean total score of FS‐ICU‐24 was 86.38 ± 7.12. No significant correlation was found between the mean SCNI total score and the FS‐ICU‐24 total score (r = .084; p > .05).ConclusionsAs a result of this research, it was determined that the spiritual care needs of the patients' relatives were at a medium level and their satisfaction with intensive care was high.Relevance to Clinical PracticeInstitutions should establish spiritual care support units for individuals with patients in ICUs and examine the effects of this on individuals.
Anatomical variation of femoral vessels and ultrasound‐guided femoral vein puncture for catheter ablation of arrhythmias
Authors: Guan Wenchi , Li Xiaofeng , Chen Keping , Yao Yan , Liu Jun 
Published: January 2024

AbstractObjectiveThis study aims to investigate the anatomical variations in femoral vasculature and evaluate the clinical value of ultrasound‐guided femoral vein puncture in catheter ablation procedures.MethodsIn this retrospective analysis conducted from January 2023 to March 2023, we examined data from patients who underwent catheter ablation with ultrasound‐guided femoral venipuncture. We evaluated the anatomy of the femoral vasculature at both high and low inguinal levels. Based on the relationship between the femoral vein and artery, we classified the anatomy into four types: Type I (vein parallel to artery without overlap), Type II (vein medial to artery with lumen overlap ≤50%), Type III (vein posterior to artery with lumen overlap > 50%), and Type IV (vein lateral to artery). Additionally, we assessed procedure‐related vascular complications that required interventions or prolonged hospital stays.ResultsA total of 254 patients were included in this study. At the upper inguinal level, most cases (92.5%) exhibited Type II, followed by Type I (6.5%), while Type III (0.6%) and IV (0.4%) were less common. At the lower inguinal level, Type II accounted for 70.7%, there was a significantly higher proportion of Type III (23.4%) and Type IV (5.9%). The overall complication rate was 0.4%, no pseudoaneurysm or hematoma was observed in our study.ConclusionOur study revealed significant anatomical variations in the relationship between the femoral vein and femoral artery. Ultrasound‐guided femoral venipuncture significantly reduced vascular complication rate, making it a valuable tool for guiding puncture procedures.
The signalling pathways, calcineurin B‐like protein 5 (CBL5)‐CBL‐interacting protein kinase 8 (CIPK8)/CIPK24‐salt overly sensitive 1 (SOS1), transduce salt signals in seed germination in Arabidopsis
Authors: Xie Qing , Yin Xiaochang , Wang Yu , Qi Yuting , Pan Chengcai , Sulaymanov Sunnatulla , Qiu Quan‐Sheng , Zhou Yang , Jiang Xingyu 
Published: January 2024

AbstractFor plant growth under salt stress, sensing and transducing salt signals are central to cellular Na+ homoeostasis. The calcineurin B‐like protein (CBL)‐CBL‐interacting protein kinase (CIPK) complexes play critical roles in transducing salt signals in plants. Here, we show that CBL5, an ortholog of CBL4 and CBL10 in Arabidopsis, interacts with and recruits CIPK8/CIPK24 to the plasma membrane. Yeast cells coexpressing CBL5, CIPK8/CIPK24 and SOS1 demonstrated lesser Na+ accumulation and a better growth phenotype than the untransformed or SOS1 transgenic yeast cells under salinity. Overexpression of CBL5 improved the growth of the cipk8 or cipk24 single mutant but not the cipk8 cipk24 double mutant under salt stress, suggesting that CIPK8 and CIPK24 were the downstream targets of CBL5. Interestingly, seed germination in cbl5 was severely inhibited by NaCl, which was recovered by the overexpression of CBL5. Furthermore, CBL5 was mainly expressed in the cotyledons and hypocotyls, which are essential to seed germination. Na+ efflux activity in the hypocotyls of cbl5 was reduced relative to the wild‐type under salt stress, enhancing Na+ accumulation. These findings indicate that CBL5 functions in seed germination and protects seeds and germinating seedlings from salt stress through the CBL5‐CIPK8/CIPK24‐SOS1 pathways.
Enterocolic granulomatous phlebitis associated with epidermal growth factor‐containing fibulin‐like extracellular matrix protein 1 deposition and focal amyloid properties: A case report
Authors: Ichimata Shojiro , Aikawa Akane , Sugishita Naonori , Katoh Nagaaki , Kametani Fuyuki , Tagawa Hibiki , Handa Yusuke , Yazaki Masahide , Sekijima Yoshiki , Ehara Takashi , Nishida Naoki , Ishizawa Shin 
Published: January 2024

AbstractA woman in her 60s with rheumatoid arthritis was admitted with fever and abdominal pain. Laparoscopic examination with the differential diagnosis of peritoneal neoplasm and infection revealed granulomatous phlebitis in the resected greater omentum. Amorphous eosinophilic deposits observed in the resected tissue exhibited focal, weak positivity for Congo red but were strongly positive for thioflavin S, confirming their focal amyloid properties. Marked degeneration of elastic fibers was also evident. Electron microscopy revealed deposits around the affected elastic fibers. Immunohistochemistry revealed the deposition of epidermal growth factor‐containing fibulin‐like extracellular matrix protein 1 (EFEMP1) along with T‐cell‐predominant lymphocytic inflammation. The definitive diagnosis was granulomatous enterocolic lymphocytic phlebitis (ELP) associated with EFEMP1 deposition exhibiting focal amyloid properties (EFEMP1/AEFEMP1), supported by proteomics analysis. This type of vasculitis is similar to amyloid‐β‐related angiitis of the central nervous system. Thus, we speculate that granulomatous ELP also results from an immune response that recognizes EFEMP1/AEFEMP1 deposits as foreign material and attempts to remove them. Confirmation of EFEMP1/AEFEMP1 deposition with Congo red staining is challenging, particularly in the presence of inflammation, and warrants comprehensive evaluation.
Multidimensional preference for technology risk regulation: The role of political beliefs, technology attitudes, and national innovation cultures
Authors: Hemesath Sebastian , Tepe Markus 
Published: January 2024

AbstractBuilding on the concept of participatory regulation, this study emphasizes recognizing the multidimensional character of citizens' risk regulation preferences. Using the case of autonomous vehicles, we specify six technology‐related risks: product safety, regulatory oversight, legal liability, ethical prioritization, data protection, and human supervision. We argue that differences in these multidimensional risk regulation preferences are shaped by citizens' political beliefs, technology attitudes, and national innovation cultures. To test these hypotheses, a conjoint experiment was conducted in the United States (1188 participants), Japan (1135 participants), and Germany (1174 participants) in which respondents compared hypothetical regulation regimes for self‐driving cars, varying alongside the six regulatory risk dimensions. The findings show a universal preference for increased legal responsibility of manufacturers and more stringent safety regulations for autonomous vehicles. Political beliefs and technological attitudes had minimal impact on these preferences. Although there were some cultural differences in privacy and ethical prioritization, no systematic differences were noted across countries, suggesting the possibility of finding common ground in standardizing risk regulations for self‐driving cars.
Changes in running economy and running technique following 6 months of running with and without wearable‐based real‐time feedback
Authors: Van Hooren Bas , Willems Paul , Plasqui Guy , Meijer Kenneth 
Published: January 2024

AbstractBackgroundAn increasing number of commercially available wearables provide real‐time feedback on running biomechanics with the aim to reduce injury risk or improve performance.ObjectiveInvestigate whether real‐time feedback by wearable insoles (ARION) alters running biomechanics and improves running economy more as compared to unsupervised running training. We also explored the correlation between changes in running biomechanics and running economy.MethodsForty recreational runners were randomized to an intervention and control group and performed ~6 months of in‐field training with or without wearable‐based real‐time feedback on running technique and speed. Running economy and running biomechanics were measured in lab conditions without feedback pre and post intervention at four speeds.ResultsTwenty‐two individuals (13 control, 9 intervention) completed both tests. Both groups significantly reduced their energetic cost by an average of −6.1% and −7.7% for the control and intervention groups, respectively. The reduction in energy cost did not significantly differ between groups overall (−0.07 ± 0.14 J∙kg∙m−1, −1.5%, p = 0.63). There were significant changes in spatiotemporal metrics, but their magnitude was minor and did not differ between the groups. There were no significant changes in running kinematics within or between groups. However, alterations in running biomechanics beyond typical session‐to‐session variation were observed during some in‐field sessions for individuals that received real‐time feedback.ConclusionAlterations in running biomechanics as observed during some in‐field sessions for individuals receiving wearable‐based real‐time feedback did not result in significant differences in running economy or running biomechanics when measured in controlled lab conditions without feedback.
Multicultural Training and Program Climate in Master’s Degree Sport Psychology Programs
Authors: Arnold Macey , Petrie Trent 
Published: January 2024

Key governing bodies (e.g., Association for Applied Sport Psychology) recognize that graduate programs must prepare multiculturally competent practitioners. Students and recent graduates of sport psychology master’s degree programs (N = 107, Mage = 26.32, SD = 5.19) reported moderate levels of feeling safe, valued, and comfortable within their program; low to moderate levels of multicultural training integration; and low to moderate satisfaction with their multicultural training. Students of Color (n = 42) compared to White students (n = 63) reported less satisfaction with their multicultural training; felt less safe, comfortable, and valued; and perceived less multicultural integration. Furthermore, perceptions of multicultural training integration and of feeling safe, comfortable, and valued were significantly related to satisfaction with their multicultural training. Training programs are advised to improve integration of multicultural training, invest in program climate to help students feel safe and valued, and ensure continuing education of faculty members.
Exposure to a fungicide for a field-realistic duration does not alter bumble bee fecal microbiota structure
Authors: Hotchkiss Michelle Z. , Forrest Jessica R. K. , Poulain Alexandre J. 
Published: January 2024

ABSTRACT

Social bees are frequently exposed to pesticides when foraging on nectar and pollen. Recent research has shown that pesticide exposure not only impacts social bee host health but can also alter the community structure of social bee gut microbiotas. However, most research on pesticide–bee gut microbiota interactions has been conducted in honey bees; bumble bees, native North American pollinators, have received less attention and, due to differences in their ecology, may be exposed to certain pesticides for shorter durations than honey bees. Here, we examine how exposure to the fungicide chlorothalonil for a short, field-realistic duration alters bumble bee fecal microbiotas (used as a proxy for gut microbiotas) and host performance. We expose small groups of
Bombus impatiens
workers (microcolonies) to field-realistic chlorothalonil concentrations for 5 days, track changes in fecal microbiotas during the exposure period and a recovery period, and compare microcolony offspring production between treatments at the end of the experiment. We also assess the use of fecal microbiotas as a gut microbiota proxy by comparing community structures of fecal and gut microbiotas. We find that chlorothalonil exposure for a short duration does not alter bumble bee fecal microbiota structure or affect microcolony production at any concentration but that fecal and gut microbiotas differ significantly in community structure. Our results show that, at least when exposure durations are brief and unaccompanied by other stressors, bumble bee microbiotas are resilient to fungicide exposure. Additionally, our work highlights the importance of sampling gut microbiotas directly, when possible.

IMPORTANCE
With global pesticide use expected to increase in the coming decades, studies on how pesticides affect the health and performance of animals, including and perhaps especially pollinators, will be crucial to minimize negative environmental impacts of pesticides in agriculture. Here, we find no effect of exposure to chlorothalonil for a short, field-realistic period on bumble bee fecal microbiota community structure or microcolony production regardless of pesticide concentration. Our results can help inform pesticide use practices to minimize negative environmental impacts on the health and fitness of bumble bees, which are key native, commercial pollinators in North America. We also find that concurrently sampled bumble bee fecal and gut microbiotas contain similar microbes but differ from one another in community structure and consequently suggest that using fecal microbiotas as a proxy for gut microbiotas be done cautiously; this result contributes to our understanding of proxy use in gut microbiota research.
Non-purulent myositis caused by direct invasion of skeletal muscle tissue by
Leptospira
in a hamster model
Authors: Miyahara Satoshi , Mori Hiroshi , Fukuda Kazumasa , Ogawa Midori , Saito Mitsumasa 
Published: January 2024

ABSTRACT

Myalgia is a common symptom of
Leptospira
infection in humans. Autopsies have reported that muscle tissue shows degeneration and necrosis of the myofibers and infiltration of inflammatory cells composed mainly of macrophages and lymphocytes. It remains unclear whether
Leptospira
directly infects the muscle and how the infiltrating inflammatory cells are involved in muscle fiber destruction. This study evaluated the relationship between histopathological changes and leptospiral localization in the muscle tissue of a hamster model. The influence of macrophages in skeletal muscle injury was also investigated, using selective depletion of macrophages by administration of liposomal clodronate. Hamsters infected subcutaneously with
Leptospira interrogans
serovar Manilae strain UP-MMC-SM showed myositis of the thighs adjacent to the inoculated area beginning at 6 days post-infection. The myositis was non-purulent and showed sporadic degeneration and necrosis of muscle fibers. The degeneration of myofibers was accompanied by aggregations of macrophages. Immunofluorescence staining revealed leptospires surrounding the damaged muscle fibers. Subcutaneous injection of formalin-killed
Leptospira
or intraperitoneal injection of live
Leptospira
caused no myositis in hamster thighs. Liposomal clodronate treatment in infected hamsters reduced macrophage infiltration in muscle tissue without impacting bacterial clearance. Muscle necrosis was still observed in the infected hamsters treated with liposomal clodronate, and there was no significant change in serum creatine kinase levels compared to those in animals treated with liposomes alone. Our findings suggest that leptospiral invasion of muscle tissue from an inoculation site leads to the destruction of muscle fibers and causes non-purulent myositis, whereas the infiltrating macrophages contribute less to muscle destruction.