bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceived healthiness of sugary drinks and related social norms among adults in five countries: evidence from the International Food Policy Study
Authors: Drolet-Labelle Virginie , White Christine M. , Adams Jean , Kirkpatrick Sharon I. , Jáuregui Alejandra , Pedraza Lilia S. , Provencher Véronique , Sacks Gary , Thrasher James F. , Armendariz Gabriela C. , Barquera Simón , Hammond David , Vanderlee Lana 
Published: January 2025

Abstract

Background
A better understanding of correlates of sugary drink consumption is essential to inform public health interventions. This study examined differences in perceived healthiness of sugary drinks and related social norms between countries, over time, and sociodemographic groups and associations with sugary drink intake.

Methods
This study used annual cross-sectional data from the International Food Policy Study from 2018 to 2021 in Australia, Canada, the United Kingdom, the United States, and Mexico. Analyses examined perceived healthiness of eight beverage types and two types of perceived social norms (descriptive, injunctive) that discourage sugary drink consumption. The 24-item Beverage Frequency Questionnaire was used to estimate beverage intake in the past 7 days. Logistic regression models examined trends over time in odds of perceiving each beverage type as unhealthy and agreeing with social norms discouraging sugary drink consumption, across countries and sociodemographic characteristics. Negative binomial regressions examined associations between perceived healthiness, social norms and consumption.

Results
Energy drinks, regular soft drinks, and diet soft drinks were most frequently perceived as unhealthy in all countries, while water and 100% juice were least frequently perceived as unhealthy. Participants in Mexico had higher odds of perceiving 100% juice, chocolate milk, and iced tea as unhealthy in 2021 compared to 2018 (AOR = 1.71 99%CI 1.10–2.64; AOR = 2.69, 99%CI 1.70–4.26; AOR = 1.79, 99%CI 1.15–2.76; respectively), with little change in other countries. Agreement with social norms discouraging consumption of sugary drinks was higher in Mexico than in other countries. Trends in social norms over time were mostly stable, except in Mexico where participants had higher odds of agreeing with both norms in 2020 compared to 2018 (AOR = 1.27, 99%CI 1.09–1.48 for a descriptive norm and AOR = 1.27 99%CI 1.09–1.49 for an injunctive norm). In most countries, perceiving a beverage as unhealthy and agreeing with social norms discouraging consumption of sugary drink were associated with lower sugary drink consumption, with varying strength of associations across countries and beverage types.

Conclusions
Shifts over time in social norms and perceived healthiness observed in Mexico and associations with intake of sugary drinks in most countries suggest that targeted interventions to change norms and perceptions could help reduce sugary drink consumption.
Detection of early relapse in multiple myeloma patients
Authors: Růžičková Tereza , Vlachová Monika , Pečinka Lukáš , Brychtová Monika , Večeřa Marek , Radová Lenka , Ševčíková Simona , Jarošová Marie , Havel Josef , Pour Luděk , Ševčíková Sabina 
Published: January 2025

Abstract

Background
Multiple myeloma (MM) represents the second most common hematological malignancy characterized by the infiltration of the bone marrow by plasma cells that produce monoclonal immunoglobulin. While the quality and length of life of MM patients have significantly increased, MM remains a hard-to-treat disease; almost all patients relapse. As MM is highly heterogenous, patients relapse at different times. It is currently not possible to predict when relapse will occur; numerous studies investigating the dysregulation of non-coding RNA molecules in cancer suggest that microRNAs could be good markers of relapse.

Results
Using small RNA sequencing, we profiled microRNA expression in peripheral blood in three groups of MM patients who relapsed at different intervals. In total, 24 microRNAs were significantly dysregulated among analyzed subgroups. Independent validation by RT-qPCR confirmed changed levels of miR-598-3p in MM patients with different times to relapse. At the same time, differences in the mass spectra between groups were identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry. All results were analyzed by machine learning.

Conclusion
Mass spectrometry coupled with machine learning shows potential as a reliable, rapid, and cost-effective preliminary screening technique to supplement current diagnostics.
Pregnancy-related chronic type A aortic dissection highlights the importance of thorough prenatal maternal examination
Authors: Șulea Cristina M. , Kiss Anna B. , Ágg Bence , Benke Kálmán , Bartha Elektra , Szilveszter Bálint , Stengl Roland , Csonka Máté , Szabolcs Zoltán , Pólos Miklós 
Published: January 2025

Abstract

Background
Aortic dissection occurs rarely during pregnancy but carries a significantly high vital risk for both the mother and the fetus. Early diagnosis and treatment are critical for a successful outcome.

Case presentation
A 32-year-old pregnant woman at 31 weeks of gestation began experiencing shortness of breath, chest pain, and palpitations, which were attributed to an anxiety disorder she had been previously diagnosed with. The symptoms continued to worsen following the delivery when a chest computed tomography investigation revealed signs of chronic type A aortic dissection and an 80 mm post-dissection aneurysm of the ascending aorta. Aortic repair via composite aortic root replacement surgery (Bentall procedure) and partial aortic arch replacement was performed. The patient’s postoperative evolution was notable for a series of complications.

Conclusions
Our report highlights the importance of thorough maternal examination during pregnancy. The high mortality rates associated with aortic dissection occurring in pregnant women and the possibility of missed intervention due to atypical clinical presentation warrant the need for standardized international protocols aimed at the prevention and timely diagnosis of prenatal aortic disease.
Cell-specific transcriptional signatures of vascular cells in Alzheimer’s disease: perspectives, pathways, and therapeutic directions
Authors: Chaudhuri Soumilee , Cho Minyoung , Stumpff Julia C. , Bice Paula J. , İş Özkan , Ertekin-Taner Nilüfer , Saykin Andrew J. , Nho Kwangsik 
Published: January 2025

Abstract
Alzheimer’s disease (AD) is a debilitating neurodegenerative disease that is marked by profound neurovascular dysfunction and significant cell-specific alterations in the brain vasculature. Recent advances in high throughput single-cell transcriptomics technology have enabled the study of the human brain vasculature at an unprecedented depth. Additionally, the understudied niche of cerebrovascular cells, such as endothelial and mural cells, and their subtypes have been scrutinized for understanding cellular and transcriptional heterogeneity in AD. Here, we provide an overview of rich transcriptional signatures derived from recent single-cell and single-nucleus transcriptomic studies of human brain vascular cells and their implications for targeted therapy for AD. We conducted an in-depth literature search using Medline and Covidence to identify pertinent AD studies that utilized single-cell technologies in human post-mortem brain tissue by focusing on understanding the transcriptional differences in cerebrovascular cell types and subtypes in AD and cognitively normal older adults. We also discuss impaired cellular crosstalk between vascular cells and neuroglial units, as well as astrocytes in AD. Additionally, we contextualize the findings from single-cell studies of distinct endothelial cells, smooth muscle cells, fibroblasts, and pericytes in the human AD brain and highlight pathways for potential therapeutic interventions as a concerted multi-omic effort with spatial transcriptomics technology, neuroimaging, and neuropathology. Overall, we provide a detailed account of the vascular cell-specific transcriptional signatures in AD and their crucial cellular crosstalk with the neuroglial unit.

Graphical Abstract

Endothelial and mural cell types mediate dysregulated transcriptional pathways and cell-cell interactions in AD. The neurovascular unit (NVU) is composed of various cell types, including endothelial cells, mural cells (pericytes, smooth muscle cells), fibroblast neurons, microglia, and astrocytes. Dysregulated transcriptional pathways in AD involve multiple pathways, notably immune responses, and angiogenesis common to both endothelial and mural cells. Additionally, pathways involving neuroinflammation and amyloid clearance are prominent in endothelial cell types, while mural cells exhibit pathways related to growth factors, cytoskeletal remodeling and synaptic function. In addition, crosstalk within the NVU and gliovascular unit (GVU) is altered in AD, with altered cell-cell communication evident, with increased interactions between endothelial cells, pericytes, neurons, and microglia, and decreased interactions between endothelial cells, fibroblasts, astrocytes, and neurons. Figure created with BioRender.com. Abbreviations: AD, Alzheimer's disease; NVU, Neurovascular unit; CNS, Central Nervous System.
Wake-up call for recovery: a paradigm shift to address the deep crisis in Israel’s public mental health services in the shadow of October 7, 2023
Authors: Krivoy Amir , Rosenthal Gadi 
Published: January 2025

Abstract

Background
The events of October 7, 2023, and the subsequent war have starkly exposed the shortcoming of Israel’s public mental health system. This system, already strained by years of underfunding and the COVID-19 pandemic, was unprepared for the surge in mental health needs resulting from these traumatic events. This paper outlines the systemic failures and proposes a comprehensive overhaul reform towards an integrative community-based, recovery-oriented mental health service.

Main body
Israel’s mental health crisis is exacerbated by four converging vectors: a global diagnostic crisis in psychiatry, insufficient biological treatments, chronic underfunding, and a fragmented service model. Diagnostic practices, centered on outdated classifications, fail to address the complexity of severe mental illnesses, resulting in imprecise diagnoses and insufficient treatments. Despite the advent of psychopharmacology, significant advancements in drug efficacy are lacking, with recovery rates stagnating or declining. Financially, mental health in Israel receives only 5.2% of the health budget, far below the 10–16% seen in high-GDP Western countries. The community mental health services reform in 2015 lack effective oversight and incentives, leading to long wait times and inadequate care. Additionally, the fragmentation among funding entities—HMOs, Ministry of Health, and Ministry of Welfare—hampers coordinated care and comprehensive service delivery.

Conclusion
The proposed solution involves shifting from a hospital-biomedical -based to an integrated community-based model, emphasizing recovery over symptom management, based on regional mental health centres as hubs of services. This requires significant investment in community mental health teams, crisis intervention, home treatment, and integrated services. Early intervention, technology utilization, economic incentives for community-based care, and patient and family involvement are crucial components. This transformation aims to create a holistic, efficient, and patient-centered mental health system, better equipped to handle future challenges and reduce the societal and economic burdens of mental illness in Israel.
Impact of commercial gut health interventions on caecal metagenome and broiler performance
Authors: Pangga Gladys Maria , Star-Shirko Banaz , Psifidi Androniki , Xia Dong , Corcionivoschi Nicolae , Kelly Carmel , Hughes Callie , Lavery Ursula , Richmond Anne , Ijaz Umer Zeeshan , Gundogdu Ozan 
Published: January 2025

Abstract

Background
Maintaining gut health is a persistent and unresolved challenge in the poultry industry. Given the critical role of gut health in chicken performance and welfare, there is a pressing need to identify effective gut health intervention (GHI) strategies to ensure optimal outcomes in poultry farming. In this study, across three broiler production cycles, we compared the metagenomes and performance of broilers provided with ionophores (as the control group) against birds subjected to five different GHI combinations involving vaccination, probiotics, prebiotics, essential oils, and reduction of ionophore use.

Results
Using a binning strategy, 84 (≥ 75% completeness, ≤ 5% contamination) metagenome-assembled genomes (MAGs) from 118 caecal samples were recovered and annotated for their metabolic potential. The majority of these (n = 52, 61%) had a differential response across all cohorts and are associated with the performance parameter — European poultry efficiency factor (EPEF). The control group exhibited the highest EPEF, followed closely by the cohort where probiotics are used in conjunction with vaccination. The use of probiotics B, a commercial Bacillus strain-based formulation, was determined to contribute to the superior performance of birds. GHI supplementation generally affected the abundance of microbial enzymes relating to carbohydrate and protein digestion and metabolic pathways relating to energy, nucleotide synthesis, short-chain fatty acid synthesis, and drug-transport systems. These shifts are hypothesised to differentiate performance among groups and cycles, highlighting the beneficial role of several bacteria, including Rikenella microfusus and UBA7160 species.

Conclusions
All GHIs are shown to be effective methods for gut microbial modulation, with varying influences on MAG diversity, composition, and microbial functions. These metagenomic insights greatly enhance our understanding of microbiota-related metabolic pathways, enabling us to devise strategies against enteric pathogens related to poultry products and presenting new opportunities to improve overall poultry performance and health.
Isotope analysis of birds’ eye lens provides early-life information
Authors: Hasegawa Emi A. , Matsubayashi Jun , Tayasu Ichiro , Goto Tatsuhiko , Inoue Haruka , Rossberg Axel G. , Yoshimizu Chikage , Hasegawa Masaru , Akasaka Takumi 
Published: January 2025

Abstract
Early-life environment has a long-lasting effect on later life, though its estimation is often prevented in the wild because of a lack of available methods. Recently, isotope analysis of eye lenses has attracted considerable interest as a means to reconstruct the environmental conditions experienced by animals during the developmental period. This analysis has mostly been confined to fish for practical reasons and remains to be resolved for application to other animals. In this study, we broadened its applicability by developing a novel approach and verifying its usability for the reconstruction of early-life environments. We performed a feeding experiment using Japanese quail (Coturnix japonica), in which we administered two diets: one composed mainly of C3 plants (low δ13C and high δ15N) and the other of C4 plants (high δ13C and low δ15N). Quails in the control group were continuously fed a C3-based diet from hatching until 200 days old, whereas those in the treatment groups (T10, T15, T20, and T40) were switched from the C3 to the C4-based diet at 10, 15, 20, and 40 days after hatching, respectively. We found that the δ13C in the eye lenses of the treatment groups decreased from the center layer to the middle layer of the lens and then increased toward the outer layer, thus reflecting the diet change. In contrast, those of the control group exhibited a decreasing trend and equilibrated at the middle layer of the eye lens, with no increase thereafter. This novel approach revealed the postnatal feeding histories of the diet-shift experiment. The high δ13C values observed in the center of the eye lenses would reflect the prenatal feeding environment, i.e., the C4-based diet consumed by their mothers, which is further reinforced by higher δ15N values at this position due to the consumption of egg yolk-derived nutrition. These results indicate that the avian eye lens can be used as an “isotopic chronicle,” which is a useful tool for reconstructing chronological isotopic information about their early-life history.
When rudeness goes home: the impact of supervisor incivility on employees’ work–family conflict
Authors: Imam Hassan , Bilal Naveed , Sekiguchi Tomoki 
Published: January 2025

Abstract
This study uses the frustration-aggression theory to examine how supervisor incivility relates to employee interpersonal incivility toward customers and co-workers and work–family conflict. Work–family conflict refers to the incompatibility between the demands and responsibilities of an individual’s work role and their family role. Service employees from the banking industry (N = 750) participated in the study’s daily multisource questionnaires over a continuous two-week period. The study’s findings, based on multilevel structural equation modeling, show that supervisors’ hostility toward subordinates increases employee interpersonal deviance toward customers and co-workers, as well as work–family conflict. Additionally, the study found in a parallel mediation that the association between supervisor-initiated incivility and work–family conflict is mediated by employee interpersonal deviance toward co-workers and customers. These findings indicate the detrimental effects of incivility from supervisors on both the workplace and employees’ personal lives. The study suggests that workplace incivility causes a negative spiral of mistreatment where a target of incivility may respond by mistreating other, resulting in a toxic work environment. However, organizations can mitigate the negative effects of incivility and promote productivity and success for employees and organization by investing in employee well-being and creating a respectful work culture.
Natural History of Cytologically Low-Risk Indeterminate Thyroid Nodules
Authors: Teliti Marsida , Chytiris Spyridon , Coperchini Francesca , Cerutti Matteo , Grillini Beatrice , Gallo Maria , Calì Benedetto , Arpa Giovanni , Rotondi Mario , Magri Flavia 
Published: January 2025

Abstract

Context
Thyroid nodules classified cytologically as low-risk indeterminate lesions (TIR3A) on fine-needle aspiration biopsy (FNAB) present a clinical challenge due to their uncertain malignancy risk.

Objective
This single-center study aimed to evaluate the natural history of TIR3A nodules.

Methods
FNABs performed between July 2017 and December 2019 were retrospectively retrieved and patients, with TIR3A nodules were evaluated at baseline and throughout a follow-up based on ultrasound (US) parameters and clinical data.

Results
The final study group encompassed 371 patients with an initial TIR3A cytological result. Among them, 102 were directed to surgery after the first endocrinological evaluation, and 269 were directed to conservative follow-up. Repeat FNAB was performed in 120 out of 269 and 13 further patients underwent surgery following FNAB repetition. The malignancy rate among TIR3A nodules was 16.5%, with most interventions being performed for reasons unrelated to the TIR3A result. Repeat FNAB provided a more definitive diagnosis in 40% of cases, with a 5% increase in malignancy risk. The remaining patients were monitored with clinical and US follow-up. Among all patients with TIR3A cytology managed conservatively (149 without FNAB repetition and 66 with at least one FNAB repetition), no significant changes in nodule size and/or US pattern were observed during a median follow-up of 3.3 years.

Conclusion
These findings suggest that active surveillance is a safe option for managing TIR3A nodules, particularly when no additional risk factors are present. The study highlights the role of repeat FNAB in reducing unnecessary surgeries and underscores the generally indolent nature of TIR3A nodules.
Adrenal Insufficiency Due to Adrenal Insult Following Acute Pancreatitis in a Child
Authors: Sasidharan Pillai Sabitha , Robilliard Renee , Fredette Meghan E , Topor Lisa Swartz , Della Grotta Lynn , Serrano-Gonzalez Monica 
Published: January 2025

Abstract
We report an 8-year-old girl who developed primary adrenal insufficiency (AI) in the setting of severe acute pancreatitis. Symmetrically thickened centrally hypoenhancing adrenal glands were incidentally noted on computed tomography (CT) of the abdomen obtained for evaluation for pancreatitis. Laboratory studies included a low morning cortisol level (1.5 µg/dL [41.4 nmol/L], reference range, 5.5-20 µg/dL [151.7-551.7 nmol/L]) on day of hospitalization (DOH) 5. Cortisol did not rise following administration of 250 µg of cosyntropin. She had elevated adrenocorticotropin (ACTH), low aldosterone and dehydroepiandrosterone sulfate levels, normal very long-chain fatty acid levels, and negative 21-hydroxylase autoantibody. Follow-up CT of the abdomen on DOH 9 demonstrated bilateral adrenal gland enlargement with central hypoenhancement and slightly shaggy appearance at the margins. A diagnosis of AI due to adrenal gland injury in the setting of acute pancreatitis was made. The adrenal injury was attributed to ischemic injury, as there were no findings to suggest substantial hemorrhage. Hydrocortisone and fludrocortisone therapies were initiated. Magnetic resonance imaging of the abdomen obtained 8 months later showed diminutive adrenal glands with a marked decrease in size compared to the initial CT scan finding. She has continued to require replacement doses of hydrocortisone and fludrocortisone.