bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitocytosis Mediated by an Enzyme‐Activable Mitochondrion‐Disturbing Polymer‐Drug Conjugate Enhances Active Penetration in Glioblastoma Therapy
Authors: Xiang Yufan , Wang Bing , Yang Wanchun , Zheng Xiuli , Chen Rongjun , Gong Qiyong , Gu Zhongwei , Liu Yanhui , Luo Kui 
Published: February 2024

AbstractThe application of nanomedicines for glioblastoma (GBM) therapy is hampered by the blood–brain barrier (BBB) and the dense glioblastoma tissue. To achieve efficient BBB crossing and deep GBM penetration, this work demonstrates a strategy of active transcellular transport of a mitochondrion‐disturbing nanomedicine, pGBEMA22‐b‐pSSPPT9 (GBEPPT), in the GBM tissue through mitocytosis. GBEPPT is computer‐aided designed and prepared by self‐assembling a conjugate of an amphiphilic block polymer and a drug podophyllotoxin (PPT). When GBEPPT is delivered to the tumor site, overexpressed γ‐glutamyl transpeptidase (GGT) on the brain–blood endothelial cell, or the GBM cell triggered enzymatic hydrolysis of γ‐glutamylamide on GBEPPT to reverse its negative charge to positive. Positively charged GBEPPT rapidly enter into the cell and target the mitochondria. These GBEPPT disturb the homeostasis of mitochondria, inducing mitocytosis‐mediated extracellular transport of GBEPPT to the neighboring cells via mitosomes. This intracellular‐to‐intercellular delivery cycle allows GBEPPT to penetrate deeply into the GBM parenchyma, and exert sustainable action of PPT released from GBEPPT on the tumor cells along its penetration path at the tumor site, thus improving the anti‐GBM effect. The process of mitocytosis mediated by the mitochondrion‐disturbing nanomedicine may offer great potential in enhancing drug penetration through malignant tissues, especially poorly permeable solid tumors.
Stretchable Substrates with 3D Wave Patterned Surface for Enhanced Mechanical Stability of Indium Tin Oxide Electrodes
Authors: Linnet Jes , Nørgaard Jeppe , Kiil Hans‐Erik , Kjelstrup‐Hansen Jakob 
Published: February 2024

AbstractFlexible electronic devices have promising applications in many future technologies such as wearables, implantables, robotics, and displays. Among the distinct types of mechanical flexibility, stretchability stands as a significant challenge. A particularly demanding objective is the realization of a high‐performance transparent electrode that endures stretching and can be mass produced, all while avoiding additional restrictions on device density. In this work, it is demonstrated that a 3D wave patterned surface provides a threefold improved strain performance of deposited indium tin oxide electrodes, in a statistical comparison of 3D wave patterned and planar surfaces, where indium tin oxide electrodes are stretched to electrical failure. Moreover, this platform alleviates residual thin film stress, allowing for easier handling of the substrates. This study demonstrates the feasibility of attaining stretchability for upcoming electronic devices using a scalable platform that incorporates high‐performance transparent electrode materials using only conventional materials and fabrication steps.
Organo‐Ptii Complexes for Potent Photodynamic Inactivation of Multi‐Drug Resistant Bacteria and the Influence of Configuration
Authors: Chong Hui , Liu Xuanwei , Fang Siyu , Yang Xiaofei , Zhang Yuefei , Wang Tianyi , Liu Lin , Kan Yinshi , Zhao Yueqi , Fan Hongying , Zhang Jingqi , Wang Xiaoyu , Yao Hang , Yang Yi , Gao Yijian , Zhao Qi , Li Shengliang , Plymoth Martin , Xi Juqun , Zhang Yu , Wang Chengyin , Pang Huan 
Published: February 2024

AbstractPtII based organometallic photosensitizers (PSs) have emerged as novel potent photodynamic inactivation (PDI) reagents through their enhanced intersystem crossing (ISC) processes. Currently, few PtII PSs have been investigated as antibacterial materials, with relatively poor performances reported and with structure‐activity relationships not well described. Herein, a pair of configurational isomers are reported of Bis‐BODIPY (4,4‐difluoro‐boradizaindacene) embedded PtII PSs. The cis‐isomer (cis‐BBP) displayed enhanced 1O2 generation and better bacterial membrane anchoring capability as compared to the trans‐isomer (trans‐BBP). The effective PDI concentrations (efficiency > 99.9%) for cis‐BBP in Acinetobacter baumannii (multi‐drug resistant (MDR)) and Staphylococcus aureus are 400 nM (12 J cm−2) and 100 nM (18 J cm−2), respectively; corresponding concentrations and light doses for trans‐BBP in the two bacteria are 2.50 µM (30 J cm−2) and 1.50 µM (18 J cm−2), respectively. The 50% and 90% minimum inhibitory concentration (MIC50 and MIC90) ratio of trans‐BBP to cis‐BBP is 22.22 and 24.02 in A. baumannii (MDR); 21.29 and 22.36 in methicillin resistant S. aureus (MRSA), respectively. Furthermore, cis‐BBP displays superior in vivo antibacterial performance, with acceptable dark and photoinduced cytotoxicity. These results demonstrate cis‐BBP is a robust light‐assisted antibacterial reagent at sub‐micromolecular concentrations. More importantly, configuration of PtII PSs should be an important issue to be considered in further PDI reagents design.
Sustainable sweetpotato production in the United States: Current status, challenges, and opportunities
Authors: George Justin , Reddy Gadi V. P. , Wadl Phillip A. , Rutter William , Culbreath Julianna , Lau Pierre W. , Rashid Tahir , Allan Matthew C. , Johaningsmeier Suzanne D. , Nelson Amanda M. , Wang Ming Li , Gubba Augustine , Ling Kai‐Shu , Meng Yan , Collins Daniel J. , Ponniah Sathish K. , Gowda Prasanna H. 
Published: February 2024

AbstractSweetpotato (Ipomoea batatas L.) is an important staple crop cultivated in over 100 countries, and the storage roots and vines provide food for humans and livestock. Sweetpotato consumption and demand for its value‐added products have increased significantly in the last two decades and have led to new cultivar development, expansion in acreage, and increased demand in the United States and its export markets. Despite the known nutritional components and other health benefits, further research is needed to characterize the genetic diversity and chemical composition related to their storage root qualities, essential in developing consumer‐preferred cultivars that offer host plant resistance against pests and pathogens. There is a critical need for research on non‐pesticidal control approaches that can provide safe, effective, economical, sustainable, and environmentally sound pest and disease management techniques, especially for socially disadvantaged small farmers in the United States. Moreover, climate change can significantly impact future production practices and yield and may directly or indirectly affect crop pests, weeds, and diseases. In this review, we discuss the current status, challenges, and future approaches associated with sweetpotato production practices; health‐promoting properties of sweetpotato cultivars; value‐added products; genetic diversity and germplasm; pest and disease management; weed and water management; pollination ecology; and other agronomic and cultural practices that may impact sustainable sweetpotato production by small‐scale, organic, and large‐scale growers.
Catalytic Synthesis of Carbonyl Compounds Using Acyl Fluorides, Carbamoyl Fluorides, and Fluoroformates: An Overview
Authors: Jung Yujin , Lee Yoonho , Shin Kwangmin 
Published: February 2024

AbstractAcyl fluorides, carbamoyl fluorides and fluoroformates have been employed as efficient reagents in a number of organic syntheses. Their application in catalytic transformations, however, began to be explored in the early 2000s. Recently, these reagents have increasingly gained attention owing to their unique reactivity in diverse catalytic systems. This review aims to overview the advancements in the development of catalytic processes, including transition‐metal catalysis, organocatalysis, and cooperative NHC/photoredox catalysis, where these organofluorine compounds are employed as acyl, carbamoyl, and ester group donors.
Spatiotemporal variations in reef manta ray (Mobula alfredi) residency at a remote meso‐scale habitat and its importance in future spatial planning
Authors: Harris Joanna L. , Hosegood Phil , Embling Clare B. , Williamson Benjamin J. , Stevens Guy M. W. 
Published: February 2024

Abstract
The Chagos Archipelago's vast no‐take marine protected area (MPA, 640,000 km2) provides refuge for elasmobranchs facing unsustainable depletion by fisheries. Nonetheless, illegal, unreported and unregulated (IUU) fishing poses a substantial threat, and potential future changes to the use of the MPA could render elasmobranchs increasingly vulnerable to exploitation, putting geographically isolated populations, such as reef manta rays (Mobula alfredi) at risk of local extinction. Therefore, the species' long‐term movements and habitat use must be identified to help prioritize current enforcement activity and inform future spatial planning.
Passive acoustic telemetry and modelled environmental data were used to investigate variations in 42 tagged M. alfredi utilization of a meso‐scale aggregation hotspot, Egmont Atoll, between 2019 and 2022.
Mobula alfredi displayed the highest levels of residency ever reported (77%), with prolonged absences (>2 months) limited to seven individuals. Egmont atoll was used year‐round, with activity peaks during the southeast monsoon (April – November), particularly at sites on the southwest, while sites on the northwest were predominately frequented in the northwest monsoon (December–March). Tags were most likely to be detected when the Indian Ocean Dipole (IOD) was in a positive phase with a greater mixed layer depth, associated with a depression of chlorophyll α levels in the Indian Ocean. Thus, M. alfredi may be particularly reliant on Egmont Atoll, where they are predominantly observed feeding, when prey resources are limited elsewhere.
In a region where the threat of fisheries is of increasing concern, the identification of crucial M. alfredi habitats is essential for conservation management planning. Given the significant role of Egmont Atoll for the local population, regular IUU enforcement patrols are crucial, particularly during the southeast monsoon. Any future changes to the MPA should prioritize preserving and actively enforcing no‐take regulations at Egmont Atoll.
Postcranial anatomy of Prestosuchus chiniquensis (Archosauria: Loricata) from the Triassic of Brazil
Authors: Mastrantonio Bianca Martins , Lacerda Marcel Baêta , de Farias Brodsky Dantas Macedo , Pretto Flávio Augusto , Rezende Letícia de Oliveira , Desojo Julia Brenda , Schultz Cesar Leandro 
Published: February 2024

AbstractPrestosuchus chiniquensis is the best represented pseudosuchian archosaur from the Pinheiros‐Chiniquá Sequence, Middle‐Late Triassic (Ladinian/Carnian) of the Santa Maria Supersequence, Southern Brazil. Several incomplete specimens attributed to this species have been described, but the morphology of the postcranial skeleton of P. chiniquensis is poorly known. In this contribution we present the postcranial material of the UFRGS‐PV‐0629‐T specimen, concluding its description, as its skull and endocast have already been described. Additionally, histological data provided new information on the poorly known ontogenetic series of P. chiniquensis, and on its growth patterns suggesting a longer period of slow growth when compared to other basal Loricata species. A phylogenetic analysis placed UFRGS‐PV‐0629‐T in a group composed by the lectotype, paralectotype, and other described P. chiniquensis specimens, further corroborating our taxonomic hypothesis, that specimens of basal Loricata collected in Brazil are closely related to each other. Due to the association of characters found in the phylogenetic analysis, the specimen UFRGS‐PV‐0629‐T is attributed as the most complete material ever found for P. chiniquensis. As such, it is clear that the material presented here provides important new information on P. chiniquensis. Based on the results presented here, we revised the diagnosis for P. chiniquensis. However, it also evidences the need for new discoveries and studies of other specimens seeking to understand this and other closely related species, which were important components of worldwide trophic webs of the Triassic biotas.
Application of Trametes pubescens for dye removal and biological pretreatment of sugarcane bagasse
Authors: Abbas Mustansir , Ejaz Uroosa , Sohail Muhammad , Alanazi Abdullah K. 
Published: February 2024

AbstractSugarcane bagasse (SB) is an abundantly available agrowaste that is used extensively as a substrate for the production of different industrial enzymes. It is a renewable resource, rich in fermentable sugars. However, the presence of lignin makes it difficult for microbes to degrade it and so it is difficult to utilize it. Keeping in mind the role of white rot fungi in lignin degradation, Trametes pubescens MB89 was used in this study for the delignification of SB with the simultaneous production of laccase. The fungus was also used for the degradation of malachite green dye. A Box–Behnken design (BBD) was used to optimize variables affecting solid state fermentation of SB for laccase production. The leftover SB after fermentation was used for cellulase production by the thermophilic bacterium Neobacillus sedimentimangrovi UE25. The BBD depicted optimized conditions as 60% moisture, particle size 100 μ, 10 days’ incubation period, 450 μM CuSO4, and a temperature of 30 °C. The laccase titers of 1271 IU mL−1 were obtained under optimized conditions. Neobacillus sedimentimangrovi UE25 produced 4.574 IU mL−1 of endoglucanase and 1.812 IU mL−1 of β‐glucosidase by utilizing fungal pretreated SB. Scanning electron microscopy and Fourier transform infrared spectroscopy confirmed the impact of fungal cultivation on SB. Pores were observed after microbial pretreatment and there were changes in the morphological characteristics such as the pith and fiber of SB. To test the ability of laccase to break down aromatic dyes, we grew the fungus T. pubescens MB89 in a nutrient‐rich medium supplemented with malachite green dye. The fungus was able to remove 73.68% of the malachite green dye from the medium in 24 h. This shows that T. pubescens MB89 has a range of potential applications for environmental and industrial purposes.
Sulforaphane prevents diabetes‐induced hepatic ferroptosis by activating Nrf2 signaling axis
Authors: Savic Nevena , Markelic Milica , Stancic Ana , Velickovic Ksenija , Grigorov Ilijana , Vucetic Milica , Martinovic Vesna , Gudelj Andjelija , Otasevic Vesna 
Published: February 2024

AbstractRecently, we characterized the ferroptotic phenotype in the liver of diabetic mice and revealed nuclear factor (erythroid‐derived‐2)‐related factor 2 (Nrf2) inactivation as an integral part of hepatic injury. Here, we aim to investigate whether sulforaphane, an Nrf2 activator and antioxidant, prevents diabetes‐induced hepatic ferroptosis and the mechanisms involved. Male C57BL/6 mice were divided into four groups: control (vehicle‐treated), diabetic (streptozotocin‐induced; 40 mg/kg, from Days 1 to 5), diabetic sulforaphane‐treated (2.5 mg/kg from Days 1 to 42) and non‐diabetic sulforaphane‐treated group (2.5 mg/kg from Days 1 to 42). Results showed that diabetes‐induced inactivation of Nrf2 and decreased expression of its downstream antiferroptotic molecules critical for antioxidative defense (catalase, superoxide dismutases, thioredoxin reductase), iron metabolism (ferritin heavy chain (FTH1), ferroportin 1), glutathione (GSH) synthesis (cystine‐glutamate antiporter system, cystathionase, glutamate‐cysteine ligase catalitic subunit, glutamate‐cysteine ligase modifier subunit, glutathione synthetase), and GSH recycling ‐ glutathione reductase (GR) were reversed/increased by sulforaphane treatment. In addition, we found that the ferroptotic phenotype in diabetic liver is associated with increased ferritinophagy and decreased FTH1 immunopositivity. The antiferroptotic effect of sulforaphane was further evidenced through the increased level of GSH, decreased accumulation of labile iron and lipid peroxides (4‐hydroxy‐2‐nonenal, lipofuscin), decreased ferritinophagy and liver damage (decreased fibrosis, alanine aminotransferase, and aspartate aminotransferase). Finally, diabetes‐induced increase in serum glucose and triglyceride level was significantly reduced by sulforaphane. Regardless of the fact that this study is limited by the use of one model of experimentally induced diabetes, the results obtained demonstrate for the first time that sulforaphane prevents diabetes‐induced hepatic ferroptosis in vivo through the activation of Nrf2 signaling pathways. This nominates sulforaphane as a promising phytopharmaceutical for the prevention/alleviation of ferroptosis in diabetes‐related pathologies.
Plateletcrit is predictive of clinical outcome and prognosis for early‐stage breast cancer: A retrospective cohort study based on propensity score matching
Authors: Zhao Xu , Yang Yilan , Pan Zhe , Lv Weiluo , Rao Xinxin , Wang Xuanyi , Yu Xiaoli 
Published: February 2024

AbstractPurposeBreast cancer (BC) is diagnosed as the most common cancer in women in 2022 according to the American Cancer Society. It is essential to detect early and treat early. Several studies have shown that some blood parameters have important predictive value for BC. In this study, we aim to explore whether some immune‐associated blood parameters are relevant to disease‐free survival (DFS) in early‐stage BC.MethodsA single‐center, regression cohort study of 1490 patients with early‐stage BC in Shanghai Cancer Center was conducted from January 2008 to December 2016. The patients were matched according to the ratio of 1:1 based on Propensity Score Matching (PSM). All patients who experienced disease progression were matched successfully. Thus, 58 pairs of subjects were obtained. Matched blood parameters were evaluated by paired samples t‐test or Wilcoxon signed‐rank test. Factors with statistical difference were further evaluated by stratified COX regression model.ResultsUnivariate analysis showed differences in platelet‐related parameters (PLT, PCT, and PLR) and NLR between the two matched groups. However, stratified COX regression analysis, which ruled out the confounding effects of multiple factors, found that only PCT had prognostic value in early BC patients at baseline and study endpoint. Meanwhile, platelet‐related parameters (PLT, MPV) and NLR were different in the progressive group by self before and after comparison. However, the multiple‐factor analysis showed that only the NLR had prognostic value. ROC curve analysis indicated that the best sensitivity (65.45%) and specificity (78.18%) were obtained when the baseline PCT was 0.225. The optimal sensitivity (70.91%) and specificity (65.45%) were obtained when the PCT of disease progression was 0.215. The Kaplan–Meier curve was used to calculate the DFS rate based on the critical values of the two groups.ConclusionsSome blood parameters have value to predict DFS in early‐stage BC patients, especially platelet‐associated parameters.
Somatic mutations in a multigene panel and impact on prognosis based on TP53 status in Chinese HER2‐positive patients undergoing neoadjuvant therapy: A single‐institution retrospective cohort
Authors: Xiong Min , Wang Xuliren , Liu Douwaner , Xiu Bingqiu , Zhang Qi , Chi Weiru , Goh Chih Wan , Zhang Liyi , Chen Ming , Ren Hengyu , Shao Zhi‐Ming , Yang Benlong , Wu Jiong 
Published: February 2024

AbstractBackgroundGene mutations play a crucial role in the occurrence and development of tumors, particularly in breast cancer (BC). Neoadjuvant therapy (NAT) has shown greater clinical benefit in HER2‐positive breast cancer. However, further clinical investigation is needed to fully understand the correlation between genetic mutations and NAT efficacy and the long‐term prognosis in HER2‐positive BC.MethodsThis was a retrospective cohort study of 222 patients receiving NAT between 2017 and 2021 in the Department of Breast Surgery of Fudan University Shanghai Cancer Center. Tumor samples from these patients were subjected to Next Generation Sequencing (NGS) to analyze mutations in 513 cancer‐related genes. This study aimed to investigate the association between these genetic mutations and postoperative pathological complete response (pCR), as well as their impact on disease‐free survival (DFS).ResultsIn total, 48.65% patients reached pCR, ER‐negative status (p < 0.001), PR‐negative status (p < 0.001), Ki67 ≥ 20 (p = 0.011), and dual‐targeted therapy (p < 0.001) were all associated with enhanced pCR rates. The frequency of somatic alterations in TP53 (60%), PIK3CA (15%), and ERBB2 (11%) was highest. In the HER2+/HR‐ cohort, patients who achieved pCR had a significant benefit in prognosis (HR = 3.049, p = 0.0498). KMT2C (p = 0.036) and TP53 (p = 0.037) mutations were significantly increased in patients with DFS events. Moreover, TP53 mutations had prognostic significance in HER2‐positive BC patients with HR‐negative (HR = 3.712, p = 0.027) and pCR (HR = 6.253, p = 0.027) status and who received herceptin‐only targeted therapy (HR = 4.145, p = 0.011).ConclusionsThe genetic mutation profiles of Chinese HER2+ patients who received NAT were discrepant with respect to HR status or DFS events. TP53 mutations have significant prognostic value in patients with NAT for HER2‐positive BC and patients benefit differently depending on HR status, the neoadjuvant regimen and response, which highlights the significance of genetic factors in treatment customization based on individual genetic and clinical characteristics.
Efficacy of first‐line treatment options beyond RET‐TKIs in advanced RET‐rearranged non‐small cell lung cancer: A multi‐center real‐world study
Authors: Ge Yihui , Li Juan , Gong Wenjing , Wang Jian , Wei Xiaojuan , Liu Jing , Wang Shuyun , Wang Leirong , Sun Haifeng , Cheng Qinglei , Sun Yanxin , Dang Qi , Sun Yuping , Gao Aiqin 
Published: February 2024

AbstractBackgroundAlthough RET‐tyrosine kinase inhibitors (RET‐TKIs) are the preferred first‐line therapy for advanced RET‐arranged NSCLC, most patients cannot afford them. In this population, bevacizumab, immunotherapy, and chemotherapy are the most commonly used regimens. However, the optimal scheme beyond RET‐TKIs has not been defined in the first‐line setting.MethodsThis retrospective study included 86 stage IV NSCLC patients harboring RET rearrangement from six cancer centers between May 2017 and October 2022. RET‐TKIs, chemotherapy, or one of the combination therapies (including immune checkpoint inhibitor (ICI) combined with chemotherapy (I + C), bevacizumab combined with chemotherapy (B + C), ICI and bevacizumab combined with chemotherapy (I + B + C)), were used as the first‐line therapeutics. The clinical outcomes and safety were evaluated.ResultsFourteen of the 86 patients received RET‐TKIs, 57 received combination therapies, and 15 received chemotherapy alone. Their medium PFS (mPFS) were 16.92 months (95% CI: 5.9–27.9 months), 8.7 months (95% CI: 6.5–11.0 months), and 5.55 months (95% CI: 2.4–8.7 months) respectively. Among all the combination schemes, B + C (p = 0.007) or I + B + C (p = 0.025) gave beneficial PFS compared with chemotherapy, while I + C treatment (p = 0.169) generated comparable PFS with chemotherapy. In addition, I + B + C treatment had a numerically longer mPFS (12.21 months) compared with B + C (8.74 months) or I + C (7.89 months) schemes. In terms of safety, I + B + C treatment led to the highest frequency of hematological toxicity (50%) and vomiting (75%), but no ≥G3 adverse effect was observed.ConclusionsI + B + C might be a preferred option beyond RET‐TKIs in the first‐line therapy of RET‐arranged NSCLC. Combination with Bevacizumab rather than with ICIs offered favorable survival compared with chemotherapy alone.