bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real‐world study of lymphadenectomy in patients with advanced epithelial ovarian cancer
Authors: Yin Ziran , Wang Ming 
Published: February 2024

AbstractBackgroundThe evidence on the role of retroperitoneal lymphadenectomy is limited to less common histology subtypes of epithelial advanced ovarian cancer.MethodsThis retrospective cohort study utilized data from the Surveillance, Epidemiology, and End Results Program from January 1, 2010, to December 31, 2019. Patients with stage III–IV epithelial ovarian cancer were included and divided into two groups based on whether they received retroperitoneal lymphadenectomy. The primary outcomes are overall survival (OS) and cause‐specific survival (CSS).ResultsAmong the 10 184 included patients, 5472 patients underwent debulking surgery with retroperitoneal lymphadenectomy, while 4712 patients only underwent debulking surgery. No differences were found in the baseline information between the two groups after propensity score matching. Retroperitoneal lymphadenectomy during debulking surgery was associated with improved 5‐year OS (43.41% vs. 37.49%, p < 0.001) and 5‐year CSS (46.43% vs. 41.79%, p < 0.001). Subgroup analysis further validate the retroperitoneal lymphadenectomy increased the 5‐year OS and CSS in patients with high‐grade serous cancer. Although the results were not validated in the less common ovarian cancer (including endometrial cancer, mucinous cancer, low‐grade serous cancer, and clear cell cancer), the tendency showed patients with the above four subtypes may benefit from the lymphadenectomy which is restricted for small sample size after propensity score matching.ConclusionsThis study revealed that retroperitoneal lymphadenectomy could further improve the survival outcome during debulking surgery in patients with advanced epithelial ovarian cancer. The conclusion was affected by the histology subtypes of ovarian cancer and further studies are needed to validate the conclusion in less common ovarian cancer.
Sensorineural deafness in purebred white Devon Rex cats
Authors: Kortas Annemarie , Pomianowski Andrzej , Kolecka Malgorzata , Rytel Liliana 
Published: February 2024

AbstractBackgroundData regarding congenital sensorineural deafness (CSD) in client‐owned, white Devon Rex cats is limited because most of the information on this disease comes from experiments on mixed‐breed cats.ObjectivesProvide data on the occurrence of CSD in a population of client‐owned purebred white Devon Rex cats.AnimalsForty client‐owned, purebred, white Devon Rex cats examined at 2 different facilities. Median age of the examined cats was 19 weeks.MethodsHearing status was defined by use of brainstem auditory evoked responses.ResultsThe occurrence of sensorineural deafness in the studied population of Devon Rex cats was estimated at 10%. Unilateral and bilateral deafness occurred equally often, with 2 individuals having each (ie, 5.0%). No association between the occurrence of CSD and sex could be found, χ2 (1, n = 40) = 0.001 (P > .99). No association between blue irises and deafness was noted in the studied population, χ2 (1, n = 40) < 0.01 (P > .99).ConclusionsThe occurrence of CSD in a population of client‐owned, white Devon Rex cats was found to be lower compared with data obtained in previously conducted studies of deafness in purebred cats. In the studied population of Devon Rex cats, no association between blue irises and CSD was found.
Assessment of mercury concentrations in fur, liver, and brain tissue from necropsied southern sea otters (Enhydra lutris nereis)
Authors: Hawk Draven W. , Dunkin Robin C. , Batac Francesca , Miller Melissa , Weiss‐Penzias Peter 
Published: February 2024

AbstractSouthern sea otters (Enhydra lutris nereis) are a federally listed threatened keystone species of the California kelp forest ecosystem and a sentinel species, indicating environmental health. Mercury (Hg) is a bioaccumulative neurotoxin that poses a threat to high trophic‐level species such as marine mammals. We quantified total Hg (THg) concentrations in fur, brain, and liver tissues of necropsied southern sea otters (n = 44) that stranded along the California coast from 2010 to 2019. THg concentrations differed significantly between fur and brain, and between liver and brain. Tissue type explained 64%, and sex explained only 3.3% of overall THg variability (0.48–46.1 μg/g dry weight). All individuals had liver Hg concentrations measuring well under the proposed lower‐limit low risk guideline for harp seals, Pagophilus groenlandicus. However, six sea otters exceeded the liver THg moderate risk threshold for terrestrial furbearers to which sea otters are closely related. These data represent baseline THg concentrations in fur, brain, and liver of the southern sea otter and build upon previous studies in various sea otter subspecies. Research on sea otter Hg tolerance and continued monitoring are needed to clarify potential health risks associated with the tissue concentrations observed in this study.
Profiling of deubiquitinases that control virulence in the pathogenic plant fungus Fusarium graminearum
Authors: Chen Ahai , Han Xingmin , Liu Chao , Zhou Yifan , Ren Yiyi , Shen Xingxing , Shim Won Bo , Chai Yunrong , Ma Zhonghua , Chen Yun 
Published: February 2024

Summary
Eukaryotes have evolved sophisticated post‐translational modifications to regulate protein function and numerous biological processes, including ubiquitination controlled by the coordinated action of ubiquitin‐conjugating enzymes and deubiquitinating enzymes (Dubs). However, the function of deubiquitination in pathogenic fungi is largely unknown.
Here, the distribution of Dubs in the fungal kingdom was surveyed and their functions were systematically characterized using the phytopathogen Fusarium graminearum as the model species, which causes devastating diseases of all cereal species world‐wide.
Our findings demonstrate that Dubs are critical for fungal development and virulence, especially the ubiquitin‐specific protease 15 (Ubp15). Global ubiquitome analysis and subsequent experiments identified three important substrates of Ubp15, including the autophagy‐related protein Atg8, the mitogen‐activated protein kinase Gpmk1, and the mycotoxin deoxynivalenol (DON) biosynthetic protein Tri4. Ubp15 regulates the deubiquitination of the Atg8, thereby impacting its subcellular localization and the autophagy process. Moreover, Ubp15 also modulates the deubiquitination of Gpmk1 and Tri4. This modulation subsequently influences their protein stabilities and further affects the formation of penetration structures and the biosynthetic process of DON, respectively.
Collectively, our findings reveal a previously unknown regulatory pathway of a deubiquitinating enzyme for fungal virulence and highlight the potential of Ubp15 as a target for combating fungal diseases.
Brown planthopper infestation on rice reduces plant susceptibility to Meloidogyne graminicola by reducing root sugar allocation
Authors: Xiao Liying , Gheysen Godelieve , Yang Mingwei , Xiao Xueqiong , Xu Lihe , Guo Xiaoli , Yang Lijie , Liu Wen , He Yueping , Peng Deliang , Peng Huan , Ma Kangsheng , Long Haibo , Wang Gaofeng , Xiao Yannong 
Published: February 2024

Summary
Plants are simultaneously attacked by different pests that rely on sugars uptake from plants. An understanding of the role of plant sugar allocation in these multipartite interactions is limited.
Here, we characterized the expression patterns of sucrose transporter genes and evaluated the impact of targeted transporter gene mutants and brown planthopper (BPH) phloem‐feeding and oviposition on root sugar allocation and BPH‐reduced rice susceptibility to Meloidogyne graminicola.
We found that the sugar transporter genes OsSUT1 and OsSUT2 are induced at BPH oviposition sites. OsSUT2 mutants showed a higher resistance to gravid BPH than to nymph BPH, and this was correlated with callose deposition, as reflected in a different effect on M. graminicola infection. BPH phloem‐feeding caused inhibition of callose deposition that was counteracted by BPH oviposition. Meanwhile, this pivotal role of sugar allocation in BPH‐reduced rice susceptibility to M. graminicola was validated on rice cultivar RHT harbouring BPH resistance genes Bph3 and Bph17.
In conclusion, we demonstrated that rice susceptibility to M. graminicola is regulated by BPH phloem‐feeding and oviposition on rice through differences in plant sugar allocation.
Untargeted metabolomic in basil cell cultures – a case study of Ocimum basilicum L. var. minimum Alef.
Authors: Jakovljević Dragana , Kruszka Dariusz , Waligórski Piotr , Warchoł Marzena , Skrzypek Edyta 
Published: February 2024

AbstractAimsDue to the lack of experimental databases, together with the chemical complexity and the dynamic nature of plants' metabolome, most of the metabolites in complex biological materials (like plant in vitro tissue cultures) are not‐annotated, unidentified metabolites. In this study, a method for further metabolite characterization and classification based on the UPLC‐HESI‐HRMS/MS approach for small‐leaved basil (Ocimum basilicum L. var. minimum Alef.) callus and cell suspension culture is presented.Key resultsA total of 2168 metabolic features were detected, out of which the database for exact mass metabolic profiling for 1949 metabolites is presented here since there is no available database dedicated to O. basilicum. We further focused on secondary metabolites (particularly phenolic compounds). The presence of 60 different phenolic compounds belonging mainly to the groups of flavonoids, glycosides, terpenoids, and phenolic acids is confirmed. By comparing relative abundances of phenolic compounds from callus culture and cell suspension culture, both grown on two types of media, via svd‐PCА, univariate analysis, post‐hoc tests, and heatmapping of metabolites, we provided a practical example of how resources presented here can be further applied in tissue culture‐based basil metabolomics studies.UtilityThis study represents the first approach toward routine targeted investigation of secondary metabolites in basil in vitro cultures and provides various opportunities for new‐generation analyses.
Microscale Surface Defects Influence on Thermally Sprayed Alumina Droplets Deformation and Splashing Dynamics
Authors: Nastic Aleksandra , Pershin Larry , Mostaghimi Javad 
Published: February 2024

Abstract
During plasma spraying, interaction between splats and surface microsized features can be critical to the splat dynamic progress and consequently to the coating microstructural development and interfacial bonding. The transient spreading of molten alumina impacting a flat substrate exhibiting micro-obstructions, commonly produced during surface machining, grinding and/or even polishing, is numerically investigated using a three-dimensional model comprising of splat solidification and shrinkage developments. Single isolated splats are also experimentally characterized using top surface scanning electron microscope analysis. Droplets impacting directly onto a microsized surface protuberance show no signs of premature splashing behavior. The microscopic features (&lt;2.5 μm) are not able to generate flow instabilities to initially affect the splat inherent overall spreading. However, subsequent splat peripheral contact with target surface micro-obstructions, characterized by peak and valley features, induces peripheral lift, waviness, and instability. It follows that the ejected destabilized material shears/fractures during stretching triggering the formation of splash fingers. Solidification plays a major role in detracting the role of surface micro-obstructions, i.e., surface roughness, in splashing phenomena.
Thermal Characterization of Subcooled Flow Boiling in a Pin-Fin Coldplate With Non-Uniform Heating
Authors: Osman Ammar , Joshi Yogendra 
Published: February 2024

Abstract
Coldplates are a crucial component in various cooling applications, such as cooling data center servers and power electronics. The unprecedented growth in electronics power density, along with the resulting ultrahigh heat fluxes, demands a transition from single-phase forced convection to two-phase flow boiling heat transfer. The majority of studies in the literature have focused on flow boiling in fin-enhanced silicon microgaps and microchannels, with only a few addressing flow boiling in millimeter-scale heat sinks. In the present study, flow boiling of HFE-7200 dielectric fluid in a millimeter-scale pin-fin coldplate is experimentally investigated under nonuniform heating conditions. Four background heaters represent the low-dissipating-power devices. On the other hand, five hotspot heaters mimic the high-heat-flux devices and generate heat fluxes ranging from 50 W/cm2 to 1000 W/cm2, corresponding to hotspot heat inputs ranging from 62.5 W to 1.25 kW, respectively. The coldplate's thermohydraulic performance is investigated for various flow rates and inlet temperature ranging from 0.5 L/min to 1.5 L/min and from 25 °C to 60 °C, respectively. A high-speed camera is utilized for a narrow field of view (FOV) flow visualization at a frame rate of 2229 fps while a digital camera is used for a wider FOV at 60 fps. Flow visualization demonstrated the transition between bubbly, slug/churn, and stratified two-phase flow regimes.
High Power Density Thermal Energy Storage With Phase Change Material in Enhanced Compact Heat Exchangers
Authors: Kannan Sarath , Jog Milind A. , Manglik Raj M. 
Published: February 2024

Abstract
Performance of a novel ultracompact thermal energy storage (TES) heat exchanger, designed as a microchannel finned-tube exchanger is presented. With water as the heating–cooling fluid in the microchannels, a salt hydrate phase change material (PCM), lithium nitrate trihydrate (LiNO3 · 3H2O), was encased on the fin side. To establish the hypothesis that small-length-scale encasement (&lt;3 mm) of PCM substantially enhances heat transfer to yield very high power-density energy storage, heat exchanger designs with 10 and 24 fins/inch were considered. They were subjected to thermal cycling, or repeated heating (melting) and cooling (freezing), with inlet fluid flow mimicking diurnal variation between 42 °C and 25 °C (representing typical arid-region conditions) over an accelerated time period. By employing salt self-seeding to obviate subcooling during cooling or recrystallization, the TES was found to exhibit stable long-term (100 heating–cooling cycles) operation with very high PCM-side heat transfer coefficients (∼100–500 W/m2 K) and storage power density (∼160–175 kW/m3). In fact, with optimization of heating–cooling fluid flowrate for given charging–discharging time period and exchanger size, power density &gt;300 kW/m3 can be achieved. The results clearly establish that highly compact heat exchangers used as TES units can provide very high-performance alternatives to conventional ones.
Design and Reconfiguration Analysis of the Trunk Mechanism for a Reconfigurable Wheeled Mobile Platform
Authors: Teng Xinyu , Li Yezhuo , Liu Yang , Yao Yan-an 
Published: February 2024

Abstract
This paper proposes a reconfigurable wheeled mobile platform (RWMP) consisting of two two-wheeled mobile robots and a reconfigurable trunk. The reconfigurable trunk is a 6R multi-mode single-loop mechanism (SLM) that is obtained by inserting two revolute (R) joints with intersected axes into a planar rhombus 4R mechanism. The 6R mechanism has reconfigurable characteristics owing to changes in the wrench system. All six motion modes and their constraint equations of the 6R mechanism are obtained by solving the closed-loop equation based on the D-H transformation matrix. The analysis shows that the mechanism has six single-DOF motion modes, including a planar rhombus 4R mode, two overconstrained spatial 6R modes, and three coaxial 1R modes. The motion characteristics of the six motion modes are identified using screw theory. The six transition configurations among different modes are identified by combining the constraint equations of each mode. The locomotion modes of the RWMP are designed based on the reconfiguration analysis of the trunk mechanism. The locomotion effect of the RWMP under two confined spaces is verified by simulation analysis and prototype experiment.
Self-Reported Depression in Collegiate Athletes: The Effect of Privacy on Symptom Disclosure
Authors: Ouellet-Pizer Chloe M. , Harenberg Sebastian , Vosloo Justine , Meyer Barbara B. 
Published: February 2024

Prevalence studies on depressive symptoms in collegiate athletes have yielded varied estimations, which may be due, in part, to survey administration privacy. However, the influence of survey administration privacy (i.e., anonymous and confidential) on depressive symptom disclosure remains unknown in sport. The purposes of the current study, therefore, were twofold: (a) compare depressive symptoms reported under high- and low-privacy conditions and (b) examine factors associated with underreporting (i.e., social desirability). College athletes (N = 123) were randomly assigned to high- and low-privacy conditions. Results indicated no significant difference, F(1, 120) = 0.59, p = .446, between the prevalence of depressive symptoms reported across conditions when controlling for sex, and no significant correlation between depressive symptoms and social desirability (r = −.01, p = .886). Taken together, results indicated that survey administration privacy did not impact depressive symptom disclosure in the current sample.
Newcastle disease virus activates diverse signaling pathways via Src to facilitate virus entry into host macrophages
Authors: Shi Qiankai , Zhao Ran , Chen Linna , Liu Tianyi , Di Tao , Zhang Chunwei , Zhang Zhiying , Wang Fangfang , Han Zongxi , Sun Junfeng , Liu Shengwang 
Published: February 2024

ABSTRACT

As an intrinsic cellular mechanism responsible for the internalization of extracellular ligands and membrane components, caveolae-mediated endocytosis (CavME) is also exploited by certain pathogens for endocytic entry [e.g., Newcastle disease virus (NDV) of paramyxovirus]. However, the molecular mechanisms of NDV-induced CavME remain poorly understood. Herein, we demonstrate that sialic acid-containing gangliosides, rather than glycoproteins, were utilized by NDV as receptors to initiate the endocytic entry of NDV into HD11 cells. The binding of NDV to gangliosides induced the activation of a non-receptor tyrosine kinase, Src, leading to the phosphorylation of caveolin-1 (Cav1) and dynamin-2 (Dyn2), which contributed to the endocytic entry of NDV. Moreover, an inoculation of cells with NDV-induced actin cytoskeletal rearrangement through Src to facilitate NDV entry via endocytosis and direct fusion with the plasma membrane. Subsequently, unique members of the Rho GTPases family, RhoA and Cdc42, were activated by NDV in a Src-dependent manner. Further analyses revealed that RhoA and Cdc42 regulated the activities of specific effectors, cofilin and myosin regulatory light chain 2, responsible for actin cytoskeleton rearrangement, through diverse intracellular signaling cascades. Taken together, our results suggest that an inoculation of NDV-induced Src-mediated cellular activation by binding to ganglioside receptors. This process orchestrated NDV endocytic entry by modulating the activities of caveolae-associated Cav1 and Dyn2, as well as specific Rho GTPases and downstream effectors.

IMPORTANCE
In general, it is known that the paramyxovirus gains access to host cells through direct penetration at the plasma membrane; however, emerging evidence suggests more complex entry mechanisms for paramyxoviruses. The endocytic entry of Newcastle disease virus (NDV), a representative member of the paramyxovirus family, into multiple types of cells has been recently reported. Herein, we demonstrate the binding of NDV to induce ganglioside-activated Src signaling, which is responsible for the endocytic entry of NDV through caveolae-mediated endocytosis. This process involved Src-dependent activation of the caveolae-associated Cav1 and Dyn2, as well as specific Rho GTPase and downstream effectors, thereby orchestrating the endocytic entry process of NDV. Our findings uncover a novel molecular mechanism of endocytic entry of NDV into host cells and provide novel insight into paramyxovirus mechanisms of entry.
Toward the novel AI tasks in infection biology
Authors: Yakimovich Artur 
Published: February 2024

ABSTRACT

Machine learning and artificial intelligence (AI) are becoming more common in infection biology laboratories around the world. Yet, as they gain traction in research, novel frontiers arise. Novel artificial intelligence algorithms are capable of addressing advanced tasks like image generation and question answering. However, similar algorithms can prove useful in addressing advanced questions in infection biology like prediction of host-pathogen interactions or inferring virus protein conformations. Addressing such tasks requires large annotated data sets, which are often scarce in biomedical research. In this review, I bring together several successful examples where such tasks were addressed. I underline the importance of formulating novel AI tasks in infection biology accompanied by freely available benchmark data sets to address these tasks. Furthermore, I discuss the current state of the field and potential future trends. I argue that one such trend involves AI tools becoming more versatile.
Tidal dissipation morphodynamic feedback triggers loss of microtidal marshes
Authors: Zapp Samuel M. , Mariotti Giulio 
Published: February 2024

Abstract
Coastal marsh loss is commonly attributed to changes in external forcings, such as an increase in sea-level rise rate or a reduction in sediment supply. Here we show that extensive marsh loss can be caused by internal mechanisms alone, and specifically by autogenic tidal choking. This occurs when the marsh fills in, increasing tidal dissipation by bed friction and eventually decreasing the tidal range in its landward section. The reduced tidal range decreases sediment import on the marsh platform and increases ponding, both of which lead to interior marsh loss even with modest sea-level rise rates. This process is predicted to occur in dissipative microtidal marshes, which are experiencing some of the fastest rates of marsh loss worldwide. Considering this mechanism is essential to understanding the relationship between marsh loss, sea-level rise, and sediment supply and to eventually predicting future marsh evolution.
Porcine in vitro digestion and solubilization of non-starch polysaccharides in corn and wheat supplemented with xylanase and feruloyl esterase
Authors: Njeru Harriet K. , Bach Knudsen Knud E. , Woyengo Tofuko A. 
Published: February 2024

A study evaluated porcine in vitro digestion and non-starch polysaccharide (NSP) solubilization of corn and wheat without or with xylanase alone or xylanase plus feruloyl esterase (FE). The enzymes supplied 4000 U of xylanase and 35 U of FE per kilogram of sample. Samples were digested with pepsin at a pH of 2.0 and then with pancreatin at a pH of 6.8. The digested samples were filtered to obtain unhydrolyzed residue, which was then washed using alcohol and acetone. The cereal grains (CGs) before in vitro digestion and the washed unhydrolyzed residues were analyzed for dry matter and NSP. Xylanase improved ( P < 0.05) in vitro digestibility of dry matter (IVDDM) for wheat (85% vs. 89%), but not for corn (78% vs. 79%). Addition of FE to the xylanase-supplemented CGs increased ( P < 0.05) IVDDM for both CGs, and reduced ( P < 0.05) the arabinoxylans in the unhydrolyzed residue for corn, but not for wheat. In conclusion, xylanase product used in the current study is more effective in improving the digestibility of nutrients in wheat than in corn. The FE product used in the current study can improve the efficacy of xylanase in improving the nutrient digestibility of wheat and corn.