bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nursing students satisfaction and self-confidence with standardized patient palliative care simulation focusing on difficult conversations
Authors: Gillan Pauline Catherine , Johnston Sandra 
Published: January 2024

AbstractObjectivesLack of experience communicating with patients and families at the end of life are key concerns for nursing students. Palliative care simulation using standardized patients (SPs) focusing on difficult conversations may lead to increased self-confidence in providing palliative and end-of-life care in clinical practice. There is currently a paucity of research on SP palliative care simulations in undergraduate nursing education. The objective of this research was to assess 3rd year undergraduate nursing students’ levels of satisfaction and self-confidence with palliative and end-of-life care simulations focusing on difficult conversations, as measured by the Student Satisfaction and Self-Confidence in Learning Scale (SSSCLS) and the Simulation Design Scale (SDS).MethodsA descriptive post-intervention study. Four palliative care simulation days, consisting of 2 clinical scenarios, were conducted over 4 weeks. The first simulation was an outpatient palliative care clinic scenario, and the second was an inpatient hospital scenario. Nursing students enrolled in a 3rd year nursing palliative care elective (n = 51) at an Australian university were invited to participate. Students who attended simulation days were eligible to participate (n = 31). Immediately post-simulation, students were invited to complete the SSSCLS and the SDS. Fifty-seven surveys were completed (simulation 1, n = 28; simulation 2, n = 29). The data were analyzed using descriptive statistics.ResultsResults showed that students had high levels of self-confidence in developing palliative care and communication skills after both simulation experiences and high levels of satisfaction with the SP simulations.Significance of resultsThe lack of published literature on palliative care and end-of-life SP simulation highlights the need to collect further evidence to support this as an innovative approach to teaching palliative care. SP palliative care simulation focusing on difficult conversations assists in developing students’ communication skills and improves satisfaction and self-confidence with palliative and end-of-life care.
A New Protocol to Study Mind-Wandering for Air Traffic	Controllers
Authors: Servais Anaïs , Riedinger Florine , Granger Géraud , Imbert Jean-Paul , Barbeau Emmanuel J. , Hurter Christophe 
Published: January 2024

Abstract: Although mind-wandering decreases processing of external information, it remains understudied in air traffic controllers (ATCOs), who must constantly attend to external information. Using autobiographical memory retrieval, we propose an innovative protocol to mimic the moment when ATCOs start mind-wandering. A total of 16 participants performed an ATCO simulation task while we triggered attentional switches toward their inner world using memory probes and recorded eye movements with eye-tracking glasses and a camcorder. We successfully induced one to five spontaneous memories for half of the participants and showed the impact on response times ( Mdn = +0.74 s) to clear flight level. Preliminary results suggest that autobiographical memory could be used to identify a temporal ocular marker – particularly gaze aversion, also positively related to self-reported mind-wandering – to detect the attentional switch.
Evaluation of the Exospheric Temperature Modeling From Different Empirical Orthogonal Functions
Authors: Yang Xu , Weng Libin , Lei Jiuhou , Zhu Xiaoqian , Ruan Haibing , Ren Dexin , Li Zhongli , Li Ruoxi , Chen Liangjie 
Published: January 2024

AbstractIn this paper, we constructed the Exospheric Temperature Models (ETM) on the basis of CHAMP and GRACE data using different empirical orthogonal functions (EOFs). The EOFs of the exospheric temperature can be derived either from satellite data directly or from the outputs of the Thermosphere Ionosphere Electrodynamics General Circulation Model (TIEGCM) and MSIS models by applying the Principal Component Analysis method. Then, the thermospheric mass densities calculated from ETM are used to compare with the observed data in order to evaluate the performance of different ETM models. It was found that all these three models can provide good specification of thermospheric density including day‐night, seasonal, and latitudinal variations. However, the ETM based on CHAMP and GRACE data gives a better performance in modeling the Equatorial Thermospheric Anomaly and the Midnight Density Maximum features than the MSIS‐ETM and TIEGCM‐ETM. Specifically, independent SWARM‐C data comparison showed that the Relative Deviations and corresponding Root‐Mean‐Square‐Errors of our Texo models are less than 8.9% and 22.8%, much better than the MSIS‐00 model.
Hyporheic Flows in Stratified Sediments: Implications on Residence Time Distributions
Authors: Marzadri Alessandra , Ciriello Valentina , de Barros Felipe P. J. 
Published: January 2024

AbstractThe fate of nutrients and contaminants in fluvial ecosystems is strongly affected by the mixing dynamics between surface water and groundwater within the hyporheic zone, depending on the combination of the sediment's hydraulic heterogeneity and dune morphology. This study examines the effects of hydraulic conductivity stratification on steady‐state, two‐dimensional, hyporheic flows and solute residence time distribution. First, we derive an integral transform‐based semi‐analytical solution for the flow field, capable of accounting for the effects of any functional shape of the vertically varying hydraulic conductivity. The solution considers the uneven distribution of pressure at the water‐sediment interface (i.e., the pumping process) dictated by the presence of dune morphology. We then simulate solute transport using particle tracking. Our modeling framework is validated against numerical and tracer data from flume experiments and used to explore the implication of hydraulic conductivity stratification on the statistics and pdf of the residence time. Finally, reduced‐order models are used to enlighten the dependence of key residence time statistics on the parameters characterizing the hydraulic conductivity stratification.
Modulation of learning safety signals by acute stress: paraventricular thalamus and prefrontal inhibition
Authors: Wang Zongliang , Wang Zeyi , Zhou Qiang 
Published: January 2024

AbstractDistinguishing between cues predicting safety and danger is crucial for survival. Impaired learning of safety cues is a central characteristic of anxiety-related disorders. Despite recent advances in dissecting the neural circuitry underlying the formation and extinction of conditioned fear, the neuronal basis mediating safety learning remains elusive. Here, we showed that safety learning reduces the responses of paraventricular thalamus (PVT) neurons to safety cues, while activation of these neurons controls both the formation and expression of safety memory. Additionally, the PVT preferentially activates prefrontal cortex somatostatin interneurons (SOM-INs), which subsequently inhibit parvalbumin interneurons (PV-INs) to modulate safety memory. Importantly, we demonstrate that acute stress impairs the expression of safety learning, and this impairment can be mitigated when the PVT is inhibited, indicating PVT mediates the stress effect. Altogether, our findings provide insights into the mechanism by which acute stress modulates safety learning.
MUC1-C is a target of salinomycin in inducing ferroptosis of cancer stem cells
Authors: Daimon Tatsuaki , Bhattacharya Atrayee , Wang Keyi , Haratake Naoki , Nakashoji Ayako , Ozawa Hiroki , Morimoto Yoshihiro , Yamashita Nami , Kosaka Takeo , Oya Mototsugu , Kufe Donald W. 
Published: January 2024

AbstractThe oncogenic MUC1-C transmembrane protein is a critical effector of the cancer stem cell (CSC) state. Addiction to MUC1-C for self-renewal in the progression of human cancers has emphasized the need for development of anti-MUC1-C agents. However, there are presently no approved small molecules for targeting MUC1-C-dependent CSCs. In screening for small molecules, we identified salinomycin (SAL), an inducer of ferroptosis, as a potent inhibitor of MUC1-C signaling. We demonstrate that SAL suppresses MUC1-C expression by disrupting a NF-κB/MUC1-C auto-inductive circuit that is necessary for ferroptosis resistance. Our results show that SAL-induced MUC1-C suppression downregulates a MUC1-C→MYC pathway that activates genes encoding (i) glutathione-disulfide reductase (GSR), and (ii) the LDL receptor related protein 8 (LRP8), which inhibit ferroptosis by generating GSH and regulating selenium levels, respectively. GSR and LRP8 contribute to the function of glutathione peroxidase 4 (GPX4), an essential negative regulator of ferroptotic cell death. We demonstrate that targeting MUC1-C genetically or with the GO-203 peptide inhibitor suppresses GPX4 expression and GPX activity in association with the induction of ferroptosis. Studies of CSCs enriched by serial passage as tumorspheres further demonstrate that the effects of SAL are mediated by downregulation of MUC1-C and thereby overcoming resistance to ferroptosis. As confirmation of these results, rescue of MUC1-C downregulation with the MUC1-C cytoplasmic domain (i) reversed the suppression of GSR, LRP8 and GPX4 expression, and (ii) attenuated the induction of ferroptosis. These findings identify SAL as a unique small molecule inhibitor of MUC1-C signaling and demonstrate that MUC1-C is an important effector of resistance to ferroptosis.
Mammalian target of rapamycin inhibition protects glioma cells from temozolomide-induced cell death
Authors: Sauer Benedikt , Lorenz Nadja I. , Divé Iris , Klann Kevin , Luger Anna-Luisa , Urban Hans , Schröder Jan-Hendrik , Steinbach Joachim P. , Münch Christian , Ronellenfitsch Michael W. 
Published: January 2024

AbstractGlioblastoma is an incurable brain tumor with a median survival below two years. Trials investigating targeted therapy with inhibitors of the kinase mTOR have produced ambiguous results. Especially combination of mTOR inhibition with standard temozolomide radiochemotherapy has resulted in reduced survival in a phase II clinical trial. To date, this phenomenon is only poorly understood. To recreate the therapeutic setting in vitro, we exposed glioblastoma cell lines to co-treatment with rapamycin and temozolomide and assessed cell viability, DNA damage and reactive oxygen species. Additionally, we employed a novel translatomic based mass spectrometry approach (“mePROD”) to analyze acute changes in translated proteins. mTOR inhibition with rapamycin protected glioblastoma cells from temozolomide toxicity. Following co-treatment of temozolomide with rapamycin, an increased translation of reactive oxygen species (ROS)-detoxifying proteins was detected by mass spectrometry. This was accompanied by improved ROS-homeostasis and reduced DNA damage. Additionally, rapamycin induced the expression of the DNA repair enzyme O-6-methylguanine-DNA methyltransferase (MGMT) in glioblastoma cells with an unmethylated MGMT gene promotor. Inhibition of mTOR antagonized the cytotoxic effects of temozolomide in vitro. The induction of antioxidant defences and MGMT are two underlying candidate mechanisms. Further functional experiments in vitro and in vivo are warranted to characterize this effect that appears relevant for combinatorial therapeutic strategies.
Mechanisms and effects of NLRP3 in digestive cancers
Authors: Si Yuxin , Liu Lei , Fan Zhe 
Published: January 2024

AbstractInflammasomes are thought to be important mediators of host defense against microbial pathogens and maintenance of gastrointestinal tract homeostasis. They can modulate caspase-1 to promote IL-18 and IL-1β secretion and promote phagocytosis induced by bacterial pathogens. NLRP3 is an inflammasome comprising a multiprotein complex assembled by pattern recognition receptors in the cell cytoplasm. It is a crucial component of the innate immune system. Dysregulation of NLRP3 may contribute to inflammatory diseases and intestinal cancers. Recent research suggests that NLRP3 plays an essential role in tumor development; therefore, intensive study of its mechanism is warranted as it could play a key role in the treatment of digestive system tumors. In this review, we discuss the mechanism and role of NLRP3 in tumors of the digestive system and response strategies to modulate NLRP3 for potential use in tumor treatment.
Chromosome-level assembly of Triplophysa yarkandensis genome based on the single molecule real-time sequencing
Authors: She Jiacheng , Chen Shengao , Liu Xuan , Huo Bin 
Published: January 2024

AbstractTriplophysa yarkandensis, a species of freshwater fish endemic to Xinjiang, China, is currently classified as endangered. The objective of this study was to obtain the chromosome-level genome of T. yarkandensis using PacBio and Hi-C techniques. The PacBio sequencing technology resulted in an assembly of 520.64 Mb, with a contig N50 size of 1.30 Mb. Hi-C data was utilized for chromosome mapping, ultimately yielding 25 chromosome sequences. The success rate of chromosome mapping was 93%, with a scaffold N50 of 19.14 Mb, and a BUSCO evaluation integrity of 94.1%. The genome of T. yarkandensis encompasses 25,505 predicted protein-coding genes, with a total of 30,673 proteins predicted. The BUSCO evaluation integrity for predicted protein-coding genes was found to be 91.5%. Additionally, the genome contained a genomic repeat sequence accounting for 27.29% of its total length. Future research employing comparative genomics holds considerable importance in elucidating the molecular mechanisms behind saline-alkali adaptation and ensuring the conservation of biological resources.
Environmental and geographical factors influence the occurrence and abundance of the southern house mosquito, Culex quinquefasciatus, in Hawai‘i
Authors: Villena Oswaldo C. , McClure Katherine M. , Camp Richard J. , LaPointe Dennis A. , Atkinson Carter T. , Sofaer Helen R. , Berio Fortini Lucas 
Published: January 2024

AbstractHawaiian honeycreepers, a group of endemic Hawaiian forest birds, are being threatened by avian malaria, a non-native disease that is driving honeycreepers populations to extinction. Avian malaria is caused by the parasitePlasmodium relictum, which is transmitted by the invasive mosquitoCulex quinquefasciatus. Environmental and geographical factors play an important role in shaping mosquito-borne disease transmission dynamics through their influence on the distribution and abundance of mosquitoes. We assessed the effects of environmental (temperature, precipitation), geographic (site, elevation, distance to anthropogenic features), and trap type (CDC light trap, CDC gravid trap) factors on mosquito occurrence and abundance. Occurrence was analyzed using classification and regression tree models (CART) and generalized linear models (GLM); abundance (count data) was analyzed using generalized linear mixed models (GLMMs). Models predicted highest mosquito occurrence at mid-elevation sites and between July and November. Occurrence increased with temperature and precipitation up to 580 mm. For abundance, the best model was a zero-inflated negative-binomial model that indicated higher abundance of mosquitoes at mid-elevation sites and peak abundance between August and October. Estimation of occurrence and abundance as well as understanding the factors that influence them are key for mosquito control, which may reduce the risk of forest bird extinction.
Non-linear QED approach for betatron radiation in a laser wakefield accelerator
Authors: Ong J. F. , Berceanu A. C. , Grigoriadis A. , Andrianaki G. , Dimitriou V. , Tatarakis M. , Papadogiannis N. A. , Benis E. P. 
Published: January 2024

AbstractLaser plasma-based accelerators provide an excellent source of collimated, bright, and adequately coherent betatron-type x-ray pulses with potential applications in science and industry. So far the laser plasma-based betatron radiation has been described within the concept of classical Liénard–Wiechert potentials incorporated in particle-in-cell simulations, a computing power-demanding approach, especially for the case of multi-petawatt lasers. In this work, we describe the laser plasma-based generation of betatron radiation at the most fundamental level of quantum mechanics. In our approach, photon emission from the relativistic electrons in the plasma bubble is described within a nonlinear quantum electrodynamics (QED) framework. The reported QED-based betatron radiation results are in excellent agreement with similar results using Liénard–Wiechert potentials, as well as in very good agreement with betatron radiation measurements, obtained with multi-10-TW lasers interacting with He and multielectron N$$_2$$

2

gas targets. Furthermore, our QED approach results in a dramatic reduction of the computational runtime demands, making it a favorable tool for designing betatron radiation experiments, especially in multi-petawatt laser facilities.
Synthesis and characterization of a new copper-based polyoxomolybdate and its catalytic activity for azide-alkyne cycloaddition reaction under UV light irradiation
Authors: Amini Mojtaba , Yousofvand Asmaa , Hosseinifard Mojtaba , Bayrami Arshad , Janczak Jan 
Published: January 2024

AbstractA new organic-functionalized Cu-based Anderson-type polyoxomolybdate, namely (C7H15N4)2[Na(H2O)4]2[C6H12CuMo6N2O24]·2(H2O) (CuII-POM), was synthesized via a simple one-pot reaction and subsequently characterized using a range of analytical and spectral techniques. Structural investigation by single crystal X-ray diffraction analysis revealed that the polyanion component of the synthesized compound (i.e. [C6H12CuMo6N2O24]4−) possesses a δ-isomer Anderson-type structure, which is surrounded by four lattice water molecules and four [C7H15N4–NaH15(H2O)8]4+ cations in the crystal packing arrangement. The resulting double-sided tris-functionalized Anderson-type compound can function as highly effective heterogeneous photocatalysts for the copper(I)-catalyzed Huisgen azide-alkyne cycloaddition (Cu-AAC) reaction of terminal alkyne, benzyl halides, and sodium azide (acts as the azidonation and reducing agent) in aqueous media. Ultraviolet light irradiation enhances the catalytic activity of CuII-POM ~ 4.4 times of the “off” situation under reaction conditions of 0.00239 mmol cat., 80 °C, 8 h, 2 mL H2O, So that the isolated yields for the AAC reaction involving a variety of terminal alkynes and benzyl halides using the CuII-POM catalyst ranged between 19–97%. The current study is the first report about using an efficient and economical Cu(II)-POM/UV/NaN3 catalytic system in the Cu-AAC reaction and reveals its significant potential for applying to other Cu(I)-catalyzed reactions.
Trends estimation of obesity prevalence among South Asian young population: a systematic review and meta-analysis
Authors: Bansal Dipika , V. S. Mohammed Safeer , Devi Nagita , Boya Chandrasekhar , Dhora Babu Karamsetty , Dutta Pinaki 
Published: January 2024

AbstractThe premise for effective prevention and treatment of obesity is the availability of accurate prevalence figures. However, the prevalence of pediatric obesity and overweight in South Asian countries has seldom been analyzed. This article provides a comprehensive review and meta-analysis of studies on overweight and obesity to provide a more precise prevalence estimate. The study protocol was registered on PROSPERO (CRD42022320625). PubMed and Embase databases were comprehensively searched from inception till September 2023. The random-effects model was utilized to derive the pooled prevalence of obesity and overweight. Subgroup meta-analysis was used to assess variations in prevalence estimates across subgroups. A meta-regression analysis was also performed to assess the trend of overweight and obesity over the years. 152 studies were included with 489,525 participants. The pooled prevalence was 12.4 (95% CI 11.1–13.6) for overweight, 6.6% (95% CI 5.6–7.8) for obesity, and 19.3% (95% CI 17.1–21.7) for obesity and overweight. In subgroup analysis, Bangladesh reported a higher prevalence for both obesity (8.9%; 95% CI 4.9–13.9) and overweight (13.6%; 95% CI 9.2–18.8). Meta-regression analysis found a significant association between obesity prevalence and the publication year (β = 0.004; p = 0.03; R2 = 2.74%). The results of this study indicate a relatively higher prevalence of childhood obesity in South Asia, emphasizing the necessity for large-scale awareness efforts and context-specific preventative methods.
Visuo-motor lateralization in Apis mellifera: flight speed differences in foraging choices
Authors: Liga Davide , Stancher Gionata , Frasnelli Elisa 
Published: January 2024

AbstractEvidence of lateralization has been provided in Apis mellifera in olfactory learning and social interactions, but not much is known about how it influences visuo-motor tasks. This study investigates visuo-motor biases in free-flying honeybees by analysing left/right choices related to foraging in a Y-maze. Individual bees were trained to associate a visual stimulus (a blue or yellow target) with a reward/punishment: the Blue + group was reinforced for the blue and punished for the yellow, and vice versa for the Yellow + group. In unrewarded tests, we assessed for each bee the directional choice for one of the two identical targets (12 trials with blue targets and 12 with yellow targets) placed in the left and right arms of the maze as well as the flight times to reach the target chosen. The results did not reveal a significant directional preference at the population level, but only at the individual level, with some individuals presenting a strong bias for choosing the right or left stimulus. However, the data revealed an interesting new factor: the influence of both direction and colour on flight times. Overall, bees took less time to choose the stimulus in the left arm. Furthermore, the yellow target, when previously associated with a punishment, was reached on average faster than the punished blue target, with a higher number of no-choices for punished blue targets than for punished yellow targets. This opens new perspectives not only on the study of lateralization in Apis mellifera, but also on the bees’ chromatic preferences.
Detection of caries lesions using a water-sensitive STIR sequence in dental MRI
Authors: Burian Egon , Lenhart Nicolas , Greve Tobias , Bodden Jannis , Burian Gintare , Palla Benjamin , Probst Florian , Probst Monika , Beer Meinrad , Folwaczny Matthias , Schwarting Julian 
Published: January 2024

AbstractIn clinical practice, diagnosis of suspected carious lesions is verified by using conventional dental radiography (DR), including panoramic radiography (OPT), bitewing imaging, and dental X-ray. The aim of this study was to evaluate the use of magnetic resonance imaging (MRI) for caries visualization. Fourteen patients with clinically suspected carious lesions, verified by standardized dental examination including DR and OPT, were imaged with 3D isotropic T2-weighted STIR (short tau inversion recovery) and T1 FFE Black bone sequences. Intensities of dental caries, hard tissue and pulp were measured and calculated as aSNR (apparent signal to noise ratio) and aHTMCNR (apparent hard tissue to muscle contrast to noise ratio) in both sequences. Imaging findings were then correlated to clinical examination results. In STIR as well as in T1 FFE black bone images, aSNR and aHTMCNR was significantly higher in carious lesions than in healthy hard tissue (p < 0.001). Using water-sensitive STIR sequence allowed for detecting significantly lower aSNR and aHTMCNR in carious teeth compared to healthy teeth (p = 0.01). The use of MRI for the detection of caries is a promising imaging technique that may complement clinical exams and traditional imaging.