bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitric oxide as integral element in priming- induced tolerance and plant stress memory
Authors: Fejes Gábor , Bodor Tamás , Szőllősi Réka , Kondak Selahattin , Kutasi Kinga , Fotopoulos Vasileios , Kolbert Zsuzsanna 
Published: January 2025

Abstract
The beneficial effects of priming technology are aimed at the promotion of growth and development and stress tolerance in plants. Different seed pre-treatment and vegetative priming approaches (osmo-, chemical, physical, hormonal, redox treatments) increase the level of nitric oxide (NO) being an active contributor to growth regulation and defence responses. On the other hand, seed pre-treatment or vegetative priming mainly with the NO donor, sodium nitroprusside (SNP) helps to mitigate different abiotic stresses like salinity, cold, drought, excess metals. The effect of SNP/NO covers the alleviation of stress-specific effects (e.g. reduction of cadmium uptake in case of cadmium loading, improvement of water balance in case of drought), as well as general effects, such as alleviating oxidative stress. Seed pre-treatment or vegetative priming with SNP/NO up-regulates antioxidant enzymes at the activity level and increases the amount of a wide range of non-enzymatic antioxidants. However, due to adverse effects of SNP we urge the testing of other conventional NO donors (e.g. S-nitrosoglutathione) as well as new substances with more favourable properties (e.g. NO-releasing nanomaterials, plasmas, plasma treated liquids, combined donors) as seed pre-treating and vegetative priming materials. This review gives further suggestions on the methodology and future directions of fundamental research in relation to NO-associated pre-treatment and priming.
Intestinal Bacteroides drives glioma progression by regulating CD8+ T cell tumor infiltration
Authors: Chatterjee Jit , Qi Xuanhe , Mu Rui , Li Xuanwei , Eligator Talia , Ouyang Megan , Bozeman Stephanie L , Rodgers Rachel , Aggarwal Somya , Campbell Danielle E , Schriefer Lawrence A , Baldridge Megan T , Gutmann David H 
Published: January 2025

Abstract

Background
The intestinal microbiota regulates normal brain physiology and the pathogenesis of several neurological disorders. While prior studies suggested that this regulation operates through immune cells, the underlying mechanisms remain unclear. Leveraging two well characterized murine models of low-grade glioma (LGG) occurring in the setting of the neurofibromatosis type 1 (NF1) cancer predisposition syndrome, we sought to determine the impact of the gut microbiome on optic glioma progression.

Methods
Nf1-mutant mice genetically engineered to develop optic pathway gliomas (Nf1OPG mice) by 3 months of age were reared under germ-free (GF) conditions, treated with specific cocktails of antibiotics, or given fecal matter transplants (FMTs). Intestinal microbial species were identified by 16S genotyping. Neutralizing TGFβ antibodies were delivered systemically, while in vitro experiments used isolated murine microglia and T cells. Single cell RNA sequencing analysis was performed using established methods.

Results
Nf1 OPG mice raised in a GF environment or postnatally treated with vancomycin did not harbor optic gliomas or exhibit OPG-induced retinal nerve fiber layer thinning, which was reversed following conventionally raised mouse FMT or colonization with Bacteroides species. Moreover, this intestinal microbiota-regulated gliomagenesis was mediated by circulating TGFβ, such that systemic TGFβ neutralization reduced Nf1-OPG growth. TGFβ was shown to act on tumor-associated monocytes to induce Ccl3 expression and recruit CD8+ T cells necessary for glioma growth.

Conclusions
Taken together, these findings establish, for the first time, a mechanistic relationship between Bacteroides in the intestinal microbiome and NF1-LGG pathobiology, suggesting both future predictive risk assessment strategies and therapeutic opportunities.
Isopropylmalate synthase regulatory domain removal abolishes feedback regulation at the expense of leucine homeostasis in plants
Authors: Varghese Mohan , Kumar Roshan , Sharma Aprajita , Lone Asif , Gershenzon Jonathan , Bisht Naveen C 
Published: January 2025

Abstract
In the leucine (Leu) biosynthesis pathway, homeostasis is achieved through a feedback regulatory mechanism facilitated by the binding of the end product Leu at the C-terminal regulatory domain of the first committed enzyme, isopropylmalate synthase (IPMS). In vitro studies have shown that removing the regulatory domain abolishes the feedback regulation on plant IPMS while retaining its catalytic activity. However, the physiological consequences and underlying molecular regulation of Leu flux upon removing the IPMS regulatory domain remain to be explored in plants. Here, we removed the IPMS C-terminal regulatory domain using a CRISPR/Cas9-based gene editing system and studied the resulting impact on the Leu biosynthesis pathway under in planta conditions. Absence of the IPMS regulatory domain unexpectedly reduced the formation of the end product Leu but increased the levels of Leu pathway intermediates in mustard (Brassica juncea). Additionally, delayed growth was observed when IPMS devoid of the regulatory domain was introduced into IPMS-null mutants of Escherichia coli and Arabidopsis thaliana. Further, a detailed biochemical analysis showed that in the absence of the C-terminal regulatory domain, a Leu pathway intermediate (α-ketoisocaproate) could compete with the native IPMS substrate (2-oxoisovalerate) for the active site. Combining these metabolomic, biochemical, and in planta analyses, we demonstrate that the C-terminal regulatory domain of IPMS is critical for maintaining Leu–Val homeostasis in plants.
The Financial Consequences of Pretrial Detention
Authors: Slutzky Pablo , Xu Sheng-Jun 
Published: January 2025

Abstract
In the United States, a significant number of criminal defendants are held in pretrial detention and face substantial financial burdens. Matching individual-level criminal case records to household-level financial data, we exploit the quasi-random assignment of court commissioners to study how pretrial detention affects household solvency. We find that pretrial detention results in higher rates of household insolvency, driven by higher rates of chapter 7 bankruptcies and judgment liens, and higher foreclosure rates during periods of decreasing house prices. We document that the effects spill over to family members and show that home equity can cushion households from insolvency. (JEL D14, G51, K41, K42)
Cognitive Impairment in Psychotic Disorders Is Associated with Brain Reductive Stress and Impaired Energy Metabolism as Measured by 31P Magnetic Resonance Spectroscopy
Authors: Chouinard Virginie-Anne , Du Fei , Chen Xi , Tusuzian Emma , Ren Boyu , Anderson Jacey , Cuklanz Kyle , Feizi Wirya , Zhou Shuqin , Weerasekera Akila , Cohen Bruce M , Öngür Dost , Lewandowski Kathryn E 
Published: January 2025

Abstract

Background and Hypothesis
Convergent evidence shows the presence of brain metabolic abnormalities in psychotic disorders. This study examined brain reductive stress and energy metabolism in people with psychotic disorders with impaired or average range cognition. We hypothesized that global cognitive impairment would be associated with greater brain metabolic dysregulation.

Study Design
Participants with affective and non-affective psychosis (n = 62) were administered the MATRICS Consensus Cognitive Battery (MCCB) and underwent a 31P-magnetic resonance spectroscopy scan at 4T. We used a cluster-analysis approach to identify 2 clusters of participants with and without cognitive dysfunction. We compared clusters on brain redox balance or reductive stress, measured by the ratio of nicotinamide adenine dinucleotide (NAD+) and its reduced form NADH, in addition to creatine kinase (CK) enzymatic activity and pH.

Study Results
The mean (SD) age of participants was 25.1 (6.3) years. The mean NAD+/NADH ratio differed between groups, with lower NAD+/NADH ratio, suggesting more reductive stress, in the impaired cognitive cluster (t = −2.60, P = .01). There was also a significant reduction in CK activity in the impaired cognitive cluster (t = −2.19, P = .03). Intracellular pH did not differ between the 2 cluster groups (t = 1.31, P = .19). The clusters did not significantly differ on severity of mood and psychotic symptomatology or other measures of illness severity.

Conclusions
Our results demonstrate that psychotic disorders with greater cognitive impairment have greater brain metabolic dysregulation, with more reductive stress and decrease in energy metabolic rate markers. This provides new evidence for the potential of emerging metabolic therapies to treat cognitive deficits in psychotic disorders.
Reverse-flow Flaps of the Medial Plantar Region: Review of the Literature With Case Report
Authors: Yuen James C. , Shahare Humam M. , Hicks Evan D. 
Published: January 2025

Background:
Many variations of the reverse flap design elevated on the medial plantar region have emerged since its introduction. Our aim was to review the literature to provide a broader understanding of the various iterations of the reversed blood-flow flap raised on the medial plantar region. Second, we wished to appraise the reverse medialis pedis flap by review of the literature and presentation of a case report.

Methods:
A review of the literature was conducted regarding the reversed blood-flow flap elevated in the medial plantar region. The diverse types of vascular axis and retrograde blood-flow origin of the reverse flap in the medial plantar region were categorized. A clinical case of the reverse medialis pedis flap with step-by-step flap elevation and description of key landmarks are presented.

Results:
Our literature review identified 38 publications pertaining to retrograde flow flaps in the medial plantar region. The reverse-flow anatomy derived from these publications was categorized based on vascular axis (pedicle) and retrograde blood-flow origins; Only 5 publications included the reverse medialis pedis flap in their article while 3 papers focused on detailed anatomic study and case series on this flap.

Conclusions:
The reverse medial plantar artery flaps come with multiple flap designs depending on which antegrade flow artery is ligated and what anastomotic vascular origin is supplying the reversed blood flow. The reverse medialis pedis flap based on the superficial medial plantar artery with anastomosis to the first plantar metatarsal artery deserves consideration as an option for small defects of the great toe metatarsophalangeal joint.
Use of Magnetic Resonance Neurography for Sensory Nerve Injuries of the Head and Neck
Authors: Hazewinkel Merel H.J. , Lin Yenpo , Li Tim Y. , Cisse Babacar , Tan Ek T. , Sneag Darryl B. , Gfrerer Lisa 
Published: January 2025

Background:
Identification of peripheral nerve injuries of the head and neck can be challenging due to a broad spectrum of symptoms from neuropathic pain to headaches and migraine. This article aimed to present the clinical features and diagnostic workup of patients with acute and chronic peripheral nerve injuries of the head and neck using magnetic resonance neurography (MRN), to demonstrate potential advantages compared with conventional magnetic resonance imaging (MRI).

Methods:
Patients who presented with suspected peripheral nerve injury were either referred for a conventional MRI or MRN. Patients who underwent nerve exploration for suspected nerve transection and/or neuroma formation on imaging were included in this study. Imaging findings were correlated with intraoperative observations.

Results:
Four patients (3 women, 1 man, age range: 34–70 years) were included. Three subjects had a history of head and neck surgery and 1 experienced direct trauma to the medial eyebrow. Clinical symptoms included numbness, allodynia, positive Tinel sign, and pain relief following nerve blocks. Two patients underwent conventional MRI and 2 underwent MRN. MRI provided a vague indication of potential neuromas and failed to accurately depict their locations. MRN offered a comprehensive visualization of the entire nerve path, identifying nerve transection and neuromas, as well as precise location, dimensions, and relation to adjacent bones and muscles.

Conclusions:
High-resolution 3-dimensional MRN provides clear visualization of acute and chronic peripheral nerve injuries of the head and neck region, facilitating early diagnosis of nerve injuries in this region and improving diagnostic accuracy, as well as surgical planning and execution.
A New Anatomical Approach to Male Pectoral Volumization
Authors: Flores González Emmanuel Armando , Babaitis Ricardo , Manzaneda Cipriani Raul Martin , Schneider Viaro Mauricio , Duran Vega Hector Cesar , Lobo Botelho Daniel , Ramírez Guerrero Oliver René , Gracida Mancilla Noe Isaias 
Published: January 2025

Background:
In this article, we discuss a new technique of pectoralis major muscle volumization using ultrasound-guided fat grafting, considering a new surgical criterion that allows for larger volumes.

Methods:
We present a retrospective observational descriptive study, which included men who underwent body contouring surgery during 2022–2023. As part of the study, measurements were taken before, immediately after, and at 3 months after surgery. Data were stored in a database and analyzed using SPSS statistical software, v23.0.

Results:
The average age was 34.8 years [±8.2] and when evaluating the cases based on their body mass index, we found an average body mass index of 28.2 kg/m2 [±1.9]. When evaluating the paired differences between initial depth of pectoralis major–transoperative depth of pectoralis major, transoperative depth of pectoralis major–postoperative depth of pectoralis major at 3 months, and initial depth of pectoralis major–postoperative depth of pectoralis major at 3 months using the Student t statistic for related samples, statistically significant differences were obtained (P = 0.001). This is a preliminary short-term study revealing a new approach to volumizing the pectoralis major muscle and requires long-term follow-up.

Conclusions:
The technique allows for an increase in muscle thickness. Measurements were taken immediately after infiltration and at 3 months, observing the presence of the fat graft and the increase in volume. In the future, it is important to perform long-term follow-up. No adverse events occurred during the study.
Characteristics and Surgical Outcomes of Laser-induced Full-thickness Macular Holes
Authors: Cui Ying , Liu Wu , Wang Yaxing , Shi Xiangyu 
Published: January 2025

Purpose:
To report the characteristics and the surgical outcomes of full-thickness macular holes (MHs) accidentally caused by laser devices and analyze the visual predictors.

Methods:
33 eyes that underwent vitrectomy for laser-induced MHs were included. Full ophthalmic examinations and optical coherence tomography were used for assessment at baseline and at follow-ups. Preoperative and 6-months postoperative features were analyzed respectively as potential predictors of final visual acuity.

Results:
11 eyes were injured by a neodymiumdoped yttrium aluminum garnet (Nd:YAG) laser and 22 by a handheld laser. 5/33 eyes had a focal subfoveal choroidal depression preoperatively. MH closure was achieved in 87.9% eyes. 79.3% eyes had VA improvement of 2 or more lines. Compromised VA improvement was associated with preoperative choroidal depression (P=0.026, odds ratio [OR]=0.068, 95% confidence interval [CI]: 0.006∼0.729). Worse final BCVA was associated with worse preoperative BCVA (P=0.001, regression coefficient [B]=0.497, 95%CI: 0.221∼0.774), preoperative choroidal depression (P=0.014, B=0.353, 95%CI: 0.078∼0.628), Nd:YAG laser injury (P=0.030, B=-0.244, 95%CI: -0.461∼-0.026), larger MH diameter (P=0.023, B=0.031, 95%CI: 0.005∼0.058), failed macular hole closure (P=0.003, B=-0.472, 95%CI: -0.775∼-0.169), larger ellipsoid zone (EZ) defect at 6 months (P=0.002, B=0.053, 95%CI: 0.024∼0.082) and thinner foveal neuroretina thickness at 6 months (P<0.001, B=-0.365, 95%CI: -0.520∼-0.211).

Conclusion:
Vitrectomy is effective in closing laser-induced MHs and improving visual acuity. Preoperative factors including worse preoperative BCVA, choroidal depression, Nd:YAG laser injury and larger MH diameter and postoperative factors including failed MH closure, larger EZ defect and thinner foveal neuroretina at 6 months are predictive factors for worse final VA.
Repurposing Diflunisal as an Anti-Virulence Agent Against Staphylococcus aureus
Authors: Sun Daniel , Haste Nina M. , Sun Josh , Serafim Mateus Sá Magalhães , Salvioni Anna , Olson Joshua , Cole Jason , Okumura Cheryl , Gallo Richard L. , Sakoulas George , O’Donoghue Anthony J. , Hensler Mary E. , Nizet Victor 
Published: January 2025

Abstract
Infections refractory to standard antibiotic therapy are contributing to adverse treatment outcomes in patients suffering from deep-seated bacterial infections caused by increasingly resistant pathogens. Adjunctive strategies targeting bacterial virulence factors have been considered to supplement the host immune response in fighting the infection. Previous studies suggest that the FDA-approved anti-inflammatory drug diflunisal inhibits Staphylococcus aureus (SA) α-toxin expression by its interaction with the response regulator AgrA. We investigated the broader anti-virulence properties of diflunisal against pathogenic strains of SA and established proof-of-concept for its efficacy in blocking SA virulence. Our studies reveal that diflunisal inhibits α-toxin production, sensitizes SA to cationic antibiotics and human antimicrobial peptides, inhibits the production of the golden pigment staphyloxanthin, and reduces biofilm formation. Molecular docking simulations revealed potential interactions between diflunisal and AgrA binding sites. In addition, sequence alignment of the SA AgrA response regulator demonstrated similarities to other response regulators involved in controlling virulence factor expression. Appreciation of the antivirulence properties of diflunisal supports a therapeutic strategy distinct from structurally similar compounds, such as salicylic acid. The repurposing of diflunisal may mitigate disease severity and provide a unique adjunctive tool in the treatment of SA infection.
Early-life adversity as a predictor of fibromyalgia syndrome: the central role of perceived stress over endocrine stress indicators
Authors: Beiner Eva , Hermes Michelle , Reichert Julian , Kleinke Kristian , Vock Stephanie , Löffler Annette , Ader Leonie , Sirazitdinov Andrei , Keil Sebastian , Schmidt Tim , Schick Anita , Löffler Martin , Hopp Michael , Ruckes Christian , Hesser Jürgen , Reininghaus Ulrich , Flor Herta , Eich Wolfgang , Tesarz Jonas 
Published: January 2025

Abstract
This study investigates the associations between early childhood adversities, stress perception, and fibromyalgia syndrome (FMS). Although the interconnection between dysregulated stress systems and FMS is well documented, the interconnection between early adversities and FMS remains less understood. This study explores the relationship of early-life stress and FMS by examining its mediation through perceived stress, and acute and chronic endocrine stress indicators. Stress was assessed using the perceived stress scale, as well as using salivary and hair cortisol as endocrine indicators of acute and chronic stress, respectively. The sample consisted of 99 individuals with FMS and 50 pain-free controls. A structural equation model was used to assess the mediating effects of stress indicators between early adversities and the severity of FMS. Compared with controls, individuals with FMS had notably higher early adversity scores (d = 0.63) and greater occurrence of exposure to adversity (78.8% vs 66%). Structural equation modeling indicated that the influence of early adversities on FMS symptoms is mediated by perceived stress levels, with no direct effect observed. Our findings indicate that early-life adversity is a significant determinant of the development of FMS, with the relationship between these factors mediated by perceived stress rather than by endocrine stress indicators. These results underscore the critical role of stress perception in the development and management of FMS, suggesting that perceived stress may serve as a valuable therapeutic target. Incorporating trauma-informed and stress-targeted care into treatment strategies could significantly improve outcomes for individuals with FMS, emphasizing the importance of addressing psychological factors alongside physical symptoms.
Clinical value of aortic arch morphology in transfemoral TAVR: artificial intelligence evaluation
Authors: Mao Yu , Liu Yang , Zhai Mengen , Jin Ping , Chen Fangyao , Yang Yuhui , Zhu Guangyu , Yang Tingting , Zhang Gejun , Xu Kai , Shang Xiaoke , Zhao Yuan , Ni Buqing , Li Hongxin , Tang Min , Jian Zhao , Yang Yining , Zhang Haibo , Wei Lai , Liu Jian , Noterdaeme Timothée , Lange Ruediger , Guo Yingqiang , Pan Xiangbin , Wu Yongjian , Yang Jian 
Published: January 2025

Background:
The impact of aortic arch (AA) morphology on the management of the procedural details and the clinical outcomes of the transfemoral artery (TF)-transcatheter aortic valve replacement (TAVR) has not been evaluated. The goal of this study was to evaluate the AA morphology of patients who had TF-TAVR using an artificial intelligence algorithm and then to evaluate its predictive value for clinical outcomes.

Materials and methods:
A total of 1480 consecutive patients undergoing TF-TAVR using a new-generation transcatheter heart valve at 12 institutes were included in this retrospective study. The AA measurements were evaluated by deep learning, and then the approach index (IA) was determined. The machine learning algorithm was used to construct the predictive model and was validated externally.

Results:
The area under the curve of the IA model using random forest and logistic regression was 0.675 [95% confidence interval (CI): 0.586–0.764] and 0.757 (95% CI: 0.665–0.849), respectively. The IA model was validated externally, and consistent distinctions were obtained. After we used a generalized propensity score matching method for continuous exposure, the IA was the strongest correlation factor for major procedural events (odds ratio: 3.87; 95% CI: 2.13–7.59, P < 0.001). When leaflet morphology or transcatheter heart valve type was an interactive item with IA, neither of them was statistically significant in terms of clinical outcomes.

Conclusion:
IA may be used to identify the impact of AA morphology on procedural and clinical outcomes in patients having TF-TAVR and to help to predict the procedural complications.