bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Wintertime Arctic Oscillation Signature on Early-Season Indian Ocean Monsoon Intensity
Authors: Buermann Wolfgang , Lintner Benjamin , Bonfils Céline 
Published: August 2005

AbstractThe Indian Ocean monsoon (IOM) exhibits considerable year-to-year variations that have previously been attributed to a number of forcing mechanisms including the El Niño–Southern Oscillation (ENSO) and Eurasian snow cover anomalies. In this study, spatial data of Eurasian spring land surface temperatures are analyzed as well as proxies for soil moisture, summer IOM precipitation, and summer IOM 850-mb zonal winds for the 1979–99 period to isolate correlated modes of variability. The results indicate the existence of a prominent mode that appears to be related to the boreal winter Arctic Oscillation (AO); this mode projects strongly on the June precipitation and 850-mb zonal wind fields in the vicinity of the IOM region. Its projection on spatial fields of temperature and proxies for soil moisture shows springtime surface warming and drying in the region to the north and west of the Indian subcontinent and cooling over the higher Eurasian latitudes during years of anomalously intense June monsoon rainfall. Such surface signatures are consistent with the negative phase winter AO. It is hypothesized that the preconditioning of the spring season surface characteristics may be associated with an AO-induced quasi-stationary tropospheric circulation anomaly: the impact of this anomaly is to displace the mid-Eastern jet poleward during AO-negative phases, resulting in anomalous surface heating and drying that persist into the later spring season and finally affect the rainfall over the IOM region in June.
Seasonal Variability in Clouds and Radiation at the Manus ARM Site
Authors: Mather James H. 
Published: August 2005

Abstract
The Atmospheric Radiation Measurement (ARM) program operates three climate observation stations in the tropical western Pacific region. One of these sites, located on Manus Island in Papua New Guinea, has been operating since 1996. The Manus ARM site includes an extensive array of instruments chosen to observe cloud properties, water vapor and temperature profiles, and the surface radiation budget. This dataset provides an opportunity to examine variability in tropical cloudiness on a wide range of time scales. The focus of this study is on the annual cycle. Analysis of cloud distribution and radiation data from Manus reveals a clear annual cycle in clouds associated with convective activity. The most convectively active period is found to be the Northern Hemisphere summer, while the least active period is the Northern Hemisphere autumn. Outgoing longwave radiation (OLR) data are also examined in order to relate observations at Manus with the surrounding region. Significant differences are found between the annual cycle at Manus and adjacent large islands within the Maritime Continent. Analysis of the combined ARM–OLR data suggests that during the Northern Hemisphere winter, a significant amount of the high clouds observed over Manus are associated with continental convection over the large Maritime Continent islands.
Pilot study of losartan for pulmonary hypertension in chronic obstructive pulmonary disease
Authors: Morrell Nicholas W , Higham Matthew A , Phillips Peter G , Shakur B Haleema , Robinson Paul J , Beddoes Ray J 
Published: August 2005

AbstractBackgroundMorbidity in COPD results from a combination of factors including hypoxia-induced pulmonary hypertension, in part due to pulmonary vascular remodelling. Animal studies suggest a role of angiotensin II and acute studies in man concur. Whether chronic angiotensin-II blockade is beneficial is unknown. We studied the effects of an angiotensin-II antagonist losartan, on haemodynamic variables, exercise capacity and symptoms.MethodsThis was a double-blind, randomized, parallel group, placebo- controlled study of 48 weeks duration. Forty patients with COPD and pulmonary hypertension (Tran tricuspid pressure gradient (TTPG) = 30 mmHg) were randomised to losartan 50 mg or placebo. Changes in TTPG were assessed at 3, 6 and 12 months.ResultsThere was a trend for TTPG to increase in the placebo group (baseline 43.4 versus 48.4 mmHg at endpoint) and stay constant in the losartan group (baseline 42.8 versus 43.6 mmHg). More patients in the losartan group (50%) than in the placebo group (22%) showed a clinically meaningful reduction in TTPG at any timepoint; these effects seemed more marked in patients with higher baseline TTPG. There were no clear improvements in exercise capacity or symptoms.ConclusionIn this 12-month pilot study, losartan 50 mg had no statistically significant beneficial effect on TTPG, exercise capacity or symptoms in pulmonary hypertension secondary to obstructive disease. A sub-group of patients with higher TTPG may benefit.
Assessing, treating and preventing community acquired pneumonia in older adults: findings from a community-wide survey of emergency room and family physicians
Authors: Krueger Paul , Loeb Mark , Kelly Caralyn , Edward H Gayle 
Published: August 2005

Abstract

Background
Respiratory infections, like pneumonia, represent an important threat to the health of older Canadians. Our objective was to determine, at a community level, family and emergency room physicians' knowledge and beliefs about community acquired pneumonia (CAP) in older adults and to describe their self-reported assessment, management and prevention strategies.

Methods
All active ER and family physicians in Brant County received a mailed questionnaire. An advance notification letter and three follow-up mailings were used to maximize physician participation rate. The questionnaire collected information about physicians' assessment, management, and prevention strategies for CAP in older adults (≥60 years of age) plus demographic, training, and practice characteristics. The analysis highlights differences in approaches between office-based and emergency department physicians.

Results
Seventy-seven percent of physicians completed and returned the survey. Although only 16% of physicians were very confident in assessing CAP in older adults, more than half reported CAP to be a very important health concern in their practices. In-service training for family physicians was associated with increased confidence in CAP assessment and more frequent use of diagnostic tests. Family physicians who reported always requesting chest x-rays were also more likely to request pulse oximetry (OR 5.6, 95% CI 1.40 to 22.5) and recommend both follow-up x-rays (OR 5.4, 95% CI 1.7 to 16.6) and pneumococcal vaccination (OR 3.4, 95% CI 1.1 to 10.0).

Conclusion
The findings of this study provide a snapshot of how non-specialists from a non-urban Ontario community assess, manage and prevent CAP in older adults and highlight differences between office-based and emergency department physicians. This information can guide researchers and clinicians in their efforts to improve the management and prevention of CAP in older adults.
Audio computer-assisted self-interviewing (ACASI) may avert socially desirable responses about infant feeding in the context of HIV
Authors: Waruru Anthony K , Nduati Ruth , Tylleskär Thorkild 
Published: August 2005

Abstract
Background
Understanding infant feeding practices in the context of HIV and factors that put mothers at risk of HIV infection is an important step towards prevention of mother to child transmission of HIV (PMTCT). Face-to-face (FTF) interviewing may not be a suitable way of ascertaining this information because respondents may report what is socially desirable. Audio computer-assisted self-interviewing (ACASI) is thought to increase privacy, reporting of sensitive issues and to eliminate socially desirable responses. We compared ACASI with FTF interviewing and explored its feasibility, usability, and acceptability in a PMTCT program in Kenya.

Methods
A graphic user interface (GUI) was developed using Macromedia Authorware® and questions and instructions recorded in local languages Kikuyu and Kiswahili. Eighty mothers enrolled in the PMTCT program were interviewed with each of the interviewing mode (ACASI and FTF) and responses obtained in FTF interviews and ACASI compared using McNemar's χ2 for paired proportions. A paired Student's t-test was used to compare means of age, marital-time and parity when measuring interview mode effect and two-sample Student's t-test to compare means for samples stratified by education level – determined during the exit interview. A Chi-Square (χ2test) was used to compare ability to use ACASI by education level.

Results
Mean ages for intended time for breastfeeding as reported by ACASI were 11 months by ACASI and 19 months by FTF interviewing (p < 0.001). Introduction of complementary foods at ≤3 months was reported more frequently by respondents in ACASI compared to FTF interviews for 7 of 13 complementary food items commonly utilized in the study area (p < 0.05). More respondents reported use of unsuitable utensils for infant feeding in ACASI than in FTF interviewing (p = 0.001). In other sensitive questions, 7% more respondents reported unstable relationships with ACASI than when interviewed FTF (p = 0.039). Regardless of education level, respondents used ACASI similarly and majority (65%) preferred it to FTF interviewing mainly due to enhanced usability and privacy. Most respondents (79%) preferred ACASI to FTF for future interviewing.

Conclusion
ACASI seems to improve quality of information by increasing response to sensitive questions, decreasing socially desirable responses, and by preventing null responses and was suitable for collecting data in a setting where formal education is low.
A comparison of six analytical disease mapping techniques as applied to West Nile Virus in the coterminous United States
Authors: Griffith Daniel A 
Published: August 2005

AbstractWest Nile Virus has quickly become a serious problem in the United States (US). Its extremely rapid diffusion throughout the country argues for a better understanding of its geographic dimensions. Both 2003 and 2004 percentages of deaths by numbers of reported human cases, for the 48 coterminous US states, are analyzed with a range of spatial statistical models, seeking to furnish a fuller appreciation of the variety of models available to researchers interested in analytical disease mapping. Comparative results indicate that no single spatial statistical model specification furnishes a preferred description of these data, although normal approximations appear to furnish some questionable implications. Findings also suggest several possible future research topics.
Role of early experience in ant enslavement: a comparative analysis of a host and a non-host species
Authors: Blatrix Rumsaïs , Sermage Claire 
Published: August 2005

Abstract

Background
Ants use the odour of the colony to discriminate nestmates. In some species, this odour is learned during the first days following emergence, and thus early experience has a strong influence on nestmate discrimination. Slave-making ants are social parasites that capture brood of other ant species to increase the worker force of their colony. After emerging in the slave-maker nest, slave workers work as if they were in their own colony. We tested the hypothesis that early experience allows the deception of commonly enslaved species, while non-host species use a different mechanism, which does not involve learning.

Results
Pupae of a host species, Temnothorax unifasciatus, and a non-host species, T. parvulus, were allowed to emerge in the presence of workers of one of two slave-maker species, Chalepoxenus muellerianus or Myrmoxenus ravouxi. When T. unifasciatus was exposed to slave-makers for 10 days following emergence, they were more aggressive towards their own sisters and groomed the slave-maker more. T. parvulus gave a less clear result: while workers behaved more aggressively towards their sisters when exposed early to C. muellerianus workers, this was not the case when exposed early to M. ravouxi workers. Moreover, T. parvulus workers allogroomed conspecific nestmates less than T. unifasciatus. Allogrooming activity might be very important for the slave-makers because they are tended by their slaves.

Conclusion
Our findings show that early experience influences nestmate discrimination in the ant T. unifasciatus and can account for the successful enslavement of this species. However, the non-host species T. parvulus is less influenced by the early environment. This might help to explain why this species is never used by social parasites.
An evaluation of the metabolic syndrome in a large multi-ethnic study: the Family Blood Pressure Program
Authors: Kraja Aldi T , Rao DC , Weder Alan B , Mosley Thomas H , Turner Stephen T , Hsiung Chao Agnes , Quertermous Thomas , Cooper Richard , Curb J David , Province Michael A 
Published: August 2005

Abstract

Background
The Family Blood Pressure Program is an ongoing, NHLBI-sponsored, multi-center program to study the genetic determinants of high blood pressure. The goal of this particular study was to study patterns of metabolic syndrome (MetS) in four ethnic groups: African Americans, Caucasians, Hispanics, and Asians.

Methods
A major part of participants in three networks GENOA, HyperGEN and SAPPHIRe were recruited mainly through hypertensive probands. MetS was defined as a categorical trait following the National Cholesterol Education Program definition (c-MetS). MetS was also characterized quantitatively through multivariate factor analyses (FA) of 10 risk variables (q-MetS). Logistic regression and frequency tables were used for studying associations among traits.

Results
Using the NCEP definition, the Hispanic sample, which by design was enriched for type 2 diabetes (T2D), had a very high prevalence of MetS (73%). In contrast, its prevalence in Chinese was the lowest (17%). In African Americans and Hispanics, c-MetS was more prevalent in women than in men. Association of c-MetS with type 2 diabetes (T2D) was prominent in the Hispanics and African Americans, less pronounced in the Whites and Japanese, (although still significant), and weakest in the Chinese sample.
Using FA without rotation, we found that the main factor loaded obesity (OBS) and blood pressure (BP) in African Americans; OBS and insulin (INS) in Hispanics, in Japanese, and in Whites; and OBS alone in Chinese. In Hispanics, Whites, and Japanese, BP loaded as a separate factor. Lipids in combination with INS also loaded in a separate factor. Using FA with Varimax rotation, 4 independent factors were identified: "Obesity-INS," "Blood pressure," "Lipids-INS," and "Central obesity." They explained about 60% of the variance present in the original risk variables.

Conclusion
MetS ethnic differences were identified. Ascertaining for hypertension or T2D increased the MetS prevalence in networks compared with the one in the US general population. Obesity was the most prominent risk factor contributing to both c-MetS and q-MetS. INS contributed in two important factors (obesity and lipids). The information imbedded into c-MetS trait /q-MetS factors scores can contribute in future research of the MetS, especially its utilization in the genetic analysis.
Celecoxib analogues disrupt Akt signaling, which is commonly activated in primary breast tumours
Authors: Kucab Jill E , Lee Cathy , Chen Ching-Shih , Zhu Jiuxiang , Gilks C Blake , Cheang Maggie , Huntsman David , Yorida Erika , Emerman Joanne , Pollak Michael , Dunn Sandra E 
Published: August 2005

Abstract
Introduction
Phosphorylated Akt (P-Akt) is an attractive molecular target because it contributes to the development of breast cancer and confers resistance to conventional therapies. Akt also serves as a signalling intermediate for receptors such as human epidermal growth factor receptor (HER)-2, which is overexpressed in 30% of breast cancers; therefore, inhibitors to this pathway are being sought. New celecoxib analogues reportedly inhibit P-Akt in prostate cancer cells. We therefore examined the potential of these compounds in the treatment of breast cancer. The analogues were characterized in MDA-MB-453 cells because they overexpress HER-2 and have very high levels of P-Akt.

Methods
To evaluate the effect of the celecoxib analogues, immunoblotting was used to identify changes in the phosphorylation of Akt and its downstream substrates glycogen synthase kinase (GSK) and 4E binding protein (4EBP-1). In vitro kinase assays were then used to assess the effect of the drugs on Akt activity. Cell death was evaluated by poly(ADP-ribose) polymerase cleavage, nucleosomal fragmentation and MTS assays. Finally, tumour tissue microarrays were screened for P-Akt and HER-2 expression.

Results
OSU-03012 and OSU-O3013 inhibited P-Akt and its downstream signalling through 4EBP-1 and GSK at concentrations well below that of celecoxib. Disruption of P-Akt was followed by induction of apoptosis and more than 90% cell death. We also noted that the cytotoxicity of the celecoxib analogues was not significantly affected by serum. In contrast, the presence of 5% serum protected cells from celecoxib induced death. Thus, the structural modification of the celecoxib analogues increased P-Akt inhibition and enhanced the bioavailability of the drugs in vitro. To assess how many patients may potentially benefit from such drugs we screened tumour tissue microarrays. P-Akt was highly activated in 58% (225/390) of cases, whereas it was only similarly expressed in 35% (9/26) of normal breast tissues. Furthermore, HER-2 positive tumours expressed high levels of P-Akt (P < 0.01), supporting in vitro signal transduction.

Conclusion
We determined that Celecoxib analogues are potent inhibitors of P-Akt signalling and kill breast cancer cells that overexpress HER-2. We also defined an association between HER-2 and P-Akt in primary breast tissues, suggesting that these inhibitors may benefit patients in need of new treatment options.
MicroRNA profiling of the murine hematopoietic system
Authors: Monticelli Silvia , Ansel K Mark , Xiao Changchun , Socci Nicholas D , Krichevsky Anna M , Thai To-Ha , Rajewsky Nikolaus , Marks Debora S , Sander Chris , Rajewsky Klaus , Rao Anjana , Kosik Kenneth S 
Published: August 2005

Abstract
Background
MicroRNAs (miRNAs) are a class of recently discovered noncoding RNA genes that post-transcriptionally regulate gene expression. It is becoming clear that miRNAs play an important role in the regulation of gene expression during development. However, in mammals, expression data are principally based on whole tissue analysis and are still very incomplete.

Results
We used oligonucleotide arrays to analyze miRNA expression in the murine hematopoietic system. Complementary oligonucleotides capable of hybridizing to 181 miRNAs were immobilized on a membrane and probed with radiolabeled RNA derived from low molecular weight fractions of total RNA from several different hematopoietic and neuronal cells. This method allowed us to analyze cell type-specific patterns of miRNA expression and to identify miRNAs that might be important for cell lineage specification and/or cell effector functions.

Conclusion
This is the first report of systematic miRNA gene profiling in cells of the hematopoietic system. As expected, miRNA expression patterns were very different between hematopoietic and non-hematopoietic cells, with further subtle differences observed within the hematopoietic group. Interestingly, the most pronounced similarities were observed among fully differentiated effector cells (Th1 and Th2 lymphocytes and mast cells) and precursors at comparable stages of differentiation (double negative thymocytes and pro-B cells), suggesting that in addition to regulating the process of commitment to particular cellular lineages, miRNAs might have an important general role in the mechanism of cell differentiation and maintenance of cell identity.
Functional FGFR4 Gly388Arg Polymorphism Predicts Prognosis in Lung Adenocarcinoma Patients
Authors: Spinola Monica , Leoni Vera , Pignatiello Carmen , Conti Barbara , Ravagnani Fernando , Pastorino Ugo , Dragani Tommaso A. 
Published: August 2005

Purpose Fibroblast growth factor receptor 4 (FGFR4) is a member of a family of transmembrane receptors with ligand-induced tyrosine kinase activity. The Gly388Arg polymorphism in the FGFR4 gene was reported to modulate cancer cell migration in vitro and to be associated with breast, colon, and prostate cancer prognostic parameters. The purpose of this study was to investigate the involvement of the FGFR4 polymorphism in lung tumorigenesis. Patients and Methods A case-control study was performed including 274 patients with histologically confirmed lung adenocarcinoma and 401 healthy control subjects from general population. mRNA expression analysis was carried out in healthy lung of cancer patients. Results Patients with the Arg/Arg or Gly/Arg genotype compared to those with a Gly/Gly genotype had an earlier age at cancer onset (median age, 60.2 v 63.4 years), higher proportion of poor clinical stage disease (hazard ratio [HR], 2.3; 95% CI, 1.4 to 3.9; P = .002), of nodal involvement (HR, 1.9; 95% CI, 1.1 to 3.2; P = .027), or of short-term survivors (HR, 1.6; 95% CI, 1.1 to 2.3; P = .008). In healthy lungs, FGFR4 did not show allele-specific expression and mRNA levels were not associated with genotype. Conclusion This study suggests that FGFR4 Gly388Arg polymorphism may predict prognosis in lung adenocarcinoma.
Promoter Polymorphism of the CD14 Endotoxin Receptor Gene Is Associated With Biliary Atresia and Idiopathic Neonatal Cholestasis
Authors: Shih Hsiang-Hung , Lin Tsun-Mei , Chuang Jiin-Haur , Eng Hock-Liew , Juo Suh-Hang Hank , Huang Fu-Chen , Chen Chao-Long , Chen Huey-Ling 
Published: August 2005

Objective.To investigate whether single-nucleotide polymorphisms in the promoter regions of endotoxin-responsive genes CD14 and tumor necrosis factor-α (TNF-α) are associated with biliary atresia (BA) and idiopathic neonatal cholestasis (INC).Methods.We obtained genomic DNA from 90 patients with established diagnosis of BA and 28 patients with INC. Forty-two adult patients with hepatitis B–related cirrhosis and 143 healthy children served as control populations. The genotypes of CD14/C(−159)T and TNF-α/G(−308)A (G allele, TNF*1; A allele, TNF*2) were determined by using a restriction enzyme–based assay. Plasma soluble CD14 levels were determined in different disease stages and genotypes of BA.Results.The frequencies of T allele and T/T homozygosity of the CD14/−159 promoter polymorphism were significantly higher in patients with BA (T allele: 61.7%; T/T genotype: 42.2%) and in patients with INC (T allele: 67.9%; T/T genotype: 53.6%) but not in control populations. Decrease of plasma soluble CD14 from the early stage of BA when the patients received a Kasai operation to the late stage of liver cirrhosis was observed in carriers of the T/T and T/C genotypes but not in carriers of the C/C genotype. The TNF-α/−308 promoter polymorphisms (TNF*1 and TNF*2) were not associated with BA.Conclusion.These findings show that the single-nucleotide polymorphism at CD14/−159 is associated with the development of BA and INC. Endotoxin susceptibility may play a role in the pathogenesis of infantile cholestasis.