bluuebunny/crossref_metadata_2025_split huggingface dataset:
Entrusting life to professionals: A phenomenological hermeneutical study of older persons' participation in prehospital emergency care involving municipal home care and ambulance services
Authors: Hjalmarsson Anna , Östlund Gunnel , Asp Margareta , Kerstis Birgitta , Holmberg Mats 
Published: November 2023

AbstractBackgroundParticipation in care is considered to promote safe and qualitative care. Care‐dependent older persons ageing in place have increased emergency care needs, which initiate inter‐organisational collaboration involving municipal home care and ambulance services. Previous research concludes that uncertainties exist regarding what participation in care means in clinical practice, which necessitates the need to illuminate the phenomenon for older persons in critical life situations.AimThis study aimed to illuminate meanings of participation in prehospital emergency care from the perspective of care‐dependent older persons experiencing acute illness at home.DesignThis study has a qualitative design with a lifeworld approach.MethodA phenomenological hermeneutical method was used to analyse transcribed telephone interviews with eleven care‐dependent persons aged 70–93 years.ResultsCare‐dependent older persons' participation in prehospital emergency care means ‘Entrusting life to professional caregivers’ when being in helpless solitude and existentially unsafe, which emphasises a deepened interpersonal dependence. Meanings of participation in care from the perspective of older persons involve Being reassured in togetherness, Being pliant in trust of emergency expertise, Being enabled through the agency of professional caregivers, and Encountering readiness in the emergency care chain.ConclusionCare‐dependent older persons' participation in prehospital emergency care is existential and involves interpersonal dependence. Togetherness brings reassurance, safety and opportunity for emotional rest while accessing the professional caregivers' power, competence and abilities which provide opportunities for existence and movement towards well‐being and continued living.Implications for practicePrehospital emergency care from the perspective of care‐dependent older persons transcends organisational boundaries and includes the municipal mobile safety alarm service. The involved municipal and regional organisations need to provide support by implementing lifeworld‐led care models and care alternatives that enable professionals to recognise the existential dimension of participation in care.
Characterizing the Suture Pullout Force for Human Small Bowel
Authors: Gong Alex T. , Yau Shi-Wen Olivia , Erickson Hans B. , Toepfer Rudolph J. , Zhang Jessica , Deschmidt Aleah M. , Parsey Conner J. , Norfleet Jack E. , Sweet Robert M. 
Published: November 2023

Abstract
Performing a small bowel anastomosis, or reconnecting small bowel segments, remains a core competency and critical step for the successful surgical management of numerous bowel and urinary conditions. As surgical education and technology moves toward improving patient outcomes through automation and increasing training opportunities, a detailed characterization of the interventional biomechanical properties of the human bowel is important. This is especially true due to the prevalence of anastomotic leakage as a frequent (3.02%) postoperative complication of small bowel anastomoses. This study aims to characterize the forces required for a suture to tear through human small bowel (suture pullout force, SPOF), while analyzing how these forces are affected by tissue orientation, suture material, suture size, and donor demographics. 803 tests were performed on 35 human small bowel specimens. A uni-axial test frame was used to tension sutures looped through 10 × 20 mm rectangular bowel samples to tissue failure. The mean SPOF of the small bowel was 4.62±1.40 N. We found no significant effect of tissue orientation (p = 0.083), suture material (p = 0.681), suture size (p = 0.131), age (p = 0.158), sex (p = .083), or body mass index (BMI) (p = 0.100) on SPOF. To our knowledge, this is the first study reporting human small bowel SPOF. Little research has been published about procedure-specific data on human small bowel. Filling this gap in research will inform the design of more accurate human bowel synthetic models and provide an accurate baseline for training and clinical applications.
Motion Reconstruction of Fast-Rotating Rigid Bodies
Authors: Neurauter Rene , Holzinger Stefan , Neuhauser Michael , Fischer Jan-Thomas , Gerstmayr Johannes 
Published: November 2023

Abstract
Motion reconstruction and navigation require accurate orientation estimation. Modern orientation estimation methods utilize filtering algorithms, such as the Kalman filter or Madgwick's algorithm. However, these methods do not address potential sensor saturation, which may occur within short time periods in highly dynamic applications, such as, e.g., particle tracking in snow avalanches, leading to inaccurate orientation estimates. In this paper, we present two algorithms for orientation estimation combining magnetometer and partially saturated gyrometer readings. One algorithm incorporates magnetic field vector observations and the full nonlinearity of the exponential map. The other, computationally more efficient algorithm builds on a linearization of the exponential map and is solved analytically. Both algorithms are then applied to measurement data from four different experiments, with two of them being snow avalanche experiments. Moreover, Madgwick's filtering algorithm was used to validate the proposed algorithms. The two algorithms improved the orientation estimation significantly in all experiments. Hence, the proposed algorithms can improve the performance of existing sensor fusion algorithms significantly.
Design Synthesis of a 4D-Printed Self-Tying Knot With Programmable Morphology
Authors: Bhattacharyya Anurag , Kim Jinyoung , Alacoque Lee R. , James Kai A. 
Published: November 2023

Abstract
Smart materials provide a means by which we can create engineered mechanisms that artificially mimic the adaptability, flexibility, and responsiveness found in biological systems. Previous studies have developed material-based actuators that could produce targeted shape changes. Here, we extend this capability by introducing a novel computational and experimental method for design and synthesis of a material-based mechanism capable of achieving complex pre-programmed motion. By combining active and passive materials, the algorithm can encode the desired movement into the material distribution of the mechanism. We use multimaterial, multiphysics topology optimization to design a set of kinematic elements that exhibit basic bending and torsional deflection modes. We then use a genetic algorithm to optimally arrange these elements into a sequence that produces the desired motion. We also use experimental measurements to accurately characterize the angular deflection of the 3D-printed kinematic elements in response to thermomechanical loading. We demonstrate this new capability by de novo design of a 3D-printed self-tying knot. This method advances a new paradigm in mechanism design that could enable a new generation of material-driven machines that are lightweight, adaptable, robust to damage, and easily manufacturable by 3D printing.
Towards Sustainable Integration: Techno-Economic Analysis and Future Perspectives of Co-Located Wind and Hydrogen Energy Systems
Authors: Li Honglin , Zhang Jie 
Published: November 2023

Abstract
This article presents a comprehensive study that focuses on the techno-economic analysis of co-located wind and hydrogen energy integration within an integrated energy system (IES). The research investigates four distinct cases, each exploring various configurations of wind farms, electrolyzers, batteries, hydrogen storage tanks, and fuel cells. To obtain optimal results, the study employs a sophisticated mathematical optimization model formulated as a mixed-integer linear program. This model helps determine the most suitable component sizes and hourly energy scheduling patterns. The research utilizes historical meteorological data and wholesale market prices from diverse regions as inputs, enhancing the study’s applicability and relevance across different geographical locations. Moreover, sensitivity analyses are conducted to assess the impact of hydrogen prices, regional wind profiles, and potential future fluctuations in component prices. These analyses provide valuable insights into the robustness and flexibility of the proposed IES configurations under varying market conditions and uncertainties. The findings reveal cost-effective system configurations, strategic component selections, and implications of future energy scenarios. Specifically comparing to configurations that only have wind and battery combinations, we find that incorporating an electrolyzer results in a 7% reduction in the total cost of the IES, and utilizing hydrogen as the storage medium for fuel cells leads to a 26% cost reduction. Additionally, the IES with hybrid hydrogen and battery energy storage achieves even higher and stable power output. This research facilitates decision-making, risk mitigation, and optimized investment strategies, fostering sustainable planning for a resilient and environmentally friendly energy future.
Direct Numerical Simulation of Transitional and Turbulent Flows Over Multi-Scale Surface Roughness—Part II: The Effect of Roughness on the Performance of a High-Pressure Turbine Blade
Authors: Nardini Massimiliano , Jelly Thomas O. , Kozul Melissa , Sandberg Richard D. , Vitt Paul , Sluyter Greg 
Published: November 2023

Abstract
Turbine blades generally present surface roughness introduced in the manufacturing process or caused by in-service degradation, which can have a significant impact on aero-thermal performance. A better understanding of the fundamental physical mechanisms arising from the interaction between the roughness and the turbine flow at engine-relevant conditions can provide insights for the design of blades with improved efficiency and longer operational life. To this end, a high-fidelity numerical framework combining a well-validated solver for direct numerical simulation and a second-order accurate immersed boundary method is employed to predict roughness-induced aero-thermal effects on an LS89 high-pressure turbine (HPT) blade at engine-relevant conditions. Different amplitudes and distributions of surface roughness are investigated and a reference smooth-blade simulation under the same flow conditions is conducted for comparison. Roughness of increasing amplitude progressively shifts the blade suction side boundary layer transition upstream, producing larger values of the turbulent kinetic energy and higher total wake losses. The on-surface data-capturing capabilities of the numerical framework provide direct measurements of the heat flux and the skin friction coefficient, hence offering quantitative information between the surface topology and engineering-relevant performance parameters. This work may provide a benchmark for future numerical studies of turbomachinery flows with roughness.
Microbial biogeography of the eastern Yucatán carbonate aquifer
Authors: Osburn Magdalena R. , Selensky Matthew J. , Beddows Patricia A. , Jacobson Andrew , DeFranco Karyn , Merediz-Alonso Gonzalo 
Published: November 2023

ABSTRACTConstraining the spatial distribution of microorganisms and their ecological interactions is crucial for informing biogeochemistry. To that end, we explore horizontal and vertical patterns of microbial biogeography in the eastern Yucatán carbonate aquifer by examining the relative abundance of microbial taxavia16S rRNA gene sequencing. As one of the largest anchialine groundwater systems on Earth, the density-stratified Yucatán aquifer consists of a meteoric lens overlying saline groundwater. The myriad sinkholes (cenotes) of the eastern peninsula lead into a vast network of subsurface conduits. Several studies describe microbial communities within specific regions of the aquifer, yet fundamental questions remain regarding the ecology and distribution of biogeochemically relevant microbes. Our analysis demonstrates that this aquifer hosts a distinct microbiome from nearby seawater, with regionalism observed across cave systems and vertical water column zones. We apply novel software to construct taxonomic co-occurrence networks at different scales and categorize highly connected groups of taxa into potential niches. Our network analysis approach suggests that ubiquitous, metabolically flexible taxa such as the familyComamonadaceaeact as ecological linchpins across several niches, often directly or indirectly co-occurring with taxa capable of anammox (e.g.,Gemmataceae), methanotrophy (e.g.,Methyloparacoccus), or organoheterotrophy. Furthermore, communities from a deep, pit-like cenote open to the surface show the strongest niche partitioning between water column zones, differing from those encountered throughout the mostly dark and oligotrophic aquifer system, including another deep pit cenote with no direct surface opening. Our results suggest that members of a core microbiome could modulate different biogeochemical regimes depending on location, acting as reservoirs of metabolic potential in disparate environments of this groundwater system.IMPORTANCEThe extensive Yucatán carbonate aquifer, located primarily in southeastern Mexico, is pockmarked by numerous sinkholes (cenotes) that lead to a complex web of underwater caves. The aquifer hosts a diverse yet understudied microbiome throughout its highly stratified water column, which is marked by a meteoric lens floating on intruding seawater owing to the coastal proximity and high permeability of the Yucatán carbonate platform. Here, we present a biogeographic survey of bacterial and archaeal communities from the eastern Yucatán aquifer. We apply a novel network analysis software that models ecological niche space from microbial taxonomic abundance data. Our analysis reveals that the aquifer community is composed of several distinct niches that follow broader regional and hydrological patterns. This work lays the groundwork for future investigations to characterize the biogeochemical potential of the entire aquifer with other systems biology approaches.
Development of a Tag/Catcher-mediated capsid virus-like particle vaccine presenting the conserved
Neisseria gonorrhoeae
SliC antigen that blocks human lysozyme
Authors: Martinez Fabian G. , Zielke Ryszard A. , Fougeroux Cyrielle E. , Li Lixin , Sander Adam F. , Sikora Aleksandra E. 
Published: November 2023

ABSTRACT

Virus-like particles (VLPs) are promising nanotools for the development of subunit vaccines due to high immunogenicity and safety. Herein, we explored the versatile and effective Tag/Catcher-AP205 capsid VLP (cVLP) vaccine platform to address the urgent need for the development of an effective and safe vaccine against gonorrhea. The benefits of this clinically validated cVLP platform include its ability to facilitate unidirectional, high-density display of complex/full-length antigens through an effective split-protein Tag/Catcher conjugation system. To assess this modular approach for making cVLP vaccines, we used a conserved surface lipoprotein, SliC, that contributes to the
Neisseria gonorrhoeae
defense against human lysozyme, as a model antigen. This protein was genetically fused at the N- or C-terminus to the small peptide Tag enabling their conjugation to AP205 cVLP, displaying the complementary Catcher. We determined that SliC with the N-terminal SpyTag, N-SliC, retained lysozyme-blocking activity and could be displayed at high density on cVLPs without causing aggregation. In mice, the N-SliC-VLP vaccines, adjuvanted with AddaVax or CpG, induced significantly higher antibody titers compared to controls. In contrast, similar vaccine formulations containing monomeric SliC were non-immunogenic. Accordingly, sera from N-SliC-VLP-immunized mice also had significantly higher human complement-dependent serum bactericidal activity. Furthermore, the N-SliC-VLP vaccines administered subcutaneously with an intranasal boost elicited systemic and vaginal IgG and IgA, whereas subcutaneous delivery alone failed to induce vaginal IgA. The N-SliC-VLP with CpG (10 µg/dose) induced the most significant increase in total serum IgG and IgG3 titers, vaginal IgG and IgA, and bactericidal antibodies.
Adenovirus E1B-55K controls SUMO-dependent degradation of antiviral cellular restriction factors
Authors: Ip Wing-Hang , Tatham Michael H. , Krohne Steewen , Gruhne Julia , Melling Michael , Meyer Tina , Gornott Britta , Bertzbach Luca D. , Hay Ronald T. , Rodriguez Estefania , Dobner Thomas 
Published: November 2023

ABSTRACT
The human adenovirus species C type 5 (HAdV-C5) early region 1B 55 kDa (E1B-55K) protein is a multifunctional protein that promotes viral replication and adenovirus-mediated cell transformation through various mechanisms that primarily counteract host intrinsic and innate immunity. These include post-translational activities that exploit the host cell ubiquitin and small ubiquitin-like modifier (SUMO) conjugation machineries to regulate antiviral cellular restriction factors. However, despite significant advancements in this field, several underlying mechanisms governing these processes remain unidentified to date. Here, we performed stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative SUMO proteomics to better understand cellular consequences of E1B-55K-mediated host cell modulation and adenovirus infection in general. We assessed cellular proteins for abundance changes and SUMO2 conjugate proteome changes during infection with wild-type HAdV-C5 or an E1B-55K deletion mutant. We provide evidence that changes in the SUMOylated proteome have the potential to regulate the DNA damage response, cell cycle control, chromatin assembly, and gene transcription and present these data as a resource for the research community. Strikingly, we identified a SUMO-dependent, ubiquitin-mediated degradation mechanism for some SUMO substrates, suggesting that E1B-55K may use multiple mechanisms to alter the activity of restrictive cellular pathways.

IMPORTANCE
Human adenoviruses (HAdVs) generally cause mild and self-limiting diseases of the upper respiratory and gastrointestinal tracts but pose a serious risk to immunocompromised patients and children. Moreover, they are widely used as vectors for vaccines and vector-based gene therapy approaches. It is therefore vital to thoroughly characterize HAdV gene products and especially HAdV virulence factors. Early region 1B 55 kDa protein (E1B-55K) is a multifunctional HAdV-encoded oncoprotein involved in various viral and cellular pathways that promote viral replication and cell transformation. We analyzed the E1B-55K dependency of SUMOylation, a post-translational protein modification, in infected cells using quantitative proteomics. We found that HAdV increases overall cellular SUMOylation and that this increased SUMOylation can target antiviral cellular pathways that impact HAdV replication. Moreover, we showed that E1B-55K orchestrates the SUMO-dependent degradation of certain cellular antiviral factors. These results once more emphasize the key role of E1B-55K in the regulation of viral and cellular proteins in productive HAdV infections.
Characterization of non-neutralizing human monoclonal antibodies that target the M1 and NP of influenza A viruses
Authors: Rijnink Willemijn Frederique , Stadlbauer Daniel , Puente-Massaguer Eduard , Okba Nisreen M. A. , Kirkpatrick Roubidoux Ericka , Strohmeier Shirin , Mudd Philip A. , Schmitz Aaron , Ellebedy Ali , McMahon Meagan , Krammer Florian 
Published: November 2023

ABSTRACT
Improved broad-spectrum influenza virus vaccines are desperately needed to provide protection against both drifted seasonal and emerging pandemic influenza A viruses (IAVs). Antibody-based protection from influenza A virus-induced morbidity and mortality is traditionally associated with neutralizing antibodies. As such, vaccine efforts have solely focused on the hemagglutinin (HA) as a vaccine target; however, the HA is mutation prone resulting in the need for annual vaccine reformulation. Broad-spectrum vaccines could be achieved through non-neutralizing antibodies that target conserved influenza virus antigens. Here, we describe six human monoclonal antibodies (mAbs) isolated from two H3N2-infected donors that showed robust binding against the conserved internal nucleoprotein (NP) or matrix protein 1 (M1) of IAV strains. Despite the capacity for potent antigen binding, substantial morbidity was observed in mice prophylactically treated with these mAbs and then challenged with A/Netherlands/602/2009 (H1N1) or A/Switzerland/9715293/2013 (H3N2) viruses. While our findings need to be confirmed with a larger number of mAbs and with polyclonal serum, these findings suggest that human NP and M1 antibodies that are elicited following IAV infection/vaccination do not protect from substantial weight loss in the mouse model and imply that protection afforded targeting these antigens following vaccination/infection is most likely the result of cellular-based immunity.

IMPORTANCE
Currently, many groups are focusing on isolating both neutralizing and non-neutralizing antibodies to the mutation-prone hemagglutinin as a tool to treat or prevent influenza virus infection. Less is known about the level of protection induced by non-neutralizing antibodies that target conserved internal influenza virus proteins. Such non-neutralizing antibodies could provide an alternative pathway to induce broad cross-reactive protection against multiple influenza virus serotypes and subtypes by partially overcoming influenza virus escape mediated by antigenic drift and shift. Accordingly, more information about the level of protection and potential mechanism(s) of action of non-neutralizing antibodies targeting internal influenza virus proteins could be useful for the design of broadly protective and universal influenza virus vaccines.