bluuebunny/crossref_metadata_2025_split huggingface dataset:
Particle tracking in snow avalanches with in situ calibrated inertial measurement units
Authors: Winkler Robert , Neuhauser Michael , Neurauter Rene , Erlacher Felix , Steinkogler Walter , Fischer Jan-Thomas 
Published: January 2024

Abstract
In the course of an artificially triggered avalanche, a particle tracking procedure is combined with supplementary measurements, including Global Navigation Satellite System (GNSS) positioning, terrestrial laser scanning and Doppler radar measurements. Specifically, an intertial measurement unit is mounted inside a rigid sphere, which is placed in the avalanche track. The sphere is entrained by the moving snow, recording translational accelerations, angular velocities and the flux density of Earth's magnetic field. Based on the recorded data, we present a threefold analysis: (i) a qualitative data interpretation, identifying different particle motion phases which are associated with corresponding flow regimes, (ii) a quantitative time integration algorithm, determining the corresponding particle trajectory and associated velocities on the basis of standard sensor calibration, and (iii) an improved quantitative evaluation relying on a novel in situ sensor calibration technique, which is motivated by the limitations of the given dataset. The final results, i.e. the evolution of the angular orientation of the sensor unit, translational and rotational velocities and estimates of the sensor trajectory, are assessed with respect to their reliability and relevance for avalanche dynamics as well as for future design of experiments.
Greater than the sum of its parts: optical remote sensing and sediment core data provide a holistic perspective on glacial processes
Authors: Gage Henry Jacob Miller , Eyles Carolyn Hope 
Published: January 2024

Abstract
In this letter we make the case that closer integration of sediment core and passive optical remote sensing data would provide new insights into past and contemporary glacio-sedimentary processes. Sediment cores are frequently used to study past glacial processes and environments as they contain a lengthy geochemical and sedimentological record of changing conditions. In contrast, optical remote sensing imagery is used extensively to examine contemporary glacial processes, including meltwater dynamics, glacial retreat, calving, and ice accumulation. While paleoenvironmental data from sediment cores and optical remote sensing imagery are rarely used in tandem, they are complementary. Sediment core records are spatially discrete, providing long-term paleoenvironmental proxy data which require assumptions about environment-sediment linkages. Optical imagery offers precise, spatially extensive data to visualize contemporary processes often limited in their temporal extent. We suggest that methodologies which integrate optical remotely sensing with sediment core data allow direct observation of processes interpolated from sedimentological analysis and achieve a more holistic perspective on glacial processes. This integration addresses the limitations of both data sources and can achieve a stronger understanding of glacier dynamics by expanding the spatiotemporal extent of data, reducing the uncertainty of interpretations, and broadening the local analyses to regional and global scales.
Introduction of hospital-based health technology assessment in China: experiences from seven pilot hospitals
Authors: Lyu Lanting , Shi Wenkai , Kidholm Kristian , Bai Fei , Lin Xia , Fu Jinlan , Li Tianqing , Li Guoxun , Luo Li , Wang Ting , Yang Hai 
Published: January 2024

Abstract

Objectives
This study aimed to introduce a pilot program for hospital-based health technology assessment (HB-HTA) in China and present the participants’ experiences based on seven case studies from seven tertiary hospitals.

Methods
One-year pilot projects were initiated at the beginning of 2018. Seven pilot hospitals were closely followed from the beginning until the completion of their pilot HTA project. Regular interviews were conducted with the hospital managers leading the HB-HTA projects and key members of the special HTA teams. Observations were made based on field trips and written HTA reports.

Results
Three pilot projects evaluated the use of medical consumables, three evaluated the use of surgical or medical interventions, and one evaluated an innovative management model for ventilators. Real-world data were collected from all the pilot projects to assist with the assessments. Most HB-HTA pilot projects achieved remarkable results such as improvements in economic efficiency; however, there were also obvious deficiencies such as the lack of a necessary cost-effectiveness analysis.

Conclusions
The results varied among the seven HB-HTA pilot projects. The HB-HTA pilot program was implemented to promote the use of HB-HTA in hospital decision making in China. At the same time, HB-HTA in China faces challenges. We have made some policy recommendations based on the findings of the pilot projects.
Environmental obligations of business entities during armed conflicts
Authors: Traoré Sâ Benjamin , Cirimwami Ezéchiel Amani 
Published: January 2024

AbstractInternational humanitarian law (IHL) does not address business entities, except in situations where they directly participate in hostilities, and there is no reference to business actors in the International Committee of the Red Cross's recent Guidelines on the Protection of the Natural Environment in Armed Conflict. Yet, there has been an increasing reaffirmation of specific “duties”, “obligations” or “responsibilities” imposed on private companies operating in conflict zones. For instance, the UN Guiding Principles on Business and Human Rights suggest that business entities should respect IHL rules in addition to human rights during armed conflicts, and the third revised draft of the international legally binding instrument on transnational corporations and other business enterprises refers to IHL as an interpretative framework of human rights obligations of States and businesses. The International Law Commission's 2022 Draft Principles on the Protection of the Environment during Armed Conflicts are even more specific, providing that corporations should exercise due diligence concerning the protection of the environment when acting in conflict-affected areas. However, these references to IHL as applicable to business activities remain vague and lack elaboration. This paper intends to close this gap by clarifying whether and, if so, the extent to which IHL imposes environmental obligations upon private companies in conflict situations. It submits that business entities bear environmental duties during armed conflicts deriving from IHL rules and other complementary sources of international law. The paper further discusses the content of the obligation of business entities not to harm the environment as well as their due diligence obligation.
The efficacy of different torque profiles for weight compensation of the hand
Authors: van der Burgh Bas J. , Filius Suzanne J. , Radaelli Giuseppe , Harlaar Jaap 
Published: January 2024

Abstract
Orthotic wrist supports will be beneficial for people with muscular weakness to keep their hand in a neutral rest position and prevent potential wrist contractures. Compensating the weight of the hands is complex since the level of support depends on both wrist and forearm orientations. To explore simplified approaches, two different weight compensation strategies (constant and linear) were compared to the theoretical ideal sinusoidal profile and no compensation in eight healthy subjects using a mechanical wrist support system. All three compensation strategies showed a significant reduction of 47–53% surface electromyography activity in the anti-gravity m. extensor carpi radialis. However, for the higher palmar flexion region, a significant increase of 44–61% in the m. flexor carpi radialis was found for all compensation strategies. No significant differences were observed between the various compensation strategies. Two conclusions can be drawn: (1) a simplified torque profile (e.g., constant or linear) for weight compensation can be considered as equally effective as the theoretically ideal sinusoidal profile and (2) even the theoretically ideal profile provides no perfect support as other factors than weight, such as passive joint impedance, most likely influence the required compensation torque for the wrist joint.
Un violador en tu camino: An artistic intervention against patriarchal injustice in Chile
Authors: PINZAUTI GIORGIA 
Published: January 2024

AbstractIn 2019, Chile’s artistic collective Las Tesis staged the viral feminist performance “Un violador en tu camino” (A Rapist in Your Path), as a popular protest against Sebastián Piñera’s government. This article analyzes the geopolitical context behind Las Tesis, focusing on Chile’s 2019 events and the motivations behind the performance in Valparaíso. Subsequently, I explore the performance’s structure and lyrics as a form of artivism, emphasizing its impact. The third section of this article will be dedicated to exploring the linguistic and musical adaptations of the same performance undertaken by various women’s collectives, associations, or informal groups across the globe.
N1-methylation of adenosine (m1A) in ND5 mRNA leads to complex I dysfunction in Alzheimer’s disease
Authors: Jörg Marko , Plehn Johanna E. , Kristen Marco , Lander Marc , Walz Lukas , Lietz Christine , Wijns Julie , Pichot Florian , Rojas-Charry Liliana , Wirtz Martin Katja M. , Ruffini Nicolas , Kreim Nastasja , Gerber Susanne , Motorin Yuri , Endres Kristina , Rossmanith Walter , Methner Axel , Helm Mark , Friedland Kristina 
Published: January 2024

AbstractOne mechanism of particular interest to regulate mRNA fate post-transcriptionally is mRNA modification. Especially the extent of m1A mRNA methylation is highly discussed due to methodological differences. However, one single m1A site in mitochondrial ND5 mRNA was unanimously reported by different groups. ND5 is a subunit of complex I of the respiratory chain. It is considered essential for the coupling of oxidation and proton transport. Here we demonstrate that this m1A site might be involved in the pathophysiology of Alzheimer’s disease (AD). One of the pathological hallmarks of this neurodegenerative disease is mitochondrial dysfunction, mainly induced by Amyloid β (Aβ). Aβ mainly disturbs functions of complex I and IV of the respiratory chain. However, the molecular mechanism of complex I dysfunction is still not fully understood. We found enhanced m1A methylation of ND5 mRNA in an AD cell model as well as in AD patients. Formation of this m1A methylation is catalyzed by increased TRMT10C protein levels, leading to translation repression of ND5. As a consequence, here demonstrated for the first time, TRMT10C induced m1A methylation of ND5 mRNA leads to mitochondrial dysfunction. Our findings suggest that this newly identified mechanism might be involved in Aβ-induced mitochondrial dysfunction.
Deletion of the prorenin receptor in the ureteric bud in mice inhibits Dot1/H3K79 pathway
Authors: Song Renfang , Yosypiv Ihor V. 
Published: January 2024

Abstract
Background
The prorenin receptor (PRR) plays a critical role in ureteric bud (UB) branching morphogenesis. DOT1 Like (DOT1L), a histone methyltransferase specific for Histone 3 lysine 79 (H3K79), is important for differentiation of the UB-derived renal collecting duct cells. In this study, we tested whether DOT1L/H3 dimethyl K79 (H3m2K79) are regulated by PRR deletion in the UB and UB-derived collecting ducts in the embryonic mouse kidneys.

Methods
Mutant Hoxb7Cre+/PRRflox/flox (PRRUB-/-) and control PRRUB+/+, mice were studied on embryonic (E) day E17.5. DOT1L mRNA and protein expression in the kidney was examined by real-time qRT-PCR and immunohistochemistry, respectively. H3m2K79 protein expression was determined by immunohistochemistry and Western blot analysis.

Results
DOT1L mRNA levels were decreased in mutant compared to control mice (0.68 ± 0.06 vs. 1.0 ± 0.01, p < 0.01). DOT1L and H3m2K79 immunostaining was reduced in the mutant vs. control kidneys (Dot1: 0.62 ± 0.03 vs. 1.0 ± 0.01, p < 0.05; H3m2K79: 0.64 ± 0.04 vs.1.1 ± 0.01. p < 0.05.). Western blot analysis revealed decreased H3m2K79 protein levels in mutant compared to control kidneys (1.0 ± 0.06 vs. 1.5 ± 0.02, p < 0.05).

Conclusion
Targeted deletion of the PRR in the UB and UB-derived collecting ducts results in reduced DOT1L gene/protein and H3m2K79 protein expression in the embryonic mouse metanephroi in vivo.

Impact

The role of histone methylation in mediating the effect of the prorenin receptor on the ureteric bud branching (UB) morphogenesis and urine acidification during kidney development is unknown.

We demonstrate that histone H3 lysine (K) 79 dimethylation by methyltransferase Dot1 is reduced in the embryonic kidney of mice that lack the prorenin receptor in the UB lineage.
The Swiss Spinal Cord Injury Cohort Study (SwiSCI) biobank: from concept to reality
Authors: Zeh Ramona M. , Glisic Marija , Capossela Simona , Bertolo Alessandro , Valido Ezra , Jordan Xavier , Hund-Georgiadis Margret , Pannek Jürgen , Eriks-Hoogland Inge , Stucki Gerold , Stoyanov Jivko 
Published: January 2024

Abstract
Objectives
To describe the concept, establishment and the operationalization of the biobank of the Swiss Spinal Cord Injury Cohort Study (SwiSCI), the available biosamples, and demographic and clinical characteristics of study participants.

Setting
The SwiSCI biobank is a platform for research within SwiSCI. It collects and processes serum, plasma, PBMCs, RNA, DNA, and urine from three rehabilitation centers. Samples are collected at admission to first rehabilitation and at discharge. Additionly, the biobank provides services to projects nested in SwiSCI or otherclinical trials among Spinal Cord Injury population.

Methods
Descriptive statistics were used for an overview of available biosamples, study participant characteristics, and comparison of the participating centers.

Results
Between the SwiSCI biobank establishment on June 27th, 2016, and October 19th, 2023, the SwiSCI Study has obtained informed consent from 524 individuals. Of these, 315 (60.1%) have agreed to donate biospecimens to the biobank. The average age of the contributors was 54 years (range: 38–65), with the majority being male (80%). Most participants suffered from traumatic injuries (66%) and were classified as paraplegic (64%). Approximately 80% presented with motor and sensory-incomplete SCI. The median Spinal Cord Independence Measure (SCIM) score was 31 (Interquartile Range: 19–58). The proportion of individuals providing paired biosamples at two distinct time points ranged from 63% (for RNA) to 65% (for urine and urine sediment).

Conclusions
The SwiSCI biobank is a unique platform designed to serve as a basis for collaborative SCI research, including multi-omics approaches. The longitudinal collection of biospecimens and cryopreservation of multiple aliquots for each participant are fundamental for scrutinizing the temporal associations, ensuring research reproducibility, and achieving an adequate sample size for future investigations.
Inhibition of Aurora B kinase (AURKB) enhances the effectiveness of 5-fluorouracil chemotherapy against colorectal cancer cells
Authors: Shah Esha T. , Molloy Christopher , Gough Madeline , Kryza Thomas , Samuel Selwin G. , Tucker Amos , Bhatia Maneet , Ferguson Genevieve , Heyman Rebecca , Vora Shivam , Monkman James , Bolderson Emma , Kulasinghe Arutha , He Yaowu , Gabrielli Brian , Hooper John D. , Richard Derek J. , O’Byrne Kenneth J. , Adams Mark N. 
Published: January 2024

AbstractBackground5-Fluorouracil (5-FU) remains a core component of systemic therapy for colorectal cancer (CRC). However, response rates remain low, and development of therapy resistance is a primary issue. Combinatorial strategies employing a second agent to augment the therapeutic effect of chemotherapy is predicted to reduce the incidence of treatment resistance and increase the durability of response to therapy.MethodsHere, we employed quantitative proteomics approaches to identify novel druggable proteins and molecular pathways that are deregulated in response to 5-FU, which might serve as targets to improve sensitivity to chemotherapy. Drug combinations were evaluated using 2D and 3D CRC cell line models and an ex vivo culture model of a patient-derived tumour.ResultsQuantitative proteomics identified upregulation of the mitosis-associated protein Aurora B (AURKB), within a network of upregulated proteins, in response to a 24 h 5-FU treatment. In CRC cell lines, AURKB inhibition with the dihydrogen phosphate prodrug AZD1152, markedly improved the potency of 5-FU in 2D and 3D in vitro CRC models. Sequential treatment with 5-FU then AZD1152 also enhanced the response of a patient-derived CRC cells to 5-FU in ex vivo cultures.ConclusionsAURKB inhibition may be a rational approach to augment the effectiveness of 5-FU chemotherapy in CRC.
Deubiquitinase USP7 stabilizes KDM5B and promotes tumor progression and cisplatin resistance in nasopharyngeal carcinoma through the ZBTB16/TOP2A axis
Authors: Zhang Bin , Li Jie , Wang Yijun , Liu Xixi , Yang Xiao , Liao Zhiyun , Deng Suke , Deng Yue , Zhou Zhiyuan , Tian Yu , Wei Wenwen , Meng Jingshu , Hu Yan , Wan Chao , Zhang Zhanjie , Huang Fang , Wen Lu , Wu Bian , Sun Yajie , Li Yan , Yang Kunyu 
Published: January 2024

AbstractCisplatin-based chemotherapy improves the control of distant metastases in patients with nasopharyngeal carcinoma (NPC); however, around 30% of patients fail treatment due to acquired drug resistance. Epigenetic regulation is known to contribute to cisplatin resistance; nevertheless, the underlying mechanisms remain poorly understood. Here, we showed that lysine-specific demethylase 5B (KDM5B) was overexpressed and correlates with tumor progression and cisplatin resistance in patients with NPC. We also showed that specific inhibition of KDM5B impaired the progression of NPC and reverses cisplatin resistance, both in vitro and in vivo. Moreover, we found that KDM5B inhibited the expression of ZBTB16 by directly reducing H3K4me3 at the ZBTB16 promoter, which subsequently increased the expression of Topoisomerase II- α (TOP2A) to confer cisplatin resistance in NPC. In addition, we showed that the deubiquitinase USP7 was critical for deubiquitinating and stabilizing KDM5B. More importantly, the deletion of USP7 increased sensitivity to cisplatin by disrupting the stability of KDM5B in NPC cells. Therefore, our findings demonstrated that USP7 stabilized KDM5B and promoted cisplatin resistance through the ZBTB16/TOP2A axis, suggesting that targeting KDM5B may be a promising cisplatin-sensitization strategy in the treatment of NPC.
Repurposing proteasome inhibitors for improved treatment of triple-negative breast cancer
Authors: Larsson Peter , Pettersson Daniella , Olsson Maxim , Sarathchandra Sithumini , Abramsson Alexandra , Zetterberg Henrik , Ittner Ella , Forssell-Aronsson Eva , Kovács Anikó , Karlsson Per , Helou Khalil , Parris Toshima Z. 
Published: January 2024

AbstractTriple-negative breast cancer (TNBC) is associated with poor prognosis and limited treatment options due to the lack of important receptors (estrogen receptor [ER], progesterone receptor [PR], and human epidermal growth factor receptor 2 [HER2]) used for targeted therapy. However, high-throughput in vitro drug screening of cell lines is a powerful tool for identifying effective drugs for a disease. Here, we determine the intrinsic chemosensitivity of TNBC cell lines to proteasome inhibitors (PIs), thereby identifying potentially potent 2-drug combinations for TNBC. Eight TNBC cell lines (BT-549, CAL-148, HCC1806, HCC38, HCC70, MDA-MB-436, MDA-MB-453, and MDA-MB-468) and two controls (MCF-10A and MCF-7) were first exposed to 18 drugs (11 PIs and 7 clinically relevant chemotherapeutic agents) as monotherapy, followed by prediction of potent 2-drug combinations using the IDACombo pipeline. The synergistic effects of the 2-drug combinations were evaluated with SynergyFinder in four TNBC cell lines (CAL-148, HCC1806, HCC38, and MDA-MB-468) and three controls (BT-474, MCF-7, and T47D) in vitro, followed by further evaluation of tumor regression in zebrafish tumor models established using HCC1806 and MCF-7 cells. Monotherapy identified nine effective drugs (bortezomib, carfilzomib, cisplatin, delanzomib, docetaxel, epoxomicin, MLN-2238, MLN-9708, and nedaplatin) across all cell lines. PIs (e.g., bortezomib, delanzomib, and epoxomicin) were highly potent drugs in TNBC cells, of which bortezomib and delanzomib inhibited the chymotrypsin-like activity of the 20 S proteasome by 100% at 10 µM. Moreover, several potent 2-drug combinations (e.g., bortezomib+nedaplatin and epoxomicin+epirubicin) that killed virtually 100% of cells were also identified. Although HCC1806- and MCF-7-derived xenografts treated with bortezomib+nedaplatin and carboplatin+paclitaxel were smaller, HCC1806 cells frequently metastasized to the trunk region. Taken together, we show that PIs used in combination with platinum agents or topoisomerase inhibitors exhibit increased efficiency with almost 100% inhibition in TNBC cell lines, indicating that PIs are therefore promising compounds to use as combination therapy for TNBC.
Expression of E-cadherin by CD8+ T cells promotes their invasion into biliary epithelial cells
Authors: Davies Scott P. , Ronca Vincenzo , Wootton Grace E. , Krajewska Natalia M. , Bozward Amber G. , Fiancette Rémi , Patten Daniel A. , Yankouskaya Katharina , Reynolds Gary M. , Pat Sofia , Osei-Bordom Daniel C. , Richardson Naomi , Grover Liam M. , Weston Christopher J. , Oo Ye H. 
Published: January 2024

AbstractThe presence of CD8+ T cells in the cytoplasm of biliary epithelial cells (BEC) has been correlated with biliary damage associated with primary biliary cholangitis (PBC). Here, we characterise the mechanism of CD8+ T cell invasion into BEC. CD8+ T cells observed within BEC were large, eccentric, and expressed E-cadherin, CD103 and CD69. They were also not contained within secondary vesicles. Internalisation required cytoskeletal rearrangements which facilitated contact with BEC. Internalised CD8+ T cells were observed in both non-cirrhotic and cirrhotic diseased liver tissues but enriched in PBC patients, both during active disease and at the time of transplantation. E-cadherin expression by CD8+ T cells correlated with frequency of internalisation of these cells into BEC. E-cadherin+ CD8+ T cells formed β-catenin-associated interactions with BEC, were larger than E-cadherin- CD8+ T cells and invaded into BEC more frequently. Overall, we unveil a distinct cell-in-cell structure process in the liver detailing the invasion of E-cadherin+ CD103+ CD69+ CD8+ T cells into BEC.