bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation of novel organic‐inorganic nanoporous membranes
Authors: Sasaki Takafumi , Nagaoka Shoji , Tezuka Teppei , Suzuki Yoshiaki , Iwaki Masaya , Kawakami Hiroyoshi 
Published: July 2005

AbstractIn this study, the water permeability, the rejection property of sucrose and glucose, the fouling property of humic acid as the foulant for a novel porous fluorinated polyimide membrane made by combining the ion irradiation and plasma treatment have been reported. First, an asymmetric polyimide membrane with a defect‐free and thin skin layer was prepared, then ions on the skin layer were irradiated and the ion‐irradiated layer was treated by plasma to form nanopores in the layer. The asymmetric polyimide membranes with a defect‐free skin layer were irradiated with 50 keV He+ at 1 × 1015 ions/cm2, and the irradiated polyimide surfaces were treated by Ar glow discharge. The porous polyimide membrane showed a high water flux and excellent rejection properties and fouling resistance when compared with NTR‐7250, which is commercially available. These findings indicated that the pore size formed on the porous polyimide membrane was effectively controlled by the plasma treatment time and the skin layer thickness. Copyright © 2005 John Wiley & Sons, Ltd.
Cobaltporphyrin‐adsorbed carbon black: highly efficient electrocatalysts for oxygen reduction
Authors: Yuasa Makoto , Yamaguchi Aritomo , Oyaizu Kenichi , Fujito Yosuke , Kitao Mizuki , Sato Tsuyoshi 
Published: July 2005

Abstractmeso‐Substituted cobalt porphyrins adsorbed on carbon black were prepared as catalysts for the electroreduction of O2. The catalyst, which was prepared by using a homogenizer in mixing cobalt tetraethylporphyrin and carbon black, gave rise to electroreduction of O2 at a remarkably positive potential (Ep = 0.45 V versus saturated calomel electrode (SCE)) and showed a high selectively for the four‐electron reduction (n = 3.8). Electrochemical study and extended X‐ray absorption fine structure (EXAFS) analysis revealed that the adsorbed face‐to‐face dimeric aggregates of cobalt porphyrin molecules were highly efficient catalysts for electroreduction of O2. Copyright © 2005 John Wiley & Sons, Ltd.
Deuterium solid echo line shape study of poly(ethylene oxide) dynamics in a blend with poly(methyl methacrylate)
Authors: Cao Haihui , Lin Guoxing , Jones Alan A. 
Published: July 2005

AbstractDeuterium solid echo line shapes were measured on deuterated poly(ethylene oxide) (d4PEO) in a blend with protonated poly(methyl methacrylate) to characterize chain dynamics of this component in the blend. Line shapes were observed as a function of temperature from 183 to 243 K and echo delay times from 10 to 100 μs on a blend containing 20 wt % d4PEO. The line shapes and the associated relative intensities were quantitatively interpreted in terms of segmental motion and libration. The results of the interpretation are compared to an earlier study of deuterium spin‐lattice relaxation times over the temperature range of 313 to 413 K. A combined interpretation of both sets of data is developed based on bimodal distribution of correlation times that are separated by about 2 orders of magnitude in time. The faster mode is 30% of the correlation function with a stretched exponent near one while the slower mode is characterized by an exponent of 0.5. The source of the bimodal character is not revealed by the line shape and relaxation data but is consistent with the presence of two glass transition temperatures in this miscible blend and anomalous translational diffusion of diethyl ether through the blend. © 2005 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 43: 2433–2444, 2005
A mesoscopic Archimedean tiling having a new complexity in an ABC star polymer
Authors: Takano A. , Kawashima W. , Noro A. , Isono Y. , Tanaka N. , Dotera T. , Matsushita Y. 
Published: July 2005

AbstractThe Archimedean tiling (32.4.3.4) is a regular but complex polygonal assembly of equilateral triangles and squares. This tiling pattern with mesoscopic repeating distance has been found for an ABC star‐branched three‐component polymer composed of polyisoprene, polystyrene, and poly(2‐vinylpyridine). In this structure the environment of a molecule splits into multiple sites and two microdomains with different sizes and shapes are formed for one component. This complexity is the first observation in complex polymer systems and can lead to a new type of mesoscale self‐organization. The tiling pattern has been observed for the other materials on much shorter length‐scale; therefore, the experimental fact observed in the present study is demonstrating that the complexity is universal over different hierarchies. © 2005 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 43: 2427–2432, 2005
An open‐label study of the effects of bupropion SR on fatigue, depression and quality of life of mixed‐site cancer patients and their partners
Authors: Moss Erin L. , Simpson J. Steven A. , Pelletier Guy , Forsyth Peter 
Published: July 2005

AbstractThis preliminary study investigated whether bupropion sustained release (SR) improved symptomatic fatigue, depression and quality of life in cancer patients and caregiver quality of life. The sample consisted of a prospective open case series of 21 cancer patients, with fatigue and with or without depression at moderate to severe levels, referred for psychiatric assessment from a tertiary care cancer centre. Both patient symptom ratings and caregiver ratings were measured before and after 4 weeks of treatment with the maximally tolerated dose of bupropion in the range of 100–300 mg per day. At trial completion, significant improvement was found for symptoms of fatigue and depression. Subjects were divided into two groups: depressed and non‐depressed (based on a cut‐off score of 17 on the Hamilton Depression Rating Scale). Both groups reported improvement for fatigue and depressive symptoms. Depressed subjects and their caregivers did not experience any change in quality of life, while the non‐depressed subjects and their caregivers reported improvements. Results from this small group of patients suggest that bupropion may have potential as an effective pharmaceutical agent for treating cancer‐related fatigue. A randomized, placebo‐controlled trial with this medication is indicated. Copyright © 2005 John Wiley & Sons, Ltd.
Stability of decisional role preference over the course of cancer therapy
Authors: Mallinger Julie B. , Shields Cleveland G. , Griggs Jennifer J. , Roscoe Joseph A. , Morrow Gary R. , Rosenbluth Richard J. , Lord Raymond S. , Gross Howard 
Published: July 2005

AbstractCancer patients vary in their preferred level of involvement in medical decision making, and responding to patients' desired level of involvement is a key element of good medical care. While the literature has clearly demonstrated heterogeneity among cancer patients' preferences, less is known about how the preferences of any given patient may change over time. This longitudinal study compared cancer patients' preferences for involvement in medical decision making from the time of diagnosis to the time of completion of therapy. Data from 729 cancer patients with mixed diagnoses were analyzed. Most patients reported a change in preferred level of involvement over time, and multivariate analysis demonstrated that patients tend to prefer a decreasing level of involvement over time (p<0.0001). Stability of patients' preferences was also associated with type of cancer, but not with other sociodemographic characteristics. The results from this study highlight the importance of reevaluating patients' preferences for involvement in medical decision making throughout the course of cancer therapy, as such preferences are likely to change. Copyright © 2005 John Wiley & Sons, Ltd.
Cancer‐related health worries and psychological distress among older adult, long‐term cancer survivors
Authors: Deimling Gary T. , Bowman Karen F. , Sterns Samantha , Wagner Louis J. , Kahana Boaz 
Published: July 2005

AbstractWhile long‐term survivors (5 years+) do not face the stressors of diagnosis and treatment, they continue to face the uncertainties that survivorship brings: recurrence, other cancers, late effects of treatment, and the potential of a shortened life expectancy. This research focuses on the cancer‐related health worries of older adult, long‐term cancer survivors, the factors that predict these worries, and their link to traditional measures of psychological distress. Specifically, a model is proposed that identifies the personal (including race and gender) and illness/treatment characteristics of survivors that are significantly associated with cancer‐related health worries and their effects on anxiety and depression. Descriptive and multivariate analyses of a random sample of 321 long‐term survivors in a major cancer center tumor registry are used to address these issues. About one‐third of survivors continue to report worries about recurrence, worries about a second cancer, and worries that symptoms they experience may be from cancer. The regression analyses show that cancer‐related health worries is a significant predictor of both depression (beta=0.36) and anxiety (beta=0.21). Race is a significant predictor; being African American is related to fewer cancer‐related health worries (beta=−0.22). Having more symptoms during treatment is also a predictor of having more cancer‐related health worries (beta=0.20). The most consistent predictor of psychosocial distress is dispositional optimism/pessimism, with more optimistic individuals reporting fewer cancer‐related health worries (beta=−0.27), lower levels of both anxiety (beta=−0.16) and depression (beta=−0.23). Overall, for many older adult, long‐term survivors, the legacy of cancer continues in terms of cancer‐related health worries. In spite of these, for most survivors, their quality of life is not dramatically compromised either physically or psychologically. Copyright © 2005 John Wiley & Sons, Ltd.
Interactions of macromolecules with salt ions: An electrostatic theory for the Hofmeister effect
Authors: Zhou Huan‐Xiang 
Published: July 2005

AbstractSalting‐out of proteins was discovered in the nineteenth century and is widely used for protein separation and crystallization. It is generally believed that salting‐out occurs because at high concentrations salts and the protein compete for solvation water. Debye and Kirkwood suggested ideas for explaining salting‐out (Debeye and MacAulay, Physik Z; 1925;131:22–29; Kirkwood, In: Proteins, amino acids and peptides as ions and dipolar ions. New York: Reinhold; 1943. p 586–622). However, a quantitative theory has not been developed, and such a theory is presented here. It is built on Kirkwood's idea that a salt ion has a repulsive interaction with an image charge inside a low dielectric cavity. Explicit treatment is given for the effect of other salt ions on the interaction between a salt ion and its image charge. When combined with the Debye‐Hückel effect of salts on the solvation energy of protein charges (i.e., salting‐in), the characteristic curve of protein solubility versus salt concentration is obtained. The theory yields a direct link between the salting‐out effect and surface tension and is able to provide rationalizations for the effects of salt on the folding stability of several proteins. Proteins 2005. © 2005 Wiley‐Liss, Inc.
BAliBASE 3.0: Latest developments of the multiple sequence alignment benchmark
Authors: Thompson Julie D. , Koehl Patrice , Ripp Raymond , Poch Olivier 
Published: July 2005

AbstractMultiple sequence alignment is one of the cornerstones of modern molecular biology. It is used to identify conserved motifs, to determine protein domains, in 2D/3D structure prediction by homology and in evolutionary studies. Recently, high‐throughput technologies such as genome sequencing and structural proteomics have lead to an explosion in the amount of sequence and structure information available. In response, several new multiple alignment methods have been developed that improve both the efficiency and the quality of protein alignments. Consequently, the benchmarks used to evaluate and compare these methods must also evolve. We present here the latest release of the most widely used multiple alignment benchmark, BAliBASE, which provides high quality, manually refined, reference alignments based on 3D structural superpositions. Version 3.0 of BAliBASE includes new, more challenging test cases, representing the real problems encountered when aligning large sets of complex sequences. Using a novel, semiautomatic update protocol, the number of protein families in the benchmark has been increased and representative test cases are now available that cover most of the protein fold space. The total number of proteins in BAliBASE has also been significantly increased from 1444 to 6255 sequences. In addition, full‐length sequences are now provided for all test cases, which represent difficult cases for both global and local alignment programs. Finally, the BAliBASE Web site (http://www‐bio3d‐igbmc.u‐strasbg.fr/balibase) has been completely redesigned to provide a more user‐friendly, interactive interface for the visualization of the BAliBASE reference alignments and the associated annotations. Proteins 2005. © 2005 Wiley‐Liss, Inc.
Different methylation characteristics of protein arginine methyltransferase 1 and 3 toward the Ewing Sarcoma protein and a peptide
Authors: Pahlich Steffen , Bschir Karim , Chiavi Claudio , Belyanskaya Larisa , Gehring Heinz 
Published: July 2005

AbstractThe multifunctional Ewing Sarcoma (EWS) protein, a member of a large family of RNA‐binding proteins, is extensively asymmetrically dimethylated at arginine residues within RGG consensus sequences. Using recombinant proteins we examined whether type I protein arginine methyltransferase (PRMT)1 or 3 is responsible for asymmetric dimethylations of the EWS protein. After in vitro methylation of the EWS protein by GST‐PRMT1, we identified 27 dimethylated arginine residues out of 30 potential methylation sites by mass spectrometry‐based techniques (MALDI‐TOF MS and MS/MS). Thus, PRMT1 recognizes most if not all methylation sites of the EWS protein. With GST‐PRMT3, however, only nine dimethylated arginines, located mainly in the C‐terminal region of EWS protein, could be assigned, indicating that structural determinants prevent complete methylation. In contrary to previous reports this study also revealed that trypsin is able to cleave after methylated arginines. Pull‐down experiments showed that endogenous EWS protein binds efficiently to GST‐PRMT1 but less to GST‐PRMT3, which is in accordance to the in vitro methylation results. Furthermore, methylation of a peptide containing different methylation sites revealed differences in the site selectivity as well as in the kinetic properties of GST‐PRMT1 and GST‐PRMT3. Kinetic differences due to an inhibition effect of the methylation inhibitor S‐adenosyl‐L‐homocysteine could be excluded by determining the corresponding Ki values of the two enzymes and the Kd values for the methyl donor S‐adenosyl‐L‐methionine. The study demonstrates the strength of MS‐based methods for a qualitative and quantitative analysis of enzymic arginine methylation, a posttranslational modification that becomes more and more the object of investigations. Proteins 2005. © 2005 Wiley‐Liss, Inc.
Mass spectrometric investigation of gaseous YbH, YbO and YbOH molecules
Authors: Brutti S. , Terai T. , Yamawaki M. , Yasumoto M. , Balducci G. , Gigli G. , Ciccioli A. 
Published: July 2005

AbstractThe high‐temperature gaseous molecules YbH, YbO and YbOH have been identified and their thermochemistry investigated by the Knudsen effusion mass spectrometry technique coupled with a controlled pressure gas inlet system. Solid ytterbium monosilicide and disilicide samples were made to react in the Knudsen cell with H2(g) and H2(g)/O2(g); in these conditions, several gaseous species (Yb, YbO, YbH, YbOH, SiO, SiO2, H2O) were formed under equilibrium conditions. The temperature dependences of the partial pressures of the observed gaseous molecules were analyzed to derive the YbX bond energies (X = H, O, OH). Selected values are D(YbH) = 179.4 ± 2.0 kJ mol−1, D(YbO) = 384 ± 10 mol−1 and D(YbOH) = 322 ± 12 kJ mol−1, and ΔatH(YbOH) = 746 ± 12 kJ mol−1. Density functional theory (DFT) calculations were also performed. Experimental and computational results are discussed and compared to previous data when available. The SiO/SiO2 high‐temperature gaseous equilibrium was also observed. Copyright © 2005 John Wiley & Sons, Ltd.
Analysis of vitamin E derivatives in serum using coordinated ion spray mass spectrometry
Authors: Al‐Talla Zeyad A. , Tolley Luke T. 
Published: July 2005

AbstractA method for the extraction and analysis of tocopherols from serum using coordinated ion spray (CIS) mass spectrometry was developed and tested. The tocopherols were extracted from serum and analyzed by direct infusion into the mass spectrometer, bypassing the need for a liquid chromatography step. CIS is a method for improving the ionization efficiency of non‐polar compounds by adding metal ions to the electrospray solvent. The non‐polar analytes appear as metal adducts in the resulting mass spectrum. Silver was used as the metal ion for the CIS, causing analyte masses to be increased by 107 and 109 Da from the two main silver isotopes. Vitamin E succinate was added to the samples before extraction and was used as an internal standard to compensate for any variations in the extraction efficiency or mass spectrometric response. α‐Tocopherol and an ether‐linked analogue known as α‐TEA were analyzed in concentrations from 1.25–40 µg/mL (1.9–60 pg consumed). The response curve was constructed by comparing the response of the analytes to the internal standard and gave linear results with r2 values greater than 0.98. This new method was shown to be sensitive, reproducible, fast and required very small amounts of analyte. Copyright © 2005 John Wiley & Sons, Ltd.
Characteristic pH and electrolyte concentration dependences of 2‐aminopyridine‐derivatized oligosaccharides (N‐glycans) in sonic‐spray ionization mass spectrometry
Authors: Deguchi Kisaburo , Takegawa Yasuhiro , Hirabayashi Atsumu , Nakagawa Hiroaki , Nishimura Shin‐Ichiro 
Published: July 2005

AbstractThe intensities of ion signals from neutral oligosaccharides (N‐glycans) derivatized with 2‐aminopyridine (PA) were analyzed by ion trap mass spectrometry with a sonic‐spray ionization (SSI) source, in both positive‐ and negative‐ion modes, while varying the pH and concentration of ammonium acetate buffer solution. Two characteristic results are reported and discussed. The first characteristic is the pH dependence of the ion intensities; on increasing the solution pH from 4.3 to 8.6, positive ion intensities increase and negative ion intensities decrease. The second characteristic concerns the dependence of ion intensities on electrolyte concentration; on increasing the electrolyte concentration, the SSI efficiency for the PA N‐glycans first increases and then decreases. Assuming that the SSI mechanism essentially conforms to the statistical charging model and the charge residue model, a new model that focuses a great deal of attention on the counter (electrolyte) ion distribution surrounding the solvated analyte (PA N‐glycan) is proposed, in particular to rationalize the characteristic pH dependence. Copyright © 2005 John Wiley & Sons, Ltd.
Application of electrospray ionization with Fourier transform ion cyclotron resonance mass spectrometry for structural screening of core oligosaccharides from lipopolysaccharides of the bacteria Proteus
Authors: Kondakova Anna N. , Vinogradov Evgeny V. , Knirel Yuriy A. , Lindner Buko 
Published: July 2005

AbstractElectrospray ionization with Fourier transform ion cyclotron resonance mass spectrometry (ESI‐FTICRMS) was used for screening and structural elucidation of core oligosaccharides isolated from lipopolysaccharides of bacteria of the genus Proteus. Mass spectra allowed the determination of the molecular masses with high accuracy and the estimation of the chemical heterogeneity of the samples. They did not, however, provide sufficient information to identify structural details of the branched oligosaccharides. Therefore, various fragmentation techniques for determining such details were examined. Infrared multiphoton dissociation tandem mass spectrometry (IRMPD‐MS/MS) experiments in negative ion mode resulted in cleavage between the structurally conserved inner core region and the variable outer core region. Positive ion capillary skimmer dissociation mass spectra showed numerous fragment ion peaks, including those corresponding to the subsequent cleavage of the glycosidic linkages starting from the non‐reducing end of the oligosaccharide. Despite their complexity, these mass spectrometric studies allowed confirmation of previously determined Proteus lipopolysaccharide core structures, and identification of new related structures in other strains of these bacteria. Copyright © 2005 John Wiley & Sons, Ltd.
A rapid sample preparation method for mass spectrometric characterization of N‐linked glycans
Authors: Yu Ying Qing , Gilar Martin , Kaska Jennifer , Gebler John C. 
Published: July 2005

AbstractA rapid method for analysis of glycans of glycoproteins is presented. This method comprised deglycosylation, sample cleanup and matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) analysis of glycans. The enzymatic deglycosylation of N‐linked glycoproteins was enhanced in terms of speed and reproducibility using an enzyme‐friendly surfactant. The released glycans were desalted using a micro‐scale solid phase extraction (SPE) device packed with a hydrophilic interaction chromatography (HILIC) sorbent. Hydrophilic glycans were well retained by SPE, while salts and surfactants were removed from the sample. The glycans were eluted using 25–50 µL of solvent and analyzed directly without derivatization using MALDI‐MS. MALDI quadrupole time‐of‐flight (Q‐Tof) instrumentation was utilized for glycan profiling and structure characterization by tandem mass spectrometry (MS/MS). The presented method allows sensitive analysis of glycans benefiting from optimized deglycosylation reactions and efficient sample cleanup. Copyright © 2005 John Wiley & Sons, Ltd.