bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Profiling Method for Oxylipins in Neurodegenerative Diseases by Liquid Chromatography Coupled with Tandem Mass Spectrometry
Authors: Pourmand Elham , Zhang Fan , Sarparast Morteza , Alan Jamie K. , Lee Kin Sing Stephen 
Published: October 2023

AbstractAging is one of the major risk factors for many chronic diseases, including diabetes, neuropathy, hypertension, cancer, and neurodegenerative diseases. However, the mechanism behind aging and how aging affects a variety of disease progression remains unknown. Recent research demonstrated the cytochrome P450 (CYP)-epoxide hydrolase (EH) metabolites of polyunsaturated fatty acids (PUFAs) play a critical role in the abovementioned age-associated diseases. Therefore, aging could affect the abovementioned chronic diseases by modulating CYP-EH PUFA metabolism. Unfortunately, investigating how aging affects CYP-EH metabolism in human and mammalian models poses significant challenges.In this regard, we will useC. elegansas a model organism to investigate the aging effects on CYP-EH metabolism of PUFA, owing to its long history of being used to study aging and its associated benefits of conducting aging research. This project will develop analytical tools to measure the endogenous levels of CYP-EH PUFA metabolites inC. elegansusing state-of-the-art ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). These metabolites are very potent but present in low abundance. The dramatic increase in sensitivity in UPLC-MS/MS allows us to monitor these metabolites over the lifespan of C.eleganswith minimum samples. Our results show thatC. elegansproduces similar CYP PUFA metabolites to mammals and humans using our SPE-UPLC-MS/MS method. We will also show that our method successfully determined the CYP-EH PUFA metabolites profile changes induced by the inhibition ofC. elegansEH. The method developed from this project will significantly improve our understanding of the role of dietary PUFAs and associated metabolism on aging and neurodegeneration and will uncover new mechanisms of how aging affects neurodegeneration through the modulation of PUFA metabolic pathways.Graphical abstract
Decoding transcriptomic signatures of Cysteine String Protein alpha-mediated synapse maintenance
Authors: Wang Na , Zhu Biqing , Allnutt Mary Alice , Grijalva Rosalie M. , Zhao Hongyu , Chandra Sreeganga S. 
Published: October 2023

AbstractSynapse maintenance is essential for generating functional circuitry and decrement in this process is a hallmark of neurodegenerative disease. While we are beginning to understand the basis of synapse formation, much less is known about synapse maintenancein vivo. Cysteine string protein α (CSPα), encoded by theDnajc5gene, is a synaptic vesicle chaperone that is necessary for synapse maintenance and linked to neurodegeneration. To investigate the transcriptional changes associated with synapse maintenance, we performed single nucleus transcriptomics on the cortex of young CSPα knockout (KO) mice and littermate controls. Through differential expression and gene ontology analysis, we observed that both neurons and glial cells exhibit unique signatures in CSPα KO brain. Significantly all neurons in CSPα KO brains show strong signatures of repression in synaptic pathways, while upregulating autophagy related genes. Through visualization of synapses and autophagosomes by electron microscopy, we confirmed these alterations especially in inhibitory synapses. By imputing cell-cell interactions, we found that neuron-glia interactions were specifically increased in CSPα KO mice. This was mediated by synaptogenic adhesion molecules, including the classical Neurexin1-Neuroligin 1 pair, suggesting that communication of glial cells with neurons is strengthened in CSPα KO mice in an attempt to achieve synapse maintenance. Together, this study reveals unique cellular and molecular transcriptional changes in CSPα KO cortex and provides new insights into synapse maintenance and neurodegeneration.Significance statementSynapse maintenance is important for maintaining neuronal circuitry throughout life. However, little is known about molecules that affect synapse maintenancein vivo. CSPα, encoded by theDnajc5gene, is a synaptic vesicle chaperone that is linked to synapse maintenance and neurodegeneration. Here, we show by performing single nucleus transcriptomics of CSPα KO cortex that synapse instability is related to repression in synaptic pathways and elevation of autophagy in neurons. However, we find a heterogeneity of glial responses. Additionally, interactions between neurons and glia are increased in CSPα KO, mediated by synaptogenic adhesion molecules. This study provides a novel perspective on into synapse maintenance and reveals unique cellular and molecular transcriptional changes in CSPα KO brains.
Spatially and Functionally Distinct mTORC1 Entities Orchestrate the Cellular Response to Amino Acid Availability
Authors: Fernandes Stephanie A. , Angelidaki Danai-Dimitra , Nüchel Julian , Pan Jiyoung , Gollwitzer Peter , Elkis Yoav , Artoni Filippo , Wilhelm Sabine , Kovacevic-Sarmiento Marija , Demetriades Constantinos 
Published: October 2023

AbstractAmino acid (AA) availability is a robust determinant of cell growth, through controlling mTORC1 activity1. According to the predominant model in the field, AA sufficiency drives the recruitment and activation of mTORC1 on the lysosomal surface by the heterodimeric Rag GTPases, from where it coordinates the majority of cellular processes (reviewed in2,3). Importantly, however, 15 years after its initial discovery, the teleonomy of the proposed lysosomal regulation of mTORC1, and where mTORC1 acts on its effector proteins remain enigmatic4. Here, by using multiple pharmacological and genetic means to perturb the lysosomal AA sensing and protein recycling machineries, we describe the spatial separation of mTORC1 regulation and downstream functions in mammalian cells, with lysosomal and non-lysosomal mTORC1 phosphorylating distinct substrates in response to different AA sources. Moreover, we reveal that a fraction of mTOR localizes at lysosomes due to basal lysosomal proteolysis that locally supplies new AAs, even in cells grown in the presence of extracellular nutrients, whereas cytoplasmic mTORC1 is regulated by exogenous AAs. Overall, our study substantially expands our knowledge about the topology of mTORC1 regulation by AAs, and hints at the existence of distinct, Rag- and lysosome-independent mechanisms that control its activity at other subcellular locations. Given the importance of mTORC1 signalling and AA sensing for human ageing and disease2, our findings will likely open new directions toward the identification of function-specific mTORC1 regulators, and suggest new targets for drug discovery against conditions with dysregulated mTORC1 activity in the future.
An enzyme-free, cold-process acoustic method for gentle and effective tissue dissociation
Authors: MacMullan Melanie A. , Busalacchi Marlee , Quisling Sophie , Quast Brian , Pullen Johnna , Addington Sydney , Vivek Vibhu , Levers Steve , Renkema Kristin 
Published: October 2023

AbstractAs biological advances continue to improve the resolution of genomic and proteomic studies, the quality of single cell suspensions is becoming increasingly important. While conventional approaches use enzymes which may require heat Abbreviations: Bulk Lateral Ultrasound (BLU) activation to break down extracellular tissue matrices and gain access to single cells, recent studies have suggested that these harsh biochemical and heat-based treatments may result in genomic and proteomic modulation. To minimize these dissociation artifacts, we have developed an instrument for dissociating cells from various tissue matrices using Bulk Lateral Ultrasound (BLU™) energy. This enzyme-free, gentle mechanical dissociation maintains sample temperatures below 8°C for the duration of processing, resulting in high-fidelity single cell suspensions with comparable viability and live cell counts to those obtained with conventional enzymatic dissociations. Here, in murine-derived brain, heart, lung, and B16 melanoma tumor tissue dissociated by either BLU or by a commercially available dissociation kit which uses enzymes and heat, we compare cell viability and expression of population-specific immunological markers. The dramatic differences observed in cell surface expression suggest that cells dissociated using enzymes and heat may be experiencing stress-induced changes post-harvest that could impact conclusions and impede research progress. Alternatively, using gentle mechanical dissociation with BLU, we demonstrate the preservation of these markers and enable a minimally invasive alternative to obtaining high integrity single cell suspensions.HighlightsNovel, acoustic energy-based, enzyme-free dissociation improves single cell suspensionsEnzymatic dissociation diminishes cell counts, viability, and surface marker expressionImmunophenotyping reveals marker preservation by acoustic-based dissociaton
Does economic policy uncertainty matter for a firm's leverage and speed of adjustment?
Authors: Aftab Muhammad , Shehzadi Saman , Qureshi Fiza 
Published: September 2023

PurposeThis research intends to investigate the impact of economic policy uncertainty (EPU) on the firm's leverage and its adjustment speed.Design/methodology/approachThis study applies dynamic panel data modeling by using a partial adjustment model. The study is based on secondary data of the non-financial firms that are listed on the Pakistan stock exchange. For the analysis purpose, the study applies the generalized method of moments (GMM) estimation technique and uses a newly developed text-based measure of economic policy uncertainty.FindingsThe results show the negative impact of EPU on leverage decisions but a positive impact of EPU on leverage speed of adjustment for both, short-run and long-run economic policy shocks. Additional analysis reveals that the negative influence of long-run policy shocks on leverage decisions is moderated through profitability, and the negative influence of short-run policy shocks on leverage is moderated through firm size, tangibility and available growth prospects. However, the significant positive impact of EPU on the leverage speed of adjustment in both short and long-term policy shocks indicates that the speed of adjustment for these firms is not affected by policy shocks.Originality/valueThis research contributes to the existing literature on capital structure dynamics,by investigating the impact of EPU on firm financing decisions and estimating the adjustment speed of capital structure in a developing market context. The study also extends the existing literature by applying the concept of long-run and short-run economic policy uncertainty in the capital structure dynamic framework. Additionally, the new news-based measure of EPU is used. Moreover, it also looks into the COVID-19 effect on the relationship.
How healthy are employee-related CSR initiatives: a mapping and discussion of applying health-related employee benefits as part of companies' CSR programs
Authors: Schmeltz Line , Nisbeth Brøgger Matilde 
Published: September 2023

PurposeThe aim of this study is to investigate the prevalence of corporate health initiatives as part of CSR, and how and to what extent these initiatives are communicated in CSR reports.Design/methodology/approachThe study comprises two strands. First, a mapping of the extent and nature of health-related CSR initiatives among 11 selected Danish companies is developed, based on a qualitative email questionnaire. Next, the mapping serves as the framework for coding and analyzing CSR reports from 2018 to 2020 from 15 companies (45 in total).FindingsThe study provides a typology of the extent and nature of such CSR initiatives consisting of more than 50 types of health-related initiatives. Analysis of the CSR reports illustrates an increase in the explicit communication of employee health initiatives as well as the number of different categories applied over the three years.Practical implicationsThe study provides CSR managers with awareness about the extent and usage of employee health as part of corporate communication on CSR, including how such initiatives can be communicated in the CSR report. It also gives rise to carefully considering the potential negative implications for employees when planning health initiatives.Originality/valueThe study is the first of its kind to combine the perspectives of CSR and workplace health promotion to discuss the potential institutionalization of employee health as part of the corporate CSR program.
Multilatinas’ foreign divestment and host country institutional uncertainty: is there a best entry strategy?
Authors: da Fonseca Luíza Neves Marques , da Rocha Angela , Ferreira Jorge Brantes 
Published: October 2023

Purpose
This paper aims to investigate the divestment behavior of emerging market multinationals from Latin America – multilatinas – by examining how their foreign market entry decision impacts the likelihood of subsidiary divestment.

Design/methodology/approach
The hypotheses are tested using Cox’s proportional hazard rate model in a longitudinal database of Brazilian multinational companies established in 43 countries.

Findings
Results indicate that these subsidiaries can thrive in environments that bear similarities to their home country, being less likely to divest in institutionally weak countries. Contrary to developed country multinationals, these firms benefit from foreign entry decisions that entail handling partnerships abroad; thus, wholly-owned greenfield (WOGF) investments have a higher likelihood of being divested.

Originality/value
To the best of the authors’ knowledge, this paper is the first to analyze foreign divestment from multilatinas, accounting for how entry mode strategy and host country institutions may impact these firms’ de-internationalization.
Kantian–Nashian interaction and petty tax corruption in developing countries
Authors: Tran-Nam Binh 
Published: September 2023

PurposeThis paper attempts to develop a simple, static model of tax administration that is capable of explaining the widespread collusive petty tax administration corruption observed in developing countries.Design/methodology/approachThis paper utilizes a positivist research framework and adopts a theoretical method of analysis, although secondary data will also be mentioned to support theoretical arguments whenever it is appropriate to do so.FindingsA high rate of collusive tax corruption is inevitable in developing countries.Research limitations/implicationsThe model is static and needs to be extended into a dynamic model.Practical implicationsTraditional enforcement tools such as higher audits or a higher penalty regime against tax evasion do not work. Tax simplification can lessen the incidence of tax corruption.Social implicationsFighting tax corruption requires significant changes in the attitudes of taxpayers and tax auditors.Originality/valueThis paper combines the literature on Kantian economics and tax compliance in an innovative fashion.
The behavior of Islamic and conventional banks around the pandemic: cross-country evidence
Authors: Ghosh Saibal 
Published: September 2023

PurposeUsing cross-country data on the 1,000 largest global banks for 2019, the paper aims to examine the response of bank risk and returns to the pandemic.Design/methodology/approachThe author employs weighted least squares (WLS) techniques for the purposes of analysis.FindingsThe findings suggest that banks with Islamic windows increased their riskiness in response to the pandemic, although there was not much impact on profitability. Additionally, the author categorizes banks based on certain major characteristics and find that these findings are manifest primarily for well-capitalized and less liquid banks.Originality/valueResearch as to the impact of the pandemic on banks' balance sheets has been an unaddressed area of research. By focusing on a large sample of banks across countries with both Islamic and conventional banking presence, the analysis sheds light on the balance sheet response of banks to the pandemic, an aspect that has not been addressed earlier.
The effect of teacher engagement factors on teachers' lesson study professional development in Chinese school settings
Authors: Ji Ying 
Published: September 2023

PurposeThis study aimed to examine the influence of four factors (intellectual, instrumental, self-actualizing and CPD cultural factors) on teachers' engagement in continuing professional development (CPD), specifically in the context of three lesson study activities: teaching research team activity, teaching research group activity and research lesson study activity in Chinese school settings.Design/methodology/approachA mixed-methods design was employed to examine the perceived effects of these factors on the engagement levels of 33 primary school teachers who participated in lesson study activities. The research used a questionnaire survey and semi-structured interviews as data collection instruments.FindingsThe results indicated that participants demonstrated higher levels of engagement in research lesson study and teaching research group, whereas their engagement in teaching research team was comparatively lower. Intellectual and CPD cultural factors emerged as the strongest predictors of engagement in all the activities. Self-actualizing factors highly influenced teachers' engagement in research lesson study. Instrumental factors mainly affected novice teachers and some mentors in the teaching research group, but not others.Originality/valueThis study addresses a gap in the existing literature by investigating factors influencing teachers' engagement in lesson study professional development. The observed variation in teachers' engagement behaviors and attitudes provides policy makers and school leaders with a deeper understanding of the underlying mechanisms and effects of these engagement factors on teachers' professional development.
Transfer pricing practices in multinational corporations and their effects on developing countries' tax revenue: a systematic literature review
Authors: Kalra Akash , Afzal Munshi Naser Ibne 
Published: September 2023

PurposeFor many global firms and corporate oligopolies, transfer pricing is essential. The transfer pricing literature as it is currently written is succinctly summarized in this study. The authors offer a thorough analysis of transfer pricing research in this study. This review sheds light on the top researchers, approaches, conclusions, theoretical and empirical gaps, and upcoming issues of transfer pricing research over the previous nine years through a methodical analysis of 29 research publications from the Scopus database (2014–2022). To help graduate students pursue further degrees in this area, such as a master's, thesis or PhD, this study will highlight five research issues.Design/methodology/approachThis essay looks at five significant areas of tax avoidance and transfer pricing research. Some of these issues include determining the impact of transfer pricing regulations on various types of multinational corporations, assessing the effectiveness of transfer pricing regulations in preventing tax evasion, examining various policy options and determining the impact of transfer pricing on other economic outcomes using a systematic literature review.FindingsThe findings of this review demonstrate the need for transfer pricing research to look more closely at transfer pricing as a tool for business in addition to compliance and tax management.Originality/valueThis analysis concludes with future directions for transfer pricing research.
On the traces of documentation: German approach and its pioneers
Authors: Aparac-Jelušić Tatjana 
Published: September 2023

PurposeThe main purpose of the paper is to offer a personal view on the development of documentation/information and documentation (IuD) in Germany, while pointing out the need to further investigate the specific features of its development paths. The methodology is based on critical review of the available literature sources in the German language.Design/methodology/approachThe paper uses the method of critical review of published documents in journals (especially in Nachrichten für Dokumentation), books and reports of state and provincial administrations that are directly related to monitoring and/or encouraging the development of the young field of documentation.FindingsThe paper offers a review and interpretation of the most significant development phases, the contributions of individuals and the influence of the official state and information policy based on the consulted sources.Research limitations/implicationsThis research is limited to the literature written in German language.Practical implicationsThe paper could be of interest to researchers and professionals who are interested in the development of documentation.Social implicationsThe paper covers the period after the World War II until the end of 1980s that is especially interesting from the social point of view in divided Germany.Originality/valueTo the author’s knowledge, there is no comprehensive history of documentation in German-speaking countries written in English. This paper is the result of a research project started three years ago with colleagues from Germany, Austria and Switzerland, that aims to cover all phases of the appearance and development of information science in German-speaking countries and could be understood as a kind of introduction to papers planned to follow.
Green brands, customer satisfaction and sustainable performance in the Chinese manufacturing industry
Authors: Waqas Muhammad , Qingfeng Meng , Ahmad Naveed , Iqbal Muzaffar 
Published: October 2023

PurposeEnvironmental sanctions and pressure from governments and customers can play an essential role in driving the manufacturing industry to adopt more sustainable practices and reduce their environmental impact. Thus, this study explores how pressures from customers and governments influence sustainable firm performance and customer satisfaction through green production, green creativity, green brand innovativeness and green brand image in the Chinese manufacturing industry? Further, the moderating role of green knowledge was analyzed.Design/methodology/approach640 valid responses from the Chinese manufacturing industry were collected through web-based and physical surveys and later assessed through structural equation modeling.FindingsThe findings of this study validated the direct relationship between the proposed construct. Mediation analysis found the mediating role of green brand innovativeness, green brand image green and customer satisfaction. Furthermore, the moderating impact of green knowledge was also proved.Originality/valueThe empirical findings of this research filled the literature gap on cleaner production and sustainable firm performance by focusing on green production, green creativity and green knowledge in the Chinese manufacturing industry context.