bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fast proliferating and slowly migrating non‐small cell lung cancer cells are vulnerable to decitabine and retinoic acid combinatorial treatment
Authors: Pelos Giulia , Riester Marisa , Pal Jagriti , Myacheva Ksenia , Moneke Isabelle , Rotondo John Charles , Lübbert Michael , Diederichs Sven 
Published: November 2023

AbstractNon‐small cell lung cancer (NSCLC) patients are often elderly or unfit and thus cannot tolerate standard aggressive therapy regimes. In our study, we test the efficacy of the DNA‐hypomethylating agent decitabine (DAC) in combination with all‐trans retinoic acid (ATRA), which has been shown to possess little systemic adverse effects. Screening a broad panel of 56 NSCLC cell lines uncovered a decrease in cell viability after the combination treatment in 77% of the cell lines. Transcriptomics, proteomics, proliferation and migration profiling revealed that fast proliferating and slowly migrating cell lines were more sensitive to the drug combination. The comparison of mutational profiles found oncogenic KRAS mutations only in sensitive cells. Additionally, different cell lines showed a heterogeneous gene expression response to the treatment pointing to diverse mechanisms of action. Silencing KRAS, RIG‐I or RARB partially reversed the sensitivity of KRAS‐mutant NCI‐H460 cells. To study resistance, we generated two NCI‐H460 cell populations resistant to ATRA and DAC, which migrated faster and proliferated slower than the parental sensitive cells and showed signs of senescence. In summary, this comprehensive dataset uncovers a broad sensitivity of NSCLC cells to the combinatorial treatment with DAC and ATRA and indicates that migration and proliferation capacities correlate with and could thus serve as determinants for drug sensitivity in NSCLC.
Risk of thrombocytopenia in neonates of thrombocytopenic mothers
Authors: Houri Ohad , Sigal Sharon , Houri Oz , Brzezinski‐Sinai Noa A. , Gomez Tolub Rachel , Berezowsky Alexandra , Danieli Gruber Shir , Hadar Eran 
Published: November 2023

AbstractObjectiveMaternal thrombocytopenia during pregnancy may occur due to several possible etiologies, with potential neonatal impact. The aim of the present study was to investigate whether there is a correlation between maternal and neonatal platelet count among women with thrombocytopenia during pregnancy.MethodsA cross‐sectional retrospective study (2012–2019) was conducted at a tertiary medical center. Complete blood count was routinely measured in all patients on admission to the delivery ward. Thrombocytopenia was defined as a platelet count below 150 K/μL. Clinical and outcome parameters of thrombocytopenic mothers and their newborns were collected from the electronic files and analyzed by severity of maternal thrombocytopenia.ResultsOf 45 385 women with a documented platelet count at admission, 2841 (6.24%) had thrombocytopenia: 2623 (5.7%) mild (100–149 K/μL), 207 (0.45%) moderate (50–99 K/μL), and 11 (0.02%) severe (<50 K/μL). Eight newborns had thrombocytopenia; corresponding rates by severity of maternal thrombocytopenia were 0.11%, 1.43%, and 18.18% (P = 0.04). None of the thrombocytopenic neonates had an intraventricular hemorrhage or other bleeding complications. The correlation between maternal and neonatal platelet counts was weak (Pearson r = 0.038; P = 0.046).ConclusionWe suggest that although the chances of neonatal thrombocytopenia are higher with worsening maternal thrombocytopenia, actual occurrence is rare, and the correlation is poor. Therefore, maternal thrombocytopenia cannot be regarded as a significant risk factor for neonatal thrombocytopenia. Neonatal platelet count should be obtained when maternal thrombocytopenia is autoimmune or less than 100 K/μL.
Operation and maintenance strategy of radio transmission equipment based on big data
Authors: Zhao Zhigang , Wang Lanzhong 
Published: November 2023

AbstractIn recent years, the operation and maintenance of radio transmission equipment have faced challenges such as equipment aging, high maintenance costs, long response time, and technological updates. It is necessary to adapt to new technologies and improve maintenance efficiency. The operation and maintenance strategy of radio transmitting equipment based on big data (BD) aims to optimize the reliability and operating efficiency of equipment through data analysis and intelligent algorithms, and reduce failure risk and maintenance costs. By analyzing the operation data and maintenance records of a large number of radio transmission equipment, it revealed the patterns and trends of equipment failures, in order to timely identify and predict possible faults, and take corresponding maintenance measures. This article adopted methods such as data collection and preprocessing, fault prediction and diagnosis, and maintenance optimization to achieve accurate monitoring of equipment operation status, fault prediction and diagnosis, optimized maintenance, and decision support. This can improve the operational efficiency and reliability of radio transmission equipment, which is used for fault diagnosis of radio transmission equipment through SVM (Support Vector Machine) algorithms. By training SVM classifiers, this article associated different types of faults with their feature data, thereby quickly and accurately determining the type of equipment fault based on real‐time collected device data, providing a basis for maintenance decision‐making. The experimental results show that the failure frequency of the radio transmitting equipment in the control group was between 7% and 11%, while the failure frequency of the radio transmitting equipment in the experimental group was between 3% and 7%. Experiments show that BD technology can effectively reduce the failure frequency of radio transmitting equipment. The empirical results indicate that this strategy can improve the reliability of equipment and reduce operation and maintenance costs, which has important guiding significance for the operation and maintenance management of radio equipment. This would bring about more efficient and reliable operation and maintenance management of radio equipment, making important contributions to the smooth progress of broadcasting business and the improvement of user experience.
Consumer acceptance of genetically engineered potatoes: The role of information and agreement
Authors: Lacy Katherine , McFadden Jonathan 
Published: November 2023

AbstractMany foods cooked at high temperatures produce acrylamide, a probable carcinogen, and neurotoxin. We evaluate how consumers' purchase intentions for potato products and subjective knowledge about conventional foods, genetically engineered (G.E.) foods, and acrylamide respond to information treatments. Information and prior purchases positively influence intentions to purchase potatoes but negatively influence purchase intentions for potato chips and French fries. Value‐of‐information calculations suggest industry‐focused and private‐company perspectives are worth $0.01‐$0.02/person per month. Our results have practical implications for food system actors seeking to better understand the broader determinants of consumer acceptance behaviors as they relate to G.E. foods that reduce health concerns.
Sprayable self‐assembly multifunctional bioactive poly(ferulic acid) hydrogel for rapid MRSA infected wound repair
Authors: Li Sihua , Ma Junping , Li Juntang , Qu Xiaoyan , Lei Bo 
Published: November 2023

AbstractThe repair of methicillin‐resistant staphylococcus aureus (MRSA) infected wounds remains a serious challenge. Development of multifunctional bioactive hydrogels has shown promising potential in treating MRSA wound. Ferulic acid has special bioactivities including antioxidant antiinflammation antibacterial capacities but limited in lack of engineering strategy for efficient treatment of MRSA infected wound. Herein, we developed a multifunctional bioactive poly(ferulic acid) copolymer (FPFA) for treating MRSA infected wound. FPFA could be self‐assembled into hydrogel under body temperature and demonstrated the injectable, sprayable, self‐healing, anti‐inflammatory, antioxidant, and angiogenic activity. FPFA hydrogel also showed the good cytocompatibility, efficiently enhanced the endothelial cell migration, scavenged intracellular reactive oxygen species (ROS), inhibited the expression of inflammatory factors and enhanced the in vitro angiogenesis. The MRSA‐infected wound model showed that FPFA could significantly inhibit the MRSA infection and excess inflammation, reinforce the angiogenesis, accelerate wound healing and skin tissue regeneration.
Patient‐Reported Decisional Regret After Operative Otolaryngology Procedures: A Scoping Review
Authors: Liu Alice Q. , McNeely Brendan D. , Prisman Eitan , Hu Amanda 
Published: November 2023

ObjectiveTo review the published literature on decisional regret in adult patients undergoing operative otolaryngology procedures. The primary outcome was decisional regret scale (DRS) scores. DRS scores of 0 indicate no regret, 1–25 mild regret, and >25 moderate to strong/severe regret.Data SourcesA comprehensive librarian‐designed strategy was used to search MEDLINE, Embase, and CINAHL from inception to September 2023.Review MethodsInclusion criteria consisted of English‐language studies of adult patients who underwent operative otolaryngology treatments and reported DRS scores. Data was extracted by two independent reviewers. Preferred Reporting Items for Systematic Reviews and Meta‐analyses Extension for Scoping Reviews (PRISMA‐ScR) guidelines were followed. Oxford Centre's Levels of Evidence were used for quality assessment.ResultsIn total, 6306 studies were screened by two independent reviewers; 13 studies were included after full‐text analysis. Subspecialties comprised: Head and neck (10), endocrine (1), general (1), and rhinology (1). The DRS results of the included studies spanned a mean range of 10.1–23.9 or a median range of 0–20.0. There was a trend toward more decisional regret after large head and neck procedures or when patients underwent multiple treatment modalities. Depression, anxiety, and patient‐reported quality of life measures were all correlated with decisional regret. Oxford Centre's Levels of Evidence ranged from 2 to 4.ConclusionThis is the first comprehensive review of decisional regret in otolaryngology. The majority of patients had no or mild (DRS <25) decisional regret after otolaryngology treatments. Future research on pre‐operative counseling and shared decision‐making to further minimize patient decisional regret is warranted.Level of EvidenceN/A Laryngoscope, 134:2562–2567, 2024
A novel method to sample individual marine snow particles for downstream molecular analyses
Authors: Baumas Chloé M.J. , Ababou Fatima‐Ezzahra , Garel Marc , Bizic Mina , Ionescu Danny , Puzenat Arthur , Le Moigne Frederic A.C. , Grossart Hans‐Peter , Tamburini Christian 
Published: November 2023

AbstractThe ocean–atmosphere exchange of carbon largely depends on the balance between carbon export of particulate organic carbon (POC) as sinking marine particles, and POC remineralization by attached microbial communities. Despite the vast spectrum of types, sources, ages, shapes, and composition of individual sinking particles, they are usually considered as a bulk together with their associated microbial communities. This limits our mechanistic understanding of the biological carbon pump (BCP) and its feedback on the global carbon cycle. We established a method to sample individual particles while preserving their shape, structure, and nucleic acids by placing a jellified RNA‐fixative at the bottom of drifting sediment traps. Coupling imaging of individual particles with associated 16S rRNA analysis reveals that active bacterial communities are highly heterogenous from one particles origin to another. In contrast to lab‐made particles, we found that complex in situ conditions lead to heterogeneity even within the same particle type. Our new method allows to associate patterns of active prokaryotic and functional diversity to particle features, enabling the detection of potential remineralization niches. This new approach will therefore improve our understanding of the BCP and numerical representation in the context of a rapidly changing ocean.
Influence of nitrogen content on the pit formation and pit propagation in the welded joints of X5CrNi18‐10 stainless steel
Authors: Radojković Bojana , Jegdić Bore , Pejić Jovanka , Marunkić Dunja , Simović Anđela , Eraković Pantović Sanja 
Published: November 2023

AbstractThe influence of welding current and nitrogen content in argon shielding gas on the resistance of the welded joint (weld metal and heat‐affected zone [HAZ]) of the stainless steel X5CrNi18‐10 to the formation and growth of pits was investigated. Also, the susceptibility of the welded joint to intergranular corrosion was examined. Pitting corrosion resistance indicators were determined based on anodic potentiodynamic polarization measurements in NaCl + Na2SO4 solution, while susceptibility to intergranular corrosion was determined by the potentiokinetic method with a double loop (DL EPR). SEM/EDS was used to analyze the microstructure. It has been shown that higher nitrogen content in shielding gas leads to an increase in the resistance of welded joints (weld metal and HAZ) to the pit formation. However, an improvement in the resistance to the pit formation leads to a decline in the resistance of the welded joint to pit growth. An explanation of this phenomenon is proposed. Also, it was shown that the increase of the welding current increases the susceptibility of the welded joint to intergranular corrosion, while the higher nitrogen content has no effect.
An inflammatory gene‐related prognostic risk score model for prognosis and immune infiltration in glioblastoma
Authors: Cheng Meixiong , Liu Ling , Zeng Yi , Li Zhili , Zhang Tian , Xu Ruxiang , Wang Qi , Wu Yaqiu 
Published: November 2023

AbstractThis study aimed to screen for key genes related to the prognosis of patients with glioblastoma (GBM). First, bioinformatics analysis was performed based on databases such as TCGA and MSigDB. Inflammatory‐related genes were obtained from the MSigDB database. The TCGA‐tumor samples were divided into cluster A and B groups based on consensus clustering. Multivariate Cox regression was applied to construct the risk score model of inflammatory‐related genes based on the TCGA database. Second, to understand the effects of model characteristic genes on GBM cells, U‐87 MG cells were used for knockdown experiments, which are important means for studying gene function. PLAUR is an unfavorable prognostic biomarker for patients with glioma. Therefore, the model characteristic gene PLAUR was selected for knockdown experiments. The prognosis of cluster A was significantly better than that of cluster B. The verification results also demonstrate that the risk score could predict overall survival. Although the immune cells in cluster B and high‐risk groups increased, no matching survival advantage was observed. It may be that stromal activation inhibits the antitumor effect of immune cells. PLAUR knockdown inhibits tumor cell proliferation, migration, and invasion, and promoted tumor cell apoptosis. In conclusion, a prognostic prediction model for GBM composed of inflammatory‐related genes was successfully constructed. Increased immune cell expression may be linked to a poor prognosis for GBM, as stromal activation decreased the antitumor activity of immune cells in cluster B and high‐risk groups. PLAUR may play an important role in tumor cell proliferation, migration, invasion, and apoptosis.
A case of congenital cataracts with hypotrichosis caused by compound heterozygous variants in the LSS gene
Authors: Tan Yu , Tian Huan , Mai Jingqun , Wang He , Yang Mei , Liu Shanling 
Published: November 2023

AbstractBackgroundPatients with biallelic variants in the lanosterol synthase (LSS) gene has been reported to exhibit phenotypes as follows: non‐syndromic form of hypotrichosis, congenital cataracts, and alopecia with intellectual disability or growth retardation. However, genotype–phenotype correlations in the LSS gene are still not completely clear.MethodsIn this study, we reported a Chinese girl who had congenital cataracts with hypotrichosis. The trio exome sequencing was performed to elucidate the genetic cause of the patient.ResultsWe identified compound heterozygous variants (c.296G>A, p.G99D and c.1025T>G, p.I342S) in the LSS gene. Both variants altered the amino acid coding at highly conserved amino acid residues and were predicted to be deleterious using prediction software.ConclusionOur report expands the spectrum of variants in the LSS gene and will be helpful for genotype–phenotype correlations study.
The importance of providing dietary fiber in medical and surgical critical care
Authors: McClave Stephen A. , Omer Endashaw , Eisa Mohamed , Klosterbauer Abby , Lowen Cynthia C. , Martindale Robert G. 
Published: November 2023

AbstractThe early provision of soluble/insoluble fiber to the patient who is critically ill has been controversial in the past. Especially in the setting of hemodynamic instability, dysmotility, or impaired gastrointestinal transit, fear of inspissation of formula with precipitation of nonocclusive mesenteric ischemia (NOMI)/nonocclusive bowel necrosis (NOBN) limited its utilization by medical and surgical intensivists. The incidence of NOMI/NOBN has been estimated at 0.2%–0.3% for all intensive care unit (ICU) patients receiving enteral nutrition (EN), and the occurrence of inspissated formula is even less. The science supporting a benefit from providing fiber has recently increased exponentially. The fermentation of soluble fibers leading to the production of short chain fatty acids supports gut barrier function, modulates immune responses, and promotes refaunation of commensal organisms. The “butyrate effect” refers to local (gastrointestinal tract) and systemic anti‐inflammatory responses mediated by the M2 polarization of macrophages, inhibition of histone deacetylase, and stimulation of ubiquitous G protein receptors. Both soluble and insoluble fiber have been shown to promote intestinal motility, reduce feeding intolerance, and shorten hospital length of stay. The benefit of providing dietary fiber early upon admission to the ICU outweighs its minimal associated risk. The point at which the intensivist determines that is safe to initiate EN, both soluble and insoluble fiber should be included in the enteral formulation.
Trends in the multiple prescriptions of hypnotic drugs in a university outpatient in Japan
Authors: Kato Takao , Kotorii Nozomu , Ozone Motohiro , Murotani Kenta , Ohshima Hayato , Mori Hiroyuki , Wasano Kenjirou , Hiejima Hiroshi , Habukawa Mitsunari , Uchimura Naohisa 
Published: November 2023

AbstractAimsIn Japan, the daily dosage of hypnotic drugs for insomnia treatment is increasing year by year, and over‐dependence on treatment with hypnotic drugs is a major problem. This study aimed to examine the factors related to the elimination of prescriptions of three or more hypnotic drugs within 1 year in our clinic.MethodsWe conducted two surveys. Survey ① assessed the frequency of prescriptions of three or more hypnotic drugs by retrospectively reviewing the medical records of all patients who visited general and psychiatric outpatient clinics from January 2013 to March 2019. Survey ② assessed changes in prescriptions of hypnotic and psychotropic drugs within the subsequent year by retrospectively reviewing the medical records of all patients prescribed three or more hypnotic drugs who visited neuropsychiatric outpatient clinics multiple times between April 2013 and March 2019.ResultsThe frequency of prescribing three or more hypnotic drugs was six to nine times higher in psychiatry than in other departments. Flunitrazepam and brotizolam were the most common drugs prescribed and had the second lowest discontinuation rate after zolpidem. Conversely, eszopiclone, zopiclone, and suvorexant had the highest discontinuation rates. The success factors for drug reduction were age (odds ratio [OR]: 0.97, p < 0.0037), trazodone addition (OR: 12.86, p < 0.0194) and number of years of psychiatric experience.ConclusionsThe characteristics and success factors in relation to drug reduction in patients with multiple prescriptions of hypnotic drugs identified in this study may contribute to solving the problem of multiple prescriptions of hypnotic drugs.
Thermostable ZnO/Ag@SiO2 nanohybrid material for extraordinary antibacterial activity polyester fibers
Authors: Zheng Shenyi , Chen Wei , Shi Chen , Han Jian 
Published: November 2023

AbstractMelt blending is considered a significant method to develop antibacterial textile materials. However, it is still challenging to the homogeneous dispersion and efficient antibacterial activity of inorganic nanoparticles in polymer fibers. In this work, ZnO/Ag@SiO2 inorganic nanohybrid antibacterial agents were prepared by the direct precipitation method. The prepared antibacterial material had efficient antibacterial and thermal stability properties. Specifically, the decomposition temperature of the ZnO/Ag@SiO2 was higher than 400°C, which was appropriate for melting polymers at high temperatures. Here, polyethylene terephthalate (PET) was used as a fiber matrix, and PET/ZnO/Ag@SiO2 fibers were prepared by melt spinning technology. The results showed that the antibacterial material was well dispersed in the PET matrix, and the crystallization was promoted during the cooling process. The modified PET fibers presented better antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with a maximum antibacterial rate of 99.9%. Additionally, the modified PET fibers provide superior laundering durability, with an antibacterial rate of 95.7% for S. aureus and 94.8% for E. coli after 50 washes. This work offers a novel approach to preparing thermotolerant and efficient antibacterial agents and antibacterial‐modified PET fibers.Highlights
ZnO/Ag@SiO2 nanohybrid antibacterial agents were prepared.
High temperature resistant and highly antibacterial material.
The particles dispersed uniformly in PET matrix.
Excellent antibacterial and laundering properties of the fiber
Synthesis of an alternating copolymer of the dense 1,2,3‐triazole backbone carrying t‐butyl ester and nitrile side chains
Authors: Xu Linlin , Nakahata Masaki , Kamon Yuri , Hashidzume Akihito 
Published: November 2023

AbstractIn these decades, synthesis of polymers with controlled monomer sequences has been of increasing importance in polymer chemistry. When we tested synthesis of a heterodimer of 4‐azido‐5‐hexynoic acid derivatives carrying t‐butyl (tBu) ester and tBu amide side chains for preparation of an alternating copolymer, we unexpectedly obtained a heterodimer precursor carrying tBu ester and nitrile side chains, in a high yield. Since dense 1,2,3‐triazole polymers carrying nitrile side chains are expected as a new functional polymer, the heterodimer is polymerized by copper(I)‐catalyzed azide–alkyne cycloaddition polymerization to obtain an alternating copolymer carrying tBu ester and nitrile side chains in this study. Since 1,2,3‐triazole and nitrile moieties are known to be ligands for metal ions, the complex formation of the alternating copolymer with PdCl2 is also investigated by NMR. In the presence of PdCl2, 1H NMR spectra show broad and separated signals due to the free and complexed copolymers, indicative of slow exchange. Pulse‐field‐gradient spin‐echo NMR data indicated that the coordination of alternating copolymer to PdCl2 made the copolymer chains take a more compact conformation under dilute conditions. The density functional theory calculations indicated that the alternating copolymer coordinated to PdCl2 preferentially through the 1,2,3‐triazole nitrogen and nitrile nitrogen atoms.