bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract B034: Order-of-mutation effects on cancer progression: Models for myeloproliferative neoplasm
Authors: Wang Yue , Chou Tom , Shtylla Blerta 
Published: February 2024

Abstract
In some patients with myeloproliferative neoplasms (MPN), two genetic mutations are often found, JAK2 V617F and one in the TET2 gene. Whether or not one mutation is present will influence how the other subsequent mutation affects the regulation of gene expression. When both mutations are present, the order of their occurrence has been shown to influence disease progression and prognosis. We propose a nonlinear ordinary differential equation (ODE) model and a Moran process models to explain the non-additive and non-commutative mutation effects on recent clinical observations of gene expression patterns, proportions of cells with different mutations, and ages at diagnosis of MPN. These observations consistently shape our modeling framework. Our key proposal is that bistability in gene expression provides a natural explanation for many observed order-of-mutation effects. We also propose potential experimental measurements that can be used to confirm or refute predictions of our models.
Citation Format: Yue Wang, Tom Chou, Blerta Shtylla. Order-of-mutation effects on cancer progression: Models for myeloproliferative neoplasm [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Cancer Evolution and Data Science: The Next Frontier; 2023 Dec 3-6; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(3 Suppl_2):Abstract nr B034.
Abstract B038: A preliminary study on predicting pathological complete response to neoadjuvant chemotherapy in triple-negative breast cancer
Authors: Chen Xi , Zhou Zhiguo 
Published: February 2024

Abstract
Purpose In this preliminary study, we are aiming to develop a deep learning model to predict pathological complete response (pCR) to neoadjuvant chemotherapy (NAC) for triple-negative breast cancer (TNBC) patient based on dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Background Neoadjuvant chemotherapy (NAC) has become a grown treatment method for patients with triple-negative breast cancer (TNBC). However, not every patient can achieve pathological complete response (pCR) to NAC. NAC also has side effects such as nausea, hair loss, appetite loss, mouth sores, and nerve damage. Additionally, delaying surgery to remove the tumor can allow it to spread, making treatment more difficult. Therefore, it is necessary to predict pCR to NAC reliably in a non-invasive way. Deep learning methods, especially convolutional neural network (CNN), have been shown great success in image-based treatment response prediction. Methods In this preliminary study, totally 78 TNBC patients on DCE-MRI collected from public dataset (Duke University) were used, among which are 23 patients with pCR and 55 patients without pCR. For each patient, the median slice from the tumor area of the fat-saturated gradient echo T1-weighted pre-contrast sequence MRI was extracted. A convolutional neural network model was built to predict whether the patients receive pCR or not after NAC. In the constructed prediction model, three convolutional layers were used to capture the features from DCE-MRI. Each convolutional layer was followed by a max-pooling layer to reduce the dimensions of the feature maps. Two fully connected layers were added into the model so that the model has a binary outcome which provides the prediction of pCR. Additionally, “ReLU” was used as the activation function in all the layers except for the output layer which used “softmax” instead. By using augmentation technique, we solved the problems of small sample size and unbalance data. Results In this study, 75% dataset were used for training and the remaining 25% were used for testing. The model we developed has a 75% accuracy, with a sensitivity of 0.67 and a specificity of 0.79. These results indicate that the model performs well in predicting patients who do not achieve pathological complete response (pCR). Additionally, the area under the receiver operating characteristic (AUC) curve is calculated as 0.74, indicating the outstanding performance.
Citation Format: Xi Chen, Zhiguo Zhou. A preliminary study on predicting pathological complete response to neoadjuvant chemotherapy in triple-negative breast cancer [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Cancer Evolution and Data Science: The Next Frontier; 2023 Dec 3-6; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(3 Suppl_2):Abstract nr B038.
Abstract IA020: Microphysiological systems to decipher tumor-stromal interactions in colorectal cancer
Authors: Mumenthaler Shannon 
Published: February 2024

Abstract
Cancer is a complex adaptive system driven by the intricate interplay between tumor cells and their microenvironment. Among these interactions, cancer-associated fibroblasts (CAFs), the predominant cellular component of the tumor stroma, are frequently linked with an unfavorable prognosis and play an important role in the progression of various cancers. Although extensive literature has underscored the impact of CAFs on cancer cell behaviors such as proliferation and invasion, the stromal influence on phenotypic plasticity in the context of treatment response and metastasis remains largely unexplored. Furthermore, preclinical treatment studies often concentrate on drug-induced changes in tumor cells with little investigation into the impact on surrounding stromal cells. To enhance our comprehension of the intricate and dynamic interactions between cancer cells and their microenvironment, we are utilizing quantitative high-content imaging in conjunction with more physiologically relevant patient-derived model systems, such as organoids and organ-on-chip technologies. Here we will describe our experimental workflows and demonstrate how the incorporation of mathematical modeling enhances our understanding of 3D organoid growth dynamics and facilitates a more detailed examination of drug resistance influenced by tumor plasticity. One illustrative example we will emphasize involves the treatment of CAFs with an anti-epidermal growth factor receptor (EGFR) targeted agent, cetuximab, resulting in an increased secretion of epidermal growth factor (EGF). This heightened EGF secretion was sufficient to render neighboring tumor cells resistant to cetuximab treatment. These efforts aim to increase our understanding of tumor-stromal interactions in cancer evolution, particularly emphasizing the functional and therapeutic potential of CAFs, through the integration of expertise across disciplines.
Citation Format: Shannon Mumenthaler. Microphysiological systems to decipher tumor-stromal interactions in colorectal cancer [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Cancer Evolution and Data Science: The Next Frontier; 2023 Dec 3-6; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(3 Suppl_2):Abstract nr IA020.
FIGURE 3 from Genome-wide p63-Target Gene Analyses Reveal TAp63/NRF2-Dependent Oxidative Stress Responses
Authors: Napoli Marco , Deshpande Avani A. , Chakravarti Deepavali , Rajapakshe Kimal , Gunaratne Preethi H. , Coarfa Cristian , Flores Elsa R. 
Published: February 2024

<p>∆Np63 and TAp63 control distinct biological processes by cooperating with different transcription factors. <b>A,</b> Venn diagrams of ΔNp63-specific, common, and TAp63-specific direct target genes, with p63 ChIP-seq peaks ± 10 kb from their genomic loci. <b>B–D,</b> List of the differentially enriched pathways in ΔNp63-specific, common, and TAp63-specific direct target genes. <b>E,</b> Table listing the top 10 differentially enriched motifs in the peaks ± 10 kb from ΔNp63-specific, common, and TAp63-specific direct target genes. Highlighted in yellow are the motifs of the transcription factors selected for validation.</p>
Spatial Metabolomics Identifies LPC(18:0) and LPA(18:1) in Advanced Atheroma With Translation to Plasma for Cardiovascular Risk Estimation
Authors: Cao Jianhua , Martin-Lorenzo Marta , van Kuijk Kim , Wieland Elias B. , Gijbels Marion J. , Claes Britt S.R. , Heredero Angeles , Aldamiz-Echevarria Gonzalo , Heeren Ron M.A. , Goossens Pieter , Sluimer Judith C. , Balluff Benjamin , Alvarez-Llamas Gloria 
Published: February 2024

BACKGROUND:
The metabolic alterations occurring within the arterial architecture during atherosclerosis development remain poorly understood, let alone those particular to each arterial tunica. We aimed first to identify, in a spatially resolved manner, the specific metabolic changes in plaque, media, adventitia, and cardiac tissue between control and atherosclerotic murine aortas. Second, we assessed their translatability to human tissue and plasma for cardiovascular risk estimation.

METHODS:
In this observational study, mass spectrometry imaging (MSI) was applied to identify region-specific metabolic differences between atherosclerotic (n=11) and control (n=11) aortas from low-density lipoprotein receptor–deficient mice, via histology-guided virtual microdissection. Early and advanced plaques were compared within the same atherosclerotic animals. Progression metabolites were further analyzed by MSI in 9 human atherosclerotic carotids and by targeted mass spectrometry in human plasma from subjects with elective coronary artery bypass grafting (cardiovascular risk group, n=27) and a control group (n=27).

RESULTS:

MSI identified 362 local metabolic alterations in atherosclerotic mice (log2 fold-change ≥1.5;
P
≤0.05). The lipid composition of cardiac tissue is altered during atherosclerosis development and presents a generalized accumulation of glycerophospholipids, except for lysolipids. Lysolipids (among other glycerophospholipids) were found at elevated levels in all 3 arterial layers of atherosclerotic aortas. LPC(18:0) (lysophosphatidylcholine;
P
=0.024) and LPA(18:1) (lysophosphatidic acid;
P
=0.025) were found to be significantly elevated in advanced plaques as compared with mouse-matched early plaques. Higher levels of both lipid species were also observed in fibrosis-rich areas of advanced- versus early-stage human samples. They were found to be significantly reduced in human plasma from subjects with elective coronary artery bypass grafting (
P
<0.001 and
P
=0.031, respectively), with LPC(18:0) showing significant association with cardiovascular risk (odds ratio, 0.479 [95% CI, 0.225–0.883];
P
=0.032) and diagnostic potential (area under the curve, 0.778 [95% CI, 0.638–0.917]).

CONCLUSIONS:
An altered phospholipid metabolism occurs in atherosclerosis, affecting both the aorta and the adjacent heart tissue. Plaque-progression lipids LPC(18:0) and LPA(18:1), as identified by MSI on tissue, reflect cardiovascular risk in human plasma.
Abstract TP224: Timing of Ischemic and Hemorrhagic Stroke With Myeloproliferative Neoplasm Diagnosis Among Veterans
Authors: Mathur Natasha , Tiu Andrew , McKinnell Zoe , Gill Puneet , Antonio Martha , Liu Shanshan , Diao Guoqing , Subrahmanyam Ramesh , Kessler Craig , Jain Maneesh 
Published: February 2024

Myeloproliferative Neoplasms (MPNs) are rare acquired stem cell disorders, consisting of Essential Thrombocythemia (ET), Polycythemia Vera (PV), and Primary Myelofibrosis (PMF), complicated by thrombohemorrhagic events such as stroke. The timing of ischemic stroke (IS) and hemorrhagic stroke (HS) in relation to MPN diagnosis among Veterans remains undefined.
We utilized the Veterans Affairs Informatics and Computing Infrastructure database from 1/1/2006 - 1/26/2023 and included 586,555 Veterans from Illinois, the state most representative of the US population. ICD-9 and -10 codes identified Veterans with stroke and MPN. Fisher’s exact test was utilized to compare MPNs vs. non-MPNs.
There were 237 MPNs and 15,221 non-MPNs with IS while there were 26 MPNs and 1,567 non-MPNs with HS (Table 1). There were no differences in age of stroke diagnosis among MPNs vs. non-MPNs. There were higher rates of hypertension, smoking, and heart failure among MPNs vs. non-MPNs with IS.
Majority of IS was diagnosed either more than 3 months before MPN diagnosis (N=115, 48.5%) or more than 5 years after diagnosis (N=98, 41.4%). Among MPNs with HS, stroke was predominantly diagnosed (N=14, 53.8%) more than 3 months before and (N=7, 26.9%) within 3 months of MPN diagnosis (Figure 1). Among MPNs there was recurrence of stroke among 45.18% of IS and 40% of HS.
Although MPN are infrequent causes of stroke, these findings suggest continued surveillance for MPN and workup for driver mutations after a stroke diagnosis. This study also suggests consideration for dual antiplatelet therapy for MPNs with cardiovascular risk factors to prevent IS and a multidisciplinary approach between neurology and hematology.
Abstract TP53: Above Par on the Back Nine: Improving Triage of Posterior Circulation Stroke
Authors: Beavis James , Lewis Maya 
Published: February 2024

Background and Purpose:
Notoriously difficult to recognize, posterior circulation strokes (PCS) can be some of the most devastating. It is incumbent on the triage nurse to recognize these signs and symptoms. This project was developed to educate frontline ED staff on early identification of posterior strokes, decrease time to recognition, and improve outcomes.

Methods:
The community Primary Stroke Center (PSC) identified the need to improve recognition of PCS after a series of treatment-eligible patients were missed. A team was created including the directors of the PSC and associated Comprehensive Stroke Center (CSC), emergency room physicians, and nurses. A triage algorithm was created to capture as many strokes as possible without a glut of false positives. This included focal and non-focal signs of stroke. ANY sign of stroke should initiate the code stroke (CS) process provided that the presentation was within 24 hours of last known well, the presentation was sudden, and the symptoms were disabling to the patient. Once approved, the algorithm and education were provided to all patient-facing ED staff. Updates were added to huddle notes and a copy of the algorithm was made available at all triage areas. Data was collected retroactively for a total of 16 months, 8 months prior to the intervention and 8 months after.

Results:

- For all PCS the median time from arrival to initiation of CS improved from 9 minutes to 1 minute
- For all PCS the median time from arrival to confirmation of stroke improved from 597 minutes to 249 minutes
- Prior to intervention, only 60% of CS-eligible patients had a CS initiated, compared to 92% after
- Ninety-day Rankin was from 0-2 in 86% of PCS patients prior to intervention compared to 100% after

Conclusions:
A clear triage process can maximize identification of PCS, increase capture of treatment-eligible patients, and decrease disability. A PSC with tele-stroke coverage and ongoing education improved identification of posterior circulation strokes and outcomes. Key takeaways for success include clear and concise code stroke algorithm, support from providers, and ongoing educational interventions. While this data is preliminary, we will continue to track and trend. Next steps should include education directed to EMS personnel in the field.
The Art of Communicative Grammar
Authors: Christenson David 
Published: February 2024

Abstract
This review article of the Oxford Latin Syntax (Vols. I-II, 2015 and 2021) demonstrates how the communicative approach of Harm Pinkster’s monumental grammar complements recent revivals of oral, aural, and active Latin pedagogical methods. I conclude with examples of how Pinkster’s analysis of discourse may be applied to select passages (from Plautus’ Rudens, as a productive means of explicating dramaturgical and narratological matters there) in order to illustrate the OLS’s manifold potential for exploring discursive strategies of Latin texts generally.
Performance Improvement Program Review of Institutional Massive Transfusion Protocol Adherence: An Opportunity for Improvement
Authors: Easterday Thomas , Byerly Saskya , Magnotti Louis , Fischer Peter , Shah Kinjal , Croce Martin , Kerwin Andrew , Howley Isaac 
Published: February 2024

BackgroundGiven the acuity of patients who receive MTPs and the resources they require, MTPs are a compelling target for performance improvement. This study evaluated adherence with our MTP’s plasma:red blood cell ratio (FFPR) of 1:2 and platelet:red blood cell ratio (PLTR) of 1:12, to test the hypothesis that ratio adherence is associated with lower inpatient mortality.Materials and MethodsThe registry of an urban level I trauma center was queried for adult patients who received at least 6 units of packed red blood cells within 4 hours of presentation. Patients were excluded for interfacility transfer, cardiac arrest during the prehospital phase or within one hour of arrival, or for head AIS ≥5. Univariate analysis and multiple logistic regressions were performed to identify variables associated with early transfusion protocol noncompliance and the effect on inpatient mortality.ResultsThree hundred and eighty-three patients were included, with mean ISS of 25.9 ± 13.3 and inpatient mortality of 28.5%. Increasing age, ISS, INR, and total units of blood product transfused were associated with increased odds of mortality, while an increase in revised trauma score was associated with a decreased odds ratio of mortality. Achieving our goal ratios were protective against mortality, with OR of .451 ( P = .013) and .402 ( P=.003), respectively.DiscussionLarge proportions of critically injured patients were transfused fewer units of plasma and platelets than our MTP dictated; failure to achieve intended ratios at 4 hours was strongly associated with inpatient mortality. MTP processes and outcomes should be critically assessed on a regular basis as part of a mature performance improvement program to ensure protocol adherence and optimal patient outcome.
Availability of E-resources in private agriculture collages in Uttar Pradesh, Bharat
Authors: Singh Varsha , Kharwar Vishwadeep , Singh Avinash Kumar 
Published: February 2024

Background Libraries support us by giving us the information we require, which is a very important part of their role in our lives. It is regarded as a group or establishment that assists us in effectively storing information and collects, protects, and disperses it to people that require it. The documents are gathered by the agricultural library, which then makes them accessible to its users by giving them the necessary details. Teachers, students, and researchers who study zoology, genetics, plant and animal breeding, anatomy and physiology, plant and animal pathology, entomology, soil science, horticulture, and agricultural economics are among the users of agricultural libraries. The agricultural library’s users must cater to a variety of patrons, including those with very specific needs, such teachers, and scientists. Area of Study 13 private agriculture colleges in Uttar Pradesh were chosen by the researcher for the study. Methodology Data were gathered using the survey method. 770 survey questionnaires were handed out. Findings Despite having a proper library, ICT infrastructure, and many other things, the study’s findings demonstrate that private agriculture colleges in Uttar Pradesh do not have a collection of electronic resources. The report makes several recommendations for the creation of libraries for private agriculture institutions in Uttar Pradesh.
Clinical outcomes of extensor indicis proprius tendon transfer for extensor pollicis longus tendon rupture
Authors: Ha Cheungsoo , Hong In-Tae , Oh Chi-Hoon , Ryu Han-Seung , Chung Jaiwoo , Han Soo-Hong 
Published: February 2024

The purpose of the present study was to investigate the clinical results of extensor indicis proprius tendon transfer for extensor pollicis longus tendon rupture, with a focus on objective measurement of extensor strength. We studied 40 patients who underwent extensor indicis proprius transfer with a minimum follow-up of 12 months. Outcome measurements included the Disabilities of the Arm, Shoulder and Hand (DASH) score, range of motion and grip power of the thumb and index finger. The thumb and index finger of the operated hand showed approximately 20% lower extensor power ( p < 0.001) compared to the non-operated hand at final follow-up. However, the DASH score significantly improved. The range of motion in the index finger was near normal, while there was an extension deficit in the thumb of 1.2 cm and a flexion deficit of 1.7 cm compared to the normal side. Despite the decrease in extension strength, clinical functional scores and range of motion were favourable. Level of evidence: IV
How Do Students Develop Creativity and Curiosity? The Role of Out-of-School Activities
Authors: Zhang (张静) Jing , Fan (范舒茗) Shuming , Huang (黄忠敬) Zhongjing 
Published: February 2024

Purpose This study investigates the degree to which students’ participation in out-of-school activities promotes their curiosity and creativity. In doing so, this study addresses the question of whether various out-of-school activities function differently in the development of students’ curiosity and creativity. Design/Approach/Methods This study sampled 3,647 students for the younger cohort and 3,621 students for the older cohort from the city of Suzhou, China. Findings Both younger and older students who participated in artistic activities and sports consistently rated their creativity and curiosity higher than those who did not. After controlling for family socio-economic status and gender, students’ participation in outdoor socially-oriented activities (e.g., meeting with friends outside school) and indoor activities (e.g., reading books) contributed to the development of creativity and curiosity. In contrast, participation in Internet-based activities negatively predicts adolescents’ creativity and curiosity. Originality/Value Creativity and curiosity are related to students’ academic performance and future success. Therefore, cultivating students’ creativity and curiosity should be of interest to educators and policymakers. This study examines the roles of different out-of-school activities on students’ creativity and curiosity, revealing a potential path for educators.